Calcium ionophores. Medical search

Chemical agents that increase the permeability of biological or artificial lipid membranes to specific ions. Most ionophores are relatively small organic molecules that act as mobile carriers within membranes or coalesce to form ion permeable channels across membranes. Many are antibiotics, and many act as uncoupling agents by short-circuiting the proton gradient across mitochondrial membranes.

Cationic ionophore antibiotic obtained from Streptomyces lasaliensis that, among other effects, dissociates the calcium fluxes in muscle fibers. It is used as a coccidiostat, especially in poultry.

An ionophorous, polyether antibiotic from Streptomyces chartreusensis. It binds and transports CALCIUM and other divalent cations across membranes and uncouples oxidative phosphorylation while inhibiting ATPase of rat liver mitochondria. The substance is used mostly as a biochemical tool to study the role of divalent cations in various biological systems.

A divalent calcium ionophore that is widely used as a tool to investigate the role of intracellular calcium in cellular processes.

Chemical agents that increase the permeability of CELL MEMBRANES to CALCIUM ions.

A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.

A chelating agent relatively more specific for calcium and less toxic than EDETIC ACID.

A phorbol ester found in CROTON OIL with very effective tumor promoting activity. It stimulates the synthesis of both DNA and RNA.

Signal transduction mechanisms whereby calcium mobilization (from outside the cell or from intracellular storage pools) to the cytoplasm is triggered by external stimuli. Calcium signals are often seen to propagate as waves, oscillations, spikes, sparks, or puffs. The calcium acts as an intracellular messenger by activating calcium-responsive proteins.

A polyether antibiotic which affects ion transport and ATPase activity in mitochondria. It is produced by Streptomyces hygroscopicus. (From Merck Index, 11th ed)

An antiprotozoal agent produced by Streptomyces cinnamonensis. It exerts its effect during the development of first-generation trophozoites into first-generation schizonts within the intestinal epithelial cells. It does not interfere with hosts' development of acquired immunity to the majority of coccidial species. Monensin is a sodium and proton selective ionophore and is widely used as such in biochemical studies.

Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.

A cyclododecadepsipeptide ionophore antibiotic produced by Streptomyces fulvissimus and related to the enniatins. It is composed of 3 moles each of L-valine, D-alpha-hydroxyisovaleric acid, D-valine, and L-lactic acid linked alternately to form a 36-membered ring. (From Merck Index, 11th ed) Valinomycin is a potassium selective ionophore and is commonly used as a tool in biochemical studies.

Voltage-dependent cell membrane glycoproteins selectively permeable to calcium ions. They are categorized as L-, T-, N-, P-, Q-, and R-types based on the activation and inactivation kinetics, ion specificity, and sensitivity to drugs and toxins. The L- and T-types are present throughout the cardiovascular and central nervous systems and the N-, P-, Q-, & R-types are located in neuronal tissue.

Calcium compounds used as food supplements or in food to supply the body with calcium. Dietary calcium is needed during growth for bone development and for maintenance of skeletal integrity later in life to prevent osteoporosis.

Chemical agents that increase the permeability of CELL MEMBRANES to POTASSIUM ions.

Carbonic acid calcium salt (CaCO3). An odorless, tasteless powder or crystal that occurs in nature. It is used therapeutically as a phosphate buffer in hemodialysis patients and as a calcium supplement.

A proton ionophore. It is commonly used as an uncoupling agent and inhibitor of photosynthesis because of its effects on mitochondrial and chloroplast membranes.

A proton ionophore that is commonly used as an uncoupling agent in biochemical studies.

Chemical agents that increase the permeability of CELL MEMBRANES to SODIUM ions.

Calcium salts of phosphoric acid. These compounds are frequently used as calcium supplements.

Stable calcium atoms that have the same atomic number as the element calcium, but differ in atomic weight. Ca-42-44, 46, and 48 are stable calcium isotopes.

A salt used to replenish calcium levels, as an acid-producing diuretic, and as an antidote for magnesium poisoning.

A group of peptide antibiotics from BACILLUS brevis. Gramicidin C or S is a cyclic, ten-amino acid polypeptide and gramicidins A, B, D are linear. Gramicidin is one of the two principal components of TYROTHRICIN.

The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)

The rate dynamics in chemical or physical systems.

An element in the alkali group of metals with an atomic symbol K, atomic number 19, and atomic weight 39.10. It is the chief cation in the intracellular fluid of muscle and other cells. Potassium ion is a strong electrolyte that plays a significant role in the regulation of fluid volume and maintenance of the WATER-ELECTROLYTE BALANCE.

The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization).

Long-lasting voltage-gated CALCIUM CHANNELS found in both excitable and nonexcitable tissue. They are responsible for normal myocardial and vascular smooth muscle contractility. Five subunits (alpha-1, alpha-2, beta, gamma, and delta) make up the L-type channel. The alpha-1 subunit is the binding site for calcium-based antagonists. Dihydropyridine-based calcium antagonists are used as markers for these binding sites.

The movement of materials across cell membranes and epithelial layers against an electrochemical gradient, requiring the expenditure of metabolic energy.

Compounds with a 5-membered ring of four carbons and an oxygen. They are aromatic heterocycles. The reduced form is tetrahydrofuran.

The calcium salt of oxalic acid, occurring in the urine as crystals and in certain calculi.

The calcium salt of gluconic acid. The compound has a variety of uses, including its use as a calcium replenisher in hypocalcemic states.

A member of the alkali group of metals. It has the atomic symbol Na, atomic number 11, and atomic weight 23.

The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.

Unstable isotopes of calcium that decay or disintegrate emitting radiation. Ca atoms with atomic weights 39, 41, 45, 47, 49, and 50 are radioactive calcium isotopes.

An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.

Lanthanum. The prototypical element in the rare earth family of metals. It has the atomic symbol La, atomic number 57, and atomic weight 138.91. Lanthanide ion is used in experimental biology as a calcium antagonist; lanthanum oxide improves the optical properties of glass.

A metallic element that has the atomic symbol Mg, atomic number 12, and atomic weight 24.31. It is important for the activity of many enzymes, especially those involved in OXIDATIVE PHOSPHORYLATION.

Electrodes which can be used to measure the concentration of particular ions in cells, tissues, or solutions.

Metallochrome indicator that changes color when complexed to the calcium ion under physiological conditions. It is used to measure local calcium ion concentrations in vivo.

Chemical agents that increase the permeability of CELL MEMBRANES to PROTONS.

Solution titration in which the end point is read from the electrode-potential variations with the concentrations of potential determining ions. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

A sesquiterpene lactone found in roots of THAPSIA. It inhibits CA(2+)-TRANSPORTING ATPASE mediated uptake of CALCIUM into SARCOPLASMIC RETICULUM.

Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.

A calcium channel blocker that is a class IV anti-arrhythmia agent.

Inorganic compounds that contain calcium as an integral part of the molecule.

Chemicals that bind to and remove ions from solutions. Many chelating agents function through the formation of COORDINATION COMPLEXES with METALS.

CALCIUM CHANNELS that are concentrated in neural tissue. Omega toxins inhibit the actions of these channels by altering their voltage dependence.

A suborder of the order ARTIODACTYLA whose members have the distinguishing feature of a four-chambered stomach, including the capacious RUMEN. Horns or antlers are usually present, at least in males.

A toxic dye, chemically related to trinitrophenol (picric acid), used in biochemical studies of oxidative processes where it uncouples oxidative phosphorylation. It is also used as a metabolic stimulant. (Stedman, 26th ed)

Drugs used by veterinarians in the treatment of animal diseases. The veterinarian's pharmacological armamentarium is the counterpart of drugs treating human diseases, with dosage and administration adjusted to the size, weight, disease, and idiosyncrasies of the species. In the United States most drugs are subject to federal regulations with special reference to the safety of drugs and residues in edible animal products.

A carbodiimide that is used as a chemical intermediate and coupling agent in peptide synthesis. (From Hawley's Condensed Chemical Dictionary, 12th ed)

Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.

Stable elementary particles having the smallest known positive charge, found in the nuclei of all elements. The proton mass is less than that of a neutron. A proton is the nucleus of the light hydrogen atom, i.e., the hydrogen ion.

2-Hydroxy-N-phenylbenzamides. N-phenyl substituted salicylamides. Derivatives have been used as fungicides, anti-mildew agents and topical antifungal agents. In concentrated form may cause irritation of skin and mucous membranes.

Agents that increase calcium influx into calcium channels of excitable tissues. This causes vasoconstriction in VASCULAR SMOOTH MUSCLE and/or CARDIAC MUSCLE cells as well as stimulation of insulin release from pancreatic islets. Therefore, tissue-selective calcium agonists have the potential to combat cardiac failure and endocrinological disorders. They have been used primarily in experimental studies in cell and tissue culture.

Agents useful in the treatment or prevention of COCCIDIOSIS in man or animals.

Substances that reduce the growth or reproduction of BACTERIA.

Elements of limited time intervals, contributing to particular results or situations.

Positively charged atoms, radicals or groups of atoms with a valence of plus 2, which travel to the cathode or negative pole during electrolysis.

Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.

A class of compounds composed of repeating 5-carbon units of HEMITERPENES.

An acidifying agent that has expectorant and diuretic effects. Also used in etching and batteries and as a flux in electroplating.

Quinolines substituted in any position by one or more amino groups.

Established cell cultures that have the potential to propagate indefinitely.

A quality of cell membranes which permits the passage of solvents and solutes into and out of cells.

The relationship between the dose of an administered drug and the response of the organism to the drug.

Chemical agents that uncouple oxidation from phosphorylation in the metabolic cycle so that ATP synthesis does not occur. Included here are those IONOPHORES that disrupt electron transfer by short-circuiting the proton gradient across mitochondrial membranes.

The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.

A highly poisonous compound that is an inhibitor of many metabolic processes, but has been shown to be an especially potent inhibitor of heme enzymes and hemeproteins. It is used in many industrial processes.

A chelating agent that sequesters a variety of polyvalent cations such as CALCIUM. It is used in pharmaceutical manufacturing and as a food additive.

Positively charged atoms, radicals or groups of atoms which travel to the cathode or negative pole during electrolysis.

A white powder prepared from lime that has many medical and industrial uses. It is in many dental formulations, especially for root canal filling.

Artificial, single or multilaminar vesicles (made from lecithins or other lipids) that are used for the delivery of a variety of biological molecules or molecular complexes to cells, for example, drug delivery and gene transfer. They are also used to study membranes and membrane proteins.

A non-metal element that has the atomic symbol P, atomic number 15, and atomic weight 31. It is an essential element that takes part in a broad variety of biochemical reactions.

The first stomach of ruminants. It lies on the left side of the body, occupying the whole of the left side of the abdomen and even stretching across the median plane of the body to the right side. It is capacious, divided into an upper and a lower sac, each of which has a blind sac at its posterior extremity. The rumen is lined by mucous membrane containing no digestive glands, but mucus-secreting glands are present in large numbers. Coarse, partially chewed food is stored and churned in the rumen until the animal finds circumstances convenient for rumination. When this occurs, little balls of food are regurgitated through the esophagus into the mouth, and are subjected to a second more thorough mastication, swallowed, and passed on into other parts of the compound stomach. (From Black's Veterinary Dictionary, 17th ed)

Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.

A potent vasodilator agent with calcium antagonistic action. It is a useful anti-anginal agent that also lowers blood pressure.

A potentially neurotoxic 8-hydroxyquinoline derivative long used as a topical anti-infective, intestinal antiamebic, and vaginal trichomonacide. The oral preparation has been shown to cause subacute myelo-optic neuropathy and has been banned worldwide.

A calcium salt that is used for a variety of purposes including: building materials, as a desiccant, in dentistry as an impression material, cast, or die, and in medicine for immobilizing casts and as a tablet excipient. It exists in various forms and states of hydration. Plaster of Paris is a mixture of powdered and heat-treated gypsum.

The concentration of osmotically active particles in solution expressed in terms of osmoles of solute per liter of solution. Osmolality is expressed in terms of osmoles of solute per kilogram of solvent.

Organic compounds that contain two nitro groups attached to a phenol.

A polypeptide hormone (84 amino acid residues) secreted by the PARATHYROID GLANDS which performs the essential role of maintaining intracellular CALCIUM levels in the body. Parathyroid hormone increases intracellular calcium by promoting the release of CALCIUM from BONE, increases the intestinal absorption of calcium, increases the renal tubular reabsorption of calcium, and increases the renal excretion of phosphates.

An atom or group of atoms that have a positive or negative electric charge due to a gain (negative charge) or loss (positive charge) of one or more electrons. Atoms with a positive charge are known as CATIONS; those with a negative charge are ANIONS.

Artificially produced membranes, such as semipermeable membranes used in artificial kidney dialysis (RENAL DIALYSIS), monomolecular and bimolecular membranes used as models to simulate biological CELL MEMBRANES. These membranes are also used in the process of GUIDED TISSUE REGENERATION.

An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.

Organic compounds containing the carboxy group (-COOH). This group of compounds includes amino acids and fatty acids. Carboxylic acids can be saturated, unsaturated, or aromatic.

Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.

A fluorescent calcium chelating agent which is used to study intracellular calcium in tissues.

Changes in content and localization of proteins phosphorylated at tyrosine, serine and threonine residues during ram sperm capacitation and acrosome reaction. (1/68)

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The use of anti-VDAC2 antibody for the combined assessment of human sperm acrosome integrity and ionophore A23187-induced acrosome reaction. (2/68)

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Ultrastructure and intracellular calcium response during activation in vitrified and slow-frozen human oocytes. (3/68)

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Effects of FK506 and cyclosporin a on calcium ionophore-induced mitochondrial depolarization and cytosolic calcium in astrocytes and neurons. (4/68)

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Calpain-cleaved type 1 inositol 1,4,5-trisphosphate receptor (InsP(3)R1) has InsP(3)-independent gating and disrupts intracellular Ca(2+) homeostasis. (5/68)

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Activation of human vascular cells decreases their expression of transforming growth factor-beta. (6/68)

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Chromatin disruption in the promoter of bovine leukemia virus during transcriptional activation. (7/68)

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The redox state of transglutaminase 2 controls arterial remodeling. (8/68)

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Calcium ionophores are also ideal to induce capacitation. Adding heparin to capacitation inducing medium mimics the secretion ... Calcium chloride is added to facilitated the influx via of calcium cations. In animal models, Tyrode's albumin lactate pyruvate ...

https://en.wikipedia.org/wiki/Capacitation

Fenstein, MB; Fraser, C (1975). "Human platelet secretion and aggregation induced by calcium ionophores. Inhibition by PGE1 and ... IP receptors bind with ionophores that induce ADP and serotonin secretion. PGE1 inhibits the secretion of factors that ... 5-triphosphate signaling and the mobilization of calcium inside the cell for thromboxane A2. PGI2, prostacyclin, binds to IP ...

https://en.wikipedia.org/wiki/Thromboregulation

... however others have found it necessary to also use calcium ionophore treatment for fertilisation to be successful. Calcium ... "Mechanism of action of calcium ionophores on intact cells: ionophore-resistant cells". Membrane & Cell Biology. 13 (3): 357-368 ... It has been found that treating globozoospermia with ICSI along with oocyte activation by calcium ionophore (an ion carrier ... "A Successful Healthy Childbirth in a Case of Total Globozoospermia with Oocyte Activation by Calcium Ionophore". Journal of ...

https://en.wikipedia.org/wiki/Globozoospermia

Jamil, K.; White, I. G. (December 1981). "Induction of acrosomal reaction in sperm with ionophore A23187 and calcium". Archives ... as well as the more commonly used calcium ionophore A23187. This can be done to serve as a positive control when assessing the ...

https://en.wikipedia.org/wiki/Acrosome

... and calcium ionophore-induced arachidonic acid release". J. Biol. Chem. 278 (40): 38206-13. doi:10.1074/jbc.M304816200. PMID ... CERK was later confirmed to bind calmodulin in the presence of calcium, indicating the calmodulin first binds calcium and then ... A calcium-stimulated lipid kinase that co-purifies with brain synaptic vesicles". J. Biol. Chem. 264 (24): 14354-60. doi: ... CERK has also been found to participate in the calcium-dependent degranulation of mast cells. Sugiura M, Kono K, Liu H, ...

https://en.wikipedia.org/wiki/Ceramide_kinase

Lee AS (January 1987). "Coordinated regulation of a set of genes by glucose and calcium ionophores in mammalian cells". Trends ... This calcium then interacts with calcineurin and calcium/calmodulin-dependent kinases that in turn activate transcription ... Muscular contraction during exercise causes calcium to be released from the sarcoplasmic reticulum (SR), a specialized ER ... calcium that is stored bound to resident molecular chaperones; and redox buffers that maintain the oxidizing environment ...

https://en.wikipedia.org/wiki/Unfolded_protein_response

Asaga H, Yamada M, Senshu T (February 1998). "Selective deimination of vimentin in calcium ionophore-induced apoptosis of mouse ...

https://en.wikipedia.org/wiki/Anti–citrullinated_protein_antibody

Calcium Ionophore, Antibiotic A23187 and Calcium Ionophore A23187. It is produced at fermentation of Streptomyces ... The ionophore is used in laboratories to increase intracellular Ca2+ levels in intact cells. It also uncouples oxidative ... It also acts as a divalent cation ionophore, allowing these ions to cross cell membranes, which are usually impermeable to them ... of Topotecan Enhances Anticancer Efficacy in Murine and Human Xenograft Models Cancer Research Effect of calcium ionophore on ...

https://en.wikipedia.org/wiki/A23187

ARE-directed mRNA degradation is influenced by many exogenous factors, including phorbol esters, calcium ionophores, cytokines ...

https://en.wikipedia.org/wiki/AU-rich_element

... is an ionophore and an antibiotic that binds calcium ions (Ca2+) in a ratio 1:1. It is produced by the bacterium ... Its β-diketone and carboxylic acid group form a chelate with calcium. It was extracted in 1978 and the complete structure was ... It is used in research to raise the intracellular calcium level (Ca2+) and as a research tool to understand Ca2+ transport ... 1979). "Structure of ionomycin - a novel diacidic polyether antibiotic having high affinity for calcium ions". Journal of the ...

https://en.wikipedia.org/wiki/Ionomycin

Reiss, Hans-Dieter; Herth, Werner (1979). "Calcium ionophore A 23187 affects localized wall secretion in the tip region of ...

https://en.wikipedia.org/wiki/Lilium_longiflorum

"Epidermal growth factor induces p11 gene and protein expression and down-regulates calcium ionophore-induced arachidonic acid ... "Epidermal growth factor induces p11 gene and protein expression and down-regulates calcium ionophore-induced arachidonic acid ... S100 calcium-binding protein A10 (S100A10), also known as p11, is a protein that is encoded by the S100A10 gene in humans and ... Wu T, Angus CW, Yao XL, Logun C, Shelhamer JH (Jul 1997). "P11, a unique member of the S100 family of calcium-binding proteins ...

https://en.wikipedia.org/wiki/S100A10

... may be artificially facilitated by calcium ionophores, something that is speculated to be useful in case of ... The slow block is through a biochemical mechanism triggered by a wave of calcium increase. The rise of calcium is both ... "Roscovitine in combination with calcium ionophore induces oocyte activation through reduction of M-phase promoting factor ... Sperm entry causes calcium release into the oocyte. In mammals, this is caused by the introduction of phospholipase C isoform ...

https://en.wikipedia.org/wiki/Oocyte_activation

Saunders, M. J.; P. K. Hepler (1982). "Calcium ionophore A23187 stimulates cytokinin-like mitosis in Funaria". Science. 217 ( ... This calcium binding may account for the bulk of the observed extracellular current. The intracellular calcium gradient may ... He has shown that calcium ions and protons are essential for growth. The intracellular free calcium ions exist in a gradient ... The delay between the extracellular and intracellular calcium peaks indicates that calcium ions do not immediately enter the ...

https://en.wikipedia.org/wiki/Peter_K._Hepler

The anergy in T cells can be induced by Ionomycin, the ionophore capable of raising intracellular concentration of calcium ions ... Under this condition, calcium dependent phosphatase calcineurin removes phosphates from a transcriptional factor NFAT, which in ... On the contrary, TCR signalling without costimulatory receptors sufficiently activates only the calcium arm of the signalling ... calcium dependent arm of a lymphocyte signalling is activated by TCR. This leads to an elevation of intracellular Ca+II ...

https://en.wikipedia.org/wiki/Clonal_anergy

Interference from calcium, magnesium, or lithium, on the other hand, is weak enough that their presence in normal ... Ionophores for potassium-selective electrodes v t e (Electrodes, All stub articles, Electrochemistry stubs). ... These electrodes are typical ion exchange resin membrane electrodes, using valinomycin, a potassium ionophore, as the ion ... calcium, magnesium, and lithium. The most significant interference with measurement of potassium concentration is from the ...

https://en.wikipedia.org/wiki/Potassium_selective_electrode

UTR of G-CSF mRNA contains a destablizing element that is insensitive to calcium ionophore, hence SLDE regulates G-CSF mRNA. ...

https://en.wikipedia.org/wiki/G-CSF_factor_stem-loop_destabilising_element

Sperm activation has been shown to be caused by calcium ionophores in vitro, progesterone released by nearby cumulus cells and ... The opening of CatSper channels is responsible for the influx of calcium. The sudden rise in calcium levels causes the ... A recent discovery links hyperactivation to a sudden influx of calcium ion into the tails. The whip-like tail (flagellum) of ... These channels are selective, allowing only calcium ions to pass. ...

https://en.wikipedia.org/wiki/Spermatozoon

https://en.wikipedia.org/wiki/Hyperactivation

... slow and inert in the reproductive tracts or storage areas of the female and are only activated by calcium ionophores in vitro ...

https://en.wikipedia.org/wiki/Cryptic_female_choice

PCP-2 mRNA is regulated by phorbol myristate acetate (PMA) or calcium ionophore, okadaic acid, the Ras inhibitor manumycin, and ... down-regulation of gene expression by PMA and calcium ionophore in Jurkat T lymphoma cells". Biochem Biophys Res Commun. 231 (1 ...

https://en.wikipedia.org/wiki/PTPRU

... of agents such as the chelator EGTA and the ionophore A23187 could have been incorrectly said to be specific for calcium in ... The traditional supply of calcium salts to fungal cultures may have met the need for trace elements as even A.R. calcium salts ... In the experiments with oxygen and hyphal development there was no requirement for calcium and no inhibition by EGTA. At the ... time of these studies many mycologists thought that calcium played a role in fungal morphology and were disinclined to believe ...

https://en.wikipedia.org/wiki/Allomyces_macrogynus

... calcium channel agonists MeSH D27.505.519.562.249 - calcium channel blockers MeSH D27.505.519.562.374 - ionophores MeSH D27.505 ... calcium channel agonists MeSH D27.505.954.411.793.610 - nasal decongestants MeSH D27.505.954.411.918 - vasodilator agents MeSH ... calcium channel blockers MeSH D27.505.954.411.207 - cardioplegic solutions MeSH D27.505.954.411.222 - cardiotonic agents MeSH ...

https://en.wikipedia.org/wiki/List_of_MeSH_codes_(D27)

... scrambling and do not express negatively charged phospholipids on their surface even after treatment with calcium ionophores. ...

https://en.wikipedia.org/wiki/COAT_platelet_defect

... down-regulation of gene expression by PMA and calcium ionophore in Jurkat T lymphoma cells". Biochemical and Biophysical ... Flentke GR, Garic A, Amberger E, Hernandez M, Smith SM (July 2011). "Calcium-mediated repression of β-catenin and its ... Soluble inositol-(1,4,5)-trisphosphate triggers calcium to be released from the endoplasmic reticulum. This sudden increase in ... "Modification of the composition of polycystin-1 multiprotein complexes by calcium and tyrosine phosphorylation". Biochimica et ...

https://en.wikipedia.org/wiki/Catenin_beta-1

The vibrating ion-selective microelectrode was first used in 1990 to measure calcium fluxes in various cells and tissues. The ... Only the specific ion permeates the ionophore, therefore the voltage readout is proportional to the ion concentration in the ... Some ions (such as calcium) and molecules (such as hydrogen peroxide) modulate targeted translocators to produce a current or ... ionophore) is tip-filled into a previously silanized (to prevent leakage) microelectrode. Also, the microelectrode vibrates at ...

https://en.wikipedia.org/wiki/Bioelectricity

... such as calcium ionophore treatment, microinjection of calcium ions, or electrical stimulation. Treatment with cycloheximide, a ... The fertilization event causes intracellular calcium oscillations, and targeted degradation of cyclin B, a regulatory subunit ...

https://en.wikipedia.org/wiki/Parthenogenesis

... and a lipid-soluble molecule that transports calcium ions (Ca2+) across the bilayer, also known as calcium ionophore. C1P was ... Inside the cell, S1P can induce calcium release independent of the S1PRs-the mechanism of which remains unknown. To date, the ... Tornquist, K. (February 2003). "Ceramide-1-phosphate increases intracellular free calcium concentrations in thyroid FRTL-5 ... and increase intracellular free calcium concentrations in thyroid cells. C1P also has known roles in vesicular trafficking, ...

https://en.wikipedia.org/wiki/Lipid_signaling

... and did not affect the release of glutamate evoked by a calcium ionophore (ionomycin). A polypeptide (Tx3-3) present in the ... This effect was most likely due to interference with the entry of calcium through voltage-gated calcium channels, because PhTx3 ... The PhTx3 neurotoxin is a broad-spectrum calcium channel blocker that inhibits glutamate release, calcium uptake and also ... We compared the alterations produced by the Tx3-3 with the actions of toxins known to block calcium channels coupled to ...

https://en.wikipedia.org/wiki/Phoneutria_nigriventer_toxin-3

Calcium Ionophore) Abamectine Abietic acid Acetic acid Acetylcholine Actin Actinomycin D Adenine Adenosmeme Adenosine ...

https://en.wikipedia.org/wiki/List_of_biomolecules

... is a member of a family of naturally occurring cyclic ionophores known as the macrotetrolide antibiotics. The other ... Vishwanath, C. K.; Shamala, N.; Easwaran, K. R. K.; Vijayan, M. (1983). "Structure of nonactin-calcium perchlorate, C40H64O12. ... This ion selectivity is seen in other macrocyclic ligands, such as the cyclic ionophore valinomycin, which is also an ... Due to this property, nonactin is also called "ammonium ionophore". During complexation, the nonactin backbone convolutes into ...

https://en.wikipedia.org/wiki/Nonactin

... which releases calcium. This leads to an increase in mitochondrial calcium, dropping its membrane potential. The pore ... a polypeptide from the Red Sea Moses sole with ionophore activity". FEBS Letters. 242 (1): 161-6. doi:10.1016/0014-5793(88) ...

https://en.wikipedia.org/wiki/Pardaxin

ISBN 978-3-527-30673-2. Sperelakis, Nicholas; Sperelakis, Nick (January 11, 2012). "Chapter 4: Ionophores in Planar Lipid ... mainly calcium, potassium, sodium, magnesium, iron, and manganese) by weight. Wood also contains sulfur, chlorine, silicon, ...

https://en.wikipedia.org/wiki/Wood

It also localizes to the acrosomal membrane of the sperm, where it putatively mediates calcium ion transmembrane transport. The ... "The use of anti-VDAC2 antibody for the combined assessment of human sperm acrosome integrity and ionophore A23187-induced ... In mammalian cardiomyocytes, VDAC2 promotes mitochondrial transport of calcium ions in order to power cardiac contractions. In ... Cell Calcium. 49 (2): 136-43. doi:10.1016/j.ceca.2010.12.004. PMID 21241999. Alvira CM, Umesh A, Husted C, Ying L, Hou Y, Lyu ...

https://en.wikipedia.org/wiki/VDAC2

... calcium ions are abnormally released. It is because of this that the calcium can become free-roaming which then can trigger ... Ingesting ionophores toxins, such as those found in day blooming jasmine, can cause the heart muscle to become inflamed, which ... When contracting muscles release intercellular calcium, however, if the horse has a predisposed condition that allows for ...

https://en.wikipedia.org/wiki/Equine_exertional_rhabdomyolysis

... a novel diacidic polyether antibiotic having high affinity for calcium ions". Journal of the American Chemical Society. 101 (12 ... unlike carrier ionophores. Examples of channel-forming ionophores are gramicidin A and nystatin. Ionophores that transport ... Ionophores can be selective to a particular ion but may not be exclusive to it. Ionophores facilitate the transport of ions ... However, these ionophores become unable to transport ions under very low temperatures. An example of a carrier ionophore is ...

https://en.wikipedia.org/wiki/Ionophore

... this calcium ion sensing ability is accommodated by an intracellular tail region on Slo-like subunits that form a calcium ion ... Ionophore and anthelmintic activity of PF 1022A, a cyclooctadepsipeptide, are not related - Gesner et al. Willson J; Amliwala K ... Role of calcium in neurotransmitter release evoked by alpha-latrotoxin or hypertonic sucrose - Khvotchev et al. A possible ... Schreiber M, Salkoff L (1997). "A novel calcium-sensing domain in the BK channel". Biophys. J. 73 (3): 1355-63. Bibcode:1997BpJ ...

https://en.wikipedia.org/wiki/Emodepside

The essential metals that lead interacts with include calcium, iron, and zinc. High levels of calcium and iron tend to provide ... A specific ion-selective electrode has been developed based on the ionophore S,S'-methylenebis (N,N-diisobutyldithiocarbamate ... Acute cases may require the use of disodium calcium edetate, the calcium chelate, and the disodium salt of ... By mimicking calcium, lead can cross the blood-brain barrier. It degrades the myelin sheaths of neurons, reduces their numbers ...

https://en.wikipedia.org/wiki/Lead

Family 1.A.106 The Calcium Load-activated Calcium Channel (CLAC) Family 1.A.107 The Pore-forming Globin (Globin) Family 1.B.1 ... Family 2.B.14 The Lasalocid A or X-537A Ionophore (Lasalocid) Family 2.B.15 The Tris-thiourea Tripodal-based Chloride Carrier ( ... Family 1.A.87 The Mechanosensitive Calcium Channel (MCA) Family 1.A.88 The Fungal Potassium Channel (F-Kch) Family 1.A.89 The ... Transportan Family 2.B.11 The Calcimycin or A23187 Carrier-type Ionophore Family 2.B.12 The Salinomycin Family 2.B.13 The ...

https://en.wikipedia.org/wiki/Transporter_Classification_Database

Similar solutions are formed by the heavy divalent alkaline earth metals calcium, strontium, barium, as well as the divalent ... carbohydrates and ionophore complexes. Lithium naturally only occurs in traces in biological systems and has no known ... Lithium and sodium are typically isolated through electrolysis from their liquid chlorides, with calcium chloride typically ... calcium (1.5%), and aluminium (1.4%); with the remaining 1.2% consisting of trace amounts of other elements. Due to planetary ...

https://en.wikipedia.org/wiki/Alkali_metal

When calcium binds to the troponin C, it causes conformational changes which lead to dislocation of troponin I. Afterwards, ... In veterinary medicine, increased cTnI has been noted from myocardial damage after ionophore toxicity in cattle. The high ... These changes alter the myofilament response to calcium, and are of interest in targeting heart failure. Multiple reaction ...

https://en.wikipedia.org/wiki/Troponin_I

416 Xenbase articles contain a reference to calcium ionophore according to textpresso ... Retrieved from "https://wiki.xenbase.org/xenwiki/index.php?title=Calcium_ionophore&oldid=47886" ...

https://wiki.xenbase.org/xenwiki/index.php/Calcium_ionophore

https://en.wikipedia.org/wiki/Capacitation

Calcium ionophore pre-treatment induces type-1 polarized DCs with enhanced T cell stimulatory function and IL-12 production. ...

https://jitc.biomedcentral.com/articles/10.1186/2051-1426-1-S1-P199/metrics

When the sample buffer mixture contacts the electrode, calcium ions complex with the ionophore at the electrode surface. ... LBXSCA - Total calcium (mg/dL). Variable Name: LBXSCA. SAS Label: Total calcium (mg/dL). English Text: Total calcium (mg/dL). ... LBDSCASI - Total calcium (mmol/L). Variable Name: LBDSCASI. SAS Label: Total calcium (mmol/L). English Text: Total calcium ( ... 7. Calcium The LX20 system uses indirect (or diluted) ISE methodology to measure calcium concentration in serum, plasma, or ...

https://wwwn.cdc.gov/Nchs/Nhanes/2005-2006/BIOPRO_D.htm

2016). Transient exposure to calcium ionophore enables in vitro fertilization in sterile mouse models. Scientific Reports, 6. ... 2015). Biphasic Role of Calcium in Mouse Sperm Capacitation Signaling Pathways. J Cell Physiol. presented at the 2015 Jan 17. ... 2000). S100P calcium-binding protein overexpression is associated with immortalization of human breast epithelial cells in ...

https://www.umass.edu/veterinary-animal-sciences/research-faculty/ana-maria-salicioni

Calcium ionophore-stimulated chemiluminescence (CL) from human granulocytes: evidence that A23187-induced chemiluminescence ...

http://www2a.cdc.gov/nioshtic-2/BuildQyr.asp?s1=hydrogen+peroxide&f1=%2A&Adv=0&terms=1&PageNo=207&RecNo=207&View=b&

Regulation of apoptosis in IL-3 dependent hemopoetic cells by IL-3 and calcium ionophores. ...

https://www.cell.com/cell/fulltext/0092-8674

Chapter 23: Calcium Indicators and Ionophores. *Chapter 24: Measuring Cells Millions at a Time (FACS -Analysis) ...

https://www.pearson.com/store/en-us/pearsonplus/p/9780137441778.html

Calcium Ionophore 25% Biochemistry, Genetics and Molecular Biology. * Acetyltransferase 100% * Normal Human 37% ...

https://research-repository.uwa.edu.au/en/publications/acetyl-coa-lyso-platelet-activating-factor-acetyltransferase-acti/fingerprints/

... mice and that ATP contributes to NETosis induced in vitro by the calcium ionophore A23187 or phorbol 12-myristate 13-acetate ( ... NADPH-independent NETosis may not be enhanced by ARL67156 due to the already maximal effect caused by the calcium ionophore. In ... 3C,D), confirming that NETosis induced by the calcium ionophore was already maximal. Interestingly, we observed that adding ATP ... mice and that ATP contributes to NETosis induced in vitro by the calcium ionophore A23187 or phorbol 12-myristate 13-acetate ( ...

https://www.nature.com/articles/s41598-019-53058-9

Calcium ionophores and CamKII over-expression affect mitotic stability leading to centrosome over-duplication (Matsumoto and ... Matsumoto, Y., and Maller, J. L. (2002). Calcium, calmodulin, and CaMKII requirement for initiation of centrosome duplication ... Matsumoto and Maller (2002) demonstrated the relation between calcium flux, CamKII activity, and centrosome duplication. ... showing calcium-independent calmodulin-binding properties (Ahmad et al., 2001). Using a truncation mutant Cx32D215 they ...

https://www.frontiersin.org/articles/10.3389/fnins.2014.00151/full

Calcimycin, Calcium ionophore A23187. Gene, Accession, CAS #. CAS: 52665-69-7. Catalog #. A-600. ... Purity >98%. A23187 is a mobile-carrier Ca2+ ionophore and can cause cell activation, differentiation, or proliferation and ...

https://www.linscottsdirectory.com/products/alomone-labs/dfae9b602010edb5abf7998854dc7a7fbb644503?rs=1

Calcium-induced insulin release in monolayer culture of the endocrine pancreas. Studies with ionophore A23187 Wollheim, Claes; ... Inhibition of insulin release by somatostatin: no evidence for interaction with calcium Wollheim, Claes; Blondel, B; Kikuchi, M ... Somatostatin inhibition of pancreatic glucagon release from monolayer cultures and interactions with calcium Wollheim, Claes; ...

https://archive-ouverte.unige.ch/documents/facets/sort:Document.year/direction:desc?newFacet=subjectFacet%3Dbarium%2Fpharmacology

Calcium ion levels in the capacitated sperm were reduced to a level similar to that in uncapacitated sperm after 90 min of ... Oviductal sperm was readily induced to undergo the acrosome reaction using the A23187 ionophore; however, the acrosome reaction ... Store-operated calcium entry (SOCE), a fundamentally important homeostatic and Ca2+ signaling pathway in many types of cells, ... Store-operated calcium entry (SOCE), a fundamentally important homeostatic and Ca2+ signaling pathway in many types of cells, ...

https://www.mdpi.com/1422-0067/19/5

Activation of sea-urchin eggs by a calcium ionophore. Proc. Natl. Acad. Sci. USA ... Ionophore activated eggs (white circle); eggs treated with U0126 15 minutes before ionophore activation (black circle). ... Ionophore activated eggs (white circle); eggs treated with U0126 15 minutes before ionophore activation (black circle). ... The relationship between calcium, MAP kinase, and DNA synthesis in Sea Urchin egg at fertilization. Dev. Biol. ...

https://journals.biologists.com/dev/article/132/3/579/43082/ERK1-activation-is-required-for-S-phase-onset-and

Histamine release in response to the calcium ionophore calcimycin (A23187) was not affected by the extract suggesting that a Ca ...

http://ispub.com/IJAAI/6/1/10898

Exposure of neurons to a calcium ionophore prevented axonogenesis. Confocal images of intracellular calcium levels and ... Exposure of neurons to a calcium ionophore prevented axonogenesis. Confocal images of intracellular calcium levels and ... Exposure of neurons to a calcium ionophore prevented axonogenesis. Confocal images of intracellular calcium levels and ... Exposure of neurons to a calcium ionophore prevented axonogenesis. Confocal images of intracellular calcium levels and ...

https://jhu.pure.elsevier.com/en/publications/evidence-for-mitochondrial-control-of-neuronal-polarity-3

... was evaluated by the capacity of sperm to undergo the acrosome reaction initiated by the addition of the calcium ionophore, ...

https://bioone.org/journals/biology-of-reproduction/volume-76/issue-3

... examined the effects of calcium influx on mCherry-BRAG1 distribution in live HeLa cells stimulated with the calcium ionophore, ... The calcium-dependent release of CaM from BRAG1 suggests that changes in intracellular calcium levels may regulate the BRAG1- ... Thus, high calcium and magnesium bath solution (29 ± 1.5°C), containing the following (in mm): 119 NaCl, 2.5 KCl, 4 CaCl2, 4 ... C, Calcium-induced condensation of BRAG1 is reversible. HeLa cells were either left untreated (left), were treated with 5 μm ...

https://www.jneurosci.org/content/32/34/11716?ijkey=b40c3c54fcc53a8dbbe9ebac92639dce4746b050&keytype2=tf_ipsecsha

Cell death was induced by the calcium ionophore A23187, selecting a dose that reduces the MTT assay readout by 50% (1 μM). The ...

http://www.molvis.org/molvis/v27/151/mv-v27-151-f1.html

A23187 is a calcium ionophore that causes a massive influx of calcium and nigericin stimulates potassium effluxes in cells ... the calcium ionophore A23187 which is produced during the growth of Streptomyces chartreusensis, (3) the potassium ionophore ... the calcium ionophore A23187, (3) the bacterial toxin nigericin that acts as a potassium ionophore, (4) the dimorphic fungus ... We examined the NETosis pathways induced by five stimuli; PMA, the calcium ionophore A23187, nigericin, Candida albicans and ...

https://elifesciences.org/articles/24437

... concentration was determined in gastric mucosa and in whole blood after ex vivo stimulation with a calcium ionophore. ...

https://avmajournals.avma.org/search?f_0=author&q_0=Lisa+Reynolds&print

Double Ionophore Application to Improve Blastocyst Development & Pregnancy Rate. Double exposure to calcium ionophore may ...

https://www.planer.com/blog/1293-delayed-childbearing-and-medically-assisted-reproduction.html

In contrast, the calcium-activated chloride current was not affected by antisense treatment. Hence, the cAMP and calcium ... We show here that Ca2+ ionophore activates Cl- currents in cell-attached and whole-cell patch-clamp recordings of Jurkat T ... RECOMBINANT HUMAN TUMOR-NECROSIS-FACTOR-ALPHA INDUCES CALCIUM OSCILLATION AND CALCIUM-ACTIVATED CHLORIDE CURRENT IN HUMAN ... CALCIUM AND LYMPHOCYTE-T ACTIVATION CELL Gardner, P. 1989; 59 (1): 15-20 Abstract. A prolonged (at least 2-4 hr) elevation of [ ...

https://profiles.stanford.edu/phyllis-gardner

The calcium ionophore, A23187, on the other hand, produced relaxation of the PV, which was significantly decreased by removing ...

https://jpet.aspetjournals.org/content/259/1/71

A Successful Healthy Childbirth in a Case of Total Globozoospermia with Oocyte Activation by Calcium Ionophore - Beyond the ...

https://www.urotoday.com/beyond-the-abstracts/men-s-health/male-infertility.html?start=0

... concentration was determined in gastric mucosa and in whole blood after ex vivo stimulation with a calcium ionophore. ...

https://avmajournals.avma.org/search?f_0=author&pageSize=10&q_0=Steven+C.+Budsberg&sort=relevance&print

Calcium ionophore-stimulated chemiluminescence (CL) from human granulocytes: evidence that A23187-induced chemiluminescence originates from arachidonic acid metabolism. (cdc.gov)
Here, we show that the ATP channel pannexin1 (Panx1) is functionally expressed by bone marrow-derived neutrophils (BMDNs) of wild-type (WT) mice and that ATP contributes to NETosis induced in vitro by the calcium ionophore A23187 or phorbol 12-myristate 13-acetate (PMA). (nature.com)
A23187 is a mobile-carrier Ca2+ ionophore and can cause cell activation, differentiation, or proliferation and thus mimics cellular processes observed in response to cytokines and growth factors.Furthermore, A23187 induces in a concentration and time-dependent manner, Ca2+ triggered cell apoptosis. (linscottsdirectory.com)
Cell death was induced by the calcium ionophore A23187, selecting a dose that reduces the MTT assay readout by 50% (1 μM). (molvis.org)
The calcium ionophore, A23187, on the other hand, produced relaxation of the PV, which was significantly decreased by removing the endothelium. (aspetjournals.org)
Then, the cells were treated for 2 hours to stimulate the protein kinase C (PKC) pathway via PGF2α and phorbol 12-myristat 13-acetate (PMA), and the calcium signaling pathway via the calcium ionophore (A23187). (unh.edu)
Similarly, the calcium ionophore A23187 evoked dopamine release from control cells, but was ineffective in stimulating dopamine release from AS1 and AS2 cells. (elsevier.com)
Effects of divalent cation ionophore A23187 on potassium permeability of rat erythrocytes. (semanticscholar.org)
The induction of eicosanoid production by lipopolysaccharide and calcium ionophore A23187 in peritoneal macrophages and furosemide-induced PGE2 excretion was also abolished in cPLA2? (immune-source.com)
It is possible that TR expression may be positively regulated by the calcium-signalling cascade, although TR induction by A23187 may be due to toxicity. (elsevier.com)
Sampaio B, Ortiz I, Resende H, Felix M, Varner DD, Hinrichs K. Factors affecting intracellular calcium influx in response to calcium ionophore A23187 in equine sperm. (msdvetmanual.com)
Variations in structural and functional changes of stallion spermatozoa in response to calcium ionophore A23187. (newhopefertility.com)
A23187 is a calcium ionophore and a microbial metabolite that has been found in S. chartreusensis. (bertin-bioreagent.com)
To stimulate the acrosome response capacitated sperm were treated with 3 μM calcium ionophore A23187 for 30 min. (conferencedequebec.org)
Vascular efects induced by calcium ionophore A23187 in renal hypertensive rat ao. (usp.br)
Endothelium-dependent relaxation was then assessed by cumulative addition of acetylcholine (ACh) or the calcium ionophore A23187. (uab.edu)
was activated with a calcium mineral ionophore considerably, "type":"entrez-nucleotide","attrs":"text":"A23187″,"term_id":"833253″,"term_text":"A23187″A23187, but inhibited by phorbol-12-myristate-13-acetate, methylamine and monensin. (complextraitgenomics.com)
MNS inhibits platelet aggregation caused by either the calcium ionophore A23187 (1 μM) or the protein kinase C (PKC) activator PDBu (200 nM) with IC50 of 25.9 μM and 4.8 μM, respectively. (invivochem.com)
The ATP hydrolysis activity of the purified (Ca 2+ + Mg 2+ )-ATPase or the SR vesicles, in the presence of the Ca 2+ ionophore A23187, is not inhibited by T 3 or T 4 . (bgu.ac.il)
The ATP hydrolysis activity of the purified (Ca2+ + Mg2+)-ATPase or the SR vesicles, in the presence of the Ca2+ ionophore A23187, is not inhibited by T3 or T4. (bgu.ac.il)
The divalent cation ionophore A23187 was used in media containing 1.75-2.89 mM calcium to challenge explants with increased intracellular calcium . (musc.edu)

For example, suboptimal concentrations of ionophores such as ionomycin exhibit slower Ca2+ efflux, which nevertheless ultimately produces the same net release as maximally effective concentrations (8). (edu.au)
2. We have studied potassium channels in human platelets by measuring the efflux of 86Rb+ (a marker for K+) from 86Rb(+)-loaded cells, and have defined their responses to stimulation by the platelet agonist thrombin and the calcium ionophore ionomycin. (ox.ac.uk)
Some assisted activation treatments such as Ca2+ (calcium)-ionophore, strontium chloride (vzorec) and ionomycin, promote an increase in intracellular free calcium concentrations by the release of calcium from cytoplasmic stores. (fertilitypedia.org)
Treatment with the calcium ionophore ionomycin induced cell death and neurite degeneration in a concentration- and time-dependent manner. (elsevier.com)
Treatment with a low concentration of ionomycin immediately produced a significant calcium influx into the intracellular region in N1E-115 cells. (elsevier.com)
These results indicate that ionomycin induces calcium influx into the intracellular region and reactive oxygen species production in N1E-115 cells. (elsevier.com)
A similar alkalinization response was produced by the calcium ionophore, ionomycin and by the calcium ATPase inhibitor, thapsigargin. (umn.edu)
Finally, the ionophore ionomycin and the Ca2+ ATPase blocker thapsigargin induce the same age related effect on beta-endorphin release. (omeka.net)
Synaptic vesicles isolated from sheep brain cortex exhibit an ATP-dependent Ca2+ accumulation that is inhibited by the protonophore uncoupler carbonyl cyanide m-chorophenylhydrazone (CCCP) and completely released by the Ca2+ ionophore ionomycin. (uc.pt)
Ionomycin is an ionophore, a compound that binds calcium ions reversibly, and is used to transport calcium ions across the cell's plasma membrane, increasing cellular calcium concentration. (smpdb.ca)
Phorbal esters, in conjunction with ionomycin, are used in this case to study the cellular response to DAG and calcium signalling without needing the cells to undergo other stimulation, mimicking things like T-cell activation easily. (smpdb.ca)

Basal levels of intracellular calcium were elevated 2- to 3-fold, intramitochondrial calcium levels were greatly increased, and mitochondrial transmembrane potential was decreased in EtBr-treated neurons. (elsevier.com)
Confocal images of intracellular calcium levels and mitochondrial localization in the same cells revealed congregations of mitochondria at the base of the axon associated with local reductions of intracellular calcium levels. (elsevier.com)
Intracellular calcium was monitored by fluorescence spectrophotometry. (elifesciences.org)
This loss of physical interaction with HSPCs could be partially restored by treating the MSCs with calcium ionophore or calmodulin, suggesting that NF-κB regulates intracellular calcium flux in the MSCs. (therapeuticsolutionsint.com)
IP3 then activates an IP3 sensitive calcium channel, increasing the concentration of intracellular calcium. (smpdb.ca)
These findings demonstrate that increased intracellular calcium produces necrotic changes in spinal cord tissue much more rapidly than increased extracellular calcium . (musc.edu)

Following stimulation of murine splenocytes with phorbol ester and calcium ionophore, we observed differences in expression of CD69, major histocompatibility complex class II molecules, the glucocorticoid-induced TNF receptor family-related gene product, and surface immunoglobulin M and D that were subsequently confirmed by Western blot or flow cytometric analysis. (ox.ac.uk)
A phorbol ester and calcium ionophore regulate sex steroid and prostaglandin release by follicles of the anuran Rana esculenta and the urodele Triturus carnifex. (unicam.it)
The degranulation of mast cells may also be induced with the artificial A-770041 substance 48/80, phorbol 12-myristate 13-acetate (PMA), and calcium mineral ionophore. (cancer-basics.com)
Andrews DA, Yang L, Low PS: Phorbol ester stimulates a protein kinase C-mediated agatoxin-TK-sensitive calcium permeability pathway in human red blood cells. (smpdb.ca)

In vitro binding studies have demonstrated that zonisamide binds to the GABA/benzodiazepine receptor ionophore complex in an allosteric fashion which does not produce changes in chloride flux. (nih.gov)
In vitro analyses of the Y152C mutation indicated a pathology similar to other known mutations in KCNJ5, including change in conductance to Na+ ions and elevated calcium levels, and increase in CYP11B2 mRNA and aldosterone production. (openrepository.com)
To further evaluate the direct effects of calcium on spinal cord tissue , without consideration of the vasculature an in vitro system was employed . (musc.edu)
These calcium-induced changes were qualitatively similar to those observed following trauma in vitro . (musc.edu)
These findings indicate that calcium-induced toxicity in vitro can be prevented to a significant degree with possible therapeutic applications following spinal cord trauma in vivo . (musc.edu)
Interestingly, transient treatments with either Ca 2+ ionophore, starvation, or a combination of both restore the fertilization capacity of C2CD6-deficient sperm in vitro. (biorxiv.org)

Apart from structural changes and meiosis resumption, there are basically two main events that characterize oocyte activation: the calcium release and the electrical events. (fertilitypedia.org)
The elevation of free intracellular (inside the cell) calcium is a nearly universal signal that triggers the cascade of events that leads to oocyte activation. (fertilitypedia.org)
The aim of artificial oocyte activation is to mimic physiological mechanisms, based mainly on calcium changes. (fertilitypedia.org)
The massive increase in cytosolic calcium occurs in periodic oscillations with amplitude and frequency that are crucial for the success of oocyte activation and embryo development. (fertilitypedia.org)
Calcium oscillations cease when pronuclei are formed, which is considered to be the end point of oocyte activation. (fertilitypedia.org)

21) Meiri H, Erulkar SD, Lerman T, Rahamimoff R. The action of the sodium ionophore, monensin, on transmitter release at the frog neuromuscular junction. (libguides.com)
Ionophores, such as monensin, are included in feed mainly to increase weight gain and to prevent bovine coccidiosis ( 3 , 4 ). (cdc.gov)

Identification of a selective manganese ionophore that enables nonlethal quantification of cellular manganese. (vanderbilt.edu)
The design of Selective ion carriers have been of considerable ionophores for Li+ is a di cult problem owing to interest as tools for the analysis and separation of the fact that lithium ions are smaller than both metal ions as well as for many biological applica- sodium and potassium ions but are strongly tions. (healthdrugpdf.com)
We have previously reported the synthesis and X-ray struc- Acyclic Li+ ionophores 1±3, which are good tural characterization of ACIA 9 carrying a neutral carriers in Li+-selective electrodes, may monoazacrown moiety [14]. (healthdrugpdf.com)
C2CD6 interacts with the CatSper calcium-selective core forming subunits. (biorxiv.org)

Deficient granulocytes had significantly increased numbers of Mo1 antigen expressed on the surface following stimulation with calcium ionophore (1 microM). (amsterdamumc.org)

Calcium chloride is added to facilitated the influx via of calcium cations. (wikipedia.org)
Others such as electrical stimulus promote influx of calcium from the extracellular medium and some treatments such as ethanol promote both effects. (fertilitypedia.org)
Calcium influx into the intracellular region is a possible activator of neurite degeneration. (elsevier.com)
Calcium influx caused by the CatSper activation results in powerful asymmetrical flagellar beating movement known as hyperactivation. (biorxiv.org)

Similar to results observed with OVCAR-8 cells, calcium treatment and STAT3 knock-down in two other ovarian cancer cell lines, OVCAR-3 and SK-OV-3 cells, resulted in increased CCN1 expression. (unh.edu)
In the presence of extracellular calcium, a depolarizing concentration of K + (56 mM) evoked dopamine release from control cells, but not from AS1 and AS2 cells. (elsevier.com)
Because the expression of antisense GAP‐43 RNA in PC12 cells leads to a decrease in GAP‐43 expression and to the loss of evoked dopamine release, these results provide evidence of a role for GAP‐43 in calcium‐dependent neurotransmitter release. (elsevier.com)
Transport via the regulated secretory pathway in semi-intact PC12 cells: role of intra-cisternal calcium and pH in the transport and sorting of secretogranin II. (rupress.org)
20152,35484} It induces calcium overload and mitochondrial apoptosis in HEK293 cells when used at a concentration of 5 µM. (bertin-bioreagent.com)
Pilot data including treatment of primary cells with calcium ionophores indicated that ZF/ZR cells may have a phenotype that is 'muted' for calcium mediated pathways. (openrepository.com)
In tumour cells, U90 is located principally in the plasma membrane fraction and cannot be induced by heat shock, glucose starvation, or treatment with tunicamycin or calcium ionophore. (microbiologyresearch.org)
Mast cells are turned on by the procedure of degranulation, which sets off the discharge of mediators such as for example histamine by calcium mineral signaling. (cancer-basics.com)
The effects of a conjugated linoleic acid (CLA) mixture of single isomers (50:50, w/w, cis9,trans11:trans10,cis12) and the individual isomers on (a) the production of resting and calcium ionophore stimulated 14C-eicosanoids and (b) the incorporation of 14C-arachidonic acid (AA) into membrane phospholipids of human saphenous vein endothelial cells were investigated. (brad.ac.uk)
Shikonin is shown to shuttle glucose into muscle cells by directly increasing the calcium concentration in skeletal muscle tissue , mimicking muscle contraction. (priceplow.com)
For these reasons, we suggest that calcium homeostasis might be important for the differences observed in the release of the opioid from cells obtained from younger ( or = 45 years) volunteers. (omeka.net)
These findings indicate calcium ions induce increased vesicular transport by the endothelial cells of the blood-spinal cord barrier indicating a pathway by which calcium ions alter the extracellular milieu of axons and glial cells . (musc.edu)
We further discovered that the ionophore treatment improved the active type of the tiny GTPase Rac1 in these cells. (cisplatin.info)
b) Snapshots from time-lapse calcium mineral imaging of one cells. (cisplatin.info)

Activation of MAP kinases by calcium-dependent and calcium-independent pathways. (elsevier.com)
Their activation leads to calcium release. (fertilitypedia.org)
The activation of MAP kinase in human being neutrophils stimulated by both uncoated and plasma-opsonized crystals of triclinic calcium pyrophosphate dihydrate (CPPD) was investigated. (mg-132.net)

Oxcarbazepine's anticonvulsant effect may occur by affecting potassium conductance and high-voltage activated calcium channels. (medscape.com)
Phosphatidylserine exposure and calcium-activated potassium efflux in platelets. (harvard.edu)
Pharmacological evidence of calcium-activated and voltage-gated potassium channels in human platelets. (ox.ac.uk)
Vesicles were loaded with 45 Ca 2+ and membrane potentials were generated by establishing potassium gradients across the vesicle using the ionophore valinomycin. (elsevier.com)
The presence of calcium ion increased the ¯uorescence slightly, but potassium and sodium had no e ect. (healthdrugpdf.com)

Interaction of charged and uncharged calcium channel antagonists with phospholipid membranes : binding equilibrium, binding enthalpy, and membrane location. (unibas.ch)
Oz, M, Tchugunova, Y & Dinc, M 2004, ' Differential effects of endogenous and synthetic cannabinoids on voltage-dependent calcium fluxes in rabbit T-tubule membranes: Comparison with fatty acids ', European Journal of Pharmacology , vol. 502, no. 1-2, pp. 47-58. (elsevier.com)
The treatment of BAECs with 30 bcg/mL cholesterol for 24 hours resulted in significant rises in total eNOS protein expression, eNOS associated with caveolae-enriched membranes, and calcium ionophore-stimulated NO production boosted by calcium ionophore-stimulated NO production. (plex.page)
The multi-subunit CatSper complexes form highly organized calcium signaling nanodomains on flagellar membranes. (biorxiv.org)

Calcium ionophores are also ideal to induce capacitation. (wikipedia.org)

We show that PMA, C. albicans and GBS use a related pathway for NET induction, whereas ionophores require an alternative pathway but that NETs produced by all stimuli are proteolytically active, kill bacteria and composed mainly of chromosomal DNA. (elifesciences.org)
In this pathway, they are shown to activate protein kinase C in the same way as calcium does, as well as mimicking the action of DG. (smpdb.ca)

28) Rahamimoff R, Erulkar SD, Alnaes E, Meiri H, Rotshenker S, Rahamimoff H. Modulation of transmitter release by calcium ions and nerve impulses. (libguides.com)

Crude chloroform extracts of fresh feverfew leaves (rich in sesquiterpene lactones) and of commercially available powdered leaves (lactone-free) produced dose-dependent inhibition of the generation of thromboxane B2 (TXB2) and leukotriene B4 (LTB4) by ionophore- and chemoattractant-stimulated rat peritoneal leukocytes and human polymorphonuclear leukocytes. (henriettesherbal.com)

Testisin localization, before and after exposure of stallion spermatozoa to capacitating conditions and calcium ionophore, was examined using live cell immunofluorescence and flow cytometry. (edu.au)

Detection and quantitation of a variety of ionophore compounds by liquid chromatography / mass spectrometry. (tamu.edu)
Quantitation of surface Mo1 on granulocytes exposed to calcium ionophore (1 microM) showed that both parents in one family but only the mother in the other family had significantly reduced levels of Mo1, suggesting heterogeneity in the inheritance of this disorder. (amsterdamumc.org)

Experimental explants were challenged with ten times the normal concentrations of extracellular calcium (17.5-29.0 mM) . (musc.edu)

Exposure of neurons to a calcium ionophore prevented axonogenesis. (elsevier.com)
Double exposure to calcium ionophore may improve blastocyst development and clinical pregnancy in some patients. (planer.com)
The calcium ionophore A23157 increased TR expression in HUVECs after a 12-h exposure, but the maximal effect was only observed after a 35-h exposure. (elsevier.com)
Zinc, selenium, vitamin D, and Zinc Ionophore supplementation via dietary supplements and/or food sources (oyster, liver…), sunshine (UVB) exposure are more important than ever to have dialed-in. (advancedhealing.com)

Here, we show that the BRAG1 IQ motif binds apo-calmodulin (CaM), and that calcium-induced CaM release triggers a reversible conformational change in human BRAG1. (jneurosci.org)

The specific objectives were (1) to determine the regulation of CCN1 expression by prostaglandin-F2α (PGF2α), signal transducer and activator of transcription 3 (STAT3) and calcium, and (2) to identify the respective cellular signaling molecules that played a role in regulating CCN1 expression. (unh.edu)

Disruption of organelle pH gradients with an ionophore or a weak base resulted in the inhibition of transport via both the constitutive and the regulated pathways. (rupress.org)
Ionophores, known by the brand names Rumensin and Bovatec, increase cell acidity and growth inhibition. (americandairymen.com)

A balanced mineral program helps reduce the risk of urinary calculi by providing the correct calcium:phosphorus ratio. (kissimmeevalleyfeed.com)
d) Histogram from the calcium mineral transient length of time. (cisplatin.info)

In contrast, the activity of the H+-ATPase, measured under uncoupling conditions (presence of CCCP), appears to be unaltered by the calcium ion. (uc.pt)

Leukotriene B 4 (LTB 4 ) concentration was determined in gastric mucosa and in whole blood after ex vivo stimulation with a calcium ionophore. (avma.org)

Zonisamide may produce these effects through action at sodium and calcium channels. (nih.gov)
The first calcium rise may be followed by an entry of calcium across the plasma membrane through voltage-sensitive calcium channels that respond to membrane depolarization. (fertilitypedia.org)
These results indicate that endocannabinoids, but not synthetic cannabinoids, directly inhibit the function of voltage-dependent calcium channels (VDCCs) and modulate the specific binding of calcium channel ligands of the dihydropyridine (DHP) class. (elsevier.com)

We also demonstrated that inhibiting the intracellular Ca2+ retarded the gastrulation cell motions considerably, while raising the intracellular Ca2+ with an ionophore improved the migration. (cisplatin.info)

C2CD6 contains a calcium-dependent membrane targeting C2 domain. (biorxiv.org)
We postulate that C2CD6 may regulate CatSper assembly, target the CatSper complex to flagellar plasma membrane, and function as a calcium sensor. (biorxiv.org)

The calcium can activate protein-tyrosine kinase 2-beta, and can also activate protein kinase C. DG also works to activate protein kinase C in the cell. (smpdb.ca)

These compounds as well as PAF produce significant increases in cytoplasmic calcium ([Ca 2+ ] i ). (umn.edu)

We studied NET production in neutrophils from healthy donors with inhibitors of molecules crucial to PMA-induced NETs including protein kinase C, calcium, reactive oxygen species, the enzymes myeloperoxidase (MPO) and neutrophil elastase. (elifesciences.org)

Elevated total serum calcium levels are associated with idiopathic hypercalcemia, vitamin D intoxication, hyperparathyroidism, sarcoidosis, pneumocystic carinii pneumonia, and blue diaper syndrome. (cdc.gov)

Calcium ionophore treatment is now the only commonly used method of AOA. (fertilitypedia.org)

When taken together with previous data indicating important roles for calcium in regulating neurite outgrowth, the present findings suggest critical roles for mitochondrial function and modulation of calcium homeostasis in the establishment of neuronal polarity. (elsevier.com)
Functional cell-based assays often monitor for changes in neuronal activity with calcium imaging, patch clamp electrophysiology, or label-free microelectrode array (MEA) recordings . (axionbiosystems.com)

Vitamin D is an important hormone and plays critical roles in the regulation of calcium and phosphate metabolism. (medium.com)
Role of calcium in heart metabolism. (nih.gov)

We also demonstrate calcium channel blocker, verapamil as an efficient blocker of mKCNJ5 mediated aldosterone production. (openrepository.com)

In D. G. Lambert (Ed.), Calcium Signaling Protocols (Vol. 114, pp. 235-248). (edu.au)

We have previously shown that volatile anesthetics inhibit glutamate-stimulated [ 3 H]MK-801 binding to the ionophore of NMDA receptor complexes in rat brain. (elsevier.com)

Anatomy5

Organisms3

Chemicals and Drugs73

Analytical, Diagnostic and Therapeutic Techniques and Equipment3

Phenomena and Processes11

Technology, Industry, Agriculture2

Changes in content and localization of proteins phosphorylated at tyrosine, serine and threonine residues during ram sperm capacitation and acrosome reaction. (1/68)

The use of anti-VDAC2 antibody for the combined assessment of human sperm acrosome integrity and ionophore A23187-induced acrosome reaction. (2/68)

Ultrastructure and intracellular calcium response during activation in vitrified and slow-frozen human oocytes. (3/68)

Effects of FK506 and cyclosporin a on calcium ionophore-induced mitochondrial depolarization and cytosolic calcium in astrocytes and neurons. (4/68)

Calpain-cleaved type 1 inositol 1,4,5-trisphosphate receptor (InsP(3)R1) has InsP(3)-independent gating and disrupts intracellular Ca(2+) homeostasis. (5/68)

Activation of human vascular cells decreases their expression of transforming growth factor-beta. (6/68)

Chromatin disruption in the promoter of bovine leukemia virus during transcriptional activation. (7/68)

The redox state of transglutaminase 2 controls arterial remodeling. (8/68)

Ionophores

Lasalocid

Calcimycin

Ionomycin

Ethers

Calcium Ionophores

Calcium

Egtazic Acid

Tetradecanoylphorbol Acetate

Calcium Signaling

Nigericin

Monensin

Cells, Cultured

Valinomycin

Calcium Channels

Calcium, Dietary

Potassium Ionophores

Calcium Carbonate

Carbonyl Cyanide m-Chlorophenyl Hydrazone

Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone

Sodium Ionophores

Calcium Phosphates

Calcium Isotopes

Calcium Chloride

Gramicidin

Hydrogen-Ion Concentration

Kinetics

Potassium

Membrane Potentials

Calcium Channels, L-Type

Biological Transport, Active

Furans

Calcium Oxalate

Butylamines

Calcium Gluconate

Sodium

Biological Transport

Calcium Radioisotopes

Adenosine Triphosphate

Lanthanum

Magnesium

Ion-Selective Electrodes

Arsenazo III

Proton Ionophores

Potentiometry

Thapsigargin

Cattle

Verapamil

Methylamines

Calcium Compounds

Chelating Agents

Calcium Channels, N-Type

Ruminants

2,4-Dinitrophenol

Veterinary Drugs

Dicyclohexylcarbodiimide

Cytosol

Protons

Salicylanilides

Calcium Channel Agonists

Coccidiostats

Anti-Bacterial Agents

Time Factors

Cations, Divalent

Fluorescent Dyes

Terpenes

Ammonium Chloride

Aminoquinolines

Cell Line

Cell Membrane Permeability

Dose-Response Relationship, Drug

Uncoupling Agents

Rabbits

Potassium Cyanide

Edetic Acid

Cations

Calcium Hydroxide

Liposomes

Phosphorus

Rumen