A foul-smelling diamine formed by bacterial decarboxylation of lysine.
Organic chemicals which have two amino groups in an aliphatic chain.
A toxic diamine formed by putrefaction from the decarboxylation of arginine and ornithine.
Curved bacteria, usually crescent-shaped rods, with ends often tapered, occurring singly, in pairs, or short chains. They are non-encapsulated, non-sporing, motile, and ferment glucose. Selenomonas are found mainly in the human buccal cavity, the rumen of herbivores, and the cecum of pigs and several rodents. (From Bergey's Manual of Determinative Bacteriology, 9th ed)
A polyamine formed from putrescine. It is found in almost all tissues in association with nucleic acids. It is found as a cation at all pH values, and is thought to help stabilize some membranes and nucleic acid structures. It is a precursor of spermine.
A biogenic polyamine formed from spermidine. It is found in a wide variety of organisms and tissues and is an essential growth factor in some bacteria. It is found as a polycation at all pH values. Spermine is associated with nucleic acids, particularly in viruses, and is thought to stabilize the helical structure.
Enzymes that catalyze the addition of a carboxyl group to a compound (carboxylases) or the removal of a carboxyl group from a compound (decarboxylases). EC 4.1.1.
Compounds consisting of glucosamine and lactate joined by an ether linkage. They occur naturally as N-acetyl derivatives in peptidoglycan, the characteristic polysaccharide composing bacterial cell walls. (From Dorland, 28th ed)
An inhibitor of ORNITHINE DECARBOXYLASE, the rate limiting enzyme of the polyamine biosynthetic pathway.
An amino acid produced in the urea cycle by the splitting off of urea from arginine.
An enzyme that catalyzes the transfer of the propylamine moiety from 5'-deoxy-5'-S-(3-methylthiopropylamine)sulfonium adenosine to putrescine in the biosynthesis of spermidine. The enzyme has a molecular weight of approximately 73,000 kDa and is composed of two subunits of equal size.
A genus of gram-negative, anaerobic cocci parasitic in the mouth and in the intestinal and respiratory tracts of man and other animals.
A large group of anaerobic bacteria which show up as pink (negative) when treated by the Gram-staining method.
Decarboxylated arginine, isolated from several plant and animal sources, e.g., pollen, ergot, herring sperm, octopus muscle.
A pyridoxal-phosphate protein, believed to be the rate-limiting compound in the biosynthesis of polyamines. It catalyzes the decarboxylation of ornithine to form putrescine, which is then linked to a propylamine moiety of decarboxylated S-adenosylmethionine to form spermidine.
A species of halophilic bacteria in the genus VIBRIO, which lives in warm SEAWATER. It can cause infections in those who eat raw contaminated seafood or have open wounds exposed to seawater.
An essential amino acid. It is often added to animal feed.
Transglutaminases catalyze cross-linking of proteins at a GLUTAMINE in one chain with LYSINE in another chain. They include keratinocyte transglutaminase (TGM1 or TGK), tissue transglutaminase (TGM2 or TGC), plasma transglutaminase involved with coagulation (FACTOR XIII and FACTOR XIIIa), hair follicle transglutaminase, and prostate transglutaminase. Although structures differ, they share an active site (YGQCW) and strict CALCIUM dependence.
A group of compounds derived from ammonia by substituting organic radicals for the hydrogens. (From Grant & Hackh's Chemical Dictionary, 5th ed)
Membrane transporters that co-transport two or more dissimilar molecules in the opposite direction across a membrane. Usually the transport of one ion or molecule is against its electrochemical gradient and is "powered" by the movement of another ion or molecule with its electrochemical gradient.
A family of Urodela consisting of 15 living genera and about 42 species and occurring in North America, Europe, Asia, and North Africa.
Chemicals used in agriculture. These include pesticides, fumigants, fertilizers, plant hormones, steroids, antibiotics, mycotoxins, etc.
Lists of words, usually in alphabetical order, giving information about form, pronunciation, etymology, grammar, and meaning.
The study of the physical and chemical properties of a drug and its dosage form as related to the onset, duration, and intensity of its action.
Drugs intended for human or veterinary use, presented in their finished dosage form. Included here are materials used in the preparation and/or formulation of the finished dosage form.
Biogenic amines having more than one amine group. These are long-chain aliphatic compounds that contain multiple amino and/or imino groups. Because of the linear arrangement of positive charge on these molecules, polyamines bind electrostatically to ribosomes, DNA, and RNA.
The thick, yellowish-white, viscid fluid secretion of male reproductive organs discharged upon ejaculation. In addition to reproductive organ secretions, it contains SPERMATOZOA and their nutrient plasma.
A plant genus of the family CELASTRACEAE that is a source of triterpenoids and diterpene epoxides such as triptolide.
Literary and oral genre expressing meaning via symbolism and following formal or informal patterns.
A plant genus of the family GERANIACEAE. Geranium is also used as a common name for PELARGONIUM.
Publications printed and distributed daily, weekly, or at some other regular and usually short interval, containing news, articles of opinion (as editorials and letters), features, advertising, and announcements of current interest. (Webster's 3d ed)
INSECTS of the order Coleoptera, containing over 350,000 species in 150 families. They possess hard bodies and their mouthparts are adapted for chewing.
A segment of the LOWER GASTROINTESTINAL TRACT that includes the CECUM; the COLON; and the RECTUM.
A family of hexahydropyridines.
Amino acids with uncharged R groups or side chains.
A species of gram-positive, asporogenous, non-pathogenic, soil bacteria that produces GLUTAMIC ACID.
The aggregate enterprise of manufacturing and technically producing chemicals. (From Random House Unabridged Dictionary, 2d ed)
A genus of asporogenous bacteria that is widely distributed in nature. Its organisms appear as straight to slightly curved rods and are known to be human and animal parasites and pathogens.
Polymers where the main polymer chain comprises recurring amide groups. These compounds are generally formed from combinations of diamines, diacids, and amino acids and yield fibers, sheeting, or extruded forms used in textiles, gels, filters, sutures, contact lenses, and other biomaterials.
Exclusive legal rights or privileges applied to inventions, plants, etc.
A novel composition, device, or process, independently conceived de novo or derived from a pre-existing model.
Body of knowledge related to the use of organisms, cells or cell-derived constituents for the purpose of developing products which are technically, scientifically and clinically useful. Alteration of biologic function at the molecular level (i.e., GENETIC ENGINEERING) is a central focus; laboratory methods used include TRANSFECTION and CLONING technologies, sequence and structure analysis algorithms, computer databases, and gene and protein structure function analysis and prediction.
Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.
The syrup remaining after sugar is crystallized out of SUGARCANE or sugar beet juice. It is also used in ANIMAL FEED, and in a fermented form, is used to make industrial ETHYL ALCOHOL and ALCOHOLIC BEVERAGES.

Distinct sensitivities of OmpF and PhoE porins to charged modulators. (1/290)

The inhibition of the anion-selective PhoE porin by ATP and of the cation-selective OmpF porin by polyamines has been previously documented. In the present study, we have extended the comparison of the inhibitor-porin pairs by investigating the effect of anions (ATP and aspartate) and positively charged polyamines (spermine and cadaverine) on both OmpF and PhoE with the patch-clamp technique, and by comparing directly the gating kinetics of the channels modulated by their respective substrates. The novel findings reported here are (1) that the activity of PhoE is completely unaffected by polyamines, and (2) that the kinetic changes induced by ATP on PhoE or polyamines on OmpF suggest different mechanisms of inhibition. ATP induces a high degree of flickering in the PhoE-mediated current and appears to behave as a blocker of ion flow during its presumed transport through PhoE. Polyamines modulate the kinetics of openings and closings of OmpF, in addition to promoting a blocker-like flickering activity. The strong correlation between sensitivity to inhibitors and ion selectivity suggests that some common molecular determinants are involved in these two properties and is in agreement with the hypothesis that polyamines bind inside the pore of cationic porins.  (+info)

Inhibition of receptor internalization by monodansylcadaverine selectively blocks p55 tumor necrosis factor receptor death domain signaling. (2/290)

The 55-kDa receptor for tumor necrosis factor (TR55) triggers multiple signaling cascades initiated by adapter proteins like TRADD and FAN. By use of the primary amine monodansylcadaverine (MDC), we addressed the functional role of tumor necrosis factor (TNF) receptor internalization for intracellular signal distribution. We show that MDC does not prevent the interaction of the p55 TNF receptor (TR55) with FAN and TRADD. Furthermore, the activation of plasmamembrane-associated neutral sphingomyelinase activation as well as the stimulation of proline-directed protein kinases were not affected in MDC-treated cells. In contrast, activation of signaling enzymes that are linked to the "death domain" of TR55, like acid sphingomyelinase and c-Jun-N-terminal protein kinase as well as TNF signaling of apoptosis in U937 and L929 cells, are blocked in the presence of MDC. The results of our study suggest a role of TR55 internalization for the activation of select TR55 death domain signaling pathways including those leading to apoptosis.  (+info)

Inducing effect of diamines on transcription of the cephamycin C genes from the lat and pcbAB promoters in Nocardia lactamdurans. (3/290)

The diamines putrescine, cadaverine, and diaminopropane stimulate cephamycin biosynthesis in Nocardia lactamdurans, in shake flasks and fermentors, without altering cell growth. Intracellular levels of the P7 protein (a component of the methoxylation system involved in cephamycin biosynthesis) were increased by diaminopropane, as shown by immunoblotting studies. Lysine-6-aminotransferase and piperideine-6-carboxylate dehydrogenase activities involved in biosynthesis of the alpha-aminoadipic acid precursor were also greatly stimulated. The diamine stimulatory effect is exerted at the transcriptional level, as shown by low-resolution S1 protection studies. The transcript corresponding to the pcbAB gene and to a lesser extent also the lat transcript were significantly increased in diaminopropane-supplemented cultures, whereas transcription from the cefD promoter was not affected. Coupling of the lat and pcbAB promoters to the reporter xylE gene showed that expression from the lat and pcbAB promoters was increased by addition of diaminopropane in Streptomyces lividans. Intracellular accumulation of diamines in Nocardia may be a signal to trigger antibiotic production.  (+info)

Ehrlichia chaffeensis and E. sennetsu, but not the human granulocytic ehrlichiosis agent, colocalize with transferrin receptor and up-regulate transferrin receptor mRNA by activating iron-responsive protein 1. (4/290)

Ehrlichia chaffeensis and E. sennetsu are genetically divergent obligatory intracellular bacteria of human monocytes and macrophages, and the human granulocytic ehrlichiosis (HGE) agent is an obligatory intracellular bacterium of granulocytes. Infection with both E. chaffeensis and E. sennetsu, but not HGE agent, in the acute monocytic leukemia cell line THP-1 almost completely inhibited by treatment with deferoxamine, a cell-permeable iron chelator. Transferrin receptors (TfRs) accumulated on both E. chaffeensis and E. sennetsu, but not HGE agent, inclusions in THP-1 cells or the cells of the promyelocytic leukemia cell line HL-60. Reverse transcription-PCR showed an increase in the level of TfR mRNA 6 h postinfection which peaked at 24 h postinfection with both E. chaffeensis and E. sennetsu infection in THP-1 or HL-60 cells. In contrast, HGE agent in THP-1 or HL-60 cells induced no increase in TfR mRNA levels. Heat treatment of E. chaffeensis or the addition of monodansylcadaverine, a transglutaminase inhibitor, 3 h prior to infection inhibited the up-regulation of TfR mRNA. The addition of oxytetracycline 6 h after E. chaffeensis infection caused a decrease in TfR mRNA which returned to the basal level by 24 h postinfection. These results indicate that both internalization and continuous proliferation of ehrlichial organisms or the production of ehrlichial proteins are required for the up-regulation of TfR mRNA. Results of electrophoretic mobility shift assays showed that both E. chaffeensis and E. sennetsu infection increased the binding activity of iron-responsive protein 1 (IRP-1) to the iron-responsive element at 6 h postinfection and remained elevated at 24 h postinfection. However, HGE agent infection had no effect on IRP-1 binding activity. This result suggests that activation of IRP-1 and subsequent stabilization of TfR mRNA comprise the mechanism of TfR mRNA up-regulation by E. chaffeensis and E. sennetsu infection.  (+info)

Mechanisms of internalization of Staphylococcus aureus by cultured human osteoblasts. (5/290)

Staphylococcus aureus is an important bone pathogen, and evidence shows that this organism is internalized by chick osteoblasts. Here we report that S. aureus is internalized by human osteoblasts. Internalization was inhibited by monodansylcadaverine and cytochalasin D and to a lesser extent by ouabain, monensin, colchicine, and nocodazole. We propose that internalization occurs via a receptor-mediated pathway, requiring the participation of cytoskeletal elements, principally actin.  (+info)

Divalent cation-, nucleotide-, and polymerization-dependent changes in the conformation of subdomain 2 of actin. (6/290)

Conformational changes in subdomain 2 of actin were investigated using fluorescence probes dansyl cadaverine (DC) or dansyl ethylenediamine (DED) covalently attached to Gln41. Examination of changes in the fluorescence emission spectra as a function of time during Ca2+/Mg2+ and ATP/ADP exchange at the high-affinity site for divalent cation-nucleotide complex in G-actin confirmed a profound influence of the type of nucleotide but failed to detect a significant cation-dependent difference in the environment of Gln41. No significant difference between Ca- and Mg-actin was also seen in the magnitude of the fluorescence changes resulting from the polymerization of these two actin forms. Evidence is presented that earlier reported cation-dependent differences in the conformation of the loop 38-52 may be related to time-dependent changes in the conformation of subdomain 2 in DED- or DC-labeled G-actin, accelerated by substitution of Mg2+ for Ca2+ in CaATP-G-actin and, in particular, by conversion of MgATP- into MgADP-G-actin. These spontaneous changes are associated with a denaturation-driven release of the bound nucleotide that is promoted by two effects of DED or DC labeling: lowered affinity of actin for nucleotide and acceleration of ATP hydrolysis on MgATP-G-actin that converts it into a less stable MgADP form. Evidence is presented that the changes in the environment of Gln41 accompanying actin polymerization result in part from the release of Pi after the hydrolysis of ATP on the polymer. A similarity of this change to that accompanying replacement of the bound ATP with ADP in G-actin is discussed.  (+info)

Thymosin beta(4) serves as a glutaminyl substrate of transglutaminase. Labeling with fluorescent dansylcadaverine does not abolish interaction with G-actin. (7/290)

Thymosin beta(4) possesses actin-sequestering activity and, like transglutaminases, is supposed to be involved in cellular events like angiogenesis, blood coagulation, apoptosis and wound healing. Thymosin beta(4) serves as a specific glutaminyl substrate for transglutaminase and can be fluorescently labeled with dansylcadaverine. Two (Gln-23 and Gln-36) of the three glutamine residues were mainly involved in the transglutaminase reaction, while the third glutaminyl residue (Gln-39) was derivatized with a low efficiency. Labeled derivatives were able to inhibit polymerization of G-actin and could be cross-linked to G-actin by 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide. Fluorescently labeled thymosin beta(4) may serve as a useful tool for further investigations in cell biology. Thymosin beta(4) could provide a specific glutaminyl substrate for transglutaminase in vivo, because of the fast reaction observed in vitro occurring at thymosin beta(4) concentrations which are found inside cells. Taking these data together, it is tempting to speculate that thymosin beta(4) may serve as a glutaminyl substrate for transglutaminases in vivo and play an important role in transglutaminase-related processes.  (+info)

Covalent linkage of polyamines to peptidoglycan in Anaerovibrio lipolytica. (8/290)

Spermidine and cadaverine were found to be constituents of the cell wall peptidoglycan of Anaerovibrio lipolytica, a strictly anaerobic bacterium. The peptidoglycan was degraded with the N-acetylmuramyl-L-alanine amidase and endopeptidase into two peptide fragments, peptide I and peptide II, at a molar ratio of 4:1. Peptides I and II were identified as L-alanine-D-glutamic acid(alphacadaverine)gammameso-diaminopimelic acid (DAP)-D-alanine and L-alanine-D-glutamic acid(alphaspermidine)gammameso-DAP-D-alanine, respectively. The N(1)-amino group of spermidine was linked to the alpha-carboxyl group of the D-glutamic acid residue of peptide II.  (+info)

In vitro tests using mice found that piperidine formed from cadaverine in the large intestine; it was assumed to be created from cadaverine by intestinal bacteria.[2] No formation of piperidine from cadaverine was detected in the brain.[2]. ...
Limitation of natural sources, especially of fossil resources, for base material that is currently used to produce polyamides and related composites together with the increasing demand of these products, promotes the search for renewable sources of the base material. Fermentation by genetically engineered bacteria gains increasing interest as one of these possible sources. Cadaverine is a biogenic amine that can be produced by Corynebacterium glutamicum from the amino acid lysine by heterologous expression of a lysine decarboxylase from Escherichia coli. Overexpression of the patA and patD genes from Escherichia coli in Corynebacterium glutamicum enables the latter to further metabolize cadaverine to 5 aminovalerate (5AVA), which is a potential base material for the production of nylon 5 and a C5 platform for the synthesis of base materials for other polyamides. Commercial Opportunities Chemical industry is facing an increasing demand on polyamides and related composites, whereas in contrast the ...
TY - JOUR. T1 - Histamine, Putrescine and Cadaverine Formation in Spanish Semipreserved Anchovies as Affected by Time/Temperature. AU - RODRIGUEZ‐JEREZ, J. J.. AU - LOPEZ‐SABATER, E. I.. AU - HERNANDEZ‐HERRERO, M. M.. AU - MORA‐VENTURA, M. T.. PY - 1994/1/1. Y1 - 1994/1/1. N2 - We studied the changes in counts of mesophilic, psychrotrophic, entera‐bacteria, sulphite‐reducing and Vibrio microorganisms in commercial samples of Spanish semipreserved anchovies. The influence of NaCl, oxygen concentration and pH on bacterial growth and histamine, putrescine and cadaverine formation were also studied. Notable histamine formation was detected in samples incubated at 20°C and preserved in olive oil (443.68 ppm‐3012.13 ppm). This accumulation was probably caused by the conditions of the product, pH 5-6, relatively low NaCl (, 15%), and permissive temperature (product not refrigerated). Correlation between the microorganisms and histamine formation was not clear. Histamine was produced by ...
I just found out that my semen has cadaverine and putrescine . Cadaverine is a foul-smelling compound produced during putrefaction of animal tissue. The two compounds are largely responsible for the foul odor of putrefying flesh . The two compounds are also responsible for the smell and flavor of semen.. Please move to the appropriate section. Whichever that may be ...
Two genes,LAT1andOCT1, are likely to be involved in polyamine transport in Arabidopsis. Endogenous spermine levels modulate their expression and determine the sensitivity to cadaverine. Arabidopsis sp
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Chemical Entities of Biological Interest (ChEBI) is a freely available dictionary of molecular entities focused on small chemical compounds.
I think like you said he may have been to the apartment for the reason you said and seen the 3 children as he decribes. Happy and content and in a state where he felt that him and kate could slip off for their fun together. I cant see that if the children werent in a contented state that they would have slipped off to another room for some fun time. The child could have interrupted them which made her angry and most likely him angry also and he left. Then as you said she started to jump up and down on the chair shouting what she had seen. Thats when it happened and possibly then with shock and trying to control the other children she didnt immediately reach for the child. She may even have feared for the safety of the other 2. Maybe she has an uncontrollable anger issue that leaves her in such a state that it took a while for the anormaty of she done to register. He may have been returning to the tennis court when he or returning to see if she was ok after initially storming out after the ...
1,3-Diaminopentane 589-37-7 NMR spectrum, 1,3-Diaminopentane H-NMR spectral analysis, 1,3-Diaminopentane C-NMR spectral analysis ect.
H. Seidel-Rüfer, OW8. Brewery waste; country of origin unknown. Taxonomy/description (3896). Murein: A31, alpha-carboxyl group of glutamic acid is substituted by cadaverine (3896). (Medium 58 + NBB concentrate, 28°C, anaerobic ...
A list of 11 letter words that begin with C in the enable uncensored word list. cabbageworm cabinetries cabinetwork cachinnated cachinnates cacodemonic cacomistles cacophonies cacophonous cadastrally cadaverines caddishness caddisworms cafetoriums caffeinated cageynesses cajolements cakewalkers cakewalking calamanders etc.
This patent search tool allows you not only to search the PCT database of about 2 million International Applications but also the worldwide patent collections. This search facility features: flexible search syntax; automatic word stemming and relevance ranking; as well as graphical results.
The invention relates to recombinant microorganisms in which polynucleotides which code for lysine decarboxylase are enhanced and, using which, cadaverine (1,5-diaminopentane) is produced fermentatively, with the carbon source used preferably being renewable raw materials such as, for example, glucose, sucrose, molasses and the like.
Autophagy is a normal degradative pathway that involves the sequestration of cytoplasmic components and organelles in a vacuole called an autophagosome that finally fuses with the lysosome. Rab7 is a member of the Rab family involved in transport to late endosomes and in the biogenesis of the perinuclear lysosome compartment. To assess the role of Rab7 in autophagy we stably transfected CHO cells with wild-type pEGFP-Rab7, and the mutants T22N (GDP form) and Q67L (GTP form). Autophagy was induced by amino acid starvation and the autophagic vacuoles were labeled with monodansylcadaverine. By fluorescence microscopy we observed that Rab7wt and the active mutant Rab7Q67L were associated with ring-shaped vesicles labeled with monodansylcadaverine indicating that these Rab proteins associate with the membrane of autophagic vesicles. As expected, in cells transfected with the negative mutant Rab7T22N the protein was diffusely distributed in the cytosol. However, upon induction of autophagy by amino ...
The polyamines spermine, spermidine, putrescine, cadaverine, etc. have been implicated in a variety of cellular functions. However, details of their mode of interaction with other ubiquitous biomolecules is not known. We have solved a few structures of polyamine-amino acid complexes to understand the nature and mode of their interactions. Here we report the structure of a complex of putrescine with DL-glutamic acid. Comparison of the structure with the structure of putrescine-L-glutamic acid complex reveals the high degree of similarity in the mode of interaction in the two complexes. Despite the presence of a centre of symmetry in the present case, the arrangement of molecules is strikingly similar to the L-glutamic acid complex.. ...
The epidermal growth factor receptor (EGFR) has been shown to be a valid therapeutic target in breast cancer. This provides a strong rationale for the use of gefitinib, an inhibitor of the EGFR tyrosine kinase, in treatment of EGFR expressing breast cancers. Herein, we demonstrate a novel effect of gefitinib which involves induction of macroautophagy (autophagy) in breast cancer cells. Using imaging-based High Content Analysis (HCA) techniques we show that in MFC7 cells stably transfected with autophagy marker MAP1LC3 tagged with GFP (LC3GFP) (MCF7-LC3GFP), gefitinib causes relocation of cytoplasmic LC3GFP protein to autophagosomes resulting in accumulation of LC3GFP-positive vesicles in a concentration and time dependent fashion. The HCA data also revealed that the induction of autophagosomes in gefitinib treated MCF7-LC3GFP cells was accompanied by the parallel increase in the number of cytoplasmic vesicles which stained positively with acidotropic lysotracker red and monodansylcadaverine ...
Also in human (MCF-7 and MDA-MD-231) mammary tumor cells lines, γ-tocotrienol induced apoptosis through induction of autophagy with evidences of the presence of relatively large increase in the accumulation of monodansylcadaverine (MDC)-labeled vacuoles, a marker of autophagosome formation(9). In neoplastic(gene modification) +SA(high malignance) mammary epithelial cells, treatment with 4 microM gamma-tocotrienol, a dose that inhibited +SA cell growth by more than 50% compared with that of untreated control cells, decreased intracellular levels of activated PI3K/Akt (anti-apoptosis and increased cell proliferation) pathway(10). On mouse (+SA) and human (MCF-7, and MDA-MB-231) mammary cancer cell lines, Combined γ-tocotrienol and SU11274 (Met inhibitor) treatment resulted in synergistic inhibition through reduction in Akt (multiple cellular processes) STAT1/5 (activator of transcription 1,5 )and NFκB(a transcription factor that has crucial roles in inflammation, immunity, cell proliferation ...
Biogenic amines (BAs) are organic amines present in meat, fish, dairy produce and wine due to the breakdown of amino acids, catalysed by microbial decarboxylases. BAs determination in food is important not only because of possible toxicological effects such as nausea, sweating and headache but also due to their possible role as indicators of food spoilage. Chromatographic methods are traditionally applied for determination of BAs in food [1], which usually require preliminary operations for sample pre-treatment that are laborious and difficult to automate. On the other hand, screening analytical systems provide simple, low cost and rapid analysis with the possibility of subjecting high number of samples to the screening system in a short time [2]. In this work we present a novel method for screening determination of BAs using a microfluidic system with the detection by highly sensitive thermal lens microscope (μFIA-TLM). Four biogenic amines (putrescine, cadaverine, histamine and tyramine) were ...
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TY - JOUR. T1 - Tissue transglutaminase and the progression of human renal scarring. AU - Johnson, Timothy S.. AU - El-Koraie, Ahmed F.. AU - Skill, N.. AU - Baddour, Nahed M.. AU - El Nahas, A. Meguid. AU - Njloma, Melvin. AU - Adam, Ahmed G.. AU - Griffin, Martin. PY - 2003/8/1. Y1 - 2003/8/1. N2 - Experimental renal scarring indicates that tissue transglutaminase (tTg) may be associated with the accumulation of extracellular matrix (ECM), both indirectly via TGF-β1 activation and directly by the formation of ε(γ-glutamyl) lysine dipeptide bonds within the ECM. The latter potentially accelerates deposition and confers the ECM with resistance to proteolytic digestion. Studied were 136 human renal biopsy samples from a range of chronic renal diseases (CRD) to determine changes in tTg and ε(γ-glutamyl) lysine crosslinking. Immunofluorescence for insoluble tTg showed a 14-fold increase in the kidneys of CRD patients (5.3 ± 0.5 versus 76 ± 54 mV/cm2), which was shown to be active by a ...
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sp:TGM3_MOUSE] Tgm3, AI893889, TG_E, TG(E), TGE, TGase_E, TGase-3, we; transglutaminase 3, E polypeptide; K05620 transglutaminase 3 [EC:2.3.2.13] ...
sp:TGM3_MOUSE] Tgm3, AI893889, TG_E, TG(E), TGE, TGase_E, TGase-3, we; transglutaminase 3, E polypeptide; K05620 transglutaminase 3 [EC:2.3.2.13] ...
xtr:100145475 K05620 transglutaminase 3 [EC:2.3.2.13] , (RefSeq) tgm6; transglutaminase 6 (A) MRSDPQVIACDWYYAVNEKAHHTDVYESDDLVLRRGQPFKLTLMLNRPLQAEENILFIFE TGPSPSETSKTKVVFPLFRAENLQSWGAILTSIKSTSITVTINSHSDAVIGRYILSVAVQ CRGSDKPLPHQIGALNLLFNPWLQGDAVFMAEEDQRQEYVMNEQGVIFQGSDEDITSINW EYNQFERNILDICLVILDRSQNYKKDPALDVSQRNDPLYVCRVLSAMLNSKGDDGVLEEN WSDDYINGASPSSWNGSMNILKSWYYSKFKPVKYGQCWVYAGMLCTVLRSLGIPTRVITH FNSGQDKNANLFIDLQYKSQGSRQNDQEDQLWNFHVWNEAYFKRRDLGKSYNGWQVIDST PLKRSDGVYQCGPAPLTAIKEGDINLNYDVKYMFASVNADVACWIYYRNGTKKQVSNNAK ETGKFISTKAIGSNDRVDVTNNYKYAKGSEKEIKVFEKALKLSKGSFVGRTREKPRGGNI LFGRCTLDQIATFGQDFNLILSLKNLTPDSINVTVYINTSAILYTGRQRHTIWTGGKFLS LGPNLEKRFSIPVKYGQYSKHMTDNGVICMTALCEVEGTEERILVERNVSLVKPPLSITL PDKAIINQESNANIVIVNPLLETLNSCILWVEGYGLTDKILKKEVPSLKPGQSSEHNFVI TPMKTGVRTLLVNFSCDKIQNMKGSGKILITYSQEVSDSPVPT ...
In this study, the quantitative determination of eight biogenic amines (cadaverine, serotonin, histamine, spermidine, spermine, tyramine, putrescine and β-phenylethylamine) by an liquid chromatography method with evaporative light scattering detection was performed. The analysis of several samples of conventional, organic and fair trade cocoa-derivatives showed that organic and fair trade samples always contain much lower amine concentrations in comparison with their conventional counterparts, supporting the idea that biogenic amines can be regarded as cocoa quality markers. Irrespective of the kind of sample, results also showed that the most abundant amines were histamine, tyramine, spermidine, putrescine and spermine while β-phenylethylamine, cadaverine and serotonine have been found more rarely, all the amines never reaching dangerous amounts for consumer health. With the aim to confirm the experimental results, clustering analysis was performed on samples and instrumental results using principal
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TY - JOUR. T1 - Importance of tissue transglutaminase in repair of extracellular matrices and cell death of dermal fibroblasts after exposure to a solarium ultraviolet A source. AU - Gross, Stephane R.. AU - Balklava, Zita. AU - Griffin, Martin. PY - 2003/7/23. Y1 - 2003/7/23. N2 - Investigations were undertaken to study the role of the protein cross-linking enzyme tissue transglutaminase in changes associated with the extracellular matrix and in the cell death of human dermal fibroblasts following exposure to a solarium ultraviolet A source consisting of 98.8% ultraviolet A and 1.2% ultraviolet B. Exposure to nonlethal ultraviolet doses of 60 to 120 kJ per m2 resulted in increased tissue transglutaminase activity when measured either in cell homogenates, in situ by incorporation of fluorescein-cadaverine into the extracellular matrix or by changes in the epsilon(gamma-glutamyl) lysine cross-link. This increase in enzyme activity did not require de novo protein synthesis. Incorporation of ...
The microbial production of d-lysine has been of great interest as a medicinal raw material. Here, a two-step process for d-lysine production from l-lysine by the successive microbial racemization and asymmetric degradation with lysine racemase and decarboxylase was developed. The whole-cell activities of engineered Escherichia coli expressing racemases from the strains Proteus mirabilis (LYR) and Lactobacillus paracasei (AAR) were first investigated comparatively. When the strain BL21-LYR with higher racemization activity was employed, l-lysine was rapidly racemized to give dl-lysine, and the d-lysine yield was approximately 48% after 0.5 h. Next, l-lysine was selectively catabolized to generate cadaverine by lysine decarboxylase. The comparative analysis of the decarboxylation activities of resting whole cells, permeabilized cells, and crude enzyme revealed that the crude enzyme was the best biocatalyst for enantiopure d-lysine production. The reaction temperature, pH, metal ion additive, and
Bacillus methanolicus is a thermophilic methylotroph able to overproduce amino acids from methanol, a substrate not used for human or animal nutrition. Based on our previous RNA-seq analysis a mannitol inducible promoter and a putative mannitol activator gene mtlR were identified. The mannitol inducible promoter was applied for controlled gene expression using fluorescent reporter proteins and a flow cytometry analysis, and improved by changing the -35 promoter region and by co-expression of the mtlR regulator gene. For independent complementary gene expression control, the heterologous xylose-inducible system from B. megaterium was employed and a two-plasmid gene expression system was developed. Four different replicons for expression vectors were compared with respect to their copy number and stability. As an application example, methanol-based production of cadaverine was shown to be improved from 11.3 to 17.5 g/L when a heterologous lysine decarboxylase gene cadA was expressed from a ...
Agmatine, a divalent diamine with two positive charges at physiological pH, is transported into the matrix of liver mitochondria by an energy-dependent mechanism the driving force of which is ΔΨ (electrical membrane potential). Although this process showed strict electrophoretic behaviour, qualitatively similar to that of polyamines, agmatine is most probably transported by a specific uniporter. Shared transport with polyamines by means of their transporter is excluded, as divalent putrescine and cadaverine are ineffective in inhibiting agmatine uptake. Indeed, the use of the electroneutral transporter of basic amino acids can also be discarded as ornithine, arginine and lysine are completely ineffective at inducing the inhibition of agmatine uptake. The involvement of the monoamine transporter or the existence of a leak pathway are also unlikely. Flux-voltage analysis and the determination of activation enthalpy, which is dependent upon the valence of agmatine, are consistent with the ...
en] Soon after death, carcasses release volatile chemicals that attract carrion insects including Silphidae. Nevertheless, it is not known which chemical cues are involved in the attractiveness of the carcass. So far, little information is available on the chemical ecology of carrion beetles, particularly concerning the subfamily of Silphinae. The biological role of selected cadaveric volatile organic compounds including: dimethyldisulfide (DMDS), butan-1-ol, n-butanoic acid, indole, phenol, p-cresol, putrescine, and cadaverine on the silphine species, Thanatophilus sinuatus Fabricius, was investigated by using both electrophysiological and behavioural techniques. Among the tested cadaveric compounds, butan-1-ol and DMDS elicited the strongest EAG from both T. sinuatus male and female antennae. In a two-arm olfactometer, males and females were significantly attracted to dimethyldisulfide (DMDS) for both tested doses, whereas only males were attracted to p-cresol at 100 ng. Putrescine was ...
Fiore Sardo is a Protected Designation of Origin (PDO) cheese produced in Sardinia (Italy) from raw sheeps milk, presenting risk factors due to an accumulation of Biogenic Amines (BA). A total of 37 Fiore Sardo cheese samples produced in 19 dairy farms were collected from local retail stores to evaluate BA content and its relationship with free amino acids (FAA) and composition. The following were determined for each sample: pH, water activity, composition (moisture, dry matter, NaCl, protein and fat content). FAA and BA, after extraction, were determined by HPLC-FL. The total BA content in Fiore Sardo PDO cheese samples was 127±87 mg 100 g-1, ranging between 6 and 366 mg 100 g-1. Tyramine showed the highest concentration (82±51 mg 100 g-1), followed by putrescine (21±26 mg 100 g-1). Moreover, cadaverine, histamine, β-phenylethylamine and tryptamine were detected at concentrations lower than 10 mg 100 g-1. Overall 54% of the samples analysed exceeded the threshold of 90 mg 100 g-1 for total ...
Vaginal odor does change. It fluctuates naturally, and sometimes it can get worse. The most common cause of unpleasant changes to vaginal odor is bacterial vaginosis, which occurs when something upsets the balance between the beneficial lactobacilli bacteria that normally live in the vagina and pathogenic bacteria. What can upset the balance?. The vagina is supposed to be an acidic environment; thats how the healthy lactobacilli thrive. If something upsets that pH balance, tilting it toward alkalinity, unhealthy bacteria gain a foothold and become predominant, and begin producing unpleasant-smelling amines like putrescine, tyramine, and cadaverine. This is bacterial vaginosis. As it turns out, the lactobacilli bacteria normally present in the vagina are instrumental in maintaining an acidic pH. They consume glycogen, spit out lactic acid, and exert antimicrobial and antifungal effects that block common vaginal pathogens like candida, e. coli, and gardnerella from taking hold and causing ...
Free amino acids (FAAs) and biogenic amines were determined in whole hake (Merluccius merluccius L) stored in bulk under controlled or modified atmosphere (60% CO2:15% O2:25% N2) for 12 days at 2°C and followed by storage in trays either under the same special atmosphere or under air for 31 days at 2°C, which was the control. At the end of the storage, significant differences were observed (P≤0.05) in the amino acids isoleucine, leucine, methionine, phenylalanine, valine, glutamic acid and glycine between the control lot and the treated lots, and it was the control lot which exhibited the highest concentrations. However, the lots kept in special atmospheres exhibited higher levels of alanine, β-alanine, threonine, and tryptophan than the control lot. There was no relationship between the decreases in histidine, tyrosine, lysine, arginine and ornithine initial concentrations and the increases in their corresponding biogenic amine concentrations (histamine, tyramine, cadaverine, agmatine and ...
You are viewing an interactive 3D depiction of the molecule n-[4-({[(6r)-2-amino-5-methyl-4-oxo-3,4,5,6,7,8-hexahydro-6-pteridinyl]methyl}amino)benzoyl]-d-glutamic acid (C20H26N7O6+) from the PQR.
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The present invention relates to a DNA fragment having a gene derived from fish which codes for a polypeptide possessing transglutaminase activity, a recombinant plasmid comprising a fish-derived DNA fragment which codes for a transglutaminase, a transformant into which a recombinant plasmid comprising a fish-derived DNA fragment which codes for a transglutaminase is introduced, and a method for the production of a transglutaminase, comprising culturing a transformant containing a fish-derived DNA fragment which codes for a transglutaminase.
Tissue transglutaminase (tTG) belongs to the family of transglutaminase enzymes that catalyze the posttranslational modification of proteins via Ca(2+)-dependent cross-linking reactions. The catalytic action of tTG results in the formation of an isopeptide bond that is of great physiological significance since it is highly resistant to proteolysis and denaturants. Although tTG-mediated cross-linking reactions have been implicated to play a role in diverse biological processes, the precise physiological function of the enzyme remains unclear. Recent data, however, suggest that the protein polymers resulting from tTG-catalyzed reactions may play a role in commitment of cells to undergo apoptosis. On the same token, tTG-mediated formation of insoluble protein aggregates may underlie the markers of numerous pathological conditions, such as the senile plaques in Alzheimers disease and the Lewy bodies in Parkinsons disease. In addition to catalyzing Ca(2+)-dependent cross-linking reactions, tTG can also
The present invention relates to use of a transglutaminase in the preparation of a medicament for inhibiting angiogenesis. Preferably, the transglutaminase is a human tissue transglutaminase. Advantageously, the medicament is for treating cancer, rheumatoid arthritis, retinopathy and/or psoriasis. Additionally, the invention relates to compositions comprising a transglutaminase in an amount sufficient to inhibit angiogenesis.
A tissue transglutaminase (TTG) IgA and/or IgG test is used as part of an evaluation for certain autoimmune conditions, most notably celiac disease.
Required for Pcad induction, a promoter upstream of cadBA that is responsible for the pH-regulated expression of CadA and CadB. Probably acts as an activating transcription factor.
Transglutaminase type 2 (TG2) is a ubiquitously expressed person in the transglutaminase family members, with the capacity of mediating a transamidation response between a number of proteins substrates. to raise intracellular calcium mineral amounts. We demonstrate in live cells that inhibitors of TG2 transamidation activity can differentially impact the conformation from the enzyme. The irreversible inhibitor of TG2, NC9, pushes the enzyme into an open up conformation, whereas the reversible inhibitor CP4d traps TG2 in the shut conformation. Hence, this biosensor provides brand-new mechanistic insights in to the actions of two TG2 inhibitors and defines two brand-new classes predicated on capability to alter TG2 GBR-12909 conformation furthermore to inhibiting transamidation activity. Upcoming applications of the biosensor is to discover little molecules that particularly alter TG2 conformation to have an effect on GDP/GTP or calcium mineral binding. Launch Transglutaminase type 2 (TG2; EC ...
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Enzyme immunoassay for the detection of IgA antibodies to tissue transglutaminase (h-tTG) in human serum or plasma. SmartEIA kit is specifically designed for automated analysis using the Agility instrument.
1KV3: Structural basis for the guanine nucleotide-binding activity of tissue transglutaminase and its regulation of transamidation activity.
PROBIND CH is a functional formulation based on the Transglutaminase enzyme which is specially designed to improve the texture and final yield in cheese...
"IdeasTap Magazine - Editor Interview". Cadaverine Magazine. 22 June 2011. De Haan, Peter (9 March 2015). "The End of an Era". ...
Naerdal, Ingemar; Pfeifenschneider, Johannes; Brautaset, Trygve; Wendisch, Volker F. (2015). "Methanol-based cadaverine ... cadaverine and gamma-aminobutyric acid from methanol. The methylotrophic metabolism of B. methanolicus is being explored for ...
"Identifying human diamine sensors for death related putrescine and cadaverine molecules". PLOS Computational Biology. 14 (1): ...
Tracer studies using 13C-15N-doubly labeled cadaverine have shown three units of cadaverine are incorporated into sparteine and ... The deaminated cadaverine is not released from the enzyme, thus is can be assumed that the enzyme catalyzes the formation of ... The first intermediate in the biosynthesis is cadaverine, the decarboxylation product of lysine catalyzed by the enzyme lysine ... Enzymatic evidence then showed that the three molecules of cadaverine are transformed to the quinolizidine ring via enzyme ...
They are structurally and biosynthetically related to the diamines putrescine and cadaverine. Polyamine metabolism is regulated ...
In wine, histamine, cadaverine, phenylethylamine, putrescine, and tyramine have all been detected. These amines are created by ...
Nordenström, Björn E. W. (1951). "Effect of cadaverine and lysine on the urinary excretion of piperidine in rabbits". Acta ...
The substances cadaverine and putrescine are produced during the decomposition of animal (including human) bodies, and both ... a larger dose for cadaverine); there are no effects at all for a tenth of that dose. While figures for humans are not available ... putrescine and cadaverine in rats". Food and Chemical Toxicology. 35 (3-4): 337-348. doi:10.1016/S0278-6915(97)00121-X. ISSN ...
This cell can also use L-lysine, nitrate and cadaverine as sole nitrogen sources. This species cannot ferment. C. albidosimilis ...
CS1 maint: discouraged parameter (link) Interview with Sarah Rayner, Cadaverine Magazine, 22 February 2010. One Moment, One ...
... putrescine and cadaverine. The 1892 Merck's Bulletin stated, "We name such products of bacterial origin ptomaines; and the ... special alkaloid produced by the comma bacillus is variously named Cadaverine, Putrescine, etc." While The Lancet stated, "The ...
This species is also able to assimilate L-lysine, nitrate and cadaverine as nitrogen sources. Vishniac H. S., Kurtzman C. P. ( ...
... can also be compounded as a vinyl sulfone, with ethylenediamine, or with cadaverine.[clarification needed] ...
The disulfides than binds to DNA and precipitate nucleoproteins have an analogous interaction like cadaverine and spermidine ...
In the biosynthetic process, lysine is first decarboxylated into cadaverine, which is then oxidatively deaminated to the ...
They were founded soon after the disestablishment of Brisbane death metal act Cadaverine by three of its five members. Jordan ...
Review by Annabella Massey, Cadaverine Magazine Peter Bush's official website Works by or about Peter Bush in libraries ( ...
Stage 2: Putrefaction: Bacteria grow throughout the body, releasing gases, including cadaverine, which in turn bloat the body ...
... cadaverine, or spermidine) to the SpeF leader. Other non-coding RNAs uncovered in the same analysis include: suhB, ybhL, metA ...
... its body will begin to exude a foul odor caused by the presence of bacteria and the emission of cadaverine and putrescine. Some ...
... is able to use nitrate, nitrite and cadaverine, a protein created when animals decay and which produces ...
... a black precipitate Two common decarboxylation products of protein associated with decomposition are putrescine and cadaverine ...
... cadaverine (pentane-1,5-diamine) Cadaverine 6 carbons: hexamethylenediamine (hexane-1,6-diamine), trimethylhexamethylenediamine ...
... rotten meat Cadaverine, rotten meat Heterocyclic compounds Indole, smelling of feces Skatole, smelling of feces The US ...
Agmatine Cadaverine Putrescine Spermine Spermidine There is a distinction between endogenous and exogenous biogenic amines. ...
... phenylethylamine tyrosine to tyramine histidine to histamine serine to ethanolamine glutamic acid to GABA lysine to cadaverine ...
... such as putrescine and cadaverine, in its tissues. Los Alamos Scientific Laboratory STURP Radiocarbon 14 dating of the Shroud ...
... and cadaverine (from lysine), which carry the noxious odor of rotten flesh. Initially, the gases of putrefaction are ...
Brefeldin A Brassinolide Brucine Butyric acid Cadaverine Caffeine Calciferol (Vitamin D) Calcitonin Calmodulin Calreticulin ...
... and producing foul-smelling chemicals including putrescine and cadaverine. Cells in tissues break down and release hydrolytic ...
Cadaverine is a toxic diamine with the formula NH2(CH2)5NH2, which is similar to putrescines NH2(CH2)4NH2. Cadaverine is also ... Pentolinium and pentamethonium are both chemical derivatives of cadaverine. Cadaverine is toxic in large doses. In rats it has ... Elevated levels of cadaverine have been found in the urine of some patients with defects in lysine metabolism. The odor ... Cadaverine is the decarboxylation product of the amino acid lysine. However, this diamine is not purely associated with ...
... is a alkane-α,ω-diamine (CHEBI:35411) cadaverine (CHEBI:18127) is conjugate base of cadaverine(2+) ( ... cadaverine (CHEBI:18127). monodansylcadaverine (CHEBI:52007) has functional parent cadaverine (CHEBI:18127). cadaverine(2+) ( ... cadaverine (CHEBI:18127) has role Daphnia magna metabolite (CHEBI:83056) cadaverine (CHEBI:18127) has role Escherichia coli ... cadaverine (CHEBI:18127) has role mouse metabolite (CHEBI:75771) cadaverine (CHEBI:18127) has role plant metabolite (CHEBI: ...
... called cadaverine, are foul-smelling compounds found in decaying flesh. Amines are colourless; aliphatic amines are transparent ... and H2N(CH2)5NH2, called cadaverine, are foul-smelling compounds found in decaying flesh. Amines are colourless; aliphatic ...
Alexa Fluor 594 Cadaverine is useful as a polar tracer and as a reactive dye for labeling proteins via a carboxylic acid moiety ... Alexa Fluor® 594 Cadaverine is useful as a polar tracer and as a reactive dye for labeling proteins via a carboxylic acid ... Alexa Fluor® cadaverines can be used as reactive molecules for adding a fluorescent label to carboxylic acids using a coupling ... Alexa Fluor® cadaverines make excellent fluorescent polar tracers because they are bright, small, and water soluble. Since they ...
Previous research suggests cadaverine has a role in stress response, with groups reporting an increase in content upon exposure ... Previous research suggests cadaverine has a role in stress response, with groups reporting an increase in content upon exposure ... Cadaverine is also present in the environment; it can be produced by rhizosphere and phyllosphere microbes. Markedly, exogenous ... Cadaverine is also present in the environment; it can be produced by rhizosphere and phyllosphere microbes. Markedly, exogenous ...
Recombinant XBHaLDC produced a 1.3-fold higher titer of cadaverine (6.1 g/L) than the XBEcLDC strain (4.8 g/L) from 10 g/L of l ... Cadaverine is produced by the decarboxylation of l-lysine using a lysine decarboxylase (LDC). In this study, we developed ... Cadaverine synthesized via a whole cell biocatalyst reaction using XBHaLDC was purified to polymer grade, and purified ... Furthermore, XBHaLDC, concentrated to an optical density (OD600) of 50, efficiently produced 136 g/L of cadaverine from 200 g/L ...
4-Sulforhodamine 101 Cadaverine, Trifluoroacetate Salt 4-Sulforhodamine 101 cadaverine can be coupled to activated carboxy ... 4-Sulforhodamine 101 cadaverine can be coupled to activated carboxy groups or sulfonyl chlorides. ...
Endogenous spermine levels modulate their expression and determine the sensitivity to cadaverine. Arabidopsis sp ... Arabidopsis Cadaverine response L-Amino acid Transporter 1 Organic Cation Transporter 1 Polyamine Spermine ... Kuznetsov V, Shorina M, Aronova E, Stetsenko L, Rakitin V, Shevyakova N (2007) NaCl- and ethylene-dependent cadaverine ... Spermine modulates the expression of two probable polyamine transporter genes and determines growth responses to cadaverine in ...
Cadaverine and putrescine in semen I just found out that my semen has cadaverine and putrescine . Cadaverine is a foul-smelling ... Dont know about putrescine, but cadaverine is a natural ingredient of the semen. Its not a problem. If You want to taste like ...
Fluorescein-5-carboxamide cadaverine] from Creative Biomart. 5-FAM cadaverine [Fluorescein-5-carboxamide cadaverine] can be ... Fluorescein-5-carboxamide cadaverine] 5-FAM cadaverine [Fluorescein-5-carboxamide cadaverine]. 5-FAM cadaverine Related ... 5-FAM cadaverine is an excellent building block to prepare fluorescent ligands for receptor binding assays. Additionally, we ... See All 5-FAM cadaverine Products Bring this labeled protein directly to your bench! ...
Modeling and experimental verification of ligand-receptor interaction in a high affinity cadaverine receptor with an unusual ... Modeling and experimental verification of ligand-receptor interaction in a high affinity cadaverine receptor with an unusual ...
Cadaverine Magazine. The best new poetry, prose and non-fiction from emerging writers under 30 ...
"IdeasTap Magazine - Editor Interview". Cadaverine Magazine. 22 June 2011. De Haan, Peter (9 March 2015). "The End of an Era". ...
... it was assumed to be created from cadaverine by intestinal bacteria.[2] No formation of piperidine from cadaverine was detected ... Cadaverine is a diamine that occurs as an endogenous amine in humans. Its the decarboxylation product of the essential amino ... 2. Metabolism of cadaverine and pipecolic acid in brain and other organs of the mouse. ... In vitro tests using mice found that piperidine formed from cadaverine in the large intestine; ...
Production of carbon-13-labeled cadaverine by engineered Corynebacterium glutamicum using carbon-13-labeled methanol as co- ... Production of carbon-13-labeled cadaverine by engineered Corynebacterium glutamicum using carbon-13-labeled methanol as co- ... Production of carbon-13-labeled cadaverine by engineered Corynebacterium glutamicum using carbon-13-labeled methanol as co- ... L. Leßmeier, et al., "Production of carbon-13-labeled cadaverine by engineered Corynebacterium glutamicum using carbon-13- ...
Be the first to review "Cadaverine 1%" Cancel reply. Your email address will not be published. Required fields are marked * ... From the name you might expect Cadaverine to smell like a rotting corpse and while there is certainly an element of that, most ... Solvent: MMB (with 10% ethanol and 0.1% BHT) to form a 0.1% solution of Cadaverine ...
Though both the cadaverine molecules in the asymmetric unit have an all trans conformation, the carbon backbones are slightly ... The structure of cadaverine dihydrochloride monohydrate has been determined by X-ray crystallography with the following ... Between the concave surfaces of two bent cadaverine molecules exists water channels all along the short b axis. The water ... Crystal structure of cadaverine dihydrochloride monohydrate. Indian Journal of Biochemistry & Biophysics. 1992 Oct; 29(5): 402- ...
... cadaverine dihydrochloride chemical synthesis methods, cadaverine dihydrochloride synthetic routes ect. ... cadaverine dihydrochloride 1476-39-7 Synthesis RouteTotal: 4 Synthesis Route 65898-76-2. ~97%. 107-46-0. 1476-39-7. View Detail ...
Cadaverine is a biogenic amine that can be produced by Corynebacterium glutamicum from the amino acid lysine by heterologous ... the patA and patD genes from Escherichia coli in Corynebacterium glutamicum enables the latter to further metabolize cadaverine ...
... cadaverine (1,5-diaminopentane) is produced fermentatively, with the carbon source used preferably being renewable raw ... The production of cadaverine is aided by additionally overexpressing, in the cadaverine-producing recombinant cell, a ... Accumulation of cadaverine in the culture supernatant of recombinant strains of Corynebacterium glutamicum DM1800. C. ... Example 4 Cadaverine Production Using Bacteria The recombinant C. glutamicum DM1800 strains were grown at 30° C. overnight on ...
Cadaverine, Spermine and Spermidine - Enhanced Precatalyst Preparation Stabilization and Initiation (EPPSI) ... Putrescine and Cadaverine also contribute towards the smells of some living processes. Since they are both poisonous, the body ... Putrescine, Cadaverine, Spermine and Spermidine - Enhanced Precatalyst Preparation Stabilization and Initiation (EPPSI) →. Page ... Putrescine and Cadaverine also contribute to the distinctive smell of semen, which also contains the related molecules Spermine ...
Methanol-based cadaverine production by genetically engineered Bacillus methanolicus strains. Nærdal, Ingemar; Pfeifenschneider ... A volume-corrected concentration of 11.3 g l−1 of cadaverine was obtained by high-cell density fed-batch methanol fermentation ... Both CadA and LdcC were functional in B. methanolicus cultivated at 50°C and expression of cadA resulted in cadaverine ... By also investigating the cadaverine tolerance level, B. methanolicus proved to be an exciting alternative host and comparable ...
Hasne MP, Coppens I, Soysa R, Ullman B. A high-affinity putrescine-cadaverine transporter from Trypanosoma cruzi. Molecular ... Hasne, MP, Coppens, I, Soysa, R & Ullman, B 2010, A high-affinity putrescine-cadaverine transporter from Trypanosoma cruzi, ... A high-affinity putrescine-cadaverine transporter from Trypanosoma cruzi. In: Molecular Microbiology. 2010 ; Vol. 76, No. 1. pp ... Hasne, M. P., Coppens, I., Soysa, R., & Ullman, B. (2010). A high-affinity putrescine-cadaverine transporter from Trypanosoma ...
The engineered strain produced cadaverine from cellobiose more effectively than from glucose: 6.1 mM after 48 h from 28 g/L of ... we demonstrate the one-step production of cadaverine (1,5-diaminopentane) from cellobiose using an Escherichia coli strain ... Cadaverine fermentation from glucose. We then evaluated cadaverine production by Jm-cadA, Jm-blc-Tfu and Jm-cadA-blc-Tfu grown ... 2009). A cadaverine overproducing E. coli strain has been engineered by the introduction of the cadaverine biosynthesis, ...
Cadaverine dihydrochloride. 100 mg. DRE-C10933500. Add to basket Caffeic acid. 100 mg. DRE-C10934700. Add to basket ...
Cadaverine dihydrochloride. 100 mg. DRE-C10933500. Add to basket Caffeic acid. 100 mg. DRE-C10934700. Add to basket ...
Biogenic amines such as putrescine, cadaverine, and spermine are formed and degraded as a result of normal metabolic activity ...
Finally, we show in a small cross sectional study of 37 women that the BAs putrescine, cadaverine and tyramine are ... Finally, we show in a small cross sectional study of 37 women that the BAs putrescine, cadaverine and tyramine are ... and cadaverine and between both Mobiluncus spp. and Anaeroglobus spp. with putrescine. Macklaim et al. (2013) in their recent ... Putrescine and Cadaverine are Higher in CST IV Vaginal Microbiome. To determine the actual changes in vaginal BA levels ...
cadaverine (chemical compound). amine: Physical properties: and H2N(CH2)5NH2, called cadaverine, are foul-smelling compounds ...
  • Construction of an Escherichia coli strain unable to synthesize putrescine, spermidine, or cadaverine: characterization of two genes controlling lysine decarboxylase. (justia.com)
  • Parana Journal of Science and Education (PJSE) - v.4, n.5, (1-14) July 1, 2018 ISSN: 2447-6153 https://sites.google.com/site/pjsciencea 1 Putrescine, Cadaverine, Spermine and Spermidine - Enhanced Precatalyst Preparation Stabilization and Initiation (EPPSI) Nano Molecules Alireza Heidari1◊ and Ricardo Gobato2☼ 1 Faculty of Chemistry, California South University, 14731 Comet St. Irvine, CA 92604, USA. (readkong.com)
  • To cite this article: Alireza Heidari, Ricardo Gobato, Putrescine, Cadaverine, Spermine and Spermidine - Enhanced Precatalyst Preparation Stabilization and Initiation (EPPSI) Nano Molecules. (readkong.com)
  • Abstract In the current study, we study Putrescine, Cadaverine, Spermine and Spermidine-Enhanced Precatalyst Preparation Stabilization and Initiation (EPPSI) Nano molecules incorporation into the Nano Polymeric Matrix (NPM) by immersion of the Nano Polymeric Modified Electrode (NPME) as molecular enzymes and drug targets for human cancer cells, tissues and tumors treatment under synchrotron and synchrocyclotron radiations. (readkong.com)
  • Putrescine and Cadaverine also contribute to the distinctive smell of semen, which also contains the related molecules Spermine and Spermidine. (readkong.com)
  • Molecular structure of Putrescine, Cadaverine, Spermine and Spermidine Nano molecules. (readkong.com)
  • Overexpression of the TcPOT1.1 and TcPOT1.2 genes in T. cruzi epimastigotes revealed that TcPOT1.1 and TcPOT1.2 were high-affinity transporters that recognized both putrescine and cadaverine but not spermidine or spermine. (elsevier.com)
  • A pathoadaptation process involving the speG gene and the cad operon has led to spermidine accumulation and loss of cadaverine. (plos.org)
  • While a higher spermidine content promotes the survival of Shigella within infected macrophages, the lack of cadaverine boosts the pathogenic potential of the bacterium in host tissues. (plos.org)
  • Although many other BAs, except histamines such as putrescine, spermidine, methylamine, ethylamine, phenylethylamine, isoamylamine and cadaverine, are usually derived from the raw materials of grapes and grape must themselves, most of the BAs can be synthesized or degraded during fermentation. (scielo.br)
  • A) Microcalorimetric titrations of 15 µM TlpQ-LBD with 4.8 µl aliquots of 250 µM putrescine, spermidine, or cadaverine. (asm.org)
  • Basic amines such as putrescine, spermine, spermidine and cadaverine are responsible for the smell and flavor of semen. (biology-online.org)
  • Endogenous spermine levels modulate their expression and determine the sensitivity to cadaverine. (springer.com)
  • Arabidopsis spermine (Spm) synthase ( SPMS ) gene-deficient mutant was previously shown to be rather resistant to the diamine cadaverine (Cad). (springer.com)
  • All elapid venoms contained larger molar quantities of putrescine and cadaverine than spermine, but still at levels that are likely to be biologically insignificant. (mdpi.com)
  • The influence of NaCl, oxygen concentration and pH on bacterial growth and histamine, putrescine and cadaverine formation were also studied. (uab.cat)
  • MORA‐VENTURA, M. T. / Histamine, Putrescine and Cadaverine Formation in Spanish Semipreserved Anchovies as Affected by Time/Temperature . (uab.cat)
  • the optimal temperature was 25 °C. When we prepared and examined the BAs synthesis spectrum during the fermentation process, Bokbunja wine fermented with Saccharomyces cerevisiae showed that the histamine concentration increased from 2.72 of Bokbunja extract to 5.29 mg/L and cadaverine and dopamine was decreased to 2.6 and 10.12 mg/L, respectively. (scielo.br)
  • 8 It has been also demonstrated that many yeast strains present in wines ( Saccharomyces cerevisiae, Brettanomyces bruxellensis, Kloeckera apiculata , among others) can produce histamine, phenylethylamine and cadaverine. (scielo.br)
  • Cadaverine is a biogenic amine that can be produced by Corynebacterium glutamicum from the amino acid lysine by heterologous expression of a lysine decarboxylase from Escherichia coli. (provendis.info)
  • Overexpression of the patA and patD genes from Escherichia coli in Corynebacterium glutamicum enables the latter to further metabolize cadaverine to 5 aminovalerate (5AVA), which is a potential base material for the production of nylon 5 and a C5 platform for the synthesis of base materials for other polyamides. (provendis.info)
  • In the attempts to increase the synthesis of cadaverine, Escherichia coli strains are used which harbour a plasmid for over-expressing the homologous lysine decarboxylase (cadA). (justia.com)
  • In this study, we demonstrate the one-step production of cadaverine (1,5-diaminopentane) from cellobiose using an Escherichia coli strain displaying β-glucosidase (BGL) on its cell surface. (springeropen.com)
  • In the Gram-negative bacterium Escherichia coli , cadaverine is biosynthesized directly from L-lysine by two kinds of L-lysine decarboxylases: a constitutive one encoded by the ldcC gene, and an inducible one produced at low pH encoded by the cadA gene. (springeropen.com)
  • Our results demonstrated that efficient conversion of L-lysine into cadaverine presumably has severe effects on feedback regulation of the L-lysine biosynthetic pathway in B. methanolicus. (ntnu.no)
  • Finally, we show in a small cross sectional study of 37 women that the BAs putrescine, cadaverine and tyramine are significantly higher in CST IV over CSTs I and III. (frontiersin.org)
  • Cadaverine is a foul-smelling diamine compound produced by the putrefaction of animal tissue. (wikipedia.org)
  • Cadaverine is a toxic diamine with the formula NH2(CH2)5NH2, which is similar to putrescine's NH2(CH2)4NH2. (wikipedia.org)
  • Cadaverine is a C5 diamine monomer used for the production of bio-based polyamide 510. (mdpi.com)
  • Cadaverine is a diamine that occurs as an endogenous amine in humans. (wikidot.com)
  • The crystal is highly pseudo-symmetric with 2 molecules of cadaverine, 4 chloride ions and 2 partially disordered water molecules present in the asymmetric unit. (who.int)
  • Though both the cadaverine molecules in the asymmetric unit have an all trans conformation, the carbon backbones are slightly bent. (who.int)
  • Between the concave surfaces of two bent cadaverine molecules exists water channels all along the short b axis. (who.int)
  • For instance, the Leguminosae have been shown to produce cadaverine and use it as a precursor in the biosynthesis of quinolizidine alkaloids, secondary metabolites that are involved in insect defense and also display therapeutic pharmacological properties. (frontiersin.org)
  • The level of cadaverine in E. coli is regulated by biosynthesis, degradation, uptake, and export (Soksawatmaekhin et al. (springeropen.com)
  • 2009 ). A cadaverine overproducing E. coli strain has been engineered by the introduction of the cadaverine biosynthesis, degradation, and utilization pathways (Na et al. (springeropen.com)
  • IMSEAR at SEARO: Crystal structure of cadaverine dihydrochloride monohydrate. (who.int)
  • Ramaswamy S, Murthy MR. Crystal structure of cadaverine dihydrochloride monohydrate. (who.int)
  • 2011 ). This metabolically engineered E. coli strain could constitutively produce cadaverine to a high concentration in glucose mineral salts medium. (springeropen.com)
  • However, there are no reports of the use of E. coli strain to produce cadaverine using cellobiose as a carbon source. (springeropen.com)
  • Cadaverine is the decarboxylation product of the amino acid lysine. (wikipedia.org)
  • Cadaverine is produced by the decarboxylation of l -lysine using a lysine decarboxylase (LDC). (mdpi.com)
  • In zebrafish, the trace amine-associated receptor 13c (or TAAR13c) has been identified as a high-affinity receptor for cadaverine. (wikipedia.org)
  • Modeling and experimental verification of ligand-receptor interaction in a high affinity cadaverine receptor with an unusual bifunctional ligand requirement. (uni-koeln.de)
  • Alexa Fluor® 594 Cadaverine is useful as a polar tracer and as a reactive dye for labeling proteins via a carboxylic acid moiety. (thermofisher.com)
  • Alexa Fluor® cadaverines make excellent fluorescent polar tracers because they are bright, small, and water soluble. (thermofisher.com)
  • The amine-containing Alexa Fluor® cadaverines can also be used to label glutamine residues in some proteins and peptides via an enzyme-catalyzed transamidation reaction. (thermofisher.com)
  • Cadaverine, from the word, cadaver, is often associated with decaying matter and is one of the components that gives carrion its distinctive smell. (frontiersin.org)
  • From the name you might expect Cadaverine to smell like a rotting corpse and while there is certainly an element of that, most people are immediately struck by the overwhelming note of human sperm - at this dilution way, way stronger smelling than real ejaculate - which of course makes it ideal for those seeking to create perfumes with pheromones, sexuality and lust in mind. (pellwall.com)
  • It is usually accompanied by Cadaverine (named after the cadavers that give rise to it), a poisonous syrupy liquid with an equally disgusting smell. (readkong.com)
  • The odor commonly associated with bacterial vaginosis has been linked to cadaverine and putrescine. (wikipedia.org)
  • Despite a multitude of studies in bacterial systems, cadaverine has garnered little attention in plant research. (frontiersin.org)
  • By also investigating the cadaverine tolerance level, B. methanolicus proved to be an exciting alternative host and comparable to the well-known bacterial hosts E. coli and Corynebacterium glutamicum. (ntnu.no)
  • and H 2 N(CH 2 ) 5 NH 2 , called cadaverine, are foul-smelling compounds found in decaying flesh. (britannica.com)
  • Conjugation of some phenolic and cinnamic compounds with monodansyl cadaverine. (nii.ac.jp)
  • Monsanto's "Roundup-ready" corn has higher levels of putrescine and cadaverine, compounds responsible for dead body odor. (yudu.com)
  • Production of carbon-13-labeled cadaverine by engineered Corynebacterium glutamicum using carbon-13-labeled methanol as co-substrate", Applied Microbiology and Biotechnology , vol. 99, 2015, pp. 10163-10176. (uni-bielefeld.de)
  • In the present study, the methylotrophic and thermophilic bacterium Bacillus methanolicus was engineered into a microbial cell factory for the production of the platform chemical 1,5-diaminopentane (cadaverine) from methanol. (ntnu.no)
  • A volume-corrected concentration of 11.3 g l−1 of cadaverine was obtained by high-cell density fed-batch methanol fermentation. (ntnu.no)
  • This study represents the first demonstration of microbial production of cadaverine from methanol. (ntnu.no)
  • Interestingly, some women will complain of foul odor after intercourse, and the reaction is the same: semen is basic, and when it mixes with the discharge, you get the same release of cadaverine and putrescine. (grahamazon.com)
  • That characteristic odor of cadaverine and putrescine are unmistakable. (petmd.com)
  • The recombinant XBHaLDC and XBEcLDC strains were constructed for the high-level production of cadaverine. (mdpi.com)
  • Cadaverine derives from lysine in a pathway that is distinct from that of the other well-characterized ornithine- or arginine-derived polyamines. (frontiersin.org)
  • The invention relates to recombinant microorganisms in which polynucleotides which code for lysine decarboxylase are enhanced and, using which, cadaverine (1,5-diaminopentane) is produced fermentatively, with the carbon source used preferably being renewable raw materials such as, for example, glucose, sucrose, molasses and the like. (justia.com)
  • The invention relates to cadaverine-producing recombinant microorganisms with a high L-lysine titre, in which polynucleotides which code for lysine decarboxylase are present in an enhanced dose in comparison to microorganisms, which act as the parent strain, which are not modified with regard to this enzyme. (justia.com)
  • 2011 ). This high tolerance suggests that microorganisms can be metabolically engineered to overproduce cadaverine to industrially useful levels. (springeropen.com)
  • Recombinant XBHaLDC produced a 1.3-fold higher titer of cadaverine (6.1 g/L) than the XBEcLDC strain (4.8 g/L) from 10 g/L of l -lysine. (mdpi.com)
  • This E. coli strain produces increased amounts of cadaverine following the overexpression of the homologous cadA gene (JP 2002-223770). (justia.com)
  • We also address the possible roles rhizosphere and phyllosphere microbes may play in the delivery of exogenous cadaverine near plant organs, and discuss our current understanding of the molecular pathways that contribute to cadaverine homeostasis and response in plants. (frontiersin.org)
  • Cadaverine is at the nexus of several biochemical pathways (Figure 1 ). (frontiersin.org)
  • Elevated levels of cadaverine have been found in the urine of some patients with defects in lysine metabolism. (wikipedia.org)
  • 2. Metabolism of cadaverine and pipecolic acid in brain and other organs of the mouse. (wikidot.com)
  • It is a metabolite of cadaverine, a polyamine found in the human intestine. (ebi.ac.uk)
  • Don't know about putrescine, but cadaverine is a natural ingredient of the semen. (thundersplace.org)
  • Cadaverine is also known by the names 1,5-pentanediamine and pentamethylenediamine. (wikipedia.org)
  • A further method is the conversion of lysine-HCl into cadaverine by means of the isolated cadA enzyme (JP 2005-060447). (justia.com)
  • Salivary bacteria produce the enzyme lysine decarboxylase which converts lysine to cadaverine. (elsevier.com)
  • A selective and sensitive capillary electrophoresis method with laser-induced fluorescence detection has been developed for the determination of cadaverine and lysine in saliva, as an indicator of lysine decarboxylase enzyme activity. (elsevier.com)
  • 2004 ). It was recently shown that E. coli can tolerate a higher concentration of cadaverine, just slightly lower than that tolerated by a wild-type Corynebacterium glutamicum strain (Mimitsuka et al. (springeropen.com)
  • 5-FAM cadaverine is an excellent building block to prepare fluorescent ligands for receptor binding assays. (creativebiomart.net)
  • In humans, molecular modelling and docking experiments have shown that cadaverine fits into the binding pocket of the human TAAR6 and TAAR8. (wikipedia.org)
  • Pentolinium and pentamethonium are both chemical derivatives of cadaverine. (wikipedia.org)
  • Cadaverine functions in a multitude of cellular processes critical to living organisms. (frontiersin.org)
  • Furthermore, XBHaLDC, concentrated to an optical density (OD 600 ) of 50, efficiently produced 136 g/L of cadaverine from 200 g/L of l -lysine (97% molar yield) via an IPTG- and PLP-free whole cell bioconversion reaction. (mdpi.com)
  • Cadaverine synthesized via a whole cell biocatalyst reaction using XBHaLDC was purified to polymer grade, and purified cadaverine was successfully used for the synthesis of polyamide 510. (mdpi.com)
  • Polyamide 54 is made by the polycondensation of cadaverine and succinic acid. (springeropen.com)
  • Additionally, we have proven that the fluorescein cadaverine derivative is also a good transglutaminase substrate for site-specific protein labeling like FITC cadaverine. (creativebiomart.net)
  • Based on multivariate analyses using pre-debridement salivary metabolomics data, we found that metabolites associated with higher PISA included cadaverine and hydrocinnamate, while uric acid and ethanolamine were associated with lower PISA. (nih.gov)
  • Initially identified as a lysine decomposition product in organic matter, cadaverine, or 1,5-pentanediamine, is found ubiquitously in the environment. (frontiersin.org)
  • The resulting recombinant XBHaLDC strain was used as a whole cell biocatalyst for the high-level bioconversion of l -lysine into cadaverine without the supplementation of isopropyl β- d -1-thiogalactopyranoside (IPTG) for the induction of protein expression and pyridoxal phosphate (PLP), a key cofactor for an LDC reaction. (mdpi.com)
  • The engineered strain produced cadaverine from cellobiose more effectively than from glucose: 6.1 mM after 48 h from 28 g/L of consumed cellobiose, vs. 3.3 mM from 20 g/L of consumed glucose. (springeropen.com)
  • However, data regarding the role of cadaverine in stress response remains conflicted, as some plant systems show enhanced tolerance to stresses in its presence, while others show increased sensitivity to the same stresses. (frontiersin.org)
  • This may contribute to gingival inflammation, while cadaverine contributes to oral malodor. (elsevier.com)
  • The inventors have made it their object to provide novel methods for the fermentative production of cadaverine from renewable raw materials. (justia.com)
  • Both CadA and LdcC were functional in B. methanolicus cultivated at 50°C and expression of cadA resulted in cadaverine production levels up to 500 mg l−1 during shake flask conditions. (ntnu.no)
  • Cadaverine is particularly relevant in the production of bio-polyamides derived from renewable feedstocks as a replacement for conventional polyamides derived from petrochemicals. (springeropen.com)
  • Pathoadaption events affecting the cad locus have occurred also in EIEC, silencing cadaverine production. (plos.org)
  • Indeed, restoring cadaverine synthesis decreases the expression of the speC gene, whose product affects putrescine production. (plos.org)
  • In the context of this problem it has now been found that, starting from a lysine-producing microorganism, a cadaverine producer can be generated by introducing an optionally heterologous gene which codes for a lysine decarboxylase. (justia.com)
  • Smartphone-interrogated test supports for the enzymatic determination of putrescine and cadaverine in food. (springer.com)