Butyrate Response Factor 1: A ZINC FINGER MOTIF containing immediate early protein that is closely related to TRISTETRAPROLIN. It interacts with and helps destabilize VASCULAR ENDOTHELIAL GROWTH FACTOR mRNA.Butyrates: Derivatives of BUTYRIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxypropane structure.Serum Response Factor: A MADS domain-containing transcription factor that binds to the SERUM RESPONSE ELEMENT in the promoter-enhancer region of many genes. It is one of the four founder proteins that structurally define the superfamily of MADS DOMAIN PROTEINS.Butyric Acid: A four carbon acid, CH3CH2CH2COOH, with an unpleasant odor that occurs in butter and animal fat as the glycerol ester.RNA Stability: The extent to which an RNA molecule retains its structural integrity and resists degradation by RNASE, and base-catalyzed HYDROLYSIS, under changing in vivo or in vitro conditions.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Tristetraprolin: A ZINC FINGER MOTIF containing transcription factor that was originally identified as one of the IMMEDIATE-EARLY PROTEINS. It shuttles between the CYTOPLASM and the CELL NUCLEUS and is involved in destabilization of mRNAs for TUMOR NECROSIS FACTOR-ALPHA.Endocrinology: A subspecialty of internal medicine concerned with the metabolism, physiology, and disorders of the ENDOCRINE SYSTEM.Heterogeneous-Nuclear Ribonucleoprotein D: A heterogeneous-nuclear ribonucleoprotein that has specificity for AU-rich elements found in the 3'-region of mRNA and may play a role in RNA stability. Several isoforms of hnRNP D protein have been found to occur due to alternative mRNA splicing (RNA SPLICING).Hazard Analysis and Critical Control Points: A system of safety management (abbreviated HACCP) applied mainly to the food industry. It involves the analysis and control of biological, chemical, and physical hazards, from raw material production, procurement and handling, to manufacturing, distribution and consumption of finished products.RNA-Binding Proteins: Proteins that bind to RNA molecules. Included here are RIBONUCLEOPROTEINS and other proteins whose function is to bind specifically to RNA.Exosome Multienzyme Ribonuclease Complex: An intracellular ribonucleolytic protein complex that participates in POSTRANSCRIPTIONAL RNA PROCESSING and RNA DEGRADATION.Olivopontocerebellar Atrophies: A group of inherited and sporadic disorders which share progressive ataxia in combination with atrophy of the CEREBELLUM; PONS; and inferior olivary nuclei. Additional clinical features may include MUSCLE RIGIDITY; NYSTAGMUS, PATHOLOGIC; RETINAL DEGENERATION; MUSCLE SPASTICITY; DEMENTIA; URINARY INCONTINENCE; and OPHTHALMOPLEGIA. The familial form has an earlier onset (second decade) and may feature spinal cord atrophy. The sporadic form tends to present in the fifth or sixth decade, and is considered a clinical subtype of MULTIPLE SYSTEM ATROPHY. (From Adams et al., Principles of Neurology, 6th ed, p1085)Exoribonucleases: A family of enzymes that catalyze the exonucleolytic cleavage of RNA. It includes EC 3.1.13.-, EC 3.1.14.-, EC 3.1.15.-, and EC 3.1.16.-. EC 3.1.-Search Engine: Software used to locate data or information stored in machine-readable form locally or at a distance such as an INTERNET site.Databases, Genetic: Databases devoted to knowledge about specific genes and gene products.Exosomes: A type of extracellular vesicle, containing RNA and proteins, that is secreted into the extracellular space by EXOCYTOSIS when MULTIVESICULAR BODIES fuse with the PLASMA MEMBRANE.Genome, Human: The complete genetic complement contained in the DNA of a set of CHROMOSOMES in a HUMAN. The length of the human genome is about 3 billion base pairs.Biodegradation, Environmental: Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.Linuron: A selective pre- and post-emergence herbicide. (From Merck Index, 11th ed)Physiological Phenomena: The functions and properties of living organisms, including both the physical and chemical factors and processes, supporting life in single- or multi-cell organisms from their origin through the progression of life.Metabolic Networks and Pathways: Complex sets of enzymatic reactions connected to each other via their product and substrate metabolites.Physiological Processes: The functions and activities of living organisms that support life in single- or multi-cellular organisms from their origin through the progression of life.Computational Biology: A field of biology concerned with the development of techniques for the collection and manipulation of biological data, and the use of such data to make biological discoveries or predictions. This field encompasses all computational methods and theories for solving biological problems including manipulation of models and datasets.Knowledge Bases: Collections of facts, assumptions, beliefs, and heuristics that are used in combination with databases to achieve desired results, such as a diagnosis, an interpretation, or a solution to a problem (From McGraw Hill Dictionary of Scientific and Technical Terms, 6th ed).Polypyrimidine Tract-Binding Protein: A RNA-binding protein that binds to polypyriminidine rich regions in the INTRONS of messenger RNAs. Polypyrimidine tract-binding protein may be involved in regulating the ALTERNATIVE SPLICING of mRNAs since its presence on an intronic RNA region that is upstream of an EXON inhibits the splicing of the exon into the final mRNA product.Hu Paraneoplastic Encephalomyelitis Antigens: A family of RNA-binding proteins that are homologues of ELAV protein, Drosophila. They were initially identified in humans as the targets of autoantibodies in patients with PARANEOPLASTIC ENCEPHALOMYELITIS. They are thought to regulate GENE EXPRESSION at the post-transcriptional level.Heterogeneous-Nuclear Ribonucleoproteins: A family of ribonucleoproteins that were originally found as proteins bound to nascent RNA transcripts in the form of ribonucleoprotein particles. Although considered ribonucleoproteins they are primarily classified by their protein component. They are involved in a variety of processes such as packaging of RNA and RNA TRANSPORT within the nucleus. A subset of heterogeneous-nuclear ribonucleoproteins are involved in additional functions such as nucleocytoplasmic transport (ACTIVE TRANSPORT, CELL NUCLEUS) of RNA and mRNA stability in the CYTOPLASM.MicroRNAs: Small double-stranded, non-protein coding RNAs, 21-25 nucleotides in length generated from single-stranded microRNA gene transcripts by the same RIBONUCLEASE III, Dicer, that produces small interfering RNAs (RNA, SMALL INTERFERING). They become part of the RNA-INDUCED SILENCING COMPLEX and repress the translation (TRANSLATION, GENETIC) of target RNA by binding to homologous 3'UTR region as an imperfect match. The small temporal RNAs (stRNAs), let-7 and lin-4, from C. elegans, are the first 2 miRNAs discovered, and are from a class of miRNAs involved in developmental timing.Ribonucleoproteins: Complexes of RNA-binding proteins with ribonucleic acids (RNA).Mesenchymal Stromal Cells: Bone-marrow-derived, non-hematopoietic cells that support HEMATOPOETIC STEM CELLS. They have also been isolated from other organs and tissues such as UMBILICAL CORD BLOOD, umbilical vein subendothelium, and WHARTON JELLY. These cells are considered to be a source of multipotent stem cells because they include subpopulations of mesenchymal stem cells.Mesenchymal Stem Cell Transplantation: Transfer of MESENCHYMAL STEM CELLS between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS).Bone Marrow Cells: Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.Odontogenic Tumors: Neoplasms produced from tooth-forming tissues.Odontogenesis: The process of TOOTH formation. It is divided into several stages including: the dental lamina stage, the bud stage, the cap stage, and the bell stage. Odontogenesis includes the production of tooth enamel (AMELOGENESIS), dentin (DENTINOGENESIS), and dental cementum (CEMENTOGENESIS).Odontogenic Cysts: Cysts found in the jaws and arising from epithelium involved in tooth formation. They include follicular cysts (e.g., primordial cyst, dentigerous cyst, multilocular cyst), lateral periodontal cysts, and radicular cysts. They may become keratinized (odontogenic keratocysts). Follicular cysts may give rise to ameloblastomas and, in rare cases, undergo malignant transformation.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Nuclear Respiratory Factor 1: A transcription factor that controls the expression of variety of proteins including CYTOCHROME C and 5-AMINOLEVULINATE SYNTHETASE. It plays an important role in maintenance of the RESPIRATORY CHAIN of MITOCHONDRIA.Nuclear Respiratory Factors: A family of transcription factors that control expression of a variety of nuclear GENES encoding proteins that function in the RESPIRATORY CHAIN of the MITOCHONDRIA.NF-E2-Related Factor 1: A basic-leucine zipper transcription factor that is involved in regulating inflammatory responses, MORPHOGENESIS, and HEME biosynthesis.p300-CBP Transcription Factors: A family of histone acetyltransferases that is structurally-related to CREB-BINDING PROTEIN and to E1A-ASSOCIATED P300 PROTEIN. They function as transcriptional coactivators by bridging between DNA-binding TRANSCRIPTION FACTORS and the basal transcription machinery. They also modify transcription factors and CHROMATIN through ACETYLATION.Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.Diacylglycerol Kinase: An enzyme of the transferase class that uses ATP to catalyze the phosphorylation of diacylglycerol to a phosphatidate. EC 2.7.1.107.Sputum: Material coughed up from the lungs and expectorated via the mouth. It contains MUCUS, cellular debris, and microorganisms. It may also contain blood or pus.Pulmonary Disease, Chronic Obstructive: A disease of chronic diffuse irreversible airflow obstruction. Subcategories of COPD include CHRONIC BRONCHITIS and PULMONARY EMPHYSEMA.Bronchoalveolar Lavage Fluid: Washing liquid obtained from irrigation of the lung, including the BRONCHI and the PULMONARY ALVEOLI. It is generally used to assess biochemical, inflammatory, or infection status of the lung.Dose-Response Relationship, Drug: The relationship between the dose of an administered drug and the response of the organism to the drug.Lung Diseases, Obstructive: Any disorder marked by obstruction of conducting airways of the lung. AIRWAY OBSTRUCTION may be acute, chronic, intermittent, or persistent.Smoking: Inhaling and exhaling the smoke of burning TOBACCO.Colitis, Ulcerative: Inflammation of the COLON that is predominantly confined to the MUCOSA. Its major symptoms include DIARRHEA, rectal BLEEDING, the passage of MUCUS, and ABDOMINAL PAIN.Intestinal Mucosa: Lining of the INTESTINES, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. In the SMALL INTESTINE, the mucosa is characterized by a series of folds and abundance of absorptive cells (ENTEROCYTES) with MICROVILLI.Lymphoma, B-Cell, Marginal Zone: Extranodal lymphoma of lymphoid tissue associated with mucosa that is in contact with exogenous antigens. Many of the sites of these lymphomas, such as the stomach, salivary gland, and thyroid, are normally devoid of lymphoid tissue. They acquire mucosa-associated lymphoid tissue (MALT) type as a result of an immunologically mediated disorder.Inflammation: A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function.Remission Induction: Therapeutic act or process that initiates a response to a complete or partial remission level.Crohn Disease: A chronic transmural inflammation that may involve any part of the DIGESTIVE TRACT from MOUTH to ANUS, mostly found in the ILEUM, the CECUM, and the COLON. In Crohn disease, the inflammation, extending through the intestinal wall from the MUCOSA to the serosa, is characteristically asymmetric and segmental. Epithelioid GRANULOMAS may be seen in some patients.Colitis: Inflammation of the COLON section of the large intestine (INTESTINE, LARGE), usually with symptoms such as DIARRHEA (often with blood and mucus), ABDOMINAL PAIN, and FEVER.

Hypertrophic stimuli augment expression of cMG1/ERF-1, a putative zinc-finger motif transcription factor, in rat cardiomyocytes. (1/13)

We isolated the gene for cMG1/ERF-1, a known putative zinc-finger transcription factor, by differential display of mRNA extracted from cardiomyocytes with and without leukemia inhibitory factor (LIF) stimulation. LIF induced cMG1/ERF-1 mRNA at 15 min, and levels peaked at 10-fold initial levels at 30 min. cMG1/ERF-1 expression was inhibited by AG490 (JAK2 inhibitor) and genistein, but was unaffected by PD98059 or wortmannin. Phenylephrine, angiotensin II and endothelin-1 also induced cMG1/ERF-1 expression. Mechanical stretch in vitro and acute pressure overload in vivo increased cMG1/ERF-1 expression. To our knowledge, this is the first report showing that the cMG1/ERF-1 gene can be induced by various hypertrophic stimuli, and that Janus kinase 2 is involved in this process.  (+info)

Anti-CD20- and B-cell receptor-mediated apoptosis: evidence for shared intracellular signaling pathways. (2/13)

Clinical administration of the anti-CD20 antibody IDEC-C2B8 can induce remission of low-grade B-cell lymphoma. Whereas it has been suggested that the main mechanisms of action are complement-mediated and antibody-dependent cell-mediated cytotoxicity, we demonstrate that monoclonal antibody IDEC-C2B8 is a strong inducer of apoptosis in CD20-positive B-cell lymphoma cell lines reflecting different stages of lymphomagenesis. Thus, CD20-dependent apoptosis was inducible in human surface IgM-positive Burkitt's lymphoma cell lines as well as in more mature surface IgM-negative B-cell lymphoma cell lines carrying the t(14;18) translocation. Furthermore, in Burkitt's lymphoma cell lines, we observed a striking correlation between anti-CD20- and B-cell receptor-mediated apoptosis with regard to sensitivity toward the apoptotic stimuli and the execution of the apoptotic pathway. Thus, induction of anti-CD20- or B-cell receptor-mediated apoptosis involved rapid up-regulation of the proapoptotic protein Bax. In addition, we show similar changes in the mRNA expression level of two early response genes, c-myc and Berg36, as well as activation of the mitogen-activated protein kinase family members p44 (extracellular signal-regulated kinase 1) and p42 (extracellular signal-regulated kinase 2) and activation of activator protein 1 (AP-1) DNA binding activity. These data support our hypothesis that both pathways are mediated in part by the same signal-transducing molecules. These results might help explain the resistance and regression of lymphomas to IDEC-C2B8 and give new insights in the signaling cascade after CD20 ligation.  (+info)

Functional cloning of BRF1, a regulator of ARE-dependent mRNA turnover. (3/13)

To identify regulators of AU-rich element (ARE)-dependent mRNA turnover we have followed a genetic approach using a mutagenized cell line (slowC) that fails to degrade cytokine mRNA. Accordingly, a GFP reporter construct whose mRNA is under control of the ARE from interleukin-3 gives an increased fluorescence signal in slowC. Here we describe rescue of slowC by a retroviral cDNA library. Flow cytometry allowed us to isolate revertants with reconstituted rapid mRNA decay. The cDNA was identified as butyrate response factor-1 (BRF1), encoding a zinc finger protein homologous to tristetraprolin. Mutant slowC carries frame-shift mutations in both BRF1 alleles, whereas slowB with intermediate decay kinetics is heterozygous. By use of small interfering (si)RNA, independent evidence for an active role of BRF1 in mRNA degradation was obtained. In transiently transfected NIH 3T3 cells, BRF1 accelerated mRNA decay and antagonized the stabilizing effect of PI3-kinase, while mutation of the zinc fingers abolished both function and ARE-binding activity. This approach, which identified BRF1 as an essential regulator of ARE-dependent mRNA decay, should also be applicable to other cis-elements of mRNA turnover.  (+info)

Roles of AUF1 isoforms, HuR and BRF1 in ARE-dependent mRNA turnover studied by RNA interference. (4/13)

HT1080 cells stably expressing green fluorescent protein (GFP) linked to a 3' terminal AU-rich element (ARE) proved to be a convenient system to study the dynamics of mRNA stability, as changes in mRNA levels are reflected in increased or decreased fluorescence intensity. This study examined whether mRNA stability can be regulated by small interfering RNAs (siRNAs) targeted to AU-binding proteins (AUBPs), which in turn should reveal their intrinsic role as stabilizers or destabilizers of ARE-mRNAs. Indeed, siRNAs targeting HuR or BRF1 decreased or increased fluorescence, respectively. This effect was abolished if cells were treated with both siRNAs, thus indicating antagonistic control of ARE-mRNA stability. Unexpectedly, downregulation of all four AUF1 isoforms by targeting common exons did not affect fluorescence whereas selective downregulation of p40AUF1/p45AUF1 strongly increased fluorescence by stabilizing the GFP-ARE reporter mRNA. This observation was fully confirmed by the finding that only selective reduction of p40AUF1/p45AUF1 induced the production of GM-CSF, an endogenous target of AUF1. These data suggest that the relative levels of individual isoforms, rather than the absolute amount of AUF1, determine the net mRNA stability of ARE-containing transcripts, consistent with the differing ARE-binding capacities of the isoforms.  (+info)

Recruitment and activation of mRNA decay enzymes by two ARE-mediated decay activation domains in the proteins TTP and BRF-1. (5/13)

In human cells, a critical pathway in gene regulation subjects mRNAs with AU-rich elements (AREs) to rapid decay by a poorly understood process. AREs have been shown to directly activate deadenylation, decapping, or 3'-to-5' exonucleolytic decay. We demonstrate that enzymes involved in all three of these mRNA decay processes, as well as 5'-to-3' exonucleolytic decay, associate with the protein tristetraprolin (TTP) and its homolog BRF-1, which bind AREs and activate mRNA decay. TTP and BRF-1 each contain two activation domains that can activate mRNA decay after fusion to a heterologous RNA-binding protein, and inhibit ARE-mediated mRNA decay when overexpressed. Both activation domains employ trans-acting factors to trigger mRNA decay, and the N-terminal activation domain functions as a binding platform for mRNA decay enzymes. Our data suggest that the TTP protein family functions as a molecular link between ARE-containing mRNAs and the mRNA decay machinery by recruitment of mRNA decay enzymes, and help explain how deadenylation, decapping, and exonucleolytic decay can all be independently activated on ARE-containing mRNAs. This describes a potentially regulated step in activation of mRNA decay.  (+info)

Mitogen-induced expression of the primary response gene cMG1 in a rat intestinal epithelial cell-line (RIE-1). (6/13)

cMG1 is a primary response gene first identified in a rat intestinal epithelial (RIE-1) cell-line [(1990) Oncogene 5, 1081-1083]. A number of mitogens, including epidermal growth factor (EGF), angiotensin II (AII), serum and insulin rapidly induced 2- to 6-fold increases of cMG1 mRNA in RIE-1 cells, while transforming growth factor-beta caused a small reduction. Cyclic AMP-elevating agents blocked the increase of cMG1 mRNA induced by EGF. The AII-stimulated increase of cMG1 mRNA was blocked by the depletion of protein kinase C, whereas the EGF-stimulated increase was not affected, indicating that protein kinase C-dependent and -independent signalling pathways stimulate cMG1 expression.  (+info)

Von Hippel-Lindau gene product modulates TIS11B expression in renal cell carcinoma: impact on vascular endothelial growth factor expression in hypoxia. (7/13)

 (+info)

ZFP36L1 negatively regulates erythroid differentiation of CD34+ hematopoietic stem cells by interfering with the Stat5b pathway. (8/13)

 (+info)

*ZFP36L1

Butyrate response factor 1 is a protein that in humans is encoded by the ZFP36L1 gene. This gene is a member of the TIS11 ... Reppe S, Olstad OK, Rian E, Gautvik VT, Gautvik KM, Jemtland R (November 2004). "Butyrate response factor 1 is regulated by ... The gene is well conserved across species and has a promoter that contains motifs seen in other early-response genes. The ... This RNA binding protein most likely functions in regulating the response to growth factors. ZFP36L1 has been shown to interact ...

*Butyrate

... inhibits angiogenesis by inactivating Sp1 transcription factor activity and downregulating vascular endothelial growth ... While transient IFN-γ signaling is generally associated with normal host immune response, chronic IFN-γ signaling is often ... Examples include Cellulose acetate butyrate, an aircraft dope Methyl butyrate Ethyl butyrate Butyl butyrate Pentyl butyrate ... which leads to butyrate not being properly metabolized. This phenomenon leads to the accumulation of butyrate in the nucleus, ...

*THP-1 cell line

They can restore the response of purified T lymphocytes to Con A. They show increased CO2 production on phagocytosis and can be ... recombinant human tumour necrotic factor α (rhTNF-α) and Ionomycin. The HLA type for THP-1 using molecular-based typing methods ... They stain positive for alpha-naphthyl butyrate esterase, produce lysozymes and are phagocytic (both for latex beads and ... and recombinant human granulocyte macrophage colony-stimulating factor (rhGM-CSF), and mature dendritic cells using rhIL-4, ...

*Sirtuin 1

"Human CD34+ hematopoietic progenitor cells hyperacetylate core histones in response to sodium butyrate, but not trichostatin A ... Lawrence T (December 2009). "The nuclear factor NF-kappaB pathway in inflammation". Cold Spring Harbor perspectives in biology ... Sirtuins are hypothesized to play a key role in an organism's response to stresses (such as heat or starvation) and to be ... This function provides a link between sirtuin expression and the cellular response to limited nutrients due to caloric ...

*ZFP36

It is a member of the TIS11 (TPA-induced sequence) family, along with butyrate response factors 1 and 2. TTP binds to AU-rich ... Carballo E, Lai WS, Blackshear PJ (August 1998). "Feedback inhibition of macrophage tumor necrosis factor-alpha production by ... "A growth factor-inducible nuclear protein with a novel cysteine/histidine repetitive sequence". J Biol Chem. 265 (31): 19185-91 ... a potential zinc finger transcription factor, by mitogen stimulation in intact cells and by mitogen-activated protein kinase in ...

*Acute megakaryoblastic leukemia

Another related gene is MKL1, which is also known as "MAL". This gene is a cofactor of serum response factor. In adults, ... Megakaryoblasts lack myeloperoxidase (MPO) activity and stain negatively with Sudan black B. They are alpha naphthyl butyrate ... poor response to chemotherapy, and short clinical course. In children, the same clinical presentation but with variable course ... Huret JL . t(1;22)(p13;q13) Archived July 3, 2008, at the Wayback Machine.. Atlas Genet Cytogenet Oncol Haematol. Cuneo A, ...

*Index of oncology articles

... tumor necrosis factor - tumor suppressor gene - tumor-derived - tumor-specific antigen - TUR - TURP - TVS - tympanites - Type I ... objective response - oblimersen - obtundation - occult stage non-small cell lung cancer - octreotide - ocular melanoma - ... arginine butyrate - aromatase inhibitor - arsenic trioxide - arteriogram - arteriography - asbestos - ascites - asparaginase - ... human epidermal growth factor receptor 2 - human leukocyte antigen - human lymphocyte antigen - human papillomavirus - human T- ...

*FOSB

... response element binding protein (CREB), and nuclear factor kappa B (NF-κB). ΔFosB is the most significant biomolecular ... Most of the animal research with HDAC inhibitors has been conducted with four drugs: butyrate salts (mainly sodium butyrate), ... The transcription factor delta FosB is increased in the NAc, PFC, dorsal striatum, and VTA following repeated sexual behavior ( ... ΔJunD, a transcription factor, and G9a, a histone methyltransferase, both oppose the function of ΔFosB and inhibit increases in ...

*Moraxella catarrhalis

Many laboratories also perform a butyrate esterase test and a beta-lactamase test. Both tests should be positive and can help ... Also, a bactericidal serum antibody has also been developed in response to the diseases caused by M. catarrhalis. Treatment ... Lipooligosaccharide is considered one possible virulence factor. Since the recent recognition of M. catarrhalis as an important ... a sort of subclass-specific IgG antibody response to certain outer membrane proteins may also exist. Therefore, the outer ...

*Epigenetic therapy

Epigenetic markings can be inherited in some cases, and can change in response to environmental stimuli over the course of an ... However, brain-derived neurotrophic factor (BDNF) and the N-methyl-D-aspartate receptors (NMDA) have been identified as crucial ... sodium butyrate, and VPA, all known histone deacetylase inhibitors. Several studies in the past two years have shown that in ... and is therefore influential in the stress response in mammals, has been correlated with hypertension. Decreased LINE-1 ...

*PDK4

One metabolite, butyrate, induces hyperacetylation of the histones around the PDK4 gene. This is associated with a greater ... "PDK4 protein promotes tumorigenesis through activation of cAMP-response element-binding protein (CREB)-Ras homolog enriched in ... family is tightly regulated itself by a myriad of factors including transcription factors Sp1 and CCAAT box binding factor (CBF ... "Butyrate elicits a metabolic switch in human colon cancer cells by targeting the pyruvate dehydrogenase complex". International ...

*Corticosteroid

... including stress response, immune response, and regulation of inflammation, carbohydrate metabolism, protein catabolism, blood ... Alclometasone dipropionate, betamethasone dipropionate, betamethasone valerate, clobetasol propionate, clobetasone butyrate, ... transcription factor T-bet). Both genes display some degree of polymorphic variation in humans, which may explain how some ... Patients' response to inhaled corticosteroids has some basis in genetic variations. Two genes of interest are CHRH1 ( ...

*Gastrointestinal tract

An autoimmune response is triggered in intestinal cells by digestion of gluten proteins. Ingestion of proteins found in wheat, ... Basically, the butyrate induces the differentiation of Treg cells by enhancing histone H3 acetylation in the promoter and ... and gastric ATPase ATP4A and gastric intrinsic factor GIF, expressed in parietal cells of the stomach mucosa. Specific proteins ... This inflammatory response triggers a cascade of mucosal changes that can persist from chronic gastritis, duodenal cancer, ...

*Butyrate kinase

One study specifically identified the transcription factor NF-kB as a target of butyrate to decrease the number of pro- ... "Butyrate inhibits inflammatory responses through NFkappaB inhibition: implications for Crohn's disease". Gut. 47 (3): 397-403. ... Butyryl-phosphate is then converted into butyrate by using butyrate kinase and in the process, releases ATP. Butyrate plays an ... Butyrate kinase is active within the human colon. To form butyrate, two molecules of acetyl-CoA are combined and reduced to ...

*Ulcerative colitis

Inadequate butyrate levels in the lower intestine have been suggested as a contributing factor for the disease. This might be ... The response of ulcerative colitis is potentially very favorable with one study reporting 67.7% of sufferers experiencing ... The epithelial cells in the colon uses butyrate from the contents of the intestine as an energy source. The amount of butyrate ... Geerling BJ, Dagnelie PC, Badart-Smook A, Russel MG, Stockbrügger RW, Brummer RJ (April 2000). "Diet as a risk factor for the ...

*Induced stem cells

... transforming growth factor-β (TGF-β), basic fibroblast growth factor (bFGF), and leukemia inhibitory factor (LIF)). The ... Astrocytes-the most common neuroglial brain cells, which contribute to scar formation in response to injury-can be directly ... sodium butyrate (NaB) or Trichostatin A (TSA) could replace VPA, Lithium chloride (LiCl) or lithium carbonate (Li2CO3) could ... However, brief expression of a single transcription factor, the ELT-7 GATA factor, can convert the identity of fully ...

*Alkaline phosphatase

A study based on sodium butyrate effects on cancer cells conveys that it has an effect on androgen receptor co-regulator ... Higher levels are seen in the physiological response, the leukemoid reaction, and in pathologies that include mature white ... Current researchers are looking into the increase of tumor necrosis factor-α and its direct effect on the expression of ... This explains why the addition of sodium butyrate show increased activity of alkaline phosphatase in the cancer cells of the ...

*Epigenetics of neurodegenerative diseases

Sodium butyrate Sodium butyrate is a class I and II HDACi and has been shown to recover learning and memory after 4 weeks ( ... a neurotrophic factor important for long-term memory. Expression of CREB, an activity-dependent transcription factor involved ... as well as with immune response. Hypomethylation has been observed in promoters of presenilin 1 (Wang et al., 2008), GSK3beta, ... Sodium Butyrate Sodium butyrate was the first HDAC inhibitor tested in SMA mouse models. It prolonged SMA mouse life span by 35 ...

*Histone deacetylase inhibitor

HDAC1 negatively regulates the cardiovascular transcription factor Kruppel-like factor 5 through direct interaction. Estrogen ... Sodium butyrate is commonly used as a candidate for mood disorder treatment: studies using it both alone and in co-treatment ... Recent studies [...] have shown that the estrogen receptor alpha (ERalpha) can be hyperacetylated in response to histone ... "The deacetylase HDAC1 negatively regulates the cardiovascular transcription factor Krüppel-like factor 5 through direct ...

*Cingulin

Kos R, Reedy MV, Johnson RL, Erickson CA (April 2001). "The winged-helix transcription factor FoxD3 is important for ... Furthermore, histone deacetylase inhibitors, such as sodium butyrate, strongly upregulate its expression in some cultured cells ... and plays a role in the control of claudin-2 expression and response to duodenal mucosa injury". Journal of Cell Science. 125 ( ... 7 (1): 65-73. doi:10.1093/dnares/7.1.65. PMID 10718198. "Entrez Gene: CGN cingulin". Citi S, Sabanay H, Jakes R, Geiger B, ...

*Vespula pensylvanica

... heptyl butyrate, and butyl butyrate. Acetic acid alone did not have the same attractive effects as the chemical combinations. ... Generally, climate is a constraining factor in the reproductive behavior of V. pensylvanica. When temperatures drop, food ... "Yellowjackets (Vespula Pensylvanica) Thermoregulate in Response to Changes in Protein Concentration." Naturwissenschaften 95.9 ... Using a vial dispenser, releasing up to an estimated 2.3 mg of heptyl butyrate per hour increased the number of yellowjackets ...

*Methamphetamine

ΔJunD, a transcription factor, and G9a, a histone methyltransferase enzyme, both directly oppose the induction of ΔFosB in the ... This is known to occur on many genes including fosB and c-fos in response to psychostimulant exposure. ΔFosB is also ... "Substrate/Product". butyrate-CoA ligase. BRENDA. Technische Universität Braunschweig. Retrieved 7 May 2014. "Substrate/Product ... Chronic exposure to amphetamine induces a unique transcription factor delta FosB, which plays an essential role in long-term ...

*Adderall

... scaling factor = (molecular masstotal / molecular massbase) × scaling factor. The values in this column were scaled to a 30 mg ... Zhou Y, Zhao M, Zhou C, Li R (July 2015). "Sex differences in drug addiction and response to exercise intervention: From human ... CYP2D6, dopamine β-hydroxylase (DBH), flavin-containing monooxygenase 3 (FMO3), butyrate-CoA ligase (XM-ligase), and glycine N- ... ΔJunD, a transcription factor, and G9a, a histone methyltransferase enzyme, both oppose the function of ΔFosB and inhibit ...

*Epigenetics of cocaine addiction

ΔFosB is a cocaine-induced transcription factor that accumulates in the nucleus accumbens (NAc) and acts to repress G9a. When ... They found unexpected patterns of brain activation in awake rats that were exposed to sodium butyrate, an HDAC inhibitor (or ... The NK1R gene promoter has been associated with enhanced response to cocaine reward, meaning HDAC5 in normal genomes may ... Sodium butyrate co-treatment with cocaine restored pronounced BOLD activation after successive cocaine treatments. These ...

*Dietary fiber

The major SCFAs in humans are butyrate, propionate, and acetate, where butyrate is the major energy source for colonocytes, ... A further factor is an increase in the bacterial mass and activity of the ileum as some fibers e.g., pectin are digested by ... Viscous fibers thicken the contents of the intestinal tract and may attenuate the absorption of sugar, reduce sugar response ... In the preferred food choices of today's youth, this value may be as low as 20%, a factor considered by experts as contributing ...

*Food intolerance

... and gastro-intestinal responses to foods or food compounds. Food intolerance does not include either psychological responses or ... Hamer HM, Jonkers D, Venema K, Vanhoutvin S, Troost FJ, Brummer RJ (January 2008). "Review article: the role of butyrate on ... Patients consider food intolerance and GPs regard lack of fibre as the main etiologic dietary factor. It has been suggested ... Immunological responses are mediated by non-IgE immunoglobulins, where the immune system recognises a particular food as a ...
The histone lysine methyltransferase nuclear receptor-binding SET domain protein 2 (NSD2, also known as WHSC1/MMSET) is an epigenetic modifier and thought to play a driving role in oncogenesis. Both NSD2 overexpression and point mutations that increase its catalytic activity are associated with several human cancers. While NSD2 is an attractive therapeutic target, no potent, selective, and bioactive small molecule inhibitors of NSD2 have been reported to date, possibly due to the challenges of developing high-throughput assays for NSD2. Here, to establish a platform for the discovery and development of selective NSD2 inhibitors, we optimized and implemented multiple assays. We performed quantitative high-throughput screening with full-length wild-type NSD2 and a nucleosome substrate against a diverse collection of bioactive small molecules comprising 16,251 compounds. We further interrogated 174 inhibitory compounds identified in the primary screen with orthogonal and counter assays and with ...
May play a role in subcellular trafficking between the endoplasmic reticulum and Golgi apparatus through interactions with the Rho-type GTPases. Binding to the NS3 protein of dengue virus type 2 appears to subvert this activity into the alteration of the intracellular membrane structure associated with flaviviral replication.
Risk Profile: Moderate Growth The CMG Tactical Rotation Strategy seeks to generate returns in all market conditions based on the concept that various
Regulation of mRNA turnover is an important process in determining levels of gene expression. mRNA stability varies considerably from one mRNA species to another and is determined by specific cis-acting elements within the mRNA molecule. mRNAs encoding cytokines and proto-oncogenes are degraded rapidly in order to minimize potentially inflammatory or oncogenic effects that may result from their overexpression. Many of these transcripts contain cis-acting instability elements within their 3 untranslated regions that are believed to activate mRNA decay pathways. The AU-rich elements (AREs) appear to be prominent elements that direct rapid mRNA decay by a process referred to as ARE-mediated mRNA decay (AMD). AMD is regulated by RNA-binding proteins. A number of proteins have been described to bind AREs and are collectively called ARE-binding proteins (ARE-BPs). Decay-promoting ARE-BPs bind ARE-containing mRNAs and target them for decay by recruitment of mRNA decay enzymes. Our research is directed ...
This gene encodes a protein containing a SET domain, 2 LXXLL motifs, 3 nuclear translocation signals (NLSs), 4 plant homeodomain (PHD) finger regions, and a proline-rich region. The encoded protein enhances androgen receptor (AR) transactivation, and this enhancement can be increased further in the presence of other androgen receptor associated coregulators. This protein may act as a nucleus-localized, basic transcriptional factor and also as a bifunctional transcriptional regulator. Mutations of this gene have been associated with Sotos syndrome and Weaver syndrome. One version of childhood acute myeloid leukemia is the result of a cryptic translocation with the breakpoints occurring within nuclear receptor-binding Su-var, enhancer of zeste, and trithorax domain protein 1 on chromosome 5 and nucleoporin, 98-kd on chromosome 11. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008 ...
Chinas demand for phenol has grown at a fast pace in the past decade. In the next five years, both production and demand will continue to grow. This new study examines Chinas economic trends, investment environment, industry development, supply and demand, industry capacity, industry structure, marketing channels and major industry participants. Historical data (2002, 2007 and 2012) and long-term forecasts through 2017 and 2022 are presented. Major producers in China are profiled ...
RecName: Full=A-kinase anchor protein 13; Short=AKAP-13;AltName: Full=Protein kinase A-anchoring protein 13;AltName: Full=Breast cancer nuclear receptor-binding auxiliary protein;AltName: Full=Human thyroid-anchoring protein 31;AltName: Full=Guanine nucleotide exchange factor Lbc;AltName: Full=AKAP-Lbc;AltName: Full=P47;AltName: Full=Lymphoid blast crisis oncogene; Short=LBC oncogene;AltName: Full=Non-oncogenic Rho GTPase-specific GTP exchange ...
... B-cell receptor-mediated signaling machinery. the PLSR response adjustable within the C cell people is normally both a quality tag of healthful examples and predictive of disease aggressiveness. Bottom line Single-cell multidimensional evaluation of BCR signaling allowed concentrated evaluation of the variability and heterogeneity of signaling behavior from patient-to-patient, and from cell-to-cell. Interruption of the pSYK/pPLC2 romantic relationship is normally open as a sturdy trademark of CLL C cell signaling behavior. Jointly, these findings implicate story components of the BCR indication transduction as potential healing goals. Launch Chronic lymphocytic leukemia (CLL) outcomes from the deposition of older monoclonal Compact disc5+ C cells in the bone fragments marrow, lymphoid areas and peripheral bloodstream. CLL C cells are characterized by low appearance of surface area Compact disc20 and co-expression of ...
RSV and hMPV infection modulates ARE-dependent gene transcription(A) A549 cells were transiently transfected with a plasmid containing multiple copies of the NQ
Czech myeloma group represents the association of doctors, specialists and other research workers whose aim is to examine and diagnose a disease called multiple myeloma. The associations broad objective is the diagnostics of diseases belonging to a group called monoclonal gamapathy. CMG organizes educational, experimental and other collective activities.
Czech myeloma group represents the association of doctors, specialists and other research workers whose aim is to examine and diagnose a disease called multiple myeloma. The associations broad objective is the diagnostics of diseases belonging to a group called monoclonal gamapathy. CMG organizes educational, experimental and other collective activities.
InfoBank, KnowledgeBanks field data catalog, is a structured information storage scheme of databases and software that provide organized access to USGS Coastal and Marine data and metadata.
InfoBank, KnowledgeBanks field data catalog, is a structured information storage scheme of databases and software that provide organized access to USGS Coastal and Marine data and metadata.
is it possible to optimize a transfected cell-line (by G418-selection) by subcloning, i. e. to get a better receptor expession. I think if i got a monoclonal mammalial cell-line which is overexpressing a receptor gene, it could improve the expression of this gene, to make a subcloning by dilution. I think the cells are dividing over the time and are so not longer be monoclonal ...
KMT3B / NSD1兔多克隆抗体(ab84137)可与人样本反应并经IHC实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
The Positive Transcription Elongation Factor b (P-TEFb) is a complex of Cyclin Dependent Kinase 9 (CDK9) with either cyclins T1, T2 or K. The complex phosphorylates the C-Terminal Domain of RNA polymerase II (RNAPII) and negative elongation factors, stimulating productive elongation by RNAPII, which is paused after initiation. P-TEFb is recruited downstream of the promoters of many genes, including primary response genes, upon certain stimuli. Flavopiridol (FVP) is a potent pharmacological inhibitor of CDK9 and has been used extensively in cells as a means to inhibit CDK9 activity. Inhibition of P-TEFb complexes has potential therapeutic applications. It has been shown that Lipopolysaccharide (LPS) stimulates the recruitment of P-TEFb to Primary Response Genes (PRGs) and proposed that P-TEFb activity is required for their expression, as the CDK9 inhibitor DRB prevents localization of RNAPII in the body of these genes. We have previously determined the effects of FVP in global gene expression in a
There is an industry need for new high performance technologies that achieve the goal of meeting the unmet needs of bio-manufacturing. These technologies must be: effective, safe, have decent capacity, rapid response, be cost-effective and simple to manufacture. Fraunhofer USA are attempting to produce these technologies. Pilot production has operated to USFDA cGMP standards. Should the technologies be biological- can the system produce proteins with desired characteristics? Should the technologies be commercial- do they attributes that make the technology competitive? Should the technologies be regulatory- are they safe, potent and effective? Tell us your opinion by commenting in the space provided below! Dr Vidadi Yusibov, Executive Director, Fraunhofer USA joined us at the World Vaccine Congress 2012 in Washington to discuss whether or not one cell-line can meet the demands of product quantities and varieties. Why not download the full presentation to find out more about: ...
TY - JOUR. T1 - Phthalate plasticizer di(2-ethyl-hexyl) phthalate induces cyclooxygenase-2 expression in gastric adenocarcinoma cells. AU - Wong, Jhen Hong. AU - Wang, Yu Shiuan. AU - Nam, Sean. AU - Ho, Kuo Hao. AU - Chang, Che Mai. AU - Chen, Ku Chung. AU - Chen, Yi Fan. AU - Chang, Wei Chiao. PY - 2019/1/1. Y1 - 2019/1/1. N2 - The phthalate plasticizer, di(2-ethyl-hexyl) phthalate (DEHP), and its derived metabolites are common anthropogenic environmental toxins, which are known to act as endocrine disruptors. Numerous studies have associated DEHP with disruption of sex hormones, abnormal development of reproductive organs, allergies, and inflammation. Its role in promoting inflammation has been reported by both human epidemiological and animal studies. In stomach tissue, chronic inflammation is known to accompany mucosal damage, and pave the way to gastritis, stomach ulcers, and ultimately gastric cancer. Eastern Asian populations possess the highest gastric cancer incidences in the world. ...
E4orf6 exports and stabilizes ARE-mRNAs. (A) HuR, which bound to mRNA, was isolated from the nuclear (N) and cytoplasmic (C) fractions of each BRK cell exposed to UV light by oligo (dT)-cellulose chromatography (left). The amount of HuR in total extract (TE) of each cell is shown (middle). The fractions and TE were analyzed by immunoblotting with antibodies to β-tubulin and poly(ADP-ribose)polymerase (PARP; right). (B) The amount of c-fos, c-myc, COX-2, and GAPDH mRNA expressed in the cytoplasm of each cell was measured by quantitative real-time RT-PCR. BRK E1 and E1+E4 cells (left), 293 cells transfected with E4orf6 expression vector (middle), and HeLa cells infected with Ad dl309 and Ad dl355 (right) were used. (C) pCMVGL-ARE, which has ARE of c-fos in 3′-UTR of luciferase cDNA (left), were transfected into 293 cells with or without E4orf6 expression construct, and then the accumulation of the cytoplasmic luciferase mRNA was analyzed by Northern blot 24 h after the transfection. The ...
Transforming growth factor β1 (TGF-β1) is a potent growth inhibitor for many cell types, including tumor cells. We recently have reported the establishment and characterization of two human gastric scirrhous carcinoma cell lines, HSC-39 and HSC-43. Here we examined the effect of TGF-β1 on the growth of these lines as compared to five other human gastric adenocarcinoma cell lines. Proliferation of HSC-39 and HSC-43 cells was strongly inhibited by TGF-β1, whereas the other gastric adenocarcinoma cell lines were unresponsive to TGF-β1. Both HSC-39 and HSC-43 cells gradually lost viability following exposure to TGF-β1. This response was dose dependent up to 4 ng/ml. When TGF-β1 was removed, the cells failed to exhibit regrowth, indicating an irreversible growth-inhibitory effect of this agent, leading to cell death. DNA fragments were observed consisting of multimers of approximately 180 base pairs 24 h after TGF-β1 treatment. The chromatin condensation of each cell line was confirmed by ...

Frontiers | ACTH Action on Messenger RNA Stability Mechanisms | EndocrinologyFrontiers | ACTH Action on Messenger RNA Stability Mechanisms | Endocrinology

This process occurs in response to modifications of the cellular environment, including hormonal variations, and regulates the ... This process occurs in response to modifications of the cellular environment, including hormonal variations, and regulates the ... TIS11b, TPA-inducible sequence 11b; BRF1, butyrate response factor 1; ZFP36, zinc finger protein; TTP, tristetraprolin; CCR4, ... through stabilization of cAMP response element-binding protein (CREB) transcription factor. J Biol Chem (2011) 286:32976-85. ...
more infohttps://www.frontiersin.org/articles/10.3389/fendo.2017.00003/full

EXOSC3 Gene - GeneCards | EXOS3 Protein | EXOS3 AntibodyEXOSC3 Gene - GeneCards | EXOS3 Protein | EXOS3 Antibody

Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA. *Deadenylation-dependent mRNA decay ... Transcription Factor. Binding Sites. Gene Targets. GH09J037799. Promoter/Enhancer. 2.1. EPDnew Ensembl ENCODE 687.8. +0.3. 308 ... Top Transcription factor binding sites by QIAGEN in the EXOSC3 gene promoter:. *AREB6 ... No data available for Enzyme Numbers (IUBMB) , miRNA , Transcription Factor Targets and HOMER Transcription for EXOSC3 Gene ...
more infohttps://www.genecards.org/cgi-bin/carddisp.pl?gene=EXOSC3

EXOSC4 Gene - GeneCards | EXOS4 Protein | EXOS4 AntibodyEXOSC4 Gene - GeneCards | EXOS4 Protein | EXOS4 Antibody

Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA. *Deadenylation-dependent mRNA decay ... Transcription Factor Binding Sites within enhancer. Gene Targets for Enhancer. GH08H143968. 1.6. Ensembl ENCODE dbSUPER 11.4. - ... DDX60, a DEXD/H box helicase, is a novel antiviral factor promoting RIG-I-like receptor-mediated signaling. (PMID: 21791617) ... Transcription factor binding sites by QIAGEN in the EXOSC4 gene promoter:. *E2F ...
more infohttp://www.genecards.org/cgi-bin/carddisp.pl?id_type=entrezgene&id=54512

CST - BRF1/2 AntibodyCST - BRF1/2 Antibody

... and butyrate response factor 2 (BRF2; also known as EGF response factor 2 [ERF2], TIS11D, ZFP36L2) both belong to the TIS11 ... Butyrate response factor 1 (BRF1; also known as EGF response factor 1 [ERF1], TIS11B, ZFP36L1) ... 10X Tris Buffered Saline with Tween® 20 (TBST): (#9997) To prepare 1 L 1X TBST: add 100 ml 10X TBST to 900 ml dH2O, mix. ... 10X Tris-Glycine SDS Running Buffer: (#4050) To prepare 1 L 1X running buffer: add 100 ml 10X running buffer to 900 ml dH2O, ...
more infohttps://www.cellsignal.com/products/primary-antibodies/brf1-2-antibody/2119

Exosc7 exosome component 7 [Mus musculus (house mouse)] - Gene - NCBIExosc7 exosome component 7 [Mus musculus (house mouse)] - Gene - NCBI

Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA, organism-specific biosystem (from REACTOME) Butyrate Response ... Transcription factor Foxp3 and its protein partners form a complex regulatory network. Rudra D, et al. Nat Immunol, 2012 Oct. ... Conserved Domains (1) summary. cd11367. Location:5 → 276. RNase_PH_RRP42; RRP42 subunit of eukaryotic exosome. ... Conserved Domains (1) summary. cd11367. Location:8 → 198. RNase_PH_RRP42; RRP42 subunit of eukaryotic exosome. ...
more infohttps://www.ncbi.nlm.nih.gov/gene/?term=66446

SKI6 - Exosome complex component SKI6 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Bakers yeast) - SKI6 gene &...SKI6 - Exosome complex component SKI6 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) - SKI6 gene &...

R-SCE-450385 Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA. R-SCE-450513 Tristetraprolin (TTP, ZFP36) binds and ... R-SCE-450385 Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA. R-SCE-450513 Tristetraprolin (TTP, ZFP36) binds and ... 3.30.230.70, 1 hit. InterProi. View protein in InterPro. IPR001247 ExoRNase_PH_dom1. IPR015847 ExoRNase_PH_dom2. IPR036345 ... P46948-1 [UniParc]FASTAAdd to basketAdded to basket. « Hide. 10 20 30 40 50. MSRLEIYSPE GLRLDGRRWN ELRRFESSIN THPHAADGSS ...
more infohttps://www.uniprot.org/uniprot/P46948

14-3-3zeta - 14-3-3 protein zeta - Drosophila melanogaster (Fruit fly) - 14-3-3zeta gene & protein14-3-3zeta - 14-3-3 protein zeta - Drosophila melanogaster (Fruit fly) - 14-3-3zeta gene & protein

R-DME-450385 Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA. R-DME-450604 KSRP (KHSRP) binds and destabilizes ... R-DME-450385 Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA. R-DME-450604 KSRP (KHSRP) binds and destabilizes ... cytoplasmic sequestering of transcription factor Source: FlyBaseInferred from mutant phenotypei*19900439 ... 1 - 248. 14-3-3 protein zetaAdd BLAST. 248. Proteomic databases. PaxDb, a database of protein abundance averages across all ...
more infohttps://www.uniprot.org/uniprot/P29310

EXOSC9 exosome component 9 [Homo sapiens (human)] - Gene - NCBIEXOSC9 exosome component 9 [Homo sapiens (human)] - Gene - NCBI

Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA, organism-specific biosystem (from REACTOME) Butyrate Response ... organism-specific biosystemButyrate Response Factor 1 (BRF1, ZFP36L1, not to be confused with transcription factor IIIB) binds ... Unfolded Protein Response (UPR), organism-specific biosystemThe Unfolded Protein Response (UPR) is a regulatory system that ... ATF4 activates genes, organism-specific biosystemATF4 is a transcription factor and activates expression of IL-8, MCP1, IGFBP-1 ...
more infohttps://www.ncbi.nlm.nih.gov/gene/5393

Reactome | YWHAB [cytosol]Reactome | YWHAB [cytosol]

Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA (Homo sapiens) * p-S54,92,203-BRF1 binds 14-3-3 (Homo sapiens) * ... 14-3-3 protein beta/alpha, 143B protein, Protein kinase C inhibitor protein-1, KCIP-1, Protein 1054 ...
more infohttps://reactome.org/content/detail/R-HSA-48888

ZFP36L1 - WikipediaZFP36L1 - Wikipedia

Butyrate response factor 1 is a protein that in humans is encoded by the ZFP36L1 gene. This gene is a member of the TIS11 ... Reppe S, Olstad OK, Rian E, Gautvik VT, Gautvik KM, Jemtland R (November 2004). "Butyrate response factor 1 is regulated by ... The gene is well conserved across species and has a promoter that contains motifs seen in other early-response genes. The ... This RNA binding protein most likely functions in regulating the response to growth factors. ZFP36L1 has been shown to interact ...
more infohttps://en.wikipedia.org/wiki/ZFP36L1

Reactome | BRF1 Complex recruits RNA degradation activitiesReactome | BRF1 Complex recruits RNA degradation activities

Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA (Canis familiaris) * BRF1 Complex recruits RNA degradation ...
more infohttp://www.reactome.org/content/detail/R-CFA-450488

Reactome | BRF1 Complex recruits RNA degradation activitiesReactome | BRF1 Complex recruits RNA degradation activities

Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA (Mus musculus) * BRF1 Complex recruits RNA degradation activities ...
more infohttp://www.reactome.org/content/detail/R-MMU-450488

Zinc finger, CCCH-type (IPR000571) | InterPro | EMBL-EBIZinc finger, CCCH-type (IPR000571) | InterPro | EMBL-EBI

... butyrate response factor 1), a probable regulatory protein involved in regulating the response to growth factors, and the mouse ... Another protein containing this domain is the human splicing factor U2AF 35kDa subunit, which plays a critical role in both ... They were first identified as a DNA-binding motif in transcription factor TFIIIA from Xenopus laevis (African clawed frog), ... Feedback inhibition of macrophage tumor necrosis factor-alpha production by tristetraprolin.. Science 281 1001-5 1998 ...
more infohttp://www.ebi.ac.uk/interpro/entry/IPR000571

Frontiers | Post-Transcriptional Control of the Hypoxic Response by RNA-Binding Proteins and MicroRNAs | Frontiers in Molecular...Frontiers | Post-Transcriptional Control of the Hypoxic Response by RNA-Binding Proteins and MicroRNAs | Frontiers in Molecular...

... we review the RBPs and miRNAs that modulate mRNA turnover and translation in response to hypoxic challenge. RBPs such as HuR ( ... we review the RBPs and miRNAs that modulate mRNA turnover and translation in response to hypoxic challenge. RBPs such as HuR ( ... iron-response element binding proteins (IRPs), and cytoplasmic polyadenylation-element-binding proteins (CPEBs), selectively ... iron-response element binding proteins (IRPs), and cytoplasmic polyadenylation-element-binding proteins (CPEBs), selectively ...
more infohttps://www.frontiersin.org/articles/10.3389/fnmol.2011.00007/full

Panoply™ Human EXOSC9 Knockdown Stable Cell Line - Creative BiogenePanoply™ Human EXOSC9 Knockdown Stable Cell Line - Creative Biogene

Destabilization of mRNA by Butyrate Response Factor 1 (BRF1), organism-specific biosystem; Destabilization of mRNA by KSRP, ...
more infohttps://www.creative-biogene.com/Panoply-Human-EXOSC9-Knockdown-Stable-Cell-Line-CSC-DC005149-1258168-14.html

iRoot BP Plus promotes osteo/odontogenic differentiation of bone marrow mesenchymal stem cells via MAPK pathways and autophagy ...iRoot BP Plus promotes osteo/odontogenic differentiation of bone marrow mesenchymal stem cells via MAPK pathways and autophagy ...

... butyrate response factor 1 regulates inflammation of RAW264.7 cells by autophagy crosstalking with the ERK pathway [52]. High ... Comparison of in vivo dental pulp responses to capping with iRoot BP Plus and mineral trioxide aggregate. Int Endod J. 2016;49: ... Recently, autophagy has been recognized to be an important factor in stem cell differentiation. Aged BMMSCs have a ... In addition to biomaterials, stem cells are another key factor in tissue engineering [11]. During regenerative endodontic ...
more infohttps://stemcellres.biomedcentral.com/articles/10.1186/s13287-019-1345-3

SMART: ZnF C3H1 domain annotationSMART: ZnF C3H1 domain annotation

... butyrate response factor 1), a probable regulatory protein involved in regulating the response to growth factors, and the mouse ... They were first identified as a DNA-binding motif in transcription factor TFIIIA from Xenopus laevis (African clawed frog), ... Another protein containing this domain is the human splicing factor U2AF 35kDa subunit, which plays a critical role in both ... Nup475 is a nuclear zinc-binding protein of unknown function that is induced in mammalian cells by growth factor mitogens. ...
more infohttp://smart.embl.de/smart/do_annotation.pl?DOMAIN=ZnF_C3H1&

biobar.ca - Catalogbiobar.ca - Catalog

B9000-97 -200ul : Butyrate Response Factor 1 (BRF1, Berg36, C3H Type-like 1, cMG1, Early Response Factor Berg36, EGF-inducible ... B8996-82 -500mg : (-)-(2Z,5S,6E)-5-{[tert-Butyl(dimethyl)silyl]oxy}-2,6-dimethyl-7-(2-methyl-1,3-thiazol-4-yl)hepta-2,6-dien-1- ... B8997-03 -5mg : O-(N-Butylfluorescein)-O-{3-[6-O-(D,L-1-ethoxyethyl)-1,2:4,5-bis-O-(1-methylethylidene)-D,L-myo-inositol]} ... B8996-88 -1g : (-)-(3S,4E)-3-{[tert-Butyl(dimethyl)silyl]oxy}-4-methyl-5-(2-methyl-1,3-thiazol-4-yl)pent-4-en-1-ol ...
more infohttps://www.biobar.ca/index.php?content=catalog&lang=en-CA&p=315

C3G | SpringerLinkC3G | SpringerLink

Guanine nucleotide-releasing factor 2 (GRF2) Small GTPases belonging to Ras... ... Crk SH3 domain binding guanine nucleotide release protein (C3G), Rap guanine nucleotide exchange factor 1 (RAPGEF1), ... Signaling of hepatocyte growth factor/scatter factor (HGF) to the small GTPase Rap1 via the large docking protein Gab1 and the ... Butyrate Response Factor 1 * BY55 * Bypass Sod1p Defects (Bsd2p) (Saccharomyces cerevisiae) * BZP ...
more infohttps://link.springer.com/referenceworkentry/10.1007%2F978-3-319-67199-4_101544

Tristetraprolin (ZFP36) and TIS11B (ZFP36-L1) | SpringerLinkTristetraprolin (ZFP36) and TIS11B (ZFP36-L1) | SpringerLink

Growth factor-inducible nuclear protein NUP475; RNF162A; TIS11; TIS11A; Tristetraprolin... ... All Names: BERG36; Berg36; BRF1; Butyrate response factor 1; cMG1; Early response factor Berg36; EGF-response factor 1; ERF-1; ... All Names: G0/G1 switch regulatory protein 24; G0S24; Growth factor-inducible nuclear protein NUP475; RNF162A; TIS11; TIS11A; ... Feedback inhibition of macrophage tumor necrosis factor-alpha production by tristetraprolin. Science. 1998;281:1001-5.PubMed ...
more infohttps://link.springer.com/referenceworkentry/10.1007%2F978-3-319-67199-4_101947

p300/CBP-associated factor (P/CAF) interacts with nuclear respiratory factor-1 to regulate the UDP-N-acetyl-alpha-d...p300/CBP-associated factor (P/CAF) interacts with nuclear respiratory factor-1 to regulate the UDP-N-acetyl-alpha-d...

... gene under the control of GalNAc-T3 indicated that several transcriptional elements are involved in response to sodium butyrate ... p300/CBP-associated factor (P/CAF) interacts with nuclear respiratory factor-1 to regulate the UDP-N-acetyl-alpha-d- ... p300/CBP-associated factor (P/CAF) interacts with nuclear respiratory factor-1 to regulate the UDP-N-acetyl-alpha-d- ... p300/CBP-associated factor (P/CAF) interacts with nuclear respiratory factor-1 to regulate the UDP-N-acetyl-alpha-d- ...
more infohttp://www.biochemj.org/content/373/3/713

Repressor Proteins | Harvard Catalyst Profiles | Harvard CatalystRepressor Proteins | Harvard Catalyst Profiles | Harvard Catalyst

Butyrate Response Factor 1. *Centromere Protein B. *Early Growth Response Transcription Factors ... Defining human cardiac transcription factor hierarchies using integrated single-cell heterogeneity analysis. Nat Commun. 2018 ... Acinetobacter baumannii OxyR Regulates the Transcriptional Response to Hydrogen Peroxide. Infect Immun. 2019 01; 87(1). ...
more infohttps://connects.catalyst.harvard.edu/Profiles/display/Concept/Repressor%20Proteins

BUB1 | Springer for Research & DevelopmentBUB1 | Springer for Research & Development

BUBR1 Bub1was originally discovered as a gene required for cell cycle arrest during mitosis in response to the microtubule... ... Butyrate Response Factor 1 * BY55 * Bypass Sod1p Defects (Bsd2p) (Saccharomyces cerevisiae) * BZP ... S. cerevisiae genes required for cell cycle arrest in response to loss of microtubule function. Cell. 1991;66:507-17.PubMed ... Bub1 was originally discovered as a gene required for cell cycle arrest during mitosis in response to the microtubule ...
more infohttps://rd.springer.com/referenceworkentry/10.1007/978-3-319-67199-4_101546

mRNA Cleavage and Polyadenylation Factors | Profiles RNSmRNA Cleavage and Polyadenylation Factors | Profiles RNS

Butyrate Response Factor 1. *Fragile X Mental Retardation Protein. *Gene Products, rev ... De Iaco A, Santoni F, Vannier A, Guipponi M, Antonarakis S, Luban J. TNPO3 protects HIV-1 replication from CPSF6-mediated ... Direct Visualization of HIV-1 Replication Intermediates Shows that Capsid and CPSF6 Modulate HIV-1 Intra-nuclear Invasion and ...
more infohttps://profiles.umassmed.edu/display/122850
  • Proteins containing CCCH Znf domains include Znf proteins from eukaryotes involved in cell cycle or growth phase-related regulation, e.g. human TIS11B (butyrate response factor 1), a probable regulatory protein involved in regulating the response to growth factors, and the mouse TTP growth factor-inducible nuclear protein, which has the same function. (ebi.ac.uk)
  • By similar analyses, XC3H-2 and XC3H-3 are homologues of ERF-1 (cMG1, TIS11B) and ERF-2 (TIS11D). (embl.de)
  • This process occurs in response to modifications of the cellular environment, including hormonal variations, and regulates the expression of subsets of proteins whose levels need to be rapidly adjusted. (frontiersin.org)
  • Through binding to its adenylate-cyclase-coupled receptor MC2R, ACTH stimulates the release of cAMP and the activation of the cAMP-dependent protein kinase A (PKA), which in turn phosphorylates and regulates a number of specific substrates including the steroidogenic acute regulatory protein (StAR) ( 1 ) and the cAMP response element-binding protein (CREB) ( 2 ). (frontiersin.org)
  • ACTH also strongly regulates the transcription of a number of genes involved in the steroidogenic response including those encoding several steroidogenic enzymes ( 3 ), components of the extracellular matrix ( 4 ) and many others. (frontiersin.org)
  • Mitra A, Kalayarasan S, Gupta V, Radha V. TC-PTP dephosphorylates the guanine nucleotide exchange factor C3G (RapGEF1) and negatively regulates differentiation of human neuroblastoma cells. (springer.com)
  • Acinetobacter baumannii OxyR Regulates the Transcriptional Response to Hydrogen Peroxide. (harvard.edu)
  • It regulates GENETIC TRANSCRIPTION involved in METABOLISM of AMINO ACIDS in response to the increased concentration of LEUCINE. (rush.edu)
  • Butyrate (also known as butanoate) is the traditional name for the conjugate base of butyric acid (also known as butanoic acid). (wikipedia.org)
  • They were first identified as a DNA-binding motif in transcription factor TFIIIA from Xenopus laevis (African clawed frog), however they are now recognised to bind DNA, RNA, protein and/or lipid substrates [ PMID: 10529348 , PMID: 15963892 , PMID: 15718139 , PMID: 17210253 , PMID: 12665246 ]. (ebi.ac.uk)
  • Another protein containing this domain is the human splicing factor U2AF 35kDa subunit, which plays a critical role in both constitutive and enhancer-dependent splicing by mediating essential protein-protein interactions and protein-RNA interactions required for 3' splice site selection. (ebi.ac.uk)
  • Ancient organisms were against IRS-1-subunit of the insulin receptor from Upstate. (hifsignaling.com)
  • RBPs such as HuR (human antigen R), PTB (polypyrimidine tract-binding protein), heterogeneous nuclear ribonucleoproteins (hnRNPs), tristetraprolin, nucleolin, iron-response element-binding proteins (IRPs), and cytoplasmic polyadenylation-element-binding proteins (CPEBs), selectively bind to numerous hypoxia-regulated transcripts and play a major role in establishing hypoxic gene expression patterns. (frontiersin.org)
  • Direct Visualization of HIV-1 Replication Intermediates Shows that Capsid and CPSF6 Modulate HIV-1 Intra-nuclear Invasion and Integration. (umassmed.edu)
  • Based on 49% overall amino acid identity and 84% amino acid identity within the double zinc finger domain, one of the Xenopus proteins (XC3H-1) appears to be the homologue of TTP. (embl.de)
  • The characterization of the tristetraprolin (TTP) family of RNA-binding proteins started in the early 1990s when Harvey Herschmann first cloned an immediate-early response gene induced by the carcinogenic phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) that he named TIS-11, standing for TPA-induced sequence 11 (Varnum et al. (springer.com)
  • J Exp Med, 1991 Apr 1. (nih.gov)
  • 1991 ). Mutant yeast were unable to arrest and inhibit the budding process at the end of mitosis, a marker for cell cycle progression, hence the name Budding Uninhibited by Benzimidazole 1 (BUB1). (springer.com)
  • Flag-tagged NRF-1 expressed in MCF-7 cells can bind to the NRF-1-binding motif of the GalNAc-T3 promoter. (biochemj.org)
  • Butyrate possesses both preventive and therapeutic potential to counteract inflammation-mediated ulcerative colitis (UC) and colorectal cancer. (wikipedia.org)
  • The reason why butyrate is an energy source for normal colonocytes and induces apoptosis in colon cancer cells, is due to the Warburg effect in cancer cells, which leads to butyrate not being properly metabolized. (wikipedia.org)
  • Microbial Degradation Products Influence Colon Cancer Risk: the Butyrate Controversy. (wikipedia.org)
  • Espeut J, Lara-Gonzalez P, Sassine M, Shiau AK, Desai A, Abrieu A. Natural loss of Mps1 kinase in nematodes uncovers a role for polo-like kinase 1 in spindle checkpoint initiation. (springer.com)
  • Diseases associated with EXOSC3 include Pontocerebellar Hypoplasia, Type 1B and Pontocerebellar Hypoplasia Type 1 . (genecards.org)
  • Short-chain fatty acids (SCFAs), which include butyrate, are produced by beneficial colonic bacteria (probiotics) that feed on, or ferment prebiotics, which are plant products that contain adequate amounts of dietary fiber. (wikipedia.org)
  • Blue Loading Pack ( #7722 ) or Red Loading Pack ( #7723 ) Prepare fresh 3X reducing loading buffer by adding 1/10 volume 30X DTT to 1 volume of 3X SDS loading buffer. (cellsignal.com)
  • #4050 ) To prepare 1 L 1X running buffer: add 100 ml 10X running buffer to 900 ml dH 2 O, mix. (cellsignal.com)
  • #12539 ) To prepare 1 L 1X Transfer Buffer: add 100 ml 10X Transfer Buffer to 200 ml methanol + 700 ml dH 2 O, mix. (cellsignal.com)
  • Butyrate is a major metabolite in colonic lumen arising from bacterial fermentation of dietary fiber and has been shown to be a critical mediator of the colonic inflammatory response. (wikipedia.org)
  • Bub1 was originally discovered as a gene required for cell cycle arrest during mitosis in response to the microtubule depolymerizing drug benzimidazole in the model organism Saccharomyces cerevisiae (Hoyt et al. (springer.com)
  • De Iaco A, Santoni F, Vannier A, Guipponi M, Antonarakis S, Luban J. TNPO3 protects HIV-1 replication from CPSF6-mediated capsid stabilization in the host cell cytoplasm. (umassmed.edu)