AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPsychiatry and PsychologyPhenomena and ProcessesInformation Science
Caenorhabditis elegans ProteinsCaenorhabditis elegansHelminth ProteinsGenes, HelminthMolecular Sequence DataCaenorhabditisAmino Acid SequenceSequence Homology, Amino AcidVulvaDNA, HelminthMutationAnimals, Genetically ModifiedRNA, HelminthGenome, HelminthDisorders of Sex DevelopmentBase SequenceCloning, MolecularLongevityRNA InterferenceGerm CellsPhenotypeNematodaEmbryo, NonmammalianLarvaGene Expression Regulation, DevelopmentalAldicarbGonadsGreen Fluorescent ProteinsPharynxSequence AlignmentTranscription FactorsSignal TransductionOvipositionConserved SequenceLocomotionGenes, LethalAllelesMembrane ProteinsChromosome MappingAntinematodal AgentsGATA Transcription FactorsHermaphroditic OrganismsMeiosisModels, BiologicalLuminescent ProteinsGene Expression RegulationMoltingRecombinant Fusion ProteinsDNA, ComplementarySex Determination ProcessesLevamisoleMorphogenesisPhylogenyNeuronsPharyngeal MusclesNervous SystemProtein Structure, TertiaryEvolution, MolecularGenomeSuppression, GeneticTransgenesModels, Genetic
Caenorhabditis elegans Proteins
Proteins from the nematode species CAENORHABDITIS ELEGANS. The proteins from this species are the subject of scientific interest in the area of multicellular organism MORPHOGENESIS.
Caenorhabditis elegans
A species of nematode that is widely used in biological, biochemical, and genetic studies.
Helminth Proteins
Proteins found in any species of helminth.
Genes, Helminth
The functional hereditary units of HELMINTHS.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Caenorhabditis
A genus of small free-living nematodes. Two species, CAENORHABDITIS ELEGANS and C. briggsae are much used in studies of genetics, development, aging, muscle chemistry, and neuroanatomy.
Amino Acid Sequence
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Sequence Homology, Amino Acid
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
Vulva
The external genitalia of the female. It includes the CLITORIS, the labia, the vestibule, and its glands.
DNA, Helminth
Deoxyribonucleic acid that makes up the genetic material of helminths.
Mutation
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Animals, Genetically Modified
ANIMALS whose GENOME has been altered by GENETIC ENGINEERING, or their offspring.
RNA, Helminth
Ribonucleic acid in helminths having regulatory and catalytic roles as well as involvement in protein synthesis.
Genome, Helminth
The genetic complement of a helminth (HELMINTHS) as represented in its DNA.
Disorders of Sex Development
In gonochoristic organisms, congenital conditions in which development of chromosomal, gonadal, or anatomical sex is atypical. Effects from exposure to abnormal levels of GONADAL HORMONES in the maternal environment, or disruption of the function of those hormones by ENDOCRINE DISRUPTORS are included.
Base Sequence
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Cloning, Molecular
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Longevity
The normal length of time of an organism's life.
RNA Interference
A gene silencing phenomenon whereby specific dsRNAs (RNA, DOUBLE-STRANDED) trigger the degradation of homologous mRNA (RNA, MESSENGER). The specific dsRNAs are processed into SMALL INTERFERING RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA-INDUCED SILENCING COMPLEX. DNA METHYLATION may also be triggered during this process.
Germ Cells
The reproductive cells in multicellular organisms at various stages during GAMETOGENESIS.
Phenotype
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
Nematoda
A class of unsegmented helminths with fundamental bilateral symmetry and secondary triradiate symmetry of the oral and esophageal structures. Many species are parasites.
Embryo, Nonmammalian
The developmental entity of a fertilized egg (ZYGOTE) in animal species other than MAMMALS. For chickens, use CHICK EMBRYO.
Larva
Wormlike or grublike stage, following the egg in the life cycle of insects, worms, and other metamorphosing animals.
Gene Expression Regulation, Developmental
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action during the developmental stages of an organism.
Aldicarb
Carbamate derivative used as an insecticide, acaricide, and nematocide.
Gonads
The gamete-producing glands, OVARY or TESTIS.
Green Fluorescent Proteins
Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.
Pharynx
A funnel-shaped fibromuscular tube that conducts food to the ESOPHAGUS, and air to the LARYNX and LUNGS. It is located posterior to the NASAL CAVITY; ORAL CAVITY; and LARYNX, and extends from the SKULL BASE to the inferior border of the CRICOID CARTILAGE anteriorly and to the inferior border of the C6 vertebra posteriorly. It is divided into the NASOPHARYNX; OROPHARYNX; and HYPOPHARYNX (laryngopharynx).
Sequence Alignment
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Transcription Factors
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
Signal Transduction
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Oviposition
The process of laying or shedding fully developed eggs (OVA) from the female body. The term is usually used for certain INSECTS or FISHES with an organ called ovipositor where eggs are stored or deposited before expulsion from the body.
Conserved Sequence
A sequence of amino acids in a polypeptide or of nucleotides in DNA or RNA that is similar across multiple species. A known set of conserved sequences is represented by a CONSENSUS SEQUENCE. AMINO ACID MOTIFS are often composed of conserved sequences.
Locomotion
Movement or the ability to move from one place or another. It can refer to humans, vertebrate or invertebrate animals, and microorganisms.
Genes, Lethal
Genes whose loss of function or gain of function MUTATION leads to the death of the carrier prior to maturity. They may be essential genes (GENES, ESSENTIAL) required for viability, or genes which cause a block of function of an essential gene at a time when the essential gene function is required for viability.
Alleles
Variant forms of the same gene, occupying the same locus on homologous CHROMOSOMES, and governing the variants in production of the same gene product.
Membrane Proteins
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Chromosome Mapping
Any method used for determining the location of and relative distances between genes on a chromosome.
Antinematodal Agents
Substances used in the treatment or control of nematode infestations. They are used also in veterinary practice.
GATA Transcription Factors
A family of transcription factors that contain two ZINC FINGER MOTIFS and bind to the DNA sequence (A/T)GATA(A/G).
Hermaphroditic Organisms
Animals and plants which have, as their normal mode of reproduction, both male and female sex organs in the same individual.
Meiosis
A type of CELL NUCLEUS division, occurring during maturation of the GERM CELLS. Two successive cell nucleus divisions following a single chromosome duplication (S PHASE) result in daughter cells with half the number of CHROMOSOMES as the parent cells.
Models, Biological
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
Luminescent Proteins
Proteins which are involved in the phenomenon of light emission in living systems. Included are the "enzymatic" and "non-enzymatic" types of system with or without the presence of oxygen or co-factors.
Gene Expression Regulation
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
Molting
Periodic casting off FEATHERS; HAIR; or cuticle. Molting is a process of sloughing or desquamation, especially the shedding of an outer covering and the development of a new one. This phenomenon permits growth in ARTHROPODS, skin renewal in AMPHIBIANS and REPTILES, and the shedding of winter coats in BIRDS and MAMMALS.
Recombinant Fusion Proteins
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
DNA, Complementary
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
Sex Determination Processes
The mechanisms by which the SEX of an individual's GONADS are fixed.
Levamisole
An antihelminthic drug that has been tried experimentally in rheumatic disorders where it apparently restores the immune response by increasing macrophage chemotaxis and T-lymphocyte function. Paradoxically, this immune enhancement appears to be beneficial in rheumatoid arthritis where dermatitis, leukopenia, and thrombocytopenia, and nausea and vomiting have been reported as side effects. (From Smith and Reynard, Textbook of Pharmacology, 1991, p435-6)
Morphogenesis
The development of anatomical structures to create the form of a single- or multi-cell organism. Morphogenesis provides form changes of a part, parts, or the whole organism.
Phylogeny
The relationships of groups of organisms as reflected by their genetic makeup.
Neurons
The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.
Pharyngeal Muscles
The muscles of the PHARYNX are voluntary muscles arranged in two layers. The external circular layer consists of three constrictors (superior, middle, and inferior). The internal longitudinal layer consists of the palatopharyngeus, the salpingopharyngeus, and the stylopharyngeus. During swallowing, the outer layer constricts the pharyngeal wall and the inner layer elevates pharynx and LARYNX.
Nervous System
The entire nerve apparatus, composed of a central part, the brain and spinal cord, and a peripheral part, the cranial and spinal nerves, autonomic ganglia, and plexuses. (Stedman, 26th ed)
Protein Structure, Tertiary
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Evolution, Molecular
The process of cumulative change at the level of DNA; RNA; and PROTEINS, over successive generations.
Genome
The genetic complement of an organism, including all of its GENES, as represented in its DNA, or in some cases, its RNA.
Suppression, Genetic
Mutation process that restores the wild-type PHENOTYPE in an organism possessing a mutationally altered GENOTYPE. The second "suppressor" mutation may be on a different gene, on the same gene but located at a distance from the site of the primary mutation, or in extrachromosomal genes (EXTRACHROMOSOMAL INHERITANCE).
Transgenes
Genes that are introduced into an organism using GENE TRANSFER TECHNIQUES.
Models, Genetic
Theoretical representations that simulate the behavior or activity of genetic processes or phenomena. They include the use of mathematical equations, computers, and other electronic equipment.

Regulation of body length and male tail ray pattern formation of Caenorhabditis elegans by a member of TGF-beta family. (1/5831)

We have identified a new member of the TGF-beta superfamily, CET-1, from Caenorhabditis elegans, which is expressed in the ventral nerve cord and other neurons. cet-1 null mutants have shortened bodies and male tail abnormal phenotype resembling sma mutants, suggesting cet-1, sma-2, sma-3 and sma-4 share a common pathway. Overexpression experiments demonstrated that cet-1 function requires wild-type sma genes. Interestingly, CET-1 appears to affect body length in a dose-dependent manner. Heterozygotes for cet-1 displayed body lengths ranging between null mutant and wild type, and overexpression of CET-1 in wild-type worms elongated body length close to lon mutants. In male sensory ray patterning, lack of cet-1 function results in ray fusions. Epistasis analysis revealed that mab-21 lies downstream and is negatively regulated by the cet-1/sma pathway in the male tail. Our results show that cet-1 controls diverse biological processes during C. elegans development probably through different target genes.  (+info)

Alzheimer's disease: clues from flies and worms. (2/5831)

Presenilin mutations give rise to familial Alzheimer's disease and result in elevated production of amyloid beta peptide. Recent evidence that presenilins act in developmental signalling pathways may be the key to understanding how senile plaques, neurofibrillary tangles and apoptosis are all biochemically linked.  (+info)

The Caenorhabditis elegans sex determination gene mog-1 encodes a member of the DEAH-Box protein family. (3/5831)

In the Caenorhabditis elegans hermaphrodite germ line, the sex-determining gene fem-3 is repressed posttranscriptionally to arrest spermatogenesis and permit oogenesis. This repression requires a cis-acting regulatory element in the fem-3 3' untranslated region; the FBF protein, which binds to this element; and at least six mog genes. In this paper, we report the molecular characterization of mog-1 as well as additional phenotypic characterization of this gene. The mog-1 gene encodes a member of the DEAH-box family. Three mog-1 alleles possess premature stop codons and are likely to be null alleles, and one is a missense mutation and is likely to retain residual activity. mog-1 mRNA is expressed in both germ line and somatic tissues and appears to be ubiquitous. The MOG-1 DEAH-box protein is most closely related to proteins essential for splicing in the yeast Saccharomyces cerevisiae, but splicing appears to occur normally in a mog-1-null mutant. In addition to its involvement in the sperm-oocyte switch and control of fem-3, zygotic mog-1 is required for robust germ line proliferation and for normal growth during development. We suggest that mog-1 plays a broader role in RNA regulation than previously considered.  (+info)

Characterization of a Caenorhabditis elegans recA-like gene Ce-rdh-1 involved in meiotic recombination. (4/5831)

A recA-like gene was identified in the Caenorhabditis elegans genome project database. The putative product of the gene, termed Ce-rdh-1 (C. elegans RAD51 and DMC1/LIM15 homolog 1), consists of 357 amino acid residues. The predicted amino acid sequence of Ce-rdh-1 showed 46-60% identity to both RAD51 type and DMC1/LIM15 type genes in several eukaryote species. The results of RNAi (RNA-mediated interference) indicated that repression of Ce-rdh-1 blocked chromosome condensation of six bivalents and dissociation of chiasmata in oocytes of F1 progeny. Oogenesis did not proceed to the diakinesis stage. Accordingly, all the eggs produced (F2) died in early stages. These results suggest that Ce-rdh-1 participates in meiotic recombination.  (+info)

The Caenorhabditis elegans gene ham-2 links Hox patterning to migration of the HSN motor neuron. (5/5831)

The Caenorhabditis elegans HSN motor neurons permit genetic analysis of neuronal development at single-cell resolution. The egl-5 Hox gene, which patterns the posterior of the embryo, is required for both early (embryonic) and late (larval) development of the HSN. Here we show that ham-2 encodes a zinc finger protein that acts downstream of egl-5 to direct HSN cell migration, an early differentiation event. We also demonstrate that the EGL-43 zinc finger protein, also required for HSN migration, is expressed in the HSN specifically during its migration. In an egl-5 mutant background, the HSN still expresses EGL-43, but expression is no longer down-regulated at the end of the cell's migration. Finally, we find a new role in early HSN differentiation for UNC-86, a POU homeodomain transcription factor shown previously to act downstream of egl-5 in the regulation of late HSN differentiation. In an unc-86; ham-2 double mutant the HSNs are defective in EGL-43 down-regulation, an egl-5-like phenotype that is absent in either single mutant. Thus, in the HSN, a Hox gene, egl-5, regulates cell fate by activating the transcription of genes encoding the transcription factors HAM-2 and UNC-86 that in turn individually control some differentiation events and combinatorially affect others.  (+info)

Patterning of Caenorhabditis elegans posterior structures by the Abdominal-B homolog, egl-5. (6/5831)

The Caenorhabditis elegans body axis, like that of other animals, is patterned by the action of Hox genes. In order to examine the function of one C. elegans Hox gene in depth, we determined the postembryonic expression pattern of egl-5, the C. elegans member of the Abdominal-B Hox gene paralog group, by means of whole-mount staining with a polyclonal antibody. A major site of egl-5 expression and function is in the epithelium joining the posterior digestive tract with the external epidermis. Patterning this region and its derived structures is a conserved function of Abd-B paralog group genes in other animals. Cells that initiate egl-5 expression during embryogenesis are clustered around the presumptive anus. Expression is initiated postembryonically in four additional mesodermal and ectodermal cell lineages or tissues. Once initiated in a lineage, egl-5 expression continues throughout development, suggesting that the action of egl-5 can be regarded as defining a positional cell identity. A variety of cross-regulatory interactions between egl-5 and the next more anterior Hox gene, mab-5, help define the expression domains of their respective gene products. In its expression in a localized body region, function as a marker of positional cell identity, and interactions with another Hox gene, egl-5 resembles Hox genes of other animals. This suggests that C. elegans, in spite of its small cell number and reproducible cell lineages, may not differ greatly from other animals in the way it employs Hox genes for regional specification during development.  (+info)

Merbarone, a catalytic inhibitor of DNA topoisomerase II, induces apoptosis in CEM cells through activation of ICE/CED-3-like protease. (7/5831)

Merbarone (5-[N-phenyl carboxamido]-2-thiobarbituric acid) is an anticancer drug that inhibits the catalytic activity of DNA topoisomerase II (topo II) without damaging DNA or stabilizing DNA-topo II cleavable complexes. Although the cytotoxicity of the complex-stabilizing DNA-topo II inhibitors such as VP-16 (etoposide) has been partially elucidated, the cytotoxicity of merbarone is poorly understood. Here, we report that merbarone induces programmed cell death or apoptosis in human leukemic CEM cells, characterized by internucleosomal DNA cleavage and nuclear condensation. Treatment of CEM cells with apoptosis-inducing concentrations of merbarone caused activation of c-Jun NH2-terminal kinase/stress-activated protein kinase, c-jun gene induction, activation of caspase-3/CPP32-like protease but not caspase-1, and the proteolytic cleavage of poly(ADP-ribose) polymerase. Treatment of CEM cells with a potent inhibitor of caspases, Z-Asp-2. 6-dichlorobenzoyloxymethyl-ketone, inhibited merbarone-induced caspase-3/CPP32-like activity and apoptosis in a dose-dependent manner. These results indicate that the catalytic inhibition of topo II by merbarone leads to apoptotic cell death through a caspase-3-like protease-dependent mechanism. These results further suggest that c-Jun and c-Jun NH2-terminal kinase/stress-activated protein kinase signaling may be involved in the cytotoxicity of merbarone.  (+info)

Interaction of 5-lipoxygenase with cellular proteins. (8/5831)

5-Lipoxygenase (5LO) plays a pivotal role in cellular leukotriene synthesis. To identify proteins interacting with human 5LO, we used a two-hybrid approach to screen a human lung cDNA library. From a total of 1.5 x 10(7) yeast transformants, nine independent clones representing three different proteins were isolated and found to specifically interact with 5LO. Four 1.7- to 1.8-kb clones represented a 16-kDa protein named coactosin-like protein for its significant homology with coactosin, a protein found to be associated with actin in Dictyostelium discoideum. Coactosin-like protein thus may provide a link between 5LO and the cytoskeleton. Two other yeast clones of 1.5 kb encoded transforming growth factor (TGF) type beta receptor-I-associated protein 1 partial cDNA. TGF type beta receptor-I-associated protein 1 recently has been reported to associate with the activated form of the TGF beta receptor I and may be involved in the TGF beta-induced up-regulation of 5LO expression and activity observed in HL-60 and Mono Mac 6 cells. Finally, three identical 2.1-kb clones contained the partial cDNA of a human protein with high homology to a hypothetical helicase K12H4. 8 from Caenorhabditis elegans and consequently was named DeltaK12H4. 8 homologue. Analysis of the predicted amino acid sequence revealed the presence of a RNase III motif and a double-stranded RNA binding domain, indicative of a protein of nuclear origin. The identification of these 5LO-interacting proteins provides additional approaches to studies of the cellular functions of 5LO.  (+info)

A Conserved p38 MAP Kinase Pathway in Caenorhabditis elegans Innate Immunity | ScienceA Conserved p38 MAP Kinase Pathway in Caenorhabditis elegans Innate Immunity | Science
esp-8 was mapped to a 400-kb region of chromosome II and rescued with a 13-kb fragment containing the F59A6.1 coding sequence and 3.8 kb of upstream promoter sequence. F59A6.1 encodes the C. elegans ortholog of the mammalian MAPKKK ASK1 that has recently been found to correspond to the nsy-1 locus (9). The esp-8/nsy-1(ag3) mutation is a C-to-T change that converts the codon for Gln1013 to a premature stop codon just after the kinase domain. Another putative null allele,nsy-1(ky397) (9, 10), also showed sensitivity to PA14 comparable to esp-8/nsy-1(ag3) (Fig. 3A). Genetic and biochemical data suggest that NSY-1 is a direct activator of SEK-1 in the signaling pathway mediating asymmetric neuronal cell fate in AWC sensory neurons (8).. Although the Nsy phenotype corresponds to nsy-1 andsek-1 function in the AWC neurons, nsy-1 andsek-1 are expressed in a number of tissues types, including the intestine (8, 9). Whereas the signal transduction pathways that are involved in the Nsy and Esp phenotypes ...
https://science.sciencemag.org/content/297/5581/623?ijkey=09fb1edcd09c7c846ff5405a9550db845ceab683&keytype2=tf_ipsecsha
Search Articles | University of Toronto Libraries
Forkhead box O ( FoxO) transcription factors FoxO1, FoxO3a, FoxO4 and FoxO6, the mammalian orthologs of Caenorhabditis elegans DAF-16, are emerging as an important family of proteins that modulate the expression of genes ...
https://query.library.utoronto.ca/index.php/search/q?kw=SubjectTerms:Forkhead%20Transcription%20Factors%20-%20metabolism
TumorPortal
This gene is located on the long arm of chromosome 4 in a region that is associated with susceptibility to celiac disease. The encoded protein is similar to a Chinese hamster protein that is associated with spermatocyte and adipocyte differentiation. The C-terminus of the protein is also similar to a Caenorhabditis elegans protein that plays a role in lipid storage. In mammals, this protein is thought to function in the regulation of epithelial growth and differentiation, and in tumor development ...
http://www.tumorportal.org/view?geneSymbol=KIAA1109
The cisd gene family regulates physiological germline apoptosis through ced-13 and the canonical cell death pathway in...The cisd gene family regulates physiological germline apoptosis through ced-13 and the canonical cell death pathway in...
The cisd gene family regulates physiological germline apoptosis through ced-13 and the canonical cell death pathway in Caenorhabditis ...
https://bondlsc.missouri.edu/publication/the-cisd-gene-family-regulates-physiological-germline-apoptosis-through-ced-13-and-the-canonical-cell-death-pathway-in-caenorhabditis-elegans/
The dve-1 gene and its possible relation to ageing in roundwormsThe dve-1 gene and its possible relation to ageing in roundworms
Several of the RNAi candidates (dve-1; lin-40; nhr-49; ceh-20; lin-11; and nhr-77) appeared to non-discriminately shorten lifespan in all strains tested, suggesting that the corresponding transcription factors are broadly required for survival. Interestingly, four RNAi clones (ZC123.3; nhr-119; ceh-37; and aha-1) affected wild-type and isp-1;ctb-1 mutant worms lifespan to the same extent but exerted only a moderate or no effect on daf-16 and age-1 mutant longevity ...
http://genomics.senescence.info/genes/details.php?id=915
Positioning edgetic residues in CED-9 structures. (a) P | Open-iPositioning edgetic residues in CED-9 structures. (a) P | Open-i
Positioning edgetic residues in CED-9 structures. (a) Positions of edgetic residues in the CED-9 sequence. The portion of CED-9 present in the crystal (PDB ID c
https://openi.nlm.nih.gov/detailedresult.php?img=PMC2865203_nihms186337f5&req=4
Implications of sequencing the nematode Caenorhabditis elegans genome for plant nematologyImplications of sequencing the nematode Caenorhabditis elegans genome for plant nematology
1. Baldwin, J.G., Nadler, S.A., and Wall, D.H. 1997. Nematodes: Pervading the Earth and Linking all Life. Pp. 176-191. In: Raven, P.H. (ed.). National Academy Press, Washington, D.C. 625 pp.. 2. Bargmann, C. I. 1998. Neurobiology of the Caenorhabditis elegans genome. Science 282:2028-2033.. 3. Bargmann, C. I. And Mori, I. 1997. Chemotaxis and Thermotaxis. Pp. 717-737. In: Riddle, D.L., Blumenthal, T., Meyer, B.J. and Priess, J.R. (eds). C. elegans II. Cold Spring Harbor Laboratory Press, Plainview, NY 1222 pp.. 4. Bird, D.M. and Opperman, C. H. 1998. Caenorhabditis elegans. J. Nematol. 30:299-308.. 5. Bird, D.M., Opperman, C.H., Jones S.J.M., and Baillie, D.L. 1999. The Caenorhabditis elegans gemome: a guide in the post genomics age. Annu. Rev. Phytopathol. 37:247-265.. 6. Blaxter, M. 1998. Caenorhabditis elegans is a nematode. Science 282:2041-2046.. 7. Blaxter, M. and Bird, D. 1997. Parasitic nematodes. Pp. 851-878. In: Riddle, D.L., Blumenthal, T., Meyer, B.J. and Priess, J.R. (eds). C. ...
https://www.apsnet.org/publications/apsnetfeatures/Pages/Celegans.aspx
Decreased expression of myogenic transcription factors and myosin heavy chains in Caenorhabditis elegans muscles developed...Decreased expression of myogenic transcription factors and myosin heavy chains in Caenorhabditis elegans muscles developed...
The molecular mechanisms underlying muscle atrophy during spaceflight are not well understood. We have analyzed the effects of a 10-day spaceflight on Caenorhabditis elegans muscle development. DNA microarray, real-time quantitative PCR, and quantitative western blot analyses revealed that the amount of MHC in both body-wall and pharyngeal muscle decrease in response to spaceflight. Decreased transcription of the body-wall myogenic transcription factor HLH-1 (CeMyoD) and of the three pharyngeal myogenic transcription factors, PEB-1, CEH-22 and PHA-4 were also observed. Upon return to Earth animals displayed reduced rates of movement, indicating a functional defect. These results demonstrate that C. elegans muscle development is altered in response to spaceflight. This altered development occurs at the level of gene transcription and was observed in the presence of innervation, not simply in isolated cells. This important finding coupled with past observations of decreased levels of the same ...
https://jeb.biologists.org/content/209/16/3209.short
The human GRB2 and Drosophila Drk genes can functionally replace the Caenorhabditis elegans cell signaling gene sem-5<...
TY - JOUR. T1 - The human GRB2 and Drosophila Drk genes can functionally replace the Caenorhabditis elegans cell signaling gene sem-5. AU - Stern, M. J.. AU - Marengere, L. E.M.. AU - Daly, R. J.. AU - Lowenstein, E. J.. AU - Kokel, M.. AU - Batzer, A.. AU - Olivier, P.. AU - Pawson, T.. AU - Schlessinger, J.. PY - 1993/1/1. Y1 - 1993/1/1. N2 - Mutations in the Caenorhabditis elegans gene sem-5 affect cell signaling processes involved in guiding a class of cell migrations and inducing vulval cell fates. The sem-5 sequence encodes a protein comprised almost exclusively of SH2 and SH3 domains (SH, src homology region) that are found together in many signaling proteins and nonreceptor tyrosine kinases. A human protein, GRB2, was identified by its ability to associate with the activated human epidermal growth factor receptor (hEGFR). The GRB2 and Sem-5 proteins share an identical architecture of their SH2 and SH3 domains and 58% amino acid sequence identity. Here we demonstrate that GRB2 and a ...
https://research.monash.edu/en/publications/the-human-grb2-and-drosophila-drk-genes-can-functionally-replace-
Germ Granules Prevent Accumulation of Somatic Transcripts in the Adult Caenorhabditis elegans Germline | GeneticsGerm Granules Prevent Accumulation of Somatic Transcripts in the Adult Caenorhabditis elegans Germline | Genetics
The germ cells of multicellular organisms protect their developmental potential through specialized mechanisms. A shared feature of germ cells from worms to humans is the presence of nonmembrane-bound, ribonucleoprotein organelles called germ granules. Depletion of germ granules in Caenorhabditis elegans (i.e., P granules) leads to sterility and, in some germlines, expression of the neuronal transgene unc-119::gfp and the muscle myosin MYO-3. Thus, P granules are hypothesized to maintain germ cell totipotency by preventing somatic development, although the mechanism by which P granules carry out this function is unknown. In this study, we performed transcriptome and single molecule RNA-FISH analyses of dissected P granule-depleted gonads at different developmental stages. Our results demonstrate that P granules are necessary for adult germ cells to downregulate spermatogenesis RNAs and to prevent the accumulation of numerous soma-specific RNAs. P granule-depleted gonads that express the ...
http://www.genetics.org/content/206/1/163
Molecular genetics of the Caenorhabditis elegans heterochronic gene lin-14. | GeneticsMolecular genetics of the Caenorhabditis elegans heterochronic gene lin-14. | Genetics
We describe a general strategy for the genetic mapping in parallel of multiple restriction fragment length polymorphism (RFLP) loci. This approach allows the systematic identification for cloning of physical genetic loci within about 100 kb of any gene in Caenorhabditis elegans. We have used this strategy of parallel RFLP mapping to clone the heterochronic gene lin-14, which controls the timing and sequence of many C. elegans postembryonic developmental events. We found that of about 400 polymorphic loci in the C. elegans genome associated with the Tc1 family of repetitive elements, six are within 0.3 map unit of lin-14. The three closest lin-14-linked Tc1-containing restriction fragments were cloned and used to identify by hybridization an 830-kb region of contiguous cloned DNA fragments assembled from cosmid and yeast artificial chromosome libraries. A lin-14 intragenic recombinant that separated a previously cryptic lin-14 semidominant mutation from a cis-acting lin-14 suppressor mutation was ...
https://www.genetics.org/content/121/3/501?ijkey=d8e15512e1e5878d4a626946948270e65ea72c88&keytype2=tf_ipsecsha
The SH3 domain of UNC-89 (obscurin) interacts with paramyosin, a coiled-coil protein, in Caenorhabditis elegans muscleThe SH3 domain of UNC-89 (obscurin) interacts with paramyosin, a coiled-coil protein, in Caenorhabditis elegans muscle
rdf:RDF xmlns:dcterms=http://purl.org/dc/terms/ xmlns:dc=http://purl.org/dc/elements/1.1/ xmlns:rdf=http://www.w3.org/1999/02/22-rdf-syntax-ns# xmlns:bibo=http://purl.org/ontology/bibo/ xmlns:dspace=http://digital-repositories.org/ontologies/dspace/0.1.0# xmlns:foaf=http://xmlns.com/foaf/0.1/ xmlns:void=http://rdfs.org/ns/void# xmlns:xsd=http://www.w3.org/2001/XMLSchema# , ,rdf:Description rdf:about=https://kops.uni-konstanz.de/rdf/resource/123456789/34900, ,bibo:uri rdf:resource=https://kops.uni-konstanz.de/handle/123456789/34900/, ,dc:creator,Deehan, Kevin,/dc:creator, ,dc:creator,Wilson, Kristy J.,/dc:creator, ,dc:language,eng,/dc:language, ,dcterms:abstract xml:lang=eng,UNC-89 is a giant polypeptide located at the sarcomeric M-line of Caenorhabditis elegans muscle. The human homologue is obscurin. To understand how UNC-89 is localized and functions, we have been identifying its binding partners. Screening a yeast two-hybrid library revealed that UNC-89 interacts with ...
https://kops.uni-konstanz.de/handle/123456789/34900
The Caenorhabditis elegans protein SAS-5 forms large oligomeric assemblies critical for centriole formation - InfoscienceThe Caenorhabditis elegans protein SAS-5 forms large oligomeric assemblies critical for centriole formation - Infoscience
Centrioles are microtubule-based organelles crucial for cell division, sensing and motility. In Caenorhabditis elegans, the onset of centriole formation requires notably the proteins SAS-5 and SAS-6, which have functional equivalents across eukaryotic evolution. Whereas the molecular architecture of SAS-6 and its role in initiating centriole formation are well understood, the mechanisms by which SAS-5 and its relatives function is unclear. Here, we combine biophysical and structural analysis to uncover the architecture of SAS-5 and examine its functional implications in vivo. Our work reveals that two distinct self-associating domains are necessary to form higher-order oligomers of SAS-5: a trimeric coiled coil and a novel globular dimeric Implico domain. Disruption of either domain leads to centriole duplication failure in worm embryos, indicating that large SAS-5 assemblies are necessary for function in vivo. Rogala, Kacper B; Dynes, Nicola J; Hatzopoulos, Georgios N; Yan, Jun; Pong, Sheng Kai;
https://infoscience.epfl.ch/record/214462?ln=en
The Caenorhabditis elegans protein SAS-5 forms large oligomeric assemblies critical for centriole formation. - Oxford...
Centrioles are microtubule-based organelles crucial for cell division, sensing and motility. In Caenorhabditis elegans, the onset of centriole formation requires notably the proteins SAS-5 and SAS-6, which have functional equivalents across eukaryotic evolution. Whereas the molecular architecture of SAS-6 and its role in initiating centriole formation are well understood, the mechanisms by which SAS-5 and its relatives function is unclear. Here, we combine biophysical and structural analysis to uncover the architecture of SAS-5 and examine its functional implications in vivo. Our work reveals that two distinct self-associating domains are necessary to form higher-order oligomers of SAS-5: a trimeric coiled coil and a novel globular dimeric Implico domain. Disruption of either domain leads to centriole duplication failure in worm embryos, indicating that large SAS-5 assemblies are necessary for function in vivo.
https://www.cardioscience.ox.ac.uk/publications/525623
Determination of Life-Span in Caenorhabditis elegans by Four Clock Genes | ScienceDetermination of Life-Span in Caenorhabditis elegans by Four Clock Genes | Science
The nematode worm Caenorhabditis elegans is a model system for the study of the genetic basis of aging. Maternal-effect mutations in four genes-clk-1, clk-2, clk-3, and gro-1- interact genetically to determine both the duration of development and life-span. Analysis of the phenotypes of these mutants suggests the existence of a general physiological clock in the worm. Mutations in certain genes involved in dauer formation (an alternative larval stage induced by adverse conditions in which development is arrested) can also extend life-span, but the life extension of Clock mutants appears to be independent of these genes. The daf-2(e1370) clk-1(e2519) worms, which carry life-span-extending mutations from two different pathways, live nearly five times as long as wild-type worms.. ...
http://science.sciencemag.org/content/272/5264/1010
DIGITAL.CSIC: Protein kinase VRK-1 regulates cell invasion and EGL-17/FGF signaling in Caenorhabditis elegansDIGITAL.CSIC: Protein kinase VRK-1 regulates cell invasion and EGL-17/FGF signaling in Caenorhabditis elegans
The vaccinia-related kinases (VRKs) are highly conserved throughout the animal kingdom and phosphorylate several chromatin proteins and transcription factors. In early Caenorhabditis elegans embryos, VRK-1 is required for proper nuclear envelope formation. In this work, we present the first investigation of the developmental role of VRKs by means of a novel C. elegans vrk-1 mutant allele. We found that VRK-1 is essential in hermaphrodites for formation of the vulva, uterus, and utse and for development and maintenance of the somatic gonad and thus the germ line. VRK-1 regulates anchor cell polarity and the timing of anchor cell invasion through the basement membranes separating vulval and somatic gonadal cells during the L3 larval stage. VRK-1 is also required for proper specification and proliferation of uterine cells and sex myoblasts. Expression of the fibroblast growth factor-like protein EGL-17 and its receptor EGL-15 is reduced in vrk-1 mutants, suggesting that VRK-1 might act at least ...
https://digital.csic.es/handle/10261/17712
Genetic control of programmed cell death in the Caenorhabditis elegans hermaphrodite germline | DevelopmentGenetic control of programmed cell death in the Caenorhabditis elegans hermaphrodite germline | Development
Development of the nematode Caenorhabditis elegans is highly reproducible and the fate of every somatic cell has been reported. We describe here a previously uncharacterized cell fate in C. elegans: we show that germ cells, which in hermaphrodites can differentiate into sperm and oocytes, also undergo apoptotic cell death. In adult hermaphrodites, over 300 germ cells die, using the same apoptotic execution machinery (ced-3, ced-4 and ced-9) as the previously described 131 somatic cell deaths. However, this machinery is activated by a distinct pathway, as loss of egl-1 function, which inhibits somatic cell death, does not affect germ cell apoptosis. Germ cell death requires ras/MAPK pathway activation and is used to maintain germline homeostasis. We suggest that apoptosis eliminates excess germ cells that acted as nurse cells to provide cytoplasmic components to maturing oocytes.. ...
https://dev.biologists.org/content/126/5/1011?ijkey=f60a01edb8df79058159debe922cebfc75823ca8&keytype2=tf_ipsecsha
Novel cysteine-rich motif and homeodomain in the product of the Caenorhabditis elegans cell lineage gene lin-11Novel cysteine-rich motif and homeodomain in the product of the Caenorhabditis elegans cell lineage gene lin-11
The gene lin-11 is required for the asymmetric division of a vulval precursor cell type in the nematode Caenorhabditis elegans. Putative lin-11 complementary DNAs were sequenced and found to encode a protein that contains both a homeodomain and two tandem copies of a novel cysteine-rich motif: C-X2- …
https://pubmed.ncbi.nlm.nih.gov/1970421/?dopt=Abstract
Altered expression of an L1-specific, O-linked cuticle surface glycoprotein in mutants of the nematode Caenorhabditis elegans. ...Altered expression of an L1-specific, O-linked cuticle surface glycoprotein in mutants of the nematode Caenorhabditis elegans. ...
Mouse mAb M38 was used in indirect immunofluorescence experiments to detect a stage-specific antigen on the surface of the first larval stage (L1) of the free-living nematode Caenorhabditis elegans, and to detect alterations in the apparent expression of this antigen in two distinct classes of C. elegans mutants. In previously described srf-2 and srf-3 mutants (Politz S. M., M. T. Philipp, M. Estevez, P.J. OBrien, and K. J. Chin. 1990. Proc. Natl. Acad. Sci. USA. 87:2901-2905), the antigen is not detected on the surface of any stage. Conversely, in srf-(yj43) and other similar mutants, the antigen is expressed on the surface of the first through the fourth (L4) larval stages. To understand the molecular basis of these alterations, the antigen was characterized in gel immunoblotting experiments. After SDS-PAGE separation and transfer to nitrocellulose, M38 detected a protein antigen in extracts of wild-type L1 populations. The antigen was sensitive to digestion by Pronase and O-glycanase ...
http://jcb.rupress.org/content/115/5/1237.long
On the nature of undead cells in the nematode Caenorhabditis elegans | Philosophical Transactions of the Royal Society B:...On the nature of undead cells in the nematode Caenorhabditis elegans | Philosophical Transactions of the Royal Society B:...
During the course of normal embryonic and post-embryonic development, 131 cells in a Caenorhabditis elegans hermaphrodite undergo programmed cell death. Loss of function mutations in either of the genes ced-3 or ced-4 abolish cell deaths, enabling these undead cells to survive and be incorporated into the adult with no obvious deleterious consequences. Ultrastructural reconstructions have shown that undead cells exhibit many differentiated characteristics. Most of the reconstructed cells appeared to be neurons with all the characteristic features associated with such cells, such as processes, synaptic vesicles and presynaptic specializations. However, clear morphological differences were seen among the undead neurons, suggesting a diversity of cell type. One of the reconstructed cells was a rectal epithelial cell, which had displaced its lineal sister that normally functions in this role. Removal of the ability to undergo programmed cell death by mutation therefore reveals a diversity of ...
http://rstb.royalsocietypublishing.org/content/331/1261/263
The C. elegans Hox gene egl-5 is required for correct development of the hermaphrodite hindgut and for the response to rectal...
Members of the Hox gene family encode transcription factors that specify positional identity along the anterior-posterior axis of nearly all metazoans. One among the Caenorhabditis elegans Hox genes is egl-5. A deletion allele of egl-5 was isolated in a screen for animals which fail to develop swollen tails when exposed to the bacterial pathogen Microbacterium nematophilum. We show that compromised rectal development, which occurs as a result of loss of egl-5 function, results in a failure of rectal epithelial cells to express the ERK MAP kinase mpk-1, which was previously shown to mediate tail-swelling in response to bacterial infection. Tissue-specific rescue experiments demonstrated that egl-5 and mpk-1 act autonomously in rectal cells in the morphological response. The weak egl-5 allele (n1439), which does not compromise rectal development, fails to affect tail-swelling. We find that this allele carries an inserted repeat element approximately 13.8 kb upstream of the egl-5 open reading frame, which
https://www.neuroscience.ox.ac.uk/publications/202875
The role of insulin/IGF-like signaling in C. elegans longevity and aging. | Lewis-Sigler InstituteThe role of insulin/IGF-like signaling in C. elegans longevity and aging. | Lewis-Sigler Institute
Aging is characterized by general physiological decline over time. A hallmark of human senescence is the onset of various age-related afflictions including neurodegeneration, cardiovascular disease and cancer. Although environmental and stochastic factors undoubtedly contribute to the increased incidence of disease with age, recent studies suggest that intrinsic genetic determinants govern both life span and overall health. Current aging research aims at achieving the longevity dividend, in which life span extension in humans is accomplished with a concomitant increase in the quality of life (Olshansky et al., 2007). Significant progress has been made using model organisms, especially the nematode worm Caenorhabditis elegans, to delineate the genetic and biochemical pathways involved in aging to identify strategies for therapeutic intervention in humans. In this review, we discuss how C. elegans has contributed to our understanding of insulin signaling and aging. ...
https://lsi.princeton.edu/role-insulinigf-signaling-c-elegans-longevity-and-aging
Serotonin-deficient Mutants and Male Mating Behavior in the Nematode Caenorhabditis Elegans - PubMedSerotonin-deficient Mutants and Male Mating Behavior in the Nematode Caenorhabditis Elegans - PubMed
Defining a behavior that requires the function of specific neurons in the free-living nematode Caenorhabditis elegans can allow one to screen for mutations that disrupt the specification or function of those neurons. We identified serotonin-immunoreactive neurons required for tail curling or turnin …
https://pubmed.ncbi.nlm.nih.gov/8254383/
An evidence-based approach to identify aging-related genes in Caenorhabditis elegans | BMC Bioinformatics | Full TextAn evidence-based approach to identify aging-related genes in Caenorhabditis elegans | BMC Bioinformatics | Full Text
Extensive studies have been carried out on Caenorhabditis elegans as a model organism to elucidate mechanisms of aging and the effects of perturbing known aging-related genes on lifespan and behavior. This research has generated large amounts of experimental data that is increasingly difficult to integrate and analyze with existing databases and domain knowledge. To address this challenge, we demonstrate a scalable and effective approach for automatic evidence gathering and evaluation that leverages existing experimental data and literature-curated facts to identify genes involved in aging and lifespan regulation in C. elegans. We developed a semantic knowledge base for aging by integrating data about C. elegans genes from WormBase with data about 2005 human and model organism genes from GenAge and 149 genes from GenDR, and with the Bio2RDF network of linked data for the life sciences. Using HyQue (a Semantic Web tool for hypothesis-based querying and evaluation) to interrogate this knowledge base, we
https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-015-0469-4
IP3 signalling regulates exogenous RNAi in Caenorhabditis elegans | EMBO Reports
RNA interference (RNAi) is a widespread and widely exploited phenomenon which has potential as a strategy for both the treatment of disease and pest control. RNAi results in down‐regulation of a specific gene in response to the production of small interfering RNAs (siRNAs). RNAi is one of a family of processes mediated by small non‐coding RNAs [1], [2]. In Caenorhabditis elegans, and in a number of other organisms, RNAi is systemic so that the introduction of dsRNA into one tissue triggers gene silencing in other tissues [3], [4], [5], [6], [7]. Furthermore, systemic RNAi enables C. elegans and other organisms to exhibit environmental RNAi [5]. For example, feeding C. elegans on bacteria expressing dsRNA initiates a widespread RNAi response [8], [9]. Studies in C. elegans and other organisms have provided mechanistic insights into RNAi [4], [10], [11], [12], [13], although the role of exogenous RNAi in the normal life of C. elegans and other animals remains unclear [14].. Whilst C. elegans ...
http://embor.embopress.org/content/early/2015/01/21/embr.201439585
Axonal guidance defects in a Caenorhabditis elegans mutant reveal cell-extrinsic determinants of neuronal morphology.
Mutations in the gene unc-53 of Caenorhabditis elegans result in behavioral and anatomical abnormalities. Immunocytochemistry and electron microscopy revealed neuroanatomical defects in all main longitudinal nervous tracts. Whole tracts were found to
http://www.biomedsearch.com/nih/Axonal-guidance-defects-in-Caenorhabditis/8410186.html
Caenorhabditis elegans MIG-13 protein Summary Report | CureHunterCaenorhabditis elegans MIG-13 protein Summary Report | CureHunter
Caenorhabditis elegans MIG-13 protein: required for positioning of Q neuroblasts and their descendents along the anteroposterior axis; isolated from Caenorhabditis elegans; amino acid sequence in first source; GenBAnk AF150958
http://www.curehunter.com/public/keywordSummaryC120452-Caenorhabditis-elegans-MIG-13-protein.do
C. elegans** homologs of insect clock proteins: a tale of many stories - Institutional Repository University of AntwerpC. elegans** homologs of insect clock proteins: a tale of many stories - Institutional Repository University of Antwerp
As a consequence of the Earths axial rotation, organisms display daily recurring rhythms in behavior and biochemical properties, such as hormone titers. The neuronal system controlling such changes is best studied in the fruit fly Drosophila melanogaster. In the nematode worm Caenorhabditis elegans, most homologs of these genes function in the heterochronic pathway controlling the (timing of) developmental events. Recent data indicate that in the worm at least one of the genes involved in developmental timing is also active in circadian rhythm control, thereby opening up new perspectives on a central (neuronal) timer interfering with many processes. Also, new neuropeptidergic clock homologs have been identified in nematodes, supporting the idea of a broad range of clock-regulated targets. We will describe the current knowledge on homologous clock genes in C. elegans with a focus on the recently discovered pigment dispersing factor gene homologs. Similarities between developmental and daily ...
https://repository.uantwerpen.be/link/irua/106368
The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans - NASA/ADSThe 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans - NASA/ADS
The C. elegans heterochronic gene pathway consists of a cascade of regulatory genes that are temporally controlled to specify the timing of developmental events. Mutations in heterochronic genes cause temporal transformations in cell fates in which stage-specific events are omitted or reiterated. Here we show that let-7 is a heterochronic switch gene. Loss of let-7 gene activity causes reiteration of larval cell fates during the adult stage, whereas increased let-7 gene dosage causes precocious expression of adult fates during larval stages. let-7 encodes a temporally regulated 21-nucleotide RNA that is complementary to elements in the 3 untranslated regions of the heterochronic genes lin-14, lin-28, lin-41, lin-42 and daf-12, indicating that expression of these genes may be directly controlled by let-7. A reporter gene bearing the lin-41 3 untranslated region is temporally regulated in a let-7-dependent manner. A second regulatory RNA, lin-4, negatively regulates lin-14 and lin-28 through RNA-RNA
https://ui.adsabs.harvard.edu/abs/2000Natur.403..901R
Engulfment genes cooperate with ced-3 to promote cell death in Caenorhabditis elegans - CSHL Scientific Digital RepositoryEngulfment genes cooperate with ced-3 to promote cell death in Caenorhabditis elegans - CSHL Scientific Digital Repository
Genetic studies have identified over a dozen genes that function in programmed cell death (apoptosis) in the nematode Caenorhabditis elegans(1-3). Although the ultimate effects on cell survival or engulfment of mutations in each cell death gene have been extensively described, much less is known about how these mutations affect the kinetics of death and engulfment, or the interactions between these two processes. We have used four-dimensional-Nomarski time-lapse video microscopy to follow in detail how cell death genes regulate the extent and kinetics of apoptotic cell death and removal in the early C. elegans embryo. Here we show that blocking engulfment enhances cell survival when cells are subjected to weak pro-apoptotic signals. Thus, genes that mediate corpse removal can also function to actively kill cells.. ...
http://repository.cshl.edu/id/eprint/29251/
foxo;Pi3K92E
Abstract The insulin/insulin-like growth factor-like signaling (IIS) pathway in metazoans has evolutionarily conserved roles in growth control, metabolic homeostasis, stress responses, reproduction, and lifespan. Genetic manipulations that reduce IIS in the nematode worm Caenorhabditis elegans, the fruit fly Drosophila melanogaster, and the mouse have been shown not only to produce substantial increases in lifespan but also to ameliorate several age-related diseases. In C. elegans, the multitude of phenotypes produced by the reduction in IIS are all suppressed in the absence of the worm FOXO transcription factor, DAF-16, suggesting that they are all under common regulation. It is not yet clear in other animal models whether the activity of FOXOs mediate all of the physiological effects of reduced IIS, especially increased lifespan. We have addressed this issue by examining the effects of reduced IIS in the absence of dFOXO in Drosophila, using a newly generated null allele of dfoxo. We found ...
http://synergistic.aging-research.group/details/3274/
CYP-13A12 of the nematode Caenorhabditis elegans is a PUFA-epoxygenase involved in behavioural response to reoxygenation |...CYP-13A12 of the nematode Caenorhabditis elegans is a PUFA-epoxygenase involved in behavioural response to reoxygenation |...
A specific behavioural response of Caenorhabditis elegans, the rapid increase of locomotion in response to anoxia/reoxygenation called the O2-ON response, has been used to model key aspects of ischaemia/reperfusion injury. A genetic suppressor screen demonstrated a direct causal role of CYP (cytochrome P450)-13A12 in this response and suggested that CYP-eicosanoids, which in mammals influence the contractility of cardiomyocytes and vascular smooth muscle cells, might function in C. elegans as specific regulators of the body muscle cell activity. In the present study we show that co-expression of CYP-13A12 with the NADPH-CYP-reductase EMB-8 in insect cells resulted in the reconstitution of an active microsomal mono-oxygenase system that metabolized EPA (eicosapentaenoic acid) and also AA (arachidonic acid) to specific sets of regioisomeric epoxy and hydroxy derivatives. The main products included 17,18-EEQ (17,18-epoxyeicosatetraenoic acid) from EPA and 14,15-EET (14,15-epoxyeicosatrienoic acid) ...
http://www.biochemj.org/content/464/1/61
EFF-1 fusogen promotes phagosome sealing during cell process clearance in Caenorhabditis elegans | Nature Cell BiologyEFF-1 fusogen promotes phagosome sealing during cell process clearance in Caenorhabditis elegans | Nature Cell Biology
Phagocytosis of dying cells is critical in development and immunity1-3. Although proteins for recognition and engulfment of cellular debris following cell death are known4,5, proteins that directly mediate phagosome sealing are uncharacterized. Furthermore, whether all phagocytic targets are cleared using the same machinery is unclear. Degeneration of morphologically complex cells, such as neurons, glia and melanocytes, produces phagocytic targets of various shapes and sizes located in different microenvironments6,7. Thus, such cells offer unique settings to explore engulfment programme mechanisms and specificity. Here, we report that dismantling and clearance of a morphologically complex Caenorhabditis elegans epithelial cell requires separate cell soma, proximal and distal process programmes. Similar compartment-specific events govern the elimination of a C. elegans neuron. Although canonical engulfment proteins drive cell soma clearance, these are not required for process removal. We find that EFF-1,
https://www.nature.com/articles/s41556-018-0068-5?error=cookies_not_supported&code=a774400d-5e29-4d09-bcd0-194912231f5a
Genetic and molecular analysis of a Caenorhabditis elegans beta-tubulin that conveys benzimidazole sensitivity. | JCBGenetic and molecular analysis of a Caenorhabditis elegans beta-tubulin that conveys benzimidazole sensitivity. | JCB
Benzimidazole anti-microtubule drugs, such as benomyl, induce paralysis and slow the growth of the nematode Caenorhabditis elegans. We have identified 28 mutations in C. elegans that confer resistance to benzimidazoles. All resistant mutations map to a single locus, ben-1. Virtually all these mutations are genetically dominant. Molecular cloning and DNA sequence analysis established that ben-1 encodes a beta-tubulin. Some resistant mutants are completely deleted for the ben-1 gene. Since the deletion strains appear to be fully resistant to the drugs, the ben-1 product appears to be the only benzimidazole-sensitive beta-tubulin in C. elegans. Furthermore, since animals lacking ben-1 are viable and coordinated, the ben-1 beta-tubulin appears to be nonessential for growth and movement. The ben-1 function is likely to be redundant in the nematode genome. ...
http://jcb.rupress.org/content/109/6/2993
A semi-automated system based on level sets and invariant spatial interrelation shape features for Caenorhabditis elegans...A semi-automated system based on level sets and invariant spatial interrelation shape features for Caenorhabditis elegans...
Caenorhabditis elegans shares several molecular and physiological homologies with humans and thus plays a key role in studying biological processes. As a consequence, much progress has been made in automating the analysis of C. elegans. However, there is still a strong need to achieve more progress in automating the analysis of static images of adult worms. In this paper, a three-phase semi-automated system has been proposed. As a first phase, a novel segmentation framework, based on variational level sets and local pressure force function, has been introduced to handle effectively images corrupted with intensity inhomogeneity. Then, a set of robust invariant symbolic features for high-throughput screening of image-based C. elegans phenotypes are extracted. Finally, a classification model is applied to discriminate between the different subsets. The proposed system demonstrates its effectiveness in measuring morphological phenotypes in individual worms of C. elegans.. ...
http://wrap.warwick.ac.uk/96383/
VAB-10 spectraplakin acts in cell and nuclear migration in Caenorhabditis elegans | DevelopmentVAB-10 spectraplakin acts in cell and nuclear migration in Caenorhabditis elegans | Development
Cytoskeletal regulation is important in cell migration. The Caenorhabditis elegans gonadal distal tip cells (DTCs) offer a simple model with which to investigate the mechanism of cell migration in organogenesis. Here, we report that one of the spectraplakin isoforms, VAB-10B1, plays an essential role in cell and nuclear migration of DTCs by regulating the actin and microtubule (MT) cytoskeleton. In the vab-10(tk27) mutant, which lacks VAB-10B1, alignment of filamentous (F)-actin and MTs was weakly and severely disorganized, respectively, which resulted in a failure to translocate the DTC nucleus and a premature termination of DTC migration. An MT growing-tip marker, EBP-2-GFP, revealed that polarized outgrowth of MTs towards the nuclei of migrating DTCs was strikingly impaired in tk27 animals. A vab-10 mini-gene encoding only the actin- and MT-binding domains significantly rescued the gonadal defects, suggesting that VAB-10B1 has a role in linking actin and MT filaments. These results suggest ...
http://dev.biologists.org/content/early/2011/08/10/dev.059568
C. elegans ced-13 can promote apoptosis and is induced in response to DNA damage.C. elegans ced-13 can promote apoptosis and is induced in response to DNA damage.
Amino Acid Sequence, Animals, Apoptosis/*physiology, Apoptosis Regulatory Proteins, Caenorhabditis/genetics, Caenorhabditis elegans/embryology/genetics/*physiology, Caenorhabditis elegans Proteins/genetics/metabolism/*physiology, DNA/genetics/radiation effects, DNA Damage, Gene Deletion, Gene Expression Regulation; Developmental/radiation effects, Heat-Shock Proteins/genetics, Models; Biological, Molecular Sequence Data, Mutation, Proto-Oncogene Proteins/genetics/metabolism, Repressor Proteins/genetics, Sequence Homology; Amino Acid, Tumor Suppressor Protein p53/genetics/physiology, X-Rays ...
http://umu.diva-portal.org/smash/record.jsf?pid=diva2:157623
Fermented Brown Sugar Residue Prolongs the Caenorhabditis elegans Lifespan via DAF-16Fermented Brown Sugar Residue Prolongs the Caenorhabditis elegans Lifespan via DAF-16
Purification of biomass ethanol from the products of brown sugar yeast-fermentation produces a large amount of residue. This fermentation residue contains abundant brown sugar-derived nutrients and is mainly used as compost or livestock feed. However, the in vivo physiological effects of oral residue ingestion are not known. The purpose of this study was to elucidate the physiological action and molecular mechanism of fermented brown sugar residue in nematode stress tolerance, aging, and lifespan using Caenorhabditis elegans. Fermented brown sugar residue was divided into two layers, supernatant and precipitate, and each was given to nematodes. Analysis of motility and survival rate under thermal stress revealed reduced mobility and increased survival rate following treatment with fermented brown sugar residue. The survival rate of nematodes under 1% H2O2 was markedly increased by the residue and mitochondrial membrane depolarization was induced and mitochondrial radical oxygen species levels increased.
https://file.scirp.org/Html/1-2702167_79004.htm
A compendium of Caenorhabditis elegans RNA binding proteins predicts e by Alex M. Tamburino, Sean P. Ryder et al.A compendium of Caenorhabditis elegans RNA binding proteins predicts e by Alex M. Tamburino, Sean P. Ryder et al.
Gene expression is regulated at multiple levels, including transcription and translation, as well as mRNA and protein stability. Although systems-level functions of transcription factors and microRNAs are rapidly being characterized, few studies have focused on the posttranscriptional gene regulation by RNA binding proteins (RBPs). RBPs are important to many aspects of gene regulation. Thus, it is essential to know which genes encode RBPs, which RBPs regulate which gene(s), and how RBP genes are themselves regulated. Here we provide a comprehensive compendium of RBPs from the nematode Caenorhabditis elegans (wRBP1.0). We predict that as many as 887 (4.4%) of C. elegans genes may encode RBPs ~250 of which likely function in a gene-specific manner. In addition, we find that RBPs, and most notably gene-specific RBPs, are themselves enriched for binding and modification by regulatory proteins, indicating the potential for extensive regulation of RBPs at many different levels. wRBP1.0 will provide a
https://escholarship.umassmed.edu/sysbio_pubs/23/
The WAVE/SCAR complex promotes polarized cell movements and actin enrichment in epithelia during C. elegans embryogenesis. -...
The WAVE/SCAR complex promotes actin nucleation through the Arp2/3 complex, in response to Rac signaling. We show that loss of WVE-1/GEX-1, the only C. elegans WAVE/SCAR homolog, by genetic mutation or by RNAi, has the same phenotype as loss of GEX-2/Sra1/p140/PIR121, GEX-3/NAP1/HEM2/KETTE, or ABI-1/ABI, the three other components of the C. elegans WAVE/SCAR complex. We find that the entire WAVE/SCAR complex promotes actin-dependent events at different times and in different tissues during development. During C. elegans embryogenesis loss of CED-10/Rac1, WAVE/SCAR complex components, or Arp2/3 blocks epidermal cell migrations despite correct epidermal cell differentiation. 4D movies show that this failure occurs due to decreased membrane dynamics in specific epidermal cells. Unlike myoblasts in Drosophila, epidermal cell fusions in C. elegans can occur in the absence of WAVE/SCAR or Arp2/3. Instead we find that subcellular enrichment of F-actin in epithelial tissues requires the Rac-WAVE/SCAR-Arp2/3
https://www.hal.inserm.fr/inserm-00351005
Regulation of anchor cell invasion and uterine cell fates by the egl-43 Evi-1 proto-oncogene in Caenorhabditis elegans.
Cell invasion is a tightly controlled process occurring during development and tumor progression. The nematode Caenorhabditis elegans serves as a genetic model to study cell invasion during normal development. In the third larval stage, the anchor ce
http://www.biomedsearch.com/nih/Regulation-anchor-cell-invasion-uterine/17573066.html
Aging and resistance to oxidative damage in Caenorhabditis elegans | PNASAging and resistance to oxidative damage in Caenorhabditis elegans | PNAS
The dauer larva state and the age-1 mutation, both of which extend life-span in Caenorhabditis elegans, were tested for hyperresistance to cellular damage that may be relevant to aging. The age-1 strain TJ401 displayed hyperresistance to oxidative stress relative to its parental strain. The activities of two enzymes that protect cells from oxidative damage, superoxide dismutase (SOD) and catalase, showed an age-dependent increase in mutant animals, which was not seen in the parental strain. These increases in activities paralleled the time course of the hyperresistance. The results are consistent with the age-1 gene product functioning as a negative regulator of SOD and catalase activities. In wild-type and age-1 dauer larvae, elevated levels of SOD activity, but not of catalase activity, were present when compared with young adults. The common increase in SOD activity prompted cloning the C. elegans Cu/Zn SOD gene. Its position on the physical map of the genome was in the region to which the ...
https://www.pnas.org/content/90/19/8905?ijkey=4bab03f6e1907757fea959dde092b37a41889ee8&keytype2=tf_ipsecsha
The CeCDC-14 phosphatase is required for cytokinesis in the Caenorhabditis elegans embryo | Journal of Cell Biology |...The CeCDC-14 phosphatase is required for cytokinesis in the Caenorhabditis elegans embryo | Journal of Cell Biology |...
A search of the C. elegans genome for potential homologues of the yeast MEN/SIN genes revealed several candidate genes, although for some of these genes the sequence similarities were only limited and for TEM1, CDC15, and BFA1 no putative homologues could be identified (Table I). All candidate genes were tested in C. elegans for a possible function in a hypothetical MEN/SIN-like regulatory network, using RNAi to deplete the corresponding products. For depletion of putative MEN/SIN gene products, both RNAi feeding and injection methods (Montgomery and Fire, 1998; Timmons et al., 2001) were tried to maximize the probability of functional inactivation. The results of these experiments are summarized in Table I. A priori, we had expected that depletion of a gene product required for the regulation of mitotic exit or the onset of cytokinesis should result in embryonic lethality. However, of all components tested, only the depletion of the C. elegans homologue of the budding yeast Cdc14p phosphatase ...
https://rupress.org/jcb/article/158/5/901/32809/The-CeCDC-14-phosphatase-is-required-for
Reliable CRISPR/Cas9 Genome Engineering in Caenorhabditis elegans Using a Single Efficient sgRNA and an Easily Recognizable...Reliable CRISPR/Cas9 Genome Engineering in Caenorhabditis elegans Using a Single Efficient sgRNA and an Easily Recognizable...
The pace of technical developments allowing the direct manipulation of genome sequences has seen a marked acceleration in recent years with the emergence of RNA-targeted nucleases derived from bacterial immune systems (Doudna and Charpentier 2014; Zetsche et al. 2015). In particular, the binary system relying on the Streptococcus pyogenes Cas9 endonuclease targeted by CRISPR (clustered, regularly interspaced, short, palindromic repeat) RNAs has been successfully used to generate point mutations, deletion, or DNA insertions in an ever-growing number of experimental systems. S. pyogenes CRISPR/Cas9 has been adapted early on in the model nematode Caenorhabditis elegans (Friedland et al. 2013; Dickinson et al. 2013; Chen et al. 2013; Frøkjær-Jensen 2013; Dickinson and Goldstein 2016). Previously, heritable genome engineering could only be achieved in C. elegans by remobilizing a Drosophila Mos1 transposon, which could be inserted and excised in the germline (Robert and Bessereau 2007; ...
http://www.g3journal.org/content/7/5/1429
The Caenorhabditis elegans homolog of the Opitz syndrome gene, madd-2/Mid1, regulates anchor cell invasion during vulval...The Caenorhabditis elegans homolog of the Opitz syndrome gene, madd-2/Mid1, regulates anchor cell invasion during vulval...
Mutations in the human Mid1 gene cause Opitz G/BBB syndrome, which is characterized by various midline closure defects. The Caenorhabditis elegans homolog of Mid1, madd-2, positively regulates signaling by the unc-40 Netrin receptor during the extension of muscle arms to the midline and in axon guidance and branching. During uterine development, a specialized cell called anchor cell (AC) breaches the basal laminae separating the uterus from the epidermis and invades the underlying vulval tissue. AC invasion is guided by an UNC-6 Netrin signal from the ventral nerve cord and an unknown guidance signal from the vulval cells. Using genetic epistasis analysis, we show that madd-2 regulates AC invasion downstream of or in parallel with the Netrin signaling pathway. Measurements of AC shape, polarity and dynamics indicate that MADD-2 prevents the formation of ectopic AC protrusions in the absence of guidance signals. We propose that MADD-2 represses the intrinsic invasive capacity of the AC, while the ...
http://www.zora.uzh.ch/id/eprint/69277/
The C. elegans Male:Postembryonic Development of Nongonadal StructuresThe C. elegans Male:Postembryonic Development of Nongonadal Structures
Brenner, S. (1974). The genetics of Caenorhabditis elegans. Genetics 77, 71-94.. Chitwood, B. G., and Chitwood, M. B. (1974). Introduction to Nematology. University Park Press, Baltimore.. Hodgkin, J. A. (1974). Genetic and Anatomical Aspects of the Caenorhabditis elegans Male, Ph.D. thesis. University of Cambridge, Cambridge, England.. Hodgkin, J. A., and Brenner, S. (1977). Mutations causing transformation of sexual phenotype in the nematode Caenorhabditis elegans. Genetics 86, 275-287.. Kimble, J., and Hirsh, D. (1979). The Postembryonic cell lineages of the hermaphrodite and male gonads in Caenorhabditis elegans. Develop. Biol. 70, 396-417.. Klass, M., Wolf, N., and Hirsh, D. (1976). Development of the male reproductive system and sexual transformation in the nematode Caenorhabditis elegans. Develop. Biol. 52, 1-18.. Seligman, I. M., Filshie, B. K., Doy, F. A., and Crossley, A. C. (1975). Hormonal control of mor-phogenetic cell death of the wing hypodermis in Lucilia cuprina. Tissue Cell ...
http://wormatlas.org/ver1/Sulston%20postembmale_1980/references.html
Developmental regulation of Caenorhabditis elegans DNA topoisomerase I expression<...Developmental regulation of Caenorhabditis elegans DNA topoisomerase I expression<...
TY - JOUR. T1 - Developmental regulation of Caenorhabditis elegans DNA topoisomerase I expression. AU - Jang, Yeon Joo. AU - Park, Hyungki. AU - Lee, Junho. AU - Koo, Hyeon Sook. PY - 1998/5/31. Y1 - 1998/5/31. N2 - The developmental regulation of Caenorhabditis elegans DNA topoisomerase I expression was examined using synchronized Caenorhabditis elegans cultures. Variations of the relative mRNA and protein levels of the enzyme during their development were measured by Northern and Western analyses, respectively. The mRNA level was the highest at the embryonic stage, decreasing rapidly to the one tenth level at the L1 stage, and then increasing by a few fold at the L4 and young adult stages. The protein level was the highest at the L1 stage, with gradual decreasing at the following stages until it showed a slight increase at the young adult stage. Based on our results of the expressional regulation, the possible roles of DNA topoisomerase I in the development of C. elegans are discussed.. AB - ...
https://yonsei.pure.elsevier.com/en/publications/developmental-regulation-of-caenorhabditis-elegans-dna-topoisomer
The Caenorhabditis elegans AMP-activated protein kinase AAK-2 is phosphorylated by LKB1 and is required for resistance to...The Caenorhabditis elegans AMP-activated protein kinase AAK-2 is phosphorylated by LKB1 and is required for resistance to...
TY - JOUR. T1 - The Caenorhabditis elegans AMP-activated protein kinase AAK-2 is phosphorylated by LKB1 and is required for resistance to oxidative stress and for normal motility and foraging behavior. AU - Lee, Hyojin. AU - Jeong, Soo Cho. AU - Lambacher, Nils. AU - Lee, Jieun. AU - Lee, Se Jin. AU - Tae, Hoon Lee. AU - Gartner, Anton. AU - Koo, Hyeon Sook. PY - 2008/5/30. Y1 - 2008/5/30. N2 - AAK-2 is one of two α isoforms of the AMP-activated protein kinase in Caenorhabditis elegans and is involved in life span maintenance, stress responses, and germ cell cycle arrest upon dauer entry. We found that AAK-2 was phosphorylated at threonine 243 in response to paraquat treatment and that this phosphorylation depends on PAR-4, the C. elegans LKB1 homologue. Both aak-2 mutation and par-4 knockdown increased the sensitivity of C. elegans worms to paraquat, and the double deficiency did not further increase sensitivity, indicating that aak-2 and par-4 act in a linear pathway. Both mutations also ...
https://yonsei.pure.elsevier.com/en/publications/the-caenorhabditis-elegans-amp-activated-protein-kinase-aak-2-is-
Origin, properties, and regulated expression of multiple mRNAs encoded by the protein kinase C1 gene of Caenorhabditis elegans<...Origin, properties, and regulated expression of multiple mRNAs encoded by the protein kinase C1 gene of Caenorhabditis elegans<...
TY - JOUR. T1 - Origin, properties, and regulated expression of multiple mRNAs encoded by the protein kinase C1 gene of Caenorhabditis elegans. AU - Land, Marianne. AU - Islas-Trejo, Alma. AU - Rubin, Charles S.. N1 - Copyright: Copyright 2005 Elsevier B.V., All rights reserved.. PY - 1994/5/20. Y1 - 1994/5/20. N2 - Recently, we cloned and characterized cDNA encoding a novel, protein kinase C (designated PKC1B) from Caenorhabditis elegans. PKC1B (707 amino acid residues) is a developmentally regulated, calcium-independent kinase that is expressed exclusively in sensory neurons and related interneurons. We have now discovered a mechanism by which a second, distinct mRNA (PKC1A mRNA) with increased protein coding potential is generated from the C. elegans PKC1 gene. PKC1A mRNA is produced in a process that involves the utilization of an alternative, distal promoter, the incorporation of two unique exons into the mRNA, and alternative cis/trans splicing. Diversity among PKC1 gene transcripts is ...
https://einstein.pure.elsevier.com/en/publications/origin-properties-and-regulated-expression-of-multiple-mrnas-enco-2
Interactions between innexins UNC-7 and UNC-9 mediate electrical synapse specificity in the Caenorhabditis elegans locomotory...Interactions between innexins UNC-7 and UNC-9 mediate electrical synapse specificity in the Caenorhabditis elegans locomotory...
Approximately 10% of Caenorhabditis elegans nervous system synapses are electrical, that is, gap junctions composed of innexins. The locomotory nervous system consists of several pairs of interneurons and three major classes of motor neurons, all with stereotypical patterns of connectivity that include gap junctions. Mutations in the two innexin genes unc-7 and unc-9 result in identical uncoordinated movement phenotypes, and their respective gene products were investigated for their contribution to electrical synapse connectivity. unc-7 encodes three innexin isoforms. Two of these, UNC-7S and UNC-7SR, are functionally equivalent and play an essential role in coordinated locomotion. UNC-7S and UNC-7SR are widely expressed and co-localize extensively with green fluorescent protein-tagged innexin UNC-9 in the ventral and dorsal nerve cords. A subset of UNC-7S/SR expression visualizes gap junctions formed between the AVB forward command interneurons and their B class motor neuron partners. Experiments
https://neuraldevelopment.biomedcentral.com/articles/10.1186/1749-8104-4-16
Two cytochrome P450s in Caenorhabditis elegans are essential for the organization of eggshell, correct execution of meiosis and...
The role of lipids in the process of embryonic development of Caenorhabditis elegans is still poorly understood. Cytochrome P450s, a class of lipid-modifying enzymes, are good candidates to be involved in the production or degradation of lipids essential for development. We investigated two highly similar cytochrome P450s in C. elegans, cyp-31A2 and cyp-31A3, that are homologs of the gene responsible for Bietti crystalline corneoretinal dystrophy in humans. Depletion of both cytochromes either by RNAi or using a double deletion mutant, led to the failure of establishing the correct polarity of the embryo and to complete the extrusion of the polar bodies during meiosis. In addition, the egg became osmotic sensitive and permeable to dyes. The phenotype of cyp-31A2 or cyp-31A3 is very similar to a class of mutants that have polarization and osmotic defects (POD), thus the genes were renamed to pod-7 and pod-8, respectively. Electron microscopic analysis demonstrated that the activity of pod-7/pod-8 ...
http://vivo.mblwhoilibrary.org/display/publication48720
The Caenorhabditis elegans GATA factor elt-1 is essential for differentiation and maintenance of hypodermal seam cells and for...The Caenorhabditis elegans GATA factor elt-1 is essential for differentiation and maintenance of hypodermal seam cells and for...
The elt-1 RNAi phenotype provides a useful insight into the function of seam cells during postembryonic development. The loss of alae in the adult cuticle confirms the role of seam cells in producing this structure, which has previously been shown by laser ablation studies (Singh and Sulston, 1978). The apparently normal appearance of the underlying cuticle is also consistent with these previous studies and presumably this is derived from the dorsal/ventral hypodermis. RNAi of elt-1, applied during larval development, has a severe effect on the integrity of adult worms within a few hours of the L4-adult moult. Adult hermaphrodites show a `burst-vulva phenotype, in which the uterus herneates through the vulva. This is likely to be a direct consequence of seam-cell loss because the lateral seam anchors the vulval and uterine cells in position by virtue of the utse cell connection (Michaux et al., 2001; Newman et al., 2000; Sharma-Kishore et al., 1999). This hypothesis is supported by the ...
http://jcs.biologists.org/content/118/24/5709
QTL mapping for Caenorhabditis elegans survivorship in response to Escherichia coli and Stenotrophomonas maltophiliaQTL mapping for Caenorhabditis elegans survivorship in response to Escherichia coli and Stenotrophomonas maltophilia
Caenorhabditis elegans are free-living bacterivorous nematodes that naturally consume bacteria as food source. As an excellent genetic model, C. elegans has proven to be a successful system to study innate immune responses to human pathogens, which resulted in identification of many evolutionarily conserved defense pathways. Most of these studies examined innate immune pathway mutants in a single genetic background in response to monoculture of human pathogens that worms might not necessarily encounter in the wild. While this has led to the successful genetic dissection of these defense pathways, in order to fully understand their biological functions, the relevant ecological and evolutionary context needs to be taken into account. The bacterial environment C. elegans naturally encounter is likely to be highly heterogeneous. While many bacteria are mainly considered as dietary resource for worms, some could be potential pathogens. Worms thus constantly face the challenge to defend against the ...
http://krex.k-state.edu/dspace/handle/2097/32624
Hydrogen sulfide is an endogenous regulator of aging in Caenorhabditis elegans | DR-NTUHydrogen sulfide is an endogenous regulator of aging in Caenorhabditis elegans | DR-NTU
Aims: To investigate the role of endogenous hydrogen sulfide (H2S) in the control of aging and healthspan of Caenorhabditis elegans. Results: We show that the model organism, C. elegans, synthesizes H2S. Three H2S-synthesizing enzymes are present in C. elegans, namely cystathionine γ lyase (CSE), cystathionine β synthetase, and 3-mercaptopyruvate transferase (MPST or 3-MST). Genetic deficiency of mpst-1 (3-MST orthologue 1), but not cth-2 (CSE orthologue), reduced the lifespan of C. elegans. This effect was reversed by a pharmacological H2S donor (GYY4137). GYY4137 also reduced detrimental age-dependent changes in a range of physiological indices, including pharyngeal contraction and defecation. Treatment of C. elegans with GYY4137 increased the expression of several age-related, stress response, and antioxidant genes, whereas MitoSOX Red fluorescence, indicative of reactive oxygen species generation, was increased in mpst-1 knockouts and decreased by GYY4137 treatment. GYY4137 additionally ...
https://dr.ntu.edu.sg/handle/10220/19282
Mechanism of transport of IFT particles in C. elegans cilia by the concerted action of kinesin-II and OSM-3 motors<...
TY - JOUR. T1 - Mechanism of transport of IFT particles in C. elegans cilia by the concerted action of kinesin-II and OSM-3 motors. AU - Pan, Xiaoyu. AU - Ou, Guangshuo. AU - Civelekoglu-Scholey, Gul. AU - Blacque, Oliver E.. AU - Endres, Nicholas F.. AU - Tao, Li. AU - Mogilner, Alex. AU - Leroux, Michel R.. AU - Vale, Ronald D.. AU - Scholey, Jonathan M.. PY - 2006/9/25. Y1 - 2006/9/25. N2 - The assembly and function of cilia on Caenorhabditis elegans neurons depends on the action of two kinesin-2 motors, heterotrimeric kinesin-II and homodimeric OSM-3-kinesin, which cooperate to move the same intraflagellar transport (IFT) particles along microtubule (MT) doublets. Using competitive in vitro MT gliding assays, we show that purified kinesin-II and OSM-3 cooperate to generate movement similar to that seen along the cilium in the absence of any additional regulatory factors. Quantitative modeling suggests that this could reflect an alternating action mechanism, in which the motors take turns to ...
https://nyu-staging.pure.elsevier.com/en/publications/mechanism-of-transport-of-ift-particles-in-c-elegans-cilia-by-the
RAE-1, a Novel PHR Binding Protein, Is Required for Axon Termination and Synapse Formation in Caenorhabditis elegans | Journal...RAE-1, a Novel PHR Binding Protein, Is Required for Axon Termination and Synapse Formation in Caenorhabditis elegans | Journal...
The Caenorhabditis elegans Regulator of Presynaptic Morphology (RPM)-1 is a member of a conserved family of proteins called Pam/Highwire/RPM-1 (PHR) proteins. PHR proteins are key regulators of neuronal development. In C. elegans, RPM-1 (regulator of presynaptic morphology-1) regulates axon termination and guidance in the mechanosensory neurons, and regulates synapse formation in the mechanosensory and motor neurons (Po et al., 2010). In adult C. elegans, RPM-1 also plays a role in axon regeneration in motor neurons (Hammarlund et al., 2009). Importantly, Drosophila Highwire, zebrafish Esrom/Phr1, and murine Phr1 also regulate synapse formation and axon extension highlighting the evolutionarily conserved function of the PHR proteins (Po et al., 2010).. Previous studies showed that RPM-1 functions, in part, as an E3 ubiquitin ligase by binding to the F box SyNaptic protein (FSN)-1 and negatively regulating a MAPK pathway that includes: the Dual Leucine zipper-bearing Kinase (DLK)-1, MAPK Kinase ...
http://www.jneurosci.org/content/32/8/2628.long
Vulval development (Caenorhabditis elegans) - WikiPathwaysVulval development (Caenorhabditis elegans) - WikiPathways
The C. elegans hermaphrodite vulva develops during postembryonic (larval) development from ventral epidermal precursors, and connects the developing uterus to the external environment. In the adult, the vulva is necessary for egg-laying (see Egg-laying) and for copulation with males (see Male mating behavior). Vulval development has attracted general interest for three main reasons. First, it serves as a paradigm for organogenesis. In particular, vulva development represents a well-understood case in which invariant development arises from multiple cell-cell interactions. It is also a striking example of tissue remodeling: the formation of a hole at a precise location in an organism. Second, it has been important for the genetic analyses of signaling and signal transduction by epidermal growth factor (EGF)-receptor LET-23 and RAS LET-60; (see RTKRas/MAP kinase signaling), LIN-12 (see LIN-12/Notch signaling in C. elegans), and WNT (see Wnt signaling), as well as the functions of the SynMuv and ...
https://www.wikipathways.org/index.php?title=Pathway:WP1453&direction=prev&oldid=48996
Vulval development (Caenorhabditis elegans) - WikiPathwaysVulval development (Caenorhabditis elegans) - WikiPathways
The C. elegans hermaphrodite vulva develops during postembryonic (larval) development from ventral epidermal precursors, and connects the developing uterus to the external environment. In the adult, the vulva is necessary for egg-laying (see Egg-laying) and for copulation with males (see Male mating behavior). Vulval development has attracted general interest for three main reasons. First, it serves as a paradigm for organogenesis. In particular, vulva development represents a well-understood case in which invariant development arises from multiple cell-cell interactions. It is also a striking example of tissue remodeling: the formation of a hole at a precise location in an organism. Second, it has been important for the genetic analyses of signaling and signal transduction by epidermal growth factor (EGF)-receptor LET-23 and RAS LET-60; (see RTKRas/MAP kinase signaling), LIN-12 (see LIN-12/Notch signaling in C. elegans), and WNT (see Wnt signaling), as well as the functions of the SynMuv and ...
https://www.wikipathways.org/index.php?title=Pathway:WP1453&direction=next&oldid=48997
Regulation of Tcl transposable elements in Caenorhabditis elegans.<...Regulation of Tcl transposable elements in Caenorhabditis elegans.<...
TY - JOUR. T1 - Regulation of Tcl transposable elements in Caenorhabditis elegans.. AU - Emmons, S. W.. AU - Ruan, K. S.. AU - Levitt, A.. AU - Yesner, L.. PY - 1985. Y1 - 1985. N2 - C. elegans strains contain variable numbers of a 1.6-kb transposable genetic element. Activity of this element, which is denoted Tcl, shows regulation at at least two levels. At one level, excision of Tcl elements occurs in somatic cells at a frequency several orders of magnitude higher than in germ cells. Evidence is presented suggesting that this results from regulation at the level of trans-acting functions that are required for excision or that repress excision. At the second level, germ line transposition of Tcl occurs at greater frequency in some strains than in others. The hypothesis is proposed that this is because Tcl is one component of a two-element system, the second element of which differs between strains. Evidence for a second putative transposable element family in C. elegans is presented. This ...
https://einstein.pure.elsevier.com/en/publications/regulation-of-tcl-transposable-elements-in-caenorhabditis-elegans-2
Two Leucobacter strains exert complementary virulence on Caenorhabditis including death by worm-star formation. - Oxford...
The nematode Caenorhabditis elegans has been much studied as a host for microbial infection. Some pathogens can infect its intestine, while others attack via its external surface. Cultures of Caenorhabditis isolated from natural environments have yielded new nematode pathogens, such as microsporidia and viruses. We report here a novel mechanism for bacterial attack on worms, discovered during investigation of a diseased and coinfected natural isolate of Caenorhabditis from Cape Verde. Two related coryneform pathogens (genus Leucobacter) were obtained from this isolate, which had complementary effects on C. elegans and related nematodes. One pathogen, Verde1, was able to cause swimming worms to stick together irreversibly by their tails, leading to the rapid formation of aggregated worm-stars. Adult worms trapped in these aggregates were immobilized and subsequently died, with concomitant growth of bacteria. Trapped larval worms were sometimes able to escape from worm-stars by undergoing autotomy,
https://www.neuroscience.ox.ac.uk/publications/438711
Cytoplasmic flow and the establishment of polarity in C. elegans 1-cell embryos. - Semantic ScholarCytoplasmic flow and the establishment of polarity in C. elegans 1-cell embryos. - Semantic Scholar
Early Caenorhabditis elegans embryos provide an excellent model for the study of developmental processes. Development can be studied by direct observation under the light microscope and can be perturbed using laser manipulations, drug inhibitor treatments, and genetic mutants. The first division of the C. elegans embryo is asymmetric, generating two daughter cells unequal in size and developmental fate. These distinct fates are generated by the partitioning of cytoplasmic determinants during the first mitotic cell cycle. Partitioning of these determinants is thought to be driven by cytoplasmic flow. Recent studies in C. elegans in the past year have identified a number of components necessary for this flow, giving us a clearer picture of the molecular mechanisms underlying developmental asymmetry.
https://www.semanticscholar.org/paper/Cytoplasmic-flow-and-the-establishment-of-polarity-Golden/1d26ef70096bd86ec87705c7fdafc55f299881a8
Functional and phylogenetic analysis of the ubiquitylation system in Caenorhabditis elegans : ubiquitin-conjugating enzymes,...Functional and phylogenetic analysis of the ubiquitylation system in Caenorhabditis elegans : ubiquitin-conjugating enzymes,...
The eukaryotic ubiquitin-conjugation system sets the turnover rate of many proteins and includes activating enzymes (E1s), conjugating enzymes (UBCs/E2s), and ubiquitin-protein ligases (E3s), which are responsible for activation, covalent attachment and substrate recognition, respectively. There are also ubiquitin-like proteins with distinct functions, which require their own E1s and E2s for attachment. We describe the results of RNA interference (RNAi) experiments on the E1s, UBC/E2s and ubiquitin-like proteins in Caenorhabditis elegans. We also present a phylogenetic analysis of UBCs. The C. elegans genome encodes 20 UBCs and three ubiquitin E2 variant proteins. RNAi shows that only four UBCs are essential for embryogenesis: LET-70 (UBC-2), a functional homolog of yeast Ubc4/5p, UBC-9, an ortholog of yeast Ubc9p, which transfers the ubiquitin-like modifier SUMO, UBC-12, an ortholog of yeast Ubc12p, which transfers the ubiquitin-like modifier Rub1/Nedd8, and UBC-14, an ortholog of Drosophila Courtless.
https://genomebiology.biomedcentral.com/articles/10.1186/gb-2001-3-1-research0002
Family of FLP peptides in Caenorhabditis elegans and related nematodes by Chris Li and Kyuhyung KimFamily of FLP peptides in Caenorhabditis elegans and related nematodes by Chris Li and Kyuhyung Kim
Neuropeptides regulate all aspects of behavior in multicellular organisms. Because of their ability to act at long distances, neuropeptides can exert their effects beyond the conventional synaptic connections, thereby adding an intricate layer of complexity to the activity of neural networks. In the nematode Caenorhabditis elegans, a large number of neuropeptide genes that are expressed throughout the nervous system have been identified.The actions of these peptides supplement the synaptic connections of the 302 neurons, allowing for fine tuning of neural networks and increasing the ways in which behaviors can be regulated. In this review, we focus on a large family of genes encoding FMRFamide-related peptides (FaRPs). These genes, the flp genes, have been used as a starting point to identifying flp genes throughout Nematoda. Nematodes have the largest family of FaRPs described thus far. The challenges in the future are the elucidation of their functions and the identification of the receptors and
https://academicworks.cuny.edu/cc_pubs/395/
Frontiers | Genome-Wide Analyses of Metal Responsive Genes in Caenorhabditis elegans | GeneticsFrontiers | Genome-Wide Analyses of Metal Responsive Genes in Caenorhabditis elegans | Genetics
Metals are major contaminants that influence human health. Many metals have physiologic roles, but excessive levels can be harmful. Advances in technology have made toxicogenomic analyses possible to characterize the effects of metal exposure on the entire genome. Much of what is known about cellular responses to metals has come from mammalian systems; however the use of non-mammalian species is gaining wider attention. Caenorhabditis elegans (C. elegans) is a small round worm whose genome has been fully sequenced and its development from egg to adult is well characterized. It is an attractive model for high throughput screens due to its short lifespan, ease of genetic mutability, low cost and high homology with humans. Research performed in C. elegans has led to insights in apoptosis, gene expression and neurodegeneration, all of which can be altered by metal exposure. Additionally, by using worms one can potentially study how the mechanisms that underline differential responses to metals in nematodes
https://www.frontiersin.org/articles/10.3389/fgene.2012.00052/full
Navigational choice between reversal and curve during acidic pH avoidance behavior in Caenorhabditis elegans | BMC Neuroscience...Navigational choice between reversal and curve during acidic pH avoidance behavior in Caenorhabditis elegans | BMC Neuroscience...
Under experimental conditions, virtually all behaviors of Caenorhabditis elegans are achieved by combinations of simple locomotion, including forward, reversal movement, turning by deep body bending, and gradual shallow turning. To study how worms regulate these locomotion in response to sensory information, acidic pH avoidance behavior was analyzed by using worm tracking system. In the acidic pH avoidance, we characterized two types of behavioral maneuvers that have similar behavioral sequences in chemotaxis and thermotaxis. A stereotypic reversal-turn-forward sequence of reversal avoidance caused an abrupt random reorientation, and a shallow gradual turn in curve avoidance caused non-random reorientation in a less acidic direction to avoid the acidic pH. Our results suggest that these two maneuvers were each triggered by a distinct threshold pH. A simulation study using the two-distinct-threshold model reproduced the avoidance behavior of the real worm, supporting the presence of the threshold.
https://bmcneurosci.biomedcentral.com/articles/10.1186/s12868-015-0220-0
Cloning by insertional mutagenesis of a cDNA encoding Caenorhabditis elegans kinesin heavy chain | PNASCloning by insertional mutagenesis of a cDNA encoding Caenorhabditis elegans kinesin heavy chain | PNAS
An additional genetic locus in Caenorhabditis elegans, unc-116, was identified in a screen for mutations resulting in defective locomotion. unc-116 was cloned by use of a transposon insertion mutant and the physical and genetic map of the genome. The cDNA sequence predicts an 815-amino acid protein. Based upon sequence comparison and secondary structure predictions, unc-116 encodes all three domains of the kinesin heavy chain: the motor, stalk, and tail. While the motor and tail domains have a high degree of identity to the equivalent domains of cloned kinesin heavy chains, the rodII domain of the stalk is significantly shorter than those previously reported and is not predicted to form a coiled-coil alpha-helix. Analysis of mutational defects in two C. elegans genes encoding anterograde motor molecules, unc-116 and unc-104, should provide insight into the in vivo functions of these members of the kinesin heavy chain superfamily.. ...
https://www.pnas.org/content/90/19/9181?ijkey=68f23e79acd58a48d14f6935392cf77e21206eba&keytype2=tf_ipsecsha
lin-12 - Protein lin-12 precursor - Caenorhabditis elegans - lin-12 gene & proteinlin-12 - Protein lin-12 precursor - Caenorhabditis elegans - lin-12 gene & protein
Involved in several cell fate decisions that require cell-cell interactions. It is possible that lin-12 encodes a membrane-bound receptor for a signal that enables expression of the ventral uterine precursor cell fate. Activity in cell fate decisions and tumorigenesis is negatively regulated by sel-10.
http://www.uniprot.org/uniprot/P14585
Regulation of Hox gene expression and posterior development by the Xenopus caudal homologue Xcad3 | The EMBO Journal
The prototype of the Cdx family of homeodomain transcription factors is the Drosophila caudal protein. The initial maternal expression of caudal mRNA is ubiquitous and a posterior to anterior gradient of the protein develops during the syncytial blastoderm stage and persists until the onset of cellularization. Zygotic expression, which commences in the cellular blastoderm stage, is also localized to the posterior in a region which gives rise to terminal abdominal structures and the hindgut. During later embryonic development, expression of caudal is found in the midgut, hindgut and Malpigian tubules (MacDonald and Struhl, 1986; Mlodzik and Gehring, 1987).. Caudal homologues have been identified in a wide range of animal groups. A caudal‐related gene with a similar posterior expression pattern has been cloned from the short or intermediate germ band insect Bombyx mori and homologues are present in other invertebrates, including the nematode worm Caenorhabditis elegans and the annelid worm ...
http://emboj.embopress.org/content/17/12/3413
Mitochondrial stress extends lifespan in C. elegans through neuronal hormesis. - Semantic ScholarMitochondrial stress extends lifespan in C. elegans through neuronal hormesis. - Semantic Scholar
Progressive neuronal deterioration accompanied by sensory functions decline is typically observed during aging. On the other hand, structural or functional alterations of specific sensory neurons extend lifespan in the nematode Caenorhabditis elegans. Hormesis is a phenomenon by which the body benefits from moderate stress of various kinds which at high doses are harmful. Several studies indicate that different stressors can hormetically extend lifespan in C. elegans and suggest that hormetic effects could be exploited as a strategy to slow down aging and the development of age-associated (neuronal) diseases in humans. Mitochondria play a central role in the aging process and hormetic-like bimodal dose-response effects on C. elegans lifespan have been observed following different levels of mitochondrial stress. Here we tested the hypothesis that mitochondrial stress may hormetically extend C. elegans lifespan through subtle neuronal alterations. In support of our hypothesis we find that life-lengthening
https://www.semanticscholar.org/paper/Mitochondrial-stress-extends-lifespan-in-C-elegans-Maglioni-Schiavi/2e0d4102f4571f7fff8933d23c53c4f851939b4f
Neural control of Caenorhabditis elegans forward locomotion: the role of sensory feedback - White Rose Research OnlineNeural control of Caenorhabditis elegans forward locomotion: the role of sensory feedback - White Rose Research Online
This paper presents a simple yet biologicallygrounded model for the neural control of Caenorhabditis elegans forward locomotion. We identify a minimal circuit within the C. elegans ventral cord that is likely to be sufficient to generate and sustain forward locomotion in vivo. This limited subcircuit appears to contain no obvious central pattern generated control. For that subcircuit, we present a model that relies on a chain of oscillators along the body which are driven by local and proximate mechano-sensory input. Computer simulations were used to study the model under a variety of conditions and to test whether it is behaviourally plausible. Within our model, we find that a minimal circuit of AVB interneurons and B-class motoneurons is sufficient to generate and sustain fictive forward locomotion patterns that are robust to significant environmental perturbations. The model predicts speed and amplitude modulation by the AVB command interneurons. An extended model including D-class ...
http://eprints.whiterose.ac.uk/7948/
Differential Expression of Glutamate Receptor Subunits in the Nervous System of Caenorhabditis elegans and Their Regulation by...Differential Expression of Glutamate Receptor Subunits in the Nervous System of Caenorhabditis elegans and Their Regulation by...
Strains. Nematodes were grown at 20°C under standard conditions that included uncrowded conditions and the presence of ample food (the Escherichia coli strain OP50). Wild-type nematodes were C. elegans strain N2. Mutant strains were obtained from the Caenorhabditis Genetic Center.. cDNA clones and expression constructs. Using degenerate oligonucleotide primers designed to amplify conserved regions of ionotropic glutamate receptors, we amplified DNA fragments from first-strand mixed-stage C. elegans cDNA that encoded portions of glr-3 and glr-5. These partial gene products were used to screen cDNA libraries at high stringency. After screening ∼106 clones, we obtained several partial cDNAs for each gene, indicating that mRNA encoding these glutamate receptor subunits is present at relatively low levels. Hence, we were unable to isolate full-length cDNAs using this approach. We also searched the published C. elegans genome for genes related in sequence to glr-1, glr-3, andglr-5 and identified ...
http://www.jneurosci.org/content/21/5/1510.long
OSA | Multiphoton laser scanning microscopy for four-dimensional analysis of Caenorhabditis elegans embryonic development.OSA | Multiphoton laser scanning microscopy for four-dimensional analysis of Caenorhabditis elegans embryonic development.
Multiphoton laser scanning microscopy (MPLSM) enables the production of long timelapse recordings from live fluorescent specimens. 1047- and 900-nm excitation were used to image both a vital fluorescent membrane probe, FM 4-64, and a modified green fluorescent protein (GFP) in live Caenorhabditis elegans embryos. Automated four-dimensional (4D) data collection yielded individual recordings comprising thousands of images, each allowing analysis of all of the cell divisions, contacts, migrations, and fusions that occur during a span of several hours of embryogenesis.. ©1998 Optical Society of America. Full Article , PDF Article ...
https://www.osapublishing.org/oe/abstract.cfm?URI=oe-3-9-325
MicroRNA targeting in mus musculus and Caenorhabditis elegansMicroRNA targeting in mus musculus and Caenorhabditis elegans
MicroRNAs (miRNAs) are small, approximately 22 nucleotide RNAs that regulate gene expression post-transcriptionally by base-pairing to complementary sites in the target mRNA. The first miRNA, lin-4, was discovered in 1993 in Caenorhabditis elegans; since then hundreds of miRNAs have been identified in C. elegans, Drosophila melanogaster, plants, mouse, and humans, where they approach a number equivalent to 1-2% of the protein-coding genes. With the exception of plants, miRNAs most commonly regulate targets by imperfectly pairing to 3 untranslated regions (UTRs), leading to translational repression or mRNA destabilization. The microRNA miR-196 is encoded at three paralogous locations in the HoxA, B, and C clusters in mammals and has conserved complementarity to the 3UTRs of Hoxb8, Hoxc8, and Hoxa7; in particular, miR-1 96 has complete complementarity to Hoxb8 with the exception of a single G:U wobble. In 2004, Yekta et al., were able to detect RNA fragments diagnostic of miR-1 96-directed ...
https://dspace.mit.edu/handle/1721.1/68432
Molecular and genetic characterization of GON-2, a TRPM cation channel required for gonadogenesis in Caenorhabditis elegans. ::...Molecular and genetic characterization of GON-2, a TRPM cation channel required for gonadogenesis in Caenorhabditis elegans. ::...
0, Molecular and genetic characterization of GON-2, a TRPM cation channel required for gonadogenesis in Caenorhabditis elegans A Thesis Submitted to the Faculty in partial fulfillment of the requirements for the degree of o Doctor of Philosophy in Biological Sciences by Rachel West DARTMOUTH COLLEGE Hanover, New Hampshire January, 2004 Examining9ommittee: Eric }. IAInbig (Chair) c:, Victor R. A)fibros Eleanor M. Maine Carol L. Folt Dean of Graduate Studies ...
http://libarchive.dartmouth.edu/cdm/compoundobject/collection/dcdis/id/809/rec/1
Caenorhabditis elegans as an environmental monitor using DNA microarray analysis<...
TY - JOUR. T1 - Caenorhabditis elegans as an environmental monitor using DNA microarray analysis. AU - Custodia, N.. AU - Won, S. J.. AU - Novillo, A.. AU - Wieland, M.. AU - Li, C.. AU - Callard, I. P.. N1 - Copyright: Copyright 2020 Elsevier B.V., All rights reserved.. PY - 2001. Y1 - 2001. N2 - In order to assist in the identification of possible endocrine disrupting chemicals (EDC) in groundwater, we are developing Caenorhabolitis elegans as a high throughput bioassay system in which responses to EDC may be detected by gene expression using DNA microarray analysis. As a first step we examined gene expression patterns and vitellogenin responses of this organism to vertebrate steroids, in liquid culture. Western blotting showed the expected number and size of vitellogenin translation products after estrogen exposure. At 10-9 M, vitellogenin decreased, but at 10-7 and 10-5, vitellogenin was increased. Testosterone (10-5 M) increased the synthesis of vitellogenin, but progesterone-treated ...
https://mayoclinic.pure.elsevier.com/en/publications/caenorhabditis-elegans-as-an-environmental-monitor-using-dna-micr
PRIME PubMed | Caenorhabditis elegans EAK-3 inhibits dauer arrest via nonautonomous regulation of nuclear DAF-16/FoxO activityPRIME PubMed | Caenorhabditis elegans EAK-3 inhibits dauer arrest via nonautonomous regulation of nuclear DAF-16/FoxO activity
PubMed journal article: Caenorhabditis elegans EAK-3 inhibits dauer arrest via nonautonomous regulation of nuclear DAF-16/FoxO activity. Download Prime PubMed App to iPhone, iPad, or Android
https://www.unboundmedicine.com/medline/citation/18241854/Caenorhabditis_elegans_EAK_3_inhibits_dauer_arrest_via_nonautonomous_regulation_of_nuclear_DAF_16/FoxO_activity_
Hubers Killi-Data - Aphyosemion elegans, information snapshot (Cyprinodontiformes, Killifishes)Hubers Killi-Data - Aphyosemion elegans, information snapshot (Cyprinodontiformes, Killifishes)
Current Name: Aphyosemion elegans. Describer(s), Year: (Boulenger, 1899). IDENTITY: elegans A.. Family-group Names: Nothobranchiidae Garman, 1895 , Nothobranchiinae Garman, 1895 , Nothobranchiini Garman, 1895 , Aphyosemina Huber, 2000 (#Aphyosemiina #Aphyosemionina) Genus: Aphyosemion Myers, 1924. Subgenus: Aphyosemion Myers, 1924. Abbreviated genus: A.. Abbreviated subgenus: (A.). Species: elegans. Index name: elegans: Aphyosemion elegans. Full name: Aphyosemion (Aphyosemion) elegans. TYPOLOGY: elegans A.. Original name: Haplochilus elegans. Describer(s): Boulenger. Year of description: 1899. Original description: Boulenger, G.A. 1899. Poissons nouveaux du Congo. Cinquième Partie. Cyprins, Silures, Cyprinodontes, Acanthopterygiens. Ann. Mus. Congo Belge, Tervuren, Zool. Ser., 1 (5): 113, pl. 47 (fig. 2).. Gender/Accordance: [Subst.]. SYSTEMATICS: elegans A.. Current status: valid sp.. Status evaluation (current): discussed {no red bars on male sides are mentioned in the description, only -red- ...
http://killi-data.org/zz-elegansHaplo.php
Sel-12Sel-12
The Caenorhabditis elegans sel-12 gene encodes a multi-pass transmembrane domain protein that is similar to human presenilin. ... a Caenorhabditis elegans S182 Alzheimer's disease gene". Nature. 377 (6547): 351-354. Bibcode:1995Natur.377..351L. doi:10.1038/ ... sel-12 also plays a role in thermotaxis (the nematode worm prefers a certain temperature and moves accordingly). C. elegans ... Stefan Eimer (2003). "Analysis and suppression of mutant sel-12 in C. elegans". Ph.D. Thesis, Ludwig-Maximilians Universität, ...
https://en.wikipedia.org/wiki/Sel-12
Crustacean neurohormone familyCrustacean neurohormone family
Caenorhabditis elegans uncharacterised protein ZC168.2. These neurohormones are peptides of 70 to 80 amino acid residues which ... In molecular biology, the crustacean neurohormone family of proteins is a family of neuropeptides expressed by arthropods. The ...
https://en.wikipedia.org/wiki/Crustacean_neurohormone_family
FERM domainFERM domain
Protein-tyrosine phosphatases PTPN14 and PTP-D1, PTP-RL10 and PTP2E. Caenorhabditis elegans protein phosphatase ptp-1. Chishti ... Ezrin, moesin, and radixin are highly related proteins (ERM protein family), but the other proteins in which the FERM domain is ... In molecular biology, the FERM domain (F for 4.1 protein, E for ezrin, R for radixin and M for moesin) is a widespread protein ... Non-receptor tyrosine-protein kinase TYK2. Protein-tyrosine phosphatases PTPN3 and PTPN4, enzymes that appear to act at ...
https://en.wikipedia.org/wiki/FERM_domain
Immunoglobulin C2-set domainImmunoglobulin C2-set domain
Chothia C, Teichmann SA (2000). "Immunoglobulin superfamily proteins in Caenorhabditis elegans". J. Mol. Biol. 296 (5): 1367-83 ... "Protein-Protein Recognition: Juxtaposition of Domain and Interface Cores in Immunoglobulins and Other Sandwich-like Proteins". ... This article incorporates text from the public domain Pfam and InterPro: IPR008424 (Protein pages needing a picture, Protein ... Immunoglobulin-like domains that are related in both sequence and structure can be found in several diverse protein families. ...
https://en.wikipedia.org/wiki/Immunoglobulin_C2-set_domain
Ced-12Ced-12
Protein pages needing a picture, Caenorhabditis elegans genes). ... containing adaptor protein found in Caenorhabditis elegans and ... Researchers also noted direct protein-protein interactions between CED-12 and CED-10 (C. elegans homolog for Rac1), a Rac- ... The first is by CED-6, which is an adaptor protein that is responsible for coordinating protein-protein interactions between ... It is found on chromosome 2 on the L-arm in Drosophila, and on chromosome I in C. elegans. The protein structure of CED-12 is ...
https://en.wikipedia.org/wiki/Ced-12
Ced-9Ced-9
"ced-9 - Apoptosis regulator ced-9 - Caenorhabditis elegans - ced-9 gene & protein". www.uniprot.org. Retrieved 2017-11-16. ... Protein pages needing a picture, Caenorhabditis elegans genes). ... The CED-9 protein belongs to the Bcl-2-like protein family. ... The protein is homologous to the human apoptotic regulator Bcl-2 as well as all other proteins in the Bcl-2 protein family. CED ... elegans. The protein consists of 280 amino acids and has a molecular weight of 31824.42 Da. The structure of this protein has ...
https://en.wikipedia.org/wiki/Ced-9
Ced-5Ced-5
Protein pages needing a picture, Caenorhabditis elegans genes). ... elegans phagocytosis and cell-migration protein CED-5 is ... CED-5 is an ortholog of the mammalian protein Dock180，which present in the nematode worm C. elegans., the Drosophila ...
https://en.wikipedia.org/wiki/Ced-5
Ced-3Ced-3
... is one of the major protein components of the programmed cell death (PCD) pathway for Caenorhabditis elegans. There are ... "ced-3 Cell death protein 3 [ Caenorhabditis elegans ]". The National Center for Biotechnology Information. 2017-10-12. Shaham S ... elegans but for mammals as well. One of the main roles of the ced-3 protein in C. elegans is to help the development and growth ... "Direct physical interaction between the Caenorhabditis elegans 'death proteins' CED-3 and CED-4". FEBS Letters. 406 (1-2): 189- ...
https://en.wikipedia.org/wiki/Ced-3
Epistasis and functional genomicsEpistasis and functional genomics
"Lin-26 Transcription factor lin-26 [Caenorhabditis elegans] - Gene - NCBI". "Lin-36 Protein lin-36 [Caenorhabditis elegans] - ... Data from protein-protein interaction studies can also provide a useful basis for selecting gene groups for E-MAP data. We ... and their data demonstrate that an E-MAP approach identifies protein-protein interactions with a specificity equal to that of ... 2007). "Functional dissection of protein complexes involved in yeast chromosome biology using a genetic interaction map". ...
https://en.wikipedia.org/wiki/Epistasis_and_functional_genomics
Daf-12Daf-12
Protein pages needing a picture, Caenorhabditis elegans genes, Nuclear receptors). ... in the worm Caenorhabditis elegans, with the NRNC Symbol NR1J1 as the homolog of nuclear hormone receptor HR96 (Hr96) in ... "The Role of Dafachronic Acid Signaling in Development and Longevity in Caenorhabditis elegans: Digging Deeper Using Cutting- ... Kenyon C, Chang J, Gensch E, Rudner A, Tabtiang R (1993). "A C. elegans mutant that lives twice as long as wild type". Nature. ...
https://en.wikipedia.org/wiki/Daf-12
WormBookWormBook
Bioinformatics, Biology books, Cell biology, Caenorhabditis elegans, Proteins, Animal developmental biology). ... peer-reviewed chapters covering topics related to the biology of the nematode worm Caenorhabditis elegans (C. elegans). ... Corsi, Ann K. (2015). "A Transparent window into biology: A primer on Caenorhabditis elegans". WormBook: 1-31. doi:10.1895/ ... The C. elegans Sequencing Consortium (1998). "Genome sequence of the nematode C. elegans: A platform for investigating biology ...
https://en.wikipedia.org/wiki/WormBook
Richard I. MorimotoRichard I. Morimoto
Prahlad, V; Morimoto, RI (2011). "Neuronal circuitry regulates the response of Caenorhabditis elegans to misfolded proteins". ... "Neuronal Signaling Modulates Protein Homeostasis in Caenorhabditis elegans Postsynaptic Muscle Cells". Genes & Development. 21 ... In vivo Properties of the Disaggregase Function of J-domain Proteins and Hsc70 in C. elegans Stress and Aging. Aging Cell 16: ... Prahlad, V.; Cornelius, T.; Morimoto, R.I. (2008). "Regulation of the Cellular Heat Shock Response in Caenorhabditis elegans by ...
https://en.wikipedia.org/wiki/Richard_I._Morimoto
BED zinc fingerBED zinc finger
Caenorhabditis elegans Dpy-20 protein, a predicted cuticular gene transcriptional regulator; Drosophila BEAF (boundary element- ... In molecular biology the BED-type zinc finger domain is a protein domain which was named after the Drosophila proteins BEAF and ... Some proteins known to contain a BED domain include animal, plant and fungi AC1 and Hobo-like transposases; ... This article incorporates text from the public domain Pfam and InterPro: IPR003656 (Protein domains). ...
https://en.wikipedia.org/wiki/BED_zinc_finger
IMD domainIMD domain
Caenorhabditis elegans M04F3.5 protein. The vertebrate IRSp53/MIM family is divided into two major groups: the IRSp53 subfamily ... Vertebrate brain-specific angiogenesis inhibitor 1-associated protein 2-like proteins 1 and 2 (BAI1-associated protein 2-like ... Vertebrate ABBA-1 (MTSS1L), a MIM-related protein. Vertebrate brain-specific angiogenesis inhibitor 1-associated protein 2 ( ... a multifunctional adaptor protein that links Rac1 with a Wiskott-Aldrich syndrome family verprolin-homologous protein 2 (WAVE2/ ...
https://en.wikipedia.org/wiki/IMD_domain
RoGFPRoGFP
"Regulated spatial organization and sensitivity of cytosolic protein oxidation in Caenorhabditis elegans". Nature Communications ... The reduction-oxidation sensitive green fluorescent protein (roGFP) is a green fluorescent protein engineered to be sensitive ... elegans. In addition, roGFPs are used to investigate the topology of ER proteins, or to analyze the ROS production capacity of ... The resulting engineered protein could exist in two different oxidation states (reduced dithiol or oxidized disulfide), each ...
https://en.wikipedia.org/wiki/RoGFP
Vasa geneVasa gene
"Genetic analysis of the Caenorhabditis elegans GLH family of P-granule proteins". Genetics. 178 (4): 1973-87. doi:10.1534/ ... The protein product in humans has 724 amino acids, a molecular mass of 79 kDa and 8 conserved domains in all DEAD-box proteins ... Many other proteins in Drosophila are also localized to the poles. For example, oskar protein was found to localize to pole ... Vasa is an RNA binding protein with an ATP-dependent RNA helicase that is a member of the DEAD box family of proteins. The vasa ...
https://en.wikipedia.org/wiki/Vasa_gene
Galactose binding lectin domainGalactose binding lectin domain
The hypothetical B0457.1, F32A7.3A and F32A7.3B proteins from Caenorhabditis elegans. Ozeki Y, Matsui T, Suzuki M, Titani K ( ... In molecular biology, the galactose binding lectin domain is a protein domain. It is found in many proteins including the ... homologous to the SUEL protein has been identified in the following proteins: Plant beta-galactosidases EC 3.2.1.23 (lactases ... This protein is composed of three tandem repeat domains homologous to the SUEL lectin domain. All cysteine positions of each ...
https://en.wikipedia.org/wiki/Galactose_binding_lectin_domain
LITE-1LITE-1
v t e (All stub articles, Protein stubs, Caenorhabditis elegans genes, Photoreceptor cells). ... LITE-1 is a novel photoreceptor found in Caenorhabditis elegans. It exhibits blue light photoreceptor activity. Is involved in ... Many organisms have photosensitive proteins, yet only two types of photoreceptors, opsins and cryptochromes, have been ... elegans Taste Receptor Homolog LITE-1 Is a Photoreceptor". Cell. 167 (5): 1252-1263.e10. doi:10.1016/j.cell.2016.10.053. ISSN ...
https://en.wikipedia.org/wiki/LITE-1
UNC93B1UNC93B1
This gene encodes a protein with similarity to the Caenorhabditis elegans unc93 protein. The Unc93 protein is involved in the ... Unc-93 homolog B1 (C. elegans), also known as UNC93B1, is a protein which in humans is encoded by the UNC93B1 gene. ... This protein is an intrinsic membrane protein that spans the membrane twelve times. It is found in the endoplasmic reticulum ... Unc93B1 protein appears to be involved in the innate immune response. Defects in the protein predispose to hypersensitity to ...
https://en.wikipedia.org/wiki/UNC93B1
RBFOX1RBFOX1
"Homologues of the Caenorhabditis elegans Fox-1 protein are neuronal splicing regulators in mammals". Molecular and Cellular ... or hexaribonucleotide-binding protein 1 (HRNBP1) or RNA binding protein, fox-1 homolog (Rbfox1), is a protein that in humans is ... Rbfox1 has an RNA recognition motif that is highly conserved among RNA-binding proteins. Rbfox1, and the related protein Rbfox2 ... "Entrez Gene: A2BP1 ataxin 2-binding protein 1". Jin, Y. (2003-02-17). "A vertebrate RNA-binding protein Fox-1 regulates tissue- ...
https://en.wikipedia.org/wiki/RBFOX1
NeuNNeuN
... a protein which is a homologue to the protein product of a sex-determining gene in Caenorhabditis elegans, is a neuronal ... Underwood JG, Boutz PL, Dougherty JD, Stoilov P, Black DL (2005). "Homologues of the Caenorhabditis elegans Fox-1 protein are ... Fox-3 is one of a family of mammalian homologues of the Fox-1 protein, originally discovered in the nematode worm C. elegans as ... a protein originally identified from genetic studies of the nematode worm C. elegans. Western blotting shows that mAb A60 binds ...
https://en.wikipedia.org/wiki/NeuN
Andrew DillinAndrew Dillin
His lab studies the loss of protein homeostasis in aging, particularly in Caenorhabditis elegans. His lab specifically looks at ... the manipulation of stress response pathways, such as the heat shock response and the unfolded protein response of the ...
https://en.wikipedia.org/wiki/Andrew_Dillin
FHITFHIT
"Nitrilase and Fhit homologs are encoded as fusion proteins in Drosophila melanogaster and Caenorhabditis elegans". Proc. Natl. ... Bis(5'-adenosyl)-triphosphatase also known as fragile histidine triad protein (FHIT) is an enzyme that in humans is encoded by ...
https://en.wikipedia.org/wiki/FHIT
SbRNASbRNA
"Microarray analysis of ncRNA expression patterns in Caenorhabditis elegans after RNAi against snoRNA associated proteins". BMC ... Li T; He H; Wang Y; Zheng H; Skogerbø G; Chen R (2008). "In vivo analysis of Caenorhabditis elegans noncoding RNA promoter ... sbRNA (stem-bulge RNA) is a family of non-coding RNA first discovered in Caenorhabditis elegans. It was identified during a ... March 2005). "Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans". Nature. 434 (7032): 462-9. doi: ...
https://en.wikipedia.org/wiki/SbRNA
UNC93AUNC93A
Levin JZ, Horvitz HR (April 1992). "The Caenorhabditis elegans unc-93 gene encodes a putative transmembrane protein that ... Unc-93 homolog A (C. elegans) is a protein that in humans is encoded by the UNC93A gene. Unc93A is a major facilitator ... and unc-93 may encode components of a two-pore K+ channel that coordinates muscle contraction in Caenorhabditis elegans". The ... Read also for functional studies in C.elegans. For you who are interested to read more about Unc93A in different species, see: ...
https://en.wikipedia.org/wiki/UNC93A
Cell nucleusCell nucleus
"Stress induced nuclear granules form in response to accumulation of misfolded proteins in Caenorhabditis elegans". Primary. BMC ... The nuclear lamina is composed mostly of lamin proteins. Like all proteins, lamins are synthesized in the cytoplasm and later ... Speckles are dynamic structures, and both their protein and RNA-protein components can cycle continuously between speckles and ... Both structures serve to mediate binding to nuclear transport proteins.: 509-10 Most proteins, ribosomal subunits, and some ...
https://en.wikipedia.org/wiki/Cell_nucleus
GluconeogenesisGluconeogenesis
"Bifunctional glyoxylate cycle protein of Caenorhabditis elegans: a developmentally regulated protein of intestine and muscle". ... Transport of PEP across the mitochondrial membrane is accomplished by dedicated transport proteins; however no such proteins ... it triggers phosphorylation of enzymes and regulatory proteins by Protein Kinase A (a cyclic AMP regulated kinase) resulting in ... In the liver, the FOX protein FOXO6 normally promotes gluconeogenesis in the fasted state, but insulin blocks FOXO6 upon ...
https://en.wikipedia.org/wiki/Gluconeogenesis
ARL13BARL13B
2010). "Joubert syndrome Arl13b functions at ciliary membranes and stabilizes protein transport in Caenorhabditis elegans". J. ... ADP-ribosylation factor-like protein 13B (ARL13B), also known as ADP-ribosylation factor-like protein 2-like 1, is a protein ... This protein is localized in the cilia and plays a role in cilia formation and in maintenance of cilia. Mutations in the ARL13B ... The encoded protein is a small GTPase that contains both N-terminal and C-terminal guanine nucleotide-binding motifs. ...
https://en.wikipedia.org/wiki/ARL13B
Daf-8Daf-8
v t e (Protein pages needing a picture, Caenorhabditis elegans genes, All stub articles, Gene stubs). ... which was originally found in model organism Caenorhabditis elegans. When the TGF-β ligand daf-7 binds to the TGF-β receptors ... Gumienny TL, Savage-Dunn C (July 2013). "TGF-β signaling in C. elegans". WormBook: 1-34. doi:10.1895/wormbook.1.22.2. PMC ... The DAF-8 nematode gene encoding a R-SMAD protein of TGF-beta signaling pathway, ...
https://en.wikipedia.org/wiki/Daf-8
Daf-1Daf-1
Articles with short description, Short description matches Wikidata, Protein pages needing a picture, Caenorhabditis elegans ... the SMAD proteins in C. elegans. Georgi LL, Albert PS, Riddle DL (May 1990). "daf-1, a C. elegans gene controlling dauer larva ... of TGFbeta signaling is conferred by distinct type I receptors and their associated SMAD proteins in Caenorhabditis elegans". ... Morita K, Shimizu M, Shibuya H, Ueno N (May 2001). "A DAF-1-binding protein BRA-1 is a negative regulator of DAF-7 TGF-beta ...
https://en.wikipedia.org/wiki/Daf-1
Uridine monophosphate synthaseUridine monophosphate synthase
Deficiency of the enzyme can be studied in the model organism Caenorhabditis elegans. The rad-6 strain has a premature stop ... "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957-68. doi:10.1016/j.cell ... Merry A (2007). Characterisation and Identification of a Radiation Sensitive Mutant in Caenorhabditis elegans (Ph.D.). ... It is believed that the two separate catalytic sites fused into a single protein to stabilize its monomeric form. The covalent ...
https://en.wikipedia.org/wiki/Uridine_monophosphate_synthase
WIPI2WIPI2
2000). "Identification of Novel Human Genes Evolutionarily Conserved in Caenorhabditis elegans by Comparative Proteomics". ... WD repeat domain phosphoinositide-interacting protein 2 is a protein that in humans is encoded by the WIPI2 gene. WD40 repeat ... 2001). "Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs ... Members of the WIPI subfamily of WD40 repeat proteins, such as WIPI2, have a 7-bladed propeller structure and contain a ...
https://en.wikipedia.org/wiki/WIPI2
Endless Forms Most Beautiful (book)Endless Forms Most Beautiful (book)
... the nematode Caenorhabditis elegans)?" asks Erwin. He answered his own question about the "astonishing morphological diversity ... of their proteins, a figure that rises to 95% for humans and mice. Thus we can't exclude protein-sequence evolution as an ... Most of the changes are in genetic control, not in proteins. 11. Endless Forms Most Beautiful Carroll concludes by revisiting ... which do not code for structural proteins (such as enzymes), control embryonic development. In turn, these regulatory genes ...
https://en.wikipedia.org/wiki/Endless_Forms_Most_Beautiful_(book)
SenescenceSenescence
... ranging from the simple budding yeast Saccharomyces cerevisiae to worms such as Caenorhabditis elegans and fruit flies ( ... Free radicals can damage proteins, lipids or DNA. Glycation mainly damages proteins. Damaged proteins and lipids accumulate in ... Chemical damage to structural proteins can lead to loss of function; for example, damage to collagen of blood vessel walls can ... These adducts can further rearrange to form reactive species, which can then cross-link the structural proteins or DNA to ...
https://en.wikipedia.org/wiki/Senescence
Mitochondrial ribosomal protein L4Mitochondrial ribosomal protein L4
2000). "Identification of Novel Human Genes Evolutionarily Conserved in Caenorhabditis elegans by Comparative Proteomics". ... 39S ribosomal protein L4, mitochondrial is a protein that in humans is encoded by the MRPL4 gene. Mammalian mitochondrial ... 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. Bibcode: ... Systematic analysis of protein components of the large ribosomal subunit from mammalian mitochondria". J. Biol. Chem. 276 (24 ...
https://en.wikipedia.org/wiki/Mitochondrial_ribosomal_protein_L4
Eileen SouthgateEileen Southgate
... is a British biologist who mapped the complete nervous system of the roundworm Caenorhabditis elegans (C. ... Southgate initially worked for Max Perutz and John Kendrew studying hemoglobin, the protein responsible for carrying oxygen ... 1986) 'The structure of the nervous system of the nematode Caenorhabditis elegans'". Philosophical Transactions of the Royal ... Ankeny, Rachel A. (June 2001). "The natural history of Caenorhabditis elegans research". Nature Reviews Genetics. 2 (6): 474- ...
https://en.wikipedia.org/wiki/Eileen_Southgate
Short interspersed nuclear elementShort interspersed nuclear element
"An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans". Science. 294 (5543): 858-62. Bibcode: ... Furthermore, SINEs frequently contain motifs for YY1 polycomb proteins. YY1 is a zinc-finger protein that acts as a ... Thereafter, one of the strands is incorporated into a multi-protein RNA-induced silencing complex (RISC). Among these proteins ... encodes a protein which binds to RNA and acts as a chaperone to facilitate and maintain the LINE protein-RNA complex structure ...
https://en.wikipedia.org/wiki/Short_interspersed_nuclear_element
SPTAN1SPTAN1
Moorthy S, Chen L, Bennett V (May 2000). "Caenorhabditis elegans beta-G spectrin is dispensable for establishment of epithelial ... Secondly, another insert of 20 amino acids in the 10th spectrin repeat, termed SH3i+, contains protein kinase A and protein ... Herrmann H, Wiche G (1987). "Plectin and IFAP-300K are homologous proteins binding to microtubule-associated proteins 1 and 2 ... Ankyrin repeats of the multidomain Shank protein family interact with the cytoskeletal protein alpha-fodrin". J. Biol. Chem. ...
https://en.wikipedia.org/wiki/SPTAN1
Sex-chromosome dosage compensationSex-chromosome dosage compensation
"A ONECUT homeodomain protein communicates X chromosome dose to specify Caenorhabditis elegans sexual fate by repressing a sex ... For example, in Caenorhabditis elegans (or C. elegans), sex is determined by the ratio of X chromosomes relative to autosomes; ... elegans. In fact, xol-1 is often referred to in the literature as the master sex regulatory gene of C. elegans. XX C. elegans ... Male specific lethal proteins (MSLs) are a family of four proteins that bind to the X chromosome exclusively in males. The name ...
https://en.wikipedia.org/wiki/Sex-chromosome_dosage_compensation
MicrotubuleMicrotubule
Chalfie M, Thomson JN (1979). "Organization of neuronal microtubules in the nematode Caenorhabditis elegans". Journal of Cell ... MAP-1 proteins consists of a set of three different proteins: A, B and C. The C protein plays an important role in the ... including the motor proteins dynein and kinesin, microtubule-severing proteins like katanin, and other proteins important for ... Plus end tracking proteins are MAP proteins which bind to the tips of growing microtubules and play an important role in ...
https://en.wikipedia.org/wiki/Microtubule
Leonard P. GuarenteLeonard P. Guarente
Tissenbaum, H.A.; L. Guarente (2001). "Increased dosage of a sir-2 gene extends lifespan in Caenorhabditis elegans". Nature. ... In 2000 the Guarente laboratory published work identifying SIR2's activity as a NAD+-dependent protein deacetylase. This NAD ... In round worm, Guarente reported that Caenorhabditis elegans, expression of SIR2 (sir2.1) is sufficient to extend longevity and ... Caenorhabditis elegans), and mice. He is a Novartis Professor of Biology at the Massachusetts Institute of Technology. Leonard ...
https://en.wikipedia.org/wiki/Leonard_P._Guarente
Nuclear receptorNuclear receptor
There are 270 nuclear receptors in the roundworm Caenorhabditis elegans alone, 21 in the fruit fly and other insects, 73 in ... to nuclear receptors induces a conformation of the receptor that preferentially binds coactivator proteins. These proteins ... "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-1178. Bibcode: ... Additional proteins including RNA polymerase are then recruited to the NR/DNA complex that transcribe DNA into messenger RNA. ...
https://en.wikipedia.org/wiki/Nuclear_receptor
Biomolecular condensateBiomolecular condensate
The droplets can also grow to be many molecules across (micrometres) Studies of droplets of the Caenorhabditis elegans protein ... Another example of liquid droplets in cells are the germline P granules in Caenorhabditis elegans. These granules separate out ... In addition to protein recruitment, condensates can also be designed which release proteins in response to certain stimuli. In ... Membrane protein, or membrane-associated protein, clustering at neurological synapses, cell-cell tight junctions, or other ...
https://en.wikipedia.org/wiki/Biomolecular_condensate
BZIP intron RNA motifBZIP intron RNA motif
... mouse and Caenorhabditis elegans, fruit fly, honey bee, carp, whiteleg shrimp Other Fungi: Trichoderma reesei and Aspergillus ... two proteins involved in the unfolded protein response of Litopenaeus vannamei". Developmental & Comparative Immunology. 38 (1 ... Splicing of the bZIP intron is a key regulatory step in the unfolded protein response (UPR). The Ire-mediated unconventional ... Sidrauski C, Cox JS, Walter P (1996). "tRNA ligase is required for regulated mRNA splicing in the unfolded protein response". ...
https://en.wikipedia.org/wiki/BZIP_intron_RNA_motif
TeneurinTeneurin
Ten-m refers to tenascin-like protein major. Teneurins are highly conserved between Drosophila, C. elegans and vertebrates. In ... the teneurin family is conserved from Caenorhabditis elegans (ten-1) to vertebrates, in which four paralogs exist (teneurin-1 ... and neuronal pathfinding in Caenorhabditis elegans". Developmental Biology. 282 (1): 27-38. doi:10.1016/j.ydbio.2005.02.017. ... The intracellular domains of Ten-a, Ten-m/Odz and C. elegans TEN-1 are significantly different, both in size and structure, ...
https://en.wikipedia.org/wiki/Teneurin
CoroninCoronin
The coronin-like proteins from budding yeast Crn1 and one of the coronins in Caenorhabditis elegans has a much longer unique ... Caenorhabditis elegans POD-1 and Drosophila coronin homologue regulate the actin cytoskeleton and are involved in vesicular ... Coronin proteins are expressed in a large number of eukaryotic organisms from yeast to humans. Initially, a 55 kDa protein was ... Initially this protein was admitted into club of actin binding proteins with least enthusiasm, as the primary structure did not ...
https://en.wikipedia.org/wiki/Coronin
Pre-replication complexPre-replication complex
Proteolytic regulation of Cdt1 is shared by higher eukaryotes including Caenorhabditis elegans, Drosophila melanogaster, X. ... In most eukaryotes it is composed of six ORC proteins (ORC1-6), Cdc6, Cdt1, and a heterohexamer of the six MCM proteins (MCM2-7 ... There is a stoichiometric excess of the MCM proteins over the ORC and Cdc6 proteins, indicating that there may be multiple MCM ... The ORC4 protein is known to bind the AT-rich portion of the origin of replication in S. pombe using AT hook motifs. The ...
https://en.wikipedia.org/wiki/Pre-replication_complex
ConnectomeConnectome
Yamamoto, William S.; Achacoso, Theodore B. (1992-06-01). "Scaling up the nervous system of Caenorhabditis elegans: Is one ape ... a combinatorial color labeling method based on the stochastic expression of several fluorescent proteins, Jeff W. Lichtman and ... The first (and so far only) fully reconstructed connectome belongs to the roundworm Caenorhabditis elegans. The major effort ... "The structure of the nervous system of the nematode Caenorhabditis elegans". Philosophical Transactions of the Royal Society of ...
https://en.wikipedia.org/wiki/Connectome
PIKFYVEPIKFYVE
"The phosphoinositide kinase PIKfyve/Fab1p regulates terminal lysosome maturation in Caenorhabditis elegans". Molecular Biology ... By directly binding membrane PtdIns(3)P, the FYVE finger domain of PIKfyve is essential in localizing the protein to the ... December 2008). "VAC14 nucleates a protein complex essential for the acute interconversion of PI3P and PI(3,5)P(2) in yeast and ... The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Research. 6 (1): 63-70. ...
https://en.wikipedia.org/wiki/PIKFYVE
EpigenomicsEpigenomics
Some organisms like Caenorhabditis elegans have not been demonstrated to have 5mC nor a conventional DNA methyltransferase; ... Regulatory proteins that bind to DNA, RNA, and/or proteins are key effectors in these processes and function by positively or ... The SWI/SNF protein complex in yeast is one example of a chromatin remodeling complex that regulates the expression of many ... Binding of these proteins recruit histone deacetylases (HDACs) enzyme which initiate chromatin remodeling such that the DNA ...
https://en.wikipedia.org/wiki/Epigenomics
BioGRIDBioGRID
Caenorhabditis elegans (nematode worm) Candida albicans SC5314 Canis familiaris (dog) Cavia porcellus (guinea pig) ... The BioGRID's original focus was on curation of binary protein-protein and genetic interactions, but has expanded over several ... The Biological General Repository for Interaction Datasets (BioGRID) is a curated biological database of protein-protein ... A comprehensive biomedical resource of curated protein, genetic, and chemical interactions". Protein Science. 30 (1): 187-200. ...
https://en.wikipedia.org/wiki/BioGRID
Kinesin-like protein KIF11Kinesin-like protein KIF11
Bishop JD, Han Z, Schumacher JM (2005). "The Caenorhabditis elegans Aurora B kinase AIR-2 phosphorylates and is required for ... Kinesin-like protein KIF11 is a molecular motor protein that is essential in mitosis. In humans it is coded for by the gene ... "KIF11 - Kinesin-like protein KIF11 - Homo sapiens (Human) - KIF11 gene & protein". www.uniprot.org. Retrieved 10 April 2022. ... The unique assembly of Kinesin-5 proteins not only organizes the protein complex for a different cellular function ( ...
https://en.wikipedia.org/wiki/Kinesin-like_protein_KIF11
Mediator (coactivator)Mediator (coactivator)
In both human cells and Caenorhabditis elegans MED15 is involved in lipid homeostasis through the pathway involving SREBPs In ... which may contribute to the conformational flexibility seen both with and without other bound proteins or protein complexes. A ... Fungal-specific Protein-name in Sch. pombe Kelleher RJ, Flanagan PM, Kornberg RD (June 1990). "A novel mediator between ... Micro RNAs are involved in regulating the expression of many proteins. Med1 is targeted by miR-1, which is important in gene ...
https://en.wikipedia.org/wiki/Mediator_(coactivator)
Cdc14Cdc14
The nematode Caenorhabditis elegans makes one Cdc14 (CeCdc14), which localizes to the spindle and centrosomes in mitosis, and ... "The structure of the cell cycle protein Cdc14 reveals a proline-directed protein phosphatase". EMBO J. 22 (14): 3524-3535. doi: ... "The CeCDC-14 phosphatase is required for cytokinesis in the Caenorhabditis elegans embryo". Journal of Cell Biology. 158 (5): ... Also, while the protein regulates the Cdk1 ortholog of S. pombe, this occurs through a process unlike that of S. cerevisiae; it ...
https://en.wikipedia.org/wiki/Cdc14
POU4F1POU4F1
... and the Caenorhabditis elegans factor Unc86, and named Brn3. When multiple members of the Brn3 gene class were discovered, it ... homeobox/POU domain protein RDC-1 or Oct-T1 is a protein that in humans is encoded by the POU4F1 gene. BRN3A (POU4F1) is a ... The protein product is still frequently referred to as Brn3a. In addition to sensory neurons, in rodents and birds (and ... POU4F1+protein,+human at the US National Library of Medicine Medical Subject Headings (MeSH) This article incorporates text ...
https://en.wikipedia.org/wiki/POU4F1
MechanoreceptorMechanoreceptor
Driscoll, Monica; Chalfie, Martin (February 1991). "The mec-4 gene is a member of a family of Caenorhabditis elegans genes that ... amiloride-sensitive ion channel protein related to epithelial sodium channels (ENaCs). This protein, called MEC-4, forms a ... Early research showed that touch transduction in the nematode Caenorhabditis elegans was found to require a two transmembrane, ... Mechanoreceptor proteins are ion channels whose ion flow is induced by touch. ...
https://en.wikipedia.org/wiki/Mechanoreceptor
Vault (organelle)Vault (organelle)
Caenorhabditis elegans-a free-living nematode that lives in soil. Drosophila melanogaster-a two-winged insect also known as a ... The protein structure consists of an outer shell composed of 78 copies of the ~100 kDa major vault protein (MVP). Inside are ... Proteins and peptides can also be packaged into vaults by attachment of a packaging domain derived from the VPARP protein. A ... The three vault proteins (MVP, VPARP, and TEP1) have each been knocked out individually and in combination (VPARP and TEP1) in ...
https://en.wikipedia.org/wiki/Vault_(organelle)
CUTC (gene)CUTC (gene)
2000). "Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics". ... Copper homeostasis protein cutC homolog is a protein that in humans is encoded by the CUTC gene. GRCh38: Ensembl release 89: ... 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173-8. Bibcode: ... "Identification and characterization of a novel Cut family cDNA that encodes human copper transporter protein CutC". Biochem ...
https://en.wikipedia.org/wiki/CUTC_(gene)
Rapid Degradation of Caenorhabditis elegans Proteins at Single-Cell Resolution with a Synthetic AuxinRapid Degradation of Caenorhabditis elegans Proteins at Single-Cell Resolution with a Synthetic Auxin
The auxin-inducible degradation system allows for spatial and temporal control of protein degradation via a hormone-inducible , ... Rapid Degradation of Caenorhabditis elegans Proteins at Single-Cell Resolution with a Synthetic Auxin G3 (Bethesda). 2020 Jan 7 ... tagged proteins for proteasomal degradation. Here, we optimize the Caenorhabditis elegans AID system by utilizing 1- ... We provide insight into how the AID system functions in C. elegans by determining that TIR1 depends on C. elegans SKR-1/2, CUL- ...
https://pubmed.ncbi.nlm.nih.gov/31727633/
Changes of Protein Turnover in Aging Caenorhabditis elegans* - Molecular & Cellular ProteomicsChanges of Protein Turnover in Aging Caenorhabditis elegans* - Molecular & Cellular Proteomics
The nematode Caenorhabditis elegans (C. elegans). 1. The abbreviations used are: C. elegans, Caenorhabditis elegans; E. coli, ... Increased protein stability and decreased protein turnover in the Caenorhabditis elegans Ins/IGF-1 daf-2 mutant.. J. Gerontol. ... Increased protein stability and decreased protein turnover in the Caenorhabditis elegans Ins/IGF-1 daf-2 mutant. ... Monitoring newly synthesized proteins over the adult life span of Caenorhabditis elegans.. J Proteome Res. 2015; 14: 1483-1494 ...
https://www.mcponline.org/article/S1535-9476
RCSB PDB - 1PUL: Solution structure for the 21KDa caenorhabditis elegans protein CE32E8.3. NORTHEAST STRUCTURAL GENOMICS...RCSB PDB - 1PUL: Solution structure for the 21KDa caenorhabditis elegans protein CE32E8.3. NORTHEAST STRUCTURAL GENOMICS...
Solution structure for the 21KDa caenorhabditis elegans protein CE32E8.3. NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET WR33 ... Hypothetical protein C32E8.3 in chromosome I. A. 125. Caenorhabditis elegans. Mutation(s): 0 Gene Names: C32E8.3, tppp-1. ... Backbone 1H, 15N and 13C assignments for the 21 kDa Caenorhabditis elegans homologue of "brain-specific" protein.. Monleon, D. ... Solution structure for the 21KDa caenorhabditis elegans protein CE32E8.3. NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET WR33 ...
https://www.rcsb.org/structure/1PUL
HIF-1-dependent regulation of lifespan in Caenorhabditis elegans by the acyl-CoA-binding protein MAA-1 - Institut de Génomique...HIF-1-dependent regulation of lifespan in Caenorhabditis elegans by the acyl-CoA-binding protein MAA-1 - Institut de Génomique...
HIF-1-dependent regulation of lifespan in Caenorhabditis elegans by the acyl-CoA-binding protein MAA-1. M. Shamalnasab, M. ... HIF-1-dependent regulation of lifespan in Caenorhabditis elegans by the acyl-CoA-binding protein MAA-1 ...
http://igfl.ens-lyon.fr/equipes/publications/RN1116
Fixation and Immunostaining of Endogenous Proteins or Post-translational Modifications in Caenorhabditis elegans -BIO-PROTOCOLFixation and Immunostaining of Endogenous Proteins or Post-translational Modifications in Caenorhabditis elegans -BIO-PROTOCOL
... elegans ciliated sensory neurons and to detect non-modified endogenous protein (Topalidou and Chalfie, 2011). ... Fluorescently-tagged proteins created by CRISPR genome editing are less prone to defective expression patterns because the loci ... Here, we present a reliable protocol in which C. elegans nematodes are fixed, preserved, and permeabilized for staining with a ... if the fluorescent tag obstructs or otherwise interferes with important protein interaction domains or affects the protein ...
https://bio-protocol.org/e4172
Joubert syndrome Arl13b functions at ciliary membranes and stabilizes protein transport in Caenorhabditis elegans | AVESİSJoubert syndrome Arl13b functions at ciliary membranes and stabilizes protein transport in Caenorhabditis elegans | AVESİS
Joubert syndrome Arl13b functions at ciliary membranes and stabilizes protein transport in Caenorhabditis elegans ... using Caenorhabditis elegans and mammalian cell culture systems, we investigated the poorly understood ciliary and molecular ... as well as defects in ciliary protein localization and transport; ciliary transmembrane proteins abnormally accumulate, PKD-2 ... The small ciliary G protein Arl13b is required for cilium biogenesis and sonic hedgehog signaling and is mutated in patients ...
https://avesis.agu.edu.tr/yayin/01136b82-2933-46e8-a78b-be7cfa603845/joubert-syndrome-arl13b-functions-at-ciliary-membranes-and-stabilizes-protein-transport-in-caenorhabditis-elegans
IUCr) Acta Crystallographica Section D Volume 60, Part 10, October 2004IUCr) Acta Crystallographica Section D Volume 60, Part 10, October 2004
Structure of sperm-specific protein SSP-19 from Caenorhabditis elegans. N. Schormann, J. Symersky and M. Luo ... The crystals of recombinant protein expressed in E. coli belong to space group P21212, with unit-cell parameters a = 150.5, b ... YciE protein from E. coli was overexpressed and purified. Crystals of YciE belonged to space group R32 and diffracted to a ... The protein has been crystallized in the orthorhombic space group P2221 and the crystals diffract to 2.7 Å resolution. ...
https://journals.iucr.org/d/issues/2004/10/00/
The two Caenorhabditis elegans actin-depolymerizing factor/cofilin proteins differently enhance actin filament severing and...
The two Caenorhabditis elegans actin-depolymerizing factor/cofilin proteins differently enhance actin filament severing and ... The two Caenorhabditis elegans actin-depolymerizing factor/cofilin proteins differently enhance actin filament severing and ... The two Caenorhabditis elegans actin-depolymerizing factor/cofilin proteins differently enhance actin filament severing and ... T1 - The two Caenorhabditis elegans actin-depolymerizing factor/cofilin proteins differently enhance actin filament severing ...
https://tohoku.pure.elsevier.com/en/publications/the-two-caenorhabditis-elegans-actin-depolymerizing-factorcofilin
The Caenorhabditis elegans cell-death protein CED-3 is a cysteine protease with substrate specificities similar to those of the...The Caenorhabditis elegans cell-death protein CED-3 is a cysteine protease with substrate specificities similar to those of the...
The Caenorhabditis elegans cell-death protein CED-3 is a cysteine protease with substrate specificities similar to those of the ... Biochemical Phenomena - Protein Processing, Post-Translational * Biochemical Phenomena - Protein Processing, Post-Translational ...
https://experts.colorado.edu/display/pubid_77333
A Caenorhabditis elegans protein with a PRDM9-like SET domain localizes to chromatin-associated foci and promotes spermatocyte...
keywords = "Animals, Caenorhabditis elegans/enzymology, Caenorhabditis elegans Proteins/genetics, Chromatin/metabolism, ... Engert, C. G., Droste, R., van Oudenaarden, A., & Horvitz, H. R. (2018). A Caenorhabditis elegans protein with a PRDM9-like SET ... A Caenorhabditis elegans protein with a PRDM9-like SET domain localizes to chromatin-associated foci and promotes spermatocyte ... A Caenorhabditis elegans protein with a PRDM9-like SET domain localizes to chromatin-associated foci and promotes spermatocyte ...
https://pure.knaw.nl/portal/en/publications/a-caenorhabditis-elegans-protein-with-a-prdm9-like-set-domain-loc
MetabolismMetabolism
... is mediated by AICAR-transformylase/IMP cyclohydrolase and mitigated by AMP-activated protein kinase in Caenorhabditis elegans ... AMP-activated Protein Kinase Up-regulates Mitogen-activated Protein (MAP) Kinase-interacting Serine/Threonine Kinase 1a- ... Activation of AMP-activated Protein Kinase by Metformin Induces Protein Acetylation in Prostate and Ovarian Cancer Cells. ... Nischarin (Nisch) is a key protein functioning as a molecular scaffold and thereby hosting interactions with several protein ...
https://www.jbc.org/Metabolism?startPage=&KeywordRaw=AMP-activated%20kinase%20
Frontiers | Hemichannel composition and electrical synaptic transmission: molecular diversity and its implications for...Frontiers | Hemichannel composition and electrical synaptic transmission: molecular diversity and its implications for...
Unapposed hemichannels formed by oligomerized hexamers of gap junction proteins are now known to be involved in various ... formed by hexamers of gap junction proteins are now known to be involved in various cellular processes under both physiological ... The Caenorhabditis elegans avermectin resistance and anesthetic response gene unc-9 encodes a member of a protein family ... High resolution map of Caenorhabditis elegans gap junction proteins. Dev. Dyn. 238, 1936-1950. doi: 10.1002/dvdy.22025 ...
https://www.frontiersin.org/articles/10.3389/fncel.2014.00324/full
Biblio | Journal of Alzheimers DiseaseBiblio | Journal of Alzheimer's Disease
Caenorhabditis elegans Proteins. T. Lu, Aron, L., Zullo, J., Pan, Y., Kim, H., Chen, Y., Yang, T. - H., Kim, H. - M., Drake, D. ... Repressor Proteins. T. Lu, Aron, L., Zullo, J., Pan, Y., Kim, H., Chen, Y., Yang, T. - H., Kim, H. - M., Drake, D., X Liu, S., ... DNA-Binding Proteins. T. Lu, Aron, L., Zullo, J., Pan, Y., Kim, H., Chen, Y., Yang, T. - H., Kim, H. - M., Drake, D., X Liu, S. ...
https://www.j-alz.com/biblio?f%5Bauthor%5D=1913&s=keyword&o=desc
Kindler epidermolysis bullosa: MedlinePlus GeneticsKindler epidermolysis bullosa: MedlinePlus Genetics
Loss of kindlin-1, a human homolog of the Caenorhabditis elegans actin-extracellular-matrix linker protein UNC-112, causes ... This gene provides instructions for making a protein known as kindlin-1. This protein is found in epithelial cells, which are ... Most variants in the FERMT1 gene prevent the production of any functional kindlin-1. A lack of this protein disrupts many ... Recurrent mutations in kindlin-1, a novel keratinocyte focal contact protein, in the autosomal recessive skin fragility and ...
https://medlineplus.gov/genetics/condition/kindler-syndrome/
positive regulation of hypodermal cell differentiation | Semantic Scholarpositive regulation of hypodermal cell differentiation | Semantic Scholar
The structural proteins of the cytoplasmic intermediate filaments (IFs) arise in the nematode Caenorhabditis elegans from eight ... Essential roles for four cytoplasmic intermediate filament proteins in Caenorhabditis elegans development ...
https://www.semanticscholar.org/topic/positive-regulation-of-hypodermal-cell/10915192
Category:Chromatin - Wikimedia CommonsCategory:Chromatin - Wikimedia Commons
LeptoteneZygotene-Chromosome-Movement-Via-the-SUNKASH-Protein-Bridge-in-Caenorhabditis-elegans-pgen.1001219.s014.ogv 12 s, 656 ... Histone-H3.3-Variant-Dynamics-in-the-Germline-of-Caenorhabditis-elegans-pgen.0020097.sv001.ogv 1 min 0 s, 1,024 × 512; 1.91 MB ... Histone-H3.3-Variant-Dynamics-in-the-Germline-of-Caenorhabditis-elegans-pgen.0020097.sv002.ogv 6.7 s, 1,024 × 512; 783 KB. ... Nuclear-Pore-Proteins-Nup153-and-Megator-Define-Transcriptionally-Active-Regions-in-the-Drosophila-pgen.1000846.s017.ogv 7.2 s ...
https://commons.wikimedia.org/wiki/Category:Chromatin
The SensorOverlord predicts the accuracy of measurements with ratiometric biosensors | Scientific ReportsThe SensorOverlord predicts the accuracy of measurements with ratiometric biosensors | Scientific Reports
Regulated spatial organization and sensitivity of cytosolic protein oxidation in Caenorhabditis elegans. Nat. Commun. 5, 5020. ... These protein networks can be understood as markets where cysteines in proteins buy (reduction) and sell (oxidation) pairs of ... Cannon, M. B. & Remington, S. J. Re-engineering redox-sensitive green fluorescent protein for improved response rate. Protein ... We predicted the EGSH inaccuracy that we would observe if we measured EGSH in the feeding muscles of live C. elegans with the ...
https://www.nature.com/articles/s41598-020-73987-0?error=cookies_not_supported
Sol Genomics Network
Reactome:REACT_34480 "G-protein alpha subunit is inactivated, Caenorhabditis elegans". Reactome:REACT_34592 "G alpha (i) auto- ... heterotrimeric G-protein GTPase activity NARROW heterotrimeric G-protein GTPase, alpha-subunit RELATED heterotrimeric G-protein ... protein-synthesizing GTPase activity NARROW protein-synthesizing GTPase activity, elongation NARROW protein-synthesizing GTPase ... "protein-synthesizing GTPase activity" NARROW [] "protein-synthesizing GTPase activity, elongation" NARROW [] "protein- ...
https://www.sgn.cornell.edu/cvterm/1360/view
Thomas M. Roberts, PhD - Dana-Farber Cancer Institute | Boston, MAThomas M. Roberts, PhD - Dana-Farber Cancer Institute | Boston, MA
2.6 A resolution crystal structure of helices of the motile major sperm protein (MSP) of Caenorhabditis elegans. J Mol Biol. ... Dephosphorylation of major sperm protein (MSP) fiber protein 3 by protein phosphatase 2A during cell body retraction in the MSP ... Interaction of the protein kinase Raf-1 with 14-3-3 proteins. Science. 1994 Oct 07; 266(5182):126-9.. View in: PubMed. ... The third subunit of protein phosphatase 2A (PP2A), a 55-kilodalton protein which is apparently substituted for by T antigens ...
https://www.dana-farber.org/find-a-doctor/thomas-m-roberts/
spp-12 gene cDNA ORF clone, Caenorhabditis elegans - GenScriptspp-12 gene cDNA ORF clone, Caenorhabditis elegans - GenScript
Next-day shipping cDNA ORF clones derived from spp-12 SaPosin-like Protein family available at GenScript, starting from $99.00. ... spp-12 ( NM_074042.1 ) cDNA ORF clone, Caenorhabditis elegans -, NP_506443.1 Caenorhabditis elegans SaPosin-like Protein family ... Caenorhabditis elegans -, NP_506443.1 Caenorhabditis elegans Saposin B-type domain-containing protein (spp-12), mRNA. ... Caenorhabditis elegans Saposin B-type domain-containing protein (spp-12), mRNA.. pcDNA3.1-C-(k)DYK or customized vector. 9-11. ...
https://www.genscript.com/gene/caenorhabditis-elegans/188762/spp-12.html
Lai F et al. (2012), Xenopus Nanos1 is required to prevent endoderm ... - Paper Lai F et al. (2012), Xenopus Nanos1 is required to prevent endoderm ... - Paper
Transcriptional repression by the Caenorhabditis elegans germ-line protein PIE-1. 1999, Pubmed Bouwmeester, Cerberus is a head- ... A conserved RNA-binding protein that regulates sexual fates in the C. elegans hermaphrodite germ line. 1997, Pubmed Zhang, The ... Control of the sperm-oocyte switch in Caenorhabditis elegans hermaphrodites by the fem-3 3 untranslated region. 1991, Pubmed ... Control of the sperm-oocyte switch in Caenorhabditis elegans hermaphrodites by the fem-3 3 untranslated region. 1991, Pubmed ...
https://www.xenbase.org/entry/literature/article.do?method=display&articleId=44949
Fabio PianoFabio Piano
Caenorhabditis elegans decapping proteins: localization and functional analysis of Dcp1, Dcp2, and DcpS during embryogenesis.. ... A protein domain-based interactome network for C. elegans early embryogenesis.. Cell (2008 Aug 8) PMC2596478 free full-text ... A model of cytoplasmically driven microtubule-based motion in the single-celled Caenorhabditis elegans embryo.. Proc Natl Acad ... mRNA capping enzyme requirement for Caenorhabditis elegans viability.. J Biol Chem (2003 Apr 18) PMID: 12576475. Srinivasan P, ...
https://as.nyu.edu/faculty/fabio-piano.html
Anti-6X His tag® antibody [4D11] (ab5000) | AbcamAnti-6X His tag® antibody [4D11] (ab5000) | Abcam
Stephens AD et al. Isolation and Imaging of His- and RFP-tagged Amyloid-like Proteins from Caenorhabditis elegans by TEM and ... Proteins and Peptides. By product type. Proteomics tools. Agonists, activators, antagonists and inhibitors. Cell lines and ... The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the ... Beta3-His fusion protein was immunoprecipitated using anti His Ab, and specific beta3a band (56kDa) was detected using anti ...
https://www.abcam.com/6x-his-tag-antibody-4d11-ab5000.html
Neurotree - Ann RougvieNeurotree - Ann Rougvie
2006) Novel heterochronic functions of the Caenorhabditis elegans period-related protein LIN-42. Developmental Biology. 289: 30 ... 2014) Caenorhabditis elegans period homolog lin-42 regulates the timing of heterochronic miRNA expression. Proceedings of the ... 2016) Analysis of a lin-42/Period Null Allele Implicates All Three Isoforms in Regulation of Caenorhabditis elegans Molting and ... 2010) miRNAs give worms the time of their lives: small RNAs and temporal control in Caenorhabditis elegans. Developmental ...
https://neurotree.org/neurotree/peopleinfo.php?pid=27790
Sandwalk: September 2008Sandwalk: September 2008
The latest estimate is 20,140 protein coding genes in the Caenorhabditis elegans genome. The coding regions (exons) would take ... Protein interactions will not be solved by proteomics or protein chips but by protein biochemistry. The genome sequences tell ... The Pristionchus pacificus genome is 169 Mb in size, which is considerably larger than the size of the Caenorhabditis elegans ... The free-living species Caenorhabditis elegans was chosen by Sydney Brenner as a model organism for the study of development [ ...
https://sandwalk.blogspot.com/2008/09/
Vitamin E Does Not Cause Prostate Cancer | Baseline of Health FoundationVitamin E Does Not Cause Prostate Cancer | Baseline of Health Foundation
Adachi H, Ishii N. "Effects of tocotrienols on life span and protein carbonylation in Caenorhabditis elegans." J Gerontol A ... "Effects of tocotrienols on life span and protein carbonylation in Caenorhabditis elegans." J Gerontol A Biol Sci Med Sci. 2000 ...
https://www.jonbarron.org/article/vitamin-e-does-not-cause-prostate-cancer
Noble Research Lab: Publications and software
"Visualization and dissemination of multidimensional proteomics data comparing protein abundance during Caenorhabditis elegans ... "Learning to predict protein-protein interactions from protein sequences." Bioinformatics (Proceedings of the Georgia Tech ... "Protein interaction networks: protein domain interaction and protein function prediction." In Handbook of Statistical ... "Protein ranking: from local to global structure in the protein similarity network." Proceedings of the National Academy of ...
https://noble.gs.washington.edu/pubs.html
NematodeGenes2018GenomeGermlineSpeciesTransgenicStrains2016ChromatinNematodesHomologDrosophilaRegulationSoluble and membrane proteinsGreen fluoresLifespanRegulatesMammalianTargeted protein degradationMolecular BiologyMutationIntrinsically disorderedRNAsIsoformsDegradationBiologyAmino AcidsClonesCellularMoleculesKinaseDynamicsExperimentsDomain-containMembrane-proteinNspsAlpha-synucleinHomeostasisRibonucleoproteinRNAiActin cytoskeletonBindTubulinWormsStructuralSubunitVivoTransmembrane proteinModelDevelopmentDomainsDefectsGeneticInteractExpression
Nematode7
  • Genome sequence of the nematode C. elegans: a platform for investigating biology. (genscript.com)
  • The nematode C. elegans can take up Moco from its bacterial diet and transport it to cells and tissues that express Moco-requiring enzymes, suggesting a system for Moco uptake and distribution," noted the investigators. (genengnews.com)
  • We tested four proteins and all four were restorative in our nematode model. (genengnews.com)
  • Proteins from the nematode species CAENORHABDITIS ELEGANS . (bvsalud.org)
  • We developed efficient procedures for targeted, heritable disruption of genes and cis-acting regulatory elements in the model nematode Caenorhabditis elegans and applied them to C. briggsae , a species diverged by 15 to 30 million years. (bioseek.eu)
  • Using the nematode Caenorhabditis elegans they show that the inheritance of small RNAs antisense to histone genes adversely affect the fertility of worms across generations until they become sterile. (pasteur.fr)
  • However, in the nematode Caenorhabditis elegans piRNA mutants are initially fertile and become sterile only after many generations. (pasteur.fr)
Genes6
  • To better understand the tissue-specific regulation of chromatin state in cell-fate determination and animal development, we defined the tissue-specific expression of all 36 C. elegans presumptive lysine methyltransferase (KMT) genes using single-molecule fluorescence in situ hybridization (smFISH). (knaw.nl)
  • Resnick T, Malmquist S, Rougvie A. ( 2009 ) Developmental timing genes identified through miRNA suppressor screens in C. elegans Developmental Biology . (neurotree.org)
  • The discovery of specific genes and proteins associated with AD, and the development of new technologies for the production of transgenic animals, has helped researchers to overcome the lack of natural models. (springer.com)
  • Their study, " Protein-bound molybdenum cofactor is bioavailable and rescues molybdenum cofactor-deficient C. elegans, " is published in the journal Genes and Development . (genengnews.com)
  • The researchers investigated the interactions between the transcription factor DAF-16 and the genes that regulate the production of an insulin-like growth factor 1 (IGF-1-like) protein related to the development, reproduction, and aging in C. elegans. (asu.edu)
  • Lai CH, Chou CY, Ch'ang LY, Liu CS, Lin W: Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics. (smpdb.ca)
20181
  • Care4Rare Canada Consortium, Boycott KM, Bastin P and Sheridan EG (2018) Biallelic Mutations in LRRC56, Encoding a Protein Associated with Intraflagellar Transport, Cause Mucociliary Clearance and Laterality Defects. (sfu.ca)
Genome8
  • Fluorescently-tagged proteins created by CRISPR genome editing are less prone to defective expression patterns because the loci retain endogenous DNA elements that regulate their transcription (Nance and Frøkjær-Jensen, 2019). (bio-protocol.org)
  • Therefore, our data can be used not only to analyze the C. elegans genome but also to guide the functional examination of the human genome. (nyu.edu)
  • We have recently sequenced the genome of a parthogenetic relative of C. elegans that shows an alternate pattern of early cleavages. (nyu.edu)
  • Recent advances in genome editing have expedited the precise insertion of fluorescent protein tags into the genomes of diverse organisms. (cam.ac.uk)
  • Check out our detailed JoVE protocol and accompanying video walkthrough for genome editing with CRISPR-Cas9 RNPs in primary human HSPCs and T cells, Caenorhabditis elegans , and Parhyale hawaiensis . (innovativegenomics.org)
  • 2] Dickinson DJ, And Goldstein B. (2016) CRISPR-based methods for Caenorhabditis elegans genome engineering. (wormbook.org)
  • 4] Paix A, Kolkmann A, Rasoloson D, and Seydoux G. (2015) High efficiency, homology-directed genome editing in Caenorhabditis elegans using CRISPR-Cas9 ribonucleoprotein complexes. (wormbook.org)
  • Assignment of Homology to Genome Sequences using a Library of Hidden Markov Models that Represent all Proteins of Known Structure. (cam.ac.uk)
Germline5
  • We found that the germline-expressed C. elegans protein SET-17, which has a SET domain similar to that of the PRDM9 and PRDM7 SET-domain proteins, promotes fertility by regulating gene expression in primary spermatocytes. (knaw.nl)
  • Developmental dynamics of gene expression and alternative polyadenylation in the Caenorhabditis elegans germline. (nyu.edu)
  • Now, on p. 4975 , Boag and co-workers report that a conserved RNA-protein (RNP)complex regulates germline apoptosis in Caenorhabditis elegans . (biologists.com)
  • They identify a germline RNA-binding protein, CAR-1 (for cytokinesis/apoptosis/RNA binding), and show that it associates with the RNA helicase CGH-1 in an RNA-dependent manner within a germline RNP complex. (biologists.com)
  • LOTUS and Tudor domain containing proteins have critical roles in the germline. (bvsalud.org)
Species3
  • Significantly, in some species the wild-type patterns of early embryonic cell divisions resemble those produced by various C. elegans mutants, indicating plasticity in the underlying molecular networks. (nyu.edu)
  • Here we show that protein-bound Moco is the stable, bioavailable species of Moco taken up by C. elegans from its diet and is an effective dietary supplement, rescuing a C. elegans model of Moco deficiency. (genengnews.com)
  • The proteins from this species are the subject of scientific interest in the area of multicellular organism MORPHOGENESIS . (bvsalud.org)
Transgenic3
  • We generated transgenic Caenorhabditis elegans strains expressing green, yellow, or red fluorescent proteins in embryos and imaged embryos expressing different fluorescent proteins under the same conditions for direct comparison. (cam.ac.uk)
  • Efficient generation of transgenic reporter strains and analysis of expression patterns in Caenorhabditis elegans using library MosSCI. (harvard.edu)
  • We developed a fluorescence-based in vivo assay using transgenic Caenorhabditis elegans worms that express the green fluorescent protein (GFP) under the control of various CYP promoters. (who.int)
Strains1
  • Isolated Desulfovibrio strains were fed to a strain of Caenorhabditis elegans roundworms that expressed human alpha-syn fused with yellow fluorescent protein. (medscape.com)
20161
  • 2016 ) Analysis of a lin-42/Period Null Allele Implicates All Three Isoforms in Regulation of Caenorhabditis elegans Molting and Developmental Timing. (neurotree.org)
Chromatin1
  • SET-17 is concentrated in stable chromatin-associated nuclear foci at actively transcribed msp (major sperm protein) gene clusters, which we term msp locus bodies. (knaw.nl)
Nematodes3
  • Here, we present a reliable protocol in which C. elegans nematodes are fixed, preserved, and permeabilized for staining with a primary antibody to bind proteins or post-translational modifications, which are then labeled with a secondary antibody conjugated to a fluorescent dye. (bio-protocol.org)
  • We study the genetic and evolutionary mechanisms underlying early embryonic development using a combination of molecular genetic and functional genomics approaches in the animal model C. elegans and related nematodes. (nyu.edu)
  • In 2003, molecular biology and genetics researchers Coleen T. Murphy, Steven A. McCarroll, Cornelia I. Bargmann, Andrew Fraser, Ravi S. Kamath, Julie Ahringer, Hao Li, and Cynthia Kenyon conducted an experiment that investigated the cellular aging in, Caenorhabditis elegans (C. elegans) nematodes. (asu.edu)
Homolog3
  • Kindlin-1 is a human homolog of the Caenorhabditis elegans protein UNC-112, a membrane-associated structural/signaling protein that had been implicated in linking the actin cytoskeleton to the extracellular matrix (ECM). (medscape.com)
  • 2014 ) Caenorhabditis elegans period homolog lin-42 regulates the timing of heterochronic miRNA expression. (neurotree.org)
  • They bind and anchor the Vasa homolog GLH-1 within P granules and are jointly required for coalescence of MEG-3, GLH-1, and PGL proteins. (bvsalud.org)
Drosophila2
  • Proteins that contain these domains, such as Tejas/Tapas in Drosophila, help localize the Vasa helicase to the germ granules and facilitate piRNA-mediated transposon silencing. (bvsalud.org)
  • We show here that AIP associates with AhR homologues from mouse and fish, which can bind ligands such as dioxin, but nonligand binding homologues from Caenorhabditis elegans or Drosophila do not bind to AIP. (cdc.gov)
Regulation2
  • In C. elegans, aex-4 is involved in positive regulation of defecation and positive regulation of protein secretion. (wormbase.org)
  • a ) Glutathione redox potential ( E GSH ) directs the oxidation of cysteines in hundreds of proteins in the same direction, resulting in their concerted regulation. (nature.com)
Soluble and membrane proteins1
  • Jensen VL and Leroux MR (2017) Gates for soluble and membrane proteins, and two trafficking systems (IFT and LIFT), establish a dynamic ciliary signaling compartment. (sfu.ca)
Green fluores1
  • Using an unc-2 promoter-tagged green fluorescent protein construct, we show that unc-2 is primarily expressed in motor neurons, several subsets of sensory neurons, and the HSN and VC neurons that control egg laying. (jneurosci.org)
Lifespan3
  • Mechanism of the lifespan extension of Caenorhabditis elegans by electrolyzed reduced water-participation of Pt nanoparticles. (h2bev.com)
  • electrolyzed reduced water prolongs caenorhabditis elegans lifespan , in Animal Cell Technology: Basic & Applied Aspects . (h2bev.com)
  • Extension of the Lifespan of Caenorhabditis elegans by the Use of Electrolyzed Reduced Water. (h2bev.com)
Regulates4
  • Together, these data implicate a role for JS-associated Arl13b at ciliary membranes, where it regulates ciliary transmembrane protein localizations and anterograde IFT assembly stability. (agu.edu.tr)
  • 2010 ) The C. elegans developmental timing protein LIN-42 regulates diapause in response to environmental cues. (neurotree.org)
  • Interestingly, KLHL21 but not KLHL22 is necessary for cytokinesis and regulates translocation of the chromosomal passenger complex (CPC) from chromosomes to the spindle midzone in anaphase, similar to the previously described BTB-Kelch proteins KLHL9 and KLHL13. (rupress.org)
  • The TATA-binding protein regulates maternal mRNA degradation and differential zygotic transcription in zebrafish. (harvard.edu)
Mammalian3
  • In this study, using Caenorhabditis elegans and mammalian cell culture systems, we investigated the poorly understood ciliary and molecular basis of Arl13b function. (agu.edu.tr)
  • The Caenorhabditis elegans unc-2 gene encodes a voltage-gated calcium channel α 1 subunit structurally related to mammalian dihydropyridine-insensitive high-threshold channels. (jneurosci.org)
  • In this study, we identify two mammalian BTB (Bric-a-brac-Tramtrack-Broad complex)-Kelch proteins, KLHL21 and KLHL22, that interact with Cul3 and are required for efficient chromosome alignment. (rupress.org)
Targeted protein degradation1
  • We also demonstrate that K-NAA, the water-soluble, potassium salt of NAA, can be combined with microfluidics for targeted protein degradation in C. elegans larvae. (nih.gov)
Molecular Biology1
  • Protein Homeostasis Diseases: Mechanisms and Novel Therapies offers an interdisciplinary examination of the fundamental aspects, biochemistry and molecular biology of protein homeostasis disease, including the use of natural and pharmacological small molecules to treat common and rare protein homeostasis disorders. (elsevier.com)
Mutation1
  • Purified AIP binds to the C terminus of hsp90, and mutation of a conserved basic residue in the tetratricopeptide repeats of AIP (K266A, analogous to K97A in protein phosphatase 5) abolishes binding to hsp90. (cdc.gov)
Intrinsically disordered1
  • We describe MIP-1 and MIP-2, novel paralogous C. elegans germ granule components that interact with the intrinsically disordered MEG-3 protein. (bvsalud.org)
RNAs2
  • 2010 ) miRNAs give worms the time of their lives: small RNAs and temporal control in Caenorhabditis elegans. (neurotree.org)
  • Protection of specific maternal messenger RNAs by the P body protein CGH-1 (Dhh1/RCK) during Caenorhabditis elegans oogenesis. (harvard.edu)
Isoforms2
  • Multicellular organisms express multiple ADF/cofilin isoforms in a tissue-specific manner, and the vertebrate proteins are grouped into ADFs and cofilins on the basis of their biochemical activity. (elsevier.com)
  • Here, we show that the two Caenorhabditis elegans ADF/cofilin isoforms exhibit different activities for severing and depolymerizing actin filaments. (elsevier.com)
Degradation4
  • The auxin-inducible degradation system allows for spatial and temporal control of protein degradation via a hormone-inducible Arabidopsis F-box protein, transport inhibitor response 1 (TIR1). (nih.gov)
  • In the presence of auxin, TIR1 serves as a substrate-recognition component of the E3 ubiquitin ligase complex SKP1-CUL1-F-box (SCF), ubiquitinating auxin-inducible degron (AID)-tagged proteins for proteasomal degradation. (nih.gov)
  • We take advantage of the photostability of NAA to demonstrate via quantitative high-resolution microscopy that rapid degradation of target proteins can be detected in single cells within 30 min of exposure. (nih.gov)
  • Finally, we present highly penetrant defects from NAA-mediated degradation of the FTZ-F1 nuclear hormone receptor, NHR-25, during C. elegans uterine-vulval development. (nih.gov)
Biology1
  • We develop new tools in structural biology, namely MicroED as a new method for cryo EM, to facilitate the study of such membrane proteins to atomic resolution from vanishingly small crystals. (janelia.org)
Amino Acids1
  • I think there were only forty-five amino acids [the building blocks of proteins] that were in insulin. (elizabethdzeng.com)
Clones2
  • Repositorio consejería de sanidad de madrid: Interplay among Resistance Profiles, High-Risk Clones, and Virulence in the Caenorhabditis elegans Pseudomonas aeruginosa Infection Model. (repositoriosaludmadrid.es)
  • In this work, we attempted to decipher the interplay between resistance profiles, high-risk clones, and virulence, testing a large (n = 140) collection of well-characterized P. aeruginosa isolates from different sources (bloodstream infections, nosocomial outbreaks, cystic fibrosis, and the environment) in a Caenorhabditis elegans infection model. (repositoriosaludmadrid.es)
Cellular5
  • The cellular energy sensor AMP-activated protein kinase (AMPK) is a metabolic regulator that mediates adaptation to nutritional variations to maintain a proper energy balance in cells. (jbc.org)
  • The cellular energy sensor AMP-activated protein kinase (AMPK) is a metabolic hub regulating various pathways involved in tumor metabolism. (jbc.org)
  • Unapposed hemichannels (HCs) formed by hexamers of gap junction proteins are now known to be involved in various cellular processes under both physiological and pathological conditions. (frontiersin.org)
  • Brenner won the Nobel Prize for establishing Caenorhabditis elegans, a type of roundworm, as the model organism for cellular and developmental biological research, which led to discoveries in organ development and programmed cell death. (elizabethdzeng.com)
  • As a result, we found proteins that are involved in important processes during development, such as energy metabolism, control pathways and cellular communication. (cdc.gov)
Molecules3
  • Kindlin-1 is involved in several important cell functions, including cell growth and division (proliferation), the attachment of cells to the underlying network of proteins and other molecules (cell-matrix adhesion), and the movement (migration) of cells. (medlineplus.gov)
  • This code is the process by which cells in our body translate information stored in our DNA into proteins, vital molecules important to the structure and functioning of cells. (elizabethdzeng.com)
  • Other in silico methods that are routinely used in research laboratories include molecular modelling (a technique used to model or mimic the structure of molecules) and protein sequencing and its alignment (methods used to evaluate identities and similarities in the amino acid sequence of proteins) [25-28]. (biomedscis.com)
Kinase2
  • The structure of the regulatory subunit of the protein kinase CK2 crystallized in the presence of p21 WAF1 suggests binding in the solvent-accessible part of the zinc-finger motif. (iucr.org)
  • Cdk2, Cell division protein kinase 2 (EC 2.7.1. (reactome.org)
Dynamics4
  • However, limited information is available on turnover dynamics at the individual protein level during aging. (mcponline.org)
  • Although these proteins are biochemically distinct and play different roles in actin dynamics, they all appear to use the ADF-H domain for their interactions with actin. (embl.de)
  • Cofilin, a ubiquitous 15,000 M(r) protein, plays a central role in regulating cytoskeletal dynamics. (embl.de)
  • Fluorescent protein tags are fundamental tools used to visualize gene products and analyze their dynamics in vivo. (cam.ac.uk)
Experiments4
  • Protein expression is influenced by many factors that may vary between experiments or laboratories. (genscript.com)
  • These advances expand the potential of in vivo imaging experiments and facilitate experimentation with new, bright, photostable fluorescent proteins. (cam.ac.uk)
  • We found that mNeonGreen was not as bright in vivo as predicted based on in vitro data but is a better tag than GFP for specific kinds of experiments, and we report on optimal red fluorescent proteins. (cam.ac.uk)
  • These results identify ideal fluorescent proteins for imaging in vivo in C. elegans embryos and suggest good candidate fluorescent proteins to test in other animal model systems for in vivo imaging experiments. (cam.ac.uk)
Domain-contain3
  • and Thrombospondin type-1 domain-containing protein 1. (wormbase.org)
  • Caenorhabditis elegans Saposin B-type domain-containing protein (spp-12), mRNA. (genscript.com)
  • Chinnaiyan AM, O'Rourke K, Tewari M, Dixit VM: FADD, a novel death domain-containing protein, interacts with the death domain of Fas and initiates apoptosis. (smpdb.ca)
Membrane-protein2
  • In situ serial crystallography for rapid de novo membrane protein structure determination. (psi.ch)
  • Both proteins form a complex with synaptobrevin, an intrinsic membrane protein of SVs. (uni-bielefeld.de)
Nsps3
  • Following the entry into the host cell, SARS-CoV-2 replication is mediated by the replication transcription complex (RTC) assembled through a number of nonstructural proteins (Nsps). (bvsalud.org)
  • The in vitro incorporation of AHA with different tags into newly synthesized proteins (NSPs) by PSCs was analyzed using SDS-PAGE and confocal microscopy. (cdc.gov)
  • The LC-MS/MS analysis of AHA-labeled NSPs by PSCs undergoing strobilation allowed for the identification of 365 proteins, of which 75 were differentially expressed in comparison between the presence or absence of strobilation stimuli and 51 were expressed exclusively in either condition. (cdc.gov)
Alpha-synuclein2
  • A common gut bacteria may play a role in the development of Parkinson's disease (PD) by causing aggregation of the alpha-synuclein protein, a key feature in the pathology of PD, a small study suggests. (medscape.com)
  • Similar patterns of mitochondrial vulnerability and rescue induced by genetic modification of alpha-synuclein, parkin, and DJ-1 in Caenorhabditis elegans. (wehaveparkinsons.com)
Homeostasis3
  • Contributions from international experts discuss the biochemical and genetic components of protein homeostasis disorders, the mechanisms by which genetic variants may cause loss-of-function and gain-of-toxic-function, and how natural ligands can restore protein function and homeostasis in genetic diseases. (elsevier.com)
  • Applied chapters provide guidance on employing high throughput sequencing and screening methodologies to develop pharmacological chaperones and repurpose approved drugs to treat protein homeostasis disorders. (elsevier.com)
  • Our laboratory studies the structures of membrane proteins important in homeostasis and signaling. (janelia.org)
Ribonucleoprotein1
  • We propose that these proteins serve as scaffolds and organizing centers for ribonucleoprotein networks within P granules that help recruit and balance essential RNA processing machinery to regulate key developmental transitions in the germ line. (bvsalud.org)
RNAi2
  • One of the main tools we have used is RNA interference (RNAi) followed by time-lapse microscopy to work toward a comprehensive molecular description of early embryogenesis in C. elegans . (nyu.edu)
  • For example, expression of a constitutively active form of a tagged protein or knockdown of a target by RNAi can simulate the effects of a sought-after small molecule in a screen. (nih.gov)
Actin cytoskeleton1
  • Actin depolymerizing factors (ADF) are stimulus responsive actin cytoskeleton modulating proteins. (embl.de)
Bind2
  • Tamoxifen itself doesn't bind to the estradiol receptor protein, instead it is converted inside your body to other chemicals that do bind. (blogspot.com)
  • This binding is thought to be responsible for vesicle docking and apparently precedes membrane fusion, According to the current concept, syntaxin 1 and SNAP-25 are members of larger protein families, collectively designated as target-SNAP receptors (t-SNAREs), whose specific localization to subcellular membranes define where transport vesicles bind and fuse, Here we demonstrate that major pools of syntaxin 1 and SNAP-25 recycle with SVs. (uni-bielefeld.de)
Tubulin3
  • 2020) to preserve and detect post-translational modifications of tubulin in C. elegans ciliated sensory neurons and to detect non-modified endogenous protein (Topalidou and Chalfie, 2011). (bio-protocol.org)
  • We have identified a remarkable complex of proteins associated with a third type of tubulin, gamma-tubulin. (stanford.edu)
  • Gamma-tubulin and its associated proteins are localized to the centrosome and are critical for initiation, or nucleation, of microtubule assembly. (stanford.edu)
Worms1
  • The research team observed that the worms could take in Moco as a range of purified Moco-protein complexes. (genengnews.com)
Structural2
  • SCOPe: Structural Classification of Proteins - extended. (berkeley.edu)
  • The proteins responsible for the key molecular events leading to the structural changes between the developmental stages of Echinococcus granulosus remain unknown. (cdc.gov)
Subunit1
  • The α 1 subunit forms the voltage-sensor and channel proper, whereas the remaining proteins interact with the α 1 subunit to modulate channel activity. (jneurosci.org)
Vivo4
  • Comparative assessment of fluorescent proteins for in vivo imaging in an animal model system. (cam.ac.uk)
  • To address the gap, we quantitatively assessed fluorescent protein properties in vivo in an animal model system. (cam.ac.uk)
  • The Caenorhabditis elegans hermaphrodite germ line provides a simple and accessible system for studying stem cells in vivo In this system, GLP-1/Notch activity prevents the differentiation of distal germ cells in response to ligand production from the nearby distal tip cell, thereby supporting a stem cell pool. (bvsalud.org)
  • IMSEAR at SEARO: Development and evaluation of an in vivo assay in Caenorhabditis elegans for screening of compounds for their effect on cytochrome P450 expression. (who.int)
Transmembrane protein1
  • Fas ligand is a transmembrane protein part of the tumor necrosis factor (TNF) family. (smpdb.ca)
Model4
  • Consistent with previous data, we documented a clear inverse correlation between antimicrobial resistance and virulence in the C. elegans model. (repositoriosaludmadrid.es)
  • The highest virulence of ST235 could be attributed to its exoU+ type III secretion system (TTSS) genotype, which was found to be linked with higher virulence in our C. elegans model. (repositoriosaludmadrid.es)
  • Other markers, such as motility or pigment production, were not essential for virulence in the C. elegans model but seemed to be related with the higher values of the statistical normalized data. (repositoriosaludmadrid.es)
  • In contrast to ST235, the ST175 high-risk clone, which is widespread in Spain and France, seems to be associated with a particularly low virulence in the C. elegans model. (repositoriosaludmadrid.es)
Development6
  • Together, this work improves our use and understanding of the AID system for dissecting gene function at the single-cell level during C. elegans development. (nih.gov)
  • C. elegans deficient in Moco also die very early in development. (genengnews.com)
  • I had originally intended to ask him about Professor Frederick Sanger, the two-time Nobel Prize winner famous for his discovery of the structure of proteins and his development of DNA sequencing methods, who passed away in November. (elizabethdzeng.com)
  • In this work, azidohomoalanine (AHA)-specific labeling was used to identify proteins expressed by E . granulosus protoscoleces (PSCs) upon the induction of strobilar development. (cdc.gov)
  • After the controlled-labeling of proteins during the induction of strobilar development, we identified modifications in protein expression. (cdc.gov)
  • We used different protein tags that allowed for the visualization and purification of proteins produced specifically after the induction of strobilar development to identify proteins that might be involved in this process (temporally controlled and context-dependent). (cdc.gov)
Domains3
  • However, even CRISPR alleles encoding heritable fluorescently-tagged protein markers can result in defects in function or localization of the gene product if the fluorescent tag obstructs or otherwise interferes with important protein interaction domains or affects the protein structure. (bio-protocol.org)
  • There are 9609 ADF domains in 8060 proteins in SMART's nrdb database. (embl.de)
  • Until now, proteins containing both LOTUS and Tudor domains in Caenorhabditis elegans have remained elusive. (bvsalud.org)
Defects1
  • 2005 ) Regulatory mutations of mir-48, a C. elegans let-7 family MicroRNA, cause developmental timing defects. (neurotree.org)
Genetic2
  • This abnormal construction has been linked to genetic mutations in a family of proteins called collapsin response mediator proteins (CRMPs). (truman.edu)
  • ED: Today, the structure of DNA and how genetic information is translated into proteins are established scientific canon, but in the 1950s, the hypotheses generated at the LMB were dismissed as inconceivable nonsense. (elizabethdzeng.com)
Interact1
  • The aryl hydrocarbon receptor (AhR) has been shown to interact with an immunophilin-like molecule known as AhR-interacting protein (AIP) and to enhance AhR function. (cdc.gov)
Expression2
  • GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. (genscript.com)
  • The estrogen receptor is a protein that binds to DNA to increase (activate) or decrease (repress) gene expression. (blogspot.com)
IUCr) Acta Crystallographica Section D Volume 60, Part 10, October 2004
IUCr) Acta Crystallographica Section D Volume 60, Part 10, October 2004 (journals.iucr.org)
Biblio | Journal of Alzheimer's Disease
Biblio | Journal of Alzheimer's Disease (j-alz.com)
Kindler epidermolysis bullosa: MedlinePlus Genetics
Kindler epidermolysis bullosa: MedlinePlus Genetics (medlineplus.gov)
FERMT1 gene: MedlinePlus Genetics
FERMT1 gene: MedlinePlus Genetics (medlineplus.gov)
Positive regulation of hypodermal cell differentiation | Semantic Scholar
Positive regulation of hypodermal cell differentiation | Semantic Scholar (semanticscholar.org)
Category:Chromatin - Wikimedia Commons
Category:Chromatin - Wikimedia Commons (commons.wikimedia.org)
The SensorOverlord predicts the accuracy of measurements with ratiometric biosensors | Scientific Reports
The SensorOverlord predicts the accuracy of measurements with ratiometric biosensors | Scientific Reports (nature.com)
Sol Genomics Network
Sol Genomics Network (sgn.cornell.edu)
Thomas M. Roberts, PhD - Dana-Farber Cancer Institute | Boston, MA
Thomas M. Roberts, PhD - Dana-Farber Cancer Institute | Boston, MA (dana-farber.org)
Spp-12 gene cDNA ORF clone, Caenorhabditis elegans - GenScript
Spp-12 gene cDNA ORF clone, Caenorhabditis elegans - GenScript (genscript.com)
Anti-6X His tag® antibody [4D11] (ab5000) | Abcam
Anti-6X His tag® antibody [4D11] (ab5000) | Abcam (abcam.com)
Comparative genomic analysis of innate immunity reveals novel and conserved components in crustacean food crop species | BMC...
Comparative genomic analysis of innate immunity reveals novel and conserved components in crustacean food crop species | BMC... (bmcgenomics.biomedcentral.com)
Vitamin E Does Not Cause Prostate Cancer | Baseline of Health Foundation
Vitamin E Does Not Cause Prostate Cancer | Baseline of Health Foundation (jonbarron.org)
Tim Stearns' Profile | Stanford Profiles
Tim Stearns' Profile | Stanford Profiles (profiles.stanford.edu)
Ein Protein als Katastrophenhelfer - TUM
Ein Protein als Katastrophenhelfer - TUM (tum.de)
Simple model systems: a challenge for Alzheimer's disease | SpringerLink
Simple model systems: a challenge for Alzheimer's disease | SpringerLink (link.springer.com)
Germline stem cells in human | Signal Transduction and Targeted Therapy
Germline stem cells in human | Signal Transduction and Targeted Therapy (nature.com)
SMART: CAP_GLY domain annotation
SMART: CAP_GLY domain annotation (smart.embl.de)
SMART: RGS domain annotation
SMART: RGS domain annotation (smart.embl.de)
Critical Residues of the Caenorhabditis elegans unc-2 Voltage-Gated Calcium Channel That Affect Behavioral and Physiological...
Critical Residues of the Caenorhabditis elegans unc-2 Voltage-Gated Calcium Channel That Affect Behavioral and Physiological... (jneurosci.org)
Katherine Fitzgerald | Profiles RNS
Katherine Fitzgerald | Profiles RNS (profiles.umassmed.edu)
John-Olov Jansson,
John-Olov Jansson, (gu.se)
SMART: Schnipsel domain WD40
SMART: Schnipsel domain WD40 (smart.embl.de)