Carbonic acid calcium salt (CaCO3). An odorless, tasteless powder or crystal that occurs in nature. It is used therapeutically as a phosphate buffer in hemodialysis patients and as a calcium supplement.
Human colonic ADENOCARCINOMA cells that are able to express differentiation features characteristic of mature intestinal cells, such as ENTEROCYTES. These cells are valuable in vitro tools for studies related to intestinal cell function and differentiation.
A group (or phylum) of unicellular EUKARYOTA (or algae) possessing CHLOROPLASTS and FLAGELLA.
A genus of pearl oysters in the family Pteriidae, class BIVALVIA. Both cultured and natural pearls are obtained from species in the genus. They are distinct from the distantly related, edible true oysters of the family OSTREIDAE.
The proteinaceous component of the pancreatic stone in patients with PANCREATITIS.
One of the largest orders of mostly marine CRUSTACEA, containing over 10,000 species. Like AMPHIPODA, the other large order in the superorder Peracarida, members are shrimp-like in appearance, have sessile compound eyes, and no carapace. But unlike Amphipoda, they possess abdominal pleopods (modified as gills) and their bodies are dorsoventrally flattened.
Process by which organic tissue becomes hardened by the physiologic deposit of calcium salts.
Lining of the INTESTINES, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. In the SMALL INTESTINE, the mucosa is characterized by a series of folds and abundance of absorptive cells (ENTEROCYTES) with MICROVILLI.
Salts or ions of the theoretical carbonic acid, containing the radical CO2(3-). Carbonates are readily decomposed by acids. The carbonates of the alkali metals are water-soluble; all others are insoluble. (From Grant & Hackh's Chemical Dictionary, 5th ed)
Tumors or cancer of the COLON.
Human colonic ADENOCARCINOMA cells that are able to express differentiation features characteristic of mature intestinal cells such as the GOBLET CELLS.
A great expanse of continuous bodies of salt water which together cover more than 70 percent of the earth's surface. Seas may be partially or entirely enclosed by land, and are smaller than the five oceans (Atlantic, Pacific, Indian, Arctic, and Antarctic).
A heterodimeric protein that is a cell surface antigen associated with lymphocyte activation. The initial characterization of this protein revealed one identifiable heavy chain (ANTIGENS, CD98 HEAVY CHAIN) and an indeterminate smaller light chain. It is now known that a variety of light chain subunits (ANTIGENS, CD98 LIGHT CHAINS) can dimerize with the heavy chain. Depending upon its light chain composition a diverse array of functions can be found for this protein. Functions include: type L amino acid transport, type y+L amino acid transport and regulation of cellular fusion.
The section of the alimentary canal from the STOMACH to the ANAL CANAL. It includes the LARGE INTESTINE and SMALL INTESTINE.
The salinated water of OCEANS AND SEAS that provides habitat for marine organisms.
Common name for two families of FLATFISHES belonging to the order Pleuronectiformes: left-eye flounders (Bothidae) and right-eye flounders (Pleuronectidae). The latter is more commonly used in research.
Substances that counteract or neutralize acidity of the GASTROINTESTINAL TRACT.
A semisynthetic cephalosporin antibiotic with antimicrobial activity similar to that of CEPHALORIDINE or CEPHALOTHIN, but somewhat less potent. It is effective against both gram-positive and gram-negative organisms.
Membrane transporters that co-transport two or more dissimilar molecules in the same direction across a membrane. Usually the transport of one ion or molecule is against its electrochemical gradient and is "powered" by the movement of another ion or molecule with its electrochemical gradient.
A class in the phylum CNIDARIA, comprised mostly of corals and anemones. All members occur only as polyps; the medusa stage is completely absent.
Calcium compounds used as food supplements or in food to supply the body with calcium. Dietary calcium is needed during growth for bone development and for maintenance of skeletal integrity later in life to prevent osteoporosis.
The gaseous envelope surrounding a planet or similar body. (From Random House Unabridged Dictionary, 2d ed)
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
A non-metal element that has the atomic symbol P, atomic number 15, and atomic weight 31. It is an essential element that takes part in a broad variety of biochemical reactions.
Animals that have no spinal column.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Community of tiny aquatic PLANTS and ANIMALS, and photosynthetic BACTERIA, that are either free-floating or suspended in the water, with little or no power of locomotion. They are divided into PHYTOPLANKTON and ZOOPLANKTON.
Uptake of substances through the lining of the INTESTINES.
A colorless, odorless gas that can be formed by the body and is necessary for the respiration cycle of plants and animals.
A polypeptide hormone (84 amino acid residues) secreted by the PARATHYROID GLANDS which performs the essential role of maintaining intracellular CALCIUM levels in the body. Parathyroid hormone increases intracellular calcium by promoting the release of CALCIUM from BONE, increases the intestinal absorption of calcium, increases the renal tubular reabsorption of calcium, and increases the renal excretion of phosphates.
Inorganic salts that contain the -HCO3 radical. They are an important factor in determining the pH of the blood and the concentration of bicarbonate ions is regulated by the kidney. Levels in the blood are an index of the alkali reserve or buffering capacity.
The extent to which the active ingredient of a drug dosage form becomes available at the site of drug action or in a biological medium believed to reflect accessibility to a site of action.
Inorganic salts of phosphoric acid.
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
Abnormally elevated PARATHYROID HORMONE secretion as a response to HYPOCALCEMIA. It is caused by chronic KIDNEY FAILURE or other abnormalities in the controls of bone and mineral metabolism, leading to various BONE DISEASES, such as RENAL OSTEODYSTROPHY.
Abnormally high level of calcium in the blood.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
A mass of organic or inorganic solid fragmented material, or the solid fragment itself, that comes from the weathering of rock and is carried by, suspended in, or dropped by air, water, or ice. It refers also to a mass that is accumulated by any other natural agent and that forms in layers on the earth's surface, such as sand, gravel, silt, mud, fill, or loess. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed, p1689)
The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Orientation of intracellular structures especially with respect to the apical and basolateral domains of the plasma membrane. Polarized cells must direct proteins from the Golgi apparatus to the appropriate domain since tight junctions prevent proteins from diffusing between the two domains.
Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The middle portion of the SMALL INTESTINE, between DUODENUM and ILEUM. It represents about 2/5 of the remaining portion of the small intestine below duodenum.
The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.
An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.
One or more layers of EPITHELIAL CELLS, supported by the basal lamina, which covers the inner or outer surfaces of the body.
A functional system which includes the organisms of a natural community together with their environment. (McGraw Hill Dictionary of Scientific and Technical Terms, 4th ed)
Established cell cultures that have the potential to propagate indefinitely.
A cell line derived from cultured tumor cells.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A metallic element that has the atomic symbol Mg, atomic number 12, and atomic weight 24.31. It is important for the activity of many enzymes, especially those involved in OXIDATIVE PHOSPHORYLATION.
A member of the alkali group of metals. It has the atomic symbol Na, atomic number 11, and atomic weight 23.
A specialized CONNECTIVE TISSUE that is the main constituent of the SKELETON. The principle cellular component of bone is comprised of OSTEOBLASTS; OSTEOCYTES; and OSTEOCLASTS, while FIBRILLAR COLLAGENS and hydroxyapatite crystals form the BONE MATRIX.
Tumors or cancer of the COLON or the RECTUM or both. Risk factors for colorectal cancer include chronic ULCERATIVE COLITIS; FAMILIAL POLYPOSIS COLI; exposure to ASBESTOS; and irradiation of the CERVIX UTERI.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A malignant epithelial tumor with a glandular organization.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Elements of limited time intervals, contributing to particular results or situations.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
The rate dynamics in chemical or physical systems.

Cell surface-associated lipoteichoic acid acts as an adhesion factor for attachment of Lactobacillus johnsonii La1 to human enterocyte-like Caco-2 cells. (1/3466)

The influence of pH on the adhesion of two Lactobacillus strains to Caco-2 human intestinal cells was investigated. One strain, Lactobacillus johnsonii La1, was adherent at any pH between 4 and 7. The other one, L. acidophilus La10, did not attach to this cell line under the same experimental conditions. On the basis of these results, we used the monoclonal antibody technique as a tool to determine differences on the surface of these bacteria and to identify a factor for adhesion. Mice were immunized with live La1, and the hybridomas produced by fusion of spleen cells with ONS1 cells were screened for the production of antibodies specific for L. johnsonii La1. A set of these monoclonal antibodies was directed against a nonproteinaceous component of the L. johnsonii La1 surface. It was identified as lipoteichoic acid (LTA). This molecule was isolated, chemically characterized, and tested in adhesion experiments in the same system. The adhesion of L. johnsonii La1 to Caco-2 cells was inhibited in a concentration-dependent way by purified LTA as well as by L. johnsonii La1 culture supernatant that contained LTA. These results showed that the mechanism of adhesion of L. johnsonii La1 to human Caco-2 cells involves LTA.  (+info)

Cytochrome P-450 1A1 expression in human small bowel: interindividual variation and inhibition by ketoconazole. (2/3466)

Human cytochrome P-450 1A1 (CYP1A1) is located primarily in extrahepatic tissues. To begin the characterization of this enzyme in the small intestine, we screened a bank of 18 human small intestinal microsomal preparations for CYP1A1 catalytic [(7-ethoxyresorufin O-deethylase (EROD)] activity and protein content. Although EROD activity was below detectable limits in 12 of the preparations, 6 exhibited measurable activity (1.4-123.5 pmol/min/mg), some exceeding that for 2 human liver microsomal preparations (11.0 and 26.4 pmol/min/mg). This variation was not due to variable quality of the preparations because each sample displayed readily detectable CYP3A4 catalytic activity and immunoreactive protein. We inadvertently found that intestinal EROD activity was inhibitable by ketoconazole at a concentration commonly believed to selectively inhibit CYP3A4. The possibility that CYP3A4 metabolizes 7-ethoxyresorufin was excluded because there was no correlation between intestinal CYP3A4 catalytic and EROD activity, and cDNA-expressed human CYP3A4 exhibited no EROD activity. Moreover, CYP1A1 immunoreactive protein was most abundant in the three intestinal preparations with the highest EROD activities, and the mean apparent Ki of ketoconazole observed for these three preparations (40 nM) was essentially identical with that for cDNA-expressed human CYP1A1 (37 nM). In summary, there is large interindividual variation in CYP1A1 expression in human small bowel, and ketoconazole is not a selective CYP3A4 inhibitor in in vitro metabolism studies involving intestinal tissue obtained from some individuals. These observations raise the possibility that in vivo drug interactions involving ketoconazole could result from CYP1A1 inhibition in the intestine in some individuals.  (+info)

CREB-binding [corrected] protein interacts with the homeodomain protein Cdx2 and enhances transcriptional activity. (3/3466)

Cdx2 encodes for a homeodomain protein that is expressed in intestinal epithelial cells. The Cdx2 protein triggers intestinal differentiation in cell lines and is necessary for maintenance of the intestinal phenotype in mice. CBP (cAMP response element-binding protein) is a transcriptional co-activator that interacts with many transcription factors and components of the basal transcriptional machinery. In this study, we demonstrate that CBP is markedly induced upon differentiation of the Caco-2 intestinal cell line and augments Cdx2-dependent transcriptional activity. Cdx2 interacts with the amino-terminal domain of CBP, and the two proteins coexist in vivo within the same nuclear protein complex. Moreover, expression of the CBP domain that interacts with Cdx2 acts as a dominant-negative inhibitor of transcriptional activation by Cdx2. These findings demonstrate a direct interaction between an intestinal homeodomain protein and CBP and suggest that CBP participates in the network of transcriptional proteins that direct intestinal differentiation.  (+info)

Regulation of the human apolipoprotein AIV gene expression in transgenic mice. (4/3466)

The apolipoprotein (Apo) AI-CIII-AIV gene cluster has a complex pattern of gene expression that is modulated by both gene- and cluster-specific cis-acting elements. In particular the regulation of Apo AIV expression has been previously studied in vivo and in vitro including several transgenic mouse lines but a complete, consistent picture of the tissue-specific controls is still missing. We have analysed the role of the Apo AIV 3' flanking sequences in the regulation of gene expression using both in vitro and in vivo systems including three lines of transgenic mice. The transgene consisted of a human fragment containing 7 kb of the 5' flanking region, the Apo AIV gene itself and 6 kb of the 3' flanking region (-7+6 Apo AIV). Accurate analysis of the Apo AIV mRNA levels using quantitative PCR and Northern blots showed that the 7+6 kb Apo AIV fragment confers liver-specific regulation in that the human Apo AIV transgene is expressed at approximately the same level as the endogenous mouse Apo AIV gene. In contrast, the intestinal regulation of the transgene did not follow, the pattern observed with the endogenous gene although it produced a much higher intestinal expression following the accepted human pattern. Therefore, this animal model provides an excellent substrate to design therapeutic protocols for those metabolic derangements that may benefit from variations in Apo AIV levels and its anti-atherogenic effect.  (+info)

Epidermal growth factor regulates fatty acid uptake and metabolism in Caco-2 cells. (5/3466)

Epidermal growth factor (EGF) has been reported to stimulate carbohydrate, amino acid, and electrolyte transport in the small intestine, but its effects on lipid transport are poorly documented. This study aimed to investigate EGF effects on fatty acid uptake and esterification in a human enterocyte cell line (Caco-2). EGF inhibited cell uptake of [14C]palmitate and markedly reduced its incorporation into triglycerides. In contrast, the incorporation in phospholipids was enhanced. To elucidate the mechanisms involved, key steps of lipid synthesis were investigated. The amount of intestinal fatty acid-binding protein (I-FABP), which is thought to be important for fatty acid absorption, and the activity of diacylglycerol acyltransferase (DGAT), an enzyme at the branch point of diacylglycerol utilization, were reduced. EGF effects on DGAT and on palmitate esterification occurred at 2-10 ng/ml, whereas effects on I-FABP and palmitate uptake occurred only at 10 ng/ml. This suggests that EGF inhibited palmitate uptake by reducing the I-FABP level and shifted its utilization from triglycerides to phospholipids by inhibiting DGAT. This increase in phospholipid synthesis might play a role in the restoration of enterocyte absorption function after intestinal mucosa injury.  (+info)

The isoflavone genistein inhibits internalization of enteric bacteria by cultured Caco-2 and HT-29 enterocytes. (6/3466)

The dietary isoflavone genistein is the focus of much research involving its role as a potential therapeutic agent in a variety of diseases, including cancer and heart disease. However, there is recent evidence that dietary genistein may also have an inhibitory effect on extraintestinal invasion of enteric bacteria. To study the effects of genistein on bacterial adherence and internalization by confluent enterocytes, Caco-2 and HT-29 enterocytes (cultivated for 15-18 d and 21-24 d, respectively) were pretreated for 1 h with 0, 30, 100, or 300 micromol/L genistein, followed by 1-h incubation with pure cultures of Listeria monocytogenes, Salmonella typhimurium, Proteus mirabilis, or Escherichia coli. Pretreatment of Caco-2 and HT-29 enterocytes with genistein inhibited bacterial internalization in a dose-dependent manner (r = 0.60-0.79). Compared to untreated enterocytes, 1-h pretreatment with 300 micromol/L genistein was generally associated with decreased bacterial internalization (P < 0. 05) without a corresponding decrease in bacterial adherence. Using Caco-2 cell cultures, decreased bacterial internalization was associated with increased integrity of enterocyte tight junctions [measured by increased transepithelial electrical resistance (TEER)], with alterations in the distribution of enterocyte perijunctional actin filaments (visualized by fluorescein-labeled phalloidin), and with abrogation of the decreased TEER associated with S. typhimurium and E. coli incubation with the enterocytes (P < 0.01). Thus, genistein was associated with inhibition of enterocyte internalization of enteric bacteria by a mechanism that might be related to the integrity of the enterocyte tight junctions, suggesting that genistein might function as a barrier-sustaining agent, inhibiting extraintestinal invasion of enteric bacteria.  (+info)

Transport of rhodamine 123, a P-glycoprotein substrate, across rat intestine and Caco-2 cell monolayers in the presence of cytochrome P-450 3A-related compounds. (7/3466)

Effects of cytochrome P-450 3A- and P-glycoprotein (P-gp)-related compounds, erythromycin, midazolam, ketoconazole, verapamil, and quinidine, on transport of rhodamine 123 (Rho-123), a P-gp substrate, were studied in rat intestine and in Caco-2 cells. Ileum was mainly used in rat studies because this segment showed greater P-gp-mediated Rho-123 transport. In an in vitro everted rat ileum, all the compounds examined significantly inhibited the transport of Rho-123 from serosal to mucosal surfaces across the intestine, with different inhibitory potencies among these compounds. In an in vivo rat study, the exsorption of Rho-123 from blood to the intestinal lumen, which was evaluated as exsorption clearance of Rho-123 under a steady-state plasma concentration of Rho-123, was also inhibited when these compounds were added to the intestinal lumen. Similarly, transepithelial transport of Rho-123 from the basolateral to apical side across Caco-2 cell monolayers was inhibited by these compounds. A linear relationship was observed in their inhibitory potencies on Rho-123 transport between in vitro and in vivo studies using rat ileum and between studies with rat ileum and Caco-2 cells. P-gp-mediated transport across the intestine was found to be inhibited not only by P-gp-related but also by all the cytochrome P-450 3A-related compounds examined. Within experimental error, the relative inhibitory potencies were the same between the studies with rat ileum (in vivo, in vitro) and those with Caco-2 cells. Thus, it is suggested that the function of P-gp and its sensitivity to these drugs may be similar in rat intestine and Caco-2 cells.  (+info)

Interactions of a nonpeptidic drug, valacyclovir, with the human intestinal peptide transporter (hPEPT1) expressed in a mammalian cell line. (8/3466)

The results of previous work performed in our laboratory using an in situ perfusion technique in rats and rabbit apical brush border membrane vesicles have suggested that the intestinal uptake of valacyclovir (VACV) appears to be mediated by multiple membrane transporters. Using these techniques, it is difficult to characterize the transport kinetics of VACV with each individual transporter in the presence of multiple known or unknown transporters. The purpose of this study was to characterize the interaction of VACV and the human intestinal peptide transporter using Chinese hamster ovary (CHO) cells that overexpress the human intestinal peptide transporter (hPEPT1) gene. VACV uptake was significantly greater in CHO cells transfected with hPEPT1 than in cells transfected with only the vector, pcDNA3. The optimum pH for VACV uptake was determined to occur at pH 7.5. Proton cotransport was not observed in hPEPT1/CHO cells, consistent with previously observed results in tissues and Caco-2 cells. VACV uptake was concentration dependent and saturable with a Michaelis-Menten constant and maximum velocity of 1.64 +/- 0.06 mM and 23.34 +/- 0.36 nmol/mg protein/5 min, respectively. A very similar Km value was obtained in hPEPT1/CHO cells and in rat and rabbit tissues and Caco-2 cells, suggesting that hPEPT1 dominates the intestinal transport properties of VACV in vitro. VACV uptake was markedly inhibited by various dipeptides and beta-lactam antibiotics, and Ki values of 12.8 +/- 2.7 and 9.1 +/- 1.2 mM were obtained for Gly-Sar and cefadroxil at pH 7.5, respectively. The present results demonstrate that VACV is a substrate for the human intestinal peptide transporter in hPEPT1/CHO cells and that although transport is pH dependent, proton cotransport is not apparent. Also, the results demonstrate that the hPEPT1/CHO cell system has use in investigating the transport kinetics of drugs with the human intestinal peptide transporter hPEPT1; however, the extrapolation of these transport properties to the in vivo situation requires further investigation.  (+info)

TY - JOUR. T1 - L-alanine absorption in human intestinal Caco-2 cells driven by the proton electrochemical gradient. AU - Thwaites, D T. AU - McEwan, Gordon T.A.. AU - Brown, C D. AU - Hirst, B H. AU - Simmons, N L. PY - 1994/6. Y1 - 1994/6. N2 - In human Caco-2 intestinal epithelial layers, L-alanine absorption can be energized by a proton gradient across the brush-border membrane. Acidification of the apical medium, even in Na(+)-free media, is associated with a saturable net transepithelial absorption of L-alanine. L-Alanine transport causes cytosolic acidification consistent with proton/amino acid symport. L-Alanine transport in Na(+)-free media is rheogenic, stimulating an inward short-circuit current in voltage-clamped epithelial monolayers. By measurement of rapid L-alanine influx across the apical membrane, L-alanine-stimulated inward short-circuit current and intracellular acidification in the same cell batch, we estimate L-alanine/proton stoichiometry to be 1:0.62 +/- 0.25 (SD) ...
Zinc absorption in the small intestine is one of the main mechanisms regulating the systemic homeostasis of this essential trace element. This review summarizes the key aspects of human zinc homeostasis and distribution. In particular, current knowledge on human intestinal zinc absorption and the influence of diet-derived factors on bioaccessibility and bioavailability as well as intrinsic luminal and basolateral factors with an impact on zinc uptake are discussed. Their investigation is increasingly performed using in vitro cellular intestinal models, which are continually being refined and keep gaining importance for studying zinc uptake and transport via the human intestinal epithelium. The vast majority of these models is based on the human intestinal cell line Caco-2 in combination with other relevant components of the intestinal epithelium, such as mucin-secreting goblet cells and in vitro digestion models, and applying improved compositions of apical and basolateral media to mimic the in ...
Sodium caprate (CIO), a medium chain fatty acid, is used clinically to enhance rectal absorption of the low molecular weight (MW) drug ampicillin. The main aim of this study was to investigate whether CIO also enhances the permeability of high MW model drugs in a model of the intestinal epithelium. The second aim was to present visual evidence of the route of enhanced transport across the epithelial cell layer. The studies were performed in Caco-2 monolayers cultured on permeable supports. The effects of non-toxic concentrations (≤ 13 mM) of CIO on drug transport across the monolayers was studied using monodisperse 14C-polyethylene glycols (MW 238-502; 14C-PEGs), 125I-Arg8-vasopressin (MW 1,208), 125I-insulin (MW 6,000) and FITC-labelled dextrans (MW 4,400 and 19,600; FD4 and FD20 respectively) as model drugs. Electron and confocal laser scanning microscopy were used to demonstrate transport routes across the epithelium. 10 mM C10 increased the permeability of all 14C-PEGs to approximately the ...
Note: DIPLOMES; GR - 53 332; Cornell University, Ithaca USA; . Consultable sur demande à la Bibliothèque de lEPFL / Offered in consultation at the EPFL library.. ...
TY - JOUR. T1 - Induction of differentiation of colon cancer cells by combined inhibition of kinases and histone deacetylase. AU - Lea, Michael A.. AU - Ibeh, Chinwe. AU - Shah, Neel. AU - Moyer, Mary P.. PY - 2007/3/1. Y1 - 2007/3/1. N2 - The MAP kinase pathway inhibitor U0126 in combination with butyrate promotes differentiation in some colon cancer cell lines. We examined several inhibitors of histone deacetylase (HDAC) in combination with U0126 and other protein kinase inhibitors to see if these effects are general properties of HDAC inhibitors or butyrate alone. Alkaline phosphatase and peptidase activities were examined as markers for cellular differentiation in the human colon cancer cell lines Caco-2 and HT29 and the minimally transformed NCM460. Several HDAC inhibitors caused greater increases of alkaline phosphatase in the cancer cells than in NCM460, in which butyrate was the only HDAC inhibitor that caused a consistent increase. Unlike the JNK and PKC inhibitors examined, the MEK 1/2 ...
A.J. Janecki, M.H. Montrose, P. Zimniak, A. Zweibaum, C.M. Tse, S. Khurana, M. Donowitz, Subcellular redistribution is involved in acute regulation of the brush border Na+/H+ exchanger isoform 3 in human colon adenocarcinoma cell line caco-2, J. Biol. Chem., 273 (1998) 8790-8798 ...
Permeability assay for predicting the in vivo absorption of drugs across the gut wall by measuring the rate of transport of a compound across the Caco-2 cell line. Caco 2 permeability assay is part of our portfolio of in vitro ADME screening services.. To understand the suitability of a test compound for oral dosing and to predict the absorption of orally-administered drugs, the Caco 2 assay is widely used across the pharmaceutical industry as an in vitro model of the human small intestinal mucosa.. The Caco-2 cell model mimics processes such as transcellular transport, paracellular transport, and some aspects of efflux and active transport. Caco-2 assay is used to predict human intestinal permeability and to investigate drug efflux.. Assessing transport in both directions (apical to basolateral and basolateral to apical) across the cell monolayer yields an efflux ratio.. Monolayers based on Caco-2 cells which express MDR1 and BCRP transporters allow the study of passive permeability, if the ...
Purpose. The aim of this study was to investigate transepithelial ionic permeation via the paracellular pathway of human Caco-2 epithelial monolayers and its contribution to absorption of the base guanidine. Methods. Confluent monolayers of Caco-2 epithelial cells were mounted in Ussing chambers and the transepithelial conductance and electrical potential difference (p.d.) determined after NaCl dilution or medium Na substitution (bi-ionic conditions). Guanidine absorption (J a-b) was measured ± transepithelial potential gradients using bi-ionic p.d.s. Results. Basal NaCl replacement with mannitol gives a transepithelial dilution p.d. of 28.0 ± 3.1 mV basal solution electropositive (P Cl/P Na = 0.34). Bi-ionic p.d.s (basal replacements) indicate a cation selectivity of NH4+ , K+∼Cs+ , Na+ , Li+ , tetraethylammonium+ , N-methyl-d- glucamine+∼choline+. Transepithelial conductances show good correspondence with bi-ionic potential data. Guanidine J a-b was markedly sensitive to imposed ...
When looking at Caco-2 cell cultures microscopically, it is evident even by visual inspection that the cells are heterogeneous. Over the years, the characteristics of the cells used in different laboratories around the world have diverged significantly, which makes it difficult to compare results across labs.[4] Despite such heterogeneity, Caco-2 cells have found applications in cell invasion studies, viral transfection research, and lipid transport.[5] Caco-2 cells are most commonly used not as individual cells, but as a confluent monolayer on a cell culture insert filter (e.g., Transwell). When cultured in this format, the cells differentiate to form a polarized epithelial cell monolayer that provides a physical and biochemical barrier to the passage of ions and small molecules.[3][6] The Caco-2 monolayer is widely used across the pharmaceutical industry as an in vitro model of the human small intestinal mucosa to predict the absorption of orally administered drugs with different kits such as ...
In order to evaluate the release of zonulin during the time of observation, the area under the curve (AUC) was calculated. All data are expressed as mean and SEM. Differences were considered significant at P < 0.05. A specific software package (SigmaStat for Windows version 3.00 SPSS Inc. San Jose, CA, USA) was used. Results Effects of gliadin and L.GG. treatments on Caco-2 monolayer barrier function (TER and lactulose flux) TER measurements were determined after the addition of viable L.GG, L.GG-HK and L.GG-CM to polarized monolayers of Caco-2 cells seeded on Transwell filter inserts. TER was measured before the addition of bacteria, at time zero (immediately after the bacteria administration) and then at various time intervals ranging from. 30 min to 6 h. A slight and not significant Selleck CHIR 99021 increase in TER was observed after 90 min from the addition of viable L.GG, as well as L.GG-HK and L.GG-CM to Caco-2 cells compared to control cells (data not shown). Addition of gliadin to the ...
hi friends ! iam handling few cell lines ! we recently bought Caco-2 cell line. i have ended up mixing caco-2 cells with hybridoma cells and now iam unable to remove this contamination. Hybridoma cell line has a doubling time of just 24hrs is growing fast and soon will replace Caco-2 cells. caco-2 cells are really slow growing cells. i tried few mwthods to isolate them but failed ...
Home / Equipment / BD BioCoat HTS Caco-2 Assay Systems Caco-2 Assay System Contains the BD BioCoat Fibrillar Collagen 24-Multiwell Insert System plus media to perform 24 individual three-day Caco-2 ...
Dietary non-heme iron contains ferrous [Fe(II)] and ferric [Fe(III)] iron fractions and the latter should hydrolyze, forming Fe(III) oxo-hydroxide particles, on passing from the acidic stomach to less acidic duodenum. Using conditions to mimic the in vivo hydrolytic environment we confirmed the formation of nanodisperse fine ferrihydrite-like particles. Synthetic analogues of these (~ 10 nm hydrodynamic diameter) were readily adherent to the cell membrane of differentiated Caco-2 cells and internalization was visualized using transmission electron microscopy. Moreover, Caco-2 exposure to these nanoparticles led to ferritin formation (i.e., iron utilization) by the cells, which, unlike for soluble forms of iron, was reduced (p=0.02) by inhibition of clathrin-mediated endocytosis. Simulated lysosomal digestion indicated that the nanoparticles are readily dissolved under mildly acidic conditions with the lysosomal ligand, citrate. This was confirmed in cell culture as monensin inhibited Caco-2 utilization
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La linea cellulare di adenocarcinoma del colon Caco-2, se coltivata a confluenza, differenzia spontaneamente verso un fenotipo intestinale. Poco è noto riguardo ai meccanismi che regolano il processo differenziativo di queste cellule che mostrano mutazioni inattivanti a livello delle proteine APC e β-catenina. Queste mutazioni sono solitamente associate ad una stimolazione costitutiva di geni regolativi del ciclo cellulare quali Myc e la ciclina D1. Nonostante ciò, le Caco-2 sono in grado di uscire dal ciclo cellulare e mostrano un controllo trascrizionale e traduzionale, regolato nel tempo, di geni specifici del differenziamento intestinale. Attraverso lo studio dellespressione e modulazione di RNA e di proteine nel processo differenziativo abbiamo notato che le proteine Myc e ciclina D1, entrambe bersaglio trascrizionale del complesso β-catenina/TCF, sono regolate in maniera differente. Abbiamo quindi deciso di indagare il ruolo della regolazione di Myc nel differenziamento delle Caco-2. ...
Consistent with recent observations (22), we found that Caco-2 cells did not express HGF but its receptor alone. The highest levels of c-Met (mRNA and protein) occurred 8-15 days after plating. HGF is known to stimulate the proliferation of most epithelial cells in vitro. In this study, we found that 10 ng/ml HGF exerted only a moderate mitogenic effect on Caco-2 cells, consistent with the results of other studies in which Caco-2 cells were incubated with the same concentration of HGF (22). This led to a modest shortening of the time required to reach confluence. The differentiation process therefore began earlier in the presence of HGF, and this may partly account for the large increase in expression of E-cadherin and villin proteins noted on immunoblots 4 days after plating. However, at that time, the magnitude of increase observed in the amounts of these two proteins in cells incubated with HGF compared with controls was much greater than that observed between cells before and after ...
The dynamic interactions of metabolism and secretion, and the role of transporters on drug absorption have been under considerable debate. The thorough understanding of intestinal processes would be learnt through modeling of intestinal data only in the absence of the contribution from the liver. Recent studies on the interactions between the P450s and Pgp in vitro have led to the conclusion that intestinal metabolism is enhanced by the secretory action of P-glycoprotein due to an increase in the mean residence time (MRT) of drug in the intestine (Benet and Cummins, 2001; Cummins et al., 2001; Johnson et al., 2001). Theoretical examinations on Caco-2 cells and intestinal vascular perfusion systems support the notion that the mean residence time was increased, but metabolism under linear conditions was in fact decreased in the presence of secretion, even though the mean residence time has increased; under nonlinear metabolism, instances exist whereby the rate of metabolite accrual may increase, ...
We have previously demonstrated that the 104-kDa LAP acts as an adhesion protein and that blocking with anti-LAP MAb-H7 caused a 50% reduction in WT binding to Caco-2 cells (36). LAP is mostly cytosolic, with only 10 to 15% expression on the cell surface (24), and expression is temperature, growth phase (40), and nutrient dependent (22). However, LAP when in contact with mammalian cells was not examined. Here scanning electron microscopy analysis indicated the expression of LAP when in contact with Caco-2 cells and uniform surface distribution (Fig. 1). Although no comparative study was made between in vitro and in vivo expression, the present study qualitatively determines expression in the WT but not in the mutant KB208 strain. It has been shown before that Clostridium difficile in contact with eukaryotic Vero cells also induced a two- to fivefold increase in expression of a GroEL adhesion molecule (20).. The present study was undertaken to identify the receptor for LAP in enterocyte-like ...
1. Chen, H., Attieh, Z. K., Dang, T., Huang, G., van der Hee, R. M., and Vulpe, C. Decreased hephaestin expression and activity leads to decreased iron efflux from differentiated Caco2 cells. J Cell Biochem, 107: 803-808, 2009. 2. Cosentino, S., Gravaghi, C., Donetti, E., Donida, B. M., Lombardi, G., Bedoni, M., Fiorilli, A., Tettamanti, G., and Ferraretto, A. Caseinphosphopeptide-induced calcium uptake in human intestinal cell lines HT-29 and Caco2 is correlated to cellular differentiation. J Nutr Biochem, 21: 247-254. 3. Mangian, H. F. and Tappenden, K. A. Butyrate increases GLUT2 mRNA abundance by initiating transcription in Caco2-BBe cells. JPEN J Parenter Enteral Nutr, 33: 607-617; discussion 617, 2009. 4. Petruzzelli, M., Groen, A. K., van Erpecum, K. J., Vrins, C., van der Velde, A. E., Portincasa, P., Palasciano, G., van Berge Henegouwen, G. P., Lo Sasso, G., Morgano, A., and Moschetta, A. Micellar lipid composition profoundly affects LXR-dependent cholesterol transport across CaCo2 ... is the marketplace for research antibodies. Find the right antibody for your research needs. Adherence potential of indigenous lactic acid bacterial isolates obtained from fermented foods of Western Himalayas to intestinal epithelial Caco-2 and HT-29 cell lines.
Free Online Library: Rosiglitazone Role in the Expression of KLF6 Caco-2 Colon Cancer Cells/Rol de Rosiglitazona en la Expresion de KLF6 en Celulas Caco-2 de Cancer de Colon. by International Journal of Morphology; Biological sciences Antibodies Research Cancer research Cell differentiation Colon cancer DNA binding proteins Fluorescence microscopy Oncology, Experimental Thiazolidinediones Viral antibodies
Caco-2 monolayers grown on permeable filters for 21 days in bicameral Transwell chambers were incubated with control medium, medium containing cytomix (CM), a cocktail containing the pro-inflammatory cytokines, IL-1β (1 μg/ml), TNF (10 ng/ml) and IFN-γ (1000 U/ml), or medium containing CM and one of these other pharmacologic agents: ethyl pyruvate (EP; H2O2 scavenger; 10 mM): PJ34 (PARP inhibitor; 5 μM): 3-aminobenzamide (3-AB; PARP inhibition; 3 μM): FeTPPS (ONOO- decomposition catalyst; 50 μM): C-PTIO (NO• scavenger; 100 μM): PDTC (NF-κB inhibitor; 100 μM) or L-NIL (selective iNOS inhibitor; 20 μM). Permeability was expressed as the apical-to-basolateral clearance (nLcm-1 h-1) of fluorescein-labelled dextran (FD4) during the last 48 hours of incubation. Expression of occludin and ZO-1 were assessed by Western blot and immunohistochemistry. ...
Human intestinal model from stem cells offers opportunity to study more complex contributors to human intestinal function and disease than was possible before.
The C2BBe1 (brush border expressing) cell line was cloned in 1988 from the Caco-2 cell line (ATCC® HTB-37™) by limiting dilution. The clone was selected on the basis of morphological homogeneity and exclusive apical villin localization.
Lactic acid decreases Fe(II) and Fe(III) retention but increases Fe(III) transepithelial transfer by Caco-2 cells. J Agric Food Chem. 2005 Aug 24; 53(17):6919-23 ...
ICK - ICK (untagged)-Kinase deficient mutant (K33M) of Human intestinal cell (MAK-like) kinase (ICK), transcript variant 2 available for purchase from OriGene - Your Gene Company.
Wampler, Jennifer L. et al Heat Shock Protein 60 Acts as a Receptor for the Listeria Adhesion Protein in Caco-2 Cells. Infection and Immunity 72.2 (2004): 931-936. Web. 06 Dec. 2019. ...
Publications, Species, Genomes and Genes, Scientific Experts, Research Topics about Biliary and Intestinal Cell Models of Drug Toxicity
Mods - I used search but the answer was not readily apparent in the fifty threads that came back I just have a couple quick questions so shove that...
TY - GEN. T1 - Characterizing A549 Cell Line as an Epithelial Cell Monolayer Model for Pharmacokinetic Applications. AU - Frost, Timothy S.. AU - Jiang, Linan. AU - Zohar, Yitshak. N1 - Funding Information: ACKNOWLEDGMENT This project was supported by the Arizona Biomedical Research Commission through grant ABRC ADHS14-082983, and by a NASA Space Grant Undergraduate Internship. Publisher Copyright: © 2018 IEEE.. PY - 2019/2/13. Y1 - 2019/2/13. N2 - Transport of three different molecules across a porous membrane, with and without a confluent A549 cell monolayer, has been investigated in Transwells and microfluidic devices. The A549 cell line was selected since it has extensively been utilized in toxicology studies due to its potential target for drug delivery of macro molecules. The measured molecular transport rate was found to decrease with increasing molecular size due to lower diffusivity. The confluent cell monolayer presents a barrier to molecular, significantly reducing the transport rate ...
Background & Aims: For optimal nutrient absorption to occur, the enterocyte must express a range of specialist ion-driven carrier proteins that function cooperatively in a linked and mutually dependent fashion. Thus, absorption via the human intestinal H+-coupled di/tripeptide transporter (hPepT1) is dependent on maintenance of the trans-apical driving force (the H+-electrochemical gradient) established, in part, by brush-border Na+/H+ exchanger (NHE3) activity. This study aimed to examine whether physiologic regulation of NHE3 activity can limit hPepT1 capacity and, therefore, protein absorption after a meal. Methods: hPepT1 and NHE3 activities were determined in intact human intestinal epithelial Caco-2 cell monolayers by measurements of [14C]glycylsarcosine transport and uptake, 22Na+-influx, H+-influx, and H+-efflux. Expression of NHE regulatory factors was determined by reverse-transcriptase polymerase chain reaction. Results: Optimal dipeptide transport was observed in the presence of a ...
Summary The pathogenesis of campylobacter enteritis is not well understood, but invasion into and translocation across intestinal epithelial cells may be involved in the disease process, as demonstrated for a number of other enteric pathogens. However, the mechanisms involved in these processes are not clearly defined for campylobacters. In this study, isolates were compared quantitatively in established assays with the enterocyte-like cell line, Caco-2, to determine the extent to which intracellular invasion contributes to translocation across epithelial cell monolayers, and whether isolates vary in this respect. Ten fresh Campylobacter isolates were compared and shown to differ in invasiveness by a factor of 10-fold by following their recovery from gentamicin-treated Caco-2 cells grown on non-permeable tissue-culture wells. Four of these isolates with contrasting invasive ability were also shown to vary in their ability to translocate across Caco-2 cells grown on semi-permeable Transwell inserts by a
Bovine colostrum is well known for its beneficial properties on health and development. It contains a wide variety of bioactive ingredients that are known to promote a number of cellular processes. Therefore the use of colostrum whey as a feed additive to promote intestinal health has been proposed, yet little is known about mechanisms implicated in its beneficial properties on intestinal epithelial cells. In the present paper, casein were removed from bovine colostrum and the remaining liquid, rich in bioactive compounds, was evaluated for its capacity to modulate cellular processes in porcine intestinal epithelial cell line IPEC-J2 and human colon adenocarcinoma cell line Caco-2/15. First, we verified the effect of colostrum whey and cheese whey on processes involved in intestinal wound healing, including cell proliferation, attachment, morphology and migration. Our results showed that colostrum whey promoted proliferation and migration, and decreased specifically the attachment of Caco-2/15 ...
The benzenearsonate, Roxarsone, has been used since 1944 as an antimicrobial, growth-promoting poultry feed additive. USGS and EPA report that Roxarsone (4-hydroxy-3-nitrobenzenearsonate) and metabolites, including AHBA (3-amino-4-hydroxybenzenearsonate), contaminate waterways at greater than 1100 tons annually. To assess human impact of these organic arsenic water contaminants, it was important to study their potential absorption. The human adenocarcinoma cell line, Caco-2, is a model for intestinal absorption. We found proliferative effects on Caco-2 cells at micromolar levels of these compounds, as monitored by [3H]-thymidine incorporation into DNA. Flow cytometry cell cycle analysis confirmed accumulation in S phase from 21% (control) to 36% (24 hour exposure to 10 μM AHBA). Confluent Caco-2 cells grown on collagen-coated Transwell plates were dosed on the apical side. After exposure, media from apical and basolateral sides were collected separately. Following removal of FBS by 30K centrifugal
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
In this study, using cAMP analogues, as well as the adenylate cyclase activator forskolin and the phosphodiesterase inhibitor, 3-isobutyl-1-methylxanthine, we have shown that cAMP potentiates cytokine induced iNOS activity, protein, and mRNA in the human intestinal epithelial cell line, DLD-1. Cyclic AMP has been shown to induce iNOS expression in many cell types but the majority of these are rat cell lines. Indeed, cAMP responsive element (CRE) sites have been identified in the rat iNOS gene39 and could be involved in the iNOS expression caused by cAMP alone or in combination with cytokines. Nevertheless, cAMP can also decrease iNOS expression in cells of rat origin.28 32 40 To our knowledge, only two studies have reported cAMP enhancement of iNOS expression in human cells, these being monocytes and T cells.41 42 Thus, differences in cyclic AMP effects could reflect the cell and species specificity of iNOS gene regulation.. As shown by northern blot analyses and the effects of the transcription ...
The aim of this study was to explore potential transport mechanisms of rutin deca (H-) sulfonate sodium (RDS) across Caco-2 cell monolayers. As an in-vitro model of human intestinal epithelial membrane, Caco-2 cells were utilized to evaluate the transepithelial transport characteristics of this hydrophilic macromolecular compound. Bi-directional transport study of RDS demonstrated that the apparent permeability (Papp) in the secretory direction was 1.4∼4.5-fold greater than the corresponding absorptive Papp at concentrations in the range 50.0∼2000 μm. The transport of RDS was shown to be concentration, temperature and pH dependent. In the presence of ciclosporin and verapamil, potent inhibitors of P-glycoprotein (P-gp)/MRP2, the absorptive transport was enhanced and secretory efflux was diminished. RDS significantly reduced the efflux ratio of the P-gp substrate rhodamine-123 in a fashion indicative of P-gp activity suppression, while rhodamine-123 competitively inhibited the polarized ...
Lipopolysaccharide causes an increase in intestinal tight junction permeability in vitro and in vivo by inducing enterocyte membrane expression and localization of TLR-4 and CD14
Glutamine supplementation has been advocated for patients requiring parenteral nutritional support. However, the direct effect of glutamine on neoplastic cells is poorly understood. We therefore investigated the effects of glutamine on the proliferation, differentiation, and cell-matrix interactions of two human colon carcinoma cell lines (Caco-2 and SW620) adapted to glutamine-free media. Doubling times were calculated by logarithmic transformation of serial cell counts. Alkaline phosphatase, cathepsin C (dipeptidyl peptidase), lactase, and isomaltase expression (markers of differentiation) were assayed by digestion of synthetic substrates. Adhesion to matrix proteins was assessed by colorimetric quantitation of toluidine blue staining of adherent cells. Surface expression of Caco-2 receptors for matrix proteins (integrins) was studied by biotinylation and immunoprecipitation with specific antibodies. Glutamine (1-10 mm) dose-dependently stimulated Caco-2 proliferation on all matrices studied ...
With the global population rising, the need for sustainable and resource-efficiently produced proteins with nutritional and health promoting qualities has become urgent. Proteins are important macronutrients and are involved in most, if not all, biological processes in the human body. This review discusses these absorption mechanisms in the small intestine. To study intestinal transport and predict bioavailability, cell lines are widely applied as screening models and often concern Caco-2, HT-29, HT-29/MTX and T84 cells. Here, we provide an overview of the presence and activities of peptide- and amino acid transporters in these cell models. Further, inter-laboratory differences are discussed as well as the culture micro-environment, both of which may influence cell culture phenotype and performance. Finally, the value of new developments in the field, including culturing cells in 3-dimensional systems under shear stress (i.e., gut-on-chips), is highlighted. In particular, their suitability in screening
Eicosanoids are lipid mediators synthesized by the arachidonic acid (AA) cascade from membrane polyunsaturated fatty acids (PUFA) trough three distinct pathways: cyclooxygenase, lipooxygenase (LOX) and cytochrome P450 pathways. Eicosapentaenoic acid, an n-3 PUFA, is also substrate of the enzymes of the AA cascade. Thus, 5-LOX gives rise to the 5-series leukotrienes, and 12-LOX generates 12-HEPE, the homologue of 12-HETE when the source is AA. Fish oil supplementation has been used as a preventive measure against a number of diseases including coronary heart disease, cancer, and inflammatory bowel disease (IBD). IBD and other intestinal diseases are associated with the disruption of epithelial barrier function( 1 ). In this sense, we recently demonstrated that PGE2, which is increased in the mucosa of IBD patients, induces the disruption of this barrier in differentiated Caco-2 cells( 2 ). In this line, the present study aims to investigate the potential of 12-LOX derived eicosanoids 12-HETE and ...
Richard E. Hartman, Robert B. W. Smith, Roberta S. Hartman, Charles E. Butterworth, Jack M. Molesworth; The Electron Microscopy of Human Intestinal Epithelium Obtained with the Crosby Intestinal Biopsy Capsule . J Biophys and Biochem Cytol 25 January 1959; 5 (1): 171-172. doi: Download citation file:. ...
1. The interaction of cyclosporin A (CyA) with p-glycoprotein during intestinal uptake was investigated by a combination of in vitro experiments with human Caco-2 cells and an intubation study in healthy volunteers. 2. CyA uptake into the cells was not saturable and exhibited only a low temperature sensitivity, suggesting passive diffusion. When the permeation of CyA across Caco-2 monolayers from the apical to the basolateral side was determined, overall transport had an apparently saturable component up to a concentration of 1 microM. At higher concentrations permeation increased over-proportionally. Calculation of the kinetic parameters of apical to basolateral permeation suggested a diffusional process with a KD of 0.5 microliter min-1 per filter, which was overlayed by an active system in basolateral to apical direction with a KM of 3.8 microM and a Jmax of 6.5 picomol min-1 per filter. 3. CyA permeation was significantly higher when the drug was given from the basolateral side as compared ...
Differentiation environment so as to provide enterocyte-like monolayer with barrier functions, thus physiologically mimicking the human intestinal epithelium.
Caco-2 assays measure the ability of a drug to be absorbed from the gastrointestinal tract and thereby to evaluate whether the drug can be suitably dosed via an oral route. It also provides information regarding the ability of a compound to serve as a substrate for the P-glycoprotein (Pgp) efflux drug pump.. Caco-2 cells are intestinal epithelial tumor cells that form a polarized monolayer, a well-defined brush border on the apical surface and intercellular junctions when plated on a semipermeable membrane in a Transwell® dish.. ...
Organic cations (OCs) are substances of endogenous (e. iodide) uptake in an enterocyte cell line (Caco-2). Caco-2 cells were subcultured with two different media conditions (physiological: 5 mM glucose, referred as control cells; and high-glucose: 25 mM glucose, referred as HG cells). In HG cells, the uptake was significantly lower than in control cells. Redox changing interventions affected Mpp+ uptake, both in control and in high-glucose Caco-2 cells. Cellular glutathione levels could have an important impact on membrane transporter activity. The full total results indicate that modifications in the cellular oxidative state modulate MPP+ uptake by Caco-2 cells. Such modifications might reflect in changes of nutritional and drug bioavailability. = 6). *p 0.05. Procyanidins had been fractionated by molecular pounds chromatographically, according with their structural difficulty (Desk 1).1 In todays function, all tested procyanidin fractions (FI-FV) increased 3-MPP+ uptake in both control and HG ...
BioAssay record AID 695938 submitted by ChEMBL: Apparent permeability across human Caco2 cells st 200 uM measured over 150 mins by LC/MS analysis.
in BMC Cell Biology (2006), 7. Background: The absorptive and goblet cells are the main cellular types encountered in the intestine epithelium. The cell lineage Caco-2 is a model commonly used to reproduce the features of the bowel ... [more ▼]. Background: The absorptive and goblet cells are the main cellular types encountered in the intestine epithelium. The cell lineage Caco-2 is a model commonly used to reproduce the features of the bowel epithelium. However, there is a strong debate regarding the value of Caco-2 cell culture to mimick in vivo situation. Indeed, some authors report in Caco-2 a low paracellular permeability and an ease of access of highly diffusible small molecules to the microvilli, due to an almost complete lack of mucus. The HT29-5M21 intestinal cell lineage is a mucin-secreting cellular population. A co-culture system carried out in a serum-free medium and comprising both Caco-2 and HT29-5M21 cells was developed. The systematic use of a co-culture system requires the ...
Using intestinal (Caco-2) cells, we found that oxidant-induced disruption of barrier integrity requires microtubule disassembly. Protein kinase C (PKC)-δ isoform seems to be essential for disruption, but the mechanism is unknown. Because inducible nitric-oxide synthase (iNOS) is key to oxidant stress, we hypothesized that PKC-δ activation is essential in oxidant-induced iNOS up-regulation and the consequent cytoskeletal oxidation and disarray and monolayer barrier dysfunction. Cells were transfected with an inducible plasmid to overexpress native PKC-δ or with a dominant-negative to inhibit the activity of native PKC-δ. Clones were then incubated with oxidant (H2O2) ± modulators. Parental cells were treated similarly. Exposure to oxidant-disrupted monolayers by increasing native PKC-δ activity, increasing six iNOS-related variables (iNOS activity and protein, nitric oxide, oxidative stress, tubulin oxidation and nitration), decreasing polymerized tubulin, disrupting the cytoarchitecture of ...
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TY - JOUR. T1 - Permeability and toxicity characteristics of L-cysteine and 2-methyl-thiazolidine-4-carboxylic acid in Caco-2 cells. AU - Kartal-Hodzic, Alma. AU - Marvola, Tuuli. AU - Schmitt, Mechthild. AU - Harju, Kirsi. AU - Peltoniemi, Marikki. AU - Siven, Mia PY - 2013/11. Y1 - 2013/11. KW - 317 Pharmacy. U2 - 10.3109/10837450.2012.659253. DO - 10.3109/10837450.2012.659253. M3 - Article. VL - 18. SP - 1288. EP - 1293. JO - Pharmaceutical Development and Technology. JF - Pharmaceutical Development and Technology. SN - 1083-7450. IS - 6. ER - ...
Cell proliferation, differentiation, and death are a continuous process in intestinal mucosa homeostasis. Although cancer cell lines, such as Caco-2 and HT29, may not completely replicate normal growth characteristics, they provide useful in vitro models for study of cellular growth control. The GSH/GSSG redox (Eh) has been strongly implicated to be involved in all three stages of cell development. When induced chemically to differentiate, HT29 cells became more oxidized by 60 mV compared with the proliferative cell population (22). In addition, differentiation induced by butyrate in Caco-2 cells was accompanied by apoptosis (23), which results in a further oxidation of GSH/GSSG redox (6, 17). Present data confirm and extend these results, showing that 1) oxidation of the GSH pool occurs as Caco-2 cells spontaneously progress from proliferation to differentiation, 2) oxidation of the extracellular Cys/CySS pool occurs under the same conditions, 3) increased apoptosis (albeit at low levels) is ...
Study of the human intestines is greatly improved with the addition of an anatomical model. Anatomy Warehouse offers the best in anatomical education, plus free shipping on many orders.
Atıf İçin Kopyala Tunçer S., Banerjee S. Methods in molecular biology (Clifton, N.J.), cilt.1854, ss.55-70, 2019 (Diğer Kurumların Hakemli Dergileri) ...
Maresca M; Mahfoud R; Garmy N; et al. (2003). „The virotoxin model of HIV-1 enteropathy: involvement of GPR15/Bob and galactosylceramide in the cytopathic effects induced by HIV-1 gp120 in the HT-29-D4 intestinal cell line.. J. Biomed. Sci. 10 (1): 156-66. PMID 12566994. doi:10.1159/000068089 ...
Judy D. Whittimore is the author of this article in the Journal of Visualized Experiments: Using Caco-2 Cells to Study Lipid Transport by the Intestine
Balance of forces considered in MSM.(a) Cell monolayer is considered as a thin sheet of cells. Each cell in the monolayer exerts traction, , on the substrate. A
... difficile cells in colorectal cells, and thus prevent Antibiotic Associated Diarrhea. Due to more than a century of safe use, ... bulgaricus strains to Caco-2 Cells" There are also numerous studies that outline the antimicrobial activity of L. bulgaricus ... Adherence to mucus and human epithelial cells and cell lines and the ability to reduce pathogen adhesion to surfaces is ... Resistance to gastric acidity Bile acid resistance Adherence to mucus and/or human epithelial cells and cell lines ...
NF-Y complex serves as a pioneer factor by promoting chromatin accessibility to facilitate other co-localizing cell type- ... "Transcription factor NF-Y regulates differentiation of CaCo-2 cells". Archives of Biochemistry and Biophysics. 407 (1): 39-44. ... Molecular Cell. 55 (5): 708-22. doi:10.1016/j.molcel.2014.07.005. PMC 4157648. PMID 25132174. "Entrez Gene: NFYA nuclear ... Molecular Cell. 55 (5): 708-22. doi:10.1016/j.molcel.2014.07.005. PMC 4157648. PMID 25132174.[verification needed] Oldfield AJ ...
These cells cause B-cells that recognize gliadin to proliferate. The B-cells mature into plasma cells producing anti-gliadin ... May 2007). "Intestinal translocation capabilities of wheat allergens using the Caco-2 cell line". J. Agric. Food Chem. 55 (11 ... p38 MAP kinase activation by CD3 negative cells (Lamina proxima mononuclear cells) and CD83 on dendritic cells Maiuri L, Ciacci ... The resulting structure can be presented by APC (with the same gliadin recognizing DQ isoforms) to T-cells, and B-cells can ...
... helping cell-to-cell spread. After infection, Shigella cells multiply intracellularly and spread to neighboring epithelial ... "Shigella flexneri Enters Human Colonic Caco-2 Epithelial Cells through the Basolateral Pole". Infection and Immunity. 60 (1): ... "N-WASP deficiency reveals distinct pathways for cell surface projections and microbial actin-based motility". Nature Cell ... Hence, Shigella cells may cause dysentery. In rare cases, young children may have seizures. Symptoms can take as long as a week ...
"Identification of Host Cell Factors Associated with Astrovirus Replication in Caco-2 Cells". J. Virol. 89 (20): 10359-70. doi: ... "Alphaherpesvirus axon-to-cell spread involves limited virion transmission". Proceedings of the National Academy of Sciences. ... DNA viruses replicate within the cell nucleus while RNA viruses replicate within the cytoplasm. Exceptions are known to this ... attach to and enter susceptible cells. Basic structural characteristics, such as genome type, virion shape and replication site ...
"Cell atlas - SAAL1 - The Human Protein Atlas". The Human Protein Atlas. Chu PY, Tung SL, Tsai KW, Shen FP, Chan SH (July 2020 ... Immunofluorescent staining has identified SAAL1 localization in the nucleus of Caco-2 cells. However, western blotting of ... doi:10.1016/j.cell.2005.08.029. hdl:11858/00-001M-0000-0010-8592-0. PMID 16169070. S2CID 8235923. "BoxShade Server". ExPASy. " ... doi:10.1016/j.cell.2015.06.043. PMC 4617211. PMID 26186194. "TMIGD3 - Transmembrane domain-containing protein TMIGD3 - Homo ...
The encoded protein is expressed as a precursor protein that is processed into two cell-surface-associated subunits, although ... 2002). "pCLCA1 becomes a cAMP-dependent chloride conductance mediator in Caco-2 cells". Biochem. Biophys. Res. Commun. 298 (4 ... 2003). "A calcium-activated chloride channel blocker inhibits goblet cell metaplasia and mucus overproduction". Novartis Found ... 334 (2): 569-76. doi:10.1016/j.bbrc.2005.06.122. PMID 16023076. CLCA1+protein,+human at the US National Library of Medicine ...
2005). Caco-2 permeability of weakly basic drugs predicted with the Double-Sink PAMPA pKaflux method. Pharm. Sci., 24: 333-349 ... 2004). PAMPA - a drug absorption in vitro model 7. Comparing rat in situ, Caco-2, and PAMPA permeability of fluoroquinolones. ... Drug development Drug discovery Lipid bilayer Caco-2 cell-based permeability Ottaviani, Giorgio; Martel, Sophie; Carrupt, ... including Caco-2 cultures, the gastrointestinal tract, blood-brain barrier and skin. The donor and/or acceptor compartments may ...
"Expression and localization of organic cation/carnitine transporter OCTN2 in Caco-2 cells". American Journal of Physiology. ... "Downregulation of carnitine acyltransferases and organic cation transporter OCTN2 in mononuclear cells in healthy elderly and ... 162 (2): e34-5. doi:10.1016/j.ijcard.2012.05.038. PMID 22658351. Koizumi A, Nozaki J, Ohura T, Kayo T, Wada Y, Nezu J, Ohashi R ... 3.0.CO;2-8. PMID 10612840. Wang Y, Kelly MA, Cowan TM, Longo N (2000). "A missense mutation in the OCTN2 gene associated with ...
1998). "Differential regulation of intestinal and liver fatty acid-binding proteins in human intestinal cell line (Caco-2): ... Cell Res. 244 (2): 441-7. doi:10.1006/excr.1998.4186. PMID 9806794. Darimont C, Gradoux N, de Pover A (1999). "Epidermal growth ... They may also be responsible in the modulation of cell growth and proliferation. Intestinal fatty acid-binding protein 2 gene ... 2: 7. doi:10.1186/1471-2156-2-7. PMC 31346. PMID 11299043. Makowski L, Boord JB, Maeda K, et al. (2001). "Lack of macrophage ...
... which transcytoses bidirectionally in Caco-2 cells". The Journal of Biological Chemistry. 270 (35): 20717-20723. doi:10.1074/ ...
In vitro studies using influenza virus-infected normal human bronchial epithelial cells conducted at the Illinois Institute of ... January 2021). "Enisamium is an inhibitor of the SARS-CoV-2 RNA polymerase and shows improvement of recovery in COVID-19 ... Enisamium iodide effectively inhibited SARS-CoV-2 virus replication in vitro in Caco-2 cells. Enisamium has antiviral activity ... "Enisamium is a small molecule inhibitor of the influenza A virus and SARS-CoV-2 RNA polymerases". bioRxiv. doi:10.1101/2020.04. ...
Shaulian E, Karin M (May 2002). "AP-1 as a regulator of cell life and death". Nature Cell Biology. 4 (5): E131-6. doi:10.1038/ ... Chang CJ, Chao JC (April 2002). "Effect of human milk and epidermal growth factor on growth of human intestinal Caco-2 cells". ... For example, inactivated c-Jun-ER cells show a normal morphology, while c-Jun-ER activated cells have been shown to be ... The process of skin metabolism is initiated by signals that trigger undifferentiated proliferative cells to undergo cell ...
As an antioxidant, tyrosol may protect cells against injury due to oxidation in vitro. Although it is not as potent as other ... protects against oxidized-LDL-induced injury in Caco-2 cells". J. Nutr. 129 (7): 1269-1277. doi:10.1093/jn/129.7.1269. PMID ...
Szymocha R, Akaoka H, Dutuit M, Malcus C, Didier-Bazes M, Belin MF, Giraudon P (July 2000). "Human T-cell lymphotropic virus ... "EAAT1 is involved in transport of L-glutamate during differentiation of the Caco-2 cell line". American Journal of Physiology. ... In the retina, EAAT1 is expressed in Muller cells. EAAT1 is also expressed in a number of other tissues including cardiac ... The coding sequences of 40 new genes (KIAA0041-KIAA0080) deduced by analysis of cDNA clones from human cell line KG-1". DNA ...
... alkaline sphingomyelinase in colon cancer HT-29 cells and a differentiation-related expression of the wild-type enzyme in Caco- ... can inhibit cell proliferation and stimulate cell differentiation and apoptosis. Animal studies showed that supplement of SM or ... alkaline sphingomyelinase in colon cancer HT-29 cells and a differentiation-related expression of the wild-type enzyme in Caco- ... Andersson D, Kotarsky K, Wu J, Agace W, Duan RD (July 2009). "Expression of alkaline sphingomyelinase in yeast cells and anti- ...
Cell Biol. 119 (1): 37-43. doi:10.1007/s00418-002-0479-y. PMID 12548404. S2CID 7822041. Neumann J, Brandsch M (2003). "Delta- ... Sun BW, Zhao XC, Wang GJ, Li N, Li JS (2003). "Hormonal regulation of dipeptide transporter (PepT1) in Caco-2 cells with normal ... "An oligopeptide transporter is expressed at high levels in the pancreatic carcinoma cell lines AsPc-1 and Capan-2". Cancer Res ... "Thyroid hormone regulates the activity and expression of the peptide transporter PEPT1 in Caco-2 cells". Am. J. Physiol. ...
"Effect of self-microemulsifying drug delivery systems containing Labrasol on tight junctions in Caco-2 cells". Eur J Pharm Sci ... "Effect of self-microemulsifying drug delivery systems containing Labrasol on tight junctions in Caco-2 cells". Eur J Pharm Sci ... 371 (1-2): 148-55. doi:10.1016/j.ijpharm.2008.12.009. PMID 19124065. Chen Y; Li G; Wu X; Chen Z; Hang J; Qin B; Chen S; Wang R ... 365 (1-2): 136-42. doi:10.1016/j.ijpharm.2008.08.009. PMID 18782611. Cui J; Yu B; Zhao Y; Zhu W; Li H; Lou H; Zhai G (December ...
The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. ... Frank PG, Lisanti MP (January 2007). "Caveolin-1 and liver regeneration: role in proliferation and lipogenesis". Cell Cycle. 6 ... "Cell-type and tissue-specific expression of caveolin-2. Caveolins 1 and 2 co-localize and form a stable hetero-oligomeric ... Journal of Cell Science. 115 (Pt 23): 4457-67. doi:10.1242/jcs.00130. PMID 12414992. Scherer PE, Lewis RY, Volonte D, Engelman ...
However, AuNPs conjugated with PEG copolymers showed negligible toxicity towards human colon cells (Caco-2). AuNP toxicity also ... cell health, cell stress, how many cells are taken into a cell), and the capping ligands in solution. In vivo assessments can ... AuNSs size 1.4 nm were found to be toxic in human skin cancer cells (SK-Mel-28), human cervical cancer cells (HeLa), mouse ... In certain doses, AuNSs that have positively-charged ligands are toxic in monkey kidney cells (Cos-1), human red blood cells, ...
J. Physiol., Cell Physiol. 292 (1): C332-41. doi:10.1152/ajpcell.00634.2005. PMID 16956961. v t e (Articles with short ... "Regulation of the human taurine transporter by TNF-alpha and an anti-inflammatory function of taurine in human intestinal Caco- ... 1992). "Molecular cloning of the cDNA for an MDCK cell Na(+)- and Cl(−)-dependent taurine transporter that is regulated by ... Han X, Patters AB, Chesney RW (2002). "Transcriptional repression of taurine transporter gene (TauT) by p53 in renal cells". J ...
"Transport and metabolism of flavonoids from Chinese herbal remedy Xiaochaihu-tang across human intestinal Caco-2 cell ...
As of 2021, the current understanding of LNPs formulated with such ionizable cationic lipids is that they enter cells through ... Elucidation of intestinal lymphatic absorption mechanism from solid lipid nanoparticles using Caco-2 cell line as in vitro ... To elucidate the absorption mechanism, from solid lipid nanoparticle, human excised Caco-2 cell monolayer could be alternative ... to bind lipids to nucleic acids in order to transfect the latter into cells. However, by the late 1990s, it was known from in ...
Cell Physiology. 283 (6): C1696-704. doi:10.1152/ajpcell.00461.2001. PMID 12388072. Boyer JC, Campbell CE, Sigurdson WJ, Kuo SM ... "Up-regulation and polarized expression of the sodium-ascorbic acid transporter SVCT1 in post-confluent differentiated CaCo-2 ... "Regulation of the human vitamin C transporters expressed in COS-1 cells by protein kinase C [corrected]". American Journal of ... "Molecular characterization of two novel transporters from human and mouse kidney and from LLC-PK1 cells reveals a novel ...
This anti-inflammatory effect is due to decreased numbers and activity of T helper 17 (Th17) cells and increased numbers of T ... "Prebiotic oligosaccharides reduce proinflammatory cytokines in intestinal Caco-2 cells via activation of PPARγ and ... Bacteria and their products increase the expression of PGLYRP3 in keratinocytes and oral epithelial cells. Mouse PGLYRP3 is ... PGLYRP3 forms disulfide-linked homodimers, but when expressed in the same cells with PGLYRP4, it forms PGLYRP3:PGLYRP4 ...
... alters expression and function of syncytin and its receptor during trophoblast cell fusion of human placental BeWo cells: ... "Functional regulation of Na+-dependent neutral amino acid transporter ASCT2 by S-nitrosothiols and nitric oxide in Caco-2 cells ... "Transcriptional effects of hypoxia on fusiogenic syncytin and its receptor ASCT2 in human cytotrophoblast BeWo cells and in ex ... "The envelope glycoprotein of human endogenous retrovirus type W uses a divergent family of amino acid transporters/cell surface ...
... induce cytotoxicity by altering mechanisms involved in cellular energy supply in the human epithelial cell model Caco-2". ... However, they do have the capacity to disrupt epithelial cells in the human intestine and adversely impact human health. ...
CoA reductase inhibitor mevastatin enhances the growth inhibitory effect of butyrate in the colorectal carcinoma cell line Caco ... High doses inhibit growth and proliferation of melanoma cells. Biosynthesis of mevastatin is primarily accomplished via a type ... lovastatin and mevastatin inhibit proliferation and invasion of melanoma cells". BMC Cancer. 8: 9. doi:10.1186/1471-2407-8-9. ... 2". Carcinogenesis. 22 (7): 1061-7. doi:10.1093/carcin/22.7.1061. PMID 11408350. Brown AG, Smale TC, King TJ, Hasenkamp R, ...
2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130-5. ... "Cathepsin C propeptide interacts with intestinal alkaline phosphatase and heat shock cognate protein 70 in human Caco-2 cells ... "Differentiation-dependent activation of the human intestinal alkaline phosphatase promoter by HNF-4 in intestinal cells". Am. J ... 285 (2): G433-41. doi:10.1152/ajpgi.00466.2002. PMID 12660142. S2CID 30731288. Wada A, Wang AP, Isomoto H, et al. (2005). " ...
2004). "Control of differentiation-induced calbindin-D9k gene expression in Caco-2 cells by cdx-2 and HNF-1α". Am. J. Physiol. ... 185 (2): 663-9. doi:10.1016/0006-291X(92)91676-H. PMID 1610358. Jeung EB, Krisinger J, Dann JL, Leung PC (Sep 1992). "Molecular ... 307 (2): 224-8. doi:10.1016/0014-5793(92)80772-9. PMID 1379540. S2CID 27787157. "Entrez Gene: S100G S100 calcium binding ...
Unit cell[edit]. All sources give unit cell parameters whose values rounded down are a = 8.1 Å, b = 5.2 Å, c = 6.5 Å and β = ... Calcite CaCO3 and dolomite CaMg(CO3)2 are carbonates where calcium Ca and magnesium Mg replace the barium in the formula for ... Unit cell. a = 8.1 Å, b = 5.2 Å, c = 6.5 Å, β = 106°; Z = 2. ... 106°, with two formula units per unit cell (Z = 2). The primary source[5] has a = 8.092 Å, b = 5.2344 Å, c = 6.544 Å, β = ...
... influence of cell and culture-related factors on Caco-2 cell functional characteristics". Cell Biology and Toxicology. 21 (1): ... These include the descending limb cells, thin ascending limb cells, thick ascending limb cells, cortical collecting duct cells ... 3D cell constructs mimic the in vivo environment[44] and the interactions (e.g., cell to cell) occurring in the human body[45] ... was developed and included lung cells, drug-metabolizing liver and fat cells. The cells were linked in a 2D fluidic network ...
A molecule called nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) is known to play a critical role in ... CaCO 3 (s) + 2 NaOCl (aq) Na 2CO 3 (aq) + CaCl 2 (aq) → CaCO 3 (s) + 2 NaCl (aq) Na 2CO 3 (aq) + Ca(OH) 2 (s) → CaCO 3 (s) + 2 ... with increased cell proliferation. The effect diminished after the baths were stopped, indicating that regular exposure was ... At pH below 2, the majority of the chlorine in the solution is in the form of dissolved elemental Cl 2. At pH greater than 7.4 ...
Matrix Chemistry Controlling Stem Cell Behavior", Biology and Engineering of Stem Cell Niches, Boston: Academic Press, pp. 195- ... "Analysis of glucosamine and chondroitin sulfate content in marketed products and the Caco-2 permeability of chondroitin sulfate ... Over 2 years, none of the treatments (not even the positive control celecoxib) achieved a clinically important difference in ... 211 (2): 605-11. doi:10.1016/S0021-9258(18)71150-2. PMID 13221568. Free PDF online Archived 2008-09-22 at the Wayback Machine ...
"Cholesterol and sphingomyelin syntheses are regulated independently in cultured human intestinal cells, CaCo-2: role of ... Cell membranes require high levels of cholesterol - typically an average of 20% cholesterol in the whole membrane, increasing ... The bilayer formed by membrane lipids serves as a containment unit of a living cell. Membrane lipids also form a matrix in ... Functional roles of lipids are in fact many: They serve as regulatory agents in cell growth and adhesion. They participate in ...
Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals ... in human epithelial caco-2 cells". The Journal of Biological Chemistry. 276 (12): 9291-6. doi:10.1074/jbc.M006991200. PMID ... a possible role for JAMs in endothelial cell polarity". Journal of Cell Science. 116 (Pt 19): 3879-91. doi:10.1242/jcs.00704. ... Ebnet K, Suzuki A, Horikoshi Y, Hirose T, Meyer Zu Brickwedde MK, Ohno S, Vestweber D (Jul 2001). "The cell polarity protein ...
Ca, Co, Cu, Fe, K, Mo, Mn, Mg, Ni, and Zn. Ammonium chloride, ammonium sulfate, or ammonium phosphate may be used as sources of ... ruptured cells) Lecithin, monoglycerides, diglycerides, and DATEM are considered emulsifiers. They disperse fat more evenly ... 57 (2): 85-87. Archived from the original (PDF) on 2013-05-04. Retrieved 2017-01-23. Elkassabany, M.; Hoseney, R.C. (1980). " ... 57 (2): 88-91. Popper, Lutz. "18.3 Oxidation and Flour Maturation" (PDF). "Sodium Bisulfite". BAKER-pedia. Brown, Amy L. (2008 ...
These membranes are flat sheets that form a continuous barrier around all cells. The cell membranes of almost all organisms and ... Avdeef, A.; Artursson, P.; Neuhoff, S.; Lazorova, L.; Gråsjö, J.; Tavelin, S. (2005). "Caco-2 permeability of weakly basic ... This procedure is now used extensively, for example by fusing B-cells with myeloma cells. The resulting "hybridoma" from this ... Koch AL (1984). "Primeval cells: possible energy-generating and cell-division mechanisms". J. Mol. Evol. 21 (3): 270-7. Bibcode ...
... enteroendocrine cells, goblet cells, transit amplifying cells, paneth cells and intestinal stem cells. Over the years, the ... Hidalgo IJ, Raub TJ, Borchardt RT (March 1989). "Characterization of the human colon carcinoma cell line (Caco-2) as a model ... Pinto M (1983). "Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture". ... a non cell-based permeability assay Lea, Tor (2015). "Caco-2 Cell Line". The Impact of Food Bioactives on Health: 103-111. doi: ...
... and human colon cancer cells (Caco-2) in comparison with quercetin itself. Kojic acid has been shown to protect Chinese hamster ... ovary cells against ionizing radiation-induced damage. When exposed to a lethal dose of 3 Gy gamma radiation, dogs pretreated ... 102 (2): 211. doi:10.3987/REV-20-936. (Webarchive template wayback links, ECHA InfoCard ID from Wikidata, Pages using ... cocrystals of kojic acid with quercetin were found to have two times better cytotoxic activity to human cervical cancer cells ( ...
The equation for this is: T = A + B ⋅ ( ( δ O 18 ) CaCO 3 ⏞ calcite − ( δ O 18 ) H 2 O ⏞ water ) {\displaystyle T=A+B\cdot \ ... and ions leached from the target cell and sputtered from the Havar foil. Willi Dansgaard - a paleoclimatologist Isotopes of ... Labeling by 18 O 2 gives the unidirectional flux of O 2 uptake, while there is a net photosynthetic 16 O 2 evolution. It was ... Kendall, C.; Caldwell, E.A. (1998). "Chapter 2: Fundamentals in Isotope Geochemistry". Isotope Tracers in Catchment Hydrology. ...
... cell line LoVo cells, compared with other three colorectal cell lines SW480, HT29 and Caco-2. Overexpression of miR-26b ... "Human embryonic stem cells and metastatic colorectal cancer cells shared the common endogenous human microRNA-26b". J Cell Mol ... Overexpression of miR-26b in DFOM-treated CNE cells inhibits cell proliferation through targeting COX-2. Breast cancer miR-26b ... mir-26a is strongly downregulated in melanoma cells compared with primary melanocytes. Treatment of melanoma cell lines with a ...
Bioavailability Blood plasma Caco-2 Cheminformatics Combinatorial chemistry Drug metabolism Lipinski's rule of five Parallel ... before being taken up by the target cells. Factors such as poor compound solubility, gastric emptying time, intestinal transit ... 7 (2): 165-182. doi:10.2174/138920006775541552. PMID 16472106. Tetko IV, Bruneau P, Mewes HW, Rohrer DC, Poda GI (August 2006 ...
Hidalgo IJ, Raub TJ, Borchardt RT (March 1989). "Characterization of the human colon carcinoma cell line (Caco-2) as a model ... Not all cancer cells have cell entry receptors for the virus and not all cancer cells express virus processing serine proteases ... involves the fusion of infected cells with adjacent target cells and remains an important mechanism of direct cell-to-cell ... "Directed cell fusion" below). The virus can fuse up to 50-100 cells adjacent to one primary infected cell. This multi-nuclear ...
... cos cells MeSH A11.251.210.190 - cell line, tumor MeSH A11. - caco-2 cells MeSH A11. - hct116 ... cho cells MeSH A11.251.210.505 - l cells (cell line) MeSH A11.251.210.520 - llc-pk1 cells MeSH A11.251.210.700 - 3t3 cells MeSH ... l cells MeSH A11.329.228.900 - 3t3 cells MeSH A11.329.228.900.080 - balb 3t3 cells MeSH A11.329.228.900.550 - nih 3t3 cells ... hela cells MeSH A11. - kb cells MeSH A11. - hl-60 cells MeSH A11. - ht29 cells ...
IBC is characterised by the presence of cancer cells in the subdermal lymphatics on skin biopsy. Consequently, IBC is always ... CACO.0000024222.61114.18. JSTOR 3554049. PMID 15090727. S2CID 25585518. Gordon, L (2001). "Inflammatory breast cancer". ... and may contribute to tumour cell motility E-cadherin is overexpressed; paradoxically, it is associated with especially ... 2 April 2009 Kusama, M; Koyanagi, Y; Sekine, M; Serizawa, H; Ebihara, Y; Hirota, T; Nakamura, Y; Matsunaga, T (1994). "A case ...
Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) also known as CD66e (Cluster of Differentiation 66e), is a ... by members of the Afa/Dr diffusely adhering family of Escherichia coli that infect the human polarized intestinal Caco-2/TC7 ... Also, it plays a significant role in the inhibition of differentiation and apoptosis in colon cells. There are evidences that ... August 1987). "Carcinoembryonic antigen family: expression in a mouse L-cell transfectant and characterization of a partial ...
... of the test allow the transfer of material from the pseudopodia to the internal cell and back. The foraminiferal cell is ... These shells are commonly made of calcium carbonate (CaCO 3) or agglutinated sediment particles. Over 50,000 species are ... The B form larvae are produced inside of the cyst; any nuclei that are not bound into cells are consumed as food for the ... When two gametes combine, they create a diploid, multi-nucleated cell known as the agamont, or B form. In contrast to the ...
For example, hopanoids are just parts of the bacterial cell wall present in oil as a contaminant. Nickel (Ni), vanadium (V), ... CaCO_{3}\rightarrow CH_{4}+CaO+2H_{2}O} } Note that CaO (lime) is not a mineral species found within natural rocks. Whilst this ... Experiments in diamond anvil high pressure cells have resulted in partial conversion of methane and inorganic carbonates into ... 2009). "In Situ Diamond-Anvil Cell Observations of Methanogenesis at High Pressures and Temperatures". Energy Fuels. 23 (11): ...
"Tumour suppressor microRNA-584 directly targets oncogene Rock-1 and decreases invasion ability in human clear cell renal cell ... Liao Y, Lönnerdal B (August 2010). "miR-584 mediates post-transcriptional expression of lactoferrin receptor in Caco-2 cells ... Biochemistry and Cell Biology. 90 (3): 476-84. doi:10.1139/o11-075. PMID 22332905. Ueno K, Hirata H, Shahryari V, Chen Y, Zaman ... "Differential miRNA expression and their target genes between NGX6-positive and negative colon cancer cells". Molecular and ...
"Genistein at a concentration present in soy infant formula inhibits Caco-2BBe cell proliferation by causing G2/M cell cycle ... At low doses, genistein acted as a weak estrogen and stimulated cell growth; at high doses, it inhibited proliferation and ... Some studies have found that some concentrations of isoflavones may have effects on intestinal cells. ... altered cell cycle dynamics. This biphasic response correlates with how genistein is thought to exert its effects. Some reviews ...
A variety of models have been used including yeast, zebrafish, worms, immortalized cell lines, primary cell lines, patient- ... PAMPA is attractive as an early screen due to the low consumption of drug and the low cost compared to tests such as Caco-2, ... or cell proliferation as examples in a more holistic cell model or organism. Smaller screening sets are often used for these ... which pull the chromosomes apart in dividing cells (such as cancer cells). Other examples are: 1. Camptotheca (Camptothecin · ...
... is the major component in forming cell membranes, enables smooth cell division and removes harmful substances by binding with ... However, the majority exists in the form of calcium carbonate (CaCO 3), which cannot be directly absorbed by plants. Egg, clam ... Deficiency hinders cell division and reproduction. Symptoms first appear on the petiole and veins of older leaves. New leaves ... Phosphoric acid makes up part of the cell nucleus and reproductive system. Phosphoric acid is involved in photo phosphorylation ...
... in epithelial cells instead of sites at basolateral areas of cell-cell contact containing tyrosine-phosphorylated p120 and ... PLEKHA7 was identified by mass spectrometry in lysates of human intestinal carcinoma (Caco-2) cells in a GST-pull down using N- ... "Anchorage of microtubule minus ends to adherens junctions regulates epithelial cell-cell contacts". Cell. 135 (5): 948-59. doi: ... However, in Eph4 cell line, PLEKHA7 is recruited to E-cadherin based AJ by Afadin, independently of p120. PLEKHA7 knockdown ...
7,8-DHF was poorly transported across Caco-2 cell monolayers by mainly passive diffusion via a transcellular pathway and not a ... We discussed the transport mechanisms of 7,8-DHF using the Caco-2 cell model to determine the bi-directional permeability with ... Transepithelial transport mechanisms of 7,8-dihydroxyflavone, a small molecular TrkB receptor agonist, in human intestinal Caco ... Transepithelial transport mechanisms of 7,8-dihydroxyflavone, a small molecular TrkB receptor agonist, in human intestinal Caco ...
Effect of Erica australis extract on Caco-2 cells, fibroblasts and selected pathogenic bacteria responsible for wound infection ...
Effect of Erica australis extract on Caco-2 cells, fibroblasts and selected pathogenic bacteria responsible for wound infection ...
Regarding to Caco-2 cells, the coloured extract exhibited the most cytotoxic effects, while the total extract was the most ... protection and effects on Caco-2 cells. A total of 22 phenolic compounds were identified by LC-DAD. Hydroxycinnamic acids were ... efficient in protecting these cells against oxidative damage induced by t-BHP. ... Regarding to Caco-2 cells, the coloured extract exhibited the most cytotoxic effects, while the total extract was the most ...
These findings indicate that C. asiatica extract inhibited cell proliferation of Caco-2 cells through modification of the cell ... The mechanism of cancer cell growth inhibition was shown to occur via cell cycle arrest. The extract induced S and G2-M arrest ... CENTELLA ASIATICA EXTRACT INDUCES CELL CYCLE ARREST IN CACO-2 HUMAN COLON CANCER CELLS. ... C. asiatica extract reduced the proliferation rate of Caco-2 cells significantly in a concentration- and time-dependent manner ...
Two-and three-dimensional QSAR of carrier-mediated transport of beta-lactam antibiotics in Caco-2 cells. Author Suchada ... Two-and three-dimensional QSAR of carrier-mediated transport of beta-lactam antibiotics in Caco-2 cells ...
40 bacterial cells/Caco-2 cells. Low cytotoxicity percentages were observed for the candidate LAB strains with values ranging ... CD4 cell count , 500 cells/muL (PR 2.59; 95% CI 1.46-4.61), viral load , 400 copies/mL (PR 3.98; 95% CI 1.25-12.6), highly ... We have previously described a vaccine candidate composed of single-cycle LASV replicon particles (VRPs) and a stable cell line ... Endowment of iron-rich red blood cells obtained through DCC may offer a longitudinal advantage for early white matter ...
Caco2 cells were treated with increasing concentrations of PEA (0.001, 0.01 and 0.1 μM) in the presence of peroxisome ... Caco2 cells were treated with increasing concentrations of PEA (0.001, 0.01 and 0.1 μM) in the presence of peroxisome ... Cell proliferation was evaluated by performing a MTT assay. Vascular endothelial growth factor (VEGF) release was estimated by ... Cell proliferation was evaluated by performing a MTT assay. Vascular endothelial growth factor (VEGF) release was estimated by ...
The in vitro absorption study indicated that theasinensins can be transported across Caco-2 cell monolayers, while their ... The in vitro absorption study indicated that theasinensins can be transported across Caco-2 cell monolayers, while their ... The in vitro absorption study indicated that theasinensins can be transported across Caco-2 cell monolayers, while their ... The in vitro absorption study indicated that theasinensins can be transported across Caco-2 cell monolayers, while their ...
Serotonin Receptors, Novel Targets of Sulforaphane Identified by Proteomic Analysis in Caco-2 Cells ... Novel Targets of Sulforaphane Identified by Proteomic Analysis in Caco-2 Cells. Cancer Research, 68 (13). pp. 5487-5491. ...
in Caco-2 Cell Model. Bioorg.Med Chem Lett 2-15-2007;17:1107-1111. View abstract. ... Overholser, C. D., Meiller, T. F., DePaola, L. G., Minah, G. E., and Niehaus, C. Comparative effects of 2 chemotherapeutic ... Eucalyptus microcorys leaf extract derived HPLC-fraction reduces the viability of MIA PaCa-2 cells by inducing apoptosis and ... arresting cell cycle. Biomed Pharmacother. 2018;105:449-460. View abstract.. *Soonwera M, Wongnet O, Sittichok S. Ovicidal ...
Sidebar listing: list and traverse vocabulary contents by a ...
We investigated basolateral transport of GABA in mature Caco-2 cell monolayers using isotope studies. Here we report that, at ... Carrier-mediated ¿-aminobutyric acid transport across the basolateral membrane of human intestinal Caco-2 cell monolayers. ... across the basolateral membrane of intestinal cells. The proton-coupled amino acid transporter, hPAT1, mediates the influx of ... The basolateral uptake consisted of a high-affinity system with a K(m) of 290µM and V(max) of 75pmolcm(-2)min(-1) and a low ...
These active compounds were also effective in colon carcinoma Caco-2 cells, and some of them increased the expression of p62 ... Identification of Dietary Phytochemicals Capable of Enhancing the Autophagy Flux in HeLa and Caco-2 Human Cell Lines ... and zerumbone enhanced the autophagy flux in HeLa cells. Meanwhile, analysis of the structure-activity relationships indicated ... While isorhamnetin and chrysoeriol enhanced autophagy in an mTOR signaling-dependent manner, the actions of 2,2′,4′- ...
... as well as in a more aggressive transformed phenotype compared with empty vector-transfected control cells. In contrast, cells ... Transfection of CaCo-2 cells with PKC-α in the antisense orientation resulted in enhanced proliferation and decreased ... as well as in a more aggressive transformed phenotype compared with empty vector-transfected control cells. In contrast, cells ... as well as in a more aggressive transformed phenotype compared with empty vector-transfected control cells. In contrast, cells ...
The analysis of Caco-2 subclones stably transfected with an H-chain promoter-chloramphenicol acetyltransferase (CAT) construct ... The analysis of Caco-2 subclones stably transfected with an H-chain promoter-chloramphenicol acetyltransferase (CAT) construct ... The analysis of Caco-2 subclones stably transfected with an H-chain promoter-chloramphenicol acetyltransferase (CAT) construct ... The analysis of Caco-2 subclones stably transfected with an H-chain promoter-chloramphenicol acetyltransferase (CAT) construct ...
Aluminium in Alzheimers disease: are we still at a crossroad?. Cell Mol Life Sci. 2005 Jan. 62(2):143-58. [QxMD MEDLINE Link] ... Fatty acids increase paracellular absorption of aluminium across Caco-2 cell monolayers. Chem Biol Interact. 2009 Oct 7. 181(2 ... Aluminum-induced defective mitochondrial metabolism perturbs cytoskeletal dynamics in human astrocytoma cells. J Neurosci Res. ... In vivo genotoxicity assessment of aluminium oxide nanomaterials in rat peripheral blood cells using the comet assay and ...
The differentiation status of cells and the impact of CuO NMs and CuSO4 on the integrity of the differentiated Caco-2 cell ... A reduction in cell viability in differentiated cells was not responsible for the lower level of cytokine production observed. ... CuO NMs and CuSO4 caused a concentration dependent decrease in cell viability in undifferentiated cells. CuO NMs and CuSO4 ... The impact of CuO NMs and CuSO4 on the viability of differentiated cells was performed via assessment of cell number (DAPI ...
Milk modulates campylobacter invastion into CACO-2 intestinal epithelial cells. Milk modulates campylobacter invastion into ...
The toxicity of cleaved PT-gliadin products was assessed in Caco-2 epithelial cells, celiac patient-derived T cells, and in ... Pretreated gliadin did not stimulate T cell proliferation in vitro or enhance the production of autoantibodies to culture ... induce cytoskeletal rearrangement or changes in ZO-1 expression in Caco-2 cells. ...
... difficile cells in colorectal cells, and thus prevent Antibiotic Associated Diarrhea. Due to more than a century of safe use, ... bulgaricus strains to Caco-2 Cells" There are also numerous studies that outline the antimicrobial activity of L. bulgaricus ... Adherence to mucus and human epithelial cells and cell lines and the ability to reduce pathogen adhesion to surfaces is ... Resistance to gastric acidity Bile acid resistance Adherence to mucus and/or human epithelial cells and cell lines ...
... in Caco2 cell line. Inhibition percent of each sample (%) was compared with a control group (Caco-2 cell with Rh 123). Results ... exhibited the highest Rh123 percent in Caco-2 cells that could be associated with inhibitory effect on P-gp efflux activity. ... in Caco2 cell line. Inhibition percent of each sample (%) was compared with a control group (Caco-2 cell with Rh 123). Results: ... Evaluation of some medicinal plants effect on Rhodamine 123 accumulation and efflux in Caco-2 cell line by flowcytometry. ...
ERK1/2, and JNK), and TLR4/NF-,i,κ,/i,B signaling pathways but promoted the activation of the PI3K/AKT pathway. A ... The human intestinal cell line Caco-2 is commonly used as an effective in vitro model to study the passive diffusion of drugs ... and Caco-2 cell as candidate active molecules (Table 1). There were 9, 10, 5, 11, and 31 active compounds in HQ, WWZ, FL, SHHZ ... and Caco-2 cell . Among them, quercetin (MOL02), formononetin (MOL13), kaempferol (MOL15), taxifolin (MOL21), cryptotanshinone ...
Effects of lymphocytes, macrophages and microparticles on tight junction permeability in Caco-2 cells ... Effects of lymphocytes, macrophages and microparticles on tight junction permeability in Caco-2 cells ...
Differential induction of apoptosis in human colonic carcinoma cells (Caco-2) by Atopobium, and commensal, probiotic and ... gut bacteria within the colon cancer cell line, Caco-2. The results show a major apoptotic effect for the pathogens, mild ... GASTRIC EPITHELIAL-CELLS, ESCHERICHIA-COLI, CANCER-CELLS, UP-REGULATION, CYCLE ARREST, BIFIDOBACTERIUM-LACTIS, RECEPTOR ... Altonsy, M. O., Andrews, S. C. and Tuohy, K. M. (2010) Differential induction of apoptosis in human colonic carcinoma cells ( ...
Mediation of Highly Concentrative Uptake of Pregabalin by L-Type Amino Acid Transport in Chinese Hamster Ovary and Caco-2 Cells ... Mediation of Highly Concentrative Uptake of Pregabalin by L-Type Amino Acid Transport in Chinese Hamster Ovary and Caco-2 Cells ... Mediation of Highly Concentrative Uptake of Pregabalin by L-Type Amino Acid Transport in Chinese Hamster Ovary and Caco-2 Cells ... Mediation of Highly Concentrative Uptake of Pregabalin by L-Type Amino Acid Transport in Chinese Hamster Ovary and Caco-2 Cells ...
Panax notoginseng saponin attenuates gastric mucosal epithelial cell injury induced by dual antiplatelet drugs.Jan 14, 2021. ... Natural polyphenols prevent indomethacin-induced and diclofenac-induced Caco-2 cell death.Jan 09, 2020. ...
Expression data from Caco-2 cells co-cultivated with B. animalis subsp. lactis BB-12. Add to Dataset. ... In this study we examined immune response of Caco-2 enterocytes after incubation with common probiotic Bifidobacterium animalis ...
  • Inhibition of Akt phosphorylation and a downstream decrease of phospho‐mTOR and of p‐p70S6K were observed as compared with untreated cells. (
  • The mechanism of cancer cell growth inhibition was shown to occur via cell cycle arrest. (
  • Mechanisms of toxicity include inhibition of enzyme activity and protein synthesis, alterations in nucleic acid function, and changes in cell membrane permeability. (
  • Inhibition percent of each sample (%) was compared with a control group (Caco-2 cell with Rh 123). (
  • The increased tissue exposure to DPC in rats could largely be attributed to inhibition of CYP3A1/2 by RTV. (
  • Transported metabolites were investigated by UPLC MS/MSE, and the effects on NO production, ROS inhibition and secretion of vascular endothelial growth factor (VEGF), interleukin-8 (IL-8) and intercellular adhesion molecule-1 (ICAM-1) were evaluated in TNF-alpha-activated and nonactivated endothelial cells. (
  • Knocking out GSDME switched lobaplatin-induced cell death from pyroptosis to apoptosis but did not affect lobaplatin-mediated inhibition of growth and tumour formation of HT-29 and HCT116 cells in vivo and in vitro. (
  • Mycophenolate Mofetil induces caspase-dependent apoptosis and cell cycle inhibition in multiple myeloma cells. (
  • We explored the effects of DADS and one of its metabolites, allyl mercaptan (AM), on histone deacetylase (HDAC) activity: using nuclear extracts of Caco-2 cells, 200 microM DADS decreased HDAC activity by 29% and AM at the same concentration was more efficient (92% inhibition). (
  • Our results suggest that in Caco-2 and HT-29 cells, DADS could inhibit cell proliferation through the inhibition of HDAC activity, histone hyperacetylation and increase in p21(waf1/cip1) expression. (
  • We previously demonstrated that the n3-PUFA EPA prevents TNF-alpha inhibition of sugar uptake in Caco-2 cells. (
  • The activity of these peptides was determined by inhibition of Caco-2 single cells that bind to a Caco-2 monolayer and inhibition of junction resealing of MDCK II cell monolayers. (
  • Stat3 inhibition by overexpression of dominant-negative Stat3-D mutant in MDCK cells or by small interfering RNA-mediated knockdown in Caco-2 cells resulted in inhibition of the cell density-induced NHE3 expression, Sp1/Sp3 binding, and NHE3 promoter activity and in decreased dome formation. (
  • Inhibition of Caco-2 and HeLa proliferation by Terminalia carpentariae C. T. White and Terminalia grandiflora Benth. (
  • Home / Pharmacogn J, Vol 9, Issue 4, Jul-Aug, 2017 / Inhibition of Caco-2 and HeLa proliferation by Terminalia carpentariae C. T. White and Terminalia grandiflora Benth. (
  • The extract induced S and G2-M arrest in Caco-2 cells accompanied apoptosis induction. (
  • The induction of apoptosis in mammalian cells by bacteria is well reported. (
  • This process may assist infection by pathogens whereas for non-pathogens apoptosis induction within carcinoma cells protects against colon cancer. (
  • Here, apoptosis induction by a major new gut bacterium, Atopobium minutum, was compared with induction by commensal (Escherichia coli K-12 strains), probiotic (Lactobacillus rhamnosus, Bifidobacterium latis) and pathogenic (E. coli: EPEC and VTEC) gut bacteria within the colon cancer cell line, Caco-2. (
  • Although bacterial infection increased Caco-2 membrane FAS levels, caspase-8 was not activated indicating that apoptosis is FAS independent. (
  • 2003. Promotion versus suppression of rat colon carcinogenesis by chlorophyllin and chlorophyll: modulation of apoptosis, cell proliferation, and beta-catenin/Tcf signaling. . (
  • ASP-A decreased the growth of various human cancer cells in vitro and induced apoptosis in BEL-7402 cells via a caspase-dependent pathway. (
  • CPI-613 induces apoptosis in pancreatic cancer cells. (
  • MAIN METHODS: HEK293 cells transfected with plasmids expressing control vector, ORF8, ORF10, or M protein were assayed for cell number and markers of apoptosis at 24 h, and interferon and interferon-stimulated gene expression at 14 h, with or without CBD. (
  • KEY FINDINGS: Reduced cell number and increased early and late apoptosis were found when expression of viral genes was combined with 1-2 µM CBD treatment, but not in control-transfected cells treated with CBD, or in cells expressing viral genes but treated only with vehicle. (
  • CBD also augmented expression of these genes in control cells not expressing viral genes, but without enhancing apoptosis. (
  • Zinc depletion of cells incubated with TPEN, a specific zinc chelator, caused an increase of tight junction permeability in OTA treated cells, accompanied by increased apoptosis. (
  • The aim of this study was to investigate the effect of selected legumes in inducing cytotoxicity, apoptosis and/or necrosis in human epithelial colorectal adenocarcinoma cells. (
  • The ability of legume extracts in inducing cell apoptosis was analyzed at selected concentrations of 1.5, 3, 6 and 12 mg mL 1 by quantifying DNA fragmentation. (
  • Specific morphological changes related to apoptosis such as: surface blebbing, formation of apoptotic bodies, blisters and echinoid spikes and cell membrane destabilization were observed in cells incubated with extracts. (
  • It was revealed that this compound increased the Bax / Bcl2 ratio in the MCF-7 cells and induced apoptosis (27%) and necrosis (18%) in the cells. (
  • decreased mobile apoptosis, necrosis, and inflammatory cell infiltration in comparison to sham VNS. (
  • The results suggested that Luteolin isolated from Avicennia marina could probably induce apoptosis on breast cancer cell line by the regulation of p53 and Bcl-2 pathways. (
  • Drug-induced apoptosis and p53, bcl-2 and bax expression in breast cancer tissues in vivo and in fibroblast cells in vitro. (
  • We discussed the transport mechanisms of 7,8-DHF using the Caco-2 cell model to determine the bi-directional permeability with different environmental factors (time, concentration, pH, metabolic inhibitors etc. ). (
  • The correlation between the in vitro apparent permeability (P¬app) across Caco-2 monolayers and the in vivo fraction absorbed (fa) is well established.Transwell diagram Cell culture Drug development Pre-clinical development PAMPA - a non cell-based permeability assay Lea, Tor (2015). (
  • Proteases from germinating wheat degraded gliadin into small peptide fragments, which, unlike unprocessed PT-gliadin, did not increase epithelial permeability, induce cytoskeletal rearrangement or changes in ZO-1 expression in Caco-2 cells. (
  • Generally, small molecular inhibitors with oral bioavailability are more suitable for intracellular targets, i.e., viral proteases, by the necessity of cell permeability. (
  • Paracellular permeability and protein and gene expression analysis using DNA microarrays were performed on filter-grown Caco-2 monolayers. (
  • Our previous study demonstrated the anti-inflammatory potency of γ-EV against vascular inflammation at a concentration of 1mM, and that it can transport with the apparent permeability coefficient (P app ) of 1.56 x 10 -6 ± 0.7 x 10 -6 cm/s across the intestinal Caco-2 cells. (
  • The purpose of the current study was to explore whether the permeability of the peptide could be enhanced and to elucidate the mechanism of transport of γ-EV across Caco-2 cells. (
  • The results obtained for the in vitro permeability studies (Caco-2 monolayer studies) showed low permeability for baclofen raw material in the apical to basolateral direction and in the basolateral to apical direction. (
  • Gokulan K, Williams K, Orr S, Khare S. Human Intestinal Tissue Explant Exposure to Silver Nanoparticles Reveals Sex Dependent Alterations in Inflammatory Responses and Epithelial Cell Permeability. (
  • The basolateral uptake consisted of a high-affinity system with a K(m) of 290µM and V(max) of 75pmolcm(-2)min(-1) and a low affinity system with a K(m) of approximately 64mM and V(max) of 1.6nmolcm(-2)min(-1). (
  • Polarized caco-2 cells express tight junctions, microvilli, and a number of enzymes and transporters that are characteristic of such enterocytes: peptidases, esterases, P-glycoprotein, uptake transporters for amino acids, bile acids, carboxylic acids, etc. (
  • Finally, an uptake and transport assay of ASP-A was performed in monolayers of Caco-2 cells, and ASP-A was shown to be absorbed through the active transport pathway. (
  • Current knowledge about the uptake transporters expressed at the BBB and brain parenchymal cells has been used for delivery of CNS drugs to the brain via targeting transporters. (
  • An in vitro experiment was conducted to evaluate the uptake and solubility of zinc provided as Kemtrace Zn MP and zinc sulphate by caco-2 cells after ruminal and intestinal digestion. (
  • For the uptake experiment, caco-2 cells were grown in plastic tissue culture wells using standard tissue culture method. (
  • Drug loading, in vitro release profile, cell-uptake study and in vitro cytotoxicity of [email protected] were tested. (
  • We therefore evaluated the effects of O. formigenes culture conditioned medium (CM) on apical 14 C-oxalate uptake by human intestinal Caco-2-BBE cells. (
  • M. G. Ferruzzi, M. L. Failla and S. J. Schwartz, "Assessment of Degradation and Intestinal Cell Uptake of Carotenoids and Chlorophyll Derivatives from Spinach Puree Using an in Vitro Digestion and Caco-2 Human Cell Model," Journal of Agricultural and Food Chemistry, Vol. 49, No. 4, 2001, pp. 2082-2089. (
  • Citotoxicity and cell uptake will be evaluated in Caco-2 cell monoculture and the biopharmaceutical performance will be evaluated for selected prototypes after oral administration in a rat model.the biopharmaceutical performance will be evaluated after oral administration in a rat model. (
  • 2005). Modulation of MPP+ uptake by tea and some of its components in Caco-2 cells . (
  • Often, dedicated bioassays (e.g. cell culture models for binding and uptake, kinetic profiling or pharmacological activity) need to be available right next to the bioreactor to obtain the necessary information for efficient optimization. (
  • A549, Caco-2, HeLa and HepG2 whole cell lysate ( ab7900 ). (
  • Also, the EO showed considerable cytotoxicity against MCF-7, Caco-2, SH-SY5Y, and HepG2 after 24 and 48 h treatment with IC 50 values between 0.0.47 to 0.81 mg/mL. (
  • in hepatocarcinoma HepG2 cell line. (
  • The aim of this study was to investigate the effect of different concentrations of antioxidants on ABCA1 mRNA expression in TNF-α stimulated hepatocarcinoma (HepG2) cell line. (
  • This study successfully profiled that all the three antioxidants were able to alleviate the downregulatory effect of TNF-α on ABCA1 transcript level in HepG2 cells. (
  • Transient transfection of various human apoAI gene plasmid constructs into human hepatoma (HepG2) and colon carcinoma (Caco-2) cells showed that apoAI gene transcription is under the control of two separate and distinct cell-specific promoters. (
  • The region between nucleotides -192 and -41 is essential for expression in HepG2 cells, whereas the region from -595 to -192 is essential for expression in Caco-2 cells. (
  • Overexpression of the orphan receptor hepatocyte nuclear factor 4 (HNF-4), which binds to the NHRRE, dramatically stimulates apoAI gene expression in Caco-2 cells but not in HepG2 cells. (
  • Generation of HepG2 Cells with High Expression of Multiple Drug-Metabolizing Enzymes for Drug Discovery Research Using a PITCh System. (
  • Protein kinase C regulates the citrate transport via Na + -coupled citrate transporter NaCT in HepG2 cells. (
  • 7,8-DHF was poorly transported across Caco-2 cell monolayers by mainly passive diffusion via a transcellular pathway and not a paracellular pathway. (
  • In conclusion, the dimers of condensed catechins, theasinensins B and A, can be absorbed intact into rat blood and transported across Caco-2 cell monolayers probably through a TJ paracellular pathway. (
  • Cell proliferation was evaluated by performing a MTT assay. (
  • PEA caused a significant and concentration‐dependent decrease of Caco‐2 cell proliferation at 48 h. (
  • PEA is able to decrease cell proliferation and angiogenesis. (
  • C. asiatica extract reduced the proliferation rate of Caco-2 cells significantly in a concentration- and time-dependent manner. (
  • These findings indicate that C. asiatica extract inhibited cell proliferation of Caco-2 cells through modification of the cell cycle events and this cell cycle arrest is associated, at least in part, with increased accumulation of cyclin B1 protein. (
  • Transfection of CaCo-2 cells with PKC-α in the antisense orientation resulted in enhanced proliferation and decreased differentiation, as well as in a more aggressive transformed phenotype compared with empty vector-transfected control cells. (
  • In contrast, cells transfected with PKC-α cDNA in the sense orientation demonstrated decreased proliferation, enhanced differentiation, and an attenuated tumor phenotype compared with these control cells. (
  • These data show that alterations in the expression of PKC-α induce changes in the proliferation, differentiation, and tumorigenicity of CaCo-2 cells. (
  • Pretreated gliadin did not stimulate T cell proliferation in vitro or enhance the production of autoantibodies to culture supernatants and the activation of CD25+ lymphocytes in the organ culture to the same extent as unprocessed PT-gliadin. (
  • Furthermore, cell proliferation was measured in an epithelial wound healing assay and the expression of the inflammatory markers cyclooxygenase-2 (COX-2) and transforming growth Factor-β (TGF-β) was evaluated. (
  • Cell proliferation under HS conditions was improved by ALA supplementation as demonstrated in an epithelial wound healing assay and ALA was able to affect the HS-induced inflammatory response by decreasing the COX-2 and TGF-β mRNA expression. (
  • Conclusions: ALA supplementation could prevent the disruption of intestinal epithelial integrity by enhancing epithelial cell proliferation, and reducing the inflammatory response under HS conditions in an in vitro Caco-2 cell model. (
  • Methods: We examined the effect of alpha lipoic acid alone or in combination with cisplatin and 5-fluorouracil on proliferation of the two cell lines, HeLa (human cervical carcinoma cells) and Caco-2 (human colon cancer cells) by MTT test. (
  • DADS anti-promoting effect may partly result from its ability to inhibit tumoral cell proliferation in vivo and in vitro. (
  • In the laboratory, apples have been found to have very strong antioxidant activity, inhibit cancer cell proliferation, decrease lipid oxidation, and lower cholesterol. (
  • Barrier function, migration, and proliferation were measured using electric cell-substrate impedance-sensing technology. (
  • Cell migration and proliferation during in vitro wound healing were inhibited. (
  • In line with other studies, we measured heterogeneity in terms of cell cycle, proliferation, and junction organisation. (
  • We correlated cell proliferation with different NOTCH activation states at the single cell and population levels. (
  • Lahiani MH, Gokulan K, Williams K, Khare S. Ex Vivo Human Colon Tissue Exposure to Pristine Graphene Activates Genes Involved in the Binding, Adhesion and Proliferation of Epithelial Cells. (
  • This study examines the anti-proliferative activity of T. carpentariae leaf and T. grandiflora leaf, fruit and nut extracts against Caco-2 and HeLa carcinoma proliferation. (
  • Materials and Methods: Powdered T. carpentariae leaf and T. grandiflora leaf, fruit and nut were extracted and tested for anti-proliferative activity against Caco-2 and HeLa cancer cell lines using colorimetric cell proliferation assays. (
  • The methanolic T. grandiflora leaf extract was particularly effective at blocking the proliferation of the colorectal carcinoma Caco-2 (IC50 = 372 μg/mL). (
  • The methanol T. carpentariae and T. grandiflora leaf extracts were similarly potent inhibitors of HeLa cervical cancer cell proliferation with IC50 values of 864 and 833 μg/mL respectively. (
  • Methods: A confluent monolayer of Caco-2 cells was pre-incubated with ALA (24 h) prior to control (37 °C) or HS conditions (42 °C) for 6 or 24 h and the expression of heat shock protein 70 (HSP70), heat shock factor-1 (HSF1), and the antioxidant Nrf2 were investigated. (
  • We report here the construction of an adenoviral vector that has the capability to be transported across polarized epithelial monolayers of Caco-2 cells (a colon carcinoma cell line) by transcytosis. (
  • ICC: MCF-7 cells, A2058 melanoma cells, mouse fibroblasts and HeLa cells. (
  • Results: At least one of the mechanisms of the antiproliferative and/or cytotoxic effect of alpha lipoic acid on Caco-2 and HeLa cells at high concentrations, the transcription factor NF-κB, may be involved, as well as the products of transcription of genes that are under its control. (
  • T. carpentariae leaf and T. grandiflora leaf, fruit and nut extracts displayed potent anti-proliferative activity against Caco-2 and HeLa carcinoma cells. (
  • Conclusion: The low toxicity and anti-proliferative activity observed with the T. carpentariae and T. grandiflora extracts against Caco-2 and HeLa indicate their potential for the prevention and treatment of some cancers. (
  • Results showed that legume extracts induced cytotoxicity in caco-2 cells in a dose-dependent manner. (
  • 2011). In vitro study of P-glycoprotein induction as an antidotal pathway to prevent cytotoxicity in Caco-2 cells . (
  • When cultured in this format, the cells differentiate to form a polarized epithelial cell monolayer that provides a physical and biochemical barrier to the passage of ions and small molecules. (
  • As dysbiosis has been reported in inflammatory bowel diseases (IBD), we aim to investigate the impact of IBD-associated dysbiosis on BA metabolism and its influence on the epithelial cell inflammation response. (
  • Inflammatory bowel disease is typically associated with a decrease in local pH, which may lead to altered epithelial barrier function and subsequent gastrointestinal repair involving epithelial cell adhesion and migration. (
  • electrophoretic-mobility-shift-assay analyses showed that the retarded complex due to Bbf-H promoter interaction is significantly increased in the differentiated cells. (
  • Well diffusion assays were conducted to determine the propensity of kefir isolates to inhibit growth of enteric pathogens, and a competitive adhesion/exclusion assay was used to determine the ability of kefir isolates to out-compete or exclude attachment of enteric pathogens to Caco-2 cells. (
  • 14. Twentyman PR, Luscombe M. A study of some variables in tetrazolium dye (MTT) based assay for cell growth and chemosensitivity. (
  • We aimed at evaluating its antiproliferative and antiangiogenic effects in human colon adenocarcinoma Caco‐2 cell line. (
  • This study examined the anti-tumor activity of the crude water extract of C. asiatica using human colon adenocarcinoma-derived Caco-2 cells. (
  • To elucidate the potential role of PKC-α on several phenotypic characteristics of colon cancer cells, we have transfected cDNAs for PKC-α in sense or antisense orientations into CaCo-2 cells, a human colonic adenocarcinoma cell line. (
  • Caco-2 (from Cancer coli, "colon cancer") is an immortalized cell line of human colorectal adenocarcinoma cells. (
  • Human colonic ADENOCARCINOMA cells that are able to express differentiation features characteristic of mature intestinal cells, such as ENTEROCYTES. (
  • Caco-2 cells were originally derived from human colon adenocarcinoma. (
  • Caco-2 cells are human colonic adenocarcinoma cells that form confluent, polarized epithelial monolayers with well-differentiated intercellular tight junctions structures. (
  • Desirable probiotic characteristics, including good survival in acid and bile environments, bile salt hydrolase activity, antioxidant activity, non-cytotoxicity and high adhesion to Caco-2 cells, and a lack of virulence or antimicrobial resistance genes. (
  • Dynamic Surfaces for the Study of Mesenchymal Stem Cell Growth through Adhesion Regulation ACS Nano. (
  • Cell adhesion to the extracellular matrix. (
  • Gene expression analysis revealed significant upregulation of genes related to cytoskeleton remodeling, cell adhesion, and growth factor signaling. (
  • The objective of this project was to evaluate the biological activity of peptides derived from the EC-4 domain of E-cadherin in inhibiting E-cadherin-mediated cell-cell adhesion. (
  • The results showed that peptide 6 (LVVQAADLQG) derived from the EC-2 domain and peptide 8 derived from the EC-4 domain (YTALIIATDN) of E-cadherin were more effective in inhibiting single cell adhesion than peptides from other domains (EC-1, EC-3) of E-cadherin. (
  • Alanine-scanning and mutation experiments found that peptide 22 (TEIIAT) had the best activity compared to other hexapeptides derived from peptide 8 in inhibiting cell adhesion of Caco-2 cells and junction resealing of MDCK-II cell monolayers. (
  • We found that breast cancer resistance protein (BCRP) was not involved in 7,8-DHF secretion and that the transport mechanism of 7,8-DHF was passive transport with an active efflux mediated by P-glycoprotein (P-gp) and multidrug resistance associated proteins (MRPs), particularly MRP 2. (
  • 2004. The dietary phytochemical chlorophyllin alters E-cadherin and beta-catenin expression in human colon cancer cells. . (
  • 2013. Epigenetic inactivation of endothelin-2 and endothelin-3 in colon cancer. . (
  • 2017. A functional pseudogene, NMRAL2P, is regulated by Nrf2 and serves as a coactivator of NQO1 in sulforaphane-treated colon cancer cells. . (
  • Therefore, in colon cancer cells, GSDME mediates lobaplatin-induced pyroptosis downstream of the ROS/JNK/Bax-mitochondrial apoptotic pathway and caspase-3/-9 activation. (
  • Myeloma cancer cell responding to shear flow (by scanning electron microscope). (
  • A new function for the serine protease HtrA2 in controlling radiation-induced senescence in cancer cells. (
  • The Serine Protease HtrA2/Omi mediates radiation-induced senescence in lung cancer cells. (
  • The compound exhibited distinct cytotoxicities against cancer cell lines A-549, HL-60, BEL-7402, and P388. (
  • This derivatization (from compound 7 to compound 15) results in an average GI(50) value of 1.98 mu M in the DTP (NCI-60) cell panel, resulting in being especially active in renal cancer cells. (
  • Some of these selenium derivatives demonstrated multidrug resistance (MDR)-reversing activity in different resistant cancer cell lines. (
  • Three separate research groups have confirmed that these chemicals have significant antitumorous properties and selective toxicity against various types of cancer cells (without harming healthy cells) publishing eight clinical studies on their findings. (
  • This is why they are toxic to cancer cells but have no toxicity to healthy cells. (
  • Colon cancer growth requires growth-promoting connections between malignant colonocytes and stromal cells. (
  • Intro Colon cancer growth is driven by cell-cell and cell-matrix physical relationships and paracrine and autocrine signals including malignant colonocytes and assisting stromal cells. (
  • Colon cancer stroma is progressively recognized as playing an active part in colonic tumor development (1 Bioymifi 2 The stroma includes fibroblasts immune cells endothelial cells and the extracellular matrix which communicate stimulatory and inhibitory cues to tumor epithelial cells via complex networks (1 2 Growth factors cytokines chemokines prostanoids integrins and additional bioactive molecules mediate these bidirectional signals. (
  • For studies we used parental HCT116 colon cancer cells and exploited a mouse expressing in order to abrogate EGFR signals in the tumor stroma (19 20 To dissect the contribution of colon cancer cell EGFR to tumor xenograft growth we bioengineered HCT116 cells to express a dominant bad EGFR (DN-EGFR) under doxycycline-inducible (rtTA) rules. (
  • Unlike Bioymifi in stromal cells or colon cancer cells to dissect cell- or compartment-specific EGFR contributions to cell signals and tumor xenograft growth. (
  • For these studies we also examined the effects of stromal cell and colon cancer cell EGFR on pro-inflammatory interleukin 1 beta (IL1B) that is upregulated in colon cancer and has been shown to induce EGFR ligands in colonic fibroblasts (5 JUN 21 To dissect how EGFR and IL1B signals interact and crosstalk between malignancy cells and stromal cells we used mono- and coculture models. (
  • To determine how colon cancer cells modulate PTGS2 manifestation in stromal fibroblast cells we used a novel strategy including fibroblasts cocultured with colon cancer cells that indicated an inducible DN-EGFR. (
  • In the case of colon cancer cells we transfected Caco-2 cells. (
  • Accounting for nearly 10 million deaths in 2020 worldwide, it is expected that the total number of cancer cases will rise from 19.3 million in 2020 to 30.2 million in 2040 [ 1 , 2 ]. (
  • So far, the investigation in cancer cell lines of the modulation of cancer growth and progression by oxysterols, in particular 27-hydroxycholesterol (27HC), has yielded controversial results. (
  • Primary central nervous system (CNS) malignancies comprise 1-to-2 per cent of all new cancer diagnoses in Ireland. (
  • However, in vitro and in vivo studies confirm also the cytostatic and cytotoxic effects of statins against various types of cancer cells including colon cancer. (
  • It is a multi-step process which is initiated by invasion of the peritumoral stroma by cancer cells and which leads to the dissemination of cancer cells in the organism.My PhD work aimed at identifying the molecular and cellular process driving colorectal carcinoma (CRC) invasion, which is the 2nd most frequent cancer worldwide. (
  • Our analysis of live and human primary cancer specimen revealed that CRC cells used a collective mode of invasion to disseminate, in which cells retain an epithelium specific -glandular architecture. (
  • PET-cGMP-8-TMAmd-(PEG pd 2000)-AmdMT-8-cGMP-PET decreased cell viability in the PKG2 expressing colon cancer cell line Caco-2, but not in the PKG2 expressing colon cancer cell lines HCT 116 and HT-29. (
  • Anticancer effects of carboxymethylated (1→3)(1→6)-β-D-glucan (botryosphaeran) on multicellular tumor spheroids of MCF-7 cells as a model of breast cancer. (
  • The mRNA expression level of Bcl-2 and p53 was significantly decreased and increased respectively in cancer cells treated by luteolin. (
  • Tyvelose-specific antibodies exclude larvae from monolayers of usually prone Madin-Darby canine kidney (MDCK) cells (16). (
  • The Caco-2 monolayer is widely used across the pharmaceutical industry as an in vitro model of the human small intestinal mucosa to predict the absorption of orally administered drugs with different kits such as the CacoReady. (
  • The bioactivity of trans-resveratrol (RSV), an important wine polyphenol, and of its metabolites was investigated in a more relevant setup comprising an in vitro coculture cell model that combines intestinal absorption and conjugation with changes in endothelial function, which is primarily affected in cardiovascular diseases. (
  • Using the Caco-2 cell model to simulate the human absorption process, the bioavailable contents and the bioavailability of the trace elements were further determined. (
  • Presently, alpha-tocopherol is often combined with other antioxidants and other forms of vitamin E. An in vitro study showed that the polyphenol naringenin and a carotenoids mixture (lycopene, beta-carotene and lutein) significantly impaired the absorption of alpha-tocopherol across a cell system model of the intestine (Caco-2 cells). (
  • In vitro studies to assess a drug's benefits and side effects by investigating the drug's activities(cell absorption, distribution, metabolism and excretion). (
  • The purpose of this study was to investigate the intestinal epithelial absorption of eckols using Caco-2 cell monolayers of the small intestinal membrane model. (
  • An Openable Artificial Intestinal Tract System Enables the Evaluation of Drug Absorption in Caco-2 Cells through the Reduction in Thickness of the Unstirred Water Layer. (
  • The LS AFM is used in life sciences applications when an inverted optical microscope is required for locating cells or other bio-materials on a surface. (
  • The differentiation process of these cells in continuous culture is accompanied by an accumulation of the mRNA coding for the apoferritin H chain. (
  • The H gene transcriptional activation seems to be a specific feature of differentiated Caco-2 cells, since the activity of other promoters did not change upon differentiation. (
  • In cellular biology convergence, this rare property in fluids has been leveraged to better study complex cell behaviors, such as cell motility in response to chemotactic stimuli , stem cell differentiation , axon guidance , subcellular propagation of biochemical signaling and embryonic development . (
  • 3D cell-culture models exceed 2D culture systems by promoting higher levels of cell differentiation and tissue organization. (
  • The analysis of Caco-2 subclones stably transfected with an H-chain promoter-chloramphenicol acetyltransferase (CAT) construct revealed that the mRNA increase is paralleled by an enhanced transcription of the H gene, driven by the -100 to +4 region of the H promoter. (
  • Caco-2 cells stably overexpressing OGR1 were created and validated as tools to study OGR1 signaling. (
  • Generation of Caco-2 cells stably expressing CYP3A4·POR·UGT1A1 and CYP3A4·POR·UGT1A1*6 using a PITCh system. (
  • Clearance of small intestinal crypts involves goblet cell mucus secretion by intracellular granule rupture and enterocyte ion transport. (
  • The extract also increased the accumulation of cyclin B1 protein in the cells. (
  • These active compounds were also effective in colon carcinoma Caco-2 cells, and some of them increased the expression of p62 protein, a typical substrate of autophagic proteolysis, indicating that phytochemicals impact p62 levels in autophagy-dependent and/or -independent manners. (
  • Further, experimental validation showed that the QX1 formula inhibited the activity of calcium/calmodulin-dependent protein kinase II (CaMKII), MAPK (P38, ERK1/2, and JNK), and TLR4/NF- κ B signaling pathways but promoted the activation of the PI3K/AKT pathway. (
  • The receptor-binding domain (RBD) in S1 subunit and heptad repeat 1 (HR1) domain in S2 subunit of SARS-CoV-2 spike (S) protein are the targets of neutralizing antibodies (nAbs) and pan-coronavirus (CoV) fusion inhibitory peptides, respectively. (
  • Mechanistic studies suggest that S-20-1 binds with high affinity to RBD in S1 and HR1 domain in S2 of SARS-CoV-2 S protein. (
  • Thus, with its pan-CoV fusion and entry inhibitory activity by targeting two sites in S protein, desirable half-life, and promising oral bioavailability, S-20-1 is a potential candidate for further development as a novel therapeutic and prophylactic drug against infection by SARS-CoV-2 and its variants, as well as future emerging and reemerging CoVs. (
  • To date, several strategies have been adopted for the development of anti-SARS-CoV-2 therapeutics by targeting viral spike (S) protein (S1 and S2 subunits), viral enzymes (PLpro, 3 CLpro, RdRp and helicase) 13 , and some structure proteins 13 . (
  • Jiang's group previously identified a series of pan-CoV fusion inhibitors, such as EK1 peptide and EK1C4 lipopeptide, targeting the heptad repeat 1 (HR1) domain in S2 subunit of SARS-CoV-2 S protein with highly potent antiviral activity against all HCoVs tested 22 , 23 , demonstrating the potential of using S protein to develop pan-antiviral inhibitors. (
  • AIMS: To study effects on cellular innate immune responses to ORF8, ORF10, and Membrane protein (M protein) from the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that causes COVID-19, in combination with cannabidiol (CBD). (
  • We also observed that DADS induced an increase in p21(waf1/cip1) expression, at mRNA and protein levels, in both cell lines. (
  • Currently, I am focus on integrating single cell RNA and protein expression data to develop models to predict patient at risk for Antigen Loss relapse after CAR T cells immunotherapy. (
  • 2 Alpha-gal syndrome stands apart from conventional, IgE-mediated food allergies because the IgE driving the allergy forms against a sugar (alpha-gal) rather than a protein. (
  • Treatment with the protein kinase A inhibitor H89 or the anion exchange inhibitor 4,4'-diisothiocyano-2,2'-stilbenedisulfonic acid completely blocked the CM-induced oxalate transport. (
  • Nicotinic acid is important for cell respiration and metabolism of carbohydrate, fat and protein. (
  • Relatively high concentrations of this oxysterol markedly increased the release of pro-inflammatory interleukins 6 and 8, monocyte chemoattractant protein-1, vascular endothelial growth factor, as well as matrix metalloproteinases 2 and 9. (
  • 8. Youle RJ, Strasser A. The BCL-2 protein family: opposing activities that mediate cell death. (
  • One study identified a genetic polymorphism of the human plasma protein haptoglobin, Hp 2, which may be an important non-nutritional modifying factor in the pathogenesis of vitamin C deficiency. (
  • Porcine aortic endothelial cell, double-labeled for actin (green) and phospho-tyrosine (red). (
  • Although derived from a colon (large intestine) carcinoma, when cultured under specific conditions the cells become differentiated and polarized such that their phenotype, morphologically and functionally, resembles the enterocytes lining the small intestine. (
  • This could probably be due to the fact that the epithelial lining of the large and small intestine typically comprises a heterogenous mixture of enterocytes, enteroendocrine cells, goblet cells, transit amplifying cells, paneth cells and intestinal stem cells. (
  • In this study we examined immune response of Caco-2 enterocytes after incubation with common probiotic Bifidobacterium animalis subsp. (
  • The aim of this study was to determine whether multiwalled carbon nanotube s (MWNCT) are taken up by and are toxic to human intestinal enterocytes using the Caco-2 cell model. (
  • Cells displayed many of the properties that characterize enterocytes, such as apical microvilli, basolateral basement membrane, and glycogen. (
  • Our study suggests that intestinal enterocytes cells do not take up MWCNT. (
  • Despite their origin, Caco-2 cells grow in culture to form a polarised monolayer with tight junctions and an apical brush border that differentiate on a semi-permeable membrane that displays similar morphological and functional characteristics as small intestinal enterocytes. (
  • In the SMALL INTESTINE, the mucosa is characterized by a series of folds and abundance of absorptive cells (ENTEROCYTES) with MICROVILLI. (
  • these to start an infection by invading columnar epithelial cells in the tiny intestine. (
  • In summary, we demonstrate that residues in the N1 subdomain are required for export and cell wall localization of S. aureus MSCRAMM proteins. (
  • Regulation of expression and activity of multidrug resistance proteins MRP2 and MDR1 by estrogenic compounds in Caco-2 cells. (
  • Moreover, the spectroscopic results for colon normal cells and cancerous cells based on data typical for nucleic acids, proteins, lipids have been compared. (
  • Pflügers Archiv: European Journal of Physiology 2001, 443 (2), 265-273. (
  • The convergence of labs-on-chips (LOCs) and cell biology has permitted the study of human physiology in an organ-specific context, introducing a novel model of in vitro multicellular human organisms. (
  • Journal of Comparative Physiology B. 164 , 2 , p. 159-164 6 p. (
  • Desde 2010, Editor-in-Chief del Journal of Physiology and Biochemistry (, revista científica de la Universidad de Navarra, fundada en 1945 por los Drs. Juan Jiménez Vargas y Francisco Ponz, y publicada desde el 2010 por la editorial Springer-Nature. (
  • We conclude that acidic extracellular pH can have a signaling function and impact the physiology of intestinal epithelial cells. (
  • American Journal of Physiology - Cell Physiology , 296 (1), C13-C24. (
  • It inhibits glucose 6-phosphate dehydrogenase(G6PD) in human tumor cells, binds hemoglobin and drives RBC-mediated redox reactions under hypoxia. (
  • Devimistat (CPI-613), a lipoate analog, inhibits mitochondrial enzymes pyruvate dehydrogenase (PDH) and α-ketoglutarate dehydrogenase in NCI-H460 cell line, disrupts tumor cell mitochondrial metabolism. (
  • Diallyl disulfide (DADS) increases histone acetylation and p21(waf1/cip1) expression in human colon tumor cell lines. (
  • As far as DADS may modulate the expression of a subset of genes, we investigated DADS effect on histone acetylation, in two human colon tumor cell lines. (
  • I hypothesize that resistant tumor cells, and their features associated with CD19Neg relapse, are present before CAR-T administration and can be detected and used to build a model to predict which patients are at risk for CD19Neg relapse. (
  • Many of the acetogenins have demonstrated selective toxicity to tumor cells at very low dosages-as little as 1 part per million. (
  • Mode of action studies in three separate laboratories have recently determined that these acetogenins are superb inhibitors of enzyme processes that are only found in the membranes of cancerous tumor cells. (
  • Stromal cell and colonocyte EGFRs are necessary for strong EGFR signals and efficient tumor growth which involve EGFR-interleukin-1 Bioymifi crosstalk. (
  • To handle the efforts of colonocyte and stromal cell EGFR to tumor development Bioymifi we used tumor xenograft models and coculture models to dissect cell-specific tasks of EGFR. (
  • To investigate possible pro-tumor properties of 27HC, its effects were studied in vitro in differentiated CaCo-2 cells. (
  • It ranges from a well-differentiated tumor with EPITHELIAL CELLS indistinguishable from normal HEPATOCYTES to a poorly differentiated neoplasm. (
  • The Antitumoral/Antimetastatic Action of the Flavonoid Brachydin A in Metastatic Prostate Tumor Spheroids In Vitro Is Mediated by (Parthanatos) PARP-Related Cell Death. (
  • Cell Biology and Toxicology. (
  • In: Ralph A. Bradshaw and Philip D. Stahl (Editors-in-Chief), Encyclopedia of Cell Biology. (
  • In Vitro Cell and Developmental Biology Plant. (
  • Molecular biology of the cell. (
  • A sound understanding of culture behavior, cell biology and genetics has to be combined with bioreactor technology, tailored analysis, monitoring and control strategies. (
  • In this paper, we examine the mechanisms that regulate the expression of the heavy (H) ferritin subunit in the colon carcinoma Caco-2 cell line allowed to differentiate spontaneously in vitro. (
  • P-gp inhibitory effect of aqueous extracts (250 µg/mL) from some medicinal plants and verapamile (5 µg/mL) was measured using flow cytometry by Rhodamine 123 (Rh123) in Caco2 cell line. (
  • The current study aims to understand the safety aspects of AN-PEP hydrolysed low immunoreactive wheat flours by testing immune responses using cell line and animal models. (
  • We herein demonstrated that once PtdSer was exposed on the cell surface of ATP11A −/− ATP11C −/− mouse T cell line (W3), its internalization to the inner leaflet of plasma membranes was negligible at 15 °C. (
  • We propose that the activation of H-ferritin gene expression may be associated with the establishment of a differentiated phenotype in Caco-2 cells, and that the H-ferritin gene transcriptional upregulation is accompanied by a modification in the activity of the transcription factor Bbf. (
  • Expression data from Caco-2 cells co-cultivated with B. animalis subsp. (
  • 2003. Mutational analysis of Ctnnb1 and Apc in tumors from rats given 1,2-dimethylhydrazine or 2-amino-3-methylimidazo[4,5-f]quinoline: mutational 'hotspots' and the relative expression of beta-catenin and c-jun. . (
  • In cells expressing viral genes, CBD augmented expression of IFNγ, IFNλ1 and IFNλ2/3, as well as the 2'-5'-oligoadenylate synthetase (OAS) family members OAS1, OAS2, OAS3, and OASL. (
  • Relative ABCA1 mRNA levels were normalized to beta-actin and their relative expression to untreated cells was analyzed using delta-delta CT method. (
  • In Caco-2 cells, expression of the apoAI gene and activation by the distal enhancer required the presence of a nuclear hormone receptor response element (NHRRE) located in the -214 to -192 apoAI promoter region. (
  • Increasing cell density had no effect on Sp1/Sp3 expression but resulted in their increased binding to the SpA/B/C probe along with Stat3 and concurrently with enhanced nuclear pTyr 705 -Stat3 level. (
  • Thus, during confluency, ligand-independent Stat3 activation leads to its interaction with Sp1/Sp3, their recruitment to the SpA/B/C cluster in a Stat3 DNA-binding domain-dependent fashion, increased transcription, and expression of NHE3, to coordinate cell density-mediated epithelial dome formation. (
  • Cao X, Khare S, DeLoid GM, Gokulan K, Demokritou P. Co-exposure to boscalid and TiO2 (E171) or SiO2 (E551) downregulates cell junction gene expression in small intestinal epithelium cellular model and increases pesticide translocation. (
  • This study aimed to compare three different extracts of Saco sweet cherry, namely non-coloured fraction, coloured fraction and total extract concerning phenolic composition, antioxidant and antidiabetic potential, erythrocytes' protection and effects on Caco-2 cells. (
  • A 20-80% increase in release of LDH was observed in cells incubated with extracts compared to untreated cells. (
  • Total fragmented DNA (%) was higher in cells treated with sprouted legume extracts compared to their non-sprouted extracts. (
  • Immunoprecipitation performed with the nuclear extracts demonstrated physical interaction of Stat3 and Sp1/Sp3 triggered by cell confluency. (
  • An N-terminal signal sequence directs export of the MSCRAMM by the Sec pathway and the C-terminal cell wall-anchoring domain allows covalent attachment of ClfA to peptidoglycan by sortase. (
  • On the other hand, foam cell homeostasis is regulated by the RCT pathway. (
  • Growth in normal and tumour cells is regulated by evolutionarily conserved extracellular inputs from the endocrine insulin receptor (InR) signalling pathway and by local nutrients. (
  • The Hp 2-2 phenotype is present in 35% of whites and 50% of South Asians and East Asians and may help identify patients who are more prone to develop clinically significant vitamin C deficiency. (
  • Moreover, the use of various influx transporter inhibitors in Caco-2 cells showed that organic cation transporters (OCTs) and organic anion-transporting polypeptides (OATPs) participated in 7,8-DHF transport. (
  • The Hp 2-2 polymers are less efficient inhibitors of hemoglobin-driven oxidative stress, leading to ascorbic acid depletion. (
  • Over the years, the characteristics of the cells used in different laboratories around the world have diverged significantly, which makes it difficult to compare results across labs. (
  • The Pgp-mediated transport of DPC across Caco-2 cells was readily saturated at ≥10 μM and was inhibited significantly by RTV at 5 to 10 μM. (
  • The polyphenol intervention significantly reduced the levels of VEGF, ROS, IL-8 and ICAM-1, with a more pronounced effect in TNF-alpha-activated endothelial cells. (
  • The Zo-1 localization in Caco-2 cells was significantly noted in the reacted positive fluorescence cells that were treated with the control wheat flour. (
  • Kemtrace Zn MP had the significantly higher serum zinc level than zinc sulphate group, Table 2 . (
  • In addition, the phosphorylation of STAT3 was significantly downregulated by CBD and THC in THP-1 macrophages and HBECs, which was in turn attributed to the reduced phosphorylation of tyrosine kinase-2 (TYK2) by CBD and THC after LPS stimulation in these cells. (
  • amphiregulin (AREG) PTGS2 and and interleukin-1 receptor 1 transcripts and cancers cell beta catenin (CTNNB1) and cyclin D1 (CCND1) had been significantly low in tumors extracted from mice. (
  • Naunyn-Schmiedeberg's Archives of Pharmacology, 372 (2), 147 - 152. (
  • The toxicity of cleaved PT-gliadin products was assessed in Caco-2 epithelial cells, celiac patient-derived T cells, and in human small intestinal mucosal organ culture biopsies. (
  • In the AN-PEP hydrolysed wheat flour (AN-PEP HWF) gliadin extract, there was no increase in the levels of zonulin-1 (Zo-1) and pro-inflammatory cytokines (IL-6 and IL-8) but a significant increase was noted in the control wheat flour (CWF) gliadin-treated Caco-2 cells. (
  • In this study, we investigated the effect of camu-camu on nonheme iron bioavailability from two different meals (rice with lentils and wheat flour porridge) using an in vitro Caco-2 cell model. (
  • Caco-2 cells are most commonly used not as individual cells, but as a confluent monolayer on a cell culture insert filter (e.g. (
  • Regarding to Caco-2 cells, the coloured extract exhibited the most cytotoxic effects, while the total extract was the most efficient in protecting these cells against oxidative damage induced by t-BHP. (
  • Cytotoxic and apoptogenic activity of a methanolic extract from the marine invertebrate Ciona intestinalis on malignant cell lines. (
  • GC-MS analysis of the methanolic T. grandiflora leaf extract identified 3 lanostane and 2 pentacyclic triterpenoids. (