Calcium-binding proteins that are found in DISTAL KIDNEY TUBULES, INTESTINES, BRAIN, and other tissues where they bind, buffer and transport cytoplasmic calcium. Calbindins possess a variable number of EF-HAND MOTIFS which contain calcium-binding sites. Some isoforms are regulated by VITAMIN D.
A calbindin protein found in many mammalian tissues, including the UTERUS, PLACENTA, BONE, PITUITARY GLAND, and KIDNEYS. In intestinal ENTEROCYTES it mediates intracellular calcium transport from apical to basolateral membranes via calcium binding at two EF-HAND MOTIFS. Expression is regulated in some tissues by VITAMIN D.
A calcium-binding protein that mediates calcium HOMEOSTASIS in KIDNEYS, BRAIN, and other tissues. It is found in well-defined populations of NEURONS and is involved in CALCIUM SIGNALING and NEURONAL PLASTICITY. It is regulated in some tissues by VITAMIN D.
A calbindin protein that is differentially expressed in distinct populations of NEURONS throughout the vertebrate and invertebrate NERVOUS SYSTEM, and modulates intrinsic neuronal excitability and influences LONG-TERM POTENTIATION. It is also found in LUNG, TESTIS, OVARY, KIDNEY, and BREAST, and is expressed in many tumor types found in these tissues. It is often used as an immunohistochemical marker for MESOTHELIOMA.
Low molecular weight, calcium binding muscle proteins. Their physiological function is possibly related to the contractile process.
The modification of the reactivity of ENZYMES by the binding of effectors to sites (ALLOSTERIC SITES) on the enzymes other than the substrate BINDING SITES.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS.
The shortest and widest portion of the SMALL INTESTINE adjacent to the PYLORUS of the STOMACH. It is named for having the length equal to about the width of 12 fingers.
A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.
Agents, usually topical, that cause the contraction of tissues for the control of bleeding or secretions.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
The homogeneous mixtures formed by the mixing of a solid, liquid, or gaseous substance (solute) with a liquid (the solvent), from which the dissolved substances can be recovered by physical processes. (From Grant & Hackh's Chemical Dictionary, 5th ed)
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
NMR spectroscopy on small- to medium-size biological macromolecules. This is often used for structural investigation of proteins and nucleic acids, and often involves more than one isotope.
An order of BIRDS with the common name owls characterized by strongly hooked beaks, sharp talons, large heads, forward facing eyes, and facial disks. While considered nocturnal RAPTORS, some owls do hunt by day.
Proteins found mainly in icosahedral DNA and RNA viruses. They consist of proteins directly associated with the nucleic acid inside the NUCLEOCAPSID.
The practice of medicine as applied to special circumstances associated with military operations.
Connective tissue cells of an organ found in the loose connective tissue. These are most often associated with the uterine mucosa and the ovary as well as the hematopoietic system and elsewhere.
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
Bone-forming cells which secrete an EXTRACELLULAR MATRIX. HYDROXYAPATITE crystals are then deposited into the matrix to form bone.
The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.
The female reproductive organs. The external organs include the VULVA; BARTHOLIN'S GLANDS; and CLITORIS. The internal organs include the VAGINA; UTERUS; OVARY; and FALLOPIAN TUBES.
Exogenous agents, synthetic and naturally occurring, which are capable of disrupting the functions of the ENDOCRINE SYSTEM including the maintenance of HOMEOSTASIS and the regulation of developmental processes. Endocrine disruptors are compounds that can mimic HORMONES, or enhance or block the binding of hormones to their receptors, or otherwise lead to activating or inhibiting the endocrine signaling pathways and hormone metabolism.
The hollow thick-walled muscular organ in the female PELVIS. It consists of the fundus (the body) which is the site of EMBRYO IMPLANTATION and FETAL DEVELOPMENT. Beyond the isthmus at the perineal end of fundus, is CERVIX UTERI (the neck) opening into VAGINA. Beyond the isthmi at the upper abdominal end of fundus, are the FALLOPIAN TUBES.
The system of glands that release their secretions (hormones) directly into the circulatory system. In addition to the ENDOCRINE GLANDS, included are the CHROMAFFIN SYSTEM and the NEUROSECRETORY SYSTEMS.

Energy-based de novo protein folding by conformational space annealing and an off-lattice united-residue force field: application to the 10-55 fragment of staphylococcal protein A and to apo calbindin D9K. (1/704)

The conformational space annealing (CSA) method for global optimization has been applied to the 10-55 fragment of the B-domain of staphylococcal protein A (protein A) and to a 75-residue protein, apo calbindin D9K (PDB ID code), by using the UNRES off-lattice united-residue force field. Although the potential was not calibrated with these two proteins, the native-like structures were found among the low-energy conformations, without the use of threading or secondary-structure predictions. This is because the CSA method can find many distinct families of low-energy conformations. Starting from random conformations, the CSA method found that there are two families of low-energy conformations for each of the two proteins, the native-like fold and its mirror image. The CSA method converged to the same low-energy folds in all cases studied, as opposed to other optimization methods. It appears that the CSA method with the UNRES force field, which is based on the thermodynamic hypothesis, can be used in prediction of protein structures in real time.  (+info)

Expression of calcium binding protein D-9k messenger RNA in the mouse uterine endometrium during implantation. (2/704)

To investigate the molecular mechanisms of implantation, we constructed a cDNA library of mouse uteri enriched with pregnancy-induced genes by subtractive hybridization and polymerase chain reaction (PCR). One of the isolated clones was the cDNA for the calcium binding protein D-9k (Cabp9k), which is considered to regulate intracytoplasmic concentration and transport of free calcium ions. Northern blot and in-situ hybridization analyses demonstrated that the Cabp9k mRNA was expressed in the endometrial epithelia, both luminal and glandular, in the uterus at the time of implantation. On pregnancy day 5 it was detected in the luminal, but not in the glandular, epithelia. In the oophorectomized adult mice, progesterone enhanced Cabp9k mRNA expression in the uterus, whereas oestrogen did not. Consistent with this, a nucleotide change was identified in the first intron of mouse Cabp9k gene corresponding to the oestrogen responsive element in the rat Cabp9k gene. Transfer of embryos into the uterine cavity of pseudopregnant mice reduced the expression of Cabp9k mRNA in the glandular epithelium, suggesting that Cabp9k mRNA expression is also regulated by embryonal signal(s). These findings demonstrated that Cabp9k mRNA is expressed in the endometrial epithelia during the implantation period under the control of progesterone and the presence of embryo, and suggest that CaBP9k plays a role in implantation by regulating the local calcium concentrations.  (+info)

Distribution of cholinergic contacts on Renshaw cells in the rat spinal cord: a light microscopic study. (3/704)

1. Cholinergic terminals in the rat spinal cord were revealed by immunohistochemical detection of the vesicular acetycholine transporter (VAChT). In order to determine the relationships of these terminals to Renshaw cells, we used dual immunolabelling with antibodies against gephyrin or calbindin D28k to provide immunohistochemical identification of Renshaw cells in lamina VII of the ventral horn. 2. A total of 50 Renshaw cells were analysed quantitatively using a computer-aided reconstruction system to provide accurate localization of contact sites and determination of somatic and dendritic surface area. Dendrites could be traced for up to 413 microm from the soma in calbindin D28k-identified Renshaw cells and up to 184 microm in gephyrin-identified cells. 3. A total of 3330 cholinergic terminals were observed on 50 Renshaw cells, with a range of 21-138 terminal appositions per cell (mean 66.6 +/- 25.56 contacts per cell). The vast majority (83.5 %) of the terminals were apposed to dendrites rather than the soma. The overall density of cholinergic contacts increased from a little above 1 per 100 microm2 on the soma and initial 25 microm of proximal dendrites to 4-5 per 100 microm2 on the surface of dendritic segments located 50-250 microm from the soma. Single presynaptic fibres frequently formed multiple contacts with the soma and/or dendrites of individual Renshaw cells. 4. VAChT-immunoreactive terminals apposed to Renshaw cells varied in size from 0.6 to 6.9 microm in diameter (mean 2.26 +/- 0.94; n = 986) and were on average smaller than the cholinergic C-terminals apposed to motoneurones, but larger than VAChT-immunoreactive terminals contacting other ventral horn interneurones. 5. The high density and relatively large size of many cholinergic terminals on Renshaw cells presumably correlates with the strong synaptic connection between motoneurones and Renshaw cells. The fact that the majority of contacts are distributed over the dendrites makes the motoneurone axon collateral input susceptible to inhibition by the prominent glycinergic inhibitory synapses located on the soma and proximal dendrites. The relative positions and structural features of the excitatory cholinergic and inhibitory glycinergic synapses may explain why Renshaw cells, although capable of firing at very high frequency following motor axon stimulation, appear to fire at relatively low rates during locomotor activity.  (+info)

Molecular identification of the apical Ca2+ channel in 1, 25-dihydroxyvitamin D3-responsive epithelia. (4/704)

In mammals, the extracellular calcium concentration is maintained within a narrow range despite large variations in daily dietary input and body demand. The small intestine and kidney constitute the influx pathways into the extracellular Ca2+ pool and, therefore, play a primary role in Ca2+ homeostasis. We identified an apical Ca2+ influx channel, which is expressed in proximal small intestine, the distal part of the nephron and placenta. This novel epithelial Ca2+ channel (ECaC) of 730 amino acids contains six putative membrane-spanning domains with an additional hydrophobic stretch predicted to be the pore region. ECaC resembles the recently cloned capsaicin receptor and the transient receptor potential-related ion channels with respect to its predicted topology but shares less than 30% sequence homology with these channels. In kidney, ECaC is abundantly present in the apical membrane of Ca2+ transporting cells and colocalizes with 1,25-dihydroxyvitamin D3-dependent calbindin-D28K. ECaC expression in Xenopus oocytes confers Ca2+ influx with properties identical to those observed in distal renal cells. Thus, ECaC has the expected properties for being the gatekeeper of 1,25-dihydroxyvitamin D3-dependent active transepithelial Ca2+ transport.  (+info)

Immunohistological studies of metabotropic glutamate receptor subtype 6-deficient mice show no abnormality of retinal cell organization and ganglion cell maturation. (5/704)

Immature retinal ganglion cells (RGCs) initially show a multistratified dendritic pattern, and, during the postnatal period, these dendrites gradually monostratify into ON and OFF sublaminae. The selective agonist of group III metabotropic glutamate receptors (mGluR), L-2-amino-4-phosphonobutyrate (L-AP-4), hyperpolarizes ON bipolar cells and reduces glutamate release. On the basis of L-AP-4-evoked inhibitory effects on ON-OFF segregation of developing RGCs, it has been hypothesized that glutamate-mediated synaptic activity is crucial for formation of the ON-OFF network. Gene-targeted ablation of mGluR6 specifically expressed in ON bipolar cells blocks normal ON responses but has been predicted to enhance glutamate release from ON bipolar cells. The mGluR6 knock-out mouse therefore provides a unique opportunity to investigate whether glutamate release and ON responses are important factors in the development of ON-OFF segregation. The combination of several different morphological analyses indicates that ON bipolar cells, as well as several distinct amacrine cells, in mGluR6 knock-out mice are normally distributed and correctly extend their terminals to defined retinal laminae. Importantly, both alpha and delta RGCs in adult mGluR6 knock-out mice are found monostratified into cell type-specific layers. Furthermore, no difference between wild-type and mGluR6 knock-out mice is observed in the maturation and dendritic stratification of developing RGCs. Hence, despite a deficit in normal ON responses, mGluR6 deficiency causes no abnormality in the retinal cellular organization nor in the stratifications of both ON bipolar cells and developing and mature RGCs. Based on these findings, we discuss several possible mechanisms that may underlie ON-OFF segregation of RGCs.  (+info)

Expression of type 2 iodothyronine deiodinase in hypothyroid rat brain indicates an important role of thyroid hormone in the development of specific primary sensory systems. (6/704)

Thyroid hormone is an important epigenetic factor in brain development, acting by modulating rates of gene expression. The active form of thyroid hormone, 3,5,3'-triiodothyronine (T3) is produced in part by the thyroid gland but also after 5'-deiodination of thyroxine (T4) in target tissues. In brain, approximately 80% of T3 is formed locally from T4 through the activity of the 5'-deiodinase type 2 (D2), an enzyme that is expressed mostly by glial cells, tanycytes in the third ventricle, and astrocytes throughout the brain. D2 activity is an important point of control of thyroid hormone action because it increases in situations of low T4, thus preserving brain T3 concentrations. In this work, we have studied the expression of D2 by quantitative in situ hybridization in hypothyroid animals during postnatal development. Our hypothesis was that those regions that are most dependent on thyroid hormone should present selective increases of D2 as a protection against hypothyroidism. D2 mRNA concentration was increased severalfold over normal levels in relay nuclei and cortical targets of the primary somatosensory and auditory pathways. The results suggest that these pathways are specifically protected against thyroid failure and that T3 has a role in the development of these structures. At the cellular level, expression was observed mainly in glial cells, although some interneurons of the cerebral cortex were also labeled. Therefore, the T3 target cells, mostly neurons, are dependent on local astrocytes for T3 supply.  (+info)

Specification of somatosensory area identity in cortical explants. (7/704)

The H-2Z1 transgene is restricted to a subset of layer IV neurons in the postnatal mouse cortex and delineates exactly the somatosensory area. Expression of the H-2Z1 transgene was used as an areal marker to determine when the parietal cortex becomes committed to a somatosensory identity. We have shown previously that grafts dissected from embryonic day 13.5 (E13.5) H-2Z1 cortex and transplanted into the cortex of nontransgenic newborns express H-2Z1 according to their site of origin. Expression was not modified on heterotopic transplantation (). In the present study, whole cortical explants were isolated at E12.5 from noncortical tissues. The explants developed a regionalized expression of H-2Z1, indicating that regionalization takes place and is maintained in vitro. We used this property and confronted embryonic H-2Z1 cortex with presumptive embryonic sources of regionalizing signals in an in vitro grafting procedure. A great majority of E11.5-E13.5 grafts maintained their presumptive expression of H-2Z1 when grafted heterotopically on nontransgenic E13.5-E15.5 explants. However, a significantly lower proportion of E11.5 parietal grafts expressed H-2Z1 in occipital compared with parietal cortex, indicating that somatosensory identity may be partially plastic at E11.5. Earlier stages could not be tested because the E10.5 grafts failed to develop in vitro. The data suggest that commitment to the expression of a somatosensory area-specific marker coincides with the onset of neurogenesis and occurs well before the birth of the non-GABAergic neurons that express H-2Z1 in vivo.  (+info)

Expression of the striatal DARPP-32/ARPP-21 phenotype in GABAergic neurons requires neurotrophins in vivo and in vitro. (8/704)

The medium spiny neuron (MSN) is the major output neuron of the caudate nucleus and uses GABA as its primary neurotransmitter. A majority of MSNs coexpress DARPP-32 and ARPP-21, two dopamine and cyclic AMP-regulated phosphoproteins, and most of the matrix neurons express calbindin. DARPP-32 is the most commonly used MSN marker, but previous attempts to express this gene in vitro have failed. In this study we found that DARPP-32 is expressed in <12% of E13- or E17-derived striatal neurons when they are grown in defined media at high or low density in serum, dopamine, or Neurobasal/N2 (Life Technologies), and ARPP-21 is expressed in <1%. The percentage increases to 25% for DARPP-32 and 10% for ARPP-21 when the same cells are grown in Neurobasal/B27 (Life Technologies) for 7 d. After growth in Neurobasal/B27 plus brain-derived neurotrophic factor (BDNF) for 7 d, E13-derived MSNs are 53.7% DARPP-32-positive and 29. 0% ARPP-21-positive; E17-derived MSNs are 66.8% DARPP-32-positive and 51.5% ARPP-21-positive. The percentage of calbindin-positive neurons also is increased under these conditions. Finally, ARPP-21 expression is reduced in mice with a targeted deletion of the BDNF gene. We conclude that BDNF is required for the maturation of a large subset of patch and matrix MSNs in vivo and in vitro. In addition, we introduce a culture system in which highly differentiated MSNs may be generated, maintained, and studied.  (+info)

Inhibitory GABAergic interneurons are important for shaping patterns of activity in neocortical networks. We examined the distributions of inhibitory interneuron subtypes in layer II/III of areas V1 and V2 in 18 genera of anthropoid primates including New World monkeys, Old World monkeys, and hominoids (apes and humans). Interneuron subtypes were identified by immunohistochemical staining for calbindin, calretinin, and parvalbumin and densities were quantified using the optical disector method. In both V1 and V2, calbindin-immunoreactive neuron density decreased disproportionately with decreasing total neuronal density. Thus, V1 and V2 of hominoids were occupied by a smaller percentage of calbindin-immunoreactive interneurons compared to monkeys who have greater overall neuronal densities. At the transition from V1 to V2 across all individuals, we found a tendency for increased percentages of calbindin-immunoreactive multipolar cells and calretinin-immunoreactive interneurons. In addition, ...
DAB staining of tissue with this antibody showed high specificity and very low background at a concentration of 1:2000. For fluorescence staining of tissue. the optimal concentration was 1:1000.. This was also tested on neurons in culture where a clear difference between Calbindin-positive and -negative was seen at a concentration of 1:1000.. This was not tested for IP or WB.. ...
Rabbit Polyclonal Anti-Calbindin D-28K Antibody - Pre-diluted. Validated: IHC, IHC-P. Tested Reactivity: Human, Mouse, Rat. 100% Guaranteed.
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Looking for online definition of Epithelial calcium channel 2 in the Medical Dictionary? Epithelial calcium channel 2 explanation free. What is Epithelial calcium channel 2? Meaning of Epithelial calcium channel 2 medical term. What does Epithelial calcium channel 2 mean?
In dieser Arbeit werden Struktur-Funktionsbeziehungen in der medialen entorhinalen Hirnrinde untersucht. Schicht 2 Neurone im medialen entorhinalen Cortex unterteilen sich in calbindin-positive Pyramidenzellen und calbindin-negative Sternzellen. Calbindin-positive Pyramidenzellen bündeln ihre apikalen Dendriten zusammen und formen Zellhaufen, die in einem hexagolen arrangiert sind. Das Gitter von calbindin-positiven Pyramidenzellhaufen ist an Schicht 1 Axonen und dem Parasubiculum ausgerichtet und wird durch cholinerge Eingänge innerviert. Calbindin-positive Pyramidenzellen zeigen stark theta-modulierte Aktivität. Sternzellen sind vertreut in der Schicht 2 angeordnet und zeigen nur schwach theta-modulierte Aktivität, ein Befund, der gegen eine Rolle von zell-intrinsischen Oszillationen in der Entstehung von Theta-Modulation spricht. In der Arbeit wurden Methoden entwickelt, um durch die juxtazelluläre Färbung und Identifikation von Zellen, die räumlichen Feuermuster von Schicht 2 Sternzellen
1BOC: The solution structures of mutant calbindin D9ks, as determined by NMR, show that the calcium-binding site can adopt different folds.
1IG5: Structural basis for the negative allostery between Ca(2+)- and Mg(2+)-binding in the intracellular Ca(2+)-receptor calbindin D9k.
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Calbindin je termín označující skupinu transportních proteinů v buňkách tenkého střeva a v ledvinách. Tyto proteiny jsou závislé na funkci vitaminu D a umožňují absorpci vápníku ze střeva a ledvinných tubulů do organizmu ...
the equilibrium constant for a solubility reaction. A general solubility equation could be written as: MX (s) M+ (aq) + X- (aq) where MX represents an ionic solid, M+ the positive ion, and X- the negative ion. Since pure solids have an activity of 1, they are not included in equilibrium constant expressions. Therefore, the K expression for this reaction is: Since the concentration of either ion, [M+] or [Cl-] is the amount of MX that dissolved, (in other words, MXs solubility) and the result of multiplying two numbers is called a product, this kind of K expression is called a solubility product, and given the symbol Ksp. Therefore the K expression would normally be written ...
Fast delivery of PPM1K knockout Human Cell Lines for the study of gene function. Created by CRISPR/Cas9 genome editing. Includes matched wildtype control.
Disclaimer: TRIP Database staffs have endeavored to ensure the scientific accuracy of all entries to the database, but cannot take any responsibility for errors ...
DI-fusion, le Dépôt institutionnel numérique de lULB, est loutil de référencementde la production scientifique de lULB.Linterface de recherche DI-fusion permet de consulter les publications des chercheurs de lULB et les thèses qui y ont été défendues.
We investigated, by in situ hybridization histochemistry, the cellular localization of the mRNA encoding a vitamin D-dependent calcium-binding protein (calbindin D28) in rat brain and peripheral organs. Using a [35S]cRNA probe under high stringency conditions, specific mRNA was found in tissues well …
Recombinant human Calbindin D9K was cloned from bovine cDNA and expressed inE.coli. It bounds to one Ca and one Ln ion. Two calcium ions are bound in the Calbindin D9K version. The protein consists of Calbindin D9K, mutant P47M (residues 5-79, swissprot a
Although Calbindin-D9k (CaBP-9k), a cytosolic calcium binding protein which has calcium binding sites, is expressed in various tissues, i.e., intestine, uterus, and placenta, potential roles of this...
The vomeronasal organs (VNOs) of two humans, a male neonate and a female adult, were examined for immunolocalization of calbindin-D28k (calbindin) which has been immunolocalized to VNO receptor cells in other mammals. The present study demonstrates that epithelial cells within the VNOs of both subjects expressed calbindin-like immunoreactivity. These results suggest that human VNO epithelial cells of both genders express calbindin during development and in the adult.
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Reagents for the antigen Basalin / S100 calcium binding protein A17 / S100A17 / trichohyalin-like 1 / TCHHL1 / THHL1 stained with Cy5™ in the Antibody Database
Buy our Recombinant Human S100 calcium binding protein A14. Ab101041 is a full length protein produced in Escherichia coli and has been validated in WB…
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Calcium binding protein information including symptoms, causes, diseases, symptoms, treatments, and other medical and health issues.
The structure of calbindin is characteristic of an EF-hand protein, with two helix-loop-helix calcium binding motifs joined by a flexible linker, and a short anti-parallel beta-type interaction between the two ion-binding sites. The structure of calbindin has been solved in the apo (Conformation 1), (Ca2+)(2) (Conformation 2), and (Cd2+)(1) (Conformation 3) states in x-ray and NMR studies. Upon binding of both Calcium ions to the structure, the two ion-binding loops in the structure move approximately 1.00 A together, while the pairs of helices in the structure move approximately 0.45 A together ...
Discussion. Study design. In this study, we describe the analysis of 22K gene expression profiles of macular and peripheral RPE cells. There have been a number of other published studies that had a similar research question but employed different methodologies [8,17-21]. However, all of these studies suffered from one or more relative weaknesses in terms of sample selection, RPE cell handling, target selection, as well as microarray- or bioinformatics methods. Our aim was to avoid these limitations as much as possible (Table 5 and Table 6).. While the number of samples we used was limited, our entire strategy was focused on reduction of gene expression differences between individual eyes. In this study, we detected only consistent differences, more or less present in the RPE of each individual eye. We neither detected transient gene expression differences nor potential differences which co-occurred with high interindividual variation. The complexity of our material was greatly reduced by ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
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Contact us today for a free consultation with the scientific team and discover how Creative Biogene can be a valuable resource and partner for your organization.. ...
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Parvalbumin alpha / PVALB, 0.1 ml. Parvalbumin?is a?calcium-binding?albumin?protein with low molecular weight (typically 9-11 kDa).
pep:known chromosome:VEGA66:3:90511034:90514392:-1 gene:OTTMUSG00000022107 transcript:OTTMUST00000052482 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:S100a1 description:S100 calcium binding protein A1 ...
S100A12 - S100A12 (untagged)-Human S100 calcium binding protein A12 (S100A12) available for purchase from OriGene - Your Gene Company.
Recombinant protein of human S100 calcium binding protein A11 (S100A11), 20 ug available for purchase from OriGene - Your Gene Company.
We have isolated a full-length cDNA clone for a novel 29 kDa protein that is highly expressed in rat enamel cells. The clone… Expand ...
1α,25-Dihydroxyvitamin D3 [1,25(OH)2D3] is known to modulate Ca2+ metabolism in several cell types. Vitamin-D-dependent calcium binding proteins such as calbindin-D28K (28 kDa calcium binding proteins) have been shown to be regulated by 1,25(OH)2D3 but the mechanisms controlling calbindin synthesis are still poorly understood in human osteoblast cell culture models. The human bone marrow stromal cells (HBMSC) described in this paper developed a calcified matrix, expressed osteocalcin (OC), osteopontin (OP) and responded to 1,25(OH)2D3. The expression of vitamin D receptor mRNA was demonstrated by reverse transcription-PCR. Calbindin-D28K protein was identified only in cells arising from the sixth subculture, which exhibited a calcified matrix and all of the osteoblastic markers, e.g. OC and OP. It was demonstrated by dot-immunodetection using immunological probes, and by in situ hybridization using labelled cDNA probes. Moreover, vitamin D3 enhanced calbindin-D28K synthesis as well as OC ...
Looking for online definition of S100 calcium binding protein A3 in the Medical Dictionary? S100 calcium binding protein A3 explanation free. What is S100 calcium binding protein A3? Meaning of S100 calcium binding protein A3 medical term. What does S100 calcium binding protein A3 mean?
Animals. Two strains of transgenic mice, each bearing a jellyfish green fluorescent protein (GFP) reporter, were used. The mPer1::d2EGFP transgenic mouse (termed here Per1::GFP; a gift from Dr. D. McMahon, Vanderbilt University, Nashville, TN) was made with the B6C3F1 hybrid mouse as described previously (Kuhlman et al., 2000). The mice used in the present studies were hemizygous for the d2EGFP transgene, which is a degradable form of GFP.. The calbindin-D28K-bacterial artificial chromosome (BAC)::GFP transgenic mouse (termed here CalB::GFP; a gift from Dr. N. Heintz, Rockefeller University, New York, NY) was made by insertion of enhanced GFP (EGFP) (Yang et al., 1997) in the calbindin BAC (Research Genetics/Invitrogen, Carlsbad, CA) by homologous recombination. The homologous region was upstream of the start codon of the calbindin gene. The first stretch of the sequence included the calbindin-D28K promoter followed by the first linker sequence and then the EGFP construct followed by the second ...
Motor neurons become hyperexcitable during progression of amyotrophic lateral sclerosis (ALS). This abnormal firing behavior has been explained by changes in their membrane properties, but more recently it has been suggested that changes in premotor circuits may also contribute to this abnormal activity. The specific circuits that may be altered during development of ALS have not been investigated. Here we examined the Renshaw cell recurrent circuit that exerts inhibitory feedback control on motor neuron firing. Using two markers for Renshaw cells (calbindin and cholinergic nicotinic receptor subunit alpha2 [Chrna2]), two general markers for motor neurons (NeuN and vesicular acethylcholine transporter [VAChT]), and two markers for fast motor neurons (Chondrolectin and calcitonin-related polypeptide alpha [Calca]), we analyzed the survival and connectivity of these cells during disease progression in the Sod1G93A mouse model. Most calbindin-immunoreactive (IR) Renshaw cells survive to end stage but
The encoded protein is a secreted calcium-binding protein which is found in the cytoplasm. It is related to calbindin D-28K and calretinin. This protein is thought to be involved in potassium chloride-stimulated calcium flux and cell proliferation.[6] This protein plays an important role in the release of the stress hormone Corticotropin-releasing hormone (CRH) and which only then enables stress processes in the brain. ...
At least three CaBPs are abundant in various types of nerve cells : calbindin-D28, calretinin, and parvalbumin. The sequence of chick calretinin, from cDNA clones, is 60% homologous to that of chick...
The present investigation has demonstrated that ionizing radiation in the therapeutic dose range stimulates a transient cellular generation of ROS/RNS. Temporally coincident is a radiation-induced reversible depolarization of the mitochondrial ΔΨ and decrease in mitochondrial entrapped calcein fluorescence, both hallmarks of the mitochondrial permeability transition. The amount of ROS/RNS generated is relatively constant over the dose range tested, but the number of cells that respond increases with the dose. The radiation-induced ROS/RNS generation, ΔΨ depolarization, and calcein release are inhibited by CsA but not by the structural analogue, CsH. Overexpression of the Ca2+-binding protein calbindin 28K or treatment of cells with BAPTA/AM, an intracellular Ca2+ chelator, also effectively block radiation-induced ROS/RNS. The increased ROS/RNS generation observed with radiation is common to all of the cell types examined but for one important exception, the mitochondrial DNA-less ρo ...
Here, we provided new as well as conclusive supporting evidence indicating that Pax6-dependent mechanisms control the generation of distinct neural sublineages of the MZ, IGLs, and SGLs in the developing cortex and possibly after birth.. In the absence of Pax6, the number of Reelin+/Calretinin+ cells in the MZ of Sey/Sey mutant progressively increases during embryogenesis based on a non-cell-autonomous mechanism that appears to include migration of Reelin- and Calretinin-positive cells accumulated in the OB remnants located in the lateral telencephalon (Jiménez et al., 2000; Stoykova et al., 2003). Because the Emx1-Cre line does not drive inactivation of the early expression of Pax6 in the olfactory placode, Emx1-Cre/Pax6cKO mice have differentiated OBs at a normal location. Despite this, the number of Reelin+, Calretinin+, and Tbr1+ cells in MZ was significantly augmented in these mutants. Several lines of evidence indicated the existence of a common progenitor pool in cortical VZ, capable of ...
The idea of Professors Bolis and Gilles to gather together for a 3 days meeting in the splendid environment of Crans-Montana in Switzerland a limited number of people around the subject of calcium an
Cornelissen, WB; de Laet, AB; Kroese, AB; Adriaensen, DW; van Bogaert, PP; Scheuermann, DW; Timmermans, JP (Oct 1999). Species-dependent features of Dogiel type II neurones in the mammalian enteric nervous system. European Journal of Morphology. 37 (4-5): 241-9. doi:10.1076/ejom.37.4.241.4730. PMID 10477469 ...
casSAR Dugability of Q9DB16 | Cab39l | Calcium-binding protein 39-like - Also known as CB39L_MOUSE, Cab39l. Component of a complex that binds and activates STK11/LKB1. In the complex, required to stabilize the interaction between CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta) and STK11/LKB1 (By similarity). Component of a trimeric complex composed of STK11/LKB1, STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta): the complex tethers STK11/LKB1 in the cytoplasm and stimulates its catalytic activity.
CAB39 antibody (calcium binding protein 39) for ICC/IF, IHC-P, WB. Anti-CAB39 pAb (GTX110628) is tested in Human, Mouse samples. 100% Ab-Assurance.
Sigma-Aldrich offers abstracts and full-text articles by [Leyuan Bao, Adam F Odell, Sam L Stephen, Stephen B Wheatcroft, John H Walker, Sreenivasan Ponnambalam].
To date, unequivocal neuroanatomical features have been demonstrated neither for sporadic nor for familial schizophrenia. Here, we investigated the neuroanatomical changes in a transgenic rat model for a subset of sporadic chronic mental illness (CMI), which modestly overexpresses human full-length, non-mutant Disrupted-in-Schizophrenia 1 (DISC1), and for which aberrant dopamine homeostasis consistent with some schizophrenia phenotypes has previously been reported. Neuroanatomical analysis revealed a reduced density of dopaminergic neurons in the substantia nigra and reduced dopaminergic fibres in the striatum. Parvalbumin-positive interneuron occurrence in the somatosensory cortex was shifted from layers II/III to V/VI, and the number of calbindin-positive interneurons was slightly decreased. Reduced corpus callosum thickness confirmed trend-level observations from in vivo MRI and voxel-wise tensor based morphometry. These neuroanatomical changes help explain functional phenotypes of this ...
Osteopathic Medicine;Vitamin D-Binding Protein;Vitamin D Deficiency;Vitamin D;Hypercalcemia;S100 Calcium Binding Protein G;Bone Diseases;Calbindins;Calcifediol;Calcification, Physiologic;Gene Targeting;Hyperparathyroidism;Parathyroid Hormone;RNA ...
Osteopathic Medicine;Vitamin D-Binding Protein;Vitamin D Deficiency;Vitamin D;Hypercalcemia;S100 Calcium Binding Protein G;Bone Diseases;Calbindins;Calcifediol;Calcification, Physiologic;Gene Targeting;Hyperparathyroidism;Parathyroid Hormone;RNA ...
Striatal fast spiking interneurons (FSI) modulate the output of the striatum by synchronizing medium-sized spiny neurons (MSN). Recent studies have broadened our understanding of FSI, showing that they are implicated in severe motor disorders such as Parkinsonism, dystonia and Tourette syndrome. FSI are the only striatal neurons to express the calcium-binding protein parvalbumin (PV). This selective expression of PV raises questions about the functional role of this Ca2+ buffer in controlling FSI Ca2+ dynamics, and, consequently, the FSI spiking mode and neurotransmission.
Worldwide expansion of mobile phones and electromagnetic field (EMF) exposure has raised question of their possible biological effects on the brain and nervous system. Radiofrequency (RF) radiation might alter intracellular signaling pathways through changes in calcium (Ca(2+)) permeability across cell membranes. Changes in the expression of calcium binding proteins (CaBP) like calbindin D28-k (CB) and calretinin (CR) could indicate impaired Ca(2+)homeostasis due to EMF exposure. CB and CR expression were measured with immunohistochemistry in the hippocampus of mice after EMF exposure at 835 MHz for different exposure times and absorption rates, 1 h/day for 5 days at a specific absorption rate (SAR)=1.6 W/kg, 1 h/day for 5 days at SAR=4.0 W/kg, 5 h/day for 1 day at SAR=1.6 W/kg, 5 h/day for 1 day at SAR=4.0 W/kg, daily exposure for 1 month at SAR=1.6 W/kg. Body weights did not change significantly. CB immunoreactivity (IR) displayed moderate staining of cells in the cornu ammonis (CA) areas and ...
S100B (S100 calcium binding protein B), Authors: M Rosario Fernandez-Fernandez, Alan R Fersht. Published in: Atlas Genet Cytogenet Oncol Haematol.
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Shortall, S. E., Brown, A. M., Newton-Mann, E., Dawe-Lane, E., Evans, C., Fowler, M., & King, M. V. (2020). Calbindin Deficits May Underlie Dissociable Effects of 5-HT6 and mGlu7 Antagonists on Glutamate and Cognition in a Dual-Hit Neurodevelopmental Model for Schizophrenia. Molecular Neurobiology, https://doi.org/10.1007/s12035-020-01938-x © 2020, The Author(s). Despite several compounds entering clinical trials for the negative and cognitive symptoms of schizophrenia, few have progressed beyond phase III. This is partly attributed to a need for improved preclinical models, to understa... Read More about Calbindin Deficits May Underlie Dissociable Effects of 5-HT6 and mGlu7 Antagonists on Glutamate and Cognition in a Dual-Hit Neurodevelopmental Model for Schizophrenia. ...
Calcium-Binding Proteins: Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS.
Buy the Aqua Vision Aquatics Precision Integreat (Part A & B Calcium/Buffer) - 32 oz. Each for your aquarium and read product reviews, watch videos and see detailed specs at MarineDepot.com.
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
InitialConditions, ,ConcentrationSet region=dend, ,!-- calcium interacting proteins --, ,NanoMolarity specieID=Ca value=60/, ,!-- total calbindin = 160 uM --, ,NanoMolarity specieID=Calbin value=159980/, ,NanoMolarity specieID=CalbinC value=20/, ,!-- total calmodulin = 9000 nM (divide AC bound by 3.25)--, ,NanoMolarity specieID=Cam value=3590/, ,NanoMolarity specieID=CamCa2C value=126/, ,NanoMolarity specieID=CamCa2N value=20/, ,NanoMolarity specieID=CamCa4 value=0/, ,!-- total PP2B = 5000 nM--, ,NanoMolarity specieID=PP2B value=96 /, ,NanoMolarity specieID=PP2BCam value=3482 /, ,NanoMolarity specieID=PP2BCamCa2C value=1231 /, ,NanoMolarity specieID=PP2BCamCa2N value=181 /, ,NanoMolarity specieID=PP2BCamCa4 value=10 /, ,NanoMolarity specieID=L value=2.0/, ,NanoMolarity specieID=ATP value=1998000.0/, ,NanoMolarity specieID=cAMP value=89.0/, ,NanoMolarity specieID=AMP value=0.0/, ,NanoMolarity specieID=Epac1cAMP value=35.0/, ...
Complete information for S100G gene (Protein Coding), S100 Calcium Binding Protein G, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Ca2+ and Mg2+ are essential ions in a wide variety of cellular processes and form a major constituent of bone. It is, therefore, essential that the balance of these ions is strictly maintained. In the last decade, major breakthrough discoveries have vastly expanded our knowledge of the mechanisms un …
Use Bio-Rads PrimePCR assays, controls, templates for your target gene. Every primer pair is optimized, experimentally validated, and performance guaranteed.
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Calbindin Calmodulin Calsequestrin Kinjo, Tashi G; Schnetkamp, Paul PM. Ca2+ Chemistry, Storage and Transport in Biologic ... Compartmentalization of calcium binding proteins such as calretinin and calbindin-28 kDa has been noted within cells, ... calretinin and calbindin-28 kDa, in ganglion and amacrine cells of the rat retina". Molecular Vision. 14: 1600-1613. PMC ...
The territory is stained for calbindin. The mediator of the pallido-thalamic connection is the inhibitor GABA. The ...
The VL is stained for calbindin and acetylcholinesterase. The axons ascend in the nucleus where they branch profusely. The VL ... François, C.; Yelnik, J.; Percheron, G.; Tandé, D. (1994). "Calbindin-D-28K as a marker of the associative coertical territory ...
In the rat, few also express calbindin (CB) or calretinin (CR). Many of the DTN GABAergic cells do express parvalbumin (PV) ...
Vitamin D also increases transcription of genes that code for Calbindin. As the name implies, Calbindin functions as a calcium- ...
Lee, Y. S.; Taylor, A. N.; Reimers, T. J.; Edelstein, S.; Fullmer, C. S.; Wasserman, R. H. (1987). "Calbindin-D in peripheral ... Fullmer, C. S.; Wasserman, R. H. (1987). "Chicken intestinal 28-kilodalton calbindin-D: Complete amino acid sequence and ... known today as calbindin. In the mid-1970s, Wasserman developed an interest in the hypercalcemic effects of extracts of the ... calbindin-D28k, and parvalbumin in Purkinje cells of avian and mammalian cerebellum". Proceedings of the National Academy of ...
Those in the upper layer (or dorsal tier) contain a binding protein called calbindin-D28K which can buffer calcium levels ... Nemoto, C.; Hida, T.; Arai, R. (1999-10-30). "Calretinin and calbindin-D28k in dopaminergic neurons of the rat midbrain: a ... "Calbindin content and differential vulnerability of midbrain efferent dopaminergic neurons in macaques". Frontiers in ... dense μ-opioid receptor staining embedded within a matrix compartment that contains higher acetylcholinesterase and calbindin- ...
A specific, recognizable feature of Purkinje neurons is the expression of calbindin. Calbindin staining of rat brain after ... Whitney ER, Kemper TL, Rosene DL, Bauman ML, Blatt GJ (February 2008). "Calbindin-D28k is a more reliable marker of human ...
Calbindin is expressed by cerebellar Purkinje cells and granule cells of the hippocampus. The reorganization and migration of ... calbindin-stained Purkinje neurons in rat cerebellum after peripheral nerve injury suggests that calbindin may be a marker for ...
... s express somatostatin and sometimes calbindin, but not parvalbumin or vasoactive intestinal peptide. ...
Calbindin is a protein involved in calcium ion transport within cells, and excess calcium in cells is toxic. The calbindin ... Secondly, dopaminergic neurons in the pars compacta contain less calbindin than other dopaminergic neurons. ... "Midbrain dopaminergic neurons in the mouse that contain calbindin-D28k exhibit reduced vulnerability to MPTP-induced ...
However, some reports have indicated that Renshaw cells synthesize calcium-binding proteins calbindin-D28k and parvalbumin.[ ...
"Choline deficiency alters global histone methylation and epigenetic marking at the Re1 site of the calbindin 1 gene". FASEB J. ...
Woo TU, Shrestha K, Lamb D, Minns MM, Benes FM (April 2008). "N-Methyl-D-Aspartate Receptor and Calbindin-Containing Neurons in ... calbindin, targeting the dendrites of pyramidal neurons, and the expression of the mRNA for the GluR5 kainate receptor in GABA ...
Alternating compartments of dense Cat-301 and calbindin immunore- activity in layer 4A". Cerebral Cortex. 12 (7): 671-691. doi: ...
VTA neurons are protective against MPP+ insult due to the expression of calbindin. Calbindin regulates the availability of Ca2+ ...
Vo T. T. B., Jeung E. B. (2009) An Evaluation of Estrogenic Activity of Parabens Using Uterine Calbindin-D9k Gene in an ...
... by their expression of calbindin and calretinin. In the reeler DG, the two cell types intermingle. Impaired dendrite outgrowth ...
... including calbindins and S100G Vitamin D resistant rickets, X-linked hypophosphatemia, a form of rickets for which ingestion of ...
... and calbindin (25%) and resembles alpha-parvalbumin in its N-terminal region (52%). It has a 40-residue N-terminal domain with ...
There are three proteins by which K cells can be clearly distinguished: Calbindin (28kDa calcium binding protein, CALB) The ...
It is encoded in humans by the CALB2 gene and was formerly known as calbindin-D29k. S100G, formerly calbindin 3 and calbindin- ... Calbindin 1 or simply calbindin was first shown to be present in the intestine in birds and then found in the mammalian kidney ... Calbindins are three different calcium-binding proteins: calbindin, calretinin and S100G. They were originally described as ... and calbindin 1 has six. Unlike calbindin 1 and 2, S100G is a member of the S100 family of calcium-binding proteins. S100G ...
... or calbindin-immuno-reactive neurons (CIR neurons), are widely distributed and diffusely dispersed in each of the nuclei of the ...
... by the intestine via calbindin. ...
Calbindin. *Calcineurin. *Calcitonin gene-related peptide. *Calcium-activated potassium channel subunit alpha-1 ...
... is a calcium-binding protein of the sarcoplasmic reticulum. The protein helps hold calcium in the cisterna of the sarcoplasmic reticulum after a muscle contraction, even though the concentration of calcium in the sarcoplasmic reticulum is much higher than in the cytosol. It also helps the sarcoplasmic reticulum store an extraordinarily high amount of calcium ions. Each molecule of calsequestrin can bind 18 to 50 Ca2+ ions.[1] Sequence analysis has suggested that calcium is not bound in distinct pockets via EF-hand motifs, but rather via presentation of a charged protein surface. Two forms of calsequestrin have been identified. The cardiac form Calsequestrin-2 (CASQ2) is present in cardiac and slow skeletal muscle and the fast skeletal form Calsequestrin-1(CASQ1) is found in fast skeletal muscle. The release of calsequestrin-bound calcium (through a calcium release channel) triggers muscle contraction. The active protein is not highly structured, more than 50% of it adopting a ...
It is related to calbindin D-28K and calretinin. This protein is thought to be involved in potassium chloride-stimulated ...
... calbindin expressed in distal tubules and aquaporin 2 expressed in the collecting duct cells.[14] ...
骨化三醇通过激活维生素D受体发挥生物学效应,VDR存在于靶细胞表面,[4
... such as TRPV6 and calbindin), which are involved in calcium absorption in the intestine.[17] The vitamin D receptor belongs to ...
It is encoded in humans by the CALB2 gene and was formerly known as calbindin-D29k. S100G, formerly calbindin 3 and calbindin- ... Calbindin 1 or simply calbindin was first shown to be present in the intestine in birds and then found in the mammalian kidney ... Calbindins are three different calcium-binding proteins: calbindin, calretinin and S100G. They were originally described as ... and calbindin 1 has six. Unlike calbindin 1 and 2, S100G is a member of the S100 family of calcium-binding proteins. S100G ...
... Steven Roy Daviss sdaviss at COSY.AB.UMD.EDU Wed Nov 17 09:49:08 EST 1993 *Previous message: ... See Lund and Lewis, JCN, 1993, for description of these in primates.) We found that calbindin-ir neurons were 50-70% increased ... those immunoreactive for CALBINDIN and for CALRETININ. ( ...
calbindin. Names. RTVL-H protein. calbindin 1, (28kD). calbindin 1, 28kDa. calbindin 27. calbindin D28. vitamin D-dependent ... CSF calbindin concentration is a sensitive biomarker of NPC1 disease Title: Cerebrospinal Fluid Calbindin D Concentration as a ... CALB1 calbindin 1 [Homo sapiens] CALB1 calbindin 1 [Homo sapiens]. Gene ID:793 ... calbindin 1provided by HGNC. Primary source. HGNC:HGNC:1434 See related. Ensembl:ENSG00000104327 MIM:114050 Gene type. protein ...
Notably, increasing DG calbindin levels, either by direct virus-mediated expression or inhibition of ΔFosB signaling, improves ... Hippocampal inhibition of ΔFosB or elevation of calbindin rescues spatial memory deficits in mouse models of AD. The calcium- ... Moreover, levels of ΔFosB and calbindin expression are inversely related in the DG of individuals with temporal lobe epilepsy ( ... However, despite the importance of calbindin in both neuronal physiology and pathology, the regulatory mechanisms that control ...
Calbindin D-28k occurs in all major pathways of the limbic system with the exception of the fornix. Calbindin D-28k is, however ... Springer, Berlin) is very rich in calbindin D-28k. The distribution of calbindin D-28k-positive neurons is very similar to that ... Calbindin D-28k is primarily associated with long-axon neurons (Golgi type I cells) exemplified by thalamic projection neurons ... Calbindin D-28k and parvalbumin in the rat nervous system.. Celio MR1. ...
K. Sooy, J. Kohut, and S. Christakos, "The role of calbindin and 1,25dihydroxyvitamin D. 3. in the kidney," Current Opinion in ... M. Takashi, Y. Zhu, K. Miyake, and K. Kato, "Urinary 28-kD calbindin-D as a new marker for damage to distal renal tubules ... Meso Scale Discovery and Luminex Comparative Analysis of Calbindin D28K. Samer Sourial, Maritha Marcusson-Ståhl, and Karin ... S. Hasegawa, K. Kato, M. Takashi et al., "Increased levels of calbindin-D in serum and urine from patients treated by ...
1IG5: Structural basis for the negative allostery between Ca(2+)- and Mg(2+)-binding in the intracellular Ca(2+)-receptor calbindin D9k.
... calbindin-D9k explanation free. What is calbindin-D9k? Meaning of calbindin-D9k medical term. What does calbindin-D9k mean? ... Looking for online definition of calbindin-D9k in the Medical Dictionary? ... calbindin-D9k. calbindin-D9k. A calcium-binding protein encoded by S100G, which is found in the intestinal epithelial cells. It ... Calbindin-D9k , definition of calbindin-D9k by Medical dictionary https://medical-dictionary.thefreedictionary.com/calbindin- ...
There is a paucity of information on the effects of calcium binding on calbindin D-28K structure. To further examine the ... Calbindin D-28K is a six-EF-hand calcium-binding protein found in the brain, peripheral nervous system, kidney, and intestine. ... mechanism and structural consequences of calcium binding to calbindin D-28K we performed detailed complementary heteronuclear ... Calbindin D-28K is a six-EF-hand calcium-binding protein found in the brain, peripheral nervous system, kidney, and intestine. ...
... calbindin D28) in rat brain and peripheral organs. Using a [35S]cRNA probe under high stringency conditions, specific mRNA was ... Localization of calbindin D28 mRNA in rat tissues by in situ hybridization Neurosci Lett. 1988 Mar 31;86(2):155-60. doi: ... Using a [35S]cRNA probe under high stringency conditions, specific mRNA was found in tissues well known for their calbindin D28 ... In situ hybridization histochemistry allows the precise identification of cells expressing calbindin D28 and offers a new ...
Calretinin, CR, Calb2, calbindin 2.. Introduction. Calretinin is an intracellular calcium-binding protein belonging to the ...
Rabbit Polyclonal Anti-Calbindin D-28K Antibody - Pre-diluted. Validated: IHC, IHC-P. Tested Reactivity: Human, Mouse, Rat. 100 ... Additional Calbindin D-28K Products. Calbindin D-28K NBP1-35393 * Calbindin D-28K Antibodies ... Home » Calbindin D-28K » Calbindin D-28K Antibodies » Calbindin D-28K Antibody - Pre-diluted ... Blogs on Calbindin D-28K. There are no specific blogs for Calbindin D-28K, but you can read our latest blog posts. ...
Find and compare multiple sources of anti-Calbindin antibody using the Linscotts Directory search engine. Monoclonal, ... Calbindin (Calbindin D28, D-28K, Spot 35 Protein, Vitamin D-dependent Calcium-binding Protein, Avian-type, Calb1). ... Recognizes mouse Calbindin (CALB). Immunogen. CALB (Glu3~Asn261); MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA ...
Research proven mouse monoclonal Calbindin D anibody. EXcellent for immunohistochemistry, western blotting, ELISA and related ... Calbindin-D, also known as Calbindin 1, is a Vitamin D-dependent cytoplasmic protein in the EF-hand calcium binding protein ... Calbindin-D is widely expressed and regulates calcium homeostasis. Calbindin-D protects neurons from excitotoxic and apoptotic ... Calbindin D staining of paraffin-embedded human cerebellum and frozen rat hypothalamus sections using 25 µg/mL mouse anti-human ...
The solution structures of mutant calbindin D9ks, as determined by NMR, show that the calcium-binding site can adopt different ... CALBINDIN D9K A 76 Bos taurus Mutation: A15D, P20G, P43M Gene Name(s): S100G Gene View CALB3 S100D ... THE SOLUTION STRUCTURES OF MUTANT CALBINDIN D9KS, AS DETERMINED BY NMR, SHOW THAT THE CALCIUM BINDING SITE CAN ADOPT DIFFERENT ...
Calbindin. Calbindin je termín označující skupinu transportních proteinů v buňkách tenkého střeva a v ledvinách. Tyto proteiny ...
We report studies of the human calbindin-D9k promoter. Differences between the reported sequences of the human calbindin-D9k ... Factors involved in the duodenal expression of the human calbindin-D9k gene. Natalie F. BARLEY, S. Radhika PRATHALINGAM, Pang ... Calbindin-D9k is expressed in the cytoplasm of intestinal cells, where it is critical for dietary calcium absorption. Two ... The calbindin-D9k gene contains multiple potential binding sites for homeobox transcription factors; one of these, known as IPF ...
To further investigate the role of calbindin D28k in Alzheimers disease (AD); hippocampus, superior temporal gyrus and ... Calbindin D28k mRNA in hippocampus, superior temporal gyrus and cerebellum: comparison between control and Alzheimer disease ... To further investigate the role of calbindin D28k in Alzheimers disease (AD); hippocampus, superior temporal gyrus and ...
Calbindin-D28k is unique in that it functions as both a calcium buffer and a sensor protein. It is found in many tissues, ... Calbindin-D28k is known to bind four calcium ions and upon calcium binding undergoes a conformational ... Calbindin-D28k is a calcium binding protein with six EF hand domains. ... Calbindin-D28k is a calcium binding protein with six EF hand domains. Calbindin-D28k is unique in that it functions as both a ...
Synthesis of calbindin-D28K during mineralization in human bone marrow stromal cells. Corinne FAUCHEUX, Reine BAREILLE, Joëlle ... Calbindin-D28K protein was identified only in cells arising from the sixth subculture, which exhibited a calcified matrix and ... In conclusion, the studies in vitro described in the present paper indicate, for the first time, a possible role of calbindin- ... Moreover, vitamin D3 enhanced calbindin-D28K synthesis as well as OC synthesis and alkaline phosphatase activity. Uptake of 45 ...
Overall levels of calbindin were higher in the HP of corticosterone-treated birds, due almost entirely to elevated calbindin ... Calbindin-D28k is constitutively expressed in cells of the nervous system but increases in concentration following a neurotoxic ... Calbindin-D28k Expression Increases in the Dorsolateral Hippocampus Following Corticosterone Treatment in Female Zebra Finches ... We hypothesized that treatment of female zebra finches with a glucocorticoid (corticosterone) would increase calbindin ...
Calbindin D-28k and parvalbumin immunoreactivity in the frontal cortex in patients with frontal lobe dementia of non-Alzheimer ... Calbindin D-28k and parvalbumin immunoreactivity in the frontal cortex in patients with frontal lobe dementia of non-Alzheimer ... Calbindin D-28k and parvalbumin immunoreactivity in the frontal cortex in patients with frontal lobe dementia of non-Alzheimer ...
Calbindin 28K and calretinin are very similar calcium binding proteins which are both present in the central nervous system ( ... Calbindin 28K is also present in the endocrine system. We have examined the cellular distribution of calbindin in the ... Calbindin 28K and calretinin are very similar calcium binding proteins which are both present in the central nervous system ( ... Pochet R, Pipeleers DG and Malaisse WJ (1987). Calbindin D 27 kDa: preferential localization in non-B islet cells of the rat ...
Calbindin D-28k Parvalbumin GABA MeSH Terms expand_less. expand_more. Animals Calbindins Calcium Carrier Proteins Cats ... 5. Calbindin D-28k and parvalbumin-immunoreactive cells were round, oval, spindle or polygonal in shape and were 15~20 ... 3. Calbindin immunoreactive cells in the substans nigr were more than twice in number compared to the parvalbumin ... Immunocytochemical Studies of Calbindin D-28k and Parvalbumin-Containing GABAergic Neurons in the Midbrain of the Cat. ...
Although Calbindin-D9k (CaBP-9k), a cytosolic calcium binding protein which has calcium binding sites, is expressed in various ... Reproduced with permission from Yun S-M, Choi KC, Kim IH, An BS, Lee GS, Hong EJ, Son JH, Oh GT, Jeung E-B 2004 Calbindin-D9k ... Yun SM, Choi KC, Kim IH, An BS, Lee GS, Hong EJ, Oh GT, Jeung EB: Dominant expression of porcine Calbindin-D9k in the uterus ... An BS, Choi KC, Kang SK, Lee GS, Hong EJ, Hwang WS, Jeung EB: Mouse calbindin-D(9k) gene expression in the uterus during late ...
Calbindin-D28k Antibody 14479-1-AP has been identified with IF, IHC, IP, WB, ELISA. 14479-1-AP detected 28 kDa band in mouse ... Calbindin-D28k Antibody 3 Publications. Rabbit Polyclonal, Catalog number: 14479-1-AP Featured Product KD/KO validated ... IHC result of Calbindin antibody (14479-1-AP, 1:5000 in 1% BSA 0.3%Tx-100 Tris buffer incubated at 4℃ overnight) with freshly ... IHC result of Calbindin antibody (14479-1-AP, 1:5000 in 1% BSA 0.3%Tx-100 Tris buffer incubated at 4℃ overnight) with freshly ...
A distinct group of calbindin-IR neurons was present in the ventral medulla. Seventy-five percent of these calbindin-IR neurons ... A distinct group of calbindin-IR neurons was present in the ventral medulla. Seventy-five percent of these calbindin-IR neurons ... Immunocytochemistry was used to reveal calbindin, TH, PNMT, and CTB immunoreactivity. Ten calbindin-IR cell groups were ... Immunocytochemistry was used to reveal calbindin, TH, PNMT, and CTB immunoreactivity. Ten calbindin-IR cell groups were ...
Sprekeler, N; Kowalewski, M P; Boos, A (2012). TRPV6 and Calbindin-D9k-expression and localization in the bovine uterus and ... TRPV6 and Calbindin-D9k-expression and localization in the bovine uterus and placenta during pregnancy ... BACKGROUND: Transient receptor potential channel type 6 (TRPV6) and Calbindin-D9k (CaBP-9k) are involved in the active calcium ... BACKGROUND: Transient receptor potential channel type 6 (TRPV6) and Calbindin-D9k (CaBP-9k) are involved in the active calcium ...
Rabbit Calbindin Polyclonal Antibody-P05937.2 (MBS176994) product datasheet at MyBioSource, Primary Antibodies. Application: ... calbindin; D-28K; calbindin D28; RTVL-H protein; calbindin 1, (28kD); vitamin D-dependent calcium-binding protein, avian-type ... Figure 1. IHC analysis of Calbindin using anti-Calbindin antibody (MBS176994).. Calbindin was detected in paraffin-embedded ... Figure 2. IHC analysis of Calbindin using anti-Calbindin antibody (MBS176994).. Calbindin was detected in paraffin-embedded ...
Immunohistochemistry revealed changes in the chemical coding pattern of calbindin-immunoreactive neurons in the inferior ... neurons projecting to the colon exhibit calbindin-like immunoreactivity. It is not known if there are any changes in the ... animals and then tissue comprising IMGs from both groups was processed for double-labeling immunofluorescence with calbindin- ... A subpopulation of the pig inferior mesenteric ganglia (IMG) neurons projecting to the colon exhibit calbindin-like ...
  • While we did not use stereological techniques, we applied the same methodology to count TWO different subpopulations of nonpyramidal neurons--those immunoreactive for CALBINDIN and for CALRETININ. (bio.net)
  • We found that calbindin-ir neurons were 50-70% increased in density, while the calretinin-ir neurons were only 10-20% increased, in the setting of an 8-15% decrease in cortical thickness. (bio.net)
  • Society for Neuroscience Abstracts 1993, 84.9) It remains to be seen whether this change in the density of this subpopulation of GABAergic neurons is related to neuroleptic exposure, cortical area, or intracellular concentrations of calbindin. (bio.net)
  • Calbindin D-28k is primarily associated with long-axon neurons (Golgi type I cells) exemplified by thalamic projection neurons, strionigral neurons, nucleus basalis Meynert neurons, cerebellar Purkinje cells, large spinal-, retinal-, cochlear- and vestibular ganglion cells. (nih.gov)
  • The distribution of calbindin D-28k-positive neurons is very similar to that of the dihydroperydine subtype of calcium channels. (nih.gov)
  • Parvalbumin-immunoreactive neurons have a different, and mostly complementary distribution compared with those which react with calbindin D-28k antisera, but in a few cases (Purkinje cells of the cerebellum, spinal ganglion neurons), both calcium-binding proteins co-exist in the same neuron. (nih.gov)
  • Calbindin D28k is a member of a large family of intracellular calcium-binding proteins, containing EF-hand calcium binding motifs, and related to calmodulin and troponin-C. The biological roles of Calbindin D28k include calcium regulation and calcium-dependent signalling in neurons and during development. (novusbio.com)
  • Calbindin-D protects neurons from excitotoxic and apoptotic cell death by buffering excess calcium. (neuromics.com)
  • Calbindin-D28k is constitutively expressed in cells of the nervous system but increases in concentration following a neurotoxic insult, protecting neurons against apoptotic cell death. (ovid.com)
  • Calcium binding protein calbindin D-28k and parvalbumin immunoreactive neurons were mainly found in the red nucleus, substantia nigra, oculomotor nucleus and locus ceruleus of the cat midbrain. (koreamed.org)
  • The localisation of calbindin immunoreactivity in the medulla oblongata and its colocalisation with adrenaline-synthesising neurons [phenylethanolamine-N-methyltransferase-immunoreactive (PNMT-IR)] was examined (Granata and Chang [1994] Brain Res. (garvan.org.au)
  • However, detailed information about the distribution of calbindin-IR neurons in the reticular formation of the medulla oblongata in particular is lacking. (garvan.org.au)
  • In this report, the authors address this issue with an emphasis on the quantitation of calbindin-IR neurons, catecholamine neurons [tyrosine hydroxylase (TH)-IR, or PNMT-IR], and spinally projecting neurons in the ventral brainstem. (garvan.org.au)
  • Seven previously undescribed but distinct clusters of calbindin-IR neurons were found. (garvan.org.au)
  • Within the ventral pons, a population of calbindin-IR neurons occurred dorsal but adjacent to the A5 cell group. (garvan.org.au)
  • These calbindin-IR neurons did not contain either TH or PNMT immunoreactivity, and few if any of these neurons projected to the spinal cord. (garvan.org.au)
  • A distinct group of calbindin-IR neurons was present in the ventral medulla. (garvan.org.au)
  • Seventy-five percent of these calbindin-IR neurons contained TH immunoreactivity, 45% contained PNMT immunoreactivity, and 21% were spinally projecting neurons. (garvan.org.au)
  • Spinally projecting, calbindin-IR neurons were a subpopulation of PNMT-IR cells. (garvan.org.au)
  • In the intermediolateral cell column, close appositions of calbindin-IR terminals on identified sympathetic preganglionic neurons as well as calbindin-IR synapses indicated that these neurons may affect directly the sympathetic outflow. (garvan.org.au)
  • A subpopulation of the pig inferior mesenteric ganglia (IMG) neurons projecting to the colon exhibit calbindin-like immunoreactivity. (semanticscholar.org)
  • Increased number of calbindin neurons and fiber labeling both in focal cortical dysplasia (FCD) and non-FCD specimens compared with postmortem controls may be associated with ongoing seizure activity. (nih.gov)
  • Loss of calbindin-D28K is associated with the full range of tangle pathology within basal forebrain cholinergic neurons in Alzheimer's disease. (nih.gov)
  • This was also tested on neurons in culture where a clear difference between Calbindin-positive and -negative was seen at a concentration of 1:1000. (pabmabs.com)
  • In normal brain, calbindin (28kD) has been identified in medium sized neurons of the neuropil of the matrix compartment of the striatum, the woolly fiber arrangements of the globus pallidus and the fiber structures of the pars reticula of the substantia nigra. (antibody-antibodies.com)
  • In HD, alterations to the dendritic arbors and spiny striatal neurons may be visualized by immunohistochemistry for calbindin. (antibody-antibodies.com)
  • Different populations of parvalbumin- and calbindin-D28k-immunoreactive neurons contain GABA and accumulate 3H-D-aspartate in the dorsal horn of the rat spinal cord. (ox.ac.uk)
  • The colocalization of parvalbumin (PV), calbindin-D28k (CaBP), GABA immunoreactivities, and the ability to accumulate 3H-D-aspartate selectively were investigated in neurons of laminae I-IV of the dorsal horn of the rat spinal cord. (ox.ac.uk)
  • The cell body size and coexpression of carbonic anhydrase (CA), calretinin (CR) and calcitonin gene-related peptide (CGRP) of primary neurons with calbindin-D28k (CB) was examined in the trigeminal ganglion (TG) of the rat. (elsevier.com)
  • We have therefore re-examined the development of the murine SDN to determine whether it is influenced by AMH and/or whether the number of calbindin-positive (calbindin +ve ) neurons in it changes after pre-pubescent development. (biomedcentral.com)
  • In mice, the SDN nucleus is defined by calbindin +ve neurons (CALB-SDN). (biomedcentral.com)
  • The wild-type pre-pubertal male mice had 47% more calbindin +ve neurons in the CALB-SDN than their female wild-type littermates. (biomedcentral.com)
  • In adults, the extent of sexual dimorphism almost doubled due to a net reduction in the number and size of calbindin +ve neurons in females and a net increase in neuron number in males. (biomedcentral.com)
  • Consequently, the number of calbindin +ve neurons in Amh -/- adult male mice was intermediate between Amh +/+ males and Amh +/+ females. (biomedcentral.com)
  • The second phase of dimorphism is not dependent on the first phase having occurred as it was present in the Amh -/- male mice that have female-like numbers of calbindin +ve neurons at 20 days. (biomedcentral.com)
  • We report here that the number of calbindin-positive (calbindin +ve ) neurons in the sexually dimorphic nucleus of the medial preoptic area (CALB-SDN) of mice is dimorphic before puberty but with the extent of dimorphism subsequently doubling during or after puberty. (biomedcentral.com)
  • We studied the distributions of interneurons containing the calcium-binding proteins parvalbumin and calbindin D-28k, as well as that of pyramidal neurons containing nonphosphorylated neurofilament (NNF), in the middle temporal visual area (MT) of marmoset monkeys. (monash.edu)
  • Similar to the primary visual area (V1), in MT, calbindin-immunopositive neurons can be objectively classified into dark and light subtypes based on optical density of stained cell bodies. (monash.edu)
  • Calbindin-positive dark neurons are particularly concentrated in layers 2 and 3, whereas light neurons have a more widespread distribution. (monash.edu)
  • Calbindin-D28K immunoreactivity in the human cerebellar cortex. (nih.gov)
  • Decreased hippocampal expression of calbindin D28K and cognitive impairment in MELAS. (nih.gov)
  • Title: Role of Calbindin-D28k in Diabetes-Associated Advanced Glycation End-Products-Induced Renal Proximal Tubule Cell Injury. (nih.gov)
  • Westerink, R.H., Beekwilder, J.P. & Wadman, W.J. Differential alterations of synaptic plasticity in dentate gyrus and CA1 hippocampal area of calbindin-D28K knockout mice. (nature.com)
  • Deficits in memory and hippocampal long-term potentiation in mice with reduced calbindin D28K expression. (nature.com)
  • Decreases in Calbindin D28k abundance, or loss of Calbindin D28k immunoreactivity, is found in models of neurological disorders such as epilepsy, and in neurodegenerative diseases. (novusbio.com)
  • Calbindin-D28k is a calcium binding protein with six EF hand domains. (rti.org)
  • Calbindin-D28k is unique in that it functions as both a calcium buffer and a sensor protein. (rti.org)
  • Calbindin-D28k is known to bind four calcium ions and upon calcium binding undergoes a conformational change. (rti.org)
  • The structure of apo calbindin-D28k is in an ordered state, transitioning into a disordered state as calcium is bound. (rti.org)
  • The solution structure of disulfide-reduced holo-calbindin-D28k has been determined by NMR, while the structure of apo calbindin-D28k has yet to be determined. (rti.org)
  • Differential surface modification of lysine and histidine residues analyzed by mass spectrometry has been used in this study to identify, for the first time, the specific regions of calbindin-D28k undergoing conformational changes between the holo and apo states. (rti.org)
  • Using differential surface modification in combination with mass spectrometry, EF hands 1 and 4 as well as the linkers before EF hand 1 and the linkers between EF hands 4 and 5 and EF hands 5 and 6 were identified as regions of conformational change between apo and holo calbindin-D28k. (rti.org)
  • Vitamin-D-dependent calcium binding proteins such as calbindin-D28K (28 kDa calcium binding proteins) have been shown to be regulated by 1,25(OH) 2 D 3 but the mechanisms controlling calbindin synthesis are still poorly understood in human osteoblast cell culture models. (biochemj.org)
  • Calbindin-D28K protein was identified only in cells arising from the sixth subculture, which exhibited a calcified matrix and all of the osteoblastic markers, e.g. (biochemj.org)
  • Moreover, vitamin D 3 enhanced calbindin-D28K synthesis as well as OC synthesis and alkaline phosphatase activity. (biochemj.org)
  • In conclusion, the studies in v itro described in the present paper indicate, for the first time, a possible role of calbindin-D28K in mineralized matrix formation in HBMSC. (biochemj.org)
  • Calbindin-D28k is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. (ptglab.com)
  • Calbindin-D28k (calbindin) is a calcium-binding protein that is distributed widely in the rat brain. (garvan.org.au)
  • Significantly reduced hippocampal calbindin D28K levels are found in all patients with mitochondrial encephalomyopathy, lactic acidosis and stroke-like syndrome (MELAS). (nih.gov)
  • The family of EF-hand type Ca2+-binding proteins includes Calbindin D28K, Calbindin D9K, S-100 and , Calgranulin A (also designated MRP8), Calgranulin B (also designated MRP14), Calgranulin C and the Parvalbumin family members, including Parvalbumin and Parvalbumin (also designated oncomodulin). (abeomics.com)
  • Calbindin D28K, also known as calbindin, CALB1, D-28K or vitamin D-dependent calcium-binding protein, is a 261-amino acid protein with 6 EF-hand domains, 4 of which are active calcium-binding domains. (abeomics.com)
  • In neuronal cells, Calbindin D28K modulates calcium channel activity, calcium transients and intrinsic neuronal firing activity. (abeomics.com)
  • Also, Calbindin D28K has been implicated to play a role in apoptosis and microtubule function. (abeomics.com)
  • Expression of calbindin D28k (CB) immunoreactivity by putative Renshaw cells is substantially down-regulated by sciatic motoneuron axotomy in the adult rat. (ncl.ac.uk)
  • Crystallographic structure of calcium loaded Calbindin-D28K. (proteopedia.org)
  • Calbindin-D28K is a widely expressed calcium-buffering cytoplasmic protein that is involved in many physiological processes. (proteopedia.org)
  • Many of the targets of calbindin-D28K are of therapeutic interest: for example, inositol monophosphatase, the putative target of lithium therapy in bipolar disorder. (proteopedia.org)
  • Presented here is the first crystal structure of human calbindin-D28K. (proteopedia.org)
  • There are significant deviations in the tertiary structure when compared with the NMR structure of rat calbindin-D28K (PDB entry 2g9b), despite 98% sequence identity. (proteopedia.org)
  • Small-angle X-ray scattering (SAXS) indicates that the crystal structure better predicts the properties of calbindin-D28K in solution compared with the NMR structure. (proteopedia.org)
  • Here, the first direct visualization of the calcium-binding properties of calbindin-D28K is presented. (proteopedia.org)
  • SAXS data collected in both the presence and absence of calcium indicate that there are no large structural differences in the globular structure of calbindin-D28K between the calcium-loaded and unloaded proteins. (proteopedia.org)
  • Noble JW, Almalki R, Roe SM, Wagner A, Duman R, Atack JR. The X-ray structure of human calbindin-D28K: an improved model. (proteopedia.org)
  • The vomeronasal organs (VNOs) of two humans, a male neonate and a female adult, were examined for immunolocalization of calbindin-D28k (calbindin) which has been immunolocalized to VNO receptor cells in other mammals. (semanticscholar.org)
  • Both calbindin-D28k and parvalbumin-β were confined to hair cells in which they showed a similar distribution, whereas calretinin was present mainly in hair-cell nuclei but also occurred in supporting cells and nerve fibers. (jneurosci.org)
  • The hair-cell concentration of calbindin-D28k but not of parvalbumin-β increased from the low- to high-frequency end of the cochlea. (jneurosci.org)
  • Calibration against standards containing known amounts of calcium-buffering protein processed in the same fluid drop as the cochlear sections gave cytoplasmic concentrations of calbindin-D28k as 0.13-0.63 m m and parvalbumin-β as ∼0.25 m m , but calretinin was an order of magnitude less. (jneurosci.org)
  • We suggest that calbindin-D28k and parvalbumin-β may serve as endogenous mobile calcium buffers, but the predominantly nuclear location of calretinin argues for another role in calcium signaling. (jneurosci.org)
  • These values were derived from equivalent BAPTA concentrations (0.1-0.4 m m ) using simulations of stereociliary calcium gradients, assuming that calbindin-D28k has four Ca 2+ binding sites per molecule compared with one in BAPTA but binds Ca 2+ 25 times more slowly. (jneurosci.org)
  • the Calreticulin deficiency-mediated increase in cell death was not prevented by calbindin-D28k or Parvalbumin. (antibodies-online.com)
  • The effect(s) of oral calcium and vitamin D3 were examined on the expression of duodenal and renal active calcium transport genes, i.e., calbindin-D9k (CaBP-9k) and calbindin-D28k (CaBP-28k), transient receptor potential cation channels (TRPV5 and TRPV6), Na+/Ca2+ exchanger 1 (NCX1) and plasma membrane calcium ATPase 1b (PMCA1b), in CaBP-9k KO mice. (ewha.ac.kr)
  • Determination of Calcium-Binding Sites in Rat Brain Calbindin D28K by " by Timothy D. Veenstra, Kenneth L. Johnson et al. (cedarville.edu)
  • Calbindin D28K, a member of the troponin-C superfamily of calcium-binding proteins, contains six putative EF-hand domains. (cedarville.edu)
  • We used negative ion electrospray ionization mass spectrometry in order to definitively determine the exact calcium-binding stoichiometry of calbindin D28K and two mutant forms of the protein, one lacking EF-hand 2 (delta2) and the other lacking EF-hands 2 and 6 (delta2,6). (cedarville.edu)
  • Full-length calbindin D28K bound 4 mol of terbium/mol of protein, while calbindin delta2 and delta2,6 each bound 3 mol. (cedarville.edu)
  • These results clearly show that calbindin D28K binds 4 mol of calcium/mol of protein and that terbium-binding stoichiometry is similar to that of calcium. (cedarville.edu)
  • Corrigendum to "Inhibition of inositol monophosphatase (IMPase) at the calbindin-D28k binding site: Molecular and behavioural aspects" [Eur. (bgu.ac.il)
  • Additionally, calcium pump activity and calbindin-D28k expression were dramatically increased after GHE treatment, implicating that the modulation of calcium homeostasis could be involved in the mechanism underlying neuroprotection of GHE against ibotenic acid-induced excitotoxicity. (biomedsearch.com)
  • calmodulin, calbindin D28k and secretagogin. (dissertations.se)
  • Calbindins are three different calcium-binding proteins: calbindin, calretinin and S100G. (wikipedia.org)
  • In the brain, its synthesis is independent of vitamin-D. Calretinin, also known as calbindin 2, is a 29 kDa protein with 58% homology to calbindin 1 and principally found in nervous tissues. (wikipedia.org)
  • Calbindin 28K and calretinin are very similar calcium binding proteins which are both present in the central nervous system (CNS). (springer.com)
  • Two calcium binding proteins, calbindin and calretinin, have been reported to be expressed in abundance in Purkinje cells and other cell types in the cerebellum. (mybiosource.com)
  • Data show calbindin (CB)- and tyrosine hydroxylase (TH)-cells were distributed in the three striatal territories, and the density of calretinin (CR) and parvalbumin (PV) interneurons were more abundant in the associative and sensorimotor striatum. (nih.gov)
  • Compartmentalization of calcium binding proteins such as calretinin and calbindin-28 kDa has been noted within cells, suggesting that these proteins perform distinct functions in localized calcium signaling. (wikipedia.org)
  • Calcium binding proteins (CaBPs) such as calbindin D28-k, parvalbumin, and calretinin are able to bind Ca 2+ with high affinity. (hindawi.com)
  • We demonstrate that ΔFosB, a highly stable transcription factor, is induced in the hippocampus in mouse models of AD and seizures, in which it binds and triggers histone deacetylation at the promoter of the calbindin gene ( Calb1 ) and downregulates Calb1 transcription. (nature.com)
  • Rabbit IgG polyclonal antibody for Calbindin(CALB1) detection. (mybiosource.com)
  • Fig. 1: Formalin-fixed, paraffin-embedded human Kidney stained with Calbindin 1 Mouse Monoclonal Antibody (CALB1/3333). (abeomics.com)
  • The structure of rat calbindin was originally solved by nuclear magnetic resonance and was one of the largest proteins then to be determined by this technique. (wikipedia.org)
  • Unlike calbindin 1 and 2, S100G is a member of the S100 family of calcium-binding proteins. (wikipedia.org)
  • This paper describes the distribution of structures stained with mono- and polyclonal antibodies to the calcium-binding proteins calbindin D-28k and parvalbumin in the nervous system of adult rats. (nih.gov)
  • The burst of calcium release from the ER of muscle cells is buffered by the presence of calcium-binding proteins such as calbindin-D9k and parvalbumin. (thefreedictionary.com)
  • Uptake of 45 Ca induced into the matrix by 1,25(OH) 2 D 3 supports the hypothesis that the calcium-enriched matrix could trap calbindin-D proteins. (biochemj.org)
  • In previous investigations, the critical transcellular proteins, calbindin-D 9k (CaBP-9k) and -D 28k (CaBP-28k), were shown to affect other transcellular pathways by buffering intracellular calcium concentrations. (mdpi.com)
  • The work described in this thesis represents a biophysical approach, mainly using nuclear magnetic resonance (NMR) spectroscopy, to investigate the impact of magnesium ions on the EF-hand calcium-binding proteins calmodulin and calbindin D9k, as well as the dynamics of these proteins at low calcium levels. (dissertations.se)
  • There are currently no images for Calbindin D-28K Antibody (NBP1-35393). (novusbio.com)
  • Calbindin antibody is often used as a marker for cerebellum Purkinje cells. (novusbio.com)
  • Calbindin D staining of paraffin-embedded human cerebellum and frozen rat hypothalamus sections using 25 µg/mL mouse anti-human Calbindin D monoclonal antibody. (neuromics.com)
  • Staining is shown in the medial vestibular nucleus using 25 µg/mL mouse anti-human Calbindin D monoclonal antibody. (neuromics.com)
  • IHC analysis of Calbindin using anti-Calbindin antibody (MBS176994). (mybiosource.com)
  • The tissue section was then incubated with 1ug/ml rabbit anti-Calbindin Antibody (MBS176994) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. (mybiosource.com)
  • For adsorption we suggest the following procedure: - Dilute 1 µl of the monoclonal antibody 300 against calbindin D-28k in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5'000). (annoron.com)
  • antibody storage GENTAUR recommends for long therm storage to freeze at -24 C. For short time storage up to 30 days we suggest fridge storage at 1 to 10 C. Prevent multiple freeze taw cycles of Calbindin, monoclonal antibody, mouse, 100 ul. (antibody-antibodies.com)
  • Iacopino, A.M. & Christakos, S. Specific reduction of calcium-binding protein (28-kilodalton calbindin-D) gene expression in aging and neurodegenerative diseases. (nature.com)
  • Several animal studies have shown that BPA may disturb calcium metabolism by inducing or inhibiting the renal expression of a vitamin D-dependent calcium-binding protein, calbindin-D9k (CaBP-9k) (Kim et al. (thefreedictionary.com)
  • Downregulation of PCNA and calbindin-D9k protein levels after BPA exposure were confirmed by Western blot analysis (see Supplemental Material, Figure S1B,C). (thefreedictionary.com)
  • Studies in rats have shown that subcutaneous injection of p-NP in late pregnancy induces calbindin-D9k (CaBP-9k) mRNA and protein expression in maternal and neonatal uteri, suggesting its potential transfer through the placenta (Hong et al. (thefreedictionary.com)
  • Examples of differentially expressed representative genes include calbindin-D9k (vitamin D-dependent calcium-binding protein), oxytocin, adipocyte complement related protein (30 kDa), lactate dehydrogenase A, and calcium-binding protein A6 (calcyclin). (thefreedictionary.com)
  • Calbindin D-28K is a six-EF-hand calcium-binding protein found in the brain, peripheral nervous system, kidney, and intestine. (rti.org)
  • We investigated, by in situ hybridization histochemistry, the cellular localization of the mRNA encoding a vitamin D-dependent calcium-binding protein (calbindin D28) in rat brain and peripheral organs. (nih.gov)
  • Calbindin-D, also known as Calbindin 1, is a Vitamin D-dependent cytoplasmic protein in the EF-hand calcium binding protein family that includes calmodulin, parvalbumin, troponin C, and S100. (neuromics.com)
  • We show in gel-shift assays that the sequence within a putative vitamin-D-response element in the human calbindin-D9k promoter can bind expressed IPF-1/PDX-1 protein, although we cannot confirm binding of the vitamin-D-receptor protein. (biochemj.org)
  • Although Calbindin-D9k (CaBP-9k), a cytosolic calcium binding protein which has calcium binding sites, is expressed in various tissues, i.e., intestine, uterus, and placenta, potential roles of this gene and its protein are not clearly understood. (springer.com)
  • Downregulation of calbindin 1, a calcium-binding protein, reduces the proliferation of osteosarcoma cells. (ptglab.com)
  • E Coli-derived human Calbindin recombinant protein (Position: A2-E175). (mybiosource.com)
  • Calbindin is a calcium-binding protein belonging to the troponin C superfamily. (mybiosource.com)
  • CALB2 (Calbindin 2) is a Protein Coding gene. (genecards.org)
  • Prokaryotic recombinant protein corresponding to the majority of the calbindin molecule. (antibody-antibodies.com)
  • Both nonadapting (fast-spiking) and adapting (regular-spiking) inhibitory interneurons fired on thalamocortical stimulation, as did interneurons expressing parvalbumin, calbindin, or neither calcium-binding protein. (jneurosci.org)
  • The structure of calbindin is characteristic of an EF-hand protein, with two helix-loop-helix calcium binding motifs joined by a flexible linker, and a short anti-parallel beta-type interaction between the two ion-binding sites. (molmovdb.org)
  • Learning deficits in mice expressing familial AD-mutant hAPP correlated strongly with decreased levels of the calcium-binding protein calbindin-D 28k (CB) and the calcium-dependent immediate early gene product c-Fos in granule cells of the dentate gyrus, a brain region critically involved in learning and memory. (pnas.org)
  • A gene on chromosome Xp22.2 that encodes calbindin-D9K, a cytosolic vitamin D-dependent calcium-binding protein, which contains 2 active calcium-binding domains. (thefreedictionary.com)
  • It is encoded in humans by the CALB2 gene and was formerly known as calbindin-D29k. (wikipedia.org)
  • Figure 4: Increased hippocampal ΔFosB expression corresponds with decreased calbindin expression in human individuals diagnosed with MCI, AD, or TLE. (nature.com)
  • Whether decreased calbindin expression is unique to the hippocampal sclerosis associated with cryptogenic temporal lobe epilepsy, or also occurs in tumor- or malformation-related epilepsy, is unknown. (elsevier.com)
  • Degree and pattern of calbindin immunoreactivity in granule cells of the dentate gyrus differ in mesial temporal sclerosis, cortical malformation- and tumor-related epilepsies. (nature.com)
  • A loss of calbindin immunoreactivity in granule cells of the hippocampal dentate gyrus is a characteristic feature of temporal lobe epilepsy with hippocampal sclerosis. (elsevier.com)
  • We show that calbindin immunoreactivity in granule cells has been decreased in epilepsy regardless of its etiology. (elsevier.com)
  • Calbindin is a vitamin D-responsive gene in many tissues, in particular the chick intestine, where it has a clear function in mediating calcium absorption. (wikipedia.org)
  • Calbindin-D9K gene expression in rat chorioallantoic placenta is not regulated by 1,25-dihydroxyvitamin D3. (thefreedictionary.com)
  • Calbindin-D9K (CaBP9K) gene: a model for studying the genomic actions of cacitriol and calcium in mammals. (thefreedictionary.com)
  • Hwang I, Yang H, Kang H-S, Ahn C, Hong E-J, An B-S, Jeung E-B. Alteration of Tight Junction Gene Expression by Calcium- and Vitamin D-Deficient Diet in the Duodenum of Calbindin-Null Mice. (mdpi.com)
  • As a general characterization it can be stated that calbindin antibodies mainly label cells with thin, unmyelinated axons projecting in a diffuse manner. (nih.gov)
  • Using a [35S]cRNA probe under high stringency conditions, specific mRNA was found in tissues well known for their calbindin D28 content, e.g. renal distal tubules, cerebellar Purkinje cells and dentate gyrus granule cells. (nih.gov)
  • Correlation between calbindin expression in granule cells of the resected hippocampal dentate gyrus and verbal memory in temporal lobe epilepsy. (nih.gov)
  • In cases of cortical malformations or hippocampal sclerosis, calbindin immunoreactivity was undetectable in most granule cells. (elsevier.com)
  • In tumor-related resections, in patients who had a long history of epileptic seizures, calbindin was detected only in one-third of granule cells. (elsevier.com)
  • In contrast to tumor-induced epilepsy, where calbindin-immunoreactive granule cells were equally distributed in the granule cell layer, in hippocampal sclerosis and malformation-related epilepsy, two-thirds of calbindin-immunoreactive granule cells were located in the outer half and only one-third in the inner half of the layer. (elsevier.com)
  • The reduction of calbindin immunoreactivity in ontogenetically younger granule cells highlights the deleterious effect of early occurring epilepsy and initial early precipitating injury, including febrile seizures that may substantially affect developing immature granule cells, but less the earlier born matured ones. (elsevier.com)
  • Calbindin 1 or simply calbindin was first shown to be present in the intestine in birds and then found in the mammalian kidney. (wikipedia.org)
  • S100G, formerly calbindin 3 and calbindin-D9k, is present in mammalian enterocytes (epithelial cells of the intestine). (wikipedia.org)
  • one of these, known as IPF-1 or PDX-1, co-localizes in the intestine with calbindin-D9k. (biochemj.org)
  • Epithelial and neuronal calbindin in avian intestine: an immunohistochemical study. (springer.com)
  • the presence of calbindin increases the amount of calcium crossing the cell without raising the free concentration. (wikipedia.org)
  • We also describe the presence of calbindin in RINm5F cells, an insulin-producing tumor cell line derived from a radiationinduced rat insulinoma. (springer.com)
  • We have examined the cellular distribution of calbindin in the pancreatic endocrine cells of chick, rat and human and found variable distribution among the different endocrine cell types. (springer.com)
  • Calbindin-D9k (CaBP9k) localization and levels of expression in trophoblast cells from human term placenta. (thefreedictionary.com)
  • Calbindin D 27 kDa: preferential localization in non-B islet cells of the rat pancreas. (springer.com)
  • This study was carried out to investigate the morphology, distribution and co-localization of calbindin D-28k and parvalbumin-containing GABAergic cells in the midbrain of the cat. (koreamed.org)
  • 2. Parvalbumin immunoreactive cells in the red nucleus were more than twice in number compared to the calbindin D-28k immunoreactive cells. (koreamed.org)
  • 3. Calbindin immunoreactive cells in the substans nigr were more than twice in number compared to the parvalbumin immunoreactive cells. (koreamed.org)
  • 5. Calbindin D-28k and parvalbumin-immunoreactive cells were round, oval, spindle or polygonal in shape and were 15~20 micrometer in diameter. (koreamed.org)
  • Immunohistochemical analysis of paraffin-embedded human lymph node using Calbindin (D1I4Q) XP ® Rabbit mAb. (cellsignal.co.uk)
  • Stimulation of calbindin-D9k mRNA expression in the rat uterus by octyl-phenol, nonylphenol and bisphenol. (thefreedictionary.com)
  • Calbindin-D9k mRNA is tightly regulated during the estrous cycle in the rat uterus. (thefreedictionary.com)
  • These results suggest that human VNO epithelial cells of both genders express calbindin during development and in the adult. (semanticscholar.org)
  • Calbindin-like immunoreactivity in epithelial cells of the newborn and adult human vomeronasal organ. (semanticscholar.org)
  • The present study demonstrates that epithelial cells within the VNOs of both subjects expressed calbindin-like immunoreactivity. (semanticscholar.org)
  • BACKGROUND: Transient receptor potential channel type 6 (TRPV6) and Calbindin-D9k (CaBP-9k) are involved in the active calcium (Ca2+) transport mechanism in many tissues including placenta and uterus, suggesting a role in the establishment and maintenance of pregnancy. (uzh.ch)
  • Nonetheless, there is no homology between calbindin 1 and S100G, apart from their calcium binding domains (EF-hands): S100G has two EF-hands, and calbindin 1 has six. (wikipedia.org)
  • Expression of S100G, like that of calbindin 1, is stimulated by the active vitamin D metabolite, calcitriol although the precise mechanisms are still controversial. (wikipedia.org)
  • To further examine the mechanism and structural consequences of calcium binding to calbindin D-28K we performed detailed complementary heteronuclear NMR and microelectrospray mass spectrometry investigations of the calcium-induced conformational changes of calbindin D-28K. (rti.org)
  • Title: Downregulation of Calbindin 1 by miR-454-3p Suppresses Cell Proliferation in Nonsmall Cell Lung Cancer In Vitro. (nih.gov)
  • Calbindin D-28k is, however, also found in some short-axon cells (Golgi type II), represented by spinal cord interneurons in layer II and interneurons of the cerebral cortex. (nih.gov)
  • Regarding network development, a connectivity preference for cholecystokinin-expressing interneurons to target calbindin-expressing principal cells is diminished. (elifesciences.org)
  • In situ hybridization histochemistry allows the precise identification of cells expressing calbindin D28 and offers a new approach to study its regulation and possible role, e.g. in neuronal function. (nih.gov)
  • Here we present quantitative data on AHN in wild-derived mole-rats of 1 year and older, and briefly describe its anatomical context including markers of neuronal function (calbindin and parvalbumin). (frontiersin.org)
  • In 2018 the X-ray crystal structure of human calbindin was published (PDB entry 6FIE). (wikipedia.org)
  • Human, mouse, and rat Calbindin-D share 99% amino acid sequence identity. (neuromics.com)
  • We report studies of the human calbindin-D9k promoter. (biochemj.org)
  • Differences between the reported sequences of the human calbindin-D9k promoter were first clarified before undertaking a functional analysis of this sequence. (biochemj.org)
  • This indicates that other elements are also needed for the control of human calbindin-D9k expression. (biochemj.org)
  • Parmentier M, Lawson DEM, and Vassart G (1987) Human calbindin complementary DNA sequence. (springer.com)
  • Human Calbindin shares 99% amino acid (aa) sequence identity with mouse and rat Calbindin. (mybiosource.com)
  • Calbindin was detected in paraffin-embedded section of Human Intestinal Cancer Tissue. (mybiosource.com)
  • Tetrabromodiphenyl other (BDE-47) evokes estrogenicity and calbindin-D9k expression through an estrogen receptor-mediated pathway in the uterus of immature rats. (thefreedictionary.com)
  • Calbindin-D9k is expressed in the cytoplasm of intestinal cells, where it is critical for dietary calcium absorption. (biochemj.org)
  • Western blot analysis of extracts from various cell lines using Calbindin (D1I4Q) XP ® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). (cellsignal.co.uk)
  • Immunoprecipitation of calbindin from mouse brain extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or Calbindin (D1I4Q) XP ® Rabbit mAb (lane 3). (cellsignal.co.uk)
  • Western blot analysis was performed using Calbindin (D1I4Q) XP ® Rabbit mAb. (cellsignal.co.uk)
  • Immunohistochemical analysis of paraffin-embedded mouse kidney using Calbindin (D1I4Q) XP ® Rabbit mAb. (cellsignal.co.uk)
  • Confocal immunofluorescent analysis of normal rat brain using Calbindin (D1I4Q) XP ® Rabbit mAb (green) and GFAP (GA5) Mouse mAb #3670 (red). (cellsignal.co.uk)
  • Calbindin D-28k and parvalbumin immunoreactivity in the frontal cortex in patients with frontal lobe dementia of non-Alzheimer type associated with amyotrophic lateral sclerosis. (bmj.com)
  • The calbindin-D 28k was decreased in the putamen, the dorsal tier of the substantia nigra along the lower border of the red nucleus, and in the cerebellar cortex in multiple system atrophy. (nih.gov)
  • Most of the cells containing calbindin D-28k are vulnerable to neurodegenerative processes. (nih.gov)
  • Calbindin contains 4 active calcium-binding domains, and 2 modified domains that have lost their calcium-binding capacity. (wikipedia.org)
  • They were found to exist in two distinct sizes with a molecular weight of approximately 9 kDa and 28 kDa, and they were renamed calbindins. (wikipedia.org)
  • In the caudal ventral medulla, no TH-IR or PNMT-IR cells were calbindin-IR. (garvan.org.au)
  • A proportion of dendritic cells within the light zone of germinal centers are also noted to be positive for calbindin. (antibody-antibodies.com)
  • The arrow indicates the cell shown in ( F ). Analysis of GABA- ( B through E ) and calbindin- ( G through J ) expressing cells in slice cultures. (sciencemag.org)
  • Calbindin is present in cells resembling the tangentially oriented GABA-containing cells that are found in the IZ of the developing neocortex ( 7 , 11 ). (sciencemag.org)
  • GABA (Fig. 1 , B through E) or calbindin (Fig. 1 , G through J) immunofluorescence was present in about 20% of DiI-labeled neocortical cells ( 12 ). (sciencemag.org)
  • We identify calbindin-expressing principal cells which are mispositioned under cellular heterotopia. (elifesciences.org)
  • Ectopic calbindin-expressing principal cells develop relatively normal morphological features and stunted intrinsic physiological features. (elifesciences.org)