A multifunctional calcium-calmodulin-dependent protein kinase subtype that occurs as an oligomeric protein comprised of twelve subunits. It differs from other enzyme subtypes in that it lacks a phosphorylatable activation domain that can respond to CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASE KINASE.
Destruction by passage of a galvanic electric current, as in disintegration of a chemical compound in solution.
A CALMODULIN-dependent enzyme that catalyzes the phosphorylation of proteins. This enzyme is also sometimes dependent on CALCIUM. A wide range of proteins can act as acceptor, including VIMENTIN; SYNAPSINS; GLYCOGEN SYNTHASE; MYOSIN LIGHT CHAINS; and the MICROTUBULE-ASSOCIATED PROTEINS. (From Enzyme Nomenclature, 1992, p277)
Toluenes in which one hydrogen of the methyl group is substituted by an amino group. Permitted are any substituents on the benzene ring or the amino group.
A monomeric calcium-calmodulin-dependent protein kinase subtype that is primarily expressed in neuronal tissues; T-LYMPHOCYTES and TESTIS. The activity of this enzyme is regulated by its phosphorylation by CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASE KINASE.
A class of ionotropic glutamate receptors characterized by affinity for N-methyl-D-aspartate. NMDA receptors have an allosteric binding site for glycine which must be occupied for the channel to open efficiently and a site within the channel itself to which magnesium ions bind in a voltage-dependent manner. The positive voltage dependence of channel conductance and the high permeability of the conducting channel to calcium ions (as well as to monovalent cations) are important in excitotoxicity and neuronal plasticity.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
A monomeric calcium-calmodulin-dependent protein kinase subtype that is expressed in a broad variety of mammalian cell types. Its expression is regulated by the action of CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASE KINASE. Several isoforms of this enzyme subtype are encoded by distinct genes.
A regulatory calcium-calmodulin-dependent protein kinase that specifically phosphorylates CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASE TYPE 1; CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASE TYPE 2; CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASE TYPE 4; and PROTEIN KINASE B. It is a monomeric enzyme that is encoded by at least two different genes.
Damage to the MYOCARDIUM resulting from MYOCARDIAL REPERFUSION (restoration of blood flow to ischemic areas of the HEART.) Reperfusion takes place when there is spontaneous thrombolysis, THROMBOLYTIC THERAPY, collateral flow from other coronary vascular beds, or reversal of vasospasm.
Adverse functional, metabolic, or structural changes in ischemic tissues resulting from the restoration of blood flow to the tissue (REPERFUSION), including swelling; HEMORRHAGE; NECROSIS; and damage from FREE RADICALS. The most common instance is MYOCARDIAL REPERFUSION INJURY.
A ubiquitous casein kinase that is comprised of two distinct catalytic subunits and dimeric regulatory subunit. Casein kinase II has been shown to phosphorylate a large number of substrates, many of which are proteins involved in the regulation of gene expression.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Irregular microscopic structures consisting of cords of endocrine cells that are scattered throughout the PANCREAS among the exocrine acini. Each islet is surrounded by connective tissue fibers and penetrated by a network of capillaries. There are four major cell types. The most abundant beta cells (50-80%) secrete INSULIN. Alpha cells (5-20%) secrete GLUCAGON. PP cells (10-35%) secrete PANCREATIC POLYPEPTIDE. Delta cells (~5%) secrete SOMATOSTATIN.
A 51-amino acid pancreatic hormone that plays a major role in the regulation of glucose metabolism, directly by suppressing endogenous glucose production (GLYCOGENOLYSIS; GLUCONEOGENESIS) and indirectly by suppressing GLUCAGON secretion and LIPOLYSIS. Native insulin is a globular protein comprised of a zinc-coordinated hexamer. Each insulin monomer containing two chains, A (21 residues) and B (30 residues), linked by two disulfide bonds. Insulin is used as a drug to control insulin-dependent diabetes mellitus (DIABETES MELLITUS, TYPE 1).
A type of pancreatic cell representing about 50-80% of the islet cells. Beta cells secrete INSULIN.
A benign tumor of the PANCREATIC BETA CELLS. Insulinoma secretes excess INSULIN resulting in HYPOGLYCEMIA.
A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.
A heat-stable, low-molecular-weight activator protein found mainly in the brain and heart. The binding of calcium ions to this protein allows this protein to bind to cyclic nucleotide phosphodiesterases and to adenyl cyclase with subsequent activation. Thereby this protein modulates cyclic AMP and cyclic GMP levels.
A family of enzymes that catalyze the conversion of ATP and a protein to ADP and a phosphoprotein.
An essential amino acid that is necessary for normal growth in infants and for NITROGEN balance in adults. It is a precursor of INDOLE ALKALOIDS in plants. It is a precursor of SEROTONIN (hence its use as an antidepressant and sleep aid). It can be a precursor to NIACIN, albeit inefficiently, in mammals.
An enzyme that phosphorylates myosin light chains in the presence of ATP to yield myosin-light chain phosphate and ADP, and requires calcium and CALMODULIN. The 20-kDa light chain is phosphorylated more rapidly than any other acceptor, but light chains from other myosins and myosin itself can act as acceptors. The enzyme plays a central role in the regulation of smooth muscle contraction.
Measurement of the intensity and quality of fluorescence.
Proteins which bind calmodulin. They are found in many tissues and have a variety of functions including F-actin cross-linking properties, inhibition of cyclic nucleotide phosphodiesterase and calcium and magnesium ATPases.
The property of emitting radiation while being irradiated. The radiation emitted is usually of longer wavelength than that incident or absorbed, e.g., a substance can be irradiated with invisible radiation and emit visible light. X-ray fluorescence is used in diagnosis.

Modulation of Ca2+/calmodulin-dependent protein kinase II activity by acute and chronic morphine administration in rat hippocampus: differential regulation of alpha and beta isoforms. (1/1822)

Calcium/calmodulin-dependent protein kinase II (CaMK II) has been shown to be involved in the regulation of opioid receptor signaling. The present study showed that acute morphine treatment significantly increased both Ca2+/calmodulin-independent and Ca2+/calmodulin-dependent activities of CaMK II in the rat hippocampus, with little alteration in the protein level of either alpha or beta isoform of CaMK II. However, chronic morphine treatment, by which rats were observed to develop apparent tolerance to morphine, significantly down-regulated both Ca2+/calmodulin-independent and Ca2+/calmodulin-dependent activities of CaMK II and differentially regulated the expression of alpha and beta isoforms of CaMK II at protein and mRNA levels. Application of naloxone or discontinuation of morphine treatment after chronic morphine administration, which induced the withdrawal syndrome of morphine, resulted in the overshoot of CaMK II (at both protein and mRNA levels) and its kinase activity. The phenomena of overshoot were mainly observed in the beta isoform of CaMK II but not in the alpha isoform. The effects of both acute and chronic morphine treatments on CaMK II could be completely abolished by the concomitant application of naloxone, indicating that the effects of morphine were achieved through activation of opioid receptors. Our data demonstrated that both acute and chronic morphine treatments could effectively modulate the activity and the expression of CaMK II in the hippocampus.  (+info)

Muscarinic stimulation of calcium/calmodulin-dependent protein kinase II in isolated rat pancreatic acini. (2/1822)

AIM: To study whether M3 receptor occupation would lead to activation of calcium/calmodulin-dependent protein kinase II (CaM kinase II). METHODS: In this study, we isolated rat pancreatic acini by collagenase digestion; measured the Ca2+/calmodulin-independent activity of autophosphorylated form of the CaM kinase II both before and after stimulation of the acini with muscarinic secretagogue bethanechol (Bet). RESULTS: Bet stimulated the activation of, or generation of Ca(2+)-independent activity of, this kinase, in a concentration (0.0001-1 mmol.L-1) and time (5-300 s)-dependent manner; with Bet of 100 mumol.L-1, Ca(2+)-independent activity increased from an unstimulated level of 4.5 +/- 0.3 (n = 4) to 8.9 +/- 1.3 (n = 4, P < 0.05) at 5 s. Another Ca2+ mobilizing secretagogue cholecystokinin (CCK) also activated the kinase; at 1 mumol.L-1, CCK increased Ca(2+)-independent kinase activity to 12.9 +/- 0.5 (n = 6, P < 0.05). Vasoactive intestinal peptide (VIP) at 1 mumol.L-1 did not produce significant Ca(2+)-independent kinase activity (from control 3.90 +/- 0.28 to 4.53 +/- 0.47, n = 6, P > 0.05). Atropine completely blocked Bet activation of the kinase. CONCLUSION: CaM kinase II plays a pivotal role in digestive enzyme secretion, especially during the initial phase of amylase secretion.  (+info)

Calcium/calmodulin-dependent phosphorylation and activation of human Cdc25-C at the G2/M phase transition in HeLa cells. (3/1822)

The human tyrosine phosphatase (p54(cdc25-c)) is activated by phosphorylation at mitosis entry. The phosphorylated p54(cdc25-c) in turn activates the p34-cyclin B protein kinase and triggers mitosis. Although the active p34-cyclin B protein kinase can itself phosphorylate and activate p54(cdc25-c), we have investigated the possibility that other kinases may initially trigger the phosphorylation and activation of p54(cdc25-c). We have examined the effects of the calcium/calmodulin-dependent protein kinase (CaM kinase II) on p54(cdc25-c). Our in vitro experiments show that CaM kinase II can phosphorylate p54(cdc25-c) and increase its phosphatase activity by 2.5-3-fold. Treatment of a synchronous population of HeLa cells with KN-93 (a water-soluble inhibitor of CaM kinase II) or the microinjection of AC3-I (a specific peptide inhibitor of CaM kinase II) results in a cell cycle block in G2 phase. In the KN-93-arrested cells, p54(cdc25-c) is not phosphorylated, p34(cdc2) remains tyrosine phosphorylated, and there is no increase in histone H1 kinase activity. Our data suggest that a calcium-calmodulin-dependent step may be involved in the initial activation of p54(cdc25-c).  (+info)

Calcium/calmodulin-dependent protein kinase II is associated with the N-methyl-D-aspartate receptor. (4/1822)

The molecular basis of long-term potentiation (LTP), a long-lasting change in synaptic transmission, is of fundamental interest because of its implication in learning. Usually LTP depends on Ca2+ influx through postsynaptic N-methyl-D-aspartate (NMDA)-type glutamate receptors and subsequent activation of Ca2+/calmodulin-dependent protein kinase II (CaMKII). For a molecular understanding of LTP it is crucial to know how CaMKII is localized to its postsynaptic targets because protein kinases often are targeted to their substrates by adapter proteins. Here we show that CaMKII directly binds to the NMDA receptor subunits NR1 and NR2B. Moreover, activation of CaMKIIalpha by stimulation of NMDA receptors in forebrain slices increase this association. This interaction places CaMKII not only proximal to a major source of Ca2+ influx but also close to alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA)-type glutamate receptors, which become phosphorylated upon stimulation of NMDA receptors in these forebrain slices. Identification of the postsynaptic adapter for CaMKII fills a critical gap in the understanding of LTP because CaMKII-mediated phosphorylation of AMPA receptors is an important step during LTP.  (+info)

Ca2+/calmodulin-kinase II enhances channel conductance of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate type glutamate receptors. (5/1822)

The ability of central glutamatergic synapses to change their strength in response to the intensity of synaptic input, which occurs, for example, in long-term potentiation (LTP), is thought to provide a cellular basis for memory formation and learning. LTP in the CA1 field of the hippocampus requires activation of Ca2+/calmodulin-kinase II (CaM-KII), which phosphorylates Ser-831 in the GluR1 subunit of the alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate glutamate receptor (AMPA-R), and this activation/phosphorylation is thought to be a postsynaptic mechanism in LTP. In this study, we have identified a molecular mechanism by which CaM-KII potentiates AMPA-Rs. Coexpression in HEK-293 cells of activated CaM-KII with GluR1 did not affect the glutamate affinity of the receptor, the kinetics of desensitization and recovery, channel rectification, open probability, or gating. Single-channel recordings identified multiple conductance states for GluR1, and coexpression with CaM-KII or a mutation of Ser-831 to Asp increased the contribution of the higher conductance states. These results indicate that CaM-KII can mediate plasticity at glutamatergic synapses by increasing single-channel conductance of existing functional AMPA-Rs or by recruiting new high-conductance-state AMPA-Rs.  (+info)

Site-specific phosphorylation of synapsin I by Ca2+/calmodulin-dependent protein kinase II in pancreatic betaTC3 cells: synapsin I is not associated with insulin secretory granules. (6/1822)

Increasing evidence supports a physiological role of Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) in the secretion of insulin from the pancreatic beta-cell, but the precise sites of action are not known. A role of this enzyme in neuroexocytosis is implicated by its phosphorylation of a vesicle-associated protein, synapsin I. Because of emerging similarities to the neuron with respect to exocytotic mechanisms, the expression and phosphorylation of synapsin I in the beta-cell have been studied. Synapsin I expression in clonal mouse beta-cells (betaTC3) and primary rat islet beta-cells was initially confirmed by immunoblot analysis. By immunoprecipitation, in situ phosphorylation of synapsin I was induced in permeabilized betaTC3 cells within a Ca2+ concentration range shown to activate endogenous CaM kinase II under identical conditions. Proteolytic digests of these immunoprecipitates revealed that calcium primarily induced the increased phosphorylation of sites identified as CaM kinase II-specific and distinct from protein kinase A-specific sites. Immunofluorescence and immunogold electron microscopy verified synapsin I expression in betaTC3 cells and pancreatic slices but demonstrated little if any colocalization of synapsin I with insulin-containing dense core granules. Thus, although this study establishes that synapsin I is a substrate for CaM kinase II in the pancreatic beta-cell, this event appears not to be important for the mobilization of insulin granules.  (+info)

Current studies on a working model of CaM kinase II in hippocampal long-term potentiation and memory. (7/1822)

There have been recent advances in understanding the molecular basis of the long-term potentiation (LTP) of synaptic transmission in the hippocampus. This review documents current views on mechanisms underlying LTP induction, from activation of the NMDA receptor to stimulation of Ca2+/calmodulin-dependent protein kinase II (CaM kinase II). We will focus in particular on recent findings of how CaM kinase II encodes the frequency of synaptic usage and serves as a molecular memory switch at the synapse. Furthermore, a role for CaM kinase II in spatial learning and memory is demonstrated by recent studies using transgenic mice.  (+info)

Differential effects of a calcineurin inhibitor on glutamate-induced phosphorylation of Ca2+/calmodulin-dependent protein kinases in cultured rat hippocampal neurons. (8/1822)

Calcium/calmodulin-dependent protein kinases (CaM kinases) are major multifunctional enzymes that play important roles in calcium-mediated signal transduction. To characterize their regulatory mechanisms in neurons, we compared glutamate-induced phosphorylation of CaM kinase IV and CaM kinase II in cultured rat hippocampal neurons. We observed that dephosphorylation of these kinases followed different time courses, suggesting different regulatory mechanisms for each kinase. Okadaic acid, an inhibitor of protein phosphatase (PP) 1 and PP2A, increased the phosphorylation of both kinases. In contrast, cyclosporin A, an inhibitor of calcineurin, showed different effects: the phosphorylation and activity of CaM kinase IV were significantly increased with this inhibitor, but those of CaM kinase II were not significantly increased. Cyclosporin A treatment of neurons increased phosphorylation of Thr196 of CaM kinase IV, the activated form with CaM kinase kinase, which was recognized with an anti-phospho-Thr196 antibody. Moreover, recombinant CaM kinase IV was dephosphorylated and inactivated with calcineurin as well as with PP1, PP2A, and PP2C in vitro. These results suggest that CaM kinase IV, but not CaM kinase II, is directly regulated with calcineurin.  (+info)

Olomoucine is known as a cyclin-dependent kinase inhibitor. We found that olomoucine blocked insulins ability to stimulate glucose transport. It did so without affecting the activity of known insulin signaling proteins. To identify the olomoucine-sensitive kinase(s), we prepared analogs that could be immobilized to an affinity resin to isolate binding proteins. One of the generated analogs inhibited insulin-stimulated glucose uptake with increased sensitivity compared with olomoucine. The IC50 for inhibition of insulin-stimulated glucose uptake occurred at analog concentrations as low as 0.1 μm. To identify proteins binding to the analog, [35S]-labeled cell lysates prepared from 3T3-L1 adipocytes were incubated with analog chemically cross-linked to a resin support and binding proteins analyzed by SDS-PAGE. The major binding species was a doublet at 50-60 kDa, which was identified as calcium/calmodulin-dependent protein kinase II (CaMKII) by N-terminal peptide analysis and confirmed by ...
Active calcium/calmodulin-dependent protein kinase II (CaMKII) has been reported to take a critical role in the induction of long-term potentiation (LTP). Changes in CaMKII activity were detected in various ischemia models. It is tempting to know whether and how CaMKII takes a role in NMDA receptor (NMDAR)-mediated postischemic long-term potentiation (NMDA i-LTP). Here, we monitored changes in NMDAR-mediated field excitatory postsynaptic potentials (NMDA fEPSPs) at different time points following ischemia onset |i|in vitro|/i| oxygen and glucose deprivation (OGD) ischemia model. We found that 10 min OGD treatment induced significant i-LTP in NMDA fEPSPs, whereas shorter (3 min) or longer (25 min) OGD treatment failed to induce prominent NMDA i-LTP. CaMKII activity or CaMKII autophosphorylation displays a similar bifurcated trend at different time points following onset of ischemia both |i|in vitro|/i| OGD or |i|in vivo|/i| photothrombotic lesion (PT) models, suggesting a correlation
TY - JOUR. T1 - Mutational analysis of Ca2+-independent autophosphorylation of calcium/calmodulin-dependent protein kinase II. AU - Mukherji, Sucheta. AU - Soderling, Thomas. PY - 1995/6/9. Y1 - 1995/6/9. N2 - Previous studies with synthetic peptides indicate that residues 290-309, corresponding to the calmodulin (CaM)-binding domain of Ca2+/CaM-dependent protein kinase II interact with the catalytic core of the enzyme as a pseudosubstrate (Colbran, R. J., Smith, M. K., Schworer, C. M., Fong, Y. L., and Soderling, T. R. (1989) J. Biol. Chem. 264, 4800-4804). In the present study, we attempted to locate the pseudosubstrate motif by generation or removal of potential substrate recognition sequences (RXXS/T) at selected positions using site-directed mutagenesis. Based on previous results, Arg297, Thr305/306, and Ser314 were selected as key residues. Single mutations such as N294S, K300S, A302R, A309R, and R311A were expressed, purified, and characterized. Several of the mutants exhibited decreased ...
Calcium/calmodulin-dependent protein kinase IIα (CamKIIα) may modulate the function of mu-opioid receptors by phosphorylation and therefore, be involved in development of morphine-induced analgesic tolerance.The current study aimed to examine changes in gene expression of CamKIIα in the lumbosacral cord and midbrain during induction of morphine analgesic tolerance.Male Wistar rats weighing 250 - 300 g were used. Two groups of rats (n = 6 per group) received saline (1 mL/kg) or morphine (10 mg/mL/kg) twice-daily for eight days, and induction of morphine analgesic tolerance was assessed using a hotplate test on days one, four and eight of the injections. The lumbosacral spinal cord and midbrain were also dissected in six independent groups (n = 4 per group) on days one, four and eight of saline or morphine injections to examine changes in gene expression of CamKIIα with a semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) method.The result of the hotplate test showed that the
This study investigates the roles of hippocampal N-methyl-D-aspartate (NMDA) glutamate receptors and CaMKII (calcium/calmodulin-dependent protein kinase II) in amphetamine-produced conditioned place preference (AMPH-CPP) in rats. An earlier report showed that AMPH-CPP resulted in the enhancement of …
TY - JOUR. T1 - CaM Kinase II-dependent pathophysiological signalling in endothelial cells. AU - Cai, Hua. AU - Liu, Depei. AU - Garcia, Joe G.N.. N1 - Funding Information: The authors work are supported by National Heart, Lung, and Blood Institute (NHLBI) Grants HL-077440 (H.L.C.), HL-081571 (H.L.C.), HL057244 (Pin-Lan Li and H.L.C.), an American Heart Association Grant 0435189N (H.L.C.), an American Diabetes Association Award 7-04-RA-16 (H.L.C.), and NHLBI PPG HL 58064 (J.G.N.G.).. PY - 2008/1. Y1 - 2008/1. N2 - Calcium/calmodulin-dependent protein kinase II (CaM Kinase II) is a known modulator of cardiac pathophysiology. The present review uniquely focuses on novel CaM Kinase II-mediated endothelial cell signalling which, under pathophysiological conditions, may indirectly modulate cardiac functions via alterations in endothelial or endocardial responses. CaM Kinase II has four different isoforms and various splicing variants for each isoform. The endothelial cell CaM Kinase II isoforms are ...
TY - JOUR. T1 - Identification of a Ca2+/calmodulin-dependent protein kinase II regulatory phosphorylation site in non-N-methyl-D-aspartate glutamate receptors. AU - Yakel, Jerrel L.. AU - Vissavajjhala, Prabhakar. AU - Derkach, Victor A.. AU - Brickey, Debra A.. AU - Soderling, Thomas R.. PY - 1995/2/28. Y1 - 1995/2/28. N2 - Glutamate receptor ion channels are colocalized in postsynaptic densities with Ca2+/calmodulin-dependent protein kinase II (CaM-kinase II), which can phosphorylate and strongly enhance non-N-methyl-D-aspartate (NMDA) glutamate receptor current. In this study, CaM-kinase II enhanced kainate currents of expressed glutamate receptor 6 in 293 cells and of wild-type glutamate receptor 1, but not the Ser-627 to Ala mutant, in Xenopus oocytes. A synthetic peptide corresponding to residues 620-638 in GluR1 was phosphorylated in vitro by CaM-kinase II but not by cAMP-dependent protein kinase or protein kinase C. The 32P-labeled peptide map of this synthetic peptide appears to be the ...
TY - JOUR. T1 - Ca2+/calmodulin-dependent protein kinase II regulates Tiam1 by reversible protein phosphorylation. AU - Fleming, I N. AU - Elliott, C M. AU - Buchanan, F G. AU - Downes, C P. AU - Exton, J H. PY - 1999. Y1 - 1999. N2 - A number of guanine nucleotide exchange factors have been identified that activate Rho family GTPases, by promoting the binding of GTP to these proteins. We have recently demonstrated that lysophosphatidic acid and several other agonists stimulate phosphorylation of the Rac1-specific exchange factor Tiam1 in Swiss 3T3 fibroblasts, and that protein kinase C is involved in Tiam1 phosphorylation (Fleming, I. N., Elliott, C. M., Collard, J. G., and Exton, J. H. (1997) J. Biol. Chem. 272, 33105-33110). We now show, through manipulation of intracellular [Ca2+] and the use of protein kinase inhibitors, that both protein kinase Calpha and Ca2+/calmodulin-dependent protein kinase II are involved in the phosphorylation of Tiam1 in vivo. Furthermore, we show that ...
article{dd19855c-025e-4a5e-9215-43f4f9e9a4a1, abstract = {The NMDA receptor complex represents a key molecular element in the pathogenesis of long-term synaptic changes and motor abnormalities in Parkinsons disease (PD). Here we show that NMDA receptor 1 (NR1) subunit and postsynaptic density (PSD)-95 protein levels are selectively reduced in the PSD of dopamine (DA)-denervated striata. These effects are accompanied by an increase in striatal levels of alphaCa(2+)-calmodulin-dependent protein kinase II (alphaCaMKII) autophosphorylation, along with a higher recruitment of activated alphaCaMKII to the regulatory NMDA receptor NR2A-NR2B subunits. Acute treatment of striatal slices with R(+)-7-chloro-8-hydroxy-3-methyl-1-phenyl-2,3,4,5-tetrahydro-1H-3-benzaz epine hydrochloride, but not with L-sulpiride, mimicked the effect of DA denervation on both alphaCaMKII autophosphorylation and corticostriatal synaptic plasticity. In addition to normalizing alphaCaMKII autophosphorylation levels as well as ...
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TY - JOUR. T1 - Amphetamine activate protein kinase C and calcium/calmodulin-dependent protein kinase via NMDA receptor in primary cultures of rat cortical neurons. AU - Wu, Hsueh-Hsia. AU - Lee, Horng-Mo. PY - 1999. Y1 - 1999. M3 - Article. VL - 1. SP - 12. EP - 19. JO - New Taipei Journal of Medicine. JF - New Taipei Journal of Medicine. SN - 1562-4242. ER - ...
Authors Affiliations: 1Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Istituto di Endocrinologia e Oncologia Sperimentale del CNR Naples; 2Department of Neuroscience, Reproductive and Odontostomatological Sciences, University of Naples Federico II, Naples; 3Department of Medicine-DIMED, Unit of Endocrinology, University of Padua, Padova; and 4Department of Medicine and Surgery, University of Salerno, Salerno, ...
We investigated the effects of Wenxin Keli (WXKL) on the Calcium/Calmodulin dependent kinase II (CaMK II) signal transduction pathway with transverse aortic constriction (TAC) rats. Echocardiographic measurements were obtained 3 and 9 weeks after the surgery. Meanwhile, the action potentials (APDs) were recorded using the whole-cell patch clamp technique, and western blotting was used to assess components of the CaMK II signal transduction pathway. At both 3 and 9 weeks after treatment, the fractional shortening (FS%) increased in the WXKL group compared with the TAC group. The APD|sub|90|/sub| of the TAC group was longer than that of the Sham group and was markedly shortened by WXKL treatment. Western blotting results showed that the protein expressions of CaMK II, phospholamban (PLB), and ryanodine receptor 2 (RYR2) were not statistically significant among the different groups at both treatment time points. However, WXKL treatment decreased the protein level and phosphorylation of CaMK II (Thr
Secretoneurin, a Novel Endogenous CaMKII Inhibitor, Augments Cardiomyocyte Calcium Handling and Inhibits Arrhythmogenic Calcium ...
There are comments on PubPeer for publication: Ca(2+)/calmodulin-dependent protein kinase II promotes cell cycle progression by directly activating MEK1 and subsequently modulating p27 phosphorylation (2009)
Greiser and colleagues (10) also report that, consistent with previous studies, the remaining RyR2 clusters were hyperphosphorylated at the protein kinase A (PKA) phosphorylation site (Ser2808), which may compensate for the reduction in RyR2 protein expression and help sustain subsarcolemmal Ca2+ release despite reduced L-type Ca2+ currents (Figure 1B). However, RAP myocytes exhibited reduced RyR2 phosphorylation at the calmodulin-dependent protein kinase II (CaMKII) phosphorylation site (Ser2815) and no changes in CaMKII activity. This finding contrasts with previous studies that reported increased atrial CaMKII activity and CaMKII-dependent RyR2-Ser2815 phosphorylation in human AF (5). Moreover, other studies have shown that treatment with CaMKII inhibitors or selective disruption of the Ser2815 CaMKII phosphorylation site prevented AF in animal models through a reduction of SR Ca2+ leak (12). One explanation for this discrepancy could be the limited duration of pacing in the rabbit model used ...
TY - JOUR. T1 - Persistent reversal of enhanced amphetamine intake by transient CaMKII inhibition. AU - Loweth, Jessica A.. AU - Li, Dongdong. AU - Cortright, James J.. AU - Wilke, Georgia. AU - Jeyifous, Okunola. AU - Neve, Rachael L.. AU - Bayer, K. Ulrich. AU - Vezina, Paul. PY - 2013/1/23. Y1 - 2013/1/23. N2 - Amphetamine exposure transiently increases Ca2+/calmodulin-dependent protein kinase II (CaMKII) α expression in the nucleus accumbens (NAcc) shell and this persistently increases local GluA1 S831 phosphorylation and enhances behavioral responding to the drug. Here we assessed whether transiently interfering with CaMKII signaling using a dominant-negative CaMKIIα mutant delivered to the NAcc shell with herpes simplex viral vectors could reverse these long-lasting biochemical and behavioral effects observed following exposure to amphetamine. As expected, transient expression of CaMKIIα K42M in the NAcc shell produced a corresponding transient increase in CaMKIIα and decrease in ...
TY - JOUR. T1 - β-Adrenergic induced SR Ca2 + leak is mediated by an Epac-NOS pathway. AU - Pereira, Laëtitia. AU - Bare, Dan J.. AU - Galice, Samuel. AU - Shannon, Thomas R.. AU - Bers, Donald M. PY - 2017/7/1. Y1 - 2017/7/1. N2 - Cardiac β-adrenergic receptors (β-AR) and Ca2 +-Calmodulin dependent protein kinase (CaMKII) regulate both physiological and pathophysiological Ca2 + signaling. Elevated diastolic Ca2 + leak from the sarcoplasmic reticulum (SR) contributes to contractile dysfunction in heart failure and to arrhythmogenesis. β-AR activation is known to increase SR Ca2 + leak via CaMKII-dependent phosphorylation of the ryanodine receptor. Two independent and reportedly parallel pathways have been implicated in this β-AR-CaMKII cascade, one involving exchange protein directly activated by cAMP (Epac2) and another involving nitric oxide synthase 1 (NOS1). Here we tested whether Epac and NOS function in a single series pathway to increase β-AR induced and CaMKII-dependent SR Ca2 + ...
Nuclear factor-kappa beta (NF-kβ) pro-inflammatory signalling is important in modulating endothelial dysfunction and may be important in vascular dysfunction associated with the ageing process. Recent studies in the heart have highlighted Calcium/calmodulin-dependent protein kinase II (CaMKII) as a novel regulator of NF-kβ signalling. However nothing is known of the role this interaction could play in regulating dysfunction of the vasculature during ageing. Here we (i) characterise NF-kβ signalling in vascular endothelial cells and examine the potential for CaMKII modulation and (ii) determine whether CaMKIIδ expression is altered in ageing.. Using human umbilical vein endothelial cells (HUVECs) as an in vitro model system, initial experiments have established that pro-inflammatory NF-kB signalling is activated in response to both tumour necrosis factor α (TNF-α) and interleukin-1β (IL-1β) stimulation. This was shown by a significant reduction in IkBα expression (1.18 ± 0.16 vs. 0.48 ...
Three genes encoding different Ca2+/calmodulin-dependent protein kinases have been characterized in the wheat phytopathogenic fungus Stagonospora nodorum. The kinases were identified from the S. nodorum genome sequence on the basis of sequence homology to known Ca2+/calmodulin- dependent protein kinases. Expression analysis determined that each of the kinases was expressed during growth in vitro and also during infection. The onset of sporulation triggered increased transcript levels of each of the kinases, particularly CpkA where an 11-fold increase in expression was observed during sporulation in planta. The role of the kinases was further determined via a reverse genetics approach. The disruption of CpkA affected vegetative growth in vitro and also sporulation. The cpkA strains produced 20-fold less spores on complex media and were unable to sporulate on defined minimal media. Infection assays showed that CpkA was not required for lesion development but was essential for sporulation at the ...
Rationale:: In the failing heart, persistent β-adrenergic receptor (βAR) activation is thought to induce myocyte death by protein kinase A (PKA)-dependent and PKA-independent activation of calcium/calmodulin-dependent kinase II (CaMKII) β-Adrenergic signaling pathways are also capable of activating cardioprotective mechanisms. Objective: This study used a novel PKA inhibitor peptide (PKI) to inhibit PKA activity to test the hypothesis that βAR signaling causes cell death through PKA-dependent pathways and cardioprotection through PKA-independent pathways. Methods and Results: In PKI transgenic mice, chronic isoproterenol (ISO) failed to induce cardiac hypertrophy, fibrosis, myocyte apoptosis and depressed cardiac function. In cultured adult feline ventricular myocytes (AFVMs), PKA inhibition protected myocytes from death induced byβ1-AR agonists by preventing cytosolic and SR Ca2+ overload and CaMKII activation. PKA inhibition revealed a cardioprotective role of β-adrenergic signaling via ...
Studies over the past decade have demonstrated that SR Ca2+ release is abnormal in patients with chronic AF.2 Whereas the amplitude of the L-type Ca2+ current is generally decreased in AF, Ca2+ leak through RyR2 is typically elevated despite similar or decreased SR Ca2+ contents.2,5 It has been proposed that triggered activity due to DADs is caused by an inward depolarizing INCX current, which occurs in response to the removal of excess Ca2+ release from the cytosol.9,10 We provide direct experimental evidence for this mechanism in the FKBP12.6−/− mouse model of AF. Our data revealed that FKBP12.6−/− mice exhibit atrial focal activity and AF caused by SR Ca2+ leak, NCX activation, and DADs generation. Because recent studies revealed that CaMKII phosphorylation of RyR2 at S2814 is elevated in patients with chronic AF,4,5 we investigated whether inhibition of S2814 phosphorylation of RyR2 affected susceptibility of FKBP12.6−/− to AF. Our results demonstrate that inhibition of S2814 but ...
TY - JOUR. T1 - CaMKII in myocardial hypertrophy and heart failure. AU - Anderson, Mark E.. AU - Brown, Joan Heller. AU - Bers, Donald M. PY - 2011/10. Y1 - 2011/10. N2 - Many signals have risen and fallen in the tide of investigation into mechanisms of myocardial hypertrophy and heart failure (HF). In our opinion, the multifunctional Ca and calmodulin-dependent protein kinase II (CaMKII) has emerged as a molecule to watch, in part because a solid body of accumulated data essentially satisfy Kochs postulates, showing that the CaMKII pathway is a core mechanism for promoting myocardial hypertrophy and heart failure. Multiple groups have now confirmed the following: (1) that CaMKII activity is increased in hypertrophied and failing myocardium from animal models and patients; (2) CaMKII overexpression causes myocardial hypertrophy and HF and (3) CaMKII inhibition (by drugs, inhibitory peptides and gene deletion) improves myocardial hypertrophy and HF. Patients with myocardial disease die in equal ...
Author Summary Memory is understood as strengthened synaptic connections among neurons. Paradoxically components of synaptic membranes are relatively short-lived and frequently re-cycled while memories can last a lifetime. This suggests synaptic information is encoded at a deeper, finer-grained scale of molecular information within post-synaptic neurons. Long-term memory requires genetic expression, protein synthesis, and delivery of new synaptic components. How are these changes guided on the molecular level? The calcium-calmodulin dependent protein kinase II (CaMKII) has been heavily implicated in the strengthening of active neural connections. CaMKII interacts with various substrates including microtubules (MTs). MTs maintain cellular structure, and facilitate cellular cargo transport, effectively controlling neural architecture. Memory formation requires reorientation of this network. Could CaMKII-MT interactions be the molecular level encoding required to orchestrate neural plasticity? Using
CAMK2A - CAMK2A (Myc-DDK-tagged)-Human calcium/calmodulin-dependent protein kinase II alpha (CAMK2A), transcript variant 2 available for purchase from OriGene - Your Gene Company.
CaMKII alpha antibody (calcium/calmodulin-dependent protein kinase II alpha) for ICC/IF, IHC-Fr, WB. Anti-CaMKII alpha pAb (GTX127939) is tested in Mouse, Rat samples. 100% Ab-Assurance.
Objectives:Sepsis is associated with cardiac contractile dysfunction attributed to alterations in Ca2+ handling. We examined the subcellular mechanisms involved in sarcoplasmic reticulum Ca2+ loss that mediate altered Ca2+ handling and contractile dysfunction associated with sepsis.Design:Randomized
Mouse eggs are ovulated following arrest at metaphase of the second meiotic division (metII). Fertilization breaks this arrest, with the egg extruding a second polar body (PB2) and forming pronuclei. Ca2+ spikes induced by phospholipase C zeta, which are introduced into the egg on gamete fusion, are responsible for causing the degradation of Erp1/Emi2 (Fbxo43 - Mouse Genome Informatics) (Ducibella and Fissore, 2008; Jones, 2005; Mehlmann, 2005; Swann et al., 2006). Erp1/Emi2 loss activates the anaphase-promoting complex (APC) and so drives exit from meiosis (Madgwick et al., 2006; Shoji et al., 2006).. In frog eggs, the Ca2+ fertilization signal switches on calmodulin-dependent protein kinase II (CamKII; Camk2), which phosphorylates Erp1/Emi2 and so promotes its degradation (Liu and Maller, 2005; Rauh et al., 2005; Schmidt et al., 2005). Consistent with this more recent development in the understanding of the molecular events of activation, it had been discovered several years previously, also ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Introduction: Glutamate excitotoxicity is an important mechanism of ischemic neuronal damage, however inhibition of glutamate receptors has proven an unsuccessful strategy. Ca2+/calmodulin-dependent protein kinase II (CaMKII) is a major downstream target of glutamate signaling. The aim of this study is to examine the neuroprotective potential of inhibition of autonomous CaMKII activity using novel peptide inhibitors (tatCN21,19o) and transgenic mice.. Methods: C57BL/6 male wild-type (WT) and T286A mutant mice were subjected to 6 min of cardiac arrest and CPR. Mice were randomized to tatCN21 (1 mg/kg), tatCN19o (0.01,0.1,1 mg/kg) or control (tatSCR; 1 mg/kg), administered 30 min after CPR (iv). Separate experiments were performed to assess mild post-arrest hypothermia (rectal T = 34 ±0.2 °C for 1 hr after CPR). Hippocampal neuronal damage was analyzed 3 days after CA/CPR by H&E staining. Total CaMKII and Thr-286 phosphorylation levels were measured by western blot. Statistical analyses were ...
CAMK2B - CAMK2B (Myc-DDK-tagged)-Human calcium/calmodulin-dependent protein kinase II beta (CAMK2B), transcript variant 7 available for purchase from OriGene - Your Gene Company.
Overview Protocols Specifications Resources Convenient, 1-Hour Kinase Activity Assays The SignaTECT® Protein Kinase Assay Systems provide a rapid, radioactivity-based method for detection of kinase activity. The method is based on capture of phosphorylated labeled substrates on the SAM2® Biotin Capture Membrane, which
Anti-peptide antibodies specific for the neuronal calcium channel alpha 1E subunit (anti-CNE1 and anti-CNE2) were produced to study the biochemical properties and subcellular distribution of the alpha 1E polypeptide from rat brain. Immunoblotting identified a single size form of 245-255 kDa which was a substrate for phosphorylation by cAMP- dependent protein kinase, protein kinase C, cGMP-dependent protein kinase, and calcium/calmodulin-dependent protein kinase II. Ligand- binding studies of alpha 1E indicate that it is not a high affinity receptor for the dihydropyridine isradipine or the peptide toxins omega- conotoxin GVIA or omega-conotoxin MVIIC at concentrations which elicit high affinity binding to other channel types in the same membrane preparation. The alpha 1E subunit is widely distributed in the brain with the most prominent immunocytochemical staining in deep midline structures such as caudate-putamen, thalamus, hypothalamus, amygdala, cerebellum, and a variety of nuclei in the ...
Since its inception, the synaptic tagging hypothesis has inspired many to search for synaptic tags. However, very few molecules have been proposed as candidates (Frey and Frey, 2008). The nature and identity of PRPs and synaptic tags are under intensive investigations (Frey and Frey, 2008). Two pathway experiments showed that blockade of protein kinase A (PKA) or its interaction with A kinase-anchoring proteins (AKAPs) prevents synaptic capture, suggesting that PKA or its anchoring at active synapses may serve as a synaptic tag for L-LTP (Huang et al., 2006; Young et al., 2006). Calcium/calmodulin-dependent protein kinase II (CaMKII) is also implicated as an L-LTP-specific tag (Sajikumar et al., 2007). Studies thus far have been based on the use of pharmacological inhibitors in two-pathway experiments. However, it is necessary to show that these tags are transiently and locally activated in a protein synthesis-independent manner by weak stimulation. In a recent study, NMDA-dependent, ...
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The Ca2+/calmodulin-dependent protein kinase II (CaMKII) is a major mediator of long-term potentiation (LTP) and depression (LTD), two opposing forms of synaptic plasticity underlying learning, memory and cognition. The heterozygous CaMKIIα isoform KO (CaMKIIα+/-) mice have a schizophrenia-related phenotype, including impaired working memory. Here, we examined synaptic strength and plasticity in two brain areas implicated in working memory, hippocampus CA1 and medial prefrontal cortex (mPFC). Young CaMKIIα+/- mice (postnatal days 12-16; corresponding to a developmental stage well before schizophrenia manifestation in humans) showed impaired hippocampal CA1 LTP. However, this LTP impairment normalized over development and was no longer detected in older CaMKIIα+/- mice (postnatal weeks 9-11; corresponding to young adults). By contrast, the CaMKIIα+/- mice failed to show the developmental increase of basal synaptic transmission in the CA1 seen in wild-type (WT) mice, resulting in impaired basal
Complete information for CAMK4 gene (Protein Coding), Calcium/Calmodulin Dependent Protein Kinase IV, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
The concept of subcellular targeting by anchoring proteins is of major importance for understanding the specificity of signal transduction. The work presented here constitutes the first description of an anchoring protein for multifunctional CaM kinase II. αKAP exhibits three properties expected of anchoring proteins. (i) It is restricted to a specific cellular compartment, it is membrane bound and probably directly inserted into SR membranes by its N‐terminal hydrophobic domain (Figures 3 and 4). (ii) It binds CaM kinase II. This binding occurs within intact cells and not during extraction of transfected cells, since significant interaction was only detected after coexpression of αKAP and CaM kinase II, but not when individually expressed proteins were mixed (Figure 6). (iii) It is responsible for the targeting of the novel βM‐CaM kinase II to the SR, since it co‐immunoprecipitates with kinase extracted from SR membranes and the kinase does not have the physical properties of a ...
Complete information for CAMKK2 gene (Protein Coding), Calcium/Calmodulin Dependent Protein Kinase Kinase 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Investigating the transduction of TGF-?-mediated Ca2+-signalling by Ca2+/calmodulin protein kinase II and its effects on extracellular matrix gene expression and cell viability in human pulmonary fibroblasts. ...
cDNA clones coding for the β subunit of rat brain type II Ca2+/calmodulin-dependent protein kinase were isolated and sequenced. The clones, including one containing the entire coding region, hybridize at high stringency to a single band of poly(A)+ RNA of length 4.8 kilobases. The subunit coded for by the clones was identified by in vitro transcription of the cDNA followed by translation of the resulting RNA. The DNA sequence of the clones contains a single long open reading frame (1626 nucleotides) coding for a protein of 542 amino acids with a molecular weight of 60,333, the amino-terminal half of which is homologous to several other protein kinases. Potential ATP- and calmodulin-binding domains were identified. Two independent clones contain an identical 45-nucleotide deletion, relative to the clones described above, resulting in a shorter open reading frame coding for a protein of molecular weight 58,000. This suggests that the minor, 58-kDa β subunit of the type II ...
Lys-Lys-Lys-Leu-Arg-Arg-Gln-Glu-Ala-Phe-Asp-Ala-Tyr4374-v 0.5 mg | 90.00 EUR[Lys3, Phe10, Tyr13]-Autocamtide-2-Related Inhibitory Peptide ...
calmodulin-dependent protein kinase V: widely distributed in various tissues, involved in calcium-regulated processes; from rat brain; may exist in 40 & 41 kDa isoforms; amino acid sequence has been determined
KN-92 is an inactive derivative of KN-93. KN-93 is a selective inhibitor of Ca2+/calmodulin-dependent kinase II (CaMKII), competitively blocking CaM binding to the kinase (Ki = 370 nM).
The major finding of this study is that GIT1 is a novel mediator of Ang II-mediated VSMC gene transcription. Specifically we show that GIT1 participates in an Ang II signaling pathway that involves phosphorylation of HDAC5 and activation of MEF2, downstream of a pathway that requires Src, PLCγ, and CamK II (supplemental Figure V). Our results suggest that GIT1, via its multi-domain scaffolding function, coordinates Ang II signaling events that control calcium-dependent signaling (PLCγ and CamK II).. The focus of the present study is on CamK II which is a family of cytosolic serine/threonine protein kinases that exist as multimers consisting of α, β, δ, or γ subunits, each encoded by a different gene.24,25 Whereas CamK IIα and β are mainly expressed in neuronal tissues, CaMKIIδb, CaMKIIδc, and CaMKIIγ are abundant in the heart8,21,26 and VSMCs.27 CaMKII can phosphorylate type II HDACs.11 These HDACs (HDAC 4, 5, 7, and 9) normally repress transcriptional activity (eg, activation driven ...
Control of cardiomyocyte cytosolic Ca(2+) levels is crucial in determining inotropic status and ischemia/reperfusion stress response. Responsive to fluctuations in cellular Ca(2+), Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) is a serine/threonine kinase integral to the processes regulating cardiomyocyte Ca(2+) channels/transporters. CaMKII is primarily expressed either in the δB or δC splice variant forms, which may mediate differential influences on cardiomyocyte function and pathological response mechanisms. Increases in myocyte Ca(2+) levels promote the binding of a Ca(2+)/calmodulin complex to CaMKII, to activate the kinase. Activity is also maintained through a series of post-translational modifications within a critical region of the regulatory domain of the protein. Recent data indicate that the post-translational modification status of CaMKIIδB/δC variants may have an important influence on reperfusion outcomes. This study provided the first evidence that the specific type ...
Wet-lab validated real-time PCR primer assays for your biological pathway of interest. Select your gene target of interest using an interactive pathway map, and select your plate.
Looking for online definition of CaM kinase II delta subunit in the Medical Dictionary? CaM kinase II delta subunit explanation free. What is CaM kinase II delta subunit? Meaning of CaM kinase II delta subunit medical term. What does CaM kinase II delta subunit mean?
Excessive activation of β-adrenergic, angiotensin II, and aldosterone signaling pathways promotes mortality after myocardial infarction (MI), while antagonist drugs targeting these pathways are core therapies for treating post-MI patients. The multifunctional calcium/calmodulin-dependent protein kinase II (CaMKII) is activated by catecholamines and angiotensin II, and CaMKII inhibition prevents isoproterenol- and angiotensin II-mediated cardiomyopathy. Here we ask the hypothesis if aldosterone and CaMKII participated in common responses to MI by developing a mouse MI model supplemented by aldosterone infusion (MI+Aldo) to approximate plasma aldosterone levels measured in MI patients. We find that aldosterone exerts direct toxic actions on myocardium by oxidative activation of CaMKII, causing cardiac rupture and increased mortality in mice after MI (65.5% for aldosterone versus 31.0% for vehicle, P=0.007, n≥19 mice per treatment). Aldosterone oxidizes CaMKII by recruiting NADPH oxidase, and ...
Our data show that LTP inducing stimulation produces a 6-fold increase in endogenous synapsin I phosphorylation at its CaM kinase II sites. The observed rise in synapsin I phosphorylation occurred rapidly after the stimulation and persisted for at least 30 min. Most importantly, this effect was not observed in the presence of the NMDA receptor antagonist APV, suggesting that the rise in synapsin I phosphorylation is due to the LTP-inducing nature of the stimulation. Since synapsin I is localized exclusively in the presynaptic terminal, our data provide strong evidence that LTP expression is accompanied by persistent biochemical changes in the presynaptic terminal. Because the observed LTP-associated presynaptic changes require NMDA receptor activation, these data support models of LTP in which a retrograde messenger (generated subsequent to postsynaptic NMDA receptor activation) may produce presynaptic changes underlying LTP (36-38).. The mechanism by which LTP induction might lead to this ...
TY - JOUR. T1 - CASK and CaMKII function in the mushroom body α/ß neurons during Drosophila memory formation. T2 - CASK and CaMKII in memory. AU - Malik, Bilal R. AU - Gillespie, John M. AU - Hodge, James J L. PY - 2013/3/27. Y1 - 2013/3/27. N2 - CaMKII is a central molecule in mechanisms of synaptic plasticity and memory. A vital feature of CaMKII in plasticity is its ability to switch to a calcium (Ca2+) independent constitutively active state after autophosphorylation at threonine 287 (T287). A second pair of sites, T306 T307 in the calmodulin (CaM) binding region once autophosphorylated, prevent subsequent CaM binding and inactivates the kinase during synaptic plasticity and memory. Recently a synaptic molecule called CASK has been shown to control both sets of CaMKII autophosphorylation events and hence is well poised to be a key regulator of memory. We show deletion of full length CASK or just its CaMK-like and L27 domains disrupts middle-term memory (MTM) and long-term memory (LTM), ...
Introduction: The calcium/calmodulin-dependent kinase II (CaMKII) is activated by angiotensin-II, a strong inducer of vascular smooth muscle cell (VSM) hypertrophy. CaMKII activates HDAC4/MEF-2 dependent gene transcription by phosphorylation of HDAC4 S467 and 632. Here, we demonstrate that CaMKII mediates Ang-II-induced VSM hypertrophy in vitro and in vivo by activation of the HDAC4/MEF-2 signal transduction pathway.. Methods and Results: Medial hypertrophy by Ang-II infusion at pressor dose over 10 days was significantly reduced in C57Bl/6 mice when the CaMKII inhibitor KN93 was given daily i.p. (0.070 mm2 vs 0.052 mm2, p,0.05). In vitro, Ang-II increased the 3H-Leucine/3H-Thymidine uptake in control aortic VSM cells by 50% after 24 hr, whereas overexpression of the CaMKII peptide inhibitor CaMKIIN resulted only in 14 % increase (p,0.05). Ang-II induced phosphorylation of HDAC4 that was further increased under overexpression of CaMKIIδ2. CaMKII overexpression resulted in increased ...
Raval A, Tanner SM, Byrd JC, Angerman EB, Perko JD, Chen SS, Hackanson B, Grever MR, Lucas DM, Matkovic JJ, Lin TS, Kipps TJ, Murray F, Weisenburger D, Sanger W, Lynch J, Watson P, Jansen M, Yoshinaga Y, Rosenquist R, de Jong PJ, Coggill P, Beck S, Lynch H, de la Chapelle A, Plass C. Downregulation of death-associated protein kinase 1 (DAPK1) in chronic lymphocytic leukemia. Cell. 2007 Jun 01; 129(5):879-90 ...
Bidirectional changes in the efficacy of neuronal synaptic transmission, such as hippocampal long-term potentiation (LTP) and long-term depression (LTD), are thought to be mechanisms for information storage in the brain. LTP and LTD may be mediated by the modulation of AMPA (α-amino-3-hydroxy-5-methyl-4-isoxazloe proprionic acid) receptor phosphorylation. Here we show that LTP and LTD reversibly modify the phosphorylation of the AMPA receptor GluR1 subunit. However, contrary to the hypothesis that LTP and LTD are the functional inverse of each other, we find that they are associated with phosphorylation and dephosphorylation, respectively, of distinct GluR1 phosphorylation sites. Moreover, the site modulated depends on the stimulation history of the synapse. LTD induction in naive synapses dephosphorylates the major cyclic-AMP-dependent protein kinase (PKA) site, whereas in potentiated synapses the major calcium/calmodulin-dependent protein kinase II (CaMKII) site is dephosphorylated. Conversely, LTP
Small molecule inhibitors of the human sirtuins and calmodulin-dependent protein kinases have shown promising anti-cancer activity in cell-based screens and animal models. We have synthesized analogues of these compounds, identifying more selective sirtuin inhibitors and more potent calmodulin-dependent protein kinase inhibitors.The sirtuins are a family of NAD+-dependent deacetylases that regulate cellular aging and gene silencing in simple organisms and appear to play important regulatory roles in human cells that make them attractive anti-cancer targets. We have previously identified the compound cambinol, an inhibitor of the human sirtuins SIRT1 and SIRT2, which is selectively toxic to Burkitts lymphoma cells. In order to determine which sirtuin is the relevant target, we screened analogues of cambinol, identifying compounds that exhibited moderate selectivity for both SIRT1 and SIRT2. The compound JP136 is ten-fold more selective in vitro for SIRT1 over SIRT2, with respective IC50s of 13 ...
The abundantly expressed calcium/calmodulin-dependent protein kinase II (CAMK2), alpha (CAMK2A), and beta (CAMK2B) isoforms are essential for learning and memory formation. Recently, a de novo candidate mutation (p.Arg292Pro) in the gamma isoform of CAMK2 (CAMK2G) was identified in a patient with severe intellectual disability (ID), but the mechanism(s) by which this mutation causes ID is unknown. Here, we identified a second, unrelated individual, with a de novo CAMK2G p.Arg292Pro mutation, and used in vivo and in vitro assays to assess the impact of this mutation on CAMK2G and neuronal function. We found that knockdown of CAMK2G results in inappropriate precocious neuronal maturation. We further found that the CAMK2G p.Arg292Pro mutation acts as a highly pathogenic gain-of-function mutation, leading to increased phosphotransferase activity and impaired neuronal maturation as well as impaired targeting of the nuclear CAMK2G isoform. Silencing the catalytic site of the CAMK2G p.Arg292Pro protein ...
Looking for molecular memory? Find out information about molecular memory. A potential future memory technology that stores data at the molecular level. Using lasers and the bacteriorhodopsin protein molecule, the W. M. Explanation of molecular memory
This invention relates to the controlled release of calcium sulfate as well as to the controlled release of an additive to a calcium sulfate matrix such as medicaments or pesticides. The controlled release is achieved by a pellet comprising calcium sulfate. The pellet is prepared by the process comprising mixing powder consisting essentially of alpha-calcium sulfate hemihydrate, a solution comprising water, and, optionally, an additive and a powder consisting essentially of beta-calcium sulfate hemihydrate to form a mixture, and forming said mixture into a pellet, wherein said alpha-calcium sulfate hemihydrate and beta-calcium sulfate hemihydrate powders have specified properties such as BET surface areas, densities, mean particle sizes, and purifies. The dissolution rate of the calcium sulfate pellet is controlled by varying the weight ratio of the beta-calcium sulfate hemihydrate powder to the alpha-calcium sulfate hemihydrate powder from 0 to about 3. The invention also provides a method of
The Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) is critically required for the synaptic recruitment of AMPA-type glutamate receptors (AMPARs) during both development and plasticity. However, the underlying mechanism is unknown. Using single-particle tracking of AMPARs, we show that CaMKII …
0071] The peptide hormone Ang II induces opening of mitochondrial KATP channels, depolarizes ΔΨm, and amplifies ROS generation from mitochondria [46]. We have shown that inhibition of CaMKII can protect against Ang II induced ROS species generation (FIG. 6). H9C2 cells and eventually neonatal cardiomyocytes from WT and mitoCaMKIIN mice will be cultured as described above, and peptide-loaded PLGA/PAMAM nanoparticles with and without surface bound MLS ligands will be prepared as described above. Cells will be treated with Ang II (1 μM for 3 hours). Both H9C2 cells and neonatal cardiomyocytes will be tested. We plan to isolate cardiomyocytes from four mouse litters of mitoCaMKIIN mice. Because these transgenic mice are crossed to WT-C57 mice each generation, the WT littermates will be used to test treated and untreated nanoparticles. A series of increasing doses of nanoparticles will be co-incubated with Ang II to determine the dose-response curve for generating protection against changes ...
Multiple calmodulin (CaM) isoforms are expressed in plants, but their biochemical characteristics are not well resolved. Here we show the differential regulation exhibited by two soya bean CaM isoforms (SCaM-1 and SCaM-4) for the activation of five CaM-dependent enzymes, and the Ca2+ dependence of their target enzyme activation. SCaM-1 activated myosin light-chain kinase as effectively as brain CaM (Kact 1.8 and 1.7nM respectively), but SCaM-4 produced no activation of this enzyme. Both CaM isoforms supported near maximal activation of CaM-dependent protein kinase II (CaM KII), but SCaM-4 exhibited approx.12-fold higher Kact than SCaM-1 for CaM KII phosphorylation of caldesmon. The SCaM isoforms showed differential activation of plant and animal Ca2+-ATPases. The plant Ca2+-ATPase was activated maximally by both isoforms, while the erythrocyte Ca2+-ATPase was activated only by SCaM-1. Plant glutamate decarboxylase was activated fully by SCaM-1, but SCaM-4 exhibited an approx. 4-fold increase ...
Background: Hypertensive cardiomyopathy or pathophysiological changes in myocardial structure and function caused by hypertension is a growing clinical problem due to the ageing population and a lack of curative therapies. The onset of the disease is often clinically silent, progressing over time to therapy-resistant symptomatic forms. Existing therapeutic concepts are, therefore, symptom-oriented and tailored for advanced stages of cardiac remodeling. Understanding molecular processes driving early hypertension-induced changes may improve diagnosis and treatment options.. Recent evidence positions changes in Ca2+ cycling as an early promoter of cardiac remodeling via Ca2+-mediated regulation of transcription. The enzyme Ca2+/calmodulin-dependent protein kinase II (CaMKII) has a central role in this process, as it can translate fine changes in Ca2+ fluxes into altered gene expression. However, the specific regulation of this so-called excitation-transcription coupling in hypertensive ...
Heart failure stays a significant well being burden around the globe. Despite nice progress in delineation of molecular mechanisms underlying growth of illness, normal remedy has not superior on the similar tempo. The multifunctional signaling molecule Ca2+/calmodulin-dependent protein kinase II (CaMKII) has acquired appreciable consideration over current years for its central function in maladaptive reworking and arrhythmias … Read more. ...
Supplementary MaterialsSupplementary Fig 1 rsob180132supp1. and cells microarrays. The function of IL-38 on individual success prices After that, cancer development and their awareness to chemotherapy medications was assessed. IL-38 was barely expressed in the NSCLC tissue but expressed in the adjacent normal tissue highly. The downregulation of IL-38 was considerably correlated with the outcomes from the American Joint Committee on Cancers stage and PBDB-T amount of differentiation, and it had been also been shown to be an unbiased prognostic signal of disease-free success and overall success for sufferers PBDB-T with NSCLC. Overexpression of IL-38 in NSCLC cells suppressed cell migration, invasion, colony and proliferation development through suppressing -catenin. IL-38 inhibited NSCLC development within a mice model and sensitized the cancers cells to chemotherapy medications. Our results present that IL-38 performs an inhibitory function in NSCLC advancement and functions being a book ...
Viral infections affect three to five million patients annually. antiviral treatment, this review provides the verified data around the medicinal plants and related herbal substances ABT 492 meglumine (Delafloxacin meglumine) with antiviral activity, as well as applied strategies for the delivery of these herb extracts and biologically active phytochemicals. Open in a separate windows Graphical Abstract against most viruses, but often are ineffective in patients. Ninety different antiviral brokers available today [3, 4] only treat a selection of viruses; these viruses include HIV (human immunodeficiency computer virus), herpes viruses, including HSV (herpes simplex virus), hCMV (human cytomegalovirus), VZV (varicella zoster computer virus), influenza viruses, and the hepatitis viruses (Fig. ?(Fig.1).1). Currently, there is no approved remedy for many types or viruses, and vaccination is limited to hepatitis A computer virus, mumps, and varicella [2]. In addition, these brokers are often costly ...
We use Drosophila melanogaster for studying cardiac diseases. One transgenic strain containing a fluorescent reporter system that senses intracellular twitch Ca2+ transient increases was utilized. We observed changes in the frequency of Ca2+ transients with age in semi-intac fly heart preparations and its putative modification by 1) The inhibition of Ca2+-calmodulin-dependent protein kinase (CaMKII), involved in Ca2+ cycling and 2) the reduction in eIF4E expression, an eukaryotic translation initiation factor. Results: Flies between 60 and 70 days old showed reduction of heart rate compared with 7 days old flies and widespred distribution of cardiac periods (intervals between transient increases of Ca2+). Arrhythmicity index (standard deviation of the heart period), increased from 0.27 to 0.52 (n=12). CaMKII inhibition reduced dispersion of cardiac periods distribution and arrhythmicity index from 0.27 to 0.19 (n = 21). Lower levels of eIF4E reduced dispersion of cardiac periods distribution and ...
Our laboratory identified CaMKII as an important pro-arrhythmic and pro-cardiomyopathic signal. Our work has provided proof of concept evidence motivating active efforts in biotech and the pharmaceutical industry to develop therapeutic CaMKII inhibitory drugs to treat heart failure and arrhythmias. My group discovered that CaMKII is activated by oxidation and excessive CaMKII activity participates in cellular injury in response to diverse upstream signals, including the renin-angiotensin-aldosterone pathway and endotoxin by oxidative activation. CaMKII is multifunctional because it has multiple downstream targets. CaMKII catalyzed phosphorylation of diverse proteins in myocardium appears to coordinate activity of many or most voltage-gated ion channels, Ca2+ homeostatic proteins and gene transcription. Under physiological conditions, CaMKII is important for excitation-contraction coupling and fight or flight heart rate increases. However, myocardial CaMKII is excessively activated during ...
Structural and biophysical studies reveal how CaMKII kinases, which are important for cellular learning and memory, are switched on by binding of Ca2+/calmodulin.
Recombinant full-length human CAMK4 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CAMK4 is a multifunctional serine/threonine protein kinase and a member of Ca2+/calmodulin-dependent protein kinase family.
Roles of calmodulin and CaMKII in mediating PKG stimulation of Kir6.2/SUR2A channels.Recombinant Kir6.2/SUR2A channels were expressed in HEK293 cells by transie
There are no specific protocols for CaMKII alpha (phospho T286) peptide (ab115237). Please download our general protocols booklet
miR-185 negatively regulates the activity of CaMKIIδ in NRVMs.(A and B) 72 h after transfection of miR-185 mimic or miR-185 inhibitor, the level of p-CaMKIIδ
If you know of any papers that use this antibody, please contact us at antibodies [at] alzforum [dot] org for consideration in the References section.. ...
マウス・モノクローナル抗体 ab54925 交差種: Hu 適用: WB,IP,Flow Cyt,ICC/IF…CaMKII alpha抗体一覧…画像、プロトコール、文献などWeb上の情報が満載のアブカムの Antibody 製品。国内在庫と品質保証制度も充実。
"CAMK2A calcium/calmodulin dependent protein kinase II alpha [Homo sapiens (human)] - Gene - NCBI". www.ncbi.nlm.nih.gov. ... β CAMKIIδ CAMKIIγ CAMKIII CAMKIV CAMKV CaM kinase like vesicle associated SCAMK Ca2+/calmodulin-dependent protein kinase kinase ... CAMK, also written as CaMK, is an abbreviation for the Ca2+/calmodulin-dependent protein kinase class of enzymes. CAMKs are ... Hudmon A, Schulman H (2002-06-01). "Neuronal CA2+/calmodulin-dependent protein kinase II: the role of structure and ...
... p39 interact with the alpha-subunit of Ca2+/calmodulin-dependent protein kinase II and alpha-actinin-1 in a calcium-dependent ... Sakashita G, Shima H, Komatsu M, Urano T, Kikuchi A, Kikuchi K (February 2003). "Regulation of type 1 protein phosphatase/ ... p39 interact with the alpha-subunit of Ca2+/calmodulin-dependent protein kinase II and alpha-actinin-1 in a calcium-dependent ... Agarwal-Mawal A, Paudel HK (June 2001). "Neuronal Cdc2-like protein kinase (Cdk5/p25) is associated with protein phosphatase 1 ...
... interleukin-1 receptor accessory protein-like 1 protein) PI4KIIIβ (type III phosphatidylinositol 4-kinase β) IP3 receptor (this ... PIK1 It binds to many proteins, some in calcium dependent and some in calcium independent ways, and switches many of the ... calcium-binding protein. Frq can substitute for calmodulin in some situations. It is thought to be associated with neuronal ... NCS-1 is a member of the neuronal calcium sensor family, a class of EF hand containing calcium-myristoyl-switch proteins. NCS-1 ...
Ca2+/calmodulin-dependent protein kinase II (CaMK II) is also activated by calcium influx through NMDA receptors, and is ... Lacosamide is used in combination with other types of medications to control various types of seizures, especially epilepsy. ... and cyclin-dependent protein kinase 5 (Cdk5) are highly expressed in Alzheimer's disease and are some of the protein kinases ... revealing the presence of both Rho kinase-dependent and Rho kinase-independent pathways for the growth cone collapse. In RhoA ...
... calcium/calmodulin-dependent protein kinase IV, and the peroxisome proliferator γ coactivator 1 (PGC-1). The Ras/MAPK signaling ... Calcineurin, a Ca2+/calmodulin-activated phosphatase implicated in nerve activity-dependent fiber-type specification in ... proteins and other regulatory proteins. Ca2+/calmodulin-dependent protein kinase activity is also upregulated by slow motor ... calcineurin and calcium/calmodulin-dependent protein kinase IV to activate transcription factors that regulate gene expression ...
... neural protein contains phosphorylation sites for cAMP-dependent protein kinase and calcium/calmodulin-dependent protein kinase ... Lack of synapsins altogether in neurons, leads to behavioral alterations as well as epileptic-type seizures. The lack affects ... and domain B has two mitogen-activated protein kinase phosphorylation sites. At its B domain, between amino acids 43 and 121, ... The synapsin II protein has been shown to interact with SYN1. Mutations in the SYN2 gene may be associated with abnormal ...
Zhang S, Ehlers MD, Bernhardt JP, Su CT, Huganir RL (August 1998). "Calmodulin mediates calcium-dependent inactivation of N- ... ". "Protein Information - Basic Information: Protein COPaKB ID: P35609". Cardiac Organellar Protein Atlas Knowledgebase. ... Young P, Ferguson C, Bañuelos S, Gautel M (March 1998). "Molecular structure of the sarcomeric Z-disk: two types of titin ... "Interactions of calmodulin and alpha-actinin with the NR1 subunit modulate Ca2+-dependent inactivation of NMDA receptors". The ...
... calcium/calmodulin-dependent serine protein kinase 3 and membrane-associated guanylate kinase 2. This gene is located on the ... Hsueh YP, Roberts AM, Volta M, Sheng M, Roberts RG (June 2001). "Bipartite interaction between neurofibromatosis type I protein ... "Entrez Gene: CASK Calcium/calmodulin-dependent serine protein kinase (MAGUK family)". Tarpey PS, Smith R, Pleasance E, Whibley ... Zhu ZQ, Wang D, Xiang D, Yuan YX, Wang Y (2014). "Calcium/calmodulin-dependent serine protein kinase is involved in exendin-4- ...
Calcium/calmodulin-dependent protein kinase type IV is an enzyme that in humans is encoded by the CAMK4 gene. The product of ... calmodulin-dependent protein kinase I by calmodulin and by Ca2+/calmodulin-dependent protein kinase kinase". J. Biol. Chem. 273 ... Phosphorylation by cyclic AMP-dependent protein kinase, protein kinase C, and calcium/calmodulin protein kinase; identification ... and activation of Ca2+-calmodulin-dependent protein kinase IV by Ca2+-calmodulin-dependent protein kinase Ia kinase. ...
... by protein kinase C and calcium/calmodulin kinase II in vitro". J. Neurochem. 71 (3): 1307-14. doi:10.1046/j.1471-4159.1998. ... Kubes M, Cordier J, Glowinski J, Girault JA, Chneiweiss H (1998). "Endothelin induces a calcium-dependent phosphorylation of ... "Molecular characterization of the human PEA15 gene on 1q21-q22 and association with type 2 diabetes mellitus in Pima Indians". ... "Protein kinase B/Akt binds and phosphorylates PED/PEA-15, stabilizing its antiapoptotic action". Mol. Cell. Biol. 23 (13): 4511 ...
The δ subunit is the ubiquitous eukaryotic protein calmodulin which itself has 4 calcium ion binding sites. When cytosolic Ca2+ ... but phosphorylation of both these subunits by protein kinase A (PKA, or cAMP-dependent protein kinase) reduces their respective ... Defects in phosphorylase kinase genes are the cause of glycogen storage disease type IX (GSD type IX) and GSD type VI (formerly ... In the active site, there is significant homology between PhK and other so-called P-loop protein kinases such as protein kinase ...
Cannabinoids reduce calcium influx by blocking the activity of voltage-dependent N-, P/Q- and L-type calcium channels. In ... through Gi/o-mediated inhibition of adenylyl cyclase and protein kinase A. Direct effects of CB1 receptors on membrane ... decreases in expression were seen in both calmodulin and ribosomal RNAs. In addition, CB1 activation has been demonstrated to ... Guo J, Ikeda SR (2004). "Endocannabinoids modulate N-type calcium channels and G-protein-coupled inwardly rectifying potassium ...
Calcium/calmodulin-dependent protein kinase type II gamma chain is an enzyme that in humans is encoded by the CAMK2G gene. The ... "Entrez Gene: CAMK2G calcium/calmodulin-dependent protein kinase (CaM kinase) II gamma". Moyers JS, Bilan PJ, Zhu J, Kahn CR ( ... Calcium/calmodulin-dependent protein kinase type II subunit gamma) at the PDBe-KB. Biology portal This article incorporates ... calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic ...
... and a calcium/calmodulin-dependent kinase (CAMK-1), and additional kinases, reaching its peak around mid-subjective day. Kinase ... with just one of the proteins present. The choice of which protein is made is the result of temperature-dependent splicing of ... Because sFRQ favors a longer period than lFRQ, free running rhythms in wild type Neurospora are somewhat decreased with ... A similar palette of kinases modifies the clock proteins in all cases. Negative feedback White Collar-1 Baker CL, Loros JJ, ...
... an effect which is associated with DAT phosphorylation through a Ca2+/calmodulin-dependent protein kinase (CAMK)-dependent ... calcium/calmodulin protein kinases) signaling, which, in addition to phosphorylating CREB, also phosphorylates HDAC5. Figure 2 ... ΔFosB is also induced in D1-type NAc MSNs by chronic consumption of several natural rewards, including sucrose, high-fat food, ... it triggers transporter phosphorylation via protein kinase A (PKA) and protein kinase C (PKC) signaling, ultimately resulting ...
Calcium/calmodulin-dependent protein kinase type II beta chain is an enzyme that in humans is encoded by the CAMK2B gene. The ... "Entrez Gene: CAMK2B calcium/calmodulin-dependent protein kinase (CaM kinase) II beta". Walikonis RS, Oguni A, Khorosheva EM, ... Sequence analyses of human brain calcium/calmodulin-dependent protein kinase II". Mol. Biol. Rep. 28 (1): 35-41. doi:10.1023/A: ... Novak G, Seeman P, Tallerico T (2001). "Schizophrenia: elevated mRNA for calcium-calmodulin-dependent protein kinase IIbeta in ...
This calcium then interacts with calcineurin and calcium/calmodulin-dependent kinases that in turn activate transcription ... October 2004). "Endoplasmic reticulum stress links obesity, insulin action, and type 2 diabetes". Science. 306 (5695): 457-61. ... "Coupling of stress in the ER to activation of JNK protein kinases by transmembrane protein kinase IRE1". Science. 287 (5453): ... PERK (protein kinase RNA-like endoplasmic reticulum kinase) activates itself by oligomerization and autophosphorylation of the ...
Barry FA, Gibbins JM (Apr 2002). "Protein kinase B is regulated in platelets by the collagen receptor glycoprotein VI". The ... Andrews RK, Suzuki-Inoue K, Shen Y, Tulasne D, Watson SP, Berndt MC (Jun 2002). "Interaction of calmodulin with the cytoplasmic ... Collagen fibers are the most thrombogenic macromolecular components of the extracellular matrix, with collagen types I, III, ... "Expression and function of the mouse collagen receptor glycoprotein VI is strictly dependent on its association with the ...
... the microtubule-associated tau proteins and S100b regulate tau phosphorylation by the Ca2+/calmodulin-dependent protein kinase ... "The calcium-modulated proteins, S100A1 and S100B, as potential regulators of the dynamics of type III intermediate filaments". ... S100 calcium-binding protein B (S100B) is a protein of the S-100 protein family. S100 proteins are localized in the cytoplasm ... the microtubule-associated tau proteins and S100b regulate tau phosphorylation by the Ca2+/calmodulin-dependent protein kinase ...
"Calmodulin is involved in the Ca2+-dependent activation of ceramide kinase as a calcium sensor". J. Biol. Chem. 280 (49): 40436 ... CERK shares sequence homology with sphingosine kinase type I, including an N-terminal pleckstrin homology (PH) domain and a ... It was demonstrated that C-1-P perpetuates the phosphorylation of glycogen synthase kinase-3 β and retinoblastoma protein, ... CERK was later confirmed to bind calmodulin in the presence of calcium, indicating the calmodulin first binds calcium and then ...
ITP3K activity is indirectly stimulated by phosphorylation by calcium/calmodulin-dependent kinase II (CaMKII). In addition, ... and these studies revealed various feedback mechanisms by which the enzyme is regulated by calcium and protein kinases. In 1999 ... the gene becomes expressed in a number of cancer cell types. In most cases, ITP3K expression causes the cancer to be more ... ITP3Ks are stimulated directly by calcium/calmodulin (Ca2+/CaM) binding. Generally, mammalian ITP3Ks are activated by calcium ...
"Structure and expression of a calcium-binding protein gene contained within a calmodulin-regulated protein kinase gene". ... encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. Four transcript variants that produce four ... Reaction type: phospho group transfer) It requires Ca2+ and calmodulin for activity. The 20-kDa light chain from smooth muscle ... "Kinase-related protein (telokin) is phosphorylated by smooth-muscle myosin light-chain kinase and modulates the kinase activity ...
... s are also modulated by G protein-coupled receptors and the adrenergic nervous system. Protein Kinase A ( ... "Switching off calcium-dependent inactivation in L-type calcium channels by an autoinhibitory domain". Proceedings of the ... As the pore opens and causes an influx of Calcium, calcium binds to calmodulin and then interacts with the loop that connects ... P-type, and N-type. L-type calcium channels were peptide sequenced and it was found that there were 4 kinds of L-type calcium ...
2005). "Multivalent interactions of calcium/calmodulin-dependent protein kinase II with the postsynaptic density proteins NR2B ... 2007). "Densin and filtrin in the pancreas and in the kidney, targets for humoral autoimmunity in patients with type 1 diabetes ... 2001). "Densin-180 forms a ternary complex with the (alpha)-subunit of Ca2+/calmodulin-dependent protein kinase II and (alpha)- ... portray Densin-180 as a key interactor in the midst of receptor proteins, scaffolding proteins and structural proteins. [number ...
Ca2+/calmodulin-dependent protein kinase II (CaMKII) plays a crucial role in a type of synaptic plasticity known as long-term ... Many of the proteins that calmodulin binds are unable to bind calcium themselves, and use calmodulin as a calcium sensor and ... Proteopedia page for Calmodulin and its conformational change Protein kinase Ca2+/calmodulin-dependent protein kinase Stevens ... This MLC kinase is activated by a calmodulin when it is bound by calcium, thus making smooth muscle contraction dependent on ...
Like neuroligins, neurexins possess a PDZ-domain that associates with CASK (Calcium-calmodulin-dependent protein kinase). In ... Multiple nectins on one cell membrane will bind together at the V-type loop to form a cluster of nectin proteins, a process ... Cadherins are calcium- dependent, homophilic cell adhesion molecules that form complexes with cytosolic partners known as ... This interaction, mediated by ephrin A3/EphA4 signaling, induces the recruitment and activation of cyclin-dependent kinase 5 ( ...
... independent pathway involving calcium-binding protein 39 (Cab39) and serine threonine kinase with no lysine member 4 (WNK4) in ... WNK4 enhances TRPV5 by increasing its forward trafficking to the plasma membrane in a kinase-dependent manner. The inhibitory ... However, when Ca2+ levels are elevated, Ca2+/calmodulin complex binds to the C-terminal region, derepressing WNK4 kinase ... August 2005). "A new kindred with pseudohypoaldosteronism type II and a novel mutation (564D>H) in the acidic motif of the WNK4 ...
... p39 interact with the alpha-subunit of Ca2+/calmodulin-dependent protein kinase II and alpha-actinin-1 in a calcium-dependent ... Actinin, alpha 1 has been shown to interact with: CDK5R1, CDK5R2, Collagen, type XVII, alpha 1, GIPC1, PDLIM1, Protein kinase ... Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal ... Bunn RC, Jensen MA, Reed BC (1999). "Protein interactions with the glucose transporter binding protein GLUT1CBP that provide a ...
Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C is an enzyme that in humans is encoded by the PDE1C ... Tat protein down-regulates CREB transcription factor expression in PC12 neuronal cells through a phosphatidylinositol 3-kinase/ ... 2006). "Subcellular localization and regulation of type-1C and type-5 phosphodiesterases". Biochem. Biophys. Res. Commun. 341 ( ... "Entrez Gene: PDE1C phosphodiesterase 1C, calmodulin-dependent 70kDa". Vandeput F, Wolda SL, Krall J, et al. (2007). "Cyclic ...
Calcium channel, voltage-dependent, L type, alpha 1C subunit (also known as Cav1.2) is a protein that in humans is encoded by ... When depolarization occurs, calcium ions flow through the channel and some bind to Calmodulin. The Calcium/Calmodulin binding ... Perets T, Blumenstein Y, Shistik E, Lotan I, Dascal N (Apr 1996). "A potential site of functional modulation by protein kinase ... This gene encodes an alpha-1 subunit of a voltage-dependent calcium channel. Calcium channels mediate the influx of calcium ...
positive regulation of non-membrane spanning protein tyrosine kinase activity. • transmembrane receptor protein tyrosine kinase ... TrkB autophosphorylation is dependent upon its ligand-specific association with BDNF, a widely expressed activity-dependent ... triggering a calcium influx. Through a protein signaling cascade requiring Erk, CaM KII/IV, PI3K, and PLC, NMDA receptor ... Blockading BDNF signaling with a tyrosine kinase inhibitor or a PKC inhibitor in wild type mice produced significant reductions ...
S6 kinase, protein kinase A (PKA), protein kinase C, Cdc2, and/or a Ca2+/calmodulin-dependent protein kinase; b) moves to bind ... Aberrant expression of LOX5 is seen in various types of human cancer tumors in vivo as well as in various types of human cancer ... Hammarberg T, Reddy KV, Persson B, Rådmark O (2002). "Calcium binding to 5-lipoxygenase". Adv. Exp. Med. Biol. Advances in ... ALOX5 binds with the F actin-binding protein, coactin-like protein. Based on in vitro studies, this protein binding serves to ...
Calcium/calmodulin-dependent protein kinases. References[edit]. .mw-parser-output .reflist{font-size:90%;margin-bottom:0.5em; ... These types of experiments have shown that different pathways are being activated or regulated depending on the location of the ... which contain residues that can be directly modified by a series of protein kinases and protein phosphatases, as well as ... "MHC class I immune proteins are critical for hippocampus-dependent memory and gate NMDAR-dependent hippocampal long-term ...
"Calcium/calmodulin-dependent protein kinase II phosphorylation drives synapse-associated protein 97 into spines". J. Biol. Chem ... "Ca2+/calmodulin-kinase II enhances channel conductance of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate type glutamate ... is the influx of calcium through the NMDA receptors and the resultant activation of Ca2+/calmodulin-dependent protein kinase ( ... "Long-term potentiation is associated with an increased activity of Ca2+/calmodulin-dependent protein kinase II". J. Biol. Chem ...
The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein composed of five extracellular cadherin repeats, a ... "The tyrosine kinase substrate p120cas binds directly to E-cadherin but not to the adenomatous polyposis coli protein or alpha- ... "Entrez Gene: CDH1 cadherin 1, type 1, E-cadherin (epithelial)".. *^ Fleming TP, Papenbrock T, Fesenko I, Hausen P, Sheth B ( ... Li Z, Kim SH, Higgins JM, Brenner MB, Sacks DB (December 1999). "IQGAP1 and calmodulin modulate E-cadherin function". The ...
... which leads to hyperpolarization and relaxation Stimulates a cGMP-dependent protein kinase that activates myosin light chain ... Nitric oxide is mediated in mammals by the calcium-calmodulin controlled isoenzymes eNOS (endothelial NOS) and nNOS (neuronal ... It is synthesized by many cell types in response to cytokines and is an important factor in the response of the body to attack ... NOSs can be dimeric, calmodulin-dependent or calmodulin-containing cytochrome p450-like hemoprotein that combines reductase and ...
see MAP kinase pathway. Calcium. *Intracellular calcium-sensing proteins. *Calcineurin. *Ca2+/calmodulin-dependent protein ... type 1 angiotensin receptor binding. • protein complex binding. • signal transducer activity. • protein binding. • GTPase ... protein heterotrimerization. • Wnt signaling pathway, calcium modulating pathway. • protein folding. • G-protein coupled ... phospholipase C-activating G-protein coupled receptor signaling pathway. • retina development in camera-type eye. • Ras protein ...
The increase in presynaptic calcium concentration activates calcium-calmodulin-dependent protein kinase (CaMK). CaMK ... The protein dense projections vary in size and shape depending on the type of synapse examined. One striking example of the ... Voltage-dependent calcium channel (VDCC) Allows the rapid influx of calcium during an action potential. ... The protein ELKS binds to the cell adhesion protein, β-neurexin, and other proteins within the complex such as Piccolo and ...
... is phosphorylated by the calcium-calmodulin-dependent protein kinase.[27] MAPKAPK2 (mitogen-activated-protein kinase-activating ... embryonic camera-type eye morphogenesis. • social organism behavior. • cellular response to manganese ion. • response to ether ... that are phosphorylated by a variety of protein kinases.[12][25] Ser40 is phosphorylated by the cAMP-dependent protein kinase.[ ... "Molecular cloning of cDNA coding for brain-specific 14-3-3 protein, a protein kinase-dependent activator of tyrosine and ...
see MAP kinase pathway. Calcium. *Intracellular calcium-sensing proteins. *Calcineurin. *Ca2+/calmodulin-dependent protein ... Types of G protein signaling[edit]. G protein can refer to two distinct families of proteins. Heterotrimeric G proteins, ... Heterotrimeric G proteins[edit]. Main article: Heterotrimeric G proteins. Different types of heterotrimeric G proteins share a ... 3 Types of G protein signaling *3.1 Heterotrimeric G proteins *3.1.1 Common mechanism *3.1.1.1 Activation ...
... but most prominently by Rho-dependent kinase and/or by the calcium-calmodulin-dependent myosin light chain kinase, not only ... "Cleavage of human and mouse cytoskeletal and sarcomeric proteins by human immunodeficiency virus type 1 protease. Actin, desmin ... protein binding. • ADP binding. • protein membrane anchor. • actin-dependent ATPase activity. • calmodulin binding. • ATPase ... Other proteins that are known to interact with NM IIA include the actin binding protein tropomyosin 4.2 [26] and a novel actin ...
It was regulated by Ca2+ via a calmodulin kinase II-dependent mechanism. Distant homologues may be present in plants, ciliates ... demonstrated that CLIC proteins have glutaredoxin-like glutathione-dependent oxidoreductase enzymatic activity. CLICs 1, 2 and ... Agnel, M.; Vermat, T.; Culouscou, J. M. (1999-07-23). "Identification of three novel members of the calcium-dependent chloride ... In terms of primary structure, they are unrelated to known cation channels or other types of anion channels. Three CLC ...
Calcium binds to proteins such as calmodulin (CaM) and an eye-specific protein kinase C (PKC) known as InaC. These proteins ... y-type) and other mutants that show a mosaic pigment pattern with some regions of the cuticle (wild type, y2-type).[69] The ... Sepia colored eyes are not dependent on the sex of the fly. The Sepia eye color decreases sexual activity in males and ... In addition, proteins called arrestins bind metarhodopsin and prevent it from activating more Gq. A sodium-calcium exchanger ...
... in which calcium-modulated protein calmodulin (CaM) binds Ca2+, undergoes a change in conformation, and activates CaM kinase II ... Phosphorylation by cAMP-dependent protein kinasesEdit. Cyclic AMP-dependent protein kinases (protein kinase A) are activated by ... Further effect depends on the type of G protein. G proteins are subsequently inactivated by GTPase activating proteins, known ... The G protein-coupled receptor kinases (GRKs) are protein kinases that phosphorylate only active GPCRs.[53] G-protein-coupled ...
see MAP kinase pathway. Calcium. *Intracellular calcium-sensing proteins. *Calcineurin. *Ca2+/calmodulin-dependent protein ... Types[edit]. There are membrane-bound (type 1, guanylate cyclase-coupled receptor) and soluble (type 2, soluble guanylate ... Depending on cell type, it can drive adaptive/developmental changes requiring protein synthesis. In smooth muscle, cGMP is the ... Low concentrations of calcium cause the dimerization of RETGC-1 proteins through stimulation from guanylate cyclase-activating ...
... protein kinase Cs, calmodulin-modulated myosin light chain kinase, RAF/MEK/Mitogen-activated protein kinases, PKC/Ca2+/ ... IP stimulates one or more of three types of G protein complexes, depending on cell type: a) Gs alpha subunit-Gβγ complexes ... G-protein coupled receptor signaling pathway. • inflammatory response. • positive regulation of cytosolic calcium ion ... stimulates adenyl cyclase to raise intracellular levels of cAMP and thereby activate cAMP-regulated protein kinases A-dependent ...
"The tyrosine kinase substrate p120cas binds directly to E-cadherin but not to the adenomatous polyposis coli protein or alpha- ... "Entrez Gene: CDH1 cadherin 1, type 1, E-cadherin (epithelial)".. *↑ Fleming TP, Papenbrock T, Fesenko I, Hausen P, Sheth B ( ... Li Z, Kim SH, Higgins JM, Brenner MB, Sacks DB (December 1999). "IQGAP1 and calmodulin modulate E-cadherin function". J. Biol. ... Jiang MC, Liao CF, Tai CC (June 2002). "CAS/CSE 1 stimulates E-cadhrin-dependent cell polarity in HT-29 human colon epithelial ...
... activation of Ca2+/calmodulin-dependent protein kinase II can be cAMP dependent or independent.[15] ... Type of reinforcer Sources Opiates Psychostimulants High fat or sugar food Sexual intercourse Physical exercise. (aerobic) ... increasing intracellular calcium among other cAMP mediated processes. The D2 class of receptors produce the opposite effect, as ... cAMP mediated protein kinase A activity also results in the phosphorylation of DARPP-32, an inhibitor of protein phosphatase 1 ...
see MAP kinase pathway. Calcium. *Intracellular calcium-sensing proteins. *Calcineurin. *Ca2+/calmodulin-dependent protein ... which vary based on the type of cell. ... cAMP-dependent protein kinase), one of the first few kinases ... Main article: function of cAMP-dependent protein kinase. In humans, cAMP works by activating protein kinase A (PKA, ... an enzyme called protein kinase A (PKA).[12]. The PKA enzyme is also known as cAMP-dependent enzyme because it gets activated ...
A calcium-calmodulin complex also acts to inhibit the binding of cAMP to the cAMP-dependent channel, thus contributing to ... in that cAMP works by directly binding to the ion channel rather than through activation of protein kinase A. It is similar to ... Because several receptor types are activated due to the different chemical features of the odorant, several glomeruli are ... In mammals, the odorants stimulate adenylate cyclase to synthesize cAMP via a G protein called Golf. cAMP, which is the second ...
see MAP kinase pathway. Calcium. *Intracellular calcium-sensing proteins. *Calcineurin. *Ca2+/calmodulin-dependent protein ... Types[edit]. There are several different cyclins that are active in different parts of the cell cycle and that cause the Cdk to ... Cyclins, when bound with the dependent kinases, such as the p34/cdc2/cdk1 protein, form the maturation-promoting factor. MPFs ... is a family of proteins that control the progression of cells through the cell cycle by activating cyclin-dependent kinase (CDK ...
Opening of the L-type calcium channel causes influx of extracellular Ca2+, which then binds calmodulin. The activated ... The intracellular β subunit (55 kDa) is an intracellular MAGUK-like protein (Membrane-Associated Guanylate Kinase) containing a ... Voltage-gated calcium channels (VGCCs), also known as voltage-dependent calcium channels (VDCCs), are a group of voltage-gated ... opening of the L-type calcium channel permits influx of calcium into the cell. The calcium binds to the calcium release ...
... calcium/calmodulin-dependent protein kinase IV, and the peroxisome proliferator γ coactivator 1 (PGC-1). The Ras/MAPK signaling ... Calcineurin, a Ca2+/calmodulin-activated phosphatase implicated in nerve activity-dependent fiber-type specification in ... proteins and other regulatory proteins. Ca2+/calmodulin-dependent protein kinase activity is also upregulated by slow motor ... calcineurin and calcium/calmodulin-dependent protein kinase IV to activate transcription factors that regulate gene expression ...
It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is ... Holmes AM (1996). "In vitro phosphorylation of human immunodeficiency virus type 1 Tat protein by protein kinase C: evidence ... kinase activity. • protein binding. • ATP binding. • protein serine/threonine kinase activity. • protein kinase activity. ... Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by the second ...
... suggesting that inhibition of Ca2+/calmodulin-dependent protein kinase II may also be a potential treatment strategy.[15][16] ... which can be explained by decreased voltage-dependent calcium channel expression, specifically Ca(v)1.3, which is responsible ... Initially, a Glu1425Gly mutation in ANK2 was found to cause dominantly-inherited long QT syndrome, type 4, cardiac arrhythmia. ... Effects on ryanodine receptors specifically were also rescued by a potent Ca2+/calmodulin-dependent protein kinase II inhibitor ...
Opening of the L-type calcium channel causes influx of extracellular Ca2+, which then binds calmodulin. The activated ... The intracellular β subunit (55 kDa) is an intracellular MAGUK-like protein (Membrane-Associated Guanylate Kinase) containing a ... Voltage-gated calcium channels (VGCCs), also known as voltage-dependent calcium channels (VDCCs), are a group of voltage-gated ... P-type calcium channel ("Purkinje") /Q-type calcium channel. HVA (high voltage activated). Cav2.1 (CACNA1A). α2δ, β, possibly γ ...
... through a protein kinase C-dependent mechanism". Biochem J. 466 (2): 379-390. doi:10.1042/bj20140881. PMID 25422863.. ... The effects of calcium on human cells are specific, meaning that different types of cells respond in different ways. However, ... and calmodulin, proteins that are necessary for promoting contraction in muscle. ... Activation of protein kinase C. Further reading: Function of protein kinase C. ...
Protein KinasesProtein-Serine-Threonine KinasesCalcium-Calmodulin-Dependent Protein KinasesCalcium-Calmodulin-Dependent Protein ... Signaling Peptides and ProteinsCalcium-Calmodulin-Dependent Protein KinasesCalcium-Calmodulin-Dependent Protein Kinase Type 2 ... Signaling Peptides and ProteinsCalcium-Calmodulin-Dependent Protein KinasesCalcium-Calmodulin-Dependent Protein Kinase Type 2 ... Calmodulin-Dependent Protein Kinase II. *Calmodulin Dependent Protein Kinase II. *Calcium-Calmodulin-Dependent Protein Kinase ...
calcium/calmodulin-dependent protein kinase type II subunit alpha isoform 2 [Homo sapiens]. NCBI Reference Sequence: NP_ ... calcium/calmodulin-dependent protein kinase type II subunit alpha isoform 2 [Hom... calcium/calmodulin-dependent protein kinase ... The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein ... Transcript/Protein Information [PANTHER Classification System] Transcript/Protein Information. PANTHER Classification System ...
... calcium/calmodulin-dependent protein kinase II) in amphetamine-produced conditioned place preference (AMPH-CPP) in rats. An ... Calcium-Calmodulin-Dependent Protein Kinase Type 2 * Calcium-Calmodulin-Dependent Protein Kinases / drug effects* ... Roles of hippocampal N-methyl-D-aspartate receptors and calcium/calmodulin-dependent protein kinase II in amphetamine-produced ... calcium/calmodulin-dependent protein kinase II) in amphetamine-produced conditioned place preference (AMPH-CPP) in rats. An ...
... forms a highly conserved dodecameric assembly that is sensitive to the frequency of calcium pulse trains. Neither the structure ... Calcium-Calmodulin-Dependent Protein Kinase Type 2 / chemistry* * Calcium-Calmodulin-Dependent Protein Kinase Type 2 / ... Calcium/calmodulin-dependent kinase II (CaMKII) forms a highly conserved dodecameric assembly that is sensitive to the ... A mechanism for tunable autoinhibition in the structure of a human Ca2+/calmodulin- dependent kinase II holoenzyme Cell. 2011 ...
This indicates the type of evidence that supports the existence of the protein. Note that the protein existence evidence does ... View protein in PROSITE. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 ... View protein in PROSITE. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 ... Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha, isoform CRA_aImported. ,p>Information which has been ...
... Daniels, Lorna Jane ... Cite this item: Daniels, L. J. (2017). The role of calcium calmodulin dependent protein kinase II in type 2 diabetic heart ... has shown that diabetic patients and mouse models of diabetes have an up-regulation of calmodulin-dependent protein kinase ... Type 2 diabetes is a well-established risk factor for cardiovascular disease, with the landmark Framingham study in the 1970s ...
L-type calcium channel; MCU, mitochondria calcium uniporter; NCX, Na+/Ca2+ exchanger; NHE, Na+/H+ exchanger; PKA, protein ... Calcium-calmodulin dependent protein kinase II (CaMKII): a main signal responsible for early reperfusion arrhythmias. J Mol ... In Vivo Post-Cardiac Arrest Myocardial Dysfunction Is Supported by Ca2+/Calmodulin-Dependent Protein Kinase II-Mediated Calcium ... E, Proposed mechanism for PMAD, mediated by calcium overload, Ca2+/calmodulin-dependent protein kinase II (CaMKII), and ...
... and CaMKII alpha and beta protein levels. In the hippocampus of Pb2+-exposed 50-day-old rats known to exhib ... we examined whether calcium/calmodulin-dependent protein kinase II (CaMKII) is affected by chronic developmental Pb2+ exposure ... Calcium/calmodulin dependent protein kinase II (CaMKII); NMDA receptor (NMDAR); Lead (Pb2+); Calcium signaling; Enzyme kinetics ... Calcium/calmodulin-dependent protein kinase II activity and expression are altered in the hippocampus of Pb2+- exposed rats.. ...
... and BDNF-dependent mechanisms and their crosstalk in the hippocampus, including potential implications for the pathogenesis of ... dependent mechanisms and their crosstalk in the hippocampus, including potential implications for the pathogenesis of common ... tropomyosin-related kinase receptor B (TrkB). This review addresses the effects of ELS paradigms on GC- and BDNF- ... tropomyosin-related kinase receptor B (TrkB). This review addresses the effects of ELS paradigms on GC- ...
Calcium/calmodulin-dependent protein kinase kinase 2: roles in signaling and pathophysiology. J Biol Chem. 2012;287(38):31658- ... Macroscopic raw traces of L-type calcium current comparing IV-3 against CRISPR-corrected IV-3. (H) Summary L-type calcium ... Regulation of voltage-dependent calcium channels by RGK proteins. Biochim Biophys Acta. 2013;1828(7):1644-1654.. View this ... L-type calcium current density increase revealed by patient-specific iPSC-CMs. (A) Representative macroscopic whole-cell L-type ...
... the mechanisms that underlie experience-dependent transcriptional regulation of the rate-limiting serotonin biosynthetic enzyme ... Caenorhabditis elegans Proteins / genetics*, metabolism. Calcium-Calmodulin-Dependent Protein Kinase Type 2 / genetics*, ... EC 2.7.11.17/Calcium-Calmodulin-Dependent Protein Kinase Type 2; EC 2.7.11.17/unc-43 protein, C elegans; EC 3.6.5.1/GTP-Binding ... Upstream of ADF, the Gqα protein EGL-30 facilitates training-dependent induction of tph-1 by functional regulation of olfactory ...
CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE TYPE I (EC 2.7.1.123). GN CMK1. 3. entry: SWISSPROT:KCC2_YEAST DE CALCIUM/ ... CALMODULIN-DEPENDENT PROTEIN KINASE TYPE II (EC 2.7.1.123). GN CMK2. 4. entry: SWISSPROT:P2B1_YEAST DE PROTEIN PHOSPHATASE 2B ... calmodulin is an essential protein. MH - Amino Acid Sequence MH - Base Sequence MH - Calcium/METABOLISM MH - Calmodulin/* ... CALMODULIN-BINDING PROTEIN 1). GN CNA1 OR CMP1. 5. entry: SWISSPROT:P2B2_YEAST DE PROTEIN PHOSPHATASE 2B CATALYTIC SUBUNIT A2 ( ...
Calcium-Calmodulin-Dependent Protein Kinase Type 2 Calcium-Calmodulin-Dependent Protein Kinases ... keywords = "Ca/CaM-dependent protein kinase II, Calcium, Calmodulin, Na-channel",. author = "Takeshi Aiba and Hesketh, { ... Na+ channel regulation by Ca2+/calmodulin and Ca 2+/calmodulin-dependent protein kinase II in guinea-pig ventricular myocytes. ... Na+ channel regulation by Ca2+/calmodulin and Ca 2+/calmodulin-dependent protein kinase II in guinea-pig ventricular myocytes. ...
Calmodulin-dependent protein kinase II (CaMKII) signaling in the heart regulates cardiomyocyte contractility and growth in ... Therefore, CaMKIIδB may serve as a novel modulatory protein to enhance CPC survival and commitment into the cardiac and smooth ... CPCs undergoing early differentiation in vitro increase levels of CaMKIIδB in the nuclear compartment where the kinase may ... Dexamethasone (Dex) treatment increased mRNA and protein expression of cardiomyogenic markers cardiac troponin T and α-smooth ...
The role of calcium calmodulin dependent protein kinase II in type 2 diabetic cardiac dysfunction. Verbal presentation at the ... The role of calcium calmodulin dependent protein kinase II in type 2 diabetic cardiac dysfunction. Verbal presentation at the ... of calcium/calmodulin-dependent kinase II restores contraction and relaxation in isolated cardiac muscle from type 2 diabetic ... of calcium/calmodulin-dependent kinase II restores contraction and relaxation in isolated cardiac muscle from type 2 diabetic ...
The role of calcium calmodulin dependent protein kinase II in type 2 diabetic cardiac dysfunction. Verbal presentation at the ... effect of calcium/calmodulin-dependent kinase II on Cav1.2 Protein. Heart, Lung & Circulation, 26(Suppl. 2), (pp. S109). doi: ... of calcium/calmodulin-dependent protein kinase II δ. Journal of Biological Chemistry, 290(42), 25646-25656. doi: 10.1074/jbc. ... of calcium/calmodulin-dependent kinase II restores contraction and relaxation in isolated cardiac muscle from type 2 diabetic ...
Calcium/calmodulin-dependent protein kinase types II and IV differentially regulate CREB-dependent gene expression. Mol Cell ... Park IK, Soderling TR: Activation of Ca2+/calmodulin-dependent protein kinase (CaM-kinase) IV by CaM-kinase kinase in Jurkat T ... Enslen H, Soderling TR: Roles of calmodulin-dependent protein kinases and phosphatase in calcium-dependent transcription of ... molecular cloning and expression of calcium/calmodulin-dependent protein kinase kinase. J Biol Chem270 :19320 -19324,1995. ...
CalciumIBA 06/2015. 1. Calcium-Calmodulin-Dependent Protein Kinase Type 2IBA 06/2015. ... Involvement of hypothalamic AMP-activated protein kinase in leptin-induced sympathetic nerve activation.. ... the hypothalamus regulates hepatic autonomic nerve activity via phosphatidylinositol 3-kinase and AMP-activated protein kinase. ... Neuronal receptor activity-modifying protein 1 promotes energy expenditure in mice.. For more, sign up at right for free... ...
L-type calcium channel; CREB, cAMP response element-binding protein. ... When calcium/calmodulin-dependent calmodulin protein kinase II activity in the lumbar spinal cord after acute i.t. ... the increase in calcium/calmodulin-dependent calmodulin protein kinase II activity was not significant reduced in α7 KO mice ... Nicotinic Regulation of Calcium/Calmodulin-Dependent Protein Kinase II Activation in the Spinal Cord. M. I. Damaj ...
"CAMK2A calcium/calmodulin dependent protein kinase II alpha [Homo sapiens (human)] - Gene - NCBI". www.ncbi.nlm.nih.gov. ... β CAMKIIδ CAMKIIγ CAMKIII CAMKIV CAMKV CaM kinase like vesicle associated SCAMK Ca2+/calmodulin-dependent protein kinase kinase ... CAMK, also written as CaMK, is an abbreviation for the Ca2+/calmodulin-dependent protein kinase class of enzymes. CAMKs are ... Hudmon A, Schulman H (2002-06-01). "Neuronal CA2+/calmodulin-dependent protein kinase II: the role of structure and ...
Myc-DDK-tagged ORF clone of Homo sapiens calcium/calmodulin-dependent protein kinase II delta (CAMK2D), transcript variant 2 as ... Product types:. - all types -. Antibodies. Assays & Kits. Blood products. cDNA clones. Cell lines. Cells & Human Biospecimens. ... home , products , origene , myc-ddk-tagged orf clone of homo sapiens calcium/calmodulin-dependent protein kinase ii delta ( ... Myc-DDK-tagged ORF clone of Homo sapiens calcium/calmodulin-dependent protein kinase II delta (CAMK2D), transcript variant 2 as ...
Calcium/metabolism*. *Calcium-Calmodulin-Dependent Protein Kinase Type 2/metabolism*. *Calmodulin/metabolism* ... Dendrite formation involves activation of Ca2+/Calmodulin (CaM)-dependent kinase II (CaMKII) by CaM. Previous work showed that ... Dendrite formation involves activation of Ca2+/Calmodulin (CaM)-dependent kinase II (CaMKII) by CaM. Previous work showed that ... Mentions: Stimulation of MEL receptors (MT1/2) causes PKC activation that has been shown to be involved in physiological ...
CAMK4 is calcium/calmodulin-dependent protein kinase, that via phosphorylation, triggers the CaMKK-CaMK4 signaling cascade and ... MEF2 responds to multiple calcium-regulated signals in the control of skeletal muscle fiber type. Embo j 19, 1963-1973, https ... Profiling of human lymphocytes reveals a specific network of protein kinases modulated by endurance training status *Katharina ... Corepressor-dependent silencing of chromosomal regions encoding neuronal genes. Science (New York, N.Y.) 298, 1747-1752, https ...
Hunting Increases Phosphorylation of Calcium/Calmodulin-Dependent Protein Kinase Type II in Adult Barn Owls. Grant S. Nichols ... "Axodendritic contacts onto calcium/calmodulin-dependent protein kinase type II-expressing neurons in the barn owl auditory ... A. Hudmon and H. Schulman, "Neuronal CA2+/calmodulin-dependent protein kinase II: the role of structure and autoregulation in ... "Deficient hippocampal long-term potentiation in alpha-calcium-calmodulin kinase II mutant mice," Science, vol. 257, no. 5067, ...
Calcium-Calmodulin-Dependent Protein Kinase Type 2/antagonists & inhibitors/metabolism. *Disease Models, Animal ... Calcium-Calmodulin-Dependent Protein Kinase Type 2/antagonists & inhibitors/metabolism. *Disease Models, Animal ...
... temporal and region-specific requirements of the beta isoform of Calcium/Calmodulin-dependent protein kinase type 2 (CAMK2B) in ... temporal and region-specific requirements of the beta isoform of Calcium/Calmodulin-dependent protein kinase type 2 (CAMK2B) in ... Kinase activity is not required for αCaMKII-dependent presynaptic plasticity at CA3-CA1 synapses *Mohammad Reza Hojjati ... Rights & permissionsfor article Kinase activity is not required for αCaMKII-dependent presynaptic plasticity at CA3-CA1 ...
... calcium/calmodulin-dependent protein kinase type II subunit alpha 101168431 calcium/calmodulin-dependent protein kinase type II ... calcium/calmodulin-dependent protein kinase (CaM kinase) II [EC:2.7.11.17] K04515 CAMK2; calcium/calmodulin-dependent protein ... calcium/calmodulin-dependent protein kinase (CaM kinase) II [EC:2.7.11.17] K04515 CAMK2; calcium/calmodulin-dependent protein ... calcium/calmodulin-dependent protein kinase (CaM kinase) II [EC:2.7.11.17] K04515 CAMK2; calcium/calmodulin-dependent protein ...
... sensing serine/threonine-kinases. Ca(2+)/calmodulin-dependent protein kinase II is highly expressed in insulin secreting cells ... calmodulin-dependent protein kinase II gamma and of beta-cell specific proteins such as the homeodomain factor pancreatic- ... This caused a loss of Ca(2+)/calmodulin-dependent protein kinase II delta(2) expression at the mRNA and protein level, while ... To elucidate its function the antisense sequence of the major beta-cell subtype, Ca(2+)/calmodulin-dependent protein kinase II ...
Calcium-Calmodulin-Dependent Protein Kinase Type 2 Calcium-Calmodulin-Dependent Protein Kinases ... Mutational analysis of Ca2+-independent autophosphorylation of calcium/calmodulin-dependent protein kinase II. In: Journal of ... Mutational analysis of Ca2+-independent autophosphorylation of calcium/calmodulin-dependent protein kinase II. / Mukherji, ... title = "Mutational analysis of Ca2+-independent autophosphorylation of calcium/calmodulin-dependent protein kinase II", ...
Calcium Amino Acids Bearings (structural) Calcium-Calmodulin-Dependent Protein Kinase Type 2 ... T2 - Conformational changes induced by calmodulin-binding peptides from myosin light chain kinase and protein kinase II ... or calmodulin-dependent protein kinase II (CaMPK-II), respectively. Both steady-state and time-resolved fluorescence data were ... or calmodulin-dependent protein kinase II (CaMPK-II), respectively. Both steady-state and time-resolved fluorescence data were ...
  • Calcium/calmodulin-dependent kinase II (CaMKII) forms a highly conserved dodecameric assembly that is sensitive to the frequency of calcium pulse trains. (nih.gov)
  • We present the crystal structure of an autoinhibited full-length human CaMKII holoenzyme, revealing an unexpected compact arrangement of kinase domains docked against a central hub, with the calmodulin-binding sites completely inaccessible. (nih.gov)
  • Comparison of CaMKII isoforms, which differ in the length of the linker between the kinase domain and the hub, demonstrates that these interactions can be strengthened or weakened by changes in linker length. (nih.gov)
  • Recent evidence has shown that diabetic patients and mouse models of diabetes have an up-regulation of calmodulin-dependent protein kinase delta (CaMKIIδ) activity. (otago.ac.nz)
  • CaMKIIδ is a multifunctional serine-threonine kinase expressed as four different genes that generate four isoforms (αβγδ) and a number of splice variants. (otago.ac.nz)
  • Upon CaMKIIδ activation is involved in coordination of the ion channels and Ca2+ handling proteins involved in excitation-contraction and excitation-transcription coupling in the myocardium. (otago.ac.nz)
  • Therefore, this thesis set out to investigate the role of CaMKIIδ in the type 2 diabetic heart and the potential therapeutic role for CaMKIIδ inhibitors. (otago.ac.nz)
  • To investigate whether CaMKIIδ activation is increased prior to systolic dysfunction in the type 2 diabetic heart western blots were carried out to measure the activation levels of CaMKIIδ in leptin-receptor-deficient 20-week-old male non-diabetic and type 2 diabetic Zucker diabetic fatty (ZDF) rats. (otago.ac.nz)
  • Western blots confirmed that there was no change in expression of CaMKIIδ (0.95 ± 0.08 vs 1.15 ± 0.10, nDM (n=8) vs DM (n=8) but CaMKIIδ phosphorylation was significantly increased in the DM animals (0.77 ± 0.13 vs 1.40 ± 0.26) indicating that CaMKIIδ activation was increased in the type 2 diabetic ZDF heart prior to systolic dysfunction. (otago.ac.nz)
  • To investigate the mechanisms behind the reduced contractility in the type 2 diabetic trabeculae and the effects of CaMKIIδ inhibition, cardiomyocytes were isolated from 18-19 week old male nDM (n=6) and DM (n=6) ZDF rats and loaded with Fluo-4AM and viewed under a confocal microscope to allow for the measurement of intracellular Ca2+ during and between transients. (otago.ac.nz)
  • Therefore the results from this study identify CaMKIIδ as a novel mediator of cardiac contractility in the type 2 diabetic heart, pre systolic dysfunction. (otago.ac.nz)
  • In the present study, we examined whether calcium/calmodulin-dependent protein kinase II (CaMKII) is affected by chronic developmental Pb2+ exposure. (cdc.gov)
  • The effects of Pb2+ exposure on rat hippocampal CaMKII were assessed by measuring CaMKII activity, phosphorylation of CaMKII at threonine-286, and CaMKII alpha and beta protein levels. (cdc.gov)
  • These Pb2+-induced changes in CaMKII activity could not be explained by changes in enzyme phosphorylation at threonine-286 or sensitivity to calmodulin. (cdc.gov)
  • Western blots of cytosolic and membrane fractions from hippocampus showed a significant decrease in the levels of CaMKII-beta but not alpha protein in the cytosolic fraction of Pb2+-exposed rats. (cdc.gov)
  • These findings indicate effects of developmental Pb2+ exposure on CaMKII, a component of calcium signaling associated with synaptic plasticity, learning, and memory. (cdc.gov)
  • A neuronal signaling pathway of CaMKII and Gqα regulates experience-dependent transcription of tph-1. (biomedsearch.com)
  • At the molecular level, the C. elegans homolog of CaMKII, UNC-43, functions cell-autonomously in ADF neurons to generate training-dependent enhancement in neuronal activity and tph-1 transcription, and this cell-autonomous function of UNC-43 is required for learning. (biomedsearch.com)
  • Ca 2+ /CaM-dependent protein kinase II (CaMKII) regulates Ca 2+ handling, and chronic overactivity of CaMKII is associated with left ventricular hypertrophy and dysfunction and lethal arrhythmias. (elsevier.com)
  • These CaMKII-induced changes of Na + channel gating were completely abolished by a specific CaMKII inhibitor, autocamtide-2-related inhibitory peptide (AIP). (elsevier.com)
  • On the other hand, cAMP-dependent protein kinase A inhibitors did not abolish the CaM/CaMKII-induced alterations of INa function.Conclusion Ca 2+ /CaM and CaMKII have distinct effects on the inactivation phenotype of cardiac Na + channels. (elsevier.com)
  • Ca(2+)/Calmodulin-dependent protein kinase II (CaMKII) signaling in the heart regulates cardiomyocyte contractility and growth in response to elevated intracellular Ca(2+). (escholarship.org)
  • CPCs undergoing early differentiation in vitro increase levels of CaMKIIδB in the nuclear compartment where the kinase may contribute to the regulation of CPC commitment. (escholarship.org)
  • Therefore, CaMKIIδB may serve as a novel modulatory protein to enhance CPC survival and commitment into the cardiac and smooth muscle lineages. (escholarship.org)
  • CaMKIIδ mediates β-adrenergic effects on RyR2 phosphorylation and SR Ca 2 + leak and the pathophysiological response to chronic β-adrenergic stimulation. (otago.ac.nz)
  • Dendrite formation involves activation of Ca2+/Calmodulin (CaM)-dependent kinase II (CaMKII) by CaM. (nih.gov)
  • Moreover, autophosphorylation of CaMKII and PKC was increased in presence of MEL, as well as phosphorylation of ERK1/2. (nih.gov)
  • Members of the CAMK enzyme class include, but are not limited to: CAMKI CAMKIα (CAMK1) CAMKIβ (PNCK) CAMKIδ CAMKIγ (CAMK1G) CAMKII CAMKIIα CAMKIIβ CAMKIIδ CAMKIIγ CAMKIII CAMKIV CAMKV CaM kinase like vesicle associated SCAMK Ca2+/calmodulin-dependent protein kinase kinase CAMKK1 CAMKK2 Pseudokinases are pseudoenzymes, proteins that resemble enzymes structurally, but lack catalytic activity. (wikipedia.org)
  • Beta-adrenergic (β-AR) tone is known to increase this leak through the activation of Ca-calmodulin-dependent protein kinase (CaMKII) and the subsequent phosphorylation of the ryanodine receptor. (elsevier.com)
  • When b-AR drive is chronic, as observed in heart failure, this CaMKII-dependent effect is exaggerated and becomes potentially arrhythmogenic. (elsevier.com)
  • We demonstrate that stimulation of the β-AR pathway by isoproterenol increased the CaMKII-dependent SR Ca 2+ leak. (elsevier.com)
  • In ventricular myocytes isolated from wild-type mice, isoproterenol stimulation also increased the CaMKII-dependent leak. (elsevier.com)
  • Aberrant calcium handling, including increased activation of calcium/calmodulin-dependent protein kinase II (CaMKII), contributes to arrhythmia development during HF. (elsevier.com)
  • Mice deficient for the gene encoding α-calcium-calmodulin-dependent kinase II (α-CaMKII knockout mice) provide a promising tool to link behavioral and cellular abnormalities with a specific molecular lesion. (elsevier.com)
  • The major binding species was a doublet at 50-60 kDa, which was identified as calcium/calmodulin-dependent protein kinase II (CaMKII) by N-terminal peptide analysis and confirmed by matrix-assisted laser desorption ionization-mass spectrometry as the δ- and β-like isoforms. (edu.au)
  • To investigate CaMKII involvement in insulin-stimulated glucose uptake, 3T3-L1 adipocytes were infected with retrovirus encoding green fluorescent protein (GFP)-hemagluttinin tag (HA)-tagged CaMKII wild-type or the ATP binding mutant, K42M. (edu.au)
  • GFP-HA-CaMKII K42M cells had less kinase activity than cells expressing wild-type GFP-HA-CaMKII. (edu.au)
  • Further quantitative immunoblotting indicated that CaMKIId is highly expressed in the vasculature and initial results suggest some alterations in ox-CaMKII protein levels with ageing, parallel to that observed in the heart. (strath.ac.uk)
  • A cultured macaque choroid-retinal endothelial cell line (RF/6A) was incubated in normal glucose (NG), NG plus the Ca 2+ entry blocker 2-aminoethoxydiphenyl borate (2-APB), high glucose (HG), or HG plus either 2-APB, the c-jun N-terminal kinase (JNK) inhibitor SP600125, or the calcium/calmodulin-dependent protein kinase II (CaMKII) inhibitor KN93. (molvis.org)
  • Expression levels of CaMKII, phosphorylated CaMKII (p-CaMKII), c-Jun N-terminal kinase (JNK), phosphorylated JNK (p-JNK), the death receptor (Fas), and cytochrome c were detected by western blotting analysis. (molvis.org)
  • HG-induced apoptosis in RF/6A cells depends on Ca 2+ entry and CaMKII activation, leading to the activation of both Fas-dependent and mitochondria-dependent apoptosis pathways. (molvis.org)
  • Recently, it was demonstrated that CaMKII is essential for both endoplasmic reticulum (ER) stress-induced apoptosis through the death receptor Fas, and for mitochondria-dependent apoptosis. (molvis.org)
  • Calcium/calmodulin-dependent protein kinase II (CaMKII) is upregulated in diabetes and significantly contributes to cardiac remodeling with increased risk of cardiac arrhythmias. (elsevier.com)
  • Activation of CaMKII occurs downstream of neurohormonal stimulation (e.g. via G-protein coupled receptors) and involve various posttranslational modifications including autophosphorylation, oxidation, S-nitrosylation and O-GlcNAcylation. (elsevier.com)
  • We also briefly summarize preclinical studies using kinase inhibitors and genetically modified mice targeting CaMKII in diabetes. (elsevier.com)
  • The enzyme Ca 2+ /calmodulin-dependent protein kinase II (CaMKII) is widely distributed in the nervous system. (utmb.edu)
  • Percentages of single-labeled L4 DRG neurons were 41% ± 2% CaMKIIα 38% ± 3% I-B4, 44% ± 3% CGRP, and 32% ± 6% VR1. (utmb.edu)
  • Percentages of single-labeled L5 DRG neurons were 44% ± 5% CaMKIIα, 48% ± 2% I-B4, 41% ± 7% CGRP, and 39% ± 14% VR1. (utmb.edu)
  • For L4 and L5, respectively, estimates of double-labeled CaMKIIα neurons showed 34% ± 2% and 38% ± 17% labeled for I-B4, 25% ± 14% and 19% ± 10% labeled for CGRP, and 37% ± 7% and 38% ± 5% labeled for VR1. (utmb.edu)
  • In our RyR2 R4496C+/- knock-in mouse model of CPVT we tested the hypothesis that inhibition of Ca 2+ /calmodulin-dependent protein kinase II (CaMKII) counteracts the effects of adrenergic stimulation resulting in an antiarrhythmic activity. (elsevier.com)
  • In ventricular myocytes isolated from the hearts of RyR2 R4496C+/- mice, CaMKII inhibition with an autocamtide-2 related inhibitory peptide or with KN-93 blunted triggered activity and transient inward currents induced by isoproterenol. (elsevier.com)
  • 5 Notably, this pathway could only be activated by Ca 2+ from local InsP 3 R (not global Ca 2+ transients), presumably because calmodulin and CaMKII physically associate with the InsP 3 R at the NucEn. (ahajournals.org)
  • Amphetamine exposure transiently increases Ca 2+ /calmodulin-dependent protein kinase II (CaMKII) α expression in the nucleus accumbens (NAcc) shell and this persistently increases local GluA1 S831 phosphorylation and enhances behavioral responding to the drug. (researchwithnj.com)
  • As expected, transient expression of CaMKIIα K42M in the NAcc shell produced a corresponding transient increase in CaMKIIα and decrease in pCaMKIIα (T286) protein levels in this site. (researchwithnj.com)
  • Whereas increased Ca 2+ /calmodulin-dependent protein kinase II (CaMKII) activity has been proposed to contribute to SR leak and AF induction, downstream targets of CaMKII remain controversial. (ahajournals.org)
  • To test the hypothesis that inhibition of CaMKII-phosphorylated type-2 ryanodine receptors (RyR2) prevents AF initiation in FKBP12.6-deficient (−/−) mice. (ahajournals.org)
  • 3 This has been attributed to increased S2808 phosphorylation on RyR2 by protein kinase A, 3 decreased levels of FK506-binding protein 12.6 (FKBP12.6) binding to RyR2, 3 and increased Ca 2+ /calmodulin-dependent protein kinase II (CaMKII) phosphorylation of RyR2 at S2814. (ahajournals.org)
  • We now show, through manipulation of intracellular [Ca2+] and the use of protein kinase inhibitors, that both protein kinase Calpha and Ca2+/calmodulin-dependent protein kinase II are involved in the phosphorylation of Tiam1 in vivo. (elsevier.com)
  • Targeting the ligand, VEGF, with humanized neutralizing monoclonal antibody, or the receptors with receptor tyrosine kinase inhibitors, are promising cancer therapy approaches [ 5 - 8 ]. (oncotarget.com)
  • In primary cortical neurons, BDNF elicited phosphorylation of AKT and p70S6 kinase is attenuated in the presence of myelin inhibitors. (jneurosci.org)
  • Furthermore, inhibition of JNK and the calcium signaling including the calmodulin-dependent protein kinase-II by either pharmacological inhibitors or their specific small interfering RNAs reversed CpG-induced protection against Vpr(52-96)-mediated apoptosis. (jimmunol.org)
  • Here, we describe in full detail the application of the method to identify targets of kinase inhibitors and immunophilin binders. (pnas.org)
  • The neurotrophin and GC-signaling pathways co-exist throughout the central nervous system (CNS), particularly in the hippocampus, which has high expression levels of glucocorticoid-receptors (GR) and mineralocorticoid-receptors (MR) as well as brain-derived neurotrophic factor (BDNF) and its receptor, tropomyosin-related kinase receptor B (TrkB). (frontiersin.org)
  • Angiotensin type 1a receptors in the forebrain subfornical organ facilitate leptin-induced weight loss through brown adipose tissue thermogenesis. (curehunter.com)
  • Furthermore, our findings indicate that nicotinic stimulation of β 2 -containing acetylcholine nicotinic receptors in the spinal cord can activate calcium/calmodulin-dependent calmodulin protein kinase II and produce nicotinic analgesia, which may require L-type calcium voltage and gated channels but not the intervention of glutamatergic transmission. (aspetjournals.org)
  • Also, PKC inhibition abolished dendritogenesis, while luzindole, an antagonist of MEL receptors (MT1/2), partially blocked the effects of MEL. (nih.gov)
  • Dendritogenesis elicited by MEL also required PKC activation, and signaling through MT1/2 receptors was partially involved. (nih.gov)
  • To evaluate the involvement of MEL receptors, hippocampal organotypic cultures were incubated with the MT1/2 receptor antagonist, luzindole. (nih.gov)
  • Stimulation of MEL receptors (MT1/2) causes PKC activation that has been shown to be involved in physiological responses such as phase shifts in circadian rhythms [39]. (nih.gov)
  • Spontaneous calcium waves in cardiac myocytes are caused by diastolic sarcoplasmic reticulum release (SR Ca 2+ leak) through ryanodine receptors. (elsevier.com)
  • PLC proteins can be activated by the receptor tyrosine kinases (RTK) or G-protein coupled receptors (GPCR) in response to the different extracellular stimuli including hormones and growth factors. (jcancer.org)
  • VEGF-A is the major angiogenic factor that activates VEGF receptors (VEGFR)-1 and -2 on endothelial cells. (oncotarget.com)
  • Mice lacking protein kinase Cε (PKCε) are supersensitive to positive allosteric modulators of gamma aminobutyrate type A (GABA A ) receptors. (pubmedcentralcanada.ca)
  • Evidence suggests that neural major histocompatibility complex class 1 (MHC1) molecules and their receptors function in activity-dependent synaptic plasticity and structural modification of synaptic connections ( Boulanger, 2009 ). (jneurosci.org)
  • 2 Previous studies revealed a higher open probability of type-2 ryanodine receptors (RyR2) isolated from dogs with chronic AF. (ahajournals.org)
  • Molecular signatures of G-protein-coupled receptors. (painresearchforum.org)
  • Lorecivivint, a Novel Intraarticular CDC-Like Kinase 2 and Dual-Specificity Tyrosine Phosphorylation-Regulated Kinase 1A Inhibitor and Wnt Pathway Modulator for the Treatment of Knee Osteoarthritis: A Phase II Randomized Trial. (escholarship.org)
  • This effect of calcium is completely blocked by exposure to a Ca 2+ /calmodulin (CaM)-dependent protein kinase (CaM-K) inhibitor ( 10 ) and thus suggests that the actions of calcium on the insulin gene are mediated in part by Ca 2+ /CaM-Ks. (diabetesjournals.org)
  • Since the CaM-K inhibitor blocked calcium stimulation of insulin gene transcription ( 10 ), this finding suggests the participation of CaM-Ks in glucose control of the gene. (diabetesjournals.org)
  • Chronic treatment with an L-type calcium channel blocker (nifedipine) or CaM kinase inhibitor (KN93) decreased dopamine transporter-mediated uptake and occluded the effects of tetrodotoxin and 4-aminopyridine. (elsevier.com)
  • Olomoucine is known as a cyclin-dependent kinase inhibitor. (edu.au)
  • Furthermore, HG increased JNK phosphorylation and Fas expression, and both responses were partially blocked by 2-APB and KN93, while the JNK inhibitor SP600125 partially reduced HG-induced Fas expression. (molvis.org)
  • Pretreatment with KN-93, a specific CaM kinase II inhibitor, attenuated both thrombin-induced increases in monolayer permeability to albumin and decreases in transendothelial electrical resistance (TER). (elsevier.com)
  • Herein we aimed to evaluate the antitumor and antiangiogenic potential of a novel pyrazolyl-urea kinase inhibitor, GeGe3, and to identify its kinase targets. (oncotarget.com)
  • The present study examined the effects of N- hydroxy- N ′-(4-butyl-2 methylphenyl) formamidine (HET0016), a selective inhibitor of the formation of 20-hydroxyeicosatrienoic acid (20-HETE) on the growth of 9L rat gliosarcoma cells in vitro and in vivo. (aspetjournals.org)
  • This was abolished by the Ca(2+)/calmodulin-dependent protein kinase II inhibitor KN93, suggesting that Ca(2+)/calmodulin-dependent protein kinase IIδ, a target of miR-145 and up-regulated in Dicer KO, plays a role in the regulation of L-type channel expression. (sigmaaldrich.com)
  • Moreover, CpG selectively induced the antiapoptotic cellular inhibitor of apoptosis (c-IAP)-2 protein and inhibition of the c-IAP-2 gene by either specific small interfering RNA or synthetic second mitochondrial activator of caspases mimetic reversed CpG-induced resistance against Vpr(52-96)-mediated apoptosis. (jimmunol.org)
  • Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis. (abcam.com)
  • The peptides RS20F and RS20CK correspond to CaM-binding amino acid sequence segments of either nonmuscle myosin light chain kinase (nmMLCK) or calmodulin-dependent protein kinase II (CaMPK-II), respectively. (elsevier.com)
  • Steady-state fluorescence intensity measurements indicated that, in the presence of an excess of calcium, both peptides bind to the calmodulin mutants with a 1:1 stoichiometry. (elsevier.com)
  • Both peptides induced similar changes in the fluorescence properties of the tryptophan residues located in the calcium-binding loops, with the exception of calmodulin with Trp-135. (elsevier.com)
  • Prendergast, Franklyn G. / Fluorescence analysis of calmodulin mutants containing tryptophan : Conformational changes induced by calmodulin-binding peptides from myosin light chain kinase and protein kinase II . (elsevier.com)
  • however, the mechanisms that underlie experience-dependent transcriptional regulation of the rate-limiting serotonin biosynthetic enzyme tryptophan hydroxylase (TPH) are poorly understood. (biomedsearch.com)
  • Upstream of ADF, the Gqα protein EGL-30 facilitates training-dependent induction of tph-1 by functional regulation of olfactory sensory neurons, which underscores the importance of sensory experience. (biomedsearch.com)
  • Multi-functional CAM Kinases have multiple targets they can phosphorylate and are found in processes including the secretion of neurotransmitters, metabolism of glycogen, and the regulation of various transcription factors. (wikipedia.org)
  • Calcium regulation of neuronal gene expression," Proceedings of the National Academy of Sciences of the United States of America , vol. 98, no. 20, pp. 11024-11031, 2001. (hindawi.com)
  • Up regulation of cAMP response element-mediated gene expression during experience-dependent plasticity in adult neocortex," Journal of Neuroscience , vol. 20, no. 11, pp. 4206-4216, 2000. (hindawi.com)
  • Padmanabhan, S , Lambert, NA & Prasad, BM 2008, ' Activity-dependent regulation of the dopamine transporter is mediated by Ca 2+ /calmodulin-dependent protein kinase signaling ', European Journal of Neuroscience , vol. 28, no. 10, pp. 2017-2027. (elsevier.com)
  • Klee CB, Ren H, Wang X (1998) Regulation of the calmodulin-stimulated protein phosphatase, calcineurin. (springer.com)
  • Garcia, Joe G N. / Regulation of endothelial cell barrier function by calcium/calmodulin-dependent protein kinase II . (elsevier.com)
  • Biochemical analysis of these kinases showed DMPK1 regulation upon VEGF challenge. (oncotarget.com)
  • The pathway maps illustrate protein interactions and regulation to provide a comprehensive picture of signaling and disease processes. (bio-rad.com)
  • We additionally identify regulation of the phosphorylation of several calcium and calmodulin-dependent protein kinases (CAMKs), including 2 sites of CAMK type II-gamma (CAMK2G), a protein known to regulate apoptosis. (diva-portal.org)
  • This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. (genecards.org)
  • Calcium/calmodulin-dependent protein kinase involved in the regulation of Ca(2+) homeostatis and excitation-contraction coupling (ECC) in heart by targeting ion channels, transporters and accessory proteins involved in Ca(2+) influx into the myocyte, Ca(2+) release from the sarcoplasmic reticulum (SR), SR Ca(2+) uptake and Na(+) and K(+) channel transport. (cusabio.com)
  • These results show that microRNAs play a crucial role in stretch-induced contractile differentiation in the vascular wall in part via miR-145-dependent regulation of L-type calcium channels. (sigmaaldrich.com)
  • Inhibition of calcium/calmodulin-dependent kinase II restores contraction and relaxation in isolated cardiac muscle from type 2 diabetic rats. (otago.ac.nz)
  • S -nitrosylation induces both autonomous activation and inhibition of calcium/calmodulin-dependent protein kinase II δ. (otago.ac.nz)
  • Conversely, TNF-α- or SeV-induced type I interferon response increased when sorcin knocked down, leading to inhibition of vesicular stomatitis virus replication. (frontiersin.org)
  • This suggests that NgR1 and PirB participate in ligand-dependent inhibition of synaptic plasticity. (jneurosci.org)
  • Basal and stretch-induced L-type calcium channel expressions were both decreased in Dicer KO portal veins, and inhibition of L-type channels in control vessels mimicked the effects of Dicer deletion. (sigmaaldrich.com)
  • Furthermore, inhibition of miR-145, a highly expressed microRNA in smooth muscle, resulted in a similar reduction of L-type calcium channel expression. (sigmaaldrich.com)
  • By focusing on specific protein classes (e.g., kinases), this paradigm of drug discovery routinely uses in vitro assays with recombinant proteins in binding or biochemical assays (refs. (pnas.org)
  • Aims Calmodulin (CaM) regulates Na + channel gating through binding to an IQ-like motif in the C-terminus. (elsevier.com)
  • Leptin receptor signaling in the hypothalamus regulates hepatic autonomic nerve activity via phosphatidylinositol 3-kinase and AMP-activated protein kinase. (curehunter.com)
  • Ca2+/calmodulin-dependent protein kinase II delta2 regulates gene expression of insulin in INS-1 rat insulinoma cells. (semanticscholar.org)
  • Exton, J H. / Ca2+/calmodulin-dependent protein kinase II regulates Tiam1 by reversible protein phosphorylation . (elsevier.com)
  • Spontaneous calcium activity in metanephric mesenchymal cells regulates branching morphogenesis in the embryonic kidney. (diva-portal.org)
  • Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. (genecards.org)
  • To do this a mouse model of type 2 diabetes (db/db) (n=8) alongside nDM control mice (n=11) were injected with KN93 (10 μmol/kg) every other day from the age of 14 to 18 weeks of age. (otago.ac.nz)
  • Neuronal receptor activity-modifying protein 1 promotes energy expenditure in mice. (curehunter.com)
  • The antinociceptive effects of i.t. nicotine in the tail-flick test did not significantly differ in wild-type and α 7 knockout (KO) animals but were lost in β 2 knockout mice. (aspetjournals.org)
  • When calcium/calmodulin-dependent calmodulin protein kinase II activity in the lumbar spinal cord after acute i.t. administration of nicotine was investigated in wild-type and β 2 and α 7 knockout mice, the increase in calcium/calmodulin-dependent calmodulin protein kinase II activity was not significant reduced in α 7 KO mice but was eliminated in the β 2 KO mice. (aspetjournals.org)
  • Importantly, splenocytes from sorcin −/− mice produced more IL-2, IL-4, IL-17, and IFN-γ than that of littermate controls (sorcin +/+ ) in response to anti-CD3/28 stimulation. (frontiersin.org)
  • Measurements of nuclear and cytosolic Ca 2+ transients in IP 3 sponge mice showed no increase of Ca 2+ levels during diastole as we detected in wild-type mice. (springer.com)
  • Nuclei, isolated from ventricular myocytes of mice after chronic Ang II treatment, showed an elevation of IP 3 R2 expression which was dependent on calcineurin/NFAT signaling and persisted for 3 weeks after removal of the Ang II stimulus. (springer.com)
  • Treatment of PKCε-null mice with the GABA A receptor antagonist bicuculline restored corticosterone levels and anxiety-like behavior to wild-type levels. (pubmedcentralcanada.ca)
  • Moreover, several single gene mutations alter anxiety-like behavior in mice, including null mutations in the corticotropin-releasing factor (CRF) receptor-1 ( 7 , 8 ), the glucocorticoid receptor ( 9 ), the α subunit of calcium/calmodulin-dependent protein kinase II ( 10 ), and protein kinase Cγ (PKCγ) ( 11 ), which all reduce anxiety-related behaviors. (pubmedcentralcanada.ca)
  • In contrast, null mutations in glutamate decarboxylase (GAD) 65 ( 12 ), which synthesizes gamma aminobutyrate (GABA), the GABA type A (GABA A ) receptor γ2 subunit ( 13 ), CRF receptor-2 ( 14 , 15 ), and the serotonin 5-HT 1A receptor ( 16 - 18 ) increase anxiety-like behavior in mice. (pubmedcentralcanada.ca)
  • These mice are similar in length and weight to wild-type littermates, behave normally in their home cage, and show no compensatory changes in the abundance of other PKC isozymes in the nervous system ( 20 , 21 ). (pubmedcentralcanada.ca)
  • In acute hippocampal slices of adult mice, Nogo-66 and OMgp suppress NMDA receptor-dependent long-term potentiation (LTP) when locally applied to Schaffer collateral-CA1 synapses. (jneurosci.org)
  • Shibata, "Mutant mice lacking ryanodine receptor type 3 exhibit deficits of contextual fear conditioning and activation of calcium/calmodulin-dependent protein kinase II in the hippocampus," Brain Research. (thefreedictionary.com)
  • Mice lacking RyR2-stabilizing subunit FKBP12.6 had a higher incidence of spontaneous and pacing-induced AF compared with wild-type mice. (ahajournals.org)
  • See 5 reference sequence protein isoforms for the CAMK2A gene. (nih.gov)
  • p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence. (uniprot.org)
  • 7. entry: EMBL:DMVASA2 DE Drosophila melanogaster vasa gene segment 2 (exons 3 to 7) 8. (bio.net)
  • Cyclin-dependent kinase 5 activator 2 is an enzyme that in humans is encoded by the CDK5R2 gene. (wikipedia.org)
  • The protein encoded by this gene is a neuron-specific activator of CDK5 kinase. (wikipedia.org)
  • Protein kinase C and calcium/calmodulin-activated protein kinase II (CaMK II) suppress nicotinic acetylcholine receptor gene expression in mammalian muscle. (elsevier.com)
  • Carlton, SM & Hargett, GL 2002, ' Stereological analysis of Ca 2+ /calmodulin-dependent protein kinase IIα-containing dorsal root ganglion neurons in the rat: Colocalization with isolectin Griffonia Simplicifolia, calcitonin gene-related peptide, or vanilloid receptor 1 ', Journal of Comparative Neurology , vol. 448, no. 1, pp. 102-110. (utmb.edu)
  • Hargett, Gregory L. / Stereological analysis of Ca 2+ /calmodulin-dependent protein kinase IIα-containing dorsal root ganglion neurons in the rat : Colocalization with isolectin Griffonia Simplicifolia, calcitonin gene-related peptide, or vanilloid receptor 1 . (utmb.edu)
  • The product of this gene belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. (genecards.org)
  • CAMK4 (Calcium/Calmodulin Dependent Protein Kinase IV) is a Protein Coding gene. (genecards.org)
  • GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity . (genecards.org)
  • Purified Pak1 kinase promotes the phosphorylation of the Snf1 polypeptide on threonine 210 within the activation loop in vitro, and an increased dosage of the PAK1 gene causes increased Snf1 threonine 210 phosphorylation in vivo. (asm.org)
  • Deletion of the PAK1 gene does not produce a Snf phenotype, suggesting that one or more additional protein kinases is able to activate Snf1 in vivo. (asm.org)
  • Prion Protein Gene M129V Polymorphism and Variability in Age at Migraine Onset. (painresearchforum.org)
  • Both gene sets were significantly enriched in predicted secreted proteins, including potential signaling components and proteins that might contribute to reinforcing, modifying, or remodeling the structure of the extracellular matrix during pollination. (plantphysiol.org)
  • However, both types of cells are difficult to isolate by conventional microdissection methods in the numbers required for gene expression profiling. (plantphysiol.org)
  • The abundantly expressed calcium/calmodulin-dependent protein kinase II (CAMK2), alpha (CAMK2A), and beta (CAMK2B) isoforms are essential for learning and memory formation. (eur.nl)
  • We have identified a significantly narrower SC in oocytes and have established that this difference does not arise from a different organization of the lateral elements nor from a different isoform of transverse filament protein SYCP1. (diva-portal.org)
  • The altered neuronal activity caused by these two drugs is accompanied by changes in intracellular calcium concentrations and Ca 2+ /calmodulin-dependent protein (CaM) kinase II activity in dopamine neurons. (elsevier.com)
  • These kinases belong to a diverse group of enzymes that participate in many cellular responses and are activated by increasing concentrations of intracellular Ca 2+ . (diabetesjournals.org)
  • Previous work showed that MEL increased the synthesis and translocation of CaM, suggesting that MEL activates CaM-dependent enzymes by this pathway. (nih.gov)
  • CAMK, also written as CaMK, is an abbreviation for the Ca2+/calmodulin-dependent protein kinase class of enzymes. (wikipedia.org)
  • When activated, the enzymes transfer phosphates from ATP to defined serine or threonine residues in other proteins, so they are serine/threonine-specific protein kinases. (wikipedia.org)
  • Although importance of PLC enzymes for key cellular functions is well established, the PLC proteins belonging to the ε, ζ and η subfamilies were identified and characterized only during the last decade. (jcancer.org)
  • This study aims to elucidate Ca 2+ -dependent signaling mechanisms that mediate hyperglycemia-induced apoptosis in RECs. (molvis.org)
  • Enhanced apoptosis was blocked by 2-APB and KN93. (molvis.org)
  • These early signs of mitochondria-dependent apoptosis were partially reversed by 2-APB and KN93. (molvis.org)
  • Subsaturating concentration of ouabain triggers intracellular calcium oscillations, stimulates cell proliferation and adhesion, and protects from apoptosis. (diva-portal.org)
  • CAMK2G knockdown impaired ouabain-dependent protection from apoptosis upon treatment with high glucose or serum deprivation. (diva-portal.org)
  • After 48 h of incubation, HET0016 reduced the proliferation of 9L in vitro by 55%, and this was associated with a fall in p42/p44 mitogen-activated protein kinase and stress-activated protein kinase/c-Jun NH 2 -terminal kinase phosphorylation and increased apoptosis. (aspetjournals.org)
  • Using HIV-viral protein R (Vpr)(52-96) peptide as a model apoptosis-inducing agent, we demonstrated that unlike monocyte-derived macrophages, undifferentiated primary human monocytes and promonocytic THP-1 cells are highly susceptible to Vpr(52-96)-induced apoptosis. (jimmunol.org)
  • The budding yeast Snf1 kinase and mammalian AMPK are orthologous proteins, and they play similar roles in controlling cellular metabolism. (asm.org)
  • Calcium/calmodulin-dependent protein kinase II activity and expression are altered in the hippocampus of Pb2+- exposed rats. (cdc.gov)
  • This review addresses the effects of ELS paradigms on GC- and BDNF-dependent mechanisms and their crosstalk in the hippocampus, including potential implications for the pathogenesis of common stress-related disorders. (frontiersin.org)
  • In this mini-review we present the effects of ELS on GC- and BDNF-dependent mechanisms in the hippocampus using primarily evidence from ELS animal models. (frontiersin.org)
  • Melatonin stimulates dendrite formation and complexity in the hilar zone of the rat hippocampus: participation of the Ca++/Calmodulin complex. (nih.gov)
  • abstract = "A number of guanine nucleotide exchange factors have been identified that activate Rho family GTPases, by promoting the binding of GTP to these proteins. (elsevier.com)
  • abstract = "Calcium/calmodulin-dependent protein kinase II delta (CaMKIId) plays a fundamental role in cardiac dysfunction and is known to be overexpressed and hyper-activated in the diseased heart. (strath.ac.uk)
  • Peptide-induced conformational changes in five isofunctional mutants of calmodulin (CaM), each bearing a single tryptophan residue either at the seventh position of each of the four calcium-binding loops (i.e., amino acids 26, 62, 99, and 135) or in the central helix (amino acid 81) were studied by using fluorescence spectroscopy. (elsevier.com)
  • Whole-cell, voltage-clamp experiments were performed in human endothelial kidney 293 cells coexpressing wild-type or mutant KCNQ1 and KCNE1 (auxiliary subunit) during ISO treatment or lentiviral δCaMKII overexpression. (elsevier.com)
  • Prolonged hyperglycemia is known to increase intracellular cytosolic free calcium ([Ca 2+ ]i) in retinal vascular endothelial cells (RECs), suggesting that [Ca 2+ ]i is a critical trigger for microvascular degeneration. (molvis.org)
  • Thrombin-induced endothelial cell barrier dysfunction is tightly linked to Ca 2+ -dependent cytoskeletal protein reorganization. (elsevier.com)
  • Together, these studies indicate that CaM kinase II activation and filamin phosphorylation may participate in thrombin-induced cytoskeletal reorganization and endothelial barrier dysfunction. (elsevier.com)
  • Tumor angiogenesis depends on angiogenic factors and the transduction of their signals in endothelial cells [ 2 ]. (oncotarget.com)
  • Extensive expression of Vascular Endothelial Growth Factor (VEGF)-A during tumor growth was reported for several tumor types [ 3 , 4 ]. (oncotarget.com)
  • Elevated glucose concentrations stimulate the transcription of the insulin and several other genes that regulate glucose homeostasis ( 1 , 2 ). (diabetesjournals.org)
  • Human studies have detected associations between panic disorder and polymorphisms in genes for MAO-A ( 2 ), the adenosine A2a receptor ( 3 ), and cholecystokinin ( 4 , 5 ). (pubmedcentralcanada.ca)
  • In the nucleus, phosphorylates the MEF2 repressor HDAC4, promoting its nuclear export and binding to 14-3-3 protein, and expression of MEF2 and genes involved in the hypertrophic program. (cusabio.com)
  • In order to identify these genes, we used the Arabidopsis ( Arabidopsis thaliana ) ATH1 microarray to compare the whole-genome transcriptional profiles of stigmas and ovaries isolated from wild-type Arabidopsis and from transgenic plants in which cells of the stigma epidermis and transmitting tract were specifically ablated by expression of a cellular toxin. (plantphysiol.org)
  • Silencing the catalytic site of the CAMK2G p.Arg292Pro protein reversed the pathogenic effect of the p.Arg292Pro mutation on neuronal maturation, without rescuing its nuclear targeting. (eur.nl)
  • These data suggest that neuronal activity can regulate dopamine transporter function and abundance via calcium/CaM kinase II signaling. (elsevier.com)
  • Loss of NgR1 leads to increased phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2), signaling intermediates known to regulate neuronal growth and synaptic function. (jneurosci.org)
  • Early in postnatal development a number of refinement processes sculpt neuronal connectivity in an experience-dependent manner. (jneurosci.org)
  • Beyond the CP, use-dependent changes in neuronal connectivity are less pronounced. (jneurosci.org)
  • Components of the Nogo receptor (NgR1) complex have been implicated in activity-dependent refinement of neuronal connectivity in the healthy CNS. (jneurosci.org)
  • Over the past 15 years or so, numerous studies have sought to characterise how nuclear calcium (Ca 2+ ) signals are generated and reversed, and to understand how events that occur in the nucleoplasm influence cellular Ca 2+ activity, and vice versa. (biologists.org)
  • As a largest known PLC protein, PLCε is involved in a variety of signaling pathways and controls different cellular properties. (jcancer.org)
  • IP3 stimulates Ca 2+ signaling and DAG acts through protein kinase C (PKC) leading to different cellular events, such as proliferation, growth and migration. (jcancer.org)
  • Our previous report showed that foot-and-mouth disease virus (FMDV) VP1 inhibited TNF-α- and SeV-induced type I interferon response via interaction with cellular protein soluble resistance-related calcium-binding protein (sorcin). (frontiersin.org)
  • Our recent publication shows that VP1 inhibits TNF-α-induced activation of NF-κB and type I interferon promoters via interaction with cellular protein soluble resistance-related calcium-binding protein (sorcin) ( 3 ). (frontiersin.org)
  • Our previous report showed that sorcin inhibited TNF-α- or SeV-induced activation of type I interferon and NF-κB promoters ( 3 ), revealing a novel role of sorcin as an important cellular regulator for host response. (frontiersin.org)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Hierarchical clustering revealed that ouabain triggers a structured phosphorylation response that occurs in a well-defined, time-dependent manner and affects specific cellular processes, including cell proliferation and cell-cell junctions. (diva-portal.org)
  • Martinez,IM, Chrispeels,MJ: Genomic analysis of the unfolded protein response in Arabidopsis shows its connection to important cellular processes. (amazonaws.com)
  • 2. The Institute of Molecular Biology and Genetics of NASU, Kyiv, Ukraine. (jcancer.org)
  • From the standpoint of drug safety and efficacy, unbiased identification of proteins and associated molecular complexes that bind to a drug allows direct evaluation of its polypharmacology ( 4 ) and provides valuable insight into its mode of action and avenues for compound optimization. (pnas.org)
  • Furthermore, we show that Ca2+/calmodulin-dependent protein kinase II phosphorylates Tiam1 in vitro, producing an electrophoretic retardation on SDS-polyacrylamide gel electrophoresis. (elsevier.com)
  • Furthermore, Tiam1 was preferentially dephosphorylated by protein phosphatase 1 in vitro, and treatment with this phosphatase abolished the Ca2+/calmodulin-dependent protein kinase II activation of Tiam1. (elsevier.com)
  • These data demonstrate that protein kinase Calpha and Ca2+/calmodulin-dependent protein kinase II phosphorylate Tiam1 in vivo, and that the latter kinase plays a key role in regulating the activity of this exchange factor in vitro. (elsevier.com)
  • Snf1 kinase complexes isolated from pak1 Δ mutant strains show reduced specific activity in vitro, and affinity-purified Pak1 kinase is able to activate the Snf1-dependent phosphorylation of Mig1 in vitro. (asm.org)
  • The present study was undertaken to investigate the hypothesis that the β 2 nicotinic receptor subunit plays a central role in nicotine-induced spinal antinociception via calcium/calmodulin-dependent calmodulin protein kinase II activation. (aspetjournals.org)
  • Fingerprint Dive into the research topics of 'Calcium/calmodulin-dependent protein kinase II - does ageing mirror disease? (strath.ac.uk)
  • There are two multifunctional CaM-Ks, named CaM-KI and -IV, which are activated by an upstream CaM-K kinase (CaM-KK) through phosphorylation of a threonine residue within the active loop of the protein. (diabetesjournals.org)
  • Therefore, Snf1 kinase must be activated by an upstream kinase. (asm.org)
  • Analysis of the tryptophan fluorescence decay of the last mentioned calmodulins supports a model in which the equilibrium between two (Trp-99) or three (Trp-62) states of these tryptophan residues, each characterized by a different lifetime, was altered toward the blue-shifted short lifetime component upon peptide binding. (elsevier.com)
  • Fluorescence recovery after photobleach (FRAP) was measured using the low-affinity Ca 2+ indicator Fluo-5N trapped inside the SR and NucEn (where its fluorescence is high because [Ca 2+ ] is &1 mmol/L). After Fluo-5N photobleach in one end of the cell, FRAP occurred, accompanied by fluorescence decline in the unbleached end with similar time constants (τ&2 minutes) until fluorescence regained spatial uniformity. (ahajournals.org)
  • Taken together, these results are consistent with the hypothesis that increases in intracellular calcium result in activation of calcium-mediated second messengers in the spinal cord that play an important role in nicotine-induced antinociception as measured in the tail-flick test. (aspetjournals.org)
  • CAMKs are activated by increases in the concentration of intracellular calcium ions (Ca2+) and calmodulin. (wikipedia.org)
  • CAMK1D encodes a member of the Ca2+/calmodulin-dependent protein kinase 1 subfamily of serine/threonine kinases. (antikoerper-online.de)
  • DAP kinase subfamily. (abcam.com)
  • It differs from other enzyme subtypes in that it lacks a phosphorylatable activation domain that can respond to CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASE KINASE. (nih.gov)
  • In this study, we examined the role of the Ca 2+ /calmodulin (CaM)-dependent protein kinase (CaM-K) cascade in transcriptional activation of insulin. (diabetesjournals.org)
  • Involvement of hypothalamic AMP-activated protein kinase in leptin-induced sympathetic nerve activation. (curehunter.com)
  • Figure 1 shows how the presence of calcium or calmodulin allows for the activation of CAM kinases (CAMK II). (wikipedia.org)
  • This leads to the activation of protein kinase C and D and subsequent downstream signaling: PKC induces phosphorylation of multiple transcription factors and along with DAG takes part in Ca2+ signaling (Figure 1 ). (jcancer.org)
  • Lines of evidence showed that negative regulating proteins played an essential role in homeostasis, such as suppressors of SOCS proteins as counterparts for STAT ( 4 , 5 ), Mdm2 as negative regulator for p53 ( 6 - 8 ), and CTLA4 as a brake for TCR-mediated T cell activation ( 9 - 11 ). (frontiersin.org)
  • Zhu J, McKeon F (1999) NF-AT activation requires suppression of Crm1-dependent export by calcineurin. (springer.com)
  • The limitation of targeting VEGF/VEGFR2 signalling to stop angiogenesis in cancer therapy has been blamed on re-activation of alternative receptor tyrosine kinases by compensatory angiogenic factors. (oncotarget.com)
  • Recent studies of AMPK underscore its importance as a metabolic switch and suggest that activation of AMPK may provide a therapeutic benefit for patients with type 2 diabetes ( 38 ). (asm.org)
  • Biochemical and genetic experiments have shown that members of the Snf1/AMP-activated protein kinase family are regulated by phosphorylation of the conserved threonine residue in the kinase activation loop. (asm.org)
  • By using this antibody, we have shown that phosphorylation of Snf1 threonine 210 correlates with glucose stress and Snf1 kinase activation. (asm.org)
  • A protein called activation-induced deaminase (AID) plays an essential role in the development of B-cell lymphomas such as non-Hodgkin's lymphoma. (westonaprice.org)
  • 1998 ) reported that activation of the Ras/MAPK by norepinephrine, angiotensin II, and EGF is dependent on the formation of 20-HETE, which is generated after activation of phospholipase A 2 by calcium/calmodulin protein kinase II. (aspetjournals.org)
  • Stretch of the vascular wall is an important stimulus to maintain smooth muscle contractile differentiation that is known to depend on L-type calcium influx, Rho-activation, and actin polymerization. (sigmaaldrich.com)
  • We also show that CpG induced JNK phosphorylation through activation of the calcium signaling pathway. (jimmunol.org)
  • Substrate-specific CAM Kinases only have one target that they can phosphorylate, such as myosin light chain kinases. (wikipedia.org)
  • We next explored potential thrombin-induced CaM kinase II cytoskeletal targets and found that thrombin causes translocation and significant phosphorylation of nonmuscle filamin (ABP-280), which was attenuated by KN-93, whereas thrombin-induced myosin light chain phosphorylation was unaffected. (elsevier.com)
  • The CAM Kinase contains a highly concentrated glycine loop where the gamma phosphate from the donor ATP molecule is easily able to bind to the enzyme which then utilizes the metal ion to facilitate a smooth phosphate transfer to the target protein. (wikipedia.org)
  • Biochemical fractionation of rat liver has shown that threonine 172 of the mammalian AMPK enzyme is phosphorylated by a distinct protein kinase called AMPK kinase (AMPKK) ( 11 ). (asm.org)
  • A CALMODULIN-dependent enzyme that catalyzes the phosphorylation of proteins. (harvard.edu)
  • This enzyme is also sometimes dependent on CALCIUM. (harvard.edu)
  • Exposure of INS-1 cells to 11.2 mmol/l glucose elicited an increase of insulin promoter activity as well as upregulation of CaM-KIV activity within 2 min after stimulation. (diabetesjournals.org)
  • Pak1 associates with the Snf1 kinase in vivo, and the association is greatly enhanced under glucose-limiting conditions when Snf1 is active. (asm.org)
  • Nutrient-sensing pathways, in particular, glucose-sensing pathways, are critical signaling pathways that are deregulated in type 2 diabetes. (asm.org)
  • Among its related pathways are Calcium signaling pathway and DAG and IP3 signaling . (genecards.org)
  • We demonstrated that c-IAP-2 is regulated by the JNK and calcium signaling pathway, in particular calmodulin-dependent protein kinase-II. (jimmunol.org)
  • Furthermore, a cell-permeable N-myristoylated synthetic filamin peptide (containing the COOH-terminal CaM kinase II phosphorylation site) attenuated both thrombin-induced filamin phosphorylation and decreases in TER. (elsevier.com)
  • CaM-KIVc alone was sufficient, and the upstream kinase, CaM-KK, was enhanced to upregulate the insulin promoter activity in INS-1 cells. (diabetesjournals.org)
  • Members of the Snf1/AMP-activated protein kinase family are activated under conditions of nutrient stress by a distinct upstream kinase. (asm.org)
  • Type 2 diabetes is a well-established risk factor for cardiovascular disease, with the landmark Framingham study in the 1970's reporting the incidence of heart failure is significantly higher in diabetic (DM) patients compared to non-diabetic (nDM) patients. (otago.ac.nz)
  • However the underlying mechanisms that link type 2 diabetes with heart failure are still unclear. (otago.ac.nz)
  • Cross-sectional and Prospective Associations of Rest-Activity Rhythms With Metabolic Markers and Type 2 Diabetes in Older Men. (escholarship.org)
  • Carvedilol and metoprolol are both able to preserve myocardial function in type 2 diabetes. (otago.ac.nz)
  • A significant association of rs10906115 in CDC123 (zeige CDC123 Antikörper )/ CAMK1D and rs1359790 near SPRY2 (zeige SPRY2 Antikörper ) was identified with type 2 diabetes in a Japanese population. (antikoerper-online.de)
  • Espino-Paisan L, Urcelay E, Concha EGdl, Santiago JL: Early and Late Onset Type 1 Diabetes: One and the Same or Two Distinct Genetic Entities? (amazonaws.com)
  • Zhong J, Xu J, Yang P, Liang Y, Wang C-Y: Innate immunity in the recognition of beta-cell antigens in type 1 diabetes. (amazonaws.com)
  • Type 1 and type 2 diabetes each have similarities in that both types of diabetes involve insulin from the pancreas not working properly as it should. (amazonaws.com)
  • Many people with type 2 diabetes start out by diet and exercise, trying to lower their weight to improve their sugars before trying any oral anti-diabetic medications. (amazonaws.com)
  • Type 1 diabetes, once it is discovered, will need to take insulin right away in order to live, otherwise they will die without it if not taken when they are supposed to. (amazonaws.com)
  • Understanding the Effects of Roux-en-Y Gastric Bypass (RYGB) Surgery on Type 2 Diabetes MellitusRaymond G. Why there is this discordant finding between HOMA-IR measures and insulin clamp studies is unclear. (amazonaws.com)
  • Adoption of new diagnostic criteria recommended by the American Diabetes Association (ADA) [1] , [2] estimates a global prevalence of nearly one in five women (∼18%) who are considered at risk for GDM. (plos.org)
  • This mechanism for controlling protein kinase activity is used both by members of the serine threonine protein kinase family and by members of the tyrosine protein kinase family. (asm.org)
  • In addition, L-type calcium channel blockers nimodipine and verapamil but not the N -methyl- d -aspartate antagonist MK-801 (dizocilpine maleate) blocked the increase in the kinase activity induced by nicotine. (aspetjournals.org)
  • None of the mutants exhibited enhanced Ca 2+ -independent kinase activity toward exogenous substrate, but the K300S and N294S mutants showed a significant enhancement in the rate and stoichiometry of 32 P incorporation during Ca 2+ -independent autophosphorylation. (elsevier.com)
  • Taken together, our results reveal an indispensable function of CAMK2G in neurodevelopment and indicate that the CAMK2G p.Arg292Pro protein acts as a pathogenic gain-of-function mutation, through constitutive activity toward cytosolic targets, rather than impaired targeting to the nucleus. (eur.nl)
  • Significantly, phosphorylation of Tiam1 by Ca2+/calmodulin-dependent protein kinase II, but not by protein kinase C, enhanced its nucleotide exchange activity toward Rac1, by approximately 2-fold. (elsevier.com)
  • It did so without affecting the activity of known insulin signaling proteins. (edu.au)
  • In this study, we found that thrombin increased Ca 2+ /calmodulin-dependent protein kinase II (CaM kinase II) activities in a Ca 2+ - and time-dependent manner in bovine pulmonary endothelium with maximal activity at 5 min. (elsevier.com)
  • Follow-up analyses showed reduced enzymatic activity of mitochondrial complexes C-I, C-III, and C-IV (−60-75%) in the OGDM (n = 8) compared with ONGT (n = 10) subjects, though no differences were observed for mitochondrial complex protein content. (plos.org)
  • Recent studies have implicated the involvement of Ca 2+ -dependent mechanisms, in particular, calcium/calmodulin-protein kinase II in nicotine-induced antinociception using the tail-flick test. (aspetjournals.org)
  • We have recently demonstrated that lysophosphatidic acid and several other agonists stimulate phosphorylation of the Rac1-specific exchange factor Tiam1 in Swiss 3T3 fibroblasts, and that protein kinase C is involved in Tiam1 phosphorylation (Fleming, I. N., Elliott, C. M., Collard, J. G., and Exton, J. H. (1997) J. Biol. (elsevier.com)
  • Figure 6 shows images of hippocampal slices cultured with the MT1/2 receptor antagonist in presence of either the vehicle or MEL and stained with the MAP2 antibody. (nih.gov)
  • More recently, we developed a phosphopeptide antibody that is specific for the Snf1 protein that has been phosphorylated on threonine 210 ( 21 ). (asm.org)
  • Ca(2+)/calmodulin-dependent protein kinase II is highly expressed in insulin secreting cells and is associated with insulin secretory granules and has been proposed to play an important role in exocytosis or in insulin granule transport to release sites. (semanticscholar.org)
  • Transcripts of calcium/calmodulin-dependent kinases are changed after forskolin- or IBMX-induced insulin secretion due to melatonin treatment of rat insulinoma beta-cells (INS-1). (semanticscholar.org)
  • Taken together, our results suggest that CpG-induced protection may be mediated by c-IAP-2 through the calcium-activated JNK pathway via what appeared to be TLR9-independent signaling pathways. (jimmunol.org)
  • Immunostimulatory RNAs (like double stranded RNAs) generated through deregulated RNA processing pathways along with RNA binding proteins (RBPs) of RNA helicase (RNA sensors) family are emerging as important components of immune response pathways during sterile inflammation. (intechopen.com)
  • CAMK II is the main protein in this subset. (wikipedia.org)
  • Figure 2 goes into detail showing the structure and domains of CAMK I. CAMK II has a variety of different forms, with CAMK 2A being the most common, as shown in Figure 3. (wikipedia.org)
  • 1 Electrical depolarization of the cell membrane opens L-type Ca 2+ channels, causing Ca 2+ influx. (ahajournals.org)
  • The channels are normally sensitive to calcium ions and not sensitive to inositol triphosphate. (thefreedictionary.com)
  • MicroRNAs are essential for stretch-induced vascular smooth muscle contractile differentiation via microRNA (miR)-145-dependent expression of L-type calcium channels. (sigmaaldrich.com)
  • On the other hand, acute stretch-sensitive growth signaling, which is independent of influx through L-type calcium channels, was not affected by Dicer KO. (sigmaaldrich.com)
  • A biologic function for an "orphan" messenger: D-myo-inositol 3,4,5,6-tetrakisphosphate selectively blocks epithelial calcium-activated chloride channels. (ac.be)