A species of gram-negative bacteria that causes disease in plants. It is found commonly in the environment and is an opportunistic pathogen in humans.
A genus of gram-negative, aerobic, rod-shaped bacteria. Organisms in this genus had originally been classified as members of the PSEUDOMONAS genus but overwhelming biochemical and chemical findings indicated the need to separate them from other Pseudomonas species, and hence, this new genus was created.
A group of phenotypically similar but genotypically distinct species (genomovars) in the genus BURKHOLDERIA. They are found in water, soil, and the rhizosphere of crop plants. They can act as opportunistic human pathogens and as plant growth promoting and biocontrol agents.
Infections with bacteria of the genus BURKHOLDERIA.
A species of BURKHOLDERIA considered to be an opportunistic human pathogen. It has been associated with various types of infections of nosocomial origin.
A species of gram-negative, aerobic bacteria that causes MELIOIDOSIS. It has been isolated from soil and water in tropical regions, particularly Southeast Asia.
A phenomenon where microorganisms communicate and coordinate their behavior by the accumulation of signaling molecules. A reaction occurs when a substance accumulates to a sufficient concentration. This is most commonly seen in bacteria.
An autosomal recessive genetic disease of the EXOCRINE GLANDS. It is caused by mutations in the gene encoding the CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR expressed in several organs including the LUNG, the PANCREAS, the BILIARY SYSTEM, and the SWEAT GLANDS. Cystic fibrosis is characterized by epithelial secretory dysfunction associated with ductal obstruction resulting in AIRWAY OBSTRUCTION; chronic RESPIRATORY INFECTIONS; PANCREATIC INSUFFICIENCY; maldigestion; salt depletion; and HEAT PROSTRATION.
Herbaceous biennial plants and their edible bulbs, belonging to the Liliaceae.
A disease of humans and animals that resembles GLANDERS. It is caused by BURKHOLDERIA PSEUDOMALLEI and may range from a dormant infection to a condition that causes multiple abscesses, pneumonia, and bacteremia.
Cyclic esters of acylated BUTYRIC ACID containing four carbons in the ring.
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
Encrustations, formed from microbes (bacteria, algae, fungi, plankton, or protozoa) embedding in extracellular polymers, that adhere to surfaces such as teeth (DENTAL DEPOSITS); PROSTHESES AND IMPLANTS; and catheters. Biofilms are prevented from forming by treating surfaces with DENTIFRICES; DISINFECTANTS; ANTI-INFECTIVE AGENTS; and antifouling agents.
A sulfanilamide that is used as an antibacterial agent.
A mitosporic Ceratobasidiaceae fungal genus that is an important plant pathogen affecting potatoes and other plants. There are numerous teleomorphs.
Proteins found in any species of bacterium.
The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.
Ability of a microbe to survive under given conditions. This can also be related to a colony's ability to replicate.
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
A species of gram-negative, aerobic bacteria that acts as both a human and plant pathogen.
Inflammation of the lung parenchyma that is associated with BRONCHITIS, usually involving lobular areas from TERMINAL BRONCHIOLES to the PULMONARY ALVEOLI. The affected areas become filled with exudate that forms consolidated patches.
The functional hereditary units of BACTERIA.
A mixture of polymyxins B1 and B2, obtained from Bacillus polymyxa strains. They are basic polypeptides of about eight amino acids and have cationic detergent action on cell membranes. Polymyxin B is used for infections with gram-negative organisms, but may be neurotoxic and nephrotoxic.
One of the FURANS with a carbonyl thereby forming a cyclic lactone. It is an endogenous compound made from gamma-aminobutyrate and is the precursor of gamma-hydroxybutyrate. It is also used as a pharmacological agent and solvent.
A contagious disease of horses that can be transmitted to humans. It is caused by BURKHOLDERIA MALLEI and characterized by ulceration of the respiratory mucosa and an eruption of nodules on the skin.
Fatty acids which are unsaturated in only one position.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
The ability of bacteria to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The genetic complement of a BACTERIA as represented in its DNA.
Those components of an organism that determine its capacity to cause disease but are not required for its viability per se. Two classes have been characterized: TOXINS, BIOLOGICAL and surface adhesion molecules that effect the ability of the microorganism to invade and colonize a host. (From Davis et al., Microbiology, 4th ed. p486)
Substances that reduce the growth or reproduction of BACTERIA.
A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.
Membrane-bound cytoplasmic vesicles formed by invagination of phagocytized material. They fuse with lysosomes to form phagolysosomes in which the hydrolytic enzymes of the lysosome digest the phagocytized material.
Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
Electron microscopy in which the ELECTRONS or their reaction products that pass down through the specimen are imaged below the plane of the specimen.
Material coughed up from the lungs and expectorated via the mouth. It contains MUCUS, cellular debris, and microorganisms. It may also contain blood or pus.
In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.
A metabolic process that converts GLUCOSE into two molecules of PYRUVIC ACID through a series of enzymatic reactions. Energy generated by this process is conserved in two molecules of ATP. Glycolysis is the universal catabolic pathway for glucose, free glucose, or glucose derived from complex CARBOHYDRATES, such as GLYCOGEN and STARCH.
Biosynthesis of GLUCOSE from nonhexose or non-carbohydrate precursors, such as LACTATE; PYRUVATE; ALANINE; and GLYCEROL.
The simplest saturated hydrocarbon. It is a colorless, flammable gas, slightly soluble in water. It is one of the chief constituents of natural gas and is formed in the decomposition of organic matter. (Grant & Hackh's Chemical Dictionary, 5th ed)
A sulfhydryl compound used to prevent urothelial toxicity by inactivating metabolites from ANTINEOPLASTIC AGENTS, such as IFOSFAMIDE or CYCLOPHOSPHAMIDE.
A genus of anaerobic, irregular spheroid-shaped METHANOSARCINALES whose organisms are nonmotile. Endospores are not formed. These archaea derive energy via formation of methane from acetate, methanol, mono-, di-, and trimethylamine, and possibly, carbon monoxide. Organisms are isolated from freshwater and marine environments.
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
An enzyme that catalyzes the conversion of urea and water to carbon dioxide and ammonia. EC 3.5.1.5.
A compound formed in the liver from ammonia produced by the deamination of amino acids. It is the principal end product of protein catabolism and constitutes about one half of the total urinary solids.
Derivatives of phenylacetic acid. Included under this heading are a variety of acid forms, salts, esters, and amides that contain the benzeneacetic acid structure. Note that this class of compounds should not be confused with derivatives of phenyl acetate, which contain the PHENOL ester of ACETIC ACID.
A mitosporic fungal species used in the production of penicillin.

BcsKC is an essential protein for the type VI secretion system activity in Burkholderia cenocepacia that forms an outer membrane complex with BcsLB. (1/74)

 (+info)

Ecological succession in long-term experimentally evolved biofilms produces synergistic communities. (2/74)

 (+info)

Inactivation of macrophage Rab7 by Burkholderia cenocepacia. (3/74)

 (+info)

The pobA gene of Burkholderia cenocepacia encodes a group I Sfp-type phosphopantetheinyltransferase required for biosynthesis of the siderophores ornibactin and pyochelin. (4/74)

 (+info)

The Burkholderia cenocepacia LysR-type transcriptional regulator ShvR influences expression of quorum-sensing, protease, type II secretion, and afc genes. (5/74)

 (+info)

Virulence of an emerging respiratory pathogen, genus Pandoraea, in vivo and its interactions with lung epithelial cells. (6/74)

 (+info)

Burkholderia cenocepacia O polysaccharide chain contributes to caspase-1-dependent IL-1beta production in macrophages. (7/74)

 (+info)

CD4+ T-cell immunity to the Burkholderia pseudomallei ABC transporter LolC in melioidosis. (8/74)

 (+info)

Burkholderia cepacia has emerged during the past 2 decades as an important opportunistic pathogen in persons with cystic fibrosis (CF) (11). Although B. cepacia was previously regarded as a single species, recent taxonomic studies have identified at least nine closely related species in what is now referred to as the B. cepacia complex (Bcc). This group includes B. cepacia, Burkholderia multivorans, Burkholderia cenocepacia, Burkholderia stabilis, Burkholderia vietnamiensis, Burkholderia dolosa, Burkholderia ambifaria, Burkholderia anthina, and Burkholderia pyrrocinia (4-6, 25, 28-32). Although each of these species has been recovered from respiratory secretions of persons with CF, B. multivorans and B. cenocepacia account for the great majority of isolates (12, 24). Mounting evidence also suggests that B. cenocepacia is somewhat more pathogenic in CF than are the remaining species (1, 8).. A feature that distinguishes Bcc from other pathogens in CF is the occurrence in a significant proportion ...
TY - JOUR. T1 - A Decade of Burkholderia cenocepacia Virulence Determinant Research. AU - Loutet, S.A.. AU - Valvano, Miguel. PY - 2010/10. Y1 - 2010/10. N2 - The Burkholderia cepacia complex (Bcc) is a group of genetically related environmental bacteria that can cause chronic opportunistic infections in patients with cystic fibrosis (CF) and other underlying diseases. These infections are difficult to treat due to the inherent resistance of the bacteria to antibiotics. Bacteria can spread between CF patients through social contact and sometimes cause cepacia syndrome, a fatal pneumonia accompanied by septicemia. Burkholderia cenocepacia has been the focus of attention because initially it was the most common Bcc species isolated from patients with CF in North America and Europe. Today, B. cenocepacia, along with Burkholderia multivorans, is the most prevalent Bcc species in patients with CF. Given the progress that has been made in our understanding of B. cenocepacia over the past decade, we ...
Mapping transcription start sites across the B. cenocepacia genome added relevant information to the J2315 annotation. Genes and novel regulatory RNAs putatively involved in B. cenocepacia biofilm formation were identified. These findings will help in understanding regulation of B. cenocepacia biofi …
The cciIR quorum-sensing system associated with epidemic strains of B. cenocepacia is involved in the regulation of known virulence factors and is a part of a regulatory network with the cepIR quorum-sensing system, since CepR is required for cciIR expression. Phylogenetic analysis demonstrated that the cepIR and cciIR systems are highly distinct. The cepIR system that is widely distributed among the Bcc was predicted to be the ancestral system, whereas the cciIR system was likely acquired by horizontal gene transfer when cci was incorporated into the B. cenocepacia genome. The presence of an ancestral and a more-recently acquired AHL-dependent quorum-sensing system has been observed in other species. The P. aeruginosa lasIR system is considered to be the ancestral system, and the rhlIR system is considered to be more recently acquired (22). The rhlIR system has been incorporated into the regulatory pathway of the lasIR system and is dependent on the lasIR system for expression (21, 34). Both of ...
Burkholderia cenocepacia ATCC ® BAA-245D-5™ Designation: Genomic DNA from Burkholderia cenocepacia strain LMG 16656 TypeStrain=False Application:
Methane is metabolized principally by methanotrophs and methanogens in the global carbon cycle. Methanotrophs consume methane as the only source of carbon, while methanogens produce methane as a metabolic byproduct. Methylotrophs, which are microorganisms that can obtain energy for growth by oxidizing one-carbon compounds, such as methanol and methane, are situated between methanotrophs and methanogens. Methanogens can obtain energy for growth by converting a limited number of substrates to methane under anaerobic conditions. Three types of methanogenic pathways are known: CO2 to methane [MD:M00567], methanol to methane [MD:M00356], and acetate to methane [MD:M00357]. Methanogens use 2-mercaptoethanesulfonate (CoM; coenzyme M) as the terminal methyl carrier in methanogenesis and have four enzymes for CoM biosynthesis [MD:M00358]. Coenzyme B-Coenzyme M heterodisulfide reductase (Hdr), requiring for the final reaction steps of methanogenic pathway, is divided into two types: cytoplasmic HdrABC in ...
Glycolysis is the process of converting glucose into pyruvate and generating small amounts of ATP (energy) and NADH (reducing power). It is a central pathway that produces important precursor metabolites: six-carbon compounds of glucose-6P and fructose-6P and three-carbon compounds of glycerone-P, glyceraldehyde-3P, glycerate-3P, phosphoenolpyruvate, and pyruvate [MD:M00001]. Acetyl-CoA, another important precursor metabolite, is produced by oxidative decarboxylation of pyruvate [MD:M00307]. When the enzyme genes of this pathway are examined in completely sequenced genomes, the reaction steps of three-carbon compounds from glycerone-P to pyruvate form a conserved core module [MD:M00002], which is found in almost all organisms and which sometimes contains operon structures in bacterial genomes. Gluconeogenesis is a synthesis pathway of glucose from noncarbohydrate precursors. It is essentially a reversal of glycolysis with minor variations of alternative paths [MD:M00003 ...
Burkholderia cenocepacia is a Gram-negative aerobic bacterium that belongs to a group of opportunistic pathogens displaying diverse environmental and pathogenic lifestyles. B. cenocepacia is known for its ability to cause lung infections in people with cystic fibrosis and it possesses a large 8 Mb multireplicon genome encoding a wide array of pathogenicity and fitness genes. Transcriptomic profiling across nine growth conditions was performed to identify the global gene expression changes made when B. cenocepacia changes niches from an environmental lifestyle to infection. In comparison to exponential growth, the results demonstrated that B. cenocepacia changes expression of over one-quarter of its genome during conditions of growth arrest, stationary phase and surprisingly, under reduced oxygen concentrations (6% instead of 20.9% normal atmospheric conditions). Multiple virulence factors are upregulated during these growth arrest conditions. A unique discovery from the comparative expression ...
Burkholderia cenocepacia is an opportunistic pathogen causing life-threatening infections in cystic fibrosis and other immunocompromised patients. The bacterium survives within macrophages by interfering with typical endocytic trafficking, resulting in delayed maturation of a B. cenocepacia-containing phagosome. We hypothesize that B. cenocepacia alters gene expression after internalization by macrophages, inducing genes involved in intracellular survival and host adaptation. Furthermore, we hypothesize that specialized bacterial secretion systems are involved in the interactions between intracellular bacteria and macrophages. In this work, we characterize later-stage infection of macrophages by B. cenocepacia, showing replication within an acidified endosomal compartment suggestive of a phagolysosome. We examine differential gene expression by intracellular B. cenocepacia using selective capture of transcribed sequences (SCOTS) with both competitive enrichment and microarray analysis. We identified 766
Since bceU is found in the genome sequences of all Bcc strains as well as in B. thailandensis, B. oklahomensis, and B. pseudomallei strains, the authors of PMID:19948863 considered it to belong as part of bce-II gene cluster ...
Purchase Recombinant Burkholderia cenocepacia ATP-dependent Clp protease adapter protein ClpS(clpS). It is produced in Yeast. High purity. Good price.
Please Note: Ortholuge cannot be used for certain RBB analyses involving closely-related strains (e.g. Burkholderia cenocepacia J2315 versus Burkholderia cenocepacia AU 1054) because the phylogenetic distance between each ingroup protein is very small in relation to the phylogenetic distance between each ingroup protein and the associated outgroup protein. In these cases, the Ortholuge Classification value will be left blank which indicates Ortholuge analysis was not peformed, however the two proteins may very well be orthologs. In other cases, only some rows will be missing Ortholuge classifications, which can occur when a suitable gene could not be found in the outgroup species ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Scientists may have found a way to kill Burkholderia cenocepacia, a multi-drug resistant plant pathogen that causes severe infections in people with cystic fibrosis.. The team of researchers has identified a weakness in the armour that protects the B. cenocepacia bacterium from the effects of antibiotics. They hypothesize that preventing the synthesis of a key sugar required for this armour, 4-amino-4-deoxy-L-arabinose (Ara4N), may lead to a susceptibility within the cell membrane to antibiotics. Researchers hope to discover novel molecules as antibiotic candidates which could disrupt the synthesis of Ara4N.. ...
Joseph D. Mougous and coworkers at the University of Washington School of Medicine and HHMI discovered a cytidine deaminase, which converts cytosine (C) to uracil (U), in the bacteria Burkholderia cenocepacia and thought the enzyme could be useful for genome editing. Mougous reached out to David R. Liu at the Broad Institute, Harvard University, and HHMI, because Lius group had previously developed base editors, which are engineered proteins that make specific single-letter changes in DNA.. ...
We report a case of a vaginal infection caused by a strain of Burkholderia cenocepacia. The strain was isolated from vaginal swab specimens from a 68-year-old woman with smoldering myeloma and chronic hepatitis C virus infection who was hospitalized ...
4B6Z: High-Resolution Structure of the M14-Type Cytosolic Carboxypeptidase from Burkholderia Cenocepacia Refined Exploiting Pdb_Redo Strategies.
Zaradi pogoste uporabe betalaktamskih zdravil so nekatere bakterije razvile odpornost proti njim. Nekatere bakterije proizvajajo prej omejeni encim betalaktamazo, ki izniči učinek betalaktamskega antibiotika, ker cepi betalaktamski obroč.. Za preprečitev tovrstne odpornosti bakterij so na trgu kombinacije betalaktamskih antibiotikov z zaviralci betalaktamaze. Takšna kombinacija je npr. amoksicilin + klavulanska kislina. Klavulanska kislina je zaviralec betalaktamaze, ker se nepovratno veže na encim in slednji se več ne more vezati na betalaktamsko učinkovino (v tem primeru amoksicilin).. ...
Download Proton 1 (Original Mix) by Tensal from the album Proton Decay. Released by Soma Records on Beatport, the worlds largest music store for DJs.
The phenylacetic acid (PA) degradative pathway is the central pathway by which complex aromatic compounds (e.g. styrene) get degraded. Upper pathways for different aromatic compounds converge at common intermediate phenylacetyl-CoA, which is then metabolized to succinyl-CoA and acetyl-CoA. We previously made a link in Burkholderia cenocepacia between phenylacetic acid (PA) degradation and virulence by showing that insertional mutagenesis of paaA and paaE results in PA-conditional growth and an attenuated killing phenotype in the Caenorhabditis elegans model of infection. Insertional mutagenesis of paaK1, which encodes a phenylacetyl-CoA ligase, did not result in a PA-conditional growth probably due to the presence of a second similar gene (paaK2) encoding another phenylacetyl-CoA ligase (PaaK2). Recently published crystallographic and enzyme kinetics studies comparing the two ligases show that PaaK1 is better able to bind hydroxylated PA derived molecules such as 3-hydroxyphenylacetic (3-OHPA) acid and
Essential gene studies often reveal novel essential functions for genes with dispensable homologues in other species. This is the case with the widespread family of electron transfer flavoproteins (ETFs), which are required for the metabolism of specific substrates or for symbiotic nitrogen fixation in some bacteria. Despite these non-essential functions high-throughput screens have identified ETFs as putatively essential in several species. In this study, we constructed a conditional expression mutant of one of the ETFs in Burkholderia cenocepacia, and demonstrated that its expression is essential for growth on both complex media and a variety of single-carbon sources. We further demonstrated that the two subunits EtfA and EtfB interact with each other, and that cells depleted of ETF are non-viable and lack redox potential. These cells also transition from the short rods characteristic of Burkholderia cenocepacia to small spheres independently of MreB. The putative membrane partner ETF dehydrogenase
SWISS-MODEL Repository entry for A0K321 (GCSP_BURCH), Glycine dehydrogenase (decarboxylating). Burkholderia cenocepacia (strain HI2424)
Acylhomoserine lactone (AHL) analogues in which the amide function is replaced by a triazole group were synthesized and evaluated for their effect on quorum sensing (QS) and biofilm formation in Burkholderia cenocepacia and Pseudomonas aeruginosa. In addition, the influence of the length of the acyl-mimicking chain was investigated. The compounds showed selectivity between two different AHL QS systems. 3-(1H-1,2,3-Triazol-1-yl)dihydrofuran-2(3H)-ones, in which the 4-substituent best resembled the acyl chain of the native AHL molecule exhibited significant QS agonistic and antagonistic activities. Replacing this aliphatic substituent by a phenyl-containing moiety resulted in active inhibitors of QS. The most active compounds showed biofilm inhibitory as well as biofilm eradicating activities in both test organisms. ...
DNA microarrays have proven powerful for functional genomics studies. Several technologies exist for the generation of whole-genome arrays. It is well documented that 25mer probes directed against different regions of the same gene produce variable signal intensity values. However, the extent to which this is true for probes of greater length (60mers) is not well characterized. Moreover, this information has not previously been reported for whole-genome arrays designed against bacteria, whose genomes may differ substantially in characteristics directly affecting microarray performance. We report here an analysis of alternative 60mer probe designs for an in-situ synthesized oligonucleotide array for the GC rich, β-proteobacterium Burkholderia cenocepacia. Probes were designed using the ArrayOligoSel3.5 software package and whole-genome microarrays synthesized by Agilent, Inc. using their in-situ, ink-jet technology platform. We first validated the quality of the microarrays as demonstrated by an average
Survival curves for D. melanogaster infected with B. cenocepacia strains.Pricking assays were performed with a minimum of 30 flies for each strain. Statistical
The Össur Academy team has a strong professional commitment to render the best possible services to our customers. Comprised of well educated professionals, all team members are dedicated to the goals of the Össur Academy: To further the level of education and quality of prosthetic and orthopedic knowledge among professionals, patients and their families.
CEPI is an alliance to finance and coordinate the development of new vaccines to prevent and contain infectious disease epidemics.
Gentaur molecular products has all kinds of products like :search , ATGen \ BCAS2, 1_225aa, Human, His tag, E.coli \ ATGP0914 for more molecular products just contact us
Copyright The Student Room 2017 all rights reserved The Student Room, Get Revising and Marked by Teachers are trading names of The Student Room Group Ltd.. Register Number: 04666380 (England and Wales), VAT No. 806 8067 22 Registered Office: International House, Queens Road, Brighton, BN1 3XE ...
TY - CONF. T1 - Comparison of biofilm formation between Burkholderia multivorans Burkholderia cenocepacia Pseudomonas aeruginosa in the presence or absence of mucin. AU - Einarsson, Gisli. AU - McDowell, Andrew. AU - Elborn, Joseph. AU - Martin, Lorraine. PY - 2009/6. Y1 - 2009/6. M3 - Paper. SP - S34-S34. T2 - European Cystic Fibrosis Conference. Y2 - 1 June 2009 through 1 June 2009. ER - ...
Aabed, Tariq Ahmad (2018) Radiation Effects on Mesenchymal Stem Cells in a Model of Fibrosarcoma. PhD thesis, University of Sheffield. Albaz, Alrayan (2018) Investigation into Cytosine Epigenetic State in Bladder Cancer. PhD thesis, University of Sheffield. Aljadani, Saeedah (2018) Regulation of iron acquisition by an iron starvation sigma factor system in Burkholderia cenocepacia. PhD thesis, University of Sheffield. Alkathiri, Afnan (2018) Investigation the Role of ATM in Sarcomas. PhD thesis, University of Sheffield. Allocca, Gloria (2018) Defining the perivascular niche in the early stages of breast cancer bone metastasis. PhD thesis, University of Sheffield. Almaghrabi, Sarah (2018) Gene Therapy for an Experimental Autoimmune Polyglandular Syndrome 1 Model Caused by Mutations in Aire. PhD thesis, University of Sheffield. Alrahimi, Jehan (2018) The Role of Tetraspanins in Pseudomonas aeruginosa Adherence to Human Cells. PhD thesis, University of Sheffield. Alshamrani, Khalaf (2018) ...
This pipeline reflects, to the best of our knowledge, publicly available information on the status of vaccine candidates with a direction towards human use for Lassa and Nipah (as at August 2017) and for MERS-CoV (as at July 2017). Please contact [email protected] if you have evidence to report on updates to this.. ...
Össur is a trusted supplier of the NHS, having supplied supports and braces for medical and injury supports for over 10 years. Free standard delivery on all orders.
Zaviralci proteaz ali proteazni zaviralci (oznaka ATC: J05AE) so skupina učinkovin za zdravljenje ali preprečevanje virusnih okužb, vključno z okužbami z virusom HIV in virusom hepatitisa C. Preprečujejo virusno podvojevanje, tako da zavrejo delovanje encima proteaze (npr. proteaze HIV-1, ki cepi nastajajoče beljakovine, ki se nato združujejo in tvorijo virione). Doslej so razvili oziroma so v fazi razvoja proteazni zaviralci za naslednje virusne okužbe:: ...
General Information: Burkholderia cenocepacia MC0-3 was isolated from soil associated with maize roots. This species (genomovar III) is part of the Burkholderia cepacia complex, which consists of 9 closely related species, also known as genomovars. These organisms are commonly found in soil and all are opportunistic pathogens espcially in cystic fibrosis patients. ...
Chronic pulmonary infections are the principal cause of morbidity and mortality in individuals with cystic fibrosis (CF). Due to the polymicrobial nature of these infections, the identification of the particular bacterial species responsible is an essential step in diagnosis and treatment. Current diagnostic procedures are time-consuming, and can also be expensive, invasive and unpleasant in the absence of spontaneously expectorated sputum. The development of a rapid, non-invasive methodology capable of diagnosing and monitoring early bacterial infection is desired. Future visions of real-time, in situ diagnosis via exhaled breath testing rely on the differentiation of bacteria based on their volatile metabolites. The objective of this proof-of-concept study was to investigate whether a range of CF-associated bacterial species (i.e. Pseudomonas aeruginosa, Burkholderia cenocepacia, Haemophilus influenzae, Stenotrophomonas maltophilia, Streptococcus pneumoniae and Streptococcus milleri) could be ...
The RNA Printer™ is capable of producing several grams of LNP-formulated mRNA (enough to produce more than a hundred thousand doses), within just a few weeks. This platform can also produce mRNA vaccine candidates against multiple pathogens using the same technology, saving time and reducing costs compared with other vaccine platforms.. CureVacs vaccine platform could be a game-changer, radically improving our ability to respond to the emergence of Disease X, said Richard Hatchett, CEO of CEPI. Disease X could emerge suddenly and have deadly consquences-weve seen this happen with Ebola, MERS coronavirus, Zika, and countless other diseases. Thats why were striving to develop rapid-response vaccine platforms-like CureVacs mRNA technology-to defend against these unknown pathogens. CEPI has now established partnership agreements totaling more than $50 million in three such platforms.. CureVacs mRNA technology can be designed to encode for many proteins or antigens, offering rich ...
Ah, yes! Im dealing with Burkholderia. 16s rDNA analyses showed similarity to B. cepacia and B. cenocepacia. Perhaps the fact that B. cepacia exists as a complex of many strains (genomovars) that makes biochemical identification inaccurate; what more when I did it the conventional way. Hoping this time that the commercial kit like API could back the 16s analyses up.. ...
Blog on BCAS2 recombinant protein product: The BCAS2 bcas2 (Catalog #MBS203900) is a Recombinant Protein and is intended for research purposes only....
A Global leader in orthopaedics, Össur employs the smartest minds and the most advanced technologies to help keep people mobile.
Co-led by Gavi, the Coalition for Epidemic Preparedness Innovations (CEPI) and WHO, COVAX aims to accelerate the development and manufacture of COVID-19 vaccines while guaranteeing fair and equitable access for every country.
The Burkholderia cepacia complex consists of nine closely related species: Burkholderia cepacia genomovars I and VI, Burkholderia multivorans (genomovar II), Burkholderia cenocepacia (genomovar III), Burkholderia stabilis (genomovar IV), Burkholderia vietnamiensis (genomovar V), Burkholderia ambifaria (genomovar VII), Burkholderia anthina (genomovar VIII), and Burkholderia pyrrocinia (genomovar IX) (2, 9, 10). Despite the advances that have been made in the understanding of the taxonomy and epidemiology of this group, infections of the respiratory tract with B. cepacia complex organisms still have a considerable impact on morbidity and mortality in cystic fibrosis (CF) patients (4, 5, 6). B. cepacia complex strains have also attracted attention as possible biocontrol and bioremediation agents that could be used as antagonists of soil-borne plant pathogens, as plant growth-promoting agents, and for the bioremediation of various recalcitrant xenobiotics (3, 8). To aid in identification, ...
Bacteria of the Burkholderia cepacia complex (Bcc) are pathogens of humans, plants, and animals. Burkholderia cenocepacia is one of the most common Bcc species infecting cystic fibrosis (CF) patients and its carriage is associated with poor prognosis. In this study, we characterized a general O-link …
A plate assay to detect bacterial lipase (EC 3.1.1.3) in a medium containing trioleoylglycerol and the fluorescent dye rhodamine B is presented. Substrate hydrolysis causes the formation of orange fluorescent halos around bacterial colonies visible upon UV irradiation. The logarithm of lipase activity from cell-free culture supernatants is linearly correlated with the diameter of halos, thereby allowing quantitation of lipase activities ranging from 1 to 30 nkat. ...
Looking for online definition of BCAM or what BCAM stands for? BCAM is listed in the Worlds largest and most authoritative dictionary database of abbreviations and acronyms
Reagents, Tools and Custom Services for molecular biology, specializing in the fields of Nano-Antibody development (nAb), Cellular Reprogramming (iPSC), Genome Editing, Fluorescent Proteins, RNAi, Viral Packaging and Protein expression.
CEPI: Part of the Problem or the Solution?. Certainly, dont tell a New World Order organization called the Coalition for Epidemic Preparedness Innovations (CEPI) about this coronavirus cure. CEPI is handing out research grants to develop a vaccine. This is free money to pharmaceutical and biotech companies and actually the whole reason for the conjured-up epidemic, to develop a vaccine with public money.. CEPI is a private/public coalition headquartered in Norway with $760-billion of funding by various countries, including the Wellcome Trust and the Bill & Melinda Gates Foundation.. Note: Burroughs Wellcome & Co. (gift to 650 newspapermen at the First Imperial Press Conference, 1909, bankrolled by Harry Wellcome, whose Empire Press Union, 1909 newspapermen created British MI6, MI5 and CG&CS renamed GCHQ in 1946 with the founding of Five Eyes (UK, US, CA, NZ, and AU Commonwealth secret intelligence sharing)-wait, the U.S. is sovereign, right? Wrong. More at ...
Regan KH, Bhatt J. Eradication therapy for Burkholderia cepacia complex in people with cystic fibrosis. Cochrane Database of Systematic Reviews 2016, Issue 11. Art. No.: CD009876. DOI: 10.1002/14651858.CD009876.pub3 ...
Pulmonary infection with Burkholderia cepacia complex (BCC) in patients with CF is often associated with a more rapid decline in lung function. Because of the resistance of BCC to many antibiotics, treatment options are often limited. New therapies to improve outcomes for patients infected with BCC are needed.. However, because of the unpredictable nature of this pulmonary infection in CF, patients with BCC infection have been excluded from many CF therapeutic trials.. Recent published trials in the United States, Australia, and the United Kingdom have all demonstrated clinical benefits from prolonged administration of azithromycin in CF. In these trials, the vast majority of patients were chronically infected with Pseudomonas aeruginosa.. Patients with BCC were excluded from the US and UK trials, and only four patients with BCC infection were enrolled in the Australian trial. Thus, the effectiveness of azithromycin in CF patients infected with BCC is largely unknown and deserves further ...
Burkholderia cepacia complex answers are found in the Johns Hopkins ABX Guide powered by Unbound Medicine. Available for iPhone, iPad, Android, and Web.
The fact that the 2030 Energy and Climate package includes an analysis of energy costs and was published together with the European Industrial Renaissance Communication is a landmark shift in EU policy. The Confederation of European Paper Industries (CEPI) interprets this as a signal showing the European Commission is starting to take industrial competitiveness seriously. However, there are still important steps to be taken.. In practice, this new package will not change the competitiveness of industry in the short run. The high pile of documents released by the Commission analyses and promises, but at the end it only proposes one legal act - the change of the EU ETS. In this regard, CEPI welcomes the Commission proposal to keep EU ETS unchanged up to 2020 to give the necessary stability for investments in EU industry. But is this enough?. The Commission finally acknowledged energy prices in Europe are uncompetitive, as electricity costs for industry are twice that of US competitors. It also ...
General Information: This organism was originally isolated from a rice field sample in Thailand. Burkholderia thailandensis is a common soil saprophyte (lives on decaying organic matter in the soil). This bacterium is very similar to the human and animal pathogen Burkholderia pseudomallei but appears to be avirulent. Distinguishing the two organisms is very difficult and may depend on using monoclonal antibodies to detect differences in exopolysaccharide production. ...
A World Economic Forum publication in collaboration with industry The European paper industry was invited to collaborate with the World Economic Forum (the Forum), the Ellen MacArthur Foundation and the McKinsey Center for Business and Environment on Circular Economy to produce a white paper with guidelines on design and management for circularity. The new publication provides essential guidance to all actors in the supply chain through simple ecodesign rules for paper products, without limiting innovation and the introduction of new techniques. This is a product of the three pilots under Project MainStream, launched during the 2014 summit in Davos.. We trust helping circular thinking in all steps of the complex value chain will help reach higher in what is already a high recycling performance, said CEPI Sustainability Director Jori Ringman, one of the draftsmen of the guidance, in a panel discussion on the feasibility of higher recycling rates at the Packaging and Sustainability event in ...
Expression of BCAS1 (AIBC1, NABC1) in cancer tissue. The cancer tissue page shows antibody staining of the protein in 20 different cancers.
... biofilms are present in the chronic Pseudomonas aeruginosa and Burkholderia cenocepacia infections characteristic of cystic ...
Burkholderia cenocepacia. *Burkholderia gladioli. C. *Campylobacter. *Chlamydia trachomatis. *Chlamydiae. *Chlorobi. * ...
Biofilm dapat ditemui pada banyak infeksi, seperti infeksi Pseudomonas aeruginosa kronis dan Burkholderia cenocepacia, yang ...
Burkholderia cenocepacia, na Mycobacterium avium ni ni pathojeni ambazo hutaka kutumia hali fulani ya binadamu walio na uhaba ...
Burkholderia cenocepacia, and Mycobacterium avium, are opportunistic pathogens and cause disease mainly in people suffering ...
Genomic DNA from Burkholderia cenocepacia strain LMG 16656 TypeStrain=False Application: ... Burkholderia cenocepacia (ATCC® BAA-245D-5™) Strain Designations: Genomic DNA from Burkholderia cenocepacia strain LMG 16656 [ ... Burkholderia cepacia (Palleroni and Holmes) Yabuuchi et al. Strain Designations Genomic DNA from Burkholderia cenocepacia ... Burkholderia cenocepacia ATCC® BAA-245D-5™ dried Total DNA: At least 5 µg in 1X TE buffer ...
Glycolysis / Gluconeogenesis - Burkholderia cenocepacia J2315 [ Pathway menu , Organism menu , Pathway entry , Download KGML , ...
Methane metabolism - Burkholderia cenocepacia J2315 [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show ...
Genes and novel regulatory RNAs putatively involved in B. cenocepacia biofilm formation were identified. These findings will ... help in understanding regulation of B. cenocepacia biofi … ... cenocepacia genome added relevant information to the J2315 ... Genome-wide transcription start site profiling in biofilm-grown Burkholderia cenocepacia J2315 BMC Genomics. 2015 Oct 13;16:775 ... Background: Burkholderia cenocepacia is a soil-dwelling Gram-negative Betaproteobacterium with an important role as ...
sp,A0K578,URE3_BURCH Urease subunit gamma OS=Burkholderia cenocepacia (strain HI2424) OX=331272 GN=ureA PE=3 SV=1 ... Burkholderia cenocepacia (strain HI2424). ,p>This subsection of the ,a href="http://www.uniprot.org/help/names_and_taxonomy_ ... cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › ...
Characterization of the cciIR Quorum-Sensing System in Burkholderia cenocepacia. Rebecca J. Malott, Adam Baldwin, Eshwar ... Characterization of the cciIR Quorum-Sensing System in Burkholderia cenocepacia. Rebecca J. Malott, Adam Baldwin, Eshwar ... Characterization of the cciIR Quorum-Sensing System in Burkholderia cenocepacia. Rebecca J. Malott, Adam Baldwin, Eshwar ... Several transmissible Burkholderia cenocepacia strains that infect multiple cystic fibrosis patients contain a genomic island ...
... cenocepacia-containing phagosome. We hypothesize that B. cenocepacia alters gene expression after internalization by ... In this work, we characterize later-stage infection of macrophages by B. cenocepacia, showing replication within an acidified ... As a whole, secretion systems appear to increase pathogenicity in macrophages while limiting the spread of B. cenocepacia ... Expression of secretion system genes is differentially-regulated by intracellular B. cenocepacia. Although none of the five ...
title = "A Decade of Burkholderia cenocepacia Virulence Determinant Research",. abstract = "The Burkholderia cepacia complex ( ... A Decade of Burkholderia cenocepacia Virulence Determinant Research. Together they form a unique fingerprint. * Burkholderia ... A Decade of Burkholderia cenocepacia Virulence Determinant Research. In: Infection and Immunity. 2010 ; Vol. 78, No. 10. pp. ... A Decade of Burkholderia cenocepacia Virulence Determinant Research. Infection and Immunity. 2010 Oct;78(10):4088-4100. https ...
The Burkholderia Genome Database: facilitating flexible queries and comparative analyses. Bioinformatics 2008 Dec 1;24(23):2803 ... If you have used this database, please ensure that you acknowledge the Burkholderia Genome Database publication rather than ... Distribution of cepacian biosynthesis genes among environmental and clinical Burkholderia strains and role of cepacian ... Burkholderia cenocepacia H111, I35_RS21130 (bceU) .list-group { margin-bottom: 5px; width: 150px; text-align: left; } ...
Burkholderia cenocepacia is a Gram-negative aerobic bacterium that belongs to a group of opportunistic pathogens displaying ... The unexpected discovery of a novel low-oxygen-activated locus for the anoxic persistence of Burkholderia cenocepacia. ISME ... The unexpected discovery of a novel low-oxygen-activated locus for the anoxic persistence of Burkholderia cenocepacia ... In summary, transcriptomic profiling of B. cenocepacia revealed an unexpected ability of aerobic Burkholderia to persist in the ...
Burkholderia cenocepacia Vaginal Infection in Patient with Smoldering Myeloma and Chronic Hepatitis C ... We report a case of a vaginal infection caused by a strain of Burkholderia cenocepacia. The strain was isolated from vaginal ... Members of the Burkholderia cepacia complex (Bcc), found in many environments, are associated with clinical infections. ... We report the first case of community-acquired bacteremia with Burkholderia fungorum, a newly described member of the ...
Scientists may have found a way to kill Burkholderia cenocepacia, a multi-drug resistant plant pathogen that causes severe ... The team of researchers has identified a weakness in the armour that protects the B. cenocepacia bacterium from the effects of ...
... in the bacteria Burkholderia cenocepacia and thought the enzyme could be useful for genome editing. Mougous reached out to ... B. cenocepacia make the protein as a weapon against other bacteria, so the protein on its own is toxic to cells. "We quickly ...
Burkholderia cenocepacia sp. nov.--a new twist to an old story.. Vandamme P1, Holmes B, Coenye T, Goris J, Mahenthiralingam E, ... DNA-DNA hybridisation experiments between isolates representing Burkholderia cepacia genomovar III recA lineages IIIA and IIIB ... cenocepacia sp. nov., with LMG 16656 as the type strain, is proposed. ...
sp,A0AYH4,ASSY_BURCH Argininosuccinate synthase OS=Burkholderia cenocepacia (strain HI2424) OX=331272 GN=argG PE=3 SV=1 ... Burkholderia cenocepacia (strain HI2424). ,p>This subsection of the ,a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy ... cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › ...
Burkholderia cenocepacia J2315. Mutation(s): 0 Gene Names: BCAL0763. Find proteins for B4EA88 (Burkholderia cenocepacia (strain ... The structure of a nutrient binding protein from Burkholderia cenocepacia bound to methionine. Clifton, M.C., Arakaki, T.. To ... The structure of a nutrient binding protein from Burkholderia cenocepacia bound to methionine. *DOI: 10.2210/pdb4QHQ/pdb ...
Burkholderia cenocepacia is a member of the B. cepacia complex, a diverse group of bacteria with important clinical ... J. J. LiPuma, and P. Vandamme, 2004 Recovery of Burkholderia cenocepacia strain PHDC from cystic fibrosis patients in Europe. ... The Rate and Molecular Spectrum of Spontaneous Mutations in the GC-Rich Multichromosome Genome of Burkholderia cenocepacia. ... The Rate and Molecular Spectrum of Spontaneous Mutations in the GC-Rich Multichromosome Genome of Burkholderia cenocepacia. ...
Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov., ... Burkholderia cenocepacia and Burkholderia multivorans: influence on survival in cystic fibrosis. Thorax 59:948-951. ... CDSs with matches identified by reciprocal FASTA with other Burkholderia species-B. cenocepacia HI2424, B. cenocepacia AU1054, ... cenocepacia island (CCI), are more frequently associated with B. cenocepacia IIIA strains than other B. cenocepacia recA ...
Find proteins for B4EA43 (Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610)) ... Burkholderia cenocepacia. Mutation(s): 0 Gene Names: nagZ, A3203_21235, WL84_04775. EC: 3.2.1.52. ... Crystal structure of Burkholderia cenocepacia family 3 glycoside hydrolase (NagZ) bound to (3S,4R,5R,6S)-3-butyryl-4,5,6- ...
Treatment with piperacillin/tazobactam eliminated B. cenocepacia infection and vaginal symptoms ... We report a case of a vaginal infection caused by a strain of Burkholderia cenocepacia. The strain was isolated from vaginal ... Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, Burkholderia cepacia genomovars I and III. J Clin ... Burkholderia anthina sp. nov. and Burkholderia pyrrocinia, two additional Burkholderia cepacia complex bacteria, may confound ...
Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Burkholderia; Burkholderia cepacia complex. ... Whole-Genome Assemblies of 56 Burkholderia Species.. Journal. Genome Announc 2:e01106-14 (2014). DOI: 10.1128/genomeA.01106-14 ...
Free resource for searching and exporting immune epitopes. Includes more than 95% of all published infectious disease, allergy, autoimmune, and transplant epitope data.
Burkholderia cenocepacia sp. nov.-a new twist to an old story. Res. Microbiol. 154 : 91-96. ... Adherence and autoaggregation phenotypes of a Burkholderia cenocepacia cable pilus mutant. FEMS Microbiol. Lett. 228 : 287-297. ... Transcriptional and posttranscriptional control of cable pilus gene expression in Burkholderia cenocepacia. J. Bacteriol. 186 : ... B. cenocepacia (formerly B. cepacia complex genomovar III) strain BC7 is a cable-piliated CF clinical isolate of B. cenocepacia ...
In this work, we investigated the lipopolysaccharide (LPS) of clinical variants of B. cenocepacia that were collected from a ... In this work, we investigated the lipopolysaccharide (LPS) of clinical variants of B. cenocepacia that were collected from a ... cenocepacia. The IST439 OAg biosynthetic gene cluster contains 7 of 23 genes that are closely homologous to genes found in B. ... cenocepacia. The IST439 OAg biosynthetic gene cluster contains 7 of 23 genes that are closely homologous to genes found in B. ...
We previously made a link in Burkholderia cenocepacia between phenylacetic acid (PA) degradation and virulence by showing that ... induces the phenylacetic acid degradative pathway in Burkholderia cenocepacia K56-2. Using reporter activity assays, we show ... induces the phenylacetic acid degradative pathway in Burkholderia cenocepacia K56-2. Using reporter activity assays, we show ... We previously made a link in Burkholderia cenocepacia between phenylacetic acid (PA) degradation and virulence by showing that ...
Identification of Essential Operons with a Rhamnose-Inducible Promoter in Burkholderia cenocepacia. Silvia T. Cardona, Carmen L ... Identification of essential genes and essential operons in B. cenocepacia K56-2.Based on the previous results, B. cenocepacia ... Identification of Essential Operons with a Rhamnose-Inducible Promoter in Burkholderia cenocepacia ... Identification of Essential Operons with a Rhamnose-Inducible Promoter in Burkholderia cenocepacia ...
Burkholderia cenocepacia) (OPCA05248) , Alias: UDP-3-O-[R-3-hydroxymyristoyl]-N-acetylglucosamine deacetylase , MW: 51.00630092 ... Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia ( ... The genome of Burkholderia cenocepacia J2315, an epidemic pathogen of cystic fibrosis patients.. Holden M.T., Seth-Smith H.M., ...
σ54-Dependent Response to Nitrogen Limitation and Virulence in Burkholderia cenocepacia Strain H111. Martina Lardi, Claudio ... S1); consensus sequence logo for the B. cenocepacia H111 σ54-binding site (Fig. S2); growth delay of the B. cenocepacia H111 σ ... σ54-Dependent Response to Nitrogen Limitation and Virulence in Burkholderia cenocepacia Strain H111 ... σ54-Dependent Response to Nitrogen Limitation and Virulence in Burkholderia cenocepacia Strain H111 ...
A Burkholderia cenocepacia orphan LuxR homolog is involved in quorum-sensing regulation. J. Bacteriol. 191:2447-2460. ... Identification of Burkholderia cenocepacia Strain H111 Virulence Factors Using Nonmammalian Infection Hosts. Stephan Schwager, ... Burkholderia cenocepacia ZmpB is a broad-specificity zinc metalloprotease involved in virulence. Infect. Immun. 74:4083-4093. ... Burkholderia cenocepacia H111, a strain isolated from a cystic fibrosis patient, has been shown to effectively kill the ...
Burkholderia cenocepacia, an opportunistic pathogen, produces a mixture of tetra- and penta-acylated lipid A. Late ... The LpxL Acyltransferase Is Required for Normal Growth and Penta-Acylation of Lipid A in Burkholderia Cenocepacia Yasmine Fathy ... The LpxL Acyltransferase Is Required for Normal Growth and Penta-Acylation of Lipid A in Burkholderia Cenocepacia Yasmine Fathy ... Burkholderia cenocepacia, an opportunistic pathogen, produces a mixture of tetra- and penta-acylated lipid A. "Late" ...
Quorum-sensing mutations affect attachment and stability of Burkholderia cenocepacia biofilms. Applied and Environmental ...
Time-kill analysis of a stationary phase culture of Burkholderia cenocepacia strain LMG 16656 when exposed to geraniol (0.25%, ... Time-kill analysis of a stationary phase culture of Burkholderia cenocepacia strain LMG 16656 when exposed to geraniol (0.25%, ... Burkholderia cepacia gas chromatography analysis MBC GC ...
Efflux pump genes of the resistance-nodulation-division family in Burkholderia cenocepacia genome. BMC Microbiol. 6:66. doi: ... Deciphering the role of RND efflux transporters in Burkholderia cenocepacia. PLoS One 6:e18902. doi:10.1371/journal.pone. ... Involvement of toxin-antitoxin modules in Burkholderia cenocepacia biofilm persistence. Pathog. Dis. 71:326-335. doi:10.1111/ ... Evaluation of the efficacy of disinfection procedures against Burkholderia cenocepacia biofilms. J. Hosp. Infect. 70:361-368. ...
Identification of a novel virulence factor in Burkholderia cenocepacia H111 required for efficient slow killing of ... "Identification of a Novel Virulence Factor in Burkholderia Cenocepacia H111 Required for Efficient Slow Killing of ... Identification of a novel virulence factor in Burkholderia cenocepacia H111 required for efficient slow killing of ... "Identification of a Novel Virulence Factor in Burkholderia Cenocepacia H111 Required for Efficient Slow Killing of ...
B. cenocepacia utilizes a mechanism of cell-cell communication called quorum sensing to control gene expression including genes ... The B. cenocepacia quorum sensing network includes the CepIR and CciIR regulatory systems. Global gene expression profiles ... For the first time, the influence of CciIR on global gene regulation in B. cenocepacia has been elucidated. Novel genes under ... cenocepacia. A comparison of CepIR-regulated genes identified in previous studies and in the current study showed a substantial ...
  • Over the past 20 years a group of closely related Burkholderia species termed the B. cepacia complex (Bcc) have emerged as opportunistic pathogens of particular importance in people with cystic fibrosis (CF) and chronic granulomatous disease ( 31 , 33 ). (asm.org)
  • The Burkholderia cepacia complex (Bcc) is a group of genetically related environmental bacteria that can cause chronic opportunistic infections in patients with cystic fibrosis (CF) and other underlying diseases. (qub.ac.uk)
  • B. cenocepacia harbours a large, multi-replicon genome with a high GC-content, the reference genome of strain J2315 includes 7374 annotated genes. (nih.gov)
  • RNA extracted from B. cenocepacia J2315 biofilms was analysed by differential RNA-sequencing and the resulting dataset compared to data derived from conventional, global RNA-sequencing. (nih.gov)
  • Mapping transcription start sites across the B. cenocepacia genome added relevant information to the J2315 annotation. (nih.gov)
  • AY278465 Burkholderia cenocepacia strain LMG 16656 outer membrane lipoprotein precursor (opcL) gene, partial cds. (atcc.org)
  • Genes and novel regulatory RNAs putatively involved in B. cenocepacia biofilm formation were identified. (nih.gov)
  • These findings will help in understanding regulation of B. cenocepacia biofilm formation. (nih.gov)
  • In this work, we characterize later-stage infection of macrophages by B. cenocepacia , showing replication within an acidified endosomal compartment suggestive of a phagolysosome. (uwo.ca)
  • As a whole, secretion systems appear to increase pathogenicity in macrophages while limiting the spread of B. cenocepacia infection. (uwo.ca)
  • Loutet, SA & Valvano, M 2010, ' A Decade of Burkholderia cenocepacia Virulence Determinant Research ', Infection and Immunity , vol. 78, no. 10, pp. 4088-4100. (qub.ac.uk)
  • Expression of secretion system genes is differentially-regulated by intracellular B. cenocepacia . (uwo.ca)
  • Several transmissible Burkholderia cenocepacia strains that infect multiple cystic fibrosis patients contain a genomic island designated as the c eno c epacia i sland (cci). (asm.org)
  • Burkholderia cenocepacia is an opportunistic pathogen causing life-threatening infections in cystic fibrosis and other immunocompromised patients. (uwo.ca)
  • The bacterium survives within macrophages by interfering with typical endocytic trafficking, resulting in delayed maturation of a B. cenocepacia -containing phagosome. (uwo.ca)
  • We hypothesize that B. cenocepacia alters gene expression after internalization by macrophages, inducing genes involved in intracellular survival and host adaptation. (uwo.ca)
  • Deletion of various SCOTS-identified genes affects B. cenocepacia entry into macrophages and intracellular replication, as well as host-directed cytotoxicity and spread to neighbouring cells. (uwo.ca)
  • Together, these studies advance our understanding of the intracellular behaviour of B. cenocepacia in macrophages. (uwo.ca)
  • We examine differential gene expression by intracellular B. cenocepacia using selective capture of transcribed sequences (SCOTS) with both competitive enrichment and microarray analysis. (uwo.ca)
  • Further investigation into the remaining SCOTS-identified genes, as well as putative secreted effectors, will provide a better understanding of the adaptive responses of intracellular B. cenocepacia , leading to life in a phagosomal niche and host cell cytotoxicity. (uwo.ca)
  • Four thousand ten transcription start sites were mapped over the whole B. cenocepacia genome and the primary transcription start site of 2089 genes expressed in B. cenocepacia biofilms were defined. (nih.gov)
  • Distribution of cepacian biosynthesis genes among environmental and clinical Burkholderia strains and role of cepacian exopolysaccharide in resistance to stress conditions. (burkholderia.com)
  • Infections with B. cenocepacia are very difficult to treat due to their high intrinsic resistance to most antibiotics. (nih.gov)
  • A common theme emerging from these studies is that B. cenocepacia establishes chronic infections in immuno-compromised patients, which depend more on determinants mediating host niche adaptation than those involved directly in host cells and tissue damage. (qub.ac.uk)
  • B. cenocepacia strains also have a cepIR quorum-sensing system. (asm.org)
  • The majority of transmissible or epidemic Bcc strains are B. cenocepacia ( 26 , 31 , 48 , 59 ), and epidemiological studies have shown this species is highly virulent, causing significant mortality among CF patients ( 28 , 30 ). (asm.org)
  • B. cenocepacia is the most commonly reported Bcc species isolated from patients with CF ( 26 , 48 ). (asm.org)
  • Burkholderia cenocepacia has been the focus of attention because initially it was the most common Bcc species isolated from patients with CF in North America and Europe. (qub.ac.uk)
  • Burkholderia cenocepacia is a soil-dwelling Gram-negative Betaproteobacterium with an important role as opportunistic pathogen in humans. (nih.gov)
  • The distribution of internal transcription start sites can be used to identify genomic islands in B. cenocepacia. (nih.gov)
  • 5: 83 Abstract: BACKGROUND: Burkholderia cenocepacia is a threatening nosocomial epidemic pathogen in patients with cystic fibrosis (CF) or a compromised immune system. (omicsdi.org)
  • CC Burkholderia cenocepacia HI2424 plasmid 1, complete sequence. (univ-lyon1.fr)
  • Burkholderia cenocepacia is a soil-dwelling Gram-negative Betaproteobacterium with an important role as opportunistic pathogen in humans. (nih.gov)
  • Here, we present a genome-wide view of the molecular mutation spectrum in Burkholderia cenocepacia , a clinically relevant pathogen with high %GC content and multiple chromosomes. (genetics.org)
  • Burkholderia cenocepacia is an opportunistic pathogen associated with chronic lung infections and increased risk of death in patients with cystic fibrosis (CF). In this work, we investigated the lipopolysaccharide (LPS) of clinical variants of B. cenocepacia that were collected from a CF patient over a period of 3.5 years, from the onset of infection until death by necrotizing pneumonia (cepacia syndrome). (frontiersin.org)
  • Burkholderia cenocepacia is an opportunistic pathogen causing life-threatening infections in cystic fibrosis and other immunocompromised patients. (uwo.ca)
  • Burkholderia cenocepacia, an opportunistic pathogen, produces a mixture of tetra- and penta-acylated lipid A. "Late" acyltransferases add secondary acyl chains to lipid A after the incorporation of four primary acyl chains to the diglucosamine backbone. (nih.gov)
  • Burkholderia cenocepacia is recognized as opportunistic pathogen that can cause lung infections in cystic fibrosis patients. (biomedcentral.com)
  • Here, we used RNA-seq to comprehensively and quantitatively assess the transcriptional response of the bacterial opportunistic cystic fibrosis (CF) pathogen and endemic soil dweller, Burkholderia cenocepacia, in conditions mimicking these 2 environments. (omicsdi.org)
  • B. cenocepacia is an opportunistic human pathogen that is particularly problematic for patients suffering from cystic fibrosis. (omicsdi.org)
  • Further analyses showed that Candida albicans germ tube formation was strongly inhibited by either coculture with B. cenocepacia or by exogenous addition of physiological relevant levels of BDSF, whereas deletion of Bcam0581 abrogated the inhibitory ability of the bacterial pathogen. (pubfacts.com)
  • In summary, we have demonstrated for the first time that IS movement can be mediated by oxidative stress and can lead to genomic rearrangements in the CF pathogen B. cenocepacia. (plymouth.ac.uk)
  • Burkholderia cenocepacia is an opportunistic pathogen prevalent in cystic fibrosis patients, which is particularly difficult to treat, causing chronic and eventually fatal infections. (utmb.edu)
  • Burkholderia cenocepacia is a Gram-negative opportunistic pathogen of patients with cystic fibrosis and chronic granulomatous disease. (qxmd.com)
  • Delayed association of the NADPH oxidase complex with macrophage vacuoles containing the opportunistic pathogen Burkholderia cenocepacia. (qxmd.com)
  • Focusing on a pathogen of the cystic fibrosis lung, Burkholderia cenocepacia, we sequenced clones and metagenomes to unravel the mutations and evolutionary forces responsible for adaptation and diversification of a single biofilm community during 1,050 generations of selection. (pnas.org)
  • In this environment, we studied adaptation by a soil isolate of Burkholderia cenocepacia , an opportunistic pathogen responsible for fatal biofilm-associated lung infections in CF patients ( 14 , 15 ). (pnas.org)
  • Burkholderia cenocepacia ist ein ubiquitäres Bakterium, das in der Rhizosphäre bestimmter Pflanzen wachstumsfördernd, bei Zwiebeln jedoch durch die Auslösung von Weichfäule pathogen wirkt. (tum.de)
  • We previously made a link in Burkholderia cenocepacia between PA degradation and virulence by showing that insertional mutagenesis of paaA and paaE genes, that encode part of a multicomponent oxidase of PA-CoA, results in PA-conditional growth and an attenuated killing phenotype in the Caenorhabditis elegans model of infection. (frontiersin.org)
  • Virulence of B. cenocepacia H111 against C. elegans is dependent on σ 54 . (asm.org)
  • We used the C. elegans model of infection to screen a mini-Tn 5 mutant library of B. cenocepacia H111 for attenuated virulence. (asm.org)
  • Loutet, SA & Valvano, M 2010, ' A Decade of Burkholderia cenocepacia Virulence Determinant Research ', Infection and Immunity , vol. 78, no. 10, pp. 4088-4100. (qub.ac.uk)
  • B. cenocepacia utilizes a mechanism of cell-cell communication called quorum sensing to control gene expression including genes involved in virulence. (biomedcentral.com)
  • Previous work demonstrated that B. cenocepacia phenylacetic acid (PA) catabolic genes are up-regulated during growth in SCFM and are required for full virulence in a Caenorhabditis elegans host model. (umanitoba.ca)
  • Interestingly, our results also showed the over-expression of flagellin and membrane efflux protein which are involved in the virulence of B. cenocepacia. (umanitoba.ca)
  • B. cenocepacia survives inside macrophages, and this survival depends on the secretion of virulence factors, among which two (metallo)proteases. (knowledgeforgrowth.be)
  • By creating isogenic PSCP mutants, we determined that PSCP is required for the maximal virulence of B. cenocepacia K56-2 in an experimental model of chronic lung infection. (ucalgary.ca)
  • AY278465 Burkholderia cenocepacia strain LMG 16656 outer membrane lipoprotein precursor (opcL) gene, partial cds. (atcc.org)
  • AF148556 Burkholderia cepacia genomovar III 16S ribosomal RNA gene, partial sequence. (atcc.org)
  • The B. cenocepacia cblBACDS operon encodes the structural and accessory proteins required for the assembly of cable pili, as well as a gene designated cblS , predicted to encode a hybrid sensor kinase protein of bacterial two-component signal transduction systems. (asm.org)
  • These findings are consistent with the proposed function of the cblSTR gene products as a multicomponent signal transduction pathway controlling the expression of cable pilus biosynthetic genes in B. cenocepacia . (asm.org)
  • Furthermore, insertional inactivation of the cblA gene in B. cenocepacia has been demonstrated to lead to a block in cable pilus biogenesis ( 40 ). (asm.org)
  • This study provides support to the notion that OAg LPS modifications are an important factor in the adaptation of B. cenocepacia to chronic infection and that the heterogeneity of OAgs relates to variation within the OAg gene cluster, indicating that the gene cluster might have been assembled through multiple horizontal transmission events. (frontiersin.org)
  • Using reporter activity assays, we demonstrate that 3-OHPA activated PA degradation gene promoters of Burkholderia cenocepacia K56-2 in a paaK1 -dependent manner, while 4-OHPA had no effect. (frontiersin.org)
  • We hypothesize that B. cenocepacia alters gene expression after internalization by macrophages, inducing genes involved in intracellular survival and host adaptation. (uwo.ca)
  • We examine differential gene expression by intracellular B. cenocepacia using selective capture of transcribed sequences (SCOTS) with both competitive enrichment and microarray analysis. (uwo.ca)
  • Global gene expression profiles during growth in stationary phase were generated using microarrays of B. cenocepacia cepR , cciR and cepRcciIR mutants. (biomedcentral.com)
  • For the first time, the influence of CciIR on global gene regulation in B. cenocepacia has been elucidated. (biomedcentral.com)
  • The 14 RND pumps gene we have identified in the genome of B. cenocepacia suggest that active efflux could be a major mechanism underlying antimicrobial resistance in this microorganism. (biomedcentral.com)
  • Transcriptomic profiling across nine growth conditions was performed to identify the global gene expression changes made when B. cenocepacia changes niches from an environmental lifestyle to infection. (cf.ac.uk)
  • In this study, we cloned and sequenced the zmpA gene encoding for PSCP, an extracellular metalloprotease of B. cenocepacia. (ucalgary.ca)
  • The gene is highly conserved and widespread in the Burkholderia cepacia complex. (pubfacts.com)
  • Here we constructed and characterized a B. cenocepacia tonB mutant strain, which was unable to actively transport iron, to test whether this single gene deletion mutant (strain renamed GAP001) protected against an acute respiratory B. cenocepacia lethal infection. (utmb.edu)
  • Dabei wurde die Bedeutung der QS-Gene cepR und rsuA für die Pathogenität von B. cenocepacia bestätigt. (tum.de)
  • Combined activity of Ugd(BCAL2946) and Ugd(BCAM0855) is essential for the survival of Burkholderia cenocepacia but only the most highly expressed ugd gene, Ugd(BCAL2946), is required for polymyxin B resistance. (brenda-enzymes.org)
  • We report a case of a vaginal infection caused by a strain of Burkholderia cenocepacia . (cdc.gov)
  • We report a case of vaginal infection, caused by B. cenocepacia, in a patient affected by smoldering myeloma, and chronic hepatitis C virus (HCV) infection. (cdc.gov)
  • In this work, we characterize later-stage infection of macrophages by B. cenocepacia , showing replication within an acidified endosomal compartment suggestive of a phagolysosome. (uwo.ca)
  • As a whole, secretion systems appear to increase pathogenicity in macrophages while limiting the spread of B. cenocepacia infection. (uwo.ca)
  • Members of the Burkholderia cepacia complex, normally present in soil microbiota, cause nosocomial lung infection especially in hospitalized cystic fibrosis patients. (microbiologyresearch.org)
  • IST439 was the first B. cenocepacia isolate recovered from the patient and it is thought to have initiated the infection while the IST4113 isolate was recovered three years later, after a period of exacerbated infection that compelled the patient to hospitalization and intravenous therapy with gentamicine and ceftazidime. (omicsdi.org)
  • Clinafloxacin for Treatment of Burkholderia cenocepacia Infection in a" by Akshu Balwan, David P Nicolau et al. (mainehealth.org)
  • Clinafloxacin for Treatment of Burkholderia cenocepacia Infection in a Cystic Fibrosis Patient. (mainehealth.org)
  • Respiratory infection with Burkholderia cenocepacia is associated with accelerated decline in lung function and increased mortality in cystic fibrosis (CF) patients (A. M. Jones, M. E. Dodd, J. R. W. Govan, V. Barcus, C. J. Doherty, J. Morris, and A. K. Webb, Thorax 59:948-951, 2004, http://dx.doi.org/10.1136/thx.2003.017210). (mainehealth.org)
  • B. cenocepacia often possesses innate resistance to multiple antimicrobial classes, making eradication uncommon in established infection (P. B. Davis, Am J Respir Crit Care Med 173:475-482, 2006, http://dx.doi.org/10.1164/rccm.200505-840OE). (mainehealth.org)
  • We report the use of clinafloxacin in a CF patient with advanced B. cenocepacia infection, present pharmacokinetic (PK) data, and discuss the potential therapeutic role of clinafloxacin in patients with this condition. (mainehealth.org)
  • Chronic infection with opportunistic pathogens including Burkholderia cepacia complex (Bcc) is a hallmark of cystic fibrosis (CF). We investigated the adaptive mechanisms facilitating chronic lung infection in sequential Bcc isolates from two siblings with CF (P1 and P2), one of whom also experienced intermittent blood-stream infections (P2). (maynoothuniversity.ie)
  • Burkholderia cenocepacia infection: disruption of phagocyte immune functions through Rho GTPase inactivation. (qxmd.com)
  • Burkholderia cenocepacia has been a very hard to treat bacterial infection for our Cystic Fibrosis community. (cysticfibrosis.com)
  • 2012) Modelling Co-Infection of the Cystic Fibrosis Lung by Pseudomonas aeruginosa and Burkholderia cenocepacia Reveals Influences on Biofilm Formation and Host Response. (plos.org)
  • A = averages) shows the log 2 fold changes in transcript expression in B. cenocepacia H111 grown under nitrogen-limited (S) versus nitrogen-replete (N) conditions. (asm.org)
  • Box plot for the numbers of reads per kilobase per million (RPKM) for genes in the wild-type strain B. cenocepacia H111, the σ 54 mutant (mt), and the complemented mutant (comp). (asm.org)
  • Cells of the wild-type strain B. cenocepacia H111 (B and F), the σ 54 mutant (C and G), and the complemented mutant (D and H) were grown on plates containing limiting amounts of ammonium. (asm.org)
  • C) Swimming motility of the wild-type strain B. cenocepacia H111, the σ 54 mutant, and the complemented mutant. (asm.org)
  • Using a discovery shotgun proteomics approach, the expressed proteome of Burkholderia cenocepacia (strain H111) was analyzed under normal and starved nitrogen growth conditions. (omicsdi.org)
  • In dieser Arbeit wurde der Nematode Caenorhabditis elegans als Modell zur Untersuchung der Pathogenität des klinischen Isolats B. cenocepacia H111 verwendet. (tum.de)
  • Durch den Einsatz spezifischer QS-Hemmstoffe konnte die Pathogenität von B. cenocepacia H111 und dem bei Patienten mit Mukoviszidose vorherrschenden Stamm, Pseudomonas aeruginosa, erheblich vermindert werden. (tum.de)
  • Time-kill analysis of a stationary phase culture of Burkholderia cenocepacia strain LMG 16656 when exposed to geraniol (0.25%, v/v) and 5 mM EDTA. (figshare.com)
  • Pseudomonas aeruginosa and Burkholderia cenocepacia are the causative agents of chronic airway infections and a major cause of mortality in cystic fibrosis patients. (ucalgary.ca)
  • Extracellular metalloproteases of pseudomonas aeruginosa and burkholderia cenocepacia as potential immunotherapeutic targets (Unpublished doctoral thesis). (ucalgary.ca)
  • Formerly known as Pseudomonas cepacia, the bacterium now known as Burkholderia cepacia, was first described in 1950 as the cause of sour skin of onions by Cornell University plant pathologist Walter Burkholder (1). (apsnet.org)
  • These mutations in turn were surprisingly well represented among mutations that evolved in cystic fibrosis isolates of both Burkholderia and Pseudomonas . (pnas.org)
  • My group has studied the pathogenesis and ecology of bacterial opportunistic pathogens, ranging from Pseudomonas aeruginosa to mycobacteria, with a major focus on Burkholderia cepacia complex bacteria. (cardiff.ac.uk)
  • Pseudomonas and Burkholderia bacteria cause devastating infections in people with cystic fibrosis and we have used molecular biology and genomic approaches to track their ability to spread between patients, resist killing by antibiotics and cause lung disease. (cardiff.ac.uk)
  • This is where I began to developing expertise in the cystic fibrosis microbiology, working on Pseudomonas aeruginosa and Burkholderia cepacia complex bacteria that cause devastating lung infections in these individuals. (cardiff.ac.uk)
  • When the authors grew the more-resistant B. cenocepacia with another strain of bacteria called Pseudomonas aeruginosa (a disease-causing bacterium that can co-exist with B. cenocepacia ), the P. aeruginosa were much more resistant to the antibiotic than when they grew in isolation. (plos.org)
  • Burkholderia cenocepacia belongs to a group of closely related organisms called the B. cepacia complex (Bcc) which are important opportunistic human pathogens. (biomedcentral.com)
  • Burkholderia cenocepacia is a Gram-negative aerobic bacterium that belongs to a group of opportunistic pathogens displaying diverse environmental and pathogenic lifestyles. (cf.ac.uk)
  • These infections are caused, among others, by opportunistic pathogens, for example Burkholderia cenocepacia(B. cenocepacia). (knowledgeforgrowth.be)
  • Here, we will discuss six zebrafish models for important human pathogens ( Mycobacterium , Salmonella , Burkholderia , Staphylococcus , Shigella and Candida ), emphasizing the novel insights that these models have recently provided into macrophage biology and highlighting how this could lead to the finding of new host-derived therapeutic strategies. (biologists.org)
  • As B. cenocepacia and C. albicans are frequently encountered human pathogens, identification of the BDSF signal and its activity thus provides a new insight into the molecular grounds of their antagonistic interactions whose importance to microbial ecology and pathogenesis is now becoming evident. (pubfacts.com)
  • The Burkholderia cepacia complex ( Bcc ) bacteria are opportunistic pathogens which cause nosocomial infections and are especially dangerous for cystic fibrosis (CF) patients. (hindawi.com)
  • Organisms of the Burkholderia cepacia complex are especially important pathogens in cystic fibrosis (CF), with a propensity for patient-to-patient spread and long-term respiratory colonization. (cdc.gov)
  • In the last few years, we made a very exciting discovery that the Burkholderia bacteria I had studied as pathogens, also make some very potent antibiotics which kill other multidrug resistant bacteria and fungi. (cardiff.ac.uk)
  • DNA-DNA hybridisation experiments between isolates representing Burkholderia cepacia genomovar III recA lineages IIIA and IIIB reinforced the classification of both phylogenetic subgroups as a single genospecies, distinct from B. cepacia (genomovar I). A formal classification of B. cepacia genomovar III encompassing the recA lineages IIIA and IIIB, and the new recA lineages IIIC and IIID, as B. cenocepacia sp. (nih.gov)
  • b Includes (number of isolates) Burkholderia contaminans (11), Burkholderia dolosa (10), Burkholderia ambifaria (9), and Burkholderia stabilis (9). (asm.org)
  • The Burkholderia cepacia complex (Bcc) is a group of genetically related environmental bacteria that can cause chronic opportunistic infections in patients with cystic fibrosis (CF) and other underlying diseases. (qub.ac.uk)
  • Burkholderia cenocepacia is a member of the B. cepacia complex (Bcc), a group of opportunistic bacteria that infect the airways of patients with cystic fibrosis (CF) and are extraordinarily resistant to almost all clinically useful antibiotics. (microbiologyresearch.org)
  • Burkholderia cenocepacia infects patients with cystic fibrosis. (microbiologyresearch.org)
  • Burkholderia cenocepacia can cause serious infections and epidemics in patients with cystic fibrosis (CF). A CF population in the Czech Republic experienced an epidemic outbreak caused by a B. cenocepacia ST-32 strain. (plymouth.ac.uk)
  • The Burkholderia cepacia complex (Bcc) is a large and diverse group of related gram-negative bacteria, which inhabit a wide range of environmental niches, including freshwater and soil. (asm.org)
  • For example, our recent discovery that Burkholderia bacteria can produce novel polyketide antibiotics that target multidrug resistant bacterial infections is very exciting. (cardiff.ac.uk)
  • We are pleased to announced the forthcoming publication of a second book on Burkholderia bacteria: "Burkholderia: from genomes to function" which can be previewed at Horizon Press ( http://www.horizonpress.com/burk2 ). (cardiff.ac.uk)
  • While Burkholderia bacteria and cystic fibrosis microbiology remain major research foci, my interests are still wide and I am always getting involved in lots of very interesting molecular microbiology projects. (cardiff.ac.uk)
  • A novel sensor kinase-response regulator hybrid controls biofilm formation and type VI secretion system activity in Burkholderia cenocepacia. (burkholderia.com)
  • Here, we show that intracellular B. cenocepacia expressing a type VI secretion system (T6SS) affects the activation of the Rac1 and Cdc42 RhoGTPase by reducing the cellular pool of GTP-bound Rac1 and Cdc42. (qxmd.com)
  • The characterization of antibiotic efflux pumps in B. cenocepacia is an obligatory step prior to the design of specific, potent bacterial inhibitors for the improved control of infectious diseases. (biomedcentral.com)
  • We report here the isolation and characterization of a novel signaling molecule, cis-2-dodecenoic acid (BDSF), from Burkholderia cenocepacia. (pubfacts.com)
  • Characterization of rhamnolipid production by Burkholderia glumae. (springer.com)
  • We sequenced the draft genome of Burkholderia cenocepacia KC-01, isolated from the coastal saline soil, and identified several potential sRNAs in silico . (microbiologyresearch.org)
  • The OAg repeating unit of the LPS from IST439, the initial isolate, is a [→2)-β-D-Rib f -(1→4)-α-D-Gal p NAc-(1→] disaccharide, which was not previously described in B. cenocepacia . (frontiersin.org)
  • In particular, the lack of efficacious antimicrobial treatments for infections caused by the Burkholderia cepaci a complex is causing high morbidity and mortality of cystic fibrosis patients and of patients with nosocomial infections. (asm.org)
  • Synthetic cystic fibrosis sputum medium (SCFM) is rich in amino acids and supports robust growth of Burkholderia cenocepacia, a member of the Burkholderia cepacia complex (Bcc). (umanitoba.ca)
  • Results: Efficient recombinant protein production and crystallization protocols were developed, and high-resolution crystal structures of IspF from P. falciparum ( Pf IspF) and B. cenocepacia ( Bc IspF) in complex with cytidine nucleotides determined. (edu.pl)
  • Whether Burkholderia cepacia complex should be an objectionable organism in antiseptic solutions with acceptable total bacterial counts is controversial. (cdc.gov)
  • The bacterial factors involved in B. cenocepacia intracellular survival are not fully elucidated. (microbiologyresearch.org)
  • We specifically demonstrate that undefined rich media leads to higher susceptibility of B. cenocepacia to P. aeruginosa toxins like cyanide as compared to a synthetic medium (SCFM), that mimics the CF lung nutritional content. (dartmouth.edu)
  • In the early 1980s Burkholderia cepaciabecame associated with epidemics of severe lung infections ('cepacia syndrome') traced, in many cases, to CF treatment centers and social gatherings where CF patients apparently acquired the disease from other B. cepacia-infected individuals (19). (apsnet.org)
  • Cable pili have been proposed to facilitate binding to respiratory epithelia and mucin ( 29 , 31 ) and may also play a role in mediating B. cenocepacia cell-cell interactions ( 40 ). (asm.org)
  • Burkholderia cenocepacia is notorious for causing respiratory tract infections in people with cystic fibrosis. (asm.org)
  • These studies provide the initial steps toward producing anti-peptide 15 immunotherapeutic agents for use in P. aeruginosa or B. cenocepacia respiratory infections. (ucalgary.ca)
  • Infections with B. cenocepacia are particularly difficult to treat due to their high level of resistance against a wide range of antimicrobial agents ( 1 , 3 , 4 ). (asm.org)
  • Its overexpression in E. coli conferred resistance to several antibiotics and to ethidium bromide but it remains to be determined if this pump play a significant role in the antimicrobial intrinsic resistance of B. cenocepacia . (biomedcentral.com)
  • Oxidative stress of Burkholderia cenocepacia induces insertion sequence-mediated genomic rearrangements that interfere with macrorestriction-based genotyping. (plymouth.ac.uk)
  • Burkholderia cenocepacia induces neutrophil necrosis in chronic granulomatous disease. (medscape.com)
  • In this work, we aimed to determine the relevance of PaaK1 activity to the killing ability of B. cenocepacia to C. elegans . (frontiersin.org)
  • Um Virulenzfaktoren zu identifizieren, die nicht durch das QS-System reguliert sind, wurde die Pathogenität von ca. 1.000 B. cenocepacia Tn5-Insertionsmutanten im C. elegans Modell untersucht. (tum.de)
  • Insgesamt wurde gezeigt, dass einzelne Virulenzfaktoren von B. cenocepacia in C. elegans keine (Protease- und Lipase-Aktivität sowie EPS-Produktion) oder nur eine geringe Rolle (Siderophore) spielen und dass die Pathogenität dieses Bakteriums durch ein komplexes Zusammenwirken verschiedener Faktoren und Mechanismen reguliert ist, die erst zum Teil aufgeklärt sind. (tum.de)
  • We find that B. cenocepacia has low genome-wide mutation rates with insertion-deletion mutations biased toward deletions, consistent with the idea that deletion pressure reduces prokaryotic genome sizes. (genetics.org)
  • Unlike prior studies of other organisms, mutations in B. cenocepacia are not AT biased, which suggests that at least some genomes with high %GC content experience unusual base-substitution mutation pressure. (genetics.org)
  • A hallmark of B. cenocepacia infections is the inability to eradicate the organism because of multiple intrinsic antibiotic resistance. (biomedcentral.com)
  • Complete Genome Sequence of Burkholderia cenocepacia Phage Magia. (pubfacts.com)
  • Complete Genome Sequence of Burkholderia cenocepacia Phage Mica. (pubfacts.com)
  • RT "Complete sequence of chromosome 2 of Burkholderia cenocepacia MC0- RT 3. (univ-lyon1.fr)
  • Furthermore, proteomic analysis utilizing iTRAQ were used to study the protein expression of B. cenocepacia K56-2 grown in the amino acid-rich SCFM. (umanitoba.ca)
  • A glycoengineered antigen exploiting a conserved protein O-glycosylation pathway in the Burkholderia genus for detection of glanders infections. (pubfacts.com)
  • Attachment was dramatically reduced in a B. cenocepacia K56-2Δlxa-locus deletion mutant, further indicating that it encodes protein(s) involved in host-cell attachment. (maynoothuniversity.ie)
  • The distribution of internal transcription start sites can be used to identify genomic islands in B. cenocepacia. (nih.gov)
  • Deletion of the lxa locus resulted in B. cenocepacia mutants with aerobic growth deficiencies in minimal medium and compromised viability after prolonged incubation in the absence of oxygen. (cf.ac.uk)
  • In summary, transcriptomic profiling of B. cenocepacia revealed an unexpected ability of aerobic Burkholderia to persist in the absence of oxygen and identified the novel lxa locus as key determinant of this important ecophysiological trait. (cf.ac.uk)
  • Infections with B. cenocepacia are very difficult to treat due to their high intrinsic resistance to most antibiotics. (nih.gov)
  • Multidrug resistance in B. cenocepacia can be ascribed to different mechanisms, including the activity of efflux pumps and biofilm formation. (asm.org)
  • Recently, we showed that the RND-4 efflux system is involved in the resistance to a new thiopyridine drug effective against B. cenocepacia ( 11 ). (asm.org)
  • B. cenocepacia is intrinsically resistant to antibiotic therapy and often impossible to eradicate from lungs of infected CF patients [ 9 ]. (biomedcentral.com)
  • The authors suspected that the B. cenocepacia were releasing something into their environment that interfered with the action of the antibiotic, making it less potent. (plos.org)
  • A common theme emerging from these studies is that B. cenocepacia establishes chronic infections in immuno-compromised patients, which depend more on determinants mediating host niche adaptation than those involved directly in host cells and tissue damage. (qub.ac.uk)
  • In addition, we reported on the identification of lifestyle-specific efflux pumps involved in B. cenocepacia tolerance to the disinfectant chlorhexidine (CHX) ( 12 ). (asm.org)
  • Despite advances in anti-microbial therapy, the treatment of infections caused by P. aeruginosa and B. cenocepacia remains a challenge. (ucalgary.ca)
  • Overall, our study shows that the polymicrobial environment can have profound effects on negative interactions mediated by P aeruginosa against B. cenocepacia. (dartmouth.edu)
  • Why might the P. aeruginosa be more resistant when they were in the presence of B. cenocepacia ? (plos.org)