A species of BURKHOLDERIA considered to be an opportunistic human pathogen. It has been associated with various types of infections of nosocomial origin.
Infections with bacteria of the genus BURKHOLDERIA.
A group of phenotypically similar but genotypically distinct species (genomovars) in the genus BURKHOLDERIA. They are found in water, soil, and the rhizosphere of crop plants. They can act as opportunistic human pathogens and as plant growth promoting and biocontrol agents.
A genus of gram-negative, aerobic, rod-shaped bacteria. Organisms in this genus had originally been classified as members of the PSEUDOMONAS genus but overwhelming biochemical and chemical findings indicated the need to separate them from other Pseudomonas species, and hence, this new genus was created.
A species of gram-negative, aerobic bacteria that causes MELIOIDOSIS. It has been isolated from soil and water in tropical regions, particularly Southeast Asia.
An autosomal recessive genetic disease of the EXOCRINE GLANDS. It is caused by mutations in the gene encoding the CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR expressed in several organs including the LUNG, the PANCREAS, the BILIARY SYSTEM, and the SWEAT GLANDS. Cystic fibrosis is characterized by epithelial secretory dysfunction associated with ductal obstruction resulting in AIRWAY OBSTRUCTION; chronic RESPIRATORY INFECTIONS; PANCREATIC INSUFFICIENCY; maldigestion; salt depletion; and HEAT PROSTRATION.
A species of gram-negative bacteria that causes disease in plants. It is found commonly in the environment and is an opportunistic pathogen in humans.
Substances added to pharmaceutical preparations to protect them from chemical change or microbial action. They include ANTI-BACTERIAL AGENTS and antioxidants.
Methyl, propyl, butyl, and ethyl esters of p-hydroxybenzoic acid. They have been approved by the FDA as antimicrobial agents for foods and pharmaceuticals. (From Hawley's Condensed Chemical Dictionary, 11th ed, p872)
A plant genus of the family APIACEAE. The leaves are the source of cilantro and the seeds are the source of coriander, both of which are used in SPICES.
One of the Liliaceae used as a spice (SPICES) and traditional remedy. It contains alliin lyase and alliin, which is converted by alliin lyase to allicin, the pungent ingredient responsible for the aroma of fresh cut garlic.
A plant genus of the family LAMIACEAE best known for the thyme spice added to foods.
A species of gram-negative, aerobic bacteria that acts as both a human and plant pathogen.
The type species in the genus RALSTONIA. It is often found in the hospital ward as a contaminant of antiseptic and disinfectant solutions.
A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection.
A chloride channel that regulates secretion in many exocrine tissues. Abnormalities in the CFTR gene have been shown to cause cystic fibrosis. (Hum Genet 1994;93(4):364-8)

Lipopolysaccharide (LPS) from Burkholderia cepacia is more active than LPS from Pseudomonas aeruginosa and Stenotrophomonas maltophilia in stimulating tumor necrosis factor alpha from human monocytes. (1/432)

Whole cells and lipopolysaccharides (LPSs) extracted from Burkholderia cepacia, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Escherichia coli were compared in their ability to stimulate tumor necrosis factor alpha (TNF-alpha) from the human monocyte cell line MonoMac-6. B. cepacia LPS, on a weight-for-weight basis, was found to have TNF-alpha-inducing activity similar to that of LPS from E. coli, which was approximately four- and eightfold greater than the activity of LPSs from P. aeruginosa and S. maltophilia, respectively. The LPS-stimulated TNF-alpha production from monocytes was found to be CD14 dependent. These results suggest that B. cepacia LPS might play a role in the pathogenesis of inflammatory lung disease in cystic fibrosis, and in some patients it might be responsible, at least in part, for the sepsis-like cepacia syndrome.  (+info)

Comparison of isolation media for recovery of Burkholderia cepacia complex from respiratory secretions of patients with cystic fibrosis. (2/432)

Burkholderia cepacia selective agar (BCSA) has previously been devised for isolation of B. cepacia from respiratory secretions of patients with cystic fibrosis and tested under research laboratory conditions. Here we describe a study in which BCSA, oxidation-fermentation polymyxin bacitracin lactose agar (OFPBL), and Pseudomonas cepacia agar (PCA) were compared in routine culture procedures for the ability to grow B. cepacia and inhibit other organisms. Three hundred twenty-eight specimens from 209 patients at two pediatric centers and 328 specimens from 109 adults were tested. Plates were inoculated, incubated, and read for quality and quantity of growth at 24, 48, and 72 h. Five (1.5%) specimens from 4 (1.9%) children and 75 (22.9%) specimens from 16 (14.7%) adults grew B. cepacia complex. At 24, 48, and 72 h, BCSA achieved 43, 93, and 100% detection, respectively; OFPBL achieved 26, 84, and 96%, respectively; and PCA achieved 33, 74, and 84% detection, respectively. Quality was assessed as pinpoint or good growth. At 24 h, most cultures growing B. cepacia complex had pinpoint colonies. By 48 and 72 h, 48 and 69% of B. cepacia complex cultures, respectively, had good growth on BCSA, while on OFPBL 19 and 30%, respectively, had good growth and on PCA 11 and 18%, respectively, had good growth. BCSA was superior to OFPBL and PCA in suppressing organisms other than B. cepacia complex; 40 non-B. cepacia complex organisms were isolated from BCSA, 263 were isolated from OFPBL, and 116 were isolated from PCA. We conclude that BCSA is superior to OFPBL and PCA in its ability to support the growth of B. cepacia complex and to suppress other respiratory organisms.  (+info)

Structural elucidation of a novel exopolysaccharide produced by a mucoid clinical isolate of Burkholderia cepacia. Characterization of a trisubstituted glucuronic acid residue in a heptasaccharide repeating unit. (3/432)

The structure of the exopolysaccharide (EPS) produced by a clinical isolate of Burkholderia cepacia isolated from a patient with fibrocystic lung disease has been investigated. By means of methylation analyses, carboxyl reduction, partial depolymerization by fuming HCl and chemical degradations such as Smith degradation, lithiumethylenediamine degradation and beta-elimination, supported by GC/MS and NMR spectroscopic analyses, the repeat unit of the EPS has been identified and was shown to correspond to the acidic branched heptasaccharide with the following structure: [formula: see text]. This partially acetylated acidic polymer, distinguished by the presence of the less usual D-isomer of rhamnose and of a trisubstituted glucuronic acid residue, could represent the main EPS produced by this bacterial species.  (+info)

An epidemic of burkholderia cepacia transmitted between patients with and without cystic fibrosis. (4/432)

Burkholderia cepacia is an important pathogen in cystic fibrosis (CF) and an infrequent cause of nosocomial infection in non-CF patients. This report describes a large hospital outbreak that appeared to involve both patient groups, a previously unrecognized phenomenon. Ribotype restriction fragment length polymorphism (RFLP) profiles and pulsed-field gel electrophoresis-resolved macrochromosomal RFLPs were analyzed, a ribotype-based phylogenic tree was constructed, and case-control and cohort studies were performed. A single dominant clone was found in both CF and non-CF groups. Phylogenic analysis suggests that it has evolved independently and that such highly transmissible strains can emerge rapidly and randomly. Acquisition risk in the CF patients was linked to hospitalization (odds ratio=5.47, P=.0158, confidence interval=1. 28-26.86) and was associated with significantly increased mortality rates. Infection control policies must now consider this threat of transmission between non-CF and CF patients.  (+info)

Discrimination of Burkholderia multivorans and Burkholderia vietnamiensis from Burkholderia cepacia genomovars I, III, and IV by PCR. (5/432)

We present a PCR procedure for identification of Burkholderia cepacia, Burkholderia multivorans, and Burkholderia vietnamiensis. 16S and 23S ribosomal DNAs (rDNAs) of B. multivorans and B. vietnamiensis were sequenced and aligned with published sequences for definition of species-specific 18-mer oligonucleotide primers. Specific antisense 16S rDNA primers (for B. cepacia, 5'-AGC ACT CCC RCC TCT CAG-3'; for B. multivorans, 5'-AGC ACT CCC GAA TCT CTT-3') and 23S rDNA primers (for B. vietnamiensis, 5'-TCC TAC CAT GCG TGC AA-3') were paired with a general sense primer of 16S rDNAs (5'-AGR GTT YGA TYM TGG CTC AG-3') or with a sense primer of 23S rDNA (5'-CCT TTG GGT CAT CCT GGA-3'). PCR with these primers under optimized conditions is appropriate to specifically and rapidly identify B. multivorans, B. vietnamiensis, and B. cepacia (genomovars I, III, and IV are not discriminated). In comparison with the polyphasic taxonomic analyses presently necessary for species and genomovar identification within the B. cepacia complex, our procedure is more rapid and easier to perform and may contribute to clarifying the clinical significance of individual members of the complex in cystic fibrosis.  (+info)

Hydroxylation reaction catalyzed by the Burkholderia cepacia AC1100 bacterial strain. Involvement of the chlorophenol-4-monooxygenase. (6/432)

The Burkholderia cepacia AC1100 strain, known to degrade the herbicide, 2,4,5-Trichlorophenoxyacetic acid (2,4,5-T), is able to metabolize 4-hydroxyarylaldehyde, not only into the corresponding acid, but also into a new hydroquinone, 2,5-dihydroxyarylaldehyde. When incubated with resting AC1100 cells or cell-free extracts, syringaldehyde and 3,5-dimethoxy-4-hydroxybenzaldehyde were converted into such metabolites, identified by comparison of their mass and 1H-NMR spectra with those of authentic chemically synthesized samples. With 5-bromovanillin, only one metabolite was formed, the structure of which was identified as 2, 5-dihydroxy-4-methoxy-6-bromobenzaldehyde through 1H-NMR two-dimensional NOESY experiments. All these products result formally from a para hydroxylation of the phenol followed by the cis migration of the aldehyde. This reaction is the only one to be associated with the 2,4,5-T degradation pathway, as the acid formation was retained when the AC1100 strain had lost its degradation ability. Through competitive experiments with halophenols and methimazole, an alternative substrate of flavin monooxygenase, the chlorophenol-4-monooxygenase was recognized to be the enzyme involved in the hydroxylation of 4-hydroxyarylaldehyde. The purified enzyme, previously reported to catalyze the para hydroxylation or dehalogenating hydroxylation of chlorophenols, also promotes this hydroxylation reaction in the presence of NADH and FAD. The kcat value determined for the best substrate, syringaldehyde, 0. 08 s-1, was about 20% of that obtained for 2,6-dichlorophenol hydroxylation (0.38 s-1).  (+info)

Nitric oxide-induced potentiation of the killing of Burkholderia cepacia by reactive oxygen species: implications for cystic fibrosis. (7/432)

Burkholderia (formerly Pseudomonas) cepacia has emerged as an important pulmonary pathogen in cystic fibrosis, and survives within the lung despite a vigorous neutrophil-dominated immune response. Nitric oxide (NO) contributes to the antimicrobial activity of reactive oxygen species in the normal lung, but recent evidence suggests that inducible NO synthase is not expressed in the airway epithelial cells of cystic fibrosis (CF) patients. This may explain the failure of the neutrophil response to eliminate B. cepacia. To test this hypothesis, the present study examined the combined effect of NO, superoxide and H2O2 against B. cepacia. There was no killing of a highly transmissible strain by either superoxide or NO alone, but their combination reduced the bacterial count by >1000-fold over 75 min. This bactericidal activity was not sensitive to addition of superoxide dismutase, but was abrogated completely by catalase, suggesting that NO and hydrogen peroxide were the bactericidal mediators. Increased killing by NO in combination with H2O2 was seen for seven of a further 11 strains examined. The lack of NO in the lungs of CF patients may contribute to the survival of B. cepacia.  (+info)

Role of quinolinate phosphoribosyl transferase in degradation of phthalate by Burkholderia cepacia DBO1. (8/432)

Two distinct regions of DNA encode the enzymes needed for phthalate degradation by Burkholderia cepacia DBO1. A gene coding for an enzyme (quinolinate phosphoribosyl transferase) involved in the biosynthesis of NAD+ was identified between these two regions by sequence analysis and functional assays. Southern hybridization experiments indicate that DBO1 and other phthalate-degrading B. cepacia strains have two dissimilar genes for this enzyme, while non-phthalate-degrading B. cepacia strains have only a single gene. The sequenced gene was labeled ophE, due to the fact that it is specifically induced by phthalate as shown by lacZ gene fusions. Insertional knockout mutants lacking ophE grow noticeably slower on phthalate while exhibiting normal rates of growth on other substrates. The fact that elevated levels of quinolinate phosphoribosyl transferase enhance growth on phthalate stems from the structural similarities between phthalate and quinolinate: phthalate is a competitive inhibitor of this enzyme and the phthalate catabolic pathway cometabolizes quinolinate. The recruitment of this gene for growth on phthalate thus gives B. cepacia an advantage over other phthalate-degrading bacteria in the environment.  (+info)

The Burkholderia cepacia complex consists of nine closely related species: Burkholderia cepacia genomovars I and VI, Burkholderia multivorans (genomovar II), Burkholderia cenocepacia (genomovar III), Burkholderia stabilis (genomovar IV), Burkholderia vietnamiensis (genomovar V), Burkholderia ambifaria (genomovar VII), Burkholderia anthina (genomovar VIII), and Burkholderia pyrrocinia (genomovar IX) (2, 9, 10). Despite the advances that have been made in the understanding of the taxonomy and epidemiology of this group, infections of the respiratory tract with B. cepacia complex organisms still have a considerable impact on morbidity and mortality in cystic fibrosis (CF) patients (4, 5, 6). B. cepacia complex strains have also attracted attention as possible biocontrol and bioremediation agents that could be used as antagonists of soil-borne plant pathogens, as plant growth-promoting agents, and for the bioremediation of various recalcitrant xenobiotics (3, 8). To aid in identification, ...
Glowicz J, Crist M, Gould C, Moulton-Meissner H, Noble-Wang J, de Man TJB, Perry KA, Miller Z, Yang WC, Langille S, Ross J, Garcia B, Kim J, Epson E, Black S, Pacilli M, LiPuma JJ, Fagan R, B. cepacia Investigation Workgroup. 2018. A multistate investigation of health care-associated Burkholderia cepacia complex infections related to liquid docusate sodium contamination, January-October 2016. Am J Infect Control 46:649-655.. Brooks RB, Mitchell PK, Miller JR, Vasquez AM, Havlicek J, Lee H, Quinn M, Adams E, Baker D, Greeley R, Ross K, Daskalaki I, Walrath J, Moulton-Meissner H, Crist MB, Burkholderia cepacia Workgroup. 2018. Multistate Outbreak of Burkholderia cepacia Complex Bloodstream Infections After Exposure to Contaminated Saline Flush Syringes - United States, 2016-2017. Clin Infect Dis Off Publ Infect Dis Soc Am.. Becker SL, Berger FK, Feldner SK, Karliova I, Haber M, Mellmann A, Schäfers H-J, Gärtner B. 2018. Outbreak of Burkholderia cepacia complex infections associated with ...
At the beginning of this review it is essential to clarify the terminology that will be used to refer to the members of the Burkholderia cepacia complex and their relatives. The name B. cepacia will relate only to B. cepacia genomovar I. Strains resembling B. cepacia may belong to the B. cepacia complex, to otherBurkholderia species (for instance, Burkholderia gladioli), or to species from other genera (for instance,Ralstonia pickettii) that share some phenotypic or genotypic similarities with the B. cepacia complex. B. cepacia complex bacteria and organisms that may be confused with them will be altogether referred to as B. cepacia-like organisms. Most previous reports regarding these organisms were published before the recognition of the complicated taxonomic relationships between the different members of the B. cepacia complex; it is therefore unclear to what category the presumed B. cepacia isolates described would belong. For that reason, when such literature is cited, the name B. cepacia ...
The characteristic of most lipases is the interfacial activation at a lipid interface or in non-polar solvents. Interfacial activation is linked to a large conformational change of a lid, from a closed to an open conformation which makes the active site accessible for substrates. While for many lipases crystal structures of the closed and open conformation have been determined, the pathway of the conformational transition and possible bottlenecks are unknown. Therefore, molecular dynamics simulations of a closed homology model and an open crystal structure of Burkholderia cepacia lipase in water and toluene were performed to investigate the influence of solvents on structure, dynamics, and the conformational transition of the lid. The conformational transition of B. cepacia lipase was dependent on the solvent. In simulations of closed B. cepacia lipase in water no conformational transition was observed, while in three independent simulations of the closed lipase in toluene the lid gradually opened
Other names: ATCC 25416, B. cepacia, Burkholderia cepacia, Burkholderia cepacia genomovar I, CCUG 12691, CCUG 13226, CFBP 2227, CIP 80.24, DSM 7288, ICMP 5796, IFO 14074, JCM 5964, NBRC 14074, NCCB 76047, NCPPB 2993, NCTC 10743, NRRL B-14810, Pseudomonas cepacia, Pseudomonas kingii, Pseudomonas multivorans, strain 717-ICPB 25, strain Ballard 717 ...
Other names: ATCC 25416, B. cepacia, Burkholderia cepacia, Burkholderia cepacia genomovar I, CCUG 12691, CCUG 13226, CFBP 2227, CIP 80.24, DSM 7288, ICMP 5796, IFO 14074, JCM 5964, NBRC 14074, NCCB 76047, NCPPB 2993, NCTC 10743, NRRL B-14810, Pseudomonas cepacia, Pseudomonas kingii, Pseudomonas multivorans, strain 717-ICPB 25, strain Ballard 717 ...
1. Cystic Fibrosis Patient Registry, 2012 Annual Data Report Bethesda, MD: Cystic Fibrosis Foundation; 2013.. 2. Aaron SD, Ferris W, Henry DA, Speert DP, Macdonald NE. Multiple combination bactericidal antibiotic testing for patients with cystic fibrosis infected with Burkholderia cepacia. American journal of respiratory and critical care medicine 2000;161:1206-12. [PubMed]. 3. Aaron SD, Vandemheen KL, Ferris W, Fergusson D, Tullis E, Haase D, Berthiaume Y, Brown N, Wilcox P, Yozghatlian V, Bye P, Bell S, Chan F, Rose B, Jeanneret A, Stephenson A, Noseworthy M, Freitag A, Paterson N, Doucette S, Harbour C, Ruel M, MacDonald N. Combination antibiotic susceptibility testing to treat exacerbations of cystic fibrosis associated with multiresistant bacteria: a randomised, double-blind, controlled clinical trial. Lancet 2005;366:463-71. [PubMed]. 4. Avgeri SG, Matthaiou DK, Dimopoulos G, Grammatikos AP, Falagas ME. Therapeutic options for Burkholderia cepacia infections beyond co-trimoxazole: a ...
For a better understanding of the previously reported enantioselectivity of Pseudomonas cepacia lipase (PCL) in acylation of racemic primary alcohols, 2-methyl-3(or 4)-arylalkanols, molecular modeling of tetrahedral intermediates (TIs) at the active site was performed. The most probable conformers of TIs were elucidated and their interactions with the amino acid residues of the binding pockets at the enzyme active site were studied. The free energy difference between TIs of two enantiomers was approximated by the differences in potential energy and the solvent accesible surface area. Correlation between the HE(His286). . .O gamma (Ser87) hydrogen bond differences of diastereomeric, low energy gauche-TIs, and experimentally determined enantiomeric ratios was found. In agreement with the stereoelectronic theory, the gauche-TI precedes ester release.. ...
Burkholderia cepacia ATCC ® 17616D-5™ Designation: Genomic DNA from Burkholderia cepacia strain 249 TypeStrain=False Application:
Burkholderia cepacia ATCC ® 17616D-5™ Designation: Genomic DNA from Burkholderia cepacia strain 249 TypeStrain=False Application:
Quorum sensing is a mechanism for regulating proteobacterial gene expression in response to changes in cell population. In proteobacteria, N-acyl homoserine lactone (AHL) appears to be the most widely used signalling molecules in mediating, among others, the production of extracellular virulence factors for survival. In this work, the genome of B. cepacia strain GG4, a plasmid-free strain capable of AHL synthesis was explored. In silico analysis of the 6.6 Mb complete genome revealed the presence of a LuxI homologue which correspond to Type I quorum sensing. Here, we report the molecular cloning and characterization of this LuxI homologue, designated as BurI. This 609 bp gene was cloned and overexpressed in Escherichia coli BL21(DE3). The purified protein was approximately 25 kDa and is highly similar to several autoinducer proteins of the LuxI family among Burkholderia species. To verify the AHL synthesis activity of this protein, high resolution liquid chromatography-mass spectrometry analysis
The affected persons involved eight males and one female aged 46 to 69, who were admitted to Caritas Medical Centre from February 2017 to August 2018 respectively for management. Among them, seven were discharged, one remains hospitalized and one passed away in 2017.. The clinical diagnoses of the patients were infective spondylitis (4), sepsis (2), septic arthritis (2) and infective endocarditis (1).. Separately, the Burkholderia cepacia isolates from seven of the nine patients were found to be genetically identical upon laboratory testing.. Preliminary investigation revealed that all affected patients were people who injected drugs. Investigations are ongoing, a health department spokesman said.. Burkholderia cepacia is a bacteria which are commonly found in water, soil, and moist environments. It is generally not pathogenic to healthy person but those who have weakened immune system or chronic lung diseases may be more susceptible to infection with the bacteria.. The spokesman appealed that ...
Review question We looked for evidence of which antibiotics are best to treat a flare up of symptoms in people with cystic fibrosis with persistent Burkholderia cepacia complex lung infection.. Background Cystic fibrosis is a common inherited condition where the lungs often become blocked with mucus. This harms the lungs defences and often results in chronic, persistent infections that cannot be cleared by antibiotics. People with cystic fibrosis often need courses of antibiotics to reduce their symptoms (for instance cough, excess mucus and breathlessness) when these flare up or worsen. Such episodes are called exacerbations, and are usually treated with intravenous antibiotics (given through a drip into a vein). One group of bacteria that can infect the lungs of people with cystic fibrosis is called the Burkholderia cepacia complex. These closely-related bacteria are found widely in the environment and do not cause infections in healthy people who do not have cystic fibrosis. They are ...
TY - JOUR. T1 - Endemic Burkholderia cepacia bacteraemia. T2 - Clinical features and antimicrobial susceptibilities of isolates. AU - Yu, Wen Liang. AU - Wang, Der Yuan. AU - Lin, Cheng Wen. AU - Tsou, Mei Fen. PY - 1999. Y1 - 1999. N2 - Burkholderia cepacia has emerged as a nosocomial pathogen, causing numerous outbreaks, particularly among cystic fibrosis (CF) patients. Reports of clinical features of endemic B. cepacia bacteraemia in non-CF patients are rare. Twenty-five patients with B. cepacia bacteraemia were matched with 25 controls with nosocomial Escherichia coli bacteraemia at China Medical College Hospital, Taichung, Taiwan, over a period of 3 y. Case-patients included 16 men and 9 women, from 13 to 75 y. All had severe underlying diseases, most commonly malignancy (44%). Twenty-four patients (96%) had nosocomial infections. Five patients (20%) had polymicrobial bacteraemia. Our controls included 11 men and 14 women, age range 18-80 y. The most common underlying disease was malignancy ...
Review question. We reviewed the evidence for antibiotic treatment to cure early infection with Burkholderia cepacia complex in people with cystic fibrosis and prevent it becoming permanent.. Background. Cystic fibrosis is an inherited disease and people who have this disease produce large amounts of thick mucus which is difficult to clear. This mucus blocks up their lungs and digestive systems. People with cystic fibrosis suffer from lots of chest infections, which cause scarring of their airways. Eventually, they develop infections that cant be cured with antibiotics, so their lungs always contain lots of bugs, this is described as being chronically infected. One of these bugs, Burkholderia cepacia, causes a lot of problems for people with cystic fibrosis because it is very difficult to treat and makes their lung disease deteriorate faster than it otherwise would. This is an updated version of the review.. Search date. We last searched for any evidence on 12 March 2019.. Study ...
Burkholderia cepacia is recognized as an important pathogen in the lung infections of patients with cystic fibrosis. An inducible beta-lactamase activity has been associated with increased resistance to beta-lactam antibiotics in clinical isolates of B. cepacia. In this study, we report the revised sequence of the penA gene, which encodes the inducible penicillinase of B. cepacia, and show that it belongs to the molecular class A beta-lactamases and exhibits a high degree of similarity to the chromosomal beta-lactamase of Klebsiella oxytoca. Analysis of the nucleotide sequence of the DNA region directly upstream of the penA coding sequence revealed an open reading frame (penR), the transcription of which was oriented opposite to that of penA and whose initiation was 130 bp away from that of penA. Two potential ribosome-binding sites and two overlapping -10 and -35 promoter sequences were identified in the intercistronic region. The predicted translation product of penR was a polypeptide of 301 ...
The aim of this project was to investigate novel antimicrobial strategies against B. cepacia, based on natural antimicrobial compounds present in plants and human airways as protection against bacterial disease. The project focused on a panel of 20 strains of B. cepacia complex. Isolates included strains representing major epidemic clones of B. cepacia and exhibiting a range of susceptibilities to all classes of conventional antibiotics. The project is focused on two major themes. First, a study of antimicrobials from plants and second, examination of the antimicrobial activities of cationic peptides present in human airway secretions. The susceptibility of the B. cepacia strain panel to plant extracts was investigated, in particular the activity of aqueous garlic extract (AGE) and thyme oil. The MIC of thyme oil for all 20 strains was found to be 0.01%. MICs of AGE ranged from 0.25%-3%. Killing curves suggesting that AGE produces a slow killing effect over a twenty four hour period, whereas ...
TY - JOUR. T1 - Enzymatic resolution of (±)-cis-2-aminocyclopentanol and (±)-cis-2- aminocyclohexanol. AU - Luna, Amparo. AU - Astorga, Covadonga. AU - Fülöp, F.. AU - Gotor, Vicente. PY - 1998/12/24. Y1 - 1998/12/24. N2 - (±)-cis-N-Benzyloxycarbonyl-2-aminocyclopentanol was efficiently resolved by O-acylation with Pseudomonas cepacia lipase, as was (±)-cis-N- benzyloxycarbonyl-2-aminocyclohexanol when Candida antarctica lipase was used.. AB - (±)-cis-N-Benzyloxycarbonyl-2-aminocyclopentanol was efficiently resolved by O-acylation with Pseudomonas cepacia lipase, as was (±)-cis-N- benzyloxycarbonyl-2-aminocyclohexanol when Candida antarctica lipase was used.. UR - http://www.scopus.com/inward/record.url?scp=0032564662&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0032564662&partnerID=8YFLogxK. U2 - 10.1016/S0957-4166(98)00482-0. DO - 10.1016/S0957-4166(98)00482-0. M3 - Article. AN - SCOPUS:0032564662. VL - 9. SP - 4483. EP - 4487. JO - Tetrahedron Asymmetry. JF - ...
The frequency of isolation of Burkholderia cepacia from the sputum of cystic fibrosis (CF) patients is increasing. Using the human A549 lung epithelial cell line, we have investigated the ability of B. cepacia exoproducts to stimulate interleukin-8 (IL-8) release. Cell-free supernatants from a panel of CF clinical, non-CF clinical, and nonclinical B. cepacia isolates were found to stimulate IL-8 release, with levels ranging from 11.8 +/- 2.8 to 80.0 +/- 3.5 ng/ml. A similar pattern was seen at the level of the IL-8 mRNA. The bioactivity of the IL-8 was confirmed by examining its effect on the intracellular free calcium in neutrophils and inhibition by a neutralizing anti-IL-8 antibody. B. cepacia lipopolysaccharide, which was able to stimulate IL-8 release from monocytes, did not release IL-8 from the A549 cells. Furthermore, the stimulating ability of the bacterial cell-free supernatant was not diminished by polymyxin B, was markedly reduced by boiling, and appeared unrelated to ...
Nicholson, Audrey, Perry, John, James, Arthur, Stanforth, Stephen, Carnell, Sonya, Wilkinson, Kathryn, Khan, Anjam, de Soyza, Anthony and Gould, Kate (2012) In vitro activity of S-(3,4-dichlorobenzyl) isothiourea hydrochloride and novel structurally related compounds against multidrug-resistant bacteria, including Pseudomonas aeruginosa and Burkholderia cepacia complex. International Journal of Antimicrobial Agents, 39 (1). pp. 27-32. ISSN 0924-8579 de Soyza, Anthony, Meachery, Gerard, Hester, Katy, Nicholson, Audrey, Parry, Gareth, Tocewicz, Krzysztof, Pillay, Thasee, Clark, Stephen, Lordan, James, Schueler, Stephan, Fisher, Andrew, Dark, John, Gould, Kate and Corris, Paul (2010) Lung transplantation for patients with cystic fibrosis and Burkholderia cepacia complex infection: A single-center experience. The Journal of Heart and Lung Transplantation, 29 (12). pp. 1395-1404. ISSN 1053 2498 Meachery, Gerard, de Soyza, Anthony, Nicholson, Audrey, Parry, Gareth, Hasan, Asif, Tocewicz, Krzysztof, ...
Nurse Assist voluntarily recalled its I.V. Flush Syringes after becoming aware of patients that developed Burkholderia cepacia bloodstream infections while receiving intravenous care using prepackaged saline flushes from Nurse Assist. Nurse Assist is notifying its distributors and customers via email, phone call, and certified mail. Customers will receive instructions for returning product. For freight collect shipping information, please contact the Customer Service Team at 1-800-649-6800 ext. 10.. Nurse Assist is actively investigating the possible link to the infections with the Food and Drug Administration, the U.S. Centers for Disease Control, and various state health departments to ensure that the products are safe for their intended use. Until an investigation can be completed, Nurse Assist urges all healthcare facilities with affected product to discontinue use and return the product to the supplier.. Consumers with questions may contact the company via telephone at 1-800-649-6800 ext. ...
McNamara, Christopher J.; Leff, Laura Gunn (2002). In Situ Hybridization of Burkholderia Cepacia Cells. Humana Press 187-192. doi: 10.1385/1-59259-238-4:187. Retrieved from https://oaks.kent.edu/bscipubs/ ...
Free Online Library: Environmental Burkholderia cepacia complex isolates in human infections.(DISPATCHES) by Emerging Infectious Diseases; Health, general Infection Health aspects
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Over 6 days in May 2013, 4 BCs out of 14 in the special care baby unit of Base Hospital Avissawella, grew B. cepacia. BCs became positive after a mean of 3.5 days since admission. All four neonates developed high levels of inflammatory markers and thrombocytopaenia. Organism showed identical ABs in all isolates, with sensitivity to meropenem, ceftazidime, ciprofloxacin and resistance to trimethoprim and aminoglycosides. All babies were successfully treated with meropenem.. A 10% dextrose solution used for the neonates grew B. Cepacia on culture. This solution is not readily available in our setting and its prepared in the unit, using 5% and 50% dextrose combination. It was used for different babies at multiple times throughout the day by several nurses. Other samples that grew B. cepacia included a bottle of normal saline used as a multiple dose vial for preparation of intravenous (IV) drugs, a gallipot of sterile water used for suction of airway of a ventilated baby, 2 incubator humidifiers ...
Accepted name: 2-hydroxy-1,4-benzoquinone reductase. Reaction: 2-hydroxy-1,4-benzoquinone + NADH + H+ = hydroxyquinol + NAD+. For diagram of reaction click here.. Glossary: hydroxyquinol = 1,2,4-trihydroxybenzene. Other name(s): hydroxybenzoquinone reductase; 1,2,4-trihydroxybenzene:NAD oxidoreductase; NADH:2-hydroxy-1,4-benzoquinone oxidoreductase. Systematic name: NADH:2-hydroxy-1,4-benzoquinone oxidoreductase. Comments: A flavoprotein (FMN) that differs in substrate specificity from other quinone reductases. The enzyme in Burkholderia cepacia is inducible by 2,4,5-trichlorophenoxyacetate.. Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, UM-BBD, CAS registry number: 214466-94-1. References:. 1. Zaborina, O., Daubaras, D.L., Zago, A., Xun, L., Saido, K., Klem,T., Nikolic, D. and Chakrabarty, A.M. Novel pathway for conversion of chlorohydroxyquinol to maleylacetate in Burkholderia cepacia AC1100. J. Bacteriol. 180 (1998) 4667-4675. [PMID: 9721310]. ...
Antibiotic-resistant Burkholderia (Pseudomonas) cepacia is an important etiologic agent of nosocomial and cystic fibrosis infections. The primary resistance mechanism which has been reported is decreased outer membrane permeability. We previously reported the cloning and characterization of a chloramphenicol resistance determinant from an isolate of B. cepacia from a patient with cystic fibrosis that resulted in decreased drug accumulation. In the present studies we subcloned and sequenced the resistance determinant and identified gene products related to decreased drug accumulation. Additional drug resistances encoded by the determinant include resistances to trimethoprim and ciprofloxacin. Sequence analysis of a 3.4-kb subcloned fragment identified one complete and one partial open reading frame which are homologous with two of three components of a potential antibiotic efflux operon from Pseudomonas aeruginosa (mexA-mexB-oprM). On the basis of sequence data, outer membrane protein analysis, ...
General Information: Burkholderia cenocepacia MC0-3 was isolated from soil associated with maize roots. This species (genomovar III) is part of the Burkholderia cepacia complex, which consists of 9 closely related species, also known as genomovars. These organisms are commonly found in soil and all are opportunistic pathogens espcially in cystic fibrosis patients. ...
The Diels-Alder reaction is one of the most powerful synthetic tools in organic chemistry, and asymmetric Diels-Alder catalysis allows for rapid construction of chiral carbon scaffolds. For this reason, considerable effort has been invested in developing efficient and stereoselective organo- and biocatalysts. However, Diels-Alder is a virtually unknown reaction in Nature, and to engineer an enzyme into a Diels-Alderase is therefore a challenging task. Despite several successful designs of catalytic antibodies since the 1980s, their catalytic activities have remained low, and no true artificial Diels-Alderase enzyme was reported before 2010.. In this thesis, we employ state-of-the-art computational tools to study the mechanism of organocatalyzed Diels-Alder in detail, and to redesign existing enzymes into intermolecular Diels-Alder catalysts. Papers I-IV explore the mechanistic variations when employing increasingly activated reactants and the effect of catalysis. In particular, the ...
General Information: This organism (AU 1054) was isolated from the blood of a patient with CF. This microbe has three chromosomes. Opportunistic pathogen. This species (genomovar III) is part of the Burkholderia cepacia complex, which consists of 9 closely related species, also known as genomovars. These organisms are commonly found in soil and all are opportunistic pathogens espcially in cystic fibrosis patients. ...
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Virtually all patients with cystic fibrosis (CF) die of respiratory failure resulting from chronic progressive pulmonary infections. Pseudomonas aeruginosa and Pseudomonas (Burkholderia) cepacia are the two major bacterial pathogens responsible for the pulmonary deterioration in these patients. Tobramycin has variable inhibitory effects on these organisms, but in many isolates this inhibition is increased in vitro by exposure to the diuretic, amiloride. Aerosolized amiloride has been shown to be of clinical benefit in CF. The basis for this synergy is unknown. To examine the possibility that amiloride-tobramycin synergy is mediated through a bacterial efflux mechanism mediated by a multidrug resistant (MDR) protein, we studied the inhibitory effect of verapamil, a known MDR inhibitor on the tobramycin MIC in P. aeruginosa and P. cepacia using standard MIC and synergy testing. Verapamil had no effect on the tobramycin MIC in P. aeruginosa but was able to act synergistically with tobramycin in reducing
TY - CONF. T1 - A Contribution to Evaluate the Size of the Active Site of Lipases from Candida rugosa and Pseudomonas cepacia. AU - Gruber-Khadjawi, Mandana. AU - Hönig, Helmut. PY - 1993. Y1 - 1993. M3 - Poster. ER - ...
Can anybody tell me about the use of pseudomonas cepacia against nematodes and/or fungi. Does it work, are there already commercial products, how does it work ...
This work demonstrates the ring-cleavage pathways of catechol on Pseudomonas cepacia ATCC 29351, formed upon its growth on salicylate and benzoate, each as a sole carbon source. When grown on salicyla
In the past 2 decades, Burkholderia cepacia has emerged as a human pathogen causing numerous outbreaks, particularly among cystic fibrosis (CF) patients. One highly transmissible strain has spread across North America and Britain, and another between ...
4-methylphthalate catabolism in Burkholderia (Pseudomonas) cepacia Pc701: a gene encoding a phthalate-specific permease forms part of a novel gene cluster
Patients, Antibiotics, Bacteria, Biofilm, Biofilms, Cystic Fibrosis, Fibrosis, Sputum, Adults, Asthma, Death, Lung, Azithromycin, Burkholderia, Burkholderia Cepacia, Pseudomonas, Pseudomonas Aeruginosa, Rifampin, Treatment, Algorithm
Cystic fibrosis is an autosomal recessive disease affecting mostly Caucasian race. The disease is caused by mutations in CFTR gene. The most affected organ system is respiratory tract, because the changes lead to insufficient natural defence function of lungs, causing the lungs being susceptible to bacterial infections, which are the most common cause of death of CF patients. Among these bacteria is also Burkholderia cepacia, which causes rapid degradation of pulmonary tissue. Eradication of this bacterial infection is complicated by a number of antibiotic resistances and biofilm formation. Chicken antibodies against bacterial lectins are potential means of preventing bacterial lung infection of patients with CF. Using the affinity chromatography with BC2L-A immobilized column a specific antibody fraction was obtained. The immunoreactivity of the mixed antibodies, affinity purified antibodies and unbounded fraction was compared by ELISA. The comparison of immunoreactivity has emerged that the ...
Burkholderia cepacia complex bacteria are opportunistic pathogens that cause respiratory tract infections in susceptible patients, mainly people with cystic fibrosis. There is convincing evidence that B. cepacia complex bacteria can form biofilms, not only on abiotic surfaces (e.g., glass and plastics), but also on biotic surfaces such as epithelial cells, leading to the suggestion that biofilm formation plays a key role in persistent infection of cystic fibrosis lungs. This article presents an overview of the molecular mechanisms involved in B. cepacia complex biofilm formation, the increased resistance of sessile B. cepacia complex cells and the role of quorum sensing in B. cepacia complex biofilm formation.
Looking for online definition of Pseudomonas cepacia in the Medical Dictionary? Pseudomonas cepacia explanation free. What is Pseudomonas cepacia? Meaning of Pseudomonas cepacia medical term. What does Pseudomonas cepacia mean?
Bacteria of the Burkholderia cepacia complex (Bcc) are pathogens of humans, plants, and animals. Burkholderia cenocepacia is one of the most common Bcc species infecting cystic fibrosis (CF) patients and its carriage is associated with poor prognosis. In this study, we characterized a general O-link …
Regan KH, Bhatt J. Eradication therapy for Burkholderia cepacia complex in people with cystic fibrosis. Cochrane Database of Systematic Reviews 2016, Issue 11. Art. No.: CD009876. DOI: 10.1002/14651858.CD009876.pub3 ...
Pulmonary infection with Burkholderia cepacia complex (BCC) in patients with CF is often associated with a more rapid decline in lung function. Because of the resistance of BCC to many antibiotics, treatment options are often limited. New therapies to improve outcomes for patients infected with BCC are needed.. However, because of the unpredictable nature of this pulmonary infection in CF, patients with BCC infection have been excluded from many CF therapeutic trials.. Recent published trials in the United States, Australia, and the United Kingdom have all demonstrated clinical benefits from prolonged administration of azithromycin in CF. In these trials, the vast majority of patients were chronically infected with Pseudomonas aeruginosa.. Patients with BCC were excluded from the US and UK trials, and only four patients with BCC infection were enrolled in the Australian trial. Thus, the effectiveness of azithromycin in CF patients infected with BCC is largely unknown and deserves further ...
Burkholderia cepacia complex answers are found in the Johns Hopkins ABX Guide powered by Unbound Medicine. Available for iPhone, iPad, Android, and Web.
In this study, we compare our results for a G. mellonella infection model with previously published BCC data from other infection models, taking care to use many of the identical BCC strains previously tested in order to ensure valid comparison. We observed that G. mellonella, as an infection model for the BCC, possessed several advantages over other infection models. Using G. mellonella, we obtained similar results to both mouse and other alternative infection models with identical BCC strains. In our experience, no other alternative infection models as tested were as sensitive, accurate (with respect to mice and rat lung model results), or as robust or simple to use as the G. mellonella model. This was despite the fact G. mellonella possesses a relatively complex innate immune response, which is lacking in plant infection models. Furthermore, due to ethical and regulatory issues, it is now difficult to perform LD50 experiments with mice. In addition, amoebae and C. elegans are somewhat more ...
Appl Environ Microbiol 68: 173-180. Wang H, Marjomaki Book, Ovod Deal, Kulomaa MS( 2002) Numic purification of part iron in Sphingobium Palace ATCC 39723. Biochem Biophys Res Commun 299: 703-709. Nakamura haben, Motoyama file, Hirono S, Yamaguchi I( 2004) Identification, 4:00PM, and Fourth agriculture of inorganic book survey. Biochim Biophys Acta 1700: 151-159. Martin-Le Garrec G, Artaud I, Capeillere-Blandin C( 2001) war and ineffective pictures of the importance body from Burkholderia cepacia interpretation AC1100. Biochim Biophys Acta 1547: 288-301. Webb BN, Ballinger JW, Kim E, Belchik SM, Lam KS, Youn B, Nissen MS, Xun LY, Kang C( 2010) Characterization of Chlorophenol 4-Monooxygenase( TftD) and NADH: capitalism Oxidoreductase( TftC) of Burkholderia cepacia AC1100. Gisi MR, Xun LY( 2003) Characterization of chlorophenol 4-monooxygenase( TftD) and NADH: download Efectos del cambio climático en las costas de Chile 2009 deterioration physiology distress( TftC) of Burkholderia cepacia AC1100. ...
The structural gene (hdl IVa) for the Pseudomonas cepacia MBA4 2-haloacid halidohydrolase IVa (Hdl IVa) was isolated on a 1.6 kb fragment of Ps. cepacia MBA4 chromosomal DNA. The recombinant halidohydrolase was expressed in Escherichia coli and Pseudomonas putida and the structural gene was subcloned on to the tac expression vector pBTac1. High-level expression from the tac promoter was seen to be temperature-dependent, a consequence of the nucleotide sequence adjacent to the fragment encoding the halidohydrolase. The nucleotide sequence of the fragment encoding the Hdl IVa was determined and analysed. Three ATG codons were identified in one of the open reading frames and the one corresponding to the start of the hdl IVa structural gene was determined by comparison of the predicted amino acid sequences with the experimentally determined N-terminal sequences of halidohydrolase IVa. The hdl IVa gene encoded a 231-amino acid-residue protein of M(r) 25,900. The sequence and predicted structural data ...
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Polycyclic aromatic hydrocarbons (PAHs) are widespread in various ecosystems and are pollutants of great concern due to their potential toxicity, mutagenecity and carcinogenicity. Surfactant has become a hot topic for its wide application in the bioremediation of PAHs. The aim of this work is to explore a microcalorimetric method to determine the toxic effect of pyrene on Bacillus subtilis (B. subtilis) and the PAH-degrading bacteria Burkholderia cepacia (B. cepacia) and to evaluate the effect of Tween 80 on biodegradation of pyrene. Power-time curves were studied and calorimetric parameters including the growth rate constant (k), half inhibitory concentration (IC50), and total thermal effect (QT) were determined. B. subtilis, B. cepacia and B. cepacia with Tween 80 were completely inhibited when the concentration of pyrene were 200, 800 and 1600μgmL-1, respectively. B. cepacia shows better tolerance to pyrene than B. subtilis. Tween 80 significantly improves the biodegradation of pyrene by increasing
TY - JOUR. T1 - Pulmonary function, serum markers of inflammation, and IgG antibodies to core lipopolysaccharide of Bhrkholderia cepacia in adults with cystic fibrosis, following coloonization with Burkholderia cepacia. AU - Hendry, J.. AU - Nixon, L.. AU - Dodd, M.. AU - Elborn, Joseph. AU - Govan, J.. AU - Shale, D.J.. AU - Webb, A.K.. PY - 2000/1. Y1 - 2000/1. M3 - Article. VL - 29. SP - 8. EP - 10. JO - Pediatric Pulmonology. JF - Pediatric Pulmonology. SN - 8755-6863. ER - ...
Cystic fibrosis center coordinated care - How can cystic fibrosis affect p.I.E.S development? Certainly. Cystic Fibrosis (CF) most common life-limiting genetic disorder of Caucasians, affecting approximately 30K people in the US. A, impacting mainly respiratory function. In recent years patients symptoms better controlled by genetic findings and better medications. Emotional & phys. Development depend on severity, time in hospital, complications. Morb. & mort. Factors afect emotional, cognitive behavior.
Cook Childrens Cystic Fibrosis center is nationally accredited by the Cystic Fibrosis Foundation. Our pulmonologists work to provide care for children, adolescents and adults.
The Minnesota Cystic Fibrosis Center treats children, teens and adults with cystic fibrosis and other related lung and respiratory complications
TY - JOUR. T1 - The crystal structure of a triacylglycerol lipase from Pseudomonas cepacia reveals a highly open conformation in the absence of a bound inhibitor. AU - Kim, Kyeong Kyu. AU - Song, Hyun Kyu. AU - Shin, Dong Hae. AU - Hwang, Kwang Yeon. AU - Sun, Se Won. PY - 1997/2/15. Y1 - 1997/2/15. N2 - Background: Lipases, a family of enzymes which catalyze the hydrolysis of triglycerides, are widely distributed in many organisms. True lipases are distinguished from esterases by the characteristic interfacial activation they exhibit at an oil-water interface. Lipases are one of the most frequently used biocatalysts for organic reactions performed under mild conditions. Their biotechnological applications include food and oil processing and the preparation of chiral intermediates for the synthesis of enantiomerically pure pharmaceuticals. Recent structural studies on several lipases have provided some clues towards understanding the mechanisms of hydrolytic activity, interfacial activation, and ...
Increased treatment requirements of patients with cystic fibrosis who harbour a highly transmissible strain of Pseudomonas aeruginosa ...
The group of 17 opportunistic pathogens linked to severe infections in cystic fibrosis and other immunocompromised conditions has been known as the Burkholderia cepacia complex.[1] The inner Kdo residue of Burkholderia lipopolysaccharide (LPS), which is highly conserved across the genus, is flanked by 4-amino-4-deoxy-L-arabinose units (Ara4N).[2] Furthermore, the binding of Ara4N to the phosphate groups of lipid A has been purported as one of the reasons for hindered interaction with cationic agents and consequently, to induce antibiotic resistance.[3] We set out to synthesize a series of tenable Ara4N transferase (ArnT) inhibitors with the prospect of impeding the biosynthetic pathway towards activated Ara4N species.[4] By the same token, we intend to inhibit the transfer of Ara4N residues both to Burkholderia LPS core and lipid A in vitro. Our synthetic endeavours en route to C-glycosidic derivatives mimicking either the transition state of glycosyl transfer or ArnT natural substrate will be ...
Lineage: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Burkholderia; Burkholderia cepacia complex; Burkholderia ...
Newman LM, Wackett LP( 1995) book Englisch für Maschinenbauer and approach of war Methylene from Burkholderia cepacia G4. book Englisch für Maschinenbauer 1997 34:14066- 14076. Rehdorf J, Zimmer CL, Bornscheuer UT( 2009) Cloning, book Englisch, assimilation, and totalDownloads:2280 aldehyde of the dead oxidase from Pseudomonas putida JD1. Appl Environ Microbiol 75: 3106-3114. Appl Biochem Biotechnol 90: 187-197. Salituro FG, Demeter DA, Weintraub HJ, Lippert BJ, Resvick RJ, McDonald IA( 1994) book Englisch für und of cellular context. Haroune N, Combourieu B, Besse book, Sancelme M, Kloepfer A, et al. 2004) degradation of major by Rhodococcus anti-reverse. Appl Environ Microbiol 70: 6315-6319. Besse book Englisch für, Combourieu B, Boyse G, Sancelme M, De Wever H, et al. 2001) Long-range( 1)H-(15)N astrocyte capsaicin MolCare at other publication: a enoyl-acyl to provide music bread by two Rhodococcus cultures. Appl Environ Microbiol 67: 1412-1417. Negrete-Raymond AC, Weder B, Wackett LP( ...
The advantages of MALDI-TOF are that identification from pure culture can take minutes and is relatively cost-effective- other than the cost of the machine (approx. $200,000), relatively few reagents are required to process a sample. A recent paper investigating the use of MALDI-TOF to identify common CF pathogens, estimated that 71% of Pseudomonas aeruginosa isolates were identified by MALDI-TOF by day 2 of incubation, at which stage none had been identified by conventional culture2. Similarly, with Staphylococcus aureus, 89% were identified using MALDI-TOF by day 1 of incubation, whereas only 68% had been identified by culture on day 2. It can also identify bacteria which are slow growing much more quickly than by using standard culture techniques. In addition, it can also be used to identify yeasts and fungi, which are commonly found in CF patients. It can also determine differences between species of bacteria, such as those in the Burkholderia cepacia complex, which prove problematic for ...
Microorganisms living in extreme environments represent a huge reservoir of novel antimicrobial compounds and possibly of novel chemical families. Antarctica is one of the most extraordinary places on Earth and exhibits many distinctive features. Antarctic microorganisms are well known producers of valuable secondary metabolites. Specifically, several Antarctic strains have been reported to inhibit opportunistic human pathogens strains belonging to Burkholderia cepacia complex (Bcc). Herein, we applied a biodiscovery pipeline for the identification of anti-Bcc compounds. Antarctic sub-sea sediments were collected from the Ross Sea, and used to isolate 25 microorganisms, which were phylogenetically affiliated to three bacterial genera (Psychrobacter, Arthrobacter, and Pseudomonas) via sequencing and analysis of 16S rRNA genes. They were then subjected to a primary cell-based screening to determine their bioactivity against Bcc strains. Positive isolates were used to produce crude extracts from microbial
Burkholderia cepacia is a gram negative bacilli usually isolated from persons with genetic lung diseases such as Cystic fibrosis. It was discovered as a plant pathogen but later researchers have found it to be a notorious pathogen in humans. It is also a nosocomial pathogen which contaminates equipment and even disinfectants. In the other end of the spectrum, research suggests that these organisms maybe used as bio diesel and also as pesticides and fertilizers. This review of literature gives an idea of the organism and the gateway it opens for further research with a special note on the evolution of this genus as a nosocomial infection in Neonates.
We are always amazed and extremely grateful when people go out of their way to raise money for cystic fibrosis services. Some people are motivated by a family member or friend living with CF, and some people have heard about the disease in the press and offer to help.. Find Out More ...
In this first phase of the project we focused our efforts to express the B. cepacia lipase in E.coli using the T7 promoter to obtain a constitutive expression of the gene. Also, we plan to conduct some experiments using the protein OmpA in order to evaluate its effect on the secretion levels of our lipase.Evaluation of the level of expression of the gene (lipase) will be done by means of RT-PCR in real time which allows us to select the best parameters for its massive production.. To evaluate, qualitatively, the bioactivity of the lipase, solid media with egg yolk were prepared. We expect to observe a degradation ring around the colonies expressing T7+lipase and T7+OmpA+lipase and a smaller or null ring around non-transformed cells. This is important since it will tell us if there are problems with the secretion of our protein and thus, evaluate if there are folding problems, problems with the signal peptide, formation of inclusion bodies or others.. ...
Re preferred amongst saturated AHL molecules, whilst OCHSL and OCHSL are for carbonyl group.Similarly, for hydroxyl group OHCHSL and OHCHSL are favored.As opposed to bacteria, archaea have uncommon AHLs i.e.carboxylatedHSLs.Formation of biofilm is definitely the representative outcome of intricate Boldenone Cypionate supplier patterns of communication to enhance pathogenicity of bacteria.Within a classical instance, two bacterial species Pseudomonas aeruginosa and Burkholderia cepacia are known to reside with each other within a biofilm PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/21570335 in lungs of cystic fibrosis patients reflecting intraspecies, interspecies and interkingdom networking .Likewise, multispecies biofilms formed by several bacterial species involved in chronic wounds, dental plaque, and so on.exhibit complex networking among distinct organisms.This QS based groupbehavior of microbes is termed as `sociomicrobiology .SigMol can be a extensive resource of signaling molecules ...
The CF Center entrance is located in the Bernstein Pavilion between 15th and 16th Streets, facing Second Avenue. We are easily accessible by car and by public transportation.
FOSTER CITY, Calif., Feb 22, 2010 (BUSINESS WIRE) -- Gilead Sciences, Inc. (Nasdaq:GILD) today announced that the U.S. Food and Drug Administration (FDA) has granted marketing approval for Cayston(R)(aztreonam for inhalation solution) as a treatment to improve respiratory symptoms in cystic fibrosis (CF) patients with Pseudomonas aeruginosa (P. aeruginosa). Caystons safety and efficacy have not been established in pediatric patients below the age of 7, patients with forced expiratory volume in one second (FEV1) of less than 25 percent or greater than 75 percent predicted, or patients colonized with Burkholderia cepacia. Cayston is administered at a dose of 75 mg three times daily over a 28-day period and is delivered via the Altera(R) Nebulizer System, a portable, drug-specific delivery device using the eFlow(R) Technology Platform, developed by PARI Pharma GmbH. PARI Pharma also contributed to the development of Caystons drug formulation for delivery with the Altera Nebulizer System. Cayston ...
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Lipases from Burkholderia cepacia (Amano PS) and Candida antarctica B (CalB) were immobilized by sol-gel entrapment techniques, using a ternary silane precursors system with vinyl and phenyl non-hydrolyzable groups. The influence of temperature on the catalytic properties of native and immobilized lipases was investigated by incubating the biocatalysts for 24 h at temperatures between 40 C and 80 C, in n-hexane medium. A long term stability study was made with immobilized CaLB biocatalysts by incubation for 120 h in isooctane at 80 C. The acylation of aliphatic sec-alcohols by vinyl acetate was used as model reaction. Thermogravimetric analysis (TGA) was employed to evaluate the thermal stability and degradation of the immobilized biocatalysts ...
Lipases from Burkholderia cepacia (Amano PS) and Candida antarctica B (CalB) were immobilized by sol-gel entrapment techniques, using a ternary silane precursors system with vinyl and phenyl non-hydrolyzable groups. The influence of temperature on the catalytic properties of native and immobilized lipases was investigated by incubating the biocatalysts for 24 h at temperatures between 40 C and 80 C, in n-hexane medium. A long term stability study was made with immobilized CaLB biocatalysts by incubation for 120 h in isooctane at 80 C. The acylation of aliphatic sec-alcohols by vinyl acetate was used as model reaction. Thermogravimetric analysis (TGA) was employed to evaluate the thermal stability and degradation of the immobilized biocatalysts ...
Lipase from Burkholderia cepacia (PS) has been immobilized on Accurel MP 1000, and their performance has been compared to that of the most widely used immobilized commercial preparation of this enzyme. The maximum loading was 18 mg protein/g support, and its thermal and solvent stability was much higher than that of the commercial. PS preparations were used in hydrolysis of triacetin and methyl mandelate, in the esterification of oleic acid and ethanol and in the kinetic resolution of 1,3,4-tri-O-benzyl-myo-inositol (DL-1) using vinylacetate as activated acyl donor. For all reactions studied, PS on Accurel was more active than PS-IM. The conversion in the kinetic resolution of racemic DL-1 was optimized using response surface methodology. Optimal conditions were determined to be 2.0 mg/mL of substrate, temperature of 40oC, 2.0 ml of vinyl acetate and without water addition. Under these conditions, maximum loaded Accurel-PS preparation permitted to improve the activity in this kinetic resolution ...
Enter the following information to request a copy of the document from the responsible person.. Interaction of Klebsiella oxytoca and Burkholderia cepacia in dual-species batch cultures and biofilms as a function of growth rate and substrate concentration. ...
Nurse Assist Inc. announced a recall of 386,175 Normal Saline Flush IV Syringes due to possible Burkholderia Cepacia contamination.
Insight Pharmaceuticals, LLC, has recalled the nasal spray Nostrilla Nasal Decongestant due to potential contamination with Burkholderia cepacia, according to an FDA MedWatch safety alert.
To learn more about these bacteria, researchers sequenced the genomes of 24 strains collected from 2010 to 2014. All of the B. cepacia and B. contaminans strains were highly similar, and likely descended from original populations of these two bacteria that were present in the water dispenser when it left Earth.. The researchers conclude that the two bacterial species living within the dispenser are no more dangerous than similar strains that might be encountered on Earth. In the event of an infection, the bacteria can still be treated with common antibiotics.. The authors add: Within each species, the 19 B. cepacia and 5 B. contaminans recovered from the ISS were highly similar on a whole genome scale, suggesting each population may have stemmed from two distinct founding strains. The differences that can be observed among the isolates of the same species are primarily located within putative plasmids. We find that the populations of Burkholderia present in the ISS PWS are likely are not more ...
The SDSD is a database of the visual appearance of drug combinations reported in palliativedrugs.com prospective international multicentre surveys and also from individual reporting. There are currently over 2350 entries.This data is categorised as observational data, and a description of appeared compatible has been assigned if there was no obvious physical change in appearance of the combination in use, e.g. discoloration, clouding, or the precipitation of particles/crystals ...
C en 2002. Depuis 2008, le nombre de cas confirmés par le CNR sest stabilisé autour dune centaine de cas/an, soit 96 cas en 2017 et une incidence estimée à 0,85 cas confirmés/100.000 habitants. En 2017, le .... ...
Hello there everyone. I am writing to see if anyone has or had the same problems I have been having. I am 27 and have CF w/b.cepacia. Over this summer...
using System.Data.SqlClient; class Program { static void Main() { string connectionString = GetConnectionString(); // Open a sourceConnection to the AdventureWorks database. using (SqlConnection sourceConnection = new SqlConnection(connectionString)) { sourceConnection.Open(); // Perform an initial count on the destination table. SqlCommand commandRowCount = new SqlCommand( SELECT COUNT(*) FROM + dbo.BulkCopyDemoMatchingColumns;, sourceConnection); long countStart = System.Convert.ToInt32( commandRowCount.ExecuteScalar()); Console.WriteLine(Starting row count = {0}, countStart); // Get data from the source table as a SqlDataReader. SqlCommand commandSourceData = new SqlCommand( SELECT ProductID, Name, + ProductNumber + FROM Production.Product;, sourceConnection); SqlDataReader reader = commandSourceData.ExecuteReader(); // Open the destination connection. In the real world you would // not use SqlBulkCopy to move data from one table to the other // in the same database. This is ...
"Ornibactin production and transport properties in strains of Burkholderia vietnamiensis and Burkholderia cepacia (formerly ... Coenye T, Vandamme P, Govan JR, LiPuma JJ (October 2001). "Taxonomy and identification of the Burkholderia cepacia complex". ... LiPuma JJ (September 1998). "Burkholderia cepacia. Management issues and new insights". Clinics in Chest Medicine. 19 (3): 473- ... Speert DP (September 2002). "Advances in Burkholderia cepacia complex". Paediatric Respiratory Reviews. 3 (3): 230-5. doi: ...
Pseudomonas cepacia, a.k.a. Burkholderia cepacia. Nocardia. Fungi Aspergillus species. Aspergillus has a propensity to cause ... "Chronic granulomatous disease presenting in childhood with Pseudomonas cepacia septicaemia". The Journal of Infection. 27 (3): ...
Burkholderia cepacia:Friend or Foe? - Jennifer L. Parke Annual Review of Microbiology Annual Review of Phytopathology Some ... Bacterial strains from the Burkholderia cepacia complex (Bcc) are opportunistic pathogens in humans with cystic fibrosis and ... In 1950 he first described the bacteria, Pseudomonas cepacia, later Burkholderia spp., responsible for causing sour skin ...
"Burkholderia cepacia and cystic fibrosis". Archived from the original on 2008-09-16. Retrieved 2008-08-15.. ... Genomovar is a term commonly used within the genera Burkholderia and Agrobacterium to denote strains which are phylogenetically ...
Weinstein, Lenny; Knowlton, Christin A.; Smith, Miriam A. (2007-12-16). "Cervical osteomyelitis caused by Burkholderia cepacia ... The Burkholderia cepacia complex has been implicated in vertebral osteomyelitis in intravenous drug users. In general, ...
... with the type species Burkholderia cepacia (Palleroni and Holmes 1981) comb. Nov". Microbiology and Immunology. 36 (12): 1251- ... "Characterization of PCR-ribotyping for Burkholderia (Pseudomonas) cepacia". Journal of Clinical Microbiology. 32 (10): 2422- ... Yabuuchi, E.; Kosako, Y.; Yano, I.; Hotta, H.; Nishiuchi, Y. (1995). "Transfer of two Burkholderia and an Alcaligenes species ... Other strains previously classified in the genus Pseudomonas are now classified in the genera Burkholderia and Ralstonia. In ...
Burkholderia cepacia Proteus mirabilis and P. vulgaris Enterobacter spp. Bacteroides spp. Fusobacterium spp. Notable organisms ...
Burkholderia cepacia strain AIMST Ngse1 16S ribosomal RNA gene, partial sequence. NCBI. Burkholderia multivorans strain AIMST ... Burkholderiaceae Burkholderia Burkholderia cepacia strain AIMST Ngse1 (N. gracilis; Selangor, Malaysia; leaf tissue) ... Burkholderia multivorans strain AIMST Ngse2 (N. gracilis; Selangor, Malaysia; leaf tissue) Enterobacteriaceae Citrobacter ...
"Influence of Pseudomonas aeruginosa Exoproducts on Virulence Factor Production in Burkholderia cepacia: Evidence of ...
"Influence of Pseudomonas aeruginosa Exoproducts on Virulence Factor Production in Burkholderia cepacia: Evidence of ... P. aeruginosa exoproducts help B. cepacia to attach to different surfaces. The New Scientist: Lab chimp speaks his own language ...
Burkholderia cepacia • Campylobacter jejuni • Capnocytophaga ochracea • Corynebacterium xerosis • Enterobacter cloacae • ...
"A general protein O-glycosylation system within the Burkholderia cepacia complex is involved in motility and virulence". ... archaea and a number of pathogenic bacteria including Burkholderia cenocepacia, Neisseria gonorrhoeae and Acinetobacter ...
One particular method is the use of the bacterial complex Burkholderia cepacia, which is a registered control method. This ...
Burkholderia cepacia medium, and Burkholderia pseudomallei selective agar for clinical isolation of Burkholderia pseudomallei ... Ashdown's medium is a selective culture medium for the isolation and characterisation of Burkholderia pseudomallei (the ... 2007). "Biological relevance of colony morphology and phenotypic switching by Burkholderia pseudomallei". J Bacteriol. 189 (3 ...
The non-sterile form of Povidone iodine has a long history of intrinsic contamination with Burkholderia cepacia (aka ... "Biocide susceptibility of the Burkholderia cepacia complex". The Journal of Antimicrobial Chemotherapy. 63 (3): 502-10. doi: ... Pseudomonas cepacia), and other opportunistic pathogens. Its ability to harbor such microbes further underscores the importance ...
It is normally active against Moraxella catarrhalis, Brucella abortus, Burkholderia cepacia, Citrobacter species, Escherichia ...
Seven of the most common contaminants are: Aspergillus niger Burkholderia cepacia Clostridium difficile Escherichia coli ...
... a defunct industry standards organization Burkholderia cepacia complex, a bacterial pathogen Business Controls Corporation, ...
Burkholderia cepacia which causes bulb rot in onions as well as Xylophilus ampelinus which causes necrosis of grapevines. ...
... but resembles Burkholderia cepacia, which is also a nonfermenter and does not grow well on MacConkey agar. These two can be ... distinguished by the indole test or the Pyr test, both of which should be clearly negative for B. cepacia and positive for E. ...
Acinetobacter calcoaceticus Burkholderia cepacia Pseudomonas pseudoalcaligenes Peptostreptococcus spp Fusobacterium necrophorum ...
Species known to enter a VBNC state: E.M.S Aeromonas salmonicida Agrobacterium tumefaciens Burkholderia cepacia Burkholderia ...
... abortus Brucella melitensis Brucella suis Burkholderia Burkholderia mallei Burkholderia pseudomallei Burkholderia cepacia ...
... with the type species Burkholderia cepacia (Palleroni and Holmes 1981) comb. Nov". Microbiology and Immunology. 36 (12): 1251- ... cepacia, P. cocovenenans, P. delafieldii, P. facilis, P. flava, P. gladioli, P. glathei, P. glumae, P. graminis, P. huttiensis ... "Characterization of PCR-ribotyping for Burkholderia (Pseudomonas) cepacia". Journal of Clinical Microbiology. 32 (10): 2422- ... Yabuuchi, E.; Kosako, Y.; Yano, I.; Hotta, H.; Nishiuchi, Y. (1995). "Transfer of two Burkholderia and an Alcaligenes species ...
"Therefore, any patient of any age with a CGD type infection (Staphylococcus aureus, Burkholderia cepacia complex, Serratia ... "Any patient of any age with a CGD type infection (Staphylococcus aureus, Burkholderia cepacia complex, Serratia marcescens, ... "Any patient of any age with a CGD type infection (Staphylococcus aureus, Burkholderia cepacia complex, Serratia marcescens, ... "Any patient of any age with a CGD type infection (Staphylococcus aureus, Burkholderia cepacia complex, Serratia marcescens, ...
nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia ... "Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. ... B. arboris belongs to the Burkholderia cepacia complex. "J.P. Euzéby: List of bacterial names with standing in nomenclature". " ... Burkholderia arboris is a Gram-negative, aerobic, non-spore-forming bacterium of the genus Burkholderia and the family ...
"Burkholderia anthina sp. nov. and Burkholderia pyrrocinia, two additional Burkholderia cepacia complex bacteria, may confound ... "Polyphasic Characterisation of Burkholderia cepacia-Like Isolates Leading to the Emended Description of Burkholderia pyrrocinia ... "Burkholderia pyrrocinia". NCBI Taxonomy Browser. 60550. "Genus Burkholderia". List of Prokaryotic Names with Standing in ... Burkholderia pyrrocinia is a Gram-negative bacterium which has been found in soil as well as in the sputum of patients with ...
... is closely related to, and often mistaken for, a member of the Burkholderia cepacia complex. This ... PubMed article on PCR identification of Burkholderia EMedicine overview of Burkholderia Type strain of Burkholderia gladioli at ... Burkholderia gladioli can be distinguished from the other Burkholderia because it is oxidase negative B. gladioli is indole ... Burkholderia gladioli has been identified as a plant pathogen in onions, gladiolus, iris, and together with Burkholderia glumae ...
... bacterium from the genus of Burkholderia and the family of Burkholderiaceae and belongs to the Burkholderia cepacia complex, ... Rose, H.; Baldwin, A.; Dowson, C. G.; Mahenthiralingam, E. (2009). "Biocide susceptibility of the Burkholderia cepacia complex ... Burkholderia acidipaludis can cause biliary sepsis. "Genus Burkholderia". List of Prokaryotic Names with Standing in ... Type strain of Burkholderia contaminans at BacDive - the Bacterial Diversity Metadatabase v t e. ...
Burkholderia pseudomallei *Melioidosis. *Burkholderia mallei *Glanders. *Burkholderia cepacia complex. *Bordetella pertussis/ ...
Burkholderia pseudomallei *Melioidosis. *Burkholderia mallei *Glanders. *Burkholderia cepacia complex. *Bordetella pertussis/ ...
... და ზოგჯერ Burkholderia cepacia). 18 წლის ასაკისთვის პაციენტების 80% P. aeruginosa-ს სამიზნე ხდება, ხოლო 3.5%-ში B. cepacia-სი.[ ... Burkholderia) cepacia and multiresistant Pseudomonas aeruginosa". Neth J Med 46 (6): 280-87. . PMID 7643943. ... "Pseudomonas cepacia at summer camps for persons with cystic fibrosis". MMWR Morb. Mortal. Wkly. Rep. 42 (23): 456-9. June 1993 ... Pegues DA, Carson LA, Tablan OC, FitzSimmons SC, Roman SB, Miller JM, Jarvis WR (May 1994). "Acquisition of Pseudomonas cepacia ...
Burkholderia pseudomallei (Melioidosis) · Burkholderia mallei (Glanders) · Burkholderia cepacia complex · Bordetella pertussis/ ...
Burkholderia pseudomallei *Melioidosis. *Burkholderia mallei *Glanders. *Burkholderia cepacia complex. *Bordetella pertussis/ ...
Burkholderia cepacia medium, and Burkholderia pseudomallei selective agar for clinical isolation of Burkholderia pseudomallei ... 2011). "Rapid identification of Burkholderia pseudomallei and Burkholderia mallei by fluorescence in situ hybridization (FISH) ... Pathema Burkholderia resource "Burkholderia pseudomallei". NCBI Taxonomy Browser. 28450. ... or Burkholderia cepacia medium) may be used for selective isolation. Cultures typically become positive in 24 to 48 hours (this ...
Burkholderia pseudomallei *Melioidosis. *Burkholderia mallei *Glanders. *Burkholderia cepacia complex. *Bordetella pertussis/ ...
Burkholderia pseudomallei *Melioidosis. *Burkholderia mallei *Glanders. *Burkholderia cepacia complex. *Bordetella pertussis/ ...
Burkholderia pseudomallei *Melioidosis. *Burkholderia mallei *Glanders. *Burkholderia cepacia complex. *Bordetella pertussis/ ...
Burkholderia cepacia. *Chryseobacterium indologenes. *Edwardsiella. *Elizabethkingia meningoseptica. *Francisella tularensis ...
... some Burkholderia cepacia, Clostridium innocuum and Enterococcus faecium strains have developed resistance to ciprofloxacin to ...
... with the type species Burkholderia cepacia (Palleroni and Holmes 1981) comb. Nov". Microbiology and immunology 36 (12): 1251- ... cepacia, P. cocovenenans, P. delafieldii, P. facilis, P. flava, P. gladioli, P. glathei, P. glumae, P. graminis, P. huttiensis ... Yabuuchi, E.; Kosako, Y.; Yano, I.; Hotta, H.; Nishiuchi, Y. (1995). "Transfer of two Burkholderia and an Alcaligenes species ... outras cepas antes clasificadas no xénero Pseudomonas están agora clasificadas no xénero Burkholderia e Ralstonia.[12][13] ...
Burkholderia infection usually Burkholderia cepacia and other Burkholderia species Buruli ulcer Mycobacterium ulcerans ...
Burkholderia pseudomallei (Melioidosis) · Burkholderia mallei (Glanders) · Burkholderia cepacia complex · Bordetella pertussis/ ...
Burkholderia pseudomallei *Melioidosis. *Burkholderia mallei *Glanders. *Burkholderia cepacia complex. *Bordetella pertussis/ ...
Burkholderia pseudomallei *Melioidosis. *Burkholderia mallei *Glanders. *Burkholderia cepacia complex. *Bordetella pertussis/ ...
Burkholderia pseudomallei *Melioidosis. *Burkholderia mallei *Glanders. *Burkholderia cepacia complex. *Bordetella pertussis/ ...
Burkholderia pseudomallei *Melioidosis. *Burkholderia mallei *Glanders. *Burkholderia cepacia complex. *Bordetella pertussis/ ...
Burkholderia pseudomallei *Melioidosis. *Burkholderia mallei *Glanders. *Burkholderia cepacia complex. *Bordetella pertussis/ ...
Ionfhabhtú Burkholderia. usually Burkholderia cepacia and other Burkholderia species. Buruli ulcer. Mycobacterium ulcerans. ...
Burkholderia cepacia complex in the NCBI Taxonomy Browser Type strain of Burkholderia cepacia complex at BacDive, the Bacterial ... Burkholderia cepacia complex (BCC), or simply Burkholderia cepacia, is a group of catalase-producing, lactose-nonfermenting, ... The organism is usually cultured in Burkholderia cepacia agar (BC agar), which contains crystal violet and bile salts to ... Frost, F; Shaw, M; Nazareth, D (June 13, 2019). "Antibiotic therapy for chronic infection with Burkholderia cepacia complex in ...
General Information about B. cepacia. Burkholderia cepacia [burk-hōld-er-ee-uh si-pay-shee-uh] (also called B. cepacia) is the ... B cepacia is a known cause of infections in hospitalized patients.. Symptoms of B. cepacia infection. The effects of B. cepacia ... B. cepacia bacteria are often resistant to common antibiotics.. Populations susceptible to B. cepacia infection. B. cepacia ... Exposure to B. cepacia in the environment.. Treatment of B. cepacia infection. B. cepacia can be resistant to many common ...
The multifarious, multireplicon Burkholderia cepacia complex.. Mahenthiralingam E1, Urban TA, Goldberg JB. ... The Burkholderia cepacia complex (Bcc) is a collection of genetically distinct but phenotypically similar bacteria that are ... cepacia syndrome, which is characterized by a rapid decline in lung function that can include invasive disease. Here we ... Burkholderia cepacia complex/genetics*. *Burkholderia cepacia complex/pathogenicity*. *Burkholderia cepacia complex/physiology ...
Detection of Pseudomonas (Burkholderia) cepacia using PCR.. Campbell PW 3rd1, Phillips JA 3rd, Heidecker GJ, Krishnamani MR, ... We designed a PCR method to detect P. cepacia using P. cepacia 16 S rRNA sequences as the amplification target region. The PCR ... When sputum samples from three CF patients chronically colonized with P. cepacia and P. aeruginosa were analyzed, P. cepacia ... Pseudomonas cepacia colonization of the lung is associated with increased morbidity and mortality for cystic fibrosis (CF) ...
B cepacia,/i, genomovars, and consequently further segregation between genomovar III and non-genomovar III B cepacia infected ... Burkholderia cepacia,/i, complex infections in cystic fibrosis (CF) has recently been ascribed to differences in the virulence ... Cepacia-Like Syndrome Caused by Burkholderia mutivorans. George Zahariadis. ,1 Michelle H Levy. ,2 and Jane L Burns3. 1 ... Cepacia-Like Syndrome Caused by Burkholderia mutivorans,. Canadian Journal of Infectious Diseases and Medical Microbiology,. ...
Control of Cotton Seedling Damping-off in the Field by Burkholderia (Pseudomonas) cepacia. K. Zaki, I. J. Misaghi, and A. ... Related articles on Burkholderia cepacia from APS Journals. Mobilization, Cloning, and Sequence Determination of a Plasmid- ... Encoded Polygalacturonase from a Phytopathogenic Burkholderia (Pseudomonas) cepacia. Carlos F. Gonzalez, Elizabeth A. Pettit, ...
Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis and Burkholderia cepacia genomovars I and III. J ... Burkholderia cepacia: Friend or Foe?. Parke, J.L. 2000. Burkholderia cepacia: Friend or Foe? The Plant Health Instructor. DOI: ... Burkholderia cepacia genomovar VI, a new member of the Burkholderia cepacia complex isolated from cystic fibrosis patients. Int ... Proposal to accommodate Burkholderia cepacia genomovar VI as Burkholderia dolosa sp. nov. Int J Syst Evol Microbiol 54:689-691. ...
... we present a case of Burkholderia cepacia complex cervical osteomyelitis in an intravenous drug user. Burkholderia cepacia ... Burkholderia cepacia complex Cervical Osteomyelitis in an Intravenous Drug User. Sui Kwong Li1 and William B. Messer1,2 ... Burkholderia cepacia complex are closely related aerobic Gram-negative bacilli that are well described in infections among ... Burkholderia cepacia complex vertebral osteomyelitis is an infrequently described clinical syndrome in the literature though it ...
For additional information on B. cepacia please visit: Burkholderia cepacia in Healthcare Settings. ... FDA Alert August 8, 2017: FDA updates on 2017 Burkholderia cepacia contaminationexternal icon ... Multistate Outbreak of Burkholderia cepacia Infections Associated with Oral Liquid Docusate Sodium. ... Facilities that experience B. cepacia complex infections among non-cystic fibrosis patients or clusters of B. cepacia complex ...
... multireplicon Burkholderia cepacia complex." Nature. 3 Hua, C.N.C., Tokeshi, J. "Emergence of Burkholderia cepacia in Honolulu ... 6 "Burkholderia cepacia in Healthcare Settings." Centers for Disease Control and Prevention. 7 Gilchrist, F.J., Webb, A.K., ... This is often referred to as the Burkholderia cepacia complex (BCC) [1][2]. It was originally recognized by W. H. Burkholder in ... Burkholderia cepacia complex. From MicrobeWiki, the student-edited microbiology resource. Revision as of 21:03, 10 February ...
Genomic DNA from Burkholderia cepacia strain 249 TypeStrain=False Application: ... Genomic DNA from Burkholderia cepacia strain 249 [ATCC® 17616™] Biosafety Level 1 Biosafety classification is based on U.S. ... Burkholderia cepacia (Palleroni and Holmes) Yabuuchi et al. (ATCC® 17616D-5™) Strain Designations: Genomic DNA from ... Nucleotide (GenBank) : AY741333 Burkholderia cepacia strain ATCC 17616 16S ribosomal RNA gene, partial sequence ...
Burkholderia cepacia ATCC ® 25416™ Designation: UCB 717 TypeStrain=True Application: Assay of antimicrobial preservatives Media ... Burkholderia cepacia 16S ribosomal RNA gene, partial sequence. Nucleotide (GenBank) : AJ293344 Burkholderia cepacia partial ... Burkholderia cepacia (Palleroni and Holmes) Yabuuchi et al. (ATCC® 25416™) Strain Designations: UCB 717 [717-ICPB 25, Ballard ... Pyrimidine synthesis in Burkholderia cepacia ATCC 25416. Lett Appl Microbiol 21: 340-343, 1995. PubMed: 7576530 ...
... and biological pesticide Burkholderia cepacia because EPA canceled food registrations or deleted food uses from registrations ... 3. Burkholderia cepacia type Wisconsin. On August 28, 2002 (67 FR 55236) (FRL-7189-4), EPA published a notice in the Federal ... Allethrin, Bendiocarb, Burkholderia cepacia, Fenridazon potassium, and Molinate; Tolerance Actions. A Rule by the Environmental ... EPA is revoking the tolerance exemption in 40 CFR 180.1115 for residues of Burkholderia cepacia type Wisconsin in or on all raw ...
Human (hBD-1) and murine (mBD-1) b-defensins were examined for activity against B. cepacia. In contrast to the salt-sensitive ... The susceptibility of the B. cepacia strain panel to plant extracts was investigated, in particular the activity of aqueous ... The project focused on a panel of 20 strains of B. cepacia complex. Isolates included strains representing major epidemic ... In contrast, two hours incubation with AGE produced morphological changes in the cellular structure of B. cepacia consistent ...
Environmental Burkholderia cepacia complex isolates in human infections.(DISPATCHES) by Emerging Infectious Diseases; Health ... Identification and population structure of Burkholderia stabilis sp. nov. (formerly Burkholderia cepacia genomovar IV). J Clin ... cepacia+complex+isolates+in+human...-a0161011310. *APA style: Environmental Burkholderia cepacia complex isolates in human ... Commercial use of Burkholderia cepacia. Emerg Infect Dis. 1999;5:305-6. (9.) Govan JRW, Balendreau J, Vandamme P. Burkholderia ...
The Burkholderia cepacia complex is an important group of pathogens in patients with cystic fibrosis (CF). Although evidence ... Epidemiology of Burkholderia cepacia complex in patients with cystic fibrosis, Canada Emerg Infect Dis. 2002 Feb;8(2):181-7. ... The Burkholderia cepacia complex is an important group of pathogens in patients with cystic fibrosis (CF). Although evidence ... cepacia complex from genomovar III are the most potentially transmissible and that the B. cepacia epidemic strain marker is a ...
What is Burkholderia cepacia? Meaning of Burkholderia cepacia medical term. What does Burkholderia cepacia mean? ... Looking for online definition of Burkholderia cepacia in the Medical Dictionary? Burkholderia cepacia explanation free. ... Burkholderia cepacia. Burkholderia cepacia. a bacterial species found in rotted onions and in clinical specimens; commonly ... Synonym(s): Pseudomonas cepacia. Burkholderia cepacia. formerly Pseudomonas cepacia. A group of bacteria found in the ...
The Burkholderia cepaciacomplex (Bcc) is a group of 17 closely related species of the β-proteobacteria subdivision that emerged ... nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia ... Saldías MS, Valvano MA (2009) Interactions of Burkholderia cenocepacia and other Burkholderia cepacia complex bacteria with ... a complex within the Burkholderia cepacia complex comprises at least two novel species: Burkholderia contaminans sp. nov. and ...
Burkholderia mallei, Burkholderia pseudomallei,Burkholderia gladioli, Burkholderia plantarii,Burkholderia glumae, Burkholderia ... Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, and Burkholderia cepacia genomovars I and III. J. ... Burkholderia ubonensis, Burkholderia caledonica, Burkholderia fungorum,Burkholderia stabilis, and Burkholderia ambifaria(1, 10 ... 2001) Burkholderia cepacia genomovar VI, a new member of the Burkholderia cepacia complex isolated from cystic fibrosis ...
Pulmonary infection with Burkholderia cepacia complex (BCC) in patients with CF is often associated with a more rapid decline ... Azithromycin in Patients With CF, Infected With Burkholderia Cepacia Complex. The recruitment status of this study is unknown. ...
Burkholderia cepacia (Pseudomonas cepacia). 231. UniRef90_Q51645. Cluster: (S)-2-haloacid dehalogenase 4A. 3. ... Burkholderia cepacia (Pseudomonas cepacia). Paraburkholderia oxyphila. 231. UniRef50_Q51645. Cluster: (S)-2-haloacid ... Burkholderia cepacia (Pseudomonas cepacia). ,p>This subsection of the ,a href="http://www.uniprot.org/help/names_and_taxonomy_ ... sp,Q51645,HAD4_BURCE (S)-2-haloacid dehalogenase 4A OS=Burkholderia cepacia OX=292 GN=hdl IVa PE=1 SV=3 ...
B cepacia complex strains may remain endemic in CF treatment centers for many years. Responsible bacterial and host factors and ... Endemicity and inter-city spread of Burkholderia cepacia genomovar III in cystic fibrosis J Pediatr. 2001 Nov;139(5):643-9. doi ... Objectives: We sought to determine whether the same Burkholderia cepacia complex strain has persisted as the dominant clonal ... Conclusions: B cepacia complex strains may remain endemic in CF treatment centers for many years. Responsible bacterial and ...
... a colonização por Burkholderia cepacia complex (BCC), sobretudo... ... Colonização por Burkholderia cepacia complex em pacientes com doença pulmonar supurativa submetidos ao transplante pulmonar: ... Burkholderia Cepacia Complex colonization in patients with suppurative lung disease undergoing lung transplantation: impact on ... a colonização por Burkholderia cepacia complex (BCC), sobretudo o genomovar III, vem sendo relacionada a pior prognóstico e, ...
Burkholderia cepacia IBSD-LKBI exhibited the highest plant growth promotion and BCA. After 60 days of treatment, plant height, ... P Burkholderia cepacia (98%). Multiple sequence alignment & phylogram with closely related sequence accessions using MEGA ... Software revealed this naturally occurring biocontrol bacteria belongs to Burkholderia cepacia complex genomovar I based on the ... Burkholderia cepacia IBSD-LKBI exhibited the highest plant growth promotion and BCA. After 60 days of treatment, plant height, ...
Burkholderia cepacia (previously Pseudomonas cepacia) is an important pathogen in patients with cystic fibrosis (23). Twenty ... 1997) Occurrence of multiple genomovars of Burkholderia cepacia in cystic fibrosis patients and proposal of Burkholderia ... Quorum Sensing in Burkholderia cepacia: Identification of the LuxRI Homologs CepRI. Shawn Lewenza, Barbara Conway, E. P. ... Quorum Sensing in Burkholderia cepacia: Identification of the LuxRI Homologs CepRI. Shawn Lewenza, Barbara Conway, E. P. ...
The Burkholderia cepacia complex consists of nine closely related species: Burkholderia cepacia genomovars I and VI, ... Burkholderia anthina sp. nov. and Burkholderia pyrrocinia, two additional Burkholderia cepacia complex bacteria, may confound ... Updated Version of the Burkholderia cepacia Complex Experimental Strain Panel. Tom Coenye, Peter Vandamme, John J. LiPuma, John ... Updated Version of the Burkholderia cepacia Complex Experimental Strain Panel. Tom Coenye, Peter Vandamme, John J. LiPuma, John ...
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Burkholderia cepacia While colonisation with Burkholderia cepacia in cystic fibrosis (CF) may carry a poor prognosis, different ... Burkholderia cepacia colonisation in patients with cystic fibrosis: risk factors and clinical outcome. J Pediatr1995;107:382-7. ... Outcome of Burkholderia cepacia colonisation in children with cystic fibrosis following a hospital outbreak. Thorax1995;50:1194 ... Burkholderia (Pseudomonas) cepacia and cystic fibrosis: the epidemiology in Belgium. Acta Clin Belg1996;51:222-30. ...
An epidemic of burkholderia cepacia transmitted between patients with and without cystic fibrosis.. Authors. Holmes A, Nolan R ... Burkholderia cepacia is an important pathogen in cystic fibrosis (CF) and an infrequent cause of nosocomial infection in non-CF ...
Treatments to cure long-term infections with Burkholderia cepacia in people with cystic fibrosis. Review question ... This review highlights an urgent need for more research into new ways of treating long-term Burkholderia cepacia infection in ... Colonisation with Burkholderia cepacia complex species is linked to a more rapid decline in lung function and increases ... One of these bugs, Burkholderia cepacia, causes a lot of problems for people with cystic fibrosis because it is very difficult ...
  • The Pseudomonas vial (PD-109) with the supplement (S2-BCC-S) is specific for detecting the Burkholderia cepacia complex. (neogen.com)
  • The electrophoresis data of PCR products and comparison of band in samples from patients with standard strain ATCC 25416 Burkholderia cepacia and compare and analyse the PFGE size marker bands of Salmonella choleransuis serotype Braenderup H9812 strain, were the same. (ac.ir)
  • We describe the first reported case of a spontaneous septic arthritis caused by Burkholderia cepacia, the organism responsible for onion skin rot. (elsevier.com)
  • The comprehensive product portfolio covers the full workflow requirements for all non-sterile pharmaceutical B. cepacia product testing requirements: prepared culture media, dehydrated culture media and supplements, quality control organisms including B.cenocepacia ATCC BAA-245 and B.multivorans ATCC BAA-247, along with a full range of diagnostic products. (thermofisher.com)
  • In total, 79 % of patients were infected with Burkholderia cenocepacia (genomovar III), 18 % with Burkholderia multivorans (genomovar II) and less than 5 % of patients with B. cepacia (genomovar I), Burkholderia stabilis (genomovar IV) or Burkholderia vietnamiensis (genomovar V). The cblA and BCESM transmissibility markers were only detected in strains of B. cenocepacia. (cf.ac.uk)
  • Of the other eight species comprising the Bcc, only a few isolates belonging to B. cepacia genomovar I, Burkholderia stabilis, and Burkholderia pyrrocinia were found. (unige.it)
  • Studies of the prevalence of Burkholderia cepacia complex species amongst cystic fibrosis (CF) patients in different geographical regions, and the association between cross-infection and putative transmissibility markers, will further our understanding of these organisms and help to address infection-control issues. (cf.ac.uk)
  • Matthaiou DK , Chasou E , Atmatzidis S , Tsolkas P . A case of bacteremia due to Burkholderia cepacia in a patient without cystic fibrosis. (microbiologyresearch.org)
  • Chronic Burkholderia cepacia bronchiectasis in a non-cystic fibrosis individual. (microbiologyresearch.org)
  • In this study, the epidemiology of Burkholderia cepacia complex (Bcc) recovered from the sputum of 75 patients attending the Genoa Cystic Fibrosis (CF) Center at the Gaslini Children's Hospital (Genoa, Italy) was investigated, and the clinical course of the CF patients infected with the different species and genomovars of Bcc was evaluated. (unige.it)
  • Background: Complex of Burkholderia cepacia is one of the main and serious causes of infections in cystic fibrosis patients that can be highly transmissible. (ac.ir)
  • The aim of this research was to study genotyping of Burkholderia cepacia using PFGE method, and to evaluate diversity complex of clinical strains isolated from cystic fibrosis patients. (ac.ir)
  • Our report not only describes the case but also reviews the difficulty in treating B. cepacia infections. (elsevier.com)
  • The symptoms of B. cepacia infections vary widely from none at all to serious respiratory infections. (outbreaknewstoday.com)
  • Therapeutic options for Burkholderia cepacia infections beyond co-trimoxazole: a systematic review of the clinical evidence. (microbiologyresearch.org)
  • A recall of some lots of 2% chlorhexidine gluconate cloth patient preoperative skin preparation product because some tested positive for Burkholderia cepacia , which can cause respiratory infections in susceptible patients. (acphospitalist.org)
  • The FDA has issued a recall of certain docusate sodium solutions, used as stool softeners, due to a risk of product contamination with Burkholderia cepacia, resulting in serious, potentially life-threatening, infections. (farr.com)
  • Genetic diversity of isolate was performed by PFGE analysis according to Pulsenet guideline by using XbaI, SpeI as restriction enzyme which digests infrequently among the Burkholderia cepacia genome. (ac.ir)
  • In this study, B. cepacia complex isolates from CF patients in different regions of Europe were analysed. (cf.ac.uk)
  • In conclusion, B. cenocepacia was the greatest cause of cross-infection, and the most widely distributed B. cepacia complex species, within these CF populations. (cf.ac.uk)
  • However, cross-infection was not exclusive to B. cenocepacia and cblA and the BCESM were not absolute markers for transmissible B. cenocepacia, or other B. cepacia complex strains. (cf.ac.uk)
  • It is therefore suggested that CF centres cohort patients based on the presence or absence of B. cepacia complex infection and not on the basis of transmissibility marker-positive B. cenocepacia as previously suggested. (cf.ac.uk)
  • Update on the Burkholderia cepacia complex. (microbiologyresearch.org)
  • Simplify your lab workflow with non-sterile microbiological examination testing for B. cepacia complex. (thermofisher.com)
  • Many outbreaks of Burkholderia Cepacia Complex (BCC) infection are associated with contamination in personal care products (PCPS). (gentaur-online.com)
  • A strain of Burkholderia cepacia isolated by enrichment culture utilized L-2-amino-3-phosphonopropionic acid (phosphonoalanine) at concentrations up to 20 mM as a carbon, nitrogen, and phosphorus source in a phosphate-insensitive manner. (elsevier.com)
  • Despite post-operative resolution of this immunocompromised patient's shoulder symptoms, he was unable to survive the B. cepacia bacteremia. (elsevier.com)
  • Characteristics of patients with Burkholderia cepacia bacteremia. (microbiologyresearch.org)
  • This study investigates TCE degradation by willows (S. viminalis) and willows inoculated with three strains of B. cepacia (301C, PR1-31 and VM1330-pTOM), using chloride formation as an indicator of dehalogenation. (dtu.dk)
  • The three strains of B. cepacia did not enhance TCE mineralization. (dtu.dk)
  • Burkholderia cepacia AC1100 completely degrades 2,4,5-trichlorophenol, in which an FADH(2)-dependent monooxygenase (TftD) and an NADH:FAD oxidoreductase (TftC) catalyze the initial steps. (rcsb.org)
  • The U.S. Food and Drug Administration is advising consumers and health care professionals not to use any liquid drug or dietary supplement products manufactured by PharmaTech LLC of Davie, Florida, and labeled by Rugby Laboratories, Major Pharmaceuticals and Leader Brands , due to potential contamination with the bacteria Burkholderia cepacia (B. cepacia) and the risk for severe patient infection. (outbreaknewstoday.com)
  • Burkholderia cenocepacia is the predominant species recovered from the CF patients infected with Bcc at the Genoa CF Center. (unige.it)
  • The source of infection was most likely from hematogenous spread, as the patient's blood cultures were positive for B. cepacia. (elsevier.com)
  • Conclusion: Application of PFGE and identification of pulse-type is a potential tool to enhance the investigation of apparent nosocomial outbreaks of B.cepacia. (ac.ir)
  • According to the Centers for Disease Control and Prevention (CDC), B. cepacia poses the greatest threat to hospitalized patients, critically ill patients and people with health problems such as weakened immune systems and chronic lung diseases. (outbreaknewstoday.com)
  • Burkholderia cepacia is an aerobic, Gram-negative bacillus, which exhibits innate resistance to multiple antibiotics and disinfectants. (microbiologyresearch.org)
  • B. cepacia poses a serious threat to vulnerable patients, including infants and young children who still have developing immune systems," said FDA Commissioner Scott Gottlieb, M.D. "These products were distributed nationwide to retailers, health care facilities, pharmacies and sold online - making it important that parents, patients and health care providers be made aware of the potential risk and immediately stop using these products. (outbreaknewstoday.com)
  • Fingerprint Dive into the research topics of 'Spontaneous septic arthritis caused by Burkholderia cepacia. (elsevier.com)