Chemical agents that increase the permeability of biological or artificial lipid membranes to specific ions. Most ionophores are relatively small organic molecules that act as mobile carriers within membranes or coalesce to form ion permeable channels across membranes. Many are antibiotics, and many act as uncoupling agents by short-circuiting the proton gradient across mitochondrial membranes.
Cationic ionophore antibiotic obtained from Streptomyces lasaliensis that, among other effects, dissociates the calcium fluxes in muscle fibers. It is used as a coccidiostat, especially in poultry.
An ionophorous, polyether antibiotic from Streptomyces chartreusensis. It binds and transports CALCIUM and other divalent cations across membranes and uncouples oxidative phosphorylation while inhibiting ATPase of rat liver mitochondria. The substance is used mostly as a biochemical tool to study the role of divalent cations in various biological systems.
A divalent calcium ionophore that is widely used as a tool to investigate the role of intracellular calcium in cellular processes.
Chemical agents that increase the permeability of CELL MEMBRANES to CALCIUM ions.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
A chelating agent relatively more specific for calcium and less toxic than EDETIC ACID.
A phorbol ester found in CROTON OIL with very effective tumor promoting activity. It stimulates the synthesis of both DNA and RNA.
Signal transduction mechanisms whereby calcium mobilization (from outside the cell or from intracellular storage pools) to the cytoplasm is triggered by external stimuli. Calcium signals are often seen to propagate as waves, oscillations, spikes, sparks, or puffs. The calcium acts as an intracellular messenger by activating calcium-responsive proteins.
A polyether antibiotic which affects ion transport and ATPase activity in mitochondria. It is produced by Streptomyces hygroscopicus. (From Merck Index, 11th ed)
An antiprotozoal agent produced by Streptomyces cinnamonensis. It exerts its effect during the development of first-generation trophozoites into first-generation schizonts within the intestinal epithelial cells. It does not interfere with hosts' development of acquired immunity to the majority of coccidial species. Monensin is a sodium and proton selective ionophore and is widely used as such in biochemical studies.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
A cyclododecadepsipeptide ionophore antibiotic produced by Streptomyces fulvissimus and related to the enniatins. It is composed of 3 moles each of L-valine, D-alpha-hydroxyisovaleric acid, D-valine, and L-lactic acid linked alternately to form a 36-membered ring. (From Merck Index, 11th ed) Valinomycin is a potassium selective ionophore and is commonly used as a tool in biochemical studies.
Voltage-dependent cell membrane glycoproteins selectively permeable to calcium ions. They are categorized as L-, T-, N-, P-, Q-, and R-types based on the activation and inactivation kinetics, ion specificity, and sensitivity to drugs and toxins. The L- and T-types are present throughout the cardiovascular and central nervous systems and the N-, P-, Q-, & R-types are located in neuronal tissue.
Calcium compounds used as food supplements or in food to supply the body with calcium. Dietary calcium is needed during growth for bone development and for maintenance of skeletal integrity later in life to prevent osteoporosis.
Chemical agents that increase the permeability of CELL MEMBRANES to POTASSIUM ions.
Carbonic acid calcium salt (CaCO3). An odorless, tasteless powder or crystal that occurs in nature. It is used therapeutically as a phosphate buffer in hemodialysis patients and as a calcium supplement.
A proton ionophore. It is commonly used as an uncoupling agent and inhibitor of photosynthesis because of its effects on mitochondrial and chloroplast membranes.
A proton ionophore that is commonly used as an uncoupling agent in biochemical studies.
Chemical agents that increase the permeability of CELL MEMBRANES to SODIUM ions.
Calcium salts of phosphoric acid. These compounds are frequently used as calcium supplements.
Stable calcium atoms that have the same atomic number as the element calcium, but differ in atomic weight. Ca-42-44, 46, and 48 are stable calcium isotopes.
A salt used to replenish calcium levels, as an acid-producing diuretic, and as an antidote for magnesium poisoning.
A group of peptide antibiotics from BACILLUS brevis. Gramicidin C or S is a cyclic, ten-amino acid polypeptide and gramicidins A, B, D are linear. Gramicidin is one of the two principal components of TYROTHRICIN.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
The rate dynamics in chemical or physical systems.
An element in the alkali group of metals with an atomic symbol K, atomic number 19, and atomic weight 39.10. It is the chief cation in the intracellular fluid of muscle and other cells. Potassium ion is a strong electrolyte that plays a significant role in the regulation of fluid volume and maintenance of the WATER-ELECTROLYTE BALANCE.
The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization).
Long-lasting voltage-gated CALCIUM CHANNELS found in both excitable and nonexcitable tissue. They are responsible for normal myocardial and vascular smooth muscle contractility. Five subunits (alpha-1, alpha-2, beta, gamma, and delta) make up the L-type channel. The alpha-1 subunit is the binding site for calcium-based antagonists. Dihydropyridine-based calcium antagonists are used as markers for these binding sites.
The movement of materials across cell membranes and epithelial layers against an electrochemical gradient, requiring the expenditure of metabolic energy.
Compounds with a 5-membered ring of four carbons and an oxygen. They are aromatic heterocycles. The reduced form is tetrahydrofuran.
The calcium salt of oxalic acid, occurring in the urine as crystals and in certain calculi.
The calcium salt of gluconic acid. The compound has a variety of uses, including its use as a calcium replenisher in hypocalcemic states.
A member of the alkali group of metals. It has the atomic symbol Na, atomic number 11, and atomic weight 23.
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
Unstable isotopes of calcium that decay or disintegrate emitting radiation. Ca atoms with atomic weights 39, 41, 45, 47, 49, and 50 are radioactive calcium isotopes.
An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.
Lanthanum. The prototypical element in the rare earth family of metals. It has the atomic symbol La, atomic number 57, and atomic weight 138.91. Lanthanide ion is used in experimental biology as a calcium antagonist; lanthanum oxide improves the optical properties of glass.
A metallic element that has the atomic symbol Mg, atomic number 12, and atomic weight 24.31. It is important for the activity of many enzymes, especially those involved in OXIDATIVE PHOSPHORYLATION.
Electrodes which can be used to measure the concentration of particular ions in cells, tissues, or solutions.
Metallochrome indicator that changes color when complexed to the calcium ion under physiological conditions. It is used to measure local calcium ion concentrations in vivo.
Chemical agents that increase the permeability of CELL MEMBRANES to PROTONS.
Solution titration in which the end point is read from the electrode-potential variations with the concentrations of potential determining ions. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A sesquiterpene lactone found in roots of THAPSIA. It inhibits CA(2+)-TRANSPORTING ATPASE mediated uptake of CALCIUM into SARCOPLASMIC RETICULUM.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
A calcium channel blocker that is a class IV anti-arrhythmia agent.
Inorganic compounds that contain calcium as an integral part of the molecule.
Chemicals that bind to and remove ions from solutions. Many chelating agents function through the formation of COORDINATION COMPLEXES with METALS.
CALCIUM CHANNELS that are concentrated in neural tissue. Omega toxins inhibit the actions of these channels by altering their voltage dependence.
A suborder of the order ARTIODACTYLA whose members have the distinguishing feature of a four-chambered stomach, including the capacious RUMEN. Horns or antlers are usually present, at least in males.
A toxic dye, chemically related to trinitrophenol (picric acid), used in biochemical studies of oxidative processes where it uncouples oxidative phosphorylation. It is also used as a metabolic stimulant. (Stedman, 26th ed)
Drugs used by veterinarians in the treatment of animal diseases. The veterinarian's pharmacological armamentarium is the counterpart of drugs treating human diseases, with dosage and administration adjusted to the size, weight, disease, and idiosyncrasies of the species. In the United States most drugs are subject to federal regulations with special reference to the safety of drugs and residues in edible animal products.
A carbodiimide that is used as a chemical intermediate and coupling agent in peptide synthesis. (From Hawley's Condensed Chemical Dictionary, 12th ed)
Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.
Stable elementary particles having the smallest known positive charge, found in the nuclei of all elements. The proton mass is less than that of a neutron. A proton is the nucleus of the light hydrogen atom, i.e., the hydrogen ion.
2-Hydroxy-N-phenylbenzamides. N-phenyl substituted salicylamides. Derivatives have been used as fungicides, anti-mildew agents and topical antifungal agents. In concentrated form may cause irritation of skin and mucous membranes.
Agents that increase calcium influx into calcium channels of excitable tissues. This causes vasoconstriction in VASCULAR SMOOTH MUSCLE and/or CARDIAC MUSCLE cells as well as stimulation of insulin release from pancreatic islets. Therefore, tissue-selective calcium agonists have the potential to combat cardiac failure and endocrinological disorders. They have been used primarily in experimental studies in cell and tissue culture.
Agents useful in the treatment or prevention of COCCIDIOSIS in man or animals.
Substances that reduce the growth or reproduction of BACTERIA.
Elements of limited time intervals, contributing to particular results or situations.
Positively charged atoms, radicals or groups of atoms with a valence of plus 2, which travel to the cathode or negative pole during electrolysis.
Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.
A class of compounds composed of repeating 5-carbon units of HEMITERPENES.
An acidifying agent that has expectorant and diuretic effects. Also used in etching and batteries and as a flux in electroplating.
Quinolines substituted in any position by one or more amino groups.
Established cell cultures that have the potential to propagate indefinitely.
A quality of cell membranes which permits the passage of solvents and solutes into and out of cells.
The relationship between the dose of an administered drug and the response of the organism to the drug.
Chemical agents that uncouple oxidation from phosphorylation in the metabolic cycle so that ATP synthesis does not occur. Included here are those IONOPHORES that disrupt electron transfer by short-circuiting the proton gradient across mitochondrial membranes.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
A highly poisonous compound that is an inhibitor of many metabolic processes, but has been shown to be an especially potent inhibitor of heme enzymes and hemeproteins. It is used in many industrial processes.
A chelating agent that sequesters a variety of polyvalent cations such as CALCIUM. It is used in pharmaceutical manufacturing and as a food additive.
Positively charged atoms, radicals or groups of atoms which travel to the cathode or negative pole during electrolysis.
A white powder prepared from lime that has many medical and industrial uses. It is in many dental formulations, especially for root canal filling.
Artificial, single or multilaminar vesicles (made from lecithins or other lipids) that are used for the delivery of a variety of biological molecules or molecular complexes to cells, for example, drug delivery and gene transfer. They are also used to study membranes and membrane proteins.
A non-metal element that has the atomic symbol P, atomic number 15, and atomic weight 31. It is an essential element that takes part in a broad variety of biochemical reactions.
The first stomach of ruminants. It lies on the left side of the body, occupying the whole of the left side of the abdomen and even stretching across the median plane of the body to the right side. It is capacious, divided into an upper and a lower sac, each of which has a blind sac at its posterior extremity. The rumen is lined by mucous membrane containing no digestive glands, but mucus-secreting glands are present in large numbers. Coarse, partially chewed food is stored and churned in the rumen until the animal finds circumstances convenient for rumination. When this occurs, little balls of food are regurgitated through the esophagus into the mouth, and are subjected to a second more thorough mastication, swallowed, and passed on into other parts of the compound stomach. (From Black's Veterinary Dictionary, 17th ed)
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
A potent vasodilator agent with calcium antagonistic action. It is a useful anti-anginal agent that also lowers blood pressure.
A potentially neurotoxic 8-hydroxyquinoline derivative long used as a topical anti-infective, intestinal antiamebic, and vaginal trichomonacide. The oral preparation has been shown to cause subacute myelo-optic neuropathy and has been banned worldwide.
A calcium salt that is used for a variety of purposes including: building materials, as a desiccant, in dentistry as an impression material, cast, or die, and in medicine for immobilizing casts and as a tablet excipient. It exists in various forms and states of hydration. Plaster of Paris is a mixture of powdered and heat-treated gypsum.
The concentration of osmotically active particles in solution expressed in terms of osmoles of solute per liter of solution. Osmolality is expressed in terms of osmoles of solute per kilogram of solvent.
Organic compounds that contain two nitro groups attached to a phenol.
A polypeptide hormone (84 amino acid residues) secreted by the PARATHYROID GLANDS which performs the essential role of maintaining intracellular CALCIUM levels in the body. Parathyroid hormone increases intracellular calcium by promoting the release of CALCIUM from BONE, increases the intestinal absorption of calcium, increases the renal tubular reabsorption of calcium, and increases the renal excretion of phosphates.
An atom or group of atoms that have a positive or negative electric charge due to a gain (negative charge) or loss (positive charge) of one or more electrons. Atoms with a positive charge are known as CATIONS; those with a negative charge are ANIONS.
Artificially produced membranes, such as semipermeable membranes used in artificial kidney dialysis (RENAL DIALYSIS), monomolecular and bimolecular membranes used as models to simulate biological CELL MEMBRANES. These membranes are also used in the process of GUIDED TISSUE REGENERATION.
An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.
Organic compounds containing the carboxy group (-COOH). This group of compounds includes amino acids and fatty acids. Carboxylic acids can be saturated, unsaturated, or aromatic.
Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.
A fluorescent calcium chelating agent which is used to study intracellular calcium in tissues.

Changes in content and localization of proteins phosphorylated at tyrosine, serine and threonine residues during ram sperm capacitation and acrosome reaction. (1/68)

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The use of anti-VDAC2 antibody for the combined assessment of human sperm acrosome integrity and ionophore A23187-induced acrosome reaction. (2/68)

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Ultrastructure and intracellular calcium response during activation in vitrified and slow-frozen human oocytes. (3/68)

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Effects of FK506 and cyclosporin a on calcium ionophore-induced mitochondrial depolarization and cytosolic calcium in astrocytes and neurons. (4/68)

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Calpain-cleaved type 1 inositol 1,4,5-trisphosphate receptor (InsP(3)R1) has InsP(3)-independent gating and disrupts intracellular Ca(2+) homeostasis. (5/68)

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Activation of human vascular cells decreases their expression of transforming growth factor-beta. (6/68)

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Chromatin disruption in the promoter of bovine leukemia virus during transcriptional activation. (7/68)

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The redox state of transglutaminase 2 controls arterial remodeling. (8/68)

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Sophoricoside exhibits numerous pharmacological effects, including anti- inflammatory and anti-cancer actions, yet the exact mechanism that accounts for the anti-allergic effects of sophoricoside is not completely understood. The aim of the present study was to elucidate whether and how sophoricoside modulates the mast cell-mediated allergic inflammation in vitro and in vivo. We investigated the pharmacological effects of sophoricoside on both compound 48/80 or histamine-induced scratching behaviors and 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis in mice. Additionally, to find a possible explanation for the anti-inflammatory effects of sophoricoside, we evaluated the effects of sophoricoside on the production of histamine and inflammatory cytokines and activation of nuclear factor-κB (NF-κB) and caspase-1 in phorbol 12-myristate 13-acetate plus calcium ionophore A23187 (PMACI)-stimulated human mast cells (HMC-1). The finding of this study demonstrated that sophoricoside reduced
The data presented above indicate that Ano6, 8 and 9, when overexpressed in HEK293 cells, produce Cl− currents upon increase in intracellular Ca2+ by ionomycin. However, although all three paralogs are expressed endogenously in HEK293 cells, no currents are activated in mock-transfected cells (Fig. 3A) (Almaça et al., 2009). However, when increasing [Ca2+]i to very high levels (≥10 µM), endogenous anoctamins were activated (Fig. 3B,E). This may be due to a lack of sufficient membrane expression of endogenous anoctamins. Endogenous anoctamins are expressed at much lower levels than overexpressed proteins and may be inhibited by endogenous accessory proteins, such as CAMKII. Alternatively, specific Ca2+ release and/or influx pathways need to be triggered to activate these endogenous anoctamins. The overall linear structures appear similar in all anoctamins (supplementary material Fig. S1) and an analysis of putative functional domains revealed a number of similar motifs and putative ...
Volumenregulation ist ein grundlegender Mechanismus, der für alle lebenden Organismen notwendig ist. Zellen schwellen unter einem hypo- osmotischen Schock und aktivieren darauf die Freisetzung von K+ und Cl- Ionen über die Aktivierung von K+- und Cl-- Kanälen, um das Ausgangsvolumen wieder herzustellen. Im Jahre 2014 wurde von zwei unabhängigen Arbeitsgruppen LRRC8A als Hauptkomponente des Volumen- Regulierten Anionen Canals (VRAC) identifiziert. Die Ergebnisse der vorliegenden Arbeit lassen aber vermuten, dass auch Anoctamine und Bestrophine an der Volumenregulation beteiligt sind. Darüber hinaus konnte für diese Proteine der Mechanismus ihrer zellvolumen-abhängigen Aktivierung geklärt werden. Zusätzlich wurde in dieser Arbeit die Rolle der TMC Proteine (TransMembrane Channel-like family of Proteins), die eine hohe Sequenzähnlichkeit zu den Anoctaminen besitzen, für die Volumenregulation und für die Regulation der intrazellulären Ca2+ Konzentration untersucht. Überexpression von ...
Histopathological assessment of the anti-osteoporosis effect of sophoricoside.Normal group (A) shows normal bony tissue with intact well-formed dense trabeculae
Shop Phospholipid scramblase ELISA Kit, Recombinant Protein and Phospholipid scramblase Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Anoctamins and Bestrophins are two kinds of calcium-activated chloride channels. Anoctamins were shown to be Ca-activated Cl channels in 2008 and are responsible for the classical CaCCs that are widely expressed in many tissues, especially epithelia. Bestrophins are more restricted in their expression and play more specialized roles. Bestrophin-1 is expressed in the retinal pigment epithelium and certain mutations in bestrophin-1 cause degeneration of the retina and blindness. Bestrophin-2 is expressed in goblet cells in colon and may play a role in bicarbonate secretion in this tissue. We are interested in understanding how both anoctamins and bestrophins are regulated by calcium. We are identifying the calcium binding sites and the molecular links between the calcium sensor and the channel gate using a combination of both whole-cell and single channel electrophysiological mesurements and mutagenesis. We are dissecting the signaling protein network associated with these channels using ...
Antibodies for proteins involved in phospholipid scramblase activity pathways, according to their Panther/Gene Ontology Classification
Buy PLSCR4 elisa kit, Human Phospholipid scramblase 4 ELISA Kit-Q9NRQ2.2 (MBS283171) product datasheet at MyBioSource, ELISA Kits
Intercellular adhesion molecule-1 (ICAM-1) plays an important role in the recruitment of leukocytes to the endothelium, which causes inflammation and initiation of atherosclerosis. We have previously shown that endothelium-specific over-expression of class III deacetylase SIRT1 decreases atheroscler …
These reference sequences exist independently of genome builds. Explain. These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above. ...
Kit Component:- KN304792G1, Dpy19l1 gRNA vector 1 in pCas-Guide vector- KN304792G2, Dpy19l1 gRNA vector 2 in pCas-Guide vector- KN304792D, donor…
PLSCR3 (phospholipid scramblase 3 Scr3) is one of the superfamily of membrane-associated transcription regulators named Tubby-like protein (TULPs). medium LAQ824 by means of extracellular microvesicles (exosomes). Alternatively Scr3 expression didnt decrease as well as the secretion of Scr3 significantly?in 3T3 Swiss-albino fibroblasts (a parental cell-line of 3T3-L1) had not been increased by differentiation treatment. Overexpression of human being Scr3 during 3T3-L1 differentiation suppressed triacylglycerol build up and inhibited induction from the mRNAs lately stage pro-adipogenic transcription elements [CCAAT/enhancer-binding proteins α (C/EBPα) and peroxisome proliferator-activated receptor γ (PPARγ)] and X-box-binding proteins 1 (XBP1). Manifestation of early stage pro-adipogenic transcription elements (C/EBPβ and C/EBPδ) had not been considerably affected. These outcomes claim that Scr3 features as a poor regulator of adipogenesis in 3T3-L1 cells at a particular differentiation ...
May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane.
Taylor, SRJ, Gonzalez-Begne, M, Dewhurst, S, Chimini, G, Higgins, CF, Melvin, JE and Elliott, JI (2008) Sequential shrinkage and swelling underlie P2X(7)-stimulated lymphocyte phosphatidylserine exposure and death ...
APhA DrugInfoLine (ISSN 2162-3015) is a weekly publication of, and is owned and copyrighted by the American Pharmacists Association, the national professional society of pharmacists. Materials in APhA DrugInfoLine do not neccessarily represent the policy, recommendations, or endorsement of APhA. The publisher, authors, editors, reviewers, and contributors have taken care to ensure that the information contained in APhA DrugInfoLine is accurate and current; however, they shall have no ability to any person or entity with regard to claims, losses, or damage caused or alleged to be caused, directly or indirectly by use of any information contained in the publication. All decisions about drug therapy must be based on independent judgement of the clinician.. ...
Ano ano ba ang mga gamot na pampatigas - inda dadaesen Ang Lungsod - W2C - Web To Corpus. Ideal for treating acne spots directly.
Tmem18兔多克隆抗体(ab106597)可与小鼠, 大鼠, 人样本反应并经WB, IHC, ICC/IF实验严格验证。所有产品均提供质保服务,中国75%以上现货。
Increased numbers of eosinophils and mast cells in the bronchial mucosa are characteristic features in subjects with aspirin-sensitive asthma. Interleukin-5 (IL-5) and granulocyte-macrophage colony-stimulating factor (GM-CSF) are involved in the activation, maturation, and perpetuation of survival o …
Sluyter, R., Shemon, A. & Wiley, J. S. (2007). P2X7 receptor activation causes phosphatidylserine exposure in human erythrocytes. Biochemical and Biophysical Research Communications, 355 169-173 ...
P edseda poslaneck ho klubu ANO Jaroslav Falt nek z ejm p t rok nebude na sn mu obhajovat funkci prvn ho m stop edsedy hnut . Po sch zce s n m to televizi Nova ekl p edseda hnut a premi r Andrej Babi . D vodem je z ejm situace kolem brn nsk organizace ANO, kterou Falt nek zakl dal.
TMEM204兔多克隆抗体(ab113102)可与小鼠, 大鼠, 人样本反应并经WB, IHC, ICC, ICC/IF实验严格验证。所有产品均提供质保服务,中国75%以上现货。
This conclusion was supported by studies of IκBα protein turnover in the DLBCL cell lines. Because IκBα transcription is itself positively regulated by NF-κB activity (37, 38), the steady-state level of IκBα protein does not necessarily reflect the rate of phosphorylation by IKK and subsequent degradation. Indeed, full-length IκBα protein is found at comparable levels in our untreated cell lines (Fig. 3 A). However, when ABC DLBCL cell lines were treated with cycloheximide (CHX) to block new protein synthesis, the level of IκBα declined rapidly through phosphorylation and degradation (Fig. 3 A). By contrast, the IκBα level in SUDHL-6 was significantly more stable upon CHX treatment unless the cells were also stimulated by BCR cross-linking or by PMA and ionomycin treatment (Fig. 3 A). Lactacystin, a drug that inhibits degradation of ubiquitinated IκBα by proteasomes, prevented the CHX-induced loss of IκBα in resting ABC DLBCL cell lines and in stimulated SUDHL-6 cells (Fig. 3 ...
May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane.
In vitro PMA/ionomycin treatment for 5 h, which activates a signal transduction step down-stream of ZAP-70 and therefore equally activates SKG and BALB/c T cells, revealed that a significant fraction of LN CD4+ T cells from nonarthritic SKG mice in an SPF environment were producing IL-17A (hereafter IL-17), whereas SKG or BALB/c CD4-SP thymocytes or BALB/c CD4+ T cells were not (Fig. 1 A and Fig. S1, which is available at http://www.jem.org/cgi/content/full/jem.20062259/DC1). Such IL-17-producing SKG CD4+ T cells also produced at a single cell level TNF-α and IL-2, but not IFN-γ, IL-4, or IL-10, a profile distinct from Th1 or Th2 cells and similar to that of Th17 cells (Fig. 1 A; references 7-9). CD4+ T cells freshly prepared from nonarthritic SKG mice also actively transcribed IL-17 and IL-23R mRNA (Fig. 1 B). In arthritic SKG mice raised in a conventional environment, arthritic joints actively transcribed IL-17 mRNA, whereas nonarthritic ones did not (Fig. 1 C). Correspondingly, CD4+ T cells ...
w00t! after 6 long weeks my emag has returned from ano! I could not be happier with the finish :D Enough chit-chat... PICTURES! Before: http://i1046.photobucket.com/albums/b461/sQuid-Vision/0F07329E-3CBC-41F4-B2CF-A4E8531CA79B-7719-0000081BE189A8F7.jpg Broken Down for Ano:
COMUNICAÇÕES APRESENTADAS EM CONGRESSOS INTERNACIONAIS As Comunicações estão organizadas em duas partes. A primeira compreende o período de 1976-1995 e, no segundo, temos informações apresentadas, ano a ano, a partir de 1996.. ...
Unregulated increases in cellular Ca2+ homeostasis are a hallmark of pathophysiological conditions and a key trigger of cell death. Endothelial cells cultured under physiologic O2 conditions (5% O2) exhibit a reduced cytosolic Ca2+ response to stimulation. The mechanism for reduced plateau [Ca2+]i upon stimulation was due to increased sarco/endoplasmic reticulum Ca2+ ATPase (SERCA)-mediated reuptake rather than changes in Ca2+ influx capacity. Agonist-stimulated phosphorylation of the SERCA regulatory protein phospholamban was increased in cells cultured under 5% O2 Elevation of cytosolic and mitochondrial [Ca2+] and cell death after prolonged ionomycin treatment, as a model of Ca2+ overload, were lower when cells were cultured long-term under 5% compared with 18% O2 This protection was abolished by cotreatment with the SERCA inhibitor cyclopiazonic acid ...
6. CONCLUSIONS 1. We found that mast cell stimulation can induce PS externalization in the absence of secretory response. 2. We identified GPI-APs as molecules whose engagement can induce sustained and reversible non-apoptotic PS externalization. 3. GPI-AP-induced PS externalization was determined as non- apoptotic and distinct from the FceRl-induced PS externalization. 4. The effect of multiple triggering on PS externalization was additive and dependent on a tlpe of stimulus and cells engaged. 5. We identifred PLSCR1 as a molecule that becomes tyrosine phosphorylated in mast cells stimulated through GPI-APs. 6. We found that the PLSCR1 tyrosine phosphorylation is not associated with mast cell secretory response' and with GPI-AP- or FceRl-induced non-apoptotic PS externalization. 7. Using confocal microscopy and electron microscopy visualization of PLSCR1 in the course of mast cell activation we found that PLSCR1: (1) is not co-localized with extemalized PS, (2) is not co- localized with ...
The lack of understanding of AERD and its underlying cause has created a critical barrier in developing effective treatments for the condition. Many practitioners are unaware of AERD and this is often why it takes so long to connect all the pieces of the puzzle before the correct treatment can be offered, Dr. Jerschow said.. Additionally, the very act of diagnosing AERD is risky, since many patients will suffer an asthma attack in response to the standard oral-graded aspirin challenge. Dr. Jerschows study will compare the pharmacological effects (biochemical changes in the body) of aspirin in AERD patients to those of aspirin-tolerant asthmatics. Her protocol, conducted on 25-30 participants aged 25-62 (some of whom are referred by the Department of Otorhinolaryngology - Head & Neck Surgery), will use a low-dose aspirin challenge to avoid allergic reactions. The low-dose aspirin challenge begins by giving the patient a small amount of aspirin. This approach usually does not lead to a ...
Recombinant Human DPY30 protein is an Escherichia coli Full length protein 2 to 99 aa range, |=85% purity and validated in SDS-PAGE.
Effect of pharmacologic kinase inhibition on normal hematopoietic signaling. Having established a baseline of healthy signaling responses to a panel of stimuli, we examined cell type-specific pharmacologic effects of some well-characterized kinase inhibitors. These included the Janus kinase (JAK) I inhibitor and MAPK kinase (MEK) inhibitor U0126. Predictably, when combined respectively with G-CSF and PMA/Ionomycin treatments of human BM (figs. S8 and S9) reliable and specific inhibition, respectively, of STAT3 and ERK1/2 phosphorylation are observed, which is consistent with previously reported observations that used conventional single-cell analysis platforms (44). Although interesting results were obtained with these inhibitors, we expanded to focus on dasatinib, a clinically relevant small-molecule kinase inhibitor. Dasatinib was originally introduced as a second-line BCR-ABL kinase inhibitor for imatinib-resistant chronic myelogenous leukemia (CML) (45). Unlike imatinib, dasatinib is ...
Besides their use in inflammatory lung diseases the novel bisphosphonates with prolonged carbon chain may be used as treatment option for cystic fibrosis and atherosclerosis.,br,,br, ,b,Benefits:,/b,,br, ,ul, ,li,Neutral sphingomyelinase is not influenced,/li, ,li,First ex-vivo data in a PAF-induced edema rat model showed clear edema reduction by 50percent,/li, ,li,Inhibition of dexamethasone induced apoptosis was proven ,em,in vitro,/em,,/li, ,li,Substances can easily be synthesized in a one-step or two-step procedure,/li, ,li,Prolongation from 6 to 8 C-atoms shows 40times higher inhibition rate,/li, ,/ul, ,p,,strong,IP Rights,/strong,,br, EP Application (08/2009).,br, PCT Application (08/2010). ,br, A PCT application was filed in August 2010.,br, ,br, ,strong,Patent Owner,/strong,,br, Humboldt-Universität zu Berlin,br, Rheinisch-Westfälische,br, Technische Hochschule Aachen (RWTH),br,,br, ,b,Find the technology offer and contact details here: ,a ...
Ano ang Latter Rain Movement? Magkakaroon ba ng pagbubuhos ng mga mahimalang espiritwal na kaloob habang nalalapit ang mga huling araw?
Calcium ionophores are also ideal to induce capacitation. Adding heparin to capacitation inducing medium mimics the secretion ... Calcium chloride is added to facilitated the influx via of calcium cations. In animal models, Tyrode's albumin lactate pyruvate ...
Fenstein, MB; Fraser, C (1975). "Human platelet secretion and aggregation induced by calcium ionophores. Inhibition by PGE1 and ... IP receptors bind with ionophores that induce ADP and serotonin secretion. PGE1 inhibits the secretion of factors that ... 5-triphosphate signaling and the mobilization of calcium inside the cell for thromboxane A2. PGI2, prostacyclin, binds to IP ...
... however others have found it necessary to also use calcium ionophore treatment for fertilisation to be successful. Calcium ... "Mechanism of action of calcium ionophores on intact cells: ionophore-resistant cells". Membrane & Cell Biology. 13 (3): 357-368 ... It has been found that treating globozoospermia with ICSI along with oocyte activation by calcium ionophore (an ion carrier ... "A Successful Healthy Childbirth in a Case of Total Globozoospermia with Oocyte Activation by Calcium Ionophore". Journal of ...
Jamil, K.; White, I. G. (December 1981). "Induction of acrosomal reaction in sperm with ionophore A23187 and calcium". Archives ... as well as the more commonly used calcium ionophore A23187. This can be done to serve as a positive control when assessing the ...
... and calcium ionophore-induced arachidonic acid release". J. Biol. Chem. 278 (40): 38206-13. doi:10.1074/jbc.M304816200. PMID ... CERK was later confirmed to bind calmodulin in the presence of calcium, indicating the calmodulin first binds calcium and then ... A calcium-stimulated lipid kinase that co-purifies with brain synaptic vesicles". J. Biol. Chem. 264 (24): 14354-60. PMID ... CERK has also been found to participate in the calcium-dependent degranulation of mast cells. Sugiura M, Kono K, Liu H, ...
Lee AS (January 1987). "Coordinated regulation of a set of genes by glucose and calcium ionophores in mammalian cells". Trends ... This calcium then interacts with calcineurin and calcium/calmodulin-dependent kinases that in turn activate transcription ... Muscular contraction during exercise causes calcium to be released from the sarcoplasmic reticulum (SR), a specialized ER ... calcium that is stored bound to resident molecular chaperones and; redox buffers that maintain the oxidizing environment ...
Asaga H, Yamada M, Senshu T (February 1998). "Selective deimination of vimentin in calcium ionophore-induced apoptosis of mouse ...
Calcium Ionophore, Antibiotic A23187 and Calcium Ionophore A23187. It is produced at fermentation of Streptomyces ... The ionophore is used in laboratories to increase intracellular Ca2+ levels in intact cells. It also uncouples oxidative ... It also acts as a divalent cation ionophore, allowing these ions to cross cell membranes, which are usually impermeable to them ... of Topotecan Enhances Anticancer Efficacy in Murine and Human Xenograft Models Cancer Research Effect of calcium ionophore on ...
ARE-directed mRNA degradation is influenced by many exogenous factors, including phorbol esters, calcium ionophores, cytokines ...
Reiss, Hans-Dieter; Herth, Werner (1979). "Calcium ionophore A 23187 affects localized wall secretion infqu hoeegion of pollen ...
... is an ionophore and an antibiotic that binds calcium ions (Ca2+) in a ratio 1:1. It is produced by the bacterium ... Its β-diketone and carboxylic acid group form a chelate with calcium. It was extracted in 1978 and the complete structure was ... It is used in research to raise the intracellular calcium level (Ca2+) and as a research tool to understand Ca2+ transport ... 1979). "Structure of ionomycin - a novel diacidic polyether antibiotic having high affinity for calcium ions". Journal of the ...
"Epidermal growth factor induces p11 gene and protein expression and down-regulates calcium ionophore-induced arachidonic acid ... "Epidermal growth factor induces p11 gene and protein expression and down-regulates calcium ionophore-induced arachidonic acid ... S100 calcium-binding protein A10 (S100A10), also known as p11, is a protein that is encoded by the S100A10 gene in humans and ... Wu T, Angus CW, Yao XL, Logun C, Shelhamer JH (Jul 1997). "P11, a unique member of the S100 family of calcium-binding proteins ...
... may be artificially facilitated by calcium ionophores, something that is speculated to be useful in case of ... The slow block is through a biochemical mechanism triggered by a wave of calcium increase. The rise of calcium is both ... "Roscovitine in combination with calcium ionophore induces oocyte activation through reduction of M-phase promoting factor ... Sperm entry causes calcium release into the oocyte. In mammals, this is caused by the introduction of phospholipase C isoform ...
Saunders, M. J.; P. K. Hepler (1982). "Calcium ionophore A23187 stimulates cytokinin-like mitosis in Funaria". Science. 217 ( ... This calcium binding may account for the bulk of the observed extracellular current. The intracellular calcium gradient may ... He has shown that calcium ions and protons are essential for growth. The intracellular free calcium ions exist in a gradient ... The delay between the extracellular and intracellular calcium peaks indicates that calcium ions do not immediately enter the ...
The anergy in T cells can be induced by Ionomycin, the ionophore capable of raising intracellular concentration of calcium ions ... Conversely, Ca+II chelators such as EGTA can sequester Calcium ions making them unable to cause the anergy. Blocking of the ... Under this condition, calcium dependent phosphatase calcineurin removes phosphates from a transcriptional factor NFAT, which in ... On the contrary, TCR signalling without costimulatory receptors sufficiently activates only the calcium arm of the signalling ...
Interference from calcium, magnesium, or lithium, on the other hand, is weak enough that their presence in normal ... These electrodes are typical ion exchange resin membrane electrodes, using valinomycin, a potassium ionophore, as the ion ... calcium, magnesium, and lithium. The most significant interference with measurement of potassium concentration is from the ...
Sperm activation has been shown to be caused by calcium ionophores in vitro, progesterone released by nearby cumulus cells and ... The opening of CatSper channels is responsible for the influx of calcium. The sudden rise in calcium levels causes the ... A recent discovery links hyperactivation to a sudden influx of calcium ion into the tails. The whip-like tail (flagellum) of ... These channels are selective, allowing only calcium ions to pass. ...
... slow and inert in the reproductive tracts or storage areas of the female and are only activated by calcium ionophores in vitro ...
PCP-2 mRNA is regulated by phorbol myristate acetate (PMA) or calcium ionophore, okadaic acid, the Ras inhibitor manumycin, and ... down-regulation of gene expression by PMA and calcium ionophore in Jurkat T lymphoma cells". Biochem Biophys Res Commun. 231 (1 ...
... calcium channel agonists MeSH D27.505.519.562.249 - calcium channel blockers MeSH D27.505.519.562.374 - ionophores MeSH D27.505 ... calcium channel agonists MeSH D27.505.954.411.793.610 - nasal decongestants MeSH D27.505.954.411.918 - vasodilator agents MeSH ... calcium channel blockers MeSH D27.505.954.411.207 - cardioplegic solutions MeSH D27.505.954.411.222 - cardiotonic agents MeSH ...
... as well as the more commonly used calcium ionophore A23187. Birefringence microscopy, flow cytometry or fluorescence microscopy ... Once the two meet, a calcium influx occurs, causing a signaling cascade. The cortical granules inside the oocyte then fuse to ...
A23187 (Calcimycin, Calcium Ionophore). *Abamectin Abamectin. *Aigéad aibiteach Abietic acid. *Aigéad aicéiteach Acetic acid ...
The vibrating ion-selective microelectrode was first used in 1990 to measure calcium fluxes in various cells and tissues. The ... Only the specific ion permeates the ionophore, therefore the voltage readout is proportional to the ion concentration in the ... Some ions (such as calcium) and molecules (such as hydrogen peroxide) modulate targeted translocators to produce a current or ... ionophore) is tip-filled into a previously silanized (to prevent leakage) microelectrode. Also, the microelectrode vibrates at ...
... down-regulation of gene expression by PMA and calcium ionophore in Jurkat T lymphoma cells". Biochemical and Biophysical ... Flentke GR, Garic A, Amberger E, Hernandez M, Smith SM (July 2011). "Calcium-mediated repression of β-catenin and its ... Soluble inositol-(1,4,5)-trisphosphate triggers calcium to be released from the endoplasmic reticulum. This sudden increase in ... "Modification of the composition of polycystin-1 multiprotein complexes by calcium and tyrosine phosphorylation". Biochimica et ...
... such as calcium ionophore treatment, microinjection of calcium ions, or electrical stimulation. Treatment with cycloheximide, a ... The fertilization event causes intracellular calcium oscillations, and targeted degradation of cyclin B, a regulatory subunit ...
... and a lipid-soluble molecule that transports calcium ions (Ca2+) across the bilayer, also known as calcium ionophore. C1P was ... Inside the cell, S1P can induce calcium release independent of the S1PRs-the mechanism of which remains unknown. To date, the ... Tornquist, K. (February 2003). "Ceramide-1-phosphate increases intracellular free calcium concentrations in thyroid FRTL-5 ... and increase intracellular free calcium concentrations in thyroid cells. C1P also has known roles in vesicular trafficking, ...
... and did not affect the release of glutamate evoked by a calcium ionophore (ionomycin). A polypeptide (Tx3-3) present in the ... This effect was most likely due to interference with the entry of calcium through voltage-gated calcium channels, because PhTx3 ... The PhTx3 neurotoxin is a broad-spectrum calcium channel blocker that inhibits glutamate release, calcium uptake and also ... We compared the alterations produced by the Tx3-3 with the actions of toxins known to block calcium channels coupled to ...
Calcium Ionophore) Abamectine Abietic acid Acetic acid Acetylcholine Actin Actinomycin D Adenine Adenosmeme Adenosine ...
... greatly overproduce 15-hydroxyicosatetraenoic acid and eoxin C4 when challenged with arachidonic acid or calcium ionophore ...
... a novel diacidic polyether antibiotic having high affinity for calcium ions". Journal of the American Chemical Society. 101 (12 ... unlike carrier ionophores. Examples of channel-forming ionophores are gramicidin A and nystatin. Ionophores that transport ... Ionophores can be selective to a particular ion but may not be exclusive to it. Ionophores facilitate the transport of ions ... An ionophore (from Greek ion and -phore, "ion carrier") is a chemical species that reversibly binds ions. Many ionophores are ...
Calcium Scandium Titanium Vanadium Chromium Manganese Iron Cobalt Nickel Copper Zinc Gallium Germanium Arsenic Selenium Bromine ... carbohydrates and ionophore complexes.[218] ... calcium (1.5%), and aluminium (1.4%); with the remaining 1.2% ... Similar solutions are formed by the heavy divalent alkaline earth metals calcium, strontium, barium, as well as the divalent ... with calcium chloride typically added to lower the melting point of the mixture. The heavier alkali metals, however, is more ...
Hammarberg T, Reddy KV, Persson B, Rådmark O (2002). "Calcium binding to 5-lipoxygenase". Adv. Exp. Med. Biol. Advances in ... Rouzer CA, Kargman S (1988). "Translocation of 5-lipoxygenase to the membrane in human leukocytes challenged with ionophore ...
Mg2+ ions close certain types of calcium channels, which conduct positively charged calcium ions into neurons. With an excess ... Bulychev, A. A.; Vredenberg, W. J. (1976). "Effect of ionophores A-23187 and nigericin on the light induced redistribution of ... Too much magnesium may make it difficult for the body to absorb calcium.[citation needed] Not enough magnesium can lead to ... March 2008). "Magnesium, calcium, potassium, and sodium intakes and risk of stroke in male smokers". Arch. Intern. Med. 168 (5 ...
About 99% of mammals' mass are the elements carbon, nitrogen, calcium, sodium, chlorine, potassium, hydrogen, phosphorus, ... Like many antibiotics, monensin-A is an ionophore that tighlty bind Na+ (shown in yellow).[13] ... The most important ions are sodium, potassium, calcium, magnesium, chloride, phosphate, and bicarbonate. The maintenance of ... For example, muscle contraction depends upon the movement of calcium, sodium and potassium through ion channels in the cell ...
Calcium Scandium Titanium Vanadium Chromium Manganese Iron Cobalt Nickel Copper Zinc Gallium Germanium Arsenic Selenium Bromine ... like crown ethers and other ionophores, also have a high affinity for the sodium ion; derivatives of the alkalide Na− are ... As calcium is less electropositive than sodium, no calcium will be deposited at the cathode.[56] This method is less expensive ... Sodium-calcium alloys are by-products of the electrolytic production of sodium from a binary salt mixture of NaCl-CaCl2 and ...
... causes release of calcium from mitochondrial stores and prevents calcium re-uptake. This leads to free intracellular calcium ... In living cells, DNP acts as a proton ionophore, an agent that can shuttle protons (hydrogen cations) across biological ... Dantrolene inhibits calcium release from the sarcoplasmic reticulum which reduces intracellular calcium. The resulting muscle ...
Calcium-loading also leads to formation of phosphatidylserine:calcium:phosphate complexes in the plasma membrane mediated in ... Applying ionophores like valinomycin can create electrochemical gradients comparable to the gradients inside living cells. ... During normal calcification, a major influx of calcium and phosphate ions into the cells accompanies cellular apoptosis ( ... Thus, matrix vesicles convey to the extracellular matrix calcium, phosphate, lipids and the annexins which act to nucleate ...
Sperm activation has been shown to be caused by calcium ionophores in vitro, progesterone released by nearby cumulus cells and ... The opening of CatSper channels is responsible for the influx of calcium. The sudden rise in calcium levels causes the ... A recent discovery links hyperactivation to a sudden influx of calcium ion into the tails. The whip-like tail (flagellum) of ... These channels are selective, allowing only calcium ions to pass. ...
... down-regulation of gene expression by PMA and calcium ionophore in Jurkat T lymphoma cells". Biochemical and Biophysical ... 100 Calcium-Mediated Repression of b-Catenin and Its Transcriptional Signaling Mediates Neural Crest Cell Death in an Avian ... Este repentino incremento do calcio citoplasmático activa a proteína quinase dependente de Ca2+/calmodulina (CaMKII). A CaMKII ... O inositol (1,4,5)-trisfosfato soluble desencadea a liberación de calcio do retículo endoplasmático. ...
... is a member of a family of naturally occurring cyclic ionophores known as the macrotetrolide antibiotics. The other ... Vishwanath, C. K.; Shamala, N.; Easwaran, K. R. K.; Vijayan, M. (1983). "Structure of nonactin-calcium perchlorate, C40H64O12. ... This ion selectivity is seen in other macrocyclic ligands, such as the cyclic ionophore valinomycin, which is also an ... Due to this property, nonactin is also called "ammonium ionophore". During complexation, the nonactin backbone convolutes into ...
... which releases calcium. This leads to an increase in mitochondrial calcium, dropping its membrane potential. The pore ... a polypeptide from the Red Sea Moses sole with ionophore activity". FEBS Letters. 242 (1): 161-6. doi:10.1016/0014-5793(88) ...
ISBN 978-3-527-30673-2. Sperelakis, Nicholas; Sperelakis, Nick (January 11, 2012). "Chapter 4: Ionophores in Planar Lipid ... mainly calcium, potassium, sodium, magnesium, iron, and manganese) by weight. Wood also contains sulfur, chlorine, silicon, ...
It also localizes to the acrosomal membrane of the sperm, where it putatively mediates calcium ion transmembrane transport. The ... "The use of anti-VDAC2 antibody for the combined assessment of human sperm acrosome integrity and ionophore A23187-induced ... In mammalian cardiomyocytes, VDAC2 promotes mitochondrial transport of calcium ions in order to power cardiac contractions. In ... Cell Calcium. 49 (2): 136-43. doi:10.1016/j.ceca.2010.12.004. PMID 21241999. Alvira CM, Umesh A, Husted C, Ying L, Hou Y, Lyu ...
Among the essential metals that lead interacts with are calcium, iron, and zinc. High levels of calcium and iron tend to ... A specific ion-selective electrode has been developed based on the ionophore S,S'-methylenebis (N,N-diisobutyldithiocarbamate ... Acute cases may require the use of disodium calcium edetate, the calcium chelate, and the disodium salt of ... By mimicking calcium, lead can cross the blood-brain barrier. It degrades the myelin sheaths of neurons, reduces their numbers ...
Calcium Ionophore A23187 hemimagnesium salt for your research needs. Find product specific information including CAS, MSDS, ... Biochemicals and Reagents, Calcium Ionophores, Calcium Signaling Research Tools, Cell Biology, Cell Signaling and Neuroscience ... Calcium Ionophore A23187 hemimagnesium salt Synonym: A23187 hemimagnesium salt, Antibiotic A 23187 hemimagnesium salt, ... Interferes with Cell Membrane Permeability (Ionophores), Intracellular Calcium Signaling, Mechanism of Action, Spectrum of ...
Effect of calcium ionophores on early development in fucoid algae * * Hable Whitney E. HABLE Whitney E. ... Calcium buffer injections block fucoid egg development by facilitating calcium diffusion SPEKSNIJDER J. E. ... Pollen tube growth is coupled to the extracellular calcium ion flux and the intracellular calcium gradient:effect of BAPTA-type ... Pollen tube growth and the intracellular cytosolic calcium gradient oscillate in phase whike extracellular calcium influx is ...
In order to adapt the sensors for calcium ion measurements in biological fluids with sufficient selectivity and stability, ... plastic membrane coatings containing ionophores were applied. These functionalized ZnO nanorods sensors showed a high ... Studies on Calcium Ion Selectivity of ZnO Nanowire Sensors Using Ionophore Membrane Coatings,. Journal of Nanotechnology,. vol ... Studies on Calcium Ion Selectivity of ZnO Nanowire Sensors Using Ionophore Membrane Coatings. ...
Calcium ionophore IV , C52H100N2O3 , CID 601910 - structure, chemical names, physical and chemical properties, classification, ...
Calcium, Dietary. Ionophores. Calcium Ionophores. Bone Density Conservation Agents. Physiological Effects of Drugs. Membrane ... Drug: Ca Ionophore A23187 Sibling oocytes are to be randomized to artificially activated using Calcium ionophore in cases with ... Experimental: Ca ionophore Artificial activation of the oocytes using Calcium ionophore in the cases of severe male factor ... Can Calcium Ionophore Application Enhance the ICSI Outcomes in Severe Male Factor Infertility?. The safety and scientific ...
Inhibition of calcium ionophore (A23187) stimulated LTB4 formation in human neutrophils. ...
The effects of the calcium inonophore A 23187 on growing pollen tubes of Lilium longiflorumThunb. cv. Ace were investigated ... Borle, A.B., Studer, R.: Effects of calcium ionophores on the transport and distribution of calcium, in isolated cells and in ... Foreman, J.C., Mongar, J.L., Gomperts, B.D.: Calcium ionophores and movement of calcium ions following the physiological ... Calcium ionophore A 23187 affects localized wall secretion in the tip region of pollen tubes of Lilium longiflorum. ...
Oral-aboral ectoderm differentiation of sea urchin embryos is disrupted in response to calcium ionophore * * Akasaka Koji ...
Endothelium dependence of dilation of pial arterioles in mouse brain by calcium ionophore.. W I Rosenblum, G H Nelson ... We used calcium ionophore A-23187, another dilating agent known from in vitro studies of large arteries to be endothelium- ... Endothelium dependence of dilation of pial arterioles in mouse brain by calcium ionophore. ... Endothelium dependence of dilation of pial arterioles in mouse brain by calcium ionophore. ...
Calcium ionophore A23187 (188 ng/ml) was added to respective wells 48 h later. After a total 96-h culture period, cells were ... In the studies presented here we show that under serum-free conditions, LPS, TNF-α, and calcium ionophore (CI) A23187 are each ... 3 Abbreviations used in this paper: DC, dendritic cell; CI, calcium ionophore; CsA, cyclosporin A; Mφ-SFM, macrophage serum- ... Calcium ionophore-treated peripheral blood monocytes and dendritic cells rapidly display characteristics of activated dendritic ...
An Ionophore for Calcium Ions and the Stimulation of Histamine Secretion from Rat Peritoneal Mast Cells J. C. FOREMAN; J. C. ... J. C. FOREMAN, B. D. GOMPERTS, J. L. MONGAR; An Ionophore for Calcium Ions and the Stimulation of Histamine Secretion from Rat ... Activation of (arachidonyl) phosphatidylinositol turnover in rabbit neutrophils by the calcium ionophore A23187 Biochem J ( ... Limits to the early increase in free cytoplasmic calcium concentration during the mitogenic stimulation of lymphocytes Biochem ...
Transactivation of the grp78 promoter by malfolded proteins, glycosylation block, and calcium ionophore is mediated through a ... Transactivation of the grp78 promoter by malfolded proteins, glycosylation block, and calcium ionophore is mediated through a ... Transactivation of the grp78 promoter by malfolded proteins, glycosylation block, and calcium ionophore is mediated through a ... Transactivation of the grp78 promoter by malfolded proteins, glycosylation block, and calcium ionophore is mediated through a ...
This induction requires a continuous presence of the ionophore for over 2 h. Although extracellular Ca2+ is important for the ... The calcium ionophore A23187 can reversibly induce the expression of two glucose-regulated genes, p3C5 and p4A3. ... Calcium ionophore A23187 as a regulator of gene expression in mammalian cells. @article{Resendez1986CalciumIA, title={Calcium ... A calcium ionophore-inducible cellular promoter is highly active and has enhancerlike properties.. *Albert Yu-Min Lin, Shu ...
Calcium ionophore synergizes with bacterial lipopolysaccharides in activating macrophage arachidonic acid metabolism. A A ... A A Aderem, Z A Cohn; Calcium ionophore synergizes with bacterial lipopolysaccharides in activating macrophage arachidonic acid ... The Ca2+ ionophore A23187 (0.1 microM) triggered the rapid release of 20:4 metabolites from LPS-primed macrophages but not from ...
Contrasting effects of local anesthetics and a calcium ionophore. J Braun , J Braun ... The Ca2+ ionophore may affect capping of microfilament-dependent caps by producing a systemic activation of contractile ... Contrasting effects of local anesthetics and a calcium ionophore.. J Cell Biol 1 November 1978; 79 (2): 419-426. doi: https:// ... In the present study, the effects of the local anesthetic chlorpromazine and of the Ca2+ ionophore A23187 were compared. Both ...
The induction of intracellular calcium mobilization through the use of the calcium ionophore A23187 (0.1, 0.5, 1, and 2 μM) ... The induction of intracellular calcium mobilization through the use of the calcium ionophore A23187 (0.1, 0.5, 1, and 2 {\^I}¼M ... The induction of intracellular calcium mobilization through the use of the calcium ionophore A23187 (0.1, 0.5, 1, and 2 μM) ... The induction of intracellular calcium mobilization through the use of the calcium ionophore A23187 (0.1, 0.5, 1, and 2 μM) ...
416 Xenbase articles contain a reference to calcium ionophore according to textpresso ... Retrieved from "http://wiki.xenbase.org/xenwiki/index.php?title=Calcium_ionophore&oldid=43133" ...
... high quality calcium ionophore from Hello Bio, a trusted supplier for life science researchers worldwide ... Role of calcium ionophore A23187-induced activation of IkappaB kinase 2 in mast cells.. Hosokawa J et al (2013) Int Arch ... References for Calcium Ionophore A23187. *. Zn(2+), derived from cell preparation, partly attenuates Ca(2+)-dependent cell ... The following papers have cited the use of Calcium Ionophore A23187 (HB1000) from Hello Bio. If you have published a paper ...
... regulates Th2 responses and antibody production by promoting naïve calcium ionophore to differentiate into antibody-producing ...
Category: Calcium Ionophore. Cachexia affects nearly all cancer sufferers, with currently zero effective remedies.. Posted on ... Posted in Calcium IonophoreTagged Rabbit Polyclonal to BAX, Risedronic acid (Actonel) supplier ... Posted in Calcium IonophoreTagged Rabbit Polyclonal to MART-1., SVT-40776 (Tarafenacin) ...
... and the calcium ionophore, ionomycin. T cells were rendered competent as the result of a 30-min initial incubation with both ... and the calcium ionophore, ionomycin. T cells were rendered competent as the result of a 30-min initial incubation with both ... and the calcium ionophore, ionomycin. T cells were rendered competent as the result of a 30-min initial incubation with both ... and the calcium ionophore, ionomycin. T cells were rendered competent as the result of a 30-min initial incubation with both ...
Calcium indicators and ionophores. Find the best indicator and ionophore for imaging Ca2+ and cell function. ... potassium and calcium. Join researchers worldwide and find ion channel modulators for your research. ...
... cultured in calcium ionophore A23187, and cultured in ionophore plus calpain inhibitor E64. Lenses were cultured for 7 days, ... cultured in calcium ionophore A23187, and cultured in ionophore plus calpain inhibitor E64. Lenses were cultured for 7 days, ... cultured in calcium ionophore A23187, and cultured in ionophore plus calpain inhibitor E64. Lenses were cultured for 7 days, ... cultured in calcium ionophore A23187, and cultured in ionophore plus calpain inhibitor E64. Lenses were cultured for 7 days, ...
Parthenogenetic Activation of Porcine Oocytes by Calcium Ionophore A23187 S. Yin1, M. Hishinuma2, K. Hamana3, and J. Sekine2 S ... Abstract. This study was designated to clarify the influence of activation of porcine matured oocytes by calcium ionophore on ... The in vitro-matured oocytes were exposed to calcium ionophore at concentrations of 12.5, 25 or 50 μM for 3, 5, 7 or 9 min. The ... However, development to blastocyst was observed only in the oocytes treated with 25 μM ionophore for 3 and 5 min (3 and 4% of ...
Measurement of free cytosolic calcium concentration in single CHO-K1 cells 635805852410000000 ...
Relaxations to Calcium Ionophore, Acetylcholine, and SNP. Relaxations to calcium ionophore were significantly enhanced in the ... The enhancement of relaxations to calcium ionophore in arteries transduced with AdeNOS is a novel finding. Calcium ionophore ... The concentration response to calcium ionophore was repeated in the presence of the NOS enzyme inhibitor L-NAME (3×10−4 mol/L ... When the concentration response to calcium ionophore was repeated in the presence of the NOS enzyme inhibitor L-NAME (3×10−4 ...
... ... 1996). Increased Hypodense Eosinophils After Activation with PAF-Acether and Calcium Ionophore in Asthmatic Subjects. Journal ... Increased Hypodense Eosinophils After Activation with PAF-Acether and Calcium Ionophore in Asthmatic Subjects ...
Both calcium ionophores, A23187 and ionomycin, in conjunction with 12-O-tetradecanoylphorbol 13-acetate (TPA) mimicked the ... Tumor promoters in conjunction with calcium ionophores mimic antigenic stimulation by reactivation of alloantigen-primed murine ... In addition, Ca2+ ionophore and TPA induced IL 2 receptor expression and IL 2 secretion. The capacity of other phorbol esters ... In addition, the synergistic effect of Ca2+ ionophore and TPA was blocked by either a Ca2+ chelator (EGTA) or cAMP, which is ...
28 29 30 The calcium ionophore A23187 directly promotes entry of calcium across the endothelial cell membrane.29 In the present ... Calcium Ionophore A23187. Dilated vessels showed significantly diminished relaxations in comparison to nondilated and normal ... These studies suggest that for the calcium ionophore A23187 (10−7 to 10−5 mol/L), an endothelium-dependent vasorelaxant, ... The calcium ionophore A23187 produces vasorelaxation through an endothelium-dependent mechanism. This mechanism requires the ...
Ischemia induces partial loss of surface membrane polarity and accumulation of putative calcium ionophores. / Molitoris, Bruce ... Ischemia induces partial loss of surface membrane polarity and accumulation of putative calcium ionophores. Journal of Clinical ... title = "Ischemia induces partial loss of surface membrane polarity and accumulation of putative calcium ionophores", ... T1 - Ischemia induces partial loss of surface membrane polarity and accumulation of putative calcium ionophores ...
  • Ionophore A23187 stops tip growth, but not cytoplasmic streaming, in pollen tubes of Lilium longiflorum. (nii.ac.jp)
  • Using a series of 5' deletions, linker-scanning, and internal deletion mutations spanning a 100-bp region from -179 to -80, we correlate the cis-acting regulatory elements mediating the activation of grp78 by malfolded proteins, glycosylation block, and the calcium ionophore A23187. (asm.org)
  • The calcium ionophore A23187 can reversibly induce the expression of two glucose-regulated genes, p3C5 and p4A3. (semanticscholar.org)
  • Calcium ionophore A23187 induces expression of glucose-regulated genes and their heterologous fusion genes. (semanticscholar.org)
  • The Ca2+ ionophore A23187 (0.1 microM) triggered the rapid release of 20:4 metabolites from LPS-primed macrophages but not from cells not previously exposed to LPS. (rupress.org)
  • In the present study, the effects of the local anesthetic chlorpromazine and of the Ca2+ ionophore A23187 were compared. (rupress.org)
  • The induction of intracellular calcium mobilization through the use of the calcium ionophore A23187 (0.1, 0.5, 1, and 2 μM) resulted in a dose-dependent suppression of PA activity. (elsevier.com)
  • Zn(2+), derived from cell preparation, partly attenuates Ca(2+)-dependent cell death induced by A23187, calcium ionophore, in rat thymocytes. (hellobio.com)
  • Platelet particle formation by anti GPIIIa49-66 Ab, Ca2+ ionophore A23187, and phorbol myristate acetate is induced by reactive oxygen species and inhibited by dexamethasone blockade of platelet phospholipase A2, 12-lipoxygenase, and NADPH oxidase. (hellobio.com)
  • Role of calcium ionophore A23187-induced activation of IkappaB kinase 2 in mast cells. (hellobio.com)
  • The following papers have cited the use of Calcium Ionophore A23187 (HB1000) from Hello Bio. (hellobio.com)
  • Lenses from 4-week- old (young group) and 12-week-old (adult group) rats were divided into four subgroups: noncultured control, cultured control, cultured in calcium ionophore A23187, and cultured in ionophore plus calpain inhibitor E64. (elsevier.com)
  • Both calcium ionophores, A23187 and ionomycin, in conjunction with 12-O-tetradecanoylphorbol 13-acetate (TPA) mimicked the effect of antigen or interleukin 2 (IL 2) by inducing strong proliferative and alloantigen-specific cytotoxic responses. (bgu.ac.il)
  • Contractile responses of isolated arterial rings from each ICA were recorded after treatment with KCl, noradrenaline, serotonin, and prostaglandin F 2α , while relaxations in response to the calcium ionophore A23187 and papaverine were recorded after tonic contraction to noradrenaline had been established. (ahajournals.org)
  • The divalent-cation ionophores A23187 and ionomycin exert dose-dependent suppressive effects on the stimulatory actions of luteinizing hormone in ovarian cells invitro. (elsevier.com)
  • The in vitro stimulation of PBMC by phorbol ester and Ca 2+ ionophore ( A23187 ) results in a pronounced decrease in the calcineurin activity of cells derived from patients with SLE or from healthy controls. (bmj.com)
  • The Ca(2+) ionophore A23187 mimicked the effect of 3'-benzoylbenzoyl adenosine 5'-triphosphate suggesting that an intracellular Ca(2+) increase was sufficient to evoke MV shedding. (nih.gov)
  • This fast activity disappeared in the presence of the Ca(2+) ionophore A23187, was inhibited by vanadate or thapsigargin but not by oligomycin, suggesting that this ATP synthesis is due to the reversal functioning of the Ca(2+)-ATPase previously found on the disk membranes. (nih.gov)
  • Calcium ionophore A23187 (A23187) inhibited human parainfluenza virus type 2 (hPIV-2) replication in LLCMK2 cells and the inhibition was almost completely recovered by monoclonal antibody (mAb) against CD98 heavy chain (CD98HC). (ijsciences.com)
  • A highly specific radioreceptor assay (RRA) for leukotriene (LT) C 4 using BC3H-1 cell membrane as a source of LTC 4 receptors was developed and demonstrated its use in the measurement of calcium ionophore A23187-induced LTC 4 production from purified eosinophils, neutrophils, and mononuclear cells. (elsevier.com)
  • The recovery of the exogenously added LTC 4 with eosinophils was greater than 90% in both the presence and the absence of calcium ionophore A23187. (elsevier.com)
  • Low levels of cPLA 2 activity in the KG1a cell line could be activated by the cPLA 2 activator mellitin, or the calcium ionophore A23187. (jimmunol.org)
  • Calcium increased AR to levels comparable to those reached by intact spermatozoa treated with well known stimulants like LPC and A23187. (mcmaster.ca)
  • 10. The method of claim 3, wherein the calcium ionophore is an ionomycin calcium salt, A23187, or combinations thereof. (sumobrain.com)
  • Moreover, the BAPTA-induced alkalinization was still apparent after depletion of intracellular Ca 2+ using the Ca 2+ ionophore A23187 in Ca 2+ -free medium. (biologists.org)
  • Comparison with ionophore A23187 in rat liver mitochondria. (alfa.com)
  • A general method, employing arsenazo III in liposomes, for study of calcium ionophores: results with A23187 and prostaglandins. (harvard.edu)
  • Ionomycin is a Ca 2+ ionophore that is more effective than A23187 as a mobile ion carrier for Ca 2+ . (sigmaaldrich.com)
  • Incubation with a bivalent cation ionophore (A23187) led to a small and more variable increase in phosphatidylinositol labelling with 32Pi, which occurred whether or not Ca2+ was available in the extracellular medium: this was not accompanied by significant phosphatidylinositol breakdown. (biochemj.org)
  • Used to study effects of elevated intracellular calcium on mitochondrial function and intrinsic apoptosis. (sigmaaldrich.com)
  • It is suggested that the process of intracellular Calcium elevation is triggered by binding of the sperm to the oocyte oolemma and hence the fertilization starts (3). (clinicaltrials.gov)
  • A ) AzA increases cAMP concentrations ( n = 4), but not intracellular calcium ( n = 8) or inositol phosphate (IP) levels ( n = 4). (jci.org)
  • We found that Aβ 1-42 induces enhanced permeability, disruption of zonula occludin-1 (ZO-1) expression in the plasma membrane, and increased intracellular calcium and matrix metalloproteinase (MMP) secretion in cultured endothelial cells. (jneurosci.org)
  • Ionomycin free acid is a beneficial tool in research to raise intracellular calcium levels. (alfa.com)
  • Ionomycin is a selective calcium ionophore derived from S. conglobatus that mobilizes intracellular calcium stores. (biomol.com)
  • The focus of the current study is the fabrication and demonstration of ZnO nanorods on a relatively thin silver wire suitable for the sensing of calcium ions in aqueous chemical solutions. (hindawi.com)
  • Foreman, J.C., Mongar, J.L., Gomperts, B.D.: Calcium ionophores and movement of calcium ions following the physiological stimulus to a secretory process. (springer.com)
  • Veldhuis, JD & Klase, PA 1982, ' Role of calcium ions in the stimulatory actions of luteinizing hormone in isolated ovarian cells: Studies with divalent-cation ionophores ', Biochemical and Biophysical Research Communications , vol. 104, no. 2, pp. 603-610. (elsevier.com)
  • Klase, Patricia A. / Role of calcium ions in the stimulatory actions of luteinizing hormone in isolated ovarian cells : Studies with divalent-cation ionophores . (elsevier.com)
  • Ionophores can disrupt the membrane potential by conducting ions through a lipid membrane in the absence of a protein pore, and thus could exhibit cytotoxic properties. (agscientific.com)
  • When channels are active, the pores open, allowing charged particles - which, in the case of TRPM3, are sodium and calcium ions - to pass through, carrying tiny electrical currents. (elifesciences.org)
  • They lower the threshold for CaSR activation by extracellular calcium ions and diminish PARATHYROID HORMONE (PTH) release from parathyroid cells. (nih.gov)
  • Substances that bind to and sequester CALCIUM ions. (nih.gov)
  • Chemical agents that increase the permeability of CELL MEMBRANES to CALCIUM ions. (nih.gov)
  • however, higher concentrations of the ionophore (0.1, 0.5, and 1.0 μM) completely abolished PMA-stimulated PA activity. (elsevier.com)
  • The in vitro -matured oocytes were exposed to calcium ionophore at concentrations of 12.5, 25 or 50 μM for 3, 5, 7 or 9 min. (copernicus.org)
  • Inhibitory concentrations of these ionophores deplete total cell content of calcium, and also seem to suppress protein synthesis. (elsevier.com)
  • Together with a slow component which accounted for a constant rate of about 22 nmol ATP/min/mg of protein and which was due to the adenylate kinase activity, a fast component with a maximal activity of about 58 nmol ATP/min/mg of protein was measured at physiological calcium concentrations. (nih.gov)
  • It is used to measure local calcium ion concentrations in vivo. (harvard.edu)
  • N. Moins, P. Gachon, and P. Duchene-Marullaz, Effects of two monocarboxylic ionophores, grisorixin and alborixin, on cardiovascular functions and plasma cation concentrations in the anesthetized dog, J. Cardiovasc. (springer.com)
  • It is used to study the effects of calcium flux on endoplasmic reticulum (ER) stress, mitochondrial stress and intrinsic apoptosis mechanisms. (alfa.com)
  • Localization of membrane-associated calcium during development of fucoid algae using chlorotetracycline. (nii.ac.jp)
  • In order to adapt the sensors for calcium ion measurements in biological fluids with sufficient selectivity and stability, plastic membrane coatings containing ionophores were applied. (hindawi.com)
  • Regulation of membrane assembly during development of sarcoplasmic reticulum: the possible role of calcium. (semanticscholar.org)
  • Non-selective calcium ionophore which facilitates transport of Ca 2+ across the plasma membrane. (hellobio.com)
  • We propose that ischemia impairs the ability of cells to maintain surface membrane polarity, and also results in the accumulation of putative calcium ionophores. (elsevier.com)
  • The process of nonactin is to bind a metal ionophore and transport it across the cell membrane. (agscientific.com)
  • Magnesium ionophore III is used in solvent polymeric membrane electrodes for the assay of Mg2+ activities in blood serum in vitro. (agscientific.com)
  • and (3) a polymeric membrane comprising an ion-selective ionophore in electrochemical contact with the electrochemical reference. (google.es)
  • Also this antibiotic can be used to study the calcium transport across the plasma membrane. (alfa.com)
  • Uncoupling secretion and tip growth in lily pollen tubes: Evidence for the role of calcium in exocytosis. (nii.ac.jp)
  • In addition, Ca2+ ionophore and TPA induced IL 2 receptor expression and IL 2 secretion. (bgu.ac.il)
  • To determine whether the induction of this cytotoxic activity was mediated by a direct effect of Ca2+ ionophore and TPA on cytotoxic T (Tc) cells or was secondary to IL 2 secretion by activated helper T (Th) cells, we tested the effect of Ca2+ ionophore and TPA on isolated populations of cloned, alloantigen-specific Th and Tc cells. (bgu.ac.il)
  • IP receptors bind with ionophores that induce ADP and serotonin secretion. (wikipedia.org)
  • S. J. Suchard, F. A. Lattanzio Jr., R. W. Rubin, and B. C. Pressman, Stimulation of catecholamine secretion from cultured chromaffin cells by an ionophore - mediated rise in intracellular sodium, J. Cell. (springer.com)
  • Pressman, B.C.: Properties of ionophores with broad range cation selectivity. (springer.com)
  • Ionophore highly selective for Ca2+. (sigmaaldrich.com)
  • 6. The ion-selective electrode of claim 5 wherein the ion-selective ionophore is selected from a group consisting of crown ethers, crown ether derivatives, and mixtures thereof. (google.es)
  • 7. The ion-selective electrode of claim 6 wherein the ion-selective ionophore is 6,6-dibenzyl-14-crown-4 ether. (google.es)
  • 10. The ion-selective electrode of claim 9 wherein the ion-selective ionophore is selected from the group consisting of nonactin, monactin, and mixtures thereof. (google.es)
  • 11. The ion-selective electrode of claim 10 wherein the ion-selective ionophore is nonactin. (google.es)
  • 12. The ion-selective electrode of claim 1 wherein the concentration of the ion for which the ion-selective ionophore is selective is from about 0.1 mM to about 10 mM in the electrochemical reference. (google.es)
  • Therefore, tissue-selective calcium agonists have the potential to combat cardiac failure and endocrinological disorders. (nih.gov)
  • A class of drugs that act by selective inhibition of calcium influx through cellular membranes. (nih.gov)
  • Calcium ionophores are also ideal to induce capacitation. (wikipedia.org)
  • On the other hand, GDPbS, a G-protein inhibitor, was able of blocking the increase of AR induced by calcium. (mcmaster.ca)
  • adenylate cyclase activator, a phosphodiesterase (PDE) inhibitor, a Toll-like receptor ligand, a calcium ionophore, a protein kinase A (PKA) activator, a protein kinase C (PKC) activator, a beta2-adrenergic receptor agonist, an adenylate cyclase toxin, or combinations thereof. (sumobrain.com)
  • Tumor promoters in conjunction with calcium ionophores mimic antigenic stimulation by reactivation of alloantigen-primed murine T lymphocytes. (bgu.ac.il)
  • The stimulation of PBMC by phorbol ester and calcium ionophore decreased the calcineurin activity both in patients with SLE and in healthy controls. (bmj.com)
  • The mechanism is demonstrated to include stimulation of the ATP-binding cassette transporter A1 with subsequent activation of the calcium/calmodulin-dependent protein kinase kinase and the AMP-activated protein kinase pathway. (ahajournals.org)
  • Furthermore, both T and B cells lacking RelA showed marked reduction in proliferative responses to stimulation with Con A, anti-CD3, anti-CD3+anti-CD28, LPS, anti-IgM, and PMA+calcium ionophore. (rupress.org)
  • The relationship of calcium to receptor-controlled stimulation of phosphatidylinositol turnover. (biochemj.org)
  • Reed, P.W., Lardy, H.A.: Antibiotic A 23187 as a probe for the study of calcium and magnesium function in biological systems. (springer.com)
  • B. C. Pressman and N. T. deGuzman, Biological applications of ionophores: theory and practice, Ann. (springer.com)
  • B. C. Pressman, Biological applications of ionophores, Ann. (springer.com)
  • It is used as a research tool to raise the intracellular level of calcium, to study calcium transport across biological membranes, and to stimulate the intracellular production of cytokines. (biomol.com)
  • Partial activation of Fucus eggs with calcium onophores and low-sodium seawater. (nii.ac.jp)
  • The Ca2+ ionophore may affect capping of microfilament-dependent caps by producing a systemic activation of contractile proteins while chlorpromazine may act by disrupting a Ca2+-dependent link between surface complexes and the contractile proteins. (rupress.org)
  • This study was designated to clarify the influence of activation of porcine matured oocytes by calcium ionophore on in vitro development of the parthenotes. (copernicus.org)
  • The activation rate of the oocytes increased as concentration of ionophore decreased, being at 27-33 and 68-77 % for the oocytes treated with 50 and 12.5 μM ionophore, respectively. (copernicus.org)
  • We concluded that the activation treatment of the porcine oocytes with 12.5 μM ionophore for 5 min provided the highest develop-mental rate to morula, but this treatment is not sufficient to overcome a developmental block at the morula stage. (copernicus.org)
  • Because this mechanism appears to involve an increase in intracellular free Ca2+ concentration and activation of protein kinase C (PKC), we tested the effect of Ca2+ ionophores and PKC activators on alloantigen-specific primary mixed leukocyte culture cells. (bgu.ac.il)
  • The aim of this study was the evaluation of oocytes activation by addition of calcium ionophore in unfertilized oocytes in ICSI cycles. (bvsalud.org)
  • According to our results, oocytes activation with calcium ionophore had an acceptable fertilization rate, however high quality embryos remained low. (bvsalud.org)
  • Does the use of calcium ionophore during artificial oocyte activation demonstrate an effect on pregnancy rate? (embies.com)
  • To study the effect, if any, of calcium ionophore as a method of artificial oocyte activation (AOA) on pregnancy outcomes and fertilization rates. (embies.com)
  • Overexpression of SLAP-2 in B and T cell lines specifically impaired antigen receptor-mediated signaling events, including CD69 surface marker upregulation, nuclear factor of activated T cells (NFAT) promoter activation and calcium influx. (rupress.org)
  • Cochrane, D.E., Douglas, W.W.: Calcium-induced extrusion of secretory granules (exocytosis) in mast cells exposed to 48/80 or the ionophores A-23187 and X-537 A. Proc. (springer.com)
  • Usnic acid as calcium ionophore and mast cells stimulator. (physiciansweekly.com)
  • The highest cleavage rate (76%) and developmental rate to morula (41%) were observed in the oocytes treated with 12.5 μM ionophore for 5 min. (copernicus.org)
  • However, development to blastocyst was observed only in the oocytes treated with 25 μM ionophore for 3 and 5 min (3 and 4% of treated oocytes, respectively). (copernicus.org)
  • The unfertilized oocytes were washed with GIVF medium and were transferred to GIVF medium that contained 5 micro mol of calcium ionophore and were incubated for 10 minutes. (bvsalud.org)
  • Only 51 unfertilized oocytes with normal morphology were selected and were exposed to calcium ionophore. (bvsalud.org)
  • The effects of the calcium inonophore A 23187 on growing pollen tubes of Lilium longiflorum Thunb. (springer.com)
  • Tip growth is slowed down and stopped within 20 min after application of 5x10 -5 M ionophore A 23187. (springer.com)
  • Blevins, D.G., Barnett, N.M., Bottino, P.J.: The effects of calcium and the ionophore A 23187 on nodulation, nitrogen fixation and growth of soybeans. (springer.com)
  • In vivo studies on microtubules of the heliozoan axopodium using the ionophore A 23187 J. Cell Biol. (springer.com)
  • We used calcium ionophore A-23187, another dilating agent known from in vitro studies of large arteries to be endothelium-dependent, which moves calcium into endothelial cells rather than interacting with surface receptors. (ahajournals.org)
  • The suppression of T-cell proliferation was not restored by treatment with a calcium ionophore ( A 23187 ) or phorbol 12- myristate -13 acetate (PMA). (bvsalud.org)
  • A-23187 (10 μM), a calcium-ionophore positive control. (jci.org)
  • It was proved experimentally that magnesium ionophore III ligand can be considered in the nitrobenzene medium as a very effective receptor for Eu3+ and Am3+ cations. (agscientific.com)
  • Effects of Calcium and Calcium Ionophores and Channel Blockers of the Synthesis of Other Muscle Proteins 15. (routledge.com)
  • Calcium ionophore induced 73.0 ± 17.0 ng of LTC 4 production/10 6 eosinophils (n = 8), and this was about 20-40 times more than those from neutrophils and from mononuclear cells. (elsevier.com)
  • Transactivation of the grp78 promoter by malfolded proteins, glycosylation block, and calcium ionophore is mediated through a proximal region containing a CCAAT motif which interacts with CTF/NF-I. (asm.org)
  • Large changes in intracellular pH and calcium observed during heat shock are not responsible for the induction of heat shock proteins in Drosophila melanogaster. (semanticscholar.org)
  • Calcium ionophore synergizes with bacterial lipopolysaccharides in activating macrophage arachidonic acid metabolism. (rupress.org)
  • B. C. Pressman and M. Fahim, Pharmacology ana toxicology of the monovalent carboxylic ionophores, Ann. (springer.com)
  • Can Calcium Ionophore Application Enhance the ICSI Outcomes in Severe Male Factor Infertility? (clinicaltrials.gov)
  • It has been recorded to function by binding a metal ionophore and transporting it across cell membranes for release. (agscientific.com)
  • Magnesium ionophore III is an effective receptor for trivalent europium and americium. (agscientific.com)
  • In addition, the synergistic effect of Ca2+ ionophore and TPA was blocked by either a Ca2+ chelator (EGTA) or cAMP, which is thought to inhibit phosphatidylinositol metabolism. (bgu.ac.il)
  • 2012). CXCR5 is induced in antigen-activated T cells and dendritic cells and together with IL-10 and IL-21, regulates Th2 responses and antibody production by promoting naïve calcium ionophore to differentiate into antibody-producing plasma cells (Morita et al. (pentynoic-acid-stp-ester.com)
  • Conclusions We conclude that eNOS gene transfer to the adventitia alters vascular reactivity, as demonstrated by diminished contractile responses to phenylephrine and enhanced relaxations to calcium ionophore. (ahajournals.org)
  • F. A. Lattanzio, M. Fahim, R. Koos, M. Clark, and B. C. Pressman, The effects of prostaglandin release by ionophores on cardiovascular responses in the intact dog, Fed. (springer.com)
  • Brewbaker, J.L., Kwack, B.H.: The essential role of calcium ion in pollen germination and pollen tube growth. (springer.com)
  • The Role of Calcium in Signal Transduction Between the Cytoplasm and Nuclei. (routledge.com)
  • Calcium chloride is added to facilitated the influx via of calcium cations. (wikipedia.org)
  • Based on these reports, we hypothesized that TJs are altered by changes in Aβ-triggered cytosolic calcium influx and MMP expression. (jneurosci.org)
  • Agents that increase calcium influx into calcium channels of excitable tissues. (nih.gov)
  • Naohiko, T, Agrawal, DK & Townley, RG 1987, ' A specific radioreceptor assay for leukotriene C 4 and the measurement of calcium ionophore-induced leukotriene C 4 production from human leukocytes ', Journal of Pharmacological Methods , vol. 18, no. 4, pp. 327-333. (elsevier.com)
  • or peripheral blood leukocytes induced with calcium ionophore and mezerin. (reverso.net)
  • Small organic molecules that act as allosteric activators of the calcium sensing receptor (CaSR) in the PARATHYROID GLANDS and other tissues. (nih.gov)
  • Metallochrome indicator that changes color when complexed to the calcium ion under physiological conditions. (harvard.edu)
  • ou de leucocytes du sang provoqués avec du ionophore de calcium et de la mézerine. (reverso.net)
  • Nonactin is a cyclic ionophore that belongs to the macrotetrolide antibiotic family. (agscientific.com)
  • Transduction of calcium stress through interaction of the human transcription factor CBF with the proximal CCAAT regulatory element of the grp78/BiP promoter. (semanticscholar.org)
  • A calcium ionophore-inducible cellular promoter is highly active and has enhancerlike properties. (semanticscholar.org)
  • A gradient of cytoplasmic free calcium in growing rhizoid cells of Fucus serratus. (nii.ac.jp)
  • Borle, A.B., Studer, R.: Effects of calcium ionophores on the transport and distribution of calcium, in isolated cells and in liver and kidney slices. (springer.com)
  • In this report we also demonstrate and offer an explanation for two observed forms of the RelB protein and show that RelB can be induced in myeloid cells, either directly or indirectly, through a calcium-dependent and cyclosporin A-sensitive pathway. (jimmunol.org)
  • The resulting plasmid was linearized with Nru I and cotransfected with dl309 into 293 cells by calcium phosphate/DNA coprecipitation. (ahajournals.org)
  • Treatment of U937 cells with ionomycin, a calcium ionophore, also stimulates an increase in c-jun mRNA, but neither activates AP-1 enhancer activity nor stimulates differentiation of these cells. (elsevier.com)
  • Astrocytes are electrically nonexcitable cells that display increases in cytosolic calcium ion (Ca 2+ ) in response to various neurotransmitters and neuromodulators. (sciencemag.org)
  • Imaging experiments revealed that both mutant channels also increased the amount of calcium inside the cells. (elifesciences.org)
  • This could explain why the mutations cause disease, since abnormally high calcium levels can damage nerve cells. (elifesciences.org)
  • Increasing K + conductance using the specific ionophore valinomycin (10 μmol l -1 ) or removal of extracellular Cl - after an instant drop in AO fluorescence resulted in a reversal of luminal acidity. (biologists.org)
  • Like calcium, it will cause tetany (uncontrolled muscle contractions) when levels are too low in the extracellular fluids. (encyclopedia.com)
  • Ion channels may be a site of action for fast acting neurotransmitters such as GABA, with popular channels include sodium, potassium and calcium. (abcam.com)
  • This has lead to recommendations for baseline and post-treatment measurement of sodium, potassium, chloride, bicarbonate, calcium, phosphate, blood urea nitrogen, and creatinine in patients receiving oral sodium phosphate solution. (encyclopedia.com)
  • Ionomycin is an ionophore produced by the bacterium Streptomyces conglobatus . (sigmaaldrich.com)
  • This enzyme produces NO constitutively in a calcium calmodulin-dependent reaction. (ahajournals.org)
  • Adult lenses treated with ionophore also developed thin cortical opacity, but no nuclear opacity was observed, even though a large increase in the concentration of insoluble protein occurred. (elsevier.com)
  • R. K. Saini, R. K. Hester, P. Somani, and B. C. Pressman, Characterization of the coronary vasodilator and hemodynamic actions of monensin, a carboxylic ionophore, J. Cardiovasc. (springer.com)
  • N. T. deGuzman and B. C. Pressman, The inotropic effects of the calcium ionophore X-537A in the anesthetized dog, Circulation 44:1072 (1974). (springer.com)
  • Specifically, we sought to determine the effects of adventitial expression of eNOS on the response to a contractile agonist, phenylephrine, and vascular relaxations to calcium ionophore, a known stimulant of eNOS, in rabbit carotid arteries. (ahajournals.org)
  • Release of leukotrienes into the perfusate of calcium-ionophore stimulated rabbit lungs. (nih.gov)
  • Contrasting effects of local anesthetics and a calcium ionophore. (rupress.org)
  • In an attempt to further delineate the mechanism of action of CsA, we have examined its effects on T cell proliferation induced by the combination of the phorbol ester, phorbol 12,13-dibutyrate (PDB), and the calcium ionophore, ionomycin. (elsevier.com)
  • This kinase inhibits Gαq-phospholipase C-inositol 1,4,5-triphosphate signaling and the mobilization of calcium inside the cell for thromboxane A2. (wikipedia.org)