Calcium-Calmodulin-Dependent Protein Kinase Type 2: A multifunctional calcium-calmodulin-dependent protein kinase subtype that occurs as an oligomeric protein comprised of twelve subunits. It differs from other enzyme subtypes in that it lacks a phosphorylatable activation domain that can respond to CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASE KINASE.Calcium-Calmodulin-Dependent Protein Kinases: A CALMODULIN-dependent enzyme that catalyzes the phosphorylation of proteins. This enzyme is also sometimes dependent on CALCIUM. A wide range of proteins can act as acceptor, including VIMENTIN; SYNAPSINS; GLYCOGEN SYNTHASE; MYOSIN LIGHT CHAINS; and the MICROTUBULE-ASSOCIATED PROTEINS. (From Enzyme Nomenclature, 1992, p277)Calmodulin: A heat-stable, low-molecular-weight activator protein found mainly in the brain and heart. The binding of calcium ions to this protein allows this protein to bind to cyclic nucleotide phosphodiesterases and to adenyl cyclase with subsequent activation. Thereby this protein modulates cyclic AMP and cyclic GMP levels.Cyclic GMP-Dependent Protein Kinase Type I: A cyclic GMP-dependent protein kinase subtype that is expressed in SMOOTH MUSCLE tissues and plays a role in regulation of smooth muscle contraction. Two isoforms, PKGIalpha and PKGIbeta, of the type I protein kinase exist due to alternative splicing of its mRNA.Cyclic GMP-Dependent Protein Kinase Type II: A cyclic GMP-dependent protein kinase subtype that is expressed predominantly in INTESTINES, BRAIN, and KIDNEY. The protein is myristoylated on its N-terminus which may play a role its membrane localization.Calcium-Calmodulin-Dependent Protein Kinase Type 4: A monomeric calcium-calmodulin-dependent protein kinase subtype that is primarily expressed in neuronal tissues; T-LYMPHOCYTES and TESTIS. The activity of this enzyme is regulated by its phosphorylation by CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASE KINASE.Protein Kinases: A family of enzymes that catalyze the conversion of ATP and a protein to ADP and a phosphoprotein.Phosphorylation: The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.Cyclic GMP-Dependent Protein Kinases: A group of cyclic GMP-dependent enzymes that catalyze the phosphorylation of SERINE or THREONINE residues of proteins.Calcium: A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.Cyclic AMP-Dependent Protein Kinase Type II: A cyclic AMP-dependent protein kinase subtype primarily found in particulate subcellular fractions. They are tetrameric proteins that contain two catalytic subunits and two type II-specific regulatory subunits.Protein Kinase C: An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters.Cyclic AMP-Dependent Protein Kinases: A group of enzymes that are dependent on CYCLIC AMP and catalyze the phosphorylation of SERINE or THREONINE residues on proteins. Included under this category are two cyclic-AMP-dependent protein kinase subtypes, each of which is defined by its subunit composition.Calcium-Calmodulin-Dependent Protein Kinase Type 1: A monomeric calcium-calmodulin-dependent protein kinase subtype that is expressed in a broad variety of mammalian cell types. Its expression is regulated by the action of CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASE KINASE. Several isoforms of this enzyme subtype are encoded by distinct genes.Benzylamines: Toluenes in which one hydrogen of the methyl group is substituted by an amino group. Permitted are any substituents on the benzene ring or the amino group.Enzyme Activation: Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.Cyclic AMP: An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Isoenzymes: Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.Calcium Signaling: Signal transduction mechanisms whereby calcium mobilization (from outside the cell or from intracellular storage pools) to the cytoplasm is triggered by external stimuli. Calcium signals are often seen to propagate as waves, oscillations, spikes, sparks, or puffs. The calcium acts as an intracellular messenger by activating calcium-responsive proteins.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Cyclic AMP-Dependent Protein Kinase RIalpha Subunit: A type I cAMP-dependent protein kinase regulatory subunit that plays a role in confering CYCLIC AMP activation of protein kinase activity. It has a lower affinity for cAMP than the CYCLIC-AMP-DEPENDENT PROTEIN KINASE RIBETA SUBUNIT.Kinetics: The rate dynamics in chemical or physical systems.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Protein Kinase Inhibitors: Agents that inhibit PROTEIN KINASES.Cyclic AMP-Dependent Protein Kinase Type I: A cyclic AMP-dependent protein kinase subtype primarily found in the CYTOPLASM. They are tetrameric proteins that contain two catalytic subunits and two type I-specific regulatory subunits.Cyclic GMP: Guanosine cyclic 3',5'-(hydrogen phosphate). A guanine nucleotide containing one phosphate group which is esterified to the sugar moiety in both the 3'- and 5'-positions. It is a cellular regulatory agent and has been described as a second messenger. Its levels increase in response to a variety of hormones, including acetylcholine, insulin, and oxytocin and it has been found to activate specific protein kinases. (From Merck Index, 11th ed)Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Protein-Serine-Threonine Kinases: A group of enzymes that catalyzes the phosphorylation of serine or threonine residues in proteins, with ATP or other nucleotides as phosphate donors.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Enzyme Inhibitors: Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Sporothrix: A mitosporic Ophiostomataceae fungal genus, whose species Sporothrix schenckii is a well-known animal pathogen. The conidia of this soil fungus may be inhaled causing a primary lung infection, or may infect independently via skin punctures.Calmodulin-Binding Proteins: Proteins which bind calmodulin. They are found in many tissues and have a variety of functions including F-actin cross-linking properties, inhibition of cyclic nucleotide phosphodiesterase and calcium and magnesium ATPases.Phosphotransferases (Alcohol Group Acceptor): A group of enzymes that transfers a phosphate group onto an alcohol group acceptor. EC 2.7.1.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Mitogen-Activated Protein Kinases: A superfamily of PROTEIN-SERINE-THREONINE KINASES that are activated by diverse stimuli via protein kinase cascades. They are the final components of the cascades, activated by phosphorylation by MITOGEN-ACTIVATED PROTEIN KINASE KINASES, which in turn are activated by mitogen-activated protein kinase kinase kinases (MAP KINASE KINASE KINASES).Paramecium tetraurelia: A species of ciliate protozoa. It is used in biomedical research.Sulfonamides: A group of compounds that contain the structure SO2NH2.Calcium-Binding Proteins: Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS.1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine: A specific protein kinase C inhibitor, which inhibits superoxide release from human neutrophils (PMN) stimulated with phorbol myristate acetate or synthetic diacylglycerol.Trifluoperazine: A phenothiazine with actions similar to CHLORPROMAZINE. It is used as an antipsychotic and an antiemetic.MAP Kinase Signaling System: An intracellular signaling system involving the MAP kinase cascades (three-membered protein kinase cascades). Various upstream activators, which act in response to extracellular stimuli, trigger the cascades by activating the first member of a cascade, MAP KINASE KINASE KINASES; (MAPKKKs). Activated MAPKKKs phosphorylate MITOGEN-ACTIVATED PROTEIN KINASE KINASES which in turn phosphorylate the MITOGEN-ACTIVATED PROTEIN KINASES; (MAPKs). The MAPKs then act on various downstream targets to affect gene expression. In mammals, there are several distinct MAP kinase pathways including the ERK (extracellular signal-regulated kinase) pathway, the SAPK/JNK (stress-activated protein kinase/c-jun kinase) pathway, and the p38 kinase pathway. There is some sharing of components among the pathways depending on which stimulus originates activation of the cascade.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Myosin-Light-Chain Kinase: An enzyme that phosphorylates myosin light chains in the presence of ATP to yield myosin-light chain phosphate and ADP, and requires calcium and CALMODULIN. The 20-kDa light chain is phosphorylated more rapidly than any other acceptor, but light chains from other myosins and myosin itself can act as acceptors. The enzyme plays a central role in the regulation of smooth muscle contraction.Phosphatidylinositol 3-Kinases: Phosphotransferases that catalyzes the conversion of 1-phosphatidylinositol to 1-phosphatidylinositol 3-phosphate. Many members of this enzyme class are involved in RECEPTOR MEDIATED SIGNAL TRANSDUCTION and regulation of vesicular transport with the cell. Phosphatidylinositol 3-Kinases have been classified both according to their substrate specificity and their mode of action within the cell.p38 Mitogen-Activated Protein Kinases: A mitogen-activated protein kinase subfamily that regulates a variety of cellular processes including CELL GROWTH PROCESSES; CELL DIFFERENTIATION; APOPTOSIS; and cellular responses to INFLAMMATION. The P38 MAP kinases are regulated by CYTOKINE RECEPTORS and can be activated in response to bacterial pathogens.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.eIF-2 Kinase: A dsRNA-activated cAMP-independent protein serine/threonine kinase that is induced by interferon. In the presence of dsRNA and ATP, the kinase autophosphorylates on several serine and threonine residues. The phosphorylated enzyme catalyzes the phosphorylation of the alpha subunit of EUKARYOTIC INITIATION FACTOR-2, leading to the inhibition of protein synthesis.Protein Structure, Tertiary: The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.Tetradecanoylphorbol Acetate: A phorbol ester found in CROTON OIL with very effective tumor promoting activity. It stimulates the synthesis of both DNA and RNA.Protein Kinase C-alpha: A cytoplasmic serine threonine kinase involved in regulating CELL DIFFERENTIATION and CELLULAR PROLIFERATION. Overexpression of this enzyme has been shown to promote PHOSPHORYLATION of BCL-2 PROTO-ONCOGENE PROTEINS and chemoresistance in human acute leukemia cells.Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.Pyruvate Kinase: ATP:pyruvate 2-O-phosphotransferase. A phosphotransferase that catalyzes reversibly the phosphorylation of pyruvate to phosphoenolpyruvate in the presence of ATP. It has four isozymes (L, R, M1, and M2). Deficiency of the enzyme results in hemolytic anemia. EC 2.7.1.40.Mitogen-Activated Protein Kinase 1: A proline-directed serine/threonine protein kinase which mediates signal transduction from the cell surface to the nucleus. Activation of the enzyme by phosphorylation leads to its translocation into the nucleus where it acts upon specific transcription factors. p40 MAPK and p41 MAPK are isoforms.Calcium Channels: Voltage-dependent cell membrane glycoproteins selectively permeable to calcium ions. They are categorized as L-, T-, N-, P-, Q-, and R-types based on the activation and inactivation kinetics, ion specificity, and sensitivity to drugs and toxins. The L- and T-types are present throughout the cardiovascular and central nervous systems and the N-, P-, Q-, & R-types are located in neuronal tissue.Death-Associated Protein Kinases: A family of calcium/calmodulin-dependent PROETIN-SERINE-THREONINE KINASES. They are ubiquitously expressed in adult and embryonic mammalian tissues, and their functions are tightly related to the early stages of eukaryotic programmed cell death.Cyclic AMP-Dependent Protein Kinase Catalytic Subunits: Specific enzyme subunits that form the active sites of the type I and type II cyclic-AMP protein kinases. Each molecule of enzyme contains two catalytic subunits.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.Rats, Sprague-Dawley: A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Mycelium: The body of a fungus which is made up of HYPHAE.Phosphoprotein Phosphatases: A group of enzymes removing the SERINE- or THREONINE-bound phosphate groups from a wide range of phosphoproteins, including a number of enzymes which have been phosphorylated under the action of a kinase. (Enzyme Nomenclature, 1992)Mitogen-Activated Protein Kinase Kinases: A serine-threonine protein kinase family whose members are components in protein kinase cascades activated by diverse stimuli. These MAPK kinases phosphorylate MITOGEN-ACTIVATED PROTEIN KINASES and are themselves phosphorylated by MAP KINASE KINASE KINASES. JNK kinases (also known as SAPK kinases) are a subfamily.Mitogen-Activated Protein Kinase 3: A 44-kDa extracellular signal-regulated MAP kinase that may play a role the initiation and regulation of MEIOSIS; MITOSIS; and postmitotic functions in differentiated cells. It phosphorylates a number of TRANSCRIPTION FACTORS; and MICROTUBULE-ASSOCIATED PROTEINS.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Protein Kinase C-delta: A ubiquitously expressed protein kinase that is involved in a variety of cellular SIGNAL PATHWAYS. Its activity is regulated by a variety of signaling protein tyrosine kinase.Isoquinolines: A group of compounds with the heterocyclic ring structure of benzo(c)pyridine. The ring structure is characteristic of the group of opium alkaloids such as papaverine. (From Stedman, 25th ed)Adenosine Triphosphate: An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.JNK Mitogen-Activated Protein Kinases: A subgroup of mitogen-activated protein kinases that activate TRANSCRIPTION FACTOR AP-1 via the phosphorylation of C-JUN PROTEINS. They are components of intracellular signaling pathways that regulate CELL PROLIFERATION; APOPTOSIS; and CELL DIFFERENTIATION.Peptide T: N-(N-(N(2)-(N-(N-(N-(N-D-Alanyl L-seryl)-L-threonyl)-L-threonyl) L-threonyl)-L-asparaginyl)-L-tyrosyl) L-threonine. Octapeptide sharing sequence homology with HIV envelope protein gp120. It is potentially useful as antiviral agent in AIDS therapy. The core pentapeptide sequence, TTNYT, consisting of amino acids 4-8 in peptide T, is the HIV envelope sequence required for attachment to the CD4 receptor.Serine: A non-essential amino acid occurring in natural form as the L-isomer. It is synthesized from GLYCINE or THREONINE. It is involved in the biosynthesis of PURINES; PYRIMIDINES; and other amino acids.PhosphoproteinsNucleotides, CyclicEgtazic Acid: A chelating agent relatively more specific for calcium and less toxic than EDETIC ACID.Molecular Weight: The sum of the weight of all the atoms in a molecule.AMP-Activated Protein Kinases: Intracellular signaling protein kinases that play a signaling role in the regulation of cellular energy metabolism. Their activity largely depends upon the concentration of cellular AMP which is increased under conditions of low energy or metabolic stress. AMP-activated protein kinases modify enzymes involved in LIPID METABOLISM, which in turn provide substrates needed to convert AMP into ATP.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Rabbits: The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.Neurons: The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.Protamine Kinase: An aspect of protein kinase (EC 2.7.1.37) in which serine residues in protamines and histones are phosphorylated in the presence of ATP.Cytosol: Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.Recombinant Fusion Proteins: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.Dose-Response Relationship, Drug: The relationship between the dose of an administered drug and the response of the organism to the drug.Calcium, Dietary: Calcium compounds used as food supplements or in food to supply the body with calcium. Dietary calcium is needed during growth for bone development and for maintenance of skeletal integrity later in life to prevent osteoporosis.src-Family Kinases: A PROTEIN-TYROSINE KINASE family that was originally identified by homology to the Rous sarcoma virus ONCOGENE PROTEIN PP60(V-SRC). They interact with a variety of cell-surface receptors and participate in intracellular signal transduction pathways. Oncogenic forms of src-family kinases can occur through altered regulation or expression of the endogenous protein and by virally encoded src (v-src) genes.Hippocampus: A curved elevation of GRAY MATTER extending the entire length of the floor of the TEMPORAL HORN of the LATERAL VENTRICLE (see also TEMPORAL LOBE). The hippocampus proper, subiculum, and DENTATE GYRUS constitute the hippocampal formation. Sometimes authors include the ENTORHINAL CORTEX in the hippocampal formation.Protein Kinase C-epsilon: A protein kinase C subtype that was originally characterized as a CALCIUM-independent, serine-threonine kinase that is activated by PHORBOL ESTERS and DIACYLGLYCEROLS. It is targeted to specific cellular compartments in response to extracellular signals that activate G-PROTEIN-COUPLED RECEPTORS; TYROSINE KINASE RECEPTORS; and intracellular protein tyrosine kinase.Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Protein Kinase C beta: PKC beta encodes two proteins (PKCB1 and PKCBII) generated by alternative splicing of C-terminal exons. It is widely distributed with wide-ranging roles in processes such as B-cell receptor regulation, oxidative stress-induced apoptosis, androgen receptor-dependent transcriptional regulation, insulin signaling, and endothelial cell proliferation.Protein-Tyrosine Kinases: Protein kinases that catalyze the PHOSPHORYLATION of TYROSINE residues in proteins with ATP or other nucleotides as phosphate donors.Models, Biological: Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.Time Factors: Elements of limited time intervals, contributing to particular results or situations.1-Phosphatidylinositol 4-Kinase: An enzyme that catalyzes the conversion of phosphatidylinositol (PHOSPHATIDYLINOSITOLS) to phosphatidylinositol 4-phosphate, the first committed step in the biosynthesis of phosphatidylinositol 4,5-bisphosphate.

Differential effects of a calcineurin inhibitor on glutamate-induced phosphorylation of Ca2+/calmodulin-dependent protein kinases in cultured rat hippocampal neurons. (1/181)

Calcium/calmodulin-dependent protein kinases (CaM kinases) are major multifunctional enzymes that play important roles in calcium-mediated signal transduction. To characterize their regulatory mechanisms in neurons, we compared glutamate-induced phosphorylation of CaM kinase IV and CaM kinase II in cultured rat hippocampal neurons. We observed that dephosphorylation of these kinases followed different time courses, suggesting different regulatory mechanisms for each kinase. Okadaic acid, an inhibitor of protein phosphatase (PP) 1 and PP2A, increased the phosphorylation of both kinases. In contrast, cyclosporin A, an inhibitor of calcineurin, showed different effects: the phosphorylation and activity of CaM kinase IV were significantly increased with this inhibitor, but those of CaM kinase II were not significantly increased. Cyclosporin A treatment of neurons increased phosphorylation of Thr196 of CaM kinase IV, the activated form with CaM kinase kinase, which was recognized with an anti-phospho-Thr196 antibody. Moreover, recombinant CaM kinase IV was dephosphorylated and inactivated with calcineurin as well as with PP1, PP2A, and PP2C in vitro. These results suggest that CaM kinase IV, but not CaM kinase II, is directly regulated with calcineurin.  (+info)

Inhibition of the Ca2+/calmodulin-dependent protein kinase I cascade by cAMP-dependent protein kinase. (2/181)

Several recent studies have shown that Ca2+/calmodulin-dependent protein kinase I (CaMKI) is phosphorylated and activated by a protein kinase (CaMKK) that is itself subject to regulation by Ca2+/calmodulin. In the present study, we demonstrate that this enzyme cascade is regulated by cAMP-mediated activation of cAMP-dependent protein kinase (PKA). In vitro, CaMKK is phosphorylated by PKA and this is associated with inhibition of enzyme activity. The major site of phosphorylation is threonine 108, although additional sites are phosphorylated with lower efficiency. In vitro, CaMKK is also phosphorylated by CaMKI at the same sites as PKA, suggesting that this regulatory phosphorylation might play a role as a negative-feedback mechanism. In intact PC12 cells, activation of PKA with forskolin resulted in a rapid inhibition of both CaMKK and CaMKI activity. In hippocampal slices CaMKK was phosphorylated under basal conditions, and activation of PKA led to an increase in phosphorylation. Two-dimensional phosphopeptide mapping indicated that activation of PKA led to increased phosphorylation of multiple sites including threonine 108. These results indicate that in vitro and in intact cells the CaMKK/CaMKI cascade is subject to inhibition by PKA-mediated phosphorylation of CaMKK. The phosphorylation and inhibition of CaMKK by PKA is likely to be involved in modulating the balance between cAMP- and Ca2+-dependent signal transduction pathways.  (+info)

Substrate recognition by Ca2+/Calmodulin-dependent protein kinase kinase. Role of the arg-pro-rich insert domain. (3/181)

Mammalian Ca2+/CaM-dependent protein kinase kinase (CaM-KK) has been identified and cloned as an activator for two kinases, CaM kinase I (CaM-KI) and CaM kinase IV (CaM-KIV), and a recent report (Yano, S., Tokumitsu, H., and Soderling, T. R. (1998) Nature 396, 584-587) demonstrates that CaM-KK can also activate and phosphorylate protein kinase B (PKB). In this study, we identify a CaM-KK from Caenorhabditis elegans, and comparison of its sequence with the mammalian CaM-KK alpha and beta shows a unique Arg-Pro (RP)-rich insert in their catalytic domains relative to other protein kinases. Deletion of the RP-domain resulted in complete loss of CaM-KIV activation activity and physical interaction of CaM-KK with glutathione S-transferase-CaM-KIV (T196A). However, CaM-KK autophosphorylation and phosphorylation of a synthetic peptide substrate were normal in the RP-domain mutant. Site-directed mutagenesis of three conserved Arg in the RP- domain of CaM-KK confirmed that these positive charges are important for CaM-KIV activation. The RP- domain deletion mutant also failed to fully activate and phosphorylate CaM-KI, but this mutant was indistinguishable from wild-type CaM-KK for the phosphorylation and activation of PKB. These results indicate that the RP-domain in CaM-KK is critical for recognition of downstream CaM-kinases but not for its catalytic activity (i.e. autophosphorylation) and PKB activation.  (+info)

The retinoblastoma susceptibility gene product/Sp1 signalling pathway is modulated by Ca2+/calmodulin kinases II and IV activity. (4/181)

To investigate the possible link between Ca2+ signalling and cell cycle control we analysed Ca2+/calmodulin kinases (CamK) interaction with the retinoblastoma susceptibility gene product/SP1 pathway. CamK II and IV activate c-fos transcription through a short promoter region (-99 to -53) containing the retinoblastoma control element (RCE) and a cAMP response element (CRE) related sequences. Deletion analysis revealed that the RCE is a major CamK responsive element and is sufficient to confer CamK and Ca2+ regulation to a minimal promoter. Direct interactions between SP1 and RCE were confirmed by gel shift experiments. Using transient transfection experiments, we show that CamK-dependent transcription is regulated by the retinoblastoma (Rb) susceptibility gene product and the p107 Rb related protein. However, the stimulatory effects of CamKs and Rb on c-fos are blocked by overexpression of both proteins. These effects appear to be directly mediated by SP1 as shown by the use of a Gal4/SP1 fusion proteins. In conclusion, CamK II and IV, two major Ca2+-dependent intracellular effectors, may represent a molecular link between this second messenger transduction pathway and effectors that control cell cycle progression through Rb/SP1 signalling pathway.  (+info)

A late phase of cerebellar long-term depression requires activation of CaMKIV and CREB. (5/181)

Recently, it has been shown that cerebellar LTD has a late phase that may be blocked by protein synthesis inhibitors. To understand the mechanisms underlying the late phase, we interfered with the activation of transcription factors that might couple synaptic activation to protein synthesis. Particle-mediated transfection of cultured Purkinje neurons with an expression vector encoding a dominant inhibitory form of CREB resulted in a nearly complete blockade of the late phase. Kinases that activate CREB were inhibited, and LTD was assessed. Inhibition of PKA or the MAPK/RSK cascades were without effect on the late phase, while constructs designed to interfere with CaMKIV function attenuated the late phase. These results indicate that the activation of CaMKIV and CREB are necessary to establish a late phase of cerebellar LTD.  (+info)

Ca(2+)-dependent gene expression mediated by MEF2 transcription factors. (6/181)

Ca(2+) induction of a subset of cellular and viral immediate-early activation genes in lymphocytes has been previously mapped to response elements recognized by the MEF2 family of transcription factors. Here, we demonstrate that Ca(2+) activation of MEF2 response elements in T lymphocytes is mediated in synergy by two Ca(2+)/calmodulin-dependent enzymes, the phosphatase calcineurin, and the kinase type IV/Gr (CaMKIV/Gr), which promote transcription by the MEF2 family members MEF2A and MEF2D. Calcineurin up-regulates the activity of both factors by an NFAT-dependent mechanism, while CaMKIV/Gr selectively and independently activates MEF2D. These results identify MEF2 proteins as effectors of a pathway of gene induction in T lymphocytes which integrates diverse Ca(2+) activation signals and may be broadly operative in several tissues.  (+info)

Activation of orphan receptor-mediated transcription by Ca(2+)/calmodulin-dependent protein kinase IV. (7/181)

Retinoid-related receptor alpha (RORalpha) is an orphan nuclear receptor that constitutively activates transcription from its cognate response element. We show that RORalpha is Ca(2+ )responsive, and a Ca(2+)/calmodulin-independent form of Ca(2+)/calmodulin-dependent protein kinase IV (CaMKIV) potentiates RORalpha-dependent transcription 20- to 30-fold. Other orphan receptors including RORalpha2, RORgamma and COUP-TFI are also potentiated by CaMKIV. Transcriptional activation by CaMKIV is orphan receptor selective and does not occur with either the thyroid hormone or estrogen receptor. CaMKIV does not phosphorylate RORalpha or its ligand-binding domain (LBD) in vitro, although the LBD is essential for transactivation. Therefore, the RORalpha LBD was used in the mammalian two-hybrid assay to identify a single class of small peptide molecules containing LXXLL motifs that interacted with greater affinity in the presence of CaMKIV. This class of peptides antagonized activation of orphan receptor-mediated transcription by CaMKIV. These studies demonstrate a pivotal role for CaMKIV in the regulation of orphan receptor-mediated transcription.  (+info)

Ca(2+)/calmodulin-dependent protein kinase IV is expressed in spermatids and targeted to chromatin and the nuclear matrix. (8/181)

Ca(2+)/calmodulin-dependent protein kinase IV and calspermin are two proteins encoded by the Camk4 gene. Both are highly expressed in the testis, where in situ hybridization studies in rat testes have demonstrated that CaMKIV mRNA is localized to pachytene spermatocytes, while calspermin mRNA is restricted to spermatids. We have examined the expression patterns of both CaMKIV and calspermin in mouse testis and unexpectedly find that CaMKIV is expressed in spermatogonia and spermatids but excluded from spermatocytes, while calspermin is found only in spermatids. CaMKIV and calspermin expression in the testis are stage-dependent and appear to be coordinately regulated. In germ cells, we find that CaMKIV is associated with the chromatin. We further demonstrate that a fraction of CaMKIV in spermatids is hyperphosphorylated and specifically localized to the nuclear matrix. These novel findings may implicate CaMKIV in chromatin remodeling during nuclear condensation of spermatids.  (+info)

*CAMK4

Calcium/calmodulin-dependent protein kinase type IV is an enzyme that in humans is encoded by the CAMK4 gene. The product of ... calmodulin-dependent protein kinase I by calmodulin and by Ca2+/calmodulin-dependent protein kinase kinase". J. Biol. Chem. 273 ... Phosphorylation by cyclic AMP-dependent protein kinase, protein kinase C, and calcium/calmodulin protein kinase; identification ... and activation of Ca2+-calmodulin-dependent protein kinase IV by Ca2+-calmodulin-dependent protein kinase Ia kinase. ...

*CASK

This gene is also known by several other names: CMG 2 (CAMGUK protein 2), calcium/calmodulin-dependent serine protein kinase 3 ... Hsueh YP, Roberts AM, Volta M, Sheng M, Roberts RG (June 2001). "Bipartite interaction between neurofibromatosis type I protein ... "Entrez Gene: CASK Calcium/calmodulin-dependent serine protein kinase (MAGUK family)". Tarpey PS, Smith R, Pleasance E, Whibley ... Zhu ZQ, Wang D, Xiang D, Yuan YX, Wang Y (2014). "Calcium/calmodulin-dependent serine protein kinase is involved in exendin-4- ...

*ATP2B1

Plasma membrane calcium-transporting ATPase 1 is an enzyme that in humans is encoded by the ATP2B1 gene. The protein encoded by ... 1991). "Protein kinase C phosphorylates the carboxyl terminus of the plasma membrane Ca(2+)-ATPase from human erythrocytes". J ... 1989). "Primary structure of the cAMP-dependent phosphorylation site of the plasma membrane calcium pump". Biochemistry. 28 (10 ... 1992). "Study of calmodulin binding to the alternatively spliced C-terminal domain of the plasma membrane Ca2+ pump". ...

*CDK5R2

... p39 interact with the alpha-subunit of Ca2+/calmodulin-dependent protein kinase II and alpha-actinin-1 in a calcium-dependent ... 2004). "Regulation of type 1 protein phosphatase/inhibitor-2 complex by glycogen synthase kinase-3beta in intact cells". J. ... p39 interact with the alpha-subunit of Ca2+/calmodulin-dependent protein kinase II and alpha-actinin-1 in a calcium-dependent ... Cyclin-dependent kinase 5 activator 2 is an enzyme that in humans is encoded by the CDK5R2 gene. The protein encoded by this ...

*CAPN1

Harris AS, Croall DE, Morrow JS (1988). "The calmodulin-binding site in alpha-fodrin is near the calcium-dependent protease-I ... "Primary structure of a gamma subunit of G protein, gamma 12, and its phosphorylation by protein kinase C". J. Biol. Chem. 270 ( ... The European Consortium on Men1, (GENEM 1; Groupe d'Etude des Néoplasies Endocriniennes Multiples de type 1)". Genomics. 37 (3 ... Sorimachi H, Ohmi S, Emori Y, Kawasaki H, Saido TC, Ohno S, Minami Y, Suzuki K (1990). "A novel member of the calcium-dependent ...

*Ca2+/calmodulin-dependent protein kinase II

Calcium-Calmodulin Dependent Protein Kinases at the US National Library of Medicine Medical Subject Headings (MeSH) To learn ... Erondu, N.E., and Kennedy, M.B. (1985). Regional distribution of type II Ca2+/calmodulin-dependent protein kinase in rat brain ... Ca2+ /calmodulin-dependent protein kinase II (CaM kinase II or CaMKII) is a serine/threonine-specific protein kinase that is ... Calcium/ calmodulin dependent protein kinase II is also heavily implicated in long-term potentiation (LTP) - the molecular ...

*Telokin

"Structure and expression of a calcium-binding protein gene contained within a calmodulin-regulated protein kinase gene". ... encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. Four transcript variants that produce four ... Reaction type: phospho group transfer) It requires Ca2+ and calmodulin for activity. The 20-kDa light chain from smooth muscle ... "Kinase-related protein (telokin) is phosphorylated by smooth-muscle myosin light-chain kinase and modulates the kinase activity ...

*Neuronal calcium sensor-1

PIK1 It binds to many proteins, some in calcium dependent and some in calcium independent ways, and switches many of the ... G-protein-coupled receptor kinase 2) D2 dopamine receptor IL1RAPL1 (interleukin-1 receptor accessory protein-like 1 protein) ... calcium-binding protein. Frq can substitute for calmodulin in some situations. It is thought to be associated with neuronal ... The designation 'NCS-1' came from the assumption that the protein was expressed only in neuronal cell types, which is not the ...

*CAMK2B

Calcium/calmodulin-dependent protein kinase type II beta chain is an enzyme that in humans is encoded by the CAMK2B gene. The ... CAMK2B calcium/calmodulin-dependent protein kinase (CaM kinase) II beta". Walikonis RS, Oguni A, Khorosheva EM, Jeng CJ, ... Novak G, Seeman P, Tallerico T (2001). "Schizophrenia: elevated mRNA for calcium-calmodulin-dependent protein kinase IIbeta in ... calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic ...

*S100B

... the microtubule-associated tau proteins and S100b regulate tau phosphorylation by the Ca2+/calmodulin-dependent protein kinase ... "The calcium-modulated proteins, S100A1 and S100B, as potential regulators of the dynamics of type III intermediate filaments". ... S100 calcium-binding protein B (S100B) is a protein of the S-100 protein family. S100 proteins are localized in the cytoplasm ... the microtubule-associated tau proteins and S100b regulate tau phosphorylation by the Ca2+/calmodulin-dependent protein kinase ...

*Actinin alpha 2

Zhang S, Ehlers MD, Bernhardt JP, Su CT, Huganir RL (August 1998). "Calmodulin mediates calcium-dependent inactivation of N- ... a striated muscle-restricted PDZ and LIM domain-containing protein, binds to alpha-actinin-2 and protein kinase C". The Journal ... Young P, Ferguson C, Bañuelos S, Gautel M (March 1998). "Molecular structure of the sarcomeric Z-disk: two types of titin ... ". "Protein Information - Basic Information: Protein COPaKB ID: P35609". Cardiac Organellar Protein Atlas Knowledgebase. Zong ...

*Calcium-binding protein 1

... a novel two EF-hand calcium-binding protein that suppresses Ca2+/calmodulin-dependent protein kinase II activity in the brain ... Expression of CaBP1 helps to block Ca2+-dependent facilitation of P/Q-type Ca2+ current which is markedly reduced facilitation ... Calcium binding protein 1 is a protein that in humans is encoded by the CABP1 gene. Calcium-binding protein 1 is a calcium- ... Calcium-binding protein 1 which is a neuron -specific member of the calmodulin (CaM) superfamily which modulates Ca2+-dependent ...

*ANK2

... suggesting that inhibition of Ca2+/calmodulin-dependent protein kinase II may also be a potential treatment strategy. These ... which can be explained by decreased voltage-dependent calcium channel expression, specifically Ca(v)1.3, which is responsible ... Initially, a Glu1425Gly mutation in ANK2 was found to cause dominantly-inherited long QT syndrome, type 4, cardiac arrhythmia. ... Effects on ryanodine receptors specifically were also rescued by a potent Ca2+/calmodulin-dependent protein kinase II inhibitor ...

*Frequency (gene)

... and a calcium/calmodulin-dependent kinase (CAMK-1), and additional kinases, reaching its peak around mid-subjective day. Kinase ... with just one of the proteins present. The choice of which protein is made is the result of temperature-dependent splicing of ... Because sFRQ favors a longer period than lFRQ, free running rhythms in wild type Neurospora are somewhat decreased with ... A similar palette of kinases modifies the clock proteins in all cases. White Collar-1 Colin Pittendrigh Period (gene) Casein ...

*Protein methylation

... other cellular proteins carry N-methyllysine residues including elongation factor 1A and the calcium sensing protein calmodulin ... The arginine methylation of proteins can either inhibit or promote protein-protein interactions depending on the type of ... GTP-binding proteins, nuclear lamins and certain protein kinases. Many of these proteins participate in cell signaling, and ... A well-characterized example of a methylation dependent protein-protein interaction is related to the selective methylation of ...

*CAMK2A

Calcium/calmodulin-dependent protein kinase type II alpha chain (CAMKIIα) is an enzyme that in humans is encoded by the CAMK2A ... Phosphorylation by cyclic AMP-dependent protein kinase, protein kinase C, and calcium/calmodulin protein kinase; identification ... "Entrez Gene: CAMK2A calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha". Walikonis RS, Oguni A, Khorosheva EM, ... calmodulin-dependent protein kinase II phosphorylates various types of non-epithelial intermediate filament proteins". Biochem ...

*PDE1

... (phosphodiesterase type 1) is a phosphodiesterase enzyme also known as calcium- and calmodulin-dependent phosphodiesterase ... of PDE1A1 and PDE1A2 by protein kinase A and of PDE1B1 by CaM Kinase II decreases their sensitivity to calmodulin activation. ... and calmodulin (CaM). Calmodulin has been shown to activate cyclic nucleotide PDE in a calcium-dependent manner and the ... basal and calmodulin stimulated purified PDE1. Since nimodipine at lower concentrations blocks the L-type calcium channel, it ...

*Alcino J. Silva

"Feasibility of long-term storage of graded information by the Ca2+/calmodulin-dependent protein kinase molecules of the ... Silva decided to study hippocampal-dependent memory formation. The compelling properties of calcium calmodulin kinase II, one ... Gutmann, DH; Parada, LF; Silva, AJ; Ratner, N (2012). "Neurofibromatosis type 1: modeling CNS dysfunction". J Neurosci. 32 (41 ... Silva, A.J.; Paylor, R.; Wehner, J.M.; Tonegawa, S. (1992). "Impaired spatial learning in alpha-calcium-calmodulin kinase II ...

*Ceramide kinase

"Calmodulin is involved in the Ca2+-dependent activation of ceramide kinase as a calcium sensor". J. Biol. Chem. 280 (49): 40436 ... CERK shares sequence homology with sphingosine kinase type I, including an N-terminal pleckstrin homology (PH) domain and a ... It was demonstrated that C-1-P perpetuates the phosphorylation of glycogen synthase kinase-3 β and retinoblastoma protein, ... CERK was later confirmed to bind calmodulin in the presence of calcium, indicating the calmodulin first binds calcium and then ...

*Ligand-gated ion channel

... which in turn go on to bind and allosterically activate enzymes such as Ca2+/calmodulin-dependent kinases (CAMKs). The ... and all are directly stimulated by G-proteins of the Gαs class. Interaction with Gα subunits of the Gαi/o type, on the contrary ... Action potential Voltage-dependent calcium channel Calcium-activated potassium channel Cyclic nucleotide-gated ion channel Acid ... Once bound to GTP, Rho can then go on to activate various proteins responsible for cytoskeleton regulation such as Rho-kinase ( ...

*CAMK2G

Calcium/calmodulin-dependent protein kinase type II gamma chain is an enzyme that in humans is encoded by the CAMK2G gene. The ... "Entrez Gene: CAMK2G calcium/calmodulin-dependent protein kinase (CaM kinase) II gamma". Moyers JS, Bilan PJ, Zhu J, Kahn CR ( ... calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic ... "Phosphorylation and inhibition of type III adenylyl cyclase by calmodulin-dependent protein kinase II in vivo". J. Biol. Chem. ...

*CACNA1H

2003). "A mechanism for the direct regulation of T-type calcium channels by Ca2+/calmodulin-dependent kinase II". J. Neurosci. ... a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell ... Calcium channel, voltage-dependent, T type, alpha 1H subunit, also known as CACNA1H, is a protein which in humans is encoded by ... 2003). "T-type calcium channel regulation by specific G-protein betagamma subunits". Nature. 424 (6945): 209-13. doi:10.1038/ ...

*Collapsin response mediator protein family

Ca2+/calmodulin-dependent protein kinase II (CaMK II) is also activated by calcium influx through NMDA receptors, and is ... Lacosamide is used in combination with other types of medications to control various types of seizures, especially epilepsy. ... In postsynaptic densities (PSD), CRMP-2 is phosphorylated by Ca2+/calmodulin-dependent protein kinase II (CaM kinase II), which ... and cyclin-dependent protein kinase 5 (Cdk5) are highly expressed in Alzheimer's disease and are some of the protein kinases ...

*Phosphorylase kinase

... but phosphorylation of both these subunits by protein kinase A (PKA, or cAMP-dependent protein kinase) reduces their respective ... Defects in phosphorylase kinase genes are the cause of glycogen storage disease type IX (GSD type IX) and GSD type VI (formerly ... activated when the β and α subunits are phosphorylated by protein kinase A and the delta subunit has bound to calcium ions. In ... The δ subunit is the ubiquitous eukaryotic protein calmodulin which itself has 4 calcium ion binding sites. When cytosolic Ca2+ ...

*Cav1.2

Calcium channel, voltage-dependent, L type, alpha 1C subunit (also known as Cav1.2) is a protein that in humans is encoded by ... When depolarization occurs, calcium ions flow through the channel and some bind to Calmodulin. The Calcium/Calmodulin binding ... Perets T, Blumenstein Y, Shistik E, Lotan I, Dascal N (Apr 1996). "A potential site of functional modulation by protein kinase ... This gene encodes an alpha-1 subunit of a voltage-dependent calcium channel. Calcium channels mediate the influx of calcium ...
Recombinant Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4) Protein (His tag). Spezies: Human. Quelle: Escherichia coli (E. coli). Jetzt Produkt ABIN668012 bestellen.
Results We show that culture of MRL/lpr Foxp3-GFP T cells in the presence of KN-93 promotes Treg differentiation in a dose dependent manner (Fig. F). Treatment of MRL/lpr Foxp3-GFP mice with KN-93 results in significant induction of Treg cells in the spleen, peripheral lymph nodes (Fig. B-E) and peripheral blood (Fig. A and B) and this is accompanied by decreased skin and kidney damage. Notably, KN-93 clearly diminishes the accumulation of inflammatory cells along with reciprocally increased Treg cells in target organ.. ...
Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates CREB1 and SYN1/synapsin I. Phosphorylates and activates CAMK1 (By similarity).
CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. Phosphorylates transcription factor FOXO3 on Ser-298 (By similarity). Activates FOXO3 transcriptional activity (PubMed:23805378).
Our previous studies demonstrated that subcutaneous injection of bee venom (BV) into the Zusanli (ST36) acupuncture point, namely BV acupuncture, dose-dependently prevents conditioned place preference (CPP) induced by repeated injection of methamphetamine (METH) in mice. To expand on our observations, the present study was designed to determine the suppressive mechanisms of BV acupuncture in the development of METH-induced CPP by evaluating the changes in expression of ΔFosB, phosphorylated extracellular signal-regulated kinase 1/2 (pERK), and phosphorylated calcium/calmodulin-dependent protein kinase type II (pCaMKII) in the prefrontal cortex (PFC) and nucleus accumbens (NAc) in mice. Pre-emptive treatment with BV at 30 min before repeated METH injection completely suppressed acquisition of CPP at the day 7 test session. METH-induced upregulation of ΔFosB and pERK in PFC and NAc was significantly reduced by BV pretreatment. Expression of pCaMKII was significantly elevated by METH in NAc and ...
human CaM Kinase 4/CaMKIV gene cDNA, cloning vector & expression plasmid, mutiple tags. Optimized for high expression in mammalian cells. Save up to 60%.
Buy our Recombinant Human CAMKIV protein. Ab60756 is a full length protein produced in Baculovirus infected Sf9 cells and has been validated in FuncS…
Looking for online definition of CaM-kinase kinase 1 in the Medical Dictionary? CaM-kinase kinase 1 explanation free. What is CaM-kinase kinase 1? Meaning of CaM-kinase kinase 1 medical term. What does CaM-kinase kinase 1 mean?
Complete information for CAMK4 gene (Protein Coding), Calcium/Calmodulin Dependent Protein Kinase IV, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
calmodulin-dependent protein kinase V: widely distributed in various tissues, involved in calcium-regulated processes; from rat brain; may exist in 40 & 41 kDa isoforms; amino acid sequence has been determined
In the present study, we have examined the role of the CaM-KK/CaM-KIV cascade on insulin gene expression in response to glucose. CaM-Ks are implicated in the regulation of a wide variety of biological events requiring the participation of intracellular Ca2+, such as muscle contraction, neurotransmitter release, and gene expression (11,26). Recent studies (19,26) have indicated that so-called multifunctional CaM-Ks, including CaM-KI, -II, and -IV, are regulated by phosphorylation either by itself or an upstream protein kinase, CaM-KK.. Previous reports (12) showed that CaM-KIV was expressed in pancreatic islet and other insulin-secreting cells, including MIN6 and HIT cells. In the first set of studies, we established that the respective mRNAs encoding CaM-KIV and its upstream protein kinase, CaM-KK, were present in rat pancreatic islet and the insulin-secreting cell line, INS-1. Furthermore, the mRNA was expressed because both Western blot and immunohistochemistry of the cells showed the presence ...
Camk1g - Camk1g (untagged ORF) - Rat calcium/calmodulin-dependent protein kinase IG (Camk1g), (10 ug) available for purchase from OriGene - Your Gene Company.
Camk2d2 antibody (calcium/calmodulin-dependent protein kinase (CaM kinase) II delta 2) for IHC-P, WB. Anti-Camk2d2 pAb (GTX124377) is tested in Zebrafish samples. 100% Ab-Assurance.
Plasmid pDONR223-CAMK1G from Dr. William Hahns lab contains the insert CAMK1G and is published in Nature. 2010 Nov 24. ():. This plasmid is available through Addgene.
TY - JOUR. T1 - Amphetamine activate protein kinase C and calcium/calmodulin-dependent protein kinase via NMDA receptor in primary cultures of rat cortical neurons. AU - Wu, Hsueh-Hsia. AU - Lee, Horng-Mo. PY - 1999. Y1 - 1999. M3 - Article. VL - 1. SP - 12. EP - 19. JO - New Taipei Journal of Medicine. JF - New Taipei Journal of Medicine. SN - 1562-4242. ER - ...
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1MXE: Structure of the Complex of Calmodulin with the Target Sequence of Calmodulin-Dependent Protein Kinase I: Studies of the Kinase Activation Mechanism
Affiliation:Kagawa University,Medicine,Professor,医学部,教授, Research Field:Metabolomics,Orthopaedic surgery,Functional biochemistry,General physiology,Medical systems, Keywords:PREB,insulin,糖尿病,glucose,グルコース,インスリン,CaM-KK,膵β細胞,Gas6,CaMKIV, # of Research Projects:9, # of Research Products:68
View Tg(Camk2a-tTA)1Mmay/0 Tg(tetO-APPSwInd)102Dbo/0 involves: C3H/HeJ * C57BL/6 * CBA: phenotypes, images, diseases, and references.
Three genes encoding different Ca2+/calmodulin-dependent protein kinases have been characterized in the wheat phytopathogenic fungus Stagonospora nodorum. The kinases were identified from the S. nodorum genome sequence on the basis of sequence homology to known Ca2+/calmodulin- dependent protein kinases. Expression analysis determined that each of the kinases was expressed during growth in vitro and also during infection. The onset of sporulation triggered increased transcript levels of each of the kinases, particularly CpkA where an 11-fold increase in expression was observed during sporulation in planta. The role of the kinases was further determined via a reverse genetics approach. The disruption of CpkA affected vegetative growth in vitro and also sporulation. The cpkA strains produced 20-fold less spores on complex media and were unable to sporulate on defined minimal media. Infection assays showed that CpkA was not required for lesion development but was essential for sporulation at the ...
CaMKII alpha antibody (calcium/calmodulin-dependent protein kinase II alpha) for ICC/IF, IHC-Fr, WB. Anti-CaMKII alpha pAb (GTX127939) is tested in Mouse, Rat samples. 100% Ab-Assurance.
________ By STEVEN REIVE www.wheelbasemedia.com Illustration By GREG PERRY, www.wheelbasemedia.com ________ You can sell a young mans car to an old man, he once said, in the springtime of a colorfu
TY - JOUR. T1 - Mutational analysis of Ca2+-independent autophosphorylation of calcium/calmodulin-dependent protein kinase II. AU - Mukherji, Sucheta. AU - Soderling, Thomas. PY - 1995/6/9. Y1 - 1995/6/9. N2 - Previous studies with synthetic peptides indicate that residues 290-309, corresponding to the calmodulin (CaM)-binding domain of Ca2+/CaM-dependent protein kinase II interact with the catalytic core of the enzyme as a pseudosubstrate (Colbran, R. J., Smith, M. K., Schworer, C. M., Fong, Y. L., and Soderling, T. R. (1989) J. Biol. Chem. 264, 4800-4804). In the present study, we attempted to locate the pseudosubstrate motif by generation or removal of potential substrate recognition sequences (RXXS/T) at selected positions using site-directed mutagenesis. Based on previous results, Arg297, Thr305/306, and Ser314 were selected as key residues. Single mutations such as N294S, K300S, A302R, A309R, and R311A were expressed, purified, and characterized. Several of the mutants exhibited decreased ...
Small molecule inhibitors of the human sirtuins and calmodulin-dependent protein kinases have shown promising anti-cancer activity in cell-based screens and animal models. We have synthesized analogues of these compounds, identifying more selective sirtuin inhibitors and more potent calmodulin-dependent protein kinase inhibitors.The sirtuins are a family of NAD+-dependent deacetylases that regulate cellular aging and gene silencing in simple organisms and appear to play important regulatory roles in human cells that make them attractive anti-cancer targets. We have previously identified the compound cambinol, an inhibitor of the human sirtuins SIRT1 and SIRT2, which is selectively toxic to Burkitts lymphoma cells. In order to determine which sirtuin is the relevant target, we screened analogues of cambinol, identifying compounds that exhibited moderate selectivity for both SIRT1 and SIRT2. The compound JP136 is ten-fold more selective in vitro for SIRT1 over SIRT2, with respective IC50s of 13 ...
Looking for online definition of CaM-kinase II alpha chain in the Medical Dictionary? CaM-kinase II alpha chain explanation free. What is CaM-kinase II alpha chain? Meaning of CaM-kinase II alpha chain medical term. What does CaM-kinase II alpha chain mean?
Authors Affiliations: 1Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Istituto di Endocrinologia e Oncologia Sperimentale del CNR Naples; 2Department of Neuroscience, Reproductive and Odontostomatological Sciences, University of Naples "Federico II", Naples; 3Department of Medicine-DIMED, Unit of Endocrinology, University of Padua, Padova; and 4Department of Medicine and Surgery, University of Salerno, Salerno, ...
CAMK2A - CAMK2A (Myc-DDK-tagged)-Human calcium/calmodulin-dependent protein kinase II alpha (CAMK2A), transcript variant 2 available for purchase from OriGene - Your Gene Company.
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
Tudjon meg többet a következőkről potassium-stannate-trihydrate. Támogatjuk a tudományt termékkínálatunkkal, szolgáltatásainkkal, kiváló folyamatainkkal és az ezeket megvalósító munkatársainkkal.
Background: CaMKK β is a major kinase activated by elevated levels of intracellular calcium. Our previous data has suggested that CaMKK β is neuroprotective after stroke in young mice as inhibition of this kinase aggravated stroke outcome. As aging is an important determinant of stroke outcomes, here we evaluated the functional role of CaMKK β in stroke in aged mice.. Methods: Aged wild type (WT) males received intracerebral injections of lentiviral vectors carrying either CaMKK β (LV-CaMKK β) or GFP (LV-GFP) 7 days before middle cerebral artery occlusion (MCAO, 90 minutes). Acute infarcts and neurological deficits scores were then analyzed at 72 hours post MCAO. In chronic survival studies, aged male CaMKK β knockout (KO) and WT mice were subjected to 60 minutes MCAO. Following stroke, long-term behavioral assessments were continuously performed for 3 weeks in KO and WT mice until the sacrifice for tissue loss assessments.. Results: Baseline levels of CaMKK β in aged brain were ...
Mouse eggs are ovulated following arrest at metaphase of the second meiotic division (metII). Fertilization breaks this arrest, with the egg extruding a second polar body (PB2) and forming pronuclei. Ca2+ spikes induced by phospholipase C zeta, which are introduced into the egg on gamete fusion, are responsible for causing the degradation of Erp1/Emi2 (Fbxo43 - Mouse Genome Informatics) (Ducibella and Fissore, 2008; Jones, 2005; Mehlmann, 2005; Swann et al., 2006). Erp1/Emi2 loss activates the anaphase-promoting complex (APC) and so drives exit from meiosis (Madgwick et al., 2006; Shoji et al., 2006).. In frog eggs, the Ca2+ fertilization signal switches on calmodulin-dependent protein kinase II (CamKII; Camk2), which phosphorylates Erp1/Emi2 and so promotes its degradation (Liu and Maller, 2005; Rauh et al., 2005; Schmidt et al., 2005). Consistent with this more recent development in the understanding of the molecular events of activation, it had been discovered several years previously, also ...
Neonatal hypoxia-ischemia (HI) is a major cause of death and disability in neonates. HI leads to a dramatic rise in intracellular calcium levels, which was originally thought to be detrimental to the brain. However, it has been increasingly recognized that this calcium signaling may also play an important protective role after injury by triggering endogenous neuroprotective pathways. Calcium/calmodulin-dependent protein kinase kinase ß (CaMKK ß) is a major kinase activated by elevated levels of intracellular calcium. Here we evaluated the functional role of CaMKK ß in neonatal mice after HI in both acute and chronic survival experiments. Postnatal day ten wild-type (WT) and CaMKK ß knockout (KO) mouse male pups were subjected to unilateral carotid artery ligation, followed by 40 min of hypoxia (10% O2 in N2). STO-609, a CaMKK inhibitor, was administered intraperitoneally to WT mice at 5 minutes after HI. TTC (2,3,5-triphenyltetrazolium chloride monohydrate) staining was used to assess ...
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Since its inception, the "synaptic tagging hypothesis" has inspired many to search for synaptic tags. However, very few molecules have been proposed as candidates (Frey and Frey, 2008). The nature and identity of PRPs and synaptic tags are under intensive investigations (Frey and Frey, 2008). Two pathway experiments showed that blockade of protein kinase A (PKA) or its interaction with A kinase-anchoring proteins (AKAPs) prevents synaptic capture, suggesting that PKA or its anchoring at active synapses may serve as a synaptic tag for L-LTP (Huang et al., 2006; Young et al., 2006). Calcium/calmodulin-dependent protein kinase II (CaMKII) is also implicated as an L-LTP-specific tag (Sajikumar et al., 2007). Studies thus far have been based on the use of pharmacological inhibitors in two-pathway experiments. However, it is necessary to show that these tags are transiently and locally activated in a protein synthesis-independent manner by weak stimulation. In a recent study, NMDA-dependent, ...
Expression of CAMK4 (CaMK-GR) in duodenum tissue. Antibody staining with HPA011753, HPA017206 and CAB004347 in immunohistochemistry.
Contact Us. Tel:732-484-9848. Fax:888-484-5008. Email:[email protected]. Add:1 Deer Park Dr, Suite Q,. Monmouth Junction, NJ 08852, USA. ...
Excessive activation of β-adrenergic, angiotensin II, and aldosterone signaling pathways promotes mortality after myocardial infarction (MI), while antagonist drugs targeting these pathways are core therapies for treating post-MI patients. The multifunctional calcium/calmodulin-dependent protein kinase II (CaMKII) is activated by catecholamines and angiotensin II, and CaMKII inhibition prevents isoproterenol- and angiotensin II-mediated cardiomyopathy. Here we ask the hypothesis if aldosterone and CaMKII participated in common responses to MI by developing a mouse MI model supplemented by aldosterone infusion (MI+Aldo) to approximate plasma aldosterone levels measured in MI patients. We find that aldosterone exerts direct toxic actions on myocardium by oxidative activation of CaMKII, causing cardiac rupture and increased mortality in mice after MI (65.5% for aldosterone versus 31.0% for vehicle, P=0.007, n≥19 mice per treatment). Aldosterone oxidizes CaMKII by recruiting NADPH oxidase, and ...
Nuclear factor-kappa beta (NF-kβ) pro-inflammatory signalling is important in modulating endothelial dysfunction and may be important in vascular dysfunction associated with the ageing process. Recent studies in the heart have highlighted Calcium/calmodulin-dependent protein kinase II (CaMKII) as a novel regulator of NF-kβ signalling. However nothing is known of the role this interaction could play in regulating dysfunction of the vasculature during ageing. Here we (i) characterise NF-kβ signalling in vascular endothelial cells and examine the potential for CaMKII modulation and (ii) determine whether CaMKIIδ expression is altered in ageing.. Using human umbilical vein endothelial cells (HUVECs) as an in vitro model system, initial experiments have established that pro-inflammatory NF-kB signalling is activated in response to both tumour necrosis factor α (TNF-α) and interleukin-1β (IL-1β) stimulation. This was shown by a significant reduction in IkBα expression (1.18 ± 0.16 vs. 0.48 ...
FUNCTION: ACTIVATES TYROSINE AND TRYPTOPHAN HYDROXYLASES IN THE PRESENCE OF CA(2+)/CALMODULIN-DEPENDENT PROTEIN KINASE II, AND STRONGLY ACTIVATES PROTEIN KINASE C. IS PROBABLY A MULTIFUNCTIONAL REGULATOR OF THE CELL SIGNALING PROCESSES MEDIATED BY BOTH KINASES ...
Involvement of PCNK in CaMKIα phosphorylation.MCF-7 cells were transfected with siRNA targeting PNCK or control siRNA (NT1) and 72 h later, cells were exposed
Expression of CAMK2A (CAMKA, CaMKIINalpha, KIAA0968) in ovary tissue. Antibody staining with HPA051783, HPA051785, HPA053973 and CAB004330 in immunohistochemistry.
Raval A, Tanner SM, Byrd JC, Angerman EB, Perko JD, Chen SS, Hackanson B, Grever MR, Lucas DM, Matkovic JJ, Lin TS, Kipps TJ, Murray F, Weisenburger D, Sanger W, Lynch J, Watson P, Jansen M, Yoshinaga Y, Rosenquist R, de Jong PJ, Coggill P, Beck S, Lynch H, de la Chapelle A, Plass C. Downregulation of death-associated protein kinase 1 (DAPK1) in chronic lymphocytic leukemia. Cell. 2007 Jun 01; 129(5):879-90 ...
Journal of Diabetes Research is a peer-reviewed, Open Access journal that publishes research articles, review articles, and clinical studies related to type 1 and type 2 diabetes. The journal welcomes submissions focusing on the epidemiology, etiology, pathogenesis, management, and prevention of diabetes, as well as associated complications, such as diabetic retinopathy, neuropathy and nephropathy.
C_horizon,C horizon]]:-> [[parent_rock,parent rock]]:母岩 ;[[C-value_paradox,C-value paradox]]: ;[[C3_pathway,C3 pathway (C,sub>3,/sub> pathway)]]:C3経路 ;[[C3_plant,C3 plant(s) [C,sub>3,/sub> plant(s)]]]:C3植物 ;[[C4_pathway,C4 pathway (C,sub>4,/sub> pathway)]]:C4経路 ;[[C4_photosynthesis,C4 photosynthesis (C,sub>4,/sub> photosynthesis)]] ;[[C4_plant,C4 plant(s) [C,sub>4,/sub> plant(s)]]]:C4植物 === Ca === ;[[Ca2+/calmodulin-dependent_protein_kinase , Ca,sup>2+,/sup>/calmodulin-dependent protein kinase]]:->[[CaM-kinase]] ;cactus:サボテン,サボテン類 ==== Cad ==== ;[[cadherin]]:カドヘリン ;[[caducous]]: ==== Cae ==== ;[[Caenorhabditis_elegans , Caenorhabditis elegans]]: ==== Cag ==== ;[[caged_molecule,caged molecule]]: ==== Cak ==== ;[[CAK]]:->[[Cdk-activating_kinase , Cdk-activating kinase]] ==== Cal ==== ;[[calcitonin]]: ;[[calcium_pump,calcium pump]]:->[[Ca2+_pump , Ca,sup>2+,/sup> pump]] ;[[calcrete,calcrete (caliche)]]: ;caliche:-> [[calcrete,calcrete]] ...
RecName: Full=Phosphatidylinositol 5-phosphate 4-kinase type-2 gamma; EC=2.7.1.149;AltName: Full=Phosphatidylinositol 5-phosphate 4-kinase type II gamma; Short=PI(5)P 4-kinase type II gamma; Short=PIP4KII-gamma ...
Human CAMK2D full-length ORF ( NP_742126.1, 1 a.a. - 478 a.a.) recombinant protein with GST-tag at N-terminal. (H00000817-P01) - Products - Abnova

Camk2a - Calcium/calmodulin-dependent protein kinase type II subunit alpha - Mus musculus (Mouse) - Camk2a gene & proteinCamk2a - Calcium/calmodulin-dependent protein kinase type II subunit alpha - Mus musculus (Mouse) - Camk2a gene & protein

Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and ... is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. ... Calcium/calmodulin-dependent protein kinase type II subunit alpha (EC:2.7.11.17By similarity. ,p>Manually curated information ... View protein in PROSITE. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 ...
more infohttp://www.uniprot.org/uniprot/P11798-2

KEGG BRITE: KEGG Orthology (KO) - Tupaia chinensis (Chinese tree shrew)KEGG BRITE: KEGG Orthology (KO) - Tupaia chinensis (Chinese tree shrew)

102479520 CAMK4; calcium/calmodulin dependent protein kinase IV 102477502 LIPE; lipase E, hormone sensitive type 102484417 LDLR ... 102473558 CAMK1; calcium/calmodulin dependent protein kinase I 102478578 CAMK1D; calcium/calmodulin dependent protein kinase ID ... 102500231 CAMK1G; calcium/calmodulin dependent protein kinase IG 102503259 CAMK2G; calcium/calmodulin dependent protein kinase ... calcium/calmodulin-dependent protein kinase I [EC:2.7.11.17] K08794 CAMK1; calcium/calmodulin-dependent protein kinase I [EC: ...
more infohttp://www.genome.jp/kegg-bin/get_htext?tup00001+102491327

Calcium-Calmodulin-Dependent Protein Kinases | Colorado PROFILESCalcium-Calmodulin-Dependent Protein Kinases | Colorado PROFILES

Calcium-Calmodulin-Dependent Protein Kinase Type 1. *Calcium-Calmodulin-Dependent Protein Kinase Type 2 ... Calcium-Calmodulin-Dependent Protein Kinases*Calcium-Calmodulin-Dependent Protein Kinases. *Protein Kinases, Calcium-Calmodulin ... Multifunctional Calcium-Calmodulin-Dependent Protein Kinases*Multifunctional Calcium-Calmodulin-Dependent Protein Kinases ... Restricted Calcium-Calmodulin-Dependent Protein Kinases*Restricted Calcium-Calmodulin-Dependent Protein Kinases ...
more infohttps://profiles.ucdenver.edu/display/204113

Human Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4) Protein (His tag), Recombinant | ABIN668012Human Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4) Protein (His tag), Recombinant | ABIN668012

Protein (His tag). Spezies: Human. Quelle: Escherichia coli (E. coli). Jetzt Produkt ABIN668012 bestellen. ... Recombinant Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4) ... Synonyms: Calcium/calmodulin-dependent protein kinase type IV, caMK, CaMK IV, CaMK-GR, IV. NCBI no.: NP_001735 ... Calcium/calmodulin-Dependent Protein Kinase Kinase 1, alpha Proteine * Calcium/calmodulin-Dependent Protein Kinase Kinase 2, ...
more infohttp://www.antikoerper-online.de/protein/668012/Calcium/calmodulin-Dependent+Protein+Kinase+IV+CAMK4+AA+1-473+protein+His+tag/

PKCA - Raf-1 inhibitor GW5074 Sustained Morphine Treatment AugmentsPKCA - Raf-1 inhibitor GW5074 Sustained Morphine Treatment Augments

Death-associated protein kinase (DAPK) is really a calmodulin-regulated serine/threonine kinase and August 2, 2018. by Felix ... Syk Kinase *T-Type Calcium Channels *Transient Receptor Potential Channels *TRPP *Uncategorized ... Louis, MO, USA), and macrophage, neutrophil, and lymphocyte quantities were dependant on keeping track of 400 cells in ... Death-associated protein kinase (DAPK) is really a calmodulin-regulated serine/threonine kinase and possesses apoptotic and ...
more infohttp://gw5074.com/tag/pkca/

Recombinant Human CAMKIV protein (ab60756) | AbcamRecombinant Human CAMKIV protein (ab60756) | Abcam

Ab60756 is a full length protein produced in Baculovirus infected Sf9 cells and has been validated in FuncS… ... Calcium / calmodulin dependent protein kinase type 4 catalytic chain. *Calcium / calmodulin dependent protein kinase type IV ... Calcium/calmodulin dependent protein kinase type IV. *Calcium/calmodulin-dependent protein kinase type IV ... Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in ...
more infohttp://www.abcam.com/recombinant-human-camkiv-protein-ab60756.html

Cyclic AMP Response Element Modulator
     Summary Report | CureHunterCyclic AMP Response Element Modulator Summary Report | CureHunter

Calcium-Calmodulin-Dependent Protein Kinase Type 4 5. Proteasome Endopeptidase Complex (Proteasome) ... CREM Protein, alpha Isoform; CREM Protein, epsilon Isoform; CREM Protein, gamma Isoform; CREM Protein, tau Isoform; ICERIIgamma ... Amino Acids, Peptides, and Proteins*Proteins: 90489*Transcription Factors: 20597*Basic-Leucine Zipper Transcription Factors: 21 ... DNA-Binding Proteins: 1111*Basic-Leucine Zipper Transcription Factors: 21*Cyclic AMP Response Element Modulator: 16*human CREM ...
more infohttp://www.curehunter.com/public/keywordSummaryD051786.do

calcium/calmodulin-dependent protein kinase type II subunit alpha isof - Protein - NCBIcalcium/calmodulin-dependent protein kinase type II subunit alpha isof - Protein - NCBI

calcium/calmodulin-dependent protein kinase type II subunit alpha isoform 2 [Hom... calcium/calmodulin-dependent protein kinase ... calcium/calmodulin-dependent protein kinase type II subunit alpha isoform 2 [Homo sapiens]. NCBI Reference Sequence: NP_ ... The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein ... Transcript/Protein Information [PANTHER Classification System] Transcript/Protein Information. PANTHER Classification System ...
more infohttps://www.ncbi.nlm.nih.gov/protein/NP_741960

CAMK1G - Calcium/calmodulin-dependent protein kinase type 1G - Homo sapiens (Human) - CAMK1G gene & proteinCAMK1G - Calcium/calmodulin-dependent protein kinase type 1G - Homo sapiens (Human) - CAMK1G gene & protein

Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro ... View protein in PROSITE. PS00107 PROTEIN_KINASE_ATP, 1 hit. PS50011 PROTEIN_KINASE_DOM, 1 hit. PS00108 PROTEIN_KINASE_ST, 1 ... View protein in PROSITE. PS00107 PROTEIN_KINASE_ATP, 1 hit. PS50011 PROTEIN_KINASE_DOM, 1 hit. PS00108 PROTEIN_KINASE_ST, 1 ... Calcium/calmodulin-dependent protein kinase type 1G (EC:2.7.11.17). Alternative name(s): ...
more infohttps://www.uniprot.org/uniprot/Q96NX5

Behavioral and Structural Responses to Chronic Cocaine Require a Feedforward Loop Involving ΔFosB and Calcium/Calmodulin...Behavioral and Structural Responses to Chronic Cocaine Require a Feedforward Loop Involving ΔFosB and Calcium/Calmodulin...

2004) Protein phosphatases and calcium/calmodulin-dependent protein kinase II-dependent synaptic plasticity. J Neurosci 24:8404 ... dopamine type-1 receptor and protein kinase A dependence. Eur J Neurosci 31:1243-1251. ... Calcium/calmodulin-dependent protein kinase II (CaMKII) is a highly expressed serine/threonine kinase whose α and β isoforms ... 2003) Calcium/calmodulin-dependent protein kinase II contributes to activity-dependent filopodia growth and spine formation. J ...
more infohttp://www.jneurosci.org/content/33/10/4295.full

An update on nuclear calcium signalling | Journal of Cell ScienceAn update on nuclear calcium signalling | Journal of Cell Science

... interaction and modulation by calcium/calmodulin-dependent protein kinase II. J. Biol. Chem. 280, 15912-15920. ... Signaling to the nucleus by an L-type calcium channel-calmodulin complex through the MAP kinase pathway. Science 294, 333-339. ... Proteins in excess of ∼50 kDa require energy-dependent movement across the NPC. Such large proteins are often designated for ... Calmodulin does not contain an obvious motif for nuclear localisation, and might therefore `piggy-back on other proteins that ...
more infohttp://jcs.biologists.org/content/122/14/2337

Plus itPlus it

2010) Transient viral-mediated overexpression of alpha-calcium/calmodulin-dependent protein kinase II in the nucleus accumbens ... dopamine type-1 receptor and protein kinase A dependence. Eur J Neurosci 31:1243-1251, doi:10.1111/j.1460-9568.2010.07155.x, ... responses to chronic cocaine require a feedforward loop involving DeltaFosB and calcium/calmodulin-dependent protein kinase ii ... 2010) Transient overexpression of alpha-Ca2+/calmodulin-dependent protein kinase II in the nucleus accumbens shell enhances ...
more infohttp://www.jneurosci.org/content/34/34/11461

RCSB PDB - Protein Feature View 









 - Calcium/calmodulin-dependent protein kinase type II subunit beta - Q13554 (KCC2B...RCSB PDB - Protein Feature View - Calcium/calmodulin-dependent protein kinase type II subunit beta - Q13554 (KCC2B...

The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex ... Calcium/calmodulin-dependent protein kinase type II subunit beta - Q13554 (KCC2B_HUMAN) ... Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation ... The CAMK2 protein kinases contain a unique C-terminal subunit association domain responsible for oligomerization. UniProt ...
more infohttp://www.rcsb.org/pdb/protein/Q9UGH9

Nuclear calcineurin is a sensor for detecting Ca2+ release from the nuclear envelope via IP3R | Springer for Research &...Nuclear calcineurin is a sensor for detecting Ca2+ release from the nuclear envelope via IP3R | Springer for Research &...

... interaction and modulation by calcium/calmodulin-dependent protein kinase II. J Biol Chem 280:15912-15920CrossRefGoogle Scholar ... Signaling to the nucleus by an L-type calcium channel-calmodulin complex through the MAP kinase pathway. Science 294:333-339 ... Klee CB, Ren H, Wang X (1998) Regulation of the calmodulin-stimulated protein phosphatase, calcineurin. J Biol Chem 273:13367- ... Perrino BA, Ng LY, Soderling TR (1995) Calcium regulation of calcineurin phosphatase activity by its B subunit and calmodulin. ...
more infohttps://rd.springer.com/article/10.1007%2Fs00109-018-1701-2

Sarcoplasmic Reticulum and Nuclear Envelope Are One Highly Interconnected Ca2+ Store Throughout Cardiac Myocyte | Circulation...Sarcoplasmic Reticulum and Nuclear Envelope Are One Highly Interconnected Ca2+ Store Throughout Cardiac Myocyte | Circulation...

... interaction and modulation by calcium/calmodulin-dependent protein kinase II. J Biol Chem. 2005; 280: 15912-15920. ... In some cell types (nonmuscle cells), it is generally accepted that the outer nuclear membrane and ER have similar ... Ca2+ binding effects on protein conformation and protein interactions of canine cardiac calsequestrin. J Biol Chem. 1988; 263: ... Subramanian K, Meyer T. Calcium-induced restructuring of nuclear envelope and endoplasmic reticulum calcium stores. Cell. 1997 ...
more infohttp://circres.ahajournals.org/content/99/3/283

Calcium/calmodulin-dependent protein kinase type IV (CaMKIV) inhibits apoptosis induced by potassium deprivation in cerebellar...Calcium/calmodulin-dependent protein kinase type IV (CaMKIV) inhibits apoptosis induced by potassium deprivation in cerebellar...

These data are compatible with a model where Ca2+ influx via L-type Ca2+ channels prevents caspase-dependent cleavage of CaMKIV ... We present evidence that blockade of L-type Ca2+ channels (nifedipine sensitive) but not N/P/Q-type Ca2+ channels (omega- ... The entry into apoptosis was associated with a progressive caspase-3-dependent cleavage of CaMKIV, but not of CaMKII. CaMKIV ... and promotes neuronal survival by maintaining a constitutive level of CaMKIV/CREB-dependent gene expression. ...
more infohttps://www.semanticscholar.org/paper/Calcium%2Fcalmodulin-dependent-protein-kinase-type-IV-S%C3%A9e-Boutillier/1e1a5dee5c6ea8f4fea63460969400df7a8bb268

CAMK4 Gene - GeneCards | KCC4 Protein | KCC4 AntibodyCAMK4 Gene - GeneCards | KCC4 Protein | KCC4 Antibody

Protein Coding), Calcium/Calmodulin Dependent Protein Kinase IV, including: function, proteins, disorders, pathways, orthologs ... Calcium/Calmodulin-Dependent Protein Kinase Type IV Catalytic Chain 2 3 * Brain Ca++-Calmodulin-Dependent Protein Kinase Type ... Protein Symbol:. Q16566-KCC4_HUMAN. Recommended name:. Calcium/calmodulin-dependent protein kinase type IV Protein Accession:. ... protein kinase,type IV,calcium/calmodulin dependent,brain *CAMK4. UniProtKB/Swiss-Prot CatalyticActivity: ATP + a protein = ADP ...
more infohttp://www.genecards.org/cgi-bin/carddisp.pl?gene=CAMK4

Richard Goodman - Publications
     - Oregon Health & Science UniversityRichard Goodman - Publications - Oregon Health & Science University

Calcium-Calmodulin-Dependent Protein Kinase Type 4 * Transcriptional Activation * Phosphorylation * N-Methylaspartate ... The cAMP-regulated enhancer-binding protein ATF-1 activates transcription in response to cAMP-dependent protein kinase A. ... The catalytic subunit of cAMP-dependent protein kinase induces expression of genes containing cAMP-responsive enhancer elements ... Protein kinase a directly regulates the activity and proteolysis of cubitus interruptus. Chen, Y., Gallaher, N., Goodman, R. H. ...
more infohttps://ohsu.pure.elsevier.com/en/persons/richard-goodman/publications/?page=2&ordering=title&descending=false

anti-CaMKIIdelta antibody | Rabbit CaMKIIdelta Polyclonal Antibody-NP 742127.1anti-CaMKIIdelta antibody | Rabbit CaMKIIdelta Polyclonal Antibody-NP 742127.1

CAMK2D; CAMKD; Calcium/calmodulin-dependent protein kinase type II subunit delta; CaM kinase II subunit delta; CaMK-II subunit ... Calcium/calmodulin-dependent protein kinase involved in the regulation of Ca2+ homeostatis and excitation-contraction coupling ... The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein ... NCBI Protein Information. calcium/calmodulin-dependent protein kinase type II subunit delta ...
more infohttps://www.mybiosource.com/polyclonal-human-mouse-rat-antibody/camkiidelta/2537731

Prenatal diagnosis of fetal cardiac rhabdomyoma associated with tuberous sclerosis: A case reportPrenatal diagnosis of fetal cardiac rhabdomyoma associated with tuberous sclerosis: A case report

BicC family RNA binding protein 1; BSA: bovine serum albumin; CAMKK2 kinase: calcium/calmodulin dependent protein kinase kinase ... deiodinase iodothyronine type III (Dio3) locus are upregulated in an mTORC1-dependent manner. Sustained mTORC1 signaling during ... AMP-activated protein kinase; ARMC8: armadillo repeat containing 8; ATP: adenosine triphosphate; BafA1: bafilomycin A1; BCAA: ... Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by high throughput sequencing Mus musculus Source Type: research ...
more infohttps://medworm.com/561603159/prenatal-diagnosis-of-fetal-cardiac-rhabdomyoma-associated-with-tuberous-sclerosis-a-case-report/

IMP: Integrative Multi-species PredictionIMP: Integrative Multi-species Prediction

protein tyrosine phosphatase, non-receptor type 14. 0.207. Camkk2. calcium/calmodulin-dependent protein kinase kinase 2, beta. ... catenin (cadherin-associated protein), delta 2 (neural plakophilin-related arm-repeat protein). 0.319. ... fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor). 0.952. ... ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1). 0.793. ...
more infohttp://imp.princeton.edu/predictions/process_ortho/yeast-context-global/13491/?gene=26583

9L Gliosarcoma Cell Proliferation and Tumor Growth in Rats Are Suppressed by N-Hydroxy-N′-(4-butyl-2-methylphenol) Formamidine ...9L Gliosarcoma Cell Proliferation and Tumor Growth in Rats Are Suppressed by N-Hydroxy-N′-(4-butyl-2-methylphenol) Formamidine ...

... acid mediates calcium/calmodulin-dependent protein kinase II-induced mitogen-activated protein kinase activation in vascular ... 20-HETE has been implicated as playing a role in promoting the growth of several cell types (Roman et al., 2002). Blockade of ... which is generated after activation of phospholipase A2 by calcium/calmodulin protein kinase II. This suggests that 20-HETE ... with a fall in p42/p44 mitogen-activated protein kinase and stress-activated protein kinase/c-Jun NH2-terminal kinase ...
more infohttp://jpet.aspetjournals.org/content/317/1/97

The controversial role of phospholipase C epsilon (PLCε) in cancer development and progressionThe controversial role of phospholipase C epsilon (PLCε) in cancer development and progression

RAS p21 protein activator 2; CAMK2B - calcium/calmodulin dependent protein kinase II beta; DAB2IP - disabled 2 (DAB2) ... interacting protein; NF1 - neurofibromin 1; PI3KC3 - phosphatidylinositol 3-kinase catalytic subunit type 3; PI3KCA - ... Novel role of pleckstrin homology domain of the Bcr-Abl protein: analysis of protein-protein and protein-lipid interactions. ... PLC proteins can be activated by the receptor tyrosine kinases (RTK) or G-protein coupled receptors (GPCR) in response to the ...
more infohttp://www.jcancer.org/v08p0716.htm

The δ<sub>c</sub> isoform of CaMKII is activated in cardiac hypertrophy and induces dilated cardiomyopathy and heart...The δ<sub>c</sub> isoform of CaMKII is activated in cardiac hypertrophy and induces dilated cardiomyopathy and heart...

Calcium-Calmodulin-Dependent Protein Kinase Type 4 Ryanodine Receptor Calcium Release Channel ... Recent studies have demonstrated that transgenic (TG) expression of either Ca2+/calmodulin-dependent protein kinase IV (CaMKIV ... keywords = "Ca/calmodulin-dependent protein kinase, Dilated cardiomyopathy, Heart failure, Transgenic mice", ... N2 - Recent studies have demonstrated that transgenic (TG) expression of either Ca2+/calmodulin-dependent protein kinase IV ( ...
more infohttps://ucdavis.pure.elsevier.com/en/publications/the-%CE%B4subcsub-isoform-of-camkii-is-activated-in-cardiac-hypertroph

The multifunctional Ca2+/calmodulin-dependent protein kinases.  - PubMed - NCBIThe multifunctional Ca2+/calmodulin-dependent protein kinases. - PubMed - NCBI

Calcium-Calmodulin-Dependent Protein Kinase Type 4. *Calcium-Calmodulin-Dependent Protein Kinases* ... Multifunctional Ca2+/calmodulin-dependent (CaM) kinase, CaM kinase Ia, CaM kinase Ib and CaM kinase IV are four of the kinases ... The multifunctional Ca2+/calmodulin-dependent protein kinases.. Schulman H1.. Author information. 1. Department of Pharmacology ... Calcium-Calmodulin-Dependent Protein Kinase Type 4. *Calcium-Calmodulin-Dependent Protein Kinases ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/8507497?dopt=Abstract
  • The transcription factor ΔFosB and the brain-enriched calcium/calmodulin-dependent protein kinase II (CaMKIIα) are induced in the nucleus accumbens (NAc) by chronic exposure to cocaine or other psychostimulant drugs of abuse, in which the two proteins mediate sensitized drug responses. (jneurosci.org)
  • Here, we demonstrate that ΔFosB is phosphorylated by CaMKIIα at the protein-stabilizing Ser27 and that CaMKII is required for the cocaine-mediated accumulation of ΔFosB in rat NAc. (jneurosci.org)
  • Conversely, we show that ΔFosB is both necessary and sufficient for cocaine induction of CaMKIIα gene expression in vivo , an effect selective for D 1 -type MSNs in the NAc shell subregion. (jneurosci.org)
  • Furthermore, induction of dendritic spines on NAc MSNs and increased behavioral responsiveness to cocaine after NAc overexpression of ΔFosB are both CaMKII dependent. (jneurosci.org)
  • These data establish that ΔFosB and CaMKII engage in a cell-type- and brain-region-specific positive feedforward loop as a key mechanism for regulating the reward circuitry of the brain in response to chronic cocaine. (jneurosci.org)
  • 5 Notably, this pathway could only be activated by Ca 2+ from local InsP 3 R (not global Ca 2+ transients), presumably because calmodulin and CaMKII physically associate with the InsP 3 R at the NucEn. (ahajournals.org)
  • Recent studies have demonstrated that transgenic (TG) expression of either Ca 2+ /calmodulin-dependent protein kinase IV (CaMKIV) or CaMKIIδ B , both of which localize to the nucleus, induces cardiac hypertrophy. (elsevier.com)
  • Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. (uniprot.org)
  • In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. (rcsb.org)
  • This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. (rcsb.org)
  • Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. (mybiosource.com)
  • Bidirectional plasticity of calcium-permeable AMPA receptors in oligodendrocyte lineage cells. (abcam.com)
  • It interacts with the transcription factor TBR1 and binds to several cell-surface proteins including amyloid precursor protein, neurexins and syndecans. (wikipedia.org)
  • p>When browsing through different UniProt proteins, you can use the 'basket' to save them, so that you can back to find or analyse them later. (uniprot.org)
  • They observed that targeting the Ca 2+ -binding protein parvalbumin to the nucleus significantly reduced cell proliferation and altered the proportions of cells that were in the S and G 2 -M phases of the cell cycle. (biologists.org)
  • The present study examined the effects of N- hydroxy- N ′-(4-butyl-2 methylphenyl) formamidine (HET0016), a selective inhibitor of the formation of 20-hydroxyeicosatrienoic acid (20-HETE) on the growth of 9L rat gliosarcoma cells in vitro and in vivo. (aspetjournals.org)
  • CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. (uniprot.org)
  • CAMK2 is composed of 4 different chains: alpha ( CAMK2A ), beta ( CAMK2B ), gamma ( CAMK2G ), and delta ( CAMK2D ). (rcsb.org)
  • May play a role in the consolidation/retention of hippocampus-dependent long-term memory. (abcam.com)
  • May anchor the PKA protein to cytoskeletal and/or organelle-associated proteins, targeting the signal carried by cAMP to specific intracellular effectors. (genecards.org)
  • Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed. (uniprot.org)
  • View conserved domains detected in this protein sequence using CD-search. (nih.gov)
  • See 5 reference sequence protein isoforms for the CAMK2A gene. (nih.gov)
  • Shortly after, mammalian homologues of PLC210 have been independently identified by three groups by screening of rat and human expressed sequence tag databases [ 4 - 6 ]. (jcancer.org)
  • Here we used inhibitory component of AP-1 transcription factor, JunB, in order to map brain regions where JunB-dependent transcription is regulated during consolidation and extinction of contextual fear memory. (readbyqxmd.com)
  • Association of CamK2A genetic variants with transition time from occasional to regular heroin use in a sample of heroin-dependent individuals. (nih.gov)
  • The tool works with standard single letter nucleotide or protein codes including ambiguities and can match Prosite patterns in protein sequences. (nih.gov)
  • The aim of this review is to provide a comprehensive and up-to-date overview of the experimental and clinical data about the role of PLCε in the development and progression of the different types of human and experimental tumors. (jcancer.org)
  • In addition, DBM inhibited NADPH oxidase 2 and 4 expression and oxidative DNA damage. (bioscientifica.com)
  • Although importance of PLC enzymes for key cellular functions is well established, the PLC proteins belonging to the ε, ζ and η subfamilies were identified and characterized only during the last decade. (jcancer.org)
  • IP3 stimulates Ca 2+ signaling and DAG acts through protein kinase C (PKC) leading to different cellular events, such as proliferation, growth and migration. (jcancer.org)
  • 4 The NucEn surrounds the nucleus and has both an inner and an outer membrane and a space in between where [Ca 2+ ] can be millimolar. (ahajournals.org)
  • Clarifying a possible role of calcium signaling and mechanisms involved may increase our understanding of how air pollution contributes to CVD. (springer.com)
  • In addition, the relatively rigid sarcomeric organization in striated muscle with a dense protein mesh at the Z-lines raises the possibility that local SR within a given sarcomere could serve its particular sarcomere but not necessarily communicate with neighboring sarcomeres either longitudinally or transversely. (ahajournals.org)
  • 1 Electrical depolarization of the cell membrane opens L-type Ca 2+ channels, causing Ca 2+ influx. (ahajournals.org)
  • Ang II" representative movie of a fluo-4 loaded isolated adult ventricular mouse cardiomyocyte. (springer.com)