Calbindin 1: A calcium-binding protein that mediates calcium HOMEOSTASIS in KIDNEYS, BRAIN, and other tissues. It is found in well-defined populations of NEURONS and is involved in CALCIUM SIGNALING and NEURONAL PLASTICITY. It is regulated in some tissues by VITAMIN D.Calbindins: Calcium-binding proteins that are found in DISTAL KIDNEY TUBULES, INTESTINES, BRAIN, and other tissues where they bind, buffer and transport cytoplasmic calcium. Calbindins possess a variable number of EF-HAND MOTIFS which contain calcium-binding sites. Some isoforms are regulated by VITAMIN D.S100 Calcium Binding Protein G: A calbindin protein found in many mammalian tissues, including the UTERUS, PLACENTA, BONE, PITUITARY GLAND, and KIDNEYS. In intestinal ENTEROCYTES it mediates intracellular calcium transport from apical to basolateral membranes via calcium binding at two EF-HAND MOTIFS. Expression is regulated in some tissues by VITAMIN D.Calbindin 2: A calbindin protein that is differentially expressed in distinct populations of NEURONS throughout the vertebrate and invertebrate NERVOUS SYSTEM, and modulates intrinsic neuronal excitability and influences LONG-TERM POTENTIATION. It is also found in LUNG, TESTIS, OVARY, KIDNEY, and BREAST, and is expressed in many tumor types found in these tissues. It is often used as an immunohistochemical marker for MESOTHELIOMA.Parvalbumins: Low molecular weight, calcium binding muscle proteins. Their physiological function is possibly related to the contractile process.Astringents: Agents, usually topical, that cause the contraction of tissues for the control of bleeding or secretions.Calcium-Binding Proteins: Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS.Laryngeal Mucosa: The mucous lining of the LARYNX, consisting of various types of epithelial cells ranging from stratified squamous EPITHELIUM in the upper larynx to ciliated columnar epithelium in the rest of the larynx, mucous GOBLET CELLS, and glands containing both mucous and serous cells.Secretagogins: Secretagogins are EF HAND MOTIF-containing calcium-binding proteins that are involved in early neuronal migration and neurogenesis. They are also present in many adult organs and in brain and endocrine neoplasms.Interneurons: Most generally any NEURONS which are not motor or sensory. Interneurons may also refer to neurons whose AXONS remain within a particular brain region in contrast to projection neurons, which have axons projecting to other brain regions.EF Hand Motifs: Calcium-binding motifs composed of two helices (E and F) joined by a loop. Calcium is bound by the loop region. These motifs are found in many proteins that are regulated by calcium.Spinothalamic Tracts: A bundle of NERVE FIBERS connecting each posterior horn of the spinal cord to the opposite side of the THALAMUS, carrying information about pain, temperature, and touch. It is one of two major routes by which afferent spinal NERVE FIBERS carrying sensations of somaesthesis are transmitted to the THALAMUS.Neurons: The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.Immunohistochemistry: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.Calcium: A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.Nerve Tissue ProteinsCalcitriol: The physiologically active form of vitamin D. It is formed primarily in the kidney by enzymatic hydroxylation of 25-hydroxycholecalciferol (CALCIFEDIOL). Its production is stimulated by low blood calcium levels and parathyroid hormone. Calcitriol increases intestinal absorption of calcium and phosphorus, and in concert with parathyroid hormone increases bone resorption.Purkinje Cells: The output neurons of the cerebellar cortex.Ganglia, Sensory: Clusters of neurons in the somatic peripheral nervous system which contain the cell bodies of sensory nerve axons. Sensory ganglia may also have intrinsic interneurons and non-neuronal supporting cells.Cell Count: The number of CELLS of a specific kind, usually measured per unit volume or area of sample.Fetal Tissue Transplantation: Transference of fetal tissue between individuals of the same species or between individuals of different species.Thalamic Nuclei: Several groups of nuclei in the thalamus that serve as the major relay centers for sensory impulses in the brain.Choline O-Acetyltransferase: An enzyme that catalyzes the formation of acetylcholine from acetyl-CoA and choline. EC 2.3.1.6.Kidney Tubules, Distal: The portion of renal tubule that begins from the enlarged segment of the ascending limb of the LOOP OF HENLE. It reenters the KIDNEY CORTEX and forms the convoluted segments of the distal tubule.Chickens: Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.Retinal Horizontal Cells: NEURONS in the inner nuclear layer of the RETINA that synapse with both the RETINAL PHOTORECEPTOR CELLS and the RETINAL BIPOLAR CELLS, as well as other horizontal cells. The horizontal cells modulate the sensory signal.Solutions: The homogeneous mixtures formed by the mixing of a solid, liquid, or gaseous substance (solute) with a liquid (the solvent), from which the dissolved substances can be recovered by physical processes. (From Grant & Hackh's Chemical Dictionary, 5th ed)Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Nuclear Magnetic Resonance, Biomolecular: NMR spectroscopy on small- to medium-size biological macromolecules. This is often used for structural investigation of proteins and nucleic acids, and often involves more than one isotope.Allosteric Regulation: The modification of the reactivity of ENZYMES by the binding of effectors to sites (ALLOSTERIC SITES) on the enzymes other than the substrate BINDING SITES.Period Circadian Proteins: Circadian rhythm signaling proteins that influence circadian clock by interacting with other circadian regulatory proteins and transporting them into the CELL NUCLEUS.Cryptochromes: Flavoproteins that function as circadian rhythm signaling proteins in ANIMALS and as blue-light photoreceptors in PLANTS. They are structurally-related to DNA PHOTOLYASES and it is believed that both classes of proteins may have originated from an earlier protein that played a role in protecting primitive organisms from the cyclical exposure to UV LIGHT.GermanyFlavoproteinsPhotoreceptor Cells, Invertebrate: Specialized cells in the invertebrates that detect and transduce light. They are predominantly rhabdomeric with an array of photosensitive microvilli. Illumination depolarizes invertebrate photoreceptors by stimulating Na+ influx across the plasma membrane.Click Chemistry: Organic chemistry methodology that mimics the modular nature of various biosynthetic processes. It uses highly reliable and selective reactions designed to "click" i.e., rapidly join small modular units together in high yield, without offensive byproducts. In combination with COMBINATORIAL CHEMISTRY TECHNIQUES, it is used for the synthesis of new compounds and combinatorial libraries.Central Nervous System: The main information-processing organs of the nervous system, consisting of the brain, spinal cord, and meninges.Hemangioma: A vascular anomaly due to proliferation of BLOOD VESSELS that forms a tumor-like mass. The common types involve CAPILLARIES and VEINS. It can occur anywhere in the body but is most frequently noticed in the SKIN and SUBCUTANEOUS TISSUE. (from Stedman, 27th ed, 2000)Genitalia, Female: The female reproductive organs. The external organs include the VULVA; BARTHOLIN'S GLANDS; and CLITORIS. The internal organs include the VAGINA; UTERUS; OVARY; and FALLOPIAN TUBES.Endocrine Disruptors: Exogenous agents, synthetic and naturally occurring, which are capable of disrupting the functions of the ENDOCRINE SYSTEM including the maintenance of HOMEOSTASIS and the regulation of developmental processes. Endocrine disruptors are compounds that can mimic HORMONES, or enhance or block the binding of hormones to their receptors, or otherwise lead to activating or inhibiting the endocrine signaling pathways and hormone metabolism.Uterus: The hollow thick-walled muscular organ in the female PELVIS. It consists of the fundus (the body) which is the site of EMBRYO IMPLANTATION and FETAL DEVELOPMENT. Beyond the isthmus at the perineal end of fundus, is CERVIX UTERI (the neck) opening into VAGINA. Beyond the isthmi at the upper abdominal end of fundus, are the FALLOPIAN TUBES.Endocrine System: The system of glands that release their secretions (hormones) directly into the circulatory system. In addition to the ENDOCRINE GLANDS, included are the CHROMAFFIN SYSTEM and the NEUROSECRETORY SYSTEMS.Gossypium: A plant genus of the family MALVACEAE. It is the source of COTTON FIBER; COTTONSEED OIL, which is used for cooking, and GOSSYPOL. The economically important cotton crop is a major user of agricultural PESTICIDES.Ibotenic Acid: A neurotoxic isoxazole (similar to KAINIC ACID and MUSCIMOL) found in AMANITA mushrooms. It causes motor depression, ataxia, and changes in mood, perceptions and feelings, and is a potent excitatory amino acid agonist.Uncaria: A plant genus of the family RUBIACEAE. Members contain uncarine and other cytotoxic and hypotensive oxindole alkaloids.Hippocampus: A curved elevation of GRAY MATTER extending the entire length of the floor of the TEMPORAL HORN of the LATERAL VENTRICLE (see also TEMPORAL LOBE). The hippocampus proper, subiculum, and DENTATE GYRUS constitute the hippocampal formation. Sometimes authors include the ENTORHINAL CORTEX in the hippocampal formation.Cotton Fiber: A TEXTILE fiber obtained from the pappus (outside the SEEDS) of cotton plant (GOSSYPIUM). Inhalation of cotton fiber dust over a prolonged period can result in BYSSINOSIS.Excitatory Amino Acid Agonists: Drugs that bind to and activate excitatory amino acid receptors.Memory: Complex mental function having four distinct phases: (1) memorizing or learning, (2) retention, (3) recall, and (4) recognition. Clinically, it is usually subdivided into immediate, recent, and remote memory.Stainless Steel: Stainless steel. A steel containing Ni, Cr, or both. It does not tarnish on exposure and is used in corrosive environments. (Grant & Hack's Chemical Dictionary, 5th ed)Indigo Carmine: Indolesulfonic acid used as a dye in renal function testing for the detection of nitrates and chlorates, and in the testing of milk.Halothane: A nonflammable, halogenated, hydrocarbon anesthetic that provides relatively rapid induction with little or no excitement. Analgesia may not be adequate. NITROUS OXIDE is often given concomitantly. Because halothane may not produce sufficient muscle relaxation, supplemental neuromuscular blocking agents may be required. (From AMA Drug Evaluations Annual, 1994, p178)Microelectrodes: Electrodes with an extremely small tip, used in a voltage clamp or other apparatus to stimulate or record bioelectric potentials of single cells intracellularly or extracellularly. (Dorland, 28th ed)Stereotaxic Techniques: Techniques used mostly during brain surgery which use a system of three-dimensional coordinates to locate the site to be operated on.Electrodes: Electric conductors through which electric currents enter or leave a medium, whether it be an electrolytic solution, solid, molten mass, gas, or vacuum.Electrodes, Implanted: Surgically placed electric conductors through which ELECTRIC STIMULATION is delivered to or electrical activity is recorded from a specific point inside the body.

Characterization of nodular neuronal heterotopia in children. (1/362)

Neuronal heterotopia are seen in various pathologies and are associated with intractable epilepsy. We examined brain tissue from four children with subcortical or periventricular nodular heterotopia of different aetiologies: one with severe epilepsy following focal brain trauma at 17 weeks gestation, one with hemimegalencephaly and intractable epilepsy, one with focal cortical dysplasia and intractable epilepsy, and one dysmorphic term infant with associated hydrocephalus and polymicrogyria. The connectivity of nodules was investigated using histological and carbocyanine dye (DiI) tracing techniques. DiI crystal placement adjacent to heterotopic nodules revealed numerous DiI-labelled fibres within a 2-3 mm radius of the crystals. Although we observed labelled fibres closely surrounding nodules, the majority did not penetrate them. Placement of DiI crystals within nodules also identified a limited number of projections out of the nodules and in one case there was evidence for connectivity between adjacent nodules. The cellular and neurochemical composition of nodules was also examined using immunohistochemistry for calretinin and neuropeptide Y (NPY), which are normally expressed in GABAergic cortical interneurons. Within heterotopic nodules from all cases, numerous calretinin-positive neurons were identified, along with a few cell bodies and many processes positive for NPY. Calretinin-positive neurons within nodules were less morphologically complex than those in the cortex, which may reflect incomplete differentiation into an inhibitory neuronal phenotype. There were also abnormal clusters of calretinin-positive cells in the overlying cortical plate, indicating that the migratory defect which produces heterotopic nodules also affects development of the cortex itself. Thus, heterotopic nodules consisting of multiple neuronal cell types are associated with malformation in the overlying cortical plate, and have limited connectivity with other brain regions. This abnormal development of connectivity may affect neuronal maturation and consequently the balance of excitation and inhibition in neuronal circuits, leading to their epileptogenic potential.  (+info)

Measurements of [Ca2+] using fura-2 in glioma C6 cells expressing calretinin with GFP as a marker of transfection: no Ca2+-buffering provided by calretinin. (2/362)

Glioma C6 cells were transfected with a plasmid containing the calretinin (CR) and green fluorescent protein (GFP) coding regions to analyze the effect of CR's presence on [Ca2+]i. Positive transfectants were identified by the detection of GFP and [Ca2+]i was measured using fura-2 as a probe. We found that neither the basic [Ca2+]i nor activated [Ca2+]i achieved by exposure to ionomycin, ADP or thapsigargin were affected by CR's presence in transfected cells, despite the ability of CR to bind Ca2+ as part of fusion protein. The level of expressed CR was estimated as at least 1 microM. The presented results suggest that CR's function is unlikely to be an intracellular Ca2+-buffer and support the hypothesis that CR might be involved in a specific Ca2+-dependent process. The results of this work also show that the S65T mutant of GFP is compatible with fura-2 measurements of intracellular [Ca2+]. We have demonstrated that the presence of GFP, as a transfection marker of glioma C6 cells, does not disturb fura-2 fluorescence, the basal or activated [Ca2+]i in these cells.  (+info)

Immunohistological studies of metabotropic glutamate receptor subtype 6-deficient mice show no abnormality of retinal cell organization and ganglion cell maturation. (3/362)

Immature retinal ganglion cells (RGCs) initially show a multistratified dendritic pattern, and, during the postnatal period, these dendrites gradually monostratify into ON and OFF sublaminae. The selective agonist of group III metabotropic glutamate receptors (mGluR), L-2-amino-4-phosphonobutyrate (L-AP-4), hyperpolarizes ON bipolar cells and reduces glutamate release. On the basis of L-AP-4-evoked inhibitory effects on ON-OFF segregation of developing RGCs, it has been hypothesized that glutamate-mediated synaptic activity is crucial for formation of the ON-OFF network. Gene-targeted ablation of mGluR6 specifically expressed in ON bipolar cells blocks normal ON responses but has been predicted to enhance glutamate release from ON bipolar cells. The mGluR6 knock-out mouse therefore provides a unique opportunity to investigate whether glutamate release and ON responses are important factors in the development of ON-OFF segregation. The combination of several different morphological analyses indicates that ON bipolar cells, as well as several distinct amacrine cells, in mGluR6 knock-out mice are normally distributed and correctly extend their terminals to defined retinal laminae. Importantly, both alpha and delta RGCs in adult mGluR6 knock-out mice are found monostratified into cell type-specific layers. Furthermore, no difference between wild-type and mGluR6 knock-out mice is observed in the maturation and dendritic stratification of developing RGCs. Hence, despite a deficit in normal ON responses, mGluR6 deficiency causes no abnormality in the retinal cellular organization nor in the stratifications of both ON bipolar cells and developing and mature RGCs. Based on these findings, we discuss several possible mechanisms that may underlie ON-OFF segregation of RGCs.  (+info)

Impaired motor coordination and Purkinje cell excitability in mice lacking calretinin. (4/362)

In the cerebellum, the parallel fiber-Purkinje cell synapse can undergo long-term synaptic plasticity suggested to underlie motor learning and resulting from variations in intracellular calcium concentration ([Ca2+]i). Ca2+ binding proteins are enriched in the cerebellum, but their role in information processing is not clear. Here, we show that mice deficient in calretinin (Cr-/-) are impaired in tests of motor coordination. An impairment in Ca2+ homeostasis in Cr-/- Purkinje cells was supported by the high Ca2+-saturation of calbindin-D28k in these cells. The firing behavior of Purkinje cells is severely affected in Cr-/- alert mice, with alterations of simple spike firing rate, complex spike duration, and simple spike pause. In contrast, in slices, transmission at parallel fiber- or climbing fiber-Purkinje cell synapses is unaltered, indicating that marked modifications of the firing behavior in vivo can be undetectable in slice. Thus, these results show that calretinin plays a major role at the network level in cerebellar physiology.  (+info)

Postnatal expression of calretinin-immunoreactivity in periodontal Ruffini endings in the rat incisor: a comparison with protein gene product 9.5 (PGP 9.5)-immunoreactivity. (5/362)

The postnatal expression of immunoreactivity for calretinin, one of the calcium binding proteins, and for protein gene product 9.5 (PGP 9.5), a general neuronal marker, was investigated in mechanoreceptive Ruffini endings in the periodontal ligament of the rat incisor. Age-related changes in the expression of these two proteins in periodontal nerves were further quantified with a computerized image analysis. At 1 day after birth, a few PGP 9.5-immunoreactive nerve fibers and a still smaller number of calretinin-positive fibers were found in the periodontal ligament: they were thin and beaded in appearance and no specialized nerve terminals were recognized. Tree-like terminals, reminiscent of immature Ruffini endings, were recognizable in 4-day-old rats by PGP 9.5-immunohistochemistry, while calretinin-immunostaining failed to reveal these specialized endings. At postnatal 7-11 days when PGP 9.5-immunostaining could demonstrate typical Ruffini endings, calretinin-immunopositive nerve fibers merely tapered off without forming the Ruffini type endings. A small number of Ruffini endings showing calretinin-immunoreactivity began to occur in the periodontal ligament at 24-26 days after birth when the occlusion of the first molars had been established. At the functional occlusion stage (60-80 days after birth), the Ruffini endings showing calretinin-immunoreactivity drastically increased in number and density, but less so than those positive for PGP 9.5-immunoreaction. The delayed expression of calretinin suggests that the function of the periodontal Ruffini endings is established after the completion of terminal formation because Ca2+, which binds to calcium binding proteins including calretinin with high affinity, plays an important role in mechano-electric transduction.  (+info)

Calretinin immunoreactive nerve endings in the trachea and bronchi of the rat. (6/362)

Nerve endings showing calretinin immunoreactivity were examined in the lower respiratory tract of the adult rat. Tree-like nerve endings were immunostained in the tracheal and bronchial smooth muscle layer. The endings that arose from thick nerve fibers and formed corpuscles composed of many arborized nerve terminals. A few of the nerve endings were also observed in the lamina propria of the tracheal mucosa, close to the epithelial layer. Immunoelectron microscopy revealed that the immunoreactive terminals were filled with mitochondria and scattered among the intermuscular collagen fibrils. Schwann cell sheath and collagen fibrils were intercalated between the smooth muscle cells and nerve endings. The calretinin immunoreactive nerve endings observed in the present study seem to be slowly adapting stretch receptors.  (+info)

Colchicine causes excessive ocular growth and myopia in chicks. (7/362)

Colchicine has been reported to destroy ganglion cells (GCs) in the retina of hatchling chicks. We tested whether colchicine influences normal ocular growth and form-deprivation myopia, and whether it affects cells other than GCs. Colchicine greatly increased axial length, equatorial diameter, eye weight, and myopic refractive error, while reducing corneal curvature. Colchicine caused DNA fragmentation in many GCs and some amacrine cells and photoreceptors, ultimately leading to the destruction of most GCs and particular sub-sets of amacrine cells. Colchicine-induced ocular growth may result from the destruction of amacrine cells that normally suppress ocular growth, and corneal flattening may result from the destruction of GCs whose central pathway normally plays a role in shaping the cornea.  (+info)

Math1: an essential gene for the generation of inner ear hair cells. (8/362)

The mammalian inner ear contains the cochlea and vestibular organs, which are responsible for hearing and balance, respectively. The epithelia of these sensory organs contain hair cells that function as mechanoreceptors to transduce sound and head motion. The molecular mechanisms underlying hair cell development and differentiation are poorly understood. Math1, a mouse homolog of the Drosophila proneural gene atonal, is expressed in inner ear sensory epithelia. Embryonic Math1-null mice failed to generate cochlear and vestibular hair cells. This gene is thus required for the genesis of hair cells.  (+info)

In this study it was found that inhibitory CR cells do not co-expressed other neurochemical markers of the inhibitory interneurons such as galanin, nNOS, NPY and PV. This showed that CR represent another neurochemically distinct group of inhibitory interneurons in the dorsal horn. To investigate the synaptic connection between CR islet cells with CMrgD afferents, the third part of the study was carried out using the RorBCreERT2 mouse line with tamoxifen induction. In this study, it was found that intraperitoneal injection of tamoxifen in early stage of postnatal life resulted in more labelling of cells, meanwhile less cells were labelled if tamoxifen were given at the later stage of postnatal life. Surprisingly, under optimal conditions, only a quarter of the inhibitory CR cells in lamina II were immunoreactive for tdTomato. However, the majority of tdTomato cells in the SDH are inhibitory interneurons and immunoreactive for CR, thus showed that this mouse line was highly selective for ...
The superficial spinal dorsal horn contains a heterogeneous population of neurons that process sensory inputs. Information on the properties of excitatory interneurons in this region is limited. As calretinin is a protein thought to be restricted to an excitatory population in this region, the aim of this study was to characterize calretinin-expressing neurons. Most calretinin cells (85%) exhibited large A-type potassium currents and delayed firing action potential discharge, and received strong excitatory synaptic input, whereas the remainder exhibited hyperpolarization-activated cation currents and low threshold T-type calcium currents, and tonic- or initial bursting firing patterns, and received weak excitatory synaptic input. These respective features are consistent with properties of excitatory and inhibitory interneuron populations in this region of the spinal cord. Our findings have resolved a previously unidentified population of inhibitory interneurons. Furthermore, the contrasting ...
At least three CaBPs are abundant in various types of nerve cells : calbindin-D28, calretinin, and parvalbumin. The sequence of chick calretinin, from cDNA clones, is 60% homologous to that of chick...
... Research ready antibody. Applications: ICC; IF; IHC; WB. Species Reactivity: Bovine; Human; Mouse; Rat. Calretinin is expressed in mammalian central nerve system, testis, fallopian tube and pancreas. In the brain it is localized in certain classes of neurons, and antibodies to it are useful for identifying specific neuronal cell types.
There are no specific protocols for Recombinant Human Calretinin protein (ab100941). Please download our general protocols booklet
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Monoklonale und polyklonale Calretinin Antikörper für viele Methoden. Ausgesuchte Qualitäts-Hersteller für Calretinin Antikörper. Hier bestellen.
NMDA receptors on PV+ interneurons mediate supralinear integration at feedback synapses from local pyramidal neurons, enabling competing networks to lock onto salient inputs.
The long-term goal of our research program is to understand the neural circuit mechanisms underlying motivated behavior. The exquisite neural architecture of mi...
Inhibitory GABAergic interneurons are important for shaping patterns of activity in neocortical networks. We examined the distributions of inhibitory interneuron subtypes in layer II/III of areas V1 and V2 in 18 genera of anthropoid primates including New World monkeys, Old World monkeys, and hominoids (apes and humans). Interneuron subtypes were identified by immunohistochemical staining for calbindin, calretinin, and parvalbumin and densities were quantified using the optical disector method. In both V1 and V2, calbindin-immunoreactive neuron density decreased disproportionately with decreasing total neuronal density. Thus, V1 and V2 of hominoids were occupied by a smaller percentage of calbindin-immunoreactive interneurons compared to monkeys who have greater overall neuronal densities. At the transition from V1 to V2 across all individuals, we found a tendency for increased percentages of calbindin-immunoreactive multipolar cells and calretinin-immunoreactive interneurons. In addition, ...
TY - JOUR. T1 - Calretinin staining pattern aids in the differentiation of mesothelioma from adenocarcinoma in serous effusions. AU - Chhieng, David C.. AU - Yee, Herman. AU - Schaefer, Dawn. AU - Cangiarella, Joan F.. AU - Jagirdar, Jaishree. AU - Chiriboga, Luis A.. AU - Cohen, Jean Marc. PY - 2000/6/25. Y1 - 2000/6/25. N2 - BACKGROUND. The differentiation between malignant mesothelioma and adenocarcinoma based on morphology alone can be a diagnostic challenge. The majority of the available antibodies recognize molecules expressed by adenocarcinoma whereas to the authors knowledge specific markers for mesothelial cells are lacking. Calretinin, a calcium-binding protein, has been reported to be a selective marker for mesothelioma and largely is absent from adenocarcinoma on histologic material. The results with cytologic preparations have been inconsistent. METHODS. To evaluate the specificity of calretinin in differentiating mesothelioma from adenocarcinoma in cytologic preparations, 21 ...
It is clear that even mild forms of traumatic brain injury (TBI) can have lasting cognitive effects; however, the specific cellular changes responsible for the functional deficits remain poorly understood. Previous studies suggest that not all neurons respond in the same way and that changes to neuronal architecture may be subtype specific. The current study aimed to characterize the response of interneurons to TBI. To model TBI in vitro, the neurites of primary cortical neurons were transected at 15 days in vitro. In response, calretinin+ interneurons underwent significant neurite remodeling around the injury site. By examining the response of pyramidal neurons, GAD67-GFP+ interneurons, and calretinin+ interneurons to the injury, we found that this response was specific to the calretinin+ cells. To determine whether calretinin+ interneurons respond in this way to a clinically relevant in vivo model of mild diffuse and focal injury, we subjected mice to the lateral fluid percussion injury model. We
Effect of pre- and post-treatment with Bacopa monnieri (Brahmi) on phencyclidine-induced disruptions in object recognition memory and cerebral calbindin, parvalbumin, and calretinin immunoreactivity in rats P Piyabhan,1 P Tingpej,2 N Duansak11Division of Physiology, Department of Preclinical Science, Faculty of Medicine, Thammasat University, KlongLuang, Pathumthani, Thailand; 2Division of Microbiology, Department of Preclinical Science, Faculty of Medicine, Thammasat University, KlongLuang, Pathumthani, ThailandBackground: Decreased gamma-aminobutyric acid (GABA)-ergic neurons in the brain of both schizophrenic patients and animal models indicates that impairment of GABAergic function is implicated in pathophysiology of the disorder. Decreased GABAergic neurotransmission might be also involved in cognitive impairment, which is developed in schizophrenia. Brahmi (Bacopa monnieri) could be a new treatment and prevention for this cognitive deficit in schizophrenia by increasing GABAergic neurons to a
In the frog retina most bipolar cells, sparsely distributed amacrine cells and some ganglion cells contain calretinin (CaR). Double-label immunocytochemistry shows that in the Xenopus retina many calretinin positive amacrine cells are also gamma-aminobutyric acid (GABA)-immunoreactive (IR), none col …
One of the most ecologically-valid rodent models of depression is the learned helplessness paradigm. You get a rat or a mouse and you confine it in a cage with an electrified grid. Then you apply mild foot shocks at random intervals and of random duration for an hour (which is one session). The mouse initially…
Ong, W.Y.,He, Y.,Garey, L.J. (1997). Distribution of Amyloid β-protein Immunoreactivity in the Hippocampus of Rats Injected with Kainate. Journal of Brain Research 38 (3) : 353-361. [email protected] Repository ...
Each artery has three specific layers:. • The outer layer.. • The middle, muscular layer.. • The inner layer.. Each layer is made up of epithelial cells. The middle layer is elastic and very strong. It helps to pump the bodys blood. The inner layer is smoother and allows the blood to flow more easily.. As the heart beats, the arteries expand and get filled with blood. The heart then relaxes and produces enough force to push the blood through. In a healthy person, this system works effectively and the blood can carry oxygen and other essentials through the body.. Disease fills the arteries with fatty deposits, which becomes a dangerous obstacle to good health. High cholesterol levels fill arteries with thick, fatty substances that prevent your body from working properly. Your heart becomes starved of the required blood. When this happens you may suffer a heart attack or a stroke.. The main cause behind this heart disease is this thickening of the fatty deposits in the arteries, and the ...
mouse Mrgprd protein: Mrgprd(+) innervation is restricted to the epidermis and absent from specialized sensory structures. Furthermore, Mrgprd(+) fibers terminate in a specific layer of the epidermis, the stratum granulosum. RefSeq NM_203490
Skin can take quite a beating during the average lifetime. Its the bodys first defense against the outside world, so its no surprise that it pays a hefty price at the hands of age. Among factors such as smoking, UV exposure and the natural breakdown of proteins, the skin will begin to lose its natural elasticity over time. But why?. The first thing to know about the bodys largest organ is that the skin is composed of two specific layers: the epidermis and the dermis. While the epidermis protects your body against environmental hazards, the dermis is made up of the proteins and collagens responsible for skins elasticity. And while it is still unknown how these two layers interact over time or why exactly the skin is unable to "bounce back" from certain damages, scientists have several theories as to why the skin faces irreparable damage with age.. Medical News Today explores three major reasons why skin loses its youthful appearance.. First, the dermis is made up of extracellular matrix ...
A calcium-binding protein called calretinin may provide the basis for a new kind of blood test for the asbestos-linked cancer, malignant mesothelioma.
Lu S, Mukkada VA, Mangray S, Cleveland K, Shillingford N, et al (2012) MicroRNA profiling in mucosal biopsies of eosinophilic esophagitis patients pre and post glucocorticoid steroid treatment and relationship with mRNA target expression. PLoS One 7:e40676. Cao W, Tian W, Hong J, Li D, Tavares R, et al (2013) Expression of bile acid receptor TGR5 in gastric adenocarcinoma. Am J Phys Gast and Liver 304:G322-27.. Lu S, Singh K, Magray S, Tavares R, Nobel L, et al (2013) Claudin expression in high grade invasive ductal carcinoma of the breast. Mod Pathol 26:485-95.. Matoso A, Mukkada V, Lu S Monahan R, Cleveland K, Noble L, Mangray S, Resnick MB. (2013) Expression microarray analysis identifies novel epithelial derived proteins in eosinophilic esophagitis. Mod Pathol 26:665-672.. Taliano RJ, Lu S, Singh K, Mangray S, Tavares R, Nobel L, et al (2013) Calretinin expression in high grade invasive ductal carcinoma of the breast is associated with basal-like subtype and unfavorable prognosis. Human ...
Recombinant human Calbindin D9K was cloned from bovine cDNA and expressed inE.coli. It bounds to one Ca and one Ln ion. Two calcium ions are bound in the Calbindin D9K version. The protein consists of Calbindin D9K, mutant P47M (residues 5-79, swissprot a
Calbindin je termín označující skupinu transportních proteinů v buňkách tenkého střeva a v ledvinách. Tyto proteiny jsou závislé na funkci vitaminu D a umožňují absorpci vápníku ze střeva a ledvinných tubulů do organizmu ...
Supplies: Immunostain Technical Only Envelope (T693). Specimen Type: Tissue. Container/Tube: Immunostain Technical Only Envelope (T693). Preferred: 2 unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.. Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block. Additional Information:. 1. Information on accessing digital images of IHC stains and the manual requisition form can be accessed through this website: www.mayomedicallaboratories.com/test-info/ihc/index.html. 2. Clients ordering stains using a manual requisition form will not have access to digital images.. 3. Clients wishing to access digital images must place the order for IHC stains electronically. Information regarding digital imaging can be accessed through this website: www.mayomedicallaboratories.com/test-info/ihc/faq.html. ...
1BOC: The solution structures of mutant calbindin D9ks, as determined by NMR, show that the calcium-binding site can adopt different folds.
1IG5: Structural basis for the negative allostery between Ca(2+)- and Mg(2+)-binding in the intracellular Ca(2+)-receptor calbindin D9k.
Parvalbumin alpha / PVALB, 0.1 ml. Parvalbumin?is a?calcium-binding?albumin?protein with low molecular weight (typically 9-11 kDa).
Facts and data of a family history are often able to help in distinguishing between adenocarcinoma and epithelioid mesothelioma. When a patient possesses a family history of carcinoma, it is more likely that he or she suffers from a variant of the disease. When a patient has a history of asbestos exposure, it is more likely that he or she suffers from epithelioid mesothelioma. Or, if you or a loved one got asbestos exposure and received a diagnosis of adenocarcinoma, it may be worthwhile to look for a second diagnostic assessment, either by the same oncology specialist or a physician who has adequate experience in mesothelioma cases ...
Facts and data of a family history are often able to help in distinguishing between adenocarcinoma and epithelioid mesothelioma. When a patient possesses a family history of carcinoma, it is more likely that he or she suffers from a variant of the disease. When a patient has a history of asbestos exposure, it is more likely that he or she suffers from epithelioid mesothelioma. Or, if you or a loved one got asbestos exposure and received a diagnosis of adenocarcinoma, it may be worthwhile to look for a second diagnostic assessment, either by the same oncology specialist or a physician who has adequate experience in mesothelioma cases ...
& 2004 USCAP, Inc All rights reserved /04 $ immunostaining in distinguishing epithelial mesothelioma and lung adenocarcinoma Karine Llinares 1,2, Fabienne Escande
To investigate the electrophysiological properties, synaptic connections, and anatomy of individual parvalbumin-immunoreactive (PV-IR) and cholecystokinin-immunoreactive (CCK-IR) interneurones in CA1, dual intracellular recordings using biocytin-filled microelectrodes in slices of adult rat hippocampus were combined with fluorescence labelling of PV- and CCK-containing cells. Of 36 PV-IR cells, 29 were basket cells, with most of their axonal arbours in the stratum pyramidale (SP). Six were bistratified cells with axons ramifying throughout stratum oriens (SO) and stratum radiatum (SR). One was a putative axo-axonic cell with an axonal arbour confined to half of the SP and a narrow adjacent region of the SO. Of 27 CCK-IR neurones, 13 were basket cells, with most of their axonal arbours in the SP, and included basket cells with somata in the SP (6), SO (3), and SR (2) and at the border between the stratum lacunosum-moleculare (SLM) and the SR (2). In addition, several dendrite-targeting cell ...
Gentaur molecular products has all kinds of products like :search , Neuromi \ Calbindin, monoclonal antibody, mouse, 100 ul... \ MO20016-100 for more molecular products just contact us
The claustrum is present in all mammalian species examined so far and its morphology, chemoarchitecture, physiology, phylogenesis and ontogenesis are still a matter of debate. Several morphologically distinct types of immunostained cells were described in different mammalian species. To date, a comparative study on the neurochemical organization of the human and non-human primates claustrum has not been fully described yet, partially due to technical reasons linked to the postmortem sampling interval. The present study analyzes the localization and morphology of neurons expressing parvalbumin (PV), calretinin (CR), NPY, and somatostatin (SOM) in the claustrum of man (# 5), chimpanzee (# 1) and crab-eating monkey (#3). Immunoreactivity for the used markers was observed in neuronal cell bodies and processes distributed throughout the anterior-posterior extent of human, chimpanzee and macaque claustrum. Both CR- and PV-immunoreactive (ir) neurons were mostly localized in the central and ventral region of
The differentiation between adenocarcinoma and mesothelioma can be difficult at histology. In this case, the initial histological diagnosis of metastatic adenocarcinoma was revised following review of clinical and radiologal features, and the cor...
Top performende anti-Human S100 Calcium Binding Protein P Antikörper für Immunofluorescence (fixed cells) (IF/ICC) vergleichen & kaufen.
Reagents for the antigen Basalin / S100 calcium binding protein A17 / S100A17 / trichohyalin-like 1 / TCHHL1 / THHL1 stained with Cy5™ in the Antibody Database
Calcium binding protein information including symptoms, causes, diseases, symptoms, treatments, and other medical and health issues.
DAB staining of tissue with this antibody showed high specificity and very low background at a concentration of 1:2000. For fluorescence staining of tissue. the optimal concentration was 1:1000.. This was also tested on neurons in culture where a clear difference between Calbindin-positive and -negative was seen at a concentration of 1:1000.. This was not tested for IP or WB.. ...
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CALR antibody - C-terminal region (ARP30114_P050) | Application: WB | CALR is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells | Alias: RO; CRT; SSA; cC1qR
pep:known chromosome:VEGA66:3:90511034:90514392:-1 gene:OTTMUSG00000022107 transcript:OTTMUST00000052482 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:S100a1 description:S100 calcium binding protein A1 ...
Hey all you histonetters! Could some tell me a good FLI 1C19, BCL1, Uroplakins, Hepapari, Calretinin and Cytokeratin 5 antibody for formalin-fixed paraffin embedded tissues. Thanks in advance! Muhammad Tahseen Histology Supervisor ...
A new monoclonal antibody, Ber-EP4, directed against a partially formol resistant epitope on the protein moiety of two 34 kilodalton and 39 kilodalton glycopolypeptides on human epithelial cells is described. Immunostaining of a wide range of normal and neoplastic human tissues and cell lines showed that all carcinomas and all non-neoplastic epithelial cells, except hepatocytes, parietal cells, and apical cell layers in squamous epithelia, homogeneously expressed Ber-EP4 antigen. As Ber-EP4 does not detect any normal or neoplastic non-epithelial cells, this antibody might prove valuable for the differentiation of the following (i) non-epithelial tumours from undifferentiated carcinomas; (ii) hepatocytes from bile duct cells in certain liver diseases; (iii) mesothelial cells from carcinoma cells in lung biopsy specimens; and (iv) reactive mesothelial cells from carcinoma cells in smears of serous effusions.. ...
Worldwide expansion of mobile phones and electromagnetic field (EMF) exposure has raised question of their possible biological effects on the brain and nervous system. Radiofrequency (RF) radiation might alter intracellular signaling pathways through changes in calcium (Ca(2+)) permeability across cell membranes. Changes in the expression of calcium binding proteins (CaBP) like calbindin D28-k (CB) and calretinin (CR) could indicate impaired Ca(2+)homeostasis due to EMF exposure. CB and CR expression were measured with immunohistochemistry in the hippocampus of mice after EMF exposure at 835 MHz for different exposure times and absorption rates, 1 h/day for 5 days at a specific absorption rate (SAR)=1.6 W/kg, 1 h/day for 5 days at SAR=4.0 W/kg, 5 h/day for 1 day at SAR=1.6 W/kg, 5 h/day for 1 day at SAR=4.0 W/kg, daily exposure for 1 month at SAR=1.6 W/kg. Body weights did not change significantly. CB immunoreactivity (IR) displayed moderate staining of cells in the cornu ammonis (CA) areas and ...
The progenitors of cerebellar GABAergic interneurons proliferate up to postnatal development in the prospective white matter, where they give rise to different neuronal subtypes, in defined quantities and according to precise spatiotemporal sequences. To investigate the mechanisms that regulate the specification of distinct interneuron phenotypes, we examined mice lacking the G1 phase-active cyclin D2. It has been reported that these mice show severe reduction of stellate cells, the last generated interneuron subtype. We found that loss of cyclin D2 actually impairs the whole process of interneuron genesis. In the mutant cerebella, progenitors of the prospective white matter show reduced proliferation rates and enhanced tendency to leave the cycle, whereas young postmitotic interneurons undergo severe delay of their maturation and migration. As a consequence, the progenitor pool is precociously exhausted and the number of interneurons is significantly reduced, although molecular layer ...
Unipolar brush cell: | | | |Unipolar brush cell| | | | | ... World Heritage Encyclopedia, the aggregation of the largest online encyclopedias available, and the most definitive collection ever assembled.
Looking for online definition of S100 calcium binding protein A3 in the Medical Dictionary? S100 calcium binding protein A3 explanation free. What is S100 calcium binding protein A3? Meaning of S100 calcium binding protein A3 medical term. What does S100 calcium binding protein A3 mean?
Buy our Recombinant Human S100 calcium binding protein A14. Ab101041 is a full length protein produced in Escherichia coli and has been validated in WB…
... , Authors: M Rosario Fernandez-Fernandez, Alan R Fersht. Published in: Atlas Genet Cytogenet Oncol Haematol.
The protein encoded by this gene is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. Originally described as a 27 kDa protein, it is now known to be a 28 kDa protein. It contains four active calcium-binding domains, and has two modified domains that are thought to have lost their calcium binding capability. This protein is thought to buffer entry of calcium upon stimulation of glutamate receptors. Depletion of this protein was noted in patients with Huntington disease. [provided by RefSeq, Jan 2015 ...
Rabbit Polyclonal Anti-Calbindin D-28K Antibody - Pre-diluted. Validated: IHC, IHC-P. Tested Reactivity: Human, Mouse, Rat. 100% Guaranteed.
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p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Recombinant protein of human S100 calcium binding protein A11 (S100A11), 20 ug available for purchase from OriGene - Your Gene Company.
Holtmaat, A., Bonhoeffer, T., Chow, D.K., Chuckowree, J., De Paola, V., Hofer, S.B., Hübener, M., Keck, T., Knott, G., Lee, W.C., Mostany, R., Mrsic-Flogel, T.D., Nedivi, E., Portera-Cailliau, C., Svoboda, K., Trachtenberg, J.T., Wilbrecht, L. Long-term, high-resolution imaging in the mouse neocortex through a chronic cranial window. Nat Protocol 4(8), 1128-1144 (2009). Epub 2009 Jul 16. PDF. ...
en] BACKGROUND: Uterine tumors resembling ovarian sex cord tumors (UTROSCTs) are rare neoplasms thought to be of putative endometrial stromal origin and solely composed of sex cord elements. Our study aimed to delineate the immunophenotype of these tumors and to verify whether their morphology reflects true sex cord-like differentiation. DESIGN: Representative paraffin blocks from 12 UTROSCTs were selected after confirmation of the diagnosis. Cords and/or trabeculae were seen in all tumors, whereas tubules, diffuse areas, and a retiform pattern were present in 9, 6, and 2 cases, respectively. Tumors were stained for sex cord (inhibin, calretinin, WT1, and melan-A), epithelial (KL1 and epithelial membrane antigen), and smooth muscle markers (smooth muscle actin, desmin, smooth muscle myosin heavy chain, h-caldesmon, and histone deacetylase-8), CD10, HMB45, S100, and CD117. Intensity and percentage of staining were recorded. RESULTS: Six out of 12 tumors were positive for sex cord markers (inhibin ...
Looking for online definition of S100 calcium-binding protein A13 in the Medical Dictionary? S100 calcium-binding protein A13 explanation free. What is S100 calcium-binding protein A13? Meaning of S100 calcium-binding protein A13 medical term. What does S100 calcium-binding protein A13 mean?
Neocortical neurones can be classified according to several independent criteria: morphological, physiological, and molecular expression (neuropeptides (NPs) and/or calcium-binding proteins (CaBPs)). While it has been suggested that particular NPs and CaBPs characterize certain anatomical subtypes of neurones, there is also considerable overlap in their expression, and little is known about simultaneous expression of multiple NPs and CaBPs in morphologically characterized neocortical neurones. Here we determined the gene expression profiles of calbindin (CB), parvalbumin (PV), calretinin (CR), neuropeptide Y (NPY), vasoactive intestinal peptide (VIP), somatostatin (SOM) and cholecystokinin (CCK) in 268 morphologically identified neurones located in layers 2-6 in the juvenile rat somatosensory neocortex. We used patch-clamp electrodes to label neurones with biocytin and harvest the cytoplasm to perform single-cell RT-multiplex PCR. Quality threshold clustering, an unsupervised algorithm that clustered
This test includes only technical performance of the stain (no pathologist interpretation is performed). Mayo Clinic cannot provide an interpretation of tech only stains outside the context of a pathology consultation. If an interpretation is needed, refer to test code PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case. All material associated with the case is required. Additional specific stains may be requested as part of the pathology consultation, and will be performed as necessary at the discretion of the Mayo pathologist.. The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.. Interpretation of this test should be performed in the context of the patients clinical history and other diagnostic tests by a ...
The encoded protein is a secreted calcium-binding protein which is found in the cytoplasm. It is related to calbindin D-28K and calretinin. This protein is thought to be involved in potassium chloride-stimulated calcium flux and cell proliferation.[6] This protein plays an important role in the release of the stress hormone Corticotropin-releasing hormone (CRH) and which only then enables stress processes in the brain. ...
Stratification of cancer patients for susceptibility to therapy with PTK2 inhibitors - The present invention relates to a method for determining whether a cancer patient is susceptible to treatment with a protein tyrosine kinase 2 (PTK2) inhibitor, comprising detecting the expression of the E-cadherin protein in a cancer sample of said cancer patient, wherein an E-cadherin protein immunoreactivity score (IRS) of 0-2 indicates that the cancer patient is susceptible to treatment with a PTK2 inhibitor. Said detection of the expression of the E-cadherin protein in a cancer sample of a cancer patient is preferably conducted by way of an immunohistochemistry (IHC) method. Said IHC method preferably employs a primary antibody which is specific for E-cadherin and a secondary antibody which specifically reacts with the primary antibody. The present invention also relates to a method of treating a cancer patient whose cancer is characterized by an E-cadherin protein immunoreactivity score (IRS) of 0-2, ...
Shortall, S. E., Brown, A. M., Newton-Mann, E., Dawe-Lane, E., Evans, C., Fowler, M., & King, M. V. (2020). Calbindin Deficits May Underlie Dissociable Effects of 5-HT6 and mGlu7 Antagonists on Glutamate and Cognition in a Dual-Hit Neurodevelopmental Model for Schizophrenia. Molecular Neurobiology, https://doi.org/10.1007/s12035-020-01938-x © 2020, The Author(s). Despite several compounds entering clinical trials for the negative and cognitive symptoms of schizophrenia, few have progressed beyond phase III. This is partly attributed to a need for improved preclinical models, to understa... Read More about Calbindin Deficits May Underlie Dissociable Effects of 5-HT6 and mGlu7 Antagonists on Glutamate and Cognition in a Dual-Hit Neurodevelopmental Model for Schizophrenia. ...
casSAR Dugability of Q9DB16 | Cab39l | Calcium-binding protein 39-like - Also known as CB39L_MOUSE, Cab39l. Component of a complex that binds and activates STK11/LKB1. In the complex, required to stabilize the interaction between CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta) and STK11/LKB1 (By similarity). Component of a trimeric complex composed of STK11/LKB1, STRAD (STRADA or STRADB) and CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta): the complex tethers STK11/LKB1 in the cytoplasm and stimulates its catalytic activity.
A 53-year-old man presented with a large soft tissue mass in the chest wall. Tumor infiltration into the lung was demonstrated on CT scan. The resected tumor (16 x 14 x 8 cm) was received with a portion of lung. The cut surface showed a poorly demarcated tan-pink tumor with focal areas of necrosis and multifocal extension to surgical margins. Tumor cells were CD34(+), bcl-2(+), CD99(+) and focally smooth muscle actin(+) and were CD31(-), desmin(-), calretinin(-) and cytokeratin(-).. The master list with the correct ...
The idea of Professors Bolis and Gilles to gather together for a 3 days meeting in the splendid environment of Crans-Montana in Switzerland a limited number of people around the subject of calcium an
Parvalbumin in human brain.: Parvalbumin was isolated from human cerebral cortex and biceps and triceps muscles by HPLC. The immunological properties of the hum
It takes a village of diverse neurons to make a brain, and among the villagers, parvalbumin (PV)-containing interneurons hold a special position. Here, SRF reviews recent developments in PV interneuron biology, including two studies specific to schizophrenia, which bolster the proposition that PV interneurons are structurally sound but functionally compromised in the disorder. Read this story…
S100A12 - S100A12 (untagged)-Human S100 calcium binding protein A12 (S100A12) available for purchase from OriGene - Your Gene Company.
100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use ...
The prefrontal cortex (PFC) dopamine system, which is critical for modulating PFC function, undergoes remodeling until at least young adulthood in primates. Catechol-o-methyltransferase (COMT) alters extracellular dopamine levels in PFC, and its gene contains a functional polymorphism (Val(158)Met) that has been associated with variation in PFC function. We examined COMT enzyme activity and protein immunoreactivity in the PFC during human postnatal development. Protein was extracted from PFC of normal individuals from 6 age groups: neonates (1-4 months), infants (5-11 months), teens (14-18 years), young adults (20-24 years), adults (31-43 years), and aged individuals (68-86 years; n = 5-8 per group). There was a significant 2-fold increase in COMT enzyme activity from neonate to adulthood, paralleled by increases in COMT protein immunoreactivity. Furthermore, COMT protein immunoreactivity was related to Val(158)Met genotype, as has been previously demonstrated. The significant increase in COMT activity
Results Seventeen of the mesotheliomas (four PMM from women) were classified as diffuse, while five were localised. Calretinin was 91% positive in PMM, but negative in PPSC (specificity, 100%). BerEP4, Claudin-4 and PAX8 were all 100% positive in PPSC (specificities, 100%, 95% and 95%, respectively, for excluding PMM). For distinguishing PMM and RMH, sensitivity for EMA in mesothelioma was 68%, while for IMP3 and GLUT-1 it was 64% and 50%, respectively, all with high specificities. FISH analysis revealed homozygous deletion of the 9p21 locus in 11/13 PMMs, but in 0/11 RMHs. ...
This gene encodes a nucleic-acid binding protein with seven zinc-finger domains. The protein has a preference for binding single stranded DNA and RNA…
This is a model network of prefrontal cortical microcircuit based primarily on rodent data. It includes 16 pyramidal model neurons, 2 fast spiking interneuron models, 1 regular spiking interneuron model and 1 irregular spiking interneuron model. The goal of the paper was to use this model network to determine the role of specific interneuron subtypes in persistent activity ...
OBJECTIVE: To analyze the role of immunochemistry in serous effusions.STUDY DESIGN: We analyzed cell blocks of 18 pleural and 18 peritoneal effusions diagnosed as malignant (18), benign (14) and suspicious (4). They were immunostained by the avidin-biotin complex method with a panel of four monoclonal antibodies-CEA, Ber-EP4, LeuM1 (CD15) and r53-and for lectins (Ulex europaeus) UEA-1, ConA and ConBr. RESULTS: Seventeen of the 18 cases of adenocarcinoma were positive for CEA (95%), 12 (66.6%)for Bev-EP4, 11 (61 %)for CD15 nild 11 (62 %)for p53. Twelve of the 18 (66.6%) were positive for UEA-1, CEA, Ber-EP4 and CD15. UEA-1 did not react with mesothelial cells. p53 Gave a positive reaction in only one case, reactive mesothelial cells. ConA and ConBr reacted indiscriminately with benign and malignant cells; thus, it was not useful in distinguishing between these cells.CONCLUSION: In this context no antibody used alone is reliable for corroborating a diagnosis, but the selective use of a small panel ...
S100 Calcium Binding Protein B (S100B) is an acidic calcium binding protein and a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and
Ptac2S is more effective than Platinol (cisplatin) at reducing the growth rate of sarcomatoid malignant pleural mesothelioma in mice, according to a study.. The finding comes on top of a study showing that PtacsS reduced the growth of epithelioid mesothelioma in mice. PtacsSs scientific designation is Pt(O,O′-acac)(γ-acac)(DMS).. The latest research, "Apoptosis by [Pt(O,O′-acac)(γ-acac)(DMS)] requires PKC-δ mediated p53 activation in malignant pleural mesothelioma," was published in the journal PLOS,One.. At the moment the mesothelioma treatment that does the best job of lengthening patients lives is a combination of Platinol (cisplatin) and either Alimta (pemetrexed) or Tomudex (raltitrexed). But survival rates are still low, with only half of patients living longer than 12 months after treatment.. Adding biological agents that target cancer-linked proteins to standard chemotherapy has had little impact on mesothelioma, highlighting the urgent need for new drugs.. There are three ...
There is new evidence that a blood test for a biomarker called N-ERC/mesothelin could help identify mesothelioma earlier and improve outcomes in people at risk for the disease.. Cancer researchers with Juntendo University in Tokyo released a study in 2008 suggesting that N-ERC/mesothelin, a soluble protein released by certain kinds of cancer cells, was "a very promising tumor marker for mesothelioma, especially epithelioid mesothelioma". In their newest study, the same group attempted to test the value of the biomarker as a warning tool for closer monitoring in populations at high mesothelioma risk.. The new study included construction workers who were part of a large-scale, 5-year screening of people known to be exposed to asbestos, the primary cause of mesothelioma. The screening program, which started in 2007, collected yearly blood samples from 40,000 construction workers at risk for mesothelioma, and evaluated their levels of N-ERC/mesothelin.. Based on the results of those tests, along ...
All patients with pleural mesothelioma is diagnosed should get some kind of treatment that can extend the useful life and the survival of the State-of-the-art treatments that are available, although it is not a cure for pleural mesothelioma, as is done with most of the cancers. Treatment of pleural mesothelioma include surgery, chemotherapy, and for the radius of the care and treatment option depends on the number, such as pleural mesothelioma has spread to other organs, at the age of the patient and the patients general health. ...
The present investigation has demonstrated that ionizing radiation in the therapeutic dose range stimulates a transient cellular generation of ROS/RNS. Temporally coincident is a radiation-induced reversible depolarization of the mitochondrial ΔΨ and decrease in mitochondrial entrapped calcein fluorescence, both hallmarks of the mitochondrial permeability transition. The amount of ROS/RNS generated is relatively constant over the dose range tested, but the number of cells that respond increases with the dose. The radiation-induced ROS/RNS generation, ΔΨ depolarization, and calcein release are inhibited by CsA but not by the structural analogue, CsH. Overexpression of the Ca2+-binding protein calbindin 28K or treatment of cells with BAPTA/AM, an intracellular Ca2+ chelator, also effectively block radiation-induced ROS/RNS. The increased ROS/RNS generation observed with radiation is common to all of the cell types examined but for one important exception, the mitochondrial DNA-less ρo ...
The vomeronasal organs (VNOs) of two humans, a male neonate and a female adult, were examined for immunolocalization of calbindin-D28k (calbindin) which has been immunolocalized to VNO receptor cells in other mammals. The present study demonstrates that epithelial cells within the VNOs of both subjects expressed calbindin-like immunoreactivity. These results suggest that human VNO epithelial cells of both genders express calbindin during development and in the adult.
By Lee M. Krug, MD. Malignant pleural mesothelioma (MPM) is a rare disease, with an incidence 100-fold less common than lung cancer. As such, the optimal treatment for a patient with MPM is not always clear-cut, and management can vary greatly from one institution to another. Large prospective studies do not exist to guide even our most basic treatment decisions, such as when to perform surgery. If possible, patients should seek care from a specialized center where the treating physicians can rely on their experience as well as the available literature to personalize an appropriate course of therapy.. How do I work up a patient with suspected mesothelioma? Most patients will present with a pleural effusion, but cytology from a thoracentesis is almost always non-diagnostic. Thus, a more invasive procedure such as video thoracoscopy is generally required to provide adequate tissue for analysis. Epithelioid mesothelioma can be difficult to distinguish histologically from adenocarcinoma, but ...
Calcium-Binding Proteins: Proteins to which calcium ions are bound. They can act as transport proteins, regulator proteins, or activator proteins. They typically contain EF HAND MOTIFS.
EMMX proudly develops novel molecules and reagents to drug discovery and cancer research. We are scientists driven by a devotion to discovery and innovation, and we work hard every day to find new ways to streamline life science research.. ...
Treatment for pleural mesothelioma This information is an extract from the booklet Understanding mesothelioma. You may find the full booklet helpful. We can send you a free copy see page 9. Contents Treatment
CAB39 antibody (calcium binding protein 39) for ICC/IF, IHC-P, WB. Anti-CAB39 pAb (GTX110628) is tested in Human, Mouse samples. 100% Ab-Assurance.
The two major goals of this project is to 1) provide the research community and public domain for the first time with a comprehensive genome-wide atlas of the h...
Vi stryker inl gg som anv nder ett oacceptabelt spr kbruk - till exempel svordomar eller kr nkande omd men om andra debatt rer. Vi f rbeh ller oss ocks r tten att stryka inl gg som avl gsnar sig alltf r l ngt fr n anslagstavlans avsedda anv ndningsomr de ...
This gene encodes calbindin D9K, a vitamin D-dependent calcium-binding protein. This cytosolic protein belongs to a family of ... Jeung EB, Krisinger J, Dann JL, Leung PC (Sep 1992). "Molecular cloning of the full-length cDNA encoding the human calbindin- ... 2004). "Control of differentiation-induced calbindin-D9k gene expression in Caco-2 cells by cdx-2 and HNF-1α". Am. J. Physiol. ... "Entrez Gene: S100G S100 calcium binding protein G". Balmain N (1991). "Calbindin-D9k. A vitamin-D-dependent, calcium-binding ...
"Entrez Gene: calbindin 2". Parmentier M, Passage E, Vassart G, Mattei MG (1991). "The human calbindin D28k (CALB1) and ... Calretinin also known as 29 kDa calbindin is a calcium-binding protein involved in calcium signaling. In humans, the calretinin ... Expression was found in different neurons than that of the similar vitamin D-dependent calcium-binding protein, calbindin-28kDa ... 16 (2): 118-24. doi:10.1097/PAP.0b013e31819923ce. PMID 19550372. Winn B, Tavares R, Fanion J, Noble L, Gao J, Sabo E, Resnick ...
Cited over 1500 times Celio MR (1990). "Calbindin D-28k and parvalbumin in the rat nervous system". Neuroscience. 35 (2): 375- ...
IMPA1 has been shown to interact with Bergmann glial S100B and calbindin. L-690,330 is a competitive inhibitor of IMPase ... Schmidt H, Schwaller B, Eilers J (April 2005). "Calbindin D28k targets myo-inositol monophosphatase in spines and dendrites of ... Berggard T, Szczepankiewicz O, Thulin E, Linse S (2003). "Myo-inositol monophosphatase is an activated target of calbindin D28k ... "Myo-inositol monophosphatase is an activated target of calbindin D28k". J. Biol. Chem. 277 (44): 41954-9. doi:10.1074/jbc. ...
The latter group is represented by calbindin D9k and do not undergo calcium dependent conformational changes. EPS15 homology ( ... and Ca2+ buffers such as calreticulin and calbindin D9k. While the majority of the known EF-hand Calcium-binding proteins ( ... 269 (2): 454-6. doi:10.1016/0014-5793(90)81214-9. PMID 2401372. Finn BE, Forsén S (January 1995). "The evolving model of ... 11 (2): 198-205. doi:10.1110/ps.33302. PMC 2373453 . PMID 11790829. Eukaryotic Linear Motif resource motif class LIG_EH_1 ...
Vitamin D also increases transcription of genes that code for Calbindin. As the name implies, Calbindin functions as a calcium- ... Reddy and Katan recommend a salt intake below 5 g/day (5000 mg/day), and to increase potassium intake by 2 - 3 g/day. The study ... 2)Releases calcium from bones into the blood Vitamin D also plays a crucial role in maintaining serum calcium levels (Ca++ in ...
Choline deficiency alters global histone methylation and epigenetic marking at the Re1 site of the calbindin 1 gene. FASEB J. ... 13(2):115-7 doi:10.1016/j.cmet.2011.01.011 PMID 21284975 Ng, S.F., Lin, R.C., Laybutt, D.R., Barres, R., Owens, J.A., and ... 2007 Feb;65(2):88-94 PMID 17345961 Martin JF, Johnston CR, Han CT, Benyshek DC. Nutritional origins of insulin resistance: a ... 2010 Mar;77(2):225-35 doi:10.1002/msj.20176 PMID 20309920. Gheorghe CP, Goyal R, Holweger JD, Longo LD. Placental gene ...
Other calcium-binding protein markers are calretinin (most abundant subtype in DLPFC, about 50%) and calbindin. Interneurons ... calbindin D-28k or parvalbumin in monkey prefrontal cortex: distribution and morphology". J. Comp. Neurol. 341 (1): 95-116. doi ... the role of the calcium-binding proteins calbindin D-28k, calretinin and parvalbumin, in cerebellar physiology. Studies with ... 3.0.CO;2-7. PMID 8821450. Hashimoto T, Volk DW, Eggan SM, Mirnics K, Pierri JN, Sun Z, Sampson AR, Lewis DA (2003). "Gene ...
A specific, recognizable feature of Purkinje neurons is the expression of calbindin. Calbindin staining of rat brain after ... Whitney ER, Kemper TL, Rosene DL, Bauman ML, Blatt GJ (2008). "Calbindin-D28k is a more reliable marker of human Purkinje cells ... Retrieved 2 February 2015. Horvath S, Mah V, Lu AT, Woo JS, Choi OW, Jasinska AJ, Riancho JA, Tung S, Coles NS, Braun J, ... 44 (2-3): 103-116. doi:10.1016/j.brainresrev.2003.10.004. PMID 15003388. Felizola SJ, Nakamura Y, Ono Y, Kitamura K, Kikuchi K ...
"Calbindin 1, fibroblast growth factor 20, and alpha-synuclein in sporadic Parkinson's disease". Hum. Genet. 124 (1): 89-94. doi ... 2,4-dienoyl-CoA reductase 1 is a protein that in humans is encoded by the DECR1 gene. This gene encodes an accessory enzyme ... Alphey MS, Yu W, Byres E, Li D, Hunter WN (2005). "Structure and reactivity of human mitochondrial 2,4-dienoyl-CoA reductase: ... Yu W, Chu X, Chen G, Li D (2005). "Studies of human mitochondrial 2,4-dienoyl-CoA reductase". Arch. Biochem. Biophys. 434 (1): ...
However, calbindin and calretinin also show low levels of staining in the ventral nuclear group and in the medial and lateral ... A given region usually stains for two of the three proteins-parvalbumin, calbindin, and calretinin The neurons comprising the ... Moderate amounts of parvalbumin staining are also present in regions of the medio-dorsal nucleus (MD). By contrast, calbindin ... The primate thalamus consists of a matrix of calbindin immunoreactive cells and a superimposed core of parvalbumin ...
Compartmentalization of calcium binding proteins such as calretinin and calbindin-28 kDa has been noted within cells, ... calretinin and calbindin-28 kDa, in ganglion and amacrine cells of the rat retina". Molecular Vision. 14: 1600-1613. PMC ... Madame Curie Bioscience Database [Internet]. Retrieved 2 May 2016. Yáñez M, Gil-Longo J, Campos-Toimil M (2012). "Calcium ... doi:10.1007/978-94-007-2888-2_19. PMID 22453954. Siegel, George (Ed.). Basic neurochemistry: molecular, cellular and medical ...
... and calbindin. A small number of HSD2 neurons (less than 2%) may express the neuropeptide galanin. Their lack of expression of ... 497 (2): 223-50. doi:10.1002/cne.20993. PMID 16705681. Shin, JW; Geerling, JC; Loewy, AD (Dec 10, 2008). "Inputs to the ... 26 (2): 411-7. doi:10.1523/JNEUROSCI.3115-05.2006. PMID 16407537. Geerling, JC; Loewy, AD (Oct 18, 2006). "Aldosterone- ... 93 (2): 177-209. doi:10.1113/expphysiol.2007.039891. PMID 17981930. Geerling, JC; Loewy, AD (Mar 2007). "Sodium depletion ...
A specific, recognizable feature of Purkinje neurons is the expression of calbindin.[20] Calbindin staining of rat brain after ... Whitney ER, Kemper TL, Rosene DL, Bauman ML, Blatt GJ (February 2008). "Calbindin-D28k is a more reliable marker of human ... 174 (2): 329-45. doi:10.1002/cne.901740209. PMID 864041.. *^ a b Romer AS, Parsons TS (1977). The Vertebrate Body. Philadelphia ... 70 (2): 247-50. PMID 3299184.. *^ a b Hatten ME, Heintz N (1995). "Mechanisms of neural patterning and specification in the ...
... -D9k may also stimulate the basolateral calcium-pumping ATPases. Expression of calbindin-D9k, like that of calbindin- ... calbindin-D9k has two EF-hands, and calbindin-D28k has six. Calretinin is a 29kDa protein with 58% homology to calbindin-D28k ... Calbindin-D9k is present in mammalian intestinal epithelial cells (enterocytes). Calbindin-D9k can also be found in the kidney ... In the brain, its synthesis is independent of vitamin-D. There is no homology between calbindin-D28k and calbindin-D9k, apart ...
Calbindin is expressed by cerebellar Purkinje cells and granule cells of hippocampus. The reorganization and migration of ... calbindin-stained Purkinje neurons in rat cerebellum after peripheral nerve injury suggests that calbindin may be a marker for ... Microtubule-associated protein 2 (MAP-2) is a cytoskeletal protein. Its expression is weak in neuronal precursors but it ... 2 (1): 67-77. doi:10.1007/s12015-006-0011-1. Malatesta, P.; Appolloni, I.; Calzolari, F. (2008). "Radial glia and neural stem ...
... calbindin -calcitonin - calcitonin gene-related peptide - calcitonin gene-related peptide receptor - calcitonin receptor - ... Articles related to biochemistry include: Contents: Top 0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 2-amino-5- ... Bcl-2 - bcr-abl fusion protein - benzene - benzene ring - beta-2 microglobulin - beta adrenergic receptor - beta sheet - beta-1 ... leucine-2-alanine enkephalin - leukotriene B4 receptor - LH - LH receptor - LHRH receptor - life - life form - Ligand - light ...
Lutz W, Frank EM, Craig TA, Thompson R, Venters RA, Kojetin D, Cavanagh J, Kumar R (2003). "Calbindin D28K interacts with Ran- ... 2: 28. doi:10.1186/1471-2407-2-28. PMC 137593 . PMID 12421467. Ideguchi H, Ueda A, Tanaka M, Yang J, Tsuji T, Ohno S, Hagiwara ... 272 (1-2): 25-33. doi:10.1016/S0378-1119(01)00553-4. PMID 11470507. Caballero OL, Resto V, Patturajan M, Meerzaman D, Guo MZ, ... 2: 28. doi:10.1186/1471-2407-2-28. PMC 137593 . PMID 12421467. Hafizi S, Alindri F, Karlsson R, Dahlbäck B (2003). "Interaction ...
... calbindin and the calcium-pumping ATPase, PMCA1. The TRPV6 calcium transporter also found in the human placenta, pancreas and ... 280 (2): G285-90. PMID 11208552. Van Cromphaut SJ, Dewerchin M, Hoenderop JG, Stockmans I, Van Herck E, Kato S, Bindels RJ, ... Vitamin D treatment of human colon cancer cells, Caco-2, increased expression of TRPV6 transcripts, and also stimulated the ... mRNA expression in Caco-2 cells". Eur J Nutr. 45 (4): 196-204. doi:10.1007/s00394-005-0586-3. PMID 16362534. Balesaria S, ...
Woo TU, Shrestha K, Lamb D, Minns MM, Benes FM (April 2008). "N-Methyl-D-Aspartate Receptor and Calbindin-Containing Neurons in ... calbindin, targeting the dendrites of pyramidal neurons, and the expression of the mRNA for the GluR5 kainate receptor in GABA ... 13 (2): 623-31. PMID 8093901. Manev H, Favaron M, Guidotti A, Costa E (July 1989). "Delayed increase of Ca2+ influx elicited by ... J. 173 (2): 278-86. doi:10.1016/j.tvjl.2005.11.007. PMID 16376594. Kinnamon SC, Vandenbeuch A (July 2009). "Receptors and ...
Calbindin is a protein involved in calcium ion transport within cells, and excess calcium in cells is toxic. The calbindin ... Secondly, dopaminergic neurons in the pars compacta contain less calbindin than other dopaminergic neurons. ... "Midbrain Dopaminergic Neurons in the Mouse that Contain Calbindin-D28kExhibit Reduced Vulnerability to MPTP-induced ... 3.0.CO;2-E. PMID 10649295. Lima, Marcelo M.S.; Andersen, Monica L.; Reksidler, Angela B.; Vital, Maria A.B.F.; Tufik, Sergio ( ...
These cells, which show intense immunoreactivity for calbindin and calretinin, are characterized by their large size and axonal ... 3.0.CO;2-X. PMID 9699912. Neuronal Migration and Formation of Cellular Patterns During Brain Development - from the site of the ...
... s express somatostatin and sometimes calbindin, but not parvalbumin or vasoactive intestinal peptide. ... 15 (2): e2001392. doi:10.1371/journal.pbio.2001392. ISSN 1545-7885. PMC 5300109 . PMID 28182735. Silberberg G, Markram H (March ...
The territory is stained for calbindin. The mediator of the pallido-thalamic connection is the inhibitor GABA. The ... 4 (2): 539-560. PMID 6199485. Orioli, P.J.; Strick, P.L. (1989). "Cerebellar connectionswith the motor cortex and the arcuate ... Retinal axons from the controlateral retina end in stratae 1,4 and 6. Those from the ipsilateral retina end in 2,3 and 5. The ... Friedman, Jones E.G. (1986). "Thalamic input to area 3a and 2 in monkeys". J. Neurophysiol. 45 (59): 85. Kaas J.H., Nelson R.J ...
However, some reports have indicated that Renshaw cells synthesize calcium-binding proteins calbindin-D28k and parvalbumin. ... 197 (2): 83-97. doi:10.1111/j.1748-1716.2009.02020.x. PMID 19673737. Hultborn H (2006). "Spinal reflexes, mechanisms and ... 342 (2): 221-31. doi:10.1002/cne.903420206. PMID 8201033. Goshgarian, HG (2003). Neuroanatomic Organization of the Spinal Gray ... V0 neurons are commissural neurons that extend their axons rostrally for 2-4 spinal cord regions in the embryonic spinal cord. ...
The active protein is not highly structured, more than 50% of it adopting a random coil conformation.[2] When calcium binds ... The cardiac form Calsequestrin-2 (CASQ2) is present in cardiac and slow skeletal muscle and the fast skeletal form ... Both forms of calsequestrin are phosphorylated by casein kinase 2, but the cardiac form is phosphorylated more rapidly and to a ... there is a structural change whereby the alpha-helical content of the protein increases from 3 to 11%.[2] ...
Calretinin, CR, Calb2, calbindin 2.. Introduction. Calretinin is an intracellular calcium-binding protein belonging to the ...
Calbindin 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene ... CALB2 (Calbindin 2) is a Protein Coding gene. Diseases associated with CALB2 include Adenomatoid Tumor and Adenofibroma. GO ... The human calbindin D28k (CALB1) and calretinin (CALB2) genes are located at 8q21.3----q22.1 and 16q22----q23, respectively, ... The GeneCards human gene database index: 2 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z ...
Calbindin-D9k may also stimulate the basolateral calcium-pumping ATPases. Expression of calbindin-D9k, like that of calbindin- ... calbindin-D9k has two EF-hands, and calbindin-D28k has six. Calretinin is a 29kDa protein with 58% homology to calbindin-D28k ... Calbindin-D9k is present in mammalian intestinal epithelial cells (enterocytes). Calbindin-D9k can also be found in the kidney ... In the brain, its synthesis is independent of vitamin-D. There is no homology between calbindin-D28k and calbindin-D9k, apart ...
Calbindin D-28k occurs in all major pathways of the limbic system with the exception of the fornix. Calbindin D-28k is, however ... Springer, Berlin) is very rich in calbindin D-28k. The distribution of calbindin D-28k-positive neurons is very similar to that ... Calbindin D-28k is primarily associated with long-axon neurons (Golgi type I cells) exemplified by thalamic projection neurons ... Calbindin D-28k and parvalbumin in the rat nervous system.. Celio MR1. ...
CRF, Corticotropin-releasing hormone; CALB-1, calbindin 1; SOD2, superoxide dismutase 2; BCL-2, B-cell CLL/lymphoma 2; DRD1, ... Figure 2.. Age-dependent biological changes in neurons and glia. Known age-related cellular phenotypes are highlighted for ... Dopamine receptor D1; SYN2, synapsin II; GFAP, glial fibrillary acidic protein; NF-KB, nuclear factor kappa B; CNP, 2,3- ...
The solution structures of mutant calbindin D9ks, as determined by NMR, show that the calcium-binding site can adopt different ... CALBINDIN D9K A 76 Bos taurus Mutation: A15D, P20G, P43M Gene Name(s): S100G Gene View CALB3 S100D ... THE SOLUTION STRUCTURES OF MUTANT CALBINDIN D9KS, AS DETERMINED BY NMR, SHOW THAT THE CALCIUM BINDING SITE CAN ADOPT DIFFERENT ...
1-calbindin D9k reveals details of the stepwise structural changes along the Apo(Ca2+)II1(Ca2+)I,II2 binding pathway. ... CALBINDIN D9K A 76 Bos taurus Mutation: P43G Details: BOVINE MINOR A FORM, CADMIUM-HALF-SATURATED, CADMIUM ION IS BOUND IN C- ... Solution structure of (CD2+)1-calbindin D9K reveals details of the stepwise structural changes along the apo (CA2+)II1 ( ...
1IG5: Structural basis for the negative allostery between Ca(2+)- and Mg(2+)-binding in the intracellular Ca(2+)-receptor calbindin D9k.
29 kDa calbindin , calbindin 2, (29kD, calretinin) , calbindin D29K , calretinin , CR , calbindin 2, 29kDa , calbindin 2, 29kDa ... Zebrafish Calbindin 2 (CALB2) interaction partners * Downregulation of CRT gene may cause omphalocele in the Cd chick model by ... Show all anti-Calbindin 2 (CALB2) Antibodies with Pubmed References. * Chicken Polyclonal Calretinin Primary Antibody for IP, ... Human Calbindin 2 (CALB2) interaction partners * the Calreticulin deficiency-mediated increase in cell death was not prevented ...
29 kDa calbindin , calbindin 2, (29kD, calretinin) , calbindin D29K , calretinin , CR , calbindin 2, 29kDa , calbindin 2, 29kDa ... Zebrafish Calbindin 2 (CALB2) Interaktionspartner * Downregulation of CRT (zeige CALR Antikörper) gene may cause omphalocele in ... Show all anti-Calbindin 2 (CALB2) Antikörper with Pubmed References. * Human Monoclonal Calretinin Primary Antibody für BI, WB ... Mouse (Murine) Calbindin 2 (CALB2) Interaktionspartner * Absence of calretinin in mouse primary mesothelial cells inversely ...
The expression of calbindin 1 and 2 proteins in human tissues and common tumours outside the central nervous system ... The expression of calbindin 1 and 2 proteins in human tissues and common tumours outside the central nervous system ...
Although Calbindin-D9k (CaBP-9k), a cytosolic calcium binding protein which has calcium binding sites, is expressed in various ... Reproduced with permission from Yun S-M, Choi KC, Kim IH, An BS, Lee GS, Hong EJ, Son JH, Oh GT, Jeung E-B 2004 Calbindin-D9k ... Yun SM, Choi KC, Kim IH, An BS, Lee GS, Hong EJ, Oh GT, Jeung EB: Dominant expression of porcine Calbindin-D9k in the uterus ... An BS, Choi KC, Kang SK, Lee GS, Hong EJ, Hwang WS, Jeung EB: Mouse calbindin-D(9k) gene expression in the uterus during late ...
Calbindin-D28k and calretinin were both cloned from turtle brain cDNA. For calbindin-D28k, 243 of the 263 amino acid residues ... A, Calbindin-D28k-calretinin. Superimposed images of polyclonal anti-calbindin-D28k FITC fluorescence and monoclonal anti- ... 8). Labeling of the stereocilia was more conspicuous than with calbindin-D28k, and, similar to calbindin-D28k, was marked ... The subcellular distribution of calbindin-D28k and calretinin within the hair cells was quite different. Calbindin-D28k ...
All of the doubly labeled (BrdU-FG) neurons also immunolabeled with the antibody to calbindin-D28k. Many doubly labeled (BrdU- ... and either calbindin-D28k or synaptophysin immunohistochemistry. A large proportion (up to 44%) of BrdU-labeled cells in the ... In the present study, 9 weeks after a series of intraperitoneal injections of 5-bromo-2-deoxyuridine (BrdU), animals received ...
Calbindin (CalB). Calbindin (CalB) neurons are also found throughout the core and shell subdivisions of the SCN. Silver and co- ... Like calbindin (CalB), CALR is a calcium binding protein. Apparently, there is a developmental reduction in Calbindin-D28k ... Silver, R, Romero, MT, Besmer, HR, Leak, R and Nunez, JM (1996). Calbindin-D28K cells in the hamster SCN express light-induced ... Hamada, T, LeSauter, J, Lokshin, M, Romero, MT, Yan, L, Venuti, JM and Silver, R (2003). Calbindin influences response to ...
Calbindin 2. A calbindin protein that is differentially expressed in distinct populations of NEURONS throughout the vertebrate ... Pleural catheter will be placed first, then pts will receive 2 doses of intrapleural vector followed by front line or second ...
Additionally, calcium pump activity and calbindin-D28k expression were dramatically increased after GHE treatment, implicating ... 0/calbindin; 2552-55-8/Ibotenic Acid; EC 3.4.22.-/Caspase 3; EC 3.6.1.8/Calcium-Transporting ATPases ... Proto-Oncogene Proteins c-bcl-2 / metabolism. Rats. Rats, Sprague-Dawley. Time Factors. bcl-2-Associated X Protein / metabolism ... 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein; ...
Hypercalciuria during space flight: the role of calbindin D28K. * Giovambattista Capasso, M.D.. This experiment investigates ... The importance of the membrane-transporter protein calbindin for calcium re-absorption is being investigated. In particular the ... 2012; 26(2): 30-37. Forsman AD, Nier HA. The effects of spaceflight on mucin production in the mouse uterus. Gravitational and ... CBTM-2 is also expected to contribute data to the current body of research on microgravity effects on organ systems through a ...
Calbindin 28K and calretinin are very similar calcium binding proteins which are both present in the central nervous system ( ... Calbindin 28K is also present in the endocrine system. We have examined the cellular distribution of calbindin in the ... Calbindin 28K and calretinin are very similar calcium binding proteins which are both present in the central nervous system ( ... Pochet R, Pipeleers DG and Malaisse WJ (1987). Calbindin D 27 kDa: preferential localization in non-B islet cells of the rat ...
Mutants displayed reduced Purkinje cell numbers by Calbindin staining. B. PC loss occurred by 2 months in mutants. No PC loss ... for B-C, controls/mutants: n=3 mice; hets, n=2 mice; ,500 cells/group) D., E. Mutant PCs displayed increased spine density. ( ... Quantification of cell numbers on right (n , 500cells; 2 mice per group) B., C. Whereas vehicle treated mutants displayed ... Scale bars in A.100μm; D. 20μm (left), 5μm (middle), 2μm (right). ...
1994) in which calbindin protein staining was used. The circles represent locations where LTP was elicited in the Nacb to Fo/Fi ... In these sections, the representative shell/core border based on differential calbindin-protein immunoreactivity (Jongen-Rêlo ... 2). The time courses of the LTP of the Fo/Fi to Nacb pathway and the LTD of the BLA to Nacb pathway for the six rats are shown ... Table 2. Firing probability of Nacb single units (n= 10) to either BLA or Fo/Fi stimulation or combinations of BLA and Fo/Fi ...
2005, 2: 345-350. 10.1038/nmeth756.PubMedView ArticleGoogle Scholar. *. Guo L, Lobenhofer EK, Wang C, Shippy R, Harris SC, ... 10.1016/S0169-328X(03)00288-2.PubMedView ArticleGoogle Scholar. *. Bailey DJ, Wade J: FOS and ZENK responses in 45-day-old ... 10.1016/0169-328X(94)90013-2.PubMedView ArticleGoogle Scholar. *. Velho TA, Pinaud R, Rodrigues PV, Mello CV: Co-induction of ... 10.1016/S0959-437X(99)00022-2.PubMedView ArticleGoogle Scholar. *. Griffiths-Jones S, Grocock RJ, van Dongen S, Bateman A, ...
This gene encodes calbindin D9K, a vitamin D-dependent calcium-binding protein. This cytosolic protein belongs to a family of ... Jeung EB, Krisinger J, Dann JL, Leung PC (Sep 1992). "Molecular cloning of the full-length cDNA encoding the human calbindin- ... 2004). "Control of differentiation-induced calbindin-D9k gene expression in Caco-2 cells by cdx-2 and HNF-1α". Am. J. Physiol. ... "Entrez Gene: S100G S100 calcium binding protein G". Balmain N (1991). "Calbindin-D9k. A vitamin-D-dependent, calcium-binding ...
Belongs to the calbindin family.. Contains 6 EF-hand domains.. * Target information above from: UniProt accession P22676. The ...
The calbindin marker for Purkinje neurons revealed a partial loss of these cells in the cerebellum and a profound swelling of ... calbindin-labeled Purkinje neurons were reduced in number and exhibited swollen dendrites in the mutant mice (Q). (R) The ... rabbit Ab anti-calbindin D-28 (1:500; Swant, Bellinzona, Switzerland), rabbit anti-cleaved caspase 3 (1:100; Cell Signaling ... 2 G), as reported in patients with type 2 GD (11, 12, 15). The cerebellum and nuclei of the pons and medulla also displayed a ...
  • We have examined the cellular distribution of calbindin in the pancreatic endocrine cells of chick, rat and human and found variable distribution among the different endocrine cell types. (springer.com)
  • As a general characterization it can be stated that calbindin antibodies mainly label cells with thin, unmyelinated axons projecting in a diffuse manner. (nih.gov)
  • syndecan-3 is concentrated in axons, whereas syndecan-2 is localized in synapses. (jneurosci.org)
  • b and c , Anti-calbindin IF ( b ) and Timm stain ( c ) shows that IPB axons in 8-10 week old adult EB3 −/− mice are much longer than in WT littermates (distance between arrowheads). (nih.gov)
  • a , Anti-calbindin IF shows long IPB axons in indicated EphB single and compound mutants at 8-10 weeks. (nih.gov)
  • PTH up-regulates 25-hydroxyvitamin D3 1-alpha-hydroxylase, the enzyme responsible for 1-alpha hydroxylation of 25-hydroxy vitamin D, converting vitamin D to its active form (1,25-dihydroxy vitamin D). This activated form of vitamin D increases the absorption of calcium (as Ca2+ ions) by the intestine via calbindin. (wikipedia.org)
  • In the present study, 9 weeks after a series of intraperitoneal injections of 5-bromo-2'-deoxyuridine (BrdU), animals received stereotaxic iontophoretic injections of Fluoro-Gold (FG) into field CA3. (nih.gov)
  • Monkeys (male Macaca fascicularis ) were given 5-bromo-2-deoxyuridine (BrdU) injections 2-3 weeks after the rhizotomy, and were perfused 4-6 weeks later. (jneurosci.org)
  • A significant number of BrdU/NeuN- and BrdU/calbindin-colabeled cells were observed in topographically reorganized cortex. (jneurosci.org)
  • Consistently the number of labeled cells in females is no longer higher than that observed in males by 2 weeks after the last BrdU injection. (jneurosci.org)
  • Grid cells have been described mainly but not exclusively [ 2 ] in the medial entorhinal cortex (MEC) and form a robust allocentric representation of space [ 1 ]. (royalsocietypublishing.org)
  • A consensus has emerged that, of the different layers of entorhinal cortex, layer 2 contains the largest fraction of and the clearest grid cells [ 2 ]. (royalsocietypublishing.org)
  • The inspiration for the hypothesis does not come from temporal dynamics (i.e. phase precession) or attractor network theory [ 21 ], but it comes from the modular anatomy of layer 2 of the entorhinal cortex [ 22 - 24 ]. (royalsocietypublishing.org)
  • In embryos from Carnegie stages (CS) 17 to 23, calbindin (CB) and CR stain opposite poles of the incipient cortex suggesting early regionalization: CB marks the neuroepithelium of the medial boundary of the cortex with the choroid plexus (cortical hem). (frontiersin.org)
  • 2) Studying expression of schizophrenia susceptibility genes in the early fetal human thalamus and cerebral cortex. (ncl.ac.uk)
  • The syndecan family of cell surface heparan sulfate proteoglycans interacts via their cytoplasmic C-terminal tail with the PDZ domain of CASK/LIN-2, a membrane-associated guanylate kinase homolog. (jneurosci.org)
  • CASK is a cytoplasmic-binding partner for syndecans, and its subcellular distribution changes strikingly during development, shifting from a primarily axonal distribution in the first 2 postnatal weeks to a somatodendritic distribution in adult brain. (jneurosci.org)
  • We also observed a subpopulation of PCs that were misplaced in the inner granule cell layer of Bax-KO mice on postnatal day 5 (P5) to P10 and that, by the end of the major period of cerebellar histogenesis (P14), lose expression of the PC marker calbindin. (jneurosci.org)
  • Upon binding to Ca2+, this motif may undergo conformational changes that enable Ca2+-regulated functions as seen in Ca2+ effectors such as calmodulin (CaM) and troponin C (TnC) and Ca2+ buffers such as calreticulin and calbindin D9k. (wikipedia.org)
  • e.g., calbindin in CA3 of naked mole-rats distributes similar to the pattern seen in early primate development, and calbindin staining extends into the stratum lacunosum-moleculare of Cape mole-rats. (frontiersin.org)
  • Bernier PJ, Parent A (1998b) The anti-apoptosis bcl-2 proto-oncogene is preferentially expressed in limbic structures of the primate brain. (springer.com)
  • Calbindin-D9k mediates the transport of calcium across the enterocytes from the apical side, where entry is regulated by the calcium channel TRPV6, to the basolateral side, where calcium pumps such as PMCA1 utilize intracellular adenosine triphosphate to pump calcium into the blood. (wikipedia.org)
  • Shp2, an Src-homology 2 domain (SH2)-containing tyrosine phosphatase, is a widely expressed intracellular enzyme that regulates signaling events downstream of several growth factor/cytokine receptors in various cell types ( 8 , 18 ). (asm.org)
  • The retrograde suppression of the synaptic transmission by the endocannabinoid sn-2-arachidonoylglycerol (2-AG) is mediated by the cannabinoid CB1 receptors and requires the elevation of intracellular Ca(2+) and the activation of specific 2-AG synthesizing (i.e. (sigmaaldrich.com)
  • 2016. Mode of action of DNA-competitive small molecule inhibitors of tyrosyl DNA phosphodiesterase 2 . (cardiff.ac.uk)
  • Recientes caracterizaciones en los cambios en la expresión génica dependiente de la edad han mostrado que el envejecimiento del cerebro humano requiere de un conjunto específico de vías biológicas en un continuo a través del curso de toda la vida, y que los mismos genes que están asociados con el envejecimiento normal se implican frecuentemente y de igual forma en la depresión como en otros trastornos cerebrates. (nih.gov)
  • Additional genes that have been shown, either by in vitro or in vivo studies, to be regulated by epigenetic mechanisms include leptin, SOCS3, glucose transporter (GLUT)-4, POMC, 11-β-hydroxysteroid dehydrogenase type 2 and corticotrophin releasing hormone. (wikipedia.org)