The extent to which an RNA molecule retains its structural integrity and resists degradation by RNASE, and base-catalyzed HYDROLYSIS, under changing in vivo or in vitro conditions.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
A ZINC FINGER MOTIF containing transcription factor that was originally identified as one of the IMMEDIATE-EARLY PROTEINS. It shuttles between the CYTOPLASM and the CELL NUCLEUS and is involved in destabilization of mRNAs for TUMOR NECROSIS FACTOR-ALPHA.
A subspecialty of internal medicine concerned with the metabolism, physiology, and disorders of the ENDOCRINE SYSTEM.
A heterogeneous-nuclear ribonucleoprotein that has specificity for AU-rich elements found in the 3'-region of mRNA and may play a role in RNA stability. Several isoforms of hnRNP D protein have been found to occur due to alternative mRNA splicing (RNA SPLICING).
A system of safety management (abbreviated HACCP) applied mainly to the food industry. It involves the analysis and control of biological, chemical, and physical hazards, from raw material production, procurement and handling, to manufacturing, distribution and consumption of finished products.
Proteins that bind to RNA molecules. Included here are RIBONUCLEOPROTEINS and other proteins whose function is to bind specifically to RNA.
An intracellular ribonucleolytic protein complex that participates in POSTRANSCRIPTIONAL RNA PROCESSING and RNA DEGRADATION.
A group of inherited and sporadic disorders which share progressive ataxia in combination with atrophy of the CEREBELLUM; PONS; and inferior olivary nuclei. Additional clinical features may include MUSCLE RIGIDITY; NYSTAGMUS, PATHOLOGIC; RETINAL DEGENERATION; MUSCLE SPASTICITY; DEMENTIA; URINARY INCONTINENCE; and OPHTHALMOPLEGIA. The familial form has an earlier onset (second decade) and may feature spinal cord atrophy. The sporadic form tends to present in the fifth or sixth decade, and is considered a clinical subtype of MULTIPLE SYSTEM ATROPHY. (From Adams et al., Principles of Neurology, 6th ed, p1085)
A family of enzymes that catalyze the exonucleolytic cleavage of RNA. It includes EC 3.1.13.-, EC 3.1.14.-, EC 3.1.15.-, and EC 3.1.16.-. EC 3.1.-
Software used to locate data or information stored in machine-readable form locally or at a distance such as an INTERNET site.
Databases devoted to knowledge about specific genes and gene products.
A type of extracellular vesicle, containing RNA and proteins, that is secreted into the extracellular space by EXOCYTOSIS when MULTIVESICULAR BODIES fuse with the PLASMA MEMBRANE.
The complete genetic complement contained in the DNA of a set of CHROMOSOMES in a HUMAN. The length of the human genome is about 3 billion base pairs.
Adverse functional, metabolic, or structural changes in ischemic tissues resulting from the restoration of blood flow to the tissue (REPERFUSION), including swelling; HEMORRHAGE; NECROSIS; and damage from FREE RADICALS. The most common instance is MYOCARDIAL REPERFUSION INJURY.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
An enzyme that catalyzes the conversion of L-alanine and 2-oxoglutarate to pyruvate and L-glutamate. (From Enzyme Nomenclature, 1992) EC 2.6.1.2.
Pathological processes of the LIVER.
A hypoperfusion of the BLOOD through an organ or tissue caused by a PATHOLOGIC CONSTRICTION or obstruction of its BLOOD VESSELS, or an absence of BLOOD CIRCULATION.
Damage to the MYOCARDIUM resulting from MYOCARDIAL REPERFUSION (restoration of blood flow to ischemic areas of the HEART.) Reperfusion takes place when there is spontaneous thrombolysis, THROMBOLYTIC THERAPY, collateral flow from other coronary vascular beds, or reversal of vasospasm.
Derivatives of BUTYRIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxypropane structure.
A RNA-binding protein that binds to polypyriminidine rich regions in the INTRONS of messenger RNAs. Polypyrimidine tract-binding protein may be involved in regulating the ALTERNATIVE SPLICING of mRNAs since its presence on an intronic RNA region that is upstream of an EXON inhibits the splicing of the exon into the final mRNA product.
A family of RNA-binding proteins that are homologues of ELAV protein, Drosophila. They were initially identified in humans as the targets of autoantibodies in patients with PARANEOPLASTIC ENCEPHALOMYELITIS. They are thought to regulate GENE EXPRESSION at the post-transcriptional level.
A family of ribonucleoproteins that were originally found as proteins bound to nascent RNA transcripts in the form of ribonucleoprotein particles. Although considered ribonucleoproteins they are primarily classified by their protein component. They are involved in a variety of processes such as packaging of RNA and RNA TRANSPORT within the nucleus. A subset of heterogeneous-nuclear ribonucleoproteins are involved in additional functions such as nucleocytoplasmic transport (ACTIVE TRANSPORT, CELL NUCLEUS) of RNA and mRNA stability in the CYTOPLASM.
Small double-stranded, non-protein coding RNAs, 21-25 nucleotides in length generated from single-stranded microRNA gene transcripts by the same RIBONUCLEASE III, Dicer, that produces small interfering RNAs (RNA, SMALL INTERFERING). They become part of the RNA-INDUCED SILENCING COMPLEX and repress the translation (TRANSLATION, GENETIC) of target RNA by binding to homologous 3'UTR region as an imperfect match. The small temporal RNAs (stRNAs), let-7 and lin-4, from C. elegans, are the first 2 miRNAs discovered, and are from a class of miRNAs involved in developmental timing.
Complexes of RNA-binding proteins with ribonucleic acids (RNA).
A transcription factor that controls the expression of variety of proteins including CYTOCHROME C and 5-AMINOLEVULINATE SYNTHETASE. It plays an important role in maintenance of the RESPIRATORY CHAIN of MITOCHONDRIA.
A family of transcription factors that control expression of a variety of nuclear GENES encoding proteins that function in the RESPIRATORY CHAIN of the MITOCHONDRIA.
A basic-leucine zipper transcription factor that is involved in regulating inflammatory responses, MORPHOGENESIS, and HEME biosynthesis.
A four carbon acid, CH3CH2CH2COOH, with an unpleasant odor that occurs in butter and animal fat as the glycerol ester.
A family of histone acetyltransferases that is structurally-related to CREB-BINDING PROTEIN and to E1A-ASSOCIATED P300 PROTEIN. They function as transcriptional coactivators by bridging between DNA-binding TRANSCRIPTION FACTORS and the basal transcription machinery. They also modify transcription factors and CHROMATIN through ACETYLATION.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
An enzyme of the transferase class that uses ATP to catalyze the phosphorylation of diacylglycerol to a phosphatidate. EC 2.7.1.107.
Material coughed up from the lungs and expectorated via the mouth. It contains MUCUS, cellular debris, and microorganisms. It may also contain blood or pus.
A disease of chronic diffuse irreversible airflow obstruction. Subcategories of COPD include CHRONIC BRONCHITIS and PULMONARY EMPHYSEMA.
Washing liquid obtained from irrigation of the lung, including the BRONCHI and the PULMONARY ALVEOLI. It is generally used to assess biochemical, inflammatory, or infection status of the lung.
The relationship between the dose of an administered drug and the response of the organism to the drug.
Any disorder marked by obstruction of conducting airways of the lung. AIRWAY OBSTRUCTION may be acute, chronic, intermittent, or persistent.
Inhaling and exhaling the smoke of burning TOBACCO.
Antimicrobial cationic peptides with a highly conserved amino terminal cathelin-like domain and a more variable carboxy terminal domain. They are initially synthesized as preproproteins and then cleaved. They are expressed in many tissues of humans and localized to EPITHELIAL CELLS. They kill nonviral pathogens by forming pores in membranes.
A sustained and usually painful contraction of muscle fibers. This may occur as an isolated phenomenon or as a manifestation of an underlying disease process (e.g., UREMIA; HYPOTHYROIDISM; MOTOR NEURON DISEASE; etc.). (From Adams et al., Principles of Neurology, 6th ed, p1398)
Small cationic peptides that are an important component, in most species, of early innate and induced defenses against invading microbes. In animals they are found on mucosal surfaces, within phagocytic granules, and on the surface of the body. They are also found in insects and plants. Among others, this group includes the DEFENSINS, protegrins, tachyplesins, and thionins. They displace DIVALENT CATIONS from phosphate groups of MEMBRANE LIPIDS leading to disruption of the membrane.
A type of pancreatic cell representing about 50-80% of the islet cells. Beta cells secrete INSULIN.
Irregular microscopic structures consisting of cords of endocrine cells that are scattered throughout the PANCREAS among the exocrine acini. Each islet is surrounded by connective tissue fibers and penetrated by a network of capillaries. There are four major cell types. The most abundant beta cells (50-80%) secrete INSULIN. Alpha cells (5-20%) secrete GLUCAGON. PP cells (10-35%) secrete PANCREATIC POLYPEPTIDE. Delta cells (~5%) secrete SOMATOSTATIN.
Rare congenital metabolism disorders of the urea cycle. The disorders are due to mutations that result in complete (neonatal onset) or partial (childhood or adult onset) inactivity of an enzyme, involved in the urea cycle. Neonatal onset results in clinical features that include irritability, vomiting, lethargy, seizures, NEONATAL HYPOTONIA; RESPIRATORY ALKALOSIS; HYPERAMMONEMIA; coma, and death. Survivors of the neonatal onset and childhood/adult onset disorders share common risks for ENCEPHALOPATHIES, METABOLIC, INBORN; and RESPIRATORY ALKALOSIS due to HYPERAMMONEMIA.
Inflammation of the COLON that is predominantly confined to the MUCOSA. Its major symptoms include DIARRHEA, rectal BLEEDING, the passage of MUCUS, and ABDOMINAL PAIN.
Lining of the INTESTINES, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. In the SMALL INTESTINE, the mucosa is characterized by a series of folds and abundance of absorptive cells (ENTEROCYTES) with MICROVILLI.
Extranodal lymphoma of lymphoid tissue associated with mucosa that is in contact with exogenous antigens. Many of the sites of these lymphomas, such as the stomach, salivary gland, and thyroid, are normally devoid of lymphoid tissue. They acquire mucosa-associated lymphoid tissue (MALT) type as a result of an immunologically mediated disorder.
A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function.
Therapeutic act or process that initiates a response to a complete or partial remission level.
A chronic transmural inflammation that may involve any part of the DIGESTIVE TRACT from MOUTH to ANUS, mostly found in the ILEUM, the CECUM, and the COLON. In Crohn disease, the inflammation, extending through the intestinal wall from the MUCOSA to the serosa, is characteristically asymmetric and segmental. Epithelioid GRANULOMAS may be seen in some patients.
Inflammation of the COLON section of the large intestine (INTESTINE, LARGE), usually with symptoms such as DIARRHEA (often with blood and mucus), ABDOMINAL PAIN, and FEVER.

Hypertrophic stimuli augment expression of cMG1/ERF-1, a putative zinc-finger motif transcription factor, in rat cardiomyocytes. (1/13)

We isolated the gene for cMG1/ERF-1, a known putative zinc-finger transcription factor, by differential display of mRNA extracted from cardiomyocytes with and without leukemia inhibitory factor (LIF) stimulation. LIF induced cMG1/ERF-1 mRNA at 15 min, and levels peaked at 10-fold initial levels at 30 min. cMG1/ERF-1 expression was inhibited by AG490 (JAK2 inhibitor) and genistein, but was unaffected by PD98059 or wortmannin. Phenylephrine, angiotensin II and endothelin-1 also induced cMG1/ERF-1 expression. Mechanical stretch in vitro and acute pressure overload in vivo increased cMG1/ERF-1 expression. To our knowledge, this is the first report showing that the cMG1/ERF-1 gene can be induced by various hypertrophic stimuli, and that Janus kinase 2 is involved in this process.  (+info)

Anti-CD20- and B-cell receptor-mediated apoptosis: evidence for shared intracellular signaling pathways. (2/13)

Clinical administration of the anti-CD20 antibody IDEC-C2B8 can induce remission of low-grade B-cell lymphoma. Whereas it has been suggested that the main mechanisms of action are complement-mediated and antibody-dependent cell-mediated cytotoxicity, we demonstrate that monoclonal antibody IDEC-C2B8 is a strong inducer of apoptosis in CD20-positive B-cell lymphoma cell lines reflecting different stages of lymphomagenesis. Thus, CD20-dependent apoptosis was inducible in human surface IgM-positive Burkitt's lymphoma cell lines as well as in more mature surface IgM-negative B-cell lymphoma cell lines carrying the t(14;18) translocation. Furthermore, in Burkitt's lymphoma cell lines, we observed a striking correlation between anti-CD20- and B-cell receptor-mediated apoptosis with regard to sensitivity toward the apoptotic stimuli and the execution of the apoptotic pathway. Thus, induction of anti-CD20- or B-cell receptor-mediated apoptosis involved rapid up-regulation of the proapoptotic protein Bax. In addition, we show similar changes in the mRNA expression level of two early response genes, c-myc and Berg36, as well as activation of the mitogen-activated protein kinase family members p44 (extracellular signal-regulated kinase 1) and p42 (extracellular signal-regulated kinase 2) and activation of activator protein 1 (AP-1) DNA binding activity. These data support our hypothesis that both pathways are mediated in part by the same signal-transducing molecules. These results might help explain the resistance and regression of lymphomas to IDEC-C2B8 and give new insights in the signaling cascade after CD20 ligation.  (+info)

Functional cloning of BRF1, a regulator of ARE-dependent mRNA turnover. (3/13)

To identify regulators of AU-rich element (ARE)-dependent mRNA turnover we have followed a genetic approach using a mutagenized cell line (slowC) that fails to degrade cytokine mRNA. Accordingly, a GFP reporter construct whose mRNA is under control of the ARE from interleukin-3 gives an increased fluorescence signal in slowC. Here we describe rescue of slowC by a retroviral cDNA library. Flow cytometry allowed us to isolate revertants with reconstituted rapid mRNA decay. The cDNA was identified as butyrate response factor-1 (BRF1), encoding a zinc finger protein homologous to tristetraprolin. Mutant slowC carries frame-shift mutations in both BRF1 alleles, whereas slowB with intermediate decay kinetics is heterozygous. By use of small interfering (si)RNA, independent evidence for an active role of BRF1 in mRNA degradation was obtained. In transiently transfected NIH 3T3 cells, BRF1 accelerated mRNA decay and antagonized the stabilizing effect of PI3-kinase, while mutation of the zinc fingers abolished both function and ARE-binding activity. This approach, which identified BRF1 as an essential regulator of ARE-dependent mRNA decay, should also be applicable to other cis-elements of mRNA turnover.  (+info)

Roles of AUF1 isoforms, HuR and BRF1 in ARE-dependent mRNA turnover studied by RNA interference. (4/13)

HT1080 cells stably expressing green fluorescent protein (GFP) linked to a 3' terminal AU-rich element (ARE) proved to be a convenient system to study the dynamics of mRNA stability, as changes in mRNA levels are reflected in increased or decreased fluorescence intensity. This study examined whether mRNA stability can be regulated by small interfering RNAs (siRNAs) targeted to AU-binding proteins (AUBPs), which in turn should reveal their intrinsic role as stabilizers or destabilizers of ARE-mRNAs. Indeed, siRNAs targeting HuR or BRF1 decreased or increased fluorescence, respectively. This effect was abolished if cells were treated with both siRNAs, thus indicating antagonistic control of ARE-mRNA stability. Unexpectedly, downregulation of all four AUF1 isoforms by targeting common exons did not affect fluorescence whereas selective downregulation of p40AUF1/p45AUF1 strongly increased fluorescence by stabilizing the GFP-ARE reporter mRNA. This observation was fully confirmed by the finding that only selective reduction of p40AUF1/p45AUF1 induced the production of GM-CSF, an endogenous target of AUF1. These data suggest that the relative levels of individual isoforms, rather than the absolute amount of AUF1, determine the net mRNA stability of ARE-containing transcripts, consistent with the differing ARE-binding capacities of the isoforms.  (+info)

Recruitment and activation of mRNA decay enzymes by two ARE-mediated decay activation domains in the proteins TTP and BRF-1. (5/13)

In human cells, a critical pathway in gene regulation subjects mRNAs with AU-rich elements (AREs) to rapid decay by a poorly understood process. AREs have been shown to directly activate deadenylation, decapping, or 3'-to-5' exonucleolytic decay. We demonstrate that enzymes involved in all three of these mRNA decay processes, as well as 5'-to-3' exonucleolytic decay, associate with the protein tristetraprolin (TTP) and its homolog BRF-1, which bind AREs and activate mRNA decay. TTP and BRF-1 each contain two activation domains that can activate mRNA decay after fusion to a heterologous RNA-binding protein, and inhibit ARE-mediated mRNA decay when overexpressed. Both activation domains employ trans-acting factors to trigger mRNA decay, and the N-terminal activation domain functions as a binding platform for mRNA decay enzymes. Our data suggest that the TTP protein family functions as a molecular link between ARE-containing mRNAs and the mRNA decay machinery by recruitment of mRNA decay enzymes, and help explain how deadenylation, decapping, and exonucleolytic decay can all be independently activated on ARE-containing mRNAs. This describes a potentially regulated step in activation of mRNA decay.  (+info)

Mitogen-induced expression of the primary response gene cMG1 in a rat intestinal epithelial cell-line (RIE-1). (6/13)

cMG1 is a primary response gene first identified in a rat intestinal epithelial (RIE-1) cell-line [(1990) Oncogene 5, 1081-1083]. A number of mitogens, including epidermal growth factor (EGF), angiotensin II (AII), serum and insulin rapidly induced 2- to 6-fold increases of cMG1 mRNA in RIE-1 cells, while transforming growth factor-beta caused a small reduction. Cyclic AMP-elevating agents blocked the increase of cMG1 mRNA induced by EGF. The AII-stimulated increase of cMG1 mRNA was blocked by the depletion of protein kinase C, whereas the EGF-stimulated increase was not affected, indicating that protein kinase C-dependent and -independent signalling pathways stimulate cMG1 expression.  (+info)

Von Hippel-Lindau gene product modulates TIS11B expression in renal cell carcinoma: impact on vascular endothelial growth factor expression in hypoxia. (7/13)

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ZFP36L1 negatively regulates erythroid differentiation of CD34+ hematopoietic stem cells by interfering with the Stat5b pathway. (8/13)

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The histone lysine methyltransferase nuclear receptor-binding SET domain protein 2 (NSD2, also known as WHSC1/MMSET) is an epigenetic modifier and thought to play a driving role in oncogenesis. Both NSD2 overexpression and point mutations that increase its catalytic activity are associated with several human cancers. While NSD2 is an attractive therapeutic target, no potent, selective, and bioactive small molecule inhibitors of NSD2 have been reported to date, possibly due to the challenges of developing high-throughput assays for NSD2. Here, to establish a platform for the discovery and development of selective NSD2 inhibitors, we optimized and implemented multiple assays. We performed quantitative high-throughput screening with full-length wild-type NSD2 and a nucleosome substrate against a diverse collection of bioactive small molecules comprising 16,251 compounds. We further interrogated 174 inhibitory compounds identified in the primary screen with orthogonal and counter assays and with ...
May play a role in subcellular trafficking between the endoplasmic reticulum and Golgi apparatus through interactions with the Rho-type GTPases. Binding to the NS3 protein of dengue virus type 2 appears to subvert this activity into the alteration of the intracellular membrane structure associated with flaviviral replication.
Risk Profile: Moderate Growth The CMG Tactical Rotation Strategy seeks to generate returns in all market conditions based on the concept that various
Regulation of mRNA turnover is an important process in determining levels of gene expression. mRNA stability varies considerably from one mRNA species to another and is determined by specific cis-acting elements within the mRNA molecule. mRNAs encoding cytokines and proto-oncogenes are degraded rapidly in order to minimize potentially inflammatory or oncogenic effects that may result from their overexpression. Many of these transcripts contain cis-acting instability elements within their 3 untranslated regions that are believed to activate mRNA decay pathways. The AU-rich elements (AREs) appear to be prominent elements that direct rapid mRNA decay by a process referred to as ARE-mediated mRNA decay (AMD). AMD is regulated by RNA-binding proteins. A number of proteins have been described to bind AREs and are collectively called ARE-binding proteins (ARE-BPs). Decay-promoting ARE-BPs bind ARE-containing mRNAs and target them for decay by recruitment of mRNA decay enzymes. Our research is directed ...
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein containing a SET domain, 2 LXXLL motifs, 3 nuclear translocation signals (NLSs), 4 plant homeodomain (PHD) finger regions, and a proline-rich region. The encoded protein enhances androgen receptor (AR) transactivation, and this enhancement can be increased further in the presence of other androgen receptor associated coregulators. This protein may act as a nucleus-localized, basic transcriptional factor and also as a bifunctional transcriptional regulator. Mutations of this gene have been associated with Sotos syndrome and Weaver syndrome. One version of childhood acute myeloid leukemia is the result of a cryptic translocation with the breakpoints occurring within nuclear receptor-binding Su-var, enhancer of zeste, and trithorax domain protein 1 on chromosome 5 and nucleoporin, 98-kd on chromosome 11. Two transcript variants encoding distinct isoforms have been identified ...
This gene encodes a protein containing a SET domain, 2 LXXLL motifs, 3 nuclear translocation signals (NLSs), 4 plant homeodomain (PHD) finger regions, and a proline-rich region. The encoded protein enhances androgen receptor (AR) transactivation, and this enhancement can be increased further in the presence of other androgen receptor associated coregulators. This protein may act as a nucleus-localized, basic transcriptional factor and also as a bifunctional transcriptional regulator. Mutations of this gene have been associated with Sotos syndrome and Weaver syndrome. One version of childhood acute myeloid leukemia is the result of a cryptic translocation with the breakpoints occurring within nuclear receptor-binding Su-var, enhancer of zeste, and trithorax domain protein 1 on chromosome 5 and nucleoporin, 98-kd on chromosome 11. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008 ...
BioInvenu provides custom cell line generation services to meet your specific needs in high throughput screening.. Stable Cell lines. ...
Chinas demand for phenol has grown at a fast pace in the past decade. In the next five years, both production and demand will continue to grow. This new study examines Chinas economic trends, investment environment, industry development, supply and demand, industry capacity, industry structure, marketing channels and major industry participants. Historical data (2002, 2007 and 2012) and long-term forecasts through 2017 and 2022 are presented. Major producers in China are profiled ...
RecName: Full=A-kinase anchor protein 13; Short=AKAP-13;AltName: Full=Protein kinase A-anchoring protein 13;AltName: Full=Breast cancer nuclear receptor-binding auxiliary protein;AltName: Full=Human thyroid-anchoring protein 31;AltName: Full=Guanine nucleotide exchange factor Lbc;AltName: Full=AKAP-Lbc;AltName: Full=P47;AltName: Full=Lymphoid blast crisis oncogene; Short=LBC oncogene;AltName: Full=Non-oncogenic Rho GTPase-specific GTP exchange ...
This directory for the Case Mix Group+ (CMG+) inpatient grouping methodology contains flowcharts of the CMG+ logic and lists the ICD-10-CA diagnosis and CCI procedure codes used in the assignment of MCC and CMG cells. ...
Purpose Chronic Lymphocytic Leukemia (CLL) is normally described by a perturbed B-cell receptor-mediated signaling machinery. the PLSR response adjustable within the C cell people is normally both a quality tag of healthful examples and predictive of disease aggressiveness. Bottom line Single-cell multidimensional evaluation of BCR signaling allowed concentrated evaluation of the variability and heterogeneity of signaling behavior from patient-to-patient, and from cell-to-cell. Interruption of the pSYK/pPLC2 romantic relationship is normally open as a sturdy trademark of CLL C cell signaling behavior. Jointly, these findings implicate story components of the BCR indication transduction as potential healing goals. Launch Chronic lymphocytic leukemia (CLL) outcomes from the deposition of older monoclonal Compact disc5+ C cells in the bone fragments marrow, lymphoid areas and peripheral bloodstream. CLL C cells are characterized by low appearance of surface area Compact disc20 and co-expression of ...
RSV and hMPV infection modulates ARE-dependent gene transcription(A) A549 cells were transiently transfected with a plasmid containing multiple copies of the NQ
Czech myeloma group represents the association of doctors, specialists and other research workers whose aim is to examine and diagnose a disease called multiple myeloma. The associations broad objective is the diagnostics of diseases belonging to a group called monoclonal gamapathy. CMG organizes educational, experimental and other collective activities.
Czech myeloma group represents the association of doctors, specialists and other research workers whose aim is to examine and diagnose a disease called multiple myeloma. The associations broad objective is the diagnostics of diseases belonging to a group called monoclonal gamapathy. CMG organizes educational, experimental and other collective activities.
Get News First. Profit Faster With Benzinga Professional get unlimited access to ALL content! PLUS: REALTIME NEWSFEED REALTIME AUDIO NEWS FULL CALENDAR ...
InfoBank, KnowledgeBanks field data catalog, is a structured information storage scheme of databases and software that provide organized access to USGS Coastal and Marine data and metadata.
InfoBank, KnowledgeBanks field data catalog, is a structured information storage scheme of databases and software that provide organized access to USGS Coastal and Marine data and metadata.
is it possible to optimize a transfected cell-line (by G418-selection) by subcloning, i. e. to get a better receptor expession. I think if i got a monoclonal mammalial cell-line which is overexpressing a receptor gene, it could improve the expression of this gene, to make a subcloning by dilution. I think the cells are dividing over the time and are so not longer be monoclonal ...
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KMT3B / NSD1兔多克隆抗体(ab84137)可与人样本反应并经IHC实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
Royalty free stock sound clip for personal, commercial, production use a young boy coughing. 0:01 / 1009.1 KB / $2.99. Download now on Pond5 |||
The Positive Transcription Elongation Factor b (P-TEFb) is a complex of Cyclin Dependent Kinase 9 (CDK9) with either cyclins T1, T2 or K. The complex phosphorylates the C-Terminal Domain of RNA polymerase II (RNAPII) and negative elongation factors, stimulating productive elongation by RNAPII, which is paused after initiation. P-TEFb is recruited downstream of the promoters of many genes, including primary response genes, upon certain stimuli. Flavopiridol (FVP) is a potent pharmacological inhibitor of CDK9 and has been used extensively in cells as a means to inhibit CDK9 activity. Inhibition of P-TEFb complexes has potential therapeutic applications. It has been shown that Lipopolysaccharide (LPS) stimulates the recruitment of P-TEFb to Primary Response Genes (PRGs) and proposed that P-TEFb activity is required for their expression, as the CDK9 inhibitor DRB prevents localization of RNAPII in the body of these genes. We have previously determined the effects of FVP in global gene expression in a
EXCLUSIVE: Gateway Films & Prana Studios 3D animated feature opened in second place at the Polish box office last week behind The Hunger Games: Catching Fire.
There is an industry need for new high performance technologies that achieve the goal of meeting the unmet needs of bio-manufacturing. These technologies must be: effective, safe, have decent capacity, rapid response, be cost-effective and simple to manufacture. Fraunhofer USA are attempting to produce these technologies. Pilot production has operated to USFDA cGMP standards. Should the technologies be biological- can the system produce proteins with desired characteristics? Should the technologies be commercial- do they attributes that make the technology competitive? Should the technologies be regulatory- are they safe, potent and effective? Tell us your opinion by commenting in the space provided below! Dr Vidadi Yusibov, Executive Director, Fraunhofer USA joined us at the World Vaccine Congress 2012 in Washington to discuss whether or not one cell-line can meet the demands of product quantities and varieties. Why not download the full presentation to find out more about: ...
Cell-line quality has a significant impact on biologic drug quality; learn more about this and other upstream challenges at the new bioLIVE launching this year adjacent to CPhI Worldwide 2018.
NSD Therapy is the most advanced form is spinal rehabilitation in the World today. Learn the Contraindications to NSD Therapy today.
TY - JOUR. T1 - Phthalate plasticizer di(2-ethyl-hexyl) phthalate induces cyclooxygenase-2 expression in gastric adenocarcinoma cells. AU - Wong, Jhen Hong. AU - Wang, Yu Shiuan. AU - Nam, Sean. AU - Ho, Kuo Hao. AU - Chang, Che Mai. AU - Chen, Ku Chung. AU - Chen, Yi Fan. AU - Chang, Wei Chiao. PY - 2019/1/1. Y1 - 2019/1/1. N2 - The phthalate plasticizer, di(2-ethyl-hexyl) phthalate (DEHP), and its derived metabolites are common anthropogenic environmental toxins, which are known to act as endocrine disruptors. Numerous studies have associated DEHP with disruption of sex hormones, abnormal development of reproductive organs, allergies, and inflammation. Its role in promoting inflammation has been reported by both human epidemiological and animal studies. In stomach tissue, chronic inflammation is known to accompany mucosal damage, and pave the way to gastritis, stomach ulcers, and ultimately gastric cancer. Eastern Asian populations possess the highest gastric cancer incidences in the world. ...
Introduction: Over the past decade potatoes with purple- and red-fleshed color rich in antioxidants (phenolic compounds - phenolic acids, anthocyanins etc.) have attracted the attention of consumers and scientists due to their appearance, flavour and biological properties. It is assumed that they have the potential to be an important source of biologically active compounds in the human nutrition and beneficial for human health. The aim of this study was to investigate whether potato tubers with coloured flesh might suppress human gastric adenocarcinoma cells (AGS cells) proliferation and viability. Object and methods: The field experiment was conducted in 2017 and 2018 on farm in Širvintos district (Lithuania). The study was implemented with 5 potato cultivars (Solanum tuberosum L) with different coloured flesh. In the conventional farming system the mix of universal complex fertilizers (Blaukorn Novatec N14P7K17) was used during potato crops vegetative period by inserting the 112 kg ha-1 ...
E4orf6 exports and stabilizes ARE-mRNAs. (A) HuR, which bound to mRNA, was isolated from the nuclear (N) and cytoplasmic (C) fractions of each BRK cell exposed to UV light by oligo (dT)-cellulose chromatography (left). The amount of HuR in total extract (TE) of each cell is shown (middle). The fractions and TE were analyzed by immunoblotting with antibodies to β-tubulin and poly(ADP-ribose)polymerase (PARP; right). (B) The amount of c-fos, c-myc, COX-2, and GAPDH mRNA expressed in the cytoplasm of each cell was measured by quantitative real-time RT-PCR. BRK E1 and E1+E4 cells (left), 293 cells transfected with E4orf6 expression vector (middle), and HeLa cells infected with Ad dl309 and Ad dl355 (right) were used. (C) pCMVGL-ARE, which has ARE of c-fos in 3′-UTR of luciferase cDNA (left), were transfected into 293 cells with or without E4orf6 expression construct, and then the accumulation of the cytoplasmic luciferase mRNA was analyzed by Northern blot 24 h after the transfection. The ...
Transforming growth factor β1 (TGF-β1) is a potent growth inhibitor for many cell types, including tumor cells. We recently have reported the establishment and characterization of two human gastric scirrhous carcinoma cell lines, HSC-39 and HSC-43. Here we examined the effect of TGF-β1 on the growth of these lines as compared to five other human gastric adenocarcinoma cell lines. Proliferation of HSC-39 and HSC-43 cells was strongly inhibited by TGF-β1, whereas the other gastric adenocarcinoma cell lines were unresponsive to TGF-β1. Both HSC-39 and HSC-43 cells gradually lost viability following exposure to TGF-β1. This response was dose dependent up to 4 ng/ml. When TGF-β1 was removed, the cells failed to exhibit regrowth, indicating an irreversible growth-inhibitory effect of this agent, leading to cell death. DNA fragments were observed consisting of multimers of approximately 180 base pairs 24 h after TGF-β1 treatment. The chromatin condensation of each cell line was confirmed by ...
Here, we investigated the effect of bacterial metabolites produced by commensal bacteria on AP-1 signaling pathway … ... Transcription Factor AP-1 / metabolism* * Transcription Factor AP-1 / physiology * Transcriptional Activation ... Butyrate produced by commensal bacteria potentiates phorbol esters induced AP-1 response in human intestinal epithelial cells ... Moreover, butyrate enhanced the PMA-induced expression of c-fos and ERK1/2 phosphorylation, but not p38 and JNK. In conclusion ...
BUTYRATE RESPONSE FACTOR 2. C. 9. Homo sapiens. Mutation(s): 0 Gene Names: ZFP36L2, ERF2, RNF162C, TIS11D. ... and X-ray crystallographic analyses of both HLA-B27 subtypes complexed with the epidermal growth factor response factor 1- ... Crystal Structure Of HLA-B*2709 Complexed With the self-Peptide TIS from EGF-response factor 1. *DOI: 10.2210/pdb1W0W/pdb ... As peptide presentation by HLA molecules is of central importance for immune responses, we performed thermodynamic (circular ...
This process occurs in response to modifications of the cellular environment, including hormonal variations, and regulates the ... This process occurs in response to modifications of the cellular environment, including hormonal variations, and regulates the ... TIS11b, TPA-inducible sequence 11b; BRF1, butyrate response factor 1; ZFP36, zinc finger protein; TTP, tristetraprolin; CCR4, ... through stabilization of cAMP response element-binding protein (CREB) transcription factor. J Biol Chem (2011) 286:32976-85. ...
... and butyrate response factor 2 (BRF2; also known as EGF response factor 2 [ERF2], TIS11D, ZFP36L2) both belong to the TIS11 ... Butyrate response factor 1 (BRF1; also known as EGF response factor 1 [ERF1], TIS11B, ZFP36L1) ... growth factors and cell cycle regulators, including GM-CSF, TNFα, IL-2, IL-3 and IL-6 (4,5). Deregulated ARE-mRNA stability can ... 10X Tris Buffered Saline with Tween® 20 (TBST): (#9997) To prepare 1 L 1X TBST: add 100 ml 10X TBST to 900 ml dH2O, mix. ...
ZFP36 Ring Finger Protein Like 1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - ... Butyrate response factor 1 is regulated by parathyroid hormone and bone morphogenetic protein-2 in osteoblastic cells. (PMID: ... This putative nuclear transcription factor most likely functions in regulating the response to growth factors. Alternatively ... Up-regulated by growth factor (TGF-beta), cytokines, tumor necrosis factor (TNF-alpha) and epidermal growth factor (EGF) in ...
Compound: Butyrate response factor 2. Database cross-references and differences (RAF-indexed): *Uniprot Q07352 (0-8) * ... Description: crystal structure of hla-b*2709 complexed with the self-peptide tis from egf-response factor 1. Class: immune ... R-factor: 0.1857. AEROSPACI score: 0.44 (click here for full SPACI score report) Chains and heterogens:. *Chain A:. Compound ... Uniprot P61769 (1-99) Domains in SCOP 1.73: d1w0wb1*Chain C:. ...
Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA, organism-specific biosystem (from REACTOME) Butyrate Response ... organism-specific biosystemButyrate Response Factor 1 (BRF1, ZFP36L1, not to be confused with transcription factor IIIB) binds ... Unfolded Protein Response (UPR), organism-specific biosystemThe Unfolded Protein Response (UPR) is a regulatory system that ... ATF4 activates genes, organism-specific biosystemATF4 is a transcription factor and activates expression of IL-8, MCP1, IGFBP-1 ...
Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA, organism-specific biosystem (from REACTOME) Butyrate Response ... Transcription factor Foxp3 and its protein partners form a complex regulatory network. Rudra D, et al. Nat Immunol, 2012 Oct. ... Conserved Domains (1) summary. cd11367. Location:5 → 276. RNase_PH_RRP42; RRP42 subunit of eukaryotic exosome. ... Conserved Domains (1) summary. cd11367. Location:8 → 198. RNase_PH_RRP42; RRP42 subunit of eukaryotic exosome. ...
cellular response to transforming growth factor beta stimulus Source: Ensembl. *cellular response to tumor necrosis factor ... cellular response to butyrate Source: Ensembl. *cellular response to interferon-gamma Source: Ensembl ... 1 - 211. Claudin-1Add BLAST. 211. Amino acid modifications. Feature key. Position(s). DescriptionActions. Graphical view. ... Last modified:November 1, 1998 - v1. ,p>The checksum is a form of redundancy check that is calculated from the sequence. It is ...
Exosome Component 1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene ... Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA. *Deadenylation-dependent mRNA decay ... Transcription Factor. Binding Sites. Gene Targets. GH10J097444. Promoter/Enhancer. 2. EPDnew Ensembl ENCODE CraniofacialAtlas ... 1. ^. 2a. ·. 2b. ^. 3. ^. 4. ^. 5a. ·. 5b. ^. 6a. ·. 6b. ^. 7a. ·. 7b. ^. 8a. ·. 8b. ^. 9a. ·. 9b. ·. 9c. ...
Butyrate response factor 1 is a protein that in humans is encoded by the ZFP36L1 gene. This gene is a member of the TIS11 ... Reppe S, Olstad OK, Rian E, Gautvik VT, Gautvik KM, Jemtland R (November 2004). "Butyrate response factor 1 is regulated by ... This RNA binding protein most likely functions in regulating the response to growth factors. ZFP36L1 can degrade transcripts of ... The gene is well conserved across species and has a promoter that contains motifs seen in other early-response genes. The ...
R-SCE-450385 Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA. R-SCE-450513 Tristetraprolin (TTP, ZFP36) binds and ... R-SCE-450385 Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA. R-SCE-450513 Tristetraprolin (TTP, ZFP36) binds and ... 3.30.230.70, 1 hit. InterProi. View protein in InterPro. IPR001247 ExoRNase_PH_dom1. IPR015847 ExoRNase_PH_dom2. IPR036345 ... Q05636-1 [UniParc]FASTAAdd to basketAdded to basket. « Hide. 10 20 30 40 50. MAKDIEISAS ESKFILEALR QNYRLDGRSF DQFRDVEITF ...
It is a member of the TIS11 (TPA-induced sequence) family, along with butyrate response factors 1 and 2. TTP binds to AU-rich ... DuBois RN, McLane MW, Ryder K, Lau LF, Nathans D (Dec 1990). "A growth factor-inducible nuclear protein with a novel cysteine/ ... Carballo E, Lai WS, Blackshear PJ (August 1998). "Feedback inhibition of macrophage tumor necrosis factor-alpha production by ... a potential zinc finger transcription factor, by mitogen stimulation in intact cells and by mitogen-activated protein kinase in ...
2013) Transcription factors and genetic circuits orchestrating the complex, multilayered response of Clostridium acetobutylicum ... The butyrate-producing phenotype was also observed for the same strain when pH was controlled above 6.0 (SI Appendix, Fig. S10 ... In response to the substantial decrease of the pH, cells enter the stationary phase, and the organic acids formed are ... 4A, Right) gave rise to excessive amounts of butyrate but minimal acetate and the solvents, although cell growth remained ...
101710075004 Butyrate Response Factor 1 Proteins 0.000 description 4 * 101710020283 CD14 Proteins 0.000 description 4 ... 102000003995 transcription factors Human genes 0.000 description 15 * 108090000464 transcription factors Proteins 0.000 ... 230000028993 immune response Effects 0.000 claims description 7 * 108010043412 neuropeptide Y-Y1 receptor Proteins 0.000 claims ... 101710007275 Nuclear Respiratory Factor 1 Proteins 0.000 claims description 12 * 101710063427 PDE3A Proteins 0.000 claims ...
Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA (Homo sapiens) * p-S54,92,203-BRF1 binds 14-3-3 (Homo sapiens) * ... 14-3-3 protein beta/alpha, 143B protein, Protein kinase C inhibitor protein-1, KCIP-1, Protein 1054 ...
We conclude that butyrate inhibition of endotoxin translocation, macrophages activation, inflammatory factors production, and ... The inflammatory response aggravated by endotoxin after I/R contributes to liver dysfunction and failure. The purpose of the ... and neutrophil infiltration in live were attenuated by butyrate. Butyrate also maintained the intestinal barrier structures, ... Butyrate protects rat liver against total hepatic ischemia reperfusion injury with bowel congestion.. [Bin Liu, Jianmin Qian, ...
... butyrate response factor 1), a probable regulatory protein involved in regulating the response to growth factors, and the mouse ... The mouse TTP protein is induced by growth factors. Another protein containing this domain is the human splicing factor U2AF ... They were first identified as a DNA-binding motif in transcription factor TFIIIA from Xenopus laevis (African clawed frog), ... Feedback inhibition of macrophage tumor necrosis factor-alpha production by tristetraprolin.. Science 281 1001-5 1998 ...
Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA BibTex 2010-06-29 R-HSA-450513 Tristetraprolin (TTP, ZFP36) binds ...
Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA (Homo sapiens) * p-S92,203-BRF1 binds 14-3-3 (Homo sapiens) * ...
Ab and autoantibody responses. We show that histone deacetylase inhibitors valproic acid and butyrate dampened AICDA/Aicda (AID ... Plasmacytic transcription factor Blimp-1 is repressed by Bach2 in B cells. J. Biol. Chem. 281: 38226-38234. ... CSR and SHM in Ab responses are inhibited by HDIs. To address the effect of HDIs on a T-dependent response, we injected C57BL/6 ... VPA and butyrate (obtained as sodium butyrate from Sigma-Aldrich) were directly diluted in culture media for in vitro ...
They are shown to modulate a variety of immune or nonimmune host cell responses. However, how cathelicidins are expressed by ... CRAMP promotes b-cell survival in vitro via the epidermal growth factor receptor (EGFR) and by modulating expression of ... CRAMP expression is inducible by butyrate or phenylbutyric acid and its secretion triggered upon inflammatory challenges by IL- ... Additional investigation under inflammatory conditions reveals that CRAMP modulates inflammatory responses and beta-cell ...
... gene under the control of GalNAc-T3 indicated that several transcriptional elements are involved in response to sodium butyrate ... p300/CBP-associated factor (P/CAF) interacts with nuclear respiratory factor-1 to regulate the UDP-N-acetyl-alpha-d- ... p300/CBP-associated factor (P/CAF) interacts with nuclear respiratory factor-1 to regulate the UDP-N-acetyl-alpha-d- ... p300/CBP-associated factor (P/CAF) interacts with nuclear respiratory factor-1 to regulate the UDP-N-acetyl-alpha-d- ...
... changes in gene expression programs are strongly influenced by post-transcriptional mechanisms mediated by mRNA-binding factors ... we review the RBPs and miRNAs that modulate mRNA turnover and translation in response to hypoxic challenge. RBPs such as HuR ( ... we review the RBPs and miRNAs that modulate mRNA turnover and translation in response to hypoxic challenge. RBPs such as HuR ( ... iron-response element binding proteins (IRPs), and cytoplasmic polyadenylation-element-binding proteins (CPEBs), selectively ...
No mention of vaccines - a major factor in at least some autism.. Clostridium butyricum produces butyrate. Its available as ... Adaptive responses are normal responses that are appropriate especially in short-term response to a particular threat. But all ... The Cell Danger Response. Then the third theory that they mention is the cell danger response. This is something that has been ... The stress response is very helpful when were exposed to a threat because this fight-or-flight response helps us to either run ...
... and it is required for butyrate effects. We conclude that butyrate protects intestinal epithelial cells from damage caused by C ... The inflammatory response to transformed cells forms the cornerstone of natural or therapeutically-induced protective immunity ... Activation of the transcription factor HIF-1 in intestinal epithelial cells exerts a protective effect in C. difficile-induced ... Butyrate Protects Mice from Clostridium difficile-Induced Colitis through an HIF-1-Dependent Mechanism.. Fachi JL, Felipe JS, ...
... biological response modifiers and the family of cell adhesion-promoting molecules. ... In the liver, propionate acts as a gluconeogenic factor while acetate and butyrate act as lipogenic factors [96]. SCFAs, ... K. Ganeshan and A. Chawla, "Metabolic regulation of immune responses," Annual Review of Immunology, vol. 32, no. 1, pp. 609-634 ... β mRNA in M1 macrophages stimulated with LPS leads to activation of the transcription factor hypoxia-induced factor 1α (HIF1α ...
Impaired glucose response is a significant risk factor for T2DM. Thus, maintaining good blood glucose response is considered ... Prediabetes, characterized by chronically impaired blood glucose response, is a significant risk factor for T2DM with up to 70 ... proposed that the heterogeneous responses in butyrate concentrations upon RS supplementation may be explained by the ... which was similar to our observation of the deteriorated glucose response following the decreased butyrate-producing bacteria ...
... and Odoribacter expressed enzymes favoring the production of butyrate over propionate. Butyrate production by Firmicutes might ... The last factor explaining the gradual colonization of the chicken cecum is that of spore formation. Anaerostipes, ... Differential carbon source utilization by Campylobacter jejuni 11168 in response to growth temperature variation. J Microbiol ... Revealing the bacterial butyrate synthesis pathways by analyzing (meta)genomic data. mBio 5:e00889. doi:10.1128/mBio.00889-14. ...
Butyrate inhibits inflammatory responses through NFkappaB inhibition: implications for Crohns disease. Gut 2000;47:397-403. ... SLC16A1 encoding for the butyrate receptor, the aquaporins AQP3 and AQP8, the trefoil factor TFF1, and those genes encoding for ... Impaired butyrate oxidation in ulcerative colitis is due to decreased butyrate uptake and a defect in the oxidation pathway. ... Another interesting target identified in this study is the SLC16A1 gene encoding for the butyrate receptor. Butyrate is a ...

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