Chemical agents that increase the permeability of biological or artificial lipid membranes to specific ions. Most ionophores are relatively small organic molecules that act as mobile carriers within membranes or coalesce to form ion permeable channels across membranes. Many are antibiotics, and many act as uncoupling agents by short-circuiting the proton gradient across mitochondrial membranes.
An ionophorous, polyether antibiotic from Streptomyces chartreusensis. It binds and transports CALCIUM and other divalent cations across membranes and uncouples oxidative phosphorylation while inhibiting ATPase of rat liver mitochondria. The substance is used mostly as a biochemical tool to study the role of divalent cations in various biological systems.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Signal transduction mechanisms whereby calcium mobilization (from outside the cell or from intracellular storage pools) to the cytoplasm is triggered by external stimuli. Calcium signals are often seen to propagate as waves, oscillations, spikes, sparks, or puffs. The calcium acts as an intracellular messenger by activating calcium-responsive proteins.
An antiprotozoal agent produced by Streptomyces cinnamonensis. It exerts its effect during the development of first-generation trophozoites into first-generation schizonts within the intestinal epithelial cells. It does not interfere with hosts' development of acquired immunity to the majority of coccidial species. Monensin is a sodium and proton selective ionophore and is widely used as such in biochemical studies.
A cyclododecadepsipeptide ionophore antibiotic produced by Streptomyces fulvissimus and related to the enniatins. It is composed of 3 moles each of L-valine, D-alpha-hydroxyisovaleric acid, D-valine, and L-lactic acid linked alternately to form a 36-membered ring. (From Merck Index, 11th ed) Valinomycin is a potassium selective ionophore and is commonly used as a tool in biochemical studies.
Voltage-dependent cell membrane glycoproteins selectively permeable to calcium ions. They are categorized as L-, T-, N-, P-, Q-, and R-types based on the activation and inactivation kinetics, ion specificity, and sensitivity to drugs and toxins. The L- and T-types are present throughout the cardiovascular and central nervous systems and the N-, P-, Q-, & R-types are located in neuronal tissue.
Carbonyl Cyanide m-Chlorophenyl Hydrazone
Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone
An element in the alkali group of metals with an atomic symbol K, atomic number 19, and atomic weight 39.10. It is the chief cation in the intracellular fluid of muscle and other cells. Potassium ion is a strong electrolyte that plays a significant role in the regulation of fluid volume and maintenance of the WATER-ELECTROLYTE BALANCE.
The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization).
Calcium Channels, L-Type
Long-lasting voltage-gated CALCIUM CHANNELS found in both excitable and nonexcitable tissue. They are responsible for normal myocardial and vascular smooth muscle contractility. Five subunits (alpha-1, alpha-2, beta, gamma, and delta) make up the L-type channel. The alpha-1 subunit is the binding site for calcium-based antagonists. Dihydropyridine-based calcium antagonists are used as markers for these binding sites.
Biological Transport, Active
Calcium Channels, N-Type
Drugs used by veterinarians in the treatment of animal diseases. The veterinarian's pharmacological armamentarium is the counterpart of drugs treating human diseases, with dosage and administration adjusted to the size, weight, disease, and idiosyncrasies of the species. In the United States most drugs are subject to federal regulations with special reference to the safety of drugs and residues in edible animal products.
Calcium Channel Agonists
Agents that increase calcium influx into calcium channels of excitable tissues. This causes vasoconstriction in VASCULAR SMOOTH MUSCLE and/or CARDIAC MUSCLE cells as well as stimulation of insulin release from pancreatic islets. Therefore, tissue-selective calcium agonists have the potential to combat cardiac failure and endocrinological disorders. They have been used primarily in experimental studies in cell and tissue culture.
Cell Membrane Permeability
Dose-Response Relationship, Drug
The first stomach of ruminants. It lies on the left side of the body, occupying the whole of the left side of the abdomen and even stretching across the median plane of the body to the right side. It is capacious, divided into an upper and a lower sac, each of which has a blind sac at its posterior extremity. The rumen is lined by mucous membrane containing no digestive glands, but mucus-secreting glands are present in large numbers. Coarse, partially chewed food is stored and churned in the rumen until the animal finds circumstances convenient for rumination. When this occurs, little balls of food are regurgitated through the esophagus into the mouth, and are subjected to a second more thorough mastication, swallowed, and passed on into other parts of the compound stomach. (From Black's Veterinary Dictionary, 17th ed)
A calcium salt that is used for a variety of purposes including: building materials, as a desiccant, in dentistry as an impression material, cast, or die, and in medicine for immobilizing casts and as a tablet excipient. It exists in various forms and states of hydration. Plaster of Paris is a mixture of powdered and heat-treated gypsum.
A polypeptide hormone (84 amino acid residues) secreted by the PARATHYROID GLANDS which performs the essential role of maintaining intracellular CALCIUM levels in the body. Parathyroid hormone increases intracellular calcium by promoting the release of CALCIUM from BONE, increases the intestinal absorption of calcium, increases the renal tubular reabsorption of calcium, and increases the renal excretion of phosphates.
Changes in content and localization of proteins phosphorylated at tyrosine, serine and threonine residues during ram sperm capacitation and acrosome reaction. (1/68)(+info)
The use of anti-VDAC2 antibody for the combined assessment of human sperm acrosome integrity and ionophore A23187-induced acrosome reaction. (2/68)(+info)
Ultrastructure and intracellular calcium response during activation in vitrified and slow-frozen human oocytes. (3/68)(+info)
Effects of FK506 and cyclosporin a on calcium ionophore-induced mitochondrial depolarization and cytosolic calcium in astrocytes and neurons. (4/68)(+info)
Calpain-cleaved type 1 inositol 1,4,5-trisphosphate receptor (InsP(3)R1) has InsP(3)-independent gating and disrupts intracellular Ca(2+) homeostasis. (5/68)(+info)
Activation of human vascular cells decreases their expression of transforming growth factor-beta. (6/68)(+info)
Chromatin disruption in the promoter of bovine leukemia virus during transcriptional activation. (7/68)(+info)
The redox state of transglutaminase 2 controls arterial remodeling. (8/68)(+info)
Molecules | Free Full-Text | The Ameliorative Effect of Sophoricoside on Mast Cell-Mediated Allergic Inflammation in Vivo and...
Sophoricoside exhibits numerous pharmacological effects, including anti- inflammatory and anti-cancer actions, yet the exact mechanism that accounts for the anti-allergic effects of sophoricoside is not completely understood. The aim of the present study was to elucidate whether and how sophoricoside modulates the mast cell-mediated allergic inflammation in vitro and in vivo. We investigated the pharmacological effects of sophoricoside on both compound 48/80 or histamine-induced scratching behaviors and 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis in mice. Additionally, to find a possible explanation for the anti-inflammatory effects of sophoricoside, we evaluated the effects of sophoricoside on the production of histamine and inflammatory cytokines and activation of nuclear factor-κB (NF-κB) and caspase-1 in phorbol 12-myristate 13-acetate plus calcium ionophore A23187 (PMACI)-stimulated human mast cells (HMC-1). The finding of this study demonstrated that sophoricoside reduced
Anoctamins are a family of Ca2+-activated Cl− channels | Journal of Cell Science
The data presented above indicate that Ano6, 8 and 9, when overexpressed in HEK293 cells, produce Cl− currents upon increase in intracellular Ca2+ by ionomycin. However, although all three paralogs are expressed endogenously in HEK293 cells, no currents are activated in mock-transfected cells (Fig. 3A) (Almaça et al., 2009). However, when increasing [Ca2+]i to very high levels (≥10 µM), endogenous anoctamins were activated (Fig. 3B,E). This may be due to a lack of sufficient membrane expression of endogenous anoctamins. Endogenous anoctamins are expressed at much lower levels than overexpressed proteins and may be inhibited by endogenous accessory proteins, such as CAMKII. Alternatively, specific Ca2+ release and/or influx pathways need to be triggered to activate these endogenous anoctamins. The overall linear structures appear similar in all anoctamins (supplementary material Fig. S1) and an analysis of putative functional domains revealed a number of similar motifs and putative ...
Volume regulations by Anoctamins, Bestrophin1 and Transmembrane Channel like proteins - University of Regensburg Publication...
Volumenregulation ist ein grundlegender Mechanismus, der für alle lebenden Organismen notwendig ist. Zellen schwellen unter einem hypo- osmotischen Schock und aktivieren darauf die Freisetzung von K+ und Cl- Ionen über die Aktivierung von K+- und Cl-- Kanälen, um das Ausgangsvolumen wieder herzustellen. Im Jahre 2014 wurde von zwei unabhängigen Arbeitsgruppen LRRC8A als Hauptkomponente des Volumen- Regulierten Anionen Canals (VRAC) identifiziert. Die Ergebnisse der vorliegenden Arbeit lassen aber vermuten, dass auch Anoctamine und Bestrophine an der Volumenregulation beteiligt sind. Darüber hinaus konnte für diese Proteine der Mechanismus ihrer zellvolumen-abhängigen Aktivierung geklärt werden. Zusätzlich wurde in dieser Arbeit die Rolle der TMC Proteine (TransMembrane Channel-like family of Proteins), die eine hohe Sequenzähnlichkeit zu den Anoctaminen besitzen, für die Volumenregulation und für die Regulation der intrazellulären Ca2+ Konzentration untersucht. Überexpression von ...
Histopathological assessment of the anti-osteoporosis e | Open-i
Histopathological assessment of the anti-osteoporosis effect of sophoricoside.Normal group (A) shows normal bony tissue with intact well-formed dense trabeculae
Phospholipid scramblase elisa and antibody
Shop Phospholipid scramblase ELISA Kit, Recombinant Protein and Phospholipid scramblase Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Anoctamins and Bestrophins are two kinds of calcium-activated chloride channels. Anoctamins were shown to be Ca-activated Cl channels in 2008 and are responsible for the classical CaCCs that are widely expressed in many tissues, especially epithelia. Bestrophins are more restricted in their expression and play more specialized roles. Bestrophin-1 is expressed in the retinal pigment epithelium and certain mutations in bestrophin-1 cause degeneration of the retina and blindness. Bestrophin-2 is expressed in goblet cells in colon and may play a role in bicarbonate secretion in this tissue. We are interested in understanding how both anoctamins and bestrophins are regulated by calcium. We are identifying the calcium binding sites and the molecular links between the calcium sensor and the channel gate using a combination of both whole-cell and single channel electrophysiological mesurements and mutagenesis. We are dissecting the signaling protein network associated with these channels using ...
phospholipid scramblase activity Antibodies | Invitrogen ...
Antibodies for proteins involved in phospholipid scramblase activity pathways, according to their Panther/Gene Ontology Classification
PLSCR4 elisa kit | Human Phospholipid scramblase 4 ELISA Kit-Q9NRQ2.2
Buy PLSCR4 elisa kit, Human Phospholipid scramblase 4 ELISA Kit-Q9NRQ2.2 (MBS283171) product datasheet at MyBioSource, ELISA Kits
SIRT1 suppresses PMA and ionomycin-induced ICAM-1 expression in endothelial cells
Intercellular adhesion molecule-1 (ICAM-1) plays an important role in the recruitment of leukocytes to the endothelium, which causes inflammation and initiation of atherosclerosis. We have previously shown that endothelium-specific over-expression of class III deacetylase SIRT1 decreases atheroscler …
PLSCR1 phospholipid scramblase 1 [Homo sapiens (human)] - Gene - NCBI
These reference sequences exist independently of genome builds. Explain. These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above. ...
DPY19L1 antibody | acris-antibodies.com
Kit Component:- KN304792G1, Dpy19l1 gRNA vector 1 in pCas-Guide vector- KN304792G2, Dpy19l1 gRNA vector 2 in pCas-Guide vector- KN304792D, donor…
PLSCR3 (phospholipid scramblase 3 Scr3) is one of the superfamily of - Small Molecule Inhibitors of Protein Arginine...
PLSCR3 (phospholipid scramblase 3 Scr3) is one of the superfamily of membrane-associated transcription regulators named Tubby-like protein (TULPs). medium LAQ824 by means of extracellular microvesicles (exosomes). Alternatively Scr3 expression didnt decrease as well as the secretion of Scr3 significantly?in 3T3 Swiss-albino fibroblasts (a parental cell-line of 3T3-L1) had not been increased by differentiation treatment. Overexpression of human being Scr3 during 3T3-L1 differentiation suppressed triacylglycerol build up and inhibited induction from the mRNAs lately stage pro-adipogenic transcription elements [CCAAT/enhancer-binding proteins α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ)] and X-box-binding proteins 1 (XBP1). Manifestation of early stage pro-adipogenic transcription elements (C/EBPβ and C/EBPδ) had not been considerably affected. These outcomes claim that Scr3 features as a poor regulator of adipogenesis in 3T3-L1 cells at a particular differentiation ...
PLSCR1 - Phospholipid scramblase - Homo sapiens (Human) - PLSCR1 gene & protein
May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane.
Sequential shrinkage and swelling underlie P2X(7)-stimulated lymphocyte phosphatidylserine exposure and death - Surrey...
Taylor, SRJ, Gonzalez-Begne, M, Dewhurst, S, Chimini, G, Higgins, CF, Melvin, JE and Elliott, JI (2008) Sequential shrinkage and swelling underlie P2X(7)-stimulated lymphocyte phosphatidylserine exposure and death ...
Aspirin-sensitive asthma: COX-2 inhibitors may be safe | APhA DrugInfoLine
APhA DrugInfoLine (ISSN 2162-3015) is a weekly publication of, and is owned and copyrighted by the American Pharmacists Association, the national professional society of pharmacists. Materials in APhA DrugInfoLine do not neccessarily represent the policy, recommendations, or endorsement of APhA. The publisher, authors, editors, reviewers, and contributors have taken care to ensure that the information contained in APhA DrugInfoLine is accurate and current; however, they shall have no ability to any person or entity with regard to claims, losses, or damage caused or alleged to be caused, directly or indirectly by use of any information contained in the publication. All decisions about drug therapy must be based on independent judgement of the clinician.. ...
Ano ano ba ang mga gamot na pampatigas - Revitol Skin Care - Oct 10, 2017
Ano ano ba ang mga gamot na pampatigas - inda dadaesen Ang Lungsod - W2C - Web To Corpus. Ideal for treating acne spots directly.
Tmem18兔多克隆抗体(ab106597)可与小鼠, 大鼠, 人样本反应并经WB, IHC, ICC/IF实验严格验证。所有产品均提供质保服务，中国75%以上现货。
Expression of interleukin-5 and granulocyte-macrophage colony-stimulating factor in aspirin-sensitive and non-aspirin-sensitive...
Increased numbers of eosinophils and mast cells in the bronchial mucosa are characteristic features in subjects with aspirin-sensitive asthma. Interleukin-5 (IL-5) and granulocyte-macrophage colony-stimulating factor (GM-CSF) are involved in the activation, maturation, and perpetuation of survival o …
P2X7 receptor activation causes phosphatidylserine exposure in human erythrocytes
Sluyter, R., Shemon, A. & Wiley, J. S. (2007). P2X7 receptor activation causes phosphatidylserine exposure in human erythrocytes. Biochemical and Biophysical Research Communications, 355 169-173 ...
Babi nazna il, e Falt nek ji nebude obhajovat post v ele ANO. Prvn m stop edseda hnut to zat m nepotvrdil | Domov |...
P edseda poslaneck ho klubu ANO Jaroslav Falt nek z ejm p t rok nebude na sn mu obhajovat funkci prvn ho m stop edsedy hnut . Po sch zce s n m to televizi Nova ekl p edseda hnut a premi r Andrej Babi . D vodem je z ejm situace kolem brn nsk organizace ANO, kterou Falt nek zakl dal.
TMEM204兔多克隆抗体(ab113102)可与小鼠, 大鼠, 人样本反应并经WB, IHC, ICC, ICC/IF实验严格验证。所有产品均提供质保服务，中国75%以上现货。
Constitutive Nuclear Factor κB Activity Is Required for Survival of Activated B Cell-like Diffuse Large B Cell Lymphoma Cells ...
This conclusion was supported by studies of IκBα protein turnover in the DLBCL cell lines. Because IκBα transcription is itself positively regulated by NF-κB activity (37, 38), the steady-state level of IκBα protein does not necessarily reflect the rate of phosphorylation by IKK and subsequent degradation. Indeed, full-length IκBα protein is found at comparable levels in our untreated cell lines (Fig. 3 A). However, when ABC DLBCL cell lines were treated with cycloheximide (CHX) to block new protein synthesis, the level of IκBα declined rapidly through phosphorylation and degradation (Fig. 3 A). By contrast, the IκBα level in SUDHL-6 was significantly more stable upon CHX treatment unless the cells were also stimulated by BCR cross-linking or by PMA and ionomycin treatment (Fig. 3 A). Lactacystin, a drug that inhibits degradation of ubiquitinated IκBα by proteasomes, prevented the CHX-induced loss of IκBα in resting ABC DLBCL cell lines and in stimulated SUDHL-6 cells (Fig. 3 ...
Phospholipid scramblase - Anolis carolinensis (Green anole)
May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane.
T cell self-reactivity forms a cytokine milieu for spontaneous development of IL-17+ Th cells that cause autoimmune arthritis |...
In vitro PMA/ionomycin treatment for 5 h, which activates a signal transduction step down-stream of ZAP-70 and therefore equally activates SKG and BALB/c T cells, revealed that a significant fraction of LN CD4+ T cells from nonarthritic SKG mice in an SPF environment were producing IL-17A (hereafter IL-17), whereas SKG or BALB/c CD4-SP thymocytes or BALB/c CD4+ T cells were not (Fig. 1 A and Fig. S1, which is available at http://www.jem.org/cgi/content/full/jem.20062259/DC1). Such IL-17-producing SKG CD4+ T cells also produced at a single cell level TNF-α and IL-2, but not IFN-γ, IL-4, or IL-10, a profile distinct from Th1 or Th2 cells and similar to that of Th17 cells (Fig. 1 A; references 7-9). CD4+ T cells freshly prepared from nonarthritic SKG mice also actively transcribed IL-17 and IL-23R mRNA (Fig. 1 B). In arthritic SKG mice raised in a conventional environment, arthritic joints actively transcribed IL-17 mRNA, whereas nonarthritic ones did not (Fig. 1 C). Correspondingly, CD4+ T cells ...
Infection Ano MM2k9 EMag
w00t! after 6 long weeks my emag has returned from ano! I could not be happier with the finish :D Enough chit-chat... PICTURES! Before: http://i1046.photobucket.com/albums/b461/sQuid-Vision/0F07329E-3CBC-41F4-B2CF-A4E8531CA79B-7719-0000081BE189A8F7.jpg Broken Down for Ano:
LQES - Publicações e Teses - Comunicações em Congressos Internacionais - 2009
COMUNICAÇÕES APRESENTADAS EM CONGRESSOS INTERNACIONAIS As Comunicações estão organizadas em duas partes. A primeira compreende o período de 1976-1995 e, no segundo, temos informações apresentadas, ano a ano, a partir de 1996.. ...
Most recent papers with the keyword phospholamban | Read by QxMD
Unregulated increases in cellular Ca2+ homeostasis are a hallmark of pathophysiological conditions and a key trigger of cell death. Endothelial cells cultured under physiologic O2 conditions (5% O2) exhibit a reduced cytosolic Ca2+ response to stimulation. The mechanism for reduced plateau [Ca2+]i upon stimulation was due to increased sarco/endoplasmic reticulum Ca2+ ATPase (SERCA)-mediated reuptake rather than changes in Ca2+ influx capacity. Agonist-stimulated phosphorylation of the SERCA regulatory protein phospholamban was increased in cells cultured under 5% O2 Elevation of cytosolic and mitochondrial [Ca2+] and cell death after prolonged ionomycin treatment, as a model of Ca2+ overload, were lower when cells were cultured long-term under 5% compared with 18% O2 This protection was abolished by cotreatment with the SERCA inhibitor cyclopiazonic acid ...
Phosphatidylserine and phospholipid scramblase in mast cell signaling | CU Digital Repository
6. CONCLUSIONS 1. We found that mast cell stimulation can induce PS externalization in the absence of secretory response. 2. We identified GPI-APs as molecules whose engagement can induce sustained and reversible non-apoptotic PS externalization. 3. GPI-AP-induced PS externalization was determined as non- apoptotic and distinct from the FceRl-induced PS externalization. 4. The effect of multiple triggering on PS externalization was additive and dependent on a tlpe of stimulus and cells engaged. 5. We identifred PLSCR1 as a molecule that becomes tyrosine phosphorylated in mast cells stimulated through GPI-APs. 6. We found that the PLSCR1 tyrosine phosphorylation is not associated with mast cell secretory response' and with GPI-AP- or FceRl-induced non-apoptotic PS externalization. 7. Using confocal microscopy and electron microscopy visualization of PLSCR1 in the course of mast cell activation we found that PLSCR1: (1) is not co-localized with extemalized PS, (2) is not co- localized with ...
Relief for Aspirin-Sensitive Asthma Patients | Division of Allergy & Immunology | Albert Einstein College of Medicine
The lack of understanding of AERD and its underlying cause has created a critical barrier in developing effective treatments for the condition. Many practitioners are unaware of AERD and this is often why it takes so long to connect all the pieces of the puzzle before the correct treatment can be offered, Dr. Jerschow said.. Additionally, the very act of diagnosing AERD is risky, since many patients will suffer an asthma attack in response to the standard oral-graded aspirin challenge. Dr. Jerschows study will compare the pharmacological effects (biochemical changes in the body) of aspirin in AERD patients to those of aspirin-tolerant asthmatics. Her protocol, conducted on 25-30 participants aged 25-62 (some of whom are referred by the Department of Otorhinolaryngology - Head & Neck Surgery), will use a low-dose aspirin challenge to avoid allergic reactions. The low-dose aspirin challenge begins by giving the patient a small amount of aspirin. This approach usually does not lead to a ...
Recombinant Human DPY30 protein (ab135020) | Abcam
Recombinant Human DPY30 protein is an Escherichia coli Full length protein 2 to 99 aa range, |=85% purity and validated in SDS-PAGE.
Single-Cell Mass Cytometry of Differential Immune and Drug Responses Across a Human Hematopoietic Continuum | Science
Effect of pharmacologic kinase inhibition on normal hematopoietic signaling. Having established a baseline of healthy signaling responses to a panel of stimuli, we examined cell type-specific pharmacologic effects of some well-characterized kinase inhibitors. These included the Janus kinase (JAK) I inhibitor and MAPK kinase (MEK) inhibitor U0126. Predictably, when combined respectively with G-CSF and PMA/Ionomycin treatments of human BM (figs. S8 and S9) reliable and specific inhibition, respectively, of STAT3 and ERK1/2 phosphorylation are observed, which is consistent with previously reported observations that used conventional single-cell analysis platforms (44). Although interesting results were obtained with these inhibitors, we expanded to focus on dasatinib, a clinically relevant small-molecule kinase inhibitor. Dasatinib was originally introduced as a second-line BCR-ABL kinase inhibitor for imatinib-resistant chronic myelogenous leukemia (CML) (45). Unlike imatinib, dasatinib is ...
Bisphosphonates as Potential Drug Candidates for Inflammatory Lung Diseases
Besides their use in inflammatory lung diseases the novel bisphosphonates with prolonged carbon chain may be used as treatment option for cystic fibrosis and atherosclerosis.,br,,br, ,b,Benefits:,/b,,br, ,ul, ,li,Neutral sphingomyelinase is not influenced,/li, ,li,First ex-vivo data in a PAF-induced edema rat model showed clear edema reduction by 50percent,/li, ,li,Inhibition of dexamethasone induced apoptosis was proven ,em,in vitro,/em,,/li, ,li,Substances can easily be synthesized in a one-step or two-step procedure,/li, ,li,Prolongation from 6 to 8 C-atoms shows 40times higher inhibition rate,/li, ,/ul, ,p,,strong,IP Rights,/strong,,br, EP Application (08/2009).,br, PCT Application (08/2010). ,br, A PCT application was filed in August 2010.,br, ,br, ,strong,Patent Owner,/strong,,br, Humboldt-Universität zu Berlin,br, Rheinisch-Westfälische,br, Technische Hochschule Aachen (RWTH),br,,br, ,b,Find the technology offer and contact details here: ,a ...
Ano ang Latter Rain Movement?
Ano ang Latter Rain Movement? Magkakaroon ba ng pagbubuhos ng mga mahimalang espiritwal na kaloob habang nalalapit ang mga huling araw?
Calcium ionophores are also ideal to induce capacitation. Adding heparin to capacitation inducing medium mimics the secretion ... Calcium chloride is added to facilitated the influx via of calcium cations. In animal models, Tyrode's albumin lactate pyruvate ...
Fenstein, MB; Fraser, C (1975). "Human platelet secretion and aggregation induced by calcium ionophores. Inhibition by PGE1 and ... IP receptors bind with ionophores that induce ADP and serotonin secretion. PGE1 inhibits the secretion of factors that ... 5-triphosphate signaling and the mobilization of calcium inside the cell for thromboxane A2. PGI2, prostacyclin, binds to IP ...
... however others have found it necessary to also use calcium ionophore treatment for fertilisation to be successful. Calcium ... "Mechanism of action of calcium ionophores on intact cells: ionophore-resistant cells". Membrane & Cell Biology. 13 (3): 357-368 ... It has been found that treating globozoospermia with ICSI along with oocyte activation by calcium ionophore (an ion carrier ... "A Successful Healthy Childbirth in a Case of Total Globozoospermia with Oocyte Activation by Calcium Ionophore". Journal of ...
Jamil, K.; White, I. G. (December 1981). "Induction of acrosomal reaction in sperm with ionophore A23187 and calcium". Archives ... as well as the more commonly used calcium ionophore A23187. This can be done to serve as a positive control when assessing the ...
... and calcium ionophore-induced arachidonic acid release". J. Biol. Chem. 278 (40): 38206-13. doi:10.1074/jbc.M304816200. PMID ... CERK was later confirmed to bind calmodulin in the presence of calcium, indicating the calmodulin first binds calcium and then ... A calcium-stimulated lipid kinase that co-purifies with brain synaptic vesicles". J. Biol. Chem. 264 (24): 14354-60. doi: ... CERK has also been found to participate in the calcium-dependent degranulation of mast cells. Sugiura M, Kono K, Liu H, ...
Unfolded protein response
Lee AS (January 1987). "Coordinated regulation of a set of genes by glucose and calcium ionophores in mammalian cells". Trends ... This calcium then interacts with calcineurin and calcium/calmodulin-dependent kinases that in turn activate transcription ... Muscular contraction during exercise causes calcium to be released from the sarcoplasmic reticulum (SR), a specialized ER ... calcium that is stored bound to resident molecular chaperones; and redox buffers that maintain the oxidizing environment ...
Anti-citrullinated protein antibody
Asaga H, Yamada M, Senshu T (February 1998). "Selective deimination of vimentin in calcium ionophore-induced apoptosis of mouse ...
Calcium Ionophore, Antibiotic A23187 and Calcium Ionophore A23187. It is produced at fermentation of Streptomyces ... The ionophore is used in laboratories to increase intracellular Ca2+ levels in intact cells. It also uncouples oxidative ... It also acts as a divalent cation ionophore, allowing these ions to cross cell membranes, which are usually impermeable to them ... of Topotecan Enhances Anticancer Efficacy in Murine and Human Xenograft Models Cancer Research Effect of calcium ionophore on ...
ARE-directed mRNA degradation is influenced by many exogenous factors, including phorbol esters, calcium ionophores, cytokines ...
... is an ionophore and an antibiotic that binds calcium ions (Ca2+) in a ratio 1:1. It is produced by the bacterium ... Its β-diketone and carboxylic acid group form a chelate with calcium. It was extracted in 1978 and the complete structure was ... It is used in research to raise the intracellular calcium level (Ca2+) and as a research tool to understand Ca2+ transport ... 1979). "Structure of ionomycin - a novel diacidic polyether antibiotic having high affinity for calcium ions". Journal of the ...
Reiss, Hans-Dieter; Herth, Werner (1979). "Calcium ionophore A 23187 affects localized wall secretion in the tip region of ...
"Epidermal growth factor induces p11 gene and protein expression and down-regulates calcium ionophore-induced arachidonic acid ... "Epidermal growth factor induces p11 gene and protein expression and down-regulates calcium ionophore-induced arachidonic acid ... S100 calcium-binding protein A10 (S100A10), also known as p11, is a protein that is encoded by the S100A10 gene in humans and ... Wu T, Angus CW, Yao XL, Logun C, Shelhamer JH (Jul 1997). "P11, a unique member of the S100 family of calcium-binding proteins ...
... may be artificially facilitated by calcium ionophores, something that is speculated to be useful in case of ... The slow block is through a biochemical mechanism triggered by a wave of calcium increase. The rise of calcium is both ... "Roscovitine in combination with calcium ionophore induces oocyte activation through reduction of M-phase promoting factor ... Sperm entry causes calcium release into the oocyte. In mammals, this is caused by the introduction of phospholipase C isoform ...
Peter K. Hepler
Saunders, M. J.; P. K. Hepler (1982). "Calcium ionophore A23187 stimulates cytokinin-like mitosis in Funaria". Science. 217 ( ... This calcium binding may account for the bulk of the observed extracellular current. The intracellular calcium gradient may ... He has shown that calcium ions and protons are essential for growth. The intracellular free calcium ions exist in a gradient ... The delay between the extracellular and intracellular calcium peaks indicates that calcium ions do not immediately enter the ...
The anergy in T cells can be induced by Ionomycin, the ionophore capable of raising intracellular concentration of calcium ions ... Under this condition, calcium dependent phosphatase calcineurin removes phosphates from a transcriptional factor NFAT, which in ... On the contrary, TCR signalling without costimulatory receptors sufficiently activates only the calcium arm of the signalling ... calcium dependent arm of a lymphocyte signalling is activated by TCR. This leads to an elevation of intracellular Ca+II ...
Potassium selective electrode
Interference from calcium, magnesium, or lithium, on the other hand, is weak enough that their presence in normal ... Ionophores for potassium-selective electrodes v t e (Electrodes, All stub articles, Electrochemistry stubs). ... These electrodes are typical ion exchange resin membrane electrodes, using valinomycin, a potassium ionophore, as the ion ... calcium, magnesium, and lithium. The most significant interference with measurement of potassium concentration is from the ...
G-CSF factor stem-loop destabilising element
UTR of G-CSF mRNA contains a destablizing element that is insensitive to calcium ionophore, hence SLDE regulates G-CSF mRNA. ...
Sperm activation has been shown to be caused by calcium ionophores in vitro, progesterone released by nearby cumulus cells and ... The opening of CatSper channels is responsible for the influx of calcium. The sudden rise in calcium levels causes the ... A recent discovery links hyperactivation to a sudden influx of calcium ion into the tails. The whip-like tail (flagellum) of ... These channels are selective, allowing only calcium ions to pass. ...
Sperm activation has been shown to be caused by calcium ionophores in vitro, progesterone released by nearby cumulus cells and ... The opening of CatSper channels is responsible for the influx of calcium. The sudden rise in calcium levels causes the ... A recent discovery links hyperactivation to a sudden influx of calcium ion into the tails. The whip-like tail (flagellum) of ... These channels are selective, allowing only calcium ion to pass. ...
Cryptic female choice
... slow and inert in the reproductive tracts or storage areas of the female and are only activated by calcium ionophores in vitro ...
PCP-2 mRNA is regulated by phorbol myristate acetate (PMA) or calcium ionophore, okadaic acid, the Ras inhibitor manumycin, and ... down-regulation of gene expression by PMA and calcium ionophore in Jurkat T lymphoma cells". Biochem Biophys Res Commun. 231 (1 ...
... of agents such as the chelator EGTA and the ionophore A23187 could have been incorrectly said to be specific for calcium in ... The traditional supply of calcium salts to fungal cultures may have met the need for trace elements as even A.R. calcium salts ... In the experiments with oxygen and hyphal development there was no requirement for calcium and no inhibition by EGTA. At the ... time of these studies many mycologists thought that calcium played a role in fungal morphology and were disinclined to believe ...
List of MeSH codes (D27)
... calcium channel agonists MeSH D27.505.519.562.249 - calcium channel blockers MeSH D27.505.519.562.374 - ionophores MeSH D27.505 ... calcium channel agonists MeSH D27.505.954.411.793.610 - nasal decongestants MeSH D27.505.954.411.918 - vasodilator agents MeSH ... calcium channel blockers MeSH D27.505.954.411.207 - cardioplegic solutions MeSH D27.505.954.411.222 - cardiotonic agents MeSH ...
COAT platelet defect
... scrambling and do not express negatively charged phospholipids on their surface even after treatment with calcium ionophores. ...
... down-regulation of gene expression by PMA and calcium ionophore in Jurkat T lymphoma cells". Biochemical and Biophysical ... Flentke GR, Garic A, Amberger E, Hernandez M, Smith SM (July 2011). "Calcium-mediated repression of β-catenin and its ... Soluble inositol-(1,4,5)-trisphosphate triggers calcium to be released from the endoplasmic reticulum. This sudden increase in ... "Modification of the composition of polycystin-1 multiprotein complexes by calcium and tyrosine phosphorylation". Biochimica et ...
The vibrating ion-selective microelectrode was first used in 1990 to measure calcium fluxes in various cells and tissues. The ... Only the specific ion permeates the ionophore, therefore the voltage readout is proportional to the ion concentration in the ... Some ions (such as calcium) and molecules (such as hydrogen peroxide) modulate targeted translocators to produce a current or ... ionophore) is tip-filled into a previously silanized (to prevent leakage) microelectrode. Also, the microelectrode vibrates at ...
... such as calcium ionophore treatment, microinjection of calcium ions, or electrical stimulation. Treatment with cycloheximide, a ... The fertilization event causes intracellular calcium oscillations, and targeted degradation of cyclin B, a regulatory subunit ...
... and a lipid-soluble molecule that transports calcium ions (Ca2+) across the bilayer, also known as calcium ionophore. C1P was ... Inside the cell, S1P can induce calcium release independent of the S1PRs-the mechanism of which remains unknown. To date, the ... Tornquist, K. (February 2003). "Ceramide-1-phosphate increases intracellular free calcium concentrations in thyroid FRTL-5 ... and increase intracellular free calcium concentrations in thyroid cells. C1P also has known roles in vesicular trafficking, ...
Phoneutria nigriventer toxin-3
... and did not affect the release of glutamate evoked by a calcium ionophore (ionomycin). A polypeptide (Tx3-3) present in the ... This effect was most likely due to interference with the entry of calcium through voltage-gated calcium channels, because PhTx3 ... The PhTx3 neurotoxin is a broad-spectrum calcium channel blocker that inhibits glutamate release, calcium uptake and also ... We compared the alterations produced by the Tx3-3 with the actions of toxins known to block calcium channels coupled to ...
List of biomolecules
Calcium Ionophore) Abamectine Abietic acid Acetic acid Acetylcholine Actin Actinomycin D Adenine Adenosmeme Adenosine ...
... is a member of a family of naturally occurring cyclic ionophores known as the macrotetrolide antibiotics. The other ... Vishwanath, C. K.; Shamala, N.; Easwaran, K. R. K.; Vijayan, M. (1983). "Structure of nonactin-calcium perchlorate, C40H64O12. ... This ion selectivity is seen in other macrocyclic ligands, such as the cyclic ionophore valinomycin, which is also an ... Due to this property, nonactin is also called "ammonium ionophore". During complexation, the nonactin backbone convolutes into ...
... which releases calcium. This leads to an increase in mitochondrial calcium, dropping its membrane potential. The pore ... a polypeptide from the Red Sea Moses sole with ionophore activity". FEBS Letters. 242 (1): 161-6. doi:10.1016/0014-5793(88) ...
ISBN 978-3-527-30673-2. Sperelakis, Nicholas; Sperelakis, Nick (January 11, 2012). "Chapter 4: Ionophores in Planar Lipid ... mainly calcium, potassium, sodium, magnesium, iron, and manganese) by weight. Wood also contains sulfur, chlorine, silicon, ...
It also localizes to the acrosomal membrane of the sperm, where it putatively mediates calcium ion transmembrane transport. The ... "The use of anti-VDAC2 antibody for the combined assessment of human sperm acrosome integrity and ionophore A23187-induced ... In mammalian cardiomyocytes, VDAC2 promotes mitochondrial transport of calcium ions in order to power cardiac contractions. In ... Cell Calcium. 49 (2): 136-43. doi:10.1016/j.ceca.2010.12.004. PMID 21241999. Alvira CM, Umesh A, Husted C, Ying L, Hou Y, Lyu ...
Equine exertional rhabdomyolysis
... calcium ions are abnormally released. It is because of this that the calcium can become free-roaming which then can trigger ... Ingesting ionophores toxins, such as those found in day blooming jasmine, can cause the heart muscle to become inflamed, which ... When contracting muscles release intercellular calcium, however, if the horse has a predisposed condition that allows for ...
... a novel diacidic polyether antibiotic having high affinity for calcium ions". Journal of the American Chemical Society. 101 (12 ... unlike carrier ionophores. Examples of channel-forming ionophores are gramicidin A and nystatin. Ionophores that transport ... Ionophores can be selective to a particular ion but may not be exclusive to it. Ionophores facilitate the transport of ions ... However, these ionophores become unable to transport ions under very low temperatures. An example of a carrier ionophore is ...
... this calcium ion sensing ability is accommodated by an intracellular tail region on Slo-like subunits that form a calcium ion ... Ionophore and anthelmintic activity of PF 1022A, a cyclooctadepsipeptide, are not related - Gesner et al. Willson J; Amliwala K ... Role of calcium in neurotransmitter release evoked by alpha-latrotoxin or hypertonic sucrose - Khvotchev et al. A possible ... Schreiber M, Salkoff L (1997). "A novel calcium-sensing domain in the BK channel". Biophys. J. 73 (3): 1355-63. Bibcode:1997BpJ ...
The essential metals that lead interacts with include calcium, iron, and zinc. High levels of calcium and iron tend to provide ... A specific ion-selective electrode has been developed based on the ionophore S,S'-methylenebis (N,N-diisobutyldithiocarbamate ... Acute cases may require the use of disodium calcium edetate, the calcium chelate, and the disodium salt of ... By mimicking calcium, lead can cross the blood-brain barrier. It degrades the myelin sheaths of neurons, reduces their numbers ...
Transporter Classification Database
Family 1.A.106 The Calcium Load-activated Calcium Channel (CLAC) Family 1.A.107 The Pore-forming Globin (Globin) Family 1.B.1 ... Family 2.B.14 The Lasalocid A or X-537A Ionophore (Lasalocid) Family 2.B.15 The Tris-thiourea Tripodal-based Chloride Carrier ( ... Family 1.A.87 The Mechanosensitive Calcium Channel (MCA) Family 1.A.88 The Fungal Potassium Channel (F-Kch) Family 1.A.89 The ... Transportan Family 2.B.11 The Calcimycin or A23187 Carrier-type Ionophore Family 2.B.12 The Salinomycin Family 2.B.13 The ...
Similar solutions are formed by the heavy divalent alkaline earth metals calcium, strontium, barium, as well as the divalent ... carbohydrates and ionophore complexes. Lithium naturally only occurs in traces in biological systems and has no known ... Lithium and sodium are typically isolated through electrolysis from their liquid chlorides, with calcium chloride typically ... calcium (1.5%), and aluminium (1.4%); with the remaining 1.2% consisting of trace amounts of other elements. Due to planetary ...
When calcium binds to the troponin C, it causes conformational changes which lead to dislocation of troponin I. Afterwards, ... In veterinary medicine, increased cTnI has been noted from myocardial damage after ionophore toxicity in cattle. The high ... These changes alter the myofilament response to calcium, and are of interest in targeting heart failure. Multiple reaction ...
Calcium ionophore - XenWiki
Capacitation - Wikipedia
Calcium ionophore pre-treatment induces type-1 polarized DCs with enhanced T cell stimulatory function and IL-12 production |...
NHANES 2005-2006: Standard Biochemistry Profile Data Documentation, Codebook, and Frequencies
When the sample buffer mixture contacts the electrode, calcium ions complex with the ionophore at the electrode surface. ... LBXSCA - Total calcium (mg/dL). Variable Name: LBXSCA. SAS Label: Total calcium (mg/dL). English Text: Total calcium (mg/dL). ... LBDSCASI - Total calcium (mmol/L). Variable Name: LBDSCASI. SAS Label: Total calcium (mmol/L). English Text: Total calcium ( ... 7. Calcium The LX20 system uses indirect (or diluted) ISE methodology to measure calcium concentration in serum, plasma, or ...
Ana Maria Salicioni | Department of Veterinary and Animal Sciences
2016). Transient exposure to calcium ionophore enables in vitro fertilization in sterile mouse models. Scientific Reports, 6. ... 2015). Biphasic Role of Calcium in Mouse Sperm Capacitation Signaling Pathways. J Cell Physiol. presented at the 2015 Jan 17. ... 2000). S100P calcium-binding protein overexpression is associated with immortalization of human breast epithelial cells in ...
NIOSHTIC-2 Search Results - Basic View
Cell death and control of cell survival in the oligodendrocyte lineage: Cell
Becker's World of the Cell | Cell Biology | Biology | Science | Store | Pearson+
Acetyl-CoA: lyso-platelet-activating factor acetyltransferase activity in neutrophils from asthmatic and normal subjects -...
ATP amplifies NADPH-dependent and -independent neutrophil extracellular trap formation | Scientific Reports
... mice and that ATP contributes to NETosis induced in vitro by the calcium ionophore A23187 or phorbol 12-myristate 13-acetate ( ... NADPH-independent NETosis may not be enhanced by ARL67156 due to the already maximal effect caused by the calcium ionophore. In ... 3C,D), confirming that NETosis induced by the calcium ionophore was already maximal. Interestingly, we observed that adding ATP ... mice and that ATP contributes to NETosis induced in vitro by the calcium ionophore A23187 or phorbol 12-myristate 13-acetate ( ...
Frontiers | CamKII inhibitors reduce mitotic instability, connexon anomalies and progression of the in vivo behavioral...
Calcium ionophores and CamKII over-expression affect mitotic stability leading to centrosome over-duplication (Matsumoto and ... Matsumoto, Y., and Maller, J. L. (2002). Calcium, calmodulin, and CaMKII requirement for initiation of centrosome duplication ... Matsumoto and Maller (2002) demonstrated the relation between calcium flux, CamKII activity, and centrosome duplication. ... showing calcium-independent calmodulin-binding properties (Ahmad et al., 2001). Using a truncation mutant Cx32D215 they ...
A23187 from ALOMONE LABS
Titles list | Archive ouverte UNIGE
Calcium-induced insulin release in monolayer culture of the endocrine pancreas. Studies with ionophore A23187 Wollheim, Claes; ... Inhibition of insulin release by somatostatin: no evidence for interaction with calcium Wollheim, Claes; Blondel, B; Kikuchi, M ... Somatostatin inhibition of pancreatic glucagon release from monolayer cultures and interactions with calcium Wollheim, Claes; ...
IJMS | May 2018 - Browse Articles
Calcium ion levels in the capacitated sperm were reduced to a level similar to that in uncapacitated sperm after 90 min of ... Oviductal sperm was readily induced to undergo the acrosome reaction using the A23187 ionophore; however, the acrosome reaction ... Store-operated calcium entry (SOCE), a fundamentally important homeostatic and Ca2+ signaling pathway in many types of cells, ... Store-operated calcium entry (SOCE), a fundamentally important homeostatic and Ca2+ signaling pathway in many types of cells, ...
ERK1 activation is required for S-phase onset and cell cycle progression after fertilization in sea urchin embryos |...
Activation of sea-urchin eggs by a calcium ionophore. Proc. Natl. Acad. Sci. USA ... Ionophore activated eggs (white circle); eggs treated with U0126 15 minutes before ionophore activation (black circle). ... Ionophore activated eggs (white circle); eggs treated with U0126 15 minutes before ionophore activation (black circle). ... The relationship between calcium, MAP kinase, and DNA synthesis in Sea Urchin egg at fertilization. Dev. Biol. ...
Internet Scientific Publications
Evidence for mitochondrial control of neuronal polarity<...
Exposure of neurons to a calcium ionophore prevented axonogenesis. Confocal images of intracellular calcium levels and ... Exposure of neurons to a calcium ionophore prevented axonogenesis. Confocal images of intracellular calcium levels and ... Exposure of neurons to a calcium ionophore prevented axonogenesis. Confocal images of intracellular calcium levels and ... Exposure of neurons to a calcium ionophore prevented axonogenesis. Confocal images of intracellular calcium levels and ...
Volume 76 Issue 3 | Biology of Reproduction
Arf6-GEF BRAG1 Regulates JNK-Mediated Synaptic Removal of GluA1-Containing AMPA Receptors: A New Mechanism for Nonsyndromic X...
... examined the effects of calcium influx on mCherry-BRAG1 distribution in live HeLa cells stimulated with the calcium ionophore, ... The calcium-dependent release of CaM from BRAG1 suggests that changes in intracellular calcium levels may regulate the BRAG1- ... Thus, high calcium and magnesium bath solution (29 ± 1.5°C), containing the following (in mm): 119 NaCl, 2.5 KCl, 4 CaCl2, 4 ... C, Calcium-induced condensation of BRAG1 is reversible. HeLa cells were either left untreated (left), were treated with 5 μm ...
Molecular Vision: Perron, Mol Vis 2021; 27:151-160. Figure 1
Diverse stimuli engage different neutrophil extracellular trap pathways | eLife
A23187 is a calcium ionophore that causes a massive influx of calcium and nigericin stimulates potassium effluxes in cells ... the calcium ionophore A23187 which is produced during the growth of Streptomyces chartreusensis, (3) the potassium ionophore ... the calcium ionophore A23187, (3) the bacterial toxin nigericin that acts as a potassium ionophore, (4) the dimorphic fungus ... We examined the NETosis pathways induced by five stimuli; PMA, the calcium ionophore A23187, nigericin, Candida albicans and ...
Search Results | AVMA
Delayed Childbearing and Medically Assisted Reproduction
Phyllis Gardner's Profile | Stanford Profiles
In contrast, the calcium-activated chloride current was not affected by antisense treatment. Hence, the cAMP and calcium ... We show here that Ca2+ ionophore activates Cl- currents in cell-attached and whole-cell patch-clamp recordings of Jurkat T ... RECOMBINANT HUMAN TUMOR-NECROSIS-FACTOR-ALPHA INDUCES CALCIUM OSCILLATION AND CALCIUM-ACTIVATED CHLORIDE CURRENT IN HUMAN ... CALCIUM AND LYMPHOCYTE-T ACTIVATION CELL Gardner, P. 1989; 59 (1): 15-20 Abstract. A prolonged (at least 2-4 hr) elevation of [ ...
A23187IonomycinIntracellular calciumPhorbolVitroOocyte ActivationMonensinSelectiveStimulation with calciumInfluxCellsActivationPotassiumMembranesInducePathwayIonsPeritonealStallion spermatozoaQuantitationExtracellularExposureCalmodulinActivatorInhibitionMineralATPaseConcentrationChannelsConsiderablyMembraneProtein-tyrosiCompoundsInhibitorsSerumTreatmentNeuronalMetabolismChannel blockerProtocolsComplexes
- Calcium ionophore-stimulated chemiluminescence (CL) from human granulocytes: evidence that A23187-induced chemiluminescence originates from arachidonic acid metabolism. (cdc.gov)
- Here, we show that the ATP channel pannexin1 (Panx1) is functionally expressed by bone marrow-derived neutrophils (BMDNs) of wild-type (WT) mice and that ATP contributes to NETosis induced in vitro by the calcium ionophore A23187 or phorbol 12-myristate 13-acetate (PMA). (nature.com)
- A23187 is a mobile-carrier Ca2+ ionophore and can cause cell activation, differentiation, or proliferation and thus mimics cellular processes observed in response to cytokines and growth factors.Furthermore, A23187 induces in a concentration and time-dependent manner, Ca2+ triggered cell apoptosis. (linscottsdirectory.com)
- Cell death was induced by the calcium ionophore A23187, selecting a dose that reduces the MTT assay readout by 50% (1 μM). (molvis.org)
- The calcium ionophore, A23187, on the other hand, produced relaxation of the PV, which was significantly decreased by removing the endothelium. (aspetjournals.org)
- Then, the cells were treated for 2 hours to stimulate the protein kinase C (PKC) pathway via PGF2α and phorbol 12-myristat 13-acetate (PMA), and the calcium signaling pathway via the calcium ionophore (A23187). (unh.edu)
- Similarly, the calcium ionophore A23187 evoked dopamine release from control cells, but was ineffective in stimulating dopamine release from AS1 and AS2 cells. (elsevier.com)
- Effects of divalent cation ionophore A23187 on potassium permeability of rat erythrocytes. (semanticscholar.org)
- The induction of eicosanoid production by lipopolysaccharide and calcium ionophore A23187 in peritoneal macrophages and furosemide-induced PGE2 excretion was also abolished in cPLA2? (immune-source.com)
- It is possible that TR expression may be positively regulated by the calcium-signalling cascade, although TR induction by A23187 may be due to toxicity. (elsevier.com)
- Sampaio B, Ortiz I, Resende H, Felix M, Varner DD, Hinrichs K. Factors affecting intracellular calcium influx in response to calcium ionophore A23187 in equine sperm. (msdvetmanual.com)
- Variations in structural and functional changes of stallion spermatozoa in response to calcium ionophore A23187. (newhopefertility.com)
- A23187 is a calcium ionophore and a microbial metabolite that has been found in S. chartreusensis. (bertin-bioreagent.com)
- To stimulate the acrosome response capacitated sperm were treated with 3 μM calcium ionophore A23187 for 30 min. (conferencedequebec.org)
- Vascular efects induced by calcium ionophore A23187 in renal hypertensive rat ao. (usp.br)
- Endothelium-dependent relaxation was then assessed by cumulative addition of acetylcholine (ACh) or the calcium ionophore A23187. (uab.edu)
- was activated with a calcium mineral ionophore considerably, "type":"entrez-nucleotide","attrs":"text":"A23187″,"term_id":"833253″,"term_text":"A23187″A23187, but inhibited by phorbol-12-myristate-13-acetate, methylamine and monensin. (complextraitgenomics.com)
- MNS inhibits platelet aggregation caused by either the calcium ionophore A23187 (1 μM) or the protein kinase C (PKC) activator PDBu (200 nM) with IC50 of 25.9 μM and 4.8 μM, respectively. (invivochem.com)
- The ATP hydrolysis activity of the purified (Ca 2+ + Mg 2+ )-ATPase or the SR vesicles, in the presence of the Ca 2+ ionophore A23187, is not inhibited by T 3 or T 4 . (bgu.ac.il)
- The ATP hydrolysis activity of the purified (Ca2+ + Mg2+)-ATPase or the SR vesicles, in the presence of the Ca2+ ionophore A23187, is not inhibited by T3 or T4. (bgu.ac.il)
- The divalent cation ionophore A23187 was used in media containing 1.75-2.89 mM calcium to challenge explants with increased intracellular calcium . (musc.edu)
- For example, suboptimal concentrations of ionophores such as ionomycin exhibit slower Ca2+ efflux, which nevertheless ultimately produces the same net release as maximally effective concentrations (8). (edu.au)
- 2. We have studied potassium channels in human platelets by measuring the efflux of 86Rb+ (a marker for K+) from 86Rb(+)-loaded cells, and have defined their responses to stimulation by the platelet agonist thrombin and the calcium ionophore ionomycin. (ox.ac.uk)
- Some assisted activation treatments such as Ca2+ (calcium)-ionophore, strontium chloride (vzorec) and ionomycin, promote an increase in intracellular free calcium concentrations by the release of calcium from cytoplasmic stores. (fertilitypedia.org)
- Treatment with the calcium ionophore ionomycin induced cell death and neurite degeneration in a concentration- and time-dependent manner. (elsevier.com)
- Treatment with a low concentration of ionomycin immediately produced a significant calcium influx into the intracellular region in N1E-115 cells. (elsevier.com)
- These results indicate that ionomycin induces calcium influx into the intracellular region and reactive oxygen species production in N1E-115 cells. (elsevier.com)
- A similar alkalinization response was produced by the calcium ionophore, ionomycin and by the calcium ATPase inhibitor, thapsigargin. (umn.edu)
- Finally, the ionophore ionomycin and the Ca2+ ATPase blocker thapsigargin induce the same age related effect on beta-endorphin release. (omeka.net)
- Synaptic vesicles isolated from sheep brain cortex exhibit an ATP-dependent Ca2+ accumulation that is inhibited by the protonophore uncoupler carbonyl cyanide m-chorophenylhydrazone (CCCP) and completely released by the Ca2+ ionophore ionomycin. (uc.pt)
- Ionomycin is an ionophore, a compound that binds calcium ions reversibly, and is used to transport calcium ions across the cell's plasma membrane, increasing cellular calcium concentration. (smpdb.ca)
- Phorbal esters, in conjunction with ionomycin, are used in this case to study the cellular response to DAG and calcium signalling without needing the cells to undergo other stimulation, mimicking things like T-cell activation easily. (smpdb.ca)
- Basal levels of intracellular calcium were elevated 2- to 3-fold, intramitochondrial calcium levels were greatly increased, and mitochondrial transmembrane potential was decreased in EtBr-treated neurons. (elsevier.com)
- Confocal images of intracellular calcium levels and mitochondrial localization in the same cells revealed congregations of mitochondria at the base of the axon associated with local reductions of intracellular calcium levels. (elsevier.com)
- Intracellular calcium was monitored by fluorescence spectrophotometry. (elifesciences.org)
- This loss of physical interaction with HSPCs could be partially restored by treating the MSCs with calcium ionophore or calmodulin, suggesting that NF-κB regulates intracellular calcium flux in the MSCs. (therapeuticsolutionsint.com)
- IP3 then activates an IP3 sensitive calcium channel, increasing the concentration of intracellular calcium. (smpdb.ca)
- These findings demonstrate that increased intracellular calcium produces necrotic changes in spinal cord tissue much more rapidly than increased extracellular calcium . (musc.edu)
- Following stimulation of murine splenocytes with phorbol ester and calcium ionophore, we observed differences in expression of CD69, major histocompatibility complex class II molecules, the glucocorticoid-induced TNF receptor family-related gene product, and surface immunoglobulin M and D that were subsequently confirmed by Western blot or flow cytometric analysis. (ox.ac.uk)
- A phorbol ester and calcium ionophore regulate sex steroid and prostaglandin release by follicles of the anuran Rana esculenta and the urodele Triturus carnifex. (unicam.it)
- The degranulation of mast cells may also be induced with the artificial A-770041 substance 48/80, phorbol 12-myristate 13-acetate (PMA), and calcium mineral ionophore. (cancer-basics.com)
- Andrews DA, Yang L, Low PS: Phorbol ester stimulates a protein kinase C-mediated agatoxin-TK-sensitive calcium permeability pathway in human red blood cells. (smpdb.ca)
- In vitro binding studies have demonstrated that zonisamide binds to the GABA/benzodiazepine receptor ionophore complex in an allosteric fashion which does not produce changes in chloride flux. (nih.gov)
- In vitro analyses of the Y152C mutation indicated a pathology similar to other known mutations in KCNJ5, including change in conductance to Na+ ions and elevated calcium levels, and increase in CYP11B2 mRNA and aldosterone production. (openrepository.com)
- To further evaluate the direct effects of calcium on spinal cord tissue , without consideration of the vasculature an in vitro system was employed . (musc.edu)
- These calcium-induced changes were qualitatively similar to those observed following trauma in vitro . (musc.edu)
- These findings indicate that calcium-induced toxicity in vitro can be prevented to a significant degree with possible therapeutic applications following spinal cord trauma in vivo . (musc.edu)
- Interestingly, transient treatments with either Ca 2+ ionophore, starvation, or a combination of both restore the fertilization capacity of C2CD6-deficient sperm in vitro. (biorxiv.org)
- Apart from structural changes and meiosis resumption, there are basically two main events that characterize oocyte activation: the calcium release and the electrical events. (fertilitypedia.org)
- The elevation of free intracellular (inside the cell) calcium is a nearly universal signal that triggers the cascade of events that leads to oocyte activation. (fertilitypedia.org)
- The aim of artificial oocyte activation is to mimic physiological mechanisms, based mainly on calcium changes. (fertilitypedia.org)
- The massive increase in cytosolic calcium occurs in periodic oscillations with amplitude and frequency that are crucial for the success of oocyte activation and embryo development. (fertilitypedia.org)
- Calcium oscillations cease when pronuclei are formed, which is considered to be the end point of oocyte activation. (fertilitypedia.org)
- 21) Meiri H, Erulkar SD, Lerman T, Rahamimoff R. The action of the sodium ionophore, monensin, on transmitter release at the frog neuromuscular junction. (libguides.com)
- Ionophores, such as monensin, are included in feed mainly to increase weight gain and to prevent bovine coccidiosis ( 3 , 4 ). (cdc.gov)
- Identification of a selective manganese ionophore that enables nonlethal quantification of cellular manganese. (vanderbilt.edu)
- The design of Selective ion carriers have been of considerable ionophores for Li+ is a di cult problem owing to interest as tools for the analysis and separation of the fact that lithium ions are smaller than both metal ions as well as for many biological applica- sodium and potassium ions but are strongly tions. (healthdrugpdf.com)
- We have previously reported the synthesis and X-ray struc- Acyclic Li+ ionophores 1±3, which are good tural characterization of ACIA 9 carrying a neutral carriers in Li+-selective electrodes, may monoazacrown moiety . (healthdrugpdf.com)
- C2CD6 interacts with the CatSper calcium-selective core forming subunits. (biorxiv.org)
Stimulation with calcium1
- Deficient granulocytes had significantly increased numbers of Mo1 antigen expressed on the surface following stimulation with calcium ionophore (1 microM). (amsterdamumc.org)
- Calcium chloride is added to facilitated the influx via of calcium cations. (wikipedia.org)
- Others such as electrical stimulus promote influx of calcium from the extracellular medium and some treatments such as ethanol promote both effects. (fertilitypedia.org)
- Calcium influx into the intracellular region is a possible activator of neurite degeneration. (elsevier.com)
- Calcium influx caused by the CatSper activation results in powerful asymmetrical flagellar beating movement known as hyperactivation. (biorxiv.org)
- Similar to results observed with OVCAR-8 cells, calcium treatment and STAT3 knock-down in two other ovarian cancer cell lines, OVCAR-3 and SK-OV-3 cells, resulted in increased CCN1 expression. (unh.edu)
- In the presence of extracellular calcium, a depolarizing concentration of K + (56 mM) evoked dopamine release from control cells, but not from AS1 and AS2 cells. (elsevier.com)
- Because the expression of antisense GAP‐43 RNA in PC12 cells leads to a decrease in GAP‐43 expression and to the loss of evoked dopamine release, these results provide evidence of a role for GAP‐43 in calcium‐dependent neurotransmitter release. (elsevier.com)
- Transport via the regulated secretory pathway in semi-intact PC12 cells: role of intra-cisternal calcium and pH in the transport and sorting of secretogranin II. (rupress.org)
- 20152,35484} It induces calcium overload and mitochondrial apoptosis in HEK293 cells when used at a concentration of 5 µM. (bertin-bioreagent.com)
- Pilot data including treatment of primary cells with calcium ionophores indicated that ZF/ZR cells may have a phenotype that is 'muted' for calcium mediated pathways. (openrepository.com)
- In tumour cells, U90 is located principally in the plasma membrane fraction and cannot be induced by heat shock, glucose starvation, or treatment with tunicamycin or calcium ionophore. (microbiologyresearch.org)
- Mast cells are turned on by the procedure of degranulation, which sets off the discharge of mediators such as for example histamine by calcium mineral signaling. (cancer-basics.com)
- The effects of a conjugated linoleic acid (CLA) mixture of single isomers (50:50, w/w, cis9,trans11:trans10,cis12) and the individual isomers on (a) the production of resting and calcium ionophore stimulated 14C-eicosanoids and (b) the incorporation of 14C-arachidonic acid (AA) into membrane phospholipids of human saphenous vein endothelial cells were investigated. (brad.ac.uk)
- Shikonin is shown to shuttle glucose into muscle cells by directly increasing the calcium concentration in skeletal muscle tissue , mimicking muscle contraction. (priceplow.com)
- For these reasons, we suggest that calcium homeostasis might be important for the differences observed in the release of the opioid from cells obtained from younger ( or = 45 years) volunteers. (omeka.net)
- These findings indicate calcium ions induce increased vesicular transport by the endothelial cells of the blood-spinal cord barrier indicating a pathway by which calcium ions alter the extracellular milieu of axons and glial cells . (musc.edu)
- We further discovered that the ionophore treatment improved the active type of the tiny GTPase Rac1 in these cells. (cisplatin.info)
- b) Snapshots from time-lapse calcium mineral imaging of one cells. (cisplatin.info)
- Activation of MAP kinases by calcium-dependent and calcium-independent pathways. (elsevier.com)
- Their activation leads to calcium release. (fertilitypedia.org)
- The activation of MAP kinase in human being neutrophils stimulated by both uncoated and plasma-opsonized crystals of triclinic calcium pyrophosphate dihydrate (CPPD) was investigated. (mg-132.net)
- Oxcarbazepine's anticonvulsant effect may occur by affecting potassium conductance and high-voltage activated calcium channels. (medscape.com)
- Phosphatidylserine exposure and calcium-activated potassium efflux in platelets. (harvard.edu)
- Pharmacological evidence of calcium-activated and voltage-gated potassium channels in human platelets. (ox.ac.uk)
- Vesicles were loaded with 45 Ca 2+ and membrane potentials were generated by establishing potassium gradients across the vesicle using the ionophore valinomycin. (elsevier.com)
- The presence of calcium ion increased the ¯uorescence slightly, but potassium and sodium had no e ect. (healthdrugpdf.com)
- Interaction of charged and uncharged calcium channel antagonists with phospholipid membranes : binding equilibrium, binding enthalpy, and membrane location. (unibas.ch)
- Oz, M, Tchugunova, Y & Dinc, M 2004, ' Differential effects of endogenous and synthetic cannabinoids on voltage-dependent calcium fluxes in rabbit T-tubule membranes: Comparison with fatty acids ', European Journal of Pharmacology , vol. 502, no. 1-2, pp. 47-58. (elsevier.com)
- The treatment of BAECs with 30 bcg/mL cholesterol for 24 hours resulted in significant rises in total eNOS protein expression, eNOS associated with caveolae-enriched membranes, and calcium ionophore-stimulated NO production boosted by calcium ionophore-stimulated NO production. (plex.page)
- The multi-subunit CatSper complexes form highly organized calcium signaling nanodomains on flagellar membranes. (biorxiv.org)
- Calcium ionophores are also ideal to induce capacitation. (wikipedia.org)
- We show that PMA, C. albicans and GBS use a related pathway for NET induction, whereas ionophores require an alternative pathway but that NETs produced by all stimuli are proteolytically active, kill bacteria and composed mainly of chromosomal DNA. (elifesciences.org)
- In this pathway, they are shown to activate protein kinase C in the same way as calcium does, as well as mimicking the action of DG. (smpdb.ca)
- 28) Rahamimoff R, Erulkar SD, Alnaes E, Meiri H, Rotshenker S, Rahamimoff H. Modulation of transmitter release by calcium ions and nerve impulses. (libguides.com)
- Crude chloroform extracts of fresh feverfew leaves (rich in sesquiterpene lactones) and of commercially available powdered leaves (lactone-free) produced dose-dependent inhibition of the generation of thromboxane B2 (TXB2) and leukotriene B4 (LTB4) by ionophore- and chemoattractant-stimulated rat peritoneal leukocytes and human polymorphonuclear leukocytes. (henriettesherbal.com)
- Testisin localization, before and after exposure of stallion spermatozoa to capacitating conditions and calcium ionophore, was examined using live cell immunofluorescence and flow cytometry. (edu.au)
- Detection and quantitation of a variety of ionophore compounds by liquid chromatography / mass spectrometry. (tamu.edu)
- Quantitation of surface Mo1 on granulocytes exposed to calcium ionophore (1 microM) showed that both parents in one family but only the mother in the other family had significantly reduced levels of Mo1, suggesting heterogeneity in the inheritance of this disorder. (amsterdamumc.org)
- Experimental explants were challenged with ten times the normal concentrations of extracellular calcium (17.5-29.0 mM) . (musc.edu)
- Exposure of neurons to a calcium ionophore prevented axonogenesis. (elsevier.com)
- Double exposure to calcium ionophore may improve blastocyst development and clinical pregnancy in some patients. (planer.com)
- The calcium ionophore A23157 increased TR expression in HUVECs after a 12-h exposure, but the maximal effect was only observed after a 35-h exposure. (elsevier.com)
- Zinc, selenium, vitamin D, and Zinc Ionophore supplementation via dietary supplements and/or food sources (oyster, liver…), sunshine (UVB) exposure are more important than ever to have dialed-in. (advancedhealing.com)
- Here, we show that the BRAG1 IQ motif binds apo-calmodulin (CaM), and that calcium-induced CaM release triggers a reversible conformational change in human BRAG1. (jneurosci.org)
- The specific objectives were (1) to determine the regulation of CCN1 expression by prostaglandin-F2α (PGF2α), signal transducer and activator of transcription 3 (STAT3) and calcium, and (2) to identify the respective cellular signaling molecules that played a role in regulating CCN1 expression. (unh.edu)
- Disruption of organelle pH gradients with an ionophore or a weak base resulted in the inhibition of transport via both the constitutive and the regulated pathways. (rupress.org)
- Ionophores, known by the brand names Rumensin and Bovatec, increase cell acidity and growth inhibition. (americandairymen.com)
- In contrast, the activity of the H+-ATPase, measured under uncoupling conditions (presence of CCCP), appears to be unaltered by the calcium ion. (uc.pt)
- Leukotriene B 4 (LTB 4 ) concentration was determined in gastric mucosa and in whole blood after ex vivo stimulation with a calcium ionophore. (avma.org)
- Zonisamide may produce these effects through action at sodium and calcium channels. (nih.gov)
- The first calcium rise may be followed by an entry of calcium across the plasma membrane through voltage-sensitive calcium channels that respond to membrane depolarization. (fertilitypedia.org)
- These results indicate that endocannabinoids, but not synthetic cannabinoids, directly inhibit the function of voltage-dependent calcium channels (VDCCs) and modulate the specific binding of calcium channel ligands of the dihydropyridine (DHP) class. (elsevier.com)
- We also demonstrated that inhibiting the intracellular Ca2+ retarded the gastrulation cell motions considerably, while raising the intracellular Ca2+ with an ionophore improved the migration. (cisplatin.info)
- The calcium can activate protein-tyrosine kinase 2-beta, and can also activate protein kinase C. DG also works to activate protein kinase C in the cell. (smpdb.ca)
- These compounds as well as PAF produce significant increases in cytoplasmic calcium ([Ca 2+ ] i ). (umn.edu)
- We studied NET production in neutrophils from healthy donors with inhibitors of molecules crucial to PMA-induced NETs including protein kinase C, calcium, reactive oxygen species, the enzymes myeloperoxidase (MPO) and neutrophil elastase. (elifesciences.org)
- Elevated total serum calcium levels are associated with idiopathic hypercalcemia, vitamin D intoxication, hyperparathyroidism, sarcoidosis, pneumocystic carinii pneumonia, and blue diaper syndrome. (cdc.gov)
- Calcium ionophore treatment is now the only commonly used method of AOA. (fertilitypedia.org)
- When taken together with previous data indicating important roles for calcium in regulating neurite outgrowth, the present findings suggest critical roles for mitochondrial function and modulation of calcium homeostasis in the establishment of neuronal polarity. (elsevier.com)
- Functional cell-based assays often monitor for changes in neuronal activity with calcium imaging, patch clamp electrophysiology, or label-free microelectrode array (MEA) recordings . (axionbiosystems.com)
- We also demonstrate calcium channel blocker, verapamil as an efficient blocker of mKCNJ5 mediated aldosterone production. (openrepository.com)
- In D. G. Lambert (Ed.), Calcium Signaling Protocols (Vol. 114, pp. 235-248). (edu.au)
- We have previously shown that volatile anesthetics inhibit glutamate-stimulated [ 3 H]MK-801 binding to the ionophore of NMDA receptor complexes in rat brain. (elsevier.com)