Burkholderia cepacia: A species of BURKHOLDERIA considered to be an opportunistic human pathogen. It has been associated with various types of infections of nosocomial origin.Burkholderia Infections: Infections with bacteria of the genus BURKHOLDERIA.Burkholderia cepacia complex: A group of phenotypically similar but genotypically distinct species (genomovars) in the genus BURKHOLDERIA. They are found in water, soil, and the rhizosphere of crop plants. They can act as opportunistic human pathogens and as plant growth promoting and biocontrol agents.Burkholderia: A genus of gram-negative, aerobic, rod-shaped bacteria. Organisms in this genus had originally been classified as members of the PSEUDOMONAS genus but overwhelming biochemical and chemical findings indicated the need to separate them from other Pseudomonas species, and hence, this new genus was created.Burkholderia pseudomallei: A species of gram-negative, aerobic bacteria that causes MELIOIDOSIS. It has been isolated from soil and water in tropical regions, particularly Southeast Asia.Cystic Fibrosis: An autosomal recessive genetic disease of the EXOCRINE GLANDS. It is caused by mutations in the gene encoding the CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR expressed in several organs including the LUNG, the PANCREAS, the BILIARY SYSTEM, and the SWEAT GLANDS. Cystic fibrosis is characterized by epithelial secretory dysfunction associated with ductal obstruction resulting in AIRWAY OBSTRUCTION; chronic RESPIRATORY INFECTIONS; PANCREATIC INSUFFICIENCY; maldigestion; salt depletion; and HEAT PROSTRATION.Burkholderia cenocepacia: A species of gram-negative bacteria that causes disease in plants. It is found commonly in the environment and is an opportunistic pathogen in humans.Melioidosis: A disease of humans and animals that resembles GLANDERS. It is caused by BURKHOLDERIA PSEUDOMALLEI and may range from a dormant infection to a condition that causes multiple abscesses, pneumonia, and bacteremia.Burkholderia gladioli: A species of gram-negative, aerobic bacteria that acts as both a human and plant pathogen.2,4,5-Trichlorophenoxyacetic Acid: An herbicide with strong irritant properties. Use of this compound on rice fields, orchards, sugarcane, rangeland, and other noncrop sites was terminated by the EPA in 1985. (From Merck Index, 11th ed)Glanders: A contagious disease of horses that can be transmitted to humans. It is caused by BURKHOLDERIA MALLEI and characterized by ulceration of the respiratory mucosa and an eruption of nodules on the skin.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Bacterial Typing Techniques: Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping.Environmental Microbiology: The study of microorganisms living in a variety of environments (air, soil, water, etc.) and their pathogenic relationship to other organisms including man.Sputum: Material coughed up from the lungs and expectorated via the mouth. It contains MUCUS, cellular debris, and microorganisms. It may also contain blood or pus.Opportunistic Infections: An infection caused by an organism which becomes pathogenic under certain conditions, e.g., during immunosuppression.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Pseudomonas: A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants.Biodegradation, Environmental: Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.Biofilms: Encrustations, formed from microbes (bacteria, algae, fungi, plankton, or protozoa) embedding in extracellular polymers, that adhere to surfaces such as teeth (DENTAL DEPOSITS); PROSTHESES AND IMPLANTS; and catheters. Biofilms are prevented from forming by treating surfaces with DENTIFRICES; DISINFECTANTS; ANTI-INFECTIVE AGENTS; and antifouling agents.Rec A Recombinases: A family of recombinases initially identified in BACTERIA. They catalyze the ATP-driven exchange of DNA strands in GENETIC RECOMBINATION. The product of the reaction consists of a duplex and a displaced single-stranded loop, which has the shape of the letter D and is therefore called a D-loop structure.Tobramycin: An aminoglycoside, broad-spectrum antibiotic produced by Streptomyces tenebrarius. It is effective against gram-negative bacteria, especially the PSEUDOMONAS species. It is a 10% component of the antibiotic complex, NEBRAMYCIN, produced by the same species.RNA, Ribosomal, 16S: Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.Onions: Herbaceous biennial plants and their edible bulbs, belonging to the Liliaceae.Soil Microbiology: The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms.Random Amplified Polymorphic DNA Technique: Technique that utilizes low-stringency polymerase chain reaction (PCR) amplification with single primers of arbitrary sequence to generate strain-specific arrays of anonymous DNA fragments. RAPD technique may be used to determine taxonomic identity, assess kinship relationships, analyze mixed genome samples, and create specific probes.Bacterial Proteins: Proteins found in any species of bacterium.Virulence: The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.Anti-Bacterial Agents: Substances that reduce the growth or reproduction of BACTERIA.Polymorphism, Restriction Fragment Length: Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Genes, Bacterial: The functional hereditary units of BACTERIA.DNA, Ribosomal: DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.Electrophoresis, Gel, Pulsed-Field: Gel electrophoresis in which the direction of the electric field is changed periodically. This technique is similar to other electrophoretic methods normally used to separate double-stranded DNA molecules ranging in size up to tens of thousands of base-pairs. However, by alternating the electric field direction one is able to separate DNA molecules up to several million base-pairs in length.Pseudomonas aeruginosa: A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Gram-Negative Bacterial Infections: Infections caused by bacteria that show up as pink (negative) when treated by the gram-staining method.Quorum Sensing: A phenomenon where microorganisms communicate and coordinate their behavior by the accumulation of signaling molecules. A reaction occurs when a substance accumulates to a sufficient concentration. This is most commonly seen in bacteria.Chlorophenols: Phenols substituted with one or more chlorine atoms in any position.Trichloroethylene: A highly volatile inhalation anesthetic used mainly in short surgical procedures where light anesthesia with good analgesia is required. It is also used as an industrial solvent. Prolonged exposure to high concentrations of the vapor can lead to cardiotoxicity and neurological impairment.4-Butyrolactone: One of the FURANS with a carbonyl thereby forming a cyclic lactone. It is an endogenous compound made from gamma-aminobutyrate and is the precursor of gamma-hydroxybutyrate. It is also used as a pharmacological agent and solvent.Pseudomonas Infections: Infections with bacteria of the genus PSEUDOMONAS.Pyrrolnitrin: 3-Chloro-4-(3-chloro-2-nitrophenyl)pyrrole. Antifungal antibiotic isolated from Pseudomonas pyrrocinia. It is effective mainly against Trichophyton, Microsporium, Epidermophyton, and Penicillium.Microbial Sensitivity Tests: Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).Hydrogen Cyanide: Hydrogen cyanide (HCN); A toxic liquid or colorless gas. It is found in the smoke of various tobacco products and released by combustion of nitrogen-containing organic materials.Mimosa: A plant genus of the family FABACEAE that contains kukulkanin, a CHALCONE.Cross Infection: Any infection which a patient contracts in a health-care institution.Toluene: A widely used industrial solvent.Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Siderophores: Low-molecular-weight compounds produced by microorganisms that aid in the transport and sequestration of ferric iron. (The Encyclopedia of Molecular Biology, 1994)Stenotrophomonas maltophilia: A species of STENOTROPHOMONAS, formerly called Xanthomonas maltophilia, which reduces nitrate. It is a cause of hospital-acquired ocular and lung infections, especially in those patients with cystic fibrosis and those who are immunosuppressed.Granulomatous Disease, Chronic: A defect of leukocyte function in which phagocytic cells ingest but fail to digest bacteria, resulting in recurring bacterial infections with granuloma formation. When chronic granulomatous disease is caused by mutations in the CYBB gene, the condition is inherited in an X-linked recessive pattern. When chronic granulomatous disease is caused by CYBA, NCF1, NCF2, or NCF4 gene mutations, the condition is inherited in an autosomal recessive pattern.Species Specificity: The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.Bacteriological Techniques: Techniques used in studying bacteria.Benzethonium: Bactericidal cationic quaternary ammonium surfactant used as a topical anti-infective agent. It is an ingredient in medicaments, deodorants, mouthwashes, etc., and is used to disinfect apparatus, etc., in the food processing and pharmaceutical industries, in surgery, and also as a preservative. The compound is toxic orally as a result of neuromuscular blockade.Ceftazidime: Semisynthetic, broad-spectrum antibacterial derived from CEPHALORIDINE and used especially for Pseudomonas and other gram-negative infections in debilitated patients.Gene Expression Regulation, Bacterial: Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.Microbial Viability: Ability of a microbe to survive under given conditions. This can also be related to a colony's ability to replicate.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Methylomonas: A genus of gram-negative, aerobic, straight, curved, or branched rods which are motile by a single polar flagellum. (From Bergey's Manual of Determinative Bacteriology, 9th ed)

Lipopolysaccharide (LPS) from Burkholderia cepacia is more active than LPS from Pseudomonas aeruginosa and Stenotrophomonas maltophilia in stimulating tumor necrosis factor alpha from human monocytes. (1/432)

Whole cells and lipopolysaccharides (LPSs) extracted from Burkholderia cepacia, Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Escherichia coli were compared in their ability to stimulate tumor necrosis factor alpha (TNF-alpha) from the human monocyte cell line MonoMac-6. B. cepacia LPS, on a weight-for-weight basis, was found to have TNF-alpha-inducing activity similar to that of LPS from E. coli, which was approximately four- and eightfold greater than the activity of LPSs from P. aeruginosa and S. maltophilia, respectively. The LPS-stimulated TNF-alpha production from monocytes was found to be CD14 dependent. These results suggest that B. cepacia LPS might play a role in the pathogenesis of inflammatory lung disease in cystic fibrosis, and in some patients it might be responsible, at least in part, for the sepsis-like cepacia syndrome.  (+info)

Comparison of isolation media for recovery of Burkholderia cepacia complex from respiratory secretions of patients with cystic fibrosis. (2/432)

Burkholderia cepacia selective agar (BCSA) has previously been devised for isolation of B. cepacia from respiratory secretions of patients with cystic fibrosis and tested under research laboratory conditions. Here we describe a study in which BCSA, oxidation-fermentation polymyxin bacitracin lactose agar (OFPBL), and Pseudomonas cepacia agar (PCA) were compared in routine culture procedures for the ability to grow B. cepacia and inhibit other organisms. Three hundred twenty-eight specimens from 209 patients at two pediatric centers and 328 specimens from 109 adults were tested. Plates were inoculated, incubated, and read for quality and quantity of growth at 24, 48, and 72 h. Five (1.5%) specimens from 4 (1.9%) children and 75 (22.9%) specimens from 16 (14.7%) adults grew B. cepacia complex. At 24, 48, and 72 h, BCSA achieved 43, 93, and 100% detection, respectively; OFPBL achieved 26, 84, and 96%, respectively; and PCA achieved 33, 74, and 84% detection, respectively. Quality was assessed as pinpoint or good growth. At 24 h, most cultures growing B. cepacia complex had pinpoint colonies. By 48 and 72 h, 48 and 69% of B. cepacia complex cultures, respectively, had good growth on BCSA, while on OFPBL 19 and 30%, respectively, had good growth and on PCA 11 and 18%, respectively, had good growth. BCSA was superior to OFPBL and PCA in suppressing organisms other than B. cepacia complex; 40 non-B. cepacia complex organisms were isolated from BCSA, 263 were isolated from OFPBL, and 116 were isolated from PCA. We conclude that BCSA is superior to OFPBL and PCA in its ability to support the growth of B. cepacia complex and to suppress other respiratory organisms.  (+info)

Structural elucidation of a novel exopolysaccharide produced by a mucoid clinical isolate of Burkholderia cepacia. Characterization of a trisubstituted glucuronic acid residue in a heptasaccharide repeating unit. (3/432)

The structure of the exopolysaccharide (EPS) produced by a clinical isolate of Burkholderia cepacia isolated from a patient with fibrocystic lung disease has been investigated. By means of methylation analyses, carboxyl reduction, partial depolymerization by fuming HCl and chemical degradations such as Smith degradation, lithiumethylenediamine degradation and beta-elimination, supported by GC/MS and NMR spectroscopic analyses, the repeat unit of the EPS has been identified and was shown to correspond to the acidic branched heptasaccharide with the following structure: [formula: see text]. This partially acetylated acidic polymer, distinguished by the presence of the less usual D-isomer of rhamnose and of a trisubstituted glucuronic acid residue, could represent the main EPS produced by this bacterial species.  (+info)

An epidemic of burkholderia cepacia transmitted between patients with and without cystic fibrosis. (4/432)

Burkholderia cepacia is an important pathogen in cystic fibrosis (CF) and an infrequent cause of nosocomial infection in non-CF patients. This report describes a large hospital outbreak that appeared to involve both patient groups, a previously unrecognized phenomenon. Ribotype restriction fragment length polymorphism (RFLP) profiles and pulsed-field gel electrophoresis-resolved macrochromosomal RFLPs were analyzed, a ribotype-based phylogenic tree was constructed, and case-control and cohort studies were performed. A single dominant clone was found in both CF and non-CF groups. Phylogenic analysis suggests that it has evolved independently and that such highly transmissible strains can emerge rapidly and randomly. Acquisition risk in the CF patients was linked to hospitalization (odds ratio=5.47, P=.0158, confidence interval=1. 28-26.86) and was associated with significantly increased mortality rates. Infection control policies must now consider this threat of transmission between non-CF and CF patients.  (+info)

Discrimination of Burkholderia multivorans and Burkholderia vietnamiensis from Burkholderia cepacia genomovars I, III, and IV by PCR. (5/432)

We present a PCR procedure for identification of Burkholderia cepacia, Burkholderia multivorans, and Burkholderia vietnamiensis. 16S and 23S ribosomal DNAs (rDNAs) of B. multivorans and B. vietnamiensis were sequenced and aligned with published sequences for definition of species-specific 18-mer oligonucleotide primers. Specific antisense 16S rDNA primers (for B. cepacia, 5'-AGC ACT CCC RCC TCT CAG-3'; for B. multivorans, 5'-AGC ACT CCC GAA TCT CTT-3') and 23S rDNA primers (for B. vietnamiensis, 5'-TCC TAC CAT GCG TGC AA-3') were paired with a general sense primer of 16S rDNAs (5'-AGR GTT YGA TYM TGG CTC AG-3') or with a sense primer of 23S rDNA (5'-CCT TTG GGT CAT CCT GGA-3'). PCR with these primers under optimized conditions is appropriate to specifically and rapidly identify B. multivorans, B. vietnamiensis, and B. cepacia (genomovars I, III, and IV are not discriminated). In comparison with the polyphasic taxonomic analyses presently necessary for species and genomovar identification within the B. cepacia complex, our procedure is more rapid and easier to perform and may contribute to clarifying the clinical significance of individual members of the complex in cystic fibrosis.  (+info)

Hydroxylation reaction catalyzed by the Burkholderia cepacia AC1100 bacterial strain. Involvement of the chlorophenol-4-monooxygenase. (6/432)

The Burkholderia cepacia AC1100 strain, known to degrade the herbicide, 2,4,5-Trichlorophenoxyacetic acid (2,4,5-T), is able to metabolize 4-hydroxyarylaldehyde, not only into the corresponding acid, but also into a new hydroquinone, 2,5-dihydroxyarylaldehyde. When incubated with resting AC1100 cells or cell-free extracts, syringaldehyde and 3,5-dimethoxy-4-hydroxybenzaldehyde were converted into such metabolites, identified by comparison of their mass and 1H-NMR spectra with those of authentic chemically synthesized samples. With 5-bromovanillin, only one metabolite was formed, the structure of which was identified as 2, 5-dihydroxy-4-methoxy-6-bromobenzaldehyde through 1H-NMR two-dimensional NOESY experiments. All these products result formally from a para hydroxylation of the phenol followed by the cis migration of the aldehyde. This reaction is the only one to be associated with the 2,4,5-T degradation pathway, as the acid formation was retained when the AC1100 strain had lost its degradation ability. Through competitive experiments with halophenols and methimazole, an alternative substrate of flavin monooxygenase, the chlorophenol-4-monooxygenase was recognized to be the enzyme involved in the hydroxylation of 4-hydroxyarylaldehyde. The purified enzyme, previously reported to catalyze the para hydroxylation or dehalogenating hydroxylation of chlorophenols, also promotes this hydroxylation reaction in the presence of NADH and FAD. The kcat value determined for the best substrate, syringaldehyde, 0. 08 s-1, was about 20% of that obtained for 2,6-dichlorophenol hydroxylation (0.38 s-1).  (+info)

Nitric oxide-induced potentiation of the killing of Burkholderia cepacia by reactive oxygen species: implications for cystic fibrosis. (7/432)

Burkholderia (formerly Pseudomonas) cepacia has emerged as an important pulmonary pathogen in cystic fibrosis, and survives within the lung despite a vigorous neutrophil-dominated immune response. Nitric oxide (NO) contributes to the antimicrobial activity of reactive oxygen species in the normal lung, but recent evidence suggests that inducible NO synthase is not expressed in the airway epithelial cells of cystic fibrosis (CF) patients. This may explain the failure of the neutrophil response to eliminate B. cepacia. To test this hypothesis, the present study examined the combined effect of NO, superoxide and H2O2 against B. cepacia. There was no killing of a highly transmissible strain by either superoxide or NO alone, but their combination reduced the bacterial count by >1000-fold over 75 min. This bactericidal activity was not sensitive to addition of superoxide dismutase, but was abrogated completely by catalase, suggesting that NO and hydrogen peroxide were the bactericidal mediators. Increased killing by NO in combination with H2O2 was seen for seven of a further 11 strains examined. The lack of NO in the lungs of CF patients may contribute to the survival of B. cepacia.  (+info)

Role of quinolinate phosphoribosyl transferase in degradation of phthalate by Burkholderia cepacia DBO1. (8/432)

Two distinct regions of DNA encode the enzymes needed for phthalate degradation by Burkholderia cepacia DBO1. A gene coding for an enzyme (quinolinate phosphoribosyl transferase) involved in the biosynthesis of NAD+ was identified between these two regions by sequence analysis and functional assays. Southern hybridization experiments indicate that DBO1 and other phthalate-degrading B. cepacia strains have two dissimilar genes for this enzyme, while non-phthalate-degrading B. cepacia strains have only a single gene. The sequenced gene was labeled ophE, due to the fact that it is specifically induced by phthalate as shown by lacZ gene fusions. Insertional knockout mutants lacking ophE grow noticeably slower on phthalate while exhibiting normal rates of growth on other substrates. The fact that elevated levels of quinolinate phosphoribosyl transferase enhance growth on phthalate stems from the structural similarities between phthalate and quinolinate: phthalate is a competitive inhibitor of this enzyme and the phthalate catabolic pathway cometabolizes quinolinate. The recruitment of this gene for growth on phthalate thus gives B. cepacia an advantage over other phthalate-degrading bacteria in the environment.  (+info)

*Burkholderia cepacia complex

... (BCC), or simply Burkholderia cepacia, is a group of catalase-producing, lactose-nonfermenting, ... "Burkholderia cepacia complex". NCBI Taxonomy Browser. 87882. Type strain of Burkholderia cepacia complex at BacDive - the ... UMR: Burkholderia cepacia CFF: Research Pathema-Burkholderia Resource Loutet SA, Valvano MA (October 2010). "A decade of ... The organism is usually cultured in Burkholderia cepacia agar (BC agar) which contains crystal violet and bile salts to inhibit ...

*Hypothiocyanite

Burkholderia cepacia • Campylobacter jejuni • Capnocytophaga ochracea • Corynebacterium xerosis • Enterobacter cloacae • ...

*Chronic granulomatous disease

Pseudomonas cepacia, a.k.a. Burkholderia cepacia. Nocardia. fungi Aspergillus species. Aspergillus has a propensity to cause ... "Chronic granulomatous disease presenting in childhood with Pseudomonas cepacia septicaemia". J. Infect. 27 (3): 301-4. doi: ...

*Genomovar

Burkholderia cepacia and cystic fibrosis. ... is a term commonly used within the genera Burkholderia and ...

*Burkholderia cenocepacia

... is Burkholderia cenocepacia, a member of a bacterial group collectively referred to as the Burkholderia cepacia complex (Bcc ... Lipuma J (2005). "Update on the Burkholderia cepacia complex". Curr Opin Pulm Med. 11 (6): 528-33. doi:10.1097/01.mcp. ... Mahenthiralingam E, Vandamme P (2005). "Taxonomy and pathogenesis of the Burkholderia cepacia complex". Chron Respir Dis. 2 (4 ... "Burkholderia cenocepacia". NCBI Taxonomy Browser. 95486. Type strain of Burkholderia cenocepacia at BacDive - the Bacterial ...

*Burkholderia pseudomultivorans

... belongs to the Burkholderia cepacia complex. LSPN bacterio.net Straininfo of Burkholderia ... Burkholderia pseudomultivorans is a bacterium from the genus Burkholderia and the family Burkholderiaceae which was isolated ... pseudomultivorans Srinivasan, S; Kim, J; Kang, S. R.; Jheong, W. H.; Lee, S. S. (2013). "Burkholderia humi sp. nov., isolated ...

*Cystic fibrosis

Eventually, Pseudomonas aeruginosa (and sometimes Burkholderia cepacia) dominates. By 18 years of age, 80% of patients with ... Burkholderia) cepacia and multiresistant Pseudomonas aeruginosa". Neth J Med. 46 (6): 280-87. doi:10.1016/0300-2977(95)00020-N ... "Pseudomonas cepacia at summer camps for persons with cystic fibrosis". MMWR Morb. Mortal. Wkly. Rep. 42 (23): 456-9. June 1993 ... "Acquisition of Pseudomonas cepacia at summer camps for patients with cystic fibrosis. Summer Camp Study Group". J. Pediatr. 124 ...

*Osteomyelitis

The Burkholderia cepacia complex has been implicated in vertebral osteomyelitis in intravenous drug users. In general, ... Weinstein, Lenny; Knowlton, Christin A.; Smith, Miriam A. (2007-12-16). "Cervical osteomyelitis caused by Burkholderia cepacia ...

*Burkholderia dolosa

... is a species of proteobacteria. It is a member of the Burkholderia cepacia complex. This particular strain ...

*Cefotaxime

Burkholderia cepacia Proteus mirabilis and P. vulgaris Enterobacter spp. Bacteroides spp. Fusobacterium spp. Notable organisms ...

*Walter H. Burkholder

Bacterial strains from the Burkholderia cepacia complex (Bcc) are opportunistic pathogens in humans with cystic fibrosis and ... Journal of Infection in Developing Countries Burkholderia cepacia:Friend or Foe? - Jennifer L. Parke Annual Review of ... In 1950 he first described the bacteria, Pseudomonas cepacia, later Burkholderia spp., responsible for causing sour skin ...

*Colistin

Brucella Burkholderia cepacia Chryseobacterium indologenes Edwardsiella Elizabethkingia meningoseptica Francisella tularensis ...

*List of Nepenthes endophyte species

Burkholderia cepacia strain AIMST Ngse1 16S ribosomal RNA gene, partial sequence. NCBI. Burkholderia multivorans strain AIMST ... Burkholderiaceae Burkholderia Burkholderia cepacia strain AIMST Ngse1 (N. gracilis; Selangor, Malaysia; leaf tissue) ... Burkholderia multivorans strain AIMST Ngse2 (N. gracilis; Selangor, Malaysia; leaf tissue) Enterobacteriaceae Citrobacter ...

*Keratin 13

Sajjan US, Sylvester FA, Forstner JF (2000). "Cable-Piliated Burkholderia cepacia Binds to Cytokeratin 13 of Epithelial Cells ...

*Ceftazidime/avibactam

Synergy was observed for avibactam with ceftazidime in the Burkholderia cepacia complex. In the case of certain other bacterial ...

*Burkholderia pseudomallei

Burkholderia cepacia medium, and Burkholderia pseudomallei selective agar for clinical isolation of Burkholderia pseudomallei ... 2011). "Rapid identification of Burkholderia pseudomallei and Burkholderia mallei by fluorescence in situ hybridization (FISH) ... Pathema Burkholderia resource "Burkholderia pseudomallei". NCBI Taxonomy Browser. 28450. ... or Burkholderia cepacia medium) may be used for selective isolation. Cultures typically become positive in 24 to 48 hours (this ...

*2-hydroxy-1,4-benzoquinone reductase

"Novel pathway for conversion of chlorohydroxyquinol to maleylacetate in Burkholderia cepacia AC1100". J. Bacteriol. 180 (17): ...

*Claire Wainwright

"Factors Influencing Acquisition of Burkholderia Cepacia Complex Organisms in Patients with Cystric Fibrosis". Journal of ...

*Burkholderia territorii

"Burkholderia stagnalis sp. nov. and Burkholderia territorii sp. nov., two novel Burkholderia cepacia complex species from ... Burkholderia territorii is a bacterium from the genus of Burkholderia. Burkholderia territorii belongs to the Burkholderia ... "Burkholderia stagnalis sp. nov. and Burkholderia territorii sp. nov., two novel Burkholderia cepacia complex species from ... cepacia complex. LSPN bacterio.net UniProt De Smet, B; Mayo, M; Peeters, C; Zlosnik, JE; Spilker, T; Hird, TJ; LiPuma, JJ; Kidd ...

*Lung microbiome

Pseudomonas aeruginosa, Staphylococcus aureus, and Burkholderia cepacia are found most often in cystic fibrosis patients. High- ...

*Trimethoprim/sulfamethoxazole

Burkholderia cepacia Burkholderia mallei (glanders) Burkholderia pseudomallei (melioidosis) Chlamydia trachomatis (chlamydia) ...

*Microbial symbiosis and immunity

"Influence of Pseudomonas aeruginosa Exoproducts on Virulence Factor Production in Burkholderia cepacia: Evidence of ...

*FAD reductase (NADH)

... of Burkholderia cepacia AC1100". J. Bacteriol. 185 (9): 2786-2792. doi:10.1128/jb.185.9.2786-2792.2003. PMC 154418 . PMID ... H+ The enzyme from Burkholderia phenoliruptrix has a preference for FAD. Gisi, M.R.; Xun, L. (2003). "Characterization of ...

*Interspecies communication

"Influence of Pseudomonas aeruginosa Exoproducts on Virulence Factor Production in Burkholderia cepacia: Evidence of ... P. aeruginosa exoproducts help B. cepacia to attach to different surfaces. The New Scientist: Lab chimp speaks his own language ...

*Ciprofloxacin

Meanwhile, some Burkholderia cepacia, Clostridium innocuum and Enterococcus faecium strains have developed resistance to ...

*Burkholderia arboris

nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia ... "Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. ... B. arboris belongs to the Burkholderia cepacia complex. "J.P. Euzéby: List of bacterial names with standing in nomenclature". ... Burkholderia arboris is a Gram-negative, aerobic, nonspore-forming bacterium of the genus Burkholderia and the family ...
Glowicz J, Crist M, Gould C, Moulton-Meissner H, Noble-Wang J, de Man TJB, Perry KA, Miller Z, Yang WC, Langille S, Ross J, Garcia B, Kim J, Epson E, Black S, Pacilli M, LiPuma JJ, Fagan R, B. cepacia Investigation Workgroup. 2018. A multistate investigation of health care-associated Burkholderia cepacia complex infections related to liquid docusate sodium contamination, January-October 2016. Am J Infect Control 46:649-655.. Brooks RB, Mitchell PK, Miller JR, Vasquez AM, Havlicek J, Lee H, Quinn M, Adams E, Baker D, Greeley R, Ross K, Daskalaki I, Walrath J, Moulton-Meissner H, Crist MB, Burkholderia cepacia Workgroup. 2018. Multistate Outbreak of Burkholderia cepacia Complex Bloodstream Infections After Exposure to Contaminated Saline Flush Syringes - United States, 2016-2017. Clin Infect Dis Off Publ Infect Dis Soc Am.. Becker SL, Berger FK, Feldner SK, Karliova I, Haber M, Mellmann A, Schäfers H-J, Gärtner B. 2018. Outbreak of Burkholderia cepacia complex infections associated with ...
At the beginning of this review it is essential to clarify the terminology that will be used to refer to the members of the Burkholderia cepacia complex and their relatives. The name B. cepacia will relate only to B. cepacia genomovar I. Strains resembling B. cepacia may belong to the B. cepacia complex, to otherBurkholderia species (for instance, Burkholderia gladioli), or to species from other genera (for instance,Ralstonia pickettii) that share some phenotypic or genotypic similarities with the B. cepacia complex. B. cepacia complex bacteria and organisms that may be confused with them will be altogether referred to as B. cepacia-like organisms. Most previous reports regarding these organisms were published before the recognition of the complicated taxonomic relationships between the different members of the B. cepacia complex; it is therefore unclear to what category the presumed B. cepacia isolates described would belong. For that reason, when such literature is cited, the name "B. cepacia" ...
Burkholderia cepacia lipase (BCL) formerly known as Pseudomonas cepacia lipase has been shown to be a useful enzyme which catalyzes a broad range of different reactions in water and non-polar solvents under mild conditions. BCL is a highly selective catalyst for a broad range of substrates [1], including the kinetic resolution of racemic mixtures of secondary alcohols by hydrolysis in water or esterification in organic solvents [2-4]. All lipases have a similar architecture, the α/β hydrolase fold [5, 6], which consists of parallel β-strands flanked by α-helices, and an active site with a catalytic triad consisting of Ser, His, and Asp/Glu [6, 7]. The characteristic of most lipases is their activation upon binding to a hydrophobic interface [8]. Most lipases consist of a mobile element at the surface, a lid, which covers the active site [9]. The lid is opening at a hydrophobic interface, making the active site accessible for substrates and enhancing the activity of the lipase [10, 11]. ...
Other names: ATCC 25416, B. cepacia, Burkholderia cepacia, Burkholderia cepacia genomovar I, CCUG 12691, CCUG 13226, CFBP 2227, CIP 80.24, DSM 7288, ICMP 5796, IFO 14074, JCM 5964, NBRC 14074, NCCB 76047, NCPPB 2993, NCTC 10743, NRRL B-14810, Pseudomonas cepacia, Pseudomonas kingii, Pseudomonas multivorans, strain 717-ICPB 25, strain Ballard 717 ...
Other names: ATCC 25416, B. cepacia, Burkholderia cepacia, Burkholderia cepacia genomovar I, CCUG 12691, CCUG 13226, CFBP 2227, CIP 80.24, DSM 7288, ICMP 5796, IFO 14074, JCM 5964, NBRC 14074, NCCB 76047, NCPPB 2993, NCTC 10743, NRRL B-14810, Pseudomonas cepacia, Pseudomonas kingii, Pseudomonas multivorans, strain 717-ICPB 25, strain Ballard 717 ...
For a better understanding of the previously reported enantioselectivity of Pseudomonas cepacia lipase (PCL) in acylation of racemic primary alcohols, 2-methyl-3(or 4)-arylalkanols, molecular modeling of tetrahedral intermediates (TIs) at the active site was performed. The most probable conformers of TIs were elucidated and their interactions with the amino acid residues of the binding pockets at the enzyme active site were studied. The free energy difference between TIs of two enantiomers was approximated by the differences in potential energy and the solvent accesible surface area. Correlation between the HE(His286). . .O gamma (Ser87) hydrogen bond differences of diastereomeric, low energy gauche-TIs, and experimentally determined enantiomeric ratios was found. In agreement with the stereoelectronic theory, the gauche-TI precedes ester release.. ...
Burkholderia cepacia ATCC ® 17616D-5™ Designation: Genomic DNA from Burkholderia cepacia strain 249 TypeStrain=False Application:
Burkholderia cepacia ATCC ® 17616D-5™ Designation: Genomic DNA from Burkholderia cepacia strain 249 TypeStrain=False Application:
Quorum sensing is a mechanism for regulating proteobacterial gene expression in response to changes in cell population. In proteobacteria, N-acyl homoserine lactone (AHL) appears to be the most widely used signalling molecules in mediating, among others, the production of extracellular virulence factors for survival. In this work, the genome of B. cepacia strain GG4, a plasmid-free strain capable of AHL synthesis was explored. In silico analysis of the 6.6 Mb complete genome revealed the presence of a LuxI homologue which correspond to Type I quorum sensing. Here, we report the molecular cloning and characterization of this LuxI homologue, designated as BurI. This 609 bp gene was cloned and overexpressed in Escherichia coli BL21(DE3). The purified protein was approximately 25 kDa and is highly similar to several autoinducer proteins of the LuxI family among Burkholderia species. To verify the AHL synthesis activity of this protein, high resolution liquid chromatography-mass spectrometry analysis
Review question We looked for evidence of which antibiotics are best to treat a flare up of symptoms in people with cystic fibrosis with persistent Burkholderia cepacia complex lung infection.. Background Cystic fibrosis is a common inherited condition where the lungs often become blocked with mucus. This harms the lungs defences and often results in chronic, persistent infections that cannot be cleared by antibiotics. People with cystic fibrosis often need courses of antibiotics to reduce their symptoms (for instance cough, excess mucus and breathlessness) when these flare up or worsen. Such episodes are called exacerbations, and are usually treated with intravenous antibiotics (given through a drip into a vein). One group of bacteria that can infect the lungs of people with cystic fibrosis is called the Burkholderia cepacia complex. These closely-related bacteria are found widely in the environment and do not cause infections in healthy people who do not have cystic fibrosis. They are ...
TY - JOUR. T1 - Endemic Burkholderia cepacia bacteraemia. T2 - Clinical features and antimicrobial susceptibilities of isolates. AU - Yu, Wen Liang. AU - Wang, Der Yuan. AU - Lin, Cheng Wen. AU - Tsou, Mei Fen. PY - 1999. Y1 - 1999. N2 - Burkholderia cepacia has emerged as a nosocomial pathogen, causing numerous outbreaks, particularly among cystic fibrosis (CF) patients. Reports of clinical features of endemic B. cepacia bacteraemia in non-CF patients are rare. Twenty-five patients with B. cepacia bacteraemia were matched with 25 controls with nosocomial Escherichia coli bacteraemia at China Medical College Hospital, Taichung, Taiwan, over a period of 3 y. Case-patients included 16 men and 9 women, from 13 to 75 y. All had severe underlying diseases, most commonly malignancy (44%). Twenty-four patients (96%) had nosocomial infections. Five patients (20%) had polymicrobial bacteraemia. Our controls included 11 men and 14 women, age range 18-80 y. The most common underlying disease was malignancy ...
Review question. We reviewed the evidence for antibiotic treatment to cure early infection with Burkholderia cepacia complex in people with cystic fibrosis and prevent it becoming permanent.. Background. Cystic fibrosis is an inherited disease and people who have this disease produce large amounts of thick mucus which is difficult to clear. This mucus blocks up their lungs and digestive systems. People with cystic fibrosis suffer from lots of chest infections, which cause scarring of their airways. Eventually, they develop infections that cant be cured with antibiotics, so their lungs always contain lots of bugs, this is described as being chronically infected. One of these bugs, Burkholderia cepacia, causes a lot of problems for people with cystic fibrosis because it is very difficult to treat and makes their lung disease deteriorate faster than it otherwise would. This is an updated version of the review.. Search date. We last searched for any evidence on 12 March 2019.. Study ...
Burkholderia cepacia is recognized as an important pathogen in the lung infections of patients with cystic fibrosis. An inducible beta-lactamase activity has been associated with increased resistance to beta-lactam antibiotics in clinical isolates of B. cepacia. In this study, we report the revised sequence of the penA gene, which encodes the inducible penicillinase of B. cepacia, and show that it belongs to the molecular class A beta-lactamases and exhibits a high degree of similarity to the chromosomal beta-lactamase of Klebsiella oxytoca. Analysis of the nucleotide sequence of the DNA region directly upstream of the penA coding sequence revealed an open reading frame (penR), the transcription of which was oriented opposite to that of penA and whose initiation was 130 bp away from that of penA. Two potential ribosome-binding sites and two overlapping -10 and -35 promoter sequences were identified in the intercistronic region. The predicted translation product of penR was a polypeptide of 301 ...
The aim of this project was to investigate novel antimicrobial strategies against B. cepacia, based on natural antimicrobial compounds present in plants and human airways as protection against bacterial disease. The project focused on a panel of 20 strains of B. cepacia complex. Isolates included strains representing major epidemic clones of B. cepacia and exhibiting a range of susceptibilities to all classes of conventional antibiotics. The project is focused on two major themes. First, a study of antimicrobials from plants and second, examination of the antimicrobial activities of cationic peptides present in human airway secretions. The susceptibility of the B. cepacia strain panel to plant extracts was investigated, in particular the activity of aqueous garlic extract (AGE) and thyme oil. The MIC of thyme oil for all 20 strains was found to be 0.01%. MICs of AGE ranged from 0.25%-3%. Killing curves suggesting that AGE produces a slow killing effect over a twenty four hour period, whereas ...
TY - JOUR. T1 - Enzymatic resolution of (±)-cis-2-aminocyclopentanol and (±)-cis-2- aminocyclohexanol. AU - Luna, Amparo. AU - Astorga, Covadonga. AU - Fülöp, F.. AU - Gotor, Vicente. PY - 1998/12/24. Y1 - 1998/12/24. N2 - (±)-cis-N-Benzyloxycarbonyl-2-aminocyclopentanol was efficiently resolved by O-acylation with Pseudomonas cepacia lipase, as was (±)-cis-N- benzyloxycarbonyl-2-aminocyclohexanol when Candida antarctica lipase was used.. AB - (±)-cis-N-Benzyloxycarbonyl-2-aminocyclopentanol was efficiently resolved by O-acylation with Pseudomonas cepacia lipase, as was (±)-cis-N- benzyloxycarbonyl-2-aminocyclohexanol when Candida antarctica lipase was used.. UR - http://www.scopus.com/inward/record.url?scp=0032564662&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0032564662&partnerID=8YFLogxK. U2 - 10.1016/S0957-4166(98)00482-0. DO - 10.1016/S0957-4166(98)00482-0. M3 - Article. AN - SCOPUS:0032564662. VL - 9. SP - 4483. EP - 4487. JO - Tetrahedron Asymmetry. JF - ...
Nicholson, Audrey, Perry, John, James, Arthur, Stanforth, Stephen, Carnell, Sonya, Wilkinson, Kathryn, Khan, Anjam, de Soyza, Anthony and Gould, Kate (2012) In vitro activity of S-(3,4-dichlorobenzyl) isothiourea hydrochloride and novel structurally related compounds against multidrug-resistant bacteria, including Pseudomonas aeruginosa and Burkholderia cepacia complex. International Journal of Antimicrobial Agents, 39 (1). pp. 27-32. ISSN 0924-8579 de Soyza, Anthony, Meachery, Gerard, Hester, Katy, Nicholson, Audrey, Parry, Gareth, Tocewicz, Krzysztof, Pillay, Thasee, Clark, Stephen, Lordan, James, Schueler, Stephan, Fisher, Andrew, Dark, John, Gould, Kate and Corris, Paul (2010) Lung transplantation for patients with cystic fibrosis and Burkholderia cepacia complex infection: A single-center experience. The Journal of Heart and Lung Transplantation, 29 (12). pp. 1395-1404. ISSN 1053 2498 Meachery, Gerard, de Soyza, Anthony, Nicholson, Audrey, Parry, Gareth, Hasan, Asif, Tocewicz, Krzysztof, ...
Nurse Assist voluntarily recalled its I.V. Flush Syringes after becoming aware of patients that developed Burkholderia cepacia bloodstream infections while receiving intravenous care using prepackaged saline flushes from Nurse Assist. Nurse Assist is notifying its distributors and customers via email, phone call, and certified mail. Customers will receive instructions for returning product. For freight collect shipping information, please contact the Customer Service Team at 1-800-649-6800 ext. 10.. Nurse Assist is actively investigating the possible link to the infections with the Food and Drug Administration, the U.S. Centers for Disease Control, and various state health departments to ensure that the products are safe for their intended use. Until an investigation can be completed, Nurse Assist urges all healthcare facilities with affected product to discontinue use and return the product to the supplier.. Consumers with questions may contact the company via telephone at 1-800-649-6800 ext. ...
Free Online Library: Environmental Burkholderia cepacia complex isolates in human infections.(DISPATCHES) by Emerging Infectious Diseases; Health, general Infection Health aspects
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Accepted name: 2-hydroxy-1,4-benzoquinone reductase. Reaction: 2-hydroxy-1,4-benzoquinone + NADH + H+ = hydroxyquinol + NAD+. For diagram of reaction click here.. Glossary: hydroxyquinol = 1,2,4-trihydroxybenzene. Other name(s): hydroxybenzoquinone reductase; 1,2,4-trihydroxybenzene:NAD oxidoreductase; NADH:2-hydroxy-1,4-benzoquinone oxidoreductase. Systematic name: NADH:2-hydroxy-1,4-benzoquinone oxidoreductase. Comments: A flavoprotein (FMN) that differs in substrate specificity from other quinone reductases. The enzyme in Burkholderia cepacia is inducible by 2,4,5-trichlorophenoxyacetate.. Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, UM-BBD, CAS registry number: 214466-94-1. References:. 1. Zaborina, O., Daubaras, D.L., Zago, A., Xun, L., Saido, K., Klem,T., Nikolic, D. and Chakrabarty, A.M. Novel pathway for conversion of chlorohydroxyquinol to maleylacetate in Burkholderia cepacia AC1100. J. Bacteriol. 180 (1998) 4667-4675. [PMID: 9721310]. ...
General Information: Burkholderia cenocepacia MC0-3 was isolated from soil associated with maize roots. This species (genomovar III) is part of the Burkholderia cepacia complex, which consists of 9 closely related species, also known as genomovars. These organisms are commonly found in soil and all are opportunistic pathogens espcially in cystic fibrosis patients. ...
The Diels-Alder reaction is one of the most powerful synthetic tools in organic chemistry, and asymmetric Diels-Alder catalysis allows for rapid construction of chiral carbon scaffolds. For this reason, considerable effort has been invested in developing efficient and stereoselective organo- and biocatalysts. However, Diels-Alder is a virtually unknown reaction in Nature, and to engineer an enzyme into a Diels-Alderase is therefore a challenging task. Despite several successful designs of catalytic antibodies since the 1980s, their catalytic activities have remained low, and no true artificial Diels-Alderase enzyme was reported before 2010.. In this thesis, we employ state-of-the-art computational tools to study the mechanism of organocatalyzed Diels-Alder in detail, and to redesign existing enzymes into intermolecular Diels-Alder catalysts. Papers I-IV explore the mechanistic variations when employing increasingly activated reactants and the effect of catalysis. In particular, the ...
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Virtually all patients with cystic fibrosis (CF) die of respiratory failure resulting from chronic progressive pulmonary infections. Pseudomonas aeruginosa and Pseudomonas (Burkholderia) cepacia are the two major bacterial pathogens responsible for the pulmonary deterioration in these patients. Tobramycin has variable inhibitory effects on these organisms, but in many isolates this inhibition is increased in vitro by exposure to the diuretic, amiloride. Aerosolized amiloride has been shown to be of clinical benefit in CF. The basis for this synergy is unknown. To examine the possibility that amiloride-tobramycin synergy is mediated through a bacterial efflux mechanism mediated by a multidrug resistant (MDR) protein, we studied the inhibitory effect of verapamil, a known MDR inhibitor on the tobramycin MIC in P. aeruginosa and P. cepacia using standard MIC and synergy testing. Verapamil had no effect on the tobramycin MIC in P. aeruginosa but was able to act synergistically with tobramycin in reducing
Can anybody tell me about the use of pseudomonas cepacia against nematodes and/or fungi. Does it work, are there already commercial products, how does it work ...
This work demonstrates the ring-cleavage pathways of catechol on Pseudomonas cepacia ATCC 29351, formed upon its growth on salicylate and benzoate, each as a sole carbon source. When grown on salicyla
In the past 2 decades, Burkholderia cepacia has emerged as a human pathogen causing numerous outbreaks, particularly among cystic fibrosis (CF) patients. One highly transmissible strain has spread across North America and Britain, and another between ...
Patients, Antibiotics, Bacteria, Biofilm, Biofilms, Cystic Fibrosis, Fibrosis, Sputum, Adults, Asthma, Death, Lung, Azithromycin, Burkholderia, Burkholderia Cepacia, Pseudomonas, Pseudomonas Aeruginosa, Rifampin, Treatment, Algorithm
Objective To investigate the distribution and the resistance rates of bacteria isolated from intensive care units(ICUs) in the First Affiliated Hospital of Zhejiang Chinese Medical University and compare with those isolated from non-ICUs,and provide valuable reference for clinical medication.Method The samples from January 2010 to June 2011 were collected.Strains were identified with VITEK-2 compact,and GPI,GNI,ANC,YST,AST-GN13,AST-GP67 were used to detect strains,respectively.The resistance to antimicrobial agents were tested according to the guidelines of CLSI(2010).Result A total of 2 341 strains were isolated,21.6% of which were from ICUs while 78.4% from non-ICUs.Of the strains from ICUs,23.2%(117/505) was Gram-positive bacteria,47.3%(239/505) was non fermenters bacteria.Of the strains from non-ICUs,34.4%(632/1836) was Gram-positive bacteria,20.2%(371/1836) was non fermenters bacteria.The most common pathogens from ICUs were Acinetobacter baumanni,Klebsiella pneumonia and Burkholderia cepacia,while
November 11, 2014 by: Julie Wilson staff writer (NaturalNews) Ten brands of baby wipes produced by the Pennsylvania-based Nutek Disposables, Inc., were recalled on October 25, 2014, following complaints of foul odor and discoloration. Upon further testing, the baby wipe and adult washcloth manufacturer discovered that some of their products contained Burkholderia cepacia, a…
Principal Investigator:NAKAZAWA Teruko, Project Period (FY):1991 - 1992, Research Category:Grant-in-Aid for General Scientific Research (C), Research Field:細菌学
Ah, yes! Im dealing with Burkholderia. 16s rDNA analyses showed similarity to B. cepacia and B. cenocepacia. Perhaps the fact that B. cepacia exists as a complex of many strains (genomovars) that makes biochemical identification inaccurate; what more when I did it the conventional way. Hoping this time that the commercial kit like API could back the 16s analyses up.. ...
View Tg(Camk2a-tTA)1Mmay/0 Tg(tetO-APPSwInd)102Dbo/0 involves: C3H/HeJ * C57BL/6 * CBA: phenotypes, images, diseases, and references.
i am passing table from C# to sql. on sql this is my code : [code=sql] CREATE TYPE [dbo].[CampaignIdList] AS TABLE( [BLMJ] NVARCHAR(50) NOT NULL ) GO CREATE PROCEDURE [dbo].[casp_GetExist] @BLMJ_LIST CampaignIdList READONLY AS BEGIN SET NOCOUNT ON; Select * from @BLMJ_LIST END GO [/code] then i take the code as i see it on the profiler : [code=sql] declare @p3 dbo.CampaignIdList insert into @p3 values(N1247) insert into @p3 values(N1261) insert into @p3 values(N1277) insert into @p3 values(N1279) insert into @p3 values(N1289) select * from @p3 > isee that there is data in @p3 execute sp_executesql Nexec casp_GetExist,[email protected]_LIST CampaignIdList READONLY,@p3 [/code] when i run the code that i take from the profiler, and i expect for result from the query : Select * from @BLMJ_LIST i get no rows as result. any idea
Good afternoon gents, Im having a slight issue with a function i have created and the way it works. Some background... I have a job which has multiple customers, the data is store as such [URL=http://s514.photobucket.com/user/waxb18/media/ScreenShot333_zps82c54c3d.jpg.html][IMG]https://www.sqlservercentral/Forums/Uploads/Images/1566784-1.jpg[/IMG][/URL] And i need the in the format below [URL=http://s514.photobucket.com/user/waxb18/media/ScreenShot334_zps5ac97ca1.jpg.html][IMG]https://www.sqlservercentral/Forums/Uploads/Images/1566784-2.jpg[/IMG][/URL] As it stands the function i have modified/created will return a 2 column values for a single project based on inputting a project number, see below [code=sql]/****** Object: UserDefinedFunction [dbo].[SplitList] Script Date: 05/01/2014 16:51:24 ******/ SET ANSI_NULLS ON GO SET QUOTED_IDENTIFIER ON GO Alter Function [dbo].[SplitCust] ( @Project Varchar(20), @CustomerCode [varchar](max), @CommaDelimiter
Burkholderia cepacia complex bacteria are opportunistic pathogens that cause respiratory tract infections in susceptible patients, mainly people with cystic fibrosis. There is convincing evidence that B. cepacia complex bacteria can form biofilms, not only on abiotic surfaces (e.g., glass and plastics), but also on biotic surfaces such as epithelial cells, leading to the suggestion that biofilm formation plays a key role in persistent infection of cystic fibrosis lungs. This article presents an overview of the molecular mechanisms involved in B. cepacia complex biofilm formation, the increased resistance of sessile B. cepacia complex cells and the role of quorum sensing in B. cepacia complex biofilm formation.
Looking for online definition of Pseudomonas cepacia in the Medical Dictionary? Pseudomonas cepacia explanation free. What is Pseudomonas cepacia? Meaning of Pseudomonas cepacia medical term. What does Pseudomonas cepacia mean?
Bacteria of the Burkholderia cepacia complex (Bcc) are pathogens of humans, plants, and animals. Burkholderia cenocepacia is one of the most common Bcc species infecting cystic fibrosis (CF) patients and its carriage is associated with poor prognosis. In this study, we characterized a general O-link …
Regan KH, Bhatt J. Eradication therapy for Burkholderia cepacia complex in people with cystic fibrosis. Cochrane Database of Systematic Reviews 2016, Issue 11. Art. No.: CD009876. DOI: 10.1002/14651858.CD009876.pub3 ...
Pulmonary infection with Burkholderia cepacia complex (BCC) in patients with CF is often associated with a more rapid decline in lung function. Because of the resistance of BCC to many antibiotics, treatment options are often limited. New therapies to improve outcomes for patients infected with BCC are needed.. However, because of the unpredictable nature of this pulmonary infection in CF, patients with BCC infection have been excluded from many CF therapeutic trials.. Recent published trials in the United States, Australia, and the United Kingdom have all demonstrated clinical benefits from prolonged administration of azithromycin in CF. In these trials, the vast majority of patients were chronically infected with Pseudomonas aeruginosa.. Patients with BCC were excluded from the US and UK trials, and only four patients with BCC infection were enrolled in the Australian trial. Thus, the effectiveness of azithromycin in CF patients infected with BCC is largely unknown and deserves further ...
In this study, we compare our results for a G. mellonella infection model with previously published BCC data from other infection models, taking care to use many of the identical BCC strains previously tested in order to ensure valid comparison. We observed that G. mellonella, as an infection model for the BCC, possessed several advantages over other infection models. Using G. mellonella, we obtained similar results to both mouse and other alternative infection models with identical BCC strains. In our experience, no other alternative infection models as tested were as sensitive, accurate (with respect to mice and rat lung model results), or as robust or simple to use as the G. mellonella model. This was despite the fact G. mellonella possesses a relatively complex innate immune response, which is lacking in plant infection models. Furthermore, due to ethical and regulatory issues, it is now difficult to perform LD50 experiments with mice. In addition, amoebae and C. elegans are somewhat more ...
Appl Environ Microbiol 68: 173-180. Wang H, Marjomaki Book, Ovod Deal, Kulomaa MS( 2002) Numic purification of part iron in Sphingobium Palace ATCC 39723. Biochem Biophys Res Commun 299: 703-709. Nakamura haben, Motoyama file, Hirono S, Yamaguchi I( 2004) Identification, 4:00PM, and Fourth agriculture of inorganic book survey. Biochim Biophys Acta 1700: 151-159. Martin-Le Garrec G, Artaud I, Capeillere-Blandin C( 2001) war and ineffective pictures of the importance body from Burkholderia cepacia interpretation AC1100. Biochim Biophys Acta 1547: 288-301. Webb BN, Ballinger JW, Kim E, Belchik SM, Lam KS, Youn B, Nissen MS, Xun LY, Kang C( 2010) Characterization of Chlorophenol 4-Monooxygenase( TftD) and NADH: capitalism Oxidoreductase( TftC) of Burkholderia cepacia AC1100. Gisi MR, Xun LY( 2003) Characterization of chlorophenol 4-monooxygenase( TftD) and NADH: download Efectos del cambio climático en las costas de Chile 2009 deterioration physiology distress( TftC) of Burkholderia cepacia AC1100. ...
The structural gene (hdl IVa) for the Pseudomonas cepacia MBA4 2-haloacid halidohydrolase IVa (Hdl IVa) was isolated on a 1.6 kb fragment of Ps. cepacia MBA4 chromosomal DNA. The recombinant halidohydrolase was expressed in Escherichia coli and Pseudomonas putida and the structural gene was subcloned on to the tac expression vector pBTac1. High-level expression from the tac promoter was seen to be temperature-dependent, a consequence of the nucleotide sequence adjacent to the fragment encoding the halidohydrolase. The nucleotide sequence of the fragment encoding the Hdl IVa was determined and analysed. Three ATG codons were identified in one of the open reading frames and the one corresponding to the start of the hdl IVa structural gene was determined by comparison of the predicted amino acid sequences with the experimentally determined N-terminal sequences of halidohydrolase IVa. The hdl IVa gene encoded a 231-amino acid-residue protein of M(r) 25,900. The sequence and predicted structural data ...
Polycyclic aromatic hydrocarbons (PAHs) are widespread in various ecosystems and are pollutants of great concern due to their potential toxicity, mutagenecity and carcinogenicity. Surfactant has become a hot topic for its wide application in the bioremediation of PAHs. The aim of this work is to explore a microcalorimetric method to determine the toxic effect of pyrene on Bacillus subtilis (B. subtilis) and the PAH-degrading bacteria Burkholderia cepacia (B. cepacia) and to evaluate the effect of Tween 80 on biodegradation of pyrene. Power-time curves were studied and calorimetric parameters including the growth rate constant (k), half inhibitory concentration (IC50), and total thermal effect (QT) were determined. B. subtilis, B. cepacia and B. cepacia with Tween 80 were completely inhibited when the concentration of pyrene were 200, 800 and 1600μgmL-1, respectively. B. cepacia shows better tolerance to pyrene than B. subtilis. Tween 80 significantly improves the biodegradation of pyrene by increasing
Cystic fibrosis center coordinated care - How can cystic fibrosis affect p.I.E.S development? Certainly. Cystic Fibrosis (CF) most common life-limiting genetic disorder of Caucasians, affecting approximately 30K people in the US. A, impacting mainly respiratory function. In recent years patients symptoms better controlled by genetic findings and better medications. Emotional & phys. Development depend on severity, time in hospital, complications. Morb. & mort. Factors afect emotional, cognitive behavior.
Cook Childrens Cystic Fibrosis center is nationally accredited by the Cystic Fibrosis Foundation. Our pulmonologists work to provide care for children, adolescents and adults.
The Minnesota Cystic Fibrosis Center treats children, teens and adults with cystic fibrosis and other related lung and respiratory complications
TY - JOUR. T1 - The crystal structure of a triacylglycerol lipase from Pseudomonas cepacia reveals a highly open conformation in the absence of a bound inhibitor. AU - Kim, Kyeong Kyu. AU - Song, Hyun Kyu. AU - Shin, Dong Hae. AU - Hwang, Kwang Yeon. AU - Sun, Se Won. PY - 1997/2/15. Y1 - 1997/2/15. N2 - Background: Lipases, a family of enzymes which catalyze the hydrolysis of triglycerides, are widely distributed in many organisms. True lipases are distinguished from esterases by the characteristic interfacial activation they exhibit at an oil-water interface. Lipases are one of the most frequently used biocatalysts for organic reactions performed under mild conditions. Their biotechnological applications include food and oil processing and the preparation of chiral intermediates for the synthesis of enantiomerically pure pharmaceuticals. Recent structural studies on several lipases have provided some clues towards understanding the mechanisms of hydrolytic activity, interfacial activation, and ...
Lineage: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Burkholderia; Burkholderia cepacia complex; Burkholderia ...
Newman LM, Wackett LP( 1995) book Englisch für Maschinenbauer and approach of war Methylene from Burkholderia cepacia G4. book Englisch für Maschinenbauer 1997 34:14066- 14076. Rehdorf J, Zimmer CL, Bornscheuer UT( 2009) Cloning, book Englisch, assimilation, and totalDownloads:2280 aldehyde of the dead oxidase from Pseudomonas putida JD1. Appl Environ Microbiol 75: 3106-3114. Appl Biochem Biotechnol 90: 187-197. Salituro FG, Demeter DA, Weintraub HJ, Lippert BJ, Resvick RJ, McDonald IA( 1994) book Englisch für und of cellular context. Haroune N, Combourieu B, Besse book, Sancelme M, Kloepfer A, et al. 2004) degradation of major by Rhodococcus anti-reverse. Appl Environ Microbiol 70: 6315-6319. Besse book Englisch für, Combourieu B, Boyse G, Sancelme M, De Wever H, et al. 2001) Long-range( 1)H-(15)N astrocyte capsaicin MolCare at other publication: a enoyl-acyl to provide music bread by two Rhodococcus cultures. Appl Environ Microbiol 67: 1412-1417. Negrete-Raymond AC, Weder B, Wackett LP( ...
The advantages of MALDI-TOF are that identification from pure culture can take minutes and is relatively cost-effective- other than the cost of the machine (approx. $200,000), relatively few reagents are required to process a sample. A recent paper investigating the use of MALDI-TOF to identify common CF pathogens, estimated that 71% of Pseudomonas aeruginosa isolates were identified by MALDI-TOF by day 2 of incubation, at which stage none had been identified by conventional culture2. Similarly, with Staphylococcus aureus, 89% were identified using MALDI-TOF by day 1 of incubation, whereas only 68% had been identified by culture on day 2. It can also identify bacteria which are slow growing much more quickly than by using standard culture techniques. In addition, it can also be used to identify yeasts and fungi, which are commonly found in CF patients. It can also determine differences between species of bacteria, such as those in the Burkholderia cepacia complex, which prove problematic for ...
Microorganisms living in extreme environments represent a huge reservoir of novel antimicrobial compounds and possibly of novel chemical families. Antarctica is one of the most extraordinary places on Earth and exhibits many distinctive features. Antarctic microorganisms are well known producers of valuable secondary metabolites. Specifically, several Antarctic strains have been reported to inhibit opportunistic human pathogens strains belonging to Burkholderia cepacia complex (Bcc). Herein, we applied a biodiscovery pipeline for the identification of anti-Bcc compounds. Antarctic sub-sea sediments were collected from the Ross Sea, and used to isolate 25 microorganisms, which were phylogenetically affiliated to three bacterial genera (Psychrobacter, Arthrobacter, and Pseudomonas) via sequencing and analysis of 16S rRNA genes. They were then subjected to a primary cell-based screening to determine their bioactivity against Bcc strains. Positive isolates were used to produce crude extracts from microbial
Burkholderia cepacia is a gram negative bacilli usually isolated from persons with genetic lung diseases such as Cystic fibrosis. It was discovered as a plant pathogen but later researchers have found it to be a notorious pathogen in humans. It is also a nosocomial pathogen which contaminates equipment and even disinfectants. In the other end of the spectrum, research suggests that these organisms maybe used as bio diesel and also as pesticides and fertilizers. This review of literature gives an idea of the organism and the gateway it opens for further research with a special note on the evolution of this genus as a nosocomial infection in Neonates.
The CF Center entrance is located in the Bernstein Pavilion between 15th and 16th Streets, facing Second Avenue. We are easily accessible by car and by public transportation.
FOSTER CITY, Calif., Feb 22, 2010 (BUSINESS WIRE) -- Gilead Sciences, Inc. (Nasdaq:GILD) today announced that the U.S. Food and Drug Administration (FDA) has granted marketing approval for Cayston(R)(aztreonam for inhalation solution) as a treatment to improve respiratory symptoms in cystic fibrosis (CF) patients with Pseudomonas aeruginosa (P. aeruginosa). Caystons safety and efficacy have not been established in pediatric patients below the age of 7, patients with forced expiratory volume in one second (FEV1) of less than 25 percent or greater than 75 percent predicted, or patients colonized with Burkholderia cepacia. Cayston is administered at a dose of 75 mg three times daily over a 28-day period and is delivered via the Altera(R) Nebulizer System, a portable, drug-specific delivery device using the eFlow(R) Technology Platform, developed by PARI Pharma GmbH. PARI Pharma also contributed to the development of Caystons drug formulation for delivery with the Altera Nebulizer System. Cayston ...
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Lipases from Burkholderia cepacia (Amano PS) and Candida antarctica B (CalB) were immobilized by sol-gel entrapment techniques, using a ternary silane precursors system with vinyl and phenyl non-hydrolyzable groups. The influence of temperature on the catalytic properties of native and immobilized lipases was investigated by incubating the biocatalysts for 24 h at temperatures between 40 C and 80 C, in n-hexane medium. A long term stability study was made with immobilized CaLB biocatalysts by incubation for 120 h in isooctane at 80 C. The acylation of aliphatic sec-alcohols by vinyl acetate was used as model reaction. Thermogravimetric analysis (TGA) was employed to evaluate the thermal stability and degradation of the immobilized biocatalysts ...
Lipases from Burkholderia cepacia (Amano PS) and Candida antarctica B (CalB) were immobilized by sol-gel entrapment techniques, using a ternary silane precursors system with vinyl and phenyl non-hydrolyzable groups. The influence of temperature on the catalytic properties of native and immobilized lipases was investigated by incubating the biocatalysts for 24 h at temperatures between 40 C and 80 C, in n-hexane medium. A long term stability study was made with immobilized CaLB biocatalysts by incubation for 120 h in isooctane at 80 C. The acylation of aliphatic sec-alcohols by vinyl acetate was used as model reaction. Thermogravimetric analysis (TGA) was employed to evaluate the thermal stability and degradation of the immobilized biocatalysts ...
Lipase from Burkholderia cepacia (PS) has been immobilized on Accurel MP 1000, and their performance has been compared to that of the most widely used immobilized commercial preparation of this enzyme. The maximum loading was 18 mg protein/g support, and its thermal and solvent stability was much higher than that of the commercial. PS preparations were used in hydrolysis of triacetin and methyl mandelate, in the esterification of oleic acid and ethanol and in the kinetic resolution of 1,3,4-tri-O-benzyl-myo-inositol (DL-1) using vinylacetate as activated acyl donor. For all reactions studied, PS on Accurel was more active than PS-IM. The conversion in the kinetic resolution of racemic DL-1 was optimized using response surface methodology. Optimal conditions were determined to be 2.0 mg/mL of substrate, temperature of 40oC, 2.0 ml of vinyl acetate and without water addition. Under these conditions, maximum loaded Accurel-PS preparation permitted to improve the activity in this kinetic resolution ...
Insight Pharmaceuticals, LLC, has recalled the nasal spray Nostrilla Nasal Decongestant due to potential contamination with Burkholderia cepacia, according to an FDA MedWatch safety alert.
using System.Data.SqlClient; class Program { static void Main() { string connectionString = GetConnectionString(); // Open a sourceConnection to the AdventureWorks database. using (SqlConnection sourceConnection = new SqlConnection(connectionString)) { sourceConnection.Open(); // Perform an initial count on the destination table. SqlCommand commandRowCount = new SqlCommand( "SELECT COUNT(*) FROM " + "dbo.BulkCopyDemoMatchingColumns;", sourceConnection); long countStart = System.Convert.ToInt32( commandRowCount.ExecuteScalar()); Console.WriteLine("Starting row count = {0}", countStart); // Get data from the source table as a SqlDataReader. SqlCommand commandSourceData = new SqlCommand( "SELECT ProductID, Name, " + "ProductNumber " + "FROM Production.Product;", sourceConnection); SqlDataReader reader = commandSourceData.ExecuteReader(); // Open the destination connection. In the real world you would // not use SqlBulkCopy to move data from one table to the other // in the same database. This is ...
Cyclists from Middlesbrough Erimus Rotary Club rode 110 miles to help the Teesside Cystic Fibrosis Centre, based at The James Cook University Hospital
Burkholderia cepacia lipase (BCL) has been proved to be capable of resolution reactions. However, its free form usually exhibits low stability, bad resistance and no reusability, which restrict its further industrial applications. Therefore, it is of great importance to improve the catalytic performance of free lipase in non-aqueous medium. In this work, macroporous resin NKA (MPR-NKA) was utilized as support for lipase immobilization. Racemic transesterification of 1-phenylethanol with vinyl acetate was chosen as model reaction. Compared with its free form, the enzyme activity and enantioselectivity (ees) of the immobilized lipase have been significantly enhanced. The immobilized BCL exhibited a satisfactory thermostability over a wide range of temperature (from 10 to 65°C) and an excellent catalytic efficiency. After being used for more than 30 successive batches, the immobilized lipase still kept most of its activity. In comparison with other immobilized lipases, the immobilized BCL also exhibits
TY - JOUR. T1 - Reclassification of the Specialized Metabolite Producer Pseudomonas mesoacidophila ATCC 31433 as a Member of the Burkholderia cepacia Complex. AU - Loveridge, E Joel. AU - Jones, Cerith. AU - Bull, Matthew J. AU - Moody, Suzy C. AU - Kahl, Małgorzata W. AU - Khan, Zainab. AU - Neilson, Louis. AU - Tomeva, Marina. AU - Adams, Sarah E. AU - Wood, Andrew C. AU - Rodriguez-Martin, Daniel. AU - Pinel, Ingrid. AU - Parkhill, Julian. AU - Mahenthiralingam, Eshwar. AU - Crosby, John. N1 - Copyright © 2017 Loveridge et al.. PY - 2017/7/1. Y1 - 2017/7/1. N2 - Pseudomonas mesoacidophila ATCC 31433 is a Gram-negative bacterium, first isolated from Japanese soil samples, that produces the monobactam isosulfazecin and the β-lactam-potentiating bulgecins. To characterize the biosynthetic potential of P. mesoacidophila ATCC 31433, its complete genome was determined using single-molecule real-time DNA sequence analysis. The 7.8-Mb genome comprised four replicons, three chromosomal (each ...
The species that presently constitute the Burkholderia cepacia complex (Bcc) have multiple roles; they include soil and water saprophytes, bioremediators, and plant, animal and human pathogens. Since the first description of pathogenicity in the Bcc was based on sour skin rot of onion bulbs, this study returned to this plant host to investigate the onion-associated phenotype of the Bcc. Many Bcc isolates, which were previously considered to be non-mucoid, produced copious amounts of exopolysaccharide (EPS) when onion tissue was provided as the sole nutrient. EPS production was not species-specific, was observed in isolates from both clinical and environmental sources, and did not correlate with the ability to cause maceration of onion tissue. Chemical analysis suggested that the onion components responsible for EPS induction were primarily the carbohydrates sucrose, fructose and fructans. Additional sugars were investigated, and all alcohol sugars tested were able to induce EPS production, in ...
The Priebe Laboratory, located in the Enders Research Building of Boston Childrens Hospital, studies the bacterial pathogens Pseudomonas aeruginosa and the Burkholderia cepacia complex, with the long-term goal of developing vaccines and new antimicrobials. Techniques used in Priebes group span multiple fields, including microbiology, molecular biology, genomics, cellular and molecular immunology, and animal models of infection.. ...
Similarity of gene expression patterns in human alveolar macrophages in response to Pseudomonas aeruginosa and Burkholderia cepacia Academic Article ...
As you may know, over the last few months we have been reviewing how cystic fibrosis care is provided for adults living in Dorset. This became necessary because of staffing challenges at Poole Hospitals NHS Foundation Trust (PHFT) which meant that the service there was unsustainable in the long-term.. While this review takes place we have made short-term arrangements so that we can continue to provide care safely. Since February, inpatient care has been provided at University Hospital Southampton NHS Foundation Trust (UHS) while outpatient clinics have continued to be based in Poole but provided by the Southampton team.. Unfortunately from the end of April, PHFT will no longer provide us with the space required to continue offering outpatient clinics in this way. Weve looked at alternative venues but none are able to provide the facilities we need and because of this there is currently no other option but for all outpatient appointments to be held at UHS from the beginning of May. Were still ...
Introduction. The Cystic Fibrosis and Pulmonary Diseases Research and Treatment Center (CF Center) at the University of North Carolina at Chapel Hill traditionally has had a single goal: to "cure" CF lung disease. To this end, we assembled a broad-based biomedical research team to tackle this goal. Thus, we have wide-ranging skills in ion transport physiology (Ussing chamber, patch clamp, confocal surface liquid imaging), mucus cell/mucin secretion biology and biochemistry, transgenic and gene-targeted mouse models, in vivo measures of mucociliary clearance and measures of mucin concentration/hydration, and Phase I clinical trials. In addition, we have added expertise focused on specific CF microbiologic problems of the lung, including Pseudomonas aeruginosa, anaerobic bacteria, and now Burkholderia cepacia. These efforts are complemented by a major effort to identify genetic modifiers of all facets of CF airways pathogenesis. Thus, the airways pathobiology/therapeutics efforts have produced ...
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Gilead Sciences, Inc. today announced that the U.S. Food and Drug Administration (FDA) has granted marketing approval for Cayston(R) (aztreonam for inhalation solution) as a treatment to improve respiratory symptoms in cystic fibrosis (CF) patients with Pseudomonas aeruginosa (P. aeruginosa). Caystons safety and efficacy have not been established in pediatric patients below the age of 7, patients with forced expiratory volume in one second (FEV1) of less than 25 percent or greater than 75 percent predicted, or patients colonized with Burkholderia cepacia.. Cayston is administered at a dose of 75 mg three times daily over a 28-day period and is delivered via the Altera(R) Nebulizer System, a portable, drug-specific delivery device using the eFlow(R) Technology Platform, developed by PARI Pharma GmbH. PARI Pharma also contributed to the development of Caystons drug formulation for delivery with the Altera Nebulizer System. Cayston will be available in the United States by the end of next week ...
As Western North Carolinas Cystic Fibrosis center, we are here to provide care to children from birth to 18 years. Visit one of our locations to learn more
Today we went to the Cystic Fibrosis Center for Laineys 3rd visit. Since this time last month Lainey has grown 1/2 inch and gained 1 1/2 pounds. She is now 23 1/2 inches long and 10 lbs. 15.9 oz! Still not the 3 lbs. they had wanted to see (she is still in the 10th percentile for weight), but we are working on that. Due to the frequency and consistancy of her bowel movements, it has been concluded that changes with her enzymes need to be made if we wish to see an increase in weight. To assist in weight gain, Dr. Caplan decided to try upping her enzymes to 2 per meal and taken in applesauce. Originally we tried the applesauce, but it was way too difficult to feed it to a newborn. Now she is loving the addition of applesauce and doesnt seem to mind the new requirement! The thoughts behind the applesauce are that the enzymes will make it to the small intestine before the formula. This is important because the enzymes aid in digestion, fat and vitiamin absorption, and in the end ~ weight gain. If ...
The KOMP Repository Collection is located at the MMRRC at the University of California, Davis and Childrens Hospital Oakland Research Institute. Question? Comments? For Mice, Cells, and germplasm please contact us at [email protected], US 1-888-KOMP-MICE or International +1-530-752-KOMP, or for vectors [email protected] or +1-510-450-7917 ...
ID CP000871; SV 1; circular; genomic DNA; STD; PRO; 167422 BP. XX AC CP000871; AAVB01000000-AAVB01000037; XX PR Project:PRJNA17407; XX DT 16-NOV-2007 (Rel. 93, Created) DT 12-DEC-2013 (Rel. 119, Last updated, Version 2) XX DE Burkholderia multivorans ATCC 17616 plasmid pBMUL01, complete sequence. XX KW . XX OS Burkholderia multivorans ATCC 17616 OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; OC Burkholderiaceae; Burkholderia; Burkholderia cepacia complex. OG Plasmid pBMUL01 XX RN [1] RP 1-167422 RG US DOE Joint Genome Institute RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., RA Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., RA Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Tiedje J., RA Richardson P.; RT "Complete sequence of plasmid1 of Burkholderia multivorans ATCC 17616"; RL Unpublished. XX RN [2] RP 1-167422 RG US DOE Joint Genome Institute RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., ...
Members of the Burkholderia cepacia complex (Bcc), such as B. ambifaria, are effective biocontrol strains, for instance, as plant growth-promoting bacteria; however, Bcc isolates can also cause severe respiratory infections in people suffering from cystic fibrosis (CF). No distinction is known between isolates from environmental and human origins, suggesting that the natural environment is a potential source of infectious Bcc species. While investigating the presence and role of phase variation in B. ambifaria HSJ1, an isolate recovered from a CF patient, we identified stable variants that arose spontaneously irrespective of the culture conditions. Phenotypic and proteomic approaches revealed that the transition from wild-type to variant types affects the expression of several putative virulence factors. By using four different infection models (Drosophila melanogaster, Galleria mellonella, macrophages and Dictyostelium discoideum), we showed that the wild-type was more virulent than the ...
A polyphasic taxonomic study including DNA-DNA reassociation experiments and an extensive biochemical characterization was performed on 14 Burkholderia isolates from moss gametophytes of nutrient-poor plant communities on the southern Baltic Sea coast in northern Germany. The strains were classified within two novel species, for which the names Burkholderia bryophila sp. nov. and Burkholderia megapolitana sp. nov. are proposed. The former species also includes isolates from grassland and agricultural soil collected in previous studies. Strains Burkholderia bryophila 1S18T (=LMG 23644T =CCUG 52993T) and Burkholderia megapolitana A3T (=LMG 23650T =CCUG 53006T) are the proposed type strains. They were isolated from Sphagnum rubellum and Aulacomnium palustre, respectively, growing in the Ribnitzer Großes Moor nature reserve (Mecklenburg-Pommern, Germany). All moss isolates of both novel species showed antifungal activity against phytopathogens as well as plant-growth-promoting properties.
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
What led me, initially, to consider a career in the medical field?. When I was only 6 years old my brother Emmanuel, who was sick w/ Cystic Fibrosis and was hospitalized a lot, died at the age of 3. I was young, but remember his frequent hospitalizations, and I was always intrigued about the circumstances---. When I was 14, another brother, Patrick, was born and diagnosed w/ CF. At the time, my parents were told he would most likely never live to leave the hospital (at 6 weeks old) nor at least grow much past toddler-hood. Over the years, I watched my mother, who was not a trained nurse, give him care at home, from nebulizer treatments to frequent manual CPT.. Patrick lived to be nearly 41 years old, and worked as a licensed Physician Assistant in Hematology/Oncology until his illness forced his retirement.. If I have to give a one sentence answer; I watched and observed a lot of "medicine" in my growing up years, and was intrigued by it all-how better to get involved than to become a ...
Achieving Nutrition Needs. Calories - People with CF have calorie requirements that are higher than people without CF. They need to consume up to twice as many calories daily Protein - People with CF need 1.5 to 2 times more protein in their diets. Fat - Due to poor fat absorption, people with CF need 10% more fat than the average person. Carbohydrates - Carbs are a great source of energy and calories and do not need to be avoided, even in the setting of diabetes. Salt and chloride (Table salt) - All people with CF need additional salt in their diet to replace higher than normal salt losses.. Vitamin and mineral supplementation - People with CF and pancreatic insufficiency have difficulty absorbing fat soluble vitamins A, D, E and K. CF specific vitamins were designed to offer higher levels of vitamins and minerals that CF patients need. Enzyme replacement therapy. ...
For chronically ill patients, patient assistance offers peace of mind that helps patients focus on health and healing. To raise funds for patients with cystic fibrosis and collect food for people in need, the UNC Adult Cystic Fibrosis Center will host the second annual fall fundraiser and cornhole tournament this Sunday, from 12 - 4 pm, at 400 Market Street in Southern Village in Chapel Hill.
All Childrens Hospital (ACH) is a Cystic Fibrosis Foundation accredited care Center. Caring for more than 170 pediatric patients the Cystic Fibrosis Center provides comprehensive services, ranging from evaluation and diagnosis to treatment and education.
Dans le cadre dun doctorat en art et sciences de lart, je mintéresse plus particulièrement à la problématique de la restauration de peintures contemporaines comportant du bleu de phtalocyanine. Sa forme alpha non stabilisée est sujette à la floculation et à la cristallisation, ce qui peut entraîner des modifications optiques indésirables à savoir une perte de pouvoir couvrant et un glissement de teinte vers le vert. Ces phénomènes sobservent plus particulièrement en présence dhydrocarbures aromatiques. Or, un certain nombre de produits utilisés en restauration contiennent ce type de solvant.
The major part of this thesis describes the asymmetric synthesis of β- and γ-amino alcohols through the combination of ruthenium catalyzed racemization and enzymatic kinetic resolution.. The dynamic kinetic resolution, DKR, protocol for chlorohydrins was improved by employing Bäckvalls catalyst, which is a base activated racemization catalyst, in combination with Burkholderia cepacia lipase. These optimized conditions broadened the substrate scope and improved the yields and ees of the obtained chlorohydrin acetates. The utility of the method was demonstrated in the synthesis of (S)-salbutamol.. In the second part of the thesis, DKR was utilized in the enantio-determining step of the total synthesis of (R)-duloxetine. Optimized DKR conditions, combining Bäckvalls catalyst together with Candida antarctica lipase B, afforded a β-cyano acetate in high yield and ee. (R)-Duloxetine was accessible through synthetic alterations of the enantioenriched β-cyano acetate in high overall ...
DNA mismatch repair (MMR) is a highly conserved biological pathway that plays a key role in maintaining genomic stability. MMR corrects DNA mismatches generated during DNA replication, thereby preventing mutations from becoming permanent in dividing cells. MMR also suppresses homologous recombination and was recently shown to play a role in DNA damage signaling. Defects in MMR are associated with genome-wide instability, predisposition to certain types of cancer including HNPCC, resistance to certain chemotherapeutic agents, and abnormalities in meiosis and sterility in mammalian systems. The Escherichia coli MMR pathway has been extensively studied and is well characterized. In E. coli, the mismatch-activated MutS-MutL-ATP complex licenses MutH to incise the nearest unmethylated GATC sequence. UvrD and an exonuclease generate a gap. This gap is filled by pol III and DNA ligase. The GATC sites are then methylated by Dam. Several human MMR proteins have been identified based on their homology to ...
The bacterium Burkholderia multivorans evolves and adapts in bursts to survive in the lungs of cystic fibrosis patients, according to a study published in...
Kleen Machine Flex Wipes Disinfectant Wipes. Case of 2 buckets with 800 8" x 6" towelettes per bucket. Flex Wipes disinfectant wipes are a one step cleaner, disinfectant and deodorizer. The antibacterial formula starts killing bacteria on contact.. FLEX WIPES Disinfectant Wipes are effective. against:. ****Bacteria. Staphylococcus aureus - Community Associated Methicillin-Resistant. [CA-MRSA] [NRS384] [USA300]. Staphylococcus aureus - Community Associated Methicillin-Resistant. [CA-MRSA] [NRS123] [USA400]. Staphylococcus aureus (Staph). Salmonella enterica (Salmonella). Enterococcus faecalis- Vancomycin Resistant [VRE]. Escherichia coli [E. coli]. ESBL Escherichia coli - [Extended spectrum beta-lactamase producing. E. coli]. Klebsiella pneumoniae [Klebsiella]. Legionella pneumophilia. Burkholderia cepacia. Salmonella schottmuelleri [Salmonella]. Salmonella typhi [Salmonella]. Serratia marcescens [Serratia]. Shigella dysenteriae [Shigella]. Staphylococcus aureus-Vancomycin Intermediate Resistant ...
Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides.
Welcome to the Cystic Fibrosis Mutation Database (CFTR1), devoted to the collection of mutations in the CFTR gene for the international cystic fibrosis genetics research community. It was initiated by the Cystic Fibrosis Genetic Analysis Consortium in 1989 to increase and facilitate communications among CF researchers, and is maintained by the Cystic Fibrosis Centre at the Hospital for Sick Children in Toronto. The specific aim of the database is to provide up to date information about individual mutations in the CFTR gene. In a major upgrade in 2010, all known CFTR mutations and sequence variants have been converted to the standard nomenclature recommended by the Human Genome Variation Society. In addition, an on-line process for the submission of new mutations has been added. While we will continue to ensure the quality of the data, we urge the international community to give us feedback and suggestions. Please send email to cftr.admin ...
Various methods, including OGTT, have been used at different cystic fibrosis centres for the screening and diagnosis of CFRD. At the authors unit patients with cystic fibrosis have been screened for diabetes at least annually using RBG and HbA1c for some years. In this study we found that an abnormal RBG (,11.0 mmol/l), when used alone, has a poor sensitivity in the diagnosis of CFRD. In common with the study by Lanng and colleagues,5 we also found that an abnormal FBG and the presence of symptoms of hyperglycaemia when used alone have poor sensitivity in the diagnosis of CFRD. Lanng and colleagues reported that only 16% of their patients with OGTT defined diabetes had abnormal HbA1c. This is in contrast to 83% (95% CI 62 to 100) of our diabetic patients with abnormal HbA1c values. In the Danish study, ion exchange chromatography was used to measure HbA1c and a higher reference range was used (upper limit 6.4%). These differences are insufficient to explain the large differences in the ...
Please Note: Ortholuge cannot be used for certain RBB analyses involving closely-related strains (e.g. Burkholderia cenocepacia J2315 versus Burkholderia cenocepacia AU 1054) because the phylogenetic distance between each ingroup protein is very small in relation to the phylogenetic distance between each ingroup protein and the associated outgroup protein. In these cases, the "Ortholuge Classification" value will be left blank which indicates Ortholuge analysis was not peformed, however the two proteins may very well be orthologs. In other cases, only some rows will be missing Ortholuge classifications, which can occur when a suitable gene could not be found in the outgroup species ...
Its hard to put into words how I feel toward my Heavenly Father and the Savior. I have so many reasons to be thankful for what I have gone through & endured. Because of my trials in life I have gained a strong testimony of Faith. I have always had Faith in the Lord and in Prayer. As I faced the decision to get a double lung transplant I had to rely on faith to make the choice.....By listening to the spirit, I was guided to Pittsburgh where my life was saved. I believe prayers are answered. I have always tried to follow the philosophy of this: If I do what the Lord expects of me, he will give me what I want & need. That has always worked for me. I Believe in MIRACLES..........My life on this earth is a miracle- I prayed on my knees for years to be healed from my lung disease. I knew that through a priesthood blessing (if it was Gods will) I could be healed. So many times I thought he would heal me instantly...my faith was tested as I continued to get sick and no cure was in my future. I turned ...
Its hard to put into words how I feel toward my Heavenly Father and the Savior. I have so many reasons to be thankful for what I have gone through & endured. Because of my trials in life I have gained a strong testimony of Faith. I have always had Faith in the Lord and in Prayer. As I faced the decision to get a double lung transplant I had to rely on faith to make the choice.....By listening to the spirit, I was guided to Pittsburgh where my life was saved. I believe prayers are answered. I have always tried to follow the philosophy of this: If I do what the Lord expects of me, he will give me what I want & need. That has always worked for me. I Believe in MIRACLES..........My life on this earth is a miracle- I prayed on my knees for years to be healed from my lung disease. I knew that through a priesthood blessing (if it was Gods will) I could be healed. So many times I thought he would heal me instantly...my faith was tested as I continued to get sick and no cure was in my future. I turned ...
Temocillin. Molecular model of the drug temocillin (C16.H18.N2.O7.S2), a beta-lactamase-resistant penicillin used to treat multiresistant Gram-negative bacterial infections. Atoms are represented as spheres and are colour-coded: carbon (grey), hydrogen (white), nitrogen (blue), oxygen (red) and sulphur (yellow). Illustration. - Stock Image F017/0687
Gardening question - Gladioli question: My gladiolis havent flowered this year. Should i leave bulbs in or remove them? What should i do with the leaves? Still new to
Biotin (vitamin H or vitamin B7) is the essential cofactor of biotin-dependent carboxylases, such as pyruvate carboxylase and acetyl-CoA carboxylase. Mammals cannot synthesize biotin, while in bacteria, fungi, and plants it is synthesized from pimelate thioester through different pathways. In E. coli and many organisms, pimelate thioester is derived from malonyl-ACP. The pathway starts with the methylation to malonyl-ACP methyl ester, followed by the fatty acid chain elongation cycle to form pimeloyl-ACP methyl ester, which is then demethylated to form pimeloyl-ACP [MD:M00572]. Pimeloyl-ACP is converted to biotin through the final four steps in the biotin bicyclic ring assembly, which are conserved among biotin-producing organisms [MD:M00123]. In B. subtilis, biotin is derived from pimeloyl-ACP formed by oxidative cleavage of long-chain acyl-ACPs [MD:M00573]. Some bacteria synthesize biotin from pimeloyl-CoA derived from pimelate [MD:M00577]. Biotin is covalently attached to biotin-dependent ...
Two Burkholderia strains 8111 and 8201 were isolated from root nodules of Dalbergia odorifera, an endemic woody legume in southern China. Phylogenetic analysis of 16S rRNA gene and 16S-23S gene intergenic spacer (ITS) showed that these two bacterial strains were closely related to Burkholderia cepacia and they were also similar in carbon source utilization using Biolog GN2 plate tests. The DNA G+C content of strains 8111 and 8201 were 65.8 and 65.5 mol%. Inoculation tests demonstrated that both strains 8111 and 8201 formed functional root nodules in their original host D. odorifera, and significantly enhanced plant growth (as measured by plant biomass and nitrogen content), compared to the no-inoculated control plants. Sequencing of 16S rRNA gene in nodules of D. odorifera seedlings inoculated with strains 8111 and 8201 confirmed their identity. However, these two strains did not induce root nodulation in Acacia auriculiformis and Erythrophleum fordii. This implies that the nodulation capacity between
Cystic fibrosis (CF) is a genetic disease that causes build-up of thick, sticky mucus in the lungs, pancreas and other organs. As most patients with CF experience pancreatic insufficiency and some patients have CF-related liver disease, constipation, failure to thrive, abdominal pain, and distal intestinal obstruction syndrome, they require multidisciplinary care.. To help these patients acheive the best possible outcomes, Mary Abigail Garcia, M.D., (gastroenterologist) and the CF pulmonary care team developed the Cystic Fibrosis/Gastroenterology Clinic, which combines the care provided by the Cystic Fibrosis Center and our Gastroenterology division. The Cystic Fibrosis Center is a CF Foundation-accredited center, which sees children from southern California, Arizona and Mexico.. Along with gastroenterology and CF pulmonary specialists, the Cystic Fibrosis/Gastroenterology Clinic includes nutrition, social work and nursing.. The clinic is held every 3rd Thursday morning of the month. For more ...
TY - JOUR. T1 - OXA-46, a new class D β-lactamase of narrow substrate specificity encoded by a blaVIM-1-containing integron from a Pseudomonas aeruginosa clinical isolate. AU - Giuliani, Francesco. AU - Docquier, Jean Denis. AU - Riccio, Maria Letizia. AU - Pagani, Laura. AU - Rossolini, Gian Maria. PY - 2005/5. Y1 - 2005/5. N2 - A novel OXA-type enzyme, named OXA-46, was found to be encoded by a gene cassette inserted into a class 1 integron from a multidrug-resistant Pseudomonas aeruginosa clinical isolate. The variable region of the integron also contained a blaVIM-1 metallo-β-lactamase cassette and a duplicated aacA4 aminoglycoside acetyltransferase cassette. OXA-46 belongs to the OXA-2 lineage of class D β-lactamases. It exhibits 78% sequence identity with OXA-2 and the highest similarity (around 92% identity) with another OXA-type enzyme detected in clinical isolates of Burkholderia cepacia and in unidentified bacteria from a wastewater plant. Expression of blaOXA-46 in Escherichia coli ...
TY - JOUR. T1 - Burkholderia pseudomallei Antibodies in Individuals Living in Endemic Regions in Northeastern Brazil. AU - Rolim, D.B.. AU - Vilar, D.C.F.L.. AU - De Goes Cavalcanti, L.P.. AU - Freitas, C.L.B.N.. AU - Inglis, Tim. AU - Nobre Rodrigues, J.L.N.. AU - Nagao-Dias, A.T.. PY - 2011. Y1 - 2011. N2 - A seroepidemiological investigation was conducted among the population of two municipalities in Northeastern Brazil. Immunoglobulin M (IgM) and IgG antibodies to Burkholderia pseudomallei were positive in 51.27% (161 in 317 samples) and 58.49% (186), respectively. IgM titers were higher in children than in adults. On the contrary, IgG increased progressively with age. We observed a significant association between agricultural occupation and raised IgM titers (P ,0.005) and IgG titers (P ,0.001), and between construction workers and raised IgG titers (P = 0.005). Antibody IgG avidities did not correlate with age. The highest titers of antibodies (1/800) showed the highest antibody avidity ...

Taxonomy and Identification of the Burkholderia cepacia Complex | Journal of Clinical MicrobiologyTaxonomy and Identification of the Burkholderia cepacia Complex | Journal of Clinical Microbiology

Burkholderia mallei, Burkholderia pseudomallei,Burkholderia gladioli, Burkholderia plantarii,Burkholderia glumae, Burkholderia ... Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, and Burkholderia cepacia genomovars I and III. J. ... Burkholderia ubonensis, Burkholderia caledonica, Burkholderia fungorum,Burkholderia stabilis, and Burkholderia ambifaria(1, 10 ... 2001) Burkholderia cepacia genomovar VI, a new member of the Burkholderia cepacia complex isolated from cystic fibrosis ...
more infohttps://jcm.asm.org/content/39/10/3427?ijkey=016117cc34c5b762756dd57266ee67d3be91427d&keytype2=tf_ipsecsha

Social interactions in the Burkholderia cepacia complex: biofilms and quorum sensing. - Semantic ScholarSocial interactions in the Burkholderia cepacia complex: biofilms and quorum sensing. - Semantic Scholar

... cepacia complex cells and the role of quorum sensing in B. cepacia complex biofilm formation. ... There is convincing evidence that B. cepacia complex bacteria can form biofilms, not only on abiotic surfaces (e.g., glass and ... This article presents an overview of the molecular mechanisms involved in B. cepacia complex biofilm formation, the increased ... Burkholderia cepacia complex bacteria are opportunistic pathogens that cause respiratory tract infections in susceptible ...
more infohttps://www.semanticscholar.org/paper/Social-interactions-in-the-Burkholderia-cepacia-co-Coenye/a43dd7fe5d1840c2cf96112de121d185a59299c6

Scientific Articles - The International Burkholderia cepacia Working GroupScientific Articles - The International Burkholderia cepacia Working Group

The International Burkholderia cepacia Working Group Fostering research in Burkholderia cepacia complex ... Burkholderia cepacia Workgroup. 2018. Multistate Outbreak of Burkholderia cepacia Complex Bloodstream Infections After Exposure ... multireplicon Burkholderia cepacia complex. Nat Rev 3:144-156.. Valvano MA. 2015. Intracellular survival of Burkholderia ... cepacia Investigation Workgroup. 2018. A multistate investigation of health care-associated Burkholderia cepacia complex ...
more infohttps://ibcwg.org/index.php/home/publications/

Burkholderia cepacia in Healthcare Settings 
 | HAI | CDCBurkholderia cepacia in Healthcare Settings | HAI | CDC

General Information about B. cepacia. Burkholderia cepacia [burk-hōld-er-ee-uh si-pay-shee-uh] (also called B. cepacia) is the ... B cepacia is a known cause of infections in hospitalized patients.. Symptoms of B. cepacia infection. The effects of B. cepacia ... B. cepacia bacteria are often resistant to common antibiotics.. Populations susceptible to B. cepacia infection. B. cepacia ... Exposure to B. cepacia in the environment.. Treatment of B. cepacia infection. B. cepacia can be resistant to many common ...
more infohttps://www.cdc.gov/hai/organisms/bcepacia.html

Burkholderia cepacia Related ArticlesBurkholderia cepacia Related Articles

Control of Cotton Seedling Damping-off in the Field by Burkholderia (Pseudomonas) cepacia. K. Zaki, I. J. Misaghi, and A. ... Related articles on Burkholderia cepacia from APS Journals. Mobilization, Cloning, and Sequence Determination of a Plasmid- ... Encoded Polygalacturonase from a Phytopathogenic Burkholderia (Pseudomonas) cepacia. Carlos F. Gonzalez, Elizabeth A. Pettit, ...
more infohttps://www.apsnet.org/publications/apsnetfeatures/Pages/BcepaciaRelatedArticles.aspx

The multifarious, multireplicon Burkholderia cepacia complex.  - PubMed - NCBIThe multifarious, multireplicon Burkholderia cepacia complex. - PubMed - NCBI

The multifarious, multireplicon Burkholderia cepacia complex.. Mahenthiralingam E1, Urban TA, Goldberg JB. ... The Burkholderia cepacia complex (Bcc) is a collection of genetically distinct but phenotypically similar bacteria that are ... cepacia syndrome, which is characterized by a rapid decline in lung function that can include invasive disease. Here we ... Burkholderia cepacia complex/genetics*. *Burkholderia cepacia complex/pathogenicity*. *Burkholderia cepacia complex/physiology ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/15643431?dopt=Abstract

Burkholderia cepacia: Friend or Foe?Burkholderia cepacia: Friend or Foe?

Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis and Burkholderia cepacia genomovars I and III. J ... Burkholderia cepacia: Friend or Foe?. Parke, J.L. 2000. Burkholderia cepacia: Friend or Foe? The Plant Health Instructor. DOI: ... Burkholderia cepacia genomovar VI, a new member of the Burkholderia cepacia complex isolated from cystic fibrosis patients. Int ... Proposal to accommodate Burkholderia cepacia genomovar VI as Burkholderia dolosa sp. nov. Int J Syst Evol Microbiol 54:689-691. ...
more infohttp://www.apsnet.org/publications/apsnetfeatures/Pages/BurkholderiaCepacia.aspx

Burkholderia cepacia complex - microbewikiBurkholderia cepacia complex - microbewiki

... multireplicon Burkholderia cepacia complex." Nature. 3 Hua, C.N.C., Tokeshi, J. "Emergence of Burkholderia cepacia in Honolulu ... 6 "Burkholderia cepacia in Healthcare Settings." Centers for Disease Control and Prevention. 7 Gilchrist, F.J., Webb, A.K., ... This is often referred to as the Burkholderia cepacia complex (BCC) [1][2]. It was originally recognized by W. H. Burkholder in ... Burkholderia cepacia complex. From MicrobeWiki, the student-edited microbiology resource. Revision as of 21:03, 10 February ...
more infohttps://microbewiki.kenyon.edu/index.php?title=Burkholderia_cepacia_complex&oldid=119595

Interspecies communication between Burkholderia cepacia and Pseudomonas aeruginosa.  - PubMed - NCBIInterspecies communication between Burkholderia cepacia and Pseudomonas aeruginosa. - PubMed - NCBI

Interspecies communication between Burkholderia cepacia and Pseudomonas aeruginosa.. Lewenza S1, Visser MB, Sokol PA. ... Burkholderia cepacia and Pseudomonas aeruginosa are opportunistic pathogens that commonly cause pulmonary infections in cystic ... These studies suggest that B. cepacia CepR can use N-acyl-HSLs synthesized by RhlI and LasI and that P. aeruginosa LasR and ... It is possible that a mixed bacterial population of B. cepacia and P. aeruginosa can coordinately regulate some of their ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/12381027

Burkholderia cepacia complex - WikipediaBurkholderia cepacia complex - Wikipedia

Burkholderia cepacia complex (BCC), or simply Burkholderia cepacia, is a group of catalase-producing, lactose-nonfermenting, ... "Burkholderia cepacia complex". NCBI Taxonomy Browser. 87882. Type strain of Burkholderia cepacia complex at BacDive - the ... UMR: Burkholderia cepacia CFF: Research Pathema-Burkholderia Resource Loutet SA, Valvano MA (October 2010). "A decade of ... The organism is usually cultured in Burkholderia cepacia agar (BC agar) which contains crystal violet and bile salts to inhibit ...
more infohttps://en.wikipedia.org/wiki/Burkholderia_cepacia_complex

Burkholderia cepacia complex Cervical Osteomyelitis in an Intravenous Drug UserBurkholderia cepacia complex Cervical Osteomyelitis in an Intravenous Drug User

... we present a case of Burkholderia cepacia complex cervical osteomyelitis in an intravenous drug user. Burkholderia cepacia ... Burkholderia cepacia complex Cervical Osteomyelitis in an Intravenous Drug User. Sui Kwong Li1 and William B. Messer1,2 ... Burkholderia cepacia complex are closely related aerobic Gram-negative bacilli that are well described in infections among ... Burkholderia cepacia complex vertebral osteomyelitis is an infrequently described clinical syndrome in the literature though it ...
more infohttps://www.hindawi.com/journals/criid/2018/7638639/

Burkholderia cepacia (Palleroni and Holmes) Yabuuchi et al. ATCC ®Burkholderia cepacia (Palleroni and Holmes) Yabuuchi et al. ATCC ®

Genomic DNA from Burkholderia cepacia strain 249 TypeStrain=False Application: ... Genomic DNA from Burkholderia cepacia strain 249 [ATCC® 17616™] Biosafety Level 1 Biosafety classification is based on U.S. ... Burkholderia cepacia (Palleroni and Holmes) Yabuuchi et al. (ATCC® 17616D-5™) Strain Designations: Genomic DNA from ... Nucleotide (GenBank) : AY741333 Burkholderia cepacia strain ATCC 17616 16S ribosomal RNA gene, partial sequence ...
more infohttps://www.atcc.org/Products/All/17616D-5.aspx?p=1&rel=%7B0%7D

Burkholderia cepacia (Palleroni and Holmes) Yabuuchi et al. ATCC ®Burkholderia cepacia (Palleroni and Holmes) Yabuuchi et al. ATCC ®

Genomic DNA from Burkholderia cepacia strain 249 TypeStrain=False Application: ... Burkholderia cepacia (Palleroni and Holmes) Yabuuchi et al. ATCC® 17616D-5™ dried Total DNA: At least 5 µg in 1X TE buffer, ... Genomic DNA from Burkholderia cepacia strain 249 [ATCC® 17616™] Biosafety Level 1 Biosafety classification is based on U.S. ... Burkholderia cepacia (Palleroni and Holmes) Yabuuchi et al. (ATCC® 17616D-5™) Strain Designations: Genomic DNA from ...
more infohttps://www.atcc.org/Global/Products/B/8/9/4/17616D-5.aspx

British Library EThOS: Antimicrobial strategies against Burkholderia cepaciaBritish Library EThOS: Antimicrobial strategies against Burkholderia cepacia

Human (hBD-1) and murine (mBD-1) b-defensins were examined for activity against B. cepacia. In contrast to the salt-sensitive ... The susceptibility of the B. cepacia strain panel to plant extracts was investigated, in particular the activity of aqueous ... The project focused on a panel of 20 strains of B. cepacia complex. Isolates included strains representing major epidemic ... In contrast, two hours incubation with AGE produced morphological changes in the cellular structure of B. cepacia consistent ...
more infohttp://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.657471

Burkholderia cepacia | definition of Burkholderia cepacia by Medical dictionaryBurkholderia cepacia | definition of Burkholderia cepacia by Medical dictionary

What is Burkholderia cepacia? Meaning of Burkholderia cepacia medical term. What does Burkholderia cepacia mean? ... Looking for online definition of Burkholderia cepacia in the Medical Dictionary? Burkholderia cepacia explanation free. ... Burkholderia cepacia. Burkholderia cepacia. a bacterial species found in rotted onions and in clinical specimens; commonly ... Synonym(s): Pseudomonas cepacia. Burkholderia cepacia. formerly Pseudomonas cepacia. A group of bacteria found in the ...
more infohttps://medical-dictionary.thefreedictionary.com/Burkholderia+cepacia

Federal Register
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      Allethrin, Bendiocarb, Burkholderia cepacia, Fenridazon potassium, and Molinate; Tolerance...Federal Register :: Allethrin, Bendiocarb, Burkholderia cepacia, Fenridazon potassium, and Molinate; Tolerance...

... and biological pesticide Burkholderia cepacia because EPA canceled food registrations or deleted food uses from registrations ... 3. Burkholderia cepacia type Wisconsin. On August 28, 2002 (67 FR 55236) (FRL-7189-4), EPA published a notice in the Federal ... Allethrin, Bendiocarb, Burkholderia cepacia, Fenridazon potassium, and Molinate; Tolerance Actions. A Rule by the Environmental ... EPA is revoking the tolerance exemption in 40 CFR 180.1115 for residues of Burkholderia cepacia type Wisconsin in or on all raw ...
more infohttps://www.federalregister.gov/documents/2004/09/29/04-21695/allethrin-bendiocarb-burkholderia-cepacia-fenridazon-potassium-and-molinate-tolerance-actions

https://www.thefreelibrary.com/Environmental+Burkholderia+cepacia+complex+isolates+in+human...-a0161011310https://www.thefreelibrary.com/Environmental+Burkholderia+cepacia+complex+isolates+in+human...-a0161011310

Environmental Burkholderia cepacia complex isolates in human infections.(DISPATCHES) by Emerging Infectious Diseases; Health ... Identification and population structure of Burkholderia stabilis sp. nov. (formerly Burkholderia cepacia genomovar IV). J Clin ... cepacia+complex+isolates+in+human...-a0161011310. *APA style: Environmental Burkholderia cepacia complex isolates in human ... Commercial use of Burkholderia cepacia. Emerg Infect Dis. 1999;5:305-6. (9.) Govan JRW, Balendreau J, Vandamme P. Burkholderia ...
more infohttps://www.thefreelibrary.com/Environmental+Burkholderia+cepacia+complex+isolates+in+human...-a0161011310

Multistate Outbreak of Burkholderia cepacia Infections Associated with Oral Liquid Docusate Sodium 
 | HAI | CDCMultistate Outbreak of Burkholderia cepacia Infections Associated with Oral Liquid Docusate Sodium | HAI | CDC

For additional information on B. cepacia please visit: Burkholderia cepacia in Healthcare Settings. ... FDA Alert August 8, 2017: FDA updates on 2017 Burkholderia cepacia contaminationexternal icon ... Multistate Outbreak of Burkholderia cepacia Infections Associated with Oral Liquid Docusate Sodium. ... Facilities that experience B. cepacia complex infections among non-cystic fibrosis patients or clusters of B. cepacia complex ...
more infohttp://www.cdc.gov/hai/outbreaks/b-cepacia/index.html

Suppurative Mediastinitis Secondary to Burkholderia Cepacia in a Patient with Cystic FibrosisSuppurative Mediastinitis Secondary to Burkholderia Cepacia in a Patient with Cystic Fibrosis

... Ronald B George,1 Yannick ... "Suppurative Mediastinitis Secondary to Burkholderia Cepacia in a Patient with Cystic Fibrosis," Canadian Respiratory Journal, ...
more infohttps://www.hindawi.com/journals/crj/2006/495720/cta/

hdl IVa - (S)-2-haloacid dehalogenase 4A - Burkholderia cepacia - hdl IVa gene & proteinhdl IVa - (S)-2-haloacid dehalogenase 4A - Burkholderia cepacia - hdl IVa gene & protein

Burkholderia cepacia (Pseudomonas cepacia). 231. UniRef90_Q51645. Cluster: (S)-2-haloacid dehalogenase 4A. 3. ... Burkholderia cepacia (Pseudomonas cepacia). Paraburkholderia oxyphila. 231. UniRef50_Q51645. Cluster: (S)-2-haloacid ... Burkholderia cepacia (Pseudomonas cepacia). ,p>This subsection of the ,a href="http://www.uniprot.org/help/names_and_taxonomy_ ... sp,Q51645,HAD4_BURCE (S)-2-haloacid dehalogenase 4A OS=Burkholderia cepacia OX=292 GN=hdl IVa PE=1 SV=3 ...
more infohttp://www.uniprot.org/uniprot/Q51645

Quorum Sensing in Burkholderia cepacia: Identification of the LuxRI Homologs CepRI | Journal of BacteriologyQuorum Sensing in Burkholderia cepacia: Identification of the LuxRI Homologs CepRI | Journal of Bacteriology

Burkholderia cepacia (previously Pseudomonas cepacia) is an important pathogen in patients with cystic fibrosis (23). Twenty ... 1997) Occurrence of multiple genomovars of Burkholderia cepacia in cystic fibrosis patients and proposal of Burkholderia ... Quorum Sensing in Burkholderia cepacia: Identification of the LuxRI Homologs CepRI. Shawn Lewenza, Barbara Conway, E. P. ... Quorum Sensing in Burkholderia cepacia: Identification of the LuxRI Homologs CepRI. Shawn Lewenza, Barbara Conway, E. P. ...
more infohttps://jb.asm.org/content/181/3/748?ijkey=acbce1282893380302f31794714ff1d2f484de24&keytype2=tf_ipsecsha

Quorum Sensing in Burkholderia cepacia: Identification of the LuxRI Homologs CepRI | Journal of BacteriologyQuorum Sensing in Burkholderia cepacia: Identification of the LuxRI Homologs CepRI | Journal of Bacteriology

Burkholderia cepacia (previously Pseudomonas cepacia) is an important pathogen in patients with cystic fibrosis (23). Twenty ... 1997) Occurrence of multiple genomovars of Burkholderia cepacia in cystic fibrosis patients and proposal of Burkholderia ... Quorum Sensing in Burkholderia cepacia: Identification of the LuxRI Homologs CepRI. Shawn Lewenza, Barbara Conway, E. P. ... Quorum Sensing in Burkholderia cepacia: Identification of the LuxRI Homologs CepRI. Shawn Lewenza, Barbara Conway, E. P. ...
more infohttps://jb.asm.org/content/181/3/748

Azithromycin in Patients With CF, Infected With Burkholderia Cepacia Complex - Full Text View - ClinicalTrials.govAzithromycin in Patients With CF, Infected With Burkholderia Cepacia Complex - Full Text View - ClinicalTrials.gov

Pulmonary infection with Burkholderia cepacia complex (BCC) in patients with CF is often associated with a more rapid decline ... Azithromycin in Patients With CF, Infected With Burkholderia Cepacia Complex. The recruitment status of this study is unknown. ...
more infohttps://clinicaltrials.gov/ct2/show/NCT00298922

Pathogenicity, virulence factors, and strategies to fight against Burkholderia cepacia complex pathogens and related species |...Pathogenicity, virulence factors, and strategies to fight against Burkholderia cepacia complex pathogens and related species |...

The Burkholderia cepaciacomplex (Bcc) is a group of 17 closely related species of the β-proteobacteria subdivision that emerged ... nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia ... Saldías MS, Valvano MA (2009) Interactions of Burkholderia cenocepacia and other Burkholderia cepacia complex bacteria with ... a complex within the Burkholderia cepacia complex comprises at least two novel species: Burkholderia contaminans sp. nov. and ...
more infohttps://link.springer.com/article/10.1007%2Fs00253-010-2528-0

An epidemic of burkholderia cepacia transmitted between patients with and without cystic fibrosis. | Riley LabAn epidemic of burkholderia cepacia transmitted between patients with and without cystic fibrosis. | Riley Lab

An epidemic of burkholderia cepacia transmitted between patients with and without cystic fibrosis.. Authors. Holmes A, Nolan R ... Burkholderia cepacia is an important pathogen in cystic fibrosis (CF) and an infrequent cause of nosocomial infection in non-CF ...
more infohttp://www.bio.umass.edu/biology/riley/node/169
  • The first detailed description of the clinical significance of " B. cepacia " colonization and infection was published in 1984 ( 47 ). (asm.org)
  • documented the increasing prevalence of " B. cepacia " colonization and infection in the Toronto, Canada, CF treatment center and described the so-called "cepacia syndrome," a severe progressive respiratory failure with bacteremia that occurs in about 20% of all infected CF patients. (asm.org)
  • Since " B. cepacia " is resistant to most antimicrobial agents, effective therapies are not straightforward and management efforts are therefore aimed at prevention of infection ( 35 , 63 ). (asm.org)
  • However, the efficiency of infection control measures are determined by the accuracy with which " B. cepacia " is diagnosed, and poor laboratory proficiency in identification of this organism still prevails ( 17 , 40 , 75 ). (asm.org)
  • 2016. Burkholderia contaminans Colonization from Contaminated Liquid Docusate (Colace) in a Immunocompetent Adult with Legionnaire's Disease: Infection Control Implications and the Potential Role of Candida pellucosa. (ibcwg.org)
  • This was confirmed by several studies ( 34 , 64 , 67 , 76 , 84 , 94 ) that showed that " B. cepacia " strains can spread between CF patients via simultaneous hospital admissions or social contact outside of the hospital. (asm.org)
  • Several reports on the recovery of " B. cepacia " from CF patients appeared in the late 1970s and early 1980s ( 62 , 63 ). (asm.org)
  • As a result of these findings, new guidelines were issued to reduce the risk of " B. cepacia " acquisition. (asm.org)
  • At the beginning of this review it is essential to clarify the terminology that will be used to refer to the members of the Burkholderia cepacia complex and their relatives. (asm.org)
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