A calbindin protein found in many mammalian tissues, including the UTERUS, PLACENTA, BONE, PITUITARY GLAND, and KIDNEYS. In intestinal ENTEROCYTES it mediates intracellular calcium transport from apical to basolateral membranes via calcium binding at two EF-HAND MOTIFS. Expression is regulated in some tissues by VITAMIN D.
Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS.
Calcium-binding proteins that are found in DISTAL KIDNEY TUBULES, INTESTINES, BRAIN, and other tissues where they bind, buffer and transport cytoplasmic calcium. Calbindins possess a variable number of EF-HAND MOTIFS which contain calcium-binding sites. Some isoforms are regulated by VITAMIN D.
A calcium-binding protein that mediates calcium HOMEOSTASIS in KIDNEYS, BRAIN, and other tissues. It is found in well-defined populations of NEURONS and is involved in CALCIUM SIGNALING and NEURONAL PLASTICITY. It is regulated in some tissues by VITAMIN D.
A calbindin protein that is differentially expressed in distinct populations of NEURONS throughout the vertebrate and invertebrate NERVOUS SYSTEM, and modulates intrinsic neuronal excitability and influences LONG-TERM POTENTIATION. It is also found in LUNG, TESTIS, OVARY, KIDNEY, and BREAST, and is expressed in many tumor types found in these tissues. It is often used as an immunohistochemical marker for MESOTHELIOMA.
Low molecular weight, calcium binding muscle proteins. Their physiological function is possibly related to the contractile process.
A family of highly acidic calcium-binding proteins found in large concentration in the brain and believed to be glial in origin. They are also found in other organs in the body. They have in common the EF-hand motif (EF HAND MOTIFS) found on a number of calcium binding proteins. The name of this family derives from the property of being soluble in a 100% saturated ammonium sulfate solution.
Calcium-binding motifs composed of two helices (E and F) joined by a loop. Calcium is bound by the loop region. These motifs are found in many proteins that are regulated by calcium.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
A heat-stable, low-molecular-weight activator protein found mainly in the brain and heart. The binding of calcium ions to this protein allows this protein to bind to cyclic nucleotide phosphodiesterases and to adenyl cyclase with subsequent activation. Thereby this protein modulates cyclic AMP and cyclic GMP levels.
A neuronal calcium-sensor protein that is found in ROD PHOTORECEPTORS and CONE PHOTORECEPTORS. It interacts with G-PROTEIN-COUPLED RECEPTOR KINASE 1 in a Ca2+ dependent manner and plays an important role in PHOTOTRANSDUCTION.
A 13.2-kDa member of the S-100 family of calcium-binding proteins that can form homo- or heterocomplexes with CALGRANULIN A and a variety of other proteins. The calgranulin A/B heterodimer is known as LEUKOCYTE L1 ANTIGEN COMPLEX. Calgranulin B is expressed at high concentrations in GRANULOCYTES during early monocyte differentiation, and serum calgranulin B levels are elevated in many inflammatory disorders such as CYSTIC FIBROSIS.
Acidic protein found in SARCOPLASMIC RETICULUM that binds calcium to the extent of 700-900 nmoles/mg. It plays the role of sequestering calcium transported to the interior of the intracellular vesicle.
A 10.8-kDa member of the S-100 family of calcium-binding proteins that can form homo- or heterocomplexes with CALGRANULIN B and a variety of other proteins. The calgranulin A/B heterodimer is known as LEUKOCYTE L1 ANTIGEN COMPLEX. Calgranulin A is found in many cell types including GRANULOCYTES; KERATINOCYTES; and myelomonocytes, and has been shown to act as a chemotactic substance for NEUTROPHILS. Because it is present in acute inflammation but absent in chronic inflammation, it is a useful biological marker for a number of pathological conditions.
A genus of European newts in the Salamandridae family. The two species of this genus are Salamandra salamandra (European "fire" salamander) and Salamandra atra (European alpine salamander).
A nonfluorescent reagent for the detection of primary amines, peptides and proteins. The reaction products are highly fluorescent.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Signal transduction mechanisms whereby calcium mobilization (from outside the cell or from intracellular storage pools) to the cytoplasm is triggered by external stimuli. Calcium signals are often seen to propagate as waves, oscillations, spikes, sparks, or puffs. The calcium acts as an intracellular messenger by activating calcium-responsive proteins.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A nitrocellulose solution in ether and alcohol. Collodion has a wide range of uses in industry including applications in the manufacture of photographic film, in fibers, in lacquers, and in engraving and lithography. In medicine it is used as a drug solvent and a wound sealant.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Unstable isotopes of calcium that decay or disintegrate emitting radiation. Ca atoms with atomic weights 39, 41, 45, 47, 49, and 50 are radioactive calcium isotopes.
A species of parasitic protozoa causing ENTAMOEBIASIS and amebic dysentery (DYSENTERY, AMEBIC). Characteristics include a single nucleus containing a small central karyosome and peripheral chromatin that is finely and regularly beaded.
Voltage-dependent cell membrane glycoproteins selectively permeable to calcium ions. They are categorized as L-, T-, N-, P-, Q-, and R-types based on the activation and inactivation kinetics, ion specificity, and sensitivity to drugs and toxins. The L- and T-types are present throughout the cardiovascular and central nervous systems and the N-, P-, Q-, & R-types are located in neuronal tissue.
A chelating agent relatively more specific for calcium and less toxic than EDETIC ACID.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A network of tubules and sacs in the cytoplasm of SKELETAL MUSCLE FIBERS that assist with muscle contraction and relaxation by releasing and storing calcium ions.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Calcium compounds used as food supplements or in food to supply the body with calcium. Dietary calcium is needed during growth for bone development and for maintenance of skeletal integrity later in life to prevent osteoporosis.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Contractile tissue that produces movement in animals.
Measurement of the intensity and quality of fluorescence.
Proteins that bind to the 3' polyadenylated region of MRNA. When complexed with RNA the proteins serve an array of functions such as stabilizing the 3' end of RNA, promoting poly(A) synthesis and stimulating mRNA translation.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
Stable calcium atoms that have the same atomic number as the element calcium, but differ in atomic weight. Ca-42-44, 46, and 48 are stable calcium isotopes.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
One of the three polypeptide chains that make up the TROPONIN complex of skeletal muscle. It is a calcium-binding protein.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
The physiologically active form of vitamin D. It is formed primarily in the kidney by enzymatic hydroxylation of 25-hydroxycholecalciferol (CALCIFEDIOL). Its production is stimulated by low blood calcium levels and parathyroid hormone. Calcitriol increases intestinal absorption of calcium and phosphorus, and in concert with parathyroid hormone increases bone resorption.
A calcium-binding protein that is 92 AA long, contains 2 EF-hand domains, and is concentrated mainly in GLIAL CELLS. Elevation of S100B levels in brain tissue correlates with a role in neurological disorders.
The sum of the weight of all the atoms in a molecule.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
Transport proteins that carry specific substances in the blood or across cell membranes.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.
A salt used to replenish calcium levels, as an acid-producing diuretic, and as an antidote for magnesium poisoning.
Carbonic acid calcium salt (CaCO3). An odorless, tasteless powder or crystal that occurs in nature. It is used therapeutically as a phosphate buffer in hemodialysis patients and as a calcium supplement.
The rate dynamics in chemical or physical systems.
Established cell cultures that have the potential to propagate indefinitely.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.
The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.
Terbium. An element of the rare earth family of metals. It has the atomic symbol Tb, atomic number 65, and atomic weight 158.92.
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
One of the minor protein components of skeletal muscle. Its function is to serve as the calcium-binding component in the troponin-tropomyosin B-actin-myosin complex by conferring calcium sensitivity to the cross-linked actin and myosin filaments.
Calcium salts of phosphoric acid. These compounds are frequently used as calcium supplements.
A metallic element that has the atomic symbol Mg, atomic number 12, and atomic weight 24.31. It is important for the activity of many enzymes, especially those involved in OXIDATIVE PHOSPHORYLATION.
A family of immunophilin proteins that bind to the immunosuppressive drugs TACROLIMUS (also known as FK506) and SIROLIMUS. EC 5.2.1.-
A poly(A) binding protein that has a variety of functions such as mRNA stabilization and protection of RNA from nuclease activity. Although poly(A) binding protein I is considered a major cytoplasmic RNA-binding protein it is also found in the CELL NUCLEUS and may be involved in transport of mRNP particles.
Proteins prepared by recombinant DNA technology.
Long-lasting voltage-gated CALCIUM CHANNELS found in both excitable and nonexcitable tissue. They are responsible for normal myocardial and vascular smooth muscle contractility. Five subunits (alpha-1, alpha-2, beta, gamma, and delta) make up the L-type channel. The alpha-1 subunit is the binding site for calcium-based antagonists. Dihydropyridine-based calcium antagonists are used as markers for these binding sites.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
A neuronal calcium-sensor protein that was initially found in the NEURONS of the HIPPOCAMPUS. It interacts with NEURONAL APOPTOSIS-INHIBITORY PROTEIN.
A family of soluble proteins that bind insulin-like growth factors and modulate their biological actions at the cellular level. (Int J Gynaecol Obstet 1992;39(1):3-9)
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
The calcium salt of oxalic acid, occurring in the urine as crystals and in certain calculi.
Chemicals that bind to and remove ions from solutions. Many chelating agents function through the formation of COORDINATION COMPLEXES with METALS.
Ytterbium. An element of the rare earth family of metals. It has the atomic symbol Yb, atomic number 70, and atomic weight 173. Ytterbium has been used in lasers and as a portable x-ray source.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Lanthanum. The prototypical element in the rare earth family of metals. It has the atomic symbol La, atomic number 57, and atomic weight 138.91. Lanthanide ion is used in experimental biology as a calcium antagonist; lanthanum oxide improves the optical properties of glass.
The calcium salt of gluconic acid. The compound has a variety of uses, including its use as a calcium replenisher in hypocalcemic states.
A change from planar to elliptic polarization when an initially plane-polarized light wave traverses an optically active medium. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Proteins that bind to RNA molecules. Included here are RIBONUCLEOPROTEINS and other proteins whose function is to bind specifically to RNA.
Positively charged atoms, radicals or groups of atoms with a valence of plus 2, which travel to the cathode or negative pole during electrolysis.
Monomeric subunits of primarily globular ACTIN and found in the cytoplasmic matrix of almost all cells. They are often associated with microtubules and may play a role in cytoskeletal function and/or mediate movement of the cell or the organelles within the cell.
Intracellular proteins that reversibly bind hydrophobic ligands including: saturated and unsaturated FATTY ACIDS; EICOSANOIDS; and RETINOIDS. They are considered a highly conserved and ubiquitously expressed family of proteins that may play a role in the metabolism of LIPIDS.
The level of protein structure in which regular hydrogen-bond interactions within contiguous stretches of polypeptide chain give rise to alpha helices, beta strands (which align to form beta sheets) or other types of coils. This is the first folding level of protein conformation.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.
A process of selective diffusion through a membrane. It is usually used to separate low-molecular-weight solutes which diffuse through the membrane from the colloidal and high-molecular-weight solutes which do not. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A class of organic compounds that contains a naphthalene moiety linked to a sulfonic acid salt or ester.
A poly(A) binding protein that is involved in promoting the extension of the poly A tails of MRNA. The protein requires a minimum of ten ADENOSINE nucleotides in order for binding to mRNA. Once bound it works in conjunction with CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR to stimulate the rate of poly A synthesis by POLY A POLYMERASE. Once poly-A tails reach around 250 nucleotides in length poly(A) binding protein II no longer stimulates POLYADENYLATION. Mutations within a GCG repeat region in the gene for poly(A) binding protein II have been shown to cause the disease MUSCULAR DYSTROPHY, OCULOPHARYNGEAL.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Elements of limited time intervals, contributing to particular results or situations.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.
An autosomal dominant disorder of CONNECTIVE TISSUE with abnormal features in the heart, the eye, and the skeleton. Cardiovascular manifestations include MITRAL VALVE PROLAPSE, dilation of the AORTA, and aortic dissection. Other features include lens displacement (ectopia lentis), disproportioned long limbs and enlarged DURA MATER (dural ectasia). Marfan syndrome is associated with mutations in the gene encoding fibrillin, a major element of extracellular microfibrils of connective tissue.
A group of elements that include SCANDIUM; YTTRIUM; and the LANTHANOID SERIES ELEMENTS. Historically, the rare earth metals got their name from the fact that they were never found in their pure elemental form, but as an oxide. In addition they were very difficult to purify. They are not truly rare and comprise about 25% of the metals in the earth's crust.
Inorganic compounds that contain calcium as an integral part of the molecule.
CALCIUM CHANNELS that are concentrated in neural tissue. Omega toxins inhibit the actions of these channels by altering their voltage dependence.
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
A chelating agent that sequesters a variety of polyvalent cations such as CALCIUM. It is used in pharmaceutical manufacturing and as a food additive.
The muscle tissue of the HEART. It is composed of striated, involuntary muscle cells (MYOCYTES, CARDIAC) connected to form the contractile pump to generate blood flow.
The protein constituents of muscle, the major ones being ACTINS and MYOSINS. More than a dozen accessory proteins exist including TROPONIN; TROPOMYOSIN; and DYSTROPHIN.
One of the six homologous soluble proteins that bind insulin-like growth factors (SOMATOMEDINS) and modulate their mitogenic and metabolic actions at the cellular level.
An atom or group of atoms that have a positive or negative electric charge due to a gain (negative charge) or loss (positive charge) of one or more electrons. Atoms with a positive charge are known as CATIONS; those with a negative charge are ANIONS.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
The relationship between the dose of an administered drug and the response of the organism to the drug.
A family of intracellular calcium-sensing proteins found predominately in NEURONS and PHOTORECEPTOR CELLS. They contain EF HAND MOTIFS and undergo conformational changes upon calcium-binding. Neuronal calcium-sensor proteins interact with other regulatory proteins to mediate physiological responses to a change in intracellular calcium concentration.
Periplasmic proteins that scavenge or sense diverse nutrients. In the bacterial environment they usually couple to transporters or chemotaxis receptors on the inner bacterial membrane.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
Cation-transporting proteins that utilize the energy of ATP hydrolysis for the transport of CALCIUM. They differ from CALCIUM CHANNELS which allow calcium to pass through a membrane without the use of energy.
The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.
A phenothiazine with actions similar to CHLORPROMAZINE. It is used as an antipsychotic and an antiemetic.
A rigorously mathematical analysis of energy relationships (heat, work, temperature, and equilibrium). It describes systems whose states are determined by thermal parameters, such as temperature, in addition to mechanical and electromagnetic parameters. (From Hawley's Condensed Chemical Dictionary, 12th ed)
Commonly observed structural components of proteins formed by simple combinations of adjacent secondary structures. A commonly observed structure may be composed of a CONSERVED SEQUENCE which can be represented by a CONSENSUS SEQUENCE.
A tetrameric calcium release channel in the SARCOPLASMIC RETICULUM membrane of SMOOTH MUSCLE CELLS, acting oppositely to SARCOPLASMIC RETICULUM CALCIUM-TRANSPORTING ATPASES. It is important in skeletal and cardiac excitation-contraction coupling and studied by using RYANODINE. Abnormalities are implicated in CARDIAC ARRHYTHMIAS and MUSCULAR DISEASES.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
A group of enzymes which catalyze the hydrolysis of ATP. The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes may be dependent on Ca(2+), Mg(2+), anions, H+, or DNA.
A photoprotein isolated from the bioluminescent jellyfish Aequorea. It emits visible light by an intramolecular reaction when a trace amount of calcium ion is added. The light-emitting moiety in the bioluminescence reaction is believed to be 2-amino-3-benzyl-5-(p-hydroxyphenyl)pyrazine (AF-350).
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.
Found in various tissues, particularly in four blood-clotting proteins including prothrombin, in kidney protein, in bone protein, and in the protein present in various ectopic calcifications.
Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.
A neuronal calcium sensor protein that is expressed as several isoforms and can interact with ACTIN; TUBULIN; and CLATHRIN.
Agents that increase calcium influx into calcium channels of excitable tissues. This causes vasoconstriction in VASCULAR SMOOTH MUSCLE and/or CARDIAC MUSCLE cells as well as stimulation of insulin release from pancreatic islets. Therefore, tissue-selective calcium agonists have the potential to combat cardiac failure and endocrinological disorders. They have been used primarily in experimental studies in cell and tissue culture.
A 12-KDa tacrolimus binding protein that is found associated with and may modulate the function of calcium release channels. It is a peptidyl-prolyl cis/trans isomerase which is inhibited by both tacrolimus (commonly called FK506) and SIROLIMUS.
A multifunctional protein that is found primarily within membrane-bound organelles. In the ENDOPLASMIC RETICULUM it binds to specific N-linked oligosaccharides found on newly-synthesized proteins and functions as a MOLECULAR CHAPERONE that may play a role in PROTEIN FOLDING or retention and degradation of misfolded proteins. In addition calreticulin is a major storage form for CALCIUM and functions as a calcium-signaling molecule that can regulate intracellular calcium HOMEOSTASIS.
The voltage differences across a membrane. For cellular membranes they are computed by subtracting the voltage measured outside the membrane from the voltage measured inside the membrane. They result from differences of inside versus outside concentration of potassium, sodium, chloride, and other ions across cells' or ORGANELLES membranes. For excitable cells, the resting membrane potentials range between -30 and -100 millivolts. Physical, chemical, or electrical stimuli can make a membrane potential more negative (hyperpolarization), or less negative (depolarization).
The branch of science that deals with the geometric description of crystals and their internal arrangement. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A family of secreted multidomain proteins that were originally identified by their association with the latent form of TRANSFORMING GROWTH FACTORS. They interact with a variety of EXTRACELLULAR MATRIX PROTEINS and may play a role in the regulation of TGB-beta bioavailability.
The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The measurement of the quantity of heat involved in various processes, such as chemical reactions, changes of state, and formations of solutions, or in the determination of the heat capacities of substances. The fundamental unit of measurement is the joule or the calorie (4.184 joules). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A phylum of the kingdom Metazoa. Mollusca have soft, unsegmented bodies with an anterior head, a dorsal visceral mass, and a ventral foot. Most are encased in a protective calcareous shell. It includes the classes GASTROPODA; BIVALVIA; CEPHALOPODA; Aplacophora; Scaphopoda; Polyplacophora; and Monoplacophora.
Family of calcium- and phospholipid-binding proteins which are structurally related and exhibit immunological cross-reactivity. Each member contains four homologous 70-kDa repeats. The annexins are differentially distributed in vertebrate tissues (and lower eukaryotes) and appear to be involved in MEMBRANE FUSION and SIGNAL TRANSDUCTION.
Proteins found in the nucleus of a cell. Do not confuse with NUCLEOPROTEINS which are proteins conjugated with nucleic acids, that are not necessarily present in the nucleus.
The protein components of a number of complexes, such as enzymes (APOENZYMES), ferritin (APOFERRITINS), or lipoproteins (APOLIPOPROTEINS).
A system of cisternae in the CYTOPLASM of many cells. In places the endoplasmic reticulum is continuous with the plasma membrane (CELL MEMBRANE) or outer membrane of the nuclear envelope. If the outer surfaces of the endoplasmic reticulum membranes are coated with ribosomes, the endoplasmic reticulum is said to be rough-surfaced (ENDOPLASMIC RETICULUM, ROUGH); otherwise it is said to be smooth-surfaced (ENDOPLASMIC RETICULUM, SMOOTH). (King & Stansfield, A Dictionary of Genetics, 4th ed)
One of the six homologous soluble proteins that bind insulin-like growth factors (SOMATOMEDINS) and modulate their mitogenic and metabolic actions at the cellular level.
A bile salt formed in the liver by conjugation of deoxycholate with taurine, usually as the sodium salt. It is used as a cholagogue and choleretic, also industrially as a fat emulsifier.
A superorder of marine CRUSTACEA, free swimming in the larval state, but permanently fixed as adults. There are some 800 described species, grouped in several genera, and comprising of two major orders of barnacles: stalked (Pedunculata) and sessile (Sessilia).
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
A protein that has been shown to function as a calcium-regulated transcription factor as well as a substrate for depolarization-activated CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASES. This protein functions to integrate both calcium and cAMP signals.
Processes involved in the formation of TERTIARY PROTEIN STRUCTURE.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
An ionophorous, polyether antibiotic from Streptomyces chartreusensis. It binds and transports CALCIUM and other divalent cations across membranes and uncouples oxidative phosphorylation while inhibiting ATPase of rat liver mitochondria. The substance is used mostly as a biochemical tool to study the role of divalent cations in various biological systems.
A general transcription factor that plays a major role in the activation of eukaryotic genes transcribed by RNA POLYMERASES. It binds specifically to the TATA BOX promoter element, which lies close to the position of transcription initiation in RNA transcribed by RNA POLYMERASE II. Although considered a principal component of TRANSCRIPTION FACTOR TFIID it also takes part in general transcription factor complexes involved in RNA POLYMERASE I and RNA POLYMERASE III transcription.
Organic esters or salts of sulfonic acid derivatives containing an aliphatic hydrocarbon radical.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
A white crystal or crystalline powder used in BUFFERS; FERTILIZERS; and EXPLOSIVES. It can be used to replenish ELECTROLYTES and restore WATER-ELECTROLYTE BALANCE in treating HYPOKALEMIA.
A major protein fraction of milk obtained from the WHEY.
A process leading to shortening and/or development of tension in muscle tissue. Muscle contraction occurs by a sliding filament mechanism whereby actin filaments slide inward among the myosin filaments.
Proteins found in any species of bacterium.
One of the six homologous proteins that specifically bind insulin-like growth factors (SOMATOMEDINS) and modulate their mitogenic and metabolic actions. The function of this protein is not completely defined. However, several studies demonstrate that it inhibits IGF binding to cell surface receptors and thereby inhibits IGF-mediated mitogenic and cell metabolic actions. (Proc Soc Exp Biol Med 1993;204(1):4-29)
The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
A chemical system that functions to control the levels of specific ions in solution. When the level of hydrogen ion in solution is controlled the system is called a pH buffer.
Macromolecular organic compounds that contain carbon, hydrogen, oxygen, nitrogen, and usually, sulfur. These macromolecules (proteins) form an intricate meshwork in which cells are embedded to construct tissues. Variations in the relative types of macromolecules and their organization determine the type of extracellular matrix, each adapted to the functional requirements of the tissue. The two main classes of macromolecules that form the extracellular matrix are: glycosaminoglycans, usually linked to proteins (proteoglycans), and fibrous proteins (e.g., COLLAGEN; ELASTIN; FIBRONECTINS; and LAMININ).
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
The excitable plasma membrane of a muscle cell. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Compounds with three aromatic rings in linear arrangement with an OXYGEN in the center ring.
A theoretical representative nucleotide or amino acid sequence in which each nucleotide or amino acid is the one which occurs most frequently at that site in the different sequences which occur in nature. The phrase also refers to an actual sequence which approximates the theoretical consensus. A known CONSERVED SEQUENCE set is represented by a consensus sequence. Commonly observed supersecondary protein structures (AMINO ACID MOTIFS) are often formed by conserved sequences.
NMR spectroscopy on small- to medium-size biological macromolecules. This is often used for structural investigation of proteins and nucleic acids, and often involves more than one isotope.
Fibrinogens which have a functional defect as the result of one or more amino acid substitutions in the amino acid sequence of normal fibrinogen. Abnormalities of the fibrinogen molecule may impair any of the major steps involved in the conversion of fibrinogen into stabilized fibrin, such as cleavage of the fibrinopeptides by thrombin, polymerization and cross-linking of fibrin. The resulting dysfibrinogenemias can be clinically silent or can be associated with bleeding, thrombosis or defective wound healing.
Proteins which bind with RETINOL. The retinol-binding protein found in plasma has an alpha-1 mobility on electrophoresis and a molecular weight of about 21 kDa. The retinol-protein complex (MW=80-90 kDa) circulates in plasma in the form of a protein-protein complex with prealbumin. The retinol-binding protein found in tissue has a molecular weight of 14 kDa and carries retinol as a non-covalently-bound ligand.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
A white powder prepared from lime that has many medical and industrial uses. It is in many dental formulations, especially for root canal filling.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.
A subtype of striated muscle, attached by TENDONS to the SKELETON. Skeletal muscles are innervated and their movement can be consciously controlled. They are also called voluntary muscles.
A member of the p300-CBP transcription factor family that was initially identified as a binding partner for CAMP RESPONSE ELEMENT-BINDING PROTEIN. Mutations in CREB-binding protein are associated with RUBINSTEIN-TAYBI SYNDROME.
Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.
A non-metal element that has the atomic symbol P, atomic number 15, and atomic weight 31. It is an essential element that takes part in a broad variety of biochemical reactions.
A polypeptide hormone (84 amino acid residues) secreted by the PARATHYROID GLANDS which performs the essential role of maintaining intracellular CALCIUM levels in the body. Parathyroid hormone increases intracellular calcium by promoting the release of CALCIUM from BONE, increases the intestinal absorption of calcium, increases the renal tubular reabsorption of calcium, and increases the renal excretion of phosphates.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Different forms of a protein that may be produced from different GENES, or from the same gene by ALTERNATIVE SPLICING.
A potent vasodilator agent with calcium antagonistic action. It is a useful anti-anginal agent that also lowers blood pressure.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
A calcium salt that is used for a variety of purposes including: building materials, as a desiccant, in dentistry as an impression material, cast, or die, and in medicine for immobilizing casts and as a tablet excipient. It exists in various forms and states of hydration. Plaster of Paris is a mixture of powdered and heat-treated gypsum.
An electrophysiologic technique for studying cells, cell membranes, and occasionally isolated organelles. All patch-clamp methods rely on a very high-resistance seal between a micropipette and a membrane; the seal is usually attained by gentle suction. The four most common variants include on-cell patch, inside-out patch, outside-out patch, and whole-cell clamp. Patch-clamp methods are commonly used to voltage clamp, that is control the voltage across the membrane and measure current flow, but current-clamp methods, in which the current is controlled and the voltage is measured, are also used.
An essential amino acid that is necessary for normal growth in infants and for NITROGEN balance in adults. It is a precursor of INDOLE ALKALOIDS in plants. It is a precursor of SEROTONIN (hence its use as an antidepressant and sleep aid). It can be a precursor to NIACIN, albeit inefficiently, in mammals.
A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.
An alpha-globulin found in the plasma of man and other vertebrates. It is apparently synthesized in the liver and carries vitamin D and its metabolites through the circulation and mediates the response of tissue. It is also known as group-specific component (Gc). Gc subtypes are used to determine specific phenotypes and gene frequencies. These data are employed in the classification of population groups, paternity investigations, and in forensic medicine.
A class of proteins that were originally identified by their ability to bind the DNA sequence CCAAT. The typical CCAAT-enhancer binding protein forms dimers and consists of an activation domain, a DNA-binding basic region, and a leucine-rich dimerization domain (LEUCINE ZIPPERS). CCAAT-BINDING FACTOR is structurally distinct type of CCAAT-enhancer binding protein consisting of a trimer of three different subunits.
The concentration of osmotically active particles in solution expressed in terms of osmoles of solute per liter of solution. Osmolality is expressed in terms of osmoles of solute per kilogram of solvent.
Artifactual vesicles formed from the endoplasmic reticulum when cells are disrupted. They are isolated by differential centrifugation and are composed of three structural features: rough vesicles, smooth vesicles, and ribosomes. Numerous enzyme activities are associated with the microsomal fraction. (Glick, Glossary of Biochemistry and Molecular Biology, 1990; from Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
The deductive study of shape, quantity, and dependence. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
A fluorescent calcium chelating agent which is used to study intracellular calcium in tissues.
A member of the alkali group of metals. It has the atomic symbol Na, atomic number 11, and atomic weight 23.
A RNA-binding protein that binds to polypyriminidine rich regions in the INTRONS of messenger RNAs. Polypyrimidine tract-binding protein may be involved in regulating the ALTERNATIVE SPLICING of mRNAs since its presence on an intronic RNA region that is upstream of an EXON inhibits the splicing of the exon into the final mRNA product.
Measurement of the polarization of fluorescent light from solutions or microscopic specimens. It is used to provide information concerning molecular size, shape, and conformation, molecular anisotropy, electronic energy transfer, molecular interaction, including dye and coenzyme binding, and the antigen-antibody reaction.
A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed)
A diverse superfamily of proteins that function as translocating proteins. They share the common characteristics of being able to bind ACTINS and hydrolyze MgATP. Myosins generally consist of heavy chains which are involved in locomotion, and light chains which are involved in regulation. Within the structure of myosin heavy chain are three domains: the head, the neck and the tail. The head region of the heavy chain contains the actin binding domain and MgATPase domain which provides energy for locomotion. The neck region is involved in binding the light-chains. The tail region provides the anchoring point that maintains the position of the heavy chain. The superfamily of myosins is organized into structural classes based upon the type and arrangement of the subunits they contain.
The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)
A sesquiterpene lactone found in roots of THAPSIA. It inhibits CA(2+)-TRANSPORTING ATPASE mediated uptake of CALCIUM into SARCOPLASMIC RETICULUM.
One of the six homologous soluble proteins that bind insulin-like growth factors (SOMATOMEDINS) and modulate their mitogenic and metabolic actions at the cellular level.

Endocytosis: EH domains lend a hand. (1/7886)

A number of proteins that have been implicated in endocytosis feature a conserved protein-interaction module known as an EH domain. The three-dimensional structure of an EH domain has recently been solved, and is likely to presage significant advances in understanding molecular mechanisms of endocytosis.  (+info)

Binding of the G domains of laminin alpha1 and alpha2 chains and perlecan to heparin, sulfatides, alpha-dystroglycan and several extracellular matrix proteins. (2/7886)

The C-terminal G domain of the mouse laminin alpha2 chain consists of five lamin-type G domain (LG) modules (alpha2LG1 to alpha2LG5) and was obtained as several recombinant fragments, corresponding to either individual modules or the tandem arrays alpha2LG1-3 and alpha2LG4-5. These fragments were compared with similar modules from the laminin alpha1 chain and from the C-terminal region of perlecan (PGV) in several binding studies. Major heparin-binding sites were located on the two tandem fragments and the individual alpha2LG1, alpha2LG3 and alpha2LG5 modules. The binding epitope on alpha2LG5 could be localized to a cluster of lysines by site-directed mutagenesis. In the alpha1 chain, however, strong heparin binding was found on alpha1LG4 and not on alpha1LG5. Binding to sulfatides correlated to heparin binding in most but not all cases. Fragments alpha2LG1-3 and alpha2LG4-5 also bound to fibulin-1, fibulin-2 and nidogen-2 with Kd = 13-150 nM. Both tandem fragments, but not the individual modules, bound strongly to alpha-dystroglycan and this interaction was abolished by EDTA but not by high concentrations of heparin and NaCl. The binding of perlecan fragment PGV to alpha-dystroglycan was even stronger and was also not sensitive to heparin. This demonstrated similar binding repertoires for the LG modules of three basement membrane proteins involved in cell-matrix interactions and supramolecular assembly.  (+info)

Identification of a novel family of targets of PYK2 related to Drosophila retinal degeneration B (rdgB) protein. (3/7886)

The protein tyrosine kinase PYK2 has been implicated in signaling pathways activated by G-protein-coupled receptors, intracellular calcium, and stress signals. Here we describe the molecular cloning and characterization of a novel family of PYK2-binding proteins designated Nirs (PYK2 N-terminal domain-interacting receptors). The three Nir proteins (Nir1, Nir2, and Nir3) bind to the amino-terminal domain of PYK2 via a conserved sequence motif located in the carboxy terminus. The primary structures of Nirs reveal six putative transmembrane domains, a region homologous to phosphatidylinositol (PI) transfer protein, and an acidic domain. The Nir proteins are the human homologues of the Drosophila retinal degeneration B protein (rdgB), a protein implicated in the visual transduction pathway in flies. We demonstrate that Nirs are calcium-binding proteins that exhibit PI transfer activity in vivo. Activation of PYK2 by agents that elevate intracellular calcium or by phorbol ester induce tyrosine phosphorylation of Nirs. Moreover, PYK2 and Nirs exhibit similar expression patterns in several regions of the brain and retina. In addition, PYK2-Nir complexes are detected in lysates prepared from cultured cells or from brain tissues. Finally, the Nir1-encoding gene is located at human chromosome 17p13.1, in proximity to a locus responsible for several human retinal diseases. We propose that the Nir and rdgB proteins represent a new family of evolutionarily conserved PYK2-binding proteins that play a role in the control of calcium and phosphoinositide metabolism downstream of G-protein-coupled receptors.  (+info)

Phospholamban is present in endothelial cells and modulates endothelium-dependent relaxation. Evidence from phospholamban gene-ablated mice. (4/7886)

Vascular endothelial cells regulate vascular smooth muscle tone through Ca2+-dependent production and release of vasoactive molecules. Phospholamban (PLB) is a 24- to 27-kDa phosphoprotein that modulates activity of the sarco(endo)plasmic reticulum Ca2+ ATPase (SERCA). Expression of PLB is reportedly limited to cardiac, slow-twitch skeletal and smooth muscle in which PLB is an important regulator of [Ca2+]i and contractility in these muscles. In the present study, we report the existence of PLB in the vascular endothelium, a nonmuscle tissue, and provide functional data on PLB regulation of vascular contractility through its actions in the endothelium. Endothelium-dependent relaxation to acetylcholine was attenuated in aorta of PLB-deficient (PLB-KO) mice compared with wild-type (WT) controls. This effect was not due to actions of nitric oxide on the smooth muscle, because sodium nitroprusside-mediated relaxation in either denuded or endothelium-intact aortas was unaffected by PLB ablation. Relative to denuded vessels, relaxation to forskolin was enhanced in WT endothelium-intact aortas. The endothelium-dependent component of this relaxation was attenuated in PLB-KO aortas. To investigate whether these changes were due to PLB, WT mouse aorta endothelial cells were isolated. Both reverse transcriptase-polymerase chain reaction and Western blot analyses revealed the presence of PLB in endothelial cells, which were shown to be >98% pure by diI-acetylated LDL uptake and nuclear counterstaining. These data indicate that PLB is present and modulates vascular function as a result of its actions in endothelial cells. The presence of PLB in endothelial cells opens new fields for investigation of Ca2+ regulatory pathways in nonmuscle cells and for modulation of endothelial-vascular interactions.  (+info)

A novel interaction mechanism accounting for different acylphosphatase effects on cardiac and fast twitch skeletal muscle sarcoplasmic reticulum calcium pumps. (5/7886)

In cardiac and skeletal muscle Ca2+ translocation from cytoplasm into sarcoplasmic reticulum (SR) is accomplished by different Ca2+-ATPases whose functioning involves the formation and decomposition of an acylphosphorylated phosphoenzyme intermediate (EP). In this study we found that acylphosphatase, an enzyme well represented in muscular tissues and which actively hydrolyzes EP, had different effects on heart (SERCA2a) and fast twitch skeletal muscle SR Ca2+-ATPase (SERCA1). With physiological acylphosphatase concentrations SERCA2a exhibited a parallel increase in the rates of both ATP hydrolysis and Ca2+ transport; in contrast, SERCA1 appeared to be uncoupled since the stimulation of ATP hydrolysis matched an inhibition of Ca2+ pump. These different effects probably depend on phospholamban, which is associated with SERCA2a but not SERCA1. Consistent with this view, the present study suggests that acylphosphatase-induced stimulation of SERCA2a, in addition to an enhanced EP hydrolysis, may be due to a displacement of phospholamban, thus to a removal of its inhibitory effect.  (+info)

Oligosaccharide modification in the early secretory pathway directs the selection of a misfolded glycoprotein for degradation by the proteasome. (6/7886)

The role of conformation-based quality control in the early secretory pathway is to eliminate misfolded polypeptides and unassembled multimeric protein complexes from the endoplasmic reticulum, ensuring the deployment of only functional molecules to distal sites. The intracellular fate of terminally misfolded human alpha1-antitrypsin was examined in hepatoma cells to identify the functional role of asparagine-linked oligosaccharide modification in the selection of glycoproteins for degradation by the cytosolic proteasome. Proteasomal degradation required physical interaction with the molecular chaperone calnexin. Altered sedimentation of intracellular complexes following treatment with the specific proteasome inhibitor lactacystin, and in combination with mannosidase inhibition, revealed that the removal of mannose from attached oligosaccharides abrogates the release of misfolded alpha1-antitrypsin from calnexin prior to proteasomal degradation. Intracellular turnover was arrested with kifunensine, implicating the participation of endoplasmic reticulum mannosidase I in the disposal process. Accelerated degradation occurred in a mannosidase-independent manner and was arrested by lactacystin, in response to the posttranslational inhibition of glucosidase II, demonstrating that the attenuated removal of glucose from attached oligosaccharides functions as the underlying rate-limiting step in the proteasome-mediated pathway. A model is proposed in which the removal of mannose from multiple attached oligosaccharides directs calnexin in the selection of misfolded alpha1-antitrypsin for degradation by the proteasome.  (+info)

Complete exon-intron organization of the mouse fibulin-1 gene and its comparison with the human fibulin-1 gene. (7/7886)

Fibulin-1 is a 90 kDa calcium-binding protein present in the extracellular matrix and in the blood. Two major variants, C and D, differ in their C-termini as well as the ability to bind the basement membrane protein nidogen. Here we characterized genomic clones encoding the mouse fibulin-1 gene, which contains 18 exons spanning at least 75 kb of DNA. The two variants are generated by alternative splicing of exons in the 3' end. By searching the database we identified most of the exons encoding the human fibulin-1 gene and showed that its exon-intron organization is similar to that of the mouse gene.  (+info)

Function of WW domains as phosphoserine- or phosphothreonine-binding modules. (8/7886)

Protein-interacting modules help determine the specificity of signal transduction events, and protein phosphorylation can modulate the assembly of such modules into specific signaling complexes. Although phosphotyrosine-binding modules have been well-characterized, phosphoserine- or phosphothreonine-binding modules have not been described. WW domains are small protein modules found in various proteins that participate in cell signaling or regulation. WW domains of the essential mitotic prolyl isomerase Pin1 and the ubiquitin ligase Nedd4 bound to phosphoproteins, including physiological substrates of enzymes, in a phosphorylation-dependent manner. The Pin1 WW domain functioned as a phosphoserine- or phosphothreonine-binding module, with properties similar to those of SRC homology 2 domains. Phosphoserine- or phosphothreonine-binding activity was required for Pin1 to interact with its substrates in vitro and to perform its essential function in vivo.  (+info)

Allograft inflammatory factor-1 (AIF-1) is an evolutionary conserved protein important to inflammatory responses throughout the body including that of microglia in the central nervous system (CNS). In addition to critical intracellular roles in the activation of microglia and macrophages, AIF-1 can be secreted by these cells in response to inflammatory signals as well as soluble signals released by dying neurons. In response to the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine, we found increased levels of AIF-1 expression in cells clustered in the substantia nigra pars compacta (SNpc), the site of dopaminergic cell death. The number of these AIF-1 bright cells continued to increase even after neuronal cell death was complete. This increased expression of AIF-1 was restricted to resident microglia; flow cytometric analysis showed that infiltrating CD45hi leukocytes did not express high levels of AIF-1. Analysis of microglia ex vivo demonstrated the secretion of AIF-1 by these cells, ...
TY - JOUR. T1 - The senescence marker protein (SMP-2) of the rat liver. T2 - purification, immunochemical characterization and age-dependent regulation. AU - Chatterjee, Bandana. AU - Mancini, Michael A.. AU - Roy, Arun K.. N1 - Funding Information: The work was supportedb y NIH grant AG-03527. A.K.R. is the recipient of a MERIT Award from NIDDK. We thank MasarratA li, Richard Lorch and C.V.R. Murty for their contributiontso this work.. PY - 1990/5/16. Y1 - 1990/5/16. N2 - In vitro translation of total rat hepatic mRNAs has identified a 31 kilodalton senescence marker protein (SMP-2) which is present in higher amounts in prepubertal and senescent males than in the post-pubertal adult male (more than 10-fold). SMP-2 is an androgen-repressible protein. The negative regulation of the SMP-2 gene activity by androgen accounts for its increased expression during the androgen insensitive states of the prepubertal and senescent livers, and its constitutive expresion in the female liver. A combination of ...
TY - JOUR. T1 - Melatonin ameliorates brain oxidative stress and upregulates senescence marker protein-30 and osteopontin in a rat model of vascular dementia. AU - Bin-Jaliah, I.. AU - Sakr, H. F.. PY - 2018/3. Y1 - 2018/3. N2 - The aim of this study was to investigate the effect of melatonin on oxidative stress and senescence marker protein-30 (SMP30) as well as osteopontin (OPN) expression in the hippocampus of rats subjected to vascular dementia (VD). A total of 72 male rats were divided into six groups (n = 12 each) as follows: (i) untreated control (CON), (ii) sham-operated group, (iii) sham-operated + melatonin, (iv) rats exposed to VD induced by permanent bilateral occlusion of the common carotid arteries (BCCAO) leading to chronic cerebral hypoperfusion, (v) rats exposed to VD + melatonin, and (vi) rats exposed to VD + donepezil (DON). At the end of experiment, the hippocampal levels of acetylcholine (ACh), norepinephrine (NE), and dopamine (Dop) were measured. Expression of OPN was ...
Fingerprint Dive into the research topics of Regulation of Fas ligand expression and cell death by apoptosis-linked gene 4. Together they form a unique fingerprint. ...
Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na(+)/H(+) exchange activity. Promotes the maturation, transport, cell surface stability and exchange activity of SLC9A1/NHE1 at the plasma membrane. Promotes the induction of hematopoietic stem cell differentiation toward megakaryocytic lineage. Essential for the coupling of ERK cascade activation with the expression of ETS family genes in megakaryocytic differentiation. Also involved in granulocytic differentiation in a ERK-dependent manner. Inhibits the phosphatase activity of calcineurin.
Early myocardial reperfusion is an effective therapy but ischemia/reperfusion (I/R) causes lethal myocardial injury. The aging heart was reported to show greater cardiac damage after I/R injury than that observed in young hearts. Senescence marker protein 30 (SMP30), whose expression decreases with age, plays a role in reducing oxidative stress and apoptosis. However, the impact of SMP30 on myocardial I/R injury remains to be determined. In this study, the left anterior descending coronary artery was occluded for 30 min, followed by reperfusion in wild-type (WT) and SMP30 knockout (KO) mice. After I/R, cardiomyocyte apoptosis and the ratio of infarct area/area at risk were higher, left ventricular fractional shortening was lower, and reactive oxygen species (ROS) generation was enhanced in SMP30 KO mice. Moreover, the previously increased phosphorylation of GSK-3β and Akt was lower in SMP30 KO mice than in WT mice. In cardiomyocytes, silencing of SMP30 expression attenuated Akt and GSK-3β
Buy our Recombinant Human Phospholamban protein. Ab114227 is a protein fragment produced in Wheat germ and has been validated in WB, ELISA, SDS-PAGE. Abcam…
Cardiovascular disease (CVD) is the leading cause of death in developed societies. The main contributor to CVD is atherosclerosis, a complex disease in which accumulation of lipoprotein deposits in arterial walls results in a chronic inflammatory response that leads to plaque formation. The macrophage, in particular, has been shown to play a fundamental role in the inflammatory response that promotes atherosclerosis. The Allograft Inflammatory Factor (Aif-1), also known as Ionized Binding Adapter-1 (Iba-1), is a 17 kDa cytoplasmic EF-hand protein expressed in vascular lesions with atherogenic characteristics. Although substantial evidence links Aif-1 to the inflammatory response, its role in macrophage biological functions remains largely unexplored. Definitive studies of Aif-1 loss-of-function have not been reported; here we present an analysis of aif-1 inactivation by gene targeting in mice on blood cell populations, macrophage function, and the pathogenesis of atherosclerosis in a ...
Gentaur molecular products has all kinds of products like :search , QED \ Human Calpastatin mono \ 56319 for more molecular products just contact us
Among the EF-Hand calcium-binding proteins the subgroup of S100 proteins constitute a large family with numerous and diverse functions in calcium-mediated signaling. The evolutionary origin of this family is still uncertain and most studies have examined mammalian family members. We have performed an extensive search in several teleost genomes to establish the s100 gene family in fish. We report that the teleost S100 repertoire comprises fourteen different subfamilies which show remarkable similarity across six divergent teleost species. Individual species feature distinctive subsets of thirteen to fourteen genes that result from local gene duplications and gene losses. Eight of the fourteen S100 subfamilies are unique for teleosts, while six are shared with mammalian species and three of those even with cartilaginous fish. Several S100 family members are found in jawless fish already, but none of them are clear orthologs of cartilaginous or bony fish s100 genes. All teleost s100 genes show the expected
Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation (By similarity).
Alix [ALG-2 (apoptosis-linked gene 2)-interacting protein X] is a ubiquitinous adaptor protein first described for its capacity to bind to the calcium-binding protein, ALG-2. Alix regulates neuronal death in ways involving interactions with ALG-2 and with proteins of the ESCRT (endosomal sorting complex required for transport). Even though all Alix interactors characterized to date are involved in endosomal trafficking, the genuine function of the protein in this process remains unclear. We have demonstrated recently that Alix and ALG-2 form in the presence of calcium, a complex with apical caspases and with the endocytosed death receptor TNFR1 (tumour necrosis factor α receptor 1), thus suggesting a molecular coupling between endosomes and the cell death machinery.. ...
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casSAR Dugability of Q9DB16 | Cab39l | Calcium-binding protein 39-like - Also known as CB39L_MOUSE, Cab39l. Component of a complex that binds and activates STK11/LKB1. In the complex, required to stabilize the interaction between CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta) and STK11/LKB1 (By similarity). Component of a trimeric complex composed of STK11/LKB1, STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta): the complex tethers STK11/LKB1 in the cytoplasm and stimulates its catalytic activity.
Phospholamban Phospholamban pentamer Identifiers Symbol Phospholamban Pfam PF04272 InterPro IPR005984 SCOP 1fjk TCDB 8.A.11 OPM family 70 OPM protein 1zll
Opens the Highlight Feature Bar and highlights feature annotations from the FEATURES table of the record. The Highlight Feature Bar can be used to navigate to and highlight other features and provides links to display the highlighted region separately. Links in the FEATURES table will also highlight the corresponding region of the sequence. More... ...
One of the analyzed signal transduction systems mediates the specific transmission of calcium signals. One type of calcium sensors are the AtCBL (Calcineurin B-like) protein family comprising of ten members in Arabidopsis ...
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein has a Ca2+ affinity 20- to 100-fold higher than the other S100 proteins studied under identical conditions. This protein also binds Zn2+ and Cu2+, and Cu2+ strongly which impairs the binding of Ca2+. This protein is expressed in very restricted regions of the adult brain. [provided by RefSeq, Jul 2008 ...
The Recombinant Human NUCB2 (Nesfatin) produced in E.coli has a molecular mass of 9.7kDa containing 82 amino acid residues of the human NUCB2.
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Sigma-Aldrich offers abstracts and full-text articles by [Leyuan Bao, Adam F Odell, Sam L Stephen, Stephen B Wheatcroft, John H Walker, Sreenivasan Ponnambalam].
Cabyr (untagged) - Mouse calcium-binding tyrosine-(Y)-phosphorylation regulated (fibrousheathin 2) (Cabyr), transcript variant 2, (10ug), 10 µg.
oncomodulin: cytoplasmic calcium-binding protein found only in tumor cells; MW 11,500; not a fragment of calmodulin; stimulates liver cell DNA synthesis
Clotting factors VII, IX, and X and prothrombin all require carboxylation of glutamate residues for functional activity. Carboxylation provides calcium-binding sites and thus allows calcium-dependent interaction of these clotting factors with a phospholipid surface involved in the generation of thrombin ...
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. [provided by RefSeq, Jul 2008 ...
Providing premium quality antibodies to cardiovascular phosphorylated targets. Fully validated by western blotting and immunofluorescence microscopy by PhD scientists. We supply antibodies to Phospholamban, SERCA, RYR2 and their phosphorylated forms.
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Fingerprint Dive into the research topics of Bone vitamin D-dependent calcium-binding protein is localized in chondrocytes of growth-plate cartilage. Together they form a unique fingerprint. ...
The 67 kDa calcimedin is a Ca2+-binding protein isolated from several muscle tissues. A recent report [Morse & Moore (1988) Biochem. J. 251, 171-174] indicated that the 67 kDa calcimedin is distinct from 67 kDa calelectrin, which is purified from various non-muscle cells. In the present study we have purified the 67 kDa protein from bovine aorta (i.e. 67 kDa calcimedin) and liver (i.e. 67 kDa calelectrin) and compared them by immunological and biochemical criteria. The aorta calcimedin is identical with the liver calelectrin by the following criteria. (1) The calcimedin co-electrophoresed with the calelectrin on SDS/5-15%-(w/v)-linear-gradient polyacrylamide gels. (2) The two proteins selectively cross-reacted with a chicken gizzard calcimedin antibody. (3) An antibody raised against the bovine aorta calcimedin also recognized the bovine liver calelectrin. (4) One-dimensional peptide maps of the two proteins revealed no significant difference. (5) The calcimedin appeared to have an amino acid ...
Vitamin D-dependent calcium binding proteins were discovered in the cytosolic fractions of chicken intestine, and later in mammalian intestine and kidney, by workers including Robert Wasserman of Cornell University. They bound calcium in the micromolar range and were greatly reduced in vitamin D-deficient animals. Expression could be induced by treating these animals with vitamin D metabolites such as calcitriol. They were found to exist in two distinct sizes with a molecular weight of approximately 9 kDa and 28 kDa. They were renamed calbindin. Calbindin-D9k (S100G) is found in mammalian intestine and calbindin-D28k is in avian intestine and in mammalian kidney and other tissues. Calcium-binding protein Wasserman, RH; Taylor, AN (1966). Vitamin D3-induced calcium-binding protein in chick intestinal mucosa. Science. 152 (3723): 791-3. doi:10.1126/science.152.3723.791. PMID 17797460. Wasserman, RH; Corradino, RA; Taylor, AN (1969). Binding proteins from animals with possible transport ...
Looking for online definition of S100 calcium-binding protein A13 in the Medical Dictionary? S100 calcium-binding protein A13 explanation free. What is S100 calcium-binding protein A13? Meaning of S100 calcium-binding protein A13 medical term. What does S100 calcium-binding protein A13 mean?
1. The rapid stimulation of intestinal Ca2+transport observed in vitamin D-deficient chicks after receiving 1,25-dihydroxycholecalciferol has necessitated a re-evaluation of the correlation hitherto observed between this stimulation and the induction of calcium-binding protein synthesis. By 1h after a dose of 125ng of 1,25-dihydroxycholecalciferol, Ca2+transport is increased. This is at least 2h before calcium-binding protein can be detected immunologically and 1h before synthesis of the protein begins on polyribosomes, and thus the hormone stimulates Ca2+transport before calcium-binding-protein biosynthesis is induced. 2. The maximum increase in Ca2+transport observed after this dose of 1,25-dihydroxycholecalciferol (attained by 8h) is similar to that observed after 1.25-25μg of cholecalciferol, but the stimulation is only short-lived, in contrast with the effect observed after the vitamin. At later times after the hormone, however, when Ca2+transport has declined to its basal rate, the ...
TY - JOUR. T1 - Smooth muscle calponin. T2 - An unconventional CArG-dependent gene that antagonizes neointimal formation. AU - Long, Xiaochun. AU - Slivano, Orazio J.. AU - Cowan, Sarah L.. AU - Georger, Mary A.. AU - Lee, Ting Hein. AU - Miano, Joseph M.. PY - 2011/10/1. Y1 - 2011/10/1. N2 - OBJECTIVE-: Smooth muscle calponin (CNN1) contains multiple conserved intronic CArG elements that bind serum response factor and display enhancer activity in vitro. The objectives here were to evaluate these CArG elements for activity in transgenic mice and determine the effect of human CNN1 on injury-induced vascular remodeling. METHODS AND RESULTS-: Mice carrying a lacZ reporter under control of intronic CArG elements in the human CNN1 gene failed to show smooth muscle cell (SMC)-restricted activity. However, deletion of the orthologous sequences in mice abolished endogenous Cnn1 promoter activity, suggesting their necessity for in vivo Cnn1 expression. Mice carrying a 38-kb bacterial artificial ...
Ca2+-dependent activator protein for secretion (CAPS) regulates exocytosis of catecholamine- or neuropeptide-containing dense-core vesicles (DCVs) at secretion sites such as nerve terminals. studies focused on the role of CAPS protein in exocytosis at secretion sites such as nerve terminals or the cell periphery a large fraction of CAPS protein in many neuronal cell types is actually localized in the soma rather than at these secretion sites (2 13 14 Therefore the HA-1077 role of HA-1077 somal CAPS proteins which constitute the largest amount of CAPS proteins in the cell also needs to be elucidated. In this study we have investigated the role of somal CAPS1 proteins in the DCV secretory pathway including biogenesis-trafficking-secretion events. We showed an conversation between CAPS1 and the class II ADP-ribosylation factor (ARF) small GTPase around the Golgi membrane depending on the ARF-GDP mode. The ARF family consists of three classes and a total of six users: class I consisting of ARF1 to ...
Head and neck squamous cell carcinoma express high levels of the EF-hand calcium-binding protein S100A2 in contrast to other tumorigenic tissues and cell lines where the expression of this protein is reduced. Subtractive hybridization of tumorigenic versus normal tumor-derived mammary epithelial cells has previously identified the S100A2 protein as potential tumor suppressor. The biological function of S100A2 in carcinogenesis, however, has not been elucidated to date. Here, we report for the first time that during recovery from hydroxyurea treatment, the S100A2 protein translocated from the cytoplasm to the nucleus and co-localized with the tumor suppressor p53 in two different oral carcinoma cells (FADU and SCC-25). Co-immunoprecipitation experiments and electrophoretic mobility shift assay showed that the interaction between S100A2 and p53 is Ca(2+)-dependent. Preliminary characterization of this interaction indicated that the region in p53 involved with binding to S100A2 is located at the C ...
Comparative Studies on Metabolic Rate and Calpain/Calpastatin Activity between Hanwoo and Holstein Beef - Hanwoo Beef;Holstein Beef;Temperature Conditioning;Metabolic Rate;Calpain;Calpastatin;
Calpastatin (CAST) is a calpain inhibitor, a calcium-dependent cysteine protease that is widely distributed in higher order animals. There are different types of calpastatins; examples include the 68 kDa erythrocyte-derived calpastatin and the 107 kDa myocyte-derived calpastatin (as evidenced by SDS-PAGE). Through its inhibition of calpain, calpastatin is believed to play important roles in the regulation of cell proliferation, differentiation and aging. CAST is also involved in the proteolysis of amyloid precursor protein (APP). ...
Calpastatin (CAST) is a calpain inhibitor, a calcium-dependent cysteine protease that is widely distributed in higher order animals. There are different types of calpastatins; examples include the 68 kDa erythrocyte-derived calpastatin and the 107 kDa myocyte-derived calpastatin (as evidenced by SDS-PAGE). Through its inhibition of calpain, calpastatin is believed to play important roles in the regulation of cell proliferation, differentiation and aging. CAST is also involved in the proteolysis of amyloid precursor protein (APP). ...
Expression of ECM proteins fibulin-1 and -2 in acute and chronic liver disease and in cultured rat liver cells. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
S100 Calcium Binding Protein B Human Recombinant, S100 Calcium Binding Protein B, S-100 Protein Subunit Beta, S100 Calcium Binding Protein, Beta (Neural), S100 Calcium-Binding Protein, Beta (Neural), S-100 Calcium-Binding Protein, Beta Chain, S100 Calcium-Binding Protein B, S-100 Protein Beta Chain, S100beta, S100-B, S100, NEF.
Neocortical neurones can be classified according to several independent criteria: morphological, physiological, and molecular expression (neuropeptides (NPs) and/or calcium-binding proteins (CaBPs)). While it has been suggested that particular NPs and CaBPs characterize certain anatomical subtypes of neurones, there is also considerable overlap in their expression, and little is known about simultaneous expression of multiple NPs and CaBPs in morphologically characterized neocortical neurones. Here we determined the gene expression profiles of calbindin (CB), parvalbumin (PV), calretinin (CR), neuropeptide Y (NPY), vasoactive intestinal peptide (VIP), somatostatin (SOM) and cholecystokinin (CCK) in 268 morphologically identified neurones located in layers 2-6 in the juvenile rat somatosensory neocortex. We used patch-clamp electrodes to label neurones with biocytin and harvest the cytoplasm to perform single-cell RT-multiplex PCR. Quality threshold clustering, an unsupervised algorithm that clustered
casSAR Dugability of Q9D581 | Efcab10 | EF-hand calcium-binding domain-containing protein 10 - Also known as EFC10_MOUSE, Efcab10.
Phospholamban (PLN) regulates myocyte calcium cycling by inhibiting the Ca2+ATPase SERCA2a. Protein kinase A (PKA) mediated phosphorylation attenuates PLN activity leading to enhanced calcium uptake rates and accelerated cardiac relaxation. In vivo, PLN is present in monomeric and pentameric form. It is believed that PKA primarily targets the PLN monomer. However, we found that a R9C mutant of PLN dominantly inhibits PLN phosphorylation only within the pentamer suggesting a significant role of the pentamer in determining phosphorylation and, thus, PLN activity.. To investigate the role of the pentamer in PLN phosphorylation and function, the sensitivity, kinetics and stoichiometry of phosphorylation were analyzed in monomeric and pentameric PLN mutants expressed in a human cell line (HEK293AD). We found an independent increase of phosphorylation for monomer and pentamer upon forskolin stimulation, both in a concentration and time-dependent manner. Intriguingly, phosphorylation signals of PLN ...
Calcium-Binding Proteins: Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS.
Dive into the research topics of Structural and functional characterization of the extracellular calcium-binding protein BM-40/secreted protein, acidic, rich in cysteine/osteonectin from the nematode Caenorhabditis elegans. Together they form a unique fingerprint. ...
The secretory pathway Ca\(^{2+}\)-ATPase (SPCA) provides the Golgi apparatus with a luminal Ca\(^{2+}\) store, which is used to modulate the activity of Ca\(^{2+}\)-dependent enzymes involved in controlling the secretory pathway and post-translational modification of proteins. This Ca\(^{2+}\) store controlled by SPCA is also believed to be agonist-releasable. Regucalcin (RGN), (also known as senescence marker protein 30 (SMP30)) is believed to be a Ca\(^{2+}\)-binding protein expressed in an age-dependent manner, whereby its protein levels decrease in a number of organs as aging progresses. It has been suggested to be able to affect the activities of the sarco/endo-plasmic reticulum Ca\(^{2+}\)-ATPase (SERCA), as well as other Ca\(^{2+}\)-dependent enzymes. On the other hand, RGNs ability to bind Ca\(^{2+}\) has been argued against and this protein has been shown to modulate the activities of enzymes not involved in Ca\(^{2+}\) homeostasis, as well as have intrinsic enzymatic activity in ...
Background Nucleobindin 2 (NUCB2) abnormal manifestation continues to be reported in gastric cancers and breast cancer tumor. might play an optimistic function in PCa advancement and may serve as an unbiased predictor of BCR-free success. Background Prostate cancers (PCa) is among the most regularly diagnosed malignancies and a common reason behind cancer tumor mortality in guys in the Traditional western hemisphere [1], which includes SB-705498 become a main public health problem. In SB-705498 China, the incidence of PCa continues to be increasing in the newest years continually. Although weve made considerable developments in medical diagnosis and adjuvant therapy of PCa, the entire survival rate of PCa patients markedly is not improved. The mechanism of its carcinogenesis, like additional cancers, is still not fully recognized. It is a clinically heterogeneous, multifocal disease. Carcinogenesis and mechanisms influencing progression and prognosis of PCa are a multi-step process, including ...
Fingerprint Dive into the research topics of Temporal response of rabbits to beta-adrenergic agonist feeding: tissue weight, calpains and calpastatin activities, and nucleic acid and protein concentrations.. Together they form a unique fingerprint. ...
Fimbrins are EF-hand calcium-binding proteins that actively participate in binding to and bundling of actin. In actin filaments, one molecule of fimbrin might
Calpain-like mRNAs have been identified in other organisms including bacteria, but the molecules encoded by these mRNAs have not been isolated, so little is known about their properties. How calpain activity is regulated in these organisms cells is still unclear In metazoans, the activity of calpain is controlled by a single proteinase inhibitor, calpastatin (IPR001259). The calpastatin gene can produce eight or more calpastatin polypeptides ranging from 17 to 85 kDa by use of different promoters and alternative splicing events. The physiological significance of these different calpastatins is unclear, although all bind to three different places on the calpain molecule; binding to at least two of the sites is Ca2+ dependent. The calpains ostensibly participate in a variety of cellular processes including remodelling of cytoskeletal/membrane attachments, different signal transduction pathways, and apoptosis. Deregulated calpain activity following loss of Ca2+ homeostasis results in tissue damage ...
Buy our Recombinant Human S100 Calcium Binding Protein A13. Ab125667 is a full length protein produced in Escherichia coli and has been validated in SDS-PAGE…
Many calcium-binding proteins belong to the same evolutionary family and share a type of calcium-binding domain known as the EF-hand [1,2,3,4,5]. This type of domain consists of a twelve residue loop flanked on both side by a twelve residue α-helical domain (see ,PDB:1CLL,). In an EF-hand loop the calcium ion is coordinated in a pentagonal bipyramidal configuration. The six residues involved in the binding are in positions 1, 3, 5, 7, 9 and 12; these residues are denoted by X, Y, Z, -Y, -X and -Z. The invariant Glu or Asp at position 12 provides two oxygens for liganding Ca (bidentate ligand). The basic structural/functional unit of EF-hand proteins is usually a pair of EF-hand motifs that together form a stable four-helix bundle domain. The pairing of EF-hand enables cooperativity in the binding of Ca2+ ions. We list below the proteins which are known to contain EF-hand regions. For each type of protein we have indicated between parenthesis the total number of EF-hand regions known or supposed ...
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Calcium binding protein information including symptoms, causes, diseases, symptoms, treatments, and other medical and health issues.
S100 calcium-binding protein A11 (S100A11) is a protein that in humans is encoded by the S100A11 gene. The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A11 is localized in the cytoplasm of resting human keratinocytes in vitro. S100A11, along with all 13 members of the S100 family, are located as a cluster on chromosome 1q21. The protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Suppression of S100A11 by small interfering RNA caused cells to apoptosis, and overexpression of S100A11 has been found to inhibit apoptosis in tumor cells. Furthermore, the knock-down of S100A11 via siRNA reduces the ...
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Haiech, J.; Heizmann, C. W.; Krebs, J.: The 10th European symposium on calcium-binding proteins in normal and transformed cells. 10th European Symposium on Calcium-Binding Proteins in Normal and Transformed Cells Preface, Leuven, BELGIUM, September 17, 2008 - September 20, 2008. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 1793 (6), pp. 931 - 932 (2009 ...
Ca2+ signaling in neurons is characterized by highly restricted and dynamic gradients called Ca2+ waves, spikes, transients and puffs depending upon their corresponding spatial and temporal features. Based on this strict segmentation the Ca2+ ion provides a versatile basis for complex signaling in neuronal subcompartments with a spatial resolution of micro- and nanodomains. The multitude of Ca2+-regulated processes requires specialized downstream processing machinery, translating the Ca2+ signal into alterations of cellular processes. The broad range of different Ca2+-triggered phenomena in neurons, ranging from neurotransmission to gene expression, is reflected by the existence of a multitude of different Ca2+-binding proteins (CaBPs) from which numerous belong to the EF-hand super-family. EF-hand proteins can be subdivided into Ca2+ buffer and Ca2+ sensor proteins. Whereas the first group has a very high affinity for Ca2+, exhibits little conformational change in the Ca2+-bound state and is thought to
Heart failure (HF) represents the common endpoint of many different kinds of cardiopulmonary diseases. Essentially, loss of myocardium triggers a sequence of molecular, cellular and physiological responses leading to ventricular remodelling and the inability of the ventricle to maintain an output sufficient for the metabolic requirements of the tissues of the body [1]. Although these responses may be viewed as compensatory in nature, many of them are or become counterregulatory and lead to long-term adverse effects [1]. Thus, novel insight into the regulatory mechanisms that contribute to the compensation and subsequent decompensation is urgently needed to broaden our knowledge about HF and to develop therapeutic strategies that can prevent the progressive deterioration of cardiac function and ultimately avoid this disease. S100 proteins constitute the largest subfamily of EF-hand Ca-binding proteins. Several biological activities, such as the regulation of myocardial and skeletal muscle contractility,
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. [provided by RefSeq, Jul 2008] ...
Montaville, P.; Dai, Y.; Cheung, C. Y.; Giller, K.; Becker, S.; Michalak, M.; Webb, S. E.; Miller, A.; Krebs, J.: Nuclear translocation of the calcium-binding protein ALG-2 induced by the RNA-binding protein RBM22. Biochimica et Biophysica Acta-Molecular Cell Research 1763 (11 Sp. Iss. SI), S. 1335 - 1343 (2006 ...
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
Contact us today for a free consultation with the scientific team and discover how Creative Biogene can be a valuable resource and partner for your organization.. ...
Contact us today for a free consultation with the scientific team and discover how Creative Biogene can be a valuable resource and partner for your organization.. ...
Recombinant human S100B Calcium-Binding Protein, Western Blot Control protein - 230-00002-WBC. Liquid . Expression system is Escherichia coli (E.coli).
S100A8 and S100A9 are cytosolic calcium-binding proteins of 8kDa and 14kDa respectively that form a heterodimer. S100A8 and S100A9 are
S100A8 and S100A9 are cytosolic calcium-binding proteins of 8kDa and 14kDa respectively that form a heterodimer. S100A8 and S100A9 are
... purification and characterization of calcium-triggered luciferin-binding protein of Renilla reniformis". Protein expression and ... Involvement of a calcium-triggered luciferin binding protein". Biochemistry. 13 (6): 1195-200. doi:10.1021/bi00703a602. PMID ... Unlike photoproteins which stably bind coelenterazine and emit light upon addition of calcium, coelenterazine is normally bound ... and is coupled with a closely interacting green fluorescent protein (RrGFP), and a Ca++ activated luciferin binding protein ( ...
... is a calcium-binding protein of the sarcoplasmic reticulum. The protein helps hold calcium in the cisterna of the ... The release of calsequestrin-bound calcium (through a calcium release channel) triggers muscle contraction. The active protein ... Each molecule of calsequestrin can bind 18 to 50 Ca2+ ions.[1] Sequence analysis has suggested that calcium is not bound in ... When calcium binds there is a structural change whereby the alpha-helical content of the protein increases from 3 to 11%.[2] ...
metal ion binding. • calcium-dependent protein binding. • protein binding. • RAGE receptor binding. • identical protein binding ... calcium ion binding. • S100 protein binding. • protein homodimerization activity. • zinc ion binding. • ... S100 calcium-binding protein B (S100B) is a protein of the S-100 protein family. ... "Entrez Gene: S100B S100 calcium binding protein B".. *^ Verma N, Karmakar M, Singh KP, Smita S (February 2013). "Structural and ...
"Calcium regulation of Ndr protein kinase mediated by S100 calcium-binding proteins". EMBO J. 17 (20): 5913-22. doi:10.1093/ ... 2004). "Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation ... protein kinase by the hMOB1 protein". J. Biol. Chem. 279 (34): 35228-35. doi:10.1074/jbc.M404542200. PMID 15197186. Jin J, ... Serine/threonine-protein kinase 38 is an enzyme that in humans is encoded by the STK38 gene. GRCh38: Ensembl release 89: ...
S100 calcium-binding protein P (S100P) is a protein that in humans is encoded by the S100P gene. The protein encoded by this ... This protein, in addition to binding Ca2+, also binds Zn2+ and Mg2+. This protein may play a role in the etiology of prostate ... S100P S100 calcium binding protein P". Koltzscher M, Neumann C, König S, Gerke V (Jun 2003). "Ca2+-dependent binding and ... binding protein from human placenta. cDNA cloning, recombinant protein expression and Ca2+ binding properties". Eur. J. Biochem ...
The encoded protein is a secreted calcium-binding protein which is found in the cytoplasm. It is related to calbindin D-28K and ... calcium ion binding. • metal ion binding. Cellular component. • cytoplasm. • extracellular region. • neuron projection. • ... This protein is thought to be involved in potassium chloride-stimulated calcium flux and cell proliferation.[6] This protein ... SCGN, CALBL, DJ501N12.8, SECRET, SEGN, setagin, secretagogin, EF-hand calcium binding protein. ...
calcium ion binding. • extracellular matrix constituent conferring elasticity. • protein complex binding. • extracellular ... integrin binding. • protein binding. • hormone activity. • heparin binding. • identical protein binding. • macromolecular ... ß protein binds with latency associated protein (LAP) at the N-terminal propertied and one of three latent TGF-ß binding ... Handford PA (December 2000). "Fibrillin-1, a calcium binding protein of extracellular matrix". Biochimica et Biophysica Acta. ...
Celio, Marco R.; Pauls, Thomas; Schwaller, Beat (1996). Guidebook to the calcium-binding proteins. Oxford: Sambrook & Tooze ... and calprotectin is able to bind a total of four calcium ions per dimer or eight calcium ions per tetramer. Calcium binding ... Zinc can bind at two sites within the calprotectin dimer, and this can occur in the absence of calcium. Calcium, however, ... Manganese or iron are bound by the calprotectin dimer at this His6 site. Zinc can be bound to either of the sites that form at ...
... bind calcium and phospholipids such as lipocortin. Many other proteins bind calcium, although binding calcium may not be ... Many of the proteins that calmodulin binds are unable to bind calcium themselves, and use calmodulin as a calcium sensor and ... bind only to the Ca2+-bound protein, whereas some proteins, such as NaV channels and IQ-motif proteins, also bind to calmodulin ... an abbreviation for calcium-modulated protein) is a multifunctional intermediate calcium-binding messenger protein expressed in ...
The protein has two approximately symmetrical domains, separated by a flexible "hinge" region. Binding of calcium causes a ... Troponin, along with actin and tropomyosin, is the protein complex to which calcium binds to trigger the production of muscular ... For instance, at least 1000 human proteins (out of ~20,000) contain zinc-binding protein domains[3] although there may be up to ... Lead(II) Binding in Natural and Artificial Proteins". In Astrid S, Helmut S, Sigel RK. Lead: Its Effects on Environment and ...
Oncomodulin is a high-affinity calcium ion-binding protein. It belongs to the superfamily of calmodulin proteins, also known as ... Belkacemi L, Simoneau L, Lafond J (2003). "Calcium-binding proteins: distribution and implication in mammalian placenta". ... 1987). "A complete complementary DNA for the oncodevelopmental calcium-binding protein, oncomodulin". J. Biol. Chem. 262 (11): ... 1992). "The genes for the highly homologous Ca(2+)-binding proteins oncomodulin and parvalbumin are not linked in the human ...
CHP: Calcium binding protein P22. *CHSY1: Chondroitin sulfate synthase 1. *CLK3: CDC like kinase 3 ... KATNBL1: encoding protein KATNBL1. *LARP6 encoding protein La-related protein 6 also known as acheron or La ribonucleoprotein ... MIR627: encoding protein MicroRNA 627. *NIPA2: encoding protein Non-imprinted in Prader-Willi/Angelman syndrome region protein ... PDCD7: encoding protein Programmed cell death protein 7. *PML: promyelocytic leukemia protein (involved in t(15,17) with ...
... is a calcium binding protein. Calponin tonically inhibits the ATPase activity of myosin in smooth muscle. ... Phosphorylation of calponin by a protein kinase, which is dependent upon calcium binding to calmodulin, releases the calponin's ... Calmodulin, when activated by calcium may bind weakly to the CH domain and inhibit calponin binding with α-actin. Calponin is ... It is a binding protein and is made up of three domains. These domains in order of appearance are Calponin Homology (CH), ...
S100A7, S100 calcium-binding protein A7. This disambiguation page lists articles associated with the title Psoriasin. If an ...
Does the visual system of the flying fox resemble that of primates? The distribution of calcium-binding proteins in the primary ... A molecular perspective on mammalian evolution from the gene encoding interphotoreceptor retinoid binding protein, with ...
Members of the KCNIP family are small calcium binding proteins. They all have EF-hand-like domains, and differ from each other ... "Entrez Gene: KCNIP1 Kv channel interacting protein 1". Burgoyne RD (2007). "Neuronal calcium sensor proteins: generating ... Lin YL, Chen CY, Cheng CP, Chang LS (2004). "Protein-protein interactions of KChIP proteins and Kv4.2". Biochem. Biophys. Res. ... 2001). "Different effects of the Ca(2+)-binding protein, KChIP1, on two Kv4 subfamily members, Kv4.1 and Kv4.2". FEBS Lett. 499 ...
Strynadka, N. C. J.; James, M. N. G. (1989). "Crystal Structures of the Helix-Loop-Helix Calcium-Binding Proteins". Annual ... Her certificate of election reads: Natalie Strynadka is a pioneer in the study of proteins and protein assemblies essential to ... "Structural analysis of the essential self-cleaving type III secretion proteins EscU and SpaS". Nature. 453 (7191): 124-7. ... "Structural and kinetic characterization of a beta-lactamase-inhibitor protein". Nature. 368 (6472): 657-60. Bibcode:1994Natur. ...
Strynadka, N C J; James, M N G (1989). "Crystal Structures of the Helix-Loop-Helix Calcium-Binding Proteins". Annual Review of ... Read, Randy John (1986). X-ray crystallographic studies on serine proteinases and their protein inhibitors (PhD thesis). ...
Members of the KCNIP family are small calcium binding proteins. They all have EF-hand-like domains, and differ from each other ... "Entrez Gene: KCNIP2 Kv channel interacting protein 2". Burgoyne RD (Mar 2007). "Neuronal calcium sensor proteins: generating ... Lin YL, Chen CY, Cheng CP, Chang LS (Aug 2004). "Protein-protein interactions of KChIP proteins and Kv4.2". Biochemical and ... Kv channel-interacting protein 2 also known as KChIP2 is a protein that in humans is encoded by the KCNIP2 gene. This gene ...
Members of the KCNIP family are small calcium binding proteins. They all have EF-hand-like domains, and differ from each other ... "Entrez Gene: KCNIP4 Kv channel interacting protein 4". Burgoyne RD (2007). "Neuronal calcium sensor proteins: generating ... "Effects of metal-binding properties of human Kv channel-interacting proteins on their molecular structure and binding with ... Kv channel-interacting protein 4 is a protein that in humans is encoded by the KCNIP4 gene. This gene encodes a member of the ...
Actin binding proteins are in turn regulated by calcium ions; hence, calcium ions are very important in the sol-gel conversion ... Cytoplasm consist largely of actin and actin is regulated by actin binding proteins. ... Inside the amoeba, there are proteins that can be activated to convert the gel into the more liquid sol state. ... Finally, continuous application of stress on the old focal adhesion sites could result in the calcium-induced activation of ...
"Entrez Gene: S100 calcium binding protein A7A". Wolf, R; Voscopoulos, CJ; FitzGerald, PC; Goldsmith, P; Cataisson, C; Gunsior, ... The S100 calcium-binding protein mS100a7a15 is the murine ortholog of human S100A7 (Psoriasin) and human S100A15 (Koebnerisin ... S100 proteins are a diverse calcium-binding family that mediate fundamental cellular and extracellular processes including cell ... motifs corresponding to the for S100 proteins typical conserved C-terminal and a variant N-terminal EF-hand calcium binding ...
S100 calcium binding protein A14 (S100A14) is a protein that in humans is encoded by the S100A14 gene. This gene encodes a ... "Entrez Gene: S100 calcium binding protein A14". Wang HY, Zhang JY, Cui JT, Tan XH, Li WM, Gu J, Lu YY (Jan 2010). "Expression ... rationale for a new nomenclature of the S100 calcium-binding protein family". Genomics. 25 (3): 638-43. doi:10.1016/0888-7543( ... member of the S100 protein family which contains an EF-hand motif and binds calcium. The gene is located in a cluster of S100 ...
S100 calcium-binding protein A15 (S100A15), also known as koebnerisin and S100 calcium-binding protein A7A (S100A7A), is a ... "Entrez Gene: S100 calcium binding protein A7A". Wolf R, Mirmohammadsadegh A, Walz M, Lysa B, Tartler U, Remus R, Hengge U, ... S100 proteins are a diverse calcium-binding family that regulate fundamental cellular and extracellular processes including ... "Entrez Gene: S100a7a S100 calcium binding protein". Briso EM, Guinea-Viniegra J, Bakiri L, Rogon Z, Petzelbauer P, Eils R, Wolf ...
Each tropomyosin molecule has a smaller calcium-binding protein called troponin bound to it. All thin filaments are attached to ... Troponin and the associated tropomyosin undergo a conformational change after calcium binding and expose the myosin binding ... Active transport moves calcium ions back into the sarcoplasmic reticulum of the muscle fiber. ATP causes the binding between ... Calcium ions are then released from the sarcoplasmic reticulum into the sarcoplasm and subsequently bind to troponin. ...
S100G is a member of the S100 family of calcium-binding proteins. S100G mediates the transport of calcium across the ... Calbindin contains 4 active calcium-binding domains, and 2 modified domains that have lost their calcium-binding capacity. ... Such proteins bound calcium in the micromolar range and were greatly reduced in vitamin D-deficient animals. Expression could ... They were originally described as vitamin D-dependent calcium-binding proteins in the intestine and kidney in the chick and ...
Grabarek Z (Jun 2006). "Structural basis for diversity of the EF-hand calcium-binding proteins". Journal of Molecular Biology. ... Harris SP, Lyons RG, Bezold KL (Mar 2011). "In the thick of it: HCM-causing mutations in myosin binding proteins of the thick ... Perturbing the calcium binding region of RLC through site-directed mutagenesis (D47A) decreased tension and stiffness in ... The N-terminal EF-hand domain of RLC binds calcium/magnesium at activating concentrations, however the dissociation rate is too ...
This gene encodes a divergent member of the annexin protein family in which all four homologous type II calcium-binding sites ... each of which includes a type II calcium-binding site. The calcium-binding sites are required for annexins to aggregate and ... The annexins are a family of calcium-dependent phospholipid-binding proteins. Members of the annexin family contain 4 internal ... and lipid binding proteins". FEBS Letters. 546 (2-3): 359-64. doi:10.1016/S0014-5793(03)00634-3. PMID 12832069. S2CID 13433731 ...
... S100 calcium binding protein A8". Schäfer BW, Heizmann CW (1996). "The S100 family of EF-hand calcium-binding proteins: ... S100 calcium-binding protein A8 (S100A8) is a protein that in humans is encoded by the S100A8 gene. It is also known as ... The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 ... Dorin JR, Emslie E, van Heyningen V (1991). "Related calcium-binding proteins map to the same subregion of chromosome 1q and to ...
S100 calcium-binding protein A9 (S100A9) also known as migration inhibitory factor-related protein 14 (MRP14) or calgranulin B ... S100-A9 is a member of the S100 family of proteins containing 2 EF hand calcium-binding motifs. S100 proteins are localized in ... "Entrez Gene: S100A9 S100 calcium binding protein A9". Vogl T, Tenbrock K, Ludwig S, Leukert N, Ehrhardt C, van Zoelen MA, ... Dorin JR, Emslie E, van Heyningen V (1991). "Related calcium-binding proteins map to the same subregion of chromosome 1q and to ...
Zhou W, Resh MD (1997). "Differential membrane binding of the human immunodeficiency virus type 1 matrix protein". J. Virol. 70 ... Kobayashi M, Takamatsu K, Saitoh S, Noguchi T (1993). "Myristoylation of hippocalcin is linked to its calcium-dependent ... 1985). "Amino terminal myristylation of the protein kinase p60src, a retroviral transforming protein". Science. 227 (4685): 427 ... "Identification of a membrane-binding domain within the amino-terminal region of human immunodeficiency virus type 1 Gag protein ...
Protein binding. 60%. Metabolism. Hepatic via CYP3A4. Elimination half-life. 1.8 ± 0.4 hours. ... As a result, structural proteins, resulting from polypeptide products of gag and gag-pol genes, that are necessary for the HIV ...
Once interferon has bound to its receptors on the neighbouring cell, the signalling proteins STAT1 and STAT2 are activated and ... or calcium hypochlorite (bleaching powder), and other suitable disinfectants may be used at appropriate concentrations.[81][115 ... EBOV's V24 protein blocks the production of these antiviral proteins by preventing the STAT1 signalling protein in the ... This processing appears to allow the virus to bind to cellular proteins enabling it to fuse with internal cellular membranes ...
... by G protein-coupled receptors that can activate or inhibit the NALCN channels depending on the neurotransmitter that binds the ... Calcium-sensitive dyes have shown that internal concentrations of calcium increase during bursts. The activation of different ... Other research has also suggested that calcium flow through N-type calcium channels is essential for normal breathing, and is ... voltage gated calcium channels become activated and calcium is able to flow into the cell which usually leads to the release of ...
Greater than 99% of circulating thyroid hormones are bound to plasma proteins including thyroxine-binding globulin, ... T4 and T3 bind to thyroid receptor proteins in the cell nucleus and cause metabolic effects through the control of DNA ... transthyretin (previously called 'thyroxine-binding prealbumin'), and albumin.[15] Only free hormone is metabolically active.[ ... transcription and protein synthesis.[20] Like its naturally secreted counterpart, levothyroxine is a chiral compound in the L- ...
This results in a series of unstable intermediates, the last of which binds stronger to a G protein in the membrane, called ... This leads to a decrease in the influx of calcium ions into the cell and thus the intracellular calcium ion concentration falls ... A decrease in the intracellular calcium concentration means that less glutamate is released via calcium-induced exocytosis to ... The membranous photoreceptor protein opsin contains a pigment molecule called retinal. In rod cells, these together are called ...
... s bind to and activate androgen receptors (ARs) to mediate most of their biological effects. ... NMDA induces a calcium flux that allows for synaptic plasticity which is crucial for AHN. ... "G protein-coupled receptors: extranuclear mediators for the non-genomic actions of steroids". Int J Mol Sci. 15 (9): 15412-25 ... Dihydrotestosterone (DHT) is a metabolite of testosterone, and a more potent androgen than testosterone in that it binds more ...
Colabufo NA, Berardi F, Abate C, Contino M, Niso M, Perrone R (2006). „Is the sigma2 receptor a histone binding protein?". ... Cell Calcium. 40 (1): 23-8. PMID 16687172. doi:10.1016/j.ceca.2006.03.004.. ... The sigma-2 receptor agonist PB28 inhibits calcium release from the endoplasmic reticulum of SK-N-SH neuroblastoma cells". ...
Calcium Scandium Titanium Vanadium Chromium Manganese Iron Cobalt Nickel Copper Zinc Gallium Germanium Arsenic Selenium Bromine ... The balance between potassium and sodium is maintained by ion transporter proteins in the cell membrane.[231] The cell membrane ... Sodium tetraphenylborate can also be classified as an organosodium compound since in the solid state sodium is bound to the ... Similar solutions are formed by the heavy divalent alkaline earth metals calcium, strontium, barium, as well as the divalent ...
Beta carotene, vitamin K, vitamin C, an calcium is rowth in kail. Kail is a soorce o twa carotenoids, lutein an zeaxanthin.[1] ... "Steam cooking significantly improves in vitro bile acid binding of collard greens, kale, mustard greens, broccoli, green bell ...
The dye molecules bind to proteins including wool (keratin) to form a protein-dye complex. The formation of the complex ... The binding of the dye to a protein causes a shift in the absorbance maximum of the dye from 465 to 595 nm. The increase of ... On binding to a protein the negatively charged Coomassie Brilliant Blue G-250 dye molecule will give an overall negative charge ... Under the acid conditions the dye is normally a brownish colour but on binding to the protein the blue form of the dye is ...
Small acid-soluble proteins (SASPs) are found in endospores. These proteins tightly bind and condense the DNA, and are in part ... Calcium dipicolinate, the calcium salt of dipicolinic acid, is incorporated into the forespore during this time. The ... Up to 20% of the dry weight of the endospore consists of calcium dipicolinate within the core, which is thought to stabilize ... In Bacillus subtilus endospores, the spore coat is estimated to contain more than 70 coat proteins, which are organized into an ...
... it was thought that the sole important retinoid delivery pathway to tissues involved retinol bound to retinol-binding protein ( ... blood calcium test. *cholesterol test. *liver function test. *blood test for vitamin A ... It is likely to exert this effect by binding to specific nuclear receptors (members of the retinoic acid receptor or retinoid X ... retinoic acid bound to albumin, water soluble β-glucuronides of retinol and retinoic acid, and provitamin A carotenoids.[29] ...
Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing ... Calcium/calmodulin-regulated capping of the barbed ends of actin filaments". J. Biol. Chem. 271 (14): 7986-91. doi:10.1074/jbc. ... Definition of the calmodulin-binding domain and sites of phosphorylation by protein kinases A and C". J. Biol. Chem. 271 (41): ... Alpha-adducin is a protein that in humans is encoded by the ADD1 gene. Adducins are a family of cytoskeleton proteins encoded ...
PDZ domain binding. • cadherin binding. • peptidase activity. • beta-catenin binding. • protein binding. • calcium channel ... positive regulation of protein binding. • positive regulation of protein import into nucleus, translocation. • Notch receptor ... protein processing. • protein maturation. • myeloid dendritic cell differentiation. • autophagy. • protein glycosylation. • ... a calcium-binding protein that interacts with the presenilins and regulates the levels of a presenilin fragment". Nat. Med. 4 ( ...
... protein GP41 of HIV-1 inhibits distinct lymphocyte activation pathways dependent on protein kinase C and intracellular calcium ... 1992). "Overlapping but nonidentical binding sites on CD2 for CD58 and a second ligand CD59.". Science 256 (5065): 1805-7. PMID ... Wilkins A, Yang W, Yang J (2003). "Structural biology of the cell adhesion protein CD2: from molecular recognition to protein ... 1990). "Immunoregulatory effect of a synthetic peptide corresponding to a region of protein p24 of HIV.". Folia Biol. (Praha) ...
protein binding. • alpha-actinin binding. • voltage-gated calcium channel activity. • voltage-gated calcium channel activity ... When depolarization occurs, calcium ions flow through the channel and some bind to Calmodulin. The Calcium/Calmodulin binding ... When calcium ions bind to calmodulin, which in turn binds to a Cav1.2 channel, it allows the Cav1.2 channels within a cluster ... calcium-mediated signaling using extracellular calcium source. • transmembrane transport. • calcium ion transport. • regulation ...
SHCBP1: encoding protein SHC SH2 domain-binding protein 1. *SLZ1: encoding protein SLX1 structure-specific endonuclease subunit ... C16orf96: encoding protein C16orf96, or chromosome 16 open reading frame 96,. *CARHSP1: Calcium-regulated heat stable protein 1 ... UNKL: encoding protein RING finger protein unkempt-like. *VAT1L: encoding protein Vesicle amine transport protein 1 homolog (T ... LINC00273 encoding protein Long intergenic non-protein coding RNA 273. *LOC124220: encoding protein Zymogen granule protein 16 ...
G-protein coupled receptor activity. • signal transducer activity. • G-protein alpha-subunit binding. ... positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling ... regulation of protein phosphorylation. • histone H3-S10 phosphorylation. • G-protein coupled receptor signaling pathway. • ... protein import into nucleus. • positive regulation of potassium ion transport. • response to amphetamine. • научение. • пищевое ...
"Momordica charantia and its novel polypeptide regulate glucose homeostasis in mice via binding to insulin receptor". Journal of ...
Wnt-protein binding. • protein binding. • protein kinase binding. • ubiquitin protein ligase binding. • transmembrane signaling ... Wnt signaling pathway, calcium modulating pathway. • Wnt signaling pathway, planar cell polarity pathway. • beta-catenin ... gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD5 protein is believed ... Katoh Y, Katoh M (2007). "Conserved POU-binding site linked to SP1-binding site within FZD5 promoter: Transcriptional ...
... thyroxine-binding globulin (TBG)) tae form ligand-protein complexes. Some hormones are completely active whan released intae ... For instance, serum calcium concentration affects parathyroid hormone synthesis; bluid succar (serum glucose concentration) ... Whan a hormone binds tae the receptor, it results in the activation o a seegnal transduction pathwey that teepically activates ... Hormones hae diverse chemical structurs, mainly o 3 clesses: eicosanoids, steroids, an amino acid/protein derivatives (amines, ...
At least one mechanism by which these mutations cause this syndrome is by loss of its Rab binding domain. This protein is ... calcium and L-carnitine, this problem, is known as Fanconi-type renal tubular dysfunction.[medical citation needed] This ... "Crystal structure of the Rab binding domain of OCRL1 in complex with Rab8 and functional implications of the OCRL1/Rab8 module ...
Protein C (inhibits V, VIII)/Protein S (cofactor for protein C). *Protein Z (inhibits X) ... Carbasalate calcium. *Indobufen. *Triflusal. Thromboxane inhibitors. *Thromboxane synthase inhibitors *Dipyridamole (+ aspirin) ...
Protein binding. 50% (active metabolite). Metabolism. Hepatic (CYP2D6- and 3A4-mediated). Elimination half-life. 7-8 hours ( ...
lipid binding. Cellular component. • cytoplasm. • cyclin-dependent protein kinase 5 holoenzyme complex. • plasma membrane. • ... p35 and p39 interact with the alpha-subunit of Ca2+/calmodulin-dependent protein kinase II and alpha-actinin-1 in a calcium- ... positive regulation of protein kinase activity. • regulation of cyclin-dependent protein serine/threonine kinase activity. • ... Agarwal-Mawal A, Paudel HK (2001). "Neuronal Cdc2-like protein kinase (Cdk5/p25) is associated with protein phosphatase 1 and ...
cAMP binds to and releases an active form of protein kinase A (PKA). Next, PKA phosphorylates phosphorylase kinase, which, in ... The increased calcium availability binds to the calmodulin subunit and activates glycogen phosphorylase kinase. Glycogen ... Residues 397-437 form this structure, which allows the protein to covalently bind to the glycogen chain a full 30 Å from the ... Binding of AMP at this site, corresponding in a change from the T state of the enzyme to the R state, results in small changes ...
receptor binding. • neurotrophin TRKB receptor binding. • growth factor activity. • GO:0001948 protein binding. ... triggering a calcium influx. Through a protein signaling cascade requiring Erk, CaM KII/IV, PI3K, and PLC, NMDA receptor ... Binding proteins: IGFBP (1, 2, 3, 4, 5, 6, 7). *Cleavage products/derivatives with unknown target: Glypromate (GPE, (1-3)IGF-1) ... positive regulation of receptor binding. • regulation of protein localization to cell surface. • regulation of receptor ...
SOD1 is an extremely stable protein. In the holo form (both copper and zinc bound) the melting point is , 90 °C. In the apo ... which binds both copper and zinc), Fe and Mn types (which bind either iron or manganese), and the Ni type (which binds nickel ... However, ingestion of SOD bound to wheat proteins could improve its therapeutic activity, at least in theory.[52] ... identified a protein that later became known as superoxide dismutase as an indophenol oxidase by protein analysis of starch ...
... meeting in the splendid environment of Crans-Montana in Switzerland a limited number of people around the subject of calcium an ... Calcium Binding Proteins in Non-Muscle Tissues. * Front Matter Pages 91-91 ... the other sessions were devoted to calmodulin-related calcium binding proteins in muscle and non- muscle tissues and to some ... Calcium Binding to Troponin C and the Regulation of Muscle Contraction: a Comparative Approach ...
Among the many calcium-binding proteins in the nervous system, parvalbumin, calbindin-D28K and calretinin are particularly ... Calcium-binding proteins in the nervous system Trends Neurosci. 1992 Aug;15(8):303-8. doi: 10.1016/0166-2236(92)90081-i. ... Among the many calcium-binding proteins in the nervous system, parvalbumin, calbindin-D28K and calretinin are particularly ... or merely act as buffers to modulate cytosolic calcium transients, they are valuable markers of neuronal subpopulations for ...
calcium-dependent membrane binding protein annexin I, partial [Gallus gallus] calcium-dependent membrane binding protein ... calcium-dependent membrane binding protein annexin I, partial [Gallus gallus]. GenBank: AAB28036.2 ... Transcript/Protein Information [PANTHER Classification System] Transcript/Protein Information. PANTHER Classification System ... RefSeq protein isoforms See 2 reference sequence protein isoforms for the ANXA1 gene. ...
S100Z S100 calcium binding protein Z [Gallus gallus] S100Z S100 calcium binding protein Z [Gallus gallus]. Gene ID:431598 ... S100 calcium binding protein Zprovided by CGNC. Primary source. CGNC:11139 See related. Ensembl:ENSGALG00000014989 Gene type. ... S100Z S100 calcium binding protein Z [ Gallus gallus (chicken) ] Gene ID: 431598, updated on 14-Feb-2019 ... S100Z is a member of the S100 domain family within the EF-hand Ca2+-binding proteins superfamily. Note that the S-100 hierarchy ...
... is a powerful tool for examining the metal coordination of the side chain COO groups of Glu and Asp on Ca-binding... ... Calcium-binding Proteins. Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or ... Calcium-binding proteins that are found in DISTAL KIDNEY TUBULES, INTESTINES, BRAIN, and other tissues where they bind, buffer ... They contain EF HAND MOTIFS and undergo conformational changes upon calcium-binding. Neuronal calcium-sensor proteins interact ...
Role of the calcium-binding protein parvalbumin in short-term synaptic plasticity. Olivier Caillard, Herman Moreno, Beat ... Calcium-binding proteins such as parvalbumin (PV), calretinin, and calbindin D28k are important modulators of intracellular ... Role of the calcium-binding protein parvalbumin in short-term synaptic plasticity ... Role of the calcium-binding protein parvalbumin in short-term synaptic plasticity ...
Compare S100 calcium binding protein B Proteins from Cell Sciences from leading suppliers on Biocompare. View specifications, ... S100 calcium binding protein B Proteins from Cell Sciences. Recombinant proteins for S100 calcium binding protein B are ... S-100 calcium-binding protein, beta chain, S-100 protein subunit beta, S100 calcium-binding protein, beta (neural). This ... Your search returned 2 Proteins S100 calcium binding protein B Recombinant Proteins across 1 supplier. ...
In addition, the group of proteins isolated includes another calcium-binding protein of 20 kd, as well as GTP-binding proteins ... The two major proteins isolated (32.5 kd and 67 kd) are calcium-binding proteins and have been identified as annexins V and VI ... Annexins V and VI: major calcium-dependent atrial secretory granule-binding proteins.. A F Doubell, A J Bester, G Thibault ... We have isolated a group of atrial proteins by affinity chromatography that bind to atrial specific granules in a calcium- ...
Rabbit polyclonal Calcium binding protein P22 antibody. Validated in WB, IHC, ICC/IF and tested in Mouse, Rat, Human. Immunogen ... Anti-Calcium binding protein P22 antibody. See all Calcium binding protein P22 primary antibodies. ... Recombinant full length protein corresponding to Human Calcium binding protein P22 aa 2-195.. Sequence: ... All lanes : Anti-Calcium binding protein P22 antibody (ab223115) at 1/500 dilution. Lane 1 : Rat kidney lysate. Lane 2 : Mouse ...
Ab125667 is a full length protein produced in Escherichia coli and has been validated in SDS-PAGE… ... Buy our Recombinant Human S100 Calcium Binding Protein A13. ... S100 calcium binding protein A13. *S100 calcium-binding protein ... Recombinant Human S100 Calcium Binding Protein A13 See all S100 Calcium Binding Protein A13 proteins and peptides. ... Binds two calcium ions per subunit. Binds one copper ion. Binding of one copper ion does not interfere with calcium binding. ...
Serine-Aspartate Repeat Protein D Increases Staphylococcus aureus Virulence and Survival in Blood Fatemeh Askarian, Satoshi ... ITIH4 (Inter-Alpha-Trypsin Inhibitor Heavy Chain 4) Is a New Acute-Phase Protein Isolated from Cattle during Experimental ...
Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They ... Calcium-Binding Proteins. Subscribe to New Research on Calcium-Binding Proteins Proteins to which calcium ions are bound. They ... Amino Acids, Peptides, and Proteins*Proteins: 90489*Carrier Proteins: 11456*Calcium-Binding Proteins: 268*S100 Proteins: 2374 ... or activator proteins. They typically contain EF HAND MOTIFS.. Also Known As: Calcium Binding Proteins; Binding Proteins, ...
Now, Boston scientists have found that thisoccurs because mutations in the LDL receptor prevent the protein from folding into ... Protein Folding And Calcium Binding Defects Account For Errors In Familial Hypercholesterolemia. Whitehead Institute for ... Protein Folding And Calcium Binding Defects Account For Errors In Familial Hypercholesterolemia ... All proteins are strings of amino acids that must fold into a unique shape to perform their function. 3Knowing that a protein ...
... but they did not affect enzymatic activity in the presence of calcium alone. Troponin C shows little effect on the cyclase ... Interaction of calcium-binding proteins with calmodulin-dependent guanylate cyclase in Tetrahymena plasma membrane Comp Biochem ... but not by calcium. Both calmodulins from Tetrahymena and bovine brain elicited stimulation of heart phosphodiesterase, while ... but they did not affect enzymatic activity in the presence of calcium alone. Troponin C shows little effect on the cyclase ...
... likely via inhibition of the inactivation of voltage-gated calcium channel of type 1.3 (Cav1.3) in the IHCs (PubMed:28183797). ... of the calcium-binding region(s) within the protein. One common calcium-binding motif is the EF-hand, but other calcium-binding ... Calcium-binding protein 2Add BLAST. 219. Amino acid modifications. Feature key. Position(s). DescriptionActions. Graphical view ... calcium channel regulator activity Source: UniProtKB. *calcium ion binding Source: ProtInc ,p>Traceable Author Statement,/p> ,p ...
... of the calcium-binding region(s) within the protein. One common calcium-binding motif is the EF-hand, but other calcium-binding ... Calcium-binding protein 2Imported. ,p>Information which has been imported from another database using automatic procedures.,/p ... tr,G3XA16,G3XA16_MOUSE Calcium-binding protein 2 OS=Mus musculus OX=10090 GN=Cabp2 PE=4 SV=1 ... Protein predictedi ,p>This indicates the type of evidence that supports the existence of the protein. Note that the protein ...
Browse our Calcium-binding-protein-P22 RNAi catalog backed by our Guarantee+. ... Calcium-binding-protein-P22 RNAi available through Novus Biologicals. ... calcium binding protein P22 RNAi, Calcium-binding protein CHP RNAi, calcium-binding protein p22 RNAi, SLC9A1 binding protein ... We offer Calcium-binding-protein-P22 RNAi for use in common research applications. Each Calcium-binding-protein-P22 RNAi is ...
The results suggest a role for vitamin D--dependent calcium binding protein in intracellular calcium metabolism rather than a ... cells in these tubular segments were negative for the calcium binding protein. Subcellularly, the protein was found in the ... Vitamin D--dependent calcium binding protein: immunocytochemical localization in chick kidney. By J Roth, B Thorens, W Hunziker ... Vitamin D--dependent calcium binding protein: immunocytochemical localization in chick kidney. By J Roth, B Thorens, W Hunziker ...
Expression of calcium-binding proteins in pathways from the nucleus of the basal optic root to the cerebellum in pigeons - ... 2002). New functions for old proteins: The role of the calcium-binding proteins calbindin D-28k, calretinin and parvalbumin ... 1991). Calretinin, a neuronal calcium binding protein, inhibits phosphorylation of a 39 kDa synaptic membrane protein from rat ... Expression of calcium-binding proteins in pathways from the nucleus of the basal optic root to the cerebellum in pigeons. * ...
Calcium binding proteins (CaBPs) such as calbindin D28-k, parvalbumin, and calretinin are able to bind Ca2+ with high affinity ... K. G. Baimbridge, M. R. Celio, and J. H. Rogers, "Calcium-binding proteins in the nervous system," Trends in Neurosciences, vol ... Co-existence with other calcium binding proteins and GABA," Neuroscience, vol. 48, no. 1, pp. 29-43, 1992. View at Publisher · ... O. Caillard, H. Moreno, B. Schwaller, I. Llano, M. R. Celio, and A. Marty, "Role of the calcium-binding protein parvalbumin in ...
Calcium-binding proteins play an important role in a variety of vital biological processes, ranging from blood clotting and ... signal transduction in cells, to attaching proteins to membranes and serving ... calcium-binding properties, and biological activity of calcium-binding proteins in general.. Comprehensive and highly practical ... to attaching proteins to membranes and serving as an integral source of calcium. In Calcium-Binding Protocols-Volume 1: Reviews ...
Calcium-binding proteins are proteins that participate in calcium cell signalling pathways by binding to Ca2+, the calcium ion ... low-affinity calcium-binding protein calsequestrin. Calretinin is another type of Calcium binding protein weighing 29kD. It is ... Calcium-binding proteins have specific domains that bind to calcium and are known to be heterogeneous. One of the functions of ... The cellular regulation of calcium is known as calcium homeostasis. Many different calcium-binding proteins exist, with ...
Molecule model of the calcium-binding protein calmodulin (CaM). This protein is found in all eukaryotic cells, where it ... regulates and modifies the activities of many calcium-binding enzymes. Cellular processes that CaM affects include muscle ... Caption: Calcium-binding protein. Molecule model of the calcium-binding protein calmodulin (CaM). This protein is found in all ... Keywords: artwork, biochemical, biochemistry, biological, biology, black background, calcium-binding, calmodulin, cam, ...
A fluorescent compound exhibiting a high affinity to calcium-binding proteins (CaBP) is provided. The compound, containing ... EN) FLUORESCENT RUTHENIUM COMPOUNDS FOR DETECTING CALCIUM BINDING PROTEINS. (FR) COMPOSÉS DE RUTHÉNIUM FLUORESCENT PERMETTANT ... EN) A fluorescent compound exhibiting a high affinity to calcium-binding proteins (CaBP) is provided. The compound, containing ... FR) La présente invention concerne un composé fluorescent présentant une grande affinité avec les protéines liant le calcium ( ...
S100 calcium binding protein A13), Authors: Carlo Barone, Cinzia Bagalà, Matteo Landriscina. Published in: Atlas Genet ... copper ion binding calcium ion binding protein binding extracellular region extracellular space nucleus nucleolus cytoplasm ... copper ion binding calcium ion binding protein binding extracellular region extracellular space nucleus nucleolus cytoplasm ... S100A13 (S100 calcium binding protein A13). Written. 2008-02. Carlo Barone, Cinzia Bagalà, Matteo Landriscina. ...
S100 calcium binding protein A2), Authors: Christopher J Scarlett, Andrew V Biankin. Published in: Atlas Genet Cytogenet Oncol ... calcium ion binding protein binding cellular_component identical protein binding endothelial cell migration transition metal ... calcium ion binding protein binding cellular_component identical protein binding endothelial cell migration transition metal ... S100A2 calcium-binding protein is characterized by four helices, two distinct calcium-binding EF-hand motifs, a central hinge ...
S100 calcium binding protein B), Authors: M Rosario Fernandez-Fernandez, Alan R Fersht. Published in: Atlas Genet Cytogenet ... Calcium regulation of Ndr protein kinase mediated by S100 calcium-binding proteins.. ... binding innate immune response tau protein binding regulation of neuronal synaptic plasticity calcium-dependent protein binding ... binding innate immune response tau protein binding regulation of neuronal synaptic plasticity calcium-dependent protein binding ...
... [1977] Wasserman, R. H. (Robert Harold) 1926- Division of Biological Sciences. [ ... proceedings of the International Symposium on Calcium-Binding Proteins and Calcium Function in Health and Disease, June 5-9, ... proceedings of the International Symposium on Calcium-Binding Proteins and Calcium Function in Health and Disease, June 5-9, ... proceedings of the International Symposium on Calcium-Binding Proteins and Calcium Function in Health and Disease, June 5-9, ...
This protein, which inhibits muscle contraction in the absence of calcium ions, changes conformation in the presence of calcium ... Developed by: UW Center for Game Science, UW Institute for Protein Design, Northeastern University, Vanderbilt University ...
Browse our S100 calcium binding protein A14 product catalog backed by our Guarantee+. ... S100 calcium binding protein A14 products available through Novus Biologicals. ... PTMs for S100 calcium binding protein A14. Learn more about PTMs related to S100 calcium binding protein A14.. Phosphorylation ... Diseases related to S100 calcium binding protein A14. Discover more about diseases related to S100 calcium binding protein A14. ...
  • They contain EF HAND MOTIFS and undergo conformational changes upon calcium-binding. (bioportfolio.com)
  • One common calcium-binding motif is the EF-hand, but other calcium-binding motifs also exist. (uniprot.org)
  • Small homodimeric protein (98 amino-acids, M.W. 11,47 kDa), with 2 Ca 2+ -binding EF-hands motifs and a positively charged C-terminal region mediating the interaction with specific target molecules. (atlasgeneticsoncology.org)
  • S100A2 calcium-binding protein is characterized by four helices, two distinct calcium-binding EF-hand motifs, a central hinge region and C- and N-terminal variable domains. (atlasgeneticsoncology.org)
  • The protein encoded by S100 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. (antibodies-online.com)
  • A gene on chromosome 1q21 that encodes a member of the S100 family of proteins, which contain 2 EF-hand calcium-binding motifs and as a group regulate cell cycle progression and differentiation and other cellular processes. (thefreedictionary.com)
  • The two proteins are non-integrins containing N-terminal integrin-like Ca(2+)-binding motifs and their structure and function remain to be assigned. (ualg.pt)
  • The encoded protein contains four EF-hand Ca(2+) binding motifs in the N-terminal domain, and localizes to mitochondria. (mybiosource.com)
  • Many Gram-negative bacteria use Type I secretion systems, T1SS, to secrete virulence factors that contain calcium-binding Repeat-in-ToXin (RTX) motifs. (pasteur.fr)
  • Leśniak W, Kuźnicki J. (2006) Binding and functional characteristics of two E-box motifs within the S100A6 (calcyclin) gene promoter. (gov.pl)
  • S100A3 is part of S100 family of proteins containing 2 EF-hand calcium-binding motifs. (neobiolab.com)
  • Among the many calcium-binding proteins in the nervous system, parvalbumin, calbindin-D28K and calretinin are particularly striking in their abundance and in the specificity of their distribution. (nih.gov)
  • Calcium-binding proteins such as parvalbumin (PV), calretinin, and calbindin D 28k are important modulators of intracellular calcium dynamics in neurons ( 10 ) and could therefore influence both facilitation and depression. (pnas.org)
  • Calcium binding proteins (CaBPs) such as calbindin D28-k, parvalbumin, and calretinin are able to bind Ca 2+ with high affinity. (hindawi.com)
  • To correlate the differential expression of AMPA receptor subunits in each neuron with that of two calcium-binding proteins, parvalbumin and calbindin-D28k, we used a triple-labeling method. (jneurosci.org)
  • Here, we have analyzed the expression of Scgn in dorsal root ganglia (DRGs) and trigeminal ganglia (TGs), and in spinal cord of mouse at the mRNA and protein levels, and in comparison to the well-known CaBPs, calbindin D-28k, parvalbumin and calretinin. (diva-portal.org)
  • This cytosolic protein belongs to a family of calcium-binding proteins that includes calmodulin, parvalbumin, troponin C, and S100 protein. (creativebiomart.net)
  • In order to increase the accuracy of tracer injections into target sites, we first conducted a systematic comparison of three calcium‐binding proteins, namely, parvalbumin, calretinin, and calbindin, to characterize the nucleus accumbens and ascertain its boundaries. (usf.edu)
  • It could act by binding to high affinity sites of the normal exocytosis machinery ( 5 ), by binding to special sites responsible for facilitation ( 2 , 6 ), or by modulating the degree of saturation of high affinity Ca 2+ buffers ( 7 , 8 ), but direct evidence in favor of any of these possibilities is still lacking. (pnas.org)
  • (EN) A fluorescent compound exhibiting a high affinity to calcium-binding proteins (CaBP) is provided. (wipo.int)
  • The canonical high affinity calcium binding site consists of the typical 12 CP-724714 amino acids of helix 3 (E) loop 2 and CP-724714 helix 4 (F) that has the shape of a CP-724714 human hand (EF-hand). (exposed-skin-care.net)
  • Cartilage Acidic Protein 2 a hyperthermostable, high affinity calcium-binding protein. (ualg.pt)
  • sbCRTAC2 bound Ca(2+) with high affinity (K(d)=1.46nM) and favourable Gibbs free energy (∆G=-12.4kcal/mol). (ualg.pt)
  • 1997). Fimbrin-mediated cross-linking and bundling of actin is regulated by free calcium, which it binds with high affinity and selectivity. (clicktocurecancer.info)
  • Alendronate-Functionalized Poly(2-oxazoline)s with Tunable Affinity for Calcium Cations. (bioportfolio.com)
  • We have isolated a group of atrial proteins by affinity chromatography that bind to atrial specific granules in a calcium-dependent manner. (ahajournals.org)
  • Intracellular storage and release of Ca2+ from the sarcoplasmic reticulum is associated with the high-capacity, low-affinity calcium-binding protein calsequestrin. (wikipedia.org)
  • binding site on the C-terminus and a low-affinity calcium mineral binding site on the N-terminus. (exposed-skin-care.net)
  • The non-canonical low affinity calcium binding EF-hand is definitely defined by 14 amino acids of helix 1 (E) loop 1 and helix 2 (F) (Number 1). (exposed-skin-care.net)
  • Membrane interactions during exocytosis are presumably mediated by the interaction of specific proteins with the granule membrane. (ahajournals.org)
  • The results suggest a role for vitamin D--dependent calcium binding protein in intracellular calcium metabolism rather than a direct involvement in membrane-mediated calcium reabsorption in the avian kidney. (sciencemag.org)
  • S100 proteins are a family of low-molecular weight (9-12 kD), calcium-binding proteins that initiate a number of cellular processes such as cell division, motility, secretion, protein synthesis, and membrane permeability through both calcium dependent and independent mechanism. (atlasgeneticsoncology.org)
  • Assembly of the sea urchin fertilization membrane: isolation of proteoliaisin, a calcium-dependent ovoperoxidase binding protein. (rupress.org)
  • Assembly of the fertilization membrane involves exocytosis of egg cortical granules, divalent cation-mediated association of exudate proteins with the egg glycocalyx (the vitelline layer), and cross-linking of the assembled structure by ovoperoxidase, a fertilization membrane component derived from the cortical granules. (rupress.org)
  • We have identified and isolated a new protein, which we call proteoliaisin, that appears to be responsible for inserting ovoperoxidase into the fertilization membrane. (rupress.org)
  • Binding is optimal over the physiological pH range of fertilization membrane assembly (pH 5.5-7.5). (rupress.org)
  • Translocation of STIM1 and STIM2 from the endoplasmic reticulum to the plasma membrane is a key step in store-operated calcium entry in the cell. (lu.se)
  • 3. Calcium-binding protein is synthesized on free rather than membrane-bound polyribosomes, which implies that it is an intracellular protein. (biochemj.org)
  • The protein may increaseCa2+absorption by buffering Ca2+ in the cytoplasm and increase ATP-dependent Ca2+ transport in duodenal basolateral membrane vesicles. (creativebiomart.net)
  • amate and a proton across the inner mitochondrial membrane, and is stimulated by calcium on the external side of the inner mitochondrial membrane. (mybiosource.com)
  • La photographie montre l'accumulation de p150 Glued à l'avant des cellules en migration, où la protéine pourrait participer à l'ancrage des microtubules à la membrane plasmique. (pasteur.fr)
  • The precise composition of the human sperm plasma membrane, the molecular interactions that define domain specific functions, and the regulation of membrane associated proteins during the capacitation process, still remain to be fully understood. (biomedcentral.com)
  • Here, we investigated the repertoire of calcium-regulated proteins associated with the human sperm plasma membrane. (biomedcentral.com)
  • The study of the sperm surface is complicated, however, by the organization of the plasma membrane into several distinctive domains, each with its own composition and function, by its complement of unique testis-specific proteins, which may be auto or iso-antigenic in males and females, and by the addition of secretory proteins originating in the male sex accessory glands. (biomedcentral.com)
  • Among physiologically important sperm surface molecules, the plasma membrane receptor(s) that mediates zona pellucida (ZP)-binding has not been unequivocally identified [ 1 , 2 ], and the receptor-induced signaling cascade that culminates in acrosomal exocytosis remains to be fully elucidated. (biomedcentral.com)
  • Signaling was associated with partitioning of RasGRP protein into the membrane fraction. (elsevier.com)
  • Sustained ligand-induced signaling and membrane partitioning were absent when the DAG-binding domain was deleted. (elsevier.com)
  • Membrane targeting of the EF-hand containing calcium-sensing proteins CaBP7 and CaBP8. (nih.gov)
  • Calretinin is another type of Calcium binding protein weighing 29kD. (wikipedia.org)
  • Compartmentalization of calcium binding proteins such as calretinin and calbindin-28 kDa has been noted within cells, suggesting that these proteins perform distinct functions in localized calcium signaling. (wikipedia.org)
  • Staining of calretinin, another calcium-binding protein, was intense in the rostral pole of the lateral BST, continuous with the caudal ventral striatum. (sinobiological.com)
  • This protein is 94 amino acids in length and 10.6 kilodaltons in mass. (biocompare.com)
  • All proteins are strings of amino acids that must fold into a unique shape to perform their function. (eurekalert.org)
  • There is a CP-724714 truncated form of murine S100A9 (amino acids 1-102) that is the result of protease activity and exhibits reduced zinc binding but this truncated peptide still retains the native disulfide bond formation between cysteine-79 and cysteine-90 [9]. (exposed-skin-care.net)
  • Although it is not yet known whether they play a 'triggering' role like calmodulin, or merely act as buffers to modulate cytosolic calcium transients, they are valuable markers of neuronal subpopulations for anatomical and developmental studies. (nih.gov)
  • Neuronal calcium-sensor proteins interact with other regulatory proteins to mediate physiological responses to a change in intracellular calcium concentration. (bioportfolio.com)
  • The related protein neuronal calcium sensor-1did not get affected on the [Ca2+] responses to agonist stimulation. (wikipedia.org)
  • Studies of the involvement of CacyBP/SIP in rearrangement of neuronal cytoskeleton, studies of the binding of CacyBP/SIP to tubulin and to other cytoskeletal proteins by means of chemical cross-linking, co-immunoprecipitation, immunocytochemistry etc. (gov.pl)
  • Ion-specific effects on peptides and proteins are key to biomolecular structure and stability. (bioportfolio.com)
  • Check out links to articles that cite our custom service antibodies, peptides, and proteins in major peer-reviewed journals, organized by research category. (abgent.com)
  • Calcium-binding proteins can be either intracellular and extracellular. (wikipedia.org)
  • Extracellular calcium-binding proteins are classified into six groups. (wikipedia.org)
  • The protein has been implicated in over 40 intra- and extracellular processes in mammalian cells, but the main research focus has been on its role in protein folding and as a regulator of cellular calcium homeostasis. (lu.se)
  • Calcium enhances Acanthamoeba polyphaga binding to extracellular matrix proteins. (arvojournals.org)
  • PURPOSE: To characterize better the ameba-host interactions that may be involved with the pathogenesis of Acanthamoeba keratitis, the role of calcium (Ca2+) on the binding of Acanthamoeba polyphaga to extracellular matrix proteins was examined in vitro. (arvojournals.org)
  • CONCLUSION: Low concentrations of calcium enhance the adhesion of A. polyphaga to extracellular matrix proteins. (arvojournals.org)
  • The progressive folding of Holo-RD in the extracellular calcium-rich environment as it emerges form the T1SS may then favor its unidirectional export through the secretory channel. (pasteur.fr)
  • The CCBE1 gene encodes a protein which is involved in extracellular matrix regulation and functions as a tumor suppressor. (moldiag.com)
  • With their role in signal transduction, calcium-binding proteins contribute to all aspects of the cell's functioning, from homeostasis to learning and memory. (wikipedia.org)
  • Calcium binding proteins are an important component of calcium mediated cellular signal transduction. (wikipedia.org)
  • Calcium-binding proteins play an important role in a variety of vital biological processes, ranging from blood clotting and signal transduction in cells, to attaching proteins to membranes and serving as an integral source of calcium. (springer.com)
  • Other names also exist, such as: protein S100-B, S-100 calcium-binding protein, beta chain, S-100 protein subunit beta, S100 calcium-binding protein, beta (neural). (biocompare.com)
  • Binds two calcium ions per subunit. (abcam.com)
  • In contrast, the receptor lacking this subunit exhibits a strong inward rectification and a high calcium permeability. (jneurosci.org)
  • The expression of S100A calcium-binding protein genes was compared with β-amyloid precursor protein gene, which had minimal variation in all normal and neoplastic gastric samples that we tested. (aacrjournals.org)
  • P53 is known as a tumor suppressor protein and in this case acts as a transcriptional activator or repressor of numerous genes. (wikipedia.org)
  • A potential role for S100 proteins in cancer originated from observations that the evolutionarily conserved gene cluster containing S100 genes on human chromosome 1q21 underwent several rearrangements during tumour development. (atlasgeneticsoncology.org)
  • Discover related pathways, diseases and genes to S100 calcium binding protein A14. (novusbio.com)
  • We also study the transcriptional and epigenetic regulation of genes encoding S100A6, Sgt1 and CacyBP/SIP, as well as posttranslational modifications of these proteins in relation to their function. (gov.pl)
  • A family of intracellular calcium-sensing proteins found predominately in NEURONS and PHOTORECEPTOR CELLS. (bioportfolio.com)
  • For example, the neuron-specific calexcitin has been found to have an excitatory effect on neurons, and interacts with proteins that control the firing state of neurons, such as the voltage-dependent potassium channel. (wikipedia.org)
  • Whether neurons within other visual regions also differentially express calcium-binding proteins, however, has not been extensively studied. (cambridge.org)
  • The cerebellar and IO projections originate from different neurons within the nBOR, but whether they can also be differentiated based on calcium-binding protein expression is unknown. (cambridge.org)
  • These results indicate that neurons in the rat somatosensory cortex express differential combinations of GluR subunits, which correlate with the specific expression of the calcium-binding proteins. (jneurosci.org)
  • Native AMPA receptors in the majority of CNS neurons display little calcium permeability. (jneurosci.org)
  • In contrast, there is evidence that neurons containing calcium-binding proteins exhibit a high resistance under experimental seizure and hypoxia conditions. (eurekamag.com)
  • The aim of this study was to determine the coexistence of NPY and calcium-binding proteins in inhibitory neurons of the primate fascia dentata and their synaptic connections. (eurekamag.com)
  • These results suggest that most NPY-immunoreactive neurons do not contain calcium-binding proteins. (eurekamag.com)
  • Thus, we conclude that the vulnerability of NPY-containing inhibitory neurons may be due more to the lack of calcium-binding proteins than to a strong excitatory innervation. (eurekamag.com)
  • Background: Secretagogin (Scgn), a member of the EF-hand calcium-binding protein (CaBP) superfamily, has recently been found in subsets of developing and adult neurons. (diva-portal.org)
  • Search, Find and Buy Antibodies, ELISA Kits and Proteins. (antibodies-online.com)
  • 16 S100 Calcium Binding Protein P (S100P) ELISA Kits from 7 manufacturers are available on www.antibodies-online.com. (antibodies-online.com)
  • We also selective antibodies against the expressed calcyclin and demonstrated that this protein is dominantly distributed in the cytoplasm by immunohistochemical and immunoblotting approaches. (nii.ac.jp)
  • Custom ELISA Kits, Recombinant Proteins and Antibodies can be designed, manufactured and produced according to the researcher's specifications. (mybiosource.com)
  • GENTAUR suppliers human normal cells, cell lines, RNA extracts and lots of antibodies and ELISA kits to Human proteins as well as Recombinant Human S100 Calcium Binding Protein A6 RECOMBINANT & NATURAL PROTEINS. (antibody-antibodies.com)
  • Intracellular calcium ion plays a critical role in cell activation in response to various agonists and calmodulin is a major intracellular receptor for calcium ion. (springer.com)
  • 5. These results suggest that calcium-binding protein is an intracellular protein, and that its synthesis may be a consequence of the raised intracellular calcium content of the intestinal epithelial cells resulting from 1,25-dihydroxycholecalciferol-stimulated Ca 2+ transport. (biochemj.org)
  • Fluctuations in intracellular calcium (Ca2+) are central to orderly neurotransmission and the operation of a wide range of cellular functions. (sinobiological.com)
  • Effect of divalent cations on bovine serum albumin (BSA) and tannic acid interaction and its influence on turbidity and in vitro protein digestibility. (bioportfolio.com)
  • The calcium dependence of their binding to atrial specific granules has been characterized in vitro and indicates that this interaction takes place at micromolar levels of calcium. (ahajournals.org)
  • In Calcium-Binding Protocols-Volume 1: Reviews and Case Studies and Volume 2: Methods and Techniques-Hans Vogel and a panel of leading researchers review the protein chemistry and behavior of this significant protein class, and provide a comprehensive collection of proven experimental techniques for their study both in vitro and in vivo. (springer.com)
  • 3. Studies in vitro suggested that the fall in ionized calcium and rise in protein-bound calcium were larger than could be explained by the redistribution of calcium fractions due to the pH change. (portlandpress.com)
  • In addition, GF14 omega is phosphorylated by Arabidopsis protein kinase activity at a serine residue(s) in vitro. (plantcell.org)
  • Western blot analysis showed increased phosphorylation of human sperm 80K-H protein following in vitro capacitation. (biomedcentral.com)
  • abstract = "RasGRP, a guanyl nucleotide-releasing protein for the small guanosine triphosphatase Ras, was characterized. (elsevier.com)
  • Using tools from the FEniCS library and the APBS solver, we implement a numerical code for our method and study two Ca2+-binding proteins: troponin C and the sarcoplasmic reticulum Ca2+ ATPase. (simula.no)
  • If you cannot find the target and/or product is not available in our catalog, please click here to contact us and request the product or submit your request for custom elisa kit production , custom recombinant protein production or custom antibody production . (mybiosource.com)
  • MBS725160 is a ready-to-use microwell, strip plate Competitive or Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the S100 Calcium Binding Protein A6 (S100A6) ELISA Kit target analytes in biological samples. (mybiosource.com)
  • Announcement: Third European Symposium on Calcium Binding Proteins in Normal and Transformed Cells," Metal-Based Drugs , vol. 1, no. 1, pp. 79-80, 1994. (hindawi.com)
  • Our approach handles molecular geometries generated from high-resolution structural data and can account for active sites buried within the protein or behind gating mechanisms. (simula.no)
  • In 1987 it was found in infiltrating macrophages of rheumatoid arthritis patients and named MRP-14 (myeloid related protein of molecular excess weight 14 kD) [12]. (exposed-skin-care.net)
  • Additional investigators have called it migration inhibitory element related protein (MRP) of molecular excess weight 14 kD due to its ability to translocate to keratin intermediate filaments in response to calcium activation [13]. (exposed-skin-care.net)
  • SELDI mass spectra showed that a protein with the molecular weight of 10.8 kDa was found to be elevated in tears from eyes with pterygium. (arvojournals.org)
  • With state-of-the art molecular biology and protein biochemistry labs, we work with our clients to rapidly evaluate in parallel to identify the optimal expression system for candidate proteins. (abgent.com)
  • Detailed case studies provide a wealth of valuable information about protein purification and characterization strategies, X-ray crystallography, and specific calcium-binding proteins and their modes of action. (springer.com)
  • Apart from one whole day focused on the fascinating roles played by calmodulin in cellular activities, the other sessions were devoted to calmodulin-related calcium binding proteins in muscle and non- muscle tissues and to some selected biological systems such as mitochondria, secretory cells or sarcoplasmic reticulum in which calcium also plays a crucial role. (springer.com)
  • Cation diffusion facilitator (CDF) proteins are a conserved family of transmembrane transporters that ensure cellular homeostasis of divalent transition metal cations. (bioportfolio.com)
  • Calcium-binding proteins are proteins that participate in calcium cell signalling pathways by binding to Ca2+, the calcium ion that plays an important role in many cellular processes. (wikipedia.org)
  • The cellular regulation of calcium is known as calcium homeostasis. (wikipedia.org)
  • Many different calcium-binding proteins exist, with different cellular and tissue distribution and involvement in specific functions. (wikipedia.org)
  • It also indicates that in addition to freely diffusing through the cytoplasm to attain a homogeneous distribution, calcium binding proteins can bind to cellular structures through interactions that are likely important for their functions. (wikipedia.org)
  • Our previous report showed that foot-and-mouth disease virus (FMDV) VP1 inhibited TNF-α- and SeV-induced type I interferon response via interaction with cellular protein soluble resistance-related calcium-binding protein (sorcin). (frontiersin.org)
  • Our recent publication shows that VP1 inhibits TNF-α-induced activation of NF-κB and type I interferon promoters via interaction with cellular protein soluble resistance-related calcium-binding protein (sorcin) ( 3 ). (frontiersin.org)
  • S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. (creative-biogene.com)
  • Furthermore, the inhibition of S100A1 and the overexpression of the serine/threonine‑protein kinase, large tumor suppressor homolog 1 (LATS1), inhibited the FOXD2‑AS1‑induced cellular proliferation, migration and invasiveness in breast cancer. (spandidos-publications.com)
  • At later times after the hormone, however, when Ca 2+ transport has declined to its basal rate, the cellular content of calcium-binding protein remains elevated. (biochemj.org)
  • S100A6: May function as calcium sensor and contribute to cellular calcium signaling (Potential). (mybiosource.com)
  • H. Hidaka, T. Yamaki, M. Naka, T. Tanaka, H. Haigawara, and R. Kobayashi, Calcium-regulated modulator protein interacting agents inhibit muscle calcium-stimulated protein kinase and ATPase. (springer.com)
  • it is a member of the 14-3-3 kinase regulatory protein family that is conserved throughout eukaryotes. (plantcell.org)
  • This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. (creative-biogene.com)
  • Furthermore, observation of the intracellular movement of secretory granules in the living pancreatic B-cell under a phase-contrast microscope revealed that myosin light chain kinase and CaM kinase II,two calmodulin-dependent protein kinases, are involved in the control of the insulin granules via phosphorylating their common substrate, myosin regulatory light chain. (nii.ac.jp)
  • HSPA2 (HSP70-1), HSPA5 (Bip), HYOU1 (ORP150), serum amyloid P-component (SAP) and protein kinase C substrate 80K-H (80K-H) were found to be accessible to Iodo-Bead catalyzed 125I-labelling on the surface of intact human sperm. (biomedcentral.com)
  • CAB39 protein and STE20-related adaptor-alpha pseudo kinase, form a regulatory complex that is able of stimulating the activity of the LKB1 tumor suppressor protein kinase. (neobiolab.com)
  • The binding is calcium dependent and the interaction correlates with calcium concentration. (wikipedia.org)
  • This interaction occurs in calcium dependent manner after the Synaptic NMDA receptor stimulation and thus this prevents nuclear import of NSMF. (wikipedia.org)
  • Calcium binding induces a conformational change in S100B that allows the interaction with a variety of target proteins. (atlasgeneticsoncology.org)
  • These compounds selectively inhibit the process of interaction of the calcium-calmodulin complex with target proteins. (springer.com)
  • Other divalent cations are less effective (Ba2+, Mn2+, and Sr2+) or ineffective (Mg2+ and Cd2+) in mediating the binding interaction. (rupress.org)
  • Preliminary characterization of this interaction indicated that the region in p53 involved with binding to S100A2 is located at the C terminus of p53. (uzh.ch)
  • Cytolytic T lymphocytes (CTL), natural killer cells, and lymphokine-activated killer (LAK) cells are cytolytic cells known to release the cytolytic protein perforin and a family of proteases, named granzymes, from cytoplasmic stores upon interaction with target cells. (unige.ch)
  • S100 proteins are found in the cytoplasm as preassembled dimers. (atlasgeneticsoncology.org)
  • Here, we report for the first time that during recovery from hydroxyurea treatment, the S100A2 protein translocated from the cytoplasm to the nucleus and co-localized with the tumor suppressor p53 in two different oral carcinoma cells (FADU and SCC-25). (uzh.ch)
  • Quantification of mRNA expression of S100A calcium-binding proteins S100A2, S100A7, S100A8, S100A9, and S100A10 was performed on 20 GC samples using iCycler (Biol-Rad). (aacrjournals.org)
  • We have studied the regulation, by 1,25-dihydroxyvitamin D 3 (1,25-(OH) 2 D 3 ), of vitamin D-dependent calcium-binding protein (28-kDa CaBP) mRNA in chick tissues in vivo. (elsevier.com)
  • It binds and transports cations across membranes and uncouples oxidative phosphorylation while inhibiting ATPase of rat liver mitochondria. (bioportfolio.com)
  • T. C. Theoharides, W. Sieghart, P. Greengard, and T. C. Douglas, Antiallergic drug cromolyn may inhibit histamine secretion by regulating phosphorylation of a mast cell protein. (springer.com)
  • Activation of ligand-gated channels, G-protein-coupled and growth factor receptors in OP cells causes CREB phosphorylation with a different temporal pattern. (jneurosci.org)
  • Phosphorylation and calcium binding properties of an Arabidopsis GF14 brain protein homolog. (plantcell.org)
  • calmodulin and cvclic 3, 5' adenosine monophosphate control movement of secretory granules through protein phosphorylation/dephosphorylation in the pancreatic β-cell. (nii.ac.jp)
  • Two calcium ions are bound in the Calbindin D9K version. (creativebiomart.net)
  • The protein consists of Calbindin D9K, mutant P47M (residues 5-79, swissprot accession P02633). (creativebiomart.net)
  • This gene encodes calbindin D9K, a vitamin D-dependent calcium-binding protein. (creativebiomart.net)
  • These include p53 tumour suppressor, the microtubule-associated protein tau , the cytoskeletal protein tubulin (and its prokaryotic ancestor FtsZ), the scaffold protein IQGAP1, the intermediate filament protein glial fibrillary acidic protein (GFAP),the actin capping protein CapZ and the giant phosphoprotein AHNAK. (atlasgeneticsoncology.org)
  • Fimbrin as well as actin capping protein (CP) are required for proper assembly of these filaments in the yeast Saccharomyces cerevisiae. (clicktocurecancer.info)
  • S100A2 is a member of the S100 family of calcium-binding proteins . (atlasgeneticsoncology.org)
  • S100B belongs to the S100 family of calcium binding proteins, a highly homologous family. (atlasgeneticsoncology.org)
  • The S100 family of calcium-binding proteins regulates many aspects of cell function but their roles in vascular physiology are less well understood. (sigmaaldrich.com)
  • S100A9 is now considered to be a member of the S100 family of calcium binding proteins [16]. (exposed-skin-care.net)
  • The heterodimer is definitely identified by the E210 antibody [6 7 S100A8/A9 heterodimerization is not dependent on calcium but formation of heterotetramers is definitely calcium dependent. (exposed-skin-care.net)
  • This protein is encoded by the gene S100A1. (biocompare.com)
  • 10.5 kDa monomer (S100B can form homodimers and heterodimers with other proteins of the S100 family, described for S100A1). (atlasgeneticsoncology.org)
  • S100 calcium binding protein A1 (S100A1) was also upregulated in breast cancer cell lines and was positively regulated by FOXD2‑AS1. (spandidos-publications.com)
  • Annexins V and VI: major calcium-dependent atrial secretory granule-binding proteins. (ahajournals.org)
  • We have obtained new findings on the roles of calcium-binding proteins in the secretory events as described below. (nii.ac.jp)
  • One of the functions of calcium binding proteins is to regulate the amount of free (unbound) Ca2+ in the cytosol of the cell. (wikipedia.org)
  • due: Historical perspective of calcium binding protein The historical understanding of the application of calcium in biology forms the basis understanding of the core functions of calcium binding proteins. (lightingresearch.org)
  • Combining nuclear magnetic resonance (NMR) spectroscopy and other biochemical methods, researchers also found that the normal copy of this fragment was unable to fold properly in the absence of calcium, and adding calcium restored its folding ability. (eurekalert.org)
  • Based on these data and other biochemical information, researchers speculate that the mutations in the LDL receptor affect the receptor1s ability to bind calcium and therefore its ability to fold into its proper shape. (eurekalert.org)
  • Therefore, GF14 omega protein has biochemical properties consistent with potential signaling roles in plants. (plantcell.org)
  • This suggests biochemical practical equivalence of the human and the murine proteins despite a relatively low degree of sequence homology (59%) [10]. (exposed-skin-care.net)
  • S100Z has several biochemical functions, for example, calcium ion binding. (creativebiomart.net)
  • In addition, this explains why this protein can easily interact with p53 when transcriptional regulation takes place. (wikipedia.org)
  • Although a similar Ca2+ binding capacity is evident for plant calreticulins, the physiological relevance of the protein in endoplasmic reticulum Ca2+ regulation has been elusive. (lu.se)
  • cMyBP-C, an accessory protein that binds tightly to myosin, has an important role in thick filament regulation. (ahajournals.org)
  • Since 80K-H is a Ca2+-sensor recently implicated in the regulation of both inositol 1,4,5-trisphosphate receptor and transient receptor potential (TRP) cation channel activities, its detection in sperm represents the first direct signaling link between PKC and store-operated calcium channels identified in human sperm. (biomedcentral.com)
  • M, produced confor-mational changes in the molecule, to which they attribute the calcium-mediated regulation of fimbrin function. (clicktocurecancer.info)
  • Subtractive hybridization of tumorigenic versus normal tumor-derived mammary epithelial cells has previously identified the S100A2 protein as potential tumor suppressor. (uzh.ch)
  • Also, another member of the EF-hand superfamily is the S100B protein, which regulates p53. (wikipedia.org)
  • The protein encoded by this gene regulates the gating of voltage-gated calcium ion channels. (wikipedia.org)
  • This protein also regulates calcium-dependent activity of inositol 1,4,5-triphosphate receptors, P/Q-type voltage-gated calcium channels, and transient receptor potential channel TRPC5. (wikipedia.org)
  • This protein is found in all eukaryotic cells, where it regulates and modifies the activities of many calcium-binding enzymes. (sciencephoto.com)
  • The S100A6 calcium-binding protein regulates endothelial cell-cycle progression and senescence. (sigmaaldrich.com)
  • This gene encodes a protein that belongs to a subfamily of calcium binding proteins which share similarity to calmodulin. (wikipedia.org)
  • This gene encodes a protein with high similarity to the calcium-binding proteins of the calmodulin family. (nih.gov)
  • Lines of evidence showed that negative regulating proteins played an essential role in homeostasis, such as suppressors of SOCS proteins as counterparts for STAT ( 4 , 5 ), Mdm2 as negative regulator for p53 ( 6 - 8 ), and CTLA4 as a brake for TCR-mediated T cell activation ( 9 - 11 ). (frontiersin.org)
  • Calcium-binding proteins have a vital role in calcium homeostasis by buffering and probably also have a neuroprotective function. (sinobiological.com)
  • S100A9 is a calcium binding protein with multiple ligands and post-translation modifications that's involved with inflammatory events and the original advancement of the cancer cell to the introduction of metastatic disease. (exposed-skin-care.net)
  • Number 1 Ribbon diagram of homodimeric calcium bound S100A9 from protein data bank file 1IRJ using the program Chimera. (exposed-skin-care.net)
  • The three dimensional constructions of the calcium bound S100A9 homodimer S00A8/A9 heterodimer and heterotetramer of S100A8/A9 are known [2-4]. (exposed-skin-care.net)
  • In the heterodimer the C-terminus of S100A9 and the N-terminus of S100A8 are aligned in an anti-parallel fashion similar to additional homodimeric S100 proteins. (exposed-skin-care.net)
  • Based on this data and structural data listed above the zinc binding site is definitely proposed to be located on the C-terminal region near a series of histidine residues but a Zn2+-S100A9 structure has not been determined to day. (exposed-skin-care.net)
  • S100A9 appears to be specific in its dimerization partners as S100A12 another S100 protein CP-724714 involved in swelling does not dimerize with S100A9 [11]. (exposed-skin-care.net)
  • The large quantity of p14 (synonym for S100A9) in neutrophils and monocytes was confirmed in 1991 by Edgeworth and this was followed by the 1st large level purification of the protein for structure dedication [14]. (exposed-skin-care.net)
  • 16] Calprotectin = S100A8/A9 Calcium mineral sure S100A9 binds to arachidonic acidity cytoskeletal elements (e.g. keratin intermediate filaments) Receptor for CP-724714 Advanced Glycation Endproducts (Trend) Toll-Like Receptor 4 (TLR4) the main fatty acidity transporter Compact disc36 matrix metallo-proteinases (MMPs) fibronectin and heparin sulfate. (exposed-skin-care.net)
  • Calcium binding proteins also serve an important physiological role for cells. (wikipedia.org)
  • Although a similar Ca2+ binding capacity is evident for plant calreticulins, the physiological relevance of the. (lu.se)
  • Equilibrium dialysis further demonstrated that GF14 omega binds an equimolar amount of calcium with an apparent binding constant of 5.5 x 10(4) M-1 under physiological conditions. (plantcell.org)
  • Upon binding to calcium there is a conformational switch whereby helix 3 rotates therefore exposing a hydrophobic cleft that is postulated to serve as an anchoring point for macromolecular relationships (Number 1) [1]. (exposed-skin-care.net)
  • It may be that the conformational alterations actuated by calcium at different concentrations could render actin cross-linking reversible. (clicktocurecancer.info)
  • Coordination to Divalent Cations by Calcium-Binding Proteins. (bioportfolio.com)
  • Tannins, proteins, and divalent cations interactions are important for many processes in the food industry and human and animal nutrition and health. (bioportfolio.com)
  • Binding to collagen IV was studied in detail, with and without other divalent cations and calcium channel modulators. (arvojournals.org)
  • Molecule model of the calcium-binding protein calmodulin (CaM). (sciencephoto.com)
  • Then, the target protein becomes active and transfer calcium signal to the next molecule. (springer.com)
  • The stoichiometry for Ca(2+) bound to sbCRTAC2 at saturation indicated six Ca(2+) ligand-binding sites exist per protein molecule. (ualg.pt)
  • In actin filaments, one molecule of fimbrin might bind eight actin monomers under optimal conditions (Namba et al. (clicktocurecancer.info)
  • CABP1 also interacts with CACNA1A in the pre and post synaptic membranes via C-terminal calcium binding motif. (wikipedia.org)
  • The S100A2 gene has a total length of 4723 bp and seven splice variants, which can be categorized into five variants with protein product and two transcripts without an open-reading frame. (atlasgeneticsoncology.org)
  • The protein structure and amino acid sequence of S100A2 (human). (atlasgeneticsoncology.org)
  • Head and neck squamous cell carcinoma express high levels of the EF-hand calcium-binding protein S100A2 in contrast to other tumorigenic tissues and cell lines where the expression of this protein is reduced. (uzh.ch)
  • Senescence Marker Protein (SMP30) is a metalloenzyme that shows lactonase activity in the ascorbic acid (AA) biosynthesis pathway in non-primate mammals such as a mouse. (bioportfolio.com)
  • This is at least 2h before calcium-binding protein can be detected immunologically and 1h before synthesis of the protein begins on polyribosomes, and thus the hormone stimulates Ca 2+ transport before calcium-binding-protein biosynthesis is induced. (biochemj.org)
  • Calcium-binding protein expression has proven useful in delineating neural pathways. (cambridge.org)
  • Greater than twenty five different S100 proteins have so far been identified with many mapping closely together on chromosome 1q21. (atlasgeneticsoncology.org)
  • In humans, this protein has a Uniprot ID of P04271 and has an annotated function: 'Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. (biocompare.com)
  • The protein comprises three distinct regions: the N-terminal N domain, the C-terminal C domain, and the central P domain, reflecting the functional diversity of calreticulin. (lu.se)
  • The properties of the proteins described here, and their ability to bind to atrial specific granules in a calcium-dependent manner, make them likely candidates in the search for regulatory proteins mediating atrial natriuretic peptide secretion. (ahajournals.org)
  • Calcyclin, was another calcium-binding proteins found to participate in the control of insulin secretion by ourselves. (nii.ac.jp)
  • In the low calcium environment of the bacterial cytosol, Apo-RD is an elongated disordered coil appropriately sized for transport through the narrow secretion machinery. (pasteur.fr)
  • They can act as transport proteins, regulator proteins, or activator proteins. (curehunter.com)
  • J. M. Marcum, J. R. Dedman, B. R. Brinkley, and A. R. Means, Control of microtubule assembly-disassembly by calcium-dependent regulator protein. (springer.com)
  • Solution NMR structure of the Ca2+-bound N-terminal domain of CaBP7: a regulator of golgi trafficking. (nih.gov)
  • View conserved domains detected in this protein sequence using CD-search. (nih.gov)
  • See the reference protein sequence for PREDICTED: polcalcin Bra r 1 (XP_009126190.1). (nih.gov)
  • See 2 reference sequence protein isoforms for the ANXA1 gene. (nih.gov)
  • Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed. (uniprot.org)
  • p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence. (uniprot.org)
  • Calreticulin is a calcium storage protein and carries a COOH-terminal KDEL sequence, known to act as a retention signal for proteins destined to the lumen of the endoplasmic reticulum. (unige.ch)
  • S100A6, a calcium - and zinc- binding protein, is overexpressed in SOD1 mutant mice, a model for amyotrophic lateral sclerosis. (wroc.pl)
  • The primary objective is to determine whether meat interacts positively with calcium to improve calcium retention. (bioportfolio.com)
  • Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest. (uniprot.org)
  • Analysis of total endothelial mRNAs using a human gene chip array revealed significant gene expression of the S100 calcium-binding protein family members S100A6, S100A10, S100A11 and S100A13. (sigmaaldrich.com)
  • The S100A13-encoded protein plays a role in the export of proteins that lack a signal peptide, which are secreted by an alternative pathway. (thefreedictionary.com)
  • The most ubiquitous Ca2+-sensing protein, found in all eukaryotic organisms including yeasts, is calmodulin. (wikipedia.org)
  • The inhibitory effects of brain calmodulin and S-100 were overcome by the addition of Tetrahymena calmodulin, but not by calcium. (nih.gov)
  • Since the roles of calcyclin will be better understood if we can visualize the localization of this calcyclin-binding protein in a living cell, we are now attempting to let insulinoma cells express the fusion protein between calcyclin and green fluorescent protein as a fluorescent marker. (nii.ac.jp)
  • Antiserum directed against calcium-binding protein isolated from human kidneys was used for the immunofluorescent localization of calcium-binding protein in human intestine and kidney. (bmj.com)
  • Calcium-binding protein 1 (CaBP1) is placed in the lumen of the endoplasmic reticulum.it is relocated outside cells during apoptosis and involved in the phagocytosis of apoptotic cells. (wikipedia.org)
  • This type of protein is also found in large quantities in malignant mesothelial cells, which can be easily differentiated from carcinomas. (wikipedia.org)
  • S100B proteins are abundantly found in cancerous tumor cells causing them to be overexpressed, therefore making these proteins useful for classifying tumors. (wikipedia.org)
  • In brain, CaBp1 is found in the cerebral cortex and hippocampus and in the protein, Cabp1 is found in cone bipolar and amacrine cells. (wikipedia.org)
  • The intercalated (mitochondria-rich) cells in these tubular segments were negative for the calcium binding protein. (sciencemag.org)
  • Subcellularly, the protein was found in the cytosol and the nucleus of the tubular cells. (sciencemag.org)
  • Comparison of primary and transformed human cells revealed significant differences in S100A6 protein levels in these cells. (sigmaaldrich.com)
  • Specific fluorescence indicating the presence of calciumbinding protein was observed at both the basal and apical poles of the intestinal absorptive cells while the goblet cells appeared to fluoresce non-specifically. (bmj.com)
  • Moreover, the calcium bioavailability in Caco-2 cells showed that FY-Ca could enhance calcium uptake efficiency by more than three times when compared with CaCl2, and protect calcium ions against dietary inhibitors, such as tannic acid, oxalate, phytate, and Zn2+. (bionity.com)
  • Abgent has over fifteen years of experience producing recombinant proteins in E. coli and mammalian cells (CHO and HEK293, etc), and we have added a powerful yeast expression platform to our menu of services. (abgent.com)
  • We now report the purification of an additional major 60-kD granule-associated protein (grp 60) from human LAK cells and from mouse cytolytic T cells. (unige.ch)
  • Studies of the role of S100A6 and CacyBP/SIP in protein ubiquitination using cells overexpressing or deficient in S100A6 and CacyBP/SIP due to transfection with appropriate plasmids. (gov.pl)