A multifunctional calcium-calmodulin-dependent protein kinase subtype that occurs as an oligomeric protein comprised of twelve subunits. It differs from other enzyme subtypes in that it lacks a phosphorylatable activation domain that can respond to CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASE KINASE.
A CALMODULIN-dependent enzyme that catalyzes the phosphorylation of proteins. This enzyme is also sometimes dependent on CALCIUM. A wide range of proteins can act as acceptor, including VIMENTIN; SYNAPSINS; GLYCOGEN SYNTHASE; MYOSIN LIGHT CHAINS; and the MICROTUBULE-ASSOCIATED PROTEINS. (From Enzyme Nomenclature, 1992, p277)
A heat-stable, low-molecular-weight activator protein found mainly in the brain and heart. The binding of calcium ions to this protein allows this protein to bind to cyclic nucleotide phosphodiesterases and to adenyl cyclase with subsequent activation. Thereby this protein modulates cyclic AMP and cyclic GMP levels.
A family of enzymes that catalyze the conversion of ATP and a protein to ADP and a phosphoprotein.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters.
A group of enzymes that are dependent on CYCLIC AMP and catalyze the phosphorylation of SERINE or THREONINE residues on proteins. Included under this category are two cyclic-AMP-dependent protein kinase subtypes, each of which is defined by its subunit composition.
A monomeric calcium-calmodulin-dependent protein kinase subtype that is expressed in a broad variety of mammalian cell types. Its expression is regulated by the action of CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASE KINASE. Several isoforms of this enzyme subtype are encoded by distinct genes.
Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.
Signal transduction mechanisms whereby calcium mobilization (from outside the cell or from intracellular storage pools) to the cytoplasm is triggered by external stimuli. Calcium signals are often seen to propagate as waves, oscillations, spikes, sparks, or puffs. The calcium acts as an intracellular messenger by activating calcium-responsive proteins.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Agents that inhibit PROTEIN KINASES.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A group of cyclic GMP-dependent enzymes that catalyze the phosphorylation of SERINE or THREONINE residues of proteins.
A group of enzymes that catalyzes the phosphorylation of serine or threonine residues in proteins, with ATP or other nucleotides as phosphate donors.
Toluenes in which one hydrogen of the methyl group is substituted by an amino group. Permitted are any substituents on the benzene ring or the amino group.
The rate dynamics in chemical or physical systems.
An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.
A mitosporic Ophiostomataceae fungal genus, whose species Sporothrix schenckii is a well-known animal pathogen. The conidia of this soil fungus may be inhaled causing a primary lung infection, or may infect independently via skin punctures.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Proteins which bind calmodulin. They are found in many tissues and have a variety of functions including F-actin cross-linking properties, inhibition of cyclic nucleotide phosphodiesterase and calcium and magnesium ATPases.
Established cell cultures that have the potential to propagate indefinitely.
A superfamily of PROTEIN-SERINE-THREONINE KINASES that are activated by diverse stimuli via protein kinase cascades. They are the final components of the cascades, activated by phosphorylation by MITOGEN-ACTIVATED PROTEIN KINASE KINASES, which in turn are activated by mitogen-activated protein kinase kinase kinases (MAP KINASE KINASE KINASES).
A species of ciliate protozoa. It is used in biomedical research.
A monomeric calcium-calmodulin-dependent protein kinase subtype that is primarily expressed in neuronal tissues; T-LYMPHOCYTES and TESTIS. The activity of this enzyme is regulated by its phosphorylation by CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASE KINASE.
A group of compounds that contain the structure SO2NH2.
Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS.
A specific protein kinase C inhibitor, which inhibits superoxide release from human neutrophils (PMN) stimulated with phorbol myristate acetate or synthetic diacylglycerol.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
A phenothiazine with actions similar to CHLORPROMAZINE. It is used as an antipsychotic and an antiemetic.
An intracellular signaling system involving the MAP kinase cascades (three-membered protein kinase cascades). Various upstream activators, which act in response to extracellular stimuli, trigger the cascades by activating the first member of a cascade, MAP KINASE KINASE KINASES; (MAPKKKs). Activated MAPKKKs phosphorylate MITOGEN-ACTIVATED PROTEIN KINASE KINASES which in turn phosphorylate the MITOGEN-ACTIVATED PROTEIN KINASES; (MAPKs). The MAPKs then act on various downstream targets to affect gene expression. In mammals, there are several distinct MAP kinase pathways including the ERK (extracellular signal-regulated kinase) pathway, the SAPK/JNK (stress-activated protein kinase/c-jun kinase) pathway, and the p38 kinase pathway. There is some sharing of components among the pathways depending on which stimulus originates activation of the cascade.
Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
An enzyme that phosphorylates myosin light chains in the presence of ATP to yield myosin-light chain phosphate and ADP, and requires calcium and CALMODULIN. The 20-kDa light chain is phosphorylated more rapidly than any other acceptor, but light chains from other myosins and myosin itself can act as acceptors. The enzyme plays a central role in the regulation of smooth muscle contraction.
Phosphotransferases that catalyzes the conversion of 1-phosphatidylinositol to 1-phosphatidylinositol 3-phosphate. Many members of this enzyme class are involved in RECEPTOR MEDIATED SIGNAL TRANSDUCTION and regulation of vesicular transport with the cell. Phosphatidylinositol 3-Kinases have been classified both according to their substrate specificity and their mode of action within the cell.
A mitogen-activated protein kinase subfamily that regulates a variety of cellular processes including CELL GROWTH PROCESSES; CELL DIFFERENTIATION; APOPTOSIS; and cellular responses to INFLAMMATION. The P38 MAP kinases are regulated by CYTOKINE RECEPTORS and can be activated in response to bacterial pathogens.
A dsRNA-activated cAMP-independent protein serine/threonine kinase that is induced by interferon. In the presence of dsRNA and ATP, the kinase autophosphorylates on several serine and threonine residues. The phosphorylated enzyme catalyzes the phosphorylation of the alpha subunit of EUKARYOTIC INITIATION FACTOR-2, leading to the inhibition of protein synthesis.
A phorbol ester found in CROTON OIL with very effective tumor promoting activity. It stimulates the synthesis of both DNA and RNA.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A cytoplasmic serine threonine kinase involved in regulating CELL DIFFERENTIATION and CELLULAR PROLIFERATION. Overexpression of this enzyme has been shown to promote PHOSPHORYLATION of BCL-2 PROTO-ONCOGENE PROTEINS and chemoresistance in human acute leukemia cells.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
A proline-directed serine/threonine protein kinase which mediates signal transduction from the cell surface to the nucleus. Activation of the enzyme by phosphorylation leads to its translocation into the nucleus where it acts upon specific transcription factors. p40 MAPK and p41 MAPK are isoforms.
Voltage-dependent cell membrane glycoproteins selectively permeable to calcium ions. They are categorized as L-, T-, N-, P-, Q-, and R-types based on the activation and inactivation kinetics, ion specificity, and sensitivity to drugs and toxins. The L- and T-types are present throughout the cardiovascular and central nervous systems and the N-, P-, Q-, & R-types are located in neuronal tissue.
A family of calcium/calmodulin-dependent PROETIN-SERINE-THREONINE KINASES. They are ubiquitously expressed in adult and embryonic mammalian tissues, and their functions are tightly related to the early stages of eukaryotic programmed cell death.
Specific enzyme subunits that form the active sites of the type I and type II cyclic-AMP protein kinases. Each molecule of enzyme contains two catalytic subunits.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Guanosine cyclic 3',5'-(hydrogen phosphate). A guanine nucleotide containing one phosphate group which is esterified to the sugar moiety in both the 3'- and 5'-positions. It is a cellular regulatory agent and has been described as a second messenger. Its levels increase in response to a variety of hormones, including acetylcholine, insulin, and oxytocin and it has been found to activate specific protein kinases. (From Merck Index, 11th ed)
The body of a fungus which is made up of HYPHAE.
A group of enzymes removing the SERINE- or THREONINE-bound phosphate groups from a wide range of phosphoproteins, including a number of enzymes which have been phosphorylated under the action of a kinase. (Enzyme Nomenclature, 1992)
A serine-threonine protein kinase family whose members are components in protein kinase cascades activated by diverse stimuli. These MAPK kinases phosphorylate MITOGEN-ACTIVATED PROTEIN KINASES and are themselves phosphorylated by MAP KINASE KINASE KINASES. JNK kinases (also known as SAPK kinases) are a subfamily.
A 44-kDa extracellular signal-regulated MAP kinase that may play a role the initiation and regulation of MEIOSIS; MITOSIS; and postmitotic functions in differentiated cells. It phosphorylates a number of TRANSCRIPTION FACTORS; and MICROTUBULE-ASSOCIATED PROTEINS.
A cyclic AMP-dependent protein kinase subtype primarily found in particulate subcellular fractions. They are tetrameric proteins that contain two catalytic subunits and two type II-specific regulatory subunits.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
A ubiquitously expressed protein kinase that is involved in a variety of cellular SIGNAL PATHWAYS. Its activity is regulated by a variety of signaling protein tyrosine kinase.
A group of compounds with the heterocyclic ring structure of benzo(c)pyridine. The ring structure is characteristic of the group of opium alkaloids such as papaverine. (From Stedman, 25th ed)
An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
A subgroup of mitogen-activated protein kinases that activate TRANSCRIPTION FACTOR AP-1 via the phosphorylation of C-JUN PROTEINS. They are components of intracellular signaling pathways that regulate CELL PROLIFERATION; APOPTOSIS; and CELL DIFFERENTIATION.
N-(N-(N(2)-(N-(N-(N-(N-D-Alanyl L-seryl)-L-threonyl)-L-threonyl) L-threonyl)-L-asparaginyl)-L-tyrosyl) L-threonine. Octapeptide sharing sequence homology with HIV envelope protein gp120. It is potentially useful as antiviral agent in AIDS therapy. The core pentapeptide sequence, TTNYT, consisting of amino acids 4-8 in peptide T, is the HIV envelope sequence required for attachment to the CD4 receptor.
A non-essential amino acid occurring in natural form as the L-isomer. It is synthesized from GLYCINE or THREONINE. It is involved in the biosynthesis of PURINES; PYRIMIDINES; and other amino acids.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
A chelating agent relatively more specific for calcium and less toxic than EDETIC ACID.
Intracellular signaling protein kinases that play a signaling role in the regulation of cellular energy metabolism. Their activity largely depends upon the concentration of cellular AMP which is increased under conditions of low energy or metabolic stress. AMP-activated protein kinases modify enzymes involved in LIPID METABOLISM, which in turn provide substrates needed to convert AMP into ATP.
A cyclic GMP-dependent protein kinase subtype that is expressed in SMOOTH MUSCLE tissues and plays a role in regulation of smooth muscle contraction. Two isoforms, PKGIalpha and PKGIbeta, of the type I protein kinase exist due to alternative splicing of its mRNA.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Proteins prepared by recombinant DNA technology.
The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.
A type I cAMP-dependent protein kinase regulatory subunit that plays a role in confering CYCLIC AMP activation of protein kinase activity. It has a lower affinity for cAMP than the CYCLIC-AMP-DEPENDENT PROTEIN KINASE RIBETA SUBUNIT.
An aspect of protein kinase (EC 2.7.1.37) in which serine residues in protamines and histones are phosphorylated in the presence of ATP.
Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
The relationship between the dose of an administered drug and the response of the organism to the drug.
Calcium compounds used as food supplements or in food to supply the body with calcium. Dietary calcium is needed during growth for bone development and for maintenance of skeletal integrity later in life to prevent osteoporosis.
A PROTEIN-TYROSINE KINASE family that was originally identified by homology to the Rous sarcoma virus ONCOGENE PROTEIN PP60(V-SRC). They interact with a variety of cell-surface receptors and participate in intracellular signal transduction pathways. Oncogenic forms of src-family kinases can occur through altered regulation or expression of the endogenous protein and by virally encoded src (v-src) genes.
A curved elevation of GRAY MATTER extending the entire length of the floor of the TEMPORAL HORN of the LATERAL VENTRICLE (see also TEMPORAL LOBE). The hippocampus proper, subiculum, and DENTATE GYRUS constitute the hippocampal formation. Sometimes authors include the ENTORHINAL CORTEX in the hippocampal formation.
A protein kinase C subtype that was originally characterized as a CALCIUM-independent, serine-threonine kinase that is activated by PHORBOL ESTERS and DIACYLGLYCEROLS. It is targeted to specific cellular compartments in response to extracellular signals that activate G-PROTEIN-COUPLED RECEPTORS; TYROSINE KINASE RECEPTORS; and intracellular protein tyrosine kinase.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
PKC beta encodes two proteins (PKCB1 and PKCBII) generated by alternative splicing of C-terminal exons. It is widely distributed with wide-ranging roles in processes such as B-cell receptor regulation, oxidative stress-induced apoptosis, androgen receptor-dependent transcriptional regulation, insulin signaling, and endothelial cell proliferation.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Protein kinases that catalyze the PHOSPHORYLATION of TYROSINE residues in proteins with ATP or other nucleotides as phosphate donors.
The sum of the weight of all the atoms in a molecule.
A cyclic GMP-dependent protein kinase subtype that is expressed predominantly in INTESTINES, BRAIN, and KIDNEY. The protein is myristoylated on its N-terminus which may play a role its membrane localization.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
Elements of limited time intervals, contributing to particular results or situations.
Transport proteins that carry specific substances in the blood or across cell membranes.
The muscle tissue of the HEART. It is composed of striated, involuntary muscle cells (MYOCYTES, CARDIAC) connected to form the contractile pump to generate blood flow.

Nitric oxide stimulates the stress-activated protein kinase p38 in rat renal mesangial cells. (1/6323)

Nitric oxide (NO) has gained increased attention as a diffusible universal messenger that plays a crucial role in the pathogenesis of inflammatory and autoimmune diseases. Recently, we reported that exogenous NO is able to activate the stress-activated protein kinase (SAPK) cascade in mesangial cells. Here, we demonstrate that exposure of glomerular mesangial cells to compounds releasing NO, including spermine-NO and (Z)-1- (N-methyl-N-[6-(N-methylammoniohexyl)amino]diazen)-1-ium-1,2-diolate (MAHMA-NO), results in an activation of the stress-activated p38-mitogen-activated protein kinase (p38-MAPK) cascade as measured by the phosphorylation of the activator of transcription factor-2 (ATF2) in an immunocomplex kinase assay. Activation of the p38-MAPK cascade by a short stimulation (10 min) with the NO donor MAHMA-NO causes a large increase in ATF2 phosphorylation that is several times greater than that observed after stimulation with interleukin-1beta, a well-known activator of the p38-MAPK pathway. Time course studies reveal that MAHMA-NO causes rapid and maximal activation of p38-MAPK after 10 min of stimulation and that activation declines to basal levels within 60 min. The longer-lived NO donor spermine-NO causes a comparable rapid activation of the p38-MAPK pathway; however, the increased activation state of p38-MAPK was maintained for several hours before control values were reattained after 24 h of stimulation. Furthermore, the NO donors also activated the classical extracellular signal-regulated kinase (ERK) p44-MAPK cascade as shown by phosphorylation of the specific substrate cytosolic phospholipase A2 in an immunocomplex kinase reaction. Both MAHMA-NO and spermine-NO cause a rapid activation of p44-MAPK after 10 min of stimulation. Interestingly, there is a second delayed peak of p44-MAPK activation after 4-24 h of stimulation with NO donors. These results suggest that there is a differential activation pattern for stress-activated and mitogen-activated protein kinases by NO and that the integration of these signals may lead to specific cell responses.  (+info)

A Drosophila TNF-receptor-associated factor (TRAF) binds the ste20 kinase Misshapen and activates Jun kinase. (2/6323)

Two families of protein kinases that are closely related to Ste20 in their kinase domain have been identified - the p21-activated protein kinase (Pak) and SPS1 families [1-3]. In contrast to Pak family members, SPS1 family members do not bind and are not activated by GTP-bound p21Rac and Cdc42. We recently placed a member of the SPS1 family, called Misshapen (Msn), genetically upstream of the c-Jun amino-terminal (JNK) mitogen-activated protein (MAP) kinase module in Drosophila [4]. The failure to activate JNK in Drosophila leads to embryonic lethality due to the failure of these embryos to stimulate dorsal closure [5-8]. Msn probably functions as a MAP kinase kinase kinase kinase in Drosophila, activating the JNK pathway via an, as yet, undefined MAP kinase kinase kinase. We have identified a Drosophila TNF-receptor-associated factor, DTRAF1, by screening for Msn-interacting proteins using the yeast two-hybrid system. In contrast to the mammalian TRAFs that have been shown to activate JNK, DTRAF1 lacks an amino-terminal 'Ring-finger' domain, and overexpression of a truncated DTRAF1, consisting of only its TRAF domain, activates JNK. We also identified another DTRAF, DTRAF2, that contains an amino-terminal Ring-finger domain. Msn specifically binds the TRAF domain of DTRAF1 but not that of DTRAF2. In Drosophila, DTRAF1 is thus a good candidate for an upstream molecule that regulates the JNK pathway by interacting with, and activating, Msn. Consistent with this idea, expression of a dominant-negative Msn mutant protein blocks the activation of JNK by DTRAF1. Furthermore, coexpression of Msn with DTRAF1 leads to the synergistic activation of JNK. We have extended some of these observations to the mammalian homolog of Msn, Nck-interacting kinase (NIK), suggesting that TRAFs also play a critical role in regulating Ste20 kinases in mammals.  (+info)

Alzheimer's disease: clues from flies and worms. (3/6323)

Presenilin mutations give rise to familial Alzheimer's disease and result in elevated production of amyloid beta peptide. Recent evidence that presenilins act in developmental signalling pathways may be the key to understanding how senile plaques, neurofibrillary tangles and apoptosis are all biochemically linked.  (+info)

Activation of c-Abl tyrosine kinase requires caspase activation and is not involved in JNK/SAPK activation during apoptosis of human monocytic leukemia U937 cells. (4/6323)

Genotoxic stress triggers the activation of several sensor molecules, such as p53, JNK1/SAPK and c-Abl, and occasionally promotes the cells to apoptosis. We previously reported that JNK1/SAPK regulates genotoxic stress-induced apoptosis in p53-negative U937 cells by activating caspases. c-Abl is expected to act upstream of JNK1/SAPK activation upon treatment with genotoxic stressors, but its involvement in apoptosis development is still unclear. We herein investigated the kinase activities of c-Abl and JNK1/SAPK during apoptosis elicited by genotoxic anticancer drugs and tumor necrosis factor (TNF) in U937 cells and their apoptosis-resistant variant UK711 cells. We found that the activation of JNK1/SAPK and c-Abl correlated well with apoptosis development in these cell lines. Unexpectedly, however, the JNK1/SAPK activation preceded the c-Abl activation. Moreover, the caspase inhibitor Z-Asp suppressed c-Abl activation and the onset of apoptosis but not the JNK1/SAPK activation. Interestingly, c-Abl tyrosine kinase inhibition by CGP 57148 reduced apoptosis without interfering with JNK1/SAPK activation. These results indicate that c-Abl acts not upstream of JNK1/ SAPK but downstream of caspases during the development of p53-independent apoptosis and is possibly involved in accelerating execution of the cell death pathway.  (+info)

Growth inhibition of breast cancer cells by Grb2 downregulation is correlated with inactivation of mitogen-activated protein kinase in EGFR, but not in ErbB2, cells. (5/6323)

Increased breast cancer growth has been associated with increased expression of epidermal growth factor receptor (EGFR) and ErbB2 receptor tyrosine kinases (RTKs). Upon activation, RTKs may transmit their oncogenic signals by binding to the growth factor receptor bound protein-2 (Grb2), which in turn binds to SOS and activates the Ras/Raf/MEK/mitogen-activated protein (MAP) kinase pathway. Grb2 is important for the transformation of fibroblasts by EGFR and ErbB2; however, whether Grb2 is also important for the proliferation of breast cancer cells expressing these RTKs is unclear. We have used liposomes to deliver nuclease-resistant antisense oligodeoxynucleotides (oligos) specific for the GRB2 mRNA to breast cancer cells. Grb2 protein downregulation could inhibit breast cancer cell growth; the degree of growth inhibition was dependent upon the activation and/or endogenous levels of the RTKs. Grb2 inhibition led to MAP kinase inactivation in EGFR, but not in ErbB2, breast cancer cells, suggesting that different pathways might be used by EGFR and ErbB2 to regulate breast cancer growth.  (+info)

The MAP kinase ERK2 inhibits the cyclic AMP-specific phosphodiesterase HSPDE4D3 by phosphorylating it at Ser579. (6/6323)

The extracellular receptor stimulated kinase ERK2 (p42(MAPK))-phosphorylated human cAMP-specific phosphodiesterase PDE4D3 at Ser579 and profoundly reduced ( approximately 75%) its activity. These effects could be reversed by the action of protein phosphatase PP1. The inhibitory state of PDE4D3, engendered by ERK2 phosphorylation, was mimicked by the Ser579-->Asp mutant form of PDE4D3. In COS1 cells transfected to express PDE4D3, challenge with epidermal growth factor (EGF) caused the phosphorylation and inhibition of PDE4D3. This effect was blocked by the MEK inhibitor PD98059 and was not apparent using the Ser579-->Ala mutant form of PDE4D3. Challenge of HEK293 and F442A cells with EGF led to the PD98059-ablatable inhibition of endogenous PDE4D3 and PDE4D5 activities. EGF challenge of COS1 cells transfected to express PDE4D3 increased cAMP levels through a process ablated by PD98059. The activity of the Ser579-->Asp mutant form of PDE4D3 was increased by PKA phosphorylation. The transient form of the EGF-induced inhibition of PDE4D3 is thus suggested to be due to feedback regulation by PKA causing the ablation of the ERK2-induced inhibition of PDE4D3. We identify a novel means of cross-talk between the cAMP and ERK signalling pathways whereby cell stimuli that lead to ERK2 activation may modulate cAMP signalling.  (+info)

p50(cdc37) acting in concert with Hsp90 is required for Raf-1 function. (7/6323)

Genetic screens in Drosophila have identified p50(cdc37) to be an essential component of the sevenless receptor/mitogen-activated kinase protein (MAPK) signaling pathway, but neither the function nor the target of p50(cdc37) in this pathway has been defined. In this study, we examined the role of p50(cdc37) and its Hsp90 chaperone partner in Raf/Mek/MAPK signaling biochemically. We found that coexpression of wild-type p50(cdc37) with Raf-1 resulted in robust and dose-dependent activation of Raf-1 in Sf9 cells. In addition, p50(cdc37) greatly potentiated v-Src-mediated Raf-1 activation. Moreover, we found that p50(cdc37) is the primary determinant of Hsp90 recruitment to Raf-1. Overexpression of a p50(cdc37) mutant which is unable to recruit Hsp90 into the Raf-1 complex inhibited Raf-1 and MAPK activation by growth factors. Similarly, pretreatment with geldanamycin (GA), an Hsp90-specific inhibitor, prevented both the association of Raf-1 with the p50(cdc37)-Hsp90 heterodimer and Raf-1 kinase activation by serum. Activation of Raf-1 via baculovirus coexpression with oncogenic Src or Ras in Sf9 cells was also strongly inhibited by dominant negative p50(cdc37) or by GA. Thus, formation of a ternary Raf-1-p50(cdc37)-Hsp90 complex is crucial for Raf-1 activity and MAPK pathway signaling. These results provide the first biochemical evidence for the requirement of the p50(cdc37)-Hsp90 complex in protein kinase regulation and for Raf-1 function in particular.  (+info)

Activation of c-Jun N-terminal kinase 1 by UV irradiation is inhibited by wortmannin without affecting c-iun expression. (8/6323)

Activation of c-Jun N-terminal kinases (JNKs)/stress-activated protein kinases is an early response of cells upon exposure to DNA-damaging agents. JNK-mediated phosphorylation of c-Jun is currently understood to stimulate the transactivating potency of AP-1 (e.g., c-Jun/c-Fos; c-Jun/ATF-2), thereby increasing the expression of AP-1 target genes. Here we show that stimulation of JNK1 activity is not a general early response of cells exposed to genotoxic agents. Treatment of NIH 3T3 cells with UV light (UV-C) as well as with methyl methanesulfonate (MMS) caused activation of JNK1 and an increase in c-Jun protein and AP-1 binding activity, whereas antineoplastic drugs such as mafosfamide, mitomycin C, N-hydroxyethyl-N-chloroethylnitrosourea, and treosulfan did not elicit this response. The phosphatidylinositol 3-kinase inhibitor wortmannin specifically blocked the UV-stimulated activation of JNK1 but did not affect UV-driven activation of extracellular regulated kinase 2 (ERK2). To investigate the significance of JNK1 for transactivation of c-jun, we analyzed the effect of UV irradiation on c-jun expression under conditions of wortmannin-mediated inhibition of UV-induced stimulation of JNK1. Neither the UV-induced increase in c-jun mRNA, c-Jun protein, and AP-1 binding nor the activation of the collagenase and c-jun promoters was affected by wortmannin. In contrast, the mitogen-activated protein kinase/ERK kinase inhibitor PD98056, which blocked ERK2 but not JNK1 activation by UV irradiation, impaired UV-driven c-Jun protein induction and AP-1 binding. Based on the data, we suggest that JNK1 stimulation is not essential for transactivation of c-jun after UV exposure, whereas activation of ERK2 is required for UV-induced signaling leading to elevated c-jun expression.  (+info)

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Rabbit polyclonal BCKDH kinase antibody validated for WB, IHC and tested in Human and Mouse. Referenced in 1 publication. Immunogen corresponding to…
Recombinant full-length human CAMK4 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CAMK4 is a multifunctional serine/threonine protein kinase and a member of Ca2+/calmodulin-dependent protein kinase family.
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Recombinant Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4) Protein (His tag). Spezies: Human. Quelle: Escherichia coli (E. coli). Jetzt Produkt ABIN668012 bestellen.
TY - JOUR. T1 - Mutational analysis of Ca2+-independent autophosphorylation of calcium/calmodulin-dependent protein kinase II. AU - Mukherji, Sucheta. AU - Soderling, Thomas. PY - 1995/6/9. Y1 - 1995/6/9. N2 - Previous studies with synthetic peptides indicate that residues 290-309, corresponding to the calmodulin (CaM)-binding domain of Ca2+/CaM-dependent protein kinase II interact with the catalytic core of the enzyme as a pseudosubstrate (Colbran, R. J., Smith, M. K., Schworer, C. M., Fong, Y. L., and Soderling, T. R. (1989) J. Biol. Chem. 264, 4800-4804). In the present study, we attempted to locate the pseudosubstrate motif by generation or removal of potential substrate recognition sequences (RXXS/T) at selected positions using site-directed mutagenesis. Based on previous results, Arg297, Thr305/306, and Ser314 were selected as key residues. Single mutations such as N294S, K300S, A302R, A309R, and R311A were expressed, purified, and characterized. Several of the mutants exhibited decreased ...
Olomoucine is known as a cyclin-dependent kinase inhibitor. We found that olomoucine blocked insulins ability to stimulate glucose transport. It did so without affecting the activity of known insulin signaling proteins. To identify the olomoucine-sensitive kinase(s), we prepared analogs that could be immobilized to an affinity resin to isolate binding proteins. One of the generated analogs inhibited insulin-stimulated glucose uptake with increased sensitivity compared with olomoucine. The IC50 for inhibition of insulin-stimulated glucose uptake occurred at analog concentrations as low as 0.1 μm. To identify proteins binding to the analog, [35S]-labeled cell lysates prepared from 3T3-L1 adipocytes were incubated with analog chemically cross-linked to a resin support and binding proteins analyzed by SDS-PAGE. The major binding species was a doublet at 50-60 kDa, which was identified as calcium/calmodulin-dependent protein kinase II (CaMKII) by N-terminal peptide analysis and confirmed by ...
Active calcium/calmodulin-dependent protein kinase II (CaMKII) has been reported to take a critical role in the induction of long-term potentiation (LTP). Changes in CaMKII activity were detected in various ischemia models. It is tempting to know whether and how CaMKII takes a role in NMDA receptor (NMDAR)-mediated postischemic long-term potentiation (NMDA i-LTP). Here, we monitored changes in NMDAR-mediated field excitatory postsynaptic potentials (NMDA fEPSPs) at different time points following ischemia onset |i|in vitro|/i| oxygen and glucose deprivation (OGD) ischemia model. We found that 10 min OGD treatment induced significant i-LTP in NMDA fEPSPs, whereas shorter (3 min) or longer (25 min) OGD treatment failed to induce prominent NMDA i-LTP. CaMKII activity or CaMKII autophosphorylation displays a similar bifurcated trend at different time points following onset of ischemia both |i|in vitro|/i| OGD or |i|in vivo|/i| photothrombotic lesion (PT) models, suggesting a correlation
TY - JOUR. T1 - Identification of a Ca2+/calmodulin-dependent protein kinase II regulatory phosphorylation site in non-N-methyl-D-aspartate glutamate receptors. AU - Yakel, Jerrel L.. AU - Vissavajjhala, Prabhakar. AU - Derkach, Victor A.. AU - Brickey, Debra A.. AU - Soderling, Thomas R.. PY - 1995/2/28. Y1 - 1995/2/28. N2 - Glutamate receptor ion channels are colocalized in postsynaptic densities with Ca2+/calmodulin-dependent protein kinase II (CaM-kinase II), which can phosphorylate and strongly enhance non-N-methyl-D-aspartate (NMDA) glutamate receptor current. In this study, CaM-kinase II enhanced kainate currents of expressed glutamate receptor 6 in 293 cells and of wild-type glutamate receptor 1, but not the Ser-627 to Ala mutant, in Xenopus oocytes. A synthetic peptide corresponding to residues 620-638 in GluR1 was phosphorylated in vitro by CaM-kinase II but not by cAMP-dependent protein kinase or protein kinase C. The 32P-labeled peptide map of this synthetic peptide appears to be the ...
TY - JOUR. T1 - Ca2+/calmodulin-dependent protein kinase II regulates Tiam1 by reversible protein phosphorylation. AU - Fleming, I N. AU - Elliott, C M. AU - Buchanan, F G. AU - Downes, C P. AU - Exton, J H. PY - 1999. Y1 - 1999. N2 - A number of guanine nucleotide exchange factors have been identified that activate Rho family GTPases, by promoting the binding of GTP to these proteins. We have recently demonstrated that lysophosphatidic acid and several other agonists stimulate phosphorylation of the Rac1-specific exchange factor Tiam1 in Swiss 3T3 fibroblasts, and that protein kinase C is involved in Tiam1 phosphorylation (Fleming, I. N., Elliott, C. M., Collard, J. G., and Exton, J. H. (1997) J. Biol. Chem. 272, 33105-33110). We now show, through manipulation of intracellular [Ca2+] and the use of protein kinase inhibitors, that both protein kinase Calpha and Ca2+/calmodulin-dependent protein kinase II are involved in the phosphorylation of Tiam1 in vivo. Furthermore, we show that ...
Results We show that culture of MRL/lpr Foxp3-GFP T cells in the presence of KN-93 promotes Treg differentiation in a dose dependent manner (Fig. F). Treatment of MRL/lpr Foxp3-GFP mice with KN-93 results in significant induction of Treg cells in the spleen, peripheral lymph nodes (Fig. B-E) and peripheral blood (Fig. A and B) and this is accompanied by decreased skin and kidney damage. Notably, KN-93 clearly diminishes the accumulation of inflammatory cells along with reciprocally increased Treg cells in target organ.. ...
TY - JOUR. T1 - Amphetamine activate protein kinase C and calcium/calmodulin-dependent protein kinase via NMDA receptor in primary cultures of rat cortical neurons. AU - Wu, Hsueh-Hsia. AU - Lee, Horng-Mo. PY - 1999. Y1 - 1999. M3 - Article. VL - 1. SP - 12. EP - 19. JO - New Taipei Journal of Medicine. JF - New Taipei Journal of Medicine. SN - 1562-4242. ER - ...
TY - JOUR. T1 - CaM Kinase II-dependent pathophysiological signalling in endothelial cells. AU - Cai, Hua. AU - Liu, Depei. AU - Garcia, Joe G.N.. N1 - Funding Information: The authors work are supported by National Heart, Lung, and Blood Institute (NHLBI) Grants HL-077440 (H.L.C.), HL-081571 (H.L.C.), HL057244 (Pin-Lan Li and H.L.C.), an American Heart Association Grant 0435189N (H.L.C.), an American Diabetes Association Award 7-04-RA-16 (H.L.C.), and NHLBI PPG HL 58064 (J.G.N.G.).. PY - 2008/1. Y1 - 2008/1. N2 - Calcium/calmodulin-dependent protein kinase II (CaM Kinase II) is a known modulator of cardiac pathophysiology. The present review uniquely focuses on novel CaM Kinase II-mediated endothelial cell signalling which, under pathophysiological conditions, may indirectly modulate cardiac functions via alterations in endothelial or endocardial responses. CaM Kinase II has four different isoforms and various splicing variants for each isoform. The endothelial cell CaM Kinase II isoforms are ...
Authors Affiliations: 1Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Istituto di Endocrinologia e Oncologia Sperimentale del CNR Naples; 2Department of Neuroscience, Reproductive and Odontostomatological Sciences, University of Naples Federico II, Naples; 3Department of Medicine-DIMED, Unit of Endocrinology, University of Padua, Padova; and 4Department of Medicine and Surgery, University of Salerno, Salerno, ...
There are comments on PubPeer for publication: Ca(2+)/calmodulin-dependent protein kinase II promotes cell cycle progression by directly activating MEK1 and subsequently modulating p27 phosphorylation (2009)
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Complete information for CAMK4 gene (Protein Coding), Calcium/Calmodulin Dependent Protein Kinase IV, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
The concept of subcellular targeting by anchoring proteins is of major importance for understanding the specificity of signal transduction. The work presented here constitutes the first description of an anchoring protein for multifunctional CaM kinase II. αKAP exhibits three properties expected of anchoring proteins. (i) It is restricted to a specific cellular compartment, it is membrane bound and probably directly inserted into SR membranes by its N‐terminal hydrophobic domain (Figures 3 and 4). (ii) It binds CaM kinase II. This binding occurs within intact cells and not during extraction of transfected cells, since significant interaction was only detected after coexpression of αKAP and CaM kinase II, but not when individually expressed proteins were mixed (Figure 6). (iii) It is responsible for the targeting of the novel βM‐CaM kinase II to the SR, since it co‐immunoprecipitates with kinase extracted from SR membranes and the kinase does not have the physical properties of a ...
Overview Protocols Specifications Resources Convenient, 1-Hour Kinase Activity Assays The SignaTECT® Protein Kinase Assay Systems provide a rapid, radioactivity-based method for detection of kinase activity. The method is based on capture of phosphorylated labeled substrates on the SAM2® Biotin Capture Membrane, which
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
TY - JOUR. T1 - β-Adrenergic induced SR Ca2 + leak is mediated by an Epac-NOS pathway. AU - Pereira, Laëtitia. AU - Bare, Dan J.. AU - Galice, Samuel. AU - Shannon, Thomas R.. AU - Bers, Donald M. PY - 2017/7/1. Y1 - 2017/7/1. N2 - Cardiac β-adrenergic receptors (β-AR) and Ca2 +-Calmodulin dependent protein kinase (CaMKII) regulate both physiological and pathophysiological Ca2 + signaling. Elevated diastolic Ca2 + leak from the sarcoplasmic reticulum (SR) contributes to contractile dysfunction in heart failure and to arrhythmogenesis. β-AR activation is known to increase SR Ca2 + leak via CaMKII-dependent phosphorylation of the ryanodine receptor. Two independent and reportedly parallel pathways have been implicated in this β-AR-CaMKII cascade, one involving exchange protein directly activated by cAMP (Epac2) and another involving nitric oxide synthase 1 (NOS1). Here we tested whether Epac and NOS function in a single series pathway to increase β-AR induced and CaMKII-dependent SR Ca2 + ...
Complete information for CAMKK2 gene (Protein Coding), Calcium/Calmodulin Dependent Protein Kinase Kinase 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
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The death-associated protein kinase (DAPK) family has been characterized as a group of pro-apoptotic serine/threonine kinases that share specific structural features in their catalytic kinase domain. Two of the DAPK family members, DAPK1 and DAPK2, are calmodulin-dependent protein kinases that are regulated by oligomerization, calmodulin binding, and autophosphorylation. In this study, we have determined the crystal and solution structures of murine DAPK2 in the presence of the autoinhibitory domain, with and without bound nucleotides in the active site. The crystal structure shows dimers of DAPK2 in a conformation that is not permissible for protein substrate binding. Two different conformations were seen in the active site upon the introduction of nucleotide ligands. The monomeric and dimeric forms of DAPK2 were further analyzed for solution structure, and the results indicate that the dimers of DAPK2 are indeed formed through the association of two apposed catalytic domains, as seen in the ...
calmodulin-dependent protein kinase V: widely distributed in various tissues, involved in calcium-regulated processes; from rat brain; may exist in 40 & 41 kDa isoforms; amino acid sequence has been determined
Nuclear factor-kappa beta (NF-kβ) pro-inflammatory signalling is important in modulating endothelial dysfunction and may be important in vascular dysfunction associated with the ageing process. Recent studies in the heart have highlighted Calcium/calmodulin-dependent protein kinase II (CaMKII) as a novel regulator of NF-kβ signalling. However nothing is known of the role this interaction could play in regulating dysfunction of the vasculature during ageing. Here we (i) characterise NF-kβ signalling in vascular endothelial cells and examine the potential for CaMKII modulation and (ii) determine whether CaMKIIδ expression is altered in ageing.. Using human umbilical vein endothelial cells (HUVECs) as an in vitro model system, initial experiments have established that pro-inflammatory NF-kB signalling is activated in response to both tumour necrosis factor α (TNF-α) and interleukin-1β (IL-1β) stimulation. This was shown by a significant reduction in IkBα expression (1.18 ± 0.16 vs. 0.48 ...
p42/microtubule-associated protein kinase (p42mapk) is activated by tyrosine and threonine phosphorylation, and its regulatory phosphorylation is likely to be important in signalling pathways involved in growth control, secretion, and differentiation. Here we show that treatment of quiescent 3T3 cells with diverse agonists results in the appearance of an activity capable of causing the in vitro phosphorylation of p42mapk on the regulatory tyrosine and to a lesser extent on the regulatory threonine, resulting in enzymatic activation of the p42mapk. This p42mapk-activating activity is capable of phosphorylating a kinase-defective p42mapk mutant, thus confirming its activity as a kinase. ...
CaMKII alpha antibody (calcium/calmodulin-dependent protein kinase II alpha) for ICC/IF, IHC-Fr, WB. Anti-CaMKII alpha pAb (GTX127939) is tested in Mouse, Rat samples. 100% Ab-Assurance.
Wet-lab validated real-time PCR primer assays for your biological pathway of interest. Select your gene target of interest using an interactive pathway map, and select your plate.
Camk1g - Camk1g (untagged ORF) - Rat calcium/calmodulin-dependent protein kinase IG (Camk1g), (10 ug) available for purchase from OriGene - Your Gene Company.
Camk2d2 antibody (calcium/calmodulin-dependent protein kinase (CaM kinase) II delta 2) for IHC-P, WB. Anti-Camk2d2 pAb (GTX124377) is tested in Zebrafish samples. 100% Ab-Assurance.
Full-length recombinant human CAMK2δ was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CAMK2δ is a serine/threonine protein kinase that is a member of the type II multifunctional Ca2+/calmodulin-dependent protein kinase family.
FUNCTION: ACTIVATES TYROSINE AND TRYPTOPHAN HYDROXYLASES IN THE PRESENCE OF CA(2+)/CALMODULIN-DEPENDENT PROTEIN KINASE II, AND STRONGLY ACTIVATES PROTEIN KINASE C. IS PROBABLY A MULTIFUNCTIONAL REGULATOR OF THE CELL SIGNALING PROCESSES MEDIATED BY BOTH KINASES ...
Gentaur molecular products has all kinds of products like :search , FabGennix \ Calmdulin kinase gamma, Host species: Rabbit, Polyclonal antibody \ CaMK-321AP for more molecular products just contact us
This study investigates the roles of hippocampal N-methyl-D-aspartate (NMDA) glutamate receptors and CaMKII (calcium/calmodulin-dependent protein kinase II) in amphetamine-produced conditioned place preference (AMPH-CPP) in rats. An earlier report showed that AMPH-CPP resulted in the enhancement of …
Abstract. Serine/threonine kinases (STKs) represent the majority of discovered kinases to date even though a few Food and Drug Administration approved STKs inhibitors are reported. The third millennium came with the discovery of an important group of STKs that reshaped our understanding of several biological signaling pathways. This family was named death-associated protein kinase family (DAPK family). DAPKs comprise five members (DAPK1, DAPK2, DAPK3, DRAK1, and DRAK2) and belong to the calcium/calmodulin-dependent kinases domain. As time goes on, the list of biological functions of this family is constantly updated. The most extensively studied member is DAPK1 (based on the publications number and Protein Data Bank reported crystal structures) that plays fundamental biological roles depending on the cellular context. DAPK1 regulates apoptosis, autophagy, contributes to the pathogenesis of Alzheimers disease, acts as a tumor suppressor, inhibits metastasis, mediates the body responses to viral ...
Death-associated protein kinase (DAPK) is a pro-apoptotic serine/threonine kinase involved in apoptosis. Aberrant methylation of DAPK was reported in lung cancers by methylation-specific PCR. However, we were unable to relate methylation with gene silencing with the same methodology. Our goals were to develop a methodology that related methylation with gene silencing and use it to study the state of the gene in lung cancers.. Using a semiquantitative real-time reverse transcription-PCR, DAPK expression was lower in lung cancers than in corresponding nonmalignant bronchial epithelial cells in five of six primary short-term cultures. In continuous cell lines, mRNA expression was down-regulated, as well as compared with nonmalignant bronchial epithelial cells, and its protein was not detected by Western blotting in 17 of 23 (74%) cell lines. We investigated methylation status of 5 flanking region of DAPK by combined bisulfite restriction analysis and bisulfited DNA sequencing. Aberrant methylation ...
Interleukin-1β (IL-1β) is critical for inflammation and control of infection. The production of IL-1β depends on expression of pro-IL-1β and inflammasome component induced by inflammatory stimuli, followed by assembly of inflammasome to generate caspase-1 for cleavage of pro-IL-1β. Here we show that tumor suppressor death-associated protein kinase (DAPK) deficiency impaired IL-1β production in macrophages. Generation of tumor necrosis factor-α in macrophages, in contrast, was not affected by DAPK knockout. Two tiers of defects in IL-1β generation were found in DAPK-deficient macrophages: decreased pro-IL-1β induction by some stimuli and reduced caspase-1 activation by all inflammatory stimuli examined. With a normal NLRP3 induction in DAPK-deficient macrophages, the diminished caspase-1 generation is attributed to impaired inflammasome assembly. There is a direct binding of DAPK to NLRP3, suggesting an involvement of DAPK in inflammasome formation. We further illustrated that the formation of
Raval A, Tanner SM, Byrd JC, Angerman EB, Perko JD, Chen SS, Hackanson B, Grever MR, Lucas DM, Matkovic JJ, Lin TS, Kipps TJ, Murray F, Weisenburger D, Sanger W, Lynch J, Watson P, Jansen M, Yoshinaga Y, Rosenquist R, de Jong PJ, Coggill P, Beck S, Lynch H, de la Chapelle A, Plass C. Downregulation of death-associated protein kinase 1 (DAPK1) in chronic lymphocytic leukemia. Cell. 2007 Jun 01; 129(5):879-90 ...
Three genes encoding different Ca2+/calmodulin-dependent protein kinases have been characterized in the wheat phytopathogenic fungus Stagonospora nodorum. The kinases were identified from the S. nodorum genome sequence on the basis of sequence homology to known Ca2+/calmodulin- dependent protein kinases. Expression analysis determined that each of the kinases was expressed during growth in vitro and also during infection. The onset of sporulation triggered increased transcript levels of each of the kinases, particularly CpkA where an 11-fold increase in expression was observed during sporulation in planta. The role of the kinases was further determined via a reverse genetics approach. The disruption of CpkA affected vegetative growth in vitro and also sporulation. The cpkA strains produced 20-fold less spores on complex media and were unable to sporulate on defined minimal media. Infection assays showed that CpkA was not required for lesion development but was essential for sporulation at the ...
Excessive activation of β-adrenergic, angiotensin II, and aldosterone signaling pathways promotes mortality after myocardial infarction (MI), while antagonist drugs targeting these pathways are core therapies for treating post-MI patients. The multifunctional calcium/calmodulin-dependent protein kinase II (CaMKII) is activated by catecholamines and angiotensin II, and CaMKII inhibition prevents isoproterenol- and angiotensin II-mediated cardiomyopathy. Here we ask the hypothesis if aldosterone and CaMKII participated in common responses to MI by developing a mouse MI model supplemented by aldosterone infusion (MI+Aldo) to approximate plasma aldosterone levels measured in MI patients. We find that aldosterone exerts direct toxic actions on myocardium by oxidative activation of CaMKII, causing cardiac rupture and increased mortality in mice after MI (65.5% for aldosterone versus 31.0% for vehicle, P=0.007, n≥19 mice per treatment). Aldosterone oxidizes CaMKII by recruiting NADPH oxidase, and ...
article{dd19855c-025e-4a5e-9215-43f4f9e9a4a1, abstract = {The NMDA receptor complex represents a key molecular element in the pathogenesis of long-term synaptic changes and motor abnormalities in Parkinsons disease (PD). Here we show that NMDA receptor 1 (NR1) subunit and postsynaptic density (PSD)-95 protein levels are selectively reduced in the PSD of dopamine (DA)-denervated striata. These effects are accompanied by an increase in striatal levels of alphaCa(2+)-calmodulin-dependent protein kinase II (alphaCaMKII) autophosphorylation, along with a higher recruitment of activated alphaCaMKII to the regulatory NMDA receptor NR2A-NR2B subunits. Acute treatment of striatal slices with R(+)-7-chloro-8-hydroxy-3-methyl-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzaz epine hydrochloride, but not with L-sulpiride, mimicked the effect of DA denervation on both alphaCaMKII autophosphorylation and corticostriatal synaptic plasticity. In addition to normalizing alphaCaMKII autophosphorylation levels as well as ...
Looking for online definition of CaM kinase II delta subunit in the Medical Dictionary? CaM kinase II delta subunit explanation free. What is CaM kinase II delta subunit? Meaning of CaM kinase II delta subunit medical term. What does CaM kinase II delta subunit mean?
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Stress-activated protein kinases (SAPKs) are stimulated by cell damaging agents as well as by physiological receptor agonists. In this study we show that human platelets contain the isoforms SAPK2a, SAPK2b, SAPK3 and SAPK4 as determined by immunoblotting with specific antibodies. All four kinases were activated in thrombin-stimulated platelets whereas only SAPK2a and SAPK2b were significantly stimulated by collagen. All four isoforms were able to phosphorylate wild-type human cPLA2in vitro, although to different extents, but not cPLA2 mutants that had Ser505 replaced by alanine. Phosphorylation at Ser505 was confirmed by phosphopeptide mapping using microbore HPLC. SAPK2a and 42-kDa mitogen-activated protein kinase incorporated similar levels of phosphate into cPLA2 relative to the ability of each kinase to stimulate phosphorylation of myelin basic protein. SAPK2b and SAPK4 incorporated less phosphate, and cPLA2 was a poor substrate for SAPK3. The inhibitor of SAPK2a and SAPK2b, SB 202190, ...
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InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
We investigated the effects of Wenxin Keli (WXKL) on the Calcium/Calmodulin dependent kinase II (CaMK II) signal transduction pathway with transverse aortic constriction (TAC) rats. Echocardiographic measurements were obtained 3 and 9 weeks after the surgery. Meanwhile, the action potentials (APDs) were recorded using the whole-cell patch clamp technique, and western blotting was used to assess components of the CaMK II signal transduction pathway. At both 3 and 9 weeks after treatment, the fractional shortening (FS%) increased in the WXKL group compared with the TAC group. The APD|sub|90|/sub| of the TAC group was longer than that of the Sham group and was markedly shortened by WXKL treatment. Western blotting results showed that the protein expressions of CaMK II, phospholamban (PLB), and ryanodine receptor 2 (RYR2) were not statistically significant among the different groups at both treatment time points. However, WXKL treatment decreased the protein level and phosphorylation of CaMK II (Thr
Background: Alzheimers Disease (AD) is a neuron related brain disorder leading to reasoning and memory loss. There is no specific cure identified for AD. JNK3 (c-Jun N-terminal kinase /stress-activated protein kinase) are highly revealed within the central nervous system, particularly neurons, playing vital role in functioning of brain. JNK3 hyper phosphorylation is a very common conclusion in neurodegenerative diseases. JNK3 in turn hyper phosphorylates Amyloid Precursor Protein (APP) which leads to the formation of Amyloid β peptides (an inductive agent of Alzheimers disease). Methods: Protein JNK-3 (PDB ID: 3KVX) was retrieved from protein data bank and later we docked a library of compounds against it. These were further validated by ADMET studies. Results: Thus, docking inhibitors of JNK3 may provide a promising sanitive approach. Based on best docking score and glide score a potential lead is identified against JNK3. Conclusion: Inhibiting JNK-3 may lead to less production of amyloidβ ...
immune Uncategorized Dinaciclib (SCH 727965) manufacture, Rabbit polyclonal to NFKBIZ. Activation from the RNA-dependent proteins kinase (PKR) continues to be implicated in the pathogenesis of several neurodegenerative illnesses. not really mediated by PKR inhibition. Using kinase assays we looked into whether PKRi impacts any other proteins kinase. These analyses proven that PKRi does not Dinaciclib (SCH 727965) manufacture have any major inhibitory influence on pro-apoptotic kinases like the c-Jun N-terminal kinases (JNKs), the p38 MAP kinases as well as the death-associated proteins kinases (DAPKs), or on additional kinases including c-Raf, MEK1, MKK7 and MKK6. PKRi does, nevertheless, inhibit the experience of particular cyclin-dependent kinases (CDKs) including CDK2 and CDK5 both and in LK-treated neurons. In keeping with its inhibitory actions on mitotic CDKs, the treating HT-22 and HEK293T cell lines with PKRi decreases the pace of cell cycle progression sharply. Taken alongside the ...
Anti-peptide antibodies specific for the neuronal calcium channel alpha 1E subunit (anti-CNE1 and anti-CNE2) were produced to study the biochemical properties and subcellular distribution of the alpha 1E polypeptide from rat brain. Immunoblotting identified a single size form of 245-255 kDa which was a substrate for phosphorylation by cAMP- dependent protein kinase, protein kinase C, cGMP-dependent protein kinase, and calcium/calmodulin-dependent protein kinase II. Ligand- binding studies of alpha 1E indicate that it is not a high affinity receptor for the dihydropyridine isradipine or the peptide toxins omega- conotoxin GVIA or omega-conotoxin MVIIC at concentrations which elicit high affinity binding to other channel types in the same membrane preparation. The alpha 1E subunit is widely distributed in the brain with the most prominent immunocytochemical staining in deep midline structures such as caudate-putamen, thalamus, hypothalamus, amygdala, cerebellum, and a variety of nuclei in the ...
CAMK2A - CAMK2A (Myc-DDK-tagged)-Human calcium/calmodulin-dependent protein kinase II alpha (CAMK2A), transcript variant 2 available for purchase from OriGene - Your Gene Company.
Since its inception, the synaptic tagging hypothesis has inspired many to search for synaptic tags. However, very few molecules have been proposed as candidates (Frey and Frey, 2008). The nature and identity of PRPs and synaptic tags are under intensive investigations (Frey and Frey, 2008). Two pathway experiments showed that blockade of protein kinase A (PKA) or its interaction with A kinase-anchoring proteins (AKAPs) prevents synaptic capture, suggesting that PKA or its anchoring at active synapses may serve as a synaptic tag for L-LTP (Huang et al., 2006; Young et al., 2006). Calcium/calmodulin-dependent protein kinase II (CaMKII) is also implicated as an L-LTP-specific tag (Sajikumar et al., 2007). Studies thus far have been based on the use of pharmacological inhibitors in two-pathway experiments. However, it is necessary to show that these tags are transiently and locally activated in a protein synthesis-independent manner by weak stimulation. In a recent study, NMDA-dependent, ...
Multiple calmodulin (CaM) isoforms are expressed in plants, but their biochemical characteristics are not well resolved. Here we show the differential regulation exhibited by two soya bean CaM isoforms (SCaM-1 and SCaM-4) for the activation of five CaM-dependent enzymes, and the Ca2+ dependence of their target enzyme activation. SCaM-1 activated myosin light-chain kinase as effectively as brain CaM (Kact 1.8 and 1.7nM respectively), but SCaM-4 produced no activation of this enzyme. Both CaM isoforms supported near maximal activation of CaM-dependent protein kinase II (CaM KII), but SCaM-4 exhibited approx.12-fold higher Kact than SCaM-1 for CaM KII phosphorylation of caldesmon. The SCaM isoforms showed differential activation of plant and animal Ca2+-ATPases. The plant Ca2+-ATPase was activated maximally by both isoforms, while the erythrocyte Ca2+-ATPase was activated only by SCaM-1. Plant glutamate decarboxylase was activated fully by SCaM-1, but SCaM-4 exhibited an approx. 4-fold increase ...
DAPK1 / DAP Kinase Protein LS-G97367 is a Recombinant Human DAPK1 / DAP Kinase produced in Baculovirus Met 1-Leu 363 with His-GST tag(s). It is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method).
Supplementary MaterialsAdditional document 1: Body S1. for profiling little RNAs. Body S15. The saturation curves of miRNA. Body S16. RPM scatterplots of portrayed small RNAs. Body S17. Comparative expression heat maps of super-enhancer-regulated professional mRNAs and miRNAs. Body S18. Hematoxylin and Eosin (HE) staining from the HCC tissues. Figure S19. Comparative expression degrees of gene groupings between HCC Exp-subpopulations. Body S20. mRNA catch sequencing from the Holo-Seq total RNA collection. Body S21. mRNA and miRNA single transcriptome analyses of hepatocellular carcinoma (HCC) one cells. (DOCX 5908 kb) 13059_2018_1553_MOESM1_ESM.docx (5.7M) GUID:?8BF5D1B7-5F74-410D-8E95-CCE7DDE5D5D7 Extra file YM348 2: Desk S1. Not really1-site-containing transcripts in mouse. Desk S2. Not really1-site-containing transcripts in individual. Desk S3. Sequencing figures of RNA libraries. Desk S4. One cell collection price with different strategies. (XLSX 171 kb) 13059_2018_1553_MOESM2_ESM.xlsx ...
The Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) is critically required for the synaptic recruitment of AMPA-type glutamate receptors (AMPARs) during both development and plasticity. However, the underlying mechanism is unknown. Using single-particle tracking of AMPARs, we show that CaMKII …
1MXE: Structure of the Complex of Calmodulin with the Target Sequence of Calmodulin-Dependent Protein Kinase I: Studies of the Kinase Activation Mechanism
KN-92 is an inactive derivative of KN-93. KN-93 is a selective inhibitor of Ca2+/calmodulin-dependent kinase II (CaMKII), competitively blocking CaM binding to the kinase (Ki = 370 nM).
Heart failure stays a significant well being burden around the globe. Despite nice progress in delineation of molecular mechanisms underlying growth of illness, normal remedy has not superior on the similar tempo. The multifunctional signaling molecule Ca2+/calmodulin-dependent protein kinase II (CaMKII) has acquired appreciable consideration over current years for its central function in maladaptive reworking and arrhythmias … Read more. ...
Some receptors for growth factors activate a protein kinase cascade, with the participation of multiple enzymes to effect a change in gene expression. Which of the following statements about a protein kinase cascade are true?. ...
Click on a [studies] link to search within your current results for studies in that region. Use the back button to return to this list and try another region ...
Click on a [studies] link to search within your current results for studies in that region. Use the back button to return to this list and try another region ...
Calcium/calmodulin dependent protein kinase IG is a protein that in humans is encoded by the CAMK1G gene. This gene encodes a ... "Entrez Gene: Calcium/calmodulin dependent protein kinase IG". Retrieved 2018-08-28. Takemoto-Kimura S, Terai H, Takamoto M, ... protein similar to calcium/calmodulin dependent protein kinase, however, its exact function is not known. [provided by RefSeq, ... a novel membrane-anchored neuronal Ca2+/calmodulin-dependent protein kinase (CaMK)". J. Biol. Chem. 278 (20): 18597-605. doi: ...
Calcium/calmodulin-dependent protein kinases. References[edit]. .mw-parser-output .reflist{font-size:90%;margin-bottom:0.5em; ... which contain residues that can be directly modified by a series of protein kinases and protein phosphatases, as well as ... "MHC class I immune proteins are critical for hippocampus-dependent memory and gate NMDAR-dependent hippocampal long-term ... Yu XM, Askalan R, Keil GJ, Salter MW (January 1997). "NMDA channel regulation by channel-associated protein tyrosine kinase Src ...
Picciotto, M. R.; Czernik, A. J.; Nairn, A. C. (15 December 1993). "Calcium/calmodulin-dependent protein kinase I. cDNA cloning ... "Calcium/calmodulin-dependent protein kinase I - cDNA cloning and identification of autophosphorylation site". J. Biol. Chem. ... work with Paul Greengard at Rockefeller University where she cloned the gene for calcium/calmodulin-dependent protein kinase 1 ... "Menthol disrupts nicotine's psychostimulant properties in an age and sex-dependent manner in C57BL/6J mice". Behav. Brain Res. ...
... calcium/calmodulin-dependent protein kinase (CaM kinase) II delta". Hook SS, Means AR (2001). "Ca2+/CaM-dependent ... Calcium/calmodulin-dependent protein kinase type II delta chain is an enzyme that in humans is encoded by the CAMK2D gene. The ... product of this gene belongs to the serine/threonine protein kinase family and to the Ca2+/calmodulin-dependent protein kinase ... "Rad and Rad-related GTPases interact with calmodulin and calmodulin-dependent protein kinase II". J. Biol. Chem. 272 (18): ...
... is phosphorylated by the calcium-calmodulin-dependent protein kinase. MAPKAPK2 (mitogen-activated-protein kinase-activating ... that are phosphorylated by a variety of protein kinases. Ser40 is phosphorylated by the cAMP-dependent protein kinase. Ser19 ( ... "Molecular cloning of cDNA coding for brain-specific 14-3-3 protein, a protein kinase-dependent activator of tyrosine and ... Tyrosine hydroxylase is activated by phosphorylation dependent binding to 14-3-3 proteins. Since the 14-3-3 proteins also are ...
Activation of calcium/calmodulin-dependent protein kinase II in obesity mediates suppression of hepatic insulin signaling". ... and a calcium-induced apoptosis pathway, which involves an ER calcium-release channel IP3R, a calcium-sensitive protein kinase ... Tabas discovered a calcium-IP3R-CaMKII pathway plays a key role in glucagon-mediated excessive hepatic glucose production, ... One notable finding showed a critical link between the PERK / CHOP branch of the stress Unfolded protein response (UPR) ...
This sudden increase in cytoplasmic calcium activates Ca2+/calmodulin-dependent protein kinase (CaMKII). Activated CaMKII ... Ren J, Li Y, Kufe D (May 2002). "Protein kinase C delta regulates function of the DF3/MUC1 carcinoma antigen in beta-catenin ... There is one additional requirement: Similar to the mitogen-activated protein kinases (MAPKs), substrates need to associate ... Thus it also requires a "priming kinase" for its activities. In the case of beta-catenin, the most important priming kinase is ...
"Calcium/calmodulin-dependent protein kinase II phosphorylation drives synapse-associated protein 97 into spines". The Journal ... is the influx of calcium through the NMDA receptors and the resultant activation of Ca2+/calmodulin-dependent protein kinase ( ... element-binding protein through a phosphatidylinositol 3-kinase-dependent stimulation of the mitogen-activated protein kinase ... "Long-term potentiation is associated with an increased activity of Ca2+/calmodulin-dependent protein kinase II". The Journal of ...
"Liprinalpha1 degradation by calcium/calmodulin-dependent protein kinase II regulates LAR receptor tyrosine phosphatase ... The protein encoded by this gene is a member of the LAR protein tyrosine phosphatase-interacting protein (liprin) family. ... Protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1 has been shown to ... "Entrez Gene: PPFIA1 protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1 ...
This gene is also known by several other names: CMG 2 (CAMGUK protein 2), calcium/calmodulin-dependent serine protein kinase 3 ... "Entrez Gene: CASK Calcium/calmodulin-dependent serine protein kinase (MAGUK family)". Tarpey PS, Smith R, Pleasance E, Whibley ... Zhu ZQ, Wang D, Xiang D, Yuan YX, Wang Y (2014). "Calcium/calmodulin-dependent serine protein kinase is involved in exendin-4- ... "Association of junctional adhesion molecule with calcium/calmodulin-dependent serine protein kinase (CASK/LIN-2) in human ...
Ng J, Rashid AJ, So CH, O'Dowd BF, George SR (January 2010). "Activation of calcium/calmodulin-dependent protein kinase IIalpha ... dual functional regulation by G protein-coupled receptor kinase 2". The Journal of Biological Chemistry. 285 (45): 35092-103. ... Hasbi A, Fan T, Alijaniaram M, Nguyen T, Perreault ML, O'Dowd BF, George SR (December 2009). "Calcium signaling cascade links ... In comparison, signalling of the homologous D5-D2 receptor heteromer involves the influx of extracellular calcium. The D1-D2 ...
... resulting in a calcium influx into the SCN. Calcium induces the activity of Ca2+/calmodulin-dependent protein kinases, ... and Ca2+/calmodulin-dependent protein kinases on the Serine 133 residue. When activated, CREB protein recruits other ... This protein kinase translocates to the cell nucleus, where it activates a CREB protein. The activated CREB protein then binds ... Abnormalities of a protein that interacts with the KID domain of CREB, the CREB-binding protein, (CBP) is associated with ...
Like neuroligins, neurexins possess a PDZ-domain that associates with CASK (Calcium-calmodulin-dependent protein kinase). In ... Cadherins are calcium- dependent, homophilic cell adhesion molecules that form complexes with cytosolic partners known as ... This interaction, mediated by ephrin A3/EphA4 signaling, induces the recruitment and activation of cyclin-dependent kinase 5 ( ... Components of this complex bind to a number of different scaffolding proteins, phosphotases, kinases, and receptors. Classical ...
"Entrez Gene: CAMK2B calcium/calmodulin-dependent protein kinase (CaM kinase) II beta". Walikonis RS, Oguni A, Khorosheva EM, ... Calcium/calmodulin-dependent protein kinase type II beta chain is an enzyme that in humans is encoded by the CAMK2B gene. The ... Sequence analyses of human brain calcium/calmodulin-dependent protein kinase II". Mol. Biol. Rep. 28 (1): 35-41. doi:10.1023/A: ... Novak G, Seeman P, Tallerico T (2001). "Schizophrenia: elevated mRNA for calcium-calmodulin-dependent protein kinase IIbeta in ...
Bouvard D, Block MR (1998). "Calcium/calmodulin-dependent protein kinase II controls integrin alpha5beta1-mediated cell ... 2007). "MAP kinase-dependent, NF-kappaB-independent regulation of inhibitor of apoptosis protein genes by TNF-alpha". J. Cell. ... 2006). "Integrin cytoplasmic domain-associated protein-1 (ICAP-1) interacts with the ROCK-I kinase at the plasma membrane". J. ... The protein encoded by this gene binds to the beta1 integrin cytoplasmic domain. The interaction between this protein and beta1 ...
In an experiment using virus-induced gene silencing of two calcium-dependent protein kinases (CDPKs) in a wild tobacco ( ... are turned on by Ca2+ dependent protein kinases. In Arabidopsis, over expression of the IQD1 calmodulin-binding transcriptional ... This increase in cytosolic concentration activates target proteins such as Calmodulin and other binding proteins. Downstream ... When fatty acid amides are present in insect saliva, the mitogen-activated protein kinases (MAPKs) are activated. These genes ...
"Association of junctional adhesion molecule with calcium/calmodulin-dependent serine protein kinase (CASK/LIN-2) in human ... "Association of junctional adhesion molecule with calcium/calmodulin-dependent serine protein kinase (CASK/LIN-2) in human ... "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome ... KDR+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH) This article incorporates text from ...
1999). "Death-associated protein kinase 2 is a new calcium/calmodulin-dependent protein kinase that signals apoptosis through ... Membrane protein MLC1 is a protein that in humans is encoded by the MLC1 gene. MLC1 (also called WKL1) is the only human gene ... homology to other proteins suggests that it may be an integral membrane transport protein. Mutations in this gene have been ... The MLC1 protein contains six putative transmembrane domains (S1-S6) and a pore region (P) between S5 and S6. Furthermore, MLC1 ...
... for nuclear calcium oscillations and a nuclear localized complex comprising a calcium-and-calmodulin dependent protein kinase ... and calmodulin-dependent protein kinase CCaMK. Moreover, the role of CYCLOPS, initially annotated as a protein with unknown ... Deregulation of a Ca2+/calmodulin-dependent kinase leads to spontaneous nodule development. Nature Volume: 441 Issue: 7097 ... The Parniske lab discovered that CYCLOPS is an interactor and phosphorylation substrate of the calcium- ...
... a protein kinase mediating biological effects downstream of Rho GTPases. Calcium/calmodulin-dependent protein kinase II gamma ( ... Harvey BP, Banga SS, Ozer HL (2004). "Regulation of the multifunctional Ca2+/calmodulin-dependent protein kinase II by the PP2C ... are Ca2+/calmodulin-dependent protein kinase phosphatases that promote apoptosis". J Biol Chem. 276 (47): 44193-202. doi: ... Protein phosphatase 1F is an enzyme that in humans is encoded by the PPM1F gene. The protein encoded by this gene is a member ...
These include calcium-dependent calmodulin, CaMKII (calmodulin-dependent protein kinase II), PKC (Protein Kinase C), PKA ( ... Calcium influx into the cell through NMDA receptors binds to calmodulin and activates the Ca2+/calmodulin-dependent protein ... Protein Kinase A), Protein Phosphatase-1 (PP-1), and Fyn tyrosine kinase. Certain signallers, such as CaMKII, are upregulated ... The activation of the RhoA protein will activate ROCK, a RhoA kinase, which leads to the stimulation of LIM kinase, which in ...
Ca2+/calmodulin-dependent protein kinase II-protein phosphatase 1 switch facilitates specificity in postsynaptic calcium ... calmodulin-dependent protein kinase II (CaMKII), which leads to LTP. The difference in Ca2+ concentration required for a cell ... in the phosphorylation of isocitrate dehydrogenase and in the activation of the calmodulin-dependent protein kinase II (CAMKII ... called MAPK kinase, or MAPKK. Similarly, MAPKK is activated by MAPKK kinase, or MAPKKK. These kinases are sequentially ...
"CAMK2A calcium/calmodulin dependent protein kinase II alpha [Homo sapiens (human)] - Gene - NCBI". www.ncbi.nlm.nih.gov. ... β CAMKIIδ CAMKIIγ CAMKIII CAMKIV CAMKV CaM kinase like vesicle associated SCAMK Ca2+/calmodulin-dependent protein kinase kinase ... CAMK, also written as CaMK, is an abbreviation for the Ca2+/calmodulin-dependent protein kinase class of enzymes. CAMKs are ... Hudmon A, Schulman H (2002-06-01). "Neuronal CA2+/calmodulin-dependent protein kinase II: the role of structure and ...
The increase in presynaptic calcium concentration activates calcium-calmodulin-dependent protein kinase (CaMK). CaMK ... Voltage-dependent calcium channel (VDCC) Allows the rapid influx of calcium during an action potential. ... The protein ELKS binds to the cell adhesion protein, β-neurexin, and other proteins within the complex such as Piccolo and ... that cause a local influx of calcium. The increase in calcium is detected by proteins in the active zone and forces vesicles ...
2005). "Multivalent interactions of calcium/calmodulin-dependent protein kinase II with the postsynaptic density proteins NR2B ... 2001). "Densin-180 forms a ternary complex with the (alpha)-subunit of Ca2+/calmodulin-dependent protein kinase II and (alpha)- ... portray Densin-180 as a key interactor in the midst of receptor proteins, scaffolding proteins and structural proteins. [number ... 2007). "Large-scale mapping of human protein-protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi: ...
Ca2+ quickly enters and binds to calmodulin. Calmodulin activates calcium/calmodulin-dependent protein kinase II (CaMKII), ... and calcium ions. Calcium ion entry into the presynaptic terminal causes the presynaptic release of glutamate, which diffuses ... This gives the NMDAR the property of being voltage-dependent, in that it requires strong postsynaptic depolarization to allow ...
Class IIa HDACs are phosphorylated by calcium/calmodulin dependent-kindase (CaMK) and protein kinase D (PKD) in response to ... This is most likely to allow for substrate recognition and protein-protein interactions that are necessary for class IIa HDAC ... This overexpression promoted lipopolysaccharide (LPS)-inducible expression of HDAC-dependent genes via a HIF-1alpha-dependent ... The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous ...
... calmodulin-dependent protein kinase (CAMK) subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) ... calmodulin-dependent protein kinase I by calmodulin and by Ca2+/calmodulin-dependent protein kinase kinase". J. Biol. Chem. 273 ... calmodulin-dependent protein kinase IV by Ca(2+)-calmodulin-dependent protein kinase Ia kinase. Phosphorylation of threonine ... calmodulin-dependent protein kinase kinase alpha by cAMP-dependent protein kinase: I. Biochemical analysis". J. Biochem. 130 (4 ...
Phosphorylation by cyclic AMP-dependent protein kinase, protein kinase C, and calcium/calmodulin protein kinase; identification ... calmodulin-dependent protein kinase I by calmodulin and by Ca2+/calmodulin-dependent protein kinase kinase". J. Biol. Chem. 273 ... Calcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein ... Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly ...
Calcium channel, voltage-dependent, L type, alpha 1C subunit (also known as Cav1.2) is a protein that in humans is encoded by ... When depolarization occurs, calcium ions flow through the channel and some bind to Calmodulin. The Calcium/Calmodulin binding ... Perets T, Blumenstein Y, Shistik E, Lotan I, Dascal N (Apr 1996). "A potential site of functional modulation by protein kinase ... This gene encodes an alpha-1 subunit of a voltage-dependent calcium channel. Calcium channels mediate the influx of calcium ...
... p39 interact with the alpha-subunit of Ca2+/calmodulin-dependent protein kinase II and alpha-actinin-1 in a calcium-dependent ... p39 interact with the alpha-subunit of Ca2+/calmodulin-dependent protein kinase II and alpha-actinin-1 in a calcium-dependent ... positive regulation of protein kinase activity. • regulation of cyclin-dependent protein serine/threonine kinase activity. • ... cyclin-dependent protein kinase 5 activator activity. • lipid binding. Cellular component. • cytoplasm. • cyclin-dependent ...
positive regulation of non-membrane spanning protein tyrosine kinase activity. • transmembrane receptor protein tyrosine kinase ... TrkB autophosphorylation is dependent upon its ligand-specific association with BDNF, a widely expressed activity-dependent ... triggering a calcium influx. Through a protein signaling cascade requiring Erk, CaM KII/IV, PI3K, and PLC, NMDA receptor ... Zhong L, Yan CH, Lu CQ, Xu J, Huang H, Shen XM (September 2009). "Calmodulin activation is required for the enhancement of ...
see MAP kinase pathway. Calcium. *Intracellular calcium-sensing proteins. *Calcineurin. *Ca2+/calmodulin-dependent protein ... GTP-binding protein regulators regulate G proteins in several different ways. Small GTPases act as molecular switches in ... and thus requires another class of regulatory proteins to accelerate this activity, the GTPase activating proteins (GAPs). ... GTP-Binding+Protein+Regulators at the US National Library of Medicine Medical Subject Headings (MeSH) ...
S6 kinase, protein kinase A (PKA), protein kinase C, Cdc2, and/or a Ca2+/calmodulin-dependent protein kinase; b) moves to bind ... Hammarberg T, Reddy KV, Persson B, Rådmark O (2002). "Calcium binding to 5-lipoxygenase". Adv. Exp. Med. Biol. Advances in ... ALOX5 binds with the F actin-binding protein, coactin-like protein. Based on in vitro studies, this protein binding serves to ... Upon cell stimulation, ALOX5: a) may be phosphorylated on serine 663, 523, and/or 271 by Mitogen-activated protein kinases, ...
"Calcium/calmodulin-dependent protein kinase II phosphorylation drives synapse-associated protein 97 into spines". J. Biol. Chem ... is the influx of calcium through the NMDA receptors and the resultant activation of Ca2+/calmodulin-dependent protein kinase ( ... "Long-term potentiation is associated with an increased activity of Ca2+/calmodulin-dependent protein kinase II". J. Biol. Chem ... element-binding protein through a phosphatidylinositol 3-kinase-dependent stimulation of the mitogen-activated protein kinase ...
Calcium-binding proteins. Intracellular calcium-sensing proteins. *Calmodulin. *Calnexin. *Calreticulin. *Gelsolin. *neuronal * ... Cala SE, Jones LR (January 1991). "Phosphorylation of cardiac and skeletal muscle calsequestrin isoforms by casein kinase II. ... Calsequestrin is a calcium-binding protein of the sarcoplasmic reticulum. The protein helps hold calcium in the cisterna of the ... The release of calsequestrin-bound calcium (through a calcium release channel) triggers muscle contraction. The active protein ...
The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats, a ... "The tyrosine kinase substrate p120cas binds directly to E-cadherin but not to the adenomatous polyposis coli protein or alpha- ... Li Z, Kim SH, Higgins JM, Brenner MB, Sacks DB (December 1999). "IQGAP1 and calmodulin modulate E-cadherin function". The ... calcium ion binding. • protein binding. • ankyrin binding. • gamma-catenin binding. • beta-catenin binding. • GTPase activating ...
... which leads to hyperpolarization and relaxation Stimulates a cGMP-dependent protein kinase that activates myosin light chain ... Nitric oxide is mediated in mammals by the calcium-calmodulin controlled isoenzymes eNOS (endothelial NOS) and nNOS (neuronal ... NOSs can be dimeric, calmodulin-dependent or calmodulin-containing cytochrome p450-like hemoprotein that combines reductase and ... which is linked in the middle of the protein to a calmodulin-binding domain. Binding of calmodulin appears to act as a " ...
see MAP kinase pathway. Calcium. *Intracellular calcium-sensing proteins. *Calcineurin. *Ca2+/calmodulin-dependent protein ... protein heterotrimerization. • Wnt signaling pathway, calcium modulating pathway. • protein folding. • G-protein coupled ... protein complex binding. • signal transducer activity. • protein binding. • GTPase activity. • GTPase binding. • G-protein ... 1omw: Crystal Structure of the complex between G Protein-Coupled Receptor Kinase 2 and Heterotrimeric G Protein beta 1 and ...
... is phosphorylated by the calcium-calmodulin-dependent protein kinase.[27] MAPKAPK2 (mitogen-activated-protein kinase-activating ... that are phosphorylated by a variety of protein kinases.[12][25] Ser40 is phosphorylated by the cAMP-dependent protein kinase.[ ... "Molecular cloning of cDNA coding for brain-specific 14-3-3 protein, a protein kinase-dependent activator of tyrosine and ... "Phosphorylation of tyrosine hydroxylase by calmodulin-dependent multiprotein kinase". The Journal of Biological Chemistry. 259 ...
see MAP kinase pathway. Calcium. *Intracellular calcium-sensing proteins. *Calcineurin. *Ca2+/calmodulin-dependent protein ... Types of G protein signaling[edit]. G protein can refer to two distinct families of proteins. Heterotrimeric G proteins, ... G proteins, also known as guanine nucleotide-binding proteins, are a family of proteins that act as molecular switches inside ... Heterotrimeric G proteins[edit]. Main article: Heterotrimeric G proteins. Different types of heterotrimeric G proteins share a ...
... but most prominently by Rho-dependent kinase and/or by the calcium-calmodulin-dependent myosin light chain kinase, not only ... protein binding. • ADP binding. • protein membrane anchor. • actin-dependent ATPase activity. • calmodulin binding. • ATPase ... Other proteins that are known to interact with NM IIA include the actin binding protein tropomyosin 4.2 [26] and a novel actin ... It encodes a protein of the same length, with 97.1% amino acid identity with the human MYH9 protein.[15] ...
It was regulated by Ca2+ via a calmodulin kinase II-dependent mechanism. Distant homologues may be present in plants, ciliates ... demonstrated that CLIC proteins have glutaredoxin-like glutathione-dependent oxidoreductase enzymatic activity. CLICs 1, 2 and ... Agnel, M.; Vermat, T.; Culouscou, J. M. (1999-07-23). "Identification of three novel members of the calcium-dependent chloride ... The proteins are formed from two copies of the same protein-a homodimer-though scientists have artificially combined subunits ...
Calcium binds to proteins such as calmodulin (CaM) and an eye-specific protein kinase C (PKC) known as InaC. These proteins ... Sepia colored eyes are not dependent on the sex of the fly. The Sepia eye color decreases sexual activity in males and ... In addition, proteins called arrestins bind metarhodopsin and prevent it from activating more Gq. A sodium-calcium exchanger ... For example, disruption of the interaction between InaC, the protein kinase C, and InaD results in a delay in inactivation of ...
... in which calcium-modulated protein calmodulin (CaM) binds Ca2+, undergoes a change in conformation, and activates CaM kinase II ... Phosphorylation by cAMP-dependent protein kinasesEdit. Cyclic AMP-dependent protein kinases (protein kinase A) are activated by ... The G protein-coupled receptor kinases (GRKs) are protein kinases that phosphorylate only active GPCRs.[53] G-protein-coupled ... stimulative regulative G-protein (Gs) or inhibitory regulative G-protein (Gi); adenylyl cyclase; protein kinase A (PKA); and ...
see MAP kinase pathway. Calcium. *Intracellular calcium-sensing proteins. *Calcineurin. *Ca2+/calmodulin-dependent protein ... Low concentrations of calcium cause the dimerization of RETGC-1 proteins through stimulation from guanylate cyclase-activating ... Guanylate cyclase is often part of the G protein signaling cascade that is activated by low intracellular calcium levels and ... This causes less intracellular calcium, which stimulates guanylate cyclase-activating proteins (GCAPs). Studies have shown that ...
... protein kinase Cs, calmodulin-modulated myosin light chain kinase, RAF/MEK/Mitogen-activated protein kinases, PKC/Ca2+/ ... G-protein coupled receptor signaling pathway. • inflammatory response. • positive regulation of cytosolic calcium ion ... stimulates adenyl cyclase to raise intracellular levels of cAMP and thereby activate cAMP-regulated protein kinases A-dependent ... that phosphorylate and thereby regulate target proteins involved in cell signaling (see Protein kinase C#Function). Studies ...
"The tyrosine kinase substrate p120cas binds directly to E-cadherin but not to the adenomatous polyposis coli protein or alpha- ... Li Z, Kim SH, Higgins JM, Brenner MB, Sacks DB (December 1999). "IQGAP1 and calmodulin modulate E-cadherin function". J. Biol. ... Jiang MC, Liao CF, Tai CC (June 2002). "CAS/CSE 1 stimulates E-cadhrin-dependent cell polarity in HT-29 human colon epithelial ... A proteína é unha glicoproteína de adhesión célula-célula dependente do calcio composta de cinco repeticións de cadherina ...
... activation of Ca2+/calmodulin-dependent protein kinase II can be cAMP dependent or independent.[15] ... increasing intracellular calcium among other cAMP mediated processes. The D2 class of receptors produce the opposite effect, as ... cAMP mediated protein kinase A activity also results in the phosphorylation of DARPP-32, an inhibitor of protein phosphatase 1 ... D2 receptor signaling may mediate protein kinase B, arrestin beta 2, and GSK-3 activity, and inhibition of these proteins ...
... the microtubule-associated tau proteins and S100b regulate tau phosphorylation by the Ca2+/calmodulin-dependent protein kinase ... calcium-dependent protein binding. • protein binding. • RAGE receptor binding. • identical protein binding. • tau protein ... S100 calcium-binding protein B (S100B) is a protein of the S-100 protein family. ... the microtubule-associated tau proteins and S100b regulate tau phosphorylation by the Ca2+/calmodulin-dependent protein kinase ...
see MAP kinase pathway. Calcium. *Intracellular calcium-sensing proteins. *Calcineurin. *Ca2+/calmodulin-dependent protein ... function of cAMP-dependent protein kinase. In humans, cAMP works by activating protein kinase A (PKA, cAMP-dependent protein ... an enzyme called protein kinase A (PKA).[12]. The PKA enzyme is also known as cAMP-dependent enzyme because it gets activated ... "Multiple pathway signal transduction by the cAMP-dependent protein kinase". FASEB J. 8 (15): 1227-36. doi:10.1096/fasebj.8.15. ...
... the cAMP-liver kinase B1 pathway, and the calcium/calmodulin-dependent protein kinase pathway.[6] A deficiency in any of these ... Luo, Y.; Raible, D.; Raper, J. A. (1993-10-22). "Collapsin: a protein in brain that induces the collapse and paralysis of ... as they must compete for the same proteins.[11] This causes the other neurites to develop into dendrites as they lack ... Tau proteins can aid in the stabilization of microtubules by binding to the microtubules, protecting them from microtubule ...
A calcium-calmodulin complex also acts to inhibit the binding of cAMP to the cAMP-dependent channel, thus contributing to ... in that cAMP works by directly binding to the ion channel rather than through activation of protein kinase A. It is similar to ... In mammals, the odorants stimulate adenylate cyclase to synthesize cAMP via a G protein called Golf. cAMP, which is the second ... A modern demonstration of that theory was the cloning of olfactory receptor proteins by Linda B. Buck and Richard Axel (who ...
see MAP kinase pathway. Calcium. *Intracellular calcium-sensing proteins. *Calcineurin. *Ca2+/calmodulin-dependent protein ... Cyclins, when bound with the dependent kinases, such as the p34/cdc2/cdk1 protein, form the maturation-promoting factor. MPFs ... is a family of proteins that control the progression of cells through the cell cycle by activating cyclin-dependent kinase (CDK ... Nurse won the 2001 Nobel Prize in Physiology or Medicine for their discovery of cyclin and cyclin-dependent kinase.[17] ...
... which then binds calmodulin. The activated calmodulin molecule activates myosin light-chain kinase (MLCK), which phosphorylates ... Voltage-gated calcium channels (VGCCs), also known as voltage-dependent calcium channels (VDCCs), are a group of voltage-gated ... The intracellular β subunit (55 kDa) is an intracellular MAGUK-like protein (Membrane-Associated Guanylate Kinase) containing a ... opening of the L-type calcium channel permits influx of calcium into the cell. The calcium binds to the calcium release ...
... calcium/calmodulin-dependent protein kinase IV, and the peroxisome proliferator γ coactivator 1 (PGC-1). The Ras/MAPK signaling ... proteins and other regulatory proteins. Ca2+/calmodulin-dependent protein kinase activity is also upregulated by slow motor ... calcineurin and calcium/calmodulin-dependent protein kinase IV to activate transcription factors that regulate gene expression ... Thus-through functional genomics-calcineurin, calmodulin-dependent kinase, PGC-1α, and activated PPARδ form the basis of a ...
Soderling TR (1999). „The Ca-calmodulin-dependent protein kinase cascade". Trends in Biochemical Sciences. 24 (6): 232-6. PMID ... Protein kinaza C (EC 2.7.11.13). Protein kinaza C, Protein kinaza Cζ, PKC alfa, PRKCB1, PRKCD, PRKCE, PRKCH, PRKCG, PRKCI, ... Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley- ... Receptor protein serin/treonin kinaza (EC 2.7.11.30). Koštani morfogenetski proteinski receptori (BMPR1, BMPR1A, BMPR1B, BMPR2 ...
Protein KinasesProtein-Serine-Threonine KinasesCalcium-Calmodulin-Dependent Protein KinasesCalcium-Calmodulin-Dependent Protein ... Calcium-Calmodulin-Dependent Protein Kinase Type 2 delta Subunit. *Calcium Calmodulin Dependent Protein Kinase Type 2 delta ... Calcium-Calmodulin-Dependent Protein Kinase Type 2 alpha Subunit. *Calcium Calmodulin Dependent Protein Kinase Type 2 alpha ... Calcium-Calmodulin-Dependent Protein Kinase Type 2 beta Subunit. *Calcium Calmodulin Dependent Protein Kinase Type 2 beta ...
One of the major mediators of calcium action in neurons is the multifunctional calcium/calmodulin-dependent protein kinase (CaM ... Inhibition of calcium/calmodulin-dependent protein kinase in Drosophila disrupts behavioral plasticity.. Griffith LC1, Verselis ... To assess the involvement of CaM kinase in experience-dependent behavior in an intact animal, we have designed a specific ... Protein Kinase Inhibitors. *Protein Kinases. *Calcium-Calmodulin-Dependent Protein Kinases. Grant support. *NS08779/NS/NINDS ...
View protein in PROSITE. PS00107, PROTEIN_KINASE_ATP, 1 hit. PS50011, PROTEIN_KINASE_DOM, 1 hit. PS00108, PROTEIN_KINASE_ ... View protein in PROSITE. PS00107, PROTEIN_KINASE_ATP, 1 hit. PS50011, PROTEIN_KINASE_DOM, 1 hit. PS00108, PROTEIN_KINASE_ ... Calcium/calmodulin-dependent protein kinaseARBA annotation. Automatic assertion according to rulesi ... tr,F1MVF1,F1MVF1_BOVIN Calcium/calmodulin-dependent protein kinase OS=Bos taurus OX=9913 GN=CAMK2G PE=3 SV=3 ...
Hunting Increases Phosphorylation of Calcium/Calmodulin-Dependent Protein Kinase Type II in Adult Barn Owls. Grant S. Nichols ... Here we investigated phosphorylation of calcium/calmodulin-dependent protein kinase II (pCaMKII) in both juveniles and adults. ... We previously demonstrated that phosphorylation of the cyclic-AMP response element-binding protein (CREB) provides a readout of ...
Hunting Increases Phosphorylation of Calcium/Calmodulin-Dependent Protein Kinase Type II in Adult Barn Owls. Grant S. Nichols ... A. Rodriguez-Contreras, X.-B. Liu, and W. M. DeBello, "Axodendritic contacts onto calcium/calmodulin-dependent protein kinase ... A. Hudmon and H. Schulman, "Neuronal CA2+/calmodulin-dependent protein kinase II: the role of structure and autoregulation in ... "Deficient hippocampal long-term potentiation in alpha-calcium-calmodulin kinase II mutant mice," Science, vol. 257, no. 5067, ...
Translocation of autophosphorylated calcium/calmodulin-dependent protein kinase II to the postsynaptic density. J. Biol. Chem. ... Calcium/calmodulin-dependent protein kinase II links ER stress with Fas and mitochondrial apoptosis pathways. Jenelle M. ... Regulation and role of brain calcium/calmodulin-dependent protein kinase II. Neurochem. Int. 21:469-497. View this article via ... Ca2+/calmodulin-dependent protein kinase II is required for microcystin-induced apoptosis. J. Biol. Chem. 277:2804-2811. View ...
... and CaMKII alpha and beta protein levels. In the hippocampus of Pb2+-exposed 50-day-old rats known to exhib ... we examined whether calcium/calmodulin-dependent protein kinase II (CaMKII) is affected by chronic developmental Pb2+ exposure ... Calcium/calmodulin dependent protein kinase II (CaMKII); NMDA receptor (NMDAR); Lead (Pb2+); Calcium signaling; Enzyme kinetics ... Calcium/calmodulin-dependent protein kinase II activity and expression are altered in the hippocampus of Pb2+- exposed rats.. ...
Calmodulin Trapping by Calcium-Calmodulin-Dependent Protein Kinase. By Tobias Meyer, Phyllis I. Hanson, Lubert Stryer, Howard ... Calmodulin Trapping by Calcium-Calmodulin-Dependent Protein Kinase. By Tobias Meyer, Phyllis I. Hanson, Lubert Stryer, Howard ... Calmodulin Trapping by Calcium-Calmodulin-Dependent Protein Kinase Message Subject. (Your Name) has forwarded a page to you ... Multifunctional calcium-calmodulin-dependent protein kinase (CaM kinase) transduces transient elevations in intracellular ...
Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro ... View protein in PROSITE. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 ... View protein in PROSITE. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 ... Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro ...
Calcium/calmodulin-dependent protein kinase II (CaMKII), a serine/threonine protein kinase, is well positioned to serve a role ... calcium/calmodulin-dependent protein kinase II;. PPF,. paired-pulse facilitation;. EPSC,. excitatory postsynaptic currents;. ES ... A significant fraction of the total calcium/calmodulin-dependent protein kinase II (CaMKII) activity in neurons is associated ... Essential function of α-calcium/calmodulin-dependent protein kinase II in neurotransmitter release at a glutamatergic central ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex ... Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation ... Calcium/calmodulin-dependent protein kinase type II subunit beta - Q13554 (KCC2B_HUMAN) ... The CAMK2 protein kinases contain a unique C-terminal subunit association domain responsible for oligomerization. UniProt ...
CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE TYPE II DELTA CHAIN. A, B. 301. Homo sapiens. Mutation(s): 0 Gene Names: CAMK2D, ... Crystal Structure of Human Calcium Calmodulin dependent Protein Kinase II delta isoform 1, CAMKD. *DOI: 10.2210/pdb2VN9/pdb ... LTP triggers high-frequency calcium pulses that result in the activation of Calcium/Calmodulin (CaM)-dependent kinase II ( ... LTP triggers high-frequency calcium pulses that result in the activation of Calcium/Calmodulin (CaM)-dependent kinase II ( ...
Calcium-Calmodulin-Dependent Protein Kinases*Calcium-Calmodulin-Dependent Protein Kinases. *Protein Kinases, Calcium-Calmodulin ... Multifunctional Calcium-Calmodulin-Dependent Protein Kinases*Multifunctional Calcium-Calmodulin-Dependent Protein Kinases ... Restricted Calcium-Calmodulin-Dependent Protein Kinases*Restricted Calcium-Calmodulin-Dependent Protein Kinases ... Protein-Serine-Threonine Kinases [D08.811.913.696.620.682.700]. *Calcium-Calmodulin-Dependent Protein Kinases [D08.811.913.696. ...
anti-Calcium/calmodulin-Dependent Protein Kinase I Antikörper * anti-Calcium/calmodulin-Dependent Protein Kinase (CaM Kinase) ... anti-Calcium/calmodulin-Dependent Protein Kinase IV Antikörper * anti-Calcium/calmodulin-Dependent Protein Kinase Kinase 1, ... calcium/calmodulin-dependent protein kinase type 1D , caM kinase ID , calcium/calmodulin-dependent protein kinase 1D , camKI- ... like protein kinase , mCKLiK , calcium/calmodulin-dependent protein kinase ID , calcium/calmodulin-dependent protein kinase ...
... calcium/calmodulin dependent protein kinase kinase 2), Authors: Dessen P. Published in: Atlas Genet Cytogenet Oncol Haematol. ... calcium/calmodulin-dependent protein kinase kinase beta. HGNC Previous name. calcium/calmodulin-dependent protein kinase kinase ... phosphorylation calmodulin-dependent protein kinase activity protein tyrosine kinase activity calcium ion binding calmodulin ... phosphorylation calmodulin-dependent protein kinase activity protein tyrosine kinase activity calcium ion binding calmodulin ...
The Medicago truncatula DMI3 gene encodes a calcium- and calmodulin-dependent protein kinase (CCaMK) that is necessary for the ... The Medicago truncatula DMI3 gene encodes a calcium- and calmodulin-dependent protein kinase (CCaMK) that is necessary for the ... acting like a switch able both to discriminate between rhizobial and mycorrhizal calcium signatures and to trigger the ... indicating that CCaMKs from nonlegumes can interpret the calcium signature elicited by rhizobial Nod factors and activate the ...
Park IK, Soderling TR: Activation of Ca2+/calmodulin-dependent protein kinase (CaM-kinase) IV by CaM-kinase kinase in Jurkat T ... Enslen H, Soderling TR: Roles of calmodulin-dependent protein kinases and phosphatase in calcium-dependent transcription of ... Calcium/calmodulin-dependent protein kinase types II and IV differentially regulate CREB-dependent gene expression. Mol Cell ... molecular cloning and expression of calcium/calmodulin-dependent protein kinase kinase. J Biol Chem270 :19320 -19324,1995. ...
The multifunctional calcium/calmodulin-dependent protein kinase II (CaMKII) is activated by catecholamines and angiotensin II, ... Aldosterone Enhances Cardiac Rupture After Myocardial Infarction Through Calcium/Calmodulin-Dependent Protein Kinase II. B. ... Aldosterone Enhances Cardiac Rupture After Myocardial Infarction Through Calcium/Calmodulin-Dependent Protein Kinase II ... Aldosterone Enhances Cardiac Rupture After Myocardial Infarction Through Calcium/Calmodulin-Dependent Protein Kinase II ...
Differential and Time-Dependent Changes in Gene Expression for Type II Calcium/Calmodulin-Dependent Protein Kinase, 67 kDa ... Differential and Time-Dependent Changes in Gene Expression for Type II Calcium/Calmodulin-Dependent Protein Kinase, 67 kDa ... Differential and Time-Dependent Changes in Gene Expression for Type II Calcium/Calmodulin-Dependent Protein Kinase, 67 kDa ... Differential and Time-Dependent Changes in Gene Expression for Type II Calcium/Calmodulin-Dependent Protein Kinase, 67 kDa ...
2004) Protein phosphatases and calcium/calmodulin-dependent protein kinase II-dependent synaptic plasticity. J Neurosci 24:8404 ... Calcium/calmodulin-dependent protein kinase II (CaMKII) is a highly expressed serine/threonine kinase whose α and β isoforms ... 2003) Calcium/calmodulin-dependent protein kinase II contributes to activity-dependent filopodia growth and spine formation. J ... 2005) Multivalent interactions of calcium/calmodulin-dependent protein kinase II with the postsynaptic density proteins NR2B, ...
6-thiadiazin-4-one bound to calcium/calmodulin-dependent protein kinase kinase 2 (CaMKK2), several analogues were identified ... they represent starting points for development of highly selective kinase inhibitors. ... can function as a chemotype for the design of ATP-competitive kinase inhibitors. Using insights from a co-crystal structure of ... 1,2,6-Thiadiazinones as Novel Narrow Spectrum Calcium/Calmodulin-Dependent Protein Kinase Kinase 2 (CaMKK2) Inhibitors by ...
Calcium/calmodulin-dependent protein kinase II and calcium/phospholipid-dependent protein kinase activities in rat tissues ... Calcium signaling through protein kinases. The Arabidopsis calcium-dependent protein kinase gene family. Plant Physiol 129: 469 ... Chimeric plant calcium/calmodulin-dependent protein kinase gene with a neural visinin-like calcium-binding domain. Proc Natl ... Chimeric calcium/calmodulin-dependent protein kinase in tobacco: differential regulation by calmodulin isoforms. Plant Mol Biol ...
Calcium-activated Potassium Channels via Activating Calmodulin-dependent Protein Kinase Kinase and AMP-activated Protein Kinase ... Calcium-activated Potassium Channels via Activating Calmodulin-dependent Protein Kinase Kinase and AMP-activated Protein Kinase ... Calcium-activated Potassium Channels via Activating Calmodulin-dependent Protein Kinase Kinase and AMP-activated Protein Kinase ... Calcium-activated Potassium Channels via Activating Calmodulin-dependent Protein Kinase Kinase and AMP-activated Protein Kinase ...
Expression of a Ca2+/calmodulin-dependent protein kinase, CaM kinase-Gr, in human T lymphocytes. Regulation of kinase activity ... Calcium/calmodulin-dependent protein kinase IV (CaMK4), a serine/threonine kinase expressed in T cells (6), regulates the ... calcium/calmodulin-dependent protein kinase IV. CREM. cAMP responsive element modulator. SLE. systemic lupus erythematosus. ... Calcium/Calmodulin-Dependent Protein Kinase IV Suppresses IL-2 Production and Regulatory T Cell Activity in Lupus. Tomohiro ...
Protein (His tag). Spezies: Human. Quelle: Escherichia coli (E. coli). Jetzt Produkt ABIN668012 bestellen. ... Recombinant Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4) ... Calcium/calmodulin-Dependent Protein Kinase Kinase 1, alpha Proteine * Calcium/calmodulin-Dependent Protein Kinase Kinase 2, ... Calcium/calmodulin-Dependent Protein Kinase I Proteine * Calcium/calmodulin-Dependent Protein Kinase (CaM Kinase) II beta ...
Showing Protein Calcium/calmodulin-dependent protein kinase isoform A (HMDBP09421). IdentificationBiological propertiesGene ... Protein Sequence. ,Calcium/calmodulin-dependent protein kinase isoform A ... Cloning and analysis of two new isoforms of multifunctional Ca2+/calmodulin-dependent protein kinase. Expression in multiple ... protein binding. protein kinase activity. protein serine/threonine kinase activity. calmodulin-dependent protein kinase ...
Calcium can function as a second messenger through stimulation of calcium-dependent protein kinases. A protein kinase that ... A calcium-dependent protein kinase with a regulatory domain similar to calmodulin ... A calcium-dependent protein kinase with a regulatory domain similar to calmodulin ... A calcium-dependent protein kinase with a regulatory domain similar to calmodulin ...
Calcium and calmodulin-dependent protein kinase II, through phosphorylation of the ryanodine receptor would lead to Ca2+ leak ... Pharmacologic inhibition of calcium and calmodulin-dependent protein kinase II with 2.5 µM of KN93 prevented the decrease in ... Calcium/Calmodulin Protein Kinase II-Dependent Ryanodine Receptor Phosphorylation Mediates Cardiac Contractile Dysfunction ... Development of organ-specific calcium and calmodulin-dependent protein kinase II inhibitors may result in a beneficial ...
CaMKII (Calcium/calmodulin-dependent protein kinase II) is a ubiquitous serine/threonine protein kinase that is abundant in the ... CaMKII is a prominent kinase in the central nervous system that may function in long term potentiation and neurotransmitter ... brain as a major constituent of the postsynaptic density (PSD). The enzyme is an oligomeric protein composed of distinct but ... spatial learning and memory decline in C57BL/6 J mice by regulating hippocampal cyclic amp-response element binding protein ...
It was recently shown that an autoactive form of a nodulation-specific calcium/calmodulin-dependent protein kinase is ... We show here that MtNIN is essential for autoactive calcium/calmodulin-dependent protein kinase-induced nodule organogenesis. ... Mitra RM, Gleason CA, Edwards A, Hadfield J, Downie JA, Oldroyd GED, Long SR (2004a) A Ca2+/calmodulin-dependent protein kinase ... the information it encodes is believed to be accomplished through a plant-specific calcium/calmodulin-dependent protein kinase ...
  • A cDNA encoding a calcium (Ca 2+ )/calmodulin (CaM)-dependent protein kinase (CaMK) from tobacco ( Nicotiana tabacum ), NtCaMK1 , was isolated by protein-protein interaction-based screening of a cDNA expression library using 35 S-labeled CaM as a probe. (plantphysiol.org)
  • several protein kinases that share sequence homology with CaMK have been identified in plants. (plantphysiol.org)
  • 4,5 As part of the β 1 -adrenergic pathway, calcium/calmodulin-dependent protein kinase II (CaMK II) is a serine/threonine kinase that is involved in intracellular Ca 2+ handling by phosphorylating various Ca 2+ handling proteins, including phospholamban and ryanodine receptors. (asahq.org)
  • Incubation of rat brain frontal cortical slices with 6-chloro-7,8-dihydroxy-3-methyl-1-(3-methylphenyl)-2,3,4,5-tetrahydro-1 H -3-benzazepine (SKF83959), a recently identified selective agonist of the PI-linked D 1 -like dopamine receptor, elicited transient time- and dose-dependent stimulations of cyclin-dependent kinase 5 (cdk5) and calcium/calmodulin-dependent protein kinase II (CaMK II) activities. (aspetjournals.org)
  • Moreover, we found that cdk5 phosphorylates dopamine and cAMP-regulated phosphoprotein at Thr75, whereas CaMK II is responsible for the activation of cAMP response element-binding protein in response to SKF83959 stimulation. (aspetjournals.org)
  • Ca 2+ /calmodulin regulates CaMK, a serine/threonine protein kinase. (aspetjournals.org)
  • The neuroprotective mechanisms of the Ca2+/calmodulin kinase (CaMK) signaling pathway were studied in primary cerebellar neurons in vitro. (semanticscholar.org)
  • We previously showed that Plasmodium falciparum expresses a homolog of human calcium calmodulin-dependent protein kinase (CaMK) that is important for host cell invasion. (biomedcentral.com)
  • In this study, to identify novel targets for the treatment of Toxoplasma gondii infection (another apicomplexan parasite), we sought to identify a CaMK-like protein in the T. gondii genome and to characterize its role in the life-cycle of this parasite. (biomedcentral.com)
  • An in vitro kinase assay was performed to assess the phosphorylation activities of a novel CaMK-like protein in T. gondii by using purified proteins with various concentrations of calcium, calmodulin antagonists, or T. gondii glideosome proteins. (biomedcentral.com)
  • We identified a novel CaMK homolog in T. gondii , T. gondii CaMK-related kinase (TgCaMKrk), which exhibits calmodulin-independent autophosphorylation and substrate phosphorylation activity. (biomedcentral.com)
  • Here, we found that calcium / calmodulin -dependent protein kinase (CaM kinase , CaMK) is involved in HMGB1 release by regulating IFN-beta production . (bvsalud.org)
  • To investigate the effects of valsartan on myocardial expression and activity of calcium / calmodulin -dependent protein kinase -II (CaMK II) in a rabbit model of heart failure . (bvsalud.org)
  • In the present study, we examined whether calcium/calmodulin-dependent protein kinase II (CaMKII) is affected by chronic developmental Pb2+ exposure. (cdc.gov)
  • The effects of Pb2+ exposure on rat hippocampal CaMKII were assessed by measuring CaMKII activity, phosphorylation of CaMKII at threonine-286, and CaMKII alpha and beta protein levels. (cdc.gov)
  • These Pb2+-induced changes in CaMKII activity could not be explained by changes in enzyme phosphorylation at threonine-286 or sensitivity to calmodulin. (cdc.gov)
  • Western blots of cytosolic and membrane fractions from hippocampus showed a significant decrease in the levels of CaMKII-beta but not alpha protein in the cytosolic fraction of Pb2+-exposed rats. (cdc.gov)
  • These findings indicate effects of developmental Pb2+ exposure on CaMKII, a component of calcium signaling associated with synaptic plasticity, learning, and memory. (cdc.gov)
  • A significant fraction of the total calcium/calmodulin-dependent protein kinase II (CaMKII) activity in neurons is associated with synaptic connections and is present in nerve terminals, thus suggesting a role for CaMKII in neurotransmitter release. (pnas.org)
  • Conditional CA3 α-CaMKII knockout mice exhibited an unchanged basal probability of neurotransmitter release at CA3-CA1 synapses but showed a significant enhancement in the activity-dependent increase in probability of release during repetitive presynaptic stimulation, as was shown with the analysis of unitary synaptic currents. (pnas.org)
  • These data indicate that α-CaMKII serves as a negative activity-dependent regulator of neurotransmitter release at hippocampal synapses and maintains synapses in an optimal range of release probabilities necessary for normal synaptic operation. (pnas.org)
  • Calcium/calmodulin-dependent protein kinase II (CaMKII), a serine/threonine protein kinase, is well positioned to serve a role in synaptic function regulation, because it is highly expressed in the brain and is known to phosphorylate multiple synaptic proteins ( 2 ). (pnas.org)
  • Accordingly, CaMKII has previously been implicated in the long-lasting frequency-dependent regulation of synaptic function in mammals ( 3 - 9 ), birds ( 10 ), frogs ( 11 ), and invertebrates ( 12 ). (pnas.org)
  • CaMKII acts as a molecular switch because it remains active for a long time after the return to basal calcium levels, which is a unique property required for CaMKII function. (rcsb.org)
  • Comparisons with autoinhibited CaMKII structures showed that binding of calmodulin leads to the rearrangement of residues in the active site to a conformation suitable for ATP binding and to the closure of the binding groove for the autoinhibitory helix by helix alphaD. (rcsb.org)
  • The structural data, together with biophysical interaction studies, reveals the mechanism of CaMKII activation by calmodulin and explains many of the unique regulatory properties of these two essential signaling molecules. (rcsb.org)
  • The multifunctional calcium/calmodulin-dependent protein kinase II (CaMKII) is activated by catecholamines and angiotensin II, and CaMKII inhibition prevents isoproterenol- and angiotensin II-mediated cardiomyopathy. (ahajournals.org)
  • To study potential molecular mechanisms of epileptogenesis in the neocortex, the motor cortex of rats was injected with tetanus toxin (TT), and gene expression for 67 kDa glutamic acid decarboxylase (GAD-67), type II calcium/calmodulin-dependent protein kinase (CaMKII), NMDA receptor subunit 1 (NR1), and AMPA receptor subunit 2 (GluR2) was investigated by in situ hybridization histochemistry. (jneurosci.org)
  • We examined gene expression for molecules involved in GABAergic and glutamatergic transmission in neocortex, namely type II Ca 2+ /calmodulin-dependent protein kinase (CaMKII), GAD, and the most highly expressed NMDA and AMPA receptor subunits, in the presence of focal epilepsy induced by TT injection. (jneurosci.org)
  • The transcription factor ΔFosB and the brain-enriched calcium/calmodulin-dependent protein kinase II (CaMKIIα) are induced in the nucleus accumbens (NAc) by chronic exposure to cocaine or other psychostimulant drugs of abuse, in which the two proteins mediate sensitized drug responses. (jneurosci.org)
  • Here, we demonstrate that ΔFosB is phosphorylated by CaMKIIα at the protein-stabilizing Ser27 and that CaMKII is required for the cocaine-mediated accumulation of ΔFosB in rat NAc. (jneurosci.org)
  • Furthermore, induction of dendritic spines on NAc MSNs and increased behavioral responsiveness to cocaine after NAc overexpression of ΔFosB are both CaMKII dependent. (jneurosci.org)
  • Among them, multifunctional CaMKII, a well-characterized kinase that can phosphorylate a broad range of proteins upon binding to Ca 2+ /CaM, is thought to play an important role in a variety of cellular events in mammals ( Colbran and Soderling, 1990 ). (plantphysiol.org)
  • CaMKII (Calcium/calmodulin-dependent protein kinase II) is a ubiquitous serine/threonine protein kinase that is abundant in the brain as a major constituent of the postsynaptic density (PSD). (neuromics.com)
  • CaMKII is a prominent kinase in the central nervous system that may function in long term potentiation and neurotransmitter release. (neuromics.com)
  • Ca(2+)/Calmodulin-dependent protein kinase II (CaMKII) signaling in the heart regulates cardiomyocyte contractility and growth in response to elevated intracellular Ca(2+). (escholarship.org)
  • CPCs undergoing early differentiation in vitro increase levels of CaMKIIδB in the nuclear compartment where the kinase may contribute to the regulation of CPC commitment. (escholarship.org)
  • Therefore, CaMKIIδB may serve as a novel modulatory protein to enhance CPC survival and commitment into the cardiac and smooth muscle lineages. (escholarship.org)
  • Further quantitative immunoblotting indicated that CaMKIId is highly expressed in the vasculature and initial results suggest some alterations in ox-CaMKII protein levels with ageing, parallel to that observed in the heart. (strath.ac.uk)
  • Recent evidence has shown that diabetic patients and mouse models of diabetes have an up-regulation of calmodulin-dependent protein kinase delta (CaMKIIδ) activity. (otago.ac.nz)
  • CaMKIIδ is a multifunctional serine-threonine kinase expressed as four different genes that generate four isoforms (αβγδ) and a number of splice variants. (otago.ac.nz)
  • Upon CaMKIIδ activation is involved in coordination of the ion channels and Ca2+ handling proteins involved in excitation-contraction and excitation-transcription coupling in the myocardium. (otago.ac.nz)
  • Calcium/calmodulin-dependent kinase II (CaMKII) forms a highly conserved dodecameric assembly that is sensitive to the frequency of calcium pulse trains. (nih.gov)
  • We present the crystal structure of an autoinhibited full-length human CaMKII holoenzyme, revealing an unexpected compact arrangement of kinase domains docked against a central hub, with the calmodulin-binding sites completely inaccessible. (nih.gov)
  • Comparison of CaMKII isoforms, which differ in the length of the linker between the kinase domain and the hub, demonstrates that these interactions can be strengthened or weakened by changes in linker length. (nih.gov)
  • One consequence of increased [Ca 2+ ] i would be altered activity and function of calcium-regulated proteins, including calcium-calmodulin-dependent protein kinase II (CaMKII), which is autophosphorylated on Thr 286 (pCaMKII 286 ) in the presence of calcium and calmodulin. (elsevier.com)
  • Aberrant calcium handling, including increased activation of calcium/calmodulin-dependent protein kinase II (CaMKII), contributes to arrhythmia development during HF. (elsevier.com)
  • The major binding species was a doublet at 50-60 kDa, which was identified as calcium/calmodulin-dependent protein kinase II (CaMKII) by N-terminal peptide analysis and confirmed by matrix-assisted laser desorption ionization-mass spectrometry as the δ- and β-like isoforms. (edu.au)
  • To investigate CaMKII involvement in insulin-stimulated glucose uptake, 3T3-L1 adipocytes were infected with retrovirus encoding green fluorescent protein (GFP)-hemagluttinin tag (HA)-tagged CaMKII wild-type or the ATP binding mutant, K42M. (edu.au)
  • GFP-HA-CaMKII K42M cells had less kinase activity than cells expressing wild-type GFP-HA-CaMKII. (edu.au)
  • HF has been demonstrated to increase the expression and function of calcium/calmodulin-dependent protein kinase II (CaMKII), a key regulator of calcium homeostasis and excitation-contraction coupling in cardiomyocytes. (purdue.edu)
  • Whole-cell, voltage-clamp patch clamp electrophysiology experiments were performed in HEK 293 cells transiently co-expressing wild-type (WT) or mutant KCNQ1 (mutations conferring mimics of dephosphorylation and phosphorylation were introduced at phosphorylation sites identified by LCMS/MS) and KCNE1 (auxiliary subunit) during ISO treatment, treatment with CaMKII or protein kinase A (PKA) inhibitors, or during lentiviral δCaMKII overexpression. (purdue.edu)
  • Protein immunoblot experiments were performed to (1) assess CaMKII activation during ISO treatment and (2) to assess plasma membrane expression of KCNQ1 and KCNE1 subunits with mimics of differential KCNQ1 phosphorylation following a membrane protein biotinylation procedure. (purdue.edu)
  • Calcium/calmodulin-dependent protein kinase IIα ( CamKIIα ) may modulate the function of mu-opioid receptors by phosphorylation and therefore, be involved in development of morphine-induced analgesic tolerance. (kowsarpub.com)
  • Calcium/calmodulin-dependent protein kinase type II (CamKII) is a serine/threonine kinase that constitutes a family of multifunctional protein kinases and plays a major role in Ca 2+- mediated signal transduction ( 14 ). (kowsarpub.com)
  • Once activated, CaMKII phosphorylates numerous target proteins and is involved in many cellular functions, including synaptic plasticity, synaptic vesicle mobilization, regulation of gene expression and modulation of ion channel function ( 14 , 20 ). (kowsarpub.com)
  • Inhibition of Ca 2+ /calmodulin-dependent kinase II (CaMKII) completely blocks the acute effect of NT3. (rupress.org)
  • A multifunctional calcium-calmodulin-dependent protein kinase subtype that occurs as an oligomeric protein comprised of twelve subunits. (nih.gov)
  • One of the major mediators of calcium action in neurons is the multifunctional calcium/calmodulin-dependent protein kinase (CaM kinase), an enzyme with the capability of directly regulating its own activity by autophosphorylation. (nih.gov)
  • Multifunctional calcium-calmodulin-dependent protein kinase (CaM kinase) transduces transient elevations in intracellular calcium into changes in the phosphorylation state and activity of target proteins. (sciencemag.org)
  • The multifunctional calcium/calmodulin-dependent protein kinase: from form to function. (semanticscholar.org)
  • It differs from other enzyme subtypes in that it lacks a phosphorylatable activation domain that can respond to CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASE KINASE. (nih.gov)
  • A CALMODULIN-dependent enzyme that catalyzes the phosphorylation of proteins. (harvard.edu)
  • This enzyme is also sometimes dependent on CALCIUM. (harvard.edu)
  • This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. (antikoerper-online.de)
  • The enzyme is an oligomeric protein composed of distinct but related subunits, alpha, beta, gamma, and delta, each encoded by a separate gene. (neuromics.com)
  • The stimulation by calcium of the particulate enzyme and of the partially purified cytosolic enzyme requires the addition of calmodulin. (caltech.edu)
  • The ELISA (enzyme-linked immunosorbent assay) is a widely used application for detecting and quantifying proteins and antigens from various samples. (biocompare.com)
  • Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b) in Tissue homogenates, cell lysates and other biological fluids. (unicoupi.com)
  • Maximal enhancement of CaM-kinase II activity was observed when autophosphorylated or Ca 2+ /CaM-bound forms of the enzyme were proteolyzed. (elsevier.com)
  • The Kinase Enzyme Systems allow you to easily screen and profile kinase inhibitors. (promega.com)
  • They provide all the optimized components (enzyme, preferred substrate, required cofactors, buffer) that you need to generate a kinase selectivity profile for a compound. (promega.com)
  • Below each tab is a list of currently available Kinase Enzyme Systems (or those coming soon). (promega.com)
  • Click on any enzyme for additional information (such as Applications Note, the Catalog Page, or the NCBI Database entry) for that kinase. (promega.com)
  • We demonstrate for the first time that 4 H -1,2,6-thiadiazin-4-one (TDZ) can function as a chemotype for the design of ATP-competitive kinase inhibitors. (mdpi.com)
  • Since the TDZ analogues showed reduced promiscuity compared to their 2,4-dianilinopyrimidine counter parts, they represent starting points for development of highly selective kinase inhibitors. (mdpi.com)
  • Development of organ-specific calcium and calmodulin-dependent protein kinase II inhibitors may result in a beneficial therapeutic strategy to ameliorate contractile dysfunction associated with sepsis. (ovid.com)
  • A screen of 72 inhibitors against 456 human kinases. (guidetopharmacology.org)
  • 2010) Activation state-dependent binding of small molecule kinase inhibitors: structural insights from biochemistry. (guidetopharmacology.org)
  • A screen profiling 158 kinase inhibitors (Calbiochem Protein Kinase Inhibitor Library I and II, catalogue numbers 539744 and 539745) for their inhibitory activity at 1µM and 10µM against 234 human recombinant kinases using the EMD Millipore KinaseProfiler TM service. (guidetoimmunopharmacology.org)
  • A screen profiling the inhibitory activity of 178 commercially available kinase inhibitors at 0.5µM against a panel of 300 recombinant protein kinases using the Reaction Biology Corporation Kinase Hotspot SM platform. (guidetoimmunopharmacology.org)
  • We show that substrate phosphorylation and autophosphorylation of NtCaMK1 is Ca 2+ /CaM dependent. (plantphysiol.org)
  • The two cytosolic kinases are distinguishable from each other and from myosin light chain kinase and phosphorylase b kinase by their substrate specificities and their chromatographic behavior on DEAE-cellulose. (caltech.edu)
  • None of the mutants exhibited enhanced Ca 2+ -independent kinase activity toward exogenous substrate, but the K300S and N294S mutants showed a significant enhancement in the rate and stoichiometry of 32 P incorporation during Ca 2+ -independent autophosphorylation. (elsevier.com)
  • Each SignaTECT® System contains a highly specific biotinylated peptide substrate for the appropriate kinase as well as the necessary reaction components. (mybio.ie)
  • After the kinase reaction is complete, the biotinylated, 32 P-labeled substrate is recovered from the reaction mix using the SAM 2 ® Biotin Capture Membrane. (mybio.ie)
  • Substrate specificity of Ca(2+)/calmodulin-dependent protein kinase phosphatase: kinetic studies using synthetic phosphopeptides as model substrates. (semanticscholar.org)
  • These results indicate that the auto-inhibitory domain in CaMKI gates, in a Ca(2+)-CaM dependent fashion, accessibility of both substrates to the substrate binding cleft and CaMKI kinase to Thr177. (duke.edu)
  • The multifunctional calcium/calmodulin-dependent protein kinases I and IV (CaMKI and CaMKIV) are closely related by primary sequence and predicted to have similar substrate specificities based on peptide studies. (duke.edu)
  • We identified a fragment of p300-(1-117) that is a substrate of both kinases, and through both mutagenesis and Edman phosphate ((32)P) release sequencing, established that CaMKI and CaMKIV phosphorylate completely different sites. (duke.edu)
  • To compare kinase substrate preferences more generally, we employed a proteomic display technique that allowed comparison of complex cell extracts phosphorylated by each kinase in a rapid in vitro assay, thereby demonstrating substrate preferences that overlapped but were clearly distinct. (duke.edu)
  • To validate this approach, one of the proteins labeled in this assay was identified by microsequencing as HSP25, purified as a recombinant protein, and examined as a substrate for both CaMKI and CaMKIV. (duke.edu)
  • These differences in substrate specificities emphasize the need to consider these protein kinases independently despite their close homology. (duke.edu)
  • Phosphorylated synthetic peptides as tools for studying protein phosphatases. (semanticscholar.org)
  • Inhibition of calcium/calmodulin-dependent protein kinase in Drosophila disrupts behavioral plasticity. (nih.gov)
  • Pharmacologic inhibition of calcium and calmodulin-dependent protein kinase II with 2.5 µM of KN93 prevented the decrease in cell shortening, Ca 2+ transient amplitude, and sarcoplasmic reticulum Ca 2+ content in colon ascendens stent peritonitis myocytes. (ovid.com)
  • Our previous data has suggested that CaMKK β is neuroprotective after stroke in young mice as inhibition of this kinase aggravated stroke outcome. (ahajournals.org)
  • The calcium stimulation of this latter kinase is probably mediated by calmodulin, judging from its inhibition by low concentrations of trifluoperazine. (caltech.edu)
  • [9] As well as preventing methamphetamine (Meth) induced neurotoxicity via inhibition of nitric oxide synthase (nNOS) expression and astrocyte activation, is seen to reduce methamphetamine induced brain damage through a CB1-dependent and independent mechanisms, respectively, and inhibition of methamphetamine induced astrogliosis is likely to occur through a CB2 receptor dependent mechanism for THC . (wikipedia.org)
  • Furthermore, CPK28 autophosphorylation substantially reduced sensitivity of the kinase to Ca 2+ /CaM inhibition. (illinois.edu)
  • Another source of smooth muscle disorders like ischemia-reperfusion, hypertension, and coronary artery disease arise when mutations to protein kinase C (PKC) result in excessive inhibition of MYLP, which counteracts the activity of MYLK by dephosphorylating myosin light chain. (wikipedia.org)
  • It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. (antibodies-online.com)
  • Protein kinases catalyze phosphate transfer from Adenosine Triphosphate (ATP) to tyrosine, threonine or serine residues in specific target proteins. (mdpi.com)
  • Lorecivivint, a Novel Intraarticular CDC-Like Kinase 2 and Dual-Specificity Tyrosine Phosphorylation-Regulated Kinase 1A Inhibitor and Wnt Pathway Modulator for the Treatment of Knee Osteoarthritis: A Phase II Randomized Trial. (escholarship.org)
  • GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity . (genecards.org)
  • Stimulated by its physiological ligand, hepatocyte growth factor, the transmembrane receptor tyrosine kinase Met activates a signaling machinery that leads to mitogenic, motogenic, and morphogenic responses. (mcponline.org)
  • The horseshoe-like shape of InlB 321 allows binding to and activation of the transmembrane tyrosine kinase Met, which is also the receptor for the host growth factor, hepatocyte growth factor (HGF). (mcponline.org)
  • Receptors include seven-pass transmembrane Frizzleds and receptor tyrosine kinases ROR and Ryk. (antibodies-online.com)
  • 30% and phosphoserine-mediated transcriptional activity by 58% but has no effect on phosphorylation of Y701 or receptor-associated JAK tyrosine kinases. (jimmunol.org)
  • Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. (uniprot.org)
  • Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. (genecards.org)
  • Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK1 signaling cascade and, upon calcium influx, regulates transcription activators activity, cell cycle, hormone production, cell differentiation, actin filament organization and neurite outgrowth. (abcam.com)
  • Calcium regulation of neuronal gene expression," Proceedings of the National Academy of Sciences of the United States of America , vol. 98, no. 20, pp. 11024-11031, 2001. (hindawi.com)
  • Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca(2+) transport in skeletal muscle. (rcsb.org)
  • The regulation of intracellular calcium content by IP 3 is critical in mediating neuronal responses. (aspetjournals.org)
  • Accumulating evidence indicates that long term-neuronal plasticity is dependent on specific patterns of gene expression evoked in response to stimuli. (pnas.org)
  • Olomoucine is known as a cyclin-dependent kinase inhibitor. (edu.au)
  • Three genes encoding different Ca2+/calmodulin-dependent protein kinases have been characterized in the wheat phytopathogenic fungus Stagonospora nodorum. (edu.au)
  • In the nucleus, phosphorylates the MEF2 repressor HDAC4, promoting its nuclear export and binding to 14-3-3 protein, and expression of MEF2 and genes involved in the hypertrophic program. (cusabio.com)
  • The CASK protein is primarily found in nerve cells (neurons) in the brain, where it helps control the activity (expression) of other genes that are involved in brain development. (medlineplus.gov)
  • This gene encodes a calcium/calmodulin-dependent serine protein kinase. (genecards.org)
  • CASK (Calcium/Calmodulin Dependent Serine Protein Kinase) is a Protein Coding gene. (genecards.org)
  • The CASK gene provides instructions for making a protein called calcium/calmodulin-dependent serine protein kinase (CASK). (medlineplus.gov)
  • Human genetic studies have indicated that mutations in calcium/calmodulin-dependent serine protein kinase ( CASK ) result in X-linked mental retardation and autism-spectrum disorders. (jpn.ca)
  • Up regulation of cAMP response element-mediated gene expression during experience-dependent plasticity in adult neocortex," Journal of Neuroscience , vol. 20, no. 11, pp. 4206-4216, 2000. (hindawi.com)
  • The existence of multiple divergent CaM isoforms in plants poses the question of whether or not they allow differential regulation of targets and can confer different Ca 2+ sensitivity to CaM-binding enzymes or proteins. (plantphysiol.org)
  • Aim Endothelial cell (EC) KCa2.3 and KCa3.1, calcium-activated potassium channels, play a crucial role in NO- and EDHF-mediated regulation of vascular tone by controlling membrane potential and intracellular calcium levels. (ahajournals.org)
  • The predicted structure of this kinase explains its direct regulation via calcium binding and establishes it as a prototype for a new family of calcium-regulated protein kinases. (sciencemag.org)
  • 2. Li Y, Spangenberg O, Paarmann I, Konrad M, Lavie A. (2002) Structural basis for nucleotide-dependent regulation of membrane-associated guanylate kinase-like domains. (guidetopharmacology.org)
  • Additional T. gondii protein kinases are involved in host manipulation, cell cycle regulation, and functions required for growth, stress responses, and the transition from tachyzoite to bradyzoite [ 15 ]. (biomedcentral.com)
  • Calcium/calmodulin-dependent protein kinase involved in the regulation of Ca(2+) homeostatis and excitation-contraction coupling (ECC) in heart by targeting ion channels, transporters and accessory proteins involved in Ca(2+) influx into the myocyte, Ca(2+) release from the sarcoplasmic reticulum (SR), SR Ca(2+) uptake and Na(+) and K(+) channel transport. (cusabio.com)
  • Garcia, Joe G N. / Regulation of endothelial cell barrier function by calcium/calmodulin-dependent protein kinase II . (elsevier.com)
  • A putative mechanism for persistent regulation of synaptic events by such a proteolytic activation of CaM-kinase 11 is discussed. (elsevier.com)
  • CPKs are composed of a dual specificity (Ser/Thr and Tyr) kinase domain tethered to a calmodulin-like domain (CLD) via an autoinhibitory junction (J). Although regulation of CPKs by Ca 2+ has been extensively studied, the contribution of autophosphorylation in controlling CPK activity is less well understood. (illinois.edu)
  • Furthermore, whether calmodulin (CaM) contributes to CPK regulation, as is the case for Ca 2+ /CaM-dependent protein kinases outside the plant lineage, remains an open question. (illinois.edu)
  • The encoded protein contains a high mobility group-box DNA binding domain and participates in the regulation of cell. (antibodies-online.com)
  • BRCA1 encodes a tumour suppressor protein that plays pivotal roles in homologous recombination (HR) DNA repair, cell-cycle checkpoints, and transcriptional regulation. (biomedsearch.com)
  • abstract = "Calcium/calmodulin-dependent protein kinase II delta (CaMKIId) plays a fundamental role in cardiac dysfunction and is known to be overexpressed and hyper-activated in the diseased heart. (strath.ac.uk)
  • abstract = "Thrombin-induced endothelial cell barrier dysfunction is tightly linked to Ca2+-dependent cytoskeletal protein reorganization. (elsevier.com)
  • abstract = "Rat forebrain Ca2+/calmodulin-dependent protein kinase II (CaM-kinase II) in isolated postsynaptic densities (PSD) was subjected to limited proteolysis with chymotrypsin or μ-calpain, a Ca2+-dependent protease. (elsevier.com)
  • Calcium/calmodulin-dependent protein kinase IV (CaMK4), a serine/threonine kinase expressed in T cells ( 6 ), regulates the activity of several transcription factors including CREM ( 7 ). (jimmunol.org)
  • Thus, these receptors regulate the levels of cAMP, which in turn regulates the activity of protein kinase A (PKA). (aspetjournals.org)
  • Ca(2+)/calmodulin-dependent protein kinase phosphatase (CaMKPase) dephosphorylates and regulates multifunctional Ca(2+)/calmodulin-dependent protein kinases. (semanticscholar.org)
  • Because Cask encodes a multidomain scaffold protein, a possible strategy to dissect how CASK regulates mental ability and cognition is to disrupt specific protein-protein interactions of CASK in vivo and then investigate the impact of individual specific protein interactions. (jpn.ca)
  • Similar to ROCK, Protein Kinase C regulates MYLK via the CPI-17 protein, which downregulates MYLP. (wikipedia.org)
  • CAMK4 belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. (antikoerper-online.de)
  • CAMK1D encodes a member of the Ca2+/calmodulin-dependent protein kinase 1 subfamily of serine/threonine kinases. (antikoerper-online.de)
  • These results suggest that CaM kinase activity is required for plastic behaviors in an intact animal. (nih.gov)
  • Calcium/calmodulin-dependent protein kinase II activity and expression are altered in the hippocampus of Pb2+- exposed rats. (cdc.gov)
  • CaM-KIVc alone was sufficient, and the upstream kinase, CaM-KK, was enhanced to upregulate the insulin promoter activity in INS-1 cells. (diabetesjournals.org)
  • Using insights from a co-crystal structure of a 3,5-bis(arylamino)-4 H -1,2,6-thiadiazin-4-one bound to calcium/calmodulin-dependent protein kinase kinase 2 (CaMKK2), several analogues were identified with micromolar activity through targeted displacement of bound water molecules in the active site. (mdpi.com)
  • The activity of calcium/calmodulin-dependent protein kinase IV (CaMK4) is increased in T cells from patients with systemic lupus erythematosus (SLE) and has been shown to reduce IL-2 production by promoting the effect of the transcriptional repressor cAMP responsive element modulator-α on the IL2 promoter. (jimmunol.org)
  • A protein kinase that requires calcium but not calmodulin or phospholipids for activity has been purified from soybean. (sciencemag.org)
  • Colon ascendens stent peritonitis hearts showed a significant increase in oxidation-dependent calcium and calmodulin-dependent protein kinase II activity, which could be prevented by pretreating animals with the antioxidant tempol. (ovid.com)
  • When calcium/calmodulin-dependent calmodulin protein kinase II activity in the lumbar spinal cord after acute i.t. administration of nicotine was investigated in wild-type and β 2 and α 7 knockout mice, the increase in calcium/calmodulin-dependent calmodulin protein kinase II activity was not significant reduced in α 7 KO mice but was eliminated in the β 2 KO mice. (aspetjournals.org)
  • In addition, L-type calcium channel blockers nimodipine and verapamil but not the N -methyl- d -aspartate antagonist MK-801 (dizocilpine maleate) blocked the increase in the kinase activity induced by nicotine. (aspetjournals.org)
  • In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. (neuromics.com)
  • CaMKK2 isoform-7 (WT) and the pG539fs*4 mutant were expressed in COS7 cells and the pG539fs*4 mutant was noted to have significantly higher basal and Ca 2+ -CaM dependent kinase activity compared with WT isoform-7. (endocrine-abstracts.org)
  • We hypothesise that the increase in the Ca 2+ -CaM dependent kinase activity as a result of the mutation, to be increasing the insulin secretion, probably via the upregulated transcription of INS-1 . (endocrine-abstracts.org)
  • However, calmodulin antagonists had no effect on its kinase activity. (biomedcentral.com)
  • The SignaTECT® Protein Kinase Assay Systems provide a rapid, radioactivity-based method for detection of kinase activity. (mybio.ie)
  • The perforated and numbered membrane allows researchers to measure from 1 up to 96 kinase reactions and does not require as much "hands-on" manipulation as other methods used to measure kinase activity. (mybio.ie)
  • 1. Anastassiadis T, Deacon SW, Devarajan K, Ma H, Peterson JR. (2011) Comprehensive assay of kinase catalytic activity reveals features of kinase inhibitor selectivity. (guidetoimmunopharmacology.org)
  • 2013) A broad activity screen in support of a chemogenomic map for kinase signalling research and drug discovery. (guidetoimmunopharmacology.org)
  • To identify the molecular processes leading to these adaptations, we performed Cre/loxP-mediated genetic ablations of two key regulators of gene expression in response to activity, the Ca 2+ /calmodulin-dependent protein kinase IV (CaMKIV) and its postulated main target, the cAMP-responsive element binding protein (CREB). (pnas.org)
  • In this study, we found that thrombin increased Ca 2+ /calmodulin-dependent protein kinase II (CaM kinase II) activities in a Ca 2+ - and time-dependent manner in bovine pulmonary endothelium with maximal activity at 5 min. (elsevier.com)
  • Incubation of the kinase with either protease resulted in a three- to fivefold enhancement of total kinase activity and solubilization of Ca 2+ /calmodulin (CaM)-independent activity from the PSD. (elsevier.com)
  • Peptide kinase activity of fully dephosphorylated CPK28 was Ca 2+ -responsive and was inhibited by Ca 2+ /CaM. (illinois.edu)
  • Using in situ autophosphorylated protein, we expand on the known set of CPK28 autophosphorylation sites, and we demonstrate that, unexpectedly, autophosphorylated CPK28 had enhanced kinase activity at physiological concentrations of Ca 2+ compared with the dephosphorylated protein, suggesting that autophosphorylation functions to prime CPK28 for Ca 2+ activation and might also allow CPK28 to remain active when Ca 2+ levels are low. (illinois.edu)
  • CaMKI was phosphorylated on Thr177 and its activity enhanced approximately 25-fold by CaMKI kinase in a Ca(2+)-CaM dependent manner. (duke.edu)
  • Additionally, CaMKI kinase responds directly to Ca(2+)-CaM with increased activity. (duke.edu)
  • It did so without affecting the activity of known insulin signaling proteins. (edu.au)
  • Reducing intracellular calcium concentration inactivates MLCK but does not stop smooth muscle contraction since the myosin light chain has been physically modified through phosphorylation(and not via ATPase activity). (wikipedia.org)
  • Rho kinase also modulates the activity of MYLK by downregulating the activity of MYLK's counterpart protein: Myosin Light Chain Phosphatase (MYLP). (wikipedia.org)
  • The encoded protein is a MAGUK (membrane-associated guanylate kinase) protein family member. (genecards.org)
  • Analysis of the proteolytic products by Western blotting with a polyclonal antibody raised against soluble CaM-kinase II indicated that both proteases generated several immunoreactive fragments between 21 and 32 kDa. (elsevier.com)
  • Immunoblotting with antibody to the catalytic domain of the kinase indicated that there was a single active fragment of approximately 30 kDa in the μ-calpain digests. (elsevier.com)
  • Antibody microarray analysis identified 46 proteins that were differentially expressed between normal colorectal epithelium and adenocarcinoma. (mcponline.org)
  • To overcome this limitation and for a gain in sensitivity, we decided to test an antibody microarray strategy for detecting low abundance proteins. (mcponline.org)
  • In addition, the protein/antibody pairs are known " a priori . (mcponline.org)
  • The antibody microarray used in these studies contained 224 different antibodies ( 11 ), representing markers for eight biological pathways of interest (apoptosis, cell cycle, neurobiology, cytoskeleton, signal transduction, and nuclear proteins). (mcponline.org)
  • One of these is found in both the particulate and cytosolic fractions and phosphorylates a region of protein I that is phosphorylated in intact synaptosomes in response to calcium but not to cyclic AMP. (caltech.edu)
  • A second calcium-stimulated protein I kinase is found only in the cytosol and phosphorylates a region of protein I that is phosphorylated in intact synaptosomes in response to either calcium or cyclic AMP. (caltech.edu)
  • Myosin light-chain kinase also known as MYLK or MLCK is a serine/threonine-specific protein kinase that phosphorylates a specific myosin light chain, namely, the regulatory light chain of myosin II. (wikipedia.org)
  • it encodes a protein with a predicted molecular mass of 57,175 daltons. (sciencemag.org)
  • This gene encodes a protein similar to calcium/calmodulin dependent protein kinase, however, its exact function is not known. (wikipedia.org)
  • This gene encodes a protein that is a member of the dickkopf family. (antibodies-online.com)
  • This effect appeared to be mediated by phosphorylation of a synaptic vesicle protein, synapsin I, which in its dephosphorylated form inhibited synaptic transmission. (pnas.org)
  • This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. (rcsb.org)
  • Member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. (creative-biogene.com)
  • Two calcium-stimulated protein kinase activities (ATP:protein phosphotransferase, EC 2.7.1.37) that phosphorylate protein I, a specific synaptic protein, have been identified in homogenates of rat brain. (caltech.edu)
  • Multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. (genecards.org)
  • To assess the involvement of CaM kinase in experience-dependent behavior in an intact animal, we have designed a specific peptide inhibitor of CaM kinase and made transgenic Drosophila that express it under control of an inducible promoter. (nih.gov)
  • Kinetic analysis showed that the kinase not previously autophosphorylated had a K m for the synthetic peptide syntide-2 of 22.1 μ m and a V max of 644.1 n m min −1 mg −1 when assayed in the presence of Ca 2+ /CaM. (plantphysiol.org)
  • Contractile function was also preserved in colon ascendens stent peritonitis myocytes isolated from transgenic mice expressing a calcium and calmodulin-dependent protein kinase II inhibitory peptide (AC3-I) and in colon ascendens stent peritonitis myocytes isolated from mutant mice that have the ryanodine receptor 2 calcium and calmodulin-dependent protein kinase II-dependent phosphorylation site (serine 2814) mutated to alanine (S2814A). (ovid.com)
  • Furthermore, a cell-permeable N-myristoylated synthetic filamin peptide (containing the COOH-terminal CaM kinase II phosphorylation site) attenuated both thrombin-induced filamin phosphorylation and decreases in TER. (elsevier.com)
  • Our results demonstrate the importance of the serine/threonine kinase CaMK4 in the generation and function of regulatory T cells in patients with SLE and lupus-prone mice, and its potential to serve as a therapeutic target. (jimmunol.org)
  • Dieses CAMK4 Protein ist gelabelt mit His tag. (antikoerper-online.de)
  • Recombinant human CAMK4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. (antikoerper-online.de)
  • Calcium/calmodulin-dependent protein kinase IV (CaMK4) is expressed at increased levels in T cells from SLE patients (4) and lupus-prone mice (5). (bmj.com)
  • 2014) CaMK4-dependent activation of AKT/mTOR and CREM-α underlies autoimmunity-associated Th17 imbalance. (guidetoimmunopharmacology.org)
  • Calcium has a key role in mediating glucose stimulated insulin secretion via the action of multifunctional kinases (CaM-KI and CaM-KIV), activated by CaMKK2 , an upstream kinase. (endocrine-abstracts.org)
  • Expression analysis determined that each of the kinases was expressed during growth in vitro and also during infection. (edu.au)
  • Proteomic analysis of calcium/calmodulin-dependent protein kinase I and IV in vitro substrates reveals distinct catalytic preferences. (duke.edu)
  • Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. (creativebiomart.net)
  • These proteins are scaffold proteins and the encoded protein is located at synapses in the brain. (genecards.org)
  • Upon NMDA receptor-mediated Ca(2+) elevation, promotes dendritic growth in hippocampal neurons and is essential in synapses for full long-term potentiation (LTP) and ERK2-dependent translational activation. (abcam.com)
  • Human Ca(2+)-calmodulin (CaM) dependent protein kinase I (CaMKI) encodes a 370 amino acid protein with a calculated M(r) of 41,337. (duke.edu)
  • Whereas CaMKI 1-306 was unresponsive to CaMKI kinase, the 1-294 mutant was phosphorylated and activated by CaMKI kinase in both the presence and absence of Ca(2+)-CaM although at a faster rate in its presence. (duke.edu)
  • The NMDA receptor is ionotropic , meaning it is a protein which allows the passage of ions through the cell membrane. (wikipedia.org)
  • Wu, H-H & Lee, H-M 1999, ' Amphetamine activate protein kinase C and calcium/calmodulin-dependent protein kinase via NMDA receptor in primary cultures of rat cortical neurons ', New Taipei Journal of Medicine , vol. 1, pp. 12-19. (elsevier.com)
  • However, other molecular changes in other neurotransmitter receptors such as N-methyl-D-aspartate (NMDA) receptors and signaling molecules including protein kinases are proposed to be involved in morphine-induced analgesic tolerance ( 9 , 11 - 13 ). (kowsarpub.com)
  • Furthermore, our findings indicate that nicotinic stimulation of β 2 -containing acetylcholine nicotinic receptors in the spinal cord can activate calcium/calmodulin-dependent calmodulin protein kinase II and produce nicotinic analgesia, which may require L-type calcium voltage and gated channels but not the intervention of glutamatergic transmission. (aspetjournals.org)
  • Most of the known immunomodulatory effects of adenosine are mediated through its interaction with specific cell surface G protein-coupled receptors. (jimmunol.org)
  • Recent evidence suggests a central role for Ras in transmitting signals from G-protein coupled receptors, from growth factor receptors and from cytokine receptors not only down the Raf-MEK-ERK pathway to the nucleus, but also to various other cytosolic effectors. (biomedsearch.com)
  • Research suggests that the CASK protein may also interact with the protein produced from another gene, FRMD7 , to promote development of the nerves that control eye movement (the oculomotor neural network). (medlineplus.gov)
  • The mutations that cause CASK -related intellectual disability affect the role of the CASK protein in brain development and function. (medlineplus.gov)
  • MICPCH is caused by mutations that eliminate CASK function, while mutations that impair the function of this protein cause XL-ID with or without nystagmus. (medlineplus.gov)
  • Affected individuals with nystagmus may have CASK gene mutations that disrupt the interaction between the CASK protein and the protein produced from the FRMD7 gene, leading to problems with the development of the oculomotor neural network and resulting in abnormal eye movements. (medlineplus.gov)
  • Immunoblotting, coimmunoprecipitation, histological methods and behavioural assays (including home cage, open field, auditory and contextual fear conditioning and conditioned taste aversion) were applied to investigate expression of CASK and its related proteins, the protein-protein interactions of CASK, and anatomic and behavioural features of CASK T740A mice. (jpn.ca)
  • The functions of other CASK protein interactions cannot be addressed using CASK T740A mice. (jpn.ca)
  • This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag. (cusabio.com)
  • NtCaMK1 has all 11 subdomains of a kinase catalytic domain, lacks EF hands for Ca 2+ -binding, and is structurally similar to other CaMKs in mammal systems. (plantphysiol.org)
  • This protein contains a catalytic domain similar to that of calmodulin-dependent kinases and a calmodulin-like region with four calcium binding domains (EF hands). (sciencemag.org)
  • Thus, μ-calpain appears to cleave CaM-kinase 11 into a 30-kDa catalytic domain fragment and a 23-kDa regulatory domain fragment. (elsevier.com)
  • In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. (rcsb.org)
  • Calcium/calmodulin-dependent protein kinase type IV (CaMKIV) inhibits apoptosis induced by potassium deprivation in cerebellar granule neurons. (semanticscholar.org)
  • Nerve growth factor, brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT3), and NT4 belong to the neurotrophin family of signaling proteins essential for the survival and differentiation of different populations of neurons. (rupress.org)
  • We previously demonstrated that phosphorylation of the cyclic-AMP response element-binding protein (CREB) provides a readout of the instructive signals that guide plasticity in juveniles. (hindawi.com)
  • However, overactivation of NMDAR increases the cytosolic concentrations of calcium and zinc , which significantly contributes to neural death , that is found to be prevented by cannabinoids , with requirement of a HINT1 protein to counteract the toxic effects of NMDAR-mediated NO production and zinc release, through activation of the CB1 receptor . (wikipedia.org)
  • 125 I-labeled CaM overlays indicated a major CaM-binding fragment of approximately 23 kDa in μ-calpain digests of purified cytosolic CaM-kinase II. (elsevier.com)
  • Calcium/calmodulin-dependent protein kinase types II and IV differentially regulate CREB-dependent gene expression. (asm.org)
  • Dexamethasone (Dex) treatment increased mRNA and protein expression of cardiomyogenic markers cardiac troponin T and α-smooth muscle actin in CPCeδB compared with CPCe, suggesting increased differentiation. (escholarship.org)
  • The onset of sporulation triggered increased transcript levels of each of the kinases, particularly CpkA where an 11-fold increase in expression was observed during sporulation in planta. (edu.au)
  • These findings reveal a critical role for CaMKIV in the development and persistence of cocaine-induced behaviors, through mechanisms dissociated from acute effects on gene expression and CREB-dependent transcription. (pnas.org)
  • The cAMP-responsive element binding protein (CREB) and the related cAMP response element modulator (CREM) have been shown to be the main transcription factors regulating IEG expression ( 2 , 3 ). (pnas.org)
  • The expression of brain-derived neurotrophic factor and the α subunit of calcium/calmodulin-dependent protein kinase II mRNA in hippocampi obtained during surgical resections for intractable temporal lobe epilepsy were examined. (elsevier.com)
  • Several lines of evidence indicate NO as a key signaling molecule in mediating various plant responses such as photosynthesis, oxidative defense, osmolyte accumulation, gene expression, and protein modifications under heat stress. (frontiersin.org)
  • Previously we have carried out studies on differential protein expression analysis based on two-dimensional DIGE gels ( 10 ), which enabled us to identify the most abundant proteins in CRC tissues, including some isoforms and post-translational modifications. (mcponline.org)
  • Hence microarrays allow for a rapid identification of low expression proteins such as signaling molecules, cell cycle regulators, etc. (mcponline.org)
  • The absence of protein expression was confirmed by western blot analysis on brain extracts. (jax.org)
  • In addition, this systematic approach suggests a new subset of protein kinases including Nek9, which are differentially phosphorylated after short time (4-min) treatment of cells with the Met-activating InlB 321 . (mcponline.org)
  • Calmodulin trapping provides for molecular potentiation of calcium transients and may enable detection of their frequency. (sciencemag.org)
  • CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. (creative-biogene.com)
  • These kinases belong to a diverse group of enzymes that participate in many cellular responses and are activated by increasing concentrations of intracellular Ca 2+ . (diabetesjournals.org)
  • However, unlike chymotrypsin, μ-calpain degraded only a small fraction of the intact kinase subunits. (elsevier.com)
  • Cell surface binding of IFN-γ induces dimerization of its receptor subunits (IFNGR1 and IFNGR2) and subsequent activation of the receptor-associated JAK kinases 1 and 2 ( 9 ). (jimmunol.org)
  • CaMKK β is a major kinase activated by elevated levels of intracellular calcium. (ahajournals.org)
  • Traumatic brain injury (TBI) can dramatically increase levels of intracellular calcium ([Ca 2+ ] i ). (elsevier.com)
  • We selected most pathways CAMK2A participated on our site, such as ErbB signaling pathway, Calcium signaling pathway, cAMP signaling pathway, which may be useful for your reference. (creativebiomart.net)
  • Among its related pathways are Calcium signaling pathway and DAG and IP3 signaling . (genecards.org)
  • They are activated through multiple signal pathways, including by Ca 2+ /calmodulin-dependent kinase IV (CaMKIV) and also cAMP-dependent signaling and the MAPK pathway, although other signaling cascades have also been implicated ( 2 , 4 ). (pnas.org)
  • It is well-known that SNF1-related kinase 2s (SnRK2s) and pathogenesis-related (PRs) are major factors that influence plant drought tolerance by ABA- and SA-mediated pathways, respectively. (frontiersin.org)
  • Calcium and calmodulin-dependent protein kinase II, through phosphorylation of the ryanodine receptor would lead to Ca 2+ leak from the sarcoplasmic reticulum, reducing sarcoplasmic reticulum Ca 2+ content, Ca 2+ transient amplitude and contractility. (ovid.com)
  • The present study was undertaken to investigate the hypothesis that the β 2 nicotinic receptor subunit plays a central role in nicotine-induced spinal antinociception via calcium/calmodulin-dependent calmodulin protein kinase II activation. (aspetjournals.org)
  • Conclusions LS upregulates KCa2.3 and KCa3.1 expressions via CREB phosphorylation through activation of CaMKK and AMPK, a downstream kinase of CaMKK, whereas TNF- α suppresses both channel expressions. (ahajournals.org)
  • CREB, cAMP response element-binding protein. (aspetjournals.org)
  • Although the main role of CaMKIV might be activation of CREB, it is also reported to phosphorylate the CREB-binding protein ( 12 , 13 ) and to regulate histone deacetylase (HDAC) trafficking ( 14 , 15 ). (pnas.org)
  • The glideosome of T. gondii is a macromolecular complex that includes myosin A, myosin light chain (TgMLC1), T. gondii glideosome-associated protein 50 (TgGAP50), TgGAP45, aldolase 1, and actin 1 (TgACT1) [ 20 ]. (biomedcentral.com)
  • May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1. (genecards.org)
  • On the other side of the kinase at the N-Terminus end, sits the actin-binding domain, which allows MYLK to form interactions with actin filaments, keeping it in place. (wikipedia.org)
  • In addition to downregulation of MYLK, ROCK indirectly strengthens actin/myosin contraction through inhibiting Cofilin, a protein which depolymerizes actin stress fibers. (wikipedia.org)
  • Wnt signaling can also prompt morphological changes to cellular structure e.g., the non-canonical planar cell polarity pathway induces a kinase cascade that results in reorganization of actin, a core component of the cytoskeleton. (antibodies-online.com)
  • By fluorescence emission anisotropy, the affinity of CaM kinase for dansylated calmodulin was measured and found to increase 1000 times after autophosphorylation of the threonine at position 286 of the protein. (sciencemag.org)
  • The kinase itself binds calcium with high affinity. (sciencemag.org)
  • We therefore screened a subset of plant CPKs for CaM binding and found that CPK28 is a high affinity Ca 2+ /CaM-binding protein. (illinois.edu)
  • To identify the olomoucine-sensitive kinase(s), we prepared analogs that could be immobilized to an affinity resin to isolate binding proteins. (edu.au)
  • Binding of calcium ion to this domain increases the affinity of MYLK binding to myosin light chain. (wikipedia.org)
  • Since the CaM-K inhibitor blocked calcium stimulation of insulin gene transcription ( 10 ), this finding suggests the participation of CaM-Ks in glucose control of the gene. (diabetesjournals.org)
  • Calcium can function as a second messenger through stimulation of calcium-dependent protein kinases. (sciencemag.org)
  • Relative to autopsy control tissue, decreased hybridization to mRNA of the α subunit of calcium/calmodulin-dependent protein kinase II and increased hybridization to brain-derived neurotrophic factor mRNA were found throughout the granule cells of the epileptic hippocampus. (elsevier.com)
  • The analysis of regulated phosphorylation events on protein kinases is therefore of particular relevance, although this could not as yet be characterized systematically by proteomics. (mcponline.org)
  • A gene on chromosome 17p13.2 that encodes a calcium/calmodulin-dependent serine/threonine protein kinase, which mediates many of calcium's second messenger effects. (thefreedictionary.com)
  • The CAMK2 protein kinases contain a unique C-terminal subunit association domain responsible for oligomerization. (rcsb.org)
  • p>When browsing through different UniProt proteins, you can use the 'basket' to save them, so that you can back to find or analyse them later. (uniprot.org)
  • Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed. (uniprot.org)
  • The kinases were identified from the S. nodorum genome sequence on the basis of sequence homology to known Ca2+/calmodulin- dependent protein kinases. (edu.au)