A water-soluble aminoglycosidic antibiotic complex isolated from fermentation filtrates of Bacillus circulans. Two components (A and B) have been separated from the complex. Both are active against many gram-positive and some gram-negative bacteria.
A broad-spectrum antimicrobial isolated from Streptomyces ribosifidicus.
A component of NEOMYCIN that is produced by Streptomyces fradiae. On hydrolysis it yields neamine and neobiosamine B. (From Merck Index, 11th ed)
Glycosylated compounds in which there is an amino substituent on the glycoside. Some of them are clinically important ANTIBIOTICS.
A genus of BACILLACEAE that are spore-forming, rod-shaped cells. Most species are saprophytic soil forms with only a few species being pathogenic.
Antibiotic complex produced by Streptomyces kanamyceticus from Japanese soil. Comprises 3 components: kanamycin A, the major component, and kanamycins B and C, the minor components.
A heteropolysaccharide that is similar in structure to HEPARIN. It accumulates in individuals with MUCOPOLYSACCHARIDOSIS.
A class of enzymes that inactivate aminocyclitol-aminoglycoside antibiotics (AMINOGLYCOSIDES) by regiospecific PHOSPHORYLATION of the 3' and/or 5' hydroxyl.
Substances that reduce the growth or reproduction of BACTERIA.
Antibiotic complex produced by Streptomyces fradiae. It is composed of neomycins A, B, and C. It acts by inhibiting translation during protein synthesis.
The terms, expressions, designations, or symbols used in a particular science, discipline, or specialized subject area.
Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however CATALYTIC RNA and CATALYTIC DNA molecules have also been identified.
International organizations which provide health-related or other cooperative services.
A discipline concerned with studying biological phenomena in terms of the chemical and physical interactions of molecules.
Databases devoted to knowledge about specific genes and gene products.
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.
The interaction of persons or groups of persons representing various nations in the pursuit of a common goal or interest.
A syndrome characterized by central nervous system dysfunction in association with LIVER FAILURE, including portal-systemic shunts. Clinical features include lethargy and CONFUSION (frequently progressing to COMA); ASTERIXIS; NYSTAGMUS, PATHOLOGIC; brisk oculovestibular reflexes; decorticate and decerebrate posturing; MUSCLE SPASTICITY; and bilateral extensor plantar reflexes (see REFLEX, BABINSKI). ELECTROENCEPHALOGRAPHY may demonstrate triphasic waves. (From Adams et al., Principles of Neurology, 6th ed, pp1117-20; Plum & Posner, Diagnosis of Stupor and Coma, 3rd ed, p222-5)
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
An antibiotic produced by the soil actinomycete Streptomyces griseus. It acts by inhibiting the initiation and elongation processes during protein synthesis.
A malabsorption syndrome that is associated with a blind loop in the upper SMALL INTESTINE that is characterized by the lack of peristaltic movement, stasis of INTESTINAL CONTENTS, and the overgrowth of BACTERIA. Such bacterial overgrowth interferes with BILE SALTS action, FATTY ACIDS processing, MICROVILLI integrity, and the ABSORPTION of nutrients such as VITAMIN B12 and FOLIC ACID.
Any tests done on exhaled air.
A colorless alkaline gas. It is formed in the body during decomposition of organic materials during a large number of metabolically important reactions. Note that the aqueous form of ammonia is referred to as AMMONIUM HYDROXIDE.
Glycoproteins with a molecular weight of approximately 620,000 to 680,000. Precipitation by electrophoresis is in the alpha region. They include alpha 1-macroglobulins and alpha 2-macroglobulins. These proteins exhibit trypsin-, chymotrypsin-, thrombin-, and plasmin-binding activity and function as hormonal transporters.
Exclusive legal rights or privileges applied to inventions, plants, etc.
A novel composition, device, or process, independently conceived de novo or derived from a pre-existing model.
Property, such as patents, trademarks, and copyright, that results from creative effort. The Patent and Copyright Clause (Art. 1, Sec. 8, cl. 8) of the United States Constitution provides for promoting the progress of science and useful arts by securing for limited times to authors and inventors, the exclusive right to their respective writings and discoveries. (From Black's Law Dictionary, 5th ed, p1014)
Time period from 1701 through 1800 of the common era.
Body of knowledge related to the use of organisms, cells or cell-derived constituents for the purpose of developing products which are technically, scientifically and clinically useful. Alteration of biologic function at the molecular level (i.e., GENETIC ENGINEERING) is a central focus; laboratory methods used include TRANSFECTION and CLONING technologies, sequence and structure analysis algorithms, computer databases, and gene and protein structure function analysis and prediction.
Time period from 1601 through 1700 of the common era.
Enzymes that catalyze the formation of a carbon-carbon double bond by the elimination of AMMONIA. EC 4.3.1.
An enzyme that catalyzes the deamination of PHENYLALANINE to form trans-cinnamate and ammonia.
An enzyme that catalyzes the deamination of ethanolamine to acetaldehyde. EC 4.3.1.7.
An enzyme that catalyzes the first step of histidine catabolism, forming UROCANIC ACID and AMMONIA from HISTIDINE. Deficiency of this enzyme is associated with elevated levels of serum histidine and is called histidinemia (AMINO ACID METABOLISM, INBORN ERRORS).
An enzyme that catalyzes the conversion of aspartic acid to ammonia and fumaric acid in plants and some microorganisms. EC 4.3.1.1.
An essential aromatic amino acid that is a precursor of MELANIN; DOPAMINE; noradrenalin (NOREPINEPHRINE), and THYROXINE.
Research that involves the application of the natural sciences, especially biology and physiology, to medicine.
A province of Canada lying between the provinces of Manitoba and Quebec. Its capital is Toronto. It takes its name from Lake Ontario which is said to represent the Iroquois oniatariio, beautiful lake. (From Webster's New Geographical Dictionary, 1988, p892 & Room, Brewer's Dictionary of Names, 1992, p391)
A large collection of DNA fragments cloned (CLONING, MOLECULAR) from a given organism, tissue, organ, or cell type. It may contain complete genomic sequences (GENOMIC LIBRARY) or complementary DNA sequences, the latter being formed from messenger RNA and lacking intron sequences.
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
Cements that act through infiltration and polymerization within the dentinal matrix and are used for dental restoration. They can be adhesive resins themselves, adhesion-promoting monomers, or polymerization initiators that act in concert with other agents to form a dentin-bonding system.
Specialized solutions for PARENTERAL NUTRITION. They may contain a variety of MICRONUTRIENTS; VITAMINS; AMINO ACIDS; CARBOHYDRATES; LIPIDS; and SALTS.
Critical and exhaustive investigation or experimentation, having for its aim the discovery of new facts and their correct interpretation, the revision of accepted conclusions, theories, or laws in the light of newly discovered facts, or the practical application of such new or revised conclusions, theories, or laws. (Webster, 3d ed)
Nonsusceptibility of bacteria to the antibiotic KANAMYCIN, which can bind to their 70S ribosomes and cause misreading of messenger RNA.
The process of finding chemicals for potential therapeutic use.
Large collections of small molecules (molecular weight about 600 or less), of similar or diverse nature which are used for high-throughput screening analysis of the gene function, protein interaction, cellular processing, biochemical pathways, or other chemical interactions.
Bacteria which retain the crystal violet stain when treated by Gram's method.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A family of gram-negative bacteria usually found in soil or water and including many plant pathogens and a few animal pathogens.
A family of gram-negative, aerobic bacteria in the order Pseudomonadales. Some strains are parasites of the mucosal membranes of animals and humans; others are found in association with food products or in the environment.
Theory and development of COMPUTER SYSTEMS which perform tasks that normally require human intelligence. Such tasks may include speech recognition, LEARNING; VISUAL PERCEPTION; MATHEMATICAL COMPUTING; reasoning, PROBLEM SOLVING, DECISION-MAKING, and translation of language.
A species of gram-negative bacteria in the genus PASTEURELLA, found in the NASOPHARYNX of normal GUINEA PIGS; RATS; HAMSTERS; MICE; DOGS; and CATS. When associated with disease, it is usually a secondary invader. Occasional infections have been reported in humans.
A family of gram-negative, facultatively anaerobic, rod-shaped bacteria that do not form endospores. Its organisms are distributed worldwide with some being saprophytes and others being plant and animal parasites. Many species are of considerable economic importance due to their pathogenic effects on agriculture and livestock.
The ability to learn and to deal with new situations and to deal effectively with tasks involving abstractions.
Computer programs based on knowledge developed from consultation with experts on a problem, and the processing and/or formalizing of this knowledge using these programs in such a manner that the problems may be solved.

Purification and characterization of 2-deoxy-scyllo-inosose synthase derived from Bacillus circulans. A crucial carbocyclization enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics. (1/31)

The biosynthesis of 2-deoxystreptamine, the central aglycon of a major group of clinically important aminoglycoside antibiotics, commences with the initial carbocycle formation step from D-glucose-6-phosphate to 2-deoxy-scyllo-inosose. This crucial step is known to be catalyzed by 2-deoxy-scyllo-inosose synthase, which has not yet been characterized so far. Reported in this paper is the first purification of 2-deoxy-scyllo-inosose synthase from butirosin-producing Bacillus circulans SANK 72073 to electrophoretic homogeneity. The enzyme was isolated as a heterodimeric protein comprising from a 23 kDa- and a 42 kDa polypeptide chains. The Km of the enzyme for D-glucose-6-phosphate was estimated to be 9.0 x 10(-4) M and that for NAD+ 1.7 x 10(-4) M, kcat for D-glucose-6-phosphate being 7.3 x 10(-2) s(-1). The presence of Co2+ was essential for the enzyme activity, but Zn2+ was totally inhibitory. While the reaction mechanisms are quite similar, 2-deoxy-scyllo-inosose synthase appears to be distinct from dehydroquinate synthase in the shikimate pathway, with respect to the quaternary structure, metal ion requirement, and the kinetic parameters.  (+info)

Butirosin-biosynthetic gene cluster from Bacillus circulans. (2/31)

Butirosin is an interesting 2-deoxystreptamine (DOS)-containing aminoglycoside antibiotic produced by non-actinomycete Bacilli. Recently we were successful in purification of 2-deoxy-scyllo-inosose synthase from butirosin-producer Bacillus circulans as the key enzyme for the biosynthesis of DOS, in cloning of the responsible gene (btrC), and in its overexpression in Escherichia coli. The present study involved gene-walking approach, which allowed us to find a gene cluster around btrC. The function of each gene was further investigated by gene disruption, and the disruptants of btrB, btrC, btrD and btrM showed no antibiotic producing activity. Therefore, the gene cluster found so far was determined to be a part of the butirosin biosynthetic gene cluster. Functions of some ORFs are also discussed in terms of butirosin biosynthesis on the basis of database search.  (+info)

Significance of the 20-kDa subunit of heterodimeric 2-deoxy-scyllo-inosose synthase for the biosynthesis of butirosin antibiotics in Bacillus circulans. (3/31)

A gene (btrC2) encoding the 20-kDa subunit of 2-deoxy-scyllo-inosose (DOI) synthase, a key enzyme in the biosynthesis of 2-deoxystreptamine, was identified from the butirosin-producer Bacillus circulans by reverse genetics. The deduced amino acid sequence of BtrC2 closely resembled that of YaaE of B. subtilis, but the function of the latter has not been known to date. Instead, BtrC2 appeared to show sequence similarity to a certain extent with HisH of B. subtilis, an amidotransferase subunit of imidazole glycerol phosphate synthase. Disruption of btrC2 reduced the growth rate compared with the wild type, and simultaneously antibiotic producing activity was lost. Addition of NH4Cl to the medium complemented only the growth rate of the disruptant, and both the growth rate and antibiotic production were restored by addition of yeast extract. In addition, a heterologous co-expression system of btrC2 with btrC was constructed in Escherichia coli. The simultaneously over-expressed BtrC2 and BtrC constituted a heterodimer, the biochemical features of which resembled those of DOI synthase from B. circulans more than those of the recombinant homodimeric BtrC. Despite the similarity of BtrC2 to HisH the heterodimer showed neither aminotransfer nor amidotransfer activity for 2-deoxy-scyllo-inosose as a substrate. All the observations suggest that BtrC2 is involved not only in the secondary metabolism, but also in the primary metabolism in B. circulans. The function of BtrC2 in the butirosin biosynthesis appears to be indirect, and may be involved in stabilization of DOI synthase and in regulation of its enzyme activity.  (+info)

Identification of L-glutamine: 2-deoxy-scyllo-inosose aminotransferase required for the biosynthesis of butirosin in Bacillus circulans. (4/31)

Using inverse PCR, two new genes (btrN and btrS) were identified upstream of the putative glycosyltransferase gene btrM in the butirosin-biosynthetic btr gene cluster of Bacillus circulans. The upstream gene btrS showed significant homology with stsC of Streptomyces griseus, which encodes L-glutamine:scyllo-inosose aminotransferase in the biosynthesis of streptomycin. The function of BtrS was further confirmed by heterologous expression in Escherichia coli and chemical identification of the conversion of 2-deoxy-scyllo-inosose into 2-deoxy-scyllo-inosamine. The identification of BtrS as L-glutamine:2-deoxy-scyllo-inosose aminotransferase is the first report of the aminotransferase gene responsible for 2-deoxystreptamine biosynthesis.  (+info)

New antibiotic produced by bacteria, 5-beta-D-xylofuranosylneamine. (5/31)

A new aminoglycoside antibiotic was isolated from the fermentation broths of two strains of Bacillus species. The antibiotic is active against gram-positive and some gram-negative bacteria, and its antimicrobial spectrum is similar to that of ribostamycin. The chemical structure was determined to be 5-beta-d-xylofuranosylneamine, which is identical to the deacylated product obtained from butirosin A.  (+info)

Butirosin compared with gentamicin in vitro and in vivo. (6/31)

Butirosin (BTN) (P. W. K. Woo, G. L. Coffey, H. W. Dion, S. A. Fusari, and G. D. Senos, U. S. Patent 3,541,078, 1970) is a new aminoglycoside antibiotic notably active against opportunist bacterial species within Pseudomonas, Klebsiella, Enterobacter, Serratia, and Proteus. Numerous comparative tests were carried out with BTN and gentamicin (GTM) in vitro and in experimental infections in mice. BTN was more active in Mueller-Hinton broth than in agar, but its activity was lessened at acid pH or under anaerobiosis, as has been observed with other aminoglycosides. In standard agar diffusion tests, inhibition zones greater than 12 mm around 30-mug BTN disks generally denoted susceptibility, equivalent to minimal inhibitory concentrations [Formula: see text] 25 mug/ml. Cross-resistance between BTN and GTM occurred in a variable manner, with a number of bacterial strains strongly resistant to GTM being moderately susceptible to BTN. In mice, after a single subcutaneous injection, absorption of both antibiotics was rapid, with peak serum levels occurring in 15 min; this was followed by rapid elimination with estimated serum half-lives of about 20 min for each. After peroral administration of high doses in mice, there was no appreciable absorption of BTN. Several tests were carried out to compare BTN and GTM with respect to minimal inhibitory concentrations in vitro, acute subcutaneous median mouse protective doses, peak serum levels at such doses, and the therapeutic ratios derived from acute median protective and lethal doses. Although GTM usually proved to be more potent antibacterially on a weight basis, observations on BTN indicated a superior effectiveness in terms of therapeutic ratios.  (+info)

Biosynthesis of the unique amino acid side chain of butirosin: possible protective-group chemistry in an acyl carrier protein-mediated pathway. (7/31)

Butirosins A and B are naturally occurring aminoglycoside antibiotics that have a (2S)-4-amino-2-hydroxybutyrate (AHBA) side chain. Semisynthetic addition of AHBA to clinically valuable aminoglycoside antibiotics has been shown both to improve their pharmacological properties and to prevent their deactivation by a number of aminoglycoside-modifying enzymes involved in bacterial resistance. We report here that the biosynthesis of AHBA from L-glutamate, encoded within a previously identified butirosin biosynthetic gene cluster, proceeds via intermediates tethered to a specific acyl carrier protein (ACP). Five components of the pathway have been purified and characterized, including the ACP (BtrI), an ATP-dependent ligase (BtrJ), a pyridoxal phosphate-dependent decarboxylase (BtrK), and a two-component flavin-dependent monooxygenase system (BtrO and the previously unreported BtrV). The proposed biosynthetic pathway includes a gamma-glutamylation of an ACP-derived gamma-aminobutyrate intermediate, possibly a rare example of protective group chemistry in biosynthesis.  (+info)

The ribostamycin biosynthetic gene cluster in Streptomyces ribosidificus: comparison with butirosin biosynthesis. (8/31)

A cluster of genes for ribostamycin (Rbm) biosynthesis was isolated from Streptomyces ribosidificus ATCC 21294. Sequencing of 31.892 kb of the genomic DNA of S. ribosidificus revealed 26 open reading frames (ORFs) encoding putative Rbm biosynthetic genes as well as resistance and other genes. One of ten putative Rbm biosynthetic genes, rbmA, was expressed in S. lividans TK24, and shown to encode 2-deoxy-scyllo-inosose (DOI) synthase. Acetylation of various aminoglycoside-aminocyclitol (AmAcs) by RbmI confirmed it to be an aminoglycoside 3-N-acetyltransferase. Comparison of the genetic control of ribostamycin and butirosin biosynthesis pointed to a common biosynthetic route for these compounds, despite the considerable differences between them in genetic organization.  (+info)

These β-TrCP2|sup|f|/sup| β-TrCP1|sup|-|/sup| mice harbor two mutations; the β-TrCP2 floxed allele (|i|Fbxw11|sup|tm1.1Ybn|/sup||/i|) and the β-TrCP1 knockout allele (|i|Btrc|sup|tm1Paga|/sup||/i|). These mice are a Cre recombinase-inducible double knockout of β-TrCP function and allow study of multiple cellular processes (Wnt pathway, NF-κB pathway, cell cycle regulation, DNA damage response) and cancer.
Khaw, L.E., Bohm, G.A., Metcalfe, S., Staunton, J. and Leadlay, P.F. (1998). „Mutational biosynthesis of novel rapamycins by a strain of Streptomyces hygroscopicus NRRL 5491 disrupted in rapL, encoding a putative lysine cyclodeaminase. J. Bacteriol. 180: 809-814. PMID 9473033 ...
TY - JOUR. T1 - Crystal structure of β-galactosidase from Bacillus circulans ATCC 31382 (BgaD) and the construction of the thermophilic mutants (共著). AU - Nakabayashi, Makoto. PY - 2015. Y1 - 2015. M3 - Article. JO - FEBS J. 2015 Jul;282(13):2540-52.. JF - FEBS J. 2015 Jul;282(13):2540-52.. ER - ...
Neste trabalho foi desenvolvido um novo bioprocesso para a síntese de lactosacarose, um candidato a prebiótico. A lactosacarose foi produzida por transgalactosilação, catalisada pela β-galactosidase de Bacillus circulans imobilizada em macroesfera de quitosana, utilizando a lactose e a sacarose como substratos. No processo de imobilização, os resultados indicam que a melhor razão entre a concentração de enzima e de suporte foi de 200 mg.g-1. A estabilidade térmica da enzima imobilizada foi determinada e comparada com a estabilidade térmica da enzima livre em temperaturas de 50, 60 e 70 °C e para esta última foi verificada a estabilidade na presença e ausência de substrato. A imobilização aumentou de 10 a 260 vezes a estabilidade térmica da enzima, sendo este efeito inversamente relacionado com a temperatura. A otimização das condições de produção indica, para a β-galactosidase imobilizada e livre, que a melhor condição de produção de lactosacarose e de ...
IMMOBILIZATION AND PROPERTIES OF BACILLUS CIRCULANS LEVANSUCRASE HEBA A EL-REFAİ, AHMED F ABDEL-FATTAH, FATEN A MOSTAFA Abstract PDF Similar Articles ...
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Also acts on the antibiotics neomycin, paromomycin, neamine, paromamine, vistamycin and gentamicin A. An enzyme from Pseudomonas aeruginosa also acts on butirosin ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
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View Nf1/Nf1 Tg(Fabp7-cre,-lacZ)3Gtm/0 involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA: phenotypes, images, diseases, and references.
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Deoxyribonucleic acid reassociation measurements revealed that 50 of 111 Bacillus circulans Jordan 1890 strains examined could be separated into 10 groups of genetically related organisms. The results of deoxyribonucleic acid relatedness studies and phenotypic comparisons suggested that five of these groups were hitherto undescribed species and that four others probably represented previous ly, but inadequately, described species. The tenth group, which included the type strain, represented the species B. circulans sensu stricto. Our data show that B. circulans and Bacillus macerans Schardinger 1905 are genetically and phenotypically distinct species. Thus, the phenotypic heterogeneity of the species B. circulans sensu lato is not due to inherent variability of genetically related strains but rather is due to inclusion of genetically unrelated organisms in this taxon.
tRNA 2-phosphotransferase, catalyzes the final step in yeast tRNA splicing: the transfer of the 2-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1-2cyclic phosphate and ...
The 20 kDa Bacillus circulans Bcx is a well-studied endoxylanase with a beta-jellyroll fold that places its N- and C-termini in salt bridge contact. initial experiments verified that Bcx could be circularly permuted. by PCR methods to introduce new termini in loop regions while linking its native termini directly or via one or two glycines. Subsequently, a library Of circular permutants, generated by random DNase cleavage of the circularized Bcx gene, was screened for xylarlase activity oil xylan in Congo Red-stained agar. Analysis of 35 unique active circular permutants revealed that, while many of the new termini were introduced in external loops as anticipated, a surprising number were also located within beta-strands. Furthermore, several permutations placed key catalytic residucs at or near the new termini with minimal delecterious effects Oil activity and, in one case, a 4-fold increase. The structure of one permutant Was determined by X-ray crystallography, whereas three others were ...
Regulation and production of Fibrinolytic enzymes from bacterial sources especially from Bacillus strains has taken a leading role in the medical sciences for the treatment of cardiovascular disorders as it removes thrombus or clots adding to its significant role in curing human health issues saving millions. Significant progress has been made during the last few years on the studies of fibrinolytic enzymes in identifying, cloning, purification, characterization and overproduction of these for commercialization in medical sciences and in fields like detergents development. Production of fibrinolytic enzyme from Bacillus circulans was done using Nutrient broth medium. In addition, a strong fibrinolytic enzyme was purified from the cultivation media. The purified enzyme was almost homogeneous with other species of same genus, as examined by SDS−PAGE and sephadex G-75 column chromatography. The enzyme had an optimal pH of 7-12, an optimal temperature of 50 °C, for fibrin hydrolysis. The molecular mass
Expression of FBXW11 (BTRC2, BTRCP2, Fbw11, Fbw1b, FBXW1B, Hos, KIAA0696) in stomach 1 tissue. Antibody staining with HPA072204 in immunohistochemistry.
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The bacterium Legionella pneumophila is the responsible agent for Legionnaires disease and has recently been shown to harbor a gene encoding a kinase that confers resistance to the aminoglycoside antibiotic spectinomycin (Suter, T. M., Viswanathan, V. K., and Cianciotto, N. P. (1997) Antimicrob. Agents Chemother. 41, 1385-1388). We report the overproduction, purification, and characterization of this spectinomycin kinase from an expressing system in Escherichia coli. The purified protein shows stringent substrate specificity for spectinomycin with Km = 21.5 microM and kcat = 24.2 s-1 and does not bind other aminoglycosides including kanamycin, amikacin, neomycin, butirosin, streptomycin, or apramycin. Purification of spectinomycin phosphate followed by characterization by mass spectrometry and 1H, 13C, and 31P NMR established the site of phosphorylation to be at the hydroxyl group at position 9. Thus this enzyme is designated APH(9)-Ia (where APH is aminoglycoside kinase). The enzyme was inactivated by
Expression of FBXW11 (BTRC2, BTRCP2, Fbw11, Fbw1b, FBXW1B, Hos, KIAA0696) in heart muscle tissue. Antibody staining with HPA072204 in immunohistochemistry.
Two clinical isolates of Pseudomonas aeruginosa, one a pyocin type 5 strain from Atlanta, could transfer gentamicin resistance by conjugation. Donor and recipient strains inactivated gentamicin by acetylation. The R plasmids, pMG1 and pMG2, also determined resistance to sisomicin, another substrate of gentamicin acetyltransferase I, sulfonamides, and streptomycin, but not resistance to kanamycin, neomycin, tobramycin, butirosin, or BB-K 8. They were transmissible to many strains of P. aeruginosa, including a Rec− strain, but not to Escherichia coli or other enterobacteriaceae. These R plasmids were compatible with R plasmids transmissible to P. aeruginosa from E. coli, including members of C, N, P, and W incompatibility groups. From a strain carrying pMG1 and a compatible plasmid, pMG1 was transferred independently but transfer of the second plasmid often resulted in cotransfer of pMG1. In contrast, pMG1 and pMG2 were incompatible with pseudomonas R plasmids R931 and R3108, and with R931 they ...
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Hi Thomas,. Thanks for your reply - sorry I didnt see the email notifying me of your response sooner.. After my previous message I started my optimize experiments anyway, even though the diagnostics were failing.. My results appeared to come in like always - it seems to me that they are still working. Strictly speaking Im not sure because I cant see what my site visitors are seeing, but the results appear normal when I view the report in Google Optimize.. Just now I tried to follow your advice here. I went into the advanced section in the GTM4WP settings, ticked the box Load GTM container as early as possible, and hit save. I cleared saved files in my browser and re-ran the diagnostics a few times - but the diagnostics give the same errors as before ...
GTM Squareds annual membership is designed for energy leaders and enterprises. Professionals stay smart with Squareds intel platform that gives access to insights they want, when they want them. The membership includes:. Weekly Insights From Our ...
1CDG: Nucleotide sequence and X-ray structure of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 in a maltose-dependent crystal form.
Thank you for sharing this Journal of Bacteriology article.. NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.. ...
Wiki-Pi: a web resource for human protein-protein interactions. It shows genes and PPIs with information about pathways, protein-protein interactions (PPIs), Gene Ontology (GO) annotations including cellular localization, molecular function and biological process, drugs, diseases, genome-wide association studies (GWAS), GO enrichments, PDB ID, Uniprot ID, HPRD ID, and word cloud from pubmed abstracts.
Tn5 ATCC ® 87140™ Designation: pBSL97 TypeStrain=False Application: contains easily purifiable cassette(s) for construction aminoglycoside phosphotransferase kanamycin resistance, neomycin resistance
Just Chill Stress Relief Drink in Tropical - These drinks have SunTheanine, a patented form of L-theanine, which is a unique amino acid found in green tea that has been shown to help improve performance by reducing stress and increasing focus without causing drowsiness. Im usually pretty low-stress in my daily life, but I drank this at work while I was sick and it had a nice peachy flavor and I definitely felt relaxed. Im not sure if that was due to the fact that I usually dont get stressed in the first place, but it certainly didnt hurt ...
The aminoglycoside phosphotransferases (APHs) are responsible for the bacterial inactivation of many clinically useful aminoglycoside antibiotics. We report the characterization of an enterococcal enzyme, APH(3)-IIIa, which inactivates a broad spectrum of aminoglycosides by ATP-dependent O-phosphorylation. Overproduction of APH(3)-IIIa has permitted the isolation of 30-40 mg of pure protein/(L of cell culture). Purified APH(3)-IIIa is a mixture of monomer and dimer which is slowly converted to dimer only over time. Dimer could be dissociated into monomer by incubation with 2-mercaptoethanol, suggesting that dimerization is mediated by formation of disulfide bond(s). Both monomer and dimer show Km values in the low micromolar range for good substrates such as kanamycin and neomycin, and kcat values of 1-4 s-1. All aminoglycosides show substrate inhibition except amikacin and kanamycin B. Determination of minimum inhibitory concentrations indicates a positive correlation between antibiotic activity and
1OT1: The fully conserved Asp residue in Conserved sequence region I of the alpha-amylase Family is crucial for the Catalytic Site Architecture and Activity
The GTM Research Reserve offers an introduction to the Volunteer program for those who sign up. Learn about the background and history of the reserve, volunteer opportunities available to you, volunteer expectations and benefits, and tour the Environmental Education Center.. Orientation is usually held the first Friday of the month. For more information, contact volunteer coordinator Shannon Rininger at 904-823-4500.. ...
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Bacillus circulans subsp. circulans clone Bcir-1R 16S ribosomal RNA gene,partial sequence; 16S-23S internal transcribed spacer, complete sequence;and 23S ribosomal RNA gene, partial ...
In 1730, the Inquisition of Lisbon arrested José Francisco, an enslaved man raised in West Africa, who had learned in Brazil the art and craft of making amulets known as bolsas de mandinga. In their composition, use, and afterlives in the Inquisition the bolsas reveal the deep and mutually transformative spiritual and material connections that the slave trade engendered between Europeans and Africans in the early modern Atlantic World. Created and used in parallel to similar objects made elsewhere on the continent, once deemed fetishes and now considered central to the canon of African art, they waged a spirited battle against the witchcraft of the slave trade.. Cécile Fromont is an associate professor in the history of art department at Yale University. Her writing and teaching focus on the visual, material, and religious culture of Africa and Latin America with a special emphasis on the early modern period (ca 1500-1800) and on the Portuguese-speaking Atlantic World.. Her first book, The Art ...
... butirosin sulfate MeSH D09.408.051.374 - gentamicins MeSH D09.408.051.374.785 - sisomicin MeSH D09.408.051.374.785.525 - ... heparitin sulfate MeSH D09.698.373.425.500 - heparan sulfate proteoglycan MeSH D09.698.373.475 - hyaluronic acid MeSH D09.698. ... chondroitin sulfates MeSH D09.698.373.200.380 - dermatan sulfate MeSH D09.698.373.400 - heparin MeSH D09.698.373.400.300 - ... heparan sulfate proteoglycan MeSH D09.698.735.800 - platelet factor 4 MeSH D09.698.735.830 - proteochondroitin sulfates MeSH ...
"Kanamycin Sulfate". The American Society of Health-System Pharmacists. Archived from the original on 10 September 2017. ... The first part is common to several aminoglycoside antibiotics, such as butirosin and neomycin. In it a unique aminocyclitol, 2 ...
... chondroitin-sulfate-ABC endolyase EC 4.2.2.21: chondroitin-sulfate-ABC exolyase EC 4.2.2.22: pectate trisaccharide-lyase EC 4.2 ... gamma-L-glutamyl-butirosin B gamma-glutamyl cyclotransferase EC 4.3.3.1: 3-ketovalidoxylamine C-N-lyase EC 4.3.3.2: ... heparin-sulfate lyase EC 4.2.2.9: pectate disaccharide-lyase EC 4.2.2.10: pectin lyase EC 4.2.2.11: poly(a-L-guluronate) lyase ... serine-sulfate ammonia-lyase EC 4.3.1.11: dihydroxyphenylalanine ammonia-lyase EC 4.3.1.12: ornithine cyclodeaminase EC 4.3. ...
"Neomycin sulfate, EP Susceptibility and Minimum Inhibitory Concentration (MIC) Data" (PDF). TOKU-E.. ... Kudo F, Fujii T, Kinoshita S, Eguchi T (July 2007). "Unique O-ribosylation in the biosynthesis of butirosin". Bioorganic & ...
Hyperthermophilic sulfate-reducing archaen Archaeoglobus fulgidus has been recently reported to enable anaerobic oxidation of ... butirosin biosynthesis pathway oxidoreductase (aminoglycoside antibiotic biosynthesis) BzaF - 5-hydroxybenzimidazole (5-HBI) ... November 2014). "Anaerobic oxidation of long-chain n-alkanes by the hyperthermophilic sulfate-reducing archaeon, Archaeoglobus ...
... (List of chemicals that may cause contact dermatitis / Chemicalien die contacteczeem kunnen ... Butirosin Dihydrostreptomycin Framycetin Gentamycin Kanamycin Paromomycin Spectinomycin Tobramycin Unusual Reactions. Contact ...
Bispyrithione Magsulfex; Butikacin; Butirosin Sulfate; Capreomycin Sulfate; Carbadox; Carbenicillin Disodium; Carbenicillin ... Soluble lubricants may also be used such as sodium lauryl sulfate, magnesium lauryl sulfate, polyethylene glycol of various ... Trimethoprim Sulfate; Trisulfapyrimidines; Troleandomycin; Trospectomycin Sulfate; Tyrothricin; Vancomycin; Vancomycin ... 68 -sodium lauryl sulfate, dioctyl sodium sulfosuccinate and dioctyl sodium sulfonate. Cationic detergents might be used and ...
... butirosin sulfate MeSH D09.408.051.374 - gentamicins MeSH D09.408.051.374.785 - sisomicin MeSH D09.408.051.374.785.525 - ... heparitin sulfate MeSH D09.698.373.425.500 - heparan sulfate proteoglycan MeSH D09.698.373.475 - hyaluronic acid MeSH D09.698. ... chondroitin sulfates MeSH D09.698.373.200.380 - dermatan sulfate MeSH D09.698.373.400 - heparin MeSH D09.698.373.400.300 - ... heparan sulfate proteoglycan MeSH D09.698.735.800 - platelet factor 4 MeSH D09.698.735.830 - proteochondroitin sulfates MeSH ...
Various concentrations of kanamycin sulfate, gentamicin sulfate, tobramycin, dibekacin, neomycin sulfate, butirosin, and ... The eluted enzyme was determined to be homogeneous by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) ... which was supplemented with 30 μg of kanamycin sulfate per ml in the case of pET30a(+)-containing strains (Novagen). All ... presence of an ethyl group on the 1-amino group of the 2-deoxystreptamine ring is suggested by the data provided with butirosin ...
Butirosin A. Butirosin B. Butirosin Sulfate, Anhydrous. Butirosin Sulphate. Butirosins. Sulfate, Anhydrous Butirosin. Sulfate, ... Butirosin Sulfate Entry term(s). Butirosin Sulphate Sulfate, Butirosin Sulphate, Butirosin Butirosin Sulfate, Anhydrous - ... Butirosin Sulfate - Preferred Concept UI. M0003092. Scope note. A water-soluble aminoglycosidic antibiotic complex isolated ... Butirosin Sulfate, Anhydrous Entry term(s). Anhydrous Butirosin Sulfate Sulfate, Anhydrous Butirosin ...
... or butirosin sulfate? or calcimycin? or candicidin? or capreomycin? or carbenicillin? or carfecillin? or cefaclor? or ... or dihydrostreptomycin sulfate? or diketopiperazine? or distamycin? or doxycycline? or echinomycin? or edeine? or enviomycin? ...
Butirosin Sulfate (0) * Gentamicins (0) * Hygromycin B (0) * Kanamycin (4) * Amikacin (1) ...
butirosin sulfate*bacterial transformation*peptide termination factors*r factors*acriflavine*corynebacterium*amdinocillin* ... The function of BtrC2 in the butirosin biosynthesis appears to be indirect, and may be involved in stabilization of DOI ... Significance of the 20-kDa subunit of heterodimeric 2-deoxy-scyllo-inosose synthase for the biosynthesis of butirosin ...
Butirosin Sulfate. *Gentamicins. *Hygromycin B. *Kanamycin. *Metrizamide. *Neomycin. *Puromycin. *Spectinomycin. *Streptomycin ...
Butirosin and neomycin biosynthesis_Homo sapiens_hsa00524. 2.22389794. 9. Glycosaminoglycan biosynthesis - keratan sulfate_Homo ...
... dermatan sulfate Y inflammation proteoglycan dermatan sulfate tumors, X proteoglycans inflammation endothelin tumors, G ... butirosin, butixirate, butobendine, butoconazole, butocrolol, butoctamide, butofilolol, butonate, butopamine, butopiprine, ... capreomycin sulfate, cyclosexine, ethambutol, ethionamide, pyrazinamide, rifampin or streptomycin sulphate; antivirals such as ... 0340] DSPS (Avanti, 5.0 mg), lipopeptide from a)(0.3 mg)and polymixin B sulphate (Sigma, 0.5 mg) were weighed into a clean vial ...
Nickel sulfate hexahydrate; sulfuric acid, nickel (2+) salt, hexahydrate; nickel monosulfate hexahydrate; Blue salt; single ... butirosin, bacitracin. ICU. ...
After stirring for 1 hour, add the sodium sulfate followed by filtration. The sodium sulfate on the filter is washed with five ... The solution (100 ml), 35 g of 1-hydroxy-3-(3,4 - acid)butirosin during the night. The reaction mixture is extracted three ... The extract was dried over anhydrous sodium sulfate and the solvent is removed under reduced pressure to obtain a brown oily ... The extract was dried over anhydrous sodium sulfate and the solvent is removed under reduced pressure to obtain a pale brown ...
"Kanamycin Sulfate". The American Society of Health-System Pharmacists. Archived from the original on 10 September 2017. ... The first part is common to several aminoglycoside antibiotics, such as butirosin and neomycin. In it a unique aminocyclitol, 2 ...
Antibiotics such as polymyxin B sulfate-neomycin sulfate, cleocin phosphate ® (available from the Upjohn Company, Kalamazoo, ... Additional suitable antibiotics include aminoglycoside antibiotics such as amikacin, butirosin, dideoxykanamycin B (DKP), ...
EC 4.2.2.20 chondroitin-sulfate-ABC endolyase. EC 4.2.2.21 chondroitin-sulfate-ABC exolyase. EC 4.2.2.22 pectate trisaccharide- ... EC 4.3.2.6 γ-L-glutamyl-butirosin B γ-glutamyl cyclotransferase. EC 4.3.2.7 glutathione-specific γ-glutamylcyclotransferase EC ... EC 4.2.2.8 heparin-sulfate lyase. EC 4.2.2.9 pectate disaccharide-lyase. EC 4.2.2.10 pectin lyase. EC 4.2.2.11 guluronate- ... EC 4.3.1.10 serine-sulfate ammonia-lyase. EC 4.3.1.11 deleted. EC 4.3.1.12 ornithine cyclodeaminase. EC 4.3.1.13 carbamoyl- ...
Aluminum SucroseSulfate, AmmoniumSulfate, BariumSulfate, ButirosinSulfate, CalciumSulfate, ChondroitinSulfate, CopperSulfate, ... Sodium TetradecylSulfate, XylanSulfate, ZincSulfated Glycoprotein 2Sulfated Glycoprotein-2SulfatesSulfates, ChondroitinSulfates ... DermatanSulfate, DextranSulfate, DHASulfate, DHEASulfate, DihydrostreptomycinSulfate, GentamicinSulfate, HeparanSulfate, ... IndoxylSulfate, KeratanSulfate, MagnesiumSulfate, PolypentoseSulfate, PrasteroneSulfate, Sodium DodecylSulfate, Sodium Lauryl ...
"Neomycin sulfate, EP Susceptibility and Minimum Inhibitory Concentration (MIC) Data" (PDF). TOKU-E.. ... Kudo F, Fujii T, Kinoshita S, Eguchi T (July 2007). "Unique O-ribosylation in the biosynthesis of butirosin". Bioorganic & ...
Butirosin Sulfate; Capreomycin Sulfate; Carbadox; Carbenicillin Disodium; Carbenicillin Indanyl Sodium; Carbenicillin Phenyl ... vinblastine sulfate; vincristine sulfate; vindesine; vindesine sulfate; vinepidine sulfate; vinglycinate sulfate; vinleurosine ... sulfate; vinorelbine tartrate; vinrosidine sulfate; vinzolidine sulfate; vorozole; zeniplatin; zinostatin; zorubicin ... Neomycin Sulfate; Neomycin Undecylenate; Netilmicin Sulfate; Neutramycin; Nifuradene; Nifuraldezone; Nifuratel; Nifuratrone; ...
0188] (i) carboxylic acids, sulfates and phosphates (e.g. for reacting with a spacer precursor moiety comprising a primary ... Butirosin, Clindamycin, Clomocycline, Chloramphenicol, Cloxacillin, Colistin, Demeclocycline, Deoxydihydrostreptomycin, ... The filtrate was washed with 0.01M HCl solution (3×50 ml), dried over sodium sulfate, filtered and concentrated under reduced ... 0222] Amino-, Hydroxyl-, and Carboxyl-containing (including sulfate, phosphate and phosphonate-containing): p-Aminosalicylic ...
Serine-sulfate ammonia-lyase 4.3.1.11 Deleted entry 4.3.1.12 Ornithine cyclodeaminase 4.3.1.13 Carbamoyl-serine ammonia-lyase ... Gamma-L-glutamyl-butirosin B gamma-glutamyl cyclotransferase 4.3.2.7 Glutathione-specific gamma-glutamylcyclotransferase 4.3. ...
This locus encodes a glycosylated transmembrane receptor. Its ligand, autocrine motility factor, is a tumor motility-stimulating protein secreted by tumor cells. The encoded receptor is also a member of the E3 ubiquitin ligase family of proteins. It catal…
Butirosin Sulfate , Kanamycin , Tobramycin , Paromomycin , Aminoglycosides - chemical synthesis ... Butirosin Sulfate - biosynthesis , Kanamycin - biosynthesis , Anti-Bacterial Agents - biosynthesis , Cyclohexanols - ...
4-DIOL BUTIROSIN DISULFATE Streptomycin sulfate D-INS(1,4,5)P3, 3LI MYO-INOSITOL 2-MONOPHOSPHATE DI(CYCLOHEXYLAMMONIUM) SALT 1L ... 2-INOSOSE MYO-INOSITOL HEXASULFATE HEXAPOTASSIUM SALT FORSKOLIN, 7-DEACETYL-7-O-HEMISUCCINYL- Neomycin sulfate Spectinomycin D- ...
Butirosin Sulfate - pharmacology , Escherichia coli - drug effects , Humans , Kanamycin - pharmacology , Stenotrophomonas ...
Butirosin B has been identified as1-N-[(S)-4-amino-2-hydroxy-n-butyryl]-ribostamycin; see the "Tetrahedron Letters" Vol. 28, ... sulfate, phosphate, acetate, maleate, fumarate, succinate,tartrate, oxalate, citrate, methanesulfonate, ethanesulfonate and the ... On the other hand, it is known that butirosin B which is an aminoglycosidic antibiotic produced by a Bacillus species is active ... 4-amino-2-hydroxybutyryl substituent on the 1-amino group of the butirosin B molecule has an important role in enabling the ...
ribostamycin sulfate salt (antibiotic) Increases from 1 studies. aminoglycosides antibiotics (prescription) sisomicin sulfate ( ... butirosin Increases from 1 studies. Antibiotics, Antivirals etc carbapenem (antibiotic)s Increases from 1 studies. ... sisomicin sulfate (antibiotic) Increases from 1 studies. Antibiotics, Antivirals etc spectinomycin (antibiotic)s Increases from ... ribostamycin sulfate salt (antibiotic) Increases from 1 studies. Antibiotics, Antivirals etc roxithromycin (antibiotic)s ...
Kanamycin Sulfate \ 101-25389-94-0 for more molecular products just contact us ... aphA4 aph butA] Aminoglycoside 3-phosphotransferase (EC 2.7.1.95) (APH(3)IV) (Butirosin resistance protein A) (Kanamycin ... We have also other products like : Kanamycin Sulfate Related products : Kanamycin Sulfate ... WP579: Sulfate assimilation pathway II. Related Genes :. [kamB] 16S rRNA (adenine(1408)-N(1))-methyltransferase (EC 2.1.1.180 ...
... butirosin, macrolactin, bacillaene, fengycin, lanthipeptides and LCI, were predicted in the genome of Bacillus sp. GFP-2, which ... 2016). Butirosin, an aminoglycoside antimicrobial peptide, is known as the important class of agents, especially against ... GFP-2 were extracted by ammonium sulfate precipitation method from the culture supernatant. Then 200 μl of Na3PO4 buffer (a), ... GFP-2 were extracted by ammonium sulfate precipitation method and the glucan hydrolysis assay was performed. As shown in Fig. 4 ...
... sulfate de magnésium laurilyque myristate de magnésium oxide de magnésium silicate magnésium stéarate de magnésium sulfate de ... butirosin, butixirate, butixocort, butizide, butobendine, butoconazole, butocrolol, butoctamide, butofilolol, butonate, ... dammonium carbonate dammonium glycyrrhisate dammonium hydroxyde dammonium sulfate de laureth et dammonium sulfate de ... copolymer ammonium carbonate ammonium glycyrrhizate ammonium hydroxide ammonium laureth sulfate ammonium lauryl sulfate ...
  • Neomycin sulfate is a widely used antibiotic. (huidziekten.nl)
  • The first part is common to several aminoglycoside antibiotics, such as butirosin and neomycin. (wikipedia.org)
  • As the aminoglycosidic antibiotics containing a neamine moiety in the molecule thereof are known neamine (that is, neomycin A), neomycin B, neomycin C, ribostamycin (known also as vistamycin), xylostatin, butirosin A, butirosin B and kanamycin B. From Belgian Pat. (google.com)