Burkholderia cenocepacia
Burkholderia
A genus of gram-negative, aerobic, rod-shaped bacteria. Organisms in this genus had originally been classified as members of the PSEUDOMONAS genus but overwhelming biochemical and chemical findings indicated the need to separate them from other Pseudomonas species, and hence, this new genus was created.
Burkholderia cepacia complex
Burkholderia cepacia
Burkholderia pseudomallei
Quorum Sensing
Cystic Fibrosis
An autosomal recessive genetic disease of the EXOCRINE GLANDS. It is caused by mutations in the gene encoding the CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR expressed in several organs including the LUNG, the PANCREAS, the BILIARY SYSTEM, and the SWEAT GLANDS. Cystic fibrosis is characterized by epithelial secretory dysfunction associated with ductal obstruction resulting in AIRWAY OBSTRUCTION; chronic RESPIRATORY INFECTIONS; PANCREATIC INSUFFICIENCY; maldigestion; salt depletion; and HEAT PROSTRATION.
Melioidosis
Gene Expression Regulation, Bacterial
Biofilms
Encrustations, formed from microbes (bacteria, algae, fungi, plankton, or protozoa) embedding in extracellular polymers, that adhere to surfaces such as teeth (DENTAL DEPOSITS); PROSTHESES AND IMPLANTS; and catheters. Biofilms are prevented from forming by treating surfaces with DENTIFRICES; DISINFECTANTS; ANTI-INFECTIVE AGENTS; and antifouling agents.
Rhizoctonia
Virulence
Microbial Viability
Mutagenesis, Insertional
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
Burkholderia gladioli
Bronchopneumonia
Polymyxin B
4-Butyrolactone
Glanders
Drug Resistance, Bacterial
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Virulence Factors
Those components of an organism that determine its capacity to cause disease but are not required for its viability per se. Two classes have been characterized: TOXINS, BIOLOGICAL and surface adhesion molecules that effect the ability of the microorganism to invade and colonize a host. (From Davis et al., Microbiology, 4th ed. p486)
Genetic Complementation Test
Phagosomes
Bacterial Typing Techniques
Sequence Analysis, DNA
Mutation
DNA Transposable Elements
Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
Microscopy, Electron, Transmission
Sputum
Operon
Glycolysis
A metabolic process that converts GLUCOSE into two molecules of PYRUVIC ACID through a series of enzymatic reactions. Energy generated by this process is conserved in two molecules of ATP. Glycolysis is the universal catabolic pathway for glucose, free glucose, or glucose derived from complex CARBOHYDRATES, such as GLYCOGEN and STARCH.
Gluconeogenesis
Methane
Mesna
Methanosarcina
A genus of anaerobic, irregular spheroid-shaped METHANOSARCINALES whose organisms are nonmotile. Endospores are not formed. These archaea derive energy via formation of methane from acetate, methanol, mono-, di-, and trimethylamine, and possibly, carbon monoxide. Organisms are isolated from freshwater and marine environments.
Gene Expression Profiling
Urease
Urea
Microscopy, Atomic Force
A type of scanning probe microscopy in which a probe systematically rides across the surface of a sample being scanned in a raster pattern. The vertical position is recorded as a spring attached to the probe rises and falls in response to peaks and valleys on the surface. These deflections produce a topographic map of the sample.
Adhesins, Bacterial
Cell-surface components or appendages of bacteria that facilitate adhesion (BACTERIAL ADHESION) to other cells or to inanimate surfaces. Most fimbriae (FIMBRIAE, BACTERIAL) of gram-negative bacteria function as adhesins, but in many cases it is a minor subunit protein at the tip of the fimbriae that is the actual adhesin. In gram-positive bacteria, a protein or polysaccharide surface layer serves as the specific adhesin. What is sometimes called polymeric adhesin (BIOFILMS) is distinct from protein adhesin.
BcsKC is an essential protein for the type VI secretion system activity in Burkholderia cenocepacia that forms an outer membrane complex with BcsLB. (1/74)
(+info)Ecological succession in long-term experimentally evolved biofilms produces synergistic communities. (2/74)
(+info)Inactivation of macrophage Rab7 by Burkholderia cenocepacia. (3/74)
(+info)The pobA gene of Burkholderia cenocepacia encodes a group I Sfp-type phosphopantetheinyltransferase required for biosynthesis of the siderophores ornibactin and pyochelin. (4/74)
(+info)The Burkholderia cenocepacia LysR-type transcriptional regulator ShvR influences expression of quorum-sensing, protease, type II secretion, and afc genes. (5/74)
(+info)Virulence of an emerging respiratory pathogen, genus Pandoraea, in vivo and its interactions with lung epithelial cells. (6/74)
(+info)Burkholderia cenocepacia O polysaccharide chain contributes to caspase-1-dependent IL-1beta production in macrophages. (7/74)
(+info)CD4+ T-cell immunity to the Burkholderia pseudomallei ABC transporter LolC in melioidosis. (8/74)
(+info)
Disruption of Tight Junctions during Traversal of the Respiratory Epithelium by Burkholderia cenocepacia | Infection and...
Characterization of BCAM0224, a Multifunctional Trimeric Autotransporter from the Human Pathogen Burkholderia cenocepacia |...
A Decade of Burkholderia cenocepacia Virulence Determinant Research<...
Genome-wide transcription start site profiling in biofilm-grown Burkholderia cenocepacia J2315
Characterization of the cciIR Quorum-Sensing System in Burkholderia cenocepacia | Infection and Immunity
Burkholderia cenocepacia ATCC ® BAA-245D-5™
KEGG PATHWAY: Methane metabolism - Burkholderia cenocepacia J2315
KEGG PATHWAY: Glycolysis / Gluconeogenesis - Burkholderia cenocepacia J2315
The unexpected discovery of a novel low-oxygen-activated locus for the anoxic persistence of Burkholderia cenocepacia -ORCA
The intracellular behaviour of Burkholderia cenocepacia in murine macrophages by Jennifer S. Tolman
Overview: bceU, Burkholderia cenocepacia H111
Down the sink: the local experience of a Burkholderia cenocepacia outbreak at the Canberra Hospital - 2021 ACIPC Conference
Recombinant Burkholderia cenocepacia ATP-dependent Clp protease adapter protein ClpS(clpS) - Cusabio
Predicted Orthologs - RBBH
bcscV - Type III secretion system protein - Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 ...
xseB - Exodeoxyribonuclease 7 small subunit - Burkholderia cenocepacia (strain HI2424) - xseB gene & protein
Scientists Identify Key Target to Kill Cystic Fibrosis Bug - BioTech Weblog
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Frontiers | 3-Hydroxyphenylacetic Acid Induces the Burkholderia cenocepacia Phenylacetic Acid Degradation Pathway - Toward...
Microbiology Society Journals | An electron transfer flavoprotein is essential for viability and its depletion causes a rod-to...
Loss ofO-linked protein glycosylation inBurkholderia cenocepaciaimpairs biofilm formation and siderophore production via...
Synthesis and evaluation of the quorum sensing inhibitory effect of substituted triazolyldihydrofuranones - Institutional...
A comparison of alternative 60-mer probe designs in an in-situ synthesized oligonucleotide microarray | BMC Genomics | Full Text
Survival curves for D. melanogaster infected with B. ce | Open-i
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Comparison of biofilm formation between Burkholderia multivorans Burkholderia cenocepacia Pseudomonas aeruginosa in the...
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Updated Version of the Burkholderia cepacia Complex Experimental Strain Panel | Journal of Clinical Microbiology
Distribution and genomic location of active insertion sequences in the Burkholderia cepacia complex | Microbiology Society
A General Protein O-glycosylation System Within the Burkholderia Cepacia Complex Is Involved in Motility and Virulence - PubMed
Specific and sensitive plate assay for bacterial lipases. | Applied and Environmental Microbiology
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Toxic Small RNA
Sass AM, De Waele S, Daled S, Devreese B, Deforce D, Van Nieuwerburgh F, Coenye T (October 2019). "Burkholderia cenocepacia ... Sass A, Kiekens S, Coenye T (November 2017). "Identification of small RNAs abundant in Burkholderia cenocepacia biofilms reveal ... Characterization of the secondary structures of 4 tsRNAs in Burkholderia cenocepacia suggests they form double hairpin ... Typically, each species of Burkholderia has 3-5 homologous tsRNAs. Experiments with four species of the Burkholderia lineage ...
Ornibactin
... is produced by Burkholderia cenocepacia under iron-deficient conditions. B. cenocepacia is known to ... "The ornibactin biosynthesis and transport genes of Burkholderia cenocepacia are regulated by an extracytoplasmic function sigma ... "Ornibactin production and transport properties in strains of Burkholderia vietnamiensis and Burkholderia cepacia (formerly ... Coenye T, Vandamme P, Govan JR, LiPuma JJ (October 2001). "Taxonomy and identification of the Burkholderia cepacia complex". ...
Pathogenic bacteria
Examples of these opportunistic pathogens include Pseudomonas aeruginosa, Burkholderia cenocepacia, and Mycobacterium avium. ...
Hopanoids
... and motility in Burkholderia cenocepacia". Journal of Bacteriology. 193 (23): 6712-23. doi:10.1128/JB.05979-11. PMC 3232912. ... "Elucidation of the Burkholderia cenocepacia hopanoid biosynthesis pathway uncovers functions for conserved proteins in hopanoid ...
Prabhu B. Patil
"Genomics Reveals a Unique Clone of Burkholderia cenocepacia Harboring an Actively Excising Novel Genomic Island". Frontiers in ...
Innate immune system
... biofilms are present in the chronic Pseudomonas aeruginosa and Burkholderia cenocepacia infections characteristic of cystic ...
H2cR sRNA
"The Novel Cis-Encoded Small RNA h2cR Is a Negative Regulator of hfq2 in Burkholderia cenocepacia". PLoS ONE. 7 (10): e47896. ... In molecular biology, the h2cR sRNA is a small RNA produced by species of the bacterial genus Burkholderia. It binds to the ...
Social motility
As first discovered in plants, diffusible signal factors (DSFs) have been found in bacteria such as Burkholderia cenocepacia ...
Immune system
... such as the chronic Pseudomonas aeruginosa and Burkholderia cenocepacia infections characteristic of cystic fibrosis. Other ...
O-linked glycosylation
... archaea and a number of pathogenic bacteria including Burkholderia cenocepacia, Neisseria gonorrhoeae and Acinetobacter ... "A general protein O-glycosylation system within the Burkholderia cepacia complex is involved in motility and virulence". ...
Bacteria
Finally, some species, such as Pseudomonas aeruginosa, Burkholderia cenocepacia, and Mycobacterium avium, are opportunistic ...
Phosphate solubilizing bacteria
... cultures were identified as Burkholderia cenocepacia strain VIMP01 (JQ867371), Burkholderia gladioli strain VIMP02 (JQ811557), ... Burkholderia gladioli strain VIMP03 (JQ867372) and Burkholderia species strain VIMP04 (JQ867373)6]. Additionally, phosphate (P ... Isolates were strains of Burkholderia named as VIMP01, VIMP02, VIMP03 and VIMP04. VIMP (Vasantdada Sugar Institute Isolate by ...
Mallory Smith
By age 15, bacteria had colonized Smith's lungs, including a form of Burkholderia cenocepacia which mutated over a decade of ...
Burkholderia cenocepacia
"Burkholderia cenocepacia". NCBI Taxonomy Browser. 95486. Type strain of Burkholderia cenocepacia at BacDive - the Bacterial ... is Burkholderia cenocepacia, a member of a bacterial group collectively referred to as the Burkholderia cepacia complex (Bcc ... Burkholderia cenocepacia is a species of Gram-negative bacteria that is common in the environment, can form a biofilm with ... sRNAs identified in Burkholderia cenocepacia KC-0 were upregulated under iron depletion and oxidate stress. In Seattle, a team ...
Burkholderia cepacia complex
May 2007). "A Putative Gene Cluster for Aminoarabinose Biosynthesis Is Essential for Burkholderia cenocepacia Viability". J. ... Loutet SA, Valvano MA (October 2010). "A decade of Burkholderia cenocepacia virulence determinant research". Infect. Immun. 78 ... Burkholderia cepacia complex in the NCBI Taxonomy Browser Type strain of Burkholderia cepacia complex at BacDive, the Bacterial ... Burkholderia cepacia complex (BCC), or simply Burkholderia cepacia, is a group of catalase-producing, lactose-nonfermenting, ...
Burkholderia
... alpina Burkholderia ambifaria Burkholderia anthina Burkholderia arboris Burkholderia cenocepacia Burkholderia ... Burkholderia dolosa Burkholderia gladioli Burkholderia glumae Burkholderia humptydooensis Burkholderia lata Burkholderia latens ... Burkholderia mallei Burkholderia metallica Burkholderia multivorans Burkholderia oklahomensis Burkholderia plantarii ... Burkholderia pseudomallei Burkholderia pseudomultivorans Burkholderia puraquae Burkholderia pyrrocinia Burkholderia seminalis ...
Pathema
Burkholderia mallei Burkholderia pseudomallei Burkholderia thailandensis Burkholderia ambifaria Burkholderia cenocepacia ... Burkholderia multivorans Burkholderia vietnamiensis Burkholderia xenovorans Burkholderia sp. Burkholderia phages Pseudomonas ... Burkholderia mallei, Burkholderia pseudomallei, Clostridium perfringens, and Entamoeba histolytica) involved in new and re- ... Bacillus anthracis Bacillus cereus Bacillus thuringiensis Bacillus subtilis Bacillus pumilus Pathema-Burkholderia] ...
Bakteria, kamusi elezo huru
Burkholderia cenocepacia, na Mycobacterium avium ni ni pathojeni ambazo hutaka kutumia hali fulani ya binadamu walio na uhaba ...
Kategori:Semua regnum bacteria bahasa Indonesia, ensiklopedia bebas
Burkholderia cenocepacia. *Burkholderia gladioli. C. *Campylobacter. *Chlamydia trachomatis. *Chlamydiae. *Chlorobi. * ...
Sistem imun bahasa Indonesia, ensiklopedia bebas
Biofilm dapat ditemui pada banyak infeksi, seperti infeksi Pseudomonas aeruginosa kronis dan Burkholderia cenocepacia, yang ...
Imunski sistem - Wikipedija, prosta enciklopedija
Nekatere bakterije (uspešne trajne okužbe - kronična Pseudomonas Aeruginosa in Burkholderia cenocepacia, značilne za cistično ...
Burkholderia cenocepacia - Wikipedia
"Burkholderia cenocepacia". NCBI Taxonomy Browser. 95486. Type strain of Burkholderia cenocepacia at BacDive - the Bacterial ... is Burkholderia cenocepacia, a member of a bacterial group collectively referred to as the Burkholderia cepacia complex (Bcc ... Burkholderia cenocepacia is a species of Gram-negative bacteria that is common in the environment, can form a biofilm with ... sRNAs identified in Burkholderia cenocepacia KC-0 were upregulated under iron depletion and oxidate stress. In Seattle, a team ...
Burkholderia cenocepacia ATCC ® BAA-245D-5™
Genomic DNA from Burkholderia cenocepacia strain LMG 16656 TypeStrain=False Application: ... Burkholderia cenocepacia (ATCC® BAA-245D-5™) Strain Designations: Genomic DNA from Burkholderia cenocepacia strain LMG 16656 [ ... Burkholderia cepacia (Palleroni and Holmes) Yabuuchi et al. Strain Designations Genomic DNA from Burkholderia cenocepacia ... Burkholderia cenocepacia ATCC® BAA-245D-5™ dried Total DNA: At least 5 µg in 1X TE buffer ...
RCSB PDB - 5IXG: Crystal Structure of Burkholderia cenocepacia BcnB
Here we show that a soluble bacterial lipocalin produced by Burkholderia cenocepacia upon exposure to sublethal antibiotic ... Burkholderia cenocepacia PC184. Mutation(s): 0 Gene Names: BCPG_01022. Membrane Entity: Yes ... Here we show that a soluble bacterial lipocalin produced by Burkholderia cenocepacia upon exposure to sublethal antibiotic ... cenocepacia of lipocalin genes from Pseudomonas aeruginosa , Mycobacterium tuberculosis , and methicillin-resistant ...
Burkholderia cenocepacia sp. nov.--a new twist to an old story. - PubMed - NCBI
Burkholderia cenocepacia sp. nov.--a new twist to an old story.. Vandamme P1, Holmes B, Coenye T, Goris J, Mahenthiralingam E, ... DNA-DNA hybridisation experiments between isolates representing Burkholderia cepacia genomovar III recA lineages IIIA and IIIB ... cenocepacia sp. nov., with LMG 16656 as the type strain, is proposed. ...
KEGG PATHWAY: Glycolysis / Gluconeogenesis - Burkholderia cenocepacia J2315
KEGG PATHWAY: Methane metabolism - Burkholderia cenocepacia J2315
Genome-wide transcription start site profiling in biofilm-grown Burkholderia cenocepacia J2315
Genes and novel regulatory RNAs putatively involved in B. cenocepacia biofilm formation were identified. These findings will ... help in understanding regulation of B. cenocepacia biofi … ... cenocepacia genome added relevant information to the J2315 ... Genome-wide transcription start site profiling in biofilm-grown Burkholderia cenocepacia J2315 BMC Genomics. 2015 Oct 13;16:775 ... Background: Burkholderia cenocepacia is a soil-dwelling Gram-negative Betaproteobacterium with an important role as ...
argG - Argininosuccinate synthase - Burkholderia cenocepacia (strain HI2424) - argG gene & protein
sp,A0AYH4,ASSY_BURCH Argininosuccinate synthase OS=Burkholderia cenocepacia (strain HI2424) OX=331272 GN=argG PE=3 SV=1 ... Burkholderia cenocepacia (strain HI2424). ,p>This subsection of the ,a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy ... cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › ...
ureA - Urease subunit gamma - Burkholderia cenocepacia (strain HI2424) - ureA gene & protein
sp,A0K578,URE3_BURCH Urease subunit gamma OS=Burkholderia cenocepacia (strain HI2424) OX=331272 GN=ureA PE=3 SV=1 ... Burkholderia cenocepacia (strain HI2424). ,p>This subsection of the ,a href="http://www.uniprot.org/help/names_and_taxonomy_ ... cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › ...
RCSB PDB - 5B57: Inward-facing conformation of ABC heme importer BhuUV from Burkholderia cenocepacia
Burkholderia cenocepacia J2315. Mutation(s): 0 Gene Names: hmuV, BCAM2630. EC: 3.6.3 (PDB Primary Data), 7.6.2 (UniProt). ... Burkholderia cenocepacia J2315. Mutation(s): 0 Gene Names: hmuU, BCAM2629. Membrane protein. Mpstruc. Group: TRANSMEMBRANE ... Find proteins for B4EKB4 (Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610)) ... Find proteins for B4EKB5 (Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610)) ...
JCI -
Salicylate induces an antibiotic efflux pump in Burkholderia cepacia complex genomovar III (B. cenocepacia)
Salicylate induces an antibiotic efflux pump in Burkholderia cepacia complex genomovar III (B. cenocepacia). ... Salicylate induces an antibiotic efflux pump in Burkholderia cepacia complex genomovar III (B. cenocepacia). ... and ciprofloxacin has been identified in Burkholderia cenocepacia (genomovar III), an important cystic fibrosis pathogen. Five ... These results suggest that salicylate is a natural substrate for the efflux pump in B. cenocepacia and imply that the ...
The Rate and Molecular Spectrum of Spontaneous Mutations in the GC-Rich Multichromosome Genome of Burkholderia cenocepacia |...
Burkholderia cenocepacia is a member of the B. cepacia complex, a diverse group of bacteria with important clinical ... J. J. LiPuma, and P. Vandamme, 2004 Recovery of Burkholderia cenocepacia strain PHDC from cystic fibrosis patients in Europe. ... The Rate and Molecular Spectrum of Spontaneous Mutations in the GC-Rich Multichromosome Genome of Burkholderia cenocepacia. ... The Rate and Molecular Spectrum of Spontaneous Mutations in the GC-Rich Multichromosome Genome of Burkholderia cenocepacia. ...
Characterization of BCAM0224, a Multifunctional Trimeric Autotransporter from the Human Pathogen Burkholderia cenocepacia |...
Burkholderia cenocepacia in cystic fibrosis: epidemiology and molecular mechanisms of virulence. Clin. Microbiol. Infect. 16: ... Genome-wide analysis of DNA repeats in Burkholderia cenocepacia J2315 identifies a novel adhesin-like gene unique to epidemic- ... Characterization of BCAM0224, a Multifunctional Trimeric Autotransporter from the Human Pathogen Burkholderia cenocepacia. ... Burkholderia cenocepacia creates an intramacrophage replication niche in zebrafish embryos, followed by bacterial dissemination ...
The Genome of Burkholderia cenocepacia J2315, an Epidemic Pathogen of Cystic Fibrosis Patients | Journal of Bacteriology
Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov., ... Burkholderia cenocepacia and Burkholderia multivorans: influence on survival in cystic fibrosis. Thorax 59:948-951. ... CDSs with matches identified by reciprocal FASTA with other Burkholderia species-B. cenocepacia HI2424, B. cenocepacia AU1054, ... cenocepacia island (CCI), are more frequently associated with B. cenocepacia IIIA strains than other B. cenocepacia recA ...
Candidate Essential Genes in Burkholderia cenocepacia J2315 Identified by Genome-Wide TraDIS
Burkholderia cenocepacia infection often leads to fatal cepacia syndrome in cystic fibrosis patients. However, antibiotic ... Candidate Essential Genes in Burkholderia cenocepacia J2315 Identified by Genome-Wide TraDIS Front Microbiol. 2016 Aug 22;7: ... Burkholderia cenocepacia infection often leads to fatal cepacia syndrome in cystic fibrosis patients. However, antibiotic ... cenocepacia growth under nutrient-depleted conditions. The library of B. cenocepacia mutants can subsequently be subjected to ...
Burkholderia cenocepacia Vaginal Infection in Patient with Smoldering Myeloma and Chronic Hepatitis C - Volume 10, Number 11...
Treatment with piperacillin/tazobactam eliminated B. cenocepacia infection and vaginal symptoms ... We report a case of a vaginal infection caused by a strain of Burkholderia cenocepacia. The strain was isolated from vaginal ... Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, Burkholderia cepacia genomovars I and III. J Clin ... Burkholderia anthina sp. nov. and Burkholderia pyrrocinia, two additional Burkholderia cepacia complex bacteria, may confound ...
KEGG GENOME: Burkholderia cenocepacia DDS 22E-1
Burkholderia cenocepacia epitopes - Immune Epitope Database (IEDB)
Frontiers | Structure of O-Antigen and Hybrid Biosynthetic Locus in Burkholderia cenocepacia Clonal Variants Recovered from a...
In this work, we investigated the lipopolysaccharide (LPS) of clinical variants of B. cenocepacia that were collected from a ... In this work, we investigated the lipopolysaccharide (LPS) of clinical variants of B. cenocepacia that were collected from a ... cenocepacia. The IST439 OAg biosynthetic gene cluster contains 7 of 23 genes that are closely homologous to genes found in B. ... cenocepacia. The IST439 OAg biosynthetic gene cluster contains 7 of 23 genes that are closely homologous to genes found in B. ...
Frontiers | 3-Hydroxyphenylacetic Acid Induces the Burkholderia cenocepacia Phenylacetic Acid Degradation Pathway - Toward...
We previously made a link in Burkholderia cenocepacia between phenylacetic acid (PA) degradation and virulence by showing that ... induces the phenylacetic acid degradative pathway in Burkholderia cenocepacia K56-2. Using reporter activity assays, we show ... induces the phenylacetic acid degradative pathway in Burkholderia cenocepacia K56-2. Using reporter activity assays, we show ... We previously made a link in Burkholderia cenocepacia between phenylacetic acid (PA) degradation and virulence by showing that ...
Characterization of the cciIR Quorum-Sensing System in Burkholderia cenocepacia | Infection and Immunity
Characterization of the cciIR Quorum-Sensing System in Burkholderia cenocepacia. Rebecca J. Malott, Adam Baldwin, Eshwar ... Characterization of the cciIR Quorum-Sensing System in Burkholderia cenocepacia. Rebecca J. Malott, Adam Baldwin, Eshwar ... Characterization of the cciIR Quorum-Sensing System in Burkholderia cenocepacia. Rebecca J. Malott, Adam Baldwin, Eshwar ... Several transmissible Burkholderia cenocepacia strains that infect multiple cystic fibrosis patients contain a genomic island ...
Microbiologics : 0836K Burkholderia cenocepacia derived from ATCC® 25608™* KWIK-STIK
The ATCC Licensed Derivative Emblem, the ATCC Licensed Derivative word mark and the ATCC catalog marks are trademarks of ATCC. Microbiologics, Inc. is licensed to use these trademarks and to sell products derived from ATCC® cultures. Look for the ATCC Licensed Derivative © Emblem for products derived from ATCC© cultures. ...
Identification of Essential Operons with a Rhamnose-Inducible Promoter in Burkholderia cenocepacia | Applied and Environmental...
Identification of Essential Operons with a Rhamnose-Inducible Promoter in Burkholderia cenocepacia. Silvia T. Cardona, Carmen L ... Identification of essential genes and essential operons in B. cenocepacia K56-2.Based on the previous results, B. cenocepacia ... Identification of Essential Operons with a Rhamnose-Inducible Promoter in Burkholderia cenocepacia ... Identification of Essential Operons with a Rhamnose-Inducible Promoter in Burkholderia cenocepacia ...
LPXC Recombinant Protein (Burkholderia cenocepacia) (OPCA05248) | E.coli Expressed Purified Protein
Burkholderia cenocepacia) (OPCA05248) , Alias: UDP-3-O-[R-3-hydroxymyristoyl]-N-acetylglucosamine deacetylase , MW: 51.00630092 ... Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia ( ... The genome of Burkholderia cenocepacia J2315, an epidemic pathogen of cystic fibrosis patients.. Holden M.T., Seth-Smith H.M., ...
σ54-Dependent Response to Nitrogen Limitation and Virulence in Burkholderia cenocepacia Strain H111 | Applied and Environmental...
σ54-Dependent Response to Nitrogen Limitation and Virulence in Burkholderia cenocepacia Strain H111. Martina Lardi, Claudio ... S1); consensus sequence logo for the B. cenocepacia H111 σ54-binding site (Fig. S2); growth delay of the B. cenocepacia H111 σ ... σ54-Dependent Response to Nitrogen Limitation and Virulence in Burkholderia cenocepacia Strain H111 ... σ54-Dependent Response to Nitrogen Limitation and Virulence in Burkholderia cenocepacia Strain H111 ...
Identification of Burkholderia cenocepacia Strain H111 Virulence Factors Using Nonmammalian Infection Hosts | Infection and...
A Burkholderia cenocepacia orphan LuxR homolog is involved in quorum-sensing regulation. J. Bacteriol. 191:2447-2460. ... Identification of Burkholderia cenocepacia Strain H111 Virulence Factors Using Nonmammalian Infection Hosts. Stephan Schwager, ... Burkholderia cenocepacia ZmpB is a broad-specificity zinc metalloprotease involved in virulence. Infect. Immun. 74:4083-4093. ... Burkholderia cenocepacia H111, a strain isolated from a cystic fibrosis patient, has been shown to effectively kill the ...
"The intracellular behaviour of Burkholderia cenocepacia in murine macrophages" by Jennifer S. Tolman
... cenocepacia-containing phagosome. We hypothesize that B. cenocepacia alters gene expression after internalization by ... In this work, we characterize later-stage infection of macrophages by B. cenocepacia, showing replication within an acidified ... As a whole, secretion systems appear to increase pathogenicity in macrophages while limiting the spread of B. cenocepacia ... Expression of secretion system genes is differentially-regulated by intracellular B. cenocepacia. Although none of the five ...
Quorum-sensing mutations affect attachment and stability of Burkholderia cenocepacia biofilms - Enlighten: Publications
Time-kill analysis of a stationary phase culture of Burkholderia cenocepacia strain LMG 16656 when exposed to geraniol (0.25%,...
Time-kill analysis of a stationary phase culture of Burkholderia cenocepacia strain LMG 16656 when exposed to geraniol (0.25%, ... Time-kill analysis of a stationary phase culture of Burkholderia cenocepacia strain LMG 16656 when exposed to geraniol (0.25%, ... Burkholderia cepacia gas chromatography analysis MBC GC ...
Differential Roles of RND Efflux Pumps in Antimicrobial Drug Resistance of Sessile and Planktonic Burkholderia cenocepacia...
Efflux pump genes of the resistance-nodulation-division family in Burkholderia cenocepacia genome. BMC Microbiol. 6:66. doi: ... Deciphering the role of RND efflux transporters in Burkholderia cenocepacia. PLoS One 6:e18902. doi:10.1371/journal.pone. ... Involvement of toxin-antitoxin modules in Burkholderia cenocepacia biofilm persistence. Pathog. Dis. 71:326-335. doi:10.1111/ ... Evaluation of the efficacy of disinfection procedures against Burkholderia cenocepacia biofilms. J. Hosp. Infect. 70:361-368. ...
J2315AbstractMultivoransInfectionInfectionsHI2424BiofilmsStrainsPathogensH111SpeciesIsolatesGeneGenesStrain LMG 16656Pathogen Burkholderia cenocepaciaPathogenicityMicrobiologyGenomovarBacteriaCharacterizationIsolateOrganismGenome Sequence of BurkholderiaType VI secretion systemPseudomonasMacrophagesAntimicrobialInducesBacterialComplexSequenceCystic fibrosis lungElegansAntibioticMutantsMutationLungIdentificationDraft genomeGenomic islandsLocusProteinVirulence factorsResistancePatients
J231524
- B. cenocepacia harbours a large, multi-replicon genome with a high GC-content, the reference genome of strain J2315 includes 7374 annotated genes. (nih.gov)
- RNA extracted from B. cenocepacia J2315 biofilms was analysed by differential RNA-sequencing and the resulting dataset compared to data derived from conventional, global RNA-sequencing. (nih.gov)
- Mapping transcription start sites across the B. cenocepacia genome added relevant information to the J2315 annotation. (nih.gov)
- In cystic fibrosis epidemic Burkholderia cenocepacia strain J2315, we identified a unique TAA (BCAM0224)-encoding gene, previously described as being implicated in virulence. (asm.org)
- In the present study, the effects of deletion of the 16 operons encoding resistance-nodulation-cell division (RND)-type efflux pumps in B. cenocepacia strain J2315 were investigated by determining the MICs of various antibiotics and by investigating the antibiofilm effect of these antibiotics. (asm.org)
- Our data indicate that the RND-3 and RND-4 efflux pumps are important for resistance to various antimicrobial drugs (including tobramycin and ciprofloxacin) in planktonic B. cenocepacia J2315 populations, while the RND-3, RND-8, and RND-9 efflux systems protect biofilm-grown cells against tobramycin. (asm.org)
- Results from the RT-qPCR experiments on the wild-type strain B. cenocepacia J2315 suggest that there is little regulation at the level of mRNA expression for these efflux pumps under the conditions tested. (asm.org)
- In the genome of B. cenocepacia strain J2315, a large number of efflux systems have been identified ( 7 - 10 ). (asm.org)
- The RND-3 (BCAL1674 to BCAL1676) and RND-4 (BCAL2820 to BCAL2822) efflux systems were shown to contribute to the intrinsic resistance of B. cenocepacia J2315 to various compounds and to mediate accumulation of quorum-sensing molecules in the growth medium ( 8 ). (asm.org)
- Genome analysis and homology searches revealed 14 open reading frames encoding putative drug efflux pumps belonging to RND family in B. cenocepacia J2315 strain. (biomedcentral.com)
- High levels of AGS3 in macrophages enhanced their resistance to infection by Burkholderia cenocepacia J2315, Mycobacterium tuberculosis , and methicillin-resistant Staphylococcus aureus , whereas AGS3-deficient macrophages were more susceptible. (jimmunol.org)
- Vacuoles containing B. cenocepacia strain J2315, an isolate of the transmissible ET12 clone, recruited Rab5 and synthesized phosphatidylinositol-3-phosphate, indicating progression to the early phagosomal stage. (qub.ac.uk)
- Fang2011 - Genome-scale metabolic network of Burkholderia cenocepacia (iKF1028) This model is described in the article: Exploring the metabolic network of the epidemic pathogen Burkholderia cenocepacia J2315 via genome-scale reconstruction. (omicsdi.org)
- Strain B. cenocepacia J2315 is the most infectious isolate from CF patients. (omicsdi.org)
- There is a strong demand to reconstruct a genome-scale metabolic network of B. cenocepacia J2315 to systematically analyze its metabolic capabilities and its virulence traits, and to search for potential clinical therapy targets. (omicsdi.org)
- RESULTS: We reconstructed the genome-scale metabolic network of B. cenocepacia J2315. (omicsdi.org)
- The model iKF1028 captures important metabolic capabilities of B. cenocepacia J2315 with a particular focus on the biosyntheses of key metabolic virulence factors to assist in understanding the mechanism of disease infection and identifying potential drug targets. (omicsdi.org)
- Based on the model, the genome annotation of B. cenocepacia J2315 was refined and 24 genes were properly re-annotated. (omicsdi.org)
- CONCLUSIONS: As the first genome-scale metabolic network of B. cenocepacia J2315, iKF1028 allows a systematic study of the metabolic properties of B. cenocepacia and its key metabolic virulence factors affecting the CF community. (omicsdi.org)
- 1] Transcription profiling of one Burkholderia cenocepacia clinical isolate, J2315, versus a soil isolate, HI2424, in conditions mimicking CF sputum [2] Transcription profiling of Burkholderia cenocepacia isolates J2315 and HI2424 in media mimicking CF sputum or the soil environment Overall design: [1] J2315 vs. HI2424 cells in the same condition. (omicsdi.org)
- Burkholderia cenocepacia J2315 belongs to the ET12 lineage which has caused outbreaks among cystic fibrosis patients worldwide. (omicsdi.org)
- DNA probes for IS commonly found in B. cenocepacia were designed using the B. cenocepacia J2315 genome. (plymouth.ac.uk)
- Interestingly, when the closely related B. cenocepacia strain J2315 was exposed to oxidative stress, no movement of ISBcen20 was detected. (plymouth.ac.uk)
- Burkholderia cenocepacia J2315 escapes to the cytosol and actively subverts autophagy in human macrophages. (microbiologyresearch.org)
Abstract1
- 5: 83 Abstract: BACKGROUND: Burkholderia cenocepacia is a threatening nosocomial epidemic pathogen in patients with cystic fibrosis (CF) or a compromised immune system. (omicsdi.org)
Multivorans13
- All genomovars have been shown to cause infections, and B. cenocepacia and B. multivorans (genomovars III and II, respectively) are the genomovars most frequently isolated from CF patients ( 4 - 7 ). (cdc.gov)
- The IST439 OAg biosynthetic gene cluster contains 7 of 23 genes that are closely homologous to genes found in B. multivorans , another member of the Burkholderia cepacia complex. (frontiersin.org)
- B. cenocepacia and Burkholderia multivorans are currently the Bcc species most frequently isolated from clinical samples ( 1 , 4 ). (asm.org)
- Although there are considerable regional differences, the majority of patients with cystic fibrosis worldwide are infected with either Burkholderia multivorans or Burkholderia cenocepacia ( 2 ). (asm.org)
- B. cenocepacia , B. multivorans , B. stabilis , B. contaminans , and B. vietnamiensis [ 1 ]. (hindawi.com)
- B. cenocepacia and Burkholderia multivorans account for the majority of infections in CF, and major epidemic clones have been recognized throughout the world. (cdc.gov)
- Burkholderia cepacia genomovars I and VI, Burkholderia multivorans (genomovar II), Burkholderia cenocepacia (genomovar III), Burkholderia stabilis (genomovar IV), Burkholderia vietnamiensis (genomovar V), Burkholderia ambifaria (genomovar VII), Burkholderia anthina (genomovar VIII), and Burkholderia pyrrocinia (genomovar IX) ( 2 , 9 , 10 ). (asm.org)
- B. cenocepacia (n=6), B. multivorans (n=3), B. stabilis (n=3). (ersjournals.com)
- After 5 yrs, three B. cenocepacia - and one B. multivorans -colonised patients had died. (ersjournals.com)
- B. multivorans (n=9), B. stabilis (n=1) and B. cenocepacia (n=2). (ersjournals.com)
- Burkholderia multivorans in 1999. (ersjournals.com)
- The most commonly isolated Bcc species in CF patients are B. multivorans, B. cenocepacia and B. stabilis . (ersjournals.com)
- B. multivorans , B. cenocepacia and B. stabilis ) 10 . (ersjournals.com)
Infection29
- Burkholderia cenocepacia infection often leads to fatal cepacia syndrome in cystic fibrosis patients. (nih.gov)
- We report a case of a vaginal infection caused by a strain of Burkholderia cenocepacia . (cdc.gov)
- We report a case of vaginal infection, caused by B. cenocepacia, in a patient affected by smoldering myeloma, and chronic hepatitis C virus (HCV) infection. (cdc.gov)
- Burkholderia cenocepacia is an opportunistic pathogen associated with chronic lung infections and increased risk of death in patients with cystic fibrosis (CF). In this work, we investigated the lipopolysaccharide (LPS) of clinical variants of B. cenocepacia that were collected from a CF patient over a period of 3.5 years, from the onset of infection until death by necrotizing pneumonia (cepacia syndrome). (frontiersin.org)
- This study provides support to the notion that OAg LPS modifications are an important factor in the adaptation of B. cenocepacia to chronic infection and that the heterogeneity of OAgs relates to variation within the OAg gene cluster, indicating that the gene cluster might have been assembled through multiple horizontal transmission events. (frontiersin.org)
- We previously made a link in Burkholderia cenocepacia between PA degradation and virulence by showing that insertional mutagenesis of paaA and paaE genes, that encode part of a multicomponent oxidase of PA-CoA, results in PA-conditional growth and an attenuated killing phenotype in the Caenorhabditis elegans model of infection. (frontiersin.org)
- We used the C. elegans model of infection to screen a mini-Tn 5 mutant library of B. cenocepacia H111 for attenuated virulence. (asm.org)
- In this work, we characterize later-stage infection of macrophages by B. cenocepacia , showing replication within an acidified endosomal compartment suggestive of a phagolysosome. (uwo.ca)
- As a whole, secretion systems appear to increase pathogenicity in macrophages while limiting the spread of B. cenocepacia infection. (uwo.ca)
- Loutet, SA & Valvano, M 2010, ' A Decade of Burkholderia cenocepacia Virulence Determinant Research ', Infection and Immunity , vol. 78, no. 10, pp. 4088-4100. (qub.ac.uk)
- Members of the Burkholderia cepacia complex, normally present in soil microbiota, cause nosocomial lung infection especially in hospitalized cystic fibrosis patients. (microbiologyresearch.org)
- Transcriptomic profiling across nine growth conditions was performed to identify the global gene expression changes made when B. cenocepacia changes niches from an environmental lifestyle to infection. (cf.ac.uk)
- By creating isogenic PSCP mutants, we determined that PSCP is required for the maximal virulence of B. cenocepacia K56-2 in an experimental model of chronic lung infection. (ucalgary.ca)
- IST439 was the first B. cenocepacia isolate recovered from the patient and it is thought to have initiated the infection while the IST4113 isolate was recovered three years later, after a period of exacerbated infection that compelled the patient to hospitalization and intravenous therapy with gentamicine and ceftazidime. (omicsdi.org)
- SARS-CoV-2 and Burkholderia cenocepacia infection in a patient with Cystic Fibrosis: An unfavourable conjunction? (cdc.gov)
- The effects of the concomitant infection by COVID-19 and Burkholderia cepacia (Bc) in CF are not known. (cdc.gov)
- Clinafloxacin for Treatment of Burkholderia cenocepacia Infection in a" by Akshu Balwan, David P Nicolau et al. (mainehealth.org)
- Clinafloxacin for Treatment of Burkholderia cenocepacia Infection in a Cystic Fibrosis Patient. (mainehealth.org)
- Respiratory infection with Burkholderia cenocepacia is associated with accelerated decline in lung function and increased mortality in cystic fibrosis (CF) patients (A. M. Jones, M. E. Dodd, J. R. W. Govan, V. Barcus, C. J. Doherty, J. Morris, and A. K. Webb, Thorax 59:948-951, 2004, http://dx.doi.org/10.1136/thx.2003.017210). (mainehealth.org)
- B. cenocepacia often possesses innate resistance to multiple antimicrobial classes, making eradication uncommon in established infection (P. B. Davis, Am J Respir Crit Care Med 173:475-482, 2006, http://dx.doi.org/10.1164/rccm.200505-840OE). (mainehealth.org)
- We report the use of clinafloxacin in a CF patient with advanced B. cenocepacia infection, present pharmacokinetic (PK) data, and discuss the potential therapeutic role of clinafloxacin in patients with this condition. (mainehealth.org)
- Here we constructed and characterized a B. cenocepacia tonB mutant strain, which was unable to actively transport iron, to test whether this single gene deletion mutant (strain renamed GAP001) protected against an acute respiratory B. cenocepacia lethal infection. (utmb.edu)
- Chronic infection with opportunistic pathogens including Burkholderia cepacia complex (Bcc) is a hallmark of cystic fibrosis (CF). We investigated the adaptive mechanisms facilitating chronic lung infection in sequential Bcc isolates from two siblings with CF (P1 and P2), one of whom also experienced intermittent blood-stream infections (P2). (maynoothuniversity.ie)
- Agodi A , Mahenthiralingam E , Bachitta M , Giannino V , Sciacca A , Stefani S . Burkholderia cepacia complex infection in Italian patients with cystic fibrosis: prevalence, epidemiology, and genomovar status. (cdc.gov)
- Burkholderia cenocepacia infection: disruption of phagocyte immune functions through Rho GTPase inactivation. (qxmd.com)
- Patients positive for human immunodeficiency virus (HIV) or Burkholderia cenocepacia infection. (clinicaltrials.gov)
- Burkholderia cenocepacia has been a very hard to treat bacterial infection for our Cystic Fibrosis community. (cysticfibrosis.com)
- Burkholderia cepacia infection is much less common, but it is notorious for being associated with cross infection and the possibility of rapid deterioration, known as the cepacia syndrome 2 - 4 . (ersjournals.com)
- A rapid deterioration and patient-to-patient transmission is probably more likely with B. cenocepacia infection and colonisation 8 , 9 . (ersjournals.com)
Infections25
- Infections with B. cenocepacia are very difficult to treat due to their high intrinsic resistance to most antibiotics. (nih.gov)
- In particular, the lack of efficacious antimicrobial treatments for infections caused by the Burkholderia cepaci a complex is causing high morbidity and mortality of cystic fibrosis patients and of patients with nosocomial infections. (asm.org)
- Burkholderia cenocepacia is an opportunistic pathogen causing life-threatening infections in cystic fibrosis and other immunocompromised patients. (uwo.ca)
- Burkholderia cenocepacia is notorious for causing respiratory tract infections in people with cystic fibrosis. (asm.org)
- Species belonging to the Burkholderia cepacia complex (Bcc), a cluster of phylogenetically closely related and phenotypically similar Gram-negative bacteria, can cause severe respiratory tract infections in people with cystic fibrosis ( 1 ). (asm.org)
- Infections with B. cenocepacia are particularly difficult to treat due to their high level of resistance against a wide range of antimicrobial agents ( 1 , 3 , 4 ). (asm.org)
- The Burkholderia cepacia complex (Bcc) is a group of genetically related environmental bacteria that can cause chronic opportunistic infections in patients with cystic fibrosis (CF) and other underlying diseases. (qub.ac.uk)
- A common theme emerging from these studies is that B. cenocepacia establishes chronic infections in immuno-compromised patients, which depend more on determinants mediating host niche adaptation than those involved directly in host cells and tissue damage. (qub.ac.uk)
- Burkholderia cenocepacia is recognized as opportunistic pathogen that can cause lung infections in cystic fibrosis patients. (biomedcentral.com)
- A hallmark of B. cenocepacia infections is the inability to eradicate the organism because of multiple intrinsic antibiotic resistance. (biomedcentral.com)
- B. cenocepacia is known for its ability to cause lung infections in people with cystic fibrosis and it possesses a large 8 Mb multireplicon genome encoding a wide array of pathogenicity and fitness genes. (cf.ac.uk)
- These infections are caused, among others, by opportunistic pathogens, for example Burkholderia cenocepacia(B. cenocepacia). (knowledgeforgrowth.be)
- Pseudomonas aeruginosa and Burkholderia cenocepacia are the causative agents of chronic airway infections and a major cause of mortality in cystic fibrosis patients. (ucalgary.ca)
- Despite advances in anti-microbial therapy, the treatment of infections caused by P. aeruginosa and B. cenocepacia remains a challenge. (ucalgary.ca)
- These studies provide the initial steps toward producing anti-peptide 15 immunotherapeutic agents for use in P. aeruginosa or B. cenocepacia respiratory infections. (ucalgary.ca)
- A glycoengineered antigen exploiting a conserved protein O-glycosylation pathway in the Burkholderia genus for detection of glanders infections. (pubfacts.com)
- Burkholderia cenocepacia can cause serious infections and epidemics in patients with cystic fibrosis (CF). A CF population in the Czech Republic experienced an epidemic outbreak caused by a B. cenocepacia ST-32 strain. (plymouth.ac.uk)
- Burkholderia cenocepacia is an opportunistic pathogen prevalent in cystic fibrosis patients, which is particularly difficult to treat, causing chronic and eventually fatal infections. (utmb.edu)
- Vaccines, and live attenuated vaccines, are an unexplored avenue to treat B. cenocepacia infections. (utmb.edu)
- The Burkholderia cepacia complex ( Bcc ) bacteria are opportunistic pathogens which cause nosocomial infections and are especially dangerous for cystic fibrosis (CF) patients. (hindawi.com)
- In the early 1980s Burkholderia cepaciabecame associated with epidemics of severe lung infections ('cepacia syndrome') traced, in many cases, to CF treatment centers and social gatherings where CF patients apparently acquired the disease from other B. cepacia-infected individuals (19). (apsnet.org)
- In this environment, we studied adaptation by a soil isolate of Burkholderia cenocepacia , an opportunistic pathogen responsible for fatal biofilm-associated lung infections in CF patients ( 14 , 15 ). (pnas.org)
- Pseudomonas and Burkholderia bacteria cause devastating infections in people with cystic fibrosis and we have used molecular biology and genomic approaches to track their ability to spread between patients, resist killing by antibiotics and cause lung disease. (cardiff.ac.uk)
- For example, our recent discovery that Burkholderia bacteria can produce novel polyketide antibiotics that target multidrug resistant bacterial infections is very exciting. (cardiff.ac.uk)
- This is where I began to developing expertise in the cystic fibrosis microbiology, working on Pseudomonas aeruginosa and Burkholderia cepacia complex bacteria that cause devastating lung infections in these individuals. (cardiff.ac.uk)
HI24241
- CC Burkholderia cenocepacia HI2424 plasmid 1, complete sequence. (univ-lyon1.fr)
Biofilms2
- Four thousand ten transcription start sites were mapped over the whole B. cenocepacia genome and the primary transcription start site of 2089 genes expressed in B. cenocepacia biofilms were defined. (nih.gov)
- Contributing to this is the fact that Bcc strains, including B. cenocepacia strains, readily form biofilms on various biotic and abiotic surfaces ( 5 ). (asm.org)
Strains12
- The consensus phylogenetic tree of recA DNA sequences of Burkholderia cepacia complex strains, representative of each genomovar, and of the B. cenocepacia isolate (GenBank accession no. (cdc.gov)
- Several transmissible Burkholderia cenocepacia strains that infect multiple cystic fibrosis patients contain a genomic island designated as the c eno c epacia i sland (cci). (asm.org)
- B. cenocepacia strains also have a cepIR quorum-sensing system. (asm.org)
- The majority of transmissible or epidemic Bcc strains are B. cenocepacia ( 26 , 31 , 48 , 59 ), and epidemiological studies have shown this species is highly virulent, causing significant mortality among CF patients ( 28 , 30 ). (asm.org)
- The two quorum sensing systems exert reciprocal regulation of many genes likely enabling fine-tuned control of quorum sensing gene expression in B. cenocepacia strains carrying the cenocepacia island. (biomedcentral.com)
- Distribution of cepacian biosynthesis genes among environmental and clinical Burkholderia strains and role of cepacian exopolysaccharide in resistance to stress conditions. (burkholderia.com)
- Strains of the Burkholderia cepacia complex can survive within macrophages by arresting the maturation of phagocytic vacuoles. (qub.ac.uk)
- Strains belonging to the ET12 lineage are characterized by the presence of the "Burkholderia cepacia epidemic strain marker" (BCESM). (omicsdi.org)
- Individual normalized intensity of Burkholderia cenocepacia and Burkholderia seminalis hybridized in a B. cenocepacia array Overall design: A collection of 199 Burkholderia isolates were hybridized against an array targeting five B. cenocepacia strains in order to understand the gene distribution within this species. (omicsdi.org)
- Different species Burkholderia and Achromobacter strains all demonstrated similar BiofilmReg operon structure. (hindawi.com)
- Only recA DNA sequences of reference B. cenocepacia strains (genomovar III, lineage IIIB) are included in the tree ( 4 , 13 ). (cdc.gov)
- We report here that deletion of BCAM0628, encoding a predicted low molecular weight protein tyrosine phosphatase (LMW-PTP) that is restricted to B. cenocepacia strains of the transmissible ET-12 clone, accelerates the maturation of the BcCVs. (microbiologyresearch.org)
Pathogens10
- Over the past 20 years a group of closely related Burkholderia species termed the B. cepacia complex (Bcc) have emerged as opportunistic pathogens of particular importance in people with cystic fibrosis (CF) and chronic granulomatous disease ( 31 , 33 ). (asm.org)
- The Burkholderia cepacia complex is a group of gram-negative bacteria comprising at least nine species ( 33 , 48 ) which have emerged as multidrug-resistant nosocomial pathogens in immunocompromised patients, particularly in those with chronic granulomatous diseases and cystic fibrosis. (asm.org)
- Burkholderia cenocepacia belongs to a group of closely related organisms called the B. cepacia complex (Bcc) which are important opportunistic human pathogens. (biomedcentral.com)
- Burkholderia cenocepacia is a Gram-negative aerobic bacterium that belongs to a group of opportunistic pathogens displaying diverse environmental and pathogenic lifestyles. (cf.ac.uk)
- Here, we will discuss six zebrafish models for important human pathogens ( Mycobacterium , Salmonella , Burkholderia , Staphylococcus , Shigella and Candida ), emphasizing the novel insights that these models have recently provided into macrophage biology and highlighting how this could lead to the finding of new host-derived therapeutic strategies. (biologists.org)
- As B. cenocepacia and C. albicans are frequently encountered human pathogens, identification of the BDSF signal and its activity thus provides a new insight into the molecular grounds of their antagonistic interactions whose importance to microbial ecology and pathogenesis is now becoming evident. (pubfacts.com)
- Organisms of the Burkholderia cepacia complex are especially important pathogens in cystic fibrosis (CF), with a propensity for patient-to-patient spread and long-term respiratory colonization. (cdc.gov)
- The Burkholderia cepacia complex (Bcc) is a group of at least 9 closely related species (often called "genomovars") that is currently attracting considerable attention for their extraordinary versatility as plant pathogens, saprophytes, biocontrol agents, bioremediation agents, and human pathogens. (apsnet.org)
- My group has studied the pathogenesis and ecology of bacterial opportunistic pathogens, ranging from Pseudomonas aeruginosa to mycobacteria, with a major focus on Burkholderia cepacia complex bacteria. (cardiff.ac.uk)
- In the last few years, we made a very exciting discovery that the Burkholderia bacteria I had studied as pathogens, also make some very potent antibiotics which kill other multidrug resistant bacteria and fungi. (cardiff.ac.uk)
H11110
- A = averages) shows the log 2 fold changes in transcript expression in B. cenocepacia H111 grown under nitrogen-limited (S) versus nitrogen-replete (N) conditions. (asm.org)
- Box plot for the numbers of reads per kilobase per million (RPKM) for genes in the wild-type strain B. cenocepacia H111, the σ 54 mutant (mt), and the complemented mutant (comp). (asm.org)
- Cells of the wild-type strain B. cenocepacia H111 (B and F), the σ 54 mutant (C and G), and the complemented mutant (D and H) were grown on plates containing limiting amounts of ammonium. (asm.org)
- C) Swimming motility of the wild-type strain B. cenocepacia H111, the σ 54 mutant, and the complemented mutant. (asm.org)
- Virulence of B. cenocepacia H111 against C. elegans is dependent on σ 54 . (asm.org)
- Using a discovery shotgun proteomics approach, the expressed proteome of Burkholderia cenocepacia (strain H111) was analyzed under normal and starved nitrogen growth conditions. (omicsdi.org)
- The opportunistic human pathogen Burkholderia cenocepacia H111 uses two chemically distinct signal molecules for controlling gene expression in a cell density-dependent manner: N-acyl-homoserine lactones (AHLs) and cis-2-dodecenoic acid (BDSF). (unibas.ch)
- In this study we analysed the transcriptional profile of B. cenocepacia H111 upon artificially altering the cellular c-di-GMP level. (unibas.ch)
- In dieser Arbeit wurde der Nematode Caenorhabditis elegans als Modell zur Untersuchung der Pathogenität des klinischen Isolats B. cenocepacia H111 verwendet. (tum.de)
- Durch den Einsatz spezifischer QS-Hemmstoffe konnte die Pathogenität von B. cenocepacia H111 und dem bei Patienten mit Mukoviszidose vorherrschenden Stamm, Pseudomonas aeruginosa, erheblich vermindert werden. (tum.de)
Species20
- Burkholderia cenocepacia is a species of Gram-negative bacteria that is common in the environment, can form a biofilm with itself, is resistant to many antibiotics and may cause disease in plants. (wikipedia.org)
- See also: Burkholderia thailandensis sRNA Originally defined as B. cepacia, the group has now been split into nine species, and B. cenocepacia is one of the most intensively-studied. (wikipedia.org)
- To date, the genus Burkholderia comprises more than 30 species, including the Burkholderia cepacia complex, B. mallei , and B. pseudomallei ( 2 ). (cdc.gov)
- Whole-Genome Assemblies of 56 Burkholderia Species. (kegg.jp)
- B. cenocepacia is the most commonly reported Bcc species isolated from patients with CF ( 26 , 48 ). (asm.org)
- The Burkholderia cepacia complex (Bcc) consists of 17 closely related, but phenotypically diverse, bacterial species. (asm.org)
- Burkholderia cenocepacia has been the focus of attention because initially it was the most common Bcc species isolated from patients with CF in North America and Europe. (qub.ac.uk)
- Transcriptional profiling of RNA-seq data from two Burkholderia species grown under conditions mimicking the cystic fibrosis lung and the soil environment Overall design: Two condition, two strain experiment. (omicsdi.org)
- Pseudomonas aeruginosa and species of the Burkholderia cepacia complex (Bcc) can co-exist in the lower airways, however several studies have shown that P. aeruginosa can effectively kill the Bcc in vitro, for which hydrogen cyanide (HCN) was recently proposed to play a critical role. (dartmouth.edu)
- Here we show that modification of the environment (i.e., culture medium), long-term genetic adaptation of P aeruginosa to the cystic fibrosis (CF) lung, or the addition of another bacterial species to the community can alter the sensitivity of Burkholderia cenocepacia to P aeruginosa toxins. (dartmouth.edu)
- Among these, B. cenocepacia was more abundant, and the Russian epidemic strain ST (sequence type) 709 belonged to this species. (hindawi.com)
- Coenye T , Vandamme P . Diversity and significance of Burkholderia species occupying diverse ecological niches. (cdc.gov)
- Molecular epidemiology of Burkholderia species. (cdc.gov)
- Spilker T, Gill GH, Campbell PW, Liu L, Mahenthiralingam E. Disproportionate distribuition of Burkholderia cepacia complex species and transmissibility markers in cystic fibrosis. (cdc.gov)
- Vermis K , Coenye T , Mahenthiralingam E , Nelis HJ , Vandamme P . Evaluation of species-specific recA -based PCR tests for genomovar level identification within the Burkholderia cepacia complex. (cdc.gov)
- Compared to the parental strain and deletion mutants in other LMW-PTPs that are widely conserved in Burkholderia species, a greater proportion of BcCVs containing the Δ BCAM0628 mutant were targeted to the lysosome. (microbiologyresearch.org)
- Burkholderia cepacia is the type species of the genus Burkholderia and is a ubiquitous multidrug-resistant, motile, non-glucose-fermenting, gram-negative organism found in water and soil ( 1 ). (cdc.gov)
- Typically, each species of Burkholderia has 3-5 homologous tsRNAs. (wikipedia.org)
- Experiments with four species of the Burkholderia lineage showed conserved and constitutive expression of tsRNAs in logarithmic growth phases. (wikipedia.org)
- Although this species has been identified as the most prevalent and pathogenic of the Burkholderia cepacia complex species in CF patients ( 14 ), relatively little is known about how it adapts to a biofilm lifestyle or to the CF lung environment ( 5 , 16 ). (pnas.org)
Isolates3
- DNA-DNA hybridisation experiments between isolates representing Burkholderia cepacia genomovar III recA lineages IIIA and IIIB reinforced the classification of both phylogenetic subgroups as a single genospecies, distinct from B. cepacia (genomovar I). A formal classification of B. cepacia genomovar III encompassing the recA lineages IIIA and IIIB, and the new recA lineages IIIC and IIID, as B. cenocepacia sp. (nih.gov)
- Petrucca A , Cipriani P , Valenti P , Santapaola D , Cimmino C , Scoarughi GL , Molecular characterization of Burkholderia cepacia isolates from cystic fibrosis (CF) patients in an Italian CF center. (cdc.gov)
- These mutations in turn were surprisingly well represented among mutations that evolved in cystic fibrosis isolates of both Burkholderia and Pseudomonas . (pnas.org)
Gene13
- AY278465 Burkholderia cenocepacia strain LMG 16656 outer membrane lipoprotein precursor (opcL) gene, partial cds. (atcc.org)
- AF148556 Burkholderia cepacia genomovar III 16S ribosomal RNA gene, partial sequence. (atcc.org)
- An antibiotic efflux gene cluster that confers resistance to chloramphenicol, trimethoprim, and ciprofloxacin has been identified in Burkholderia cenocepacia (genomovar III), an important cystic fibrosis pathogen. (jci.org)
- Using reporter activity assays, we demonstrate that 3-OHPA activated PA degradation gene promoters of Burkholderia cenocepacia K56-2 in a paaK1 -dependent manner, while 4-OHPA had no effect. (frontiersin.org)
- We hypothesize that B. cenocepacia alters gene expression after internalization by macrophages, inducing genes involved in intracellular survival and host adaptation. (uwo.ca)
- We examine differential gene expression by intracellular B. cenocepacia using selective capture of transcribed sequences (SCOTS) with both competitive enrichment and microarray analysis. (uwo.ca)
- B. cenocepacia utilizes a mechanism of cell-cell communication called quorum sensing to control gene expression including genes involved in virulence. (biomedcentral.com)
- Global gene expression profiles during growth in stationary phase were generated using microarrays of B. cenocepacia cepR , cciR and cepRcciIR mutants. (biomedcentral.com)
- For the first time, the influence of CciIR on global gene regulation in B. cenocepacia has been elucidated. (biomedcentral.com)
- The 14 RND pumps gene we have identified in the genome of B. cenocepacia suggest that active efflux could be a major mechanism underlying antimicrobial resistance in this microorganism. (biomedcentral.com)
- In this study, we cloned and sequenced the zmpA gene encoding for PSCP, an extracellular metalloprotease of B. cenocepacia. (ucalgary.ca)
- The gene is highly conserved and widespread in the Burkholderia cepacia complex. (pubfacts.com)
- Dabei wurde die Bedeutung der QS-Gene cepR und rsuA für die Pathogenität von B. cenocepacia bestätigt. (tum.de)
Genes13
- Genes and novel regulatory RNAs putatively involved in B. cenocepacia biofilm formation were identified. (nih.gov)
- In this study, we utilized TraDIS (Transposon Directed Insertion-site Sequencing) as a genome-wide screening tool to facilitate the identification of B. cenocepacia genes essential for its growth and viability. (nih.gov)
- The saturated library allowed for the identification of 383 genes that were predicted to be essential in B. cenocepacia. (nih.gov)
- We extended the application of TraDIS to identify conditionally essential genes required for in vitro growth and revealed an additional repertoire of 439 genes to be crucial for B. cenocepacia growth under nutrient-depleted conditions. (nih.gov)
- The library of B. cenocepacia mutants can subsequently be subjected to various biologically related conditions to facilitate the discovery of genes involved in niche adaptation as well as pathogenicity and virulence. (nih.gov)
- Here, we present a method based on delivery of the tightly regulated rhamnose-inducible promoter P rhaB for identifying essential genes and operons in Burkholderia cenocepacia . (asm.org)
- This system allows us to identify essential genes and operons in B. cenocepacia and provides a new tool for systematically identifying and functionally characterizing essential genes at the genomic level. (asm.org)
- Deletion of various SCOTS-identified genes affects B. cenocepacia entry into macrophages and intracellular replication, as well as host-directed cytotoxicity and spread to neighbouring cells. (uwo.ca)
- Expression of secretion system genes is differentially-regulated by intracellular B. cenocepacia . (uwo.ca)
- Further investigation into the remaining SCOTS-identified genes, as well as putative secreted effectors, will provide a better understanding of the adaptive responses of intracellular B. cenocepacia , leading to life in a phagosomal niche and host cell cytotoxicity. (uwo.ca)
- Novel genes under the control of the CepIR and CciIR quorum sensing systems in B. cenocepacia have been identified. (biomedcentral.com)
- As Resistance-Nodulation-Division (RND) efflux systems are responsible for much of the intrinsic multidrug resistance in Gram-negative bacteria, this study aims to identify RND genes in the B. cenocepacia genome and start to investigate their involvement into antimicrobial resistance. (biomedcentral.com)
- Previous work demonstrated that B. cenocepacia phenylacetic acid (PA) catabolic genes are up-regulated during growth in SCFM and are required for full virulence in a Caenorhabditis elegans host model. (umanitoba.ca)
Strain LMG 166561
- Time-kill analysis of a stationary phase culture of Burkholderia cenocepacia strain LMG 16656 when exposed to geraniol (0.25%, v/v) and 5 mM EDTA. (figshare.com)
Pathogen Burkholderia cenocepacia1
- Delayed association of the NADPH oxidase complex with macrophage vacuoles containing the opportunistic pathogen Burkholderia cenocepacia. (qxmd.com)
Pathogenicity1
- Here, we characterized this multifunctional protein, trying to establish its role in B. cenocepacia pathogenicity. (asm.org)
Microbiology2
- The book was edited by Tom Coenye and myself and serves as an excellent update to the highly successful first book: "Burkholderia: molecular microbiology and genomics" ( http://www.horizonpress.com/backlist/horizonbioscience/burk.html ). (cardiff.ac.uk)
- While Burkholderia bacteria and cystic fibrosis microbiology remain major research foci, my interests are still wide and I am always getting involved in lots of very interesting molecular microbiology projects. (cardiff.ac.uk)
Genomovar1
- Phenotypic methods for determining genomovar status of the Burkholderia cepacia complex. (cdc.gov)
Bacteria4
- Burkholderia cenocepacia is a member of the B. cepacia complex (Bcc), a group of opportunistic bacteria that infect the airways of patients with cystic fibrosis (CF) and are extraordinarily resistant to almost all clinically useful antibiotics. (microbiologyresearch.org)
- nov. and Burkholderia pyrrocinia , two additional Burkholderia cepacia complex bacteria, may confound results of new molecular diagnostic tools. (cdc.gov)
- Additionally, they are not toxic to the bacteria in which these RNA molecules are found (B. cenocepacia). (wikipedia.org)
- We are pleased to announced the forthcoming publication of a second book on Burkholderia bacteria: "Burkholderia: from genomes to function" which can be previewed at Horizon Press ( http://www.horizonpress.com/burk2 ). (cardiff.ac.uk)
Characterization4
- The characterization of antibiotic efflux pumps in B. cenocepacia is an obligatory step prior to the design of specific, potent bacterial inhibitors for the improved control of infectious diseases. (biomedcentral.com)
- We report here the isolation and characterization of a novel signaling molecule, cis-2-dodecenoic acid (BDSF), from Burkholderia cenocepacia. (pubfacts.com)
- Characterization of the secondary structures of 4 tsRNAs in Burkholderia cenocepacia suggests they form double hairpin structures. (wikipedia.org)
- Characterization of rhamnolipid production by Burkholderia glumae. (springer.com)
Isolate2
- The OAg repeating unit of the LPS from IST439, the initial isolate, is a [→2)-β-D-Rib f -(1→4)-α-D-Gal p NAc-(1→] disaccharide, which was not previously described in B. cenocepacia . (frontiersin.org)
- Burkholderia cenocepacia is an environmental organism and an unusual isolate from blood, although is a recognised cause of nosocomial outbreaks due to contamination of various fluids. (acipcconference.com.au)
Organism2
- In addition, the strong environmental protection response of B. cenocepacia is attributed to the biofilm formed by groups of the organism. (wikipedia.org)
- Whether Burkholderia cepacia complex should be an objectionable organism in antiseptic solutions with acceptable total bacterial counts is controversial. (cdc.gov)
Genome Sequence of Burkholderia2
- Complete Genome Sequence of Burkholderia cenocepacia Phage Magia. (pubfacts.com)
- Complete Genome Sequence of Burkholderia cenocepacia Phage Mica. (pubfacts.com)
Type VI secretion system4
- A novel sensor kinase-response regulator hybrid controls biofilm formation and type VI secretion system activity in Burkholderia cenocepacia. (burkholderia.com)
- Here, we show that intracellular B. cenocepacia expressing a type VI secretion system (T6SS) affects the activation of the Rac1 and Cdc42 RhoGTPase by reducing the cellular pool of GTP-bound Rac1 and Cdc42. (qxmd.com)
- Burkholderia cenocepacia type VI secretion system mediates escape of type II secreted proteins into the cytoplasm of infected macrophages. (qxmd.com)
- Quantification of type VI secretion system activity in macrophages infected with Burkholderia cenocepacia. (qxmd.com)
Pseudomonas2
- Extracellular metalloproteases of pseudomonas aeruginosa and burkholderia cenocepacia as potential immunotherapeutic targets (Unpublished doctoral thesis). (ucalgary.ca)
- Formerly known as Pseudomonas cepacia, the bacterium now known as Burkholderia cepacia, was first described in 1950 as the cause of sour skin of onions by Cornell University plant pathologist Walter Burkholder (1). (apsnet.org)
Macrophages6
- The bacterium survives within macrophages by interfering with typical endocytic trafficking, resulting in delayed maturation of a B. cenocepacia -containing phagosome. (uwo.ca)
- Together, these studies advance our understanding of the intracellular behaviour of B. cenocepacia in macrophages. (uwo.ca)
- B. cenocepacia survives inside macrophages, and this survival depends on the secretion of virulence factors, among which two (metallo)proteases. (knowledgeforgrowth.be)
- We have previously shown that B. cenocepacia can survive in macrophages within membrane vacuoles [ B. cenocepacia -containing vacuoles (BcCVs)] that preclude fusion with the lysosome. (microbiologyresearch.org)
- Depletion of the ubiquitin-binding adaptor molecule SQSTM1/p62 from macrophages harboring cftr ΔF508 mutation improves the delivery of Burkholderia cenocepacia to the autophagic machinery. (microbiologyresearch.org)
- Therefore, T6SS-mediated deregulation of Rho family GTPases is a common mechanism linking disruption of the actin cytoskeleton and delayed NADPH oxidase activation in macrophages infected with B. cenocepacia. (qxmd.com)
Antimicrobial2
- Its overexpression in E. coli conferred resistance to several antibiotics and to ethidium bromide but it remains to be determined if this pump play a significant role in the antimicrobial intrinsic resistance of B. cenocepacia . (biomedcentral.com)
- Nzula S , Vandamme P , Govan JR . Influence of taxonomic status on the in vitro antimicrobial susceptibility of the Burkholderia cepacia complex. (cdc.gov)
Induces2
- Oxidative stress of Burkholderia cenocepacia induces insertion sequence-mediated genomic rearrangements that interfere with macrorestriction-based genotyping. (plymouth.ac.uk)
- Burkholderia cenocepacia induces neutrophil necrosis in chronic granulomatous disease. (medscape.com)
Bacterial3
- Here, we used RNA-seq to comprehensively and quantitatively assess the transcriptional response of the bacterial opportunistic cystic fibrosis (CF) pathogen and endemic soil dweller, Burkholderia cenocepacia, in conditions mimicking these 2 environments. (omicsdi.org)
- Further analyses showed that Candida albicans germ tube formation was strongly inhibited by either coculture with B. cenocepacia or by exogenous addition of physiological relevant levels of BDSF, whereas deletion of Bcam0581 abrogated the inhibitory ability of the bacterial pathogen. (pubfacts.com)
- The bacterial factors involved in B. cenocepacia intracellular survival are not fully elucidated. (microbiologyresearch.org)
Complex6
- Synthetic cystic fibrosis sputum medium (SCFM) is rich in amino acids and supports robust growth of Burkholderia cenocepacia, a member of the Burkholderia cepacia complex (Bcc). (umanitoba.ca)
- Results: Efficient recombinant protein production and crystallization protocols were developed, and high-resolution crystal structures of IspF from P. falciparum ( Pf IspF) and B. cenocepacia ( Bc IspF) in complex with cytidine nucleotides determined. (edu.pl)
- Draft Genome of Burkholderia cenocepacia TAtl-371, a Strain from the Burkholderia cepacia Complex Retains Antagonism in Different Carbon and Nitrogen Sources. (bvsalud.org)
- Speert DP , Henry DA , Vandamme P , Corey M , Mahenthiralingam E . Epidemiology of Burkholderia cepacia complex in patients with cystic fibrosis, Canada. (cdc.gov)
- Taxonomy and identification of the Burkholderia cepacia complex. (cdc.gov)
- The impact of Burkholderia cepacia complex on morbidity in the Belgian cystic fibrosis population is high and not limited to Burkholderia cenocepacia . (ersjournals.com)
Sequence2
- 5ixh is a 2 chain structure with sequence from Burkholderia cenocepacia bc7 . (proteopedia.org)
- RT "Complete sequence of chromosome 2 of Burkholderia cenocepacia MC0- RT 3. (univ-lyon1.fr)
Cystic fibrosis lung2
- These results suggest that salicylate is a natural substrate for the efflux pump in B. cenocepacia and imply that the environment of low iron concentration in the cystic fibrosis lung can induce efflux-mediated resistance, even in the absence of antibiotic selective pressure. (jci.org)
- Focusing on a pathogen of the cystic fibrosis lung, Burkholderia cenocepacia, we sequenced clones and metagenomes to unravel the mutations and evolutionary forces responsible for adaptation and diversification of a single biofilm community during 1,050 generations of selection. (pnas.org)
Elegans4
- In this work, we aimed to determine the relevance of PaaK1 activity to the killing ability of B. cenocepacia to C. elegans . (frontiersin.org)
- We also demonstrate that the intracellular c-di-GMP level determines the virulence of B. cenocepacia to Caenorhabditis elegans and Galleria mellonella. (unibas.ch)
- Um Virulenzfaktoren zu identifizieren, die nicht durch das QS-System reguliert sind, wurde die Pathogenität von ca. 1.000 B. cenocepacia Tn5-Insertionsmutanten im C. elegans Modell untersucht. (tum.de)
- Insgesamt wurde gezeigt, dass einzelne Virulenzfaktoren von B. cenocepacia in C. elegans keine (Protease- und Lipase-Aktivität sowie EPS-Produktion) oder nur eine geringe Rolle (Siderophore) spielen und dass die Pathogenität dieses Bakteriums durch ein komplexes Zusammenwirken verschiedener Faktoren und Mechanismen reguliert ist, die erst zum Teil aufgeklärt sind. (tum.de)
Antibiotic1
- B. cenocepacia is intrinsically resistant to antibiotic therapy and often impossible to eradicate from lungs of infected CF patients [ 9 ]. (biomedcentral.com)
Mutants2
- A transcriptomic analysis of RND-4 and RND-9 (BCAM1945 to BCAM1947) deletion mutants revealed that in B. cenocepacia , RND pumps also influence other phenotypic traits important for pathogenesis ( 9 ). (asm.org)
- Deletion of the lxa locus resulted in B. cenocepacia mutants with aerobic growth deficiencies in minimal medium and compromised viability after prolonged incubation in the absence of oxygen. (cf.ac.uk)
Mutation3
- Here, we present a genome-wide view of the molecular mutation spectrum in Burkholderia cenocepacia , a clinically relevant pathogen with high %GC content and multiple chromosomes. (genetics.org)
- We find that B. cenocepacia has low genome-wide mutation rates with insertion-deletion mutations biased toward deletions, consistent with the idea that deletion pressure reduces prokaryotic genome sizes. (genetics.org)
- Unlike prior studies of other organisms, mutations in B. cenocepacia are not AT biased, which suggests that at least some genomes with high %GC content experience unusual base-substitution mutation pressure. (genetics.org)
Lung1
- We specifically demonstrate that undefined rich media leads to higher susceptibility of B. cenocepacia to P. aeruginosa toxins like cyanide as compared to a synthetic medium (SCFM), that mimics the CF lung nutritional content. (dartmouth.edu)
Identification1
- In addition, we reported on the identification of lifestyle-specific efflux pumps involved in B. cenocepacia tolerance to the disinfectant chlorhexidine (CHX) ( 12 ). (asm.org)
Draft genome2
- We sequenced the draft genome of Burkholderia cenocepacia KC-01, isolated from the coastal saline soil, and identified several potential sRNAs in silico . (microbiologyresearch.org)
- Here, we report and describe the improved, high- quality permanent draft genome of B. cenocepacia TAtl-371, which was sequenced using a combination of PacBio RS and PacBio RS II sequencing methods . (bvsalud.org)
Genomic islands1
- The distribution of internal transcription start sites can be used to identify genomic islands in B. cenocepacia. (nih.gov)
Locus2
- In summary, transcriptomic profiling of B. cenocepacia revealed an unexpected ability of aerobic Burkholderia to persist in the absence of oxygen and identified the novel lxa locus as key determinant of this important ecophysiological trait. (cf.ac.uk)
- Attachment was dramatically reduced in a B. cenocepacia K56-2Δlxa-locus deletion mutant, further indicating that it encodes protein(s) involved in host-cell attachment. (maynoothuniversity.ie)
Protein3
- Here we present crystal structures of Burkholderia cenocepacia haem importer BhuUV complexed with the periplasmic haem-binding protein BhuT and in the absence of BhuT. (rcsb.org)
- Furthermore, proteomic analysis utilizing iTRAQ were used to study the protein expression of B. cenocepacia K56-2 grown in the amino acid-rich SCFM. (umanitoba.ca)
- Interestingly, our results also showed the over-expression of flagellin and membrane efflux protein which are involved in the virulence of B. cenocepacia. (umanitoba.ca)
Virulence factors1
- Jointly, these experiments indicate that B. cenocepacia express virulence factors capable of interfering with Rab7 function and thereby with membrane traffic. (qub.ac.uk)
Resistance2
- Multidrug resistance in B. cenocepacia can be ascribed to different mechanisms, including the activity of efflux pumps and biofilm formation. (asm.org)
- Recently, we showed that the RND-4 efflux system is involved in the resistance to a new thiopyridine drug effective against B. cenocepacia ( 11 ). (asm.org)
Patients5
- B. cenocepacia is an opportunistic human pathogen that is particularly problematic for patients suffering from cystic fibrosis. (omicsdi.org)
- B. cenocepacia bacteraemia in two patients in the Canberra Hospital ICU prompted an investigation into a common source. (acipcconference.com.au)
- Burkholderia cenocepacia infects patients with cystic fibrosis. (microbiologyresearch.org)
- Burkholderia cenocepacia is a Gram-negative opportunistic pathogen of patients with cystic fibrosis and chronic granulomatous disease. (qxmd.com)
- Similalrly, it was reported that B. cenocepacia strain PHDC recovered from most CF patients in the mid-Atlantic region of the USA could also be isolated from agricultural soils in the same region (32). (apsnet.org)