Cadherins. Medical search

Calcium-dependent cell adhesion proteins. They are important in the formation of ADHERENS JUNCTIONS between cells. Cadherins are classified by their distinct immunological and tissue specificities, either by letters (E- for epithelial, N- for neural, and P- for placental cadherins) or by numbers (cadherin-12 or N-cadherin 2 for brain-cadherin). Cadherins promote cell adhesion via a homophilic mechanism as in the construction of tissues and of the whole animal body.

A single-pass transmembrane glycoproteins that mediate CALCIUM-dependent CELL ADHESION and are core components of DESMOSOMES.

A group of desmosomal cadherins with cytoplasmic tails that resemble those of classical CADHERINS.

Desmoplakins are cytoskeletal linker proteins that anchor INTERMEDIATE FILAMENTS to the PLASMA MEMBRANE at DESMOSOMES.

A group of desmosomal cadherins with cytoplasmic tails that are divergent from those of classical CADHERINS. Their intracytoplasmic domains bind PLAKOGLOBIN; PLAKOPHILINS; and DESMOPLAKINS.

A family of cytoskeletal proteins that play essential roles in CELL ADHESION at ADHERENS JUNCTIONS by linking CADHERINS to the ACTIN FILAMENTS of the CYTOSKELETON.

A multi-functional catenin that is highly homologous to BETA CATENIN. Gamma catenin binds CADHERINS and helps link their cytoplasmic tails to ACTIN in the CYTOSKELETON via ALPHA CATENIN. It is also found in DESMOSOMES where it mediates the link between DESMOSOMAL CADHERINS and DESMOPLAKIN.

A type of junction that attaches one cell to its neighbor. One of a number of differentiated regions which occur, for example, where the cytoplasmic membranes of adjacent epithelial cells are closely apposed. It consists of a circular region of each membrane together with associated intracellular microfilaments and an intercellular material which may include, for example, mucopolysaccharides. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990; Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)

A catenin that binds F-ACTIN and links the CYTOSKELETON with BETA CATENIN and GAMMA CATENIN.

A CALCIUM-dependent adhesion molecule of DESMOSOMES that also plays a role in embryonic STEM CELL proliferation.

Adherence of cells to surfaces or to other cells.

Major constituent of the cytoskeleton found in the cytoplasm of eukaryotic cells. They form a flexible framework for the cell, provide attachment points for organelles and formed bodies, and make communication between parts of the cell possible.

A desmosomal cadherin that is an autoantigen in the acquired skin disorder PEMPHIGUS FOLIACEUS.

Direct contact of a cell with a neighboring cell. Most such junctions are too small to be resolved by light microscopy, but they can be visualized by conventional or freeze-fracture electron microscopy, both of which show that the interacting CELL MEMBRANE and often the underlying CYTOPLASM and the intervening EXTRACELLULAR SPACE are highly specialized in these regions. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p792)

A multi-functional catenin that participates in CELL ADHESION and nuclear signaling. Beta catenin binds CADHERINS and helps link their cytoplasmic tails to the ACTIN in the CYTOSKELETON via ALPHA CATENIN. It also serves as a transcriptional co-activator and downstream component of WNT PROTEIN-mediated SIGNAL TRANSDUCTION PATHWAYS.

Anchoring points where the CYTOSKELETON of neighboring cells are connected to each other. They are composed of specialized areas of the plasma membrane where bundles of the ACTIN CYTOSKELETON attach to the membrane through the transmembrane linkers, CADHERINS, which in turn attach through their extracellular domains to cadherins in the neighboring cell membranes. In sheets of cells, they form into adhesion belts (zonula adherens) that go all the way around a cell.

Surface ligands, usually glycoproteins, that mediate cell-to-cell adhesion. Their functions include the assembly and interconnection of various vertebrate systems, as well as maintenance of tissue integration, wound healing, morphogenic movements, cellular migrations, and metastasis.

The phenomenon by which dissociated cells intermixed in vitro tend to group themselves with cells of their own type.

A desmosomal cadherin that is an autoantigen in the acquired skin disorder PEMPHIGUS VULGARIS.

A family of proteins that contain several 42-amino acid repeat domains and are homologous to the Drosophila armadillo protein. They bind to other proteins through their armadillo domains and play a variety of roles in the CELL including SIGNAL TRANSDUCTION, regulation of DESMOSOME assembly, and CELL ADHESION.

Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.

The development of anatomical structures to create the form of a single- or multi-cell organism. Morphogenesis provides form changes of a part, parts, or the whole organism.

A cultured line of C3H mouse FIBROBLASTS that do not adhere to one another and do not express CADHERINS.

Members of the armadillo family of proteins that are found in DESMOSOMES and interact with various proteins including desmocadherins; DESMOPLAKIN; ACTIN FILAMENTS; and KERATINS.

The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

Any of several ways in which living cells of an organism communicate with one another, whether by direct contact between cells or by means of chemical signals carried by neurotransmitter substances, hormones, and cyclic AMP.

The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.

The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.

Group of chronic blistering diseases characterized histologically by ACANTHOLYSIS and blister formation within the EPIDERMIS.

A subclass of receptor-like protein tryosine phosphatases that contain multiple extracellular immunoglobulin G-like domains and fibronectin type III-like domains. An additional memprin-A5-mu domain is found on some members of this subclass.

The network of filaments, tubules, and interconnecting filamentous bridges which give shape, structure, and organization to the cytoplasm.

Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action during the developmental stages of an organism.

Proteins obtained from various species of Xenopus. Included here are proteins from the African clawed frog (XENOPUS LAEVIS). Many of these proteins have been the subject of scientific investigations in the area of MORPHOGENESIS and development.

A cytoskeletal protein associated with cell-cell and cell-matrix interactions. The amino acid sequence of human vinculin has been determined. The protein consists of 1066 amino acid residues and its gene has been assigned to chromosome 10.

A subclass of receptor-like protein tryosine phosphatases that contain an extracellular RDGS-adhesion recognition motif and a single cytosolic protein tyrosine phosphate domain.

Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.

Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.

Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.

Orientation of intracellular structures especially with respect to the apical and basolateral domains of the plasma membrane. Polarized cells must direct proteins from the Golgi apparatus to the appropriate domain since tight junctions prevent proteins from diffusing between the two domains.

Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.

Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.

Specialized areas at the CELL MEMBRANE where a cell attaches to the EXTRACELLULAR MATRIX or other substratum.

A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.

The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.

Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.

Established cell cultures that have the potential to propagate indefinitely.

The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.

Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate.

Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.

The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.

Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.

A family of transmembrane glycoproteins (MEMBRANE GLYCOPROTEINS) consisting of noncovalent heterodimers. They interact with a wide variety of ligands including EXTRACELLULAR MATRIX PROTEINS; COMPLEMENT, and other cells, while their intracellular domains interact with the CYTOSKELETON. The integrins consist of at least three identified families: the cytoadhesin receptors(RECEPTORS, CYTOADHESIN), the leukocyte adhesion receptors (RECEPTORS, LEUKOCYTE ADHESION), and the VERY LATE ANTIGEN RECEPTORS. Each family contains a common beta-subunit (INTEGRIN BETA CHAINS) combined with one or more distinct alpha-subunits (INTEGRIN ALPHA CHAINS). These receptors participate in cell-matrix and cell-cell adhesion in many physiologically important processes, including embryological development; HEMOSTASIS; THROMBOSIS; WOUND HEALING; immune and nonimmune defense mechanisms; and oncogenic transformation.

The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.

A rac GTP-binding protein involved in regulating actin filaments at the plasma membrane. It controls the development of filopodia and lamellipodia in cells and thereby influences cellular motility and adhesion. It is also involved in activation of NADPH OXIDASE. This enzyme was formerly listed as EC 3.6.1.47.

The external, nonvascular layer of the skin. It is made up, from within outward, of five layers of EPITHELIUM: (1) basal layer (stratum basale epidermidis); (2) spinous layer (stratum spinosum epidermidis); (3) granular layer (stratum granulosum epidermidis); (4) clear layer (stratum lucidum epidermidis); and (5) horny layer (stratum corneum epidermidis).

Separation of the prickle cells of the stratum spinosum of the epidermis, resulting in atrophy of the prickle cell layer. It is seen in diseases such as pemphigus vulgaris (see PEMPHIGUS) and DARIER DISEASE.

A process of complicated morphogenetic cell movements that reorganizes a bilayer embryo into one with three GERM LAYERS and specific orientation (dorsal/ventral; anterior/posterior). Gastrulation describes the germ layer development of a non-mammalian BLASTULA or that of a mammalian BLASTOCYST.

The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)

A sequential pattern of amino acids occurring more than once in the same protein sequence.

Microscopy in which the samples are first stained immunocytochemically and then examined using an electron microscope. Immunoelectron microscopy is used extensively in diagnostic virology as part of very sensitive immunoassays.

Histochemical localization of immunoreactive substances using labeled antibodies as reagents.

Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.

The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.

The process by which cells convert mechanical stimuli into a chemical response. It can occur in both cells specialized for sensing mechanical cues such as MECHANORECEPTORS, and in parenchymal cells whose primary function is not mechanosensory.

A MARVEL domain-containing protein found in the presynaptic vesicles of NEURONS and NEUROENDOCRINE CELLS. It is commonly used as an immunocytochemical marker for neuroendocrine differentiation.

Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.

The process by which two molecules of the same chemical composition form a condensation product or polymer.

A large family of MONOMERIC GTP-BINDING PROTEINS that are involved in regulation of actin organization, gene expression and cell cycle progression. This enzyme was formerly listed as EC 3.6.1.47.

A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.

Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.

The commonest and widest ranging species of the clawed "frog" (Xenopus) in Africa. This species is used extensively in research. There is now a significant population in California derived from escaped laboratory animals.

The parts of a macromolecule that directly participate in its specific combination with another molecule.

Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.

Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.

The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.

The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.

The developmental entity of a fertilized egg (ZYGOTE) in animal species other than MAMMALS. For chickens, use CHICK EMBRYO.

A member of the Rho family of MONOMERIC GTP-BINDING PROTEINS. It is associated with a diverse array of cellular functions including cytoskeletal changes, filopodia formation and transport through the GOLGI APPARATUS. This enzyme was formerly listed as EC 3.6.1.47.

CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.

Cytoplasmic filaments intermediate in diameter (about 10 nanometers) between the microfilaments and the microtubules. They may be composed of any of a number of different proteins and form a ring around the cell nucleus.

The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.

Explanations for the clinical and microscopic localization of lesions in pemphigus foliaceus and vulgaris. (1/6757)

Patients with pemphigus foliaceus (PF) have blisters on skin, but not mucous membranes, whereas patients with pemphigus vulgaris (PV) develop blisters on mucous membranes and/or skin. PF and PV blisters are due to loss of keratinocyte cell-cell adhesion in the superficial and deep epidermis, respectively. PF autoantibodies are directed against desmoglein (Dsg) 1; PV autoantibodies bind Dsg3 or both Dsg3 and Dsg1. In this study, we test the hypothesis that coexpression of Dsg1 and Dsg3 in keratinocytes protects against pathology due to antibody-induced dysfunction of either one alone. Using passive transfer of pemphigus IgG to normal and DSG3(null) neonatal mice, we show that in the areas of epidermis and mucous membrane that coexpress Dsg1 and Dsg3, antibodies against either desmoglein alone do not cause spontaneous blisters, but antibodies against both do. In areas (such as superficial epidermis of normal mice) where Dsg1 without Dsg3 is expressed, anti-Dsg1 antibodies alone can cause blisters. Thus, the anti-desmoglein antibody profiles in pemphigus sera and the normal tissue distributions of Dsg1 and Dsg3 determine the sites of blister formation. These studies suggest that pemphigus autoantibodies inhibit the adhesive function of desmoglein proteins, and demonstrate that either Dsg1 or Dsg3 alone is sufficient to maintain keratinocyte adhesion. (+info)

p27 is involved in N-cadherin-mediated contact inhibition of cell growth and S-phase entry. (2/6757)

In this study the direct involvement of cadherins in adhesion-mediated growth inhibition was investigated. It is shown here that overexpression of N-cadherin in CHO cells significantly suppresses their growth rate. Interaction of these cells and two additional fibroblastic lines with synthetic beads coated with N-cadherin ligands (recombinant N-cadherin ectodomain or specific antibodies) leads to growth arrest at the G1 phase of the cell cycle. The cadherin-reactive beads inhibit the entry into S phase and the reduction in the levels of cyclin-dependent kinase (cdk) inhibitors p21 and p27, following serum-stimulation of starved cells. In exponentially growing cells these beads induce G1 arrest accompanied by elevation in p27 only. We propose that cadherin-mediated signaling is involved in contact inhibition of growth by inducing cell cycle arrest at the G1 phase and elevation of p27 levels. (+info)

Tracing cellular and molecular mechanisms involved in endometriosis. (3/6757)

The aetiology and pathogenesis of endometriosis, defined as the presence of endometrium-like tissue outside the uterine cavity, is largely unknown. In this paper we present and discuss possibilities to study the putative pathogenic properties of endometriotic cells in vitro. The current focus of our investigations is on the invasive phenotype of the disease, assuming that this might contribute to the pathogenesis of endometriosis. So far, we have shown that: (i) cytokeratin-positive and E-cadherin-negative endometriotic cells have an invasive phenotype in a collagen invasion assay in vitro similar to metastatic carcinoma cells; (ii) the invasiveness of endometriotic but not of eutopic endometrial cells can be stimulated by a heat-stable protein present in peritoneal fluid; and (iii) the endometriotic cell line EEC145T, which we established, may be a useful tool for the identification of gene products which are, positively or negatively, invasion-related. Finally, our studies suggest that the invasive phenotype in endometriosis shares aspects with tumour metastasis, but might also have unique mechanisms. (+info)

Cadherin-11 is expressed in invasive breast cancer cell lines. (4/6757)

In several cancers, including breast cancer, loss of E-cadherin expression is correlated with a loss of the epithelial phenotype and with a gain of invasiveness. Cells that have lost E-cadherin expression are either poorly invasive with a rounded phenotype, or highly invasive, with a mesenchymal phenotype. Most cells lacking E-cadherin still retain weak calcium-dependent adhesion, indicating the presence of another cadherin family member. We have now examined the expression of the mesenchymal cadherin, cadherin-11, in breast cancer cell lines. Cadherin-11 mRNA and protein, as well as a variant form, are expressed in the most invasive cell lines but not in any of the noninvasive cell lines. Cadherin-11 is localized to a detergent-soluble pool and is associated with both alpha- and beta-catenin. Immunocytochemistry shows that cadherin-11 is localized to the cell membrane at sites of cell-cell contact as well as at lamellipodia-like projections, which do not interact with other cells. These results suggest that cadherin-11 expression may be well correlated with the invasive phenotype in cancer cells and may serve as a molecular marker for the more aggressive, invasive subset of tumors. Cadherin-11 may mediate the interaction between malignant tumor cells and other cell types that normally express cadherin-11, such as stromal cells or osteoblasts or perhaps even with the surrounding extracellular matrix, thus facilitating tumor cell invasion and metastasis. (+info)

Coupling assembly of the E-cadherin/beta-catenin complex to efficient endoplasmic reticulum exit and basal-lateral membrane targeting of E-cadherin in polarized MDCK cells. (5/6757)

The E-cadherin/catenin complex regulates Ca++-dependent cell-cell adhesion and is localized to the basal-lateral membrane of polarized epithelial cells. Little is known about mechanisms of complex assembly or intracellular trafficking, or how these processes might ultimately regulate adhesion functions of the complex at the cell surface. The cytoplasmic domain of E-cadherin contains two putative basal-lateral sorting motifs, which are homologous to sorting signals in the low density lipoprotein receptor, but an alanine scan across tyrosine residues in these motifs did not affect the fidelity of newly synthesized E-cadherin delivery to the basal-lateral membrane of MDCK cells. Nevertheless, sorting signals are located in the cytoplasmic domain since a chimeric protein (GP2CAD1), comprising the extracellular domain of GP2 (an apical membrane protein) and the transmembrane and cytoplasmic domains of E-cadherin, was efficiently and specifically delivered to the basal-lateral membrane. Systematic deletion and recombination of specific regions of the cytoplasmic domain of GP2CAD1 resulted in delivery of <10% of these newly synthesized proteins to both apical and basal-lateral membrane domains. Significantly, >90% of each mutant protein was retained in the ER. None of these mutants formed a strong interaction with beta-catenin, which normally occurs shortly after E-cadherin synthesis. In addition, a simple deletion mutation of E-cadherin that lacks beta-catenin binding is also localized intracellularly. Thus, beta-catenin binding to the whole cytoplasmic domain of E-cadherin correlates with efficient and targeted delivery of E-cadherin to the lateral plasma membrane. In this capacity, we suggest that beta-catenin acts as a chauffeur, to facilitate transport of E-cadherin out of the ER and the plasma membrane. (+info)

Mutated epithelial cadherin is associated with increased tumorigenicity and loss of adhesion and of responsiveness to the motogenic trefoil factor 2 in colon carcinoma cells. (6/6757)

Epithelial (E)-cadherin and its associated cytoplasmic proteins (alpha-, beta-, and gamma-catenins) are important mediators of epithelial cell-cell adhesion and intracellular signaling. Much evidence exists suggesting a tumor/invasion suppressor role for E-cadherin, and loss of expression, as well as mutations, has been described in a number of epithelial cancers. To investigate whether E-cadherin gene (CDH1) mutations occur in colorectal cancer, we screened 49 human colon carcinoma cell lines from 43 patients by single-strand conformation polymorphism (SSCP) analysis and direct sequencing. In addition to silent changes, polymorphisms, and intronic variants in a number of the cell lines, we detected frameshift single-base deletions in repeat regions of exon 3 (codons 120 and 126) causing premature truncations at codon 216 in four replication-error-positive (RER+) cell lines (LS174T, HCT116, GP2d, and GP5d) derived from 3 patients. In LS174T such a mutation inevitably contributes to its lack of E-cadherin protein expression and function. Transfection of full-length E-cadherin cDNA into LS174T cells enhanced intercellular adhesion, induced differentiation, retarded proliferation, inhibited tumorigenicity, and restored responsiveness to the migratory effects induced by the motogenic trefoil factor 2 (human spasmolytic polypeptide). These results indicate that, although inactivating E-cadherin mutations occur relatively infrequently in colorectal cancer cell lines overall (3/43 = 7%), they are more common in cells with an RER+ phenotype (3/10 = 30%) and may contribute to the dysfunction of the E-cadherin-catenin-mediated adhesion/signaling system commonly seen in these tumors. These results also indicate that normal E-cadherin-mediated cell adhesion can restore the ability of colonic tumor cells to respond to trefoil factor 2. (+info)

Misexpression of the catenin p120(ctn)1A perturbs Xenopus gastrulation but does not elicit Wnt-directed axis specification. (7/6757)

Modulators of cadherin function are of great interest given that the cadherin complex actively contributes to the morphogenesis of virtually all tissues. The catenin p120(ctn) (formerly p120cas) was first identified as a src- and receptor-protein tyrosine kinase substrate and later shown to interact directly with cadherins. In common with beta-catenin and plakoglobin (gamma-catenin), p120(ctn) contains a central Armadillo repeat region by which it binds cadherin cytoplasmic domains. However, little is known about the function of p120(ctn) within the cadherin complex. We examined the role of p120(ctn)1A in early vertebrate development via its exogenous expression in Xenopus. Ventral overexpression of p120(ctn)1A, in contrast to beta-catenin, did not induce the formation of duplicate axial structures resulting from the activation of the Wnt signaling pathway, nor did p120(ctn) affect mesoderm induction. Rather, dorsal misexpression of p120(ctn) specifically perturbed gastrulation. Lineage tracing of cells expressing exogenous p120(ctn) indicated that cell movements were disrupted, while in vitro studies suggested that this may have been a consequence of reduced adhesion between blastomeres. Thus, while cadherin-binding proteins beta-catenin, plakoglobin, and p120(ctn) are members of the Armadillo protein family, it is clear that these proteins have distinct biological functions in early vertebrate development. This work indicates that p120(ctn) has a role in cadherin function and that heightened expression of p120(ctn) interferes with appropriate cell-cell interactions necessary for morphogenesis. (+info)

The expression of beta-catenin in non-small-cell lung cancer: a clinicopathological study. (8/6757)

AIMS: To investigate the expression of beta-catenin in non-small-cell lung cancer (NSCLC) and its clinical significance. METHODS: 101 patients were surgically treated for NSCLC by lobectomy or pneumectomy with systematic lymph node dissection. Follow up was available in all patients, ranging from 24 to 110 months. Immunostaining of tissue sections from primary tumours and (when present) their lymph node metastases was performed and evaluated using a monoclonal antibody against beta-catenin. Correlations were investigated between beta-catenin immunostaining in primary tumours and E-cadherin immunostaining (data available from a previous study), lymph node stage, and survival. RESULTS: There were significant correlations between scores for beta-catenin immunostaining and E-cadherin immunostaining in primary tumours (p = 0.007), and between the beta-catenin immunostaining score in primary tumours and in their lymph node metastases (p = 0.006). An inverse correlation was found between the beta-catenin immunostaining score in primary tumours and lymph node stage N0, N1, or N2 (p = 0.03). According to the Kaplan-Meier survival estimate, the level of beta-catenin expression in primary tumours was a statistically significant prognostic factor (p = 0.01). CONCLUSIONS: Reduced beta-catenin expression in surgically treated NSCLC is clearly associated with lymph node metastasis and an infavourable prognosis. The existence of a functional relation between E-cadherin and beta-catenin is supported by the results of this clinicopathological study. (+info)

2000). "Clustered cadherin genes: a sequence-ready contig for the desmosomal cadherin locus on human chromosome 18". Genomics. ... Garrod DR, Merritt AJ, Nie Z (2003). "Desmosomal cadherins". Curr. Opin. Cell Biol. 14 (5): 537-45. doi:10.1016/S0955-0674(02) ... Jun 1993). "Nomenclature of the desmosomal cadherins". J Cell Biol. 121 (3): 481-3. doi:10.1083/jcb.121.3.481. PMC 2119574. ... 2000). "Genomic organization and amplification of the human desmosomal cadherin genes DSC1 and DSC3, encoding desmocollin types ...

https://en.wikipedia.org/wiki/DSC1

... is required for its high affinity binding to the cytoplasmic domains of E-cadherin and desmosomal cadherin Dsg2, and the tumor ... is required for its high affinity binding to the cytoplasmic domains of E-cadherin and desmosomal cadherin Dsg2, and the tumor ... Bannon LJ, Cabrera BL, Stack MS, Green KJ (Nov 2001). "Isoform-specific differences in the size of desmosomal cadherin/catenin ... Bannon LJ, Cabrera BL, Stack MS, Green KJ (Nov 2001). "Isoform-specific differences in the size of desmosomal cadherin/catenin ...

https://en.wikipedia.org/wiki/Desmoglein-2

They are involved in regulating the adhesive activity of cadherin. The three types of plakophilin proteins found in humans are ... The Molecular Biology of Cadherins. Academic Press. 116: 387-407. doi:10.1016/b978-0-12-394311-8.00017-0. PMC 3752792. PMID ...

https://en.wikipedia.org/wiki/Plakophilin

Desmosomal cadherins, including the desmocollin family members and desmogleins, are found at desmosome cell-cell junctions and ... Cadherin-like junctional molecules generated by alternative splicing". The Journal of Biological Chemistry. 266 (16): 10438-45 ... Desmocollin-2 is a cadherin-type protein that functions to link adjacent cells together in specialized regions known as ... Desmocollin-2 is a calcium-dependent glycoprotein that is a member of the desmocollin subfamily of the cadherin superfamily. ...

https://en.wikipedia.org/wiki/DSC2

Rolf Kemler's anti serum was what helped him finally identify cadherin. E-cadherin was the first of the cadherin family to be ... After his discovery of cadherins, he continued to research cadherins and their properties. Along with cadherins, he also ... An assay showed the hemaglutinin interaction is specific to e-cadherin. Outside of cadherin, Takeichi studied other molecules ... They found the hemagglutinin directly interacted with the e-cadherin in the epithelial cells to disturbed cell to cell adhesion ...

https://en.wikipedia.org/wiki/Masatoshi_Takeichi

Desmocollin-3 is a calcium-dependent glycoprotein that is a member of the desmocollin subfamily of the cadherin superfamily. ... Jun 1993). "Nomenclature of the desmosomal cadherins" (PDF). J Cell Biol. 121 (3): 481-3. doi:10.1083/jcb.121.3.481. PMC ... 2001). "Down-regulation of the desmosomal cadherin desmocollin 3 in human breast cancer". Int. J. Oncol. 19 (1): 169-74. doi: ... 2000). "Genomic organization and amplification of the human desmosomal cadherin genes DSC1 and DSC3, encoding desmocollin types ...

https://en.wikipedia.org/wiki/DSC3

Müller, U (October 2008). "Cadherins and mechanotransduction by hair cells". Current Opinion in Cell Biology. 20 (5): 557-566. ...

https://en.wikipedia.org/wiki/Hair_cell

Cadherins are calcium-dependent adhesion molecules. Cadherins are extremely important in the process of morphogenesis - fetal ... Cadherin molecules form the actual anchor by attaching to the cytoplasmic plaque, extending through the membrane and binding ... Together with an alpha-beta catenin complex, the cadherin can bind to the microfilaments of the cytoskeleton of the cell. This ... Similarly to desmosomes and hemidesmosomes, their transmembrane anchors are composed of cadherins in those that anchor to other ...

https://en.wikipedia.org/wiki/Cell_junction

Amagai M (1999). "Autoimmunity against desmosomal cadherins in pemphigus". J. Dermatol. Sci. 20 (2): 92-102. doi:10.1016/S0923- ...

https://en.wikipedia.org/wiki/Periplakin

Cadherin-17 is a protein that in humans is encoded by the CDH17 gene. This gene is a member of the cadherin superfamily, genes ... 2004). "Ksp-cadherin is a functional cell-cell adhesion molecule related to LI-cadherin". Exp. Cell Res. 294 (2): 345-55. doi: ... "Entrez Gene: CDH17 cadherin 17, LI cadherin (liver-intestine)". Gessner R, Tauber R (2001). "Intestinal cell adhesion molecules ... The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane ...

https://en.wikipedia.org/wiki/CDH17

The encoded protein has a signal peptide, 27 cadherin repeat domains and a unique cytoplasmic region. This particular cadherin ... Protein dachsous homolog 1, also known as protocadherin-16 (PCDH16) or cadherin-19 (CDH19) or cadherin-25 (CDH25) or fibroblast ... cadherin-1 (FIB1), is a protein that in humans is encoded by the DCHS1 gene. This gene is a member of the cadherin superfamily ... 2001). "Identification of three novel non-classical cadherin genes through comprehensive analysis of large cDNAs". Brain Res. ...

https://en.wikipedia.org/wiki/DCHS1

... forms distinct complexes with cadherins and desmosomal cadherins. Plakoglobin is a major cytoplasmic component of ... is required for its high affinity binding to the cytoplasmic domains of E-cadherin and desmosomal cadherin Dsg2, and the tumor ... interacts with E-cadherin and N-cadherin". The Journal of Cell Biology. 118 (3): 671-9. doi:10.1083/jcb.118.3.671. PMC 2289540 ... interacts with E-cadherin and N-cadherin". The Journal of Cell Biology. 118 (3): 671-9. doi:10.1083/jcb.118.3.671. PMC 2289540 ...

https://en.wikipedia.org/wiki/Plakoglobin

P-cadherins), neural (N-cadherins), retinal (R-cadherins), brain (B-cadherins and T-cadherins), and muscle (M-cadherins). Many ... The cadherins are homophilic Ca2+ -dependent glycoproteins. The classic cadherins (E-, N- and P-) are concentrated at the ... Cadherins are notable in embryonic development. For example, cadherins are crucial in gastrulation for the formation of the ... The diverse family of cadherins include epithelial (E-cadherins), placental ( ...

https://en.wikipedia.org/wiki/Cell_adhesion_molecule

These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of ... Wu Q, Maniatis T (Jul 1999). "A striking organization of a large family of human neural cadherin-like cell adhesion genes". ... Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. ... Matsuyoshi N, Imamura S (1997). "Multiple cadherins are expressed in human fibroblasts". Biochem. Biophys. Res. Commun. 235 (2 ...

https://en.wikipedia.org/wiki/PCDHGA12

Larue, L.; Antos, C.; Butz, S.; Huber, O.; Delmas, V.; Dominis, M.; Kemler, R. (1996). "A role for cadherins in tissue ... Burdsal, C. A.; Damsky, C. H.; Pedersen, R. A. (1993). "The role of E-cadherin and integrins in mesoderm differentiation and ... EBs are formed by the homophilic binding of the Ca2+ dependent adhesion molecule E-cadherin, which is highly expressed on ...

https://en.wikipedia.org/wiki/Embryoid_body

The human FAT1 cadherin gene was cloned in 1995 from a human T-leukemia (T-ALL) cell line and consists of 27 exons located on ... FAT1 cadherin is multiply phosphorylated on its ectodomain but phosphorylation is not catalysed by FJX1. The ectodomain of FAT1 ... The FAT1 cadherin has been ascribed both as putative tumour suppressor or oncogene in different contexts. Loss of ... December 2015). "FAT1 cadherin acts upstream of Hippo signalling through TAZ to regulate neuronal differentiation". Cellular ...

https://en.wikipedia.org/wiki/FAT1

Cadherin 10 is a protein that in humans is encoded by the CDH10 gene. An association with autism has been suggested. Cadherin ... "Entrez Gene: cadherin 10". Suzuki S, Sano K, Tanihara H (April 1991). "Diversity of the cadherin family: evidence for eight new ... Ali J, Liao F, Martens E, Muller WA (1997). "Vascular endothelial cadherin (VE-cadherin): cloning and role in endothelial cell- ... Kools P, Vanhalst K, Van den Eynde E, van Roy F (1999). "The human cadherin-10 gene: complete coding sequence, predominant ...

https://en.wikipedia.org/wiki/CDH10

Cadherin 9 is a protein that in humans is encoded by the CDH9 gene. An association with autism has been suggested. Cadherin ... "Entrez Gene: cadherin 9". Suzuki S, Sano K, Tanihara H (April 1991). "Diversity of the cadherin family: evidence for eight new ... Ali J, Liao F, Martens E, Muller WA (June 1997). "Vascular endothelial cadherin (VE-cadherin): cloning and role in endothelial ... Thedieck C, Kalbacher H, Kuczyk M, Müller GA, Müller CA, Klein G (2007). Zoccali C (ed.). "Cadherin-9 is a novel cell surface ...

https://en.wikipedia.org/wiki/CDH9

Dusek, Rachel L; Godsel, Lisa M.; l, Kathleen J. (January 2007). "Discriminating roles of desmosomal cadherins:Beyond ...

https://en.wikipedia.org/wiki/Kathleen_J._Green

Cadherin-1), CDH2 N-cadherin (Cadherin-2), CDH4 R-cadherin (cadherin-4), CDH5 VE-cadherin (cadherin 5, CDH5), CTNND1 ( ... Cadherins regulate cell-cell adhesion during development of the body and in adult tissue. Disruption of cadherin proteins, by ... Cadherins stabilize adherens junctions through the interaction of the cadherin cytoplasmic domains with catenin proteins, such ... PTPmu likely regulates cadherin-dependent adhesion by interacting with both cadherins and catenins via PTPmu's cytoplasmic ...

https://en.wikipedia.org/wiki/PTPRM

The flamingo cadherins are located at the plasma membrane and have nine cadherin domains, seven epidermal growth factor-like ... Cadherin EGF LAG seven-pass G-type receptor 2 is a protein that in humans is encoded by the CELSR2 gene. The protein encoded by ... "Entrez Gene: CELSR2 cadherin, EGF LAG seven-pass G-type receptor 2 (flamingo homolog, Drosophila)". Nagase T, Seki N, Ishikawa ... Wu Q, Maniatis T (1999). "A striking organization of a large family of human neural cadherin-like cell adhesion genes". Cell. ...

https://en.wikipedia.org/wiki/CELSR2

The flamingo cadherins are located at the plasma membrane and have nine cadherin domains, seven epidermal growth factor-like ... Cadherin EGF LAG seven-pass G-type receptor 3 is a protein that in humans is encoded by the CELSR3 gene. The protein encoded by ... "Entrez Gene: CELSR3 cadherin, EGF LAG seven-pass G-type receptor 3 (flamingo homolog, Drosophila)". Bonaldo MF, Lennon G, ... Wu Q, Maniatis T (1999). "A striking organization of a large family of human neural cadherin-like cell adhesion genes". Cell. ...

https://en.wikipedia.org/wiki/CELSR3

Many cadherins in the central nervous system exhibit distinct spatial and temporal expression patterns. For example, N-cadherin ... Cadherins are calcium- dependent, homophilic cell adhesion molecules that form complexes with cytosolic partners known as ... Classical cadherins have five extracellular repeating structures which bind calcium, a single transmembrane domain, and an ... An increase in activity at a particular spine leads to the dimerization of N-cadherin which is then cleaved leading the ...

https://en.wikipedia.org/wiki/Synaptic_stabilization

Cadherin EGF LAG seven-pass G-type receptor 1 also known as flamingo homolog 2 or cadherin family member 9 is a protein that in ... The flamingo cadherins are located at the plasma membrane and have nine cadherin domains, seven epidermal growth factor-like ... "Entrez Gene: CELSR1 cadherin, EGF LAG seven-pass G-type receptor 1 (flamingo homolog, Drosophila)". Human CELSR1 genome ... Wu Q, Maniatis T (1999). "A striking organization of a large family of human neural cadherin-like cell adhesion genes". Cell. ...

https://en.wikipedia.org/wiki/CELSR1

Cell-cell adhesion is primarily mediated by cadherin receptors and therefore the adhesome of cell-cell adhesion is referred to ... 2. they directly interact with one of the core adhesome components, such as integrin, cadherin or catenins AND/OR their ... Later, with the emergence of the cadherin-catenin-actin structure they were co-opted into the cadhesome. Whittaker, Charles A ... Zaidel-Bar, Ronen (2013-01-15). "Cadherin adhesome at a glance". J Cell Sci. 126 (2): 373-378. doi:10.1242/jcs.111559. ISSN ...

https://en.wikipedia.org/wiki/Adhesome

Distinct E-cadherin-based complexes regulate cell behaviour through miRNA processing or Src and p120 catenin activity. ... However, in Eph4 cell line, PLEKHA7 is recruited to E-cadherin based AJ by Afadin, independently of p120. PLEKHA7 knockdown ... Therefore, PLEKHA7 stabilises both cadherins and nectins at AJ. Genome-wide association studies suggest that PLEKHA7 is ... Nectins are the second major class of transmembrane adhesion molecules at adherens junctions, besides cadherins. ...

https://en.wikipedia.org/wiki/PLEKHA7

... each encoding 6 extracellular cadherin domains and a cytoplasmic tail that deviates from others in the cadherin superfamily. ... These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins. Their specific functions are unknown ... Wu Q, Maniatis T (Jul 1999). "A striking organization of a large family of human neural cadherin-like cell adhesion genes". ... Nollet F, Kools P, van Roy F (2000). "Phylogenetic analysis of the cadherin superfamily allows identification of six major ...

https://en.wikipedia.org/wiki/PCDHB11

https://en.wikipedia.org/wiki/PCDHB14

Cadherins are present in more complex organisms as well. In mouse embryos, E-cadherin on cell membranes is responsible for the ... This is likely due to distinct cadherins, a calcium-binding membrane protein, expressed by different sponge species and ...

https://en.wikipedia.org/wiki/Cell–cell_recognition

... each encoding 6 extracellular cadherin domains and a cytoplasmic tail that deviates from others in the cadherin superfamily. ... These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins. Their specific functions are unknown ... Wu Q, Maniatis T (1999). "A striking organization of a large family of human neural cadherin-like cell adhesion genes". Cell. ... Nollet F, Kools P, van Roy F (2000). "Phylogenetic analysis of the cadherin superfamily allows identification of six major ...

https://en.wikipedia.org/wiki/PCDHB16

... cadherin 7, type 2 CDH8 - cadherin 8, type 2 CDH9 - cadherin 9, type 2 (T1-cadherin) CDH10 - cadherin 10, type 2 (T2-cadherin) ... CDH2 - N-cadherin (neural): N-cadherins are found in neurons CDH12 - cadherin 12, type 2 (N-cadherin 2) CDH3 - P-cadherin ( ... Proteopedia Cadherin - view cadherin structure in interactive 3D Cadherin domain in PROSITE The cadherin family The Cadherin ... T-cadherin - H-cadherin (heart) CDH15 - M-cadherin (myotubule) CDH16 - KSP-cadherin CDH17 - LI cadherin (liver-intestine) CDH18 ...

https://en.wikipedia.org/wiki/Cadherin

Cadherin_repeat; Cadherin tandem repeat domain. pfam07001. Location:452 → 550. BAT2_N; BAT2 N-terminus. ... Cadherin_repeat; Cadherin tandem repeat domain. pfam07001. Location:452 → 534. BAT2_N; BAT2 N-terminus. ... Cadherin_repeat; Cadherin tandem repeat domain. cl26464. Location:452 → 665. Atrophin-1; Atrophin-1 family. ... Cadherin_repeat; Cadherin tandem repeat domain. PHA03247. Location:454 → 645. PHA03247; large tegument protein UL36; ...

https://www.ncbi.nlm.nih.gov/gene/53841

Compare Anti-M-cadherin Antibody Products from leading suppliers on Biocompare. View specifications, prices, citations, reviews ... Anti-M-cadherin Antibody Products. Listed below are anti-M-cadherin antibodies from multiple suppliers. M-cadherin is a ... reported alias name for the human gene CDH15, or cadherin 15. The 814-amino acid protein has a reported mass of 88,916 ...

https://www.biocompare.com/pfu/110447/soids/22199/Antibodies/M-cadherin

Highly specific and rigorously validated in-house, Pan-Cadherin Antibody (CST #4068) is ready to ship. ... Polyclonal Antibody for studying N-Cadherin/P-Cadherin/E-Cadherin/R-cadherin. Cited in 59 publications. Validated for WB, IP, ... This change in cadherin expression is called the cadherin switch. N-cadherin cooperates with the FGF receptor, leading to ... Pan-Cadherin Antibody detects endogenous levels of E-, VE-, N-, and R-Cadherins.. Species Reactivity:. Human, Mouse, Rat, ...

https://www.cellsignal.com/products/primary-antibodies/pan-cadherin-antibody/4068

... Proc Natl Acad Sci U S A. 2016 Jul 5;113(27): ...

https://pubmed.ncbi.nlm.nih.gov/27342863/

Browse our VE-Cadherin Antibodies all backed by our Guarantee+. ... VE-Cadherin Antibodies. We offer VE-Cadherin Antibodies for use ... anti-cadherin 5, type 2, VE-cadherin (vascular epithelium) antibody, anti-cadherin-5 antibody, anti-CD144 antibody, anti-CD144 ... anti-VE-Cadherin antibody, anti-CDH5 antibody, anti-7B4 antibody, anti-7B4 antigen antibody, anti-cadherin 5, type 2 (vascular ... Choose from our VE-Cadherin polyclonal antibodies and browse our VE-Cadherin monoclonal antibody catalog. ...

https://www.novusbio.com/primary-antibodies/ve-cadherin

One of such regulatory SNPs (rSNPs) is the E-cadherin (CDH1) promoter −160C/A SNP (rs16260) which is known to affect E- ... cadherin promoter transcription by displacing transcription factor binding and has been extensively scrutinized for its ... 4. E-Cadherin −160C/A SNP Affects E-Cadherin Transcriptional Activity. The E-cadherin −160C/A SNP is located at the −160 ... E-Cadherin −160C/A SNP and Gastrointestinal Tract Cancer. E-cadherin −160C/A SNP has been studied most intensively in gastric ...

https://www.hindawi.com/journals/mbi/2014/967565/

In contrast, rescue was ineffective in mice lacking T-cadherin in addition to APN. These data suggest that T-cadherin protects ... We observed extensive colocalization of T-cadherin and APN on cardiomyocytes in vivo. In T-cadherin-deficient mice, APN failed ... T-cadherin is critical for adiponectin-mediated cardioprotection in mice J Clin Invest. 2010 Dec;120(12):4342-52. doi: 10.1172/ ... During ischemia-reperfusion injury, the absence of T-cadherin increased infarct size similar to that in APN-null mice. ...

https://pubmed.ncbi.nlm.nih.gov/21041950/

J:319470 Polanco J, et al., Differential Spatiotemporal Expression of Type I and Type II Cadherins Associated With the ...

http://www.informatics.jax.org/marker/MGI:2442792

005319 B6.129-|i|Cdh1|sup|tm2Kem|/sup||/i|/J |p||strong||/strong|When mice of this strain are bred to a strain expressing Cre recombinase in specific tissues, this strain may useful in studies of various epithelial cell functions.|/p|

https://www.jax.org/strain/005319

Antiparallel protocadherin homodimers use distinct affinity- and specificity-mediating regions in cadherin repeats 1-4. John M. ... Antiparallel protocadherin homodimers use distinct affinity- and specificity-mediating regions in cadherin repeats 1-4 ... Antiparallel protocadherin homodimers use distinct affinity- and specificity-mediating regions in cadherin repeats 1-4 ... Antiparallel protocadherin homodimers use distinct affinity- and specificity-mediating regions in cadherin repeats 1-4 ...

https://www.biorxiv.org/content/10.1101/063289v1

This entry represents the cytoplasmic C-terminal domain of some proto-cadherins. It is this region of the cadherins that allows ... Cadherin_C_2. PFAM accession number:. PF16492. Interpro abstract (IPR032455):. ... The domain within your query sequence starts at position 686 and ends at position 769; the E-value for the Cadherin_C_2 domain ... Cadherins are cell-surface receptors that function in cell adhesion, cell polarity, and tissue morphogenesis [ (PUBMED:1568244 ...

https://smart.embl.de/smart/do_annotation.pl?DOMAIN=Pfam:Cadherin_C_2&START=686&END=769&E_VALUE=1.5e-26&TYPE=PFAM&BLAST=ALTLYLVIALASVSSLFLISVLLFVGMKLCKRARSPSLGVCSVPEGHFPGHLVDVSGTGTLSQSYQYEVCLSGDSGTGEFKFLK

These cells express Cadherin 12 (CDH12, N-Cadherin 2), catenins, and other epithelial markers. CDH12-enriched tumors define ... Here, single nuclei RNA sequencing and spatial profiling identify a cancer cell population expressing Neural Type Cadherin 2 ... These cells express Cadherin 12 (CDH12, N-Cadherin 2), catenins, and other epithelial markers. CDH12-enriched tumors define ... 63/197,129 "USE OF CANCER CELL EXPRESSION OF CADHERIN 12 AND CADHERIN 18 TO TREAT MUSCLE INVASIVE AND METASTATIC BLADDER ...

https://www.nature.com/articles/s41467-021-25103-7?error=cookies_not_supported

M-cadherin-MAGT1 Target ELISA Kit

https://www.biocompare.com/1997-BrowseCategory/browse/gb16/9956/M

Data from: Peptides derived from cadherin juxtamembrane region inhibit platelet function. Golla, Kalyan, Royal College of ... Sequential deletion of amino acids from the N- and C-termini of the inhibitory E-cadherin peptides identified the short ... We conclude that peptides derived from JMD of E-cadherin provide potential lead peptides for the development of anti-thrombotic ... We synthesized overlapping 12-15 amino acids peptides from E- and N-cadherin JMD and assessed their effect on platelet ...

https://datadryad.org/stash/dataset/doi:10.5061/dryad.16m3n

View Mouse P-Cadherin (NP_001032898) VersaClone cDNA in cDNA Clones. ... CAD3; cadherin 3, P-cadherin (placental); cadherin 3, type 1, P-cadherin (placental); Cadherin-3; CDH3; CDHP; CDHPcalcium- ... the cadherin superfamily also includes desmosomal cadherins, protocadherins, 7TM cadherins, FAT family cadherins, and T- ... dependent adhesion protein, placental; HJMD; PCAD; PCadherin; P-Cadherin; PCADP-cadherin; Placental cadherin ...

https://www.rndsystems.com/products/mouse-p-cadherin-np-001032898-versaclone-cdna_rdc1150

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion ... Recombinant protein of human cadherin 1, type 1, E-cadherin (epithelial) (CDH1) ... Transient overexpression lysate of cadherin 1, type 1, E-cadherin (epithelial) (CDH1) ... Transient overexpression lysate of cadherin 1, type 1, E-cadherin (epithelial) (CDH1) ...

https://www.origene.com/catalog/antibodies/primary-antibodies/ta800718/e-cadherin-cdh1-mouse-monoclonal-antibody-clone-id-oti1b11

Analysis of the role of cadherins in retinoblastoma tumor cell adhesion and growth. Lisa Ann Krisak, Thomas Jefferson ... Krisak, Lisa Ann, "Analysis of the role of cadherins in retinoblastoma tumor cell adhesion and growth" (2002). ETD Collection ...

https://jdc.jefferson.edu/dissertations/AAIDP18483/

Cadherins and mechanotransduction by hair cells. / Müller, Ulrich.. In: Current Opinion in Cell Biology, Vol. 20, No. 5, ... Cadherins and mechanotransduction by hair cells. Current Opinion in Cell Biology. 2008 Oct;20(5):557-566. doi: 10.1016/j.ceb. ... Cadherins and mechanotransduction by hair cells. In: Current Opinion in Cell Biology. 2008 ; Vol. 20, No. 5. pp. 557-566. ... Müller, U 2008, Cadherins and mechanotransduction by hair cells, Current Opinion in Cell Biology, vol. 20, no. 5, pp. 557-566 ...

https://jhu.pure.elsevier.com/en/publications/cadherins-and-mechanotransduction-by-hair-cells

... also known as E-cadherin, cadherin-1, CDH1, and UVO is a member of the cadherin superfamily. ... CD324, also known as E-cadherin, cadherin-1, CDH1, and UVO is a member of the cadherin superfamily. It is a calcium-dependent, ... E-Cadherin, Cadherin-1, CDH1, and UVO Isotype Rat IgG1, κ Ave. Rating Submit a Review Product Citations publications MDCK ... PE anti-mouse/human CD324 (E-Cadherin). DECMA-1. FC. Alexa Fluor® 594 anti-mouse/human CD324 (E-Cadherin). DECMA-1. ICC, IHC-P ...

https://www.biolegend.com/it-it/products/purified-anti-mouse-human-cd324-e-cadherin-antibody-9275

Cleavage of E-cadherin by ADAM10 mediates epithelial cell sorting downstream of EphB signalling ...

https://www.irbbarcelona.org/en/about-us/publications/cleavage-e-cadherin-adam10-mediates-epithelial-cell-sorting-downstream-ephb

Both cadherins and catenins are localized in synaptic junctions (Fannon and Colman, 1996; Uchida et al., 1996). When cadherin ... To explore the specific functions of cadherin subtypes, we focused on cad8, a type-II classic cadherin, expressed in the spinal ... Here, we show that cadherin-8 (cad8), a type-II classic cadherin, is important for cold sensation, whose circuitry is ... 2006) Prototypical type I E-cadherin and type II cadherin-7 mediate very distinct adhesiveness through their extracellular ...

https://www.jneurosci.org/content/27/13/3466.full

We studied the alterations of E-cadherin and β-catenin in a set of 50 primary gastric tumours by using loss of heterozygosity ( ... We also analyzed the aberrant E-cadherin mRNAs in the gastric tumours and found that 7 tumours (18%) had aberrant mRNAs in ... Our results support the hypothesis that alterations of E-cadherin and β-catenin play a role in the initiation and progression ... These aberrant mRNAs may produce abnormal E-cadherin molecules, resulting in weak cell-cell adhesion and invasive behaviour of ...

https://bmccancer.biomedcentral.com/articles/10.1186/1471-2407-1-16

PE/Cyanine7 anti-human Cadherin 11 Antibody - Cadherins are calcium-dependent cell adhesion proteins. ... OSF-4, Osteoblast cadherin, OB-cadherin, CDH11, CDHOB, CAD11 Isotype Mouse IgG1, κ Ave. Rating Submit a Review Product ... View All Cadherin 11 Reagents Request Custom Conjugation Description. Clone. Applications. Purified anti-human Cadherin 11. ... Cadherin 11 is a type I transmembrane glycoprotein. It is a type II (atypical) cadherin, based on its lack of a HAV cell ...

https://www.biolegend.com/en-ie/products/pe-cyanine7-anti-human-cadherin-11-antibody-12900

The core of the AJ is the cadherin-catenin complex (Halbleib and Nelson, 2006; Ratheesh and Yap, 2012). Classical cadherins are ... The adhesive properties of classical cadherins are driven by the recruitment of cytosolic catenin proteins to the cadherin tail ... Guo, Z., Neilson, L. J., Zhong, H., Murray, P. S., Zanivan, S. and Zaidel-Bar, R. (2014). E-cadherin interactome complexity and ... Hazan, R. B., Kang, L., Roe, S., Borgen, P. I. and Rimm, D. L. (1997). Vinculin is associated with the E-cadherin adhesion ...

https://www.biorxiv.org/content/10.1101/348953v1.full

Keywords: Cadherins - blood, Breast Neoplasms - pathology, Disease-Free Survival, Kaplan-Meier Estimate, ROC Curve, Solubility ... Abnormal Expression of Serum Soluble E-Cadherin is Correlated with Clinicopathological Features and Prognosis of Breast Cancer ...

https://medscimonit.com/abstract/index/idArt/892049

The objective of this project was to evaluate the biological activity of peptides derived from the EC-4 domain of E-cadherin in ... Inhibition of Cell Adhesion by Peptides Derived from the EC-4 Domain of E-Cadherin. ... of E-cadherin were more effective in inhibiting single cell adhesion than peptides from other domains (EC-1, EC-3) of E- ... cadherin. Peptide 8 had better activity than peptide 6. From overlapping hexapeptides derived from peptide 8, it was found that ...

https://kuscholarworks.ku.edu/handle/1808/21666

... such as VE-cadherin, EphA2, MMP-2 and MMP-9. VE-cadherin belongs to the cadherin family and is specifically expressed in ... Most recent studies have shown that vascular endothelial-cadherin (VE-cadherin), epithelial cell kinase (EphA2), and matrix ... Association of VE-cadherin, EphA2, MMP-2 and MMP-9 proteins with VM. ... Association of VE-cadherin, EphA2, MMP-2 and MMP-9 proteins with VM. ...

https://www.spandidos-publications.com/ijo/45/3/1065

Drosophila epithelial cadherin requires a novel regulatory region for cell migrati ... W hen it comes to cadherin regulation, it is not all about α- and β-catenins. ... Cadherins are used in cell-cell adhesion and migration in a variety of tissues. The cytoplasmic tail of cadherin contacts the ... The full-length cadherin-α-catenin fusion rescued cell-cell interactions and migration in tissues lacking wild-type DE-cadherin ...

https://rupress.org/jcb/article/170/5/697/34191/Controlling-cadherin

Cadherin 10 antibody LS-C752184 is an unconjugated rabbit polyclonal antibody to human Cadherin 10 (CDH10). Validated for ELISA ... Polyclonal Rabbit anti‑Human CDH10 / Cadherin 10 Antibody (WB) LS‑C752184 Polyclonal Rabbit anti‑Human CDH10 / Cadherin 10 ... Cadherin 10 antibody LS-C752184 is an unconjugated rabbit polyclonal antibody to human Cadherin 10 (CDH10). Validated for ELISA ...

https://www.lsbio.com/antibodies/cdh10-antibody-cadherin-10-antibody-elisa-wb-western-ls-c752184/777834

One of such regulatory SNPs (rSNPs) is the E-cadherin (CDH1) promoter −160C/A SNP (rs16260) which is known to affect E-cadherin promoter transcription by displacing transcription factor binding and has been extensively scrutinized for its association with several diseases especially malignancies. (hindawi.com)
CD324, also known as E-cadherin, cadherin-1, CDH1, and UVO is a member of the cadherin superfamily. (biolegend.com)
We define over 350 proteins in this interactome, nearly 200 of which are unique to CDH2 and not part of the E-cadherin (CDH1) interactome. (biorxiv.org)
CDH1 is a protein coding gene that encodes Cadherin-1. (cusabio.com)
Background: Inherited genetic factors such as E-cadherin (CDH1) promoter variants are believed to influence the risk towards sporadic diffuse gastric cancer (DGC). (icr.ac.uk)
The E-cadherin gene (CDH1) variants T340A and L599V in gastric and colorectal cancer patients in Korea. (ox.ac.uk)
INTRODUCTION: Germline mutations in E-cadherin (CDH1) have been reported in families with early onset, diffuse gastric cancer. (ox.ac.uk)
LOXL3 could interact with SNAIL, one of the most important EMT-TFs, thus repressed E-cadherin (CDH1) expression 16 . (researchsquare.com)
The CDH1 gene is located on chromosome 16 and normally encodes for a protein called E-cadherin. (pthealth.ca)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Pan-cadherin Antibody. (cellsignal.com)
Immunohistochemical analysis of paraffin-embedded human bronchioalveolar cell carcinoma using Pan-cadherin Antibody, showing membrane localization. (cellsignal.com)
Each VE-Cadherin Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. (novusbio.com)
Choose from our VE-Cadherin polyclonal antibodies and browse our VE-Cadherin monoclonal antibody catalog. (novusbio.com)
FF-21101 is a human-mouse chimeric monoclonal antibody directed against P-cadherin, conjugated with 111 In for dosimetry and biodistribution and 90 Y for therapy. (aacrjournals.org)
Cadherin 10 antibody LS-C752184 is an unconjugated rabbit polyclonal antibody to human Cadherin 10 (CDH10). (lsbio.com)
Cadherin-6 is a rabbit monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative. (affordableihcinstruments.com)
An anti-N-cadherin antibody can pull out β-catenin, whereas N-cadherin can also be pulled out using an anti-β-catenin antibody. (popcouncil.org)
Calcium-dependent intercellular adhesion of N-cadherin-expressing breast cancer and stromal cells was specifically inhibited by an anti N-cadherin monoclonal antibody. (elsevier.com)
This antibody was affinity purified using E-cadherin (a.a. 774-786) peptide. (ecmbio.com)
In western blots, the antibody detects a 120 kDa band corresponding to E-cadherin in human A431 cells, and does not detect VE-cadherin or N-Cadherin. (ecmbio.com)

Once the cell-cell adhesion between cadherins present in the cell membranes of two different cells has formed, adherens junctions can then be made when protein complexes, usually composed of α-, β-, and γ-catenins, bind to the cytoplasmic portion of the cadherin. (wikipedia.org)
These cells express Cadherin 12 ( CDH12, N-Cadherin 2 ), catenins, and other epithelial markers. (nature.com)
We demonstrate the presence of a previously uncharacterized epithelial cell phenotype marked by high expression of Cadherin 12 ( CDH12 , N-Cadherin 2), catenins and other epithelial markers. (nature.com)
Classical cadherins contain five extracellular cadherin repeats and a conserved cytoplasmic region that interacts with the actin cytoskeleton via catenins. (rndsystems.com)
The adhesive activities of cadherin are regulated by catenins, which bind the cytoplasmic domain of the cadherins. (jneurosci.org)
W hen it comes to cadherin regulation, it is not all about α- and β-catenins. (rupress.org)
Studies in tissue culture cells suggested that modulating the interaction between cadherin and the catenins would alter the adhesion strength of cadherin and facilitate migration. (rupress.org)
Vezatin is a ubiquitous protein of adherens cell±cell junctions, where it interacts with both myosin VIIA and the cadherin±catenins complex. (archives-ouvertes.fr)
Taken together, these data suggest that myosin VIIA, anchored by vezatin to the cadherin±catenins complex, creates a tension force between adherens junctions and the actin cytoskeleton that is expected to strengthen cell±cell adhesion. (archives-ouvertes.fr)
Furthermore, the assembly of AJs between Sertoli and germ cells was associated with a transient induction in the steady-state mRNA and protein levels of cadherins and catenins. (popcouncil.org)
The research presented here demonstrated that BMP-2 not only modulated N-cadherin function by decreasing the overall association of catenins with N-cadherin, but also regulated the intracellular levels and distribution of $\beta$-catenin-levels were maintained in BMP-2 treated cultures, but were redistributed to a cytoplasmic, non-N-cadherin-associated pool. (jefferson.edu)
Cadherins form protein complexes with cytoplasmic proteins (catenins) that convert the specific, homophilic-binding capacity of the extracellular domain into stable cell adhesion. (embl.de)

This gene is a novel mucin-like gene that is a member of the cadherin superfamily. (nih.gov)
The cadherin superfamily comprises a large number of cell surface glycoproteins with one or more cadherin repeats, which are involved in Ca2 + -dependent cell-cell adhesion. (rndsystems.com)
In addition to the classical cadherins, the cadherin superfamily also includes desmosomal cadherins, protocadherins, 7TM cadherins, FAT family cadherins, and T-cadherin. (rndsystems.com)
This gene is a classical cadherin from the cadherin superfamily. (origene.com)
Recent findings indicate that two members of the cadherin superfamily are components of the mechanotransduction machinery in sensory hair cells of the vertebrate inner ear. (elsevier.com)
Member of the cadherin superfamily. (biolegend.com)
These microvilli drive nutrient absorption and are arranged in a hexagonal pattern maintained by intermicrovillar links formed by 2 nonclassical members of the cadherin superfamily of calcium-dependent cell adhesion proteins: protocadherin-24 (PCDH24, also known as CDHR2) and the mucin-like protocadherin (CDHR5). (nicoyalife.com)
This gene encodes a member of the desmoglein family and cadherin cell adhesion molecule superfamily of proteins. (nih.gov)
We aim to understand how the diversity of sequences and structures in the cadherin superfamily contributes to their diverse signaling and adhesion functions mediated in the animal kingdom. (harvard.edu)

While encoding nonpolymorphic tandem repeats rich in proline, serine and threonine similar to mucin proteins, the gene also contains sequence encoding calcium-binding motifs found in all cadherins. (nih.gov)
We studied the alterations of E-cadherin and β-catenin in a set of 50 primary gastric tumours by using loss of heterozygosity (LOH) analysis, gene mutation screening, detection of aberrant transcripts and immunohistochemistry (IHC). (biomedcentral.com)
Myosin VIIa, harmonin and cadherin 23, three Usher I gene products that cooperate to shape the sensory hair cell bundle. (pasteur.fr)
Results: Hypermethylation of E-cadherin gene promoter was detected in 32 (26.7%) of the 120 pterygial specimens. (elsevier.com)
Conclusions: Our study demonstrates E-cadherin gene promoter hypermethylation were associated with low or absent expression of E-cadherin. (elsevier.com)
Exome sequencing led to the identification of a novel homozygous nonsense variant in the first exon of the cadherin-11 gene (CDH11), which results in a prematurely truncated form of the protein. (mpg.de)
We also located the mouse R-cadherin gene to chromosome 2. (houstonmethodist.org)
In total, 7 of 25 diffuse-type cancers harbored genetic alterations in the E-cadherin gene. (unisi.it)
Our observations suggest that inactivation of the E-cadherin gene occurs only in a subset of diffuse-type gastric cancers, as the majority of cases did not contain genetic alterations or identifiable protein abnormalities. (unisi.it)
Failure to express E-cadherin may result from mutations of the E-cadherin gene (HSECAD). (ox.ac.uk)
Results: DNA methylation was detected in 49% tissue samples and 40% peripheral blood samples in p15 gene & 63% tissue samples and 44% peripheral blood samples in E cadherin gene. (journalcra.com)
Kappa coefficient is 0.346 for p15 gene and 0.277 for E cadherin. (journalcra.com)
Elevated expression levels of the CDH2 gene and its protein product, N-cadherin were found in self-seeded cells. (bmj.com)
We show that inhibition of E-cadherin-mediated cell-cell contact in ES cells using gene knockout (Ecad(-/-)), RNA interference (EcadRNAi), or a transhomodimerization-inhibiting peptide (CHAVC) results in cellular proliferation and maintenance of an. (ox.ac.uk)
The protocadherin-α (Pcdha) family of clustered protocadherins consists of 14 cadherin-related molecules generated from a single gene cluster. (jneurosci.org)
James recognized a gene called VE-cadherin that only appears in endothelial cells, making it an ideal marker. (technologyreview.com)
E-cadherin gene polymorphisms and susceptibility to urolithiasis in Iraqi children. (cdc.gov)

Cadherins are a class of type-1 transmembrane proteins, and they are dependent on calcium (Ca2+) ions to function, hence their name. (wikipedia.org)
Cell-cell adhesion is mediated by extracellular cadherin domains, whereas the intracellular cytoplasmic tail associates with numerous adaptors and signaling proteins, collectively referred to as the cadherin adhesome. (wikipedia.org)
The intracellular portion of classical cadherins interacts with a complex of proteins that allows connection to the actin cytoskeleton. (wikipedia.org)
The adaptor proteins that associate with desmosomal cadherins are plakoglobin (related to β {\displaystyle \beta } -catenin), plakophilins (p120 catenin subfamily), and desmoplakins. (wikipedia.org)
Cadherins are synthesized as polypeptides and undergo many post-translational modifications to become the proteins which mediate cell-cell adhesion and recognition. (wikipedia.org)
Protocadherins (Pcdhs) are cell adhesion and signaling proteins used by neurons to develop and maintain neuronal networks, relying on trans homophilic interactions between their extracellular cadherin (EC) repeat domains. (biorxiv.org)
Synthetic JMD peptides derived from cadherin cell adhesion proteins have previously been shown to modulate platelet function. (datadryad.org)
Classic cadherins, another family of cell-cell adhesion proteins, have also been implicated in synaptic contact formation. (jneurosci.org)
In addition, the expression of VE-cadherin, EphA2, MMP-2 and MMP-9 proteins associated with formation of pancreatic cancer vasculogenic mimicry. (spandidos-publications.com)
Rg3 treatment reduced the levels of vasculogenic mimicry in nude mouse xenografts in vitro and in vivo, while the expression of VE-cadherin, EphA2, MMP-2 and MMP-9 mRNA and proteins was downregulated by Rg3 treatment in vitro and in tumor xenografts. (spandidos-publications.com)
Cadherins are calcium-dependent cell adhesion proteins. (biolegend.com)
Classical cadherins are single-pass transmembrane proteins with an extracellular domain that mediates calcium-dependent homotypic interactions. (biorxiv.org)
The adhesive properties of classical cadherins are driven by the recruitment of cytosolic catenin proteins to the cadherin tail: p120-catenin (CTNND1) binds to the juxta-membrane domain and β-catenin (CTNNB1) binds to the distal part of the tail. (biorxiv.org)
A two page magazine article featuring cadherin proteins. (drawbio.com)
Cadherins ( Ca lcium dependant ad hesion molecules) are a class of type-1 transmembrane proteins . (blogspot.com)
One is a group of proteins called cadherins. (blogspot.com)
The aim of the present study was to examine the role of the junctional proteins beta-catenin and E-cadherin during preimplantation in vitro embryo development in sheep, comparing the competence of adult and prepubertal oocytes. (uniss.it)
Introduction Cadherins constitute a large family of cell-cell adhesion proteins that are represented in both vertebrates and invertebrates 1 2 The "classical" type I and type II cadherins are found only in vertebrates and contain an extracellular region consisting of a tandem repeat of five extracellular cadherin immunoglobulin-like domains (EC1-EC5) that extend from the cell surface (Fig. 1A). (scienceofgreathealth.com)
The positions of the strands were determined by the Definition of Secondary Structure of Proteins … This model of human E-cadherin was used to consider possible interaction sites in cadherinCintegrin binding with special reference to solvent-exposed acidic residues on loop structures. (pik-75.info)
All the clustered protocadherin genes encode type I transmembrane proteins with six extracellular cadherin domains ( Wu and Maniatis, 1999 ). (jneurosci.org)
classical cadherins are probably the most important proteins in adhering like cells, (homophilic adhesion), b/c they only like to bind to other cadherins of the same type. (flashcardmachine.com)
these linkages are almost always indirect, and depend on the intracellular anchorage proteins assembling on the tail of the cadherin. (flashcardmachine.com)
Cadherins are a group of transmembrane proteins that serve as the major adhesion molecules located within adherens junctions. (embl.de)
Cadherins are evolutionary related to the desmogleins which are component of intercellular desmosome junctions involved in the interaction of plaque proteins. (embl.de)
However, proteins are designated as members of the broadly defined cadherin family if they have one or more cadherin repeats. (embl.de)
This entry represents the extracellular repeated domains found in cadherins and related proteins. (embl.de)

Each cadherin has a small C-terminal cytoplasmic component, a transmembrane component, and the remaining bulk of the protein is extra-cellular (outside the cell). (wikipedia.org)
The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. (origene.com)
Recombinant protein containing the extracellular cadherin domain 1 of Cadherin 11 fused to maltose binding protein. (biolegend.com)
Removing the cytoplasmic tail of cadherin in the α-catenin fusion protein blocked migration in the ovary, though adhesion was normal. (rupress.org)
Here we show that harmonin, a PDZ domain-containing protein, and cadherin 23 are both present in the growing stereocilia and that they bind to each other. (pasteur.fr)
Moreover, we demonstrate that harmonin b is an F-actin-bundling protein, which is thus likely to anchor cadherin 23 to the stereocilia microfilaments, thereby identifying a novel anchorage mode of the cadherins to the actin cytoskeleton. (pasteur.fr)
Many studies have revealed that E-cadherin associated protein expression decreases in many tumors and pterygia. (elsevier.com)
However, no studies have examined the reason for E-cadherin protein inactivation in pterygia. (elsevier.com)
Therefore, this study aimed to analyze the association of E-cadherin promoter hypermethylation with protein inactivation in pterygial tissues. (elsevier.com)
Methods: E-cadherin methylation-status and the expression of E-cadherin and β-catenin protein were studied using methylation-specific PCR and immunohistochemistry, respectively, on 120 pterygial specimens and 30 normal conjunctivas. (elsevier.com)
A total of 79 (65.8%) pterygial specimens tested positive for E-cadherin protein expression and 41 (34.2%) specimens tested negative. (elsevier.com)
Moreover, loss of E-cadherin protein may contribute to aberrant localization of β-catenin. (elsevier.com)
Herein, we demonstrate that BFT specifically cleaves within 1 min the extracellular domain of the zonula adherens protein, E-cadherin. (elsevier.com)
Furthermore, studies by cross-linking using dithiobis(succinimidylpropionate) confirmed that the cadherin/catenin complex between Sertoli cells as well as between Sertoli and germ cells indeed structurally linked to actin but not to vimentin (an intermediate filament protein) or to tubulin (a microtubule protein). (popcouncil.org)
Thus, the cell binding specificity of R-cadherin is entirely conserved between the two species, suggesting a conserved role for this protein in morphogenesis. (houstonmethodist.org)
RESULTS: Absent or decreased E-cadherin protein expression was found in 27 (38%) of 68 colorectal cancers and the pattern of expression did not differ significantly among the three tumour groups. (ox.ac.uk)
The application of neutron spin echo spectroscopy is described to reveal the activation of nanoscale motions of the actin-binding domain in the cadherin-catenin complex and provides a protein dynamics and entropic explanation for the observed force-sensitive binding behavior of a mechanosensitive protein complex. (semanticscholar.org)
Our results enable us to elucidate fundamental systems of cadherin style and provide book insights regarding the feasible evolutionary systems that underlie the framework and function of the important protein family members. (scienceofgreathealth.com)
It is believed that P-cadherin is not just an adhesion molecule, also acting as a signalling protein involved in breast tissue remodelling, and that its soluble fragment present in human milk might result from the proteolysis of the extracellular domain name [58,59]. (clarkfrancis.com)
test and controlling for plate to plate variability, average values of cell adhesion to mutated E-cadherin-Fc fusion protein were compared with average values of cell adhesion to wild-type E-cadherin-Fc to determine whether adhesion to the mutant was significantly different from adhesion to wild-type. (pik-75.info)
Human E-cadherin was modeled based on GluA3 the murine E-cadherin crystal structure (available from the Protein Data Bank, http://www.rcsb.org/pdb, under accession no. 1EDH) 19. (pik-75.info)
This thesis research investigates the regulation of N-cadherin-dependent cell adhesion by bone morphogenetic protein-2 (BMP-2) during chondrogenesis and the signaling events downstream of BMP-2 and/or N-cadherin required for chondrogenesis. (jefferson.edu)
The thesis research presented here demonstrated that within 24 hours of BMP-2 treatment, C3H10T1/2 cells in high density micromass culture exhibit a 4-fold upregulation of N-cadherin mRNA, and by day 5 an 8-fold upregulation of N-cadherin protein. (jefferson.edu)
BMP-2 upregulation of N-cadherin expression and resultant chondrogenesis required mitogsn activated protein kinase (MAPK) activity and tyrosine phosphorylation as downstream events. (jefferson.edu)
Other laboratories have recently reported that N-cadherin dependent cell adhesion requires intracellular associations with $\alpha $-, $\beta$-, and $\gamma$-catenin, and that the protein adenomatous polyposis coli (APC) may play a role in regulating these interactions. (jefferson.edu)
Haas, Andrew Robert, "Chondrogenic induction by bone morphogenetic protein-2: Involvement of N-cadherin-associated functions" (1997). (jefferson.edu)
He then genetically engineered a green fluorescent protein to turn on in embryonic stem cells only when VE-cadherin is expressed, signaling in real time that the stem cell has differentiated into an endothelial cell. (technologyreview.com)
When there is a mutation, the function of the E-cadherin protein is disrupted, often resulting in cancer. (pthealth.ca)
All lobular carcinoma tumors were defined by loss of cell-to-cell adhesion protein E-cadherin through varied routes of genomic disruption. (cancer.gov)

From these observations, we proposed that each cadherin subtype might be used for linking a selected set of neurons belonging to the same functional group, because of its homophilic binding specificity. (jneurosci.org)
Cadherins are membrane glycoproteins that mediate homophilic cell-cell adhesion and play critical roles in cell differentiation and morphogenesis. (affordableihcinstruments.com)
Besides mediating cell-cell adhesion through homophilic interactions, E-cadherin mediates contact inhibition of cell growth, and loss of E-cadherin is associated with tumorigenesis. (huabio.com)
Cadherins cluster to form foci of homophilic binding units. (ecmbio.com)
Here, we show that cadherin-11 (also known as osteoblast-cadherin) was highly expressed in prostate cancer cell line derived from bone metastases and had strong homophilic binding to recombinant cadherin-11 in vitro. (elsevier.com)
Sotomayor, M. Heterophilic and homophilic cadherin interactions in intestinal intermicrovillar links are species dependent. (nicoyalife.com)
Cadherins are expressed in tissue-restricted patterns and typically mediate homophilic adhesion. (pik-75.info)
site is distinct from the homophilic binding site on E-cadherin. (pik-75.info)
The extracellular domains of cadherins form parallel dimers that possess intrinsic homophilic-binding activity. (embl.de)

The extracellular architecture of adherens junctions revealed by crystal structures of type I cadherins. (drawbio.com)
The classic cadherin subfamily includes N-, P-, R-, B- and E-cadherins as well as about ten other members which are found in adherens junctions (AJ), E-cadherin is found at the junctions of epithelial cells. (huabio.com)
Is the cadherin/catenin complex a functional unit of cell-cell actin-based adherens junctions in the rat testis? (popcouncil.org)
Much controversy exists regarding the presence of the cadherin/catenin complex and its intracellular attachment site in the testis, which is the functional unit for actin-based cell-cell adherens junctions (AJs) in multiple epithelia. (popcouncil.org)
How is cadherin recruited to the adherens junction? (mechanobio.info)
Although this process is fundamental to the development of multicellular organisms, it is also a key occurrence in many diseases, including cancers of epithelial origin E-cadherin is a central component of adherens junctions (AJs), which act as structural and signaling hubs in epithelial cells that oppose EMT. (elsevier.com)
Intercellullar junctions formed by cadherins, including desmosomes and adherens junctions, comprise two dimensional arrays of "trans" dimers formed between monomers emanating from opposing cell surfaces. (elsevier.com)
Vascular endothelial (VE)-cadherin, the major adherens junction adhesion molecule in endothelial cells, interacts with p120-catenin and β-catenin through its cytoplasmic tail. (elsevier.com)
Molecular and functional analysis of cadherin-based adherens junctions. (embl.de)
Adherens junctions are specialized forms of cadherin-based adhesive contacts important for tissue organization in developing and adult organisms. (embl.de)
Role of N-Cadherin cis and trans interfaces in the dynamics of adherens junctions in living cells. (mpg.de)

Renewed analysis with respect to putative relations to Wnt signaling revealed that P-cadherin (CDH3) had three mutations in the coding region of the extracellular domain and was associated with high Wnt signaling. (uu.nl)
Loss of the cell adhesion molecule E-cadherin has been observed in a variety of human carcinomas, and germline E-cadherin mutations have been found in several familial cases of diffuse gastric cancer. (unisi.it)
We use these findings to design site-directed mutations which transform a monomeric EC2-EC3 domain cadherin construct into a strand-swapped dimer. (scienceofgreathealth.com)
E-cadherin germline mutations in familial gastric cancer. (cancer.gov)
germ line mutations of which cadherin can predispose one to familial gastric carcinoma? (flashcardmachine.com)

This linkage between transmembranous cadherins and actin filaments of the cytoskeleton is necessary to form strong cell-cell adhesion. (biomedcentral.com)
The cytoplasmic tail of cadherin contacts the actin cytoskeleton through α- and β-catenin. (rupress.org)
If the link between DE-cadherin and the actin cytoskeleton needs to be tempered in vivo, the tempering must happen downstream of α-catenin. (rupress.org)
The cytoplasmic region is highly conserved in sequence and has been shown experimentally to regulate the cell-cell binding function of the extracellular domain of E-cadherin, possibly through interaction with the cytoskeleton. (ecmbio.com)
beta catenin is an element of the anchoring complex between cadherins+cytoskeleton. (flashcardmachine.com)

Strategies Components Mouse anti-N-cadherin antibodies and 3,3′,5,5′-Tetramethylbenzidine (TMB) alternative for ELISA had been bought from Sigma-Aldrich (Poznan, Poland). (fk866.net)

Reduced expression of E-cadherin and β-catenin was identified at the frequency of 42% and 28%, respectively. (biomedcentral.com)
An association was found between reduced expression of E-cadherin and β-catenin. (biomedcentral.com)
Moreover, an association was detected between reduced expression of E-cadherin and diffuse histotype. (biomedcentral.com)
Altered vasculogenic mimicry formation was assessed using immunohistochemistry and PAS staining and associated with the expression of vascular endothelial-cadherin (VE-cadherin), epithelial cell kinase (EphA2), matrix metalloproteinase (MMP)-2 and MMP-9. (spandidos-publications.com)
In conclusion, ginsenoside Rg3 effectively inhibited the formation of pancreatic cancer vasculogenic mimicry by downregulating the expression of VE-cadherin, EphA2, MMP9 and MMP2. (spandidos-publications.com)
The expression of this particular cadherin in osteoblastic cell lines along with its upregulation during differentiation suggests a specific function in bone development and maintenance. (biolegend.com)
Pre-treatment tumor samples were assessed for P-cadherin expression by immunohistochemistry (IHC). (aacrjournals.org)
The revelation of the genetic and molecularbasis of gastric cancer has helped the development of targetedtherapies which has the potential to improve survival.Decreased expression of Epithelial cadherin (E-cadherin)in gastric carcinoma is one such genetic alteration whichcould help in targeted therapy and prognostication. (who.int)
Theobjective of the present study was to identify E- cadherinimmunohistochemical expression in gastric carcinoma and itscorrelation with histopathological features.Material and Methods: Gastric biopsies and surgicalspecimens from a tertiary care center in South Indiawere included and assessed by light microscopy andimmunohistochemistry (IHC).Results: Aberrant E-cadherin staining was seen in 42.8% ofgastric adenocarcinoma. (who.int)
In this study, there was no significant associationbetween E-cadherin with age, gender, nature of the specimen,site of the tumor, size of the tumor, lymph node status andtumor invasion.Conclusion: This study emphasizes the importance ofidentification of aberrant E-cadherin expression in gastriccarcinomas which in turn helps in selecting patients for noveltherapies which might improve the prognosis of this gravedisease. (who.int)
The expression of Cadherin-6 is associated with tumor progression in renal cell carcinoma. (affordableihcinstruments.com)
E-cadherin expression is frequently down-regulated or extinguished in malignancy which strongly correlates with poor prognosis. (huabio.com)
This study aimed to detect the expression of E-Cadherin in endometrial tumors, using immunohistochemistry. (sustech.edu)
Positive expression of E-Cadherin was found in 17 (42.5%) sample, and negative expression found in 3 (7.5%) sample in both benign and malignant tumors. (sustech.edu)
This result showed insignificant association of E-Cadherin expression and endometrial tumors (P. value = 1.00). (sustech.edu)
The study concludes there is no association between E-cadherin expression and endometrial tumors. (sustech.edu)
To examine whether PKCK2 activity could determine the type of cadherin expressed, we first increased intracellular PKCK2 activity in TE2 cells by overexpressing the PKCK2a catalytic subunit using lentivirus and found that high PKCK2 activity could switch cadherin expression from type E to N and confer anoikis resistance. (elsevier.com)
In addition, N-cadherin expression correlated with PKB/Akt activation and increased invasiveness. (elsevier.com)
In breast and other tumor types, loss of E-cadherin expression has been seen in high grade tumors and correlates with increased invasiveness. (elsevier.com)
Here we show high levels of expression of N-cadherin in the most invasive breast cancer cell lines which was inversely correlated with their expression of E-cadherin. (elsevier.com)
Although the function of this cadherin (also termed Osteoblast-Cadherin) is not completely understood, its prevalent expression in osteoblastic cell lines and up-regulation during differentiation suggest a specific function in bone formation and development. (mpg.de)
Allele loss, replication errors and loss of expression of E-cadherin in colorectal cancers. (ox.ac.uk)
BACKGROUND: Loss of E-cadherin expression has been implicated in the development of invasive characteristics in colorectal carcinomas. (ox.ac.uk)
and to examine differences in E-cadherin expression and genetic changes at HSECAD between three different groups of colorectal cancer--replication error positive (RER+) sporadic cancers, RER--sporadic cancers and ulcerative colitis associated cancers. (ox.ac.uk)
Overall, there was no correlation between allelic loss or exon 16 replication errors and immunohistochemical E-cadherin expression. (ox.ac.uk)
CONCLUSIONS: (1) Loss of E-cadherin expression probably does not occur as a result of mutation at the HSECAD locus in colorectal cancers. (ox.ac.uk)
However, the incidence of PC3 metastasis to bone in this model was reduced greatly when the expression of cadherin-11 by those cells was silenced. (elsevier.com)
The clinical relevance of cadherin-11 in prostate cancer metastases was further studied by examining the expression of cadherin-11 in human prostate cancer specimens. (elsevier.com)
Cadherin-11 was not expressed by normal prostate epithelial cells but was detected in prostate cancer, with its expression increasing from primary to metastatic disease in lymph nodes and especially bone. (elsevier.com)
Cadherin-11 expression was not detected in metastatic lesions that occur in other organs. (elsevier.com)
We therefore studied the expression and distribution of E-cadherin during the major transition from maternal to embryonic genome. (uniss.it)
Considering the broad expression of E-cadherin in epithelia, this exploratory study encourages further evaluation of the 163+ 37235A-allele as a susceptibility variant in other carcinomas. (icr.ac.uk)
Elevated expression level of vascular endothelial (VE)-cadherin has been implicated in cancer neovascularization, growth, and progression. (jispcd.org)
There were statistically significant differences between tumor grade and the expression levels of VE-cadherin ( P = 0.000), between tumor grade and VM formation ( P = 0.000), and also between tumor grade and microvessel density (MVD) ( P = 0.000). (jispcd.org)
Our results may disclose a definite relationship between VE-cadherin expression level, VM, epithelial-mesenchymal transition, cancer stem cells, and MVD in MEC samples. (jispcd.org)
2004. The dietary phytochemical chlorophyllin alters E-cadherin and beta-catenin expression in human colon cancer cells. . (oregonstate.edu)
Cadherins expression profile in high quality and in infiltrative tumours was equivalent, almost all expressing P-cadherin, of E-cadherin expression position regardless. (clarkfrancis.com)
Furthermore, the expression of P-cadherin was associated to a 8 almost.5 odds ratio for vascular invasion. (clarkfrancis.com)
neither there was a statistical significant association between the expression of E-cadherin in main tumours and in their neoplastic intravascular emboli and lymph node metastases. (clarkfrancis.com)
The P-cadherin/E-cadherin combined expression patterns were significantly associated with histological grade of carcinomas, mode of growth and presence of necrosis. (clarkfrancis.com)
Pulsatile flow-induced shear stress in large vessels affects VE-Cadherin and Neuregulin-1 expression in endothelial cells. (aps.org)
We investigated the effect of realistic healthy and unhealthy large vessel shear stresses on the endothelial cells' expression of vascular endothelial cadherin (VE-Cad) and neuregulin-1 (NRG-1). (aps.org)
Exogenous expression of H-cadherin in CHO cells regulates contact inhibition of cell growth by inducing p21 expression. (elsevier.com)
Dive into the research topics of 'Exogenous expression of H-cadherin in CHO cells regulates contact inhibition of cell growth by inducing p21 expression. (elsevier.com)
Compared with primary tumors, N-cadherin expression was significantly increased in matched recurrent tumor tissues. (bmj.com)
which cancers is reduced surface expression of E-cadherin noted in? (flashcardmachine.com)
and (3) recombinant expression of a dominant negative N-cadherin mutant molecule inhibited chondrogenesis. (jefferson.edu)
The results presented here indicate that BMP-2 requires the upregulation of N-cadherin expression and function to induce chondrogenesis, and alterations in N-cadherin dependent cell-cell adhesion at the cadherin-catenin complex level occur as cells begin to differentiate. (jefferson.edu)
We have shown that E Cadherin expression closely associates with tumor progression and survival. (marian.edu)
In the presence of CsA, E-cadherin is not effectively cleared from the membrane, β-catenin does not enter the nucleus and there is no effective expression of Bra. (biomedcentral.com)
Cancer cells that were cultured with CAFs had down-regulated E-cadherin expression compared to cancer cells that were cultured with normal fibroblasts. (news-medical.net)

Binding of p-120 catenin and β {\displaystyle \beta } -catenin to the homodimer increases the stability of the classical cadherin. (wikipedia.org)
We analysed the concentration of beta-catenin and E-cadherin in immature and in vitro-matured oocytes. (uniss.it)

The loss of E-cadherin from the plasma membrane is an early indication of EMT and a marker of poor prognosis in many cancers making the trafficking of E-cadherin an area of great interest. (elsevier.com)

The aim of the study was to assess the accuracy of some specific biochemical indicators in discriminating between Helicobacter pylori-associated gastritis and H. pylori-associated stomach cancer [‎serum gastrin level, serum soluble E-cadherin and tissue COX-2 activity, as well as serodiagnostic markers for H. pylori infection]‎ in order to find a simple diagnostic test that can reasonably predict the development of gastric cancer. (who.int)
We suggest that close follow-up together with periodic endoscopic examination for all patients with persistent H. pylori infection and serum soluble E-cadherin level above 5 µg/mL is essential. (who.int)

In structure, they share cadherin repeats, which are the extracellular Ca2+-binding domains. (wikipedia.org)
Similar to classical cadherins, desmosomal cadherins have a single transmembrane domain, five EC repeats, and an intracellular domain. (wikipedia.org)
Because cadherins are Ca2+ dependent, they have five tandem extracellular domain repeats that act as the binding site for Ca2+ ions. (wikipedia.org)
Cadherin repeats. (embl.de)
Cadherin domains occur as repeats in the extracellular regions which are thought to mediate cell-cell contact when bound to calcium. (embl.de)

Abstract Cadherins are cell-adhesion molecules that control morphogenesis, cell migration, and cell shape changes during multiple developmental processes. (mpg.de)
abstract = "R-cadherin was originally identified as a chicken cadherin expressed by the retina. (houstonmethodist.org)

Our results support the hypothesis that alterations of E-cadherin and β-catenin play a role in the initiation and progression of gastric cancer. (biomedcentral.com)

Western blot image of human A431 cells that were probed with rabbit polyclonal anti-E-Cadherin (a.a. 774-786) at 1:250 (lane 1), 1:1000 (lane 2), and 1:4000 (lane 3) or mouse monoclonal anti-E-cadherin (Cytoplasmic) at 1:250 (lane 4) and 1:1000 (lane 5). (ecmbio.com)

Listed below are anti-M-cadherin antibodies from multiple suppliers. (biocompare.com)
Our VE-Cadherin Antibodies can be used in a variety of model species: Human, Mouse, Rat. (novusbio.com)
describe the preparation and application of antibodies directed against a synthetic, 24 amino acid long, peptide corresponding to the conserved cytoplasmic C terminus of N-cadherin. (zfin.org)
They demonstrate that the antibodies to the synthetic peptide react extensively with all known members of the cadherin family and, in addition, recognize novel cadherins in a variety of cells and tissues, suggesting that these antibodies indeed exhibit pan-cadherin reactivity. (zfin.org)

Overexpression of P-cadherin, a calcium-dependent cell-to-cell adhesion glycoprotein, correlates with increased tumor cell invasiveness in breast, colon, lung and pancreatic tumors. (aacrjournals.org)
Within this series, a substantial Wortmannin ic50 overexpression of P-cadherin by luminal epithelial cells was seen in carcinomas, in comparison with harmless mammary tumours. (clarkfrancis.com)
In vivo experimental results showed that overexpression of N-cadherin promoted tumor self-seeding and facilitated the survival of CTCs. (bmj.com)

It is a type II (atypical) cadherin, based on its lack of a HAV cell adhesion recognition sequence that is specific to type I cadherins. (biolegend.com)
Conserved anchor residues - Trp2 in type I cadherins or Trp2 and Trp4 in type II - dock into a complementary pocket in the partner molecule 3-6. (scienceofgreathealth.com)
The A* strand which comprises residues 1-3 represents the N-terminal segment of a strand that in type I cadherins spans residues 1-10 and includes a break at residues 4-6 due to the presence of prolines at positions 5 and 6 which provides a hinge that mediates conformational changes necessary for strand swapping (Fig. 1). (scienceofgreathealth.com)

Both diffuse and intestinal-type tumors exhibited low rates of LOH, suggesting that allelic loss at the locus is not a common mechanism for E-cadherin inactivation during gastric tumorigenesis. (unisi.it)

The Crystal Structure of Human E-cadherin Domains 1 and 2, and Comparison with other Cadherins in the Context of Adhesion Mechanism. (drawbio.com)
E-cadherin synthetic peptide corresponding to amino acids in the C-terminal region in human E-cadherin. (ecmbio.com)
Modeling of Human E-Cadherin. (pik-75.info)
Using the program O (T.A. Jones, Uppsala University, and M. Kjelgaard, Aahus University), sequence substitution of human E-cadherin residues into the murine E-cadherin structure was performed. (pik-75.info)
The side chain conformation of human E-cadherin residues was chosen Roflumilast to be similar to that of the murine E-cadherin residues while potential close contact was avoided. (pik-75.info)
Results Sequence Analysis and Modeling of Human E-Cadherin. (pik-75.info)
The amino acid sequence of the first domain of human E-cadherin was aligned with that of murine E- and N-cadherin, and there did not appear to be any deletions or insertions (Fig. 1 A). Domain 1 of human E-cadherin shares 89% amino acid sequence identity with domain 1 of murine E-cadherin. (pik-75.info)
Therefore, the structure of human E-cadherin is predicted to be very similar to that of murine E-cadherin. (pik-75.info)
As the core structure of the Roflumilast Roflumilast barrel is predicted to be highly conserved, we developed a model of human E-cadherin based on the murine E-cadherin structure (Fig. 1 B). Figure 1 Structural analysis of human E-cadherin. (pik-75.info)
A) Amino acid alignment of domain 1 of human E-cadherin with human P-cadherin and murine E- and N-cadherin. (pik-75.info)

Cadherin 2 (CDH2). (biolegend.com)
We combine quantitative mass spectrometry with proximity labeling to identify the N-cadherin (CDH2) interactome. (biorxiv.org)
Summary Statement Proximity proteomics reveals a specific and specialized N-cadherin (CDH2) interactome along the cell-cell contacts of primary cardiomyocytes. (biorxiv.org)

Classical cadherins maintain the tone of tissues by forming a homodimer in cis while desmosomal cadherins are heterodimeric. (wikipedia.org)
Although classical cadherins take a role in cell layer formation and structure formation, desmosomal cadherins focus on resisting cell damage. (wikipedia.org)
Desmosomal cadherins are responsible to maintain the function of desmosomes that is to overturn the mechanical stress of the tissues. (wikipedia.org)
Two types of desmosomal cadherins exist, and they are called desmogleins and desmocollins that contain an intracellular anchor and cadherin like sequence (ICS). (wikipedia.org)

E-cadherin may be a marker for both tumor metastasis and prognosis. (elsevier.com)
The combined results suggest a functional role for N-cadherin in cohesion of breast tumor cells which, in addition promotes their interaction with the surrounding stromal cells, thereby facilitating invasion and metastasis. (elsevier.com)
The current study aimed to study the presence of VE-cadherin in VM channels and tumor cells in different grades of MEC. (jispcd.org)
Soluble N-cadherin triggered NK cell functional exhaustion by interacting with the killer cell lectin-like receptor subfamily G member 1 (KLRG1) receptor and therefore protected tumor cells from NK cell killing in the circulation. (bmj.com)
Conclusion Together, our findings illustrate an unknown mechanism by which CTCs evaded NK cell-mediated immune surveillance, and indicate that targeting N-cadherin is an effective strategy to prevent CTCs from homing to primary tumor. (bmj.com)
The cross-talk between tumor cells and natural killer (NK) cells in the circulation can trigger NK cell functional exhaustion via N-cadherin/KLRG1 axis, thus enabling CTCs to avoid immune attack. (bmj.com)
when tumor stem cells derived from GBM were grown in the presence of human endothelial cells, a fraction of them acquired endothelial markers (CD31, CD105, VE-cadherin, and vWF). (hindawi.com)

Calcium-dependent cell adhesion molecules (cadherins) are involved in maintaining the epithelial structure of a number of tissues including the mammary gland. (elsevier.com)
Cadherins are transmembrane glycoproteins vital in calcium-dependent cell-cell adhesion during tissue differentiation. (ecmbio.com)
Background Cadherins are calcium-dependent cell-to-cell adhesion glycoproteins playing a critical role in the formation and maintenance of normal tissue architecture. (clarkfrancis.com)

There are multiple classes of cadherin molecules, each designated with a prefix (in general, noting the types of tissue with which it is associated). (wikipedia.org)
These aberrant mRNAs may produce abnormal E-cadherin molecules, resulting in weak cell-cell adhesion and invasive behaviour of carcinoma cells. (biomedcentral.com)
A key event is the strand-swap dimerization of the extracellular ectodomains of two cadherin molecules from apposing cells. (elsevier.com)
Furthermore, whether germ and Sertoli cells are equipped with the necessary AJ-associated signaling molecules to regulate this cadherin/catenin complex during spermatogenesis is not known. (popcouncil.org)
Experiments in epithelial cells expressing cadherin tagged to a photoconvertible fluropohore for easy tracking, have shown AJs to be highly dynamic structures that continuously turnover cadherin molecules. (mechanobio.info)

Using micropatterning to isolate the role of cell-cell adhesion from cell-extracellular matrix adhesion, we demonstrate that endothelial cells required cell-cell contact and vascular endothelial cadherin engagement to transduce stretch into proliferative signals. (princeton.edu)
We used EGFR FISH Probe to detect EGFR amplification and anti-CD31, CD105, VE-cadherin, and vWF to identify endothelial cells. (hindawi.com)
In the bracket (right), en face staining, frozen cryosection of aortic ring, phase contrast micrograph of mouse aortic endothelial cells from EC-PhAM mice (MAEC EC-PhAM ), epifluorescence image of MAEC EC-PhAM , en face lesser curvature, and en face thoracic aorta are shown. (jci.org)

One current model proposes that cells distinguish cadherin subtypes based on kinetic specificity rather than thermodynamic specificity, as different types of cadherin homotypic bonds have different lifetimes. (wikipedia.org)
This mechanism, applicable to type I classical cadherins, confers high specificity and fast association rates. (elsevier.com)
To examine the binding specificity of mouse R-cadherin, L cells expressing this cadherin (mRL) were mixed with L cells expressing chicken R-cadherin (cRL), mouse N-cadherin (mNL), mouse E-cadherin (mEL) and mouse P-cadherin (mPL). (houstonmethodist.org)
The dashed box indicates … A considerable body of evidence demonstrates that cell-cell adhesive specificity is determined by the identity of the EC1 domain which contains the cadherin-cadherin binding interface 5 6 8 Vincristine sulfate Cadherins within the same subfamily (eg. (scienceofgreathealth.com)

Here, we report that the glycosyl phosphatidylinositol-anchored cell surface glycoprotein T-cadherin (encoded by Cdh13) protects against cardiac stress through its association with APN in mice. (nih.gov)
Cadherin 11 is a type I transmembrane glycoprotein. (biolegend.com)

The E-cadherin-catenin complex plays a crucial role in epithelial cell-cell adhesion and in the maintenance of tissue architecture. (biomedcentral.com)
An intact E-cadherin - catenin complex is required for maintenance of normal intercellular adhesion. (biomedcentral.com)
β-Catenin, in turn recruits αE-catenin (CTNNA1) to the cadherin-catenin complex. (biorxiv.org)
The E-cadherin-catenin complex in tumour metastasis: structure, function and regulation. (drawbio.com)
Is the cadherin/catenin complex a functional unit of cell-cell actin-b" by Nikki P.Y. Lee, Dolores D. Mruk et al. (popcouncil.org)
These analyses reveal, to our knowledge for the first time, that the testis may indeed be using the cadherin/catenin complex as one of the functional units to regulate AJ dynamics between Sertoli and germ cells in addition to α 6 β 1 , integrin and the nectin/afadin complex. (popcouncil.org)
These results thus unequivocally demonstrate that the cadherin/catenin complex, which can be up-regulated by testosterone, is indeed present between Sertoli and germ cells and is used for the assembly of functional AJs. (popcouncil.org)

protocadherins are located in the brain, (50+ non-classical cadherins in brain alone), desmocollins and desmogleins form desmosome junctions, and T-cadherins have signalling functions in nerve and muscle cells. (flashcardmachine.com)

Here we have determined crystal structures of P-cadherin in six different conformational states to elaborate a motion picture of its adhesive dimerization at the atomic level. (elsevier.com)
The snapshots revealed that cell-adhesive dimerization is facilitated by several intermediate states collectively termed X-dimer in analogy to other classical cadherins. (elsevier.com)
Based on previous studies and on the combined structural, kinetic, thermodynamic, biochemical, and cellular data reported herein, we propose that the adhesive dimerization of human P-cadherin is achieved by a stepwise mechanism analogous to that of assembly chaperones. (elsevier.com)
Trans cadherin interactions result in an adhesive dimer interface between the interacting EC1 domains. (mechanobio.info)
Here we use bioengineering approaches to identify the roles of cadherin-catenin interactions in promoting strong cellular adhesion and the ability of the cells to spread on an adhesive surface. (elsevier.com)
Surprisingly, p120 binding to the cadherin tail had no effect on the strength of adhesion when the available adhesive area was limited. (elsevier.com)
Instead, the binding of VE-cadherin to p120 regulates adhesive contact area in a Rac1-dependent manner. (elsevier.com)
Cadherins depend on calcium for their function: calcium ions bind to specific residues in each cadherin repeat to ensure its proper folding, to confer rigidity upon the extracellular domain and is essential for cadherin adhesive function and for protection against protease digestion. (embl.de)

Most recent studies have shown that vascular endothelial-cadherin (VE-cadherin), epithelial cell kinase (EphA2), and matrix metalloproteinase (MMPs) play a crucial role in VM formation ( 13 - 21 ). (spandidos-publications.com)
Together, these data suggest that vascular endothelial cadherin has an important role in mechanotransduction and that endothelial and smooth muscle cells use different mechanisms to respond to stretch. (princeton.edu)

The full-length cadherin-α-catenin fusion rescued cell-cell interactions and migration in tissues lacking wild-type DE-cadherin or β-catenin. (rupress.org)
It is this mature, curved form of cadherin that engages in trans interactions. (mechanobio.info)
The outermost EC domain (EC1) of cadherins engage in trans interactions by forming a dimer interface. (mechanobio.info)
Here we report Monte Carlo simulations performed on a 2D lattice constructed to account for the anisotropy in cadherin cis interactions. (elsevier.com)
Cytoplasmic interactions can influence the function of the ectodomain by a number of potential mechanisms, including redistribution of binding sites into clusters, providing cytoskeletal anchorage, and mediating physiological regulation of cadherin function. (embl.de)

The Ca$\sp{2+}$-dependent cell adhesion molecule, N-cadherin, plays a functional role in chondrogenesis. (jefferson.edu)
As E-cadherin is an epithelial adhesion molecule, this change allows the cancer cells cultured with CAF to migrate more easily, contributing to metastasis. (news-medical.net)

Box plots representing the levels of soluble N-cadherin (sN-CAD) in the sera of 87 control subjects and 73 sarcoma patients. (biomedcentral.com)
Background An increasing variety of publications are suggesting that galectin-3 (Gal-3) and soluble cadherin fragments, such as for example E-cadherin (sE-CAD) and N-cadherin (sN-CAD), could be considered as cancers markers. (fk866.net)

Typical cadherins are different from other types of cadherins and consist of one or more extracellular repeat domains. (wikipedia.org)
The extracellular domains of CDH23 and PCDH15 differ in their structure from classical cadherins and their cytoplasmic domains bind to distinct effectors, suggesting that evolutionary pressures have shaped the two cadherins for their function in mechanotransduction. (elsevier.com)
The results showed that peptide 6 (LVVQAADLQG) derived from the EC-2 domain and peptide 8 derived from the EC-4 domain (YTALIIATDN) of E-cadherin were more effective in inhibiting single cell adhesion than peptides from other domains (EC-1, EC-3) of E-cadherin. (ku.edu)
In the closed monomers of cadherin, β-strand A of the EC1 domain is held under conformational strain through anchoring via tryptophan-2 at one end and Ca2+-bound-glutamine-11 at the other end This conformation favors the 'swapping' of β-strands on apposing cadherin EC1 domains. (mechanobio.info)
Cell adhesion simply by classical cadherins is mediated by dimerization of their EC1 domains through the "swapping" of N-terminal β-strands. (scienceofgreathealth.com)
Cadherin ectodomains bind between cells through the interaction of their EC1 domains which exchange or swap their N-terminal β-strand (the A* strand). (scienceofgreathealth.com)
Interestingly, all 11 substituted residues are solvent-exposed based on the crystal structure of the two NH2-terminal domains of murine E-cadherin 19. (pik-75.info)
The seven strands in the cadherin domain form two antiparallel sheets, one formed by strands D, E, and B and the other by strands A, G, F, and C. Without the conserved intersheet disulfide bond present in Ig domains, the strands in cadherin domains have a more cylindrical arrangement that has been termed a barrel. (pik-75.info)
Residue D29 is conserved among cadherin domains (Fig. 1 A). Based on our model, Roflumilast the side chain of D29 is predicted to point into the core of the structure and hydrogen bond to Y36. (pik-75.info)
CADG : Dystroglycan-type cadherin-like domains. (embl-heidelberg.de)
how do the intracellular domains of cadherins work? (flashcardmachine.com)
Crystal structures have revealed that multiple cadherin domains form Ca2+-dependent rod-like structures with a conserved Ca2+-binding pocket at the domain-domain interface. (embl.de)

We also analyzed the aberrant E-cadherin mRNAs in the gastric tumours and found that 7 tumours (18%) had aberrant mRNAs in addition to the normal mRNA. (biomedcentral.com)
Aberrant localization of β-catenin was higher in the E-cadherin negative group than in E-cadherin positive group. (elsevier.com)
p15 and E-cadherin aberrant methylation are very frequent in adult acute monocytic leukemia. (jahjournal.org)
Both cadherins had been found to become co-expressed in carcinomas with aberrant P-cadherin appearance and conserved E-cadherin. (clarkfrancis.com)
Moreover, this implies that, in queens, the aberrant appearance of P-cadherin is certainly an improved marker of mammary carcinomas intense behaviour compared to the reduced amount of E-cadherin appearance. (clarkfrancis.com)

To demonstrate the role of N-cadherin (N-cad) in maintaining the progenitor status of primary human limbal epithelial cells in vitro. (fujita-hu.ac.jp)

The objective of this project was to evaluate the biological activity of peptides derived from the EC-4 domain of E-cadherin in inhibiting E-cadherin-mediated cell-cell adhesion. (ku.edu)

Here, we show that planar polarization of flows emerges from polarized fluctuations in the levels of E-cadherin clusters that produce transient and oscillating asymmetries of coupling. (pasteur.fr)

The cadherin family is essential in maintaining the cell-cell contact and regulating cytoskeletal complexes. (wikipedia.org)
It has been observed that cells containing a specific cadherin subtype tend to cluster together to the exclusion of other types, both in cell culture and during development. (wikipedia.org)
The homodimeric cadherins create cell-cell adhesion with cadherins present in the membranes of other cells through changing conformation from cis-dimers to trans-dimers. (wikipedia.org)
It is this region of the cadherins that allows cell-adhesion and the essential feature of metazoan multicellularity. (embl.de)
In addition to cell adhesion, cadherins also mediate morphogenesis, cytoskeletal organization, and cell migration. (rndsystems.com)
These studies show that cadherin 23 (CDH23) and protocadherin 15 (PCDH15) form several of the extracellular filaments that connect the stereocilia and kinocilium of a hair cell into a bundle. (elsevier.com)
Müller, U 2008, ' Cadherins and mechanotransduction by hair cells ', Current Opinion in Cell Biology , vol. 20, no. 5, pp. 557-566. (elsevier.com)
It has the ability to inhibit cell proliferation by the upregulation of p27 involved in the cell cycle regulation [ 5 ], although the mechanism by which E-cadherin regulates p27 is still unclear. (biomedcentral.com)
Classic cadherins, comprising multiple subtypes, mediate selective cell-cell adhesion based on their subtype-specific binding nature. (jneurosci.org)
Human lung cancer cell line H460 cells were stained with Cadherin 11 (clone 16G5) PE/Cyanine7 (filled histogram) or mouse IgG1, κ PE/Cyanine7 isotype control (open histogram). (biolegend.com)
Cadherins are used in cell-cell adhesion and migration in a variety of tissues. (rupress.org)
Orderly assembly of classical cadherins governs cell adhesion and tissue maintenance. (elsevier.com)
It has been found to be related to fetal kidney development and has been identified as a major cadherin in renal proximal tubules where conventional renal cell carcinoma originates. (affordableihcinstruments.com)
Western blot analysis of E-Cadherin on A431 cell lysate. (huabio.com)
E-cadherin is shown here to be a cellular substrate for a bacterial toxin and represents the identification of a mechanism of action, cell-surface proteolytic activity, for a bacterial toxin. (elsevier.com)
To further expand and validate these in vitro biochemical studies, immunofluorescent histochemistry was performed, which colocalized N-cadherin and β-catenin to the same site of Sertoli-Sertoli and Sertoli-germ cell AJs, possibly ectoplasmic specializations near the basal compartment, at the lower third of the seminiferous epithelium in vivo as well as between Sertoli cells cultured in vitro. (popcouncil.org)
A stromal cell line also expressed N-cadherin in accordance with its fibroblastic morphology. (elsevier.com)
N-cadherin localized to areas of cell-cell contact in all cells that expressed it. (elsevier.com)
in contrast, E-cadherin expressing cell lines did not co-aggregate with stromal cells. (elsevier.com)
Lateral "cis" interfaces between cadherins from the same cell surface have been proposed to play a role in cadherin clustering. (elsevier.com)
L cell transfected with the mouse R-cadherin cDNA acquired a cadherin-mediated cell-cell adhesiveness as found for other cadherins. (houstonmethodist.org)
Auditory cortex interneuron development requires cadherins operating hair-cell mechanoelectrical transduction. (ifm-institute.org)
In fact, in adults, the majority of embryos showed the proper distribution of E-cadherin just beneath the membrane surfaces of all blastomeres and the percentage of embryos with this distribution increased with the increase in cell number during development. (uniss.it)
type I) are very similar in sequence and in structure yet the small differences between them are adequate to operate a vehicle cell patterning behavior 12 13 Including the difference in the binding affinities between N- and E-cadherin can be on the purchase of just one 1 kcal.mol-1 12. (scienceofgreathealth.com)
Furthermore, P-cadherin appearance was positive in every mucinous carcinomas and carcinosarcomas an undeniable fact that factors to a basal/myoepithelial cell histogenesis origins or type of differentiation of the tumour types [16,60,61], since P-cadherin is certainly a well-recognized biomarker of basal mammary carcinomas in human beings [6,62-65]. (clarkfrancis.com)
Abrogation of E-cadherin-mediated cell-cell contact in mouse embryonic stem cells results in reversible LIF-independent self-renewal. (ox.ac.uk)
We have previously demonstrated that differentiation of embryonic stem (ES) cells is associated with downregulation of cell surface E-cadherin. (ox.ac.uk)
In this study, we assessed the function of E-cadherin in mouse ES cell pluripotency and differentiation. (ox.ac.uk)
what does the type of cadherins involved dictate concerning cell-cell anchoring junctions? (flashcardmachine.com)
Cadherins are glycoproteins involved in Ca2+-mediated cell-cell adhesion. (embl.de)
Solution structure of the epithelial cadherin domain responsible for selective cell adhesion. (embl.de)
Glycosyltransferase POMGNT1 deficiency strengthens N-cadherin-mediated cell-cell adhesion. (mpg.de)

We conclude that high intracellular PKCK2activity confers anoikis resistance on esophageal cancer cells by inducing E- to N-cadherin switching. (elsevier.com)

In T-cadherin-deficient mice, APN failed to associate with cardiac tissue, and its levels dramatically increased in the circulation. (nih.gov)
Lymphocytes extracted from rejecting renal tissue are known to express the α E β 7 -integrin (CD103), a receptor for E-cadherin expressed on epithelial cells. (lww.com)
The capacity of CD103+ T cells to bind E-cadherin on tubular epithelial cells may be an important factor in the pathogenesis of specific tissue damage observed in acute renal allograft rejection. (lww.com)
There are multiple classes of cadherin molecule, each designated with a prefix (generally noting the type of tissue with which it is associated). (blogspot.com)
Objectives: To study Methylation in the promoter region of p15 and E Cadherin genes from tissue samples of head & neck cancer and compare it with peripheral blood. (journalcra.com)
In lactating or pseudo-lactating mammary tissue, P-cadherin immunoreactivity was observed in the cytoplasm of luminal epithelial cells and in luminal secretion, in line with data from previous studies in human breast [58] and canine mammary gland [59]. (clarkfrancis.com)

The E-cadherin staining was limited to the membrane of the epithelial layer. (elsevier.com)
Further investigation using this system resulted in a model whereby cadherins are constantly and spontaneously recruited to the plasma membrane , form lateral catenin-dependent associations and are subsequently released from these clusters in an active, ATP-dependent manner. (mechanobio.info)
Additionally, immunofluorescence data demonstrated that cells lacking PIPKIγ lost E-cadherin at the plasma membrane. (elsevier.com)
Wagoner, MP , Ling, K & Anderson, RA 2008, Tracking the transport of E-cadherin to and from the plasma membrane . (elsevier.com)

In light of this, several groups have proposed that in carcinomas, E-cadherin functions as an invasion suppressor molecule such that its loss permits or enhances the invasion of adjacent normal tissues. (biomedcentral.com)
We sought to determine the prevalence and nature of E-cadherin alterations in 'sporadic' gastric carcinomas. (unisi.it)
Moreover, P-cadherin positive carcinomas had an eightfold likelihood of developing neoplastic emboli than unfavorable tumours. (clarkfrancis.com)
Conclusions The full total outcomes demonstrate a romantic relationship between P-cadherin appearance and intense natural behavior of feline mammary carcinomas, recommending that P-cadherin may be regarded an indicator of poor prognosis within this pet species. (clarkfrancis.com)
Further analysis with follow-up research in feline types should be executed to be able to measure the prognostic worth of P-cadherin appearance in E-cadherin positive carcinomas. (clarkfrancis.com)
Nevertheless, the association between P-cadherin appearance as well as the histological kind of carcinomas had not been achieved in today's study, is not yet confirmed in human beings [5] and isn't consensual in various other pet models such as for example canines [16,56]. (clarkfrancis.com)

Then cells were intracellularly stained with purified 2.5 µg/ml of CD324 (E-Cadherin, clone DECMA-1) in blocking buffer overnight at 4°C followed by 2.5 µg/ml of DyLight™ 594 Goat anti-rat IgG (minimal x-reactivity) incubation for 1 hour at 4°C. Nuclei were counterstained with DAPI and are shown in blue. (biolegend.com)

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