Butirosin Sulfate: A water-soluble aminoglycosidic antibiotic complex isolated from fermentation filtrates of Bacillus circulans. Two components (A and B) have been separated from the complex. Both are active against many gram-positive and some gram-negative bacteria.Ribostamycin: A broad-spectrum antimicrobial isolated from Streptomyces ribosifidicus.Framycetin: A component of NEOMYCIN that is produced by Streptomyces fradiae. On hydrolysis it yields neamine and neobiosamine B. (From Merck Index, 11th ed)Aminoglycosides: Glycosylated compounds in which there is an amino substituent on the glycoside. Some of them are clinically important ANTIBIOTICS.Bacillus: A genus of BACILLACEAE that are spore-forming, rod-shaped cells. Most species are saprophytic soil forms with only a few species being pathogenic.Kanamycin: Antibiotic complex produced by Streptomyces kanamyceticus from Japanese soil. Comprises 3 components: kanamycin A, the major component, and kanamycins B and C, the minor components.Heparitin Sulfate: A heteropolysaccharide that is similar in structure to HEPARIN. It accumulates in individuals with MUCOPOLYSACCHARIDOSIS.Kanamycin Kinase: A class of enzymes that inactivate aminocyclitol-aminoglycoside antibiotics (AMINOGLYCOSIDES) by regiospecific PHOSPHORYLATION of the 3' and/or 5' hydroxyl.Anti-Bacterial Agents: Substances that reduce the growth or reproduction of BACTERIA.Neomycin: Antibiotic complex produced by Streptomyces fradiae. It is composed of neomycins A, B, and C. It acts by inhibiting translation during protein synthesis.Hepatic Encephalopathy: A syndrome characterized by central nervous system dysfunction in association with LIVER FAILURE, including portal-systemic shunts. Clinical features include lethargy and CONFUSION (frequently progressing to COMA); ASTERIXIS; NYSTAGMUS, PATHOLOGIC; brisk oculovestibular reflexes; decorticate and decerebrate posturing; MUSCLE SPASTICITY; and bilateral extensor plantar reflexes (see REFLEX, BABINSKI). ELECTROENCEPHALOGRAPHY may demonstrate triphasic waves. (From Adams et al., Principles of Neurology, 6th ed, pp1117-20; Plum & Posner, Diagnosis of Stupor and Coma, 3rd ed, p222-5)Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Streptomycin: An antibiotic produced by the soil actinomycete Streptomyces griseus. It acts by inhibiting the initiation and elongation processes during protein synthesis.Blind Loop Syndrome: A malabsorption syndrome that is associated with a blind loop in the upper SMALL INTESTINE that is characterized by the lack of peristaltic movement, stasis of INTESTINAL CONTENTS, and the overgrowth of BACTERIA. Such bacterial overgrowth interferes with BILE SALTS action, FATTY ACIDS processing, MICROVILLI integrity, and the ABSORPTION of nutrients such as VITAMIN B12 and FOLIC ACID.Breath Tests: Any tests done on exhaled air.Ammonia: A colorless alkaline gas. It is formed in the body during decomposition of organic materials during a large number of metabolically important reactions. Note that the aqueous form of ammonia is referred to as AMMONIUM HYDROXIDE.Terminology as Topic: The terms, expressions, designations, or symbols used in a particular science, discipline, or specialized subject area.Enzymes: Biological molecules that possess catalytic activity. They may occur naturally or be synthetically created. Enzymes are usually proteins, however CATALYTIC RNA and CATALYTIC DNA molecules have also been identified.International Agencies: International organizations which provide health-related or other cooperative services.Molecular Biology: A discipline concerned with studying biological phenomena in terms of the chemical and physical interactions of molecules.Databases, Genetic: Databases devoted to knowledge about specific genes and gene products.Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.International Cooperation: The interaction of persons or groups of persons representing various nations in the pursuit of a common goal or interest.alpha-Macroglobulins: Glycoproteins with a molecular weight of approximately 620,000 to 680,000. Precipitation by electrophoresis is in the alpha region. They include alpha 1-macroglobulins and alpha 2-macroglobulins. These proteins exhibit trypsin-, chymotrypsin-, thrombin-, and plasmin-binding activity and function as hormonal transporters.Patents as Topic: Exclusive legal rights or privileges applied to inventions, plants, etc.Inventions: A novel composition, device, or process, independently conceived de novo or derived from a pre-existing model.Intellectual Property: Property, such as patents, trademarks, and copyright, that results from creative effort. The Patent and Copyright Clause (Art. 1, Sec. 8, cl. 8) of the United States Constitution provides for promoting the progress of science and useful arts by securing for limited times to authors and inventors, the exclusive right to their respective writings and discoveries. (From Black's Law Dictionary, 5th ed, p1014)History, 18th Century: Time period from 1701 through 1800 of the common era.Biotechnology: Body of knowledge related to the use of organisms, cells or cell-derived constituents for the purpose of developing products which are technically, scientifically and clinically useful. Alteration of biologic function at the molecular level (i.e., GENETIC ENGINEERING) is a central focus; laboratory methods used include TRANSFECTION and CLONING technologies, sequence and structure analysis algorithms, computer databases, and gene and protein structure function analysis and prediction.History, 17th Century: Time period from 1601 through 1700 of the common era.Biomedical Research: Research that involves the application of the natural sciences, especially biology and physiology, to medicine.Ontario: A province of Canada lying between the provinces of Manitoba and Quebec. Its capital is Toronto. It takes its name from Lake Ontario which is said to represent the Iroquois oniatariio, beautiful lake. (From Webster's New Geographical Dictionary, 1988, p892 & Room, Brewer's Dictionary of Names, 1992, p391)Gene Library: A large collection of DNA fragments cloned (CLONING, MOLECULAR) from a given organism, tissue, organ, or cell type. It may contain complete genomic sequences (GENOMIC LIBRARY) or complementary DNA sequences, the latter being formed from messenger RNA and lacking intron sequences.Gene Expression Profiling: The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.Dentin-Bonding Agents: Cements that act through infiltration and polymerization within the dentinal matrix and are used for dental restoration. They can be adhesive resins themselves, adhesion-promoting monomers, or polymerization initiators that act in concert with other agents to form a dentin-bonding system.Parenteral Nutrition Solutions: Specialized solutions for PARENTERAL NUTRITION. They may contain a variety of MICRONUTRIENTS; VITAMINS; AMINO ACIDS; CARBOHYDRATES; LIPIDS; and SALTS.Research: Critical and exhaustive investigation or experimentation, having for its aim the discovery of new facts and their correct interpretation, the revision of accepted conclusions, theories, or laws in the light of newly discovered facts, or the practical application of such new or revised conclusions, theories, or laws. (Webster, 3d ed)Kanamycin Resistance: Nonsusceptibility of bacteria to the antibiotic KANAMYCIN, which can bind to their 70S ribosomes and cause misreading of messenger RNA.Drug Discovery: The process of finding chemicals for potential therapeutic use.Small Molecule Libraries: Large collections of small molecules (molecular weight about 600 or less), of similar or diverse nature which are used for high-throughput screening analysis of the gene function, protein interaction, cellular processing, biochemical pathways, or other chemical interactions.Gram-Positive Bacteria: Bacteria which retain the crystal violet stain when treated by Gram's method.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Capreomycin: Cyclic peptide antibiotic similar to VIOMYCIN. It is produced by Streptomyces capreolus.Dermatan Sulfate: A naturally occurring glycosaminoglycan found mostly in the skin and in connective tissue. It differs from CHONDROITIN SULFATE A (see CHONDROITIN SULFATES) by containing IDURONIC ACID in place of glucuronic acid, its epimer, at carbon atom 5. (from Merck, 12th ed)Absorbable Implants: Implants constructed of materials designed to be absorbed by the body without producing an immune response. They are usually composed of plastics and are frequently used in orthopedics and orthodontics.Collagen: A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).Thiocapsa: A genus of anoxygenic, photosynthetic, nonmotile, spherical to slightly ovoid bacterial cells occurring singly, or in aggregates of two or four, and usually surrounded with slime. It is found in stagnant water, mud of ponds, estuaries, and microbial mats of salt marshes. (From Bergey's Manual of Determinative Bacteriology, 9th ed)Polyesters: Polymers of organic acids and alcohols, with ester linkages--usually polyethylene terephthalate; can be cured into hard plastic, films or tapes, or fibers which can be woven into fabrics, meshes or velours.Chromatium: A genus of gram-negative, ovoid to rod-shaped bacteria that is phototrophic. All species use ammonia as a nitrogen source. Some strains are found only in sulfide-containing freshwater habitats exposed to light while others may occur in marine, estuarine, and freshwater environments.ElastinBooksConsultants: Individuals referred to for expert or professional advice or services.Culdoscopy: Endoscopic examination, therapy or surgery of the female pelvic viscera by means of an endoscope introduced into the pelvic cavity through the posterior vaginal fornix.Drug Contamination: The presence of organisms, or any foreign material that makes a drug preparation impure.Ambroxol: A metabolite of BROMHEXINE that stimulates mucociliary action and clears the air passages in the respiratory tract. It is usually administered as the hydrochloride.Dosage Forms: Completed forms of the pharmaceutical preparation in which prescribed doses of medication are included. They are designed to resist action by gastric fluids, prevent vomiting and nausea, reduce or alleviate the undesirable taste and smells associated with oral administration, achieve a high concentration of drug at target site, or produce a delayed or long-acting drug effect.

Purification and characterization of 2-deoxy-scyllo-inosose synthase derived from Bacillus circulans. A crucial carbocyclization enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics. (1/31)

The biosynthesis of 2-deoxystreptamine, the central aglycon of a major group of clinically important aminoglycoside antibiotics, commences with the initial carbocycle formation step from D-glucose-6-phosphate to 2-deoxy-scyllo-inosose. This crucial step is known to be catalyzed by 2-deoxy-scyllo-inosose synthase, which has not yet been characterized so far. Reported in this paper is the first purification of 2-deoxy-scyllo-inosose synthase from butirosin-producing Bacillus circulans SANK 72073 to electrophoretic homogeneity. The enzyme was isolated as a heterodimeric protein comprising from a 23 kDa- and a 42 kDa polypeptide chains. The Km of the enzyme for D-glucose-6-phosphate was estimated to be 9.0 x 10(-4) M and that for NAD+ 1.7 x 10(-4) M, kcat for D-glucose-6-phosphate being 7.3 x 10(-2) s(-1). The presence of Co2+ was essential for the enzyme activity, but Zn2+ was totally inhibitory. While the reaction mechanisms are quite similar, 2-deoxy-scyllo-inosose synthase appears to be distinct from dehydroquinate synthase in the shikimate pathway, with respect to the quaternary structure, metal ion requirement, and the kinetic parameters.  (+info)

Butirosin-biosynthetic gene cluster from Bacillus circulans. (2/31)

Butirosin is an interesting 2-deoxystreptamine (DOS)-containing aminoglycoside antibiotic produced by non-actinomycete Bacilli. Recently we were successful in purification of 2-deoxy-scyllo-inosose synthase from butirosin-producer Bacillus circulans as the key enzyme for the biosynthesis of DOS, in cloning of the responsible gene (btrC), and in its overexpression in Escherichia coli. The present study involved gene-walking approach, which allowed us to find a gene cluster around btrC. The function of each gene was further investigated by gene disruption, and the disruptants of btrB, btrC, btrD and btrM showed no antibiotic producing activity. Therefore, the gene cluster found so far was determined to be a part of the butirosin biosynthetic gene cluster. Functions of some ORFs are also discussed in terms of butirosin biosynthesis on the basis of database search.  (+info)

Significance of the 20-kDa subunit of heterodimeric 2-deoxy-scyllo-inosose synthase for the biosynthesis of butirosin antibiotics in Bacillus circulans. (3/31)

A gene (btrC2) encoding the 20-kDa subunit of 2-deoxy-scyllo-inosose (DOI) synthase, a key enzyme in the biosynthesis of 2-deoxystreptamine, was identified from the butirosin-producer Bacillus circulans by reverse genetics. The deduced amino acid sequence of BtrC2 closely resembled that of YaaE of B. subtilis, but the function of the latter has not been known to date. Instead, BtrC2 appeared to show sequence similarity to a certain extent with HisH of B. subtilis, an amidotransferase subunit of imidazole glycerol phosphate synthase. Disruption of btrC2 reduced the growth rate compared with the wild type, and simultaneously antibiotic producing activity was lost. Addition of NH4Cl to the medium complemented only the growth rate of the disruptant, and both the growth rate and antibiotic production were restored by addition of yeast extract. In addition, a heterologous co-expression system of btrC2 with btrC was constructed in Escherichia coli. The simultaneously over-expressed BtrC2 and BtrC constituted a heterodimer, the biochemical features of which resembled those of DOI synthase from B. circulans more than those of the recombinant homodimeric BtrC. Despite the similarity of BtrC2 to HisH the heterodimer showed neither aminotransfer nor amidotransfer activity for 2-deoxy-scyllo-inosose as a substrate. All the observations suggest that BtrC2 is involved not only in the secondary metabolism, but also in the primary metabolism in B. circulans. The function of BtrC2 in the butirosin biosynthesis appears to be indirect, and may be involved in stabilization of DOI synthase and in regulation of its enzyme activity.  (+info)

Identification of L-glutamine: 2-deoxy-scyllo-inosose aminotransferase required for the biosynthesis of butirosin in Bacillus circulans. (4/31)

Using inverse PCR, two new genes (btrN and btrS) were identified upstream of the putative glycosyltransferase gene btrM in the butirosin-biosynthetic btr gene cluster of Bacillus circulans. The upstream gene btrS showed significant homology with stsC of Streptomyces griseus, which encodes L-glutamine:scyllo-inosose aminotransferase in the biosynthesis of streptomycin. The function of BtrS was further confirmed by heterologous expression in Escherichia coli and chemical identification of the conversion of 2-deoxy-scyllo-inosose into 2-deoxy-scyllo-inosamine. The identification of BtrS as L-glutamine:2-deoxy-scyllo-inosose aminotransferase is the first report of the aminotransferase gene responsible for 2-deoxystreptamine biosynthesis.  (+info)

New antibiotic produced by bacteria, 5-beta-D-xylofuranosylneamine. (5/31)

A new aminoglycoside antibiotic was isolated from the fermentation broths of two strains of Bacillus species. The antibiotic is active against gram-positive and some gram-negative bacteria, and its antimicrobial spectrum is similar to that of ribostamycin. The chemical structure was determined to be 5-beta-d-xylofuranosylneamine, which is identical to the deacylated product obtained from butirosin A.  (+info)

Butirosin compared with gentamicin in vitro and in vivo. (6/31)

Butirosin (BTN) (P. W. K. Woo, G. L. Coffey, H. W. Dion, S. A. Fusari, and G. D. Senos, U. S. Patent 3,541,078, 1970) is a new aminoglycoside antibiotic notably active against opportunist bacterial species within Pseudomonas, Klebsiella, Enterobacter, Serratia, and Proteus. Numerous comparative tests were carried out with BTN and gentamicin (GTM) in vitro and in experimental infections in mice. BTN was more active in Mueller-Hinton broth than in agar, but its activity was lessened at acid pH or under anaerobiosis, as has been observed with other aminoglycosides. In standard agar diffusion tests, inhibition zones greater than 12 mm around 30-mug BTN disks generally denoted susceptibility, equivalent to minimal inhibitory concentrations [Formula: see text] 25 mug/ml. Cross-resistance between BTN and GTM occurred in a variable manner, with a number of bacterial strains strongly resistant to GTM being moderately susceptible to BTN. In mice, after a single subcutaneous injection, absorption of both antibiotics was rapid, with peak serum levels occurring in 15 min; this was followed by rapid elimination with estimated serum half-lives of about 20 min for each. After peroral administration of high doses in mice, there was no appreciable absorption of BTN. Several tests were carried out to compare BTN and GTM with respect to minimal inhibitory concentrations in vitro, acute subcutaneous median mouse protective doses, peak serum levels at such doses, and the therapeutic ratios derived from acute median protective and lethal doses. Although GTM usually proved to be more potent antibacterially on a weight basis, observations on BTN indicated a superior effectiveness in terms of therapeutic ratios.  (+info)

Biosynthesis of the unique amino acid side chain of butirosin: possible protective-group chemistry in an acyl carrier protein-mediated pathway. (7/31)

Butirosins A and B are naturally occurring aminoglycoside antibiotics that have a (2S)-4-amino-2-hydroxybutyrate (AHBA) side chain. Semisynthetic addition of AHBA to clinically valuable aminoglycoside antibiotics has been shown both to improve their pharmacological properties and to prevent their deactivation by a number of aminoglycoside-modifying enzymes involved in bacterial resistance. We report here that the biosynthesis of AHBA from L-glutamate, encoded within a previously identified butirosin biosynthetic gene cluster, proceeds via intermediates tethered to a specific acyl carrier protein (ACP). Five components of the pathway have been purified and characterized, including the ACP (BtrI), an ATP-dependent ligase (BtrJ), a pyridoxal phosphate-dependent decarboxylase (BtrK), and a two-component flavin-dependent monooxygenase system (BtrO and the previously unreported BtrV). The proposed biosynthetic pathway includes a gamma-glutamylation of an ACP-derived gamma-aminobutyrate intermediate, possibly a rare example of protective group chemistry in biosynthesis.  (+info)

The ribostamycin biosynthetic gene cluster in Streptomyces ribosidificus: comparison with butirosin biosynthesis. (8/31)

A cluster of genes for ribostamycin (Rbm) biosynthesis was isolated from Streptomyces ribosidificus ATCC 21294. Sequencing of 31.892 kb of the genomic DNA of S. ribosidificus revealed 26 open reading frames (ORFs) encoding putative Rbm biosynthetic genes as well as resistance and other genes. One of ten putative Rbm biosynthetic genes, rbmA, was expressed in S. lividans TK24, and shown to encode 2-deoxy-scyllo-inosose (DOI) synthase. Acetylation of various aminoglycoside-aminocyclitol (AmAcs) by RbmI confirmed it to be an aminoglycoside 3-N-acetyltransferase. Comparison of the genetic control of ribostamycin and butirosin biosynthesis pointed to a common biosynthetic route for these compounds, despite the considerable differences between them in genetic organization.  (+info)

Khaw, L.E., Bohm, G.A., Metcalfe, S., Staunton, J. and Leadlay, P.F. (1998). „Mutational biosynthesis of novel rapamycins by a strain of Streptomyces hygroscopicus NRRL 5491 disrupted in rapL, encoding a putative lysine cyclodeaminase". J. Bacteriol. 180: 809-814. PMID 9473033 ...
Neste trabalho foi desenvolvido um novo bioprocesso para a síntese de lactosacarose, um candidato a prebiótico. A lactosacarose foi produzida por transgalactosilação, catalisada pela β-galactosidase de Bacillus circulans imobilizada em macroesfera de quitosana, utilizando a lactose e a sacarose como substratos. No processo de imobilização, os resultados indicam que a melhor razão entre a concentração de enzima e de suporte foi de 200 mg.g-1. A estabilidade térmica da enzima imobilizada foi determinada e comparada com a estabilidade térmica da enzima livre em temperaturas de 50, 60 e 70 °C e para esta última foi verificada a estabilidade na presença e ausência de substrato. A imobilização aumentou de 10 a 260 vezes a estabilidade térmica da enzima, sendo este efeito inversamente relacionado com a temperatura. A otimização das condições de produção indica, para a β-galactosidase imobilizada e livre, que a melhor condição de produção de lactosacarose e de ...
GNN - Genome News Network - The leading source of news about genomics research worldwide. Written for a general audience, GNN covers genomics and human medicine, the environment, stem cells, cloning, biotechnology, and agriculture.
Also acts on the antibiotics neomycin, paromomycin, neamine, paromamine, vistamycin and gentamicin A. An enzyme from Pseudomonas aeruginosa also acts on butirosin ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
View Nf1/Nf1 Tg(Fabp7-cre,-lacZ)3Gtm/0 involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA: phenotypes, images, diseases, and references.
National : ** Quality, time-befitting education a must: PM ** 4,400 families in Coxs Bazar to get homes under Ashrayan Project** Ex-Rangpur mayor Sharfuddin Ahmed Jhantu passes away ** BTRC opens new short-code 100 to hear complaint** ...
National : ** Quality, time-befitting education a must: PM ** 4,400 families in Coxs Bazar to get homes under Ashrayan Project** Ex-Rangpur mayor Sharfuddin Ahmed Jhantu passes away ** BTRC opens new short-code 100 to hear complaint** ...
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tRNA 2-phosphotransferase, catalyzes the final step in yeast tRNA splicing: the transfer of the 2-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1-2cyclic phosphate and ...
The 20 kDa Bacillus circulans Bcx is a well-studied endoxylanase with a beta-jellyroll fold that places its N- and C-termini in salt bridge contact. initial experiments verified that Bcx could be circularly permuted. by PCR methods to introduce new termini in loop regions while linking its native termini directly or via one or two glycines. Subsequently, a library Of circular permutants, generated by random DNase cleavage of the circularized Bcx gene, was screened for xylarlase activity oil xylan in Congo Red-stained agar. Analysis of 35 unique active circular permutants revealed that, while many of the new termini were introduced in external loops as anticipated, a surprising number were also located within beta-strands. Furthermore, several permutations placed key catalytic residucs at or near the new termini with minimal delecterious effects Oil activity and, in one case, a 4-fold increase. The structure of one permutant Was determined by X-ray crystallography, whereas three others were ...
Regulation and production of Fibrinolytic enzymes from bacterial sources especially from Bacillus strains has taken a leading role in the medical sciences for the treatment of cardiovascular disorders as it removes thrombus or clots adding to its significant role in curing human health issues saving millions. Significant progress has been made during the last few years on the studies of fibrinolytic enzymes in identifying, cloning, purification, characterization and overproduction of these for commercialization in medical sciences and in fields like detergents development. Production of fibrinolytic enzyme from Bacillus circulans was done using Nutrient broth medium. In addition, a strong fibrinolytic enzyme was purified from the cultivation media. The purified enzyme was almost homogeneous with other species of same genus, as examined by SDS−PAGE and sephadex G-75 column chromatography. The enzyme had an optimal pH of 7-12, an optimal temperature of 50 °C, for fibrin hydrolysis. The molecular mass
Expression of FBXW11 (BTRC2, BTRCP2, Fbw11, Fbw1b, FBXW1B, Hos, KIAA0696) in stomach 1 tissue. Antibody staining with HPA072204 in immunohistochemistry.
Expression of FBXW11 (BTRC2, BTRCP2, Fbw11, Fbw1b, FBXW1B, Hos, KIAA0696) in heart muscle tissue. Antibody staining with HPA072204 in immunohistochemistry.
Ormus Minerals for Natural Nutritional Energy and how it can bless your life and health. What is Ormus? Well it is something that is all around us in the Air, Water, Land and the food we eat. Now with todays technology we have learned how to use it as a Energy Nutrition supplement to help our lives be fuller of Life.
Hi Thomas,. Thanks for your reply - sorry I didnt see the email notifying me of your response sooner.. After my previous message I started my optimize experiments anyway, even though the diagnostics were failing.. My results appeared to come in like always - it seems to me that they are still working. Strictly speaking Im not sure because I cant see what my site visitors are seeing, but the results appear normal when I view the report in Google Optimize.. Just now I tried to follow your advice here. I went into the advanced section in the GTM4WP settings, ticked the box "Load GTM container as early as possible", and hit save. I cleared saved files in my browser and re-ran the diagnostics a few times - but the diagnostics give the same errors as before ...
1CDG: Nucleotide sequence and X-ray structure of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 in a maltose-dependent crystal form.
Thank you for sharing this Journal of Bacteriology article.. NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.. ...
... Designation: pBSL97 TypeStrain=False Application: contains easily purifiable cassette(s) for construction aminoglycoside phosphotransferase kanamycin resistance, neomycin resistance
Just Chill Stress Relief Drink in Tropical - These drinks have SunTheanine, a patented form of L-theanine, which is a unique amino acid found in green tea that has been shown to help improve performance by reducing stress and increasing focus without causing drowsiness. Im usually pretty low-stress in my daily life, but I drank this at work while I was sick and it had a nice peachy flavor and I definitely felt relaxed. Im not sure if that was due to the fact that I usually dont get stressed in the first place, but it certainly didnt hurt ...
1OT1: The fully conserved Asp residue in Conserved sequence region I of the alpha-amylase Family is crucial for the Catalytic Site Architecture and Activity
The GTM Research Reserve offers an introduction to the Volunteer program for those who sign up. Learn about the background and history of the reserve, volunteer opportunities available to you, volunteer expectations and benefits, and tour the Environmental Education Center.. Orientation is usually held the first Friday of the month. For more information, contact volunteer coordinator Shannon Rininger at 904-823-4500.. ...
Close The Infona portal uses cookies, i.e. strings of text saved by a browser on the users device. The portal can access those files and use them to remember the users data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser. ...
Bacillus circulans subsp. circulans clone Bcir-1R 16S ribosomal RNA gene,partial sequence; 16S-23S internal transcribed spacer, complete sequence;and 23S ribosomal RNA gene, partial ...
... butirosin sulfate MeSH D09.408.051.374 --- gentamicins MeSH D09.408.051.374.785 --- sisomicin MeSH D09.408.051.374.785.525 --- ... heparan sulfate proteoglycan MeSH D09.698.373.475 --- hyaluronic acid MeSH D09.698.373.550 --- keratan sulfate MeSH D09.698. ... chondroitin sulfates MeSH D09.698.373.200.380 --- dermatan sulfate MeSH D09.698.373.400 --- heparin MeSH D09.698.373.400.300 ... heparan sulfate proteoglycan MeSH D09.698.735.800 --- platelet factor 4 MeSH D09.698.735.830 --- proteochondroitin sulfates ...
"Neomycin sulfate, EP Susceptibility and Minimum Inhibitory Concentration (MIC) Data" (PDF). TOKU-E. Cammack, R. Attwood, T. K. ... "Unique O-ribosylation in the biosynthesis of butirosin". Bioorganic & Medicinal Chemistry. 15 (13): 4360-8. doi:10.1016/j.bmc. ...
"Kanamycin Sulfate". The American Society of Health-System Pharmacists. Archived from the original on 10 September 2017. ... The first part is common to several aminoglycoside antibiotics, such as butirosin and neomycin. In it a unique aminocyclitol, 2 ... "Kanamycin Sulfate". International Drug Price Indicator Guide. Retrieved 7 December 2016. "Kanamycin (By injection)". Archived ...
... chondroitin-sulfate-ABC endolyase EC 4.2.2.21: chondroitin-sulfate-ABC exolyase EC 4.2.2.22: pectate trisaccharide-lyase EC 4.2 ... gamma-L-glutamyl-butirosin B gamma-glutamyl cyclotransferase EC 4.3.3.1: 3-ketovalidoxylamine C-N-lyase EC 4.3.3.2: ... heparin-sulfate lyase EC 4.2.2.9: pectate disaccharide-lyase EC 4.2.2.10: pectin lyase EC 4.2.2.11: poly(a-L-guluronate) lyase ... serine-sulfate ammonia-lyase EC 4.3.1.11: dihydroxyphenylalanine ammonia-lyase EC 4.3.1.12: ornithine cyclodeaminase EC 4.3. ...
... (List of chemicals that may cause contact dermatitis / Chemicalien die contacteczeem kunnen ... Butirosin Dihydrostreptomycin Framycetin Gentamycin Kanamycin Paromomycin Spectinomycin Tobramycin Unusual Reactions. Contact ...
Bispyrithione Magsulfex; Butikacin; Butirosin Sulfate; Capreomycin Sulfate; Carbadox; Carbenicillin Disodium; Carbenicillin ... Soluble lubricants may also be used such as sodium lauryl sulfate, magnesium lauryl sulfate, polyethylene glycol of various ... Trimethoprim Sulfate; Trisulfapyrimidines; Troleandomycin; Trospectomycin Sulfate; Tyrothricin; Vancomycin; Vancomycin ... 68 -sodium lauryl sulfate, dioctyl sodium sulfosuccinate and dioctyl sodium sulfonate. Cationic detergents might be used and ...
... butirosin sulfate MeSH D09.408.051.374 --- gentamicins MeSH D09.408.051.374.785 --- sisomicin MeSH D09.408.051.374.785.525 --- ... heparan sulfate proteoglycan MeSH D09.698.373.475 --- hyaluronic acid MeSH D09.698.373.550 --- keratan sulfate MeSH D09.698. ... chondroitin sulfates MeSH D09.698.373.200.380 --- dermatan sulfate MeSH D09.698.373.400 --- heparin MeSH D09.698.373.400.300 ... heparan sulfate proteoglycan MeSH D09.698.735.800 --- platelet factor 4 MeSH D09.698.735.830 --- proteochondroitin sulfates ...
... or butirosin sulfate? or calcimycin? or candicidin? or capreomycin? or carbenicillin? or carfecillin? or cefaclor? or ... or dihydrostreptomycin sulfate? or diketopiperazine? or distamycin? or doxycycline? or echinomycin? or edeine? or enviomycin? ...
Butirosin Sulfate (0) * Gentamicins (0) * Hygromycin B (0) * Kanamycin (4) * Amikacin (1) ...
... dermatan sulfate Y inflammation proteoglycan dermatan sulfate tumors, X proteoglycans inflammation endothelin tumors, G ... butirosin, butixirate, butobendine, butoconazole, butocrolol, butoctamide, butofilolol, butonate, butopamine, butopiprine, ... capreomycin sulfate, cyclosexine, ethambutol, ethionamide, pyrazinamide, rifampin or streptomycin sulphate; antivirals such as ... 0340] DSPS (Avanti, 5.0 mg), lipopeptide from a)(0.3 mg)and polymixin B sulphate (Sigma, 0.5 mg) were weighed into a clean vial ...
Nickel sulfate hexahydrate; sulfuric acid, nickel (2+) salt, hexahydrate; nickel monosulfate hexahydrate; Blue salt; single ... butirosin, bacitracin. ICU. ...
"Neomycin sulfate, EP Susceptibility and Minimum Inhibitory Concentration (MIC) Data" (PDF). TOKU-E. Cammack, R. Attwood, T. K. ... "Unique O-ribosylation in the biosynthesis of butirosin". Bioorganic & Medicinal Chemistry. 15 (13): 4360-8. doi:10.1016/j.bmc. ...
EC 4.2.2.20 chondroitin-sulfate-ABC endolyase. EC 4.2.2.21 chondroitin-sulfate-ABC exolyase. EC 4.2.2.22 pectate trisaccharide- ... EC 4.3.2.6 γ-L-glutamyl-butirosin B γ-glutamyl cyclotransferase. EC 4.3.2.7 glutathione-specific γ-glutamylcyclotransferase EC ... EC 4.2.2.8 heparin-sulfate lyase. EC 4.2.2.9 pectate disaccharide-lyase. EC 4.2.2.10 pectin lyase. EC 4.2.2.11 guluronate- ... EC 4.3.1.10 serine-sulfate ammonia-lyase. EC 4.3.1.11 deleted. EC 4.3.1.12 ornithine cyclodeaminase. EC 4.3.1.13 carbamoyl- ...
Antibiotics such as polymyxin B sulfate-neomycin sulfate, cleocin phosphate ® (available from the Upjohn Company, Kalamazoo, ... Additional suitable antibiotics include aminoglycoside antibiotics such as amikacin, butirosin, dideoxykanamycin B (DKP), ...
Aluminum SucroseSulfate, AmmoniumSulfate, BariumSulfate, ButirosinSulfate, CalciumSulfate, ChondroitinSulfate, CopperSulfate, ... Sodium TetradecylSulfate, XylanSulfate, ZincSulfated Glycoprotein 2Sulfated Glycoprotein-2SulfatesSulfates, ChondroitinSulfates ... DermatanSulfate, DextranSulfate, DHASulfate, DHEASulfate, DihydrostreptomycinSulfate, GentamicinSulfate, HeparanSulfate, ... IndoxylSulfate, KeratanSulfate, MagnesiumSulfate, PolypentoseSulfate, PrasteroneSulfate, Sodium DodecylSulfate, Sodium Lauryl ...
Butirosin Sulfate; Capreomycin Sulfate; Carbadox; Carbenicillin Disodium; Carbenicillin Indanyl Sodium; Carbenicillin Phenyl ... vinblastine sulfate; vincristine sulfate; vindesine; vindesine sulfate; vinepidine sulfate; vinglycinate sulfate; vinleurosine ... sulfate; vinorelbine tartrate; vinrosidine sulfate; vinzolidine sulfate; vorozole; zeniplatin; zinostatin; zorubicin ... Neomycin Sulfate; Neomycin Undecylenate; Netilmicin Sulfate; Neutramycin; Nifuradene; Nifuraldezone; Nifuratel; Nifuratrone; ...
0188] (i) carboxylic acids, sulfates and phosphates (e.g. for reacting with a spacer precursor moiety comprising a primary ... Butirosin, Clindamycin, Clomocycline, Chloramphenicol, Cloxacillin, Colistin, Demeclocycline, Deoxydihydrostreptomycin, ... The filtrate was washed with 0.01M HCl solution (3×50 ml), dried over sodium sulfate, filtered and concentrated under reduced ... 0222] Amino-, Hydroxyl-, and Carboxyl-containing (including sulfate, phosphate and phosphonate-containing): p-Aminosalicylic ...
Butirosin Sulfate , Kanamycin , Tobramycin , Paromomycin , Aminoglycosides - chemical synthesis ... Butirosin Sulfate - biosynthesis , Kanamycin - biosynthesis , Anti-Bacterial Agents - biosynthesis , Cyclohexanols - ...
Butirosin Sulfate - pharmacology , Escherichia coli - drug effects , Humans , Kanamycin - pharmacology , Stenotrophomonas ...
Butirosin B has been identified as1-N-[(S)-4-amino-2-hydroxy-n-butyryl]-ribostamycin; see the "Tetrahedron Letters" Vol. 28, ... sulfate, phosphate, acetate, maleate, fumarate, succinate,tartrate, oxalate, citrate, methanesulfonate, ethanesulfonate and the ... On the other hand, it is known that butirosin B which is an aminoglycosidic antibiotic produced by a Bacillus species is active ... 4-amino-2-hydroxybutyryl substituent on the 1-amino group of the butirosin B molecule has an important role in enabling the ...
Kanamycin Sulfate \ 101-25389-94-0 for more molecular products just contact us ... aphA4 aph butA] Aminoglycoside 3-phosphotransferase (EC 2.7.1.95) (APH(3)IV) (Butirosin resistance protein A) (Kanamycin ... We have also other products like : Kanamycin Sulfate Related products : Kanamycin Sulfate ... WP579: Sulfate assimilation pathway II. Related Genes :. [kamB] 16S rRNA (adenine(1408)-N(1))-methyltransferase (EC 2.1.1.180 ...
... butirosin, macrolactin, bacillaene, fengycin, lanthipeptides and LCI, were predicted in the genome of Bacillus sp. GFP-2, which ... 2016). Butirosin, an aminoglycoside antimicrobial peptide, is known as the important class of agents, especially against ... GFP-2 were extracted by ammonium sulfate precipitation method and the glucan hydrolysis assay was performed. As shown in Fig. 4 ... GFP-2 were extracted by ammonium sulfate precipitation method from the culture supernatant. Then 200 μl of Na3PO4 buffer (a), ...
The spheroids contain 93 wt % of collagen, 5 wt % of hydrolyzed collagen (protein powder) and 2 wt % of gentamycin sulphate. ... butirosin, didesoxykanamycin B (DKB), fortimycin, gentamycin, kanamycin, lividomycin, neomycin, Netilmycin, ribostamycin, ... The platelets contain 93 wt % of collagen, 5 wt % of the copolymer and about 1.9 wt % of gentamycin sulphate. ... The shaped particles contain 92 wt % of collagen, about 5% of copolymer and 2.9% of gentamycin sulphate. ...
067-01731 Fradiomycin Sulfate(Neomycin Sulfate) 5g. 065-01732 Fradiomycin Sulfate(Neomycin Sulfate) 25g. 546-00171 Genistein(4 ... 024-07521 Butirosin A 10mg. 035-14441 Calcium lonophore A23187 10mg. 031-14622 Carbadox 25g. 039-11041 Carbenicillin Sodium 1g ... 019-04851 Atropine Sulfate Monohydrate 1g. 015-04853 Atropine Sulfate Monohydrate 1g. 013-04854 Atropine Sulfate Monohydrate 1g ... 073-02971 Gentamicin Sulfate 250mg. 079-02973 Gentamicin Sulfate 1g. 077-02974 Gentamicin Sulfate 5g. 073-02231 Griseofulvin 5g ...
Neomycin Paromomycin Ribostamycin and Butirosin. The extensive work of Rinehart and his group has shown that the individual ... described a synthetic medium for tyrosine production consisting of glucose betaine glycine potassium nitrate magnesium sulfate ... In the biosynthesis of ribostamycin and butirosin in S. ribosidificus and B. cirulans ribose seems to be added last. More ... And the butiorosin pathway proposed by Takeda a biosynthetic pathoway for ribostamycin neomycin paromomycin and butirosin may ...
In monomer O two half-occupancy sulfate ions were found at the active site, which are analogous to the substrate and the " ... Crystal structures of the PLP- and PMP-bound forms of BtrR, a dual functional aminotransferase involved in butirosin ... One half-occupancy sulfate ion is also located in the substrate-binding site of monomer P. ... The aminotransferase (BtrR), which is involved in the biosynthesis of butirosin, a 2-deoxystreptamine (2-DOS)-containing ...
Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate_Homo sapiens_hsa00532. 0.57539880. 77. Chemical ... Butirosin and neomycin biosynthesis_Homo sapiens_hsa00524. 0.96417186. 51. Legionellosis_Homo sapiens_hsa05134. 0.96367839. ...
Glycosaminoglycan biosynthesis - chondroitin sulfate / dermatan sulfate_Homo sapiens_hsa00532. 1.66779219. 16. Fatty acid ... Butirosin and neomycin biosynthesis_Homo sapiens_hsa00524. 0.91161642. 51. Primary bile acid biosynthesis_Homo sapiens_hsa00120 ... heparan sulfate proteoglycan biosynthetic process (GO:0015012). 2.36464981. 76. glutamine family amino acid biosynthetic ... Glycosaminoglycan biosynthesis - heparan sulfate / heparin_Homo sapiens_hsa00534. 1.50397885. 19. Amino sugar and nucleotide ...
sulfate in order to form a complex. This complex can be dissolved in an organic. solvent which can then be dip coated or spray ... butirosin, capreomycin, carbenicillin, carbomycin, carumonam, cefadroxil,. cefamandole, cefatrizine, cefbuperazone, cefclidin, ... wherein X and Y are anionic leaving groups such as sulfate, phosphate,. carboxylate, and halogen, R1 and R2 are alkyl, amine, ... halogen, hydroxy, carboxylate, ester, sulfate or phosphate. See, e.g., U.S. Patent. Nos. 4,588,831 and 4,250,189.. Suitable ...