Burkholderia pseudomallei: A species of gram-negative, aerobic bacteria that causes MELIOIDOSIS. It has been isolated from soil and water in tropical regions, particularly Southeast Asia.Melioidosis: A disease of humans and animals that resembles GLANDERS. It is caused by BURKHOLDERIA PSEUDOMALLEI and may range from a dormant infection to a condition that causes multiple abscesses, pneumonia, and bacteremia.Burkholderia: A genus of gram-negative, aerobic, rod-shaped bacteria. Organisms in this genus had originally been classified as members of the PSEUDOMONAS genus but overwhelming biochemical and chemical findings indicated the need to separate them from other Pseudomonas species, and hence, this new genus was created.Burkholderia mallei: A species of gram-negative bacteria parasitic on HORSES and DONKEYS causing GLANDERS, which can be transmitted to humans.Burkholderia Infections: Infections with bacteria of the genus BURKHOLDERIA.Burkholderia cepacia: A species of BURKHOLDERIA considered to be an opportunistic human pathogen. It has been associated with various types of infections of nosocomial origin.Glanders: A contagious disease of horses that can be transmitted to humans. It is caused by BURKHOLDERIA MALLEI and characterized by ulceration of the respiratory mucosa and an eruption of nodules on the skin.Burkholderia cepacia complex: A group of phenotypically similar but genotypically distinct species (genomovars) in the genus BURKHOLDERIA. They are found in water, soil, and the rhizosphere of crop plants. They can act as opportunistic human pathogens and as plant growth promoting and biocontrol agents.Burkholderia cenocepacia: A species of gram-negative bacteria that causes disease in plants. It is found commonly in the environment and is an opportunistic pathogen in humans.Thailand: Formerly known as Siam, this is a Southeast Asian nation at the center of the Indochina peninsula. Bangkok is the capital city.Ceftazidime: Semisynthetic, broad-spectrum antibacterial derived from CEPHALORIDINE and used especially for Pseudomonas and other gram-negative infections in debilitated patients.Biological Warfare Agents: Living organisms or their toxic products that are used to cause disease or death of humans during WARFARE.Soil Microbiology: The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Virulence: The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.Environmental Microbiology: The study of microorganisms living in a variety of environments (air, soil, water, etc.) and their pathogenic relationship to other organisms including man.Northern Territory: Territory in north central Australia, between the states of Queensland and Western Australia. Its capital is Darwin.Burkholderia gladioli: A species of gram-negative, aerobic bacteria that acts as both a human and plant pathogen.Bacterial Proteins: Proteins found in any species of bacterium.Microbial Viability: Ability of a microbe to survive under given conditions. This can also be related to a colony's ability to replicate.ArabinoseAnti-Bacterial Agents: Substances that reduce the growth or reproduction of BACTERIA.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Genome, Bacterial: The genetic complement of a BACTERIA as represented in its DNA.Virulence Factors: Those components of an organism that determine its capacity to cause disease but are not required for its viability per se. Two classes have been characterized: TOXINS, BIOLOGICAL and surface adhesion molecules that effect the ability of the microorganism to invade and colonize a host. (From Davis et al., Microbiology, 4th ed. p486)Bacterial Typing Techniques: Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Mice, Inbred BALB CQuorum Sensing: A phenomenon where microorganisms communicate and coordinate their behavior by the accumulation of signaling molecules. A reaction occurs when a substance accumulates to a sufficient concentration. This is most commonly seen in bacteria.Genes, Bacterial: The functional hereditary units of BACTERIA.Trimethoprim-Sulfamethoxazole Combination: This drug combination has proved to be an effective therapeutic agent with broad-spectrum antibacterial activity against both gram-positive and gram-negative organisms. It is effective in the treatment of many infections, including PNEUMOCYSTIS PNEUMONIA in AIDS.Gene Expression Regulation, Bacterial: Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.Australia: The smallest continent and an independent country, comprising six states and two territories. Its capital is Canberra.Bacteriological Techniques: Techniques used in studying bacteria.Acyl-Butyrolactones: Cyclic esters of acylated BUTYRIC ACID containing four carbons in the ring.Suppuration: A pathologic process consisting in the formation of pus.Asia, Southeastern: The geographical area of Asia comprising BORNEO; BRUNEI; CAMBODIA; INDONESIA; LAOS; MALAYSIA; the MEKONG VALLEY; MYANMAR (formerly Burma), the PHILIPPINES; SINGAPORE; THAILAND; and VIETNAM.Antibodies, Bacterial: Immunoglobulins produced in a response to BACTERIAL ANTIGENS.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Arginine: An essential amino acid that is physiologically active in the L-form.Adhesins, Bacterial: Cell-surface components or appendages of bacteria that facilitate adhesion (BACTERIAL ADHESION) to other cells or to inanimate surfaces. Most fimbriae (FIMBRIAE, BACTERIAL) of gram-negative bacteria function as adhesins, but in many cases it is a minor subunit protein at the tip of the fimbriae that is the actual adhesin. In gram-positive bacteria, a protein or polysaccharide surface layer serves as the specific adhesin. What is sometimes called polymeric adhesin (BIOFILMS) is distinct from protein adhesin.Bacterial Adhesion: Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity.Adhesins, Escherichia coli: Thin, filamentous protein structures, including proteinaceous capsular antigens (fimbrial antigens), that mediate adhesion of E. coli to surfaces and play a role in pathogenesis. They have a high affinity for various epithelial cells.Brucella suis: A species of gram-negative bacteria, primarily infecting SWINE, but it can also infect humans, DOGS, and HARES.Pseudomonas: A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants.PubMed: A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.Methacycline: A broad-spectrum semisynthetic antibiotic related to TETRACYCLINE but excreted more slowly and maintaining effective blood levels for a more extended period.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.Inorganic Pyrophosphatase: An enzyme which catalyzes the hydrolysis of diphosphate (DIPHOSPHATES) into inorganic phosphate. The hydrolysis of pyrophosphate is coupled to the transport of HYDROGEN IONS across a membrane.Pyrophosphatases: A group of enzymes within the class EC 3.6.1.- that catalyze the hydrolysis of diphosphate bonds, chiefly in nucleoside di- and triphosphates. They may liberate either a mono- or diphosphate. EC 3.6.1.-.Genomic Islands: Distinct units in some bacterial, bacteriophage or plasmid GENOMES that are types of MOBILE GENETIC ELEMENTS. Encoded in them are a variety of fitness conferring genes, such as VIRULENCE FACTORS (in "pathogenicity islands or islets"), ANTIBIOTIC RESISTANCE genes, or genes required for SYMBIOSIS (in "symbiosis islands or islets"). They range in size from 10 - 500 kilobases, and their GC CONTENT and CODON usage differ from the rest of the genome. They typically contain an INTEGRASE gene, although in some cases this gene has been deleted resulting in "anchored genomic islands".

Efflux-mediated aminoglycoside and macrolide resistance in Burkholderia pseudomallei. (1/670)

Burkholderia pseudomallei, the causative agent of melioidosis, is intrinsically resistant to a wide range of antimicrobial agents including beta-lactams, aminoglycosides, macrolides, and polymyxins. We used Tn5-OT182 to mutagenize B. pseudomallei to identify the genes involved in aminoglycoside resistance. We report here on the identification of AmrAB-OprA, a multidrug efflux system in B. pseudomallei which is specific for both aminoglycoside and macrolide antibiotics. We isolated two transposon mutants, RM101 and RM102, which had 8- to 128-fold increases in their susceptibilities to the aminoglycosides streptomycin, gentamicin, neomycin, tobramycin, kanamycin, and spectinomycin. In addition, both mutants, in contrast to the parent, were susceptible to the macrolides erythromycin and clarithromycin but not to the lincosamide clindamycin. Sequencing of the DNA flanking the transposon insertions revealed a putative operon consisting of a resistance, nodulation, division-type transporter, a membrane fusion protein, an outer membrane protein, and a divergently transcribed regulatorprotein. Consistent with the presence of an efflux system, both mutants accumulated [3H] dihydro streptomycin, whereas the parent strain did not. We constructed an amr deletion strain, B. pseudomallei DD503, which was hypersusceptible to aminoglycosides and macrolides and which was used successfully in allelic exchange experiments. These results suggest that an efflux system is a major contributor to the inherent high-level aminoglycoside and macrolide resistance found in B. pseudomallei.  (+info)

Phylogenetic analysis of Ara+ and Ara- Burkholderia pseudomallei isolates and development of a multiplex PCR procedure for rapid discrimination between the two biotypes. (2/670)

A Burkholderia pseudomallei-like organism has recently been identified among some soil isolates of B. pseudomallei in an area with endemic melioidosis. This organism is almost identical to B. pseudomallei in terms of morphological and biochemical profiles, except that it differs in ability to assimilate L-arabinose. These Ara+ isolates are also less virulent than the Ara- isolates in animal models. In addition, clinical isolates of B. pseudomallei available to date are almost exclusively Ara-. These features suggested that these two organisms may belong to distinctive species. In this study, the 16S rRNA-encoding genes from five clinical (four Ara- and one Ara+) and nine soil isolates (five Ara- and four Ara+) of B. pseudomallei were sequenced. The nucleotide sequences and phylogenetic analysis indicated that the 16S rRNA-encoding gene of the Ara+ biotype was similar to but distinctively different from that of the Ara- soil isolates, which were identical to the classical clinical isolates of B. pseudomallei. The nucleotide sequence differences in the 16S rRNA-encoding gene appeared to be specific for the Ara+ or Ara- biotypes. The differences were, however, not sufficient for classification into a new species within the genus Burkholderia. A simple and rapid multiplex PCR procedure was developed to discriminate between Ara- and Ara+ B. pseudomallei isolates. This new method could also be incorporated into our previously reported nested PCR system for detecting B. pseudomallei in clinical specimens.  (+info)

Characterization of a murine model of melioidosis: comparison of different strains of mice. (3/670)

Melioidosis is an infectious disease caused by the saprophytic gram-negative rod Burkholderia pseudomallei. The aim of this study was to establish and characterize a murine model of melioidosis to provide a basis for further investigations on the pathogenesis of the disease. After intravenous infection with B. pseudomallei, C57BL/6 mice were found to be significantly more resistant than BALB/c mice. There was a marked organotropism of B. pseudomallei for the spleen and liver in both strains of mice, with the highest bacterial load in the spleen. Electron microscopic investigations of the spleen clearly demonstrated intracellular replication within membrane-bound phagosomes. Electron micrographs of the liver provided evidence that B. pseudomallei-containing phagosomes in hepatocytes fuse with lysosomes, leading to degradation of bacteria. In both strains of mice, the course of infection was highly dependent on the infective dose and the bacterial strain used, ranging from death within a few days to death after several weeks. In comparison with BALB/c mice, the bacterial counts in C57BL/6 mice were decreased 12 h after infection, which is suggestive of an innate immune mechanism against B. pseudomallei in this early phase of infection contributing to the lower susceptibility of C57BL/6 mice. BALB/c mice developed a more pronounced lymphopenia, granulocytosis, and splenomegaly at a lower infective dose compared to C57BL/6 mice. Analysis of the antibody response against B. pseudomallei 11 days after infection revealed a significantly higher immunoglobulin G2A (IgG2a)/IgG1 ratio in C57BL/6 mice than in BALB/c mice, indicating that a T helper type 1 immune response is associated with resistance to infection with B. pseudomallei.  (+info)

Obligatory role of gamma interferon for host survival in a murine model of infection with Burkholderia pseudomallei. (4/670)

Burkholderia pseudomallei, the causative agent of melioidosis, is a gram-negative bacterium capable of causing either acute lethal sepsis or chronic but eventually fatal disease in infected individuals. However, despite the clinical importance of this infection in areas where it is endemic, there is essentially no information on the mechanisms of protective immunity to the bacterium. We describe here a murine model of either acute or chronic infection with B. pseudomallei in Taylor Outbred (TO) mice which mimics many features of the human pathology. Intraperitoneal infection of TO mice at doses of >10(6) CFU resulted in acute septic shock and death within 2 days. In contrast, at lower doses mice were able to clear the inoculum from the liver and spleen over a 3- to 4-week period, but persistence of the organism at other sites resulted in a chronic infection of between 2 and 16 months duration which was eventually lethal in all of the animals tested. Resistance to acute infection with B. pseudomallei was absolutely dependent upon the production of gamma interferon (IFN-gamma) in vivo. Administration of neutralizing monoclonal antibody against IFN-gamma lowered the 50% lethal dose from >5 x 10(5) to ca. 2 CFU and was associated with 8,500- and 4,400-fold increases in the bacterial burdens in the liver and spleen, respectively, together with extensive destruction of lymphoid architecture in the latter organ within 48 h. Neutralization of either tumor necrosis factor alpha or interleukin-12 but not granulocyte-macrophage colony-stimulating factor, also increased susceptibility to infection in vivo. Together, these results provide the first evidence of a host protective mechanism against B. pseudomallei. The rapid production of IFN-gamma within the first day of infection determines whether the infection proceeds to an acute lethal outcome or becomes chronic.  (+info)

Evidence for the presence in Burkholderia pseudomallei of a type III secretion system-associated gene cluster. (5/670)

Burkholderia pseudomallei, the causative agent of melioidosis, contains a cluster of putative genes homologous to those encoding HpaP, HrcQ, HrcR, HrcS and HrpV in the plant pathogen Ralstonia solanacearum. In R. solanacearum, these genes form part of a type III secretion-associated pathogenicity island. The order of the genes in B. pseudomallei is directly equivalent to that found in R. solanacearum. The B. pseudomallei proteins share 49.5% (HpaP), 52.6% (HrcQ), 80.0% (HrcR), 72.1% (HrcS) and 46.7% (HrpV) similarity, respectively, with their equivalent R. solanacearum proteins. The presence of type III secretion-associated genes in B. pseudomallei pathogens suggests a possible role for type III secretion systems in the pathogenicity of this organism.  (+info)

Molecular characterization of genetic loci required for secretion of exoproducts in Burkholderia pseudomallei. (6/670)

Previous studies have demonstrated that Burkholderia pseudomallei secretes protease, lipase, and phospholipase C (PLC) into the extracellular milieu, but their mechanisms of secretion and roles in pathogenesis have not been elucidated. In this study, we isolated and characterized 29 transposon mutants unable to secrete protease, lipase, and PLC.  (+info)

Melioidosis with adrenal gland abscess. (7/670)

We report a case of melioidosis with left adrenal gland abscess in a 51-year-old man from Taiwan who traveled to Rangoon, Burma for a four-day tour on July 15, 1997. The patient developed fever and left upper abdominal pain upon returning to Taiwan on July 19, 1997. Ten days after returning to Taiwan, he was admitted to Chang Gung Memorial Hospital in Keelung, Taiwan and blood culture on admission was positive for Burkholderia pseudomallei. Computerized tomography of the abdomen revealed left adrenal gland swelling and suppuration. Treatment with parenteral ceftazidime and cotrimoxazole for three weeks followed by two months of oral cotrimoxazole cured the infection. The patient remained asymptomatic at 12 months follow-up.  (+info)

Epidemiology of Burkholderia pseudomallei in Thailand. (8/670)

The distribution of Burkholderia pseudomallei in soil collected from four regions of Thailand and the frequency of B. pseudomallei infections in patients attending government hospitals throughout Thailand in 1997 were surveyed. A total of 3,585 soil samples collected from 896 sites in four regions of Thailand were cultured for B. pseudomallei using selective enrichment broth and modified Ashdown's agar. The organism was recovered in 4.4%, 6.1%, 20.4%, and 5.9% of the soil samples collected from the northern, central, northeastern, and southern regions, respectively, of Thailand (P < 0.0001). Burkholderia pseudomallei was cultured from 50.1% of the sites in the northeastern region compared with 13.8%, 24.5%, and 18.4% in the northern, central, and southern regions, respectively (P < 0.0001). The infection rate in patients attending government hospitals in the northeastern region (137.9 per 100,000 inpatients) was significantly higher than those in the northern (18 per 100,000 inpatients), central (13.4 per 100,000 inpatients), and southern (14.4 per 100,000 inpatients) regions, respectively (P < 0.0001). It is suggested that melioidosis, which is endemic in Thailand, is associated with the presence of B. pseudomallei in soil.  (+info)

Neurologic melioidosis is a serious, potentially fatal form of Burkholderia pseudomallei infection. Recently, we reported that a subset of clinical isolates of B. pseudomallei from Australia have heightened virulence and potential for dissemination to the central nervous system. In this study, we demonstrate that this subset has a B. mallei-like sequence variation of the actin-based motility gene, bimA. Compared with B. pseudomallei isolates having typical bimA alleles, isolates that contain the B. mallei-like variation demonstrate increased persistence in phagocytic cells and increased virulence with rapid systemic dissemination and replication within multiple tissues, including the brain and spinal cord, in an experimental model. These findings highlight the implications of bimA variation on disease progression of B. pseudomallei infection and have considerable clinical and public health implications with respect to the degree of neurotropic threat posed to human health.
Neurologic melioidosis is a serious, potentially fatal form of Burkholderia pseudomallei infection. Recently, we reported that a subset of clinical isolates of B. pseudomallei from Australia have heightened virulence and potential for dissemination to the central nervous system. In this study, we demonstrate that this subset has a B. mallei-like sequence variation of the actin-based motility gene, bimA. Compared with B. pseudomallei isolates having typical bimA alleles, isolates that contain the B. mallei-like variation demonstrate increased persistence in phagocytic cells and increased virulence with rapid systemic dissemination and replication within multiple tissues, including the brain and spinal cord, in an experimental model. These findings highlight the implications of bimA variation on disease progression of B. pseudomallei infection and have considerable clinical and public health implications with respect to the degree of neurotropic threat posed to human health.
Citation. Tumapa, S., Holden, M. T., Vesaratchavest, M., Wuthiekanun, V., Limmathurotsakul, D., Chierakul, W., Feil, E. J., Currie, B. J., Day, N. P., Nierman, W. C., Peacock, S. J.. Burkholderia pseudomallei Genome Plasticity Associated With Genomic Island Variation. BMC Genomics. 2008 Apr 25; 9(1): 190.. PubMed Citation. Abstract. ABSTRACT: BACKGROUND: Burkholderia pseudomallei is a soil-dwelling saprophyte and the cause of melioidosis. Horizontal gene transfer contributes to the genetic diversity of this pathogen and may be an important determinant of virulence potential. The genome contains genomic island (GI) regions that encode a broad array of functions. Although there is some evidence for the variable distribution of genomic islands in B. pseudomallei isolates, little is known about the extent of variation between related strains or their association with disease or environmental survival. RESULTS: Five islands from B. pseudomallei strain K96243 were chosen as representatives of ...
Cystic fibrosis (CF) is a genetic disorder characterized by progressive lung function decline. CF patients are at an increased risk of respiratory infections, including those by the environmental bacterium Burkholderia pseudomallei, the causative agent of melioidosis. Here, we compared the genomes of B. pseudomallei isolates collected between similar to 4 and 55 months apart from seven chronically infected CF patients. Overall, the B. pseudomallei strains showed evolutionary patterns similar to those of other chronic infections, including emergence of antibiotic resistance, genome reduction, and deleterious mutations in genes involved in virulence, metabolism, environmental survival, and cell wall components. We documented the first reported B. pseudomallei hypermutators, which were likely caused by defective MutS. Further, our study identified both known and novel molecular mechanisms conferring resistance to three of the five clinically important antibiotics for melioidosis treatment. Our ...
Melioidosis caused by the environmental gram-negative bacillus Burkholderia pseudomallei, is classically characterized by pneumonia and multiple abscesses, wit...
TY - JOUR. T1 - Epidemiological tracking and population assignment of the non-clonal bacterium, burkholderia pseudomallei. AU - Dale, Julia. AU - Price, Erin P.. AU - Hornstra, Heidie. AU - Busch, Joseph D.. AU - Mayo, Mark. AU - Godoy, Daniel. AU - Wuthiekanun, Vanaporn. AU - Baker, Anthony. AU - Foster, Jeffrey T. AU - Wagner, David M. AU - Tuanyok, Apichai. AU - Warner, Jeffrey. AU - Spratt, Brian G.. AU - Peacock, Sharon J.. AU - Currie, Bart J.. AU - Keim, Paul S. AU - Pearson, Talima R. PY - 2011/12. Y1 - 2011/12. N2 - Rapid assignment of bacterial pathogens into predefined populations is an important first step for epidemiological tracking. For clonal species, a single allele can theoretically define a population. For non-clonal species such as Burkholderia pseudomallei, however, shared allelic states between distantly related isolates make it more difficult to identify population defining characteristics. Two distinct B. pseudomallei populations have been previously identified using ...
Ceftazidime is the antibiotic of choice for treatment of Burkholderia pseudomallei infections (melioidosis). The chromosomally encoded PenA β-lactamase possesses weak cephalosporinase activity. The wild-type penA gene confers clinically significant ceftazidime resistance only when overexpressed due to a promoter mutation, transcriptional anti-termination or by gene duplication and amplification (GDA). Here we characterize a reversible 33-kb GDA event involving wild-type penA in a ceftazidime resistant clinical isolate from Thailand. We show that duplication arises from exchanges between short (|10 base pairs, bp) chromosomal sequences, which in this example consist of 4 bp repeats flanked by 3 bp inverted repeats. GDA involving β-lactamase may be a common ceftazidime resistance mechanism in B. pseudomallei.
Burkholderia pseudomallei, the etiological agent of melioidosis, is a saprophytic bacterium existing endemically in the water and soil of SE Asia and Northern Australia. This organism has shown the ability to remain dormant in its host for decades. B. thailandensis is a closely related non-pathogenic near neighbor that is also found in these soils. It has been suggested that free-living amoeba could be natural reservoirs for these organisms. The interactions of Burkholderia species and Acanthamoeba castellanii, a species of free-living amoeba, were studied to better understand the natural ecology of these organisms and to determine the effects amoeba interactions might have on pathogenesis. In this study, the adherence and persistence of several B. pseudomallei clinical isolates were compared to that of B. thailandensis within both amoeba and a human monocyte cell line. Results showed that B. pseudomallei isolates can enter amoeba and survive therein at varying levels of efficiency. Some isolates were
TY - JOUR. T1 - Burkholderia pseudomallei Antibodies in Individuals Living in Endemic Regions in Northeastern Brazil. AU - Rolim, D.B.. AU - Vilar, D.C.F.L.. AU - De Goes Cavalcanti, L.P.. AU - Freitas, C.L.B.N.. AU - Inglis, Tim. AU - Nobre Rodrigues, J.L.N.. AU - Nagao-Dias, A.T.. PY - 2011. Y1 - 2011. N2 - A seroepidemiological investigation was conducted among the population of two municipalities in Northeastern Brazil. Immunoglobulin M (IgM) and IgG antibodies to Burkholderia pseudomallei were positive in 51.27% (161 in 317 samples) and 58.49% (186), respectively. IgM titers were higher in children than in adults. On the contrary, IgG increased progressively with age. We observed a significant association between agricultural occupation and raised IgM titers (P ,0.005) and IgG titers (P ,0.001), and between construction workers and raised IgG titers (P = 0.005). Antibody IgG avidities did not correlate with age. The highest titers of antibodies (1/800) showed the highest antibody avidity ...
Easton A, Haque https://www.ncbi.nlm.nih.gov/pubmed/?term=19829050%5Bpmid%5DA, Chu K, Lukaszewski R, Bancroft GJ. 2007. A critical role for neutrophils in resistance to experimental infection with Burkholderia pseudomallei. J Infect Dis 195: 99-107. A Critical Role for Neutrophils in Resistance to Experimental Infection with Burkholderia pseudomallei. ...
Background Burkholderia pseudomallei is the causative agent of melioidosis, a disease of significant morbidity and mortality in both human and animals in endemic areas. There is no vaccine towards the bacterium available in the market, and the efficacy of many of the bacteriums surface and secreted proteins are currently being evaluated as vaccine candidates. Methodology/Principal Findings With the availability of the B. pseudomallei whole genome sequence, we undertook to identify genes encoding the known immunogenic outer membrane protein A (OmpA). Twelve OmpA domains were identified and ORFs containing these domains were fully annotated. Of the 12 ORFs, two of these OmpAs, Omp3 and Omp7, were successfully cloned, expressed as soluble protein and purified. Both proteins were recognised by antibodies in melioidosis patients sera by Western blot analysis. Purified soluble fractions of Omp3 and Omp7 were assessed for their ability to protect BALB/c mice against B. pseudomallei infection. Mice were
Burkholderia pseudomallei (also known as Pseudomonas pseudomallei) is a Gram-negative, bipolar, aerobic, motile rod-shaped bacterium. It is a soil-dwelling bacterium endemic in tropical and subtropical regions worldwide, particularly in Thailand and northern Australia. It infects humans and animals and causes the disease melioidosis. It is also capable of infecting plants. B. pseudomallei measures 2-5 μm in length and 0.4-0.8 μm in diameter and is capable of self-propulsion using flagella. The bacteria can grow in a number of artificial nutrient environments, especially betaine- and arginine-containing ones. In vitro, optimal proliferation temperature is reported around 40 °C in neutral or slightly acidic environments (pH 6.8-7.0). The majority of strains are capable of fermentation of sugars without gas formation (most importantly, glucose and galactose; older cultures are reported to also metabolize maltose and starch). Bacteria produce both exo- and endotoxins. The role of the toxins ...
Kynurenine formamidase (KynB) forms part of the kynurenine pathway which metabolises tryptophan to anthranilate. This metabolite can be used for downstream production of 2-alkyl-4-quinolone (AQ) signalling molecules that control virulence in Pseudomonas aeruginosa. Here we investigate the role of kynB in the production of AQs and virulence-associated phenotypes of Burkholderia pseudomallei K96243, the causative agent of melioidosis. Deletion of kynB resulted in reduced AQ production, increased biofilm formation, decreased swarming and increased tolerance to ciprofloxacin. Addition of exogenous anthranilic acid restored the biofilm phenotype, but not the persister phenotype. This study suggests the kynurenine pathway is a critical source of anthranilate and signalling molecules that may regulate B. pseudomallei virulence ...
Burkholderia pseudomallei is the causative agent of melioidosis, a fatal human tropical disease. The non-specific DNA-binding protein DpsA plays a key role in protecting B. pseudomallei from oxidative stress mediated, for example, by organic hydroperoxides. The regulation of dpsA expression is poorly understood but one possibility is that it is regulated in a cell population density-dependent manner via N-acylhomoserine lactone (AHL)-dependent quorum sensing (QS) since a lux-box motif has been located within the dpsA promoter region. Using liquid chromatography and tandem mass spectrometry, it was first established that B. pseudomallei strain PP844 synthesizes AHLs. These were identified as N-octanoylhomoserine lactone (C8-HSL), N-(3-oxooctanoyl)homoserine lactone (3-oxo-C8-HSL), N-(3-hydroxyoctanoyl)-homoserine lactone (3-hydroxy-C8-HSL), N-decanoylhomoserine lactone (C10-HSL), N-(3-hydroxydecanoyl) homoserine lactone (3-hydroxy-C10-HSL) and N-(3-hydroxydodecanoyl)homoserine lactone (3-hydroxy-C12-HSL)
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Burkholderia pseudomallei is a Gram-negative bacterium that causes the serious human disease, melioidosis. There is no vaccine against melioidosis and it can be fatal if not treated with a specific antibiotic regimen, which typically includes the third-generation cephalosporin, ceftazidime (CAZ). There have been several resistance mechanisms described for B. pseudomallei, of which the best described are amino acid changes that alter substrate specificity in the highly conserved class A β-lactamase, PenA. In the current study, we sequenced penA from isolates sequentially derived from two melioidosis patients with wild-type (1.5 µg/mL) and, subsequently, resistant (16 or ≥256 µg/mL) CAZ phenotypes. We identified two single-nucleotide polymorphisms (SNPs) that directly increased CAZ hydrolysis. One SNP caused an amino acid substitution (C69Y) near the active site of PenA, whereas a second novel SNP was found within the penA promoter region. In both instances, the CAZ resistance phenotype ...
Burkholderia pseudomallei is the causative agent of the disease melioidosis, which is endemic in the tropics and a concern elsewhere as a biological warfare agent. Currently no human vaccine exists either in clinical trials or in a licensed form. Recently passively transferred monoclonal antibodies directed toward the expolysaccharide of B. pseudomallei have been shown to impart survival when administered prior to lethal challenge and active immunization using purified exopolysaccharide extends the mean time to death. Short peptides, termed mimotopes, mimicking native carbohydrate have been developed and used to induce protective responses against extracellular bacteria. Here the ability of mimotopes to generate a protective response against a pathogen that can invade host cells was investigated. Mimotopes immunoreactive to the passive protective monoclonals were developed and used to generate an antibody response against B. pseudomallei. Preliminary evaluation of the mimotopes in a murine ...
Burkholderia pseudomallei is a human and animal pathogen in tropical regions, especially Southeast Asia and northern Australia. Currently little is known about the genetics and molecular biology of this organism. In this report, we describe the mutagenesis of B. pseudomallei with the transposon Tn5-OT182. B. pseudomallei 1026b transposon mutants were obtained at a frequency of 4.6 x 10(-4) per initial donor cell, and the transposon inserted randomly into the chromosome. We used Tn5-OT182 to identify the flagellin structural gene, fliC. We screened 3,500 transposon mutants and identified 28 motility mutants. Tn5-OT182 integrated into 19 unique genetic loci encoding proteins with homology to Escherichia coli and Salmonella typhimurium flagellar and chemotaxis proteins. Two mutants, MM35 and MM36, contained Tn5-OT182 integrations in fliC. We cloned and sequenced fliC and used it to complement MM35 and MM36 in trans. The fliC transcriptional start site and a sigmaF-like promoter were identified by ...
Abstract Burkholderia pseudomallei is the causative agent of melioidosis, a severe infection endemic to many tropical regions. Lipopolysaccharide (LPS) is recognized as an important virulence factor used by B. pseudomallei. Isolates of B. pseudomallei have been shown to express one of four different types of LPS (typical LPS, atypical LPS types B and B2, and rough LPS) and in vitro studies have demonstrated that LPS types may impact disease severity. The association between LPS types and clinical manifestations, however, is still unknown, in part because an effective method for LPS type identification is not available. Thus, we developed antigen capture immunoassays capable of distinguishing between the LPS types. Mice were injected with B or B2 LPS for atypical LPS-specific monoclonal antibody (mAb) isolation; only two mAbs (3A2 and 5B4) were isolated from mice immunized with B2 LPS. Immunoblot analysis and surface plasmon resonance demonstrated that 3A2 and 5B4 are reactive with both B2 and B LPS
Burkholderia pseudomallei is resistant to a diverse group of antimicrobials including third generation cephalosporins whilst quinolones and aminoglycosides have no reliable effect. As therapeutic options are limited, development of more effective forms of immunotherapy is vital to avoid a fatal outcome. In an earlier study, we reported on the B. pseudomallei serine MprA protease which is relatively stable over a wide pH and temperature range and digests physiological proteins. The present study was carried out to evaluate the immunogenicity and protective efficacy of the MprA as a potential vaccine candidate. In BALB/c mice immunized with recombinant MprA protease (smBpF4), a significantly high IgG titer was detectable. Isotyping studies revealed that the smBpF4-specific antibodies produced were predominantly IgG1, proposing that immunization with smBpF4 triggered a Th2 immune response. Mice were immunized with smBpF4 and subsequently challenged with B. pseudomallei via the intraperitoneal route. Whilst
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Background Pathogenic bacteria adhere to the host cell surface using a family of outer membrane proteins called Trimeric Autotransporter Adhesins (TAAs). Although TAAs are highly divergent in sequence and domain structure, they are all conceptually comprised of a C-terminal membrane anchoring domain and an N-terminal passenger domain. Passenger domains consist of a secretion sequence, a head region that facilitates binding to the host cell surface, and a stalk region. Methodology/Principal Findings Pathogenic species of Burkholderia contain an overabundance of TAAs, some of which have been shown to elicit an immune response in the host. To understand the structural basis for host cell adhesion, we solved a 1.35 Å resolution crystal structure of a BpaA TAA head domain from Burkholderia pseudomallei, the pathogen that causes melioidosis. The structure reveals a novel fold of an intricately intertwined trimer. The BpaA head is composed of structural elements that have been observed in other TAA head
The work was undertaken to expand the tools available for researching Burkholderia pseudomallei (Bp), the etiological agent of the tropical disease melioidosis. Melioidosis has the potential to pose a severe threat to public health and safety. In the United States, Bp is listed as a Tier-1 select agent by the Centers for Disease Control and Prevention (CDC), thus requiring high levels of regulation and biosafety level 3 (BSL3) facilities for experimental manipulation of live organisms. An avirulent ∆purM derivative of strain 1026b (Bp82) has proven to be a valuable tool for biosafe research as a select-agent excluded strain, but the high level of genetic diversity between Bp strains necessitates an expansion of the biosafe toolset. The ∆purM mutation was recapitulated in the Bp 576a strain, a serotype B background. An important difference between strains 1026b and 576a is the lipopolysaccharide (LPS), a major virulence factor and protective antigen. Polyclonal sera from 1026b-challenged non-human
We evaluated the correlation of Burkholderia pseudomallei quantities in blood versus urine, sputum or pus. Correlations between bacterial counts in blood and other samples were not found. It is likely that an initial seeding event to extracellular organs is followed by independent growth of B. pseudomallei, and that bacteria in the urine were not passively filtered from the bloodstream.
Background: Although melioidosis in endemic regions is usually caused by a diverse range of Burkholderia pseudomallei strains, clonal outbreaks from contaminated potable water have been described. Furthermore B. pseudomallei is classified as a CDC Group B bioterrorism agent. Ribotyping, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) have been used to identify genetically related B. pseudomallei isolates, but they are time consuming and technically challenging for many laboratories ...
Burkholderia pseudomallei is a gram-negative soil bacterium that is able to infect both humans and animals. Although cellculture based studies have revealed si...
The reaction of the catalase-peroxidase of Burkholderia pseudomallei with peroxyacetic acid has been analyzed using stopped-flow spectrophotometry. Two well-defined species were observed, the first de
Recently MALDI-TOF mass spectrum analysis has been considered an easy and discriminatory tool for identification of bacterial species (Lista et al., 2011). The results of MALDI-TOF mass spectrum analysis of the eight suspected isolates matched with that of the 16S rDNA sequence analysis. The four isolates DRDEBPS1001, DRDEBPS1002, DRDEBPS1003 and DRDEBPS1004 were confirmed as B. pseudomallei on the basis of score values 2.601, 2.099, 2.362 and 2.047. The other four isolates had been biochemically suspected but not supported by both the PCRs were also identified by MALDI-TOF spectrum analysis as Cupriavidus necator and Enterobacter cloacae with score value of 2.112, 2.122 and 2.341, 2.241 respectively, confirming the results of the 16S analysis.. The isolation of B. pseudomallei from soil is very complex as the presence of large numbers of closely related soil microflora interferes with its recovery although use of Ashdown broth and agar for the isolation of B. pseudomallei from soil samples ...
The overall goal of this project is to forensically characterize 100 unknown Burkholderia isolates in the US-Australia collaboration. We will identify genome-wide single nucleotide polymorphisms (SNPs) from B. pseudomallei and near neighbor species including B. mallei, B. thailandensis and B. oklahomensis. We will design microarray probes to detect these SNP markers and analyze 100 Burkholderia genomic DNAs extracted from environmental, clinical and near neighbor isolates from Australian collaborators on the Burkholderia SNP microarray. We will analyze the microarray genotyping results to characterize the genetic diversity of these new isolates and triage the samples for whole genome sequencing. In this interim report, we described the SNP analysis and the microarray probe design for the Burkholderia SNP microarray. ...
CUBRC, Inc. two weeks ago announced that CUBRCs Biological and Medical Sciences team, in collaboration with EpiVax, Inc., has received a four-year grant worth $1.87 million from the Defense Threat Reduction Agency (DTRA) within the Department of Defense (DoD). CUBRC, EpiVax, and scientists at the University of Florida will be investigating immune cells from patients that were previously infected with Burkholderiapseudomallei to understand how this bacterium evades the human immune system and use that information to engineer an effective vaccine. CUBRCs president and CEO, Tom McMahon, stated, "We are truly thankful for DTRAs confidence in our proposal to research vaccine antigens for prevention of Burkholderia pseudomallei infections, an important pathogen of biothreat potential.". CUBRC says it will leverage its technical expertise in biomedical R&D and its experience leading large federal government grants and contracts in collaboration with EpiVax and the University of Florida to execute ...
Burkholderia pseudomallei is an intracellular pathogen and the causative agent of melioidosis, a life-threatening disease of humans. Within host cells, superoxide is an important mediator of pathogen killing. In this study, we have identified the B. pseudomallei K96243 sodC gene, shown that it has superoxide dismutase activity, and constructed an allelic deletion mutant of this gene. Compared with the wild-type, the mutant was more sensitive to killing by extracellular superoxide, but not to superoxide generated intracellularly. The sodC mutant showed a markedly decreased survival in J774A.1 mouse macrophages, and reduced numbers of bacteria were recovered from human polymorphonuclear neutrophils (PMNs) when compared with the wild-type. The numbers of wild-type or mutant bacteria recovered from human diabetic neutrophils were significantly lower than from normal human neutrophils. The sodC mutant was attenuated in BALB/c mice. Our results indicate that SodC plays a key role in the virulence of B.
Bossé, J. T., Li, Y., Angen, O., Weinert, L. A., Chaudhuri, R. R., Holden, M. T., Williamson, S. M., Maskell, D. J., Tucker, A. W., Wren, B. W., Rycroft, A. N., Langford, P. R., on behalf of the BRaDP1T consortium & Holden, M. Jul 2014 In : Journal of Clinical Microbiology. p. 2380-2385 6 p.. Research output: Contribution to journal › Article ...
Ubiquinone (UQ), also called coenzyme Q, and plastoquinone (PQ) are electron carriers in oxidative phosphorylation and photosynthesis, respectively. The quinoid nucleus of ubiquinone is derived from the shikimate pathway; 4-hydroxybenzoate is directly formed from chorismate in bacteria, while it can be formed from either chorismate or tyrosine in yeast. The following biosynthesis of terpenoid moiety involves reactions of prenylation, decarboxylation, and three hydroxylations alternating with three methylations. The order of these reactions are somewhat different between bacteria and yeast. Phylloquinone (vitamin K1), menaquinone (vitamin K2), and tocopherol (vitamin E) are fat-soluble vitamins. Phylloquinone is a compound present in all photosynthetic plants serving as a cofactor for photosystem I-mediated electron transport. Menaquinone is an obligatory component of the electron-transfer pathway in bacteria ...
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adenylate kinase (Adk):In all organisms adenylate kinases (Adks) play a vital role in cellular energy metabolism and nucleic acid synthesis. Due to differences in catalytic properties between the Adks found in prokaryotes and in the cytoplasm of eukaryotes, there is interest in targeting this enzyme for new drug therapies against infectious bacterial agents. We present here the three-dimensional protein structure of Burkholderia pseudomallei Adk. This pathogen is an aerobic, Gram-negative, soil-dwelling bacterium responsible for the infectious disease melioidosis, which is a serious health problem in Northern Australia and Southeast Asia and accounts for 40% of all sepsis-related mortality in northeast Thailand. Due to its potential use in biological warfare and biological terrorism, B. pseudomallei is also of concern to the U.S. Centers for Disease Control and Prevention. An unexpected feature of the BpAdk crystal structure was the observation of two significantly different conformations of the protein
Development and validation of |i|Burkholderia pseudomallei|/i|-specific real-time PCR assays for clinical, environmental or forensic detection applications
Lowe, W., J.K. March, A.J. Bunnell, K.L. ONeill, and R.A. Robison. 2013. PCR-based methodologies used to detect and differentiate the Burkholderia pseudomallei complex: B. pseudomallei, B. mallei, and B. thailandensis. Current Issues in Molecular Biology 16: 23-54.. Gunnell, M.K., Adams, B.J., and Robison, R.A. 2015. The genetic diversity and evolution of Francisella tularensis with comments on detection by PCR. Current Issues in Molecular Biology. 18: 79-92.. Gunnell, M.K., Robison, R.A., and Adams, B.J. 2016. Natural selection in virulence genes of Francisella tularensis. Journal of Molecular Evolution. DOI 10.1007/s00239-016-9743-y, 1-15.. Lowe, C-W, Satterfield, B.A., Nelson, D.B., Thiriot, J.D., Heder, M.J., March, J.K., Drake, D.S., Lew, C.S., Bunnell, A.J., Moore, E.S., ONeill, K.L., and Robison, R.A. 2016. A quaduplex real-time assay for the rapid detection and differentiation of the most relevant members of the B. pseudomallei complex: B. mallei, B. pseudomallei, and B. thailandensis. ...
Based on these results, we conclude Selleck Ro 61-8048 that BoaA is a well-conserved gene product shared by B. mallei and B. pseudomallei. Table 2 Percent identity shared by boaA and boaB gene products BoaA (Bm ATCC23344) BoaA (Bm NCTC10247) BoaA (Bp K96243) BoaA (Bp DD503) BoaA (Bp 1710b) BoaB (Bp K96243) BoaB (Bp DD503) BoaB (Bp 1710b) BoaA (Bm ATCC23344) 100 BoaA (Bm NCTC10247) 86.9 100 BoaA (Bp K96243) 92.7 89.2 100 BoaA (Bp DD503) 94.4 82.2 90.6 100 BoaA (Bp 1710b) 90.4 83.1 92.4 93.6 100 BoaB (Bp K96243) 64 60 65 63.9 63.9 100 BoaB (Bp. DD503) 62 60.8 62.9 61.9 62.2 96.7 100 BoaB (Bp 1710b) 62.2 60.9 63.2 62.1 62.4 97 99.7 100 Bm = B. mallei Bp = B. pseudomallei Identification of a B. pseudomallei-specific gene encoding a putative autotransporter adhesin that resembles BoaA Further analysis of the annotated genomic sequence of B. pseudomallei K96243 identified the ORF locus tag number BPSL1705 as specifying a second Oca-like protein that is ~60% identical to BoaA. The last 776 aa of ...
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ID DDL_BURP0 Reviewed; 312 AA. AC A3NZL3; DT 10-JUN-2008, integrated into UniProtKB/Swiss-Prot. DT 03-APR-2007, sequence version 1. DT 22-NOV-2017, entry version 75. DE RecName: Full=D-alanine--D-alanine ligase {ECO:0000255,HAMAP-Rule:MF_00047}; DE EC=6.3.2.4 {ECO:0000255,HAMAP-Rule:MF_00047}; DE AltName: Full=D-Ala-D-Ala ligase {ECO:0000255,HAMAP-Rule:MF_00047}; DE AltName: Full=D-alanylalanine synthetase {ECO:0000255,HAMAP-Rule:MF_00047}; GN Name=ddl {ECO:0000255,HAMAP-Rule:MF_00047}; GN OrderedLocusNames=BURPS1106A_3548; OS Burkholderia pseudomallei (strain 1106a). OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; OC Burkholderiaceae; Burkholderia; pseudomallei group. OX NCBI_TaxID=357348; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=1106a; RA DeShazer D., Woods D.E., Nierman W.C.; RL Submitted (FEB-2007) to the EMBL/GenBank/DDBJ databases. CC -!- FUNCTION: Cell wall formation. {ECO:0000255,HAMAP-Rule:MF_00047}. CC -!- CATALYTIC ACTIVITY: ATP + 2 ...
Use this form (H3) for serodiagnosis of staphylococcal, streptococcal, Pseudomonas aeruginosa and Burkholderia pseudomallei antibodies.
Respiratory infections pose a threat to the health of the population worldwide. Within the RTG 1870 "Bacterial Respiratory Infections - Common and Specific Mechanisms of Pathogen Adaptation and Immune Defence" (BacRes) scientists of the University Greifswald carry out research projects on three versatile respiratory pathogens: Streptococcus pneumoniae (pneumococci), Staphylococcus aureus and Burkholderia pseudomallei. The bacteria can harmlessly colonize the nasopharynx, but also cause serious respiratory and invasive infections whose therapy poses huge problems, not least because of frequent antibiotic resistance of these pathogens. A better understanding of the potential of these bacteria to adapt to different environments in the human host and to evade the immune defense is urgently needed in order to develop novel therapeutic strategies and effective vaccines. This requires interdisciplinary research approaches and state-of-the-art experimental methods. Scientists of the UMG from infection ...
How can you expect a diabetic patient presented with mild fever and cough, very stable, able to walk, make jokes, laugh... like a mild infection, to end up in ICU and died within a day. I remember that patient was diagnosed as pneumonia based on symptoms and chest X-ray finding. Just mild perihilar hazziness. Admitted and started on Augmentin. A day after that, patient desaturate, intubated and was sent to ICU. That patient died after 1 day in ICU. 3 days later, blood cultures result came out to be Burkholderia pseudomallei. Its too late ...
Despite the pilgrims low bone signs of leprosy, the researchers were able to isolate M. leprae DNA from him for analysis. They first screened all three skeletons for signs of M. leprae using the multi-copy element RLEP, which they then confirmed by real-time PCR analysis of the multi-copy element IS1081. Only the pilgrim came back positive for M. leprae. The samples were also screened to determine whether they were infected with Brucella, Treponema pallidum, Burkholderia pseudomallei, Leishmania, Plasmodium, or hepatitis B virus, but none were. ...
The primary PhyloTrac window displays a taxonomic tree of the OTUs detected by the PhyloChip microarray, with mean intensities for each detected OTU displayed as a heat map of samples at the leaves of the tree. The user may dynamically filter the tree to hide low-abundance or borderline OTUs below user-specified intensity or PF thresholds, search and filter by keyword or clade, or summarize the analysis at any level of the tree from phylum to species. In Fig. 1a, the tree has been filtered to display the abundance of 19 select pathogenic or near-neighbor OTUs in San Antonio throughout the 17 weeks. In accordance with reference 2, the regular presence of phylogenetic near-neighbors to Bacillus anthracis, Burkholderia pseudomallei, and Clostridium botulinum is evident. In a second synchronized window, these OTUs are displayed in a time series plot, displaying their change in abundance over time, highlighted in yellow, in relation to all other OTUs, colored blue, and showing a spike in abundance in ...
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Looking for Pseudomonas pseudomallei? Find out information about Pseudomonas pseudomallei. A bacteria that is the causative agent of melioidosis, an endemic glanders-like disease of humans and animals that occurs most frequently in southeastern... Explanation of Pseudomonas pseudomallei
Abstract Melioidosis is a tropical disease of high mortality caused by the environmental bacterium, Burkholderia pseudomallei. We have collected clinical isolates from the highly endemic Northern Territory of Australia routinely since 1989, and animal and environmental B. pseudomallei isolates since 1991. Here we provide a complete record of all B. pseudomallei multilocus sequence types (STs) found in the Northern Territory to date, and distribution maps of the eight most common environmental STs. We observed surprisingly restricted geographic distributions of STs, which is contrary to previous reports suggesting widespread environmental dissemination of this bacterium. Our data suggest that B. pseudomallei from soil and water does not frequently disperse long distances following severe weather events or by migration of infected animals.
Endemic melioidosis is caused by genetically diverse Burkholderia pseudomallei strains. However, clonal outbreaks (multiple cases caused by 1 strain) have occurred, such as from contaminated potable water. B. pseudomallei is designated a group B bioterrorism agent, which necessitates rapidly recognizing point-source outbreaks. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) can identify genetically related isolates, but results take several days to obtain. We developed a simplified 4-locus multilocus variable number tandem repeat analysis (MLVA-4) for rapid typing and compared results with PFGE and MLST for a large number of well-characterized B. pseudomallei isolates. MLVA-4 compared favorably with MLST and PFGE for the same isolates; it discriminated between 65 multilocus sequence types and showed relatedness between epidemiologically linked isolates from outbreak clusters and between isolates from individual patients. MLVA-4 can establish or refute that a clonal ...
The etiologic agent of human and animal melioidosis is Burkholderia pseudomallei (5, 17). Both B. pseudomallei and its close species relative Burkholderia mallei are microbes recognized as having the potential for misuse as biological weapons (1). Typically, signs of pneumonia in patients with clinical melioidosis are a secondary result of sepsis (5). Presumably, victims suffering from a biological attack that employed B. pseudomallei or B. mallei would present clinically with pneumonic disease as the initial aspect. In order to develop a vaccine program for defending U.S. military forces against a potential B. mallei or B. pseudomallei attack, whole-body aerosol laboratory models of disease were established. With these murine melioidosis models, future vaccine candidates can be evaluated and tested for efficacy.. B. pseudomallei strain 1026b was selected because of its ease of genetic manipulation, its ability to replace genes, and its previously characterized virulence (6, 17). The 50% lethal ...
Melioidosis, caused by the highly recombinogenic bacterium Burkholderia pseudomallei, is a disease with high mortality. Tracing the origin of melioidosis outbreaks and understanding how the bacterium spreads and persists in the environment are essential to protecting public and veterinary health and reducing mortality associated with outbreaks. We used whole-genome sequencing to compare isolates from a historical quarter-century outbreak that occurred between 1966 and 1991 in the Avon Valley, Western Australia, a region far outside the known range of B. pseudomallei endemicity. All Avon Valley outbreak isolates shared the same multilocus sequence type (ST-284), which has not been identified outside this region. We found substantial genetic diversity among isolates based on a comparison of genome-wide variants, with no clear correlation between genotypes and temporal, geographical or source data. We observed little evidence of recombination in the outbreak strains, indicating that genetic ...
Melioidosis, caused by the highly recombinogenic bacterium Burkholderia pseudomallei, is a disease with high mortality. Tracing the origin of melioidosis outbreaks and understanding how the bacterium spreads and persists in the environment are essential to protecting public and veterinary health and reducing mortality associated with outbreaks. We used whole-genome sequencing to compare isolates from a historical quarter-century outbreak that occurred between 1966 and 1991 in the Avon Valley, Western Australia, a region far outside the known range of B. pseudomallei endemicity. All Avon Valley outbreak isolates shared the same multilocus sequence type (ST-284), which has not been identified outside this region. We found substantial genetic diversity among isolates based on a comparison of genome-wide variants, with no clear correlation between genotypes and temporal, geographical or source data. We observed little evidence of recombination in the outbreak strains, indicating that genetic ...
Burkholderia pseudomallei, the causative agent of melioidosis, is endemic to Southeast Asia and northern Australia. Clinical manifestations of disease are diverse, ranging from chronic infection to acute septicaemia. The current gold standard of diagnosis involves bacterial culture and identification which is time consuming and often too late for early medical intervention. Hence, rapid diagnosis of melioidosis is crucial for the successful management of melioidosis. The study evaluated 4 purified B. pseudomallei recombinant proteins (TssD-5, Omp3, smBpF4 and Omp85) as potential diagnostic agents for melioidosis. A total of 68 sera samples from Malaysian melioidosis patients were screened for the presence of specific antibodies towards these proteins using enzyme-linked immunosorbent assay (ELISA). Sera from patients with various bacterial and viral infections but negative for B. pseudomallei, as well as sera from healthy individuals, were also included as non-melioidosis controls. The Mann Whitney
Melioidosis is a tropical disease caused by the bacterium Burkholderia pseudomallei. Outbreaks are uncommon and can generally be attributed to a single point source and strain. We used whole-genome sequencing to analyse B. pseudomallei isolates collected from an historical 2-year long case cluster that occurred in a remote northern Australian indigenous island community, where infections were previously linked to a contaminated communal water supply. We analysed the genome-wide relatedness of the two most common multilocus sequence types (STs) involved in the outbreak, STs 125 and 126. This analysis showed that although these STs were closely related on a whole-genome level, they demonstrated evidence of multiple recombination events that were unlikely to have occurred over the timeframe of the outbreak. Based on epidemiological and genetic data, we also identified two additional patients not previously associated with this outbreak. Our results confirm the previous hypothesis that a single ...
This report published in Communicable Diseases Intelligence Volume 27, No 2, June 2003 describes the epidemiology of melioidosis, which is caused by the Gram negative bacterium Burkholderia pseudomallei and is endemic in northern Australia.
The bacterium Burkholderia ubonensis is commonly co-isolated from environmental specimens harbouring the melioidosis pathogen, Burkholderia pseudomallei. B. ubonensis has been reported in northern Australia and Thailand but not North America, suggesting similar geographic distribution to B. pseudomallei. Unlike most other Burkholderia cepacia complex (Bcc) species, B. ubonensis is considered non-pathogenic, although its virulence potential has not been tested. Antibiotic resistance in B. ubonensis, particularly towards drugs used to treat the most severe B. pseudomallei infections, has also been poorly characterised. This study examined the population biology of B. ubonensis, and includes the first reported isolates from the Caribbean. Phylogenomic analysis of 264 B. ubonensis genomes identified distinct clades that corresponded with geographic origin, similar to B. pseudomallei. A small proportion (4%) of strains lacked the 920kb chromosome III replicon, with discordance of presence/absence amongst
Evidence is well documented that Pseudomonas pseudomallei, the cause of melioidosis in animals and man, is a soil organism that occurs mainly in tropical and sub-tropical climates (Cambon 1955: Forunier 1965; Jananetra et al 1974). This letter is to report the idolation of 9 strains of Ps. pseudomallei from the soil and 3 strains from muddy water that drained into artifically-made holes in a 5 ha sheep paddock at the Animal Health Station, Oonoonba, where natural infections occur yearly during the wet season. The morphological, cultural and biochemical characteristics of the isolates agreed with those set down by Cottew (1950) and Laws (1964).. ...
Melioidosis is caused by the Gram-negative bacillus Burkholderia pseudomallei. Most clinical reports of disease are from south-east Asia and northern Australia. The organism is intrinsically resistant to most commonly available antibiotics. Standard therapy includes ceftazidime either alone or in combination with co-trimoxazole. The clinical advantage in adding cotrimoxazole has never been determined; nor has the activity of newer, fourth-generation cephalosporins, such as cefepime, been studied in the treatment of this condition. BALB/c mice have been shown to represent an animal model of melioidosis. This animal model was used in this study to compare the efficacy of ceftazidime and cefepime alone or with co-trimoxazole, in the therapy of melioidosis. Antibiotic levels in the mice were determined by HPLC, and dosing was modified to keep plasma antibiotic levels at or above the MIC for the organism-antibiotic combination for a significant part of a 12 h period. Bacterial load, as determined by ...
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Burkholderia thailandensis is a non-pathogenic environmental saprophyte closely related to Burkholderia pseudomallei, the causative agent of the often fatal animal and human disease melioidosis. To study B. thailandensis genomic variation, we profiled 50 isolates using a pan-genome microarray comprising genomic elements from 28 Burkholderia strains and species. Of 39 genomic regions variably present across the B. thailandensis strains, 13 regions corresponded to known genomic islands, while 26 regions were novel. Variant B. thailandensis isolates exhibited isolated acquisition of a capsular polysaccharide biosynthesis gene cluster (B. pseudomallei-like capsular polysaccharide) closely resembling a similar cluster in B. pseudomallei that is essential for virulence in mammals; presence of this cluster was confirmed by whole genome sequencing of a representative variant strain (B. thailandensis E555). Both whole-genome microarray and multi-locus sequence typing analysis revealed that the variant strains
Yap, E.H.,Thong, T.W.,Tan, A.L.,Yeo, M.,Tan, H.C.,Loh, H.,Teo, T.P.,Thong, K.T.,Singh, M.,Chan, Y.C. (1995). Comparison of Pseudomonas pseudomallei from humans, animals, soil and water by restriction endonuclease analysis.. Singapore Medical Journal 36 (1) : 60-62. [email protected] Repository ...
TY - CHAP. T1 - Management of patients with severe melioidosis in intensive care. AU - West, T Eoin. AU - Cheng, Allen. PY - 2012. Y1 - 2012. N2 - Melioidosis frequently presents as severe sepsis or septic shock. Appropriate managementtherefore dictates familiarity with standard sepsis therapies and approaches to intensive care. These treatments include early and aggressive haemodynamic management using intravenous fluids, vasopressors, and inotropes in a goal-directed fashion to maximise oxygen delivery to peripheral tissues. In the critically ill patient, oxygen consumption can be reduced with antipyretics, sedation, and mechanical ventilation. The role of glycaemic control and adjunctive treatments such as corticosteroids, activated protein C and granulocyte colony stimulating factor (G-CSF) are controversial. G-CSF has been formally studied in melioidosis patients, but the level of evidence for adjunctive treatments is insufficient. Mechanical ventilation for respiratory failure or acute ...
Health officials are stepping up public education and public hygiene campaigns advising people about boiling water, wearing boots in flood waters, etc.. For your information: Massey University researchers produce Leptospirosis video series. Melioidosis (also known as Whitmore disease and Nightcliff gardeners disease) is caused by the bacterium, Burkholderia pseudomallei. The disease though somewhat rare has been seen in areas of Southeast Asia and Northern Australia, particularly after heavy rains. In Thailand it is considered a disease of rice farmers.. The organism is saprophytically found in soil and water. People usually get infected by contact with contaminated soil or water through skin wounds, inhalation or rarely through ingestion of contaminated water.. Person to person transmission can occur through contact with blood and body fluids of an infected person.. Depending on how heavy the infection incubation can range from hours to weeks. Infection may show no symptoms but it can quickly ...
2,3-bisphosphoglycerate-dependent phosphoglycerate mutase from Burkholderia pseudomallei: We present here an ensemble of structures solved by the Seattle Structural Genomics Center for Infectious Disease (SSGCID) of 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase, or PGAM, from Burkholderia pseudomallei, a pathogen which causes the serious skin infection melioidosis.
The worldwide epidemiology of melioidosis is changing. We describe a case of acute melioidosis in Spain in a patient who had traveled to Africa. A novel sequence type of Burkholderia pseudomallei was identified in this patient. Clinicians should be a ...
Melioidosis is diagnosed by isolating Burkholderia pseudomallei from blood, urine, sputum, skin lesions, or abscesses; or by detecting an antibody response to the bacteria.
may be the etiological agent of human melioidosis, a disease with a broad spectrum of clinical manifestations ranging from fatal septicemia to chronic localized infection or asymptomatic latent infection. interferon (IFN-), interleukin-6 (IL-6), monocyte JIB-04 chemotactic protein-1 (MCP-1), and tumor necrosis factor- (TNF-) were induced during chronic infection, and histopathological analysis showed features in common with human melioidosis. Interestingly, many of these features were similar to those induced by in humans, such as development of a collagen cord that encapsulates the lesions, the presence of multinucleated giant cells, and granulomas with a caseous necrotic center, which may explain why chronic melioidosis is often misdiagnosed as tuberculosis. Our model now provides a relevant and practical tool to define the immunological features of chronic melioidosis and aid in the development of more effective treatment of this disease in humans. is a Gram-negative soil bacterium that is ...
A simple method was developed for detection and differentiation of five Tier 1 bacterial agents, including Bacillus anthracis, Francisella tularensis, Yersinia pestis, Burkholderia pseudomallei and Burkholderia mallei as well as their closely related near neighbors by gas chromatography-mass spectrometry (GC-MS). Generally, different classes of compounds can be used as biomarkers for biowarfare agent detection, including nucleic acids (i.e., DNA or RNA), proteins (i.e., antibodies), carbohydrates (i.e., sugars), lipopolysaccharides, lipids (i.e., fatty acids) and small molecules. One-step thermochemolysis (TCM) was developed to provide GC-MS detectable biomarker signatures, including sugars, fatty acids and small molecules. Solid phase micro-extraction (SPME) was used for biomarker extraction, concentration and introduction into the GC-MS. Statistical algorithms were constructed using a combination of biomarkers for the five agents, which were robust against different growth conditions (medium and
A polyphasic taxonomic study including DNA-DNA reassociation experiments and an extensive biochemical characterization was performed on 14 Burkholderia isolates from moss gametophytes of nutrient-poor plant communities on the southern Baltic Sea coast in northern Germany. The strains were classified within two novel species, for which the names Burkholderia bryophila sp. nov. and Burkholderia megapolitana sp. nov. are proposed. The former species also includes isolates from grassland and agricultural soil collected in previous studies. Strains Burkholderia bryophila 1S18T (=LMG 23644T =CCUG 52993T) and Burkholderia megapolitana A3T (=LMG 23650T =CCUG 53006T) are the proposed type strains. They were isolated from Sphagnum rubellum and Aulacomnium palustre, respectively, growing in the Ribnitzer Großes Moor nature reserve (Mecklenburg-Pommern, Germany). All moss isolates of both novel species showed antifungal activity against phytopathogens as well as plant-growth-promoting properties.
A definitive diagnosis is made by culturing the organism from any clinical sample, because the organism is never part of the normal human flora.. A definite history of contact with soil may not be elicited, as melioidosis can be dormant for many years before manifesting. Attention should be paid to a history of travel to endemic areas in returned travellers. Some authors recommend considering possibility of melioidosis in every febrile patient with a history of traveling to and/or staying at endemic areas.. A complete screen (blood culture, sputum culture, urine culture, throat swab, and culture of any aspirated pus) should be performed on all patients with suspected melioidosis (culture on blood agar as well as Ashdowns medium). A definitive diagnosis is made by growing B. pseudomallei from any site. A throat swab is not sensitive, but is 100% specific if positive, and compares favourably with sputum culture. The sensitivity of urine culture is increased if a centrifuged specimen is cultured, ...
Khor, W.C. and Puah, S.M. and Tan, J.A.M.A and Puthucheary, S.D. and Chua, K.H. (2015) Phenotypic and genetic diversity of Aeromonas Species isolated from fresh water lakes in Malaysia. PLoS ONE, 10 (12). ISSN 1932-6203 Karunakaran, R. and Sun, T.T. and Rahim, F.F. and Bee, B.L. and Sam, I.C. and Kahar-Bador, M. and Hassan, H. and Puthucheary, S.D. (2012) Ceftriaxone resistance and genes encoding extended-spectrum ß-lactamase among non-typhoidal salmonella species from a tertiary care hospital in Kuala Lumpur, Malaysia. Japanese Journal of Infectious Diseases, 65. ISSN 1344-6304 Liew, S.M. and Tay, S.T. and Wongratanacheewin, S. and Puthucheary, S.D. (2012) Enzymatic profiling of clinical and environmental isolates of Burkholderia pseudomallei. Tropical Biomedicine, 29 (1). pp. 160-168. ISSN 0127-5720 Puthucheary, S.D. and Puah, S.M. and Chua, K.H. (2012) Molecular characterization of clinical isolates of Aeromonas species from Malaysia. PLoS ONE, 7 (2). ISSN 1932-6203 Puthucheary, S.D. and ...
Looking for Whitmore's disease? Find out information about Whitmore's disease. An endemic bacterial disease, primarily of rodents but occasionally communicable to humans, caused by Pseudomonas pseudomallei and characterized by... Explanation of Whitmore's disease
TUESDAY, July 7, 2020 (HealthDay News) -- A potentially deadly antibiotic-resistant bacteria could be hiding in the dirt and water of the southernmost U.S. states, warns a new report from the U.S. Centers for Disease Control and Prevention.. The bacterial infection, called melioidosis, caused the lungs of a 63-year-old Texan to shut down in late 2018, forcing doctors to put him on a ventilator to save his life, the researchers said.. U.S. citizens whove caught melioidosis in the past typically picked it up in a foreign country, but this man had not recently traveled abroad, said Johanna Salzer, a veterinary medical officer with the CDCs Bacterial Special Pathogens Branch.. Whats more, the bacteria that caused the mans melioidosis was genetically similar to two prior U.S. cases, one in Texas in 2004 and one in Arizona in 1999.. "We feel like this is evidence that it could be in the environment" in the United States, Salzer said. "We just need to find it.". Melioidosis is caused by the ...
Learn about the veterinary topic of Overview of Melioidosis. Find specific details on this topic and related topics from the Merck Vet Manual.
Lineage: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Burkholderia; pseudomallei group; Burkholderia ...
We have to understand the risks, mitigate against them and seek a balance that works for all.". Professor Mahenthiralingam, has studied burkholderia bacteria in cystic fibrosis for many years and the research led to two new human antibiotics , Cepacin A and B. But he and his team are now working to see if Cepacin would be effective in crop pests as well. Using genomic sequencing of the bacterial DNA the researchers could identify the genes for burkholderia to manufacture Cepacin.. "Burkholderia split their genomic DNA across 3 fragments, called replicons," said Professor Mahenthiralingam.. "We removed the smallest of these 3 replicons to create a mutant Burkholderia strain which, when tested on germinating peas, still demonstrated excellent biopesticidal properties.. Tests in mice susceptible to lung infections, intended to mimic the regular infections faced by people with cystic fibrosis found the mutant strain also did not cause them harm.. ...
Bacterial persistence, known as noninherited antibacterial resistance, is a factor contributing to the establishment of long-lasting chronic bacterial infections. In this study, we examined the ability of nicotinamide (NA) to potentiate the activity of different classes of antibiotics against Burkholderia thailandensis persister cells. ...
Either well-conducted animal studies show no risk of birth defects, and there are no well-done human studies, or animal studies suggest an increased risk, but human studies do not Examples: Zofran...
Before taking Prevacid, tell your doctor if you ever have liver disease. You may not be able to take Prevacid, or you may require a dosage adjustment or special monitoring during treatment. Prevacid is in the FDA pregnancy category B. This means that it is not expected to be harmful to an unborn baby. Do not take Prevacid without first talking to your doctor if you are pregnant. It is not known whether Prevacid passes into breast milk. Do not take Prevacid without first talking to your doctor if you are breast-feeding a baby ...
Before taking Prevacid, tell your doctor if you ever have liver disease. You may not be able to take Prevacid, or you may require a dosage adjustment or special monitoring during treatment. Prevacid is in the FDA pregnancy category B. This means that it is not expected to be harmful to an unborn baby. Do not take Prevacid without first talking to your doctor if you are pregnant. It is not known whether Prevacid passes into breast milk. Do not take Prevacid without first talking to your doctor if you are breast-feeding a baby ...
TY - JOUR. T1 - Thalassemia Major Is a Major Risk Factor for Pediatric Melioidosis in Kota Kinabalu, Sabah, Malaysia. AU - Fong, SM. AU - Wong, Ke. AU - Fukushima, Masako. AU - Yeo, Tsin. PY - 2015/6/15. Y1 - 2015/6/15. N2 - Background: Melioidosis is an important cause of community-acquired infection in Southeast Asia and northern Australia. Studies from endemic countries have demonstrated differences in the epidemiology and clinical features among children diagnosed with melioidosis. This suggests that local data are needed to determine the risk factors and outcome in specific areas.Methods: This was a retrospective study of all children admitted to Likas Womens and Children Hospital, Kota Kinabalu, Sabah, Malaysia, with a blood or clinical sample positive for Burkholderia pseudomallei from 2001 to 2012.Results: Of 28 children with confirmed melioidosis, 27 records were reviewed including 11 (41%) children with thalassemia major. Twenty of the children had bacteremia, and 16 (59%) had a fatal ...
Background. Recurrent melioidosis occurs in ∼6% of patients in the first year following the initial presentation. A recent study revealed that 25% of patients with recurrence had reinfection rather than a relapse resulting from a failure to cure. The aim of this study was to reevaluate these 2 patient groups to define their individual risk factors.. Methods. All adult patients who presented to Sappasithiprasong Hospital (Ubon Ratchathani, in northeast Thailand) with culture-confirmed melioidosis during the period 1986-2004 and who survived to receive oral antimicrobial therapy were observed until July 2005. Clinical factors and antimicrobial treatment of patients with recurrent disease due to relapse or reinfection, as confirmed by bacterial genotyping, were compared using a time-varying Cox proportional hazard model.. Results. Of 889 patients who survived and underwent follow-up, 86 patients (9.7%) presented with relapse, and 30 patients (3.4%) became reinfected. There was no difference in ...
Pathogenic Burkholderia rely on host factors for efficient intracellular replication and are highly refractory to antibiotic treatment. To identify host genes that are required by Burkholderia spp. during infection, we performed a RNA interference (RNAi) screen of the human kinome and identified 35 host kinases that facilitated Burkholderia thailandensis intracellular survival in human monocytic THP-1 cells. We validated a selection of host kinases using imaging flow cytometry to assess efficiency of B. thailandensis survival in the host upon siRNA-mediated knockdown. We focused on the role of the novel protein kinase C isoform, PKC-η, in Burkholderia infection and characterized PKC-η/MARCKS signaling as a key event that promotes the survival of unopsonized B. thailandensis CDC2721121 within host cells. While infection of lung epithelial cells with unopsonized Gram-negative bacteria stimulated phosphorylation of Ser175/160 in the MARCKS effector domain, siRNA-mediated knockdown of PKC-η ...
Pyogenic liver abscess is a common entity in Indian subcontinent and is mostly caused by gram negative bacteria. Melioidosis is not commonly seen in India and only a few cases are reported. It can give rise to multiple abscesses at different sites in
Wallqvist, A., H. Wang, N. Zavaljevski, V. Memisevic, K. Kwon, R. Pieper, S. V. Rajagopala, and J. Reifman. Mechanisms of action of Coxiella burnetii effectors inferred from host-pathogen protein interactions. PLOS ONE. 2017 November 27; 12(11):e0188071. [PDF]. Memisevic, V., K. Kumar, N. Zavaljevski, D. DeShazer, A. Wallqvist, and J. Reifman. DBSecSys 2.0: a database of Burkholderia mallei and Burkholderia pseudomallei secretion systems. BMC Bioinformatics. 2016 September 20; 17:387. [PDF]. Wallqvist, A., V. Memisevic, N. Zavaljevski, R. Pieper, S. V. Rajagopala, K. Kwon, C. Yu, T. A. Hoover, and J. Reifman. Using host-pathogen protein interactions to identify and characterize Francisella tularensis virulence factors. BMC Genomics. 2015 December 29; 16:1106. [PDF]. Chiang, C. Y., I. Uzoma, D. J. Lane, V. Memisevic, F. Alem, K. Yao, S. Bavari, A. Wallqvist, R. M. Hakami, and R. G. Panchal. A reverse-phase protein microarray-based screen identifies host signaling dynamics upon Burkholderia spp. ...
Burkholderia pseudomallei and Burkholderia mallei are highly pathogenic Gram - negative bacteria and the causative agents of melioidosis and glanders, respectively. These infections, which occur in humans and other animals, are endemic is wide regions of the developing world. This Phase II SBIR project will build on the success of our Phase I work, which established methods for generating monoclon .... ...
Dr. Gelhaus is Senior Program Manager in the Medical Countermeasures Division of MRIGlobal, where he directs cutting edge research to protect the world from biological threats.. Dr. Gelhaus is also an adjunct faculty member at KCU. Dr. Gelhaus has 19 years of experience in the field of immunology, covering a diverse range of topics. Dr. Gelhaus has nine years of experience in infectious disease, with emphasis on bioweapons and bioterror threats.. At the United States Army Research Institute of Infectious Diseases (USAMRIID), Dr. Gelhaus performed research on the role of toll-like receptors (TLR) involved in the pathogenesis of diseases caused by gram-negative select agent bacteria, namely, Burkholderia mallei, Burkholderia pseudomallei, Francisella tularensis and Yersinia pestis.. At Battelle, Dr. Gelhaus was involved in the development of animal models as a Study Director for Good Laboratory Practices regulated studies, including supporting licensure of medical countermeasures through the US ...
ID BURM7_2_PE1705 STANDARD; PRT; 480 AA. AC BURM7_2_PE1705; A3MMD6; DT 00-JAN-0000 (Rel. 1, Created) DT 00-JAN-0000 (Rel. 2, Last sequence update) DT 00-JAN-0000 (Rel. 3, Last annotation update) DE SubName: Full=Drug resistance transporter, EmrB/QacA subfamily; DE (BURM7_2.PE1705). GN OrderedLocusNames=BMA10247_1885; OS BURKHOLDERIA MALLEI NCTC 10247. OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; OC Burkholderiaceae; Burkholderia; pseudomallei group. OX NCBI_TaxID=320389; RN [0] RP -.; RG -.; RL -.; CC -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS BURM7_2.PE1705. CC Burkholderia mallei NCTC 10247 chromosome I, complete genome. CC sequence. CC -!- ANNOTATIONS ORIGIN:A3MMD6_BURM7 CC -!- GENE_FAMILY: HOG000239124 [ FAMILY / ALN / TREE ] DR UniProtKB/Swiss-Prot; A3MMD6; -. DR EMBL; CP000548; ABO05294.1; -; Genomic_DNA. DR RefSeq; YP_001081422.1; NC_009080.1. DR ProteinModelPortal; A3MMD6; -. DR STRING; A3MMD6; -. DR GeneID; 4893681; -. DR GenomeReviews; CP000548_GR; ...
ID BURMS_2_PE2987 STANDARD; PRT; 610 AA. AC BURMS_2_PE2987; A1V7Z1; DT 00-JAN-0000 (Rel. 1, Created) DT 00-JAN-0000 (Rel. 2, Last sequence update) DT 00-JAN-0000 (Rel. 3, Last annotation update) DE SubName: Full=Glucosamine--fructose-6-phosphate aminotransferase, DE isomerizing; EC=2.6.1 16; (BURMS_2.PE2987). GN Name=glmS-2; OrderedLocusNames=BMASAVP1_A3050; OS BURKHOLDERIA MALLEI SAVP1. OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; OC Burkholderiaceae; Burkholderia; pseudomallei group. OX NCBI_TaxID=320388; RN [0] RP -.; RG -.; RL -.; CC -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS BURMS_2.PE2987. CC Burkholderia mallei SAVP1 chromosome I, complete genome. CC sequence. CC -!- ANNOTATIONS ORIGIN:A1V7Z1_BURMS CC -!- GENE_FAMILY: HOG000258898 [ FAMILY / ALN / TREE ] DR UniProtKB/Swiss-Prot; A1V7Z1; -. DR EMBL; CP000526; ABM52310.1; -; Genomic_DNA. DR RefSeq; YP_994344.1; NC_008785.1. DR ProteinModelPortal; A1V7Z1; -. DR SMR; A1V7Z1; 2-610. DR STRING; A1V7Z1; -. DR ...
A review of case records for 1817 Thai patients with melioidosis revealed that ,10% of the 382 patients with diabetes mellitus were insulin dependent. This provides evidence against the hypothesis that insulin deficiency contributes to the known susceptibility to melioidosis in patients with diabetes mellitus.. ...
Hong-Geller E. (2013) Small-RNA-mediated regulation of host-pathogen interactions. Virulence. In preparation.. Stubben CJ, Micheva-Viteva S, Shou Y, Dunbar JM, and Hong-Geller E. (2013) Differential expression of small RNAs from Burkholderia thailandensis in response to environmental and stressful growth conditions. In preparation.. Shepherd DP, Li N, Micheva-Viteva S, Munsky B, Hong-Geller E, and Werner J. (2013) Counting small RNA in pathogenic bacteria. Submitted.. Micheva-Viteva S, Shou Y, Nowak-Lovato K, Rector KD, and Hong-Geller E. (2013) c-KIT-EGR1 signaling is targeted by Yersinia during infection. Submitted.. Nowak-Lovato K, Alexandrov LB, Banisadr A, Bauer AL, Bishop AR, Usheva A, Mu F, Hong-Geller E, Rasmussen K, Hlavacek WS, and Alexandrov BS. (2013) Binding of Nucleoid-associated Protein Fis to DNA is Regulated by DNA Breathing Dynamics. PLoS Comp. Biol. Jan;9(1):e1002881. doi: 10.1371/journal.pcbi.1002881.. Marti-Arbona R, Teshima M, Anderson PS, Nowak-Lovato K, Hong-Geller E, ...
Infections and inflammation can lead to cachexia and wasting of skeletal muscle and fat tissue by as yet poorly understood mechanisms. We observed that gut colonization of mice by a strain of Escherichia coli prevents wasting triggered by infections or physical damage to the intestine. During intestinal infection with the pathogen Salmonella Typhimurium or pneumonic infection with Burkholderia thailandensis, the presence of this E. coli did not alter changes in host metabolism, caloric uptake, or inflammation but instead sustained signaling of the insulin-like growth factor 1/phosphatidylinositol 3-kinase/AKT pathway in skeletal muscle, which is required for prevention of muscle wasting. This effect was dependent on engagement of the NLRC4 inflammasome. Therefore, this commensal promotes tolerance to diverse diseases. ...
Lineage: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Burkholderia; pseudomallei ...
Define glanders: a highly contagious and life-threatening disease of horses and other equines (such as donkeys and mules) or sometimes other animals …
Directed by David Nerlich, Andrew Traucki. With Diana Glenn, Maeve Dermody, Andy Rodoreda, Ben Oxenbould. A terrifying tale of survival in the mangrove swamps of Northern Australia
Willan R.C.(2001). Description of Pyrene morrisoni sp. nov.: a bulliform dove shell (Mollusca: Columbellidae) from Northern Australia. Vita Marina 47(4): 185-193 ...
Conflicting evidence exists on the prognostic value of PES in BS,4,14,15⇓⇓ and no other predictors of adverse outcome are available. A consensus exists on the indication of an ICD in cardiac arrest survivors,16,17⇓ yet treatment of the majority of patients with BS is undefined.. We have assessed in our population the validity of the stratification scheme proposed by Brugada et al,18 who divided patients into 4 groups, based on clinical features and inducibility at PES (categories A to D). We observed 1 of 14 events in category D (7% of cardiac arrest) and 3 of 35 events in category C (8% of cardiac arrest): Brugada et al18 recommend no ICD in category D and ICD implantation in category C; obviously, our data cannot endorse this recommendation. When category A and category B are compared, no differences are observed (33% of events in category B and 30% of events in category A).. We therefore explored the value of several clinical parameters to differentiate between patients with and without ...
Attie, There is a categorical system for use of drugs in pregnancy. The safest drugs are in Category A, followed (in order) by Category B, C, D and X. A good web site for this is...
This survey investigated the variation in the use of the breast core biopsy categories B1 normal and B2 benign. METHOD: A survey with case scenarios was circulated to 701 breast pathologists in the UK. RESULTS: The response ...
Burkholderia pseudomallei Meningitis multiple Meningococcal disease Neisseria meningitidis Metagonimiasis usually Metagonimus ...
For Burkholderia pseudomallei, some clinical antibiotic agents that are used to treat its infection, mellioidosis, can induce ... "Modified Virulence of Antibiotic-Induced Burkholderia pseudomallei Filaments". Antimicrobial Agents and Chemotherapy. 49 (3): ... However, the B.pseudomallei filaments revert to normal forms when the antibiotics are removed. Moreover, bacillary daughter ...
Colonies of Burkholderia pseudomallei, one of many pathogenic Betaproteobacteria. Scientific classification Domain: Bacteria ... Other members of the class can infect plants, such as Burkholderia cepacia which causes bulb rot in onions as well as ...
Brett PJ, DeShazer D, Woods DE (January 1998). "Burkholderia thailandensis sp. nov., a Burkholderia pseudomallei-like species ...
During infection, some bacteria (e.g., Burkholderia pseudomallei) therefore produce superoxide dismutase to protect themselves ... "Superoxide dismutase C is required for intracellular survival and virulence of Burkholderia pseudomallei". Microbiology. 157 ( ...
Colonies of Burkholderia pseudomallei on Müller-Hinton agar after 72 hours incubation. ...
Inglis, TJ; Chiang, D; Lee, GS; Chor-Kiang, L (1998). "Potential misidentification of Burkholderia pseudomallei by API 20NE". ... Care must be taken because Burkholderia pseudomallei is commonly misidentified as C. violaceum by many common identification ... "Comparison of Automated and Nonautomated Systems for Identification of Burkholderia pseudomallei". J Clin Microbiol. 40 (12): ... The two are readily distinguished because B. pseudomallei produces large wrinkled colonies, whereas C. violaceum produces a ...
"Structure of a Burkholderia pseudomallei trimeric autotransporter adhesin head". PLoS ONE. 5 (9): e12803. doi:10.1371/journal. ...
In Burkholderia pseudomallei BimA initiates actin polymerization in vitro. It is assumed that intracellular migration of this ...
Brett PJ, DeShazer D, Woods DE (January 1998). "Burkholderia thailandensis sp. nov., a Burkholderia pseudomallei-like species ...
Burkholderia pseudomallei Center for Biosecurity Agent Fact Sheet Burkholderia pseudomallei genomes and related information at ... and Burkholderia pseudomallei Selective Agar for Clinical Isolation of Burkholderia pseudomallei". Journal of Clinical ... 2011). "Burkholderia multivorans acts as an antagonist against the growth of Burkholderia pseudomallei in soil". Microbiology ... Many commercial kits for identifying bacteria may misidentify B. pseudomallei (see Burkholderia pseudomallei for a more ...
... and Burkholderia pseudomallei selective agar for clinical isolation of Burkholderia pseudomallei". J Clin Microbiol. 43 (10): ... Ashdown's medium is a selective culture medium for the isolation and characterisation of Burkholderia pseudomallei (the ... 2007). "Biological relevance of colony morphology and phenotypic switching by Burkholderia pseudomallei". J Bacteriol. 189 (3 ... The medium is also enriched with 4% glycerol, which is required by some strains of B. pseudomallei to grow. B. pseudomallei ...
Kim, M.S.; Shin, D.H. (2009). "A preliminary X-ray study of sedoheptulose-7-phosphate isomerase from Burkholderia pseudomallei ...
The first structure of a needle-complex monomer was NMR structure of BsaL from "Burkholderia pseudomallei" and later the ... the type III secretion needle protein of Burkholderia pseudomallei". Journal of Molecular Biology. 359 (2): 322-30. doi:10.1016 ... Burkholderia (glanders), Yersinia (plague), Chlamydia (sexually transmitted disease), Pseudomonas (infects humans, animals and ...
It includes some pathogenic species, such as Burkholderia mallei (glanders) and Burkholderia pseudomallei (melioidosis). ... to accommodate twelve species of the genera Burkholderia and Paraburkholderia". International Journal of Systematic and ... Dobritsa, AP; Samadpour, M (August 2016). "Transfer of eleven species of the genus Burkholderia to the genus Paraburkholderia ...
"Caspase-6 mediates resistance against Burkholderia pseudomallei infection and influences the expression of detrimental ...
Burkholderia cepacia Burkholderia mallei (glanders) Burkholderia pseudomallei (melioidosis) Chlamydia trachomatis (chlamydia) ...
Burkholderia pseudomallei (Q63NG7), Brucella abortus (Q57B94), Brucella suis (Q8FYS0) and Brucella melitensis (Q8YJ29). The ...
... expression in Burkholderia pseudomallei". Journal of Microbiology and Immunology. 57(9). CS1 maint: Explicit use of et al. ( ... pseudomallei, that rpoS regulates eight oxidative responsive proteins including ScoA (a SCOT subunit) not previously known for ... pseudomallei. RpoS-dependent genes involved in changes in cell membrane permeability and general cell morphology mostly belong ...
Burkholderia pseudomallei and Edwardsiella tarda are two other organisms which possess a T6SS that appears dedicated for ...
She is particularly known for her work on the bacterium Burkholderia pseudomallei and on Methicillin-resistant Staphylococcus ... infection mechanisms of Burkholderia pseudomallei and Melioidosis. Peacock has published around 150 papers on these topics. ...
A recent Australian study as shown that bacterium causing the tropical disease melioidosis, Burkholderia pseudomallei can also ... "Burkholderia pseudomallei penetrates the brain via destruction of the olfactory and trigeminal nerves: implications for the ...
Burkholderia pseudomallei. Meningitis. multiple. Meningococcal disease. Neisseria meningitidis. Metagonimiasis. usually ...
Caspase-11 has been shown to be activated by Burkholderia pseudomallei, Gram-negative bacteria found in the soil of southeast ...
KirBac1.1, from Burkholderia pseudomallei, is 333 amino acyl residues (aas) long with two N-terminal TMSs flanking a P-loop ( ...
Burkholderia pseudomallei various animals direct contact with contaminated soil and surface water ... Burkholderia mallei. horses, donkeys direct contact Gnathostomiasis Gnathostoma spp. dogs, minks, opossums, cats, lions, tigers ...
ABSTRACT: BACKGROUND: Burkholderia pseudomallei is a soil-dwelling saprophyte and the cause of melioidosis. Horizontal gene ... pseudomallei. In silico analysis of 10 B. pseudomallei genome sequences provided evidence for the variable presence of these ... Although there is some evidence for the variable distribution of genomic islands in B. pseudomallei isolates, little is known ... RESULTS: Five islands from B. pseudomallei strain K96243 were chosen as representatives of different types of genomic islands ...
Melioidosis caused by the environmental gram-negative bacillus Burkholderia pseudomallei, is classically characterized by ... New insights into Burkholderia pseudomallei infection (melioidosis). International Congress on Bacteriology & Infectious ... Melioidosis caused by the environmental gram-negative bacillus Burkholderia pseudomallei, is classically characterized by ... with a special interest Burkholderia pseudomallei and Salmonella typhi infection and the role of diabetes as well as the gut ...
Burkholderia pseudomallei. We have collected clinical isolates from the highly endemic Northern Territory of Australia ... Here we provide a complete record of all B. pseudomallei multilocus sequence types (STs) found in the Northern Territory to ... Our data suggest that B. pseudomallei from soil and water does not frequently disperse long distances following severe weather ... routinely since 1989, and animal and environmental B. pseudomallei isolates since 1991. ...
Compared with B. pseudomallei isolates having typical bimA alleles, isolates that contain the B. mallei-like variation ... Recently, we reported that a subset of clinical isolates of B. pseudomallei from Australia have heightened virulence and ... These findings highlight the implications of bimA variation on disease progression of B. pseudomallei infection and have ... Increased Neurotropic Threat from Burkholderia pseudomallei Strains with a B. mallei-like Variation in the bimA Motility Gene, ...
GDA involving β-lactamase may be a common ceftazidime resistance mechanism in B. pseudomallei. ... Ceftazidime is the antibiotic of choice for treatment of Burkholderia pseudomallei infections (melioidosis). The chromosomally ... Burkholderia pseudomallei acquired ceftazidime resistance due to gene duplication and amplification. Chirakul S., Somprasong N ... Infection with Burkholderia pseudomallei - immune correlates of survival in acute melioidosis * Community engagement for the ...
The interactions of Burkholderia species and Acanthamoeba castellanii, a species of free-living amoeba, were studied to better ... Optimal entry time into an amoeba trophozoite was found to be about three hours for all ten B. pseudomallei isolates. ... In this study, the adherence and persistence of several B. pseudomallei clinical isolates were compared to that of B. ... Results showed that B. pseudomallei isolates can enter amoeba and survive therein at varying levels of efficiency. Some ...
Epidemiological tracking and population assignment of the non-clonal bacterium, burkholderia pseudomallei. In: PLoS Neglected ... Epidemiological tracking and population assignment of the non-clonal bacterium, burkholderia pseudomallei. / Dale, Julia; Price ... Epidemiological tracking and population assignment of the non-clonal bacterium, burkholderia pseudomallei. PLoS Neglected ... For non-clonal species such as Burkholderia pseudomallei, however, shared allelic states between distantly related isolates ...
Within-host evolution of Burkholderia pseudomallei during chronic infection of seven Australasian cystic fibrosis patients. ... Within-host evolution of Burkholderia pseudomallei during chronic infection of seven Australasian cystic fibrosis patients ... Within-host evolution of Burkholderia pseudomallei during chronic infection of seven Australasian cystic fibrosis patients ... Within-host evolution of Burkholderia pseudomallei during chronic infection of seven Australasian cystic fibrosis patients. ...
Burkholderia pseudomallei Antibodies in Individuals Living in Endemic Regions in Northeastern Brazil. D.B. Rolim, D.C.F.L. ... Burkholderia pseudomallei Antibodies in Individuals Living in Endemic Regions in Northeastern Brazil. / Rolim, D.B.; Vilar, D.C ... Burkholderia pseudomallei Antibodies in Individuals Living in Endemic Regions in Northeastern Brazil. American Journal of ... title = "Burkholderia pseudomallei Antibodies in Individuals Living in Endemic Regions in Northeastern Brazil", ...
... and Burkholderia pseudomallei selective agar for clinical isolation of Burkholderia pseudomallei". Journal of Clinical ... Burkholderia pseudomallei (also known as Pseudomonas pseudomallei) is a Gram-negative, bipolar, aerobic, motile rod-shaped ... 2011). "Rapid identification of Burkholderia pseudomallei and Burkholderia mallei by fluorescence in situ hybridization (FISH) ... Pathema Burkholderia resource "Burkholderia pseudomallei". NCBI Taxonomy Browser. 28450. ...
Burkholderia pseudomallei in Southwestern United States A recently published case of melioidosis in a Texas resident has ... Contact your state LRN for assistance with rule-out of pseudomallei.. For more information, visit CDCs melioidosis website or ... Laboratory staff who may have been exposed to B. pseudomallei should refer to existing CDC guidance. For information about ... increased concerns that Burkholderia pseudomallei, the bacteria that causes this rare disease, may be present in soil and water ...
Laboratory Exposure to Burkholderia pseudomallei --- Los Angeles, California, 2003. On July 26, 2003, the Los Angeles County ... Potential misidentification of Burkholderia pseudomallei by API 20NE. Pathology 1998;30:62--4. ... Burkholderia pseudomallei, a category B biologic terrorism agent and the causative organism for melioidosis, which is endemic ... pseudomallei, which causes melioidosis infection. The majority of infections with B. pseudomallei are asymptomatic (1). ...
Burkholderia pseudomallei str. MSHR346. › Burkholderia pseudomallei strain MSHR346. Rank i. -. Lineage i. › cellular organisms ...
Burkholderia pseudomallei: Brief Summary provided by wikipedia EN Burkholderia pseudomallei (also known as Pseudomonas ... and Burkholderia pseudomallei selective agar for clinical isolation of Burkholderia pseudomallei. Journal of Clinical ... Burkholderia pseudomallei (also known as Pseudomonas pseudomallei) is a Gram-negative, bipolar, aerobic, motile rod-shaped ... 2011). Rapid identification of Burkholderia pseudomallei and Burkholderia mallei by fluorescence in situ hybridization (FISH) ...
DUF1929 domain-containing protein [Burkholderia pseudomallei] DUF1929 domain-containing protein [Burkholderia pseudomallei]. gi ... DUF1929 domain-containing protein [Burkholderia pseudomallei]. NCBI Reference Sequence: WP_004549088.1. Identical Proteins ...
... Taksaon Duangurai,1,2 Nitaya Indrawattana,1 and ... Burkholderia pseudomallei is a Gram-negative bacterium that causes melioidosis, which can be fatal in humans. Melioidosis is ... This review provides an overview of the survival and adaptation of B. pseudomallei to stressful conditions induced by hostile ... The adaptation of B. pseudomallei in host cells is also reviewed. The adaptive survival mechanisms of this pathogen mainly ...
... pseudomallei strain 08 and Burkholderia thailandensis, a non-pathogenic relative of B. pseudomallei, indicates that GIs are key ... Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei. Matthew T. G. Holden, Richard W. Titball, ... Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei. Matthew T. G. Holden, Richard W. Titball, ... Burkholderia pseudomallei is a recognized biothreat agent and the causative agent of melioidosis. This Gram-negative bacterium ...
Cross-Species Comparison of the Burkholderia pseudomallei, Burkholderia thailandensis, and Burkholderia mallei Quorum-Sensing ... Targeted Mutagenesis of Burkholderia thailandensis and Burkholderia pseudomallei through Natural Transformation of PCR ... pseudomallei strain 08 and Burkholderia thailandensis, a non-pathogenic relative of B. pseudomallei, indicates that GIs are key ... Genomic plasticity of the causative agent of melioidosis, Burkholderia pseudomallei. Matthew T. G. Holden, Richard W. Titball, ...
R. Balder, S. Lipski, J. J. Lazarus et al., "Identification of Burkholderia mallei and Burkholderia pseudomallei adhesins for ... Y. Song, C. Xie, Y. M. Ong, Y. H. Gan, and K. L. Chua, "The BpsIR quorum-sensing system of Burkholderia pseudomallei," Journal ... D. A. Rholl, L. A. Trunck, and H. P. Schweizer, "In vivo Himar1 transposon mutagenesis of Burkholderia pseudomallei," Applied ... A. Tuanyok, B. R. Leadem, R. K. Auerbach et al., "Genomic islands from five strains of Burkholderia pseudomallei," BMC Genomics ...
Burkholderia pseudomallei Description and significance. Burkholderia pseudomallei is a gram-negative bacterium with dimensions ... Burkholderia pseudomallei is a human and animal pathogen and is the cause of melioidosis, which is a disease native to ... Burkholderia pseudomallei is significant in its role of causing a disease known as melioidosis, which currently doesnt have a ... Burkholderia pseudomallei is thought to have biological warfare potential and its virulence is made evident by being listed as ...
Burkholderia Pseudomallei Genome Project. Burkholderia pseudomallei, the causative agent of the infectious disease melioidosis ... pseudomallei strains as well as between various bacteriophages harbored within B. pseudomallei. All data will be released ... pseudomallei strains from various geographic and clinical sources. Variable horizontal gene acquisition by B. pseudomallei is ... NIH-NIAID has funded this genome project to sequence nine phenotypically characterized strains of B. pseudomallei, as well as ...
Detection of Burkholderia pseudomallei in blood samples using polymerase chain reaction.. Rattanathongkom A1, Sermswan RW, ... A highly sensitive, specific, rapid and simple method to detect Burkholderia pseudomallei in blood samples was developed. Two ... pseudomallei. As little as 0.5 fg of B. pseudomallei DNA was detectable by this method. Experiments involving inoculation of ...
Burkholderia pseudomallei and Burkholderia mallei: Melioidosis and glanders. In: Mandell GL, Bennett JE, Raphael D, editors. ... Melioidosis is a bacterial infection caused by Burkholderia pseudomallei (formerly known as Pseudomonas pseudomallei or ... TABLE 2. Number of reported incidents of possible occupational exposure to Burkholderia pseudomallei involving research ... Fatal Burkholderia pseudomallei infection initially reported by Bacillus species, Ohio, 2013. Am J Trop Med Hyg 2014;91:743-6. ...
Burkholderia pseudomallei (Pseudomonas pseudomallei)Imported. Automatic assertion inferred from database entriesi ... tr,A0A069AXT4,A0A069AXT4_BURPE Formamidopyrimidine-DNA glycosylase OS=Burkholderia pseudomallei OX=28450 GN=mutM PE=3 SV=1 ... cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › ...
Å resolution crystal structure of a BpaA TAA head domain from Burkholderia pseudomallei, the pathogen that causes melioidosis. ... Methodology/Principal Findings Pathogenic species of Burkholderia contain an overabundance of TAAs, some of which have been ...
  • He investigates the role and function of pathogen-recognition-receptors and innate immunity in sepsis, with a special interest Burkholderia pseudomallei and Salmonella typhi infection and the role of diabetes as well as the gut microbiota during sepsis. (omicsonline.org)
  • Day 21 50% infectious dose values after intranasal and subcutaneous infection of BALB/c (A) and C57BL/6 (B) mice with bim Bm (n = 7) and bim Bp (n = 6) B. pseudomallei isolates. (cdc.gov)
  • The percentage of mice for a given bacterial strain for which evidence indicated establishment of B. pseudomallei infection (culture-positive growth from tissues) (C) and signs of neurologic involvement (e.g., head tilt, spinning behavior, and hind leg paresis) (D) was increased for animals exposed to bim Bm compared with bim Bp isolates. (cdc.gov)
  • The interactions of Burkholderia species and Acanthamoeba castellanii , a species of free-living amoeba, were studied to better understand the natural ecology of these organisms and to determine the effects amoeba interactions might have on pathogenesis. (byu.edu)
  • For non-clonal species such as Burkholderia pseudomallei, however, shared allelic states between distantly related isolates make it more difficult to identify population defining characteristics. (elsevier.com)
  • Virulence of bim Bm and bim Bp Burkholderia pseudomallei isolates. (cdc.gov)
  • Interestingly, it was found that after internalization by amoeba, B. pseudomallei have a significantly increased ability to both attach to, and grow within human monocytes, suggesting that such interactions increase the virulence capabilities of soil isolates. (byu.edu)
  • Moore, Emily Ann, "Interactions of Burkholderia pseudomallei and Acanthamoeba castellanii and Their Effects on Virulence in Human Monocytes" (2010). (byu.edu)
  • Overall, the B. pseudomallei strains showed evolutionary patterns similar to those of other chronic infections, including emergence of antibiotic resistance, genome reduction, and deleterious mutations in genes involved in virulence, metabolism, environmental survival, and cell wall components. (edu.au)
  • Here, we compared the genomes of B. pseudomallei isolates collected between similar to 4 and 55 months apart from seven chronically infected CF patients. (edu.au)
  • Here, we compared the genomes of B. pseudomallei isolates collected between ~4 and 55 months apart from seven chronically infected CF patients. (edu.au)
  • Population genetic dynamics of B. pseudomallei are particularly complicated relative to other bacterial pathogens, but the work here provides the ability for broad scale population assignment. (elsevier.com)
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