Calcium Carbonate: Carbonic acid calcium salt (CaCO3). An odorless, tasteless powder or crystal that occurs in nature. It is used therapeutically as a phosphate buffer in hemodialysis patients and as a calcium supplement.Caco-2 Cells: Human colonic ADENOCARCINOMA cells that are able to express differentiation features characteristic of mature intestinal cells, such as ENTEROCYTES. These cells are valuable in vitro tools for studies related to intestinal cell function and differentiation.Haptophyta: A group (or phylum) of unicellular EUKARYOTA (or algae) possessing CHLOROPLASTS and FLAGELLA.Pinctada: A genus of pearl oysters in the family Pteriidae, class BIVALVIA. Both cultured and natural pearls are obtained from species in the genus. They are distinct from the distantly related, edible true oysters of the family OSTREIDAE.Lithostathine: The proteinaceous component of the pancreatic stone in patients with PANCREATITIS.Isopoda: One of the largest orders of mostly marine CRUSTACEA, containing over 10,000 species. Like AMPHIPODA, the other large order in the superorder Peracarida, members are shrimp-like in appearance, have sessile compound eyes, and no carapace. But unlike Amphipoda, they possess abdominal pleopods (modified as gills) and their bodies are dorsoventrally flattened.Calcification, Physiologic: Process by which organic tissue becomes hardened by the physiologic deposit of calcium salts.Intestinal Mucosa: Lining of the INTESTINES, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. In the SMALL INTESTINE, the mucosa is characterized by a series of folds and abundance of absorptive cells (ENTEROCYTES) with MICROVILLI.Carbonates: Salts or ions of the theoretical carbonic acid, containing the radical CO2(3-). Carbonates are readily decomposed by acids. The carbonates of the alkali metals are water-soluble; all others are insoluble. (From Grant & Hackh's Chemical Dictionary, 5th ed)SvalbardColonic Neoplasms: Tumors or cancer of the COLON.HT29 Cells: Human colonic ADENOCARCINOMA cells that are able to express differentiation features characteristic of mature intestinal cells such as the GOBLET CELLS.Oceans and Seas: A great expanse of continuous bodies of salt water which together cover more than 70 percent of the earth's surface. Seas may be partially or entirely enclosed by land, and are smaller than the five oceans (Atlantic, Pacific, Indian, Arctic, and Antarctic).Antigens, CD98: A heterodimeric protein that is a cell surface antigen associated with lymphocyte activation. The initial characterization of this protein revealed one identifiable heavy chain (ANTIGENS, CD98 HEAVY CHAIN) and an indeterminate smaller light chain. It is now known that a variety of light chain subunits (ANTIGENS, CD98 LIGHT CHAINS) can dimerize with the heavy chain. Depending upon its light chain composition a diverse array of functions can be found for this protein. Functions include: type L amino acid transport, type y+L amino acid transport and regulation of cellular fusion.Intestines: The section of the alimentary canal from the STOMACH to the ANAL CANAL. It includes the LARGE INTESTINE and SMALL INTESTINE.Seawater: The salinated water of OCEANS AND SEAS that provides habitat for marine organisms.Flounder: Common name for two families of FLATFISHES belonging to the order Pleuronectiformes: left-eye flounders (Bothidae) and right-eye flounders (Pleuronectidae). The latter is more commonly used in research.Antacids: Substances that counteract or neutralize acidity of the GASTROINTESTINAL TRACT.Cephalexin: A semisynthetic cephalosporin antibiotic with antimicrobial activity similar to that of CEPHALORIDINE or CEPHALOTHIN, but somewhat less potent. It is effective against both gram-positive and gram-negative organisms.Symporters: Membrane transporters that co-transport two or more dissimilar molecules in the same direction across a membrane. Usually the transport of one ion or molecule is against its electrochemical gradient and is "powered" by the movement of another ion or molecule with its electrochemical gradient.Anthozoa: A class in the phylum CNIDARIA, comprised mostly of corals and anemones. All members occur only as polyps; the medusa stage is completely absent.Calcium, Dietary: Calcium compounds used as food supplements or in food to supply the body with calcium. Dietary calcium is needed during growth for bone development and for maintenance of skeletal integrity later in life to prevent osteoporosis.Atmosphere: The gaseous envelope surrounding a planet or similar body. (From Random House Unabridged Dictionary, 2d ed)Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Phosphorus: A non-metal element that has the atomic symbol P, atomic number 15, and atomic weight 31. It is an essential element that takes part in a broad variety of biochemical reactions.Invertebrates: Animals that have no spinal column.Calcium: A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.Plankton: Community of tiny aquatic PLANTS and ANIMALS, and photosynthetic BACTERIA, that are either free-floating or suspended in the water, with little or no power of locomotion. They are divided into PHYTOPLANKTON and ZOOPLANKTON.Intestinal Absorption: Uptake of substances through the lining of the INTESTINES.Carbon Dioxide: A colorless, odorless gas that can be formed by the body and is necessary for the respiration cycle of plants and animals.Parathyroid Hormone: A polypeptide hormone (84 amino acid residues) secreted by the PARATHYROID GLANDS which performs the essential role of maintaining intracellular CALCIUM levels in the body. Parathyroid hormone increases intracellular calcium by promoting the release of CALCIUM from BONE, increases the intestinal absorption of calcium, increases the renal tubular reabsorption of calcium, and increases the renal excretion of phosphates.Bicarbonates: Inorganic salts that contain the -HCO3 radical. They are an important factor in determining the pH of the blood and the concentration of bicarbonate ions is regulated by the kidney. Levels in the blood are an index of the alkali reserve or buffering capacity.Biological Availability: The extent to which the active ingredient of a drug dosage form becomes available at the site of drug action or in a biological medium believed to reflect accessibility to a site of action.Phosphates: Inorganic salts of phosphoric acid.Colon: The segment of LARGE INTESTINE between the CECUM and the RECTUM. It includes the ASCENDING COLON; the TRANSVERSE COLON; the DESCENDING COLON; and the SIGMOID COLON.PolyaminesEpithelial Cells: Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.Hyperparathyroidism, Secondary: Abnormally elevated PARATHYROID HORMONE secretion as a response to HYPOCALCEMIA. It is caused by chronic KIDNEY FAILURE or other abnormalities in the controls of bone and mineral metabolism, leading to various BONE DISEASES, such as RENAL OSTEODYSTROPHY.Hypercalcemia: Abnormally high level of calcium in the blood.Tumor Cells, Cultured: Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.Geologic Sediments: A mass of organic or inorganic solid fragmented material, or the solid fragment itself, that comes from the weathering of rock and is carried by, suspended in, or dropped by air, water, or ice. It refers also to a mass that is accumulated by any other natural agent and that forms in layers on the earth's surface, such as sand, gravel, silt, mud, fill, or loess. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed, p1689)Crystallization: The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Cell Polarity: Orientation of intracellular structures especially with respect to the apical and basolateral domains of the plasma membrane. Polarized cells must direct proteins from the Golgi apparatus to the appropriate domain since tight junctions prevent proteins from diffusing between the two domains.Microscopy, Electron, Scanning: Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Jejunum: The middle portion of the SMALL INTESTINE, between DUODENUM and ILEUM. It represents about 2/5 of the remaining portion of the small intestine below duodenum.Biological Transport: The movement of materials (including biochemical substances and drugs) through a biological system at the cellular level. The transport can be across cell membranes and epithelial layers. It also can occur within intracellular compartments and extracellular compartments.Alkaline Phosphatase: An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.1.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Fermentation: Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.Epithelium: One or more layers of EPITHELIAL CELLS, supported by the basal lamina, which covers the inner or outer surfaces of the body.Ecosystem: A functional system which includes the organisms of a natural community together with their environment. (McGraw Hill Dictionary of Scientific and Technical Terms, 4th ed)Cell Line: Established cell cultures that have the potential to propagate indefinitely.Cell Line, Tumor: A cell line derived from cultured tumor cells.Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Magnesium: A metallic element that has the atomic symbol Mg, atomic number 12, and atomic weight 24.31. It is important for the activity of many enzymes, especially those involved in OXIDATIVE PHOSPHORYLATION.Sodium: A member of the alkali group of metals. It has the atomic symbol Na, atomic number 11, and atomic weight 23.Bone and Bones: A specialized CONNECTIVE TISSUE that is the main constituent of the SKELETON. The principle cellular component of bone is comprised of OSTEOBLASTS; OSTEOCYTES; and OSTEOCLASTS, while FIBRILLAR COLLAGENS and hydroxyapatite crystals form the BONE MATRIX.Colorectal Neoplasms: Tumors or cancer of the COLON or the RECTUM or both. Risk factors for colorectal cancer include chronic ULCERATIVE COLITIS; FAMILIAL POLYPOSIS COLI; exposure to ASBESTOS; and irradiation of the CERVIX UTERI.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Adenocarcinoma: A malignant epithelial tumor with a glandular organization.Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Gene Expression Regulation, Neoplastic: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue.Reverse Transcriptase Polymerase Chain Reaction: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Gene Expression: The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.Kinetics: The rate dynamics in chemical or physical systems.

Cell surface-associated lipoteichoic acid acts as an adhesion factor for attachment of Lactobacillus johnsonii La1 to human enterocyte-like Caco-2 cells. (1/3466)

The influence of pH on the adhesion of two Lactobacillus strains to Caco-2 human intestinal cells was investigated. One strain, Lactobacillus johnsonii La1, was adherent at any pH between 4 and 7. The other one, L. acidophilus La10, did not attach to this cell line under the same experimental conditions. On the basis of these results, we used the monoclonal antibody technique as a tool to determine differences on the surface of these bacteria and to identify a factor for adhesion. Mice were immunized with live La1, and the hybridomas produced by fusion of spleen cells with ONS1 cells were screened for the production of antibodies specific for L. johnsonii La1. A set of these monoclonal antibodies was directed against a nonproteinaceous component of the L. johnsonii La1 surface. It was identified as lipoteichoic acid (LTA). This molecule was isolated, chemically characterized, and tested in adhesion experiments in the same system. The adhesion of L. johnsonii La1 to Caco-2 cells was inhibited in a concentration-dependent way by purified LTA as well as by L. johnsonii La1 culture supernatant that contained LTA. These results showed that the mechanism of adhesion of L. johnsonii La1 to human Caco-2 cells involves LTA.  (+info)

Cytochrome P-450 1A1 expression in human small bowel: interindividual variation and inhibition by ketoconazole. (2/3466)

Human cytochrome P-450 1A1 (CYP1A1) is located primarily in extrahepatic tissues. To begin the characterization of this enzyme in the small intestine, we screened a bank of 18 human small intestinal microsomal preparations for CYP1A1 catalytic [(7-ethoxyresorufin O-deethylase (EROD)] activity and protein content. Although EROD activity was below detectable limits in 12 of the preparations, 6 exhibited measurable activity (1.4-123.5 pmol/min/mg), some exceeding that for 2 human liver microsomal preparations (11.0 and 26.4 pmol/min/mg). This variation was not due to variable quality of the preparations because each sample displayed readily detectable CYP3A4 catalytic activity and immunoreactive protein. We inadvertently found that intestinal EROD activity was inhibitable by ketoconazole at a concentration commonly believed to selectively inhibit CYP3A4. The possibility that CYP3A4 metabolizes 7-ethoxyresorufin was excluded because there was no correlation between intestinal CYP3A4 catalytic and EROD activity, and cDNA-expressed human CYP3A4 exhibited no EROD activity. Moreover, CYP1A1 immunoreactive protein was most abundant in the three intestinal preparations with the highest EROD activities, and the mean apparent Ki of ketoconazole observed for these three preparations (40 nM) was essentially identical with that for cDNA-expressed human CYP1A1 (37 nM). In summary, there is large interindividual variation in CYP1A1 expression in human small bowel, and ketoconazole is not a selective CYP3A4 inhibitor in in vitro metabolism studies involving intestinal tissue obtained from some individuals. These observations raise the possibility that in vivo drug interactions involving ketoconazole could result from CYP1A1 inhibition in the intestine in some individuals.  (+info)

CREB-binding [corrected] protein interacts with the homeodomain protein Cdx2 and enhances transcriptional activity. (3/3466)

Cdx2 encodes for a homeodomain protein that is expressed in intestinal epithelial cells. The Cdx2 protein triggers intestinal differentiation in cell lines and is necessary for maintenance of the intestinal phenotype in mice. CBP (cAMP response element-binding protein) is a transcriptional co-activator that interacts with many transcription factors and components of the basal transcriptional machinery. In this study, we demonstrate that CBP is markedly induced upon differentiation of the Caco-2 intestinal cell line and augments Cdx2-dependent transcriptional activity. Cdx2 interacts with the amino-terminal domain of CBP, and the two proteins coexist in vivo within the same nuclear protein complex. Moreover, expression of the CBP domain that interacts with Cdx2 acts as a dominant-negative inhibitor of transcriptional activation by Cdx2. These findings demonstrate a direct interaction between an intestinal homeodomain protein and CBP and suggest that CBP participates in the network of transcriptional proteins that direct intestinal differentiation.  (+info)

Regulation of the human apolipoprotein AIV gene expression in transgenic mice. (4/3466)

The apolipoprotein (Apo) AI-CIII-AIV gene cluster has a complex pattern of gene expression that is modulated by both gene- and cluster-specific cis-acting elements. In particular the regulation of Apo AIV expression has been previously studied in vivo and in vitro including several transgenic mouse lines but a complete, consistent picture of the tissue-specific controls is still missing. We have analysed the role of the Apo AIV 3' flanking sequences in the regulation of gene expression using both in vitro and in vivo systems including three lines of transgenic mice. The transgene consisted of a human fragment containing 7 kb of the 5' flanking region, the Apo AIV gene itself and 6 kb of the 3' flanking region (-7+6 Apo AIV). Accurate analysis of the Apo AIV mRNA levels using quantitative PCR and Northern blots showed that the 7+6 kb Apo AIV fragment confers liver-specific regulation in that the human Apo AIV transgene is expressed at approximately the same level as the endogenous mouse Apo AIV gene. In contrast, the intestinal regulation of the transgene did not follow, the pattern observed with the endogenous gene although it produced a much higher intestinal expression following the accepted human pattern. Therefore, this animal model provides an excellent substrate to design therapeutic protocols for those metabolic derangements that may benefit from variations in Apo AIV levels and its anti-atherogenic effect.  (+info)

Epidermal growth factor regulates fatty acid uptake and metabolism in Caco-2 cells. (5/3466)

Epidermal growth factor (EGF) has been reported to stimulate carbohydrate, amino acid, and electrolyte transport in the small intestine, but its effects on lipid transport are poorly documented. This study aimed to investigate EGF effects on fatty acid uptake and esterification in a human enterocyte cell line (Caco-2). EGF inhibited cell uptake of [14C]palmitate and markedly reduced its incorporation into triglycerides. In contrast, the incorporation in phospholipids was enhanced. To elucidate the mechanisms involved, key steps of lipid synthesis were investigated. The amount of intestinal fatty acid-binding protein (I-FABP), which is thought to be important for fatty acid absorption, and the activity of diacylglycerol acyltransferase (DGAT), an enzyme at the branch point of diacylglycerol utilization, were reduced. EGF effects on DGAT and on palmitate esterification occurred at 2-10 ng/ml, whereas effects on I-FABP and palmitate uptake occurred only at 10 ng/ml. This suggests that EGF inhibited palmitate uptake by reducing the I-FABP level and shifted its utilization from triglycerides to phospholipids by inhibiting DGAT. This increase in phospholipid synthesis might play a role in the restoration of enterocyte absorption function after intestinal mucosa injury.  (+info)

The isoflavone genistein inhibits internalization of enteric bacteria by cultured Caco-2 and HT-29 enterocytes. (6/3466)

The dietary isoflavone genistein is the focus of much research involving its role as a potential therapeutic agent in a variety of diseases, including cancer and heart disease. However, there is recent evidence that dietary genistein may also have an inhibitory effect on extraintestinal invasion of enteric bacteria. To study the effects of genistein on bacterial adherence and internalization by confluent enterocytes, Caco-2 and HT-29 enterocytes (cultivated for 15-18 d and 21-24 d, respectively) were pretreated for 1 h with 0, 30, 100, or 300 micromol/L genistein, followed by 1-h incubation with pure cultures of Listeria monocytogenes, Salmonella typhimurium, Proteus mirabilis, or Escherichia coli. Pretreatment of Caco-2 and HT-29 enterocytes with genistein inhibited bacterial internalization in a dose-dependent manner (r = 0.60-0.79). Compared to untreated enterocytes, 1-h pretreatment with 300 micromol/L genistein was generally associated with decreased bacterial internalization (P < 0. 05) without a corresponding decrease in bacterial adherence. Using Caco-2 cell cultures, decreased bacterial internalization was associated with increased integrity of enterocyte tight junctions [measured by increased transepithelial electrical resistance (TEER)], with alterations in the distribution of enterocyte perijunctional actin filaments (visualized by fluorescein-labeled phalloidin), and with abrogation of the decreased TEER associated with S. typhimurium and E. coli incubation with the enterocytes (P < 0.01). Thus, genistein was associated with inhibition of enterocyte internalization of enteric bacteria by a mechanism that might be related to the integrity of the enterocyte tight junctions, suggesting that genistein might function as a barrier-sustaining agent, inhibiting extraintestinal invasion of enteric bacteria.  (+info)

Transport of rhodamine 123, a P-glycoprotein substrate, across rat intestine and Caco-2 cell monolayers in the presence of cytochrome P-450 3A-related compounds. (7/3466)

Effects of cytochrome P-450 3A- and P-glycoprotein (P-gp)-related compounds, erythromycin, midazolam, ketoconazole, verapamil, and quinidine, on transport of rhodamine 123 (Rho-123), a P-gp substrate, were studied in rat intestine and in Caco-2 cells. Ileum was mainly used in rat studies because this segment showed greater P-gp-mediated Rho-123 transport. In an in vitro everted rat ileum, all the compounds examined significantly inhibited the transport of Rho-123 from serosal to mucosal surfaces across the intestine, with different inhibitory potencies among these compounds. In an in vivo rat study, the exsorption of Rho-123 from blood to the intestinal lumen, which was evaluated as exsorption clearance of Rho-123 under a steady-state plasma concentration of Rho-123, was also inhibited when these compounds were added to the intestinal lumen. Similarly, transepithelial transport of Rho-123 from the basolateral to apical side across Caco-2 cell monolayers was inhibited by these compounds. A linear relationship was observed in their inhibitory potencies on Rho-123 transport between in vitro and in vivo studies using rat ileum and between studies with rat ileum and Caco-2 cells. P-gp-mediated transport across the intestine was found to be inhibited not only by P-gp-related but also by all the cytochrome P-450 3A-related compounds examined. Within experimental error, the relative inhibitory potencies were the same between the studies with rat ileum (in vivo, in vitro) and those with Caco-2 cells. Thus, it is suggested that the function of P-gp and its sensitivity to these drugs may be similar in rat intestine and Caco-2 cells.  (+info)

Interactions of a nonpeptidic drug, valacyclovir, with the human intestinal peptide transporter (hPEPT1) expressed in a mammalian cell line. (8/3466)

The results of previous work performed in our laboratory using an in situ perfusion technique in rats and rabbit apical brush border membrane vesicles have suggested that the intestinal uptake of valacyclovir (VACV) appears to be mediated by multiple membrane transporters. Using these techniques, it is difficult to characterize the transport kinetics of VACV with each individual transporter in the presence of multiple known or unknown transporters. The purpose of this study was to characterize the interaction of VACV and the human intestinal peptide transporter using Chinese hamster ovary (CHO) cells that overexpress the human intestinal peptide transporter (hPEPT1) gene. VACV uptake was significantly greater in CHO cells transfected with hPEPT1 than in cells transfected with only the vector, pcDNA3. The optimum pH for VACV uptake was determined to occur at pH 7.5. Proton cotransport was not observed in hPEPT1/CHO cells, consistent with previously observed results in tissues and Caco-2 cells. VACV uptake was concentration dependent and saturable with a Michaelis-Menten constant and maximum velocity of 1.64 +/- 0.06 mM and 23.34 +/- 0.36 nmol/mg protein/5 min, respectively. A very similar Km value was obtained in hPEPT1/CHO cells and in rat and rabbit tissues and Caco-2 cells, suggesting that hPEPT1 dominates the intestinal transport properties of VACV in vitro. VACV uptake was markedly inhibited by various dipeptides and beta-lactam antibiotics, and Ki values of 12.8 +/- 2.7 and 9.1 +/- 1.2 mM were obtained for Gly-Sar and cefadroxil at pH 7.5, respectively. The present results demonstrate that VACV is a substrate for the human intestinal peptide transporter in hPEPT1/CHO cells and that although transport is pH dependent, proton cotransport is not apparent. Also, the results demonstrate that the hPEPT1/CHO cell system has use in investigating the transport kinetics of drugs with the human intestinal peptide transporter hPEPT1; however, the extrapolation of these transport properties to the in vivo situation requires further investigation.  (+info)

*Lactobacillus bulgaricus GLB44

... difficile cells in colorectal cells, and thus prevent Antibiotic Associated Diarrhea. Due to more than a century of safe use, ... bulgaricus strains to Caco-2 Cells" There are also numerous studies that outline the antimicrobial activity of L. bulgaricus ... Adherence to mucus and human epithelial cells and cell lines and the ability to reduce pathogen adhesion to surfaces is ... Resistance to gastric acidity Bile acid resistance Adherence to mucus and/or human epithelial cells and cell lines ...

*NFYA

Molecular Cell. 55 (5): 708-22. doi:10.1016/j.molcel.2014.07.005. PMC 4157648 . PMID 25132174. Mantovani R (October 1999). "The ... "Transcription factor NF-Y regulates differentiation of CaCo-2 cells". Archives of Biochemistry and Biophysics. 407 (1): 39-44. ... "The NFY transcription factor inhibits von Willebrand factor promoter activation in non-endothelial cells through recruitment of ... "Histone-fold domain protein NF-Y promotes chromatin accessibility for cell type-specific master transcription factors". ...

*Gluten immunochemistry

These cells cause B-cells that recognize gliadin to proliferate. The B-cells mature into plasma cells producing anti-gliadin ... May 2007). "Intestinal translocation capabilities of wheat allergens using the Caco-2 cell line". J. Agric. Food Chem. 55 (11 ... p38 MAP kinase activation by CD3 negative cells (Lamina proxima mononuclear cells) and CD83 on dendritic cells Maiuri L, Ciacci ... The resulting structure can be presented by APC (with the same gliadin recognizing DQ isoforms) to T-cells, and B-cells can ...

*CLCA1

The encoded protein is expressed as a precursor protein that is processed into two cell-surface-associated subunits, although ... 2002). "pCLCA1 becomes a cAMP-dependent chloride conductance mediator in Caco-2 cells". Biochem. Biophys. Res. Commun. 298 (4 ... 2003). "A calcium-activated chloride channel blocker inhibits goblet cell metaplasia and mucus overproduction". Novartis Found ... 334 (2): 569-76. doi:10.1016/j.bbrc.2005.06.122. PMID 16023076. CLCA1 protein, human at the US National Library of Medicine ...

*Shigella

... helping cell-to-cell spread.[citation needed] After invasion, Shigella cells multiply intracellularly and spread to neighboring ... "Shigella flexneri Enters Human Colonic Caco-2 Epithelial Cells through the Basolateral Pole". Infection and Immunity. 60 (1): ... Hence, Shigella cells may cause dysentery. In rare cases, young children may have seizures. Symptoms can take as long as a week ... Shigella species invade the host through the M-cells interspersed in the gut epithelia of the small intestine, as they do not ...

*Viral disease

"Identification of Host Cell Factors Associated with Astrovirus Replication in Caco-2 Cells". J. Virol. 89 (20): 10359-70. doi: ... "Alphaherpesvirus axon-to-cell spread involves limited virion transmission". Proceedings of the National Academy of Sciences. ... because they do not themselves increase the white blood cell count. Laboratory investigation may be useful in diagnosing ... attach to and enter susceptible cells. Basic structural characteristics, such as genome type, virion shape and replication site ...

*Parallel artificial membrane permeability assay

2005). Caco-2 permeability of weakly basic drugs predicted with the Double-Sink PAMPA pKaflux method. Pharm. Sci., 24: 333-349 ... 2004). PAMPA - a drug absorption in vitro model 7. Comparing rat in situ, Caco-2, and PAMPA permeability of fluoroquinolones. ... Drug development Drug discovery Lipid bilayer Caco-2 cell-based permeability Ottaviani, Giorgio; Martel, Sophie; Carrupt, ... including Caco-2 cultures, the gastrointestinal tract, blood-brain barrier and skin. The donor and/or acceptor compartments may ...

*FABP2

1998). "Differential regulation of intestinal and liver fatty acid-binding proteins in human intestinal cell line (Caco-2): ... Cell Res. 244 (2): 441-7. doi:10.1006/excr.1998.4186. PMID 9806794. Darimont C, Gradoux N, de Pover A (1999). "Epidermal growth ... They may also be responsible in the modulation of cell growth and proliferation. Intestinal fatty acid-binding protein 2 gene ... 2: 7. doi:10.1186/1471-2156-2-7. PMC 31346 . PMID 11299043. Makowski L, Boord JB, Maeda K, et al. (2001). "Lack of macrophage ...

*SLC22A5

2003). "Expression and localization of organic cation/carnitine transporter OCTN2 in Caco-2 cells". Am. J. Physiol. ... 2004). "Downregulation of carnitine acyltransferases and organic cation transporter OCTN2 in mononuclear cells in healthy ... 3.0.CO;2-8. PMID 10612840. Wang Y, Kelly MA, Cowan TM, Longo N (2000). "A missense mutation in the OCTN2 gene associated with ... 3.0.CO;2-3. PMID 10679939. Ohashi R, Tamai I, Inano A, et al. (2002). "Studies on functional sites of organic cation/carnitine ...

*Caprin-1

Mol Cell Biol. 27 (6): 2324-42. doi:10.1128/MCB.02300-06. PMC 1820512 . PMID 17210633. Grill B, Wilson GM, Zhang KX, Wang B, ... which transcytoses bidirectionally in Caco-2 cells". J Biol Chem. 270 (35): 20717-23. doi:10.1074/jbc.270.35.20717. PMID ... Mediate Its Interaction with G3BP-1 and Its Induction of Phosphorylation of Eukaryotic Translation Initiation Factor 2α, Entry ...

*CYP3A4

"Vitamin D Receptor-Mediated Upregulation of CYP3A4 and MDR1 by Quercetin in Caco-2 cells". Planta Medica. 82 (1-2): 121-30. doi ... 15-epoxyeicosatrienoic acids which stimulate these cells to grow, The cytochrome P450 is also reported to have fatty acid ... CYP3A4 promotes the growth of various types of human cancer cell lines in culture by producing (±)-14, ... 218 (2): 585-95. doi:10.1111/j.1432-1033.1993.tb18412.x. PMID 8269949. Inoue K, Inazawa J, Nakagawa H, Shimada T, Yamazaki H, ...

*ENPP7

... alkaline sphingomyelinase in colon cancer HT-29 cells and a differentiation-related expression of the wild-type enzyme in Caco- ... can inhibit cell proliferation and stimulate cell differentiation and apoptosis. Animal studies showed that supplement of SM or ... alkaline sphingomyelinase in colon cancer HT-29 cells and a differentiation-related expression of the wild-type enzyme in Caco- ... Andersson D, Kotarsky K, Wu J, Agace W, Duan RD (July 2009). "Expression of alkaline sphingomyelinase in yeast cells and anti- ...

*Self-microemulsifying drug delivery system

"Effect of self-microemulsifying drug delivery systems containing Labrasol on tight junctions in Caco-2 cells". Eur J Pharm Sci ... "Effect of self-microemulsifying drug delivery systems containing Labrasol on tight junctions in Caco-2 cells.". Eur J Pharm Sci ... SMEDDS in research or development include formulations of the drugs anethole trithione,[2] oridonin,[3][4][5] curcumin,[6] ... 21 (2): 261-70. PMID 15032307. doi:10.1023/B:PHAM.0000016238.44452.f1.. ...

*Tyrosol

As an antioxidant, tyrosol can protect cells against injury due to oxidation. Although it is not as potent as other ... protects against oxidized-LDL-induced injury in Caco-2 cells". J. Nutr. 129 (7): 1269-1277. PMID 10395586. Miró-Casas E, Covas ... Z. Charrouf and D. Guillaume, American Journal of Food Technology, 2007, 2, pages 679-683, doi:10.3923/ajft.2007.679.683 ...

*Peptide transporter 1

Cell Biol. 119 (1): 37-43. doi:10.1007/s00418-002-0479-y (inactive 2017-01-16). PMID 12548404. Neumann J, Brandsch M (2003). " ... Sun BW, Zhao XC, Wang GJ, Li N, Li JS (2003). "Hormonal regulation of dipeptide transporter (PepT1) in Caco-2 cells with normal ... "An oligopeptide transporter is expressed at high levels in the pancreatic carcinoma cell lines AsPc-1 and Capan-2". Cancer Res ... "Thyroid hormone regulates the activity and expression of the peptide transporter PEPT1 in Caco-2 cells". Am. J. Physiol. ...

*Caveolin 1

Cell Res. UNITED STATES. 247 (2): 380-8. doi:10.1006/excr.1998.4379. ISSN 0014-4827. PMID 10066366. Liou, J Y; Deng W G; Gilroy ... The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. ... Frank PG, Lisanti MP (2007). "Caveolin-1 and liver regeneration: role in proliferation and lipogenesis". Cell Cycle. 6 (2): 115 ... Cell Sci. England. 115 (Pt 23): 4457-67. doi:10.1242/jcs.00130. ISSN 0021-9533. PMID 12414992. Scherer, P E; Lewis R Y; Volonte ...

*Glutamate aspartate transporter

Cell. Cardiol. 37 (1): 33-41. doi:10.1016/j.yjmcc.2004.04.008. PMID 15242733. Gendreau S, Voswinkel S, Torres-Salazar D, Lang N ... 2000). "EAAT1 is involved in transport of L-glutamate during differentiation of the Caco-2 cell line". Am. J. Physiol. ... Cell Genet. 69 (3-4): 209-10. doi:10.1159/000133965. PMID 7698014. Shashidharan P, Huntley GW, Meyer T, et al. (1995). "Neuron- ... 2000). "Human T-cell lymphotropic virus type 1-infected T lymphocytes impair catabolism and uptake of glutamate by astrocytes ...

*SLC6A6

1992). "Molecular cloning of the cDNA for an MDCK cell Na(+)- and Cl(−)-dependent taurine transporter that is regulated by ... "Regulation of the human taurine transporter by TNF-alpha and an anti-inflammatory function of taurine in human intestinal Caco- ... Han X, Patters AB, Chesney RW (2002). "Transcriptional repression of taurine transporter gene (TauT) by p53 in renal cells". J ... J. Physiol., Cell Physiol. 292 (1): C332-41. doi:10.1152/ajpcell.00634.2005. PMID 16956961. ...

*Xiaochaihutang

"Transport and metabolism of flavonoids from Chinese herbal remedy Xiaochaihu-tang across human intestinal Caco-2 cell ...

*Mutaflor

Inhibition of cell cycle progression for peripheral T-cells and proapoptotic for mucosal T-cells. Gastroenterology 2003;124, No ... êB dependent mechanism in Caco-2 cells. Gastroenterology 126[4], A-579, 2004. Schultz M et al. Preventive Effects of ... Stimulation of intestinal immune cells by E. coli in gnotobiotic piglets. Adv. Mucosal Immunol. 1995, 463-4. McCann ML et al. ... Characterization of the cellular response of polarized T84 epithelial cells induced by the probiotic E. coli strain Nissle 1917 ...

*Solid lipid nanoparticle

Elucidation of intestinal lymphatic absorption mechanism from solid lipid nanoparticles using Caco-2 cell line as in vitro ... To elucidate the absorption mechanism, from solid lipid nanoparticle, human excised Caco-2 cell monolayer could be alternative ... "Elucidation of intestinal absorption mechanism of carvedilol-loaded solid lipid nanoparticle using Caco-2 cell line as an model ... 199 (2): 167-77. PMID 10802410. Studies on binary lipid matrix based solid lipid nanoparticles of repaglinide: in vitro and in ...

*SLC23A1

Cell Physiology. 283 (6): C1696-704. doi:10.1152/ajpcell.00461.2001. PMID 12388072. Boyer JC, Campbell CE, Sigurdson WJ, Kuo SM ... "Up-regulation and polarized expression of the sodium-ascorbic acid transporter SVCT1 in post-confluent differentiated CaCo-2 ... "Regulation of the human vitamin C transporters expressed in COS-1 cells by protein kinase C [corrected]". American Journal of ... "Molecular characterization of two novel transporters from human and mouse kidney and from LLC-PK1 cells reveals a novel ...

*Mevastatin

CoA reductase inhibitor mevastatin enhances the growth inhibitory effect of butyrate in the colorectal carcinoma cell line Caco ... lovastatin and mevastatin inhibit proliferation and invasion of melanoma cells". BMC Cancer. 8: 9. doi:10.1186/1471-2407-8-9. ... 1S,7S,8S,8aR)-8-{2-[(2R,4R)-4-Hydroxy-6-oxotetrahydro-2H-pyran-2-yl]ethyl}-7-methyl-1,2,3,7,8,8a-hexahydronaphthalen-1-yl (2S)- ... Mevastatin might be considered the first statin drug;[2] clinical trials on mevastatin were performed in the late 1970s in ...

*Coxsackie B4 virus

Coxsackievirus B4 has a cell tropism for natural killer cells and pancreatic islet cells. Infection can lead to beta-cell ... "Study of Coxsackie B viruses interactions with Coxsackie Adenovirus receptor and Decay-Accelerating Factor using Human CaCo-2 ... which can be found on heart muscle cells as well as epithelial and endothelial cells, a conformational change causes the host ... Upon entry of the genome into the cytoplasm of the host cell, the IRES in the 5' UTR recruits ribosomal subunits (cap- ...

*Phenylpyrazole insecticides

... induce cytotoxicity by altering mechanisms involved in cellular energy supply in the human epithelial cell model Caco-2". ... However, they do have the capacity to disrupt epithelial cells in the human intestine and adversely impact human health. ...

*ALPI

2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130-5. ... "Cathepsin C propeptide interacts with intestinal alkaline phosphatase and heat shock cognate protein 70 in human Caco-2 cells ... "Differentiation-dependent activation of the human intestinal alkaline phosphatase promoter by HNF-4 in intestinal cells". Am. J ... 285 (2): G433-41. doi:10.1152/ajpgi.00466.2002. PMID 12660142. Wada A, Wang AP, Isomoto H, et al. (2005). "Placental and ...

*Mineralogy

It is represented by a lattice of points which repeats a basic pattern, called a unit cell, in three dimensions. The lattice ... Calcite is a carbonate mineral (CaCO3) with a rhombohedral crystal structure. ... Commonly pronounced /ˌmɪnəˈrɒlədʒi/[1][2] due to the common phonological process of anticipatory assimilation, especially in ... 2 (6): 388. doi:10.2113/gselements.2.6.388.. *^ Higgins, Michael D.; Smith, Dorian G. W. (October 2010). "A census of mineral ...

*S100G

2004). "Control of differentiation-induced calbindin-D9k gene expression in Caco-2 cells by cdx-2 and HNF-1α". Am. J. Physiol. ... 185 (2): 663-9. doi:10.1016/0006-291X(92)91676-H. PMID 1610358. Jeung EB, Krisinger J, Dann JL, Leung PC (Sep 1992). "Molecular ... 307 (2): 224-8. doi:10.1016/0014-5793(92)80772-9. PMID 1379540. "Entrez Gene: S100G S100 calcium binding protein G". Balmain N ...
Sodium caprate (CIO), a medium chain fatty acid, is used clinically to enhance rectal absorption of the low molecular weight (MW) drug ampicillin. The main aim of this study was to investigate whether CIO also enhances the permeability of high MW model drugs in a model of the intestinal epithelium. The second aim was to present visual evidence of the route of enhanced transport across the epithelial cell layer. The studies were performed in Caco-2 monolayers cultured on permeable supports. The effects of non-toxic concentrations (≤ 13 mM) of CIO on drug transport across the monolayers was studied using monodisperse 14C-polyethylene glycols (MW 238-502; 14C-PEGs), 125I-Arg8-vasopressin (MW 1,208), 125I-insulin (MW 6,000) and FITC-labelled dextrans (MW 4,400 and 19,600; FD4 and FD20 respectively) as model drugs. Electron and confocal laser scanning microscopy were used to demonstrate transport routes across the epithelium. 10 mM C10 increased the permeability of all 14C-PEGs to approximately the ...
Note: DIPLOMES; GR - 53 332; Cornell University, Ithaca USA; . Consultable sur demande à la Bibliothèque de lEPFL / Offered in consultation at the EPFL library.. ...
In order to evaluate the release of zonulin during the time of observation, the area under the curve (AUC) was calculated. All data are expressed as mean and SEM. Differences were considered significant at P < 0.05. A specific software package (SigmaStat for Windows version 3.00 SPSS Inc. San Jose, CA, USA) was used. Results Effects of gliadin and L.GG. treatments on Caco-2 monolayer barrier function (TER and lactulose flux) TER measurements were determined after the addition of viable L.GG, L.GG-HK and L.GG-CM to polarized monolayers of Caco-2 cells seeded on Transwell filter inserts. TER was measured before the addition of bacteria, at time zero (immediately after the bacteria administration) and then at various time intervals ranging from. 30 min to 6 h. A slight and not significant Selleck CHIR 99021 increase in TER was observed after 90 min from the addition of viable L.GG, as well as L.GG-HK and L.GG-CM to Caco-2 cells compared to control cells (data not shown). Addition of gliadin to the ...
hi friends ! iam handling few cell lines ! we recently bought Caco-2 cell line. i have ended up mixing caco-2 cells with hybridoma cells and now iam unable to remove this contamination. Hybridoma cell line has a doubling time of just 24hrs is growing fast and soon will replace Caco-2 cells. caco-2 cells are really slow growing cells. i tried few mwthods to isolate them but failed ...
Dietary non-heme iron contains ferrous [Fe(II)] and ferric [Fe(III)] iron fractions and the latter should hydrolyze, forming Fe(III) oxo-hydroxide particles, on passing from the acidic stomach to less acidic duodenum. Using conditions to mimic the in vivo hydrolytic environment we confirmed the formation of nanodisperse fine ferrihydrite-like particles. Synthetic analogues of these (~ 10 nm hydrodynamic diameter) were readily adherent to the cell membrane of differentiated Caco-2 cells and internalization was visualized using transmission electron microscopy. Moreover, Caco-2 exposure to these nanoparticles led to ferritin formation (i.e., iron utilization) by the cells, which, unlike for soluble forms of iron, was reduced (p=0.02) by inhibition of clathrin-mediated endocytosis. Simulated lysosomal digestion indicated that the nanoparticles are readily dissolved under mildly acidic conditions with the lysosomal ligand, citrate. This was confirmed in cell culture as monensin inhibited Caco-2 utilization
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
La linea cellulare di adenocarcinoma del colon Caco-2, se coltivata a confluenza, differenzia spontaneamente verso un fenotipo intestinale. Poco è noto riguardo ai meccanismi che regolano il processo differenziativo di queste cellule che mostrano mutazioni inattivanti a livello delle proteine APC e β-catenina. Queste mutazioni sono solitamente associate ad una stimolazione costitutiva di geni regolativi del ciclo cellulare quali Myc e la ciclina D1. Nonostante ciò, le Caco-2 sono in grado di uscire dal ciclo cellulare e mostrano un controllo trascrizionale e traduzionale, regolato nel tempo, di geni specifici del differenziamento intestinale. Attraverso lo studio dellespressione e modulazione di RNA e di proteine nel processo differenziativo abbiamo notato che le proteine Myc e ciclina D1, entrambe bersaglio trascrizionale del complesso β-catenina/TCF, sono regolate in maniera differente. Abbiamo quindi deciso di indagare il ruolo della regolazione di Myc nel differenziamento delle Caco-2. ...
Consistent with recent observations (22), we found that Caco-2 cells did not express HGF but its receptor alone. The highest levels of c-Met (mRNA and protein) occurred 8-15 days after plating. HGF is known to stimulate the proliferation of most epithelial cells in vitro. In this study, we found that 10 ng/ml HGF exerted only a moderate mitogenic effect on Caco-2 cells, consistent with the results of other studies in which Caco-2 cells were incubated with the same concentration of HGF (22). This led to a modest shortening of the time required to reach confluence. The differentiation process therefore began earlier in the presence of HGF, and this may partly account for the large increase in expression of E-cadherin and villin proteins noted on immunoblots 4 days after plating. However, at that time, the magnitude of increase observed in the amounts of these two proteins in cells incubated with HGF compared with controls was much greater than that observed between cells before and after ...
The dynamic interactions of metabolism and secretion, and the role of transporters on drug absorption have been under considerable debate. The thorough understanding of intestinal processes would be learnt through modeling of intestinal data only in the absence of the contribution from the liver. Recent studies on the interactions between the P450s and Pgp in vitro have led to the conclusion that intestinal metabolism is enhanced by the secretory action of P-glycoprotein due to an increase in the mean residence time (MRT) of drug in the intestine (Benet and Cummins, 2001; Cummins et al., 2001; Johnson et al., 2001). Theoretical examinations on Caco-2 cells and intestinal vascular perfusion systems support the notion that the mean residence time was increased, but metabolism under linear conditions was in fact decreased in the presence of secretion, even though the mean residence time has increased; under nonlinear metabolism, instances exist whereby the rate of metabolite accrual may increase, ...
antibody-antibodies.com is the marketplace for research antibodies. Find the right antibody for your research needs. Adherence potential of indigenous lactic acid bacterial isolates obtained from fermented foods of Western Himalayas to intestinal epithelial Caco-2 and HT-29 cell lines.
Caco-2 monolayers grown on permeable filters for 21 days in bicameral Transwell chambers were incubated with control medium, medium containing cytomix (CM), a cocktail containing the pro-inflammatory cytokines, IL-1β (1 μg/ml), TNF (10 ng/ml) and IFN-γ (1000 U/ml), or medium containing CM and one of these other pharmacologic agents: ethyl pyruvate (EP; H2O2 scavenger; 10 mM): PJ34 (PARP inhibitor; 5 μM): 3-aminobenzamide (3-AB; PARP inhibition; 3 μM): FeTPPS (ONOO- decomposition catalyst; 50 μM): C-PTIO (NO• scavenger; 100 μM): PDTC (NF-κB inhibitor; 100 μM) or L-NIL (selective iNOS inhibitor; 20 μM). Permeability was expressed as the apical-to-basolateral clearance (nLcm-1 h-1) of fluorescein-labelled dextran (FD4) during the last 48 hours of incubation. Expression of occludin and ZO-1 were assessed by Western blot and immunohistochemistry. ...
The C2BBe1 (brush border expressing) cell line was cloned in 1988 from the Caco-2 cell line (ATCC® HTB-37™) by limiting dilution. The clone was selected on the basis of morphological homogeneity and exclusive apical villin localization.
ICK - ICK (untagged)-Kinase deficient mutant (K33M) of Human intestinal cell (MAK-like) kinase (ICK), transcript variant 2 available for purchase from OriGene - Your Gene Company.
Wampler, Jennifer L. et al "Heat Shock Protein 60 Acts as a Receptor for the Listeria Adhesion Protein in Caco-2 Cells." Infection and Immunity 72.2 (2004): 931-936. Web. 06 Dec. 2019. ...
Publications, Species, Genomes and Genes, Scientific Experts, Research Topics about Biliary and Intestinal Cell Models of Drug Toxicity
Mods - I used search but the answer was not readily apparent in the fifty threads that came back I just have a couple quick questions so shove that...
Background & Aims: For optimal nutrient absorption to occur, the enterocyte must express a range of specialist ion-driven carrier proteins that function cooperatively in a linked and mutually dependent fashion. Thus, absorption via the human intestinal H+-coupled di/tripeptide transporter (hPepT1) is dependent on maintenance of the trans-apical driving force (the H+-electrochemical gradient) established, in part, by brush-border Na+/H+ exchanger (NHE3) activity. This study aimed to examine whether physiologic regulation of NHE3 activity can limit hPepT1 capacity and, therefore, protein absorption after a meal. Methods: hPepT1 and NHE3 activities were determined in intact human intestinal epithelial Caco-2 cell monolayers by measurements of [14C]glycylsarcosine transport and uptake, 22Na+-influx, H+-influx, and H+-efflux. Expression of NHE regulatory factors was determined by reverse-transcriptase polymerase chain reaction. Results: Optimal dipeptide transport was observed in the presence of a ...
Summary The pathogenesis of campylobacter enteritis is not well understood, but invasion into and translocation across intestinal epithelial cells may be involved in the disease process, as demonstrated for a number of other enteric pathogens. However, the mechanisms involved in these processes are not clearly defined for campylobacters. In this study, isolates were compared quantitatively in established assays with the enterocyte-like cell line, Caco-2, to determine the extent to which intracellular invasion contributes to translocation across epithelial cell monolayers, and whether isolates vary in this respect. Ten fresh Campylobacter isolates were compared and shown to differ in invasiveness by a factor of 10-fold by following their recovery from gentamicin-treated Caco-2 cells grown on non-permeable tissue-culture wells. Four of these isolates with contrasting invasive ability were also shown to vary in their ability to translocate across Caco-2 cells grown on semi-permeable Transwell inserts by a
Bovine colostrum is well known for its beneficial properties on health and development. It contains a wide variety of bioactive ingredients that are known to promote a number of cellular processes. Therefore the use of colostrum whey as a feed additive to promote intestinal health has been proposed, yet little is known about mechanisms implicated in its beneficial properties on intestinal epithelial cells. In the present paper, casein were removed from bovine colostrum and the remaining liquid, rich in bioactive compounds, was evaluated for its capacity to modulate cellular processes in porcine intestinal epithelial cell line IPEC-J2 and human colon adenocarcinoma cell line Caco-2/15. First, we verified the effect of colostrum whey and cheese whey on processes involved in intestinal wound healing, including cell proliferation, attachment, morphology and migration. Our results showed that colostrum whey promoted proliferation and migration, and decreased specifically the attachment of Caco-2/15 ...
The benzenearsonate, Roxarsone, has been used since 1944 as an antimicrobial, growth-promoting poultry feed additive. USGS and EPA report that Roxarsone (4-hydroxy-3-nitrobenzenearsonate) and metabolites, including AHBA (3-amino-4-hydroxybenzenearsonate), contaminate waterways at greater than 1100 tons annually. To assess human impact of these organic arsenic water contaminants, it was important to study their potential absorption. The human adenocarcinoma cell line, Caco-2, is a model for intestinal absorption. We found proliferative effects on Caco-2 cells at micromolar levels of these compounds, as monitored by [3H]-thymidine incorporation into DNA. Flow cytometry cell cycle analysis confirmed accumulation in S phase from 21% (control) to 36% (24 hour exposure to 10 μM AHBA). Confluent Caco-2 cells grown on collagen-coated Transwell plates were dosed on the apical side. After exposure, media from apical and basolateral sides were collected separately. Following removal of FBS by 30K centrifugal
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
In this study, using cAMP analogues, as well as the adenylate cyclase activator forskolin and the phosphodiesterase inhibitor, 3-isobutyl-1-methylxanthine, we have shown that cAMP potentiates cytokine induced iNOS activity, protein, and mRNA in the human intestinal epithelial cell line, DLD-1. Cyclic AMP has been shown to induce iNOS expression in many cell types but the majority of these are rat cell lines. Indeed, cAMP responsive element (CRE) sites have been identified in the rat iNOS gene39 and could be involved in the iNOS expression caused by cAMP alone or in combination with cytokines. Nevertheless, cAMP can also decrease iNOS expression in cells of rat origin.28 32 40 To our knowledge, only two studies have reported cAMP enhancement of iNOS expression in human cells, these being monocytes and T cells.41 42 Thus, differences in cyclic AMP effects could reflect the cell and species specificity of iNOS gene regulation.. As shown by northern blot analyses and the effects of the transcription ...
Glutamine supplementation has been advocated for patients requiring parenteral nutritional support. However, the direct effect of glutamine on neoplastic cells is poorly understood. We therefore investigated the effects of glutamine on the proliferation, differentiation, and cell-matrix interactions of two human colon carcinoma cell lines (Caco-2 and SW620) adapted to glutamine-free media. Doubling times were calculated by logarithmic transformation of serial cell counts. Alkaline phosphatase, cathepsin C (dipeptidyl peptidase), lactase, and isomaltase expression (markers of differentiation) were assayed by digestion of synthetic substrates. Adhesion to matrix proteins was assessed by colorimetric quantitation of toluidine blue staining of adherent cells. Surface expression of Caco-2 receptors for matrix proteins (integrins) was studied by biotinylation and immunoprecipitation with specific antibodies. Glutamine (1-10 mm) dose-dependently stimulated Caco-2 proliferation on all matrices studied ...
Author(s): Hirst BH; Simmons NL; Thwaites DT; McEwan GTA. Publication type: Article. Publication status: Published. Journal: Pfluegers Archiv: European Journal of Physiology. Year: 1993. Volume: 425. Issue: 1-2. Pages: 178-180. Print publication date: 01/10/1993. ISSN (print): 0031-6768. ISSN (electronic): 1432-2013. Publisher: Springer. URL: http://dx.doi.org/10.1007/BF00374520. DOI: 10.1007/BF00374520. PubMed id: 8272376. ...
Eicosanoids are lipid mediators synthesized by the arachidonic acid (AA) cascade from membrane polyunsaturated fatty acids (PUFA) trough three distinct pathways: cyclooxygenase, lipooxygenase (LOX) and cytochrome P450 pathways. Eicosapentaenoic acid, an n-3 PUFA, is also substrate of the enzymes of the AA cascade. Thus, 5-LOX gives rise to the 5-series leukotrienes, and 12-LOX generates 12-HEPE, the homologue of 12-HETE when the source is AA. Fish oil supplementation has been used as a preventive measure against a number of diseases including coronary heart disease, cancer, and inflammatory bowel disease (IBD). IBD and other intestinal diseases are associated with the disruption of epithelial barrier function( 1 ). In this sense, we recently demonstrated that PGE2, which is increased in the mucosa of IBD patients, induces the disruption of this barrier in differentiated Caco-2 cells( 2 ). In this line, the present study aims to investigate the potential of 12-LOX derived eicosanoids 12-HETE and ...
Caco-2 assays measure the ability of a drug to be absorbed from the gastrointestinal tract and thereby to evaluate whether the drug can be suitably dosed via an oral route. It also provides information regarding the ability of a compound to serve as a substrate for the P-glycoprotein (Pgp) efflux drug pump.. Caco-2 cells are intestinal epithelial tumor cells that form a polarized monolayer, a well-defined brush border on the apical surface and intercellular junctions when plated on a semipermeable membrane in a Transwell® dish.. ...
BioAssay record AID 695938 submitted by ChEMBL: Apparent permeability across human Caco2 cells st 200 uM measured over 150 mins by LC/MS analysis.
in BMC Cell Biology (2006), 7. Background: The absorptive and goblet cells are the main cellular types encountered in the intestine epithelium. The cell lineage Caco-2 is a model commonly used to reproduce the features of the bowel ... [more ▼]. Background: The absorptive and goblet cells are the main cellular types encountered in the intestine epithelium. The cell lineage Caco-2 is a model commonly used to reproduce the features of the bowel epithelium. However, there is a strong debate regarding the value of Caco-2 cell culture to mimick in vivo situation. Indeed, some authors report in Caco-2 a low paracellular permeability and an ease of access of highly diffusible small molecules to the microvilli, due to an almost complete lack of mucus. The HT29-5M21 intestinal cell lineage is a mucin-secreting cellular population. A co-culture system carried out in a serum-free medium and comprising both Caco-2 and HT29-5M21 cells was developed. The systematic use of a co-culture system requires the ...
TY - JOUR. T1 - Permeability and toxicity characteristics of L-cysteine and 2-methyl-thiazolidine-4-carboxylic acid in Caco-2 cells. AU - Kartal-Hodzic, Alma. AU - Marvola, Tuuli. AU - Schmitt, Mechthild. AU - Harju, Kirsi. AU - Peltoniemi, Marikki. AU - Siven, Mia PY - 2013/11. Y1 - 2013/11. KW - 317 Pharmacy. U2 - 10.3109/10837450.2012.659253. DO - 10.3109/10837450.2012.659253. M3 - Article. VL - 18. SP - 1288. EP - 1293. JO - Pharmaceutical Development and Technology. JF - Pharmaceutical Development and Technology. SN - 1083-7450. IS - 6. ER - ...
Cell proliferation, differentiation, and death are a continuous process in intestinal mucosa homeostasis. Although cancer cell lines, such as Caco-2 and HT29, may not completely replicate normal growth characteristics, they provide useful in vitro models for study of cellular growth control. The GSH/GSSG redox (Eh) has been strongly implicated to be involved in all three stages of cell development. When induced chemically to differentiate, HT29 cells became more oxidized by 60 mV compared with the proliferative cell population (22). In addition, differentiation induced by butyrate in Caco-2 cells was accompanied by apoptosis (23), which results in a further oxidation of GSH/GSSG redox (6, 17). Present data confirm and extend these results, showing that 1) oxidation of the GSH pool occurs as Caco-2 cells spontaneously progress from proliferation to differentiation, 2) oxidation of the extracellular Cys/CySS pool occurs under the same conditions, 3) increased apoptosis (albeit at low levels) is ...
Study of the human intestines is greatly improved with the addition of an anatomical model. Anatomy Warehouse offers the best in anatomical education, plus free shipping on many orders.
Judy D. Whittimore is the author of this article in the Journal of Visualized Experiments: Using Caco-2 Cells to Study Lipid Transport by the Intestine
Balance of forces considered in MSM.(a) Cell monolayer is considered as a thin sheet of cells. Each cell in the monolayer exerts traction, , on the substrate. A
TY - JOUR. T1 - FTF and LRH-1, two related but different transcription factors in human Caco-2 cells. T2 - Their different roles in the regulation of bile acid transport. AU - Pan, Debra H.. AU - Chen, Frank. AU - Neimark, Ezequiel. AU - Li, Xiaoping. AU - Shneider, Benjamin L.. PY - 2005/12/30. Y1 - 2005/12/30. N2 - The apical sodium dependent bile acid transporter (ASBT) mediates ileal bile acid reabsorption. The transcription factors, liver receptor homologue-1 (LRH-1:mouse) and fetoprotein transcription factor (FTF:human), are presumably orthologues. Bile-acid induced negative feedback regulation of mouse (m) and human (h) ASBT occurs via LRH-1 and RAR/RXR, respectively. hASBT has a potential FTF cis-element, although its functional role is unknown. hASBT and mASBT promoter constructs and an FTF cis-element mutated hASBT (hASBT/FTFμ) were assessed in human Caco-2 cells treated with chenodeoxycholic acid (CDCA) and/or co-transfected with hFTF, mLRH-1, or specific small interfering FTF or ...
Contluent cultures of Caco-2 cells, a human colonic carcinoma cell line, undergo differentiation into enterocyte-like cells and are widely used as a model system to study the expression of membrane peptidases on the apical brush border [I-41 Indirect irnniunofluorescent staining has previously shown dipeptidylpeptidase IV (DPPIV, EC 3 4 14 5) to be homogeneously distributed on the surface of Caco-2 cells [ I ] whereas endopeptidase-24 1 1 (E-24 I I , EC 3 4 24 I I ) was found on the surface of some cells but not on others [4] This observation has also been reported by Jalal e/ d. [ S ] who have shown that E-24 1 1 is expressed by all cells but in some is confined to an intracellular pool In the present study the cellsurface and intracellular expression of five membrane peptidases on confluent cultures of Caco-2 cells has been investigated using indirect immunofluorescent staining The membrane peptidases studied were DPPIV, E-24 I I , angiotensin converting enzyme (ACE, EC 3 4 15 I). ...
Flavonoids are a broad collection of polyphenolic compounds ubiquitously found in foods of plant origin. Previous studies suggested that they are beneficial in protecting against many chronic diseases, such as cancers, cardiovascular diseases and neurological disorders. There is also evidence that flavonoids have beneficial effects on modifying glucose absorption and are thus regarded as potential anti-diabetic agents. To address this we investigated the effects of a flavonoid-rich berry extract on glucose transport in intestinal epithelial Caco-2 cells and C2C12 muscle cells. In Caco-2 cells, acute exposure to berry extract inhibited glucose uptake. Prolonged incubation down-regulated the gene expression of SLUT2 and SGLT1, with a concomitant up-regulation of let-7a miRNA. Using bioinformatic tools, let-7a is predicted to target the mRNA of GLUT2, potentially down-regulating its expression. In C2C12 myotubes, chronic incubation of berry extract did not have any effect on basal or ...
A water-soluble beta-D-glucan was obtained from fruiting bodies of Piptoporus betulinus, by hot aqueous extraction followed by freeze-thawing procedure and dialysis. Its molar mass distribution and conformational behavior in solution was assessed by size-exclusion chromatography coupled with multiangle laser light scattering, showing a polysaccharide with an average molecular weight of 2.5 x 10(5) Da with a random coil conformation for molecular weights below 1 x 10(6) Da. Typical signals of beta-(1 -, 3)-linkages were observed in NMR spectrum (delta 102.7/4.76; 102.8/4.74; 102.9/4.52; and delta 85.1/3.78; 85.0/3.77) and also signals of O-6 substitution at delta 69.2/4.22 and 69.2/3.87. The analysis of partially O-methylated alditol acetates corroborates the NMR results, indicating the presence of a beta-D-glucan with a main chain (1 -, 3)-linked, substituted at O-6 by single-units of glucose. The beta-D-glucan showed no toxicity on human colon carcinoma cell line (Caco-2) up to 1000 mu g mL(-1) ...
Sigma-Aldrich offers abstracts and full-text articles by [Zheng Ruan, Shiqiang Liu, Yan Zhou, Shumei Mi, Gang Liu, Xin Wu, Kang Yao, Houssein Assaad, Zeyuan Deng, Yongqing Hou, Guoyao Wu, Yulong Yin].
Millions of micro-organisms populate our orifices from the moment we are born until we die. The microbiome represents the totality of micro-organisms that coexist with us rather peacefully and which we acquire purposefully from our environment. Health is associated with a balance between commensal and pathogenic microbes, which control the intestinal barrier integrity through the production of mucus and lipid metabolites, such as short-chain fatty acids.
Two peptides from soybean β-conglycinin, i.e., YVVNPDNDEN (peptide 2) and YVVNPDNNEN (peptide 3), are known to be absorbed by human enterocytes. The former is ...
Cytotoxicity assay in 2D and 3D culture Different assays has been performed by HCS Pharma on different cell lines as HT29, Hela, HepG2, Caco-2 ... Here are few examples. If you want to know more about these assays, dont hesitate to contact us. To learn more on our model, see also our poster presented in different
Copper, Ability, Adenocarcinoma, Apoptosis, Caco-2 Cells, Cell, Cell Death, Cells, Colon, Crystallography, Culture, Death, DNA, DNA Cleavage, DNA Damage, Efficiency, Environment, Fluorescence, Gene, Human
肠上皮细胞(intestinal epithelial cells,IECs)是机体抵御病原微生物的第一道防线,是联系宿主和肠道微生物的主要桥梁.IECs与微生物的相互作用可能会引起免疫失调,然而益生菌能通过屏障与免疫调节功能来预防肠道疾病的发生.首先,益生菌具有生物拮抗功能,可以通过争夺营养,代谢产物,以及占位效应来调节肠道内微生物菌群,抑制肠道外源性潜在致病菌的生长;其次,益生菌能够改善机体的免疫功能,IECs通过模式识别受体识别益生菌及代谢产物,调节多个信号通路,调控受体的表达,激发机体自身非特异性免疫应答,从而产生一系列的免疫保护反应.另外,益生菌能够诱导肠道上皮细胞产生大量富含黏蛋白 ...
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BioAssay record AID 86084 submitted by ChEMBL: In vitro cytotoxicity against human colon adenocarcinoma cell line HT-29 at 5.9*10e-5M.
Citation: Zeng, H., Jackson, M.I., Cheng, W., Combs, G.F. 2010. Chemical form of selenium affects its uptake, transport and glutathione peroxidase activity in the human intestinal Caco-2 cell model. Biological Trace Element Research. 143:1209-1218. Interpretive Summary: Determining the effect of selenium (Se) chemical form on uptake and transport in human intestinal cells is critical to assess Se bioavailability. In the present study, we measured the uptake and transport of various Se compounds in the human intestinal Caco-2 cell model. We found that two sources of selenomethionine, the purified L-isomer (SeMet) and an in vitro-digested, Se-enriched yeast each increased intracellular Se content more effectively than selenite or methylselenocysteine (SeMSC). Interestingly, SeMSC, SeMet and digested Se-enriched yeast were transported at comparable rates from the apical to basolateral sides, each being about 3 fold that of selenite. This observation suggests the presence of a transcellular pathway ...
Vibrio parahaemolyticus is a food borne gastro-intestinal pathogen of major global importance. The organism induces severe inflammatory diarrhea in individuals who become infected due to the consumption of contaminated seafood. V. parahaemolyticus is known to exert pathogenic effects upon host cells by the translocation of effector proteins into the host cell, using a type three secretion injectisome, however little is known about the molecular interactions which allow colonisation and persistence of the organism in the intestinal epithelium. This study analysed the adherence and invasion of V. parahaemolyticus using the established intestinal cell line Caco-2. V. parahaemolyticus was found to adhere at high levels to Caco-2 cells. A small number of the adherent bacteria became internalised and were subsequently capable of intracellular proliferation. A genomic library based approach was undertaken in order to identify novel proteins conferring adhesive and invasive traits (adhesins/invasins) ...
After ingestion via contaminated food or water, enterohaemorrhagic E. coli colonises the intestinal mucosa and produces Shiga toxins (Stx). No Stx-specific secretion system has been described so far, and it is assumed that Stx are released into the gut lumen after bacterial lysis. Human intestinal epithelium does not express the Stx receptor Gb3 or other Stx binding sites, and it remains unknown how Stx cross the intestinal epithelial barrier and gain access to the systemic circulation. This review summarises current knowledge about the influence of the intestinal environment on Stx production and release, Stx interaction with intestinal epithelial cells and intracellular uptake, and toxin translocation into underlying tissues. Furthermore, it highlights gaps in understanding that need to be addressed by future research.
Tick-borne encephalitis virus (TBEV) is one of the most important vector-borne viruses in Europe and Asia. The transmission mainly occurs by the bite of an infected tick. Consuming of rough milk products from infected livestock animals also occasionally cause TBE cases. Human intestinal Caco-2 cells were used to investigate the pathogenesis caused by TBEV. During TBEV infection Caco-2 monolayers showed morphological changes with significant vacuolization. Ultrastructural analysis revealed dilatation of the rough endoplasmic reticulum and further enlargement to TBEV containing caverns. Caco-2 monolayers showed an intact epithelial barrier with stable transepithelial electrical resistance (TER). Concomitantly, viruses were detected in the basolateral medium, taken up via a transcytosis pathway. TBEV cell entry was efficiently blocked with different inhibitors, suggesting that actin filaments and microtubules are important for PI3K-dependent endocytosis. Moreover, experimental fluid uptake assay ...
Epithelial damage caused by intestinal permeation enhancers is a source of debate over their safety. The medium chain fatty acid, sodium caprate (C10), causes reversible membrane perturbation at high dose levels required for efficacy in vivo, so the aim was to model it in vitro. Exposure of Caco-2 monolayers to 8.5mM C10 for 60min followed by incubation in fresh buffer led to (i) recovery in epithelial permeability (i.e. transepithelial electrical resistance (TEER) and apparent permeability coefficient (Papp) of [(14)C]-mannitol), (ii) recovery of cell viability parameters (monolayer morphology, plasma membrane potential, mitochondrial membrane potential, and intracellular calcium) and (iii) reduction in mRNA expression associated with inflammation (IL-8). Pre-incubation of monolayers with a mucosal prostaglandin cytoprotectant was attempted in order to further decipher the mechanism of C10. Misoprostol (100nM), inhibited C10-induced changes in monolayer parameters, an effect that was partially ...
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We studied arachidonic acid metabolism and the expression of cyclooxygenase (Cox) and 15-lipoxygenase (15-LO) in the human colorectal carcinoma cell line, Caco-2, which undergo apoptosis and cell differentiation in the presence of sodium butyrate (NaBT). Caco-2 cells expressed very low levels of Cox-1 but highly expressed Cox-2. NaBT treatment shifted the arachidonic acid metabolites by cell lysates from prostaglandins to 15-hydroxyeicosatetraenoic acid, indicating the presence of a 15-LO. Linoleic acid, an excellent substrate for 15-LO, was metabolized poorly by the Caco-2 cells, but NaBT treatment shifted metabolism to 15-LO metabolite, 13(S)-hydroxyoctadecadienoic acid. Caco-2 cells expressed a 15-LO but only after treatment with NaBT, as determined by Northern blotting. Immunoblotting with anti-human 15-LO antibody detected a 72-kDa band in NaBT-treated Caco-2 cells. Expression of 15-LO mRNA was dependent on the duration of NaBT treatment, with the highest expression observed between 10 and ...
Increased mucosal infiltration by leucocytes is a characteristic feature of inflammatory bowel disease and chemotactic factors play a key role in the recruitment of inflammatory cells. Intestinal epithelial cells act as the interface between the contents of the gut lumen and the internal milieu, which places them in an ideal position to detect and respond to proinflammatory stimuli.. In this study we have shown that human colonic cell lines, HT29-19A and Caco-2, express distinct patterns of chemokine secretion, both constitutively and after stimulation by inflammatory agonists. HT29-19A cells, which have a phenotype similar to that expected of Cl− secretory cells in the crypt epithelium,18 secreted high levels of both IL-8 and GROα, primarily in response to TNF-α and IL-1. These chemokines were secreted in parallel, probably via a common pathway as judged by their virtually identical time course for secretion and dose responsiveness to cytokine stimulation, suggesting that they may have ...
TY - JOUR. T1 - Phosphorylation of pp62 and pp54 src-like proteins in a rat intestinal cell line in response to gastrin. AU - Singh, Pomila. AU - Narayan, S.. AU - Adiga, R. B.. PY - 1994. Y1 - 1994. UR - http://www.scopus.com/inward/record.url?scp=0028121604&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0028121604&partnerID=8YFLogxK. M3 - Article. C2 - 8074224. AN - SCOPUS:0028121604. VL - 267. JO - American Journal of Physiology - Endocrinology and Metabolism. JF - American Journal of Physiology - Endocrinology and Metabolism. SN - 0193-1849. IS - 2 part 1. ER - ...
The gastrointestinal tract is a principal route of entry and site of persistence of human immunodeficiency virus type 1 (HIV-1). The intestinal mucosa, being rich of cells that are the main target of the virus, represents a primary site of viral replication and CD4+ T-cell depletion. Here, we show both in vitro and ex vivo that HIV-1 of R5 but not X4 phenotype is capable of selectively triggering dendritic cells (DCs) to migrate within 30 min between intestinal epithelial cells to sample virions and transfer infection to target cells. The engagement of the chemokine receptor 5 on DCs and the viral envelope, regardless of the genetic subtype, drive DC migration. Viruses penetrating through transient opening of the tight junctions likely create a paracellular gradient to attract DCs. The formation of junctions with epithelial cells may initiate a haptotactic process of DCs and at the same time favour cell-to-cell viral transmission. Our findings indicate that HIV-1 translocation across the ...
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Results: CTx and C. difficile toxin induced LDH release from E12 monolayers. CTx also elevated intracellular epithelial cAMP, while S. typhimurium induced basolateral IL-8 secretion. Pre-treatment of E12 monolayers with poly(DMAEMA) reduced these effects, but only in the presence of mucus. The polymer co-localised with S. typhimurium in mucus and reduced bacteria-epithelia association. Poly(DMAEMA) was directly bactericidal against S. typhimurium at 1 mg/ml within 30 min ...
Proteoglicanos são macromoléculas complexas compostas de polissacarídeos lineares, os glicosaminoglicanos (GAGs), ligados covalentemente a um core protéico. Os GAGs contêm grupamentos sulfato em várias posições, formando domínios específicos, que permitem a interação com moléculas da matriz extracelular, incluindo os fatores de crescimento. Neste estudo, utilizamos o sistema de co-cultura em Transwell para analisar a interação entre fibroblastos e células de adenocarcinoma colorretal humano, Caco-2, e investigar os efeitos dessa interação na proliferação celular e na síntese de GAGs. Componentes solúveis trocados pelas células nesse sistema, induziram um aumento marcante na proliferação da Caco-2, efeito não observado nos fibroblastos. Um aumento na biossíntese de GAGs por incorporação de 35S-NaSO4, foi observado em ambas linhagens, contudo o aumento mais significativo de condroitim sulfato (CS) foi detectado nas células estromais. A microscopia confocal mostrou um ...
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). The addition of Bovine Insulin has been reported to be useful where there has been slow growth of cells. Split sub-confluent cultures 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37C. NB: During routine subculture the cells should always be subcultured before they achieve confluence ...
Isolation of intestinal epithelial cells - posted in Immunology: Hi, I was wondering whether anyone could share a detailed protocol on how to isolate viable,intact colonic epithelial cells from mice. I have tried numerous methods with EDTA where in the colon is cut longitudinally and then incubated in 5-30mM EDTA shaking at 37 degrees for 15 minute shakes. Ive also tried to evert the colon onto a glass rod and then shake in EDTA. The fractions that come off do not seem to yield any IE...
Screening of P-Glycoprotein Inducers and Activators as Effective Antidotes Against its Toxic Substrates in Caco-2 Cells. The Example of ...
Permeability, Absorption, Cell, Rat, Metoprolol, Caco-2 Cell, Concentration, Drugs, Perfusion, Prodrugs, Human, Classification, Intestine, Jejunum, Membrane, Cancer, Hydrolysis, P-gp, Valine, Bioavailability
Compatible with most robotics and fluid handlers, our automation friendly HTS systems increase growth area and yield. Each is packaged complete with reservoir and receiver plates, so theres no need to purchase parts separately. Customized products are available to meet special requirements.. Corning also offers high throughput screening (HTS) insert plates. These are arrays of individual cell culture inserts connected by a rigid, robotics-friendly holder. This single-unit design makes insert plates ideal for running automated, high throughput drug transport (Caco-2 cells) cell toxicity studies or cell migration and invasion studies ...
EMMPRIN down-regulation by scFv-M6-1B9 intrabody affecting decline of CEA level. Caco-2 cells transduced for 7 days and 10 days were collected as the supernatan
Enhanced oral bioavailability of poorly aqueous soluble drugs encapsulated in a number of lipid-based formulations, including emulsions, micellar systems, self-emulsifying drug delivery systems, liposomes and solid lipid nanoparticles (SLN) via lymphatic delivery has been documented (1). In the present work, SLN were designed for the oral delivery of heparin in order to take advantage from the lymphatic intestinal transport pathway. In order to improve the incorporation of a high hydrophilic compound in a lipid matrix, heparin was "insolubilized" by the coupling with chitosan. In this aim we have developed chitosan/heparin Polyelectrolyte complexes (PEC). Such as systems are able to complex stably heparin (up to pH , 6.8) (2) and after pelletization by centrifugation were embedded in SLN obtaining a hybrid system lipid/chitosan nanoparticles (PEC-SLN). Since no in-vitro lymphoid tissue is currently available, CaCo-2 cell monolayer could be considered an alternative in vitro model to be used as a ...
TY - JOUR. T1 - Epigallocatechin gallate (EGCG) inhibits the sulfation of 1-naphthol in a human colon carcinoma cell line, Caco-2. AU - Isozaki, T.. AU - Tamura, H. O.. PY - 2001/10/4. Y1 - 2001/10/4. N2 - We have previously reported that epigallocatechin gallate (EGCG) strongly inhibits the in vitro phenol sulfo-transferase (P-ST) activity of a human colon carcinoma cell line, Caco-2. In the present study, we examined the ability of EGCG to inhibit the sulfation of 1-naphthol in intact Caco-2 cells. Sulfation of 1-naphthol was detected in Caco-2 cells after 2 h of incubation, and was observed to continue for 24 h, resulting in an accumulation of sulfated 1-naphthol. Sulfation was strongly inhibited by the addition of EGCG to the culture medium. The IC50 of EGCG was calculated to be 20 μM; this value is similar to that obtained from in vitro assays (14 μM) [Ref. Tamura et al., Biol. Pharm. Bull., 23, 695, (2000)]. These results indicate that catechins are capable of inhibiting P-ST activity in ...
Clostridium perfringens type A is a common source of foodborne illness (FBI) in humans. Vegetative cells sporulate in the small intestinal tract and produce the major pathogenic factor C. perfringens enterotoxin. Although sporulation plays a critical role in the pathogenesis of FBI, the mechanisms inducing sporulation remain unclear. Bile salts were shown previously to induce sporulation, and we confirmed deoxycholate (DCA)-induced sporulation in C. perfringens strain NCTC8239 cocultured with human intestinal epithelial Caco-2 cells. In the present study, we performed transcriptome analyses of strain NCTC8239 in order to elucidate the mechanism underlying DCA-induced sporulation. Of the 2,761 genes analyzed, 333 were up- or downregulated during DCA-induced sporulation and included genes for cell division, nutrient metabolism, signal transduction, and defense mechanisms. In contrast, the virulence-associated transcriptional regulators (the VirR/VirS system, the agr system, codY, and abrB) were ...
Fig. 7 Cytotoxicity of V. parahaemolyticus and recombinants L. rhamnosus strains in CaCo-2 cell line. The figure shows LDH level release as absorbance at 490 nm of V. parahaemolyticus and Lactobacillus strains in colon carcinoma cell line (CaCo-2) monolayers. The strains used were V. parahaemolyticus RIMD 2210633 (RIMD), L. rhamnosus wild type (L. rhamnosus), L. rhamnosus pCWA-MAM-7 (pCWA-MAM7) and L. rhamnosus pSEC-MAM-7 (pSEC-MAM7). As a positive control (Con-trol+), CaCo-2 were treated with Tritón χ 100. The figure represents the result of three full biological replicates, each time in technical triplicate. *p , 0.001 (Students-Test). Discussion. We evaluated the anti-adhesion capacity of L. rhamnosus recombinant strains against V. parahaemolyticus compared to the wild type, in HEp-2 and CaCo-2 epithelial cell lines.. Adhesion of recombinant L. rhamnosus strains was not better than that of L. rhamnosus wild type. In fact, in CaCo-2, an epithelial cell line that mimics epithelial cells of ...
TY - JOUR. T1 - Transport across a polarized monolayer of Caco-2 cells by transferrin receptor-mediated adenovirus transcytosis. AU - Zhu, Zeng B.. AU - Makhija, Sharmila K.. AU - Lu, Baogen. AU - Wang, Minghui. AU - Rivera, Angel A.. AU - Preuss, Meredith. AU - Zhou, Fen. AU - Siegal, Gene P.. AU - Alvarez, Ronald D.. AU - Curiel, David T.. PY - 2004/7/20. Y1 - 2004/7/20. N2 - Adenoviral vectors have a poor record of transgene delivery efficiency through physical barriers such as the epithelium or endothelium. We report here the construction of an adenoviral vector that has the capability to be transported across polarized epithelial monolayers of Caco-2 cells (a colon carcinoma cell line) by transcytosis. This transcytosis is transferrin receptor (TfR)-mediated with use of a bifunctional adaptor, soluble coxsackie adenovirus receptor (sCAR)-Tf, and is both temperature and iron dependent. Under experimental conditions, the adenoviral transcytosis was inhibited by pretreatment of Caco-2 cells ...
Cell lines are invaluable tools for biomedical research. Cancer cell lines are the foundation of cancer biology and the quest for drug treatments. Adenocarcinoma is a cancer of the epithelium (including colon) and originates in glandular tissue, such as skin surface layer and glands.. Product. These human adenocarcinoma cell lines have been previously licensed to commercial parties for research into human adenocarcinoma treatments.. These cell lines are available through Public Health England ECACC. Please make your purchase through the links below.. Imperial Innovations requests that commercial/for profit parties contact them first to discuss the terms of supply prior to Innovations authorising the release of supplies from ECACC.. ...
The effect of processing (homogenization, lyophilization, acid-extraction) meat products on iron uptake from meat combined with uncooked iron-fortified cereal was evaluated using an in vitro digestion/Caco-2 cell model. Beef was cooked, blended to create smaller meat particles, and combined with electrolytic iron-fortified infant rice cereal. Chicken liver was cooked and blended, lyophilized, or acid-extracted, and combined with FeSO4-fortified wheat flour. In the beef-cereal combination, Caco-2 cell iron uptake, assessed by measuring the ferritin formed by cells, was greater when the beef was blended for the greatest amount of time (360 s) compared with 30 s (P , 0·05). Smaller liver particles (blended for 360 s or lyophilized) significantly enhanced iron uptake compared to liver blended for 60 s (P , 0·001) in the liver-flour combination. Compared to liver blended for 60 s, acid-extraction of liver significantly enhanced iron uptake (P = 0·03) in the liver-flour combination. Homogenization ...
Bacterial strain, cell line and culture conditions The strain of S. typhimurium (fim+ve) was obtained from Dr. Philip T. Loverde (State University of New York, Buffalo). Bacteria were grown on Luria agar for 48h at 37 oC. INT-407 (human embryo small intestinal epithelial cell line) was obtained from National Centre for Cell Science (Pune), grown in RPMI medium containing fetal bovine serum (10%), L-glutamine (5mM), streptomycin (100µg/ml) and penicillin (100µg/ml) at 37 oC in a CO2 incubator .. Purification of fimbrial adhesin. The method of Moch et al. [12] (with slight modifications) was used to isolate the fimbrial adhesin of S. typhimurium [12]. Briefly, the bacteria cultured on Luria agar were collected, washed and suspended in 10mM Tris/HCl (pH 7.8) buffer. This was followed by detachment of the fimbriae from the bacteria by omnimixing (10min) at 8-10 oC and centrifugation at 12000 x g for 30min.The supernatant containing fimbriae was further centrifuged (227,000 x g, 2.5h) and the ...
Human enterotoxigenic Escherichia coli adhere to the brush border of human enterocytes. The mean number of bacteria adhering to one enterocyte (adhesion index) varied from 0.5 to 3.1 when the strains produce adhesins. Different factors related to enterocytes and to bacteria are involved in this variability. The number of bacteria which adhered to enterocytes issued from the same donor varied from from 0 to 12. Moreover the proportion of enterocytes on which several bacteria sticked did not exceed 20%. This variability might be due to the disparity in the maturation of the enterocytes. On the other hand, whatever the adhesion factors considered, the adhesion index varied according to the donors. ETEC strains did not express adhesion when bacteria were grown in a liquid medium but this capacity could be restored after transfer on solid medium. This phenomenon seemed like a phase-variation and appeared to be linked to a 4 to 6 kilobases (kb) plasmid. On the other hand, when the bacteria were grown on agar
British Journal of Nutrition 2017.. This paper belongs to a series of three publications that examine the intestinal barrier, its role in health and disease and the potential impact of probiotics on function. In this review, the available evidence for the role of probiotics in epithelial integrity is investigated.. Intestinal barrier integrity is a prerequisite for homeostasis of mucosal function. Evidence is mounting that disruption of epithelial barrier integrity is one of the major etiological factors associated with several gastrointestinal diseases, including infection by pathogens, obesity and diabetes, necrotising enterocolitis (NEC), irritable bowel syndrome (IBS), and inflammatory bowel disease (IBD). The notion that specific probiotic bacterial strains can impact barrier integrity fuelled research in which in vitro cell lines, animal models and clinical trials are employed to assess whether probiotics can revert the diseased state back to homeostasis and health. This review catalogues ...
0113] Free Neu5Gc can be taken up by human epithelial cells from an exogenous source and incorporated into different subcellular fractions. Evidence was presented suggesting that the small amounts of Neu5Gc found in some human tissues originated from dietary sources and showed that human Caco-2 cells (human epithelial cells from a primary colon carcinoma) in culture could metabolically incorporate free Neu5Gc, as determined by a Western blot of a total homogenate, using and anti-Neu5Gc antibody. Increasing incorporation of Neu5Gc was found in the total homogenate fraction of the cells over time, with the highest level reached after incubation with 3 mM Neu5Gc for 3 days. Moreover, Western blotting with an anti Neu5Gc antibody demonstrated metabolic incorporation of Neu5Gc into glycoproteins of these cells. The partitioning of the exogenous Neu5Gc into different subcellular fractions of these cells has now been studied. Prior to feeding, Caco-2 cells were split and cultured in human serum instead ...
The yqiC gene of Salmonella enterica serovar Typhimurium (S. Typhimurium) regulates bacterial growth at different temperatures and mice survival after infection. However, the role of yqiC in bacterial colonization and host immunity remains unknown. We infected human LS174T, Caco-2, HeLa, and THP-1 cells with S. Typhimurium wild-type SL1344, its yqiC mutant, and its complemented strain. Bacterial colonization and internalization in the four cell lines significantly reduced on yqiC depletion. Postinfection production of interleukin-8 and human β-defensin-3 in LS174T cells significantly reduced because of yqiC deleted in S. Typhimurium. The phenotype of yqiC mutant exhibited few and short flagella, fimbriae on the cell surface, enhanced biofilm formation, upregulated type-1 fimbriae expression, and reduced bacterial motility. Type-1 fimbriae, flagella, SPI-1, and SPI-2 gene expression was quantified using real-time PCR. The data show that deletion of yqiC upregulated fimA and fimZ expression and
SGLT1-based oral rehydration solution (ORS) used in the management of acute diarrhoea does not substantially reduce stool output, despite the fact that glucose stimulates the absorption of sodium and water. To explain this phenomenon, we investigated the possibility that glucose might also stimulate anion secretion. Transepithelial electrical measurements, isotope flux measurements in Ussing chambers, fluid movement in isolated ileal sacs and electrical measurements in patch clamp studies were used to study the effect of glucose on active chloride and fluid secretion in mouse small intestinal cells and human Caco-2 cells. Confocal fluorescence laser microscopy and immunohistochemistry measured intracellular changes in calcium, SGLT1 and calcium-activated chloride channel (anoctamin 1) expression. In addition to enhancing active sodium absorption, glucose increased intracellular calcium and stimulated electrogenic chloride secretion. Calcium imaging studies showed increased intracellular calcium ...
TY - JOUR. T1 - miR-30 family controls proliferation and differentiation of intestinal epithelial cell models by directing a broad gene expression program that includes SOX9 and the ubiquitin ligase pathway. AU - Peck,Bailey C.E.. AU - Sincavage,John. AU - Feinstein,Sydney. AU - Mah,Amanda T.. AU - Simmons,James G.. AU - Lund,P. Kay. AU - Sethupathy,Praveen. PY - 2016/7/29. Y1 - 2016/7/29. N2 - Proliferation and differentiation of intestinal epithelial cells (IECs) occur in part through precise regulation of key transcription factors, such as SOX9. MicroRNAs (miRNAs) have emerged as prominent fine-tuners of transcription factor expression and activity. We hypothesized that miRNAs, in part through the regulation of SOX9, may mediate IEC homeostasis. Bioinformatic analyses of the SOX9 3′-UTR revealed highly conserved target sites for nine different miRNAs. Of these, only the miR-30 family members were both robustly and variably expressed across functionally distinct cell types of the murine ...
Search and download thousands of Swedish university dissertations (essays). Full text. Free. Dissertation: Defence capabilities of human intestinal epithelial cells.
Flavonoids, polyphenolic compounds, are abundant in the diet. Animal studies in vivo and in vitro data demonstrated that flavonoids could play a protective role in cancer through inhibition of key enzymes, such as CYPIAl/IA2 and SULTIAI both involved in the initiation of cancer, and inhibition of pathways involved in cell proliferation. The beneficial effects of flavonoids in humans could be limited by poor absorption and extensive presystemic metabolism. This dissertation describes the pathways involved in metabolism of one such flavonoid, chrysin (5,7-dihydroxyflavone), by cultured intestinal Caco-2 cells and hepatic Rep G2 cells. In addition, we examined the inducing effect of chrysin on UDP-glucuronosyltransferases (UOTs) in Caco-2 cells. The general hypothesis of this dissertation is that dietary flavonoids are extensively metabolized in the human intestine and act as inducers of VGTs, increasing the metabolic clearance of colon carcinogens. At concentrations that may be achieved from the ...
Gyphosate is a commonly used nonselective herbicide that inhibits plant growth through interference with the production of essential aromatic amino acids. In vivo studies in mammals with radiolabeled glyphosate have shown that 34% of radioactivity was associated with intestinal tissue 2 h after oral administration. The aim of our research was to investigate the transport, binding, and toxicity of glyphosate to the cultured human intestinal epithelial cell line, Caco‐2, and the rat small intestinal crypt‐derived cell line, ileum epithelial cells‐18 (IEC‐18 ). An in vitro analysis of the transport kinetics of [14C]‐glyphosate showed that 4 h after exposure, approximately 8% of radiolabeled glyphosate moved through the Caco‐2 monolayer in a dose‐dependent manner. Binding of glyphosate to cells was saturable and approximately 4 1011 binding sites/cell were estimated from bound [14C]. Exposure of Caco‐2 cells to ≥10 mg/ml glyphosate reduced transmembrane electrical resistance (TEER) ...
EED results from the chronic inflammatory response to repetitive GI infectious insults, and thus populations living in poverty in the developing world are commonly affected to varying degrees. A general association of the lactulose to mannitol ratio, particularly in relationship to impaired linear growth, has been noted among several groups; however, significant issues remain with the tests performance.5 We provide normative values of the lactulose/rhamnose permeability assay in healthy infants and document evidence for increased intestinal permeability in two cohorts of children from populations in which EED is endemic. These observations suggest that the method described has the potential to provide consistent test performance in the field for the detection of impaired intestinal barrier integrity in EED. Our data also suggest that there was monosaccharide (rhamnose) malabsorption (reduced absolute mass excreted and fractional recovery in urine) in at-risk children from Zambia compared to US ...
Uropathogenic Escherichia coli (UPEC) strains are found in high numbers in the gut of patients with urinary tract infections (UTIs). We hypothesised that in hospitalised patients, UPEC strains might translocate from the gut to the blood stream and that this could be due to the presence of virulence genes (VGs) that are not commonly found in UPEC strains that cause UTI only. To test this, E. coli strains representing 75 dominant clonal groups of UPEC isolated from the blood of hospitalised patients with UTI (urosepsis) (n = 22), hospital-acquired (HA) UTI without blood infection (n = 24) and strains isolated from patients with community-acquired (CA)-UTIs (n = 29) were tested for their adhesion to, invasion and translocation through Caco-2 cells, in addition to the presence of 34 VGs associated with UPEC ...
In this work, we assessed the effects of sinomenine (SN) on intestinal octreotide (OCT) absorption both in Caco-2 cell monolayers and in rats. We also investigated the molecular mechanisms of tight junction (TJ) disruption and recovery by SN-mediated changes in the claudin-1 and protein kinase C (PKC) signaling pathway. The data showed that exposure to SN resulted in a significant decrease in the expression of claudin-1, which represented TJ weakening and paracellular permeability enhancement. Then, the recovery of TJ after SN removal required an increase in claudin-1, which demonstrated the transient and reversible opening for TJ. Meanwhile, the SN-mediated translocation of PKC-α from the cytosol to the membrane was found to prove PKC activation. Finally, SN significantly improved the absolute OCT bioavailability in rats and the transport rate in Caco-2 cell monolayers. We conclude that SN has the ability to enhance intestinal OCT absorption and that these mechanisms are related at least in part to
RT-PCR analysis of ABC, SLC and SLCO drug transporters in human lung epithelial cell models. Journal of Pharmacy and Pharmacology 61 (5) , pp. 583-591. 10.1211/jpp/61.05.0006 ...
In vitro characterization of the intestinal absorption of methylmercury using a Caco-2 cell model.: Methylmercury (CH3Hg) is one of the forms of mercury found i
The Caco-2 permeability assay provides a measure of the permeability of a test compound across the intestinal barrier and its potential for interactions with drug transporters. We provide permeability assays for small molecule formulations such as pharmaceuticals, industrial chemicals and consumer products.
Anatomical charts and models provide a valuable patient education tool. Check out these intestinal models from Medical Device Depot.
Adj. enterocytic Definition : Intestinal absorptive cells. Pathology enterocytic anomalies enterocytic diseases See also cells (...)
Dr Weidmann is a senior lecturer at the School of Pharmacy and Life Sciences. Her research interests include molecular cell changes in response to changing glucose and oxygen concentrations and impact upon disease, the effects of flavonoid antioxidants on the regulation of TRB3 expression in hepato and adipocytes; and the cellular uptake and mechanism of flavanoid absorption in human intestinal cells. ...
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Fig. 7 Cytotoxicity of V. parahaemolyticus and recombinants L. rhamnosus strains in CaCo-2 cell line. The figure shows LDH level release as absorbance at 490 nm of V. parahaemolyticus and Lactobacillus strains in colon carcinoma cell line (CaCo-2) monolayers. The strains used were V. parahaemolyticus RIMD 2210633 (RIMD), L. rhamnosus wild type (L. rhamnosus), L. rhamnosus pCWA-MAM-7 (pCWA-MAM7) and L. rhamnosus pSEC-MAM-7 (pSEC-MAM7). As a positive control (Con-trol+), CaCo-2 were treated with Tritón χ 100. The figure represents the result of three full biological replicates, each time in technical triplicate. *p , 0.001 (Students-Test). Discussion. We evaluated the anti-adhesion capacity of L. rhamnosus recombinant strains against V. parahaemolyticus compared to the wild type, in HEp-2 and CaCo-2 epithelial cell lines.. Adhesion of recombinant L. rhamnosus strains was not better than that of L. rhamnosus wild type. In fact, in CaCo-2, an epithelial cell line that mimics epithelial cells of ...
Background and objectives: In review of traditional Persian medicine (TPM) literature concerning multi drug therapy, a group of medicinal plants that are called convoy drugs, agents which penetrate fast into whole or specific part of the body and accelerate delivery of drugs into specific target has been mentioned. In this study, the inhibitory effect of the aqueous extracts of some selected medicinal plants on P-glycoprotein (P-gp) was assessed in order to determine the possibility of herb-drug interactions. Methods: P-gp inhibitory effect of aqueous extracts (250 µg/mL) from some medicinal plants and verapamile (5 µg/mL) was measured using flow cytometry by Rhodamine 123 (Rh123) in Caco2 cell line. Inhibition percent of each sample (%) was compared with a control group (Caco-2 cell with Rh 123). Results: According to the results pennyroyal, aniseed (p|0.001), celery seed, melon seed and white agaric extract (p|0.01) exhibited the highest Rh123 percent in Caco-2 cells that could be associated with
Literature reports describe kiwi fruit as a food with significant effects on human health, including anti-oxidant and anti-inflammatory activity. Fresh fruit or raw kiwi fruit extracts have been used so far to investigate these effects, but the molecule(s) responsible for these health-promoting activities have not yet been identified. Kissper is a kiwi fruit peptide displaying pore-forming activity in synthetic lipid bilayers, the composition of which is similar to that found in intestinal cells. The objective of this study was to investigate the kissper influence on intestinal inflammation using cultured cells and ex-vivo tissues from healthy subjects and Crohns disease (CD) patients. The anti-oxidant and anti-inflammatory properties of kissper were tested on Caco-2 cells and on the colonic mucosa from 23 patients with CD, by challenging with the lipopolysaccharide from Escherichia coli (EC-LPS) and monitoring the appropriate markers by Western blot and immunofluorescence. EC-LPS challenge ...
Purpose : The cornea efficiently restricts permeability of topically administered drugs into the eye. Currently, permeability of drug candidates is tested across excised animal corneas ex vivo or using in vitro cell culture models. The purpose of this study was to develop an artificial cornea for drug permeability screening in early drug development that more adequately resembles the human cornea. Methods : Several modifications of artificial corneas were prepared using different hydrogel cross-linking and coating techniques. The apparent permeability coefficient (Papp) values of standard reference molecules across artificial corneas were tested using Navicyte vertical diffusion chambers. Permeability characteristics (tightness, dynamic range) of the artificial cornea was assessed, and the Papp values across artificial cornea were correlated with those of ex vivo rabbit corneas and in vitro human corneal epithelial cell (HCE-T) culture model. HCE-T cells were also grown on top of artificial ...
TY - JOUR. T1 - Apical membrane chloride channels in a colonic cell line activated by secretory agonists. AU - Halm, D. R.. AU - Rechkemmer, G. R.. AU - Schoumacher, Robert. AU - Frizzell, R. A.. PY - 1988/1/1. Y1 - 1988/1/1. N2 - We characterized the anion channel responsible for the increase in apical membrane Cl secretion using a model salt-secreting epithelium, the T84 colonic cell line. The adenosine 3,5-cyclic monophosphate (cAMP)-mediated secretagogues, prostaglandin E2, forskolin, and 8-bromo-cAMP, evoked activity of an outwardly rectifying Cl channel in previously quiet cell-attached membrane patches. The channel remained active in excised, inside-out membranes, where its single-channel conductance was 40-45 pS at 0 mV with 160 mM NaCl in pipette and bath. Selectivities were P(Cl)/P(Na) = 50 and for halides I(1.8)/Br(1.4)/Cl(1.0)/F(0.4). This halide sequence illustrates that the ability of various anions to undergo transepithelial secretion is determined by the selectivity of the ...
A high sensitivity reversed-phase HPLC method is presented for the simultaneous determination of marker compounds of paracellular transport (atenolol), transcellular transport (propranolol) and P-gp functionality (talinolol) in the Caco-2 system. The Caco-2 system is presently commonly accepted as an in vitro cell culture model of the intestinal mucosa. A programmed wavelength fluorescence detection method was used to optimise the response of the marker compounds. This marker compound mixture and the corresponding HPLC assay can be used for in house validation of the Caco-2 system or to evaluate simultaneously the effect of test compounds or absorption enhancing strategies on monolayer integrity and P-gp functionality. The method can easily be adapted to determine the concentration of atenolol, propranolol and talinolol in blood, thus allowing to use the same compounds in the in situ rat perfusion system with blood sampling from the mesenteric vein ...
THE INTRACELLULAR TRAFFICKING OF TRANSFERRIN IN CACO-2 CELLS AND ITS IMPLICATION TO TRANSFERRIN-MEDIATED ORAL DRUG DELIVERY by Fariba Norouziyan ________________________________________________________________________ A Dissertation Presented to the FACULTY OF THE GRADUATE SCHOOL UNIVERSITY OF SOUTHERN CALIFORNIA In Partial Fulfillment of the Requirements for the Degree DOCTOR OF PHILOSOPHY (PHARMACEUTICAL SCIENCES) May 2007 Copyright 2007 Fariba Norouziyan ii Dedication This dissertation is dedicated to my family and my husband Keith. iii Acknowledgments I thank my advisor, Dr. Wei-Chiang Shen for years of support. I would like to thank my committee members, prof. Austin Mircheff, Curtis Okamoto, Judy Garner, and Sarah Hamm-Alvarez for their help and guidance. My special thanks to Dr. Sarah Hamm-Alvarez for providing the opportunity for me to use the confocal microscopy. I would also like to thank Mrs. Daisy Shen for her help with in vivo study. Finally, I would like to thank my family who have ...
N-nitroso compounds (NOCs) may be implicated in human colon carcinogenesis, but the toxicological mechanisms involved have not been elucidated. Because it was previously demonstrated that nitrosamines and nitrosamides, representing two classes of NOC, induce distinct gene expression effects in colon cells that are particularly related to oxidative stress, we hypothesized that different radical mechanisms are involved. Using electron spin resonance spectroscopy, we investigated the radical-generating properties of genotoxic NOC concentrations in human colon adenocarcinoma cells (Caco-2). Cells were exposed to nitrosamides (N-methyl-N′-nitro-N-nitrosoguanidine and N-methyl-N-nitrosourea) or nitrosamines (N-nitrosodiethylamine, N-nitrosodimethylamine, N-nitrosopiperidine, and N-nitrosopyrrolidine). Nitrosamines caused formation of reactive oxygen species (ROS) and carbon-centered radicals, which was further stimulated in the presence of Caco-2 cells. N-methyl-N-nitrosourea exposure resulted in a ...
Lactobacillus rhamnosus GG is a bacteria that exists naturally in the body, primarily in the intestines. Lactobacillus rhamnosus GG has been used as a probiotic, or "friendly bacteria," to prevent the growth of harmful bacteria in the stomach and intestines. Lactobacillus rhamnosus GG has been used in alternative...
The entire human body and its many compartments are shielded from their external environments by the barrier function of epithelial cell sheets. The paracellular barrier function of tight junctions (TJs) is critical for maintaining homeostasis in any multicellular organism, especially in the skin and internal organs and at the blood-brain barrier. One of the major components of TJs is a family of adhesive membrane proteins known as claudins. Several members of the claudin family are receptors for the bacterial toxin Clostridium perfringens enterotoxin. This toxin often causes food-borne illness both in humans and animals. Saitoh et al. crystallized a complex between the toxin and a claudin that reveals just how the toxin damages epithelial barriers (see the Perspective by Artursson and Knight).. Science, this issue p. 775; see also p. 716 ...

Absorption and Metabolism Characteristics of Rutin in Caco-2 CellsAbsorption and Metabolism Characteristics of Rutin in Caco-2 Cells

... Xiaofang Zhang,1 Jinhui Song,2 Xiaopeng Shi,1 Shan Miao,3 ... These results suggest that a specific transport system mediates rutin movement across the apical membrane in Caco-2 cells and ... 2Department of Otolaryngology, Shenyang 463 Hospital, Xiao He Yan Road 46, Shenyang, Liaoning 110042, China. 3School of ... beneficial polyphenol rutin were studied by measuring the intracellular accumulation and transport of rutin into Caco-2 cells ...
more infohttps://www.hindawi.com/journals/tswj/2013/382350/abs/

Caco 2 whole cell lysate (ab3950) | AbcamCaco 2 whole cell lysate (ab3950) | Abcam

Caco 2 whole cell lysate Lysates datasheet (ab3950). Abcam offers quality products including antibodies, assays and other ... Cell line: Caco-2 (Human colorectal adenocarcinoma).. Growth media: Minimum essential medium (Eagle) with 2mM L-glutamine, 1.5 ... Cell Biology. Epigenetics. Metabolism. Developmental Biology. By research area. Immunology. Microbiology. Neuroscience. Signal ... Cell and tissue imaging tools. Cellular and biochemical assays. By product type. Proteins and Peptides. Proteomics tools. ...
more infohttps://www.abcam.com/caco-2-whole-cell-lysate-ab3950.html

Immunogold labelling of Listeria monocytogenes virulence-related factors within Caco-2 cells.  - PubMed - NCBIImmunogold labelling of Listeria monocytogenes virulence-related factors within Caco-2 cells. - PubMed - NCBI

Finally, comet-tail pseudopod structures shown to be involved in cell-to-cell passage of Listeria in Caco-2 cells did not ... suggesting that the phagocytic process is primarily host-cell-dependent once it is initiated by the bacterial cell. ... Immunogold labelling of Listeria monocytogenes virulence-related factors within Caco-2 cells.. Quinn F1, Pine L, White E, ... produced by Listeria monocytogenes were primarily cell-wall-associated when the bacterium infected Caco-2 tissue culture cell ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/8140278?dopt=Abstract

Extensive metabolism of the flavonoid chrysin by human Caco-2 and Hep G2 cells.  - PubMed - NCBIExtensive metabolism of the flavonoid chrysin by human Caco-2 and Hep G2 cells. - PubMed - NCBI

Extensive metabolism of the flavonoid chrysin by human Caco-2 and Hep G2 cells.. Galijatovic A1, Otake Y, Walle UK, Walle T. ... Sulphate conjugation occurred at a rate twice that of glucuronic acid conjugation in both cell types. 3. M1 was catalyzed by ... Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, Charleston ... the metabolism of chrysin by cultured intestinal Caco-2 cells and hepatic Hep G2 cells was studied, together modelling human ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/10647910?dopt=Abstract

Myricitrin Inhibits Acrylamide-Mediated Cytotoxicity in Human Caco-2 Cells by Preventing Oxidative StressMyricitrin Inhibits Acrylamide-Mediated Cytotoxicity in Human Caco-2 Cells by Preventing Oxidative Stress

W. Chen, L. Feng, H. Nie, and X. Zheng, "Andrographolide induces autophagic cell death in human liver cancer cells through ... Myricitrin Inhibits Acrylamide-Mediated Cytotoxicity in Human Caco-2 Cells by Preventing Oxidative Stress. Wei Chen,1 Lina Feng ... W. Chen, Z. Zhao, L. Li et al., "Hispolon induces apoptosis in human gastric cancer cells through a ROS-mediated mitochondrial ... J. Cao, Y. Liu, L. Jia et al., "Curcumin attenuates acrylamide-induced cytotoxicity and genotoxicity in HepG2 cells by ROS ...
more infohttps://www.hindawi.com/journals/bmri/2013/724183/ref/

Caco-2 Vs Hybridoma cells - Cell BiologyCaco-2 Vs Hybridoma cells - Cell Biology

Hybridoma cell line has a doubling time of just 24hrs is growing fast and soon will replace Caco-2 cells. caco-2 cells are ... Top : Forum Archives: : Cell Biology. Caco-2 Vs Hybridoma cells - contamination of Caco-2 cell line with hybridoma cell line ( ... hi friends ! iam handling few cell lines ! we recently bought Caco-2 cell line. i have ended up mixing caco-2 cells with ... can any one help me out to separate these cells so that i can save Caco-2 cell line. ...
more infohttp://www.protocol-online.org/biology-forums/posts/15068.html

Determination of Autophagy in the Caco-2 Spontaneously Differentiating Model of Intestinal Epithelial Cells | SpringerLinkDetermination of Autophagy in the Caco-2 Spontaneously Differentiating Model of Intestinal Epithelial Cells | SpringerLink

These cells, upon reaching confluency, spontaneously differentiate into... ... The Caco-2 colorectal cancer cell line is widely used as a model for intestinal differentiation and barrier function. ... Cell 140:313-326. doi: 10.1016/j.cell.2010.01.028 CrossRefPubMedPubMedCentralGoogle Scholar ... Cell 140:313-326. doi: 10.1016/j.cell.2010.01.028 CrossRefPubMedPubMedCentralGoogle Scholar ...
more infohttps://link.springer.com/protocol/10.1007/7651_2017_66

The proliferations of Caco-2, MCF-7, and HELA cells were moreThe proliferations of Caco-2, MCF-7, and HELA cells were more

... inhibited when treated with water-soluble extract of fermented ... The proliferations of Caco-2, MCF-7, and HELA cells were more inhibited when treated with WSE of fermented camel milk extracts. ... 2-azino-bis(3-ethylbenzo-thiazoline-6-sulphonic acid). The highest angiotensin-converting enzyme inhibition of WSE in camel ... Camel milk fermented byLc.K782 had the highest antioxidant activity by 2, ...
more infohttp://www.greenmedinfo.com/article/proliferations-caco-2-mcf-7-and-hela-cells-were-more-inhibited-when-treated-wa

Modulation of Cl-/OH- exchange activity in Caco-2 cells by nitric oxide.Modulation of Cl-/OH- exchange activity in Caco-2 cells by nitric oxide.

... exchange activity in human Caco-2 cells. Our results demonstrate that NO inhi ... Previous Document: Vitamin D-inducible calcium transport and gene expression in three Caco-2 cell lines.. Next Document: LPS- ... Caco-2 Cells. Cyclic AMP / analogs & derivatives*, pharmacology. Cyclic AMP-Dependent Protein Kinases / antagonists & ... Our data in support of this conclusion can be outlined as follows: 1) incubation of Caco-2 cells with SNAP (500 microM) for 30 ...
more infohttp://www.biomedsearch.com/nih/Modulation-Cl-exchange-activity-in/12181176.html

Cytotoxicity of peptide-coated silver nanoparticles on the human intestinal cell line Caco-2.Cytotoxicity of peptide-coated silver nanoparticles on the human intestinal cell line Caco-2.

In order to investigate the effects of silver nanoparticles on human intestinal cells, we used the Caco-2 cell line and peptide ... Cell viability and proliferation were measured using Promegas CellTiter-Blue(®) Cell Viability assay, DAPI staining and ... 22615588 - Molecular analysis of men1 expression in mcf7, t47d and mda-mb 468 breast cancer cell l.... 1576428 - Primary b-cell ... Exposure of proliferating Caco-2 cells to silver nanoparticle induced decreasing adherence capacity and cytotoxicity, whereby ...
more infohttp://www.biomedsearch.com/nih/Cytotoxicity-peptide-coated-silver-nanoparticles/22418598.html

Toxins | Free Full-Text | Dual Effects Exerted in Vitro by Micromolar Concentrations of Deoxynivalenol on Undifferentiated Caco...Toxins | Free Full-Text | Dual Effects Exerted in Vitro by Micromolar Concentrations of Deoxynivalenol on Undifferentiated Caco...

A 1.5 μM concentration of DON maintained cell adherence of non-proliferating Caco-2 cells, whilst arresting the growth of ... actively proliferating cells compared with control Caco-2 cells in vitro. At 0.37 μM, DON enhanced Caco-2 cell metabolism, ... thereby triggering a moderate increase in cell proliferation. The results of the current study suggested that low ... on the proliferation of undifferentiated Caco-2 cells presenting a tumorigenic phenotype. ...
more infohttp://www.mdpi.com/2072-6651/7/2/593

Chitosan binding onto the epigallocatechin-loaded ferritin nanocage enhances its transport across Caco-2 cells - Food &...Chitosan binding onto the epigallocatechin-loaded ferritin nanocage enhances its transport across Caco-2 cells - Food &...

... encapsulated ferritin cage across the Caco-2 cells was investigated. After the encapsulation of EGC in apo-red bean (adzuki) ... Furthermore, the transport of EGC in ERCs980 and ERCs4600 across the Caco-2 cells was enhanced by the transferrin receptor 1 ( ... Chitosan binding onto the epigallocatechin-loaded ferritin nanocage enhances its transport across Caco-2 cells R. Yang, J. Tian ... Chitosan binding onto the epigallocatechin-loaded ferritin nanocage enhances its transport across Caco-2 cells ...
more infohttps://pubs.rsc.org/en/content/articlelanding/2018/fo/c8fo00097b

What does caco-2 cells mean?What does caco-2 cells mean?

Meaning of caco-2 cells. What does caco-2 cells mean? Information and translations of caco-2 cells in the most comprehensive ... Definition of caco-2 cells in the Definitions.net dictionary. ... Search for caco-2 cells on Amazon. *Search for caco-2 cells on ... These cells are valuable in vitro tools for studies related to intestinal cell function and differentiation. ... Human colonic ADENOCARCINOMA cells that are able to express differentiation features characteristic of mature intestinal cells ...
more infohttps://www.definitions.net/definition/caco-2%20cells

Using Caco-2 Cells to Study Lipid Transport by the Intestine | Protocol (Translated to Chinese)Using Caco-2 Cells to Study Lipid Transport by the Intestine | Protocol (Translated to Chinese)

... 这的Caco-2模型提供在其他的Caco-2机型12最有效的脂质运输- 14。然而, 在体内的淋巴插管模型仍然比任何体外模型更有效地输送脂质。的根本原因已被最近讨论8,即因为Caco-2细胞产生2个不同的载脂
more infohttps://www.jove.com/video/53086/caco-2?language=Chinese

Evaluation of metallothionein formation as a proxy for zinc absorption in an in vitro digestion/Caco-2 cell culture model -...Evaluation of metallothionein formation as a proxy for zinc absorption in an in vitro digestion/Caco-2 cell culture model -...

... absorption in a cell culture model. The MT intracellular concentration was determined using a cadmium/hemoglobin affinity assay ... Caco-2 cell metallothionein (MT) formation was studied to determine if MT could be used as a proxy for zinc (Zn) ... The Zn and MT concentrations in the cells incubated with Zn (when ,10 μmol L−1) were similar to the untreated cells. PA ... Cells were treated with a media containing 0, 2, 5, 10, 25, 50, 75 μmol L−1 Zn (ZnCl2). The effect of varying the Zn : PA molar ...
more infohttp://pubs.rsc.org/en/Content/ArticleLanding/2012/FO/C2FO10232C

In Vitro Permeability Across Caco-2 Cells (Colonic) Can Predict In Vivo (Small Intestinal) Absorption in Man-Fact or Myth |...In Vitro Permeability Across Caco-2 Cells (Colonic) Can Predict In Vivo (Small Intestinal) Absorption in Man-Fact or Myth |...

To evaluate and optimize the use of Caco-2 cell monolayers to predict the in vivo absorption of a broad range of compounds in ... Caco-2 absorption prediction human P-glycoprotein transport This is a preview of subscription content, log in to check access. ... To evaluate and optimize the use of Caco-2 cell monolayers to predict thein vivo absorption of a broad range of compounds in ... Caco-2 cells are derived from human adenocarcinoma colon cells and spontaneously differentiate when grown on porous ...
more infohttps://link.springer.com/article/10.1023%2FA%3A1012102522787

Evaluation of the Intestinal Transport of a Phenylethanoid Glycoside-Rich Extract from Cistanche deserticola across the Caco-2...Evaluation of the Intestinal Transport of a Phenylethanoid Glycoside-Rich Extract from Cistanche deserticola across the Caco-2...

... from CD as an integrated system in the Caco-2 cell monolayer model using a bioassay system. The results showed that PRE is ... Cell differentiation Is the Subject Area "Cell differentiation" applicable to this article? Yes. No. ... Caco-2 cells Is the Subject Area "Caco-2 cells" applicable to this article? Yes. No. ...
more infohttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0116490

Toxins | Free Full-Text | Screening of Cytotoxic B. cereus on Differentiated Caco-2 Cells and in Co-Culture with Mucus...Toxins | Free Full-Text | Screening of Cytotoxic B. cereus on Differentiated Caco-2 Cells and in Co-Culture with Mucus...

The B. cereus cell-free supernatants have been tested for their toxicity in vitro, on differentiated (21 day-old) Caco-2 cells ... Caco-2 cells) and a co-culture (Caco-2 and HT29-MTX mucus-secreting cells) model has been performed with selected B. cereus ... The B. cereus cell-free supernatants have been tested for their toxicity in vitro, on differentiated (21 day-old) Caco-2 cells ... Keywords: B. cereus; enterotoxin; cytotoxicity; Caco-2; HT29-MTX B. cereus; enterotoxin; cytotoxicity; Caco-2; HT29-MTX ...
more infohttps://www.mdpi.com/2072-6651/8/11/320

Identification of Host Cell Factors Associated with Astrovirus Replication in Caco-2 Cells | Journal of VirologyIdentification of Host Cell Factors Associated with Astrovirus Replication in Caco-2 Cells | Journal of Virology

Virus-Cell Interactions. Identification of Host Cell Factors Associated with Astrovirus Replication in Caco-2 Cells. Andrea ... Cells, virus, and reagents.Cells of the colon adenocarcinoma Caco-2 cell line, obtained from the American Type Culture ... Identification of host cell factors associated with astrovirus replication in Caco-2 cells. J Virol 89:10359-10370. doi:10.1128 ... The biophysics and cell biology of lipid droplets. Nat Rev Mol Cell Biol 14:775-786. doi:10.1038/nrm3699. ...
more infohttps://jvi.asm.org/content/89/20/10359

Glutathione and thioredoxin redox during differentiation in human colon epithelial (Caco-2) cells | Gastrointestinal and Liver...Glutathione and thioredoxin redox during differentiation in human colon epithelial (Caco-2) cells | Gastrointestinal and Liver...

Cell culture.. The human colon cancer cell line, Caco-2, was obtained from the American Type Culture Collection (Rockville, MD ... Cell cycle analysis.. Cell cycle analysis was performed to further elucidate Caco-2 growth characteristics. A significant ... Cell cycle and apoptosis measurements.. Cells were trypsinized and fixed with 100% ethanol at room temperature. Fixed cells ... There was a significant decrease in the percentage of cells in the synthesis (S) phase (B) of the cell cycle as cells ...
more infohttp://ajpgi.physiology.org/content/283/6/G1352

Influence of K-ras Activation on the Survival Responses of Caco-2 Cells to the Chemopreventive Agents Sulindac and...Influence of K-ras Activation on the Survival Responses of Caco-2 Cells to the Chemopreventive Agents Sulindac and...

... ras-transfected cells to 5 mm DFMO had a much more pronounced effect on cell survival than that exhibited by the parental Caco- ... Preparation of Whole-Cell Lysates of Caco-2 Cells.. Whole-cell lysates of Caco-2 cells were prepared according to commercially ... Cell Culture and Drug Treatments.. Caco-2 cells were obtained from the American Type Culture Collection (Rockville, MD). Caco-2 ... Cell Number and Viability Determinations.. Caco-2 cells were removed from the monolayer by treatment with trypsin (∼1500 units/ ...
more infohttp://cebp.aacrjournals.org/content/9/11/1155

L-arginine attenuates Interleukin-1β (IL-1β) induced Nuclear Factor Kappa-Beta (NF-κB) activation in Caco-2 cellsL-arginine attenuates Interleukin-1β (IL-1β) induced Nuclear Factor Kappa-Beta (NF-κB) activation in Caco-2 cells

Methods Caco-2 cells were cultured, transfected with a NF-κB promoter luciferase vector, incubated ± L-Arg, ± IL-1β and ... Conclusion The inhibitory effects of L-Arg on IL-1β-mediated NF-κB-activation in Caco-2 cells involve L-Arg transport activity ... NO production and iNOS inhibitor on IL-1β-mediated NF-κB-activation in Caco-2 cells. ...
more infohttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0174441

CiNii 論文 - 
 		
 		
 			
 		 	
 		 		
 		 			Effects of Functional Food Constituents on P-glycoprotein Activity in Caco-2 Cells...CiNii 論文 - Effects of Functional Food Constituents on P-glycoprotein Activity in Caco-2 Cells...

Curcuma drugs and curcumin regulate the expression and function of P-gp in Caco-2 cells in completely opposite ways HOU XL ... Determination of the Effects of Green Tea Extract and Fruit Extracts on P-glycoprotein Activity in Caco-2 Cells by Using a New ... Chrysin blocks topotecan-induced apoptosis in Caco-2 cells in spite of inhibition of ABC-transporters SCHUMACHER M ... Effects of Dietary Ingredients on Function and Expression of P-Glycoprotein in Human Intestinal Epithelial Cells Okura Takashi ...
more infohttps://ci.nii.ac.jp/naid/10030158827

AID 679077 - TP TRANSPORTER: transepithelial transport of Rhodamine 123 (basal to apical) in Caco-2 cells - PubChemAID 679077 - TP TRANSPORTER: transepithelial transport of Rhodamine 123 (basal to apical) in Caco-2 cells - PubChem

BioAssay record AID 679077 submitted by ChEMBL: TP_TRANSPORTER: transepithelial transport of Rhodamine 123 (basal to apical) in Caco-2 cells.
more infohttps://pubchem.ncbi.nlm.nih.gov/bioassay/679077

Transepithelial transport of oral cephalosporins by monolayers of intestinal epithelial cell line Caco-2: specific transport...Transepithelial transport of oral cephalosporins by monolayers of intestinal epithelial cell line Caco-2: specific transport...

Transepithelial transport of oral cephalosporins by monolayers of intestinal epithelial cell line Caco-2: specific transport ... Transepithelial transport of oral cephalosporins by monolayers of intestinal epithelial cell line Caco-2: specific transport ... Transepithelial transport of oral cephalosporins by monolayers of intestinal epithelial cell line Caco-2: specific transport ... Transepithelial transport of oral cephalosporins by monolayers of intestinal epithelial cell line Caco-2: specific transport ...
more infohttp://jpet.aspetjournals.org/content/261/1/195
  • Although derived from a colon ( large intestine ) carcinoma , when cultured under specific conditions the cells become differentiated and polarized such that their phenotype , morphologically and functionally, resembles the enterocytes lining the small intestine . (wikipedia.org)
  • A downregulation of FASN effectively inhibits the activity of "HER2-PI3K/Akt axis" and alters the malignant phenotype in colorectal cancer cells. (biomedcentral.com)
  • 1 It was shown that intestinal epithelial cells (IEC) may change phenotype as a consequence of stimulation by IEL derived soluble mediators such as interferon (IFN) γ. (bmj.com)
  • These results suggest that a specific transport system mediates rutin movement across the apical membrane in Caco-2 cells and that metabolic enzymes are important for this process. (hindawi.com)
  • GG reduced AFB 1 uptake and protected against both membrane and DNA damage in the Caco-2 model. (asm.org)
  • Cookson and Nataro ( 10 ) observed that attachment of Afa/Dr DAEC onto epithelial Hep-2 cells is followed by induction of a long thin membrane extending from the cell surface. (asm.org)
  • The hallmark of metastatic cells is their ability to invade through the basement membrane and migrate to other parts of the body. (corning.com)
  • From cell culture reagents such as Matrigel high concentration to membrane insert systems, like Falcon® permeable supports , discover how Corning cell culture products can help you gain greater insights into tumor cell growth, morphology, and migration. (corning.com)
  • Furthermore, CPE action in host cells is complex, involving the toxin binding to receptors, receptor-bound CPE oligomerizing into a prepore on the membrane surface, and β-hairpins in the CPE prepore inserting into the membrane to form a pore that induces cell death. (cloudfront.net)
  • Imaging conditions were optimized, including evaluation of suitable membrane materials and media solutions, as well as tolerable laser powers for non-destructive imaging of the live cells. (helsinki.fi)
  • Of the possible physiological stimuli occurring during feeding, insulin was thought to be a candidate because it exerts systemic hypoglycemic effects by stimulating the translocation of GLUT4 into the plasma membrane of skeletal muscle and adipose cells (rev. in 12 ) and decreasing liver glucose output. (diabetesjournals.org)
  • In Caco2 cells, GLUT12 translocation to the apical membrane and α‐methyl‐\nd‐glucose uptake by the transporter are stimulated by protons, glucose, insulin, tumor necrosis factor‐α (TNF‐α), protein kinase C, and AMP‐activated protein kinase. (metatoc.com)
  • Sucrose esters fluidized the plasma membrane and enhanced the accumulation of efflux transporter ligands rhodamine 123 and calcein AM in epithelial cells, but did not inhibit the P-glycoprotein (P- gp)-mediated calcein AM accumulation in MES-SA/Dx5 cell line. (mtak.hu)
  • For that purpose, the characteristics of the uptake of 1-methyl-4-phenylpyridinium, a model organic cation, at the brush-border membrane of Caco-2 cells were compared with those of the extraneuronal monoamine transporter (EMT)-mediated transport. (antibody-antibodies.com)
  • In conclusion, our results suggest that uptake of organic cations at the brush-border membrane of Caco-2 cells may occur through two distinct Na+-independent transporters belonging to the ASF family: hEMT and hOCT1. (antibody-antibodies.com)
  • The intestinal absorption and metabolism characteristics of the potentially beneficial polyphenol rutin were studied by measuring the intracellular accumulation and transport of rutin into Caco-2 cells with the sensitive and reliable analytical method of HPLC-coupled tandem mass spectrometry. (hindawi.com)
  • As such, these cells represent a useful and well-accepted tool for metabolism and transport studies (absorption, permeability and/or secretion of drugs/chemicals across the intestinal mucosa). (abcam.com)
  • Extensive metabolism of the flavonoid chrysin by human Caco-2 and Hep G2 cells. (nih.gov)
  • In an effort better to understand factors that may affect the oral bioavailability of the bioflavonoids from dietary sources, the metabolism of chrysin by cultured intestinal Caco-2 cells and hepatic Hep G2 cells was studied, together modelling human presystemic metabolism. (nih.gov)
  • Intracellular GSH levels are higher in actively proliferating cells ( 7 , 34 ) and decrease gradually during serum starvation as cells become quiescent ( 34 ). (physiology.org)
  • Listeria monocytogenes being a facultative intracellular bacterium can enter, survive and multiply in both phagocytic and non-phagocytic cells and this property of the bacterium has been extensively studied and understood. (bio-protocol.org)
  • Thus human intestinal cells are used as an in vitro medium to study adhesion and intracellular survival of Listeria monocytogenes . (bio-protocol.org)
  • In this study, isolates were compared quantitatively in established assays with the enterocyte-like cell line, Caco-2, to determine the extent to which intracellular invasion contributes to translocation across epithelial cell monolayers, and whether isolates vary in this respect. (microbiologyresearch.org)
  • Coadministration of tenofovir, GCV, or Allo decreased the amounts of intracellular ddI breakdown products in CEM cells, while they increased the ddI concentrations (twofold increase with each drug at approximately 20 μM). (pubmedcentralcanada.ca)
  • To gain more knowledge of GLUT12 function in the intestine, we investigated GLUT12 subcellular localization in the small intestine and its regulation by sugars, hormones, and intracellular mediators in Caco2 cells and mice. (metatoc.com)
  • Cobalt, an inhibitor of the prolyl hydroxylase enzymes governing HIF-1α breakdown in the cell, induces transepithelial leakiness in CaCo-2 BBe cell layers in a time and concentration-dependent manner. (springermedizin.de)
  • Less-differentiated cell layers were significantly more affected than well-differentiated cell layers regarding induced transepithelial leak. (springermedizin.de)
  • The addition of the antioxidant, superoxide dismutase, significantly reversed the bactericidal activity of both Caco-2 cells and neutrophils for P. aeruginosa and S. aureus, while catalase had no effect. (ovid.com)
  • Over the years, the characteristics of the cells used in different laboratories around the world have diverged significantly, which makes it difficult to compare results across labs. (wikipedia.org)
  • Exposure to 0.05 mM NE, but not to higher concentrations, also resulted in significantly more spirochaete cells entering capillary tubes containing 4% porcine gastric mucin than occurred with untreated cultures. (edu.au)
  • 2. The uptake of [ 3 H]-VBL by Caco-2 cells was significantly increased by the ethyl acetate extract of GFJ as well as by cyclosporin A. The extract was separated on a Cosmosil column and the eluate with 60% methanol increased [ 3 H]-VBL uptake, while the activity to inhibit CYP3A4 was greatest in the 70 and 80% eluates. (elsevier.com)
  • In vitro peripheral blood mononuclear cell stimulation by F. prausnitzii led to significantly lower IL-12 and IFN-γ production levels and higher secretion of IL-10. (pnas.org)
  • Results show that both viscosity grade MCCs managed to significantly decrease the TEER of Caco-2 cell monolayers when they were applied apically at concentrations of 3-5% (w/v). Transport studies with Caco-2 cells revealed substantial increases of LMWH permeation in the presence of both viscosity grade MCCs compared with controls. (mendeley.com)
  • Sucrose esters in nontoxic concentrations significantly reduced resistance and impedance, and increased permeability for atenolol, fluorescein, vinblastine, and rhodamine 123 in Caco-2 monolayers. (mtak.hu)
  • Uptake of [3H]MPP+ by Caco-2 and 293hEMT cells showed pH-dependence: it was significantly reduced (to 86% and 62% of control, respectively) when the pH of the extracellular medium was decreased to 6.2, and increased (to 116% and 136% of control, respectively) when the extracellular pH was increased to 8.2. (antibody-antibodies.com)
  • Heat shock stress ameliorates cytokine mixture-induced permeability by downregulating the nitric oxide and signal transducer and activator of transcription pathways in Caco-2 cells. (semanticscholar.org)
  • Our findings suggest that either heat-killed probiotics or probiotic cell wall extracts are able to attenuate inflammation through pathways similar to that of live bacteria. (beds.ac.uk)
  • The accumulation of genetic and epigenetic alterations mediates CRC formation and progression by deregulating key signaling pathways in cancer cells. (nature.com)
  • Transepithelial electrical resistance and transepithelial diffusion of 14 C-D-mannitol and other radiolabeled probes are used as indicators of transepithelial barrier function of CaCo-2 BBe human gastrointestinal epithelial cell layers cultured on permeable supports. (springermedizin.de)
  • A genetically modified CaCo-2 variant with reduced levels of HIF-1β, showed reduced transepithelial leak in response to cobalt exposure, further indicating that elevation of HIF-1α levels induced by agents of "chemical hypoxia" is responsible for the compromised barrier function of the CaCo-2 BBe cell layers. (springermedizin.de)
  • In Caco-2 cells, myrrh induced an increase in transepithelial resistance (TER) which was associated with downregulation of the channel-forming tight junction (TJ) protein claudin-2 via inhibition of the PI3 kinase signalling pathway. (springermedizin.de)
  • These MCCs were tested on Caco-2 cells for their efficiency to decrease the transepithelial electrical resistance (TEER) and to increase the paracellular permeability of the anionic macromolecular anticoagulant low molecular weight heparin (LMWH). (mendeley.com)
  • In the present study, fully differentiated Caco-2 cell monolayers were used to evaluate the effects of LA (1-50 mmol/L) on the cellular retention and transepithelial transport of soluble non-heme iron (as ferric nitrilotriacetate). (chalmers.se)
  • We conclude that LA reduces cellular retention and transepithelial transport of Fe(l I) by Caco-2 cells in a dose-dependent manner. (chalmers.se)
  • 5 Confocal microscopy was performed only after the transepithelial electrical resistance had risen to more than 250 Ω/cm 2 . (ashpublications.org)
  • This confirmed the formation of glucuronide conjugates and the hydroxylation of ZON during the incubation with Caco-2 cells. (ugent.be)
  • Exposure of B. pilosicoli cultures to 0.05 mM NE prior to incubation with Caco-2 monolayers resulted in more attachment to the monolayer than occurred with non-exposed cultures. (edu.au)
  • Sulfation of 1-naphthol was detected in Caco-2 cells after 2 h of incubation, and was observed to continue for 24 h, resulting in an accumulation of sulfated 1-naphthol. (elsevier.com)
  • Uptake of [3H]MPP+ by Caco-2 cells and 293hEMT cells showed potential-dependence: substitution of KCl for NaCl in the incubation medium resulted in a reduction in the inward transport of [3H]MPP+ (to 70% and 40% of control, respectively). (antibody-antibodies.com)
  • Uptake of [3H]MPP+ by Caco-2 and 293hEMT cells showed only little dependence on Na+: substitution of NaCl of the incubation media with LiCl resulted in a small decrease (of 19% and 14%, respectively) in [3H]MPP+ uptake. (antibody-antibodies.com)
  • Finally, comet-tail pseudopod structures shown to be involved in cell-to-cell passage of Listeria in Caco-2 cells did not possess detectable Listeria antigens on their anterior surface or within their structure, suggesting that the phagocytic process is primarily host-cell-dependent once it is initiated by the bacterial cell. (nih.gov)
  • To evaluate whether a glycosylphosphatidylinositol (GPI) anchor can function as a protein sorting signal in polarized intestinal epithelial cells, the GPI-attachment sequence from Thy-1 was fused to bacterial endoglucanase E' (EGE') from Clostridium thermocellum and polarity of secretion of the chimeric EGE'-GPI protein was evaluated. (biologists.org)
  • 1990 ). Eukaryotic and prokaryotic signal peptides direct secretion of a bacterial endoglucanase by mammalian cells. (biologists.org)
  • Intestinal epithelial cells have receptors that recognize bacterial antigens and in some circumstances are actively involved in bacterial internalization. (ovid.com)
  • We proposed that live/ heat-killed Lactobacillus plantarum MYL26 and bacterial cell wall extract treatments impaired TLR4-NFκb signal transduction through Tollip , SOCS-1 and SOCS-3 activation, thus inducing LPS tolerance. (beds.ac.uk)
  • The increase in glucose accumulation was diminished by heat-denaturing the supernatant, indicating the response of Caco-2 cells is triggered by as yet unknown heat labile bacterial metabolites, not by a reduction in CDM components that decrease glucose uptake. (beds.ac.uk)
  • The influences of pathogens and beneficial bacteria on epithelial cells can be mediated by direct bacteria-cell contacts or indirectly via bacterial metabolites, such as toxins from pathogens [e.g., cholera toxin, E. coli heat stabile toxin) and short chain fatty acids from commensal bacteria (e.g., butyrate). (beds.ac.uk)
  • Reactivity of IEC to bacterial signals may depend on interactions with immunocompetent cells. (bmj.com)
  • CaCO-2 cells alone remained hyporesponsive to the bacterial challenge. (bmj.com)
  • The commonly accepted view that enteroinvasive bacteria enter cells of the intestinal epithelial lining through the apical surface can be challenged in the case of shigellosis. (asm.org)
  • In these experiments, the few bacteria that interacted with the apical surface either bound to microvilli of the cell dome without causing detectable alteration or bound at the level of intercellular junctions at which they demonstrated a limited capacity for paracellular invasion, which permitted subsequent entry through the lateral domain of the cells. (asm.org)
  • When investigating the mechanism of pathogenicity of the diarrheagenic Afa/Dr DAEC strain C1845 in Caco-2 cells, we reported that the first event in cell infection is adhesin binding to the apical surface of polarized cells resulting from recognition of the brush border-associated CD55 ( 2 , 33 ). (asm.org)
  • 1992 ). Expression and polarized apical secretion in Madin-Darby canine kidney cells of a recombinant soluble form of neutral endopeptidase lacking the cytosolic and transmembrane domains. (biologists.org)
  • When looking at Caco-2 cell cultures microscopically, it is evident even by visual inspection that the cells are heterogeneous. (wikipedia.org)
  • We have used coherent anti-Stokes Raman scattering (CARS) microscopy as a novel and rapid, label-free and non-destructive imaging method to gain structural insights into live intestinal epithelial cell cultures used for drug permeability testing. (helsinki.fi)
  • CARS microscopy was shown to be well suited for such analysis with the potential for in situ imaging of the same individual cell-cultures that are used for permeation studies. (helsinki.fi)
  • In the present study, we undertook to further characterize cell injuries following the interaction of wild-type Afa/Dr DAEC strains C1845 and IH11128 expressing fimbrial F1845 adhesin and Dr hemagglutinin, respectively, with polarized, fully differentiated Caco-2/TC7 cells. (asm.org)
  • Leucocyte sensitised CaCO-2 cells produced TNF-α and IL-1β whereas IL-10 was exclusively secreted by human peripheral blood mononuclear cells. (bmj.com)