A species of gram-negative bacteria that causes disease in plants. It is found commonly in the environment and is an opportunistic pathogen in humans.
A genus of gram-negative, aerobic, rod-shaped bacteria. Organisms in this genus had originally been classified as members of the PSEUDOMONAS genus but overwhelming biochemical and chemical findings indicated the need to separate them from other Pseudomonas species, and hence, this new genus was created.
A group of phenotypically similar but genotypically distinct species (genomovars) in the genus BURKHOLDERIA. They are found in water, soil, and the rhizosphere of crop plants. They can act as opportunistic human pathogens and as plant growth promoting and biocontrol agents.
Infections with bacteria of the genus BURKHOLDERIA.
A species of BURKHOLDERIA considered to be an opportunistic human pathogen. It has been associated with various types of infections of nosocomial origin.
A species of gram-negative, aerobic bacteria that causes MELIOIDOSIS. It has been isolated from soil and water in tropical regions, particularly Southeast Asia.
A phenomenon where microorganisms communicate and coordinate their behavior by the accumulation of signaling molecules. A reaction occurs when a substance accumulates to a sufficient concentration. This is most commonly seen in bacteria.
An autosomal recessive genetic disease of the EXOCRINE GLANDS. It is caused by mutations in the gene encoding the CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR expressed in several organs including the LUNG, the PANCREAS, the BILIARY SYSTEM, and the SWEAT GLANDS. Cystic fibrosis is characterized by epithelial secretory dysfunction associated with ductal obstruction resulting in AIRWAY OBSTRUCTION; chronic RESPIRATORY INFECTIONS; PANCREATIC INSUFFICIENCY; maldigestion; salt depletion; and HEAT PROSTRATION.
Herbaceous biennial plants and their edible bulbs, belonging to the Liliaceae.
A disease of humans and animals that resembles GLANDERS. It is caused by BURKHOLDERIA PSEUDOMALLEI and may range from a dormant infection to a condition that causes multiple abscesses, pneumonia, and bacteremia.
Cyclic esters of acylated BUTYRIC ACID containing four carbons in the ring.
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
Encrustations, formed from microbes (bacteria, algae, fungi, plankton, or protozoa) embedding in extracellular polymers, that adhere to surfaces such as teeth (DENTAL DEPOSITS); PROSTHESES AND IMPLANTS; and catheters. Biofilms are prevented from forming by treating surfaces with DENTIFRICES; DISINFECTANTS; ANTI-INFECTIVE AGENTS; and antifouling agents.
A sulfanilamide that is used as an antibacterial agent.
A mitosporic Ceratobasidiaceae fungal genus that is an important plant pathogen affecting potatoes and other plants. There are numerous teleomorphs.
Proteins found in any species of bacterium.
The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.
Ability of a microbe to survive under given conditions. This can also be related to a colony's ability to replicate.
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
A species of gram-negative, aerobic bacteria that acts as both a human and plant pathogen.
Inflammation of the lung parenchyma that is associated with BRONCHITIS, usually involving lobular areas from TERMINAL BRONCHIOLES to the PULMONARY ALVEOLI. The affected areas become filled with exudate that forms consolidated patches.
The functional hereditary units of BACTERIA.
A mixture of polymyxins B1 and B2, obtained from Bacillus polymyxa strains. They are basic polypeptides of about eight amino acids and have cationic detergent action on cell membranes. Polymyxin B is used for infections with gram-negative organisms, but may be neurotoxic and nephrotoxic.
One of the FURANS with a carbonyl thereby forming a cyclic lactone. It is an endogenous compound made from gamma-aminobutyrate and is the precursor of gamma-hydroxybutyrate. It is also used as a pharmacological agent and solvent.
A contagious disease of horses that can be transmitted to humans. It is caused by BURKHOLDERIA MALLEI and characterized by ulceration of the respiratory mucosa and an eruption of nodules on the skin.
Fatty acids which are unsaturated in only one position.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
The ability of bacteria to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The genetic complement of a BACTERIA as represented in its DNA.
Those components of an organism that determine its capacity to cause disease but are not required for its viability per se. Two classes have been characterized: TOXINS, BIOLOGICAL and surface adhesion molecules that effect the ability of the microorganism to invade and colonize a host. (From Davis et al., Microbiology, 4th ed. p486)
Substances that reduce the growth or reproduction of BACTERIA.
A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.
Membrane-bound cytoplasmic vesicles formed by invagination of phagocytized material. They fuse with lysosomes to form phagolysosomes in which the hydrolytic enzymes of the lysosome digest the phagocytized material.
Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
Electron microscopy in which the ELECTRONS or their reaction products that pass down through the specimen are imaged below the plane of the specimen.
Material coughed up from the lungs and expectorated via the mouth. It contains MUCUS, cellular debris, and microorganisms. It may also contain blood or pus.
In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.
A metabolic process that converts GLUCOSE into two molecules of PYRUVIC ACID through a series of enzymatic reactions. Energy generated by this process is conserved in two molecules of ATP. Glycolysis is the universal catabolic pathway for glucose, free glucose, or glucose derived from complex CARBOHYDRATES, such as GLYCOGEN and STARCH.
Biosynthesis of GLUCOSE from nonhexose or non-carbohydrate precursors, such as LACTATE; PYRUVATE; ALANINE; and GLYCEROL.
The simplest saturated hydrocarbon. It is a colorless, flammable gas, slightly soluble in water. It is one of the chief constituents of natural gas and is formed in the decomposition of organic matter. (Grant & Hackh's Chemical Dictionary, 5th ed)
A sulfhydryl compound used to prevent urothelial toxicity by inactivating metabolites from ANTINEOPLASTIC AGENTS, such as IFOSFAMIDE or CYCLOPHOSPHAMIDE.
A genus of anaerobic, irregular spheroid-shaped METHANOSARCINALES whose organisms are nonmotile. Endospores are not formed. These archaea derive energy via formation of methane from acetate, methanol, mono-, di-, and trimethylamine, and possibly, carbon monoxide. Organisms are isolated from freshwater and marine environments.
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
An enzyme that catalyzes the conversion of urea and water to carbon dioxide and ammonia. EC 3.5.1.5.
A compound formed in the liver from ammonia produced by the deamination of amino acids. It is the principal end product of protein catabolism and constitutes about one half of the total urinary solids.
A type of scanning probe microscopy in which a probe systematically rides across the surface of a sample being scanned in a raster pattern. The vertical position is recorded as a spring attached to the probe rises and falls in response to peaks and valleys on the surface. These deflections produce a topographic map of the sample.
Cell-surface components or appendages of bacteria that facilitate adhesion (BACTERIAL ADHESION) to other cells or to inanimate surfaces. Most fimbriae (FIMBRIAE, BACTERIAL) of gram-negative bacteria function as adhesins, but in many cases it is a minor subunit protein at the tip of the fimbriae that is the actual adhesin. In gram-positive bacteria, a protein or polysaccharide surface layer serves as the specific adhesin. What is sometimes called polymeric adhesin (BIOFILMS) is distinct from protein adhesin.

BcsKC is an essential protein for the type VI secretion system activity in Burkholderia cenocepacia that forms an outer membrane complex with BcsLB. (1/74)

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Ecological succession in long-term experimentally evolved biofilms produces synergistic communities. (2/74)

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Inactivation of macrophage Rab7 by Burkholderia cenocepacia. (3/74)

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The pobA gene of Burkholderia cenocepacia encodes a group I Sfp-type phosphopantetheinyltransferase required for biosynthesis of the siderophores ornibactin and pyochelin. (4/74)

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The Burkholderia cenocepacia LysR-type transcriptional regulator ShvR influences expression of quorum-sensing, protease, type II secretion, and afc genes. (5/74)

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Virulence of an emerging respiratory pathogen, genus Pandoraea, in vivo and its interactions with lung epithelial cells. (6/74)

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Burkholderia cenocepacia O polysaccharide chain contributes to caspase-1-dependent IL-1beta production in macrophages. (7/74)

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CD4+ T-cell immunity to the Burkholderia pseudomallei ABC transporter LolC in melioidosis. (8/74)

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Burkholderia cepacia has emerged during the past 2 decades as an important opportunistic pathogen in persons with cystic fibrosis (CF) (11). Although B. cepacia was previously regarded as a single species, recent taxonomic studies have identified at least nine closely related species in what is now referred to as the B. cepacia complex (Bcc). This group includes B. cepacia, Burkholderia multivorans, Burkholderia cenocepacia, Burkholderia stabilis, Burkholderia vietnamiensis, Burkholderia dolosa, Burkholderia ambifaria, Burkholderia anthina, and Burkholderia pyrrocinia (4-6, 25, 28-32). Although each of these species has been recovered from respiratory secretions of persons with CF, B. multivorans and B. cenocepacia account for the great majority of isolates (12, 24). Mounting evidence also suggests that B. cenocepacia is somewhat more pathogenic in CF than are the remaining species (1, 8).. A feature that distinguishes Bcc from other pathogens in CF is the occurrence in a significant proportion ...
In a previous study, we identified a gene, BCAM0224, encoding a trimeric autotransporter adhesin (TAA), which appears to be restricted to epidemic-associated B. cenocepacia strains of the ET-12 lineage (22). Transcriptional analysis revealed that BCAM0224 is located within a bicistronic operon composed of 9 genes, which comprises, among others, two additional TAA-encoding genes (BCAM0219 and BCAM0223) (21). In an attempt to characterize the functions of the TAA-encoding genes in B. cenocepacia, we have generated mutations and analyzed the associated phenotypes. Based on this, we previously proposed that the BCAM0224 protein is involved in virulence, since the BCAM0224 mutant was less virulent than the wild-type strain in an insect infection model (22). In addition, disruption of the BCAM0223 gene impairs the ability of the mutant strain to adhere to epithelial cells, decreases serum resistance, and reduces virulence (21). Finally, in a recent report, we used atomic force microscopy (AFM) to ...
TY - JOUR. T1 - A Decade of Burkholderia cenocepacia Virulence Determinant Research. AU - Loutet, S.A.. AU - Valvano, Miguel. PY - 2010/10. Y1 - 2010/10. N2 - The Burkholderia cepacia complex (Bcc) is a group of genetically related environmental bacteria that can cause chronic opportunistic infections in patients with cystic fibrosis (CF) and other underlying diseases. These infections are difficult to treat due to the inherent resistance of the bacteria to antibiotics. Bacteria can spread between CF patients through social contact and sometimes cause cepacia syndrome, a fatal pneumonia accompanied by septicemia. Burkholderia cenocepacia has been the focus of attention because initially it was the most common Bcc species isolated from patients with CF in North America and Europe. Today, B. cenocepacia, along with Burkholderia multivorans, is the most prevalent Bcc species in patients with CF. Given the progress that has been made in our understanding of B. cenocepacia over the past decade, we ...
Mapping transcription start sites across the B. cenocepacia genome added relevant information to the J2315 annotation. Genes and novel regulatory RNAs putatively involved in B. cenocepacia biofilm formation were identified. These findings will help in understanding regulation of B. cenocepacia biofi …
The cciIR quorum-sensing system associated with epidemic strains of B. cenocepacia is involved in the regulation of known virulence factors and is a part of a regulatory network with the cepIR quorum-sensing system, since CepR is required for cciIR expression. Phylogenetic analysis demonstrated that the cepIR and cciIR systems are highly distinct. The cepIR system that is widely distributed among the Bcc was predicted to be the ancestral system, whereas the cciIR system was likely acquired by horizontal gene transfer when cci was incorporated into the B. cenocepacia genome. The presence of an ancestral and a more-recently acquired AHL-dependent quorum-sensing system has been observed in other species. The P. aeruginosa lasIR system is considered to be the ancestral system, and the rhlIR system is considered to be more recently acquired (22). The rhlIR system has been incorporated into the regulatory pathway of the lasIR system and is dependent on the lasIR system for expression (21, 34). Both of ...
Burkholderia cenocepacia ATCC ® BAA-245D-5™ Designation: Genomic DNA from Burkholderia cenocepacia strain LMG 16656 TypeStrain=False Application:
Methane is metabolized principally by methanotrophs and methanogens in the global carbon cycle. Methanotrophs consume methane as the only source of carbon, while methanogens produce methane as a metabolic byproduct. Methylotrophs, which are microorganisms that can obtain energy for growth by oxidizing one-carbon compounds, such as methanol and methane, are situated between methanotrophs and methanogens. Methanogens can obtain energy for growth by converting a limited number of substrates to methane under anaerobic conditions. Three types of methanogenic pathways are known: CO2 to methane [MD:M00567], methanol to methane [MD:M00356], and acetate to methane [MD:M00357]. Methanogens use 2-mercaptoethanesulfonate (CoM; coenzyme M) as the terminal methyl carrier in methanogenesis and have four enzymes for CoM biosynthesis [MD:M00358]. Coenzyme B-Coenzyme M heterodisulfide reductase (Hdr), requiring for the final reaction steps of methanogenic pathway, is divided into two types: cytoplasmic HdrABC in ...
Glycolysis is the process of converting glucose into pyruvate and generating small amounts of ATP (energy) and NADH (reducing power). It is a central pathway that produces important precursor metabolites: six-carbon compounds of glucose-6P and fructose-6P and three-carbon compounds of glycerone-P, glyceraldehyde-3P, glycerate-3P, phosphoenolpyruvate, and pyruvate [MD:M00001]. Acetyl-CoA, another important precursor metabolite, is produced by oxidative decarboxylation of pyruvate [MD:M00307]. When the enzyme genes of this pathway are examined in completely sequenced genomes, the reaction steps of three-carbon compounds from glycerone-P to pyruvate form a conserved core module [MD:M00002], which is found in almost all organisms and which sometimes contains operon structures in bacterial genomes. Gluconeogenesis is a synthesis pathway of glucose from noncarbohydrate precursors. It is essentially a reversal of glycolysis with minor variations of alternative paths [MD:M00003 ...
Burkholderia cenocepacia is a Gram-negative aerobic bacterium that belongs to a group of opportunistic pathogens displaying diverse environmental and pathogenic lifestyles. B. cenocepacia is known for its ability to cause lung infections in people with cystic fibrosis and it possesses a large 8 Mb multireplicon genome encoding a wide array of pathogenicity and fitness genes. Transcriptomic profiling across nine growth conditions was performed to identify the global gene expression changes made when B. cenocepacia changes niches from an environmental lifestyle to infection. In comparison to exponential growth, the results demonstrated that B. cenocepacia changes expression of over one-quarter of its genome during conditions of growth arrest, stationary phase and surprisingly, under reduced oxygen concentrations (6% instead of 20.9% normal atmospheric conditions). Multiple virulence factors are upregulated during these growth arrest conditions. A unique discovery from the comparative expression ...
Burkholderia cenocepacia is an opportunistic pathogen causing life-threatening infections in cystic fibrosis and other immunocompromised patients. The bacterium survives within macrophages by interfering with typical endocytic trafficking, resulting in delayed maturation of a B. cenocepacia-containing phagosome. We hypothesize that B. cenocepacia alters gene expression after internalization by macrophages, inducing genes involved in intracellular survival and host adaptation. Furthermore, we hypothesize that specialized bacterial secretion systems are involved in the interactions between intracellular bacteria and macrophages. In this work, we characterize later-stage infection of macrophages by B. cenocepacia, showing replication within an acidified endosomal compartment suggestive of a phagolysosome. We examine differential gene expression by intracellular B. cenocepacia using selective capture of transcribed sequences (SCOTS) with both competitive enrichment and microarray analysis. We identified 766
Since bceU is found in the genome sequences of all Bcc strains as well as in B. thailandensis, B. oklahomensis, and B. pseudomallei strains, the authors of PMID:19948863 considered it to belong as part of bce-II gene cluster ...
Dr Heather Wilson1, Dr Karina Kennedy1, Ms Shannon Broadrick1, Ms Fiona Kimber1, Ms Wendy Beckingham1, Prof Peter Collignon1. 1Canberra Hospital And Health Services, Canberra, Australia. Introduction:. Burkholderia cenocepacia is an environmental organism and an unusual isolate from blood, although is a recognised cause of nosocomial outbreaks due to contamination of various fluids. B. cenocepacia bacteraemia in two patients in the Canberra Hospital ICU prompted an investigation into a common source.. Methods:. Clinical and epidemiological data were obtained from the patients medical records. Clinical and environmental specimens were cultured and identified by MALDI-TOF (Bruker). Further characterisation, using recA gene sequencing and whole genome sequencing, was performed at Queensland Pathology.. Results:. The finding that our two patients occupied the same bed within hours of having the blood cultures collected directed environmental testing of the area. B. cepacia complex was grown from a ...
Purchase Recombinant Burkholderia cenocepacia ATP-dependent Clp protease adapter protein ClpS(clpS). It is produced in Yeast. High purity. Good price.
Please Note: Ortholuge cannot be used for certain RBB analyses involving closely-related strains (e.g. Burkholderia cenocepacia J2315 versus Burkholderia cenocepacia AU 1054) because the phylogenetic distance between each ingroup protein is very small in relation to the phylogenetic distance between each ingroup protein and the associated outgroup protein. In these cases, the Ortholuge Classification value will be left blank which indicates Ortholuge analysis was not peformed, however the two proteins may very well be orthologs. In other cases, only some rows will be missing Ortholuge classifications, which can occur when a suitable gene could not be found in the outgroup species ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides.
Scientists may have found a way to kill Burkholderia cenocepacia, a multi-drug resistant plant pathogen that causes severe infections in people with cystic fibrosis.. The team of researchers has identified a weakness in the armour that protects the B. cenocepacia bacterium from the effects of antibiotics. They hypothesize that preventing the synthesis of a key sugar required for this armour, 4-amino-4-deoxy-L-arabinose (Ara4N), may lead to a susceptibility within the cell membrane to antibiotics. Researchers hope to discover novel molecules as antibiotic candidates which could disrupt the synthesis of Ara4N.. ...
SWISS-MODEL Repository entry for A0K968 (Y2294_BURCH), Probable transcriptional regulatory protein Bcen2424_2294. Burkholderia cenocepacia (strain HI2424)
SWISS-MODEL Repository entry for A0K923 (NUOA_BURCH), NADH-quinone oxidoreductase subunit A. Burkholderia cenocepacia (strain HI2424)
Joseph D. Mougous and coworkers at the University of Washington School of Medicine and HHMI discovered a cytidine deaminase, which converts cytosine (C) to uracil (U), in the bacteria Burkholderia cenocepacia and thought the enzyme could be useful for genome editing. Mougous reached out to David R. Liu at the Broad Institute, Harvard University, and HHMI, because Lius group had previously developed base editors, which are engineered proteins that make specific single-letter changes in DNA.. ...
We report a case of a vaginal infection caused by a strain of Burkholderia cenocepacia. The strain was isolated from vaginal swab specimens from a 68-year-old woman with smoldering myeloma and chronic hepatitis C virus infection who was hospitalized ...
4B6Z: High-Resolution Structure of the M14-Type Cytosolic Carboxypeptidase from Burkholderia Cenocepacia Refined Exploiting Pdb_Redo Strategies.
Zaradi pogoste uporabe betalaktamskih zdravil so nekatere bakterije razvile odpornost proti njim. Nekatere bakterije proizvajajo prej omejeni encim betalaktamazo, ki izniči učinek betalaktamskega antibiotika, ker cepi betalaktamski obroč.. Za preprečitev tovrstne odpornosti bakterij so na trgu kombinacije betalaktamskih antibiotikov z zaviralci betalaktamaze. Takšna kombinacija je npr. amoksicilin + klavulanska kislina. Klavulanska kislina je zaviralec betalaktamaze, ker se nepovratno veže na encim in slednji se več ne more vezati na betalaktamsko učinkovino (v tem primeru amoksicilin).. ...
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The phenylacetic acid (PA) degradative pathway is the central pathway by which complex aromatic compounds (e.g. styrene) get degraded. Upper pathways for different aromatic compounds converge at common intermediate phenylacetyl-CoA, which is then metabolized to succinyl-CoA and acetyl-CoA. We previously made a link in Burkholderia cenocepacia between phenylacetic acid (PA) degradation and virulence by showing that insertional mutagenesis of paaA and paaE results in PA-conditional growth and an attenuated killing phenotype in the Caenorhabditis elegans model of infection. Insertional mutagenesis of paaK1, which encodes a phenylacetyl-CoA ligase, did not result in a PA-conditional growth probably due to the presence of a second similar gene (paaK2) encoding another phenylacetyl-CoA ligase (PaaK2). Recently published crystallographic and enzyme kinetics studies comparing the two ligases show that PaaK1 is better able to bind hydroxylated PA derived molecules such as 3-hydroxyphenylacetic (3-OHPA) acid and
Essential gene studies often reveal novel essential functions for genes with dispensable homologues in other species. This is the case with the widespread family of electron transfer flavoproteins (ETFs), which are required for the metabolism of specific substrates or for symbiotic nitrogen fixation in some bacteria. Despite these non-essential functions high-throughput screens have identified ETFs as putatively essential in several species. In this study, we constructed a conditional expression mutant of one of the ETFs in Burkholderia cenocepacia, and demonstrated that its expression is essential for growth on both complex media and a variety of single-carbon sources. We further demonstrated that the two subunits EtfA and EtfB interact with each other, and that cells depleted of ETF are non-viable and lack redox potential. These cells also transition from the short rods characteristic of Burkholderia cenocepacia to small spheres independently of MreB. The putative membrane partner ETF dehydrogenase
O -linked protein glycosylation is a conserved feature of the Burkholderia genus. For Burkholderia cenocepacia , the addition of the trisaccharide β-G
Acylhomoserine lactone (AHL) analogues in which the amide function is replaced by a triazole group were synthesized and evaluated for their effect on quorum sensing (QS) and biofilm formation in Burkholderia cenocepacia and Pseudomonas aeruginosa. In addition, the influence of the length of the acyl-mimicking chain was investigated. The compounds showed selectivity between two different AHL QS systems. 3-(1H-1,2,3-Triazol-1-yl)dihydrofuran-2(3H)-ones, in which the 4-substituent best resembled the acyl chain of the native AHL molecule exhibited significant QS agonistic and antagonistic activities. Replacing this aliphatic substituent by a phenyl-containing moiety resulted in active inhibitors of QS. The most active compounds showed biofilm inhibitory as well as biofilm eradicating activities in both test organisms. ...
DNA microarrays have proven powerful for functional genomics studies. Several technologies exist for the generation of whole-genome arrays. It is well documented that 25mer probes directed against different regions of the same gene produce variable signal intensity values. However, the extent to which this is true for probes of greater length (60mers) is not well characterized. Moreover, this information has not previously been reported for whole-genome arrays designed against bacteria, whose genomes may differ substantially in characteristics directly affecting microarray performance. We report here an analysis of alternative 60mer probe designs for an in-situ synthesized oligonucleotide array for the GC rich, β-proteobacterium Burkholderia cenocepacia. Probes were designed using the ArrayOligoSel3.5 software package and whole-genome microarrays synthesized by Agilent, Inc. using their in-situ, ink-jet technology platform. We first validated the quality of the microarrays as demonstrated by an average
Survival curves for D. melanogaster infected with B. cenocepacia strains.Pricking assays were performed with a minimum of 30 flies for each strain. Statistical
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TY - CONF. T1 - Comparison of biofilm formation between Burkholderia multivorans Burkholderia cenocepacia Pseudomonas aeruginosa in the presence or absence of mucin. AU - Einarsson, Gisli. AU - McDowell, Andrew. AU - Elborn, Joseph. AU - Martin, Lorraine. PY - 2009/6. Y1 - 2009/6. M3 - Paper. SP - S34-S34. T2 - European Cystic Fibrosis Conference. Y2 - 1 June 2009 through 1 June 2009. ER - ...
Aabed, Tariq Ahmad (2018) Radiation Effects on Mesenchymal Stem Cells in a Model of Fibrosarcoma. PhD thesis, University of Sheffield. Albaz, Alrayan (2018) Investigation into Cytosine Epigenetic State in Bladder Cancer. PhD thesis, University of Sheffield. Aljadani, Saeedah (2018) Regulation of iron acquisition by an iron starvation sigma factor system in Burkholderia cenocepacia. PhD thesis, University of Sheffield. Alkathiri, Afnan (2018) Investigation the Role of ATM in Sarcomas. PhD thesis, University of Sheffield. Allocca, Gloria (2018) Defining the perivascular niche in the early stages of breast cancer bone metastasis. PhD thesis, University of Sheffield. Almaghrabi, Sarah (2018) Gene Therapy for an Experimental Autoimmune Polyglandular Syndrome 1 Model Caused by Mutations in Aire. PhD thesis, University of Sheffield. Alrahimi, Jehan (2018) The Role of Tetraspanins in Pseudomonas aeruginosa Adherence to Human Cells. PhD thesis, University of Sheffield. Alshamrani, Khalaf (2018) ...
This pipeline reflects, to the best of our knowledge, publicly available information on the status of vaccine candidates with a direction towards human use for Lassa and Nipah (as at August 2017) and for MERS-CoV (as at July 2017). Please contact [email protected] if you have evidence to report on updates to this.. ...
Össur is a trusted supplier of the NHS, having supplied supports and braces for medical and injury supports for over 10 years. Free standard delivery on all orders.
Zaviralci proteaz ali proteazni zaviralci (oznaka ATC: J05AE) so skupina učinkovin za zdravljenje ali preprečevanje virusnih okužb, vključno z okužbami z virusom HIV in virusom hepatitisa C. Preprečujejo virusno podvojevanje, tako da zavrejo delovanje encima proteaze (npr. proteaze HIV-1, ki cepi nastajajoče beljakovine, ki se nato združujejo in tvorijo virione). Doslej so razvili oziroma so v fazi razvoja proteazni zaviralci za naslednje virusne okužbe:: ...
General Information: Burkholderia cenocepacia MC0-3 was isolated from soil associated with maize roots. This species (genomovar III) is part of the Burkholderia cepacia complex, which consists of 9 closely related species, also known as genomovars. These organisms are commonly found in soil and all are opportunistic pathogens espcially in cystic fibrosis patients. ...
Chronic pulmonary infections are the principal cause of morbidity and mortality in individuals with cystic fibrosis (CF). Due to the polymicrobial nature of these infections, the identification of the particular bacterial species responsible is an essential step in diagnosis and treatment. Current diagnostic procedures are time-consuming, and can also be expensive, invasive and unpleasant in the absence of spontaneously expectorated sputum. The development of a rapid, non-invasive methodology capable of diagnosing and monitoring early bacterial infection is desired. Future visions of real-time, in situ diagnosis via exhaled breath testing rely on the differentiation of bacteria based on their volatile metabolites. The objective of this proof-of-concept study was to investigate whether a range of CF-associated bacterial species (i.e. Pseudomonas aeruginosa, Burkholderia cenocepacia, Haemophilus influenzae, Stenotrophomonas maltophilia, Streptococcus pneumoniae and Streptococcus milleri) could be ...
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Ah, yes! Im dealing with Burkholderia. 16s rDNA analyses showed similarity to B. cepacia and B. cenocepacia. Perhaps the fact that B. cepacia exists as a complex of many strains (genomovars) that makes biochemical identification inaccurate; what more when I did it the conventional way. Hoping this time that the commercial kit like API could back the 16s analyses up.. ...
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The Burkholderia cepacia complex consists of nine closely related species: Burkholderia cepacia genomovars I and VI, Burkholderia multivorans (genomovar II), Burkholderia cenocepacia (genomovar III), Burkholderia stabilis (genomovar IV), Burkholderia vietnamiensis (genomovar V), Burkholderia ambifaria (genomovar VII), Burkholderia anthina (genomovar VIII), and Burkholderia pyrrocinia (genomovar IX) (2, 9, 10). Despite the advances that have been made in the understanding of the taxonomy and epidemiology of this group, infections of the respiratory tract with B. cepacia complex organisms still have a considerable impact on morbidity and mortality in cystic fibrosis (CF) patients (4, 5, 6). B. cepacia complex strains have also attracted attention as possible biocontrol and bioremediation agents that could be used as antagonists of soil-borne plant pathogens, as plant growth-promoting agents, and for the bioremediation of various recalcitrant xenobiotics (3, 8). To aid in identification, ...
This study aimed firstly to establish the distribution and copy number within the Burkholderia cepacia complex of three insertion sequences (IS402, IS407 and IS1416) that possess the ability to activate transcription and hence influence gene expression. A second aim was to map the genomic insertion sites of one of the active insertion sequences (IS407) to establish putative links between insertion site and downstream gene activation. The resulting data revealed that all three insertion sequences were present in one-third of the 66 isolates tested. The three insertion sequences were prevalent across the nine B. cepacia complex species, although IS402 was absent from the 16 Burkholderia anthina strains tested and IS407 was absent from all 10 Burkholderia pyrrocinia strains. IS407 copies from six strains (two Burkholderia cenocepacia strains and one strain each of Burkholderia multivorans, Burkholderia stabilis, Burkholderia vietnamiensis and B. anthina) were mapped to the genome using hemi-nested inverse
Bacteria of the Burkholderia cepacia complex (Bcc) are pathogens of humans, plants, and animals. Burkholderia cenocepacia is one of the most common Bcc species infecting cystic fibrosis (CF) patients and its carriage is associated with poor prognosis. In this study, we characterized a general O-link …
A plate assay to detect bacterial lipase (EC 3.1.1.3) in a medium containing trioleoylglycerol and the fluorescent dye rhodamine B is presented. Substrate hydrolysis causes the formation of orange fluorescent halos around bacterial colonies visible upon UV irradiation. The logarithm of lipase activity from cell-free culture supernatants is linearly correlated with the diameter of halos, thereby allowing quantitation of lipase activities ranging from 1 to 30 nkat. ...
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The construction sector is very important for the European economy, generating almost 10% of EU GDP and providing 20 million jobs. However it has been affected by a number of adverse factors, with building and infrastructure work falling by 17% between January 2008 and April 2012 across the EU-27. Amongst the adverse factors are the bursting of the housing bubbles in several EU countries and the contraction of the market for credit. As well as addressing these problems it is seen as important to ensure the sustainability of the sector and to meet the challenge of the introduction of nearly zero energy buildings as announced in the recast of the Energy Performance of Buildings Directive ...
Sass AM, De Waele S, Daled S, Devreese B, Deforce D, Van Nieuwerburgh F, Coenye T (October 2019). "Burkholderia cenocepacia ... Sass A, Kiekens S, Coenye T (November 2017). "Identification of small RNAs abundant in Burkholderia cenocepacia biofilms reveal ... Characterization of the secondary structures of 4 tsRNAs in Burkholderia cenocepacia suggests they form double hairpin ... Typically, each species of Burkholderia has 3-5 homologous tsRNAs. Experiments with four species of the Burkholderia lineage ...
... is produced by Burkholderia cenocepacia under iron-deficient conditions. B. cenocepacia is known to ... "The ornibactin biosynthesis and transport genes of Burkholderia cenocepacia are regulated by an extracytoplasmic function sigma ... "Ornibactin production and transport properties in strains of Burkholderia vietnamiensis and Burkholderia cepacia (formerly ... Coenye T, Vandamme P, Govan JR, LiPuma JJ (October 2001). "Taxonomy and identification of the Burkholderia cepacia complex". ...
Examples of these opportunistic pathogens include Pseudomonas aeruginosa, Burkholderia cenocepacia, and Mycobacterium avium. ...
... and motility in Burkholderia cenocepacia". Journal of Bacteriology. 193 (23): 6712-23. doi:10.1128/JB.05979-11. PMC 3232912. ... "Elucidation of the Burkholderia cenocepacia hopanoid biosynthesis pathway uncovers functions for conserved proteins in hopanoid ...
"Genomics Reveals a Unique Clone of Burkholderia cenocepacia Harboring an Actively Excising Novel Genomic Island". Frontiers in ...
... biofilms are present in the chronic Pseudomonas aeruginosa and Burkholderia cenocepacia infections characteristic of cystic ...
"The Novel Cis-Encoded Small RNA h2cR Is a Negative Regulator of hfq2 in Burkholderia cenocepacia". PLoS ONE. 7 (10): e47896. ... In molecular biology, the h2cR sRNA is a small RNA produced by species of the bacterial genus Burkholderia. It binds to the ...
As first discovered in plants, diffusible signal factors (DSFs) have been found in bacteria such as Burkholderia cenocepacia ...
... such as the chronic Pseudomonas aeruginosa and Burkholderia cenocepacia infections characteristic of cystic fibrosis. Other ...
... archaea and a number of pathogenic bacteria including Burkholderia cenocepacia, Neisseria gonorrhoeae and Acinetobacter ... "A general protein O-glycosylation system within the Burkholderia cepacia complex is involved in motility and virulence". ...
Finally, some species, such as Pseudomonas aeruginosa, Burkholderia cenocepacia, and Mycobacterium avium, are opportunistic ...
... cultures were identified as Burkholderia cenocepacia strain VIMP01 (JQ867371), Burkholderia gladioli strain VIMP02 (JQ811557), ... Burkholderia gladioli strain VIMP03 (JQ867372) and Burkholderia species strain VIMP04 (JQ867373)6]. Additionally, phosphate (P ... Isolates were strains of Burkholderia named as VIMP01, VIMP02, VIMP03 and VIMP04. VIMP (Vasantdada Sugar Institute Isolate by ...
By age 15, bacteria had colonized Smith's lungs, including a form of Burkholderia cenocepacia which mutated over a decade of ...
"Burkholderia cenocepacia". NCBI Taxonomy Browser. 95486. Type strain of Burkholderia cenocepacia at BacDive - the Bacterial ... is Burkholderia cenocepacia, a member of a bacterial group collectively referred to as the Burkholderia cepacia complex (Bcc ... Burkholderia cenocepacia is a species of Gram-negative bacteria that is common in the environment, can form a biofilm with ... sRNAs identified in Burkholderia cenocepacia KC-0 were upregulated under iron depletion and oxidate stress. In Seattle, a team ...
May 2007). "A Putative Gene Cluster for Aminoarabinose Biosynthesis Is Essential for Burkholderia cenocepacia Viability". J. ... Loutet SA, Valvano MA (October 2010). "A decade of Burkholderia cenocepacia virulence determinant research". Infect. Immun. 78 ... Burkholderia cepacia complex in the NCBI Taxonomy Browser Type strain of Burkholderia cepacia complex at BacDive, the Bacterial ... Burkholderia cepacia complex (BCC), or simply Burkholderia cepacia, is a group of catalase-producing, lactose-nonfermenting, ...
... alpina Burkholderia ambifaria Burkholderia anthina Burkholderia arboris Burkholderia cenocepacia Burkholderia ... Burkholderia dolosa Burkholderia gladioli Burkholderia glumae Burkholderia humptydooensis Burkholderia lata Burkholderia latens ... Burkholderia mallei Burkholderia metallica Burkholderia multivorans Burkholderia oklahomensis Burkholderia plantarii ... Burkholderia pseudomallei Burkholderia pseudomultivorans Burkholderia puraquae Burkholderia pyrrocinia Burkholderia seminalis ...
Burkholderia mallei Burkholderia pseudomallei Burkholderia thailandensis Burkholderia ambifaria Burkholderia cenocepacia ... Burkholderia multivorans Burkholderia vietnamiensis Burkholderia xenovorans Burkholderia sp. Burkholderia phages Pseudomonas ... Burkholderia mallei, Burkholderia pseudomallei, Clostridium perfringens, and Entamoeba histolytica) involved in new and re- ... Bacillus anthracis Bacillus cereus Bacillus thuringiensis Bacillus subtilis Bacillus pumilus Pathema-Burkholderia] ...
Burkholderia cenocepacia, na Mycobacterium avium ni ni pathojeni ambazo hutaka kutumia hali fulani ya binadamu walio na uhaba ...
Burkholderia cenocepacia. *Burkholderia gladioli. C. *Campylobacter. *Chlamydia trachomatis. *Chlamydiae. *Chlorobi. * ...
Biofilm dapat ditemui pada banyak infeksi, seperti infeksi Pseudomonas aeruginosa kronis dan Burkholderia cenocepacia, yang ...
Nekatere bakterije (uspešne trajne okužbe - kronična Pseudomonas Aeruginosa in Burkholderia cenocepacia, značilne za cistično ...
"Burkholderia cenocepacia". NCBI Taxonomy Browser. 95486. Type strain of Burkholderia cenocepacia at BacDive - the Bacterial ... is Burkholderia cenocepacia, a member of a bacterial group collectively referred to as the Burkholderia cepacia complex (Bcc ... Burkholderia cenocepacia is a species of Gram-negative bacteria that is common in the environment, can form a biofilm with ... sRNAs identified in Burkholderia cenocepacia KC-0 were upregulated under iron depletion and oxidate stress. In Seattle, a team ...
Genomic DNA from Burkholderia cenocepacia strain LMG 16656 TypeStrain=False Application: ... Burkholderia cenocepacia (ATCC® BAA-245D-5™) Strain Designations: Genomic DNA from Burkholderia cenocepacia strain LMG 16656 [ ... Burkholderia cepacia (Palleroni and Holmes) Yabuuchi et al. Strain Designations Genomic DNA from Burkholderia cenocepacia ... Burkholderia cenocepacia ATCC® BAA-245D-5™ dried Total DNA: At least 5 µg in 1X TE buffer ...
Here we show that a soluble bacterial lipocalin produced by Burkholderia cenocepacia upon exposure to sublethal antibiotic ... Burkholderia cenocepacia PC184. Mutation(s): 0 Gene Names: BCPG_01022. Membrane Entity: Yes ... Here we show that a soluble bacterial lipocalin produced by Burkholderia cenocepacia upon exposure to sublethal antibiotic ... cenocepacia of lipocalin genes from Pseudomonas aeruginosa , Mycobacterium tuberculosis , and methicillin-resistant ...
Burkholderia cenocepacia sp. nov.--a new twist to an old story.. Vandamme P1, Holmes B, Coenye T, Goris J, Mahenthiralingam E, ... DNA-DNA hybridisation experiments between isolates representing Burkholderia cepacia genomovar III recA lineages IIIA and IIIB ... cenocepacia sp. nov., with LMG 16656 as the type strain, is proposed. ...
Glycolysis / Gluconeogenesis - Burkholderia cenocepacia J2315 [ Pathway menu , Organism menu , Pathway entry , Download KGML , ...
Methane metabolism - Burkholderia cenocepacia J2315 [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show ...
Genes and novel regulatory RNAs putatively involved in B. cenocepacia biofilm formation were identified. These findings will ... help in understanding regulation of B. cenocepacia biofi … ... cenocepacia genome added relevant information to the J2315 ... Genome-wide transcription start site profiling in biofilm-grown Burkholderia cenocepacia J2315 BMC Genomics. 2015 Oct 13;16:775 ... Background: Burkholderia cenocepacia is a soil-dwelling Gram-negative Betaproteobacterium with an important role as ...
sp,A0AYH4,ASSY_BURCH Argininosuccinate synthase OS=Burkholderia cenocepacia (strain HI2424) OX=331272 GN=argG PE=3 SV=1 ... Burkholderia cenocepacia (strain HI2424). ,p>This subsection of the ,a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy ... cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › ...
sp,A0K578,URE3_BURCH Urease subunit gamma OS=Burkholderia cenocepacia (strain HI2424) OX=331272 GN=ureA PE=3 SV=1 ... Burkholderia cenocepacia (strain HI2424). ,p>This subsection of the ,a href="http://www.uniprot.org/help/names_and_taxonomy_ ... cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › ...
Burkholderia cenocepacia J2315. Mutation(s): 0 Gene Names: hmuV, BCAM2630. EC: 3.6.3 (PDB Primary Data), 7.6.2 (UniProt). ... Burkholderia cenocepacia J2315. Mutation(s): 0 Gene Names: hmuU, BCAM2629. Membrane protein. Mpstruc. Group: TRANSMEMBRANE ... Find proteins for B4EKB4 (Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610)) ... Find proteins for B4EKB5 (Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610)) ...
Salicylate induces an antibiotic efflux pump in Burkholderia cepacia complex genomovar III (B. cenocepacia). ... Salicylate induces an antibiotic efflux pump in Burkholderia cepacia complex genomovar III (B. cenocepacia). ... and ciprofloxacin has been identified in Burkholderia cenocepacia (genomovar III), an important cystic fibrosis pathogen. Five ... These results suggest that salicylate is a natural substrate for the efflux pump in B. cenocepacia and imply that the ...
Burkholderia cenocepacia is a member of the B. cepacia complex, a diverse group of bacteria with important clinical ... J. J. LiPuma, and P. Vandamme, 2004 Recovery of Burkholderia cenocepacia strain PHDC from cystic fibrosis patients in Europe. ... The Rate and Molecular Spectrum of Spontaneous Mutations in the GC-Rich Multichromosome Genome of Burkholderia cenocepacia. ... The Rate and Molecular Spectrum of Spontaneous Mutations in the GC-Rich Multichromosome Genome of Burkholderia cenocepacia. ...
Burkholderia cenocepacia in cystic fibrosis: epidemiology and molecular mechanisms of virulence. Clin. Microbiol. Infect. 16: ... Genome-wide analysis of DNA repeats in Burkholderia cenocepacia J2315 identifies a novel adhesin-like gene unique to epidemic- ... Characterization of BCAM0224, a Multifunctional Trimeric Autotransporter from the Human Pathogen Burkholderia cenocepacia. ... Burkholderia cenocepacia creates an intramacrophage replication niche in zebrafish embryos, followed by bacterial dissemination ...
Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov., ... Burkholderia cenocepacia and Burkholderia multivorans: influence on survival in cystic fibrosis. Thorax 59:948-951. ... CDSs with matches identified by reciprocal FASTA with other Burkholderia species-B. cenocepacia HI2424, B. cenocepacia AU1054, ... cenocepacia island (CCI), are more frequently associated with B. cenocepacia IIIA strains than other B. cenocepacia recA ...
Burkholderia cenocepacia infection often leads to fatal cepacia syndrome in cystic fibrosis patients. However, antibiotic ... Candidate Essential Genes in Burkholderia cenocepacia J2315 Identified by Genome-Wide TraDIS Front Microbiol. 2016 Aug 22;7: ... Burkholderia cenocepacia infection often leads to fatal cepacia syndrome in cystic fibrosis patients. However, antibiotic ... cenocepacia growth under nutrient-depleted conditions. The library of B. cenocepacia mutants can subsequently be subjected to ...
Treatment with piperacillin/tazobactam eliminated B. cenocepacia infection and vaginal symptoms ... We report a case of a vaginal infection caused by a strain of Burkholderia cenocepacia. The strain was isolated from vaginal ... Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, Burkholderia cepacia genomovars I and III. J Clin ... Burkholderia anthina sp. nov. and Burkholderia pyrrocinia, two additional Burkholderia cepacia complex bacteria, may confound ...
Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Burkholderia; Burkholderia cepacia complex. ... Whole-Genome Assemblies of 56 Burkholderia Species.. Journal. Genome Announc 2:e01106-14 (2014). DOI: 10.1128/genomeA.01106-14 ...
Free resource for searching and exporting immune epitopes. Includes more than 95% of all published infectious disease, allergy, autoimmune, and transplant epitope data.
In this work, we investigated the lipopolysaccharide (LPS) of clinical variants of B. cenocepacia that were collected from a ... In this work, we investigated the lipopolysaccharide (LPS) of clinical variants of B. cenocepacia that were collected from a ... cenocepacia. The IST439 OAg biosynthetic gene cluster contains 7 of 23 genes that are closely homologous to genes found in B. ... cenocepacia. The IST439 OAg biosynthetic gene cluster contains 7 of 23 genes that are closely homologous to genes found in B. ...
We previously made a link in Burkholderia cenocepacia between phenylacetic acid (PA) degradation and virulence by showing that ... induces the phenylacetic acid degradative pathway in Burkholderia cenocepacia K56-2. Using reporter activity assays, we show ... induces the phenylacetic acid degradative pathway in Burkholderia cenocepacia K56-2. Using reporter activity assays, we show ... We previously made a link in Burkholderia cenocepacia between phenylacetic acid (PA) degradation and virulence by showing that ...
Characterization of the cciIR Quorum-Sensing System in Burkholderia cenocepacia. Rebecca J. Malott, Adam Baldwin, Eshwar ... Characterization of the cciIR Quorum-Sensing System in Burkholderia cenocepacia. Rebecca J. Malott, Adam Baldwin, Eshwar ... Characterization of the cciIR Quorum-Sensing System in Burkholderia cenocepacia. Rebecca J. Malott, Adam Baldwin, Eshwar ... Several transmissible Burkholderia cenocepacia strains that infect multiple cystic fibrosis patients contain a genomic island ...
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Identification of Essential Operons with a Rhamnose-Inducible Promoter in Burkholderia cenocepacia. Silvia T. Cardona, Carmen L ... Identification of essential genes and essential operons in B. cenocepacia K56-2.Based on the previous results, B. cenocepacia ... Identification of Essential Operons with a Rhamnose-Inducible Promoter in Burkholderia cenocepacia ... Identification of Essential Operons with a Rhamnose-Inducible Promoter in Burkholderia cenocepacia ...
Burkholderia cenocepacia) (OPCA05248) , Alias: UDP-3-O-[R-3-hydroxymyristoyl]-N-acetylglucosamine deacetylase , MW: 51.00630092 ... Burkholderia cenocepacia (strain ATCC BAA-245 / DSM 16553 / LMG 16656 / NCTC 13227 / J2315 / CF5610) (Burkholderia cepacia ( ... The genome of Burkholderia cenocepacia J2315, an epidemic pathogen of cystic fibrosis patients.. Holden M.T., Seth-Smith H.M., ...
σ54-Dependent Response to Nitrogen Limitation and Virulence in Burkholderia cenocepacia Strain H111. Martina Lardi, Claudio ... S1); consensus sequence logo for the B. cenocepacia H111 σ54-binding site (Fig. S2); growth delay of the B. cenocepacia H111 σ ... σ54-Dependent Response to Nitrogen Limitation and Virulence in Burkholderia cenocepacia Strain H111 ... σ54-Dependent Response to Nitrogen Limitation and Virulence in Burkholderia cenocepacia Strain H111 ...
A Burkholderia cenocepacia orphan LuxR homolog is involved in quorum-sensing regulation. J. Bacteriol. 191:2447-2460. ... Identification of Burkholderia cenocepacia Strain H111 Virulence Factors Using Nonmammalian Infection Hosts. Stephan Schwager, ... Burkholderia cenocepacia ZmpB is a broad-specificity zinc metalloprotease involved in virulence. Infect. Immun. 74:4083-4093. ... Burkholderia cenocepacia H111, a strain isolated from a cystic fibrosis patient, has been shown to effectively kill the ...
... cenocepacia-containing phagosome. We hypothesize that B. cenocepacia alters gene expression after internalization by ... In this work, we characterize later-stage infection of macrophages by B. cenocepacia, showing replication within an acidified ... As a whole, secretion systems appear to increase pathogenicity in macrophages while limiting the spread of B. cenocepacia ... Expression of secretion system genes is differentially-regulated by intracellular B. cenocepacia. Although none of the five ...
Quorum-sensing mutations affect attachment and stability of Burkholderia cenocepacia biofilms. Applied and Environmental ...
Time-kill analysis of a stationary phase culture of Burkholderia cenocepacia strain LMG 16656 when exposed to geraniol (0.25%, ... Time-kill analysis of a stationary phase culture of Burkholderia cenocepacia strain LMG 16656 when exposed to geraniol (0.25%, ... Burkholderia cepacia gas chromatography analysis MBC GC ...
Efflux pump genes of the resistance-nodulation-division family in Burkholderia cenocepacia genome. BMC Microbiol. 6:66. doi: ... Deciphering the role of RND efflux transporters in Burkholderia cenocepacia. PLoS One 6:e18902. doi:10.1371/journal.pone. ... Involvement of toxin-antitoxin modules in Burkholderia cenocepacia biofilm persistence. Pathog. Dis. 71:326-335. doi:10.1111/ ... Evaluation of the efficacy of disinfection procedures against Burkholderia cenocepacia biofilms. J. Hosp. Infect. 70:361-368. ...
  • B. cenocepacia harbours a large, multi-replicon genome with a high GC-content, the reference genome of strain J2315 includes 7374 annotated genes. (nih.gov)
  • RNA extracted from B. cenocepacia J2315 biofilms was analysed by differential RNA-sequencing and the resulting dataset compared to data derived from conventional, global RNA-sequencing. (nih.gov)
  • Mapping transcription start sites across the B. cenocepacia genome added relevant information to the J2315 annotation. (nih.gov)
  • In cystic fibrosis epidemic Burkholderia cenocepacia strain J2315, we identified a unique TAA (BCAM0224)-encoding gene, previously described as being implicated in virulence. (asm.org)
  • In the present study, the effects of deletion of the 16 operons encoding resistance-nodulation-cell division (RND)-type efflux pumps in B. cenocepacia strain J2315 were investigated by determining the MICs of various antibiotics and by investigating the antibiofilm effect of these antibiotics. (asm.org)
  • Our data indicate that the RND-3 and RND-4 efflux pumps are important for resistance to various antimicrobial drugs (including tobramycin and ciprofloxacin) in planktonic B. cenocepacia J2315 populations, while the RND-3, RND-8, and RND-9 efflux systems protect biofilm-grown cells against tobramycin. (asm.org)
  • Results from the RT-qPCR experiments on the wild-type strain B. cenocepacia J2315 suggest that there is little regulation at the level of mRNA expression for these efflux pumps under the conditions tested. (asm.org)
  • In the genome of B. cenocepacia strain J2315, a large number of efflux systems have been identified ( 7 - 10 ). (asm.org)
  • The RND-3 (BCAL1674 to BCAL1676) and RND-4 (BCAL2820 to BCAL2822) efflux systems were shown to contribute to the intrinsic resistance of B. cenocepacia J2315 to various compounds and to mediate accumulation of quorum-sensing molecules in the growth medium ( 8 ). (asm.org)
  • Genome analysis and homology searches revealed 14 open reading frames encoding putative drug efflux pumps belonging to RND family in B. cenocepacia J2315 strain. (biomedcentral.com)
  • High levels of AGS3 in macrophages enhanced their resistance to infection by Burkholderia cenocepacia J2315, Mycobacterium tuberculosis , and methicillin-resistant Staphylococcus aureus , whereas AGS3-deficient macrophages were more susceptible. (jimmunol.org)
  • Vacuoles containing B. cenocepacia strain J2315, an isolate of the transmissible ET12 clone, recruited Rab5 and synthesized phosphatidylinositol-3-phosphate, indicating progression to the early phagosomal stage. (qub.ac.uk)
  • Fang2011 - Genome-scale metabolic network of Burkholderia cenocepacia (iKF1028) This model is described in the article: Exploring the metabolic network of the epidemic pathogen Burkholderia cenocepacia J2315 via genome-scale reconstruction. (omicsdi.org)
  • Strain B. cenocepacia J2315 is the most infectious isolate from CF patients. (omicsdi.org)
  • There is a strong demand to reconstruct a genome-scale metabolic network of B. cenocepacia J2315 to systematically analyze its metabolic capabilities and its virulence traits, and to search for potential clinical therapy targets. (omicsdi.org)
  • RESULTS: We reconstructed the genome-scale metabolic network of B. cenocepacia J2315. (omicsdi.org)
  • The model iKF1028 captures important metabolic capabilities of B. cenocepacia J2315 with a particular focus on the biosyntheses of key metabolic virulence factors to assist in understanding the mechanism of disease infection and identifying potential drug targets. (omicsdi.org)
  • Based on the model, the genome annotation of B. cenocepacia J2315 was refined and 24 genes were properly re-annotated. (omicsdi.org)
  • CONCLUSIONS: As the first genome-scale metabolic network of B. cenocepacia J2315, iKF1028 allows a systematic study of the metabolic properties of B. cenocepacia and its key metabolic virulence factors affecting the CF community. (omicsdi.org)
  • 1] Transcription profiling of one Burkholderia cenocepacia clinical isolate, J2315, versus a soil isolate, HI2424, in conditions mimicking CF sputum [2] Transcription profiling of Burkholderia cenocepacia isolates J2315 and HI2424 in media mimicking CF sputum or the soil environment Overall design: [1] J2315 vs. HI2424 cells in the same condition. (omicsdi.org)
  • Burkholderia cenocepacia J2315 belongs to the ET12 lineage which has caused outbreaks among cystic fibrosis patients worldwide. (omicsdi.org)
  • DNA probes for IS commonly found in B. cenocepacia were designed using the B. cenocepacia J2315 genome. (plymouth.ac.uk)
  • Interestingly, when the closely related B. cenocepacia strain J2315 was exposed to oxidative stress, no movement of ISBcen20 was detected. (plymouth.ac.uk)
  • Burkholderia cenocepacia J2315 escapes to the cytosol and actively subverts autophagy in human macrophages. (microbiologyresearch.org)
  • 5: 83 Abstract: BACKGROUND: Burkholderia cenocepacia is a threatening nosocomial epidemic pathogen in patients with cystic fibrosis (CF) or a compromised immune system. (omicsdi.org)
  • All genomovars have been shown to cause infections, and B. cenocepacia and B. multivorans (genomovars III and II, respectively) are the genomovars most frequently isolated from CF patients ( 4 - 7 ). (cdc.gov)
  • The IST439 OAg biosynthetic gene cluster contains 7 of 23 genes that are closely homologous to genes found in B. multivorans , another member of the Burkholderia cepacia complex. (frontiersin.org)
  • B. cenocepacia and Burkholderia multivorans are currently the Bcc species most frequently isolated from clinical samples ( 1 , 4 ). (asm.org)
  • Although there are considerable regional differences, the majority of patients with cystic fibrosis worldwide are infected with either Burkholderia multivorans or Burkholderia cenocepacia ( 2 ). (asm.org)
  • B. cenocepacia , B. multivorans , B. stabilis , B. contaminans , and B. vietnamiensis [ 1 ]. (hindawi.com)
  • B. cenocepacia and Burkholderia multivorans account for the majority of infections in CF, and major epidemic clones have been recognized throughout the world. (cdc.gov)
  • Burkholderia cepacia genomovars I and VI, Burkholderia multivorans (genomovar II), Burkholderia cenocepacia (genomovar III), Burkholderia stabilis (genomovar IV), Burkholderia vietnamiensis (genomovar V), Burkholderia ambifaria (genomovar VII), Burkholderia anthina (genomovar VIII), and Burkholderia pyrrocinia (genomovar IX) ( 2 , 9 , 10 ). (asm.org)
  • B. cenocepacia (n=6), B. multivorans (n=3), B. stabilis (n=3). (ersjournals.com)
  • After 5 yrs, three B. cenocepacia - and one B. multivorans -colonised patients had died. (ersjournals.com)
  • B. multivorans (n=9), B. stabilis (n=1) and B. cenocepacia (n=2). (ersjournals.com)
  • Burkholderia multivorans in 1999. (ersjournals.com)
  • The most commonly isolated Bcc species in CF patients are B. multivorans, B. cenocepacia and B. stabilis . (ersjournals.com)
  • B. multivorans , B. cenocepacia and B. stabilis ) 10 . (ersjournals.com)
  • Burkholderia cenocepacia infection often leads to fatal cepacia syndrome in cystic fibrosis patients. (nih.gov)
  • We report a case of a vaginal infection caused by a strain of Burkholderia cenocepacia . (cdc.gov)
  • We report a case of vaginal infection, caused by B. cenocepacia, in a patient affected by smoldering myeloma, and chronic hepatitis C virus (HCV) infection. (cdc.gov)
  • Burkholderia cenocepacia is an opportunistic pathogen associated with chronic lung infections and increased risk of death in patients with cystic fibrosis (CF). In this work, we investigated the lipopolysaccharide (LPS) of clinical variants of B. cenocepacia that were collected from a CF patient over a period of 3.5 years, from the onset of infection until death by necrotizing pneumonia (cepacia syndrome). (frontiersin.org)
  • This study provides support to the notion that OAg LPS modifications are an important factor in the adaptation of B. cenocepacia to chronic infection and that the heterogeneity of OAgs relates to variation within the OAg gene cluster, indicating that the gene cluster might have been assembled through multiple horizontal transmission events. (frontiersin.org)
  • We previously made a link in Burkholderia cenocepacia between PA degradation and virulence by showing that insertional mutagenesis of paaA and paaE genes, that encode part of a multicomponent oxidase of PA-CoA, results in PA-conditional growth and an attenuated killing phenotype in the Caenorhabditis elegans model of infection. (frontiersin.org)
  • We used the C. elegans model of infection to screen a mini-Tn 5 mutant library of B. cenocepacia H111 for attenuated virulence. (asm.org)
  • In this work, we characterize later-stage infection of macrophages by B. cenocepacia , showing replication within an acidified endosomal compartment suggestive of a phagolysosome. (uwo.ca)
  • As a whole, secretion systems appear to increase pathogenicity in macrophages while limiting the spread of B. cenocepacia infection. (uwo.ca)
  • Loutet, SA & Valvano, M 2010, ' A Decade of Burkholderia cenocepacia Virulence Determinant Research ', Infection and Immunity , vol. 78, no. 10, pp. 4088-4100. (qub.ac.uk)
  • Members of the Burkholderia cepacia complex, normally present in soil microbiota, cause nosocomial lung infection especially in hospitalized cystic fibrosis patients. (microbiologyresearch.org)
  • Transcriptomic profiling across nine growth conditions was performed to identify the global gene expression changes made when B. cenocepacia changes niches from an environmental lifestyle to infection. (cf.ac.uk)
  • By creating isogenic PSCP mutants, we determined that PSCP is required for the maximal virulence of B. cenocepacia K56-2 in an experimental model of chronic lung infection. (ucalgary.ca)
  • IST439 was the first B. cenocepacia isolate recovered from the patient and it is thought to have initiated the infection while the IST4113 isolate was recovered three years later, after a period of exacerbated infection that compelled the patient to hospitalization and intravenous therapy with gentamicine and ceftazidime. (omicsdi.org)
  • SARS-CoV-2 and Burkholderia cenocepacia infection in a patient with Cystic Fibrosis: An unfavourable conjunction? (cdc.gov)
  • The effects of the concomitant infection by COVID-19 and Burkholderia cepacia (Bc) in CF are not known. (cdc.gov)
  • Clinafloxacin for Treatment of Burkholderia cenocepacia Infection in a" by Akshu Balwan, David P Nicolau et al. (mainehealth.org)
  • Clinafloxacin for Treatment of Burkholderia cenocepacia Infection in a Cystic Fibrosis Patient. (mainehealth.org)
  • Respiratory infection with Burkholderia cenocepacia is associated with accelerated decline in lung function and increased mortality in cystic fibrosis (CF) patients (A. M. Jones, M. E. Dodd, J. R. W. Govan, V. Barcus, C. J. Doherty, J. Morris, and A. K. Webb, Thorax 59:948-951, 2004, http://dx.doi.org/10.1136/thx.2003.017210). (mainehealth.org)
  • B. cenocepacia often possesses innate resistance to multiple antimicrobial classes, making eradication uncommon in established infection (P. B. Davis, Am J Respir Crit Care Med 173:475-482, 2006, http://dx.doi.org/10.1164/rccm.200505-840OE). (mainehealth.org)
  • We report the use of clinafloxacin in a CF patient with advanced B. cenocepacia infection, present pharmacokinetic (PK) data, and discuss the potential therapeutic role of clinafloxacin in patients with this condition. (mainehealth.org)
  • Here we constructed and characterized a B. cenocepacia tonB mutant strain, which was unable to actively transport iron, to test whether this single gene deletion mutant (strain renamed GAP001) protected against an acute respiratory B. cenocepacia lethal infection. (utmb.edu)
  • Chronic infection with opportunistic pathogens including Burkholderia cepacia complex (Bcc) is a hallmark of cystic fibrosis (CF). We investigated the adaptive mechanisms facilitating chronic lung infection in sequential Bcc isolates from two siblings with CF (P1 and P2), one of whom also experienced intermittent blood-stream infections (P2). (maynoothuniversity.ie)
  • Agodi A , Mahenthiralingam E , Bachitta M , Giannino V , Sciacca A , Stefani S . Burkholderia cepacia complex infection in Italian patients with cystic fibrosis: prevalence, epidemiology, and genomovar status. (cdc.gov)
  • Burkholderia cenocepacia infection: disruption of phagocyte immune functions through Rho GTPase inactivation. (qxmd.com)
  • Patients positive for human immunodeficiency virus (HIV) or Burkholderia cenocepacia infection. (clinicaltrials.gov)
  • Burkholderia cenocepacia has been a very hard to treat bacterial infection for our Cystic Fibrosis community. (cysticfibrosis.com)
  • Burkholderia cepacia infection is much less common, but it is notorious for being associated with cross infection and the possibility of rapid deterioration, known as the cepacia syndrome 2 - 4 . (ersjournals.com)
  • A rapid deterioration and patient-to-patient transmission is probably more likely with B. cenocepacia infection and colonisation 8 , 9 . (ersjournals.com)
  • Infections with B. cenocepacia are very difficult to treat due to their high intrinsic resistance to most antibiotics. (nih.gov)
  • In particular, the lack of efficacious antimicrobial treatments for infections caused by the Burkholderia cepaci a complex is causing high morbidity and mortality of cystic fibrosis patients and of patients with nosocomial infections. (asm.org)
  • Burkholderia cenocepacia is an opportunistic pathogen causing life-threatening infections in cystic fibrosis and other immunocompromised patients. (uwo.ca)
  • Burkholderia cenocepacia is notorious for causing respiratory tract infections in people with cystic fibrosis. (asm.org)
  • Species belonging to the Burkholderia cepacia complex (Bcc), a cluster of phylogenetically closely related and phenotypically similar Gram-negative bacteria, can cause severe respiratory tract infections in people with cystic fibrosis ( 1 ). (asm.org)
  • Infections with B. cenocepacia are particularly difficult to treat due to their high level of resistance against a wide range of antimicrobial agents ( 1 , 3 , 4 ). (asm.org)
  • The Burkholderia cepacia complex (Bcc) is a group of genetically related environmental bacteria that can cause chronic opportunistic infections in patients with cystic fibrosis (CF) and other underlying diseases. (qub.ac.uk)
  • A common theme emerging from these studies is that B. cenocepacia establishes chronic infections in immuno-compromised patients, which depend more on determinants mediating host niche adaptation than those involved directly in host cells and tissue damage. (qub.ac.uk)
  • Burkholderia cenocepacia is recognized as opportunistic pathogen that can cause lung infections in cystic fibrosis patients. (biomedcentral.com)
  • A hallmark of B. cenocepacia infections is the inability to eradicate the organism because of multiple intrinsic antibiotic resistance. (biomedcentral.com)
  • B. cenocepacia is known for its ability to cause lung infections in people with cystic fibrosis and it possesses a large 8 Mb multireplicon genome encoding a wide array of pathogenicity and fitness genes. (cf.ac.uk)
  • These infections are caused, among others, by opportunistic pathogens, for example Burkholderia cenocepacia(B. cenocepacia). (knowledgeforgrowth.be)
  • Pseudomonas aeruginosa and Burkholderia cenocepacia are the causative agents of chronic airway infections and a major cause of mortality in cystic fibrosis patients. (ucalgary.ca)
  • Despite advances in anti-microbial therapy, the treatment of infections caused by P. aeruginosa and B. cenocepacia remains a challenge. (ucalgary.ca)
  • These studies provide the initial steps toward producing anti-peptide 15 immunotherapeutic agents for use in P. aeruginosa or B. cenocepacia respiratory infections. (ucalgary.ca)
  • A glycoengineered antigen exploiting a conserved protein O-glycosylation pathway in the Burkholderia genus for detection of glanders infections. (pubfacts.com)
  • Burkholderia cenocepacia can cause serious infections and epidemics in patients with cystic fibrosis (CF). A CF population in the Czech Republic experienced an epidemic outbreak caused by a B. cenocepacia ST-32 strain. (plymouth.ac.uk)
  • Burkholderia cenocepacia is an opportunistic pathogen prevalent in cystic fibrosis patients, which is particularly difficult to treat, causing chronic and eventually fatal infections. (utmb.edu)
  • Vaccines, and live attenuated vaccines, are an unexplored avenue to treat B. cenocepacia infections. (utmb.edu)
  • The Burkholderia cepacia complex ( Bcc ) bacteria are opportunistic pathogens which cause nosocomial infections and are especially dangerous for cystic fibrosis (CF) patients. (hindawi.com)
  • In the early 1980s Burkholderia cepaciabecame associated with epidemics of severe lung infections ('cepacia syndrome') traced, in many cases, to CF treatment centers and social gatherings where CF patients apparently acquired the disease from other B. cepacia-infected individuals (19). (apsnet.org)
  • In this environment, we studied adaptation by a soil isolate of Burkholderia cenocepacia , an opportunistic pathogen responsible for fatal biofilm-associated lung infections in CF patients ( 14 , 15 ). (pnas.org)
  • Pseudomonas and Burkholderia bacteria cause devastating infections in people with cystic fibrosis and we have used molecular biology and genomic approaches to track their ability to spread between patients, resist killing by antibiotics and cause lung disease. (cardiff.ac.uk)
  • For example, our recent discovery that Burkholderia bacteria can produce novel polyketide antibiotics that target multidrug resistant bacterial infections is very exciting. (cardiff.ac.uk)
  • This is where I began to developing expertise in the cystic fibrosis microbiology, working on Pseudomonas aeruginosa and Burkholderia cepacia complex bacteria that cause devastating lung infections in these individuals. (cardiff.ac.uk)
  • CC Burkholderia cenocepacia HI2424 plasmid 1, complete sequence. (univ-lyon1.fr)
  • Four thousand ten transcription start sites were mapped over the whole B. cenocepacia genome and the primary transcription start site of 2089 genes expressed in B. cenocepacia biofilms were defined. (nih.gov)
  • Contributing to this is the fact that Bcc strains, including B. cenocepacia strains, readily form biofilms on various biotic and abiotic surfaces ( 5 ). (asm.org)
  • The consensus phylogenetic tree of recA DNA sequences of Burkholderia cepacia complex strains, representative of each genomovar, and of the B. cenocepacia isolate (GenBank accession no. (cdc.gov)
  • Several transmissible Burkholderia cenocepacia strains that infect multiple cystic fibrosis patients contain a genomic island designated as the c eno c epacia i sland (cci). (asm.org)
  • B. cenocepacia strains also have a cepIR quorum-sensing system. (asm.org)
  • The majority of transmissible or epidemic Bcc strains are B. cenocepacia ( 26 , 31 , 48 , 59 ), and epidemiological studies have shown this species is highly virulent, causing significant mortality among CF patients ( 28 , 30 ). (asm.org)
  • The two quorum sensing systems exert reciprocal regulation of many genes likely enabling fine-tuned control of quorum sensing gene expression in B. cenocepacia strains carrying the cenocepacia island. (biomedcentral.com)
  • Distribution of cepacian biosynthesis genes among environmental and clinical Burkholderia strains and role of cepacian exopolysaccharide in resistance to stress conditions. (burkholderia.com)
  • Strains of the Burkholderia cepacia complex can survive within macrophages by arresting the maturation of phagocytic vacuoles. (qub.ac.uk)
  • Strains belonging to the ET12 lineage are characterized by the presence of the "Burkholderia cepacia epidemic strain marker" (BCESM). (omicsdi.org)
  • Individual normalized intensity of Burkholderia cenocepacia and Burkholderia seminalis hybridized in a B. cenocepacia array Overall design: A collection of 199 Burkholderia isolates were hybridized against an array targeting five B. cenocepacia strains in order to understand the gene distribution within this species. (omicsdi.org)
  • Different species Burkholderia and Achromobacter strains all demonstrated similar BiofilmReg operon structure. (hindawi.com)
  • Only recA DNA sequences of reference B. cenocepacia strains (genomovar III, lineage IIIB) are included in the tree ( 4 , 13 ). (cdc.gov)
  • We report here that deletion of BCAM0628, encoding a predicted low molecular weight protein tyrosine phosphatase (LMW-PTP) that is restricted to B. cenocepacia strains of the transmissible ET-12 clone, accelerates the maturation of the BcCVs. (microbiologyresearch.org)
  • Over the past 20 years a group of closely related Burkholderia species termed the B. cepacia complex (Bcc) have emerged as opportunistic pathogens of particular importance in people with cystic fibrosis (CF) and chronic granulomatous disease ( 31 , 33 ). (asm.org)
  • The Burkholderia cepacia complex is a group of gram-negative bacteria comprising at least nine species ( 33 , 48 ) which have emerged as multidrug-resistant nosocomial pathogens in immunocompromised patients, particularly in those with chronic granulomatous diseases and cystic fibrosis. (asm.org)
  • Burkholderia cenocepacia belongs to a group of closely related organisms called the B. cepacia complex (Bcc) which are important opportunistic human pathogens. (biomedcentral.com)
  • Burkholderia cenocepacia is a Gram-negative aerobic bacterium that belongs to a group of opportunistic pathogens displaying diverse environmental and pathogenic lifestyles. (cf.ac.uk)
  • Here, we will discuss six zebrafish models for important human pathogens ( Mycobacterium , Salmonella , Burkholderia , Staphylococcus , Shigella and Candida ), emphasizing the novel insights that these models have recently provided into macrophage biology and highlighting how this could lead to the finding of new host-derived therapeutic strategies. (biologists.org)
  • As B. cenocepacia and C. albicans are frequently encountered human pathogens, identification of the BDSF signal and its activity thus provides a new insight into the molecular grounds of their antagonistic interactions whose importance to microbial ecology and pathogenesis is now becoming evident. (pubfacts.com)
  • Organisms of the Burkholderia cepacia complex are especially important pathogens in cystic fibrosis (CF), with a propensity for patient-to-patient spread and long-term respiratory colonization. (cdc.gov)
  • The Burkholderia cepacia complex (Bcc) is a group of at least 9 closely related species (often called "genomovars") that is currently attracting considerable attention for their extraordinary versatility as plant pathogens, saprophytes, biocontrol agents, bioremediation agents, and human pathogens. (apsnet.org)
  • My group has studied the pathogenesis and ecology of bacterial opportunistic pathogens, ranging from Pseudomonas aeruginosa to mycobacteria, with a major focus on Burkholderia cepacia complex bacteria. (cardiff.ac.uk)
  • In the last few years, we made a very exciting discovery that the Burkholderia bacteria I had studied as pathogens, also make some very potent antibiotics which kill other multidrug resistant bacteria and fungi. (cardiff.ac.uk)
  • A = averages) shows the log 2 fold changes in transcript expression in B. cenocepacia H111 grown under nitrogen-limited (S) versus nitrogen-replete (N) conditions. (asm.org)
  • Box plot for the numbers of reads per kilobase per million (RPKM) for genes in the wild-type strain B. cenocepacia H111, the σ 54 mutant (mt), and the complemented mutant (comp). (asm.org)
  • Cells of the wild-type strain B. cenocepacia H111 (B and F), the σ 54 mutant (C and G), and the complemented mutant (D and H) were grown on plates containing limiting amounts of ammonium. (asm.org)
  • C) Swimming motility of the wild-type strain B. cenocepacia H111, the σ 54 mutant, and the complemented mutant. (asm.org)
  • Virulence of B. cenocepacia H111 against C. elegans is dependent on σ 54 . (asm.org)
  • Using a discovery shotgun proteomics approach, the expressed proteome of Burkholderia cenocepacia (strain H111) was analyzed under normal and starved nitrogen growth conditions. (omicsdi.org)
  • The opportunistic human pathogen Burkholderia cenocepacia H111 uses two chemically distinct signal molecules for controlling gene expression in a cell density-dependent manner: N-acyl-homoserine lactones (AHLs) and cis-2-dodecenoic acid (BDSF). (unibas.ch)
  • In this study we analysed the transcriptional profile of B. cenocepacia H111 upon artificially altering the cellular c-di-GMP level. (unibas.ch)
  • In dieser Arbeit wurde der Nematode Caenorhabditis elegans als Modell zur Untersuchung der Pathogenität des klinischen Isolats B. cenocepacia H111 verwendet. (tum.de)
  • Durch den Einsatz spezifischer QS-Hemmstoffe konnte die Pathogenität von B. cenocepacia H111 und dem bei Patienten mit Mukoviszidose vorherrschenden Stamm, Pseudomonas aeruginosa, erheblich vermindert werden. (tum.de)
  • Burkholderia cenocepacia is a species of Gram-negative bacteria that is common in the environment, can form a biofilm with itself, is resistant to many antibiotics and may cause disease in plants. (wikipedia.org)
  • See also: Burkholderia thailandensis sRNA Originally defined as B. cepacia, the group has now been split into nine species, and B. cenocepacia is one of the most intensively-studied. (wikipedia.org)
  • To date, the genus Burkholderia comprises more than 30 species, including the Burkholderia cepacia complex, B. mallei , and B. pseudomallei ( 2 ). (cdc.gov)
  • Whole-Genome Assemblies of 56 Burkholderia Species. (kegg.jp)
  • B. cenocepacia is the most commonly reported Bcc species isolated from patients with CF ( 26 , 48 ). (asm.org)
  • The Burkholderia cepacia complex (Bcc) consists of 17 closely related, but phenotypically diverse, bacterial species. (asm.org)
  • Burkholderia cenocepacia has been the focus of attention because initially it was the most common Bcc species isolated from patients with CF in North America and Europe. (qub.ac.uk)
  • Transcriptional profiling of RNA-seq data from two Burkholderia species grown under conditions mimicking the cystic fibrosis lung and the soil environment Overall design: Two condition, two strain experiment. (omicsdi.org)
  • Pseudomonas aeruginosa and species of the Burkholderia cepacia complex (Bcc) can co-exist in the lower airways, however several studies have shown that P. aeruginosa can effectively kill the Bcc in vitro, for which hydrogen cyanide (HCN) was recently proposed to play a critical role. (dartmouth.edu)
  • Here we show that modification of the environment (i.e., culture medium), long-term genetic adaptation of P aeruginosa to the cystic fibrosis (CF) lung, or the addition of another bacterial species to the community can alter the sensitivity of Burkholderia cenocepacia to P aeruginosa toxins. (dartmouth.edu)
  • Among these, B. cenocepacia was more abundant, and the Russian epidemic strain ST (sequence type) 709 belonged to this species. (hindawi.com)
  • Coenye T , Vandamme P . Diversity and significance of Burkholderia species occupying diverse ecological niches. (cdc.gov)
  • Molecular epidemiology of Burkholderia species. (cdc.gov)
  • Spilker T, Gill GH, Campbell PW, Liu L, Mahenthiralingam E. Disproportionate distribuition of Burkholderia cepacia complex species and transmissibility markers in cystic fibrosis. (cdc.gov)
  • Vermis K , Coenye T , Mahenthiralingam E , Nelis HJ , Vandamme P . Evaluation of species-specific recA -based PCR tests for genomovar level identification within the Burkholderia cepacia complex. (cdc.gov)
  • Compared to the parental strain and deletion mutants in other LMW-PTPs that are widely conserved in Burkholderia species, a greater proportion of BcCVs containing the Δ BCAM0628 mutant were targeted to the lysosome. (microbiologyresearch.org)
  • Burkholderia cepacia is the type species of the genus Burkholderia and is a ubiquitous multidrug-resistant, motile, non-glucose-fermenting, gram-negative organism found in water and soil ( 1 ). (cdc.gov)
  • Typically, each species of Burkholderia has 3-5 homologous tsRNAs. (wikipedia.org)
  • Experiments with four species of the Burkholderia lineage showed conserved and constitutive expression of tsRNAs in logarithmic growth phases. (wikipedia.org)
  • Although this species has been identified as the most prevalent and pathogenic of the Burkholderia cepacia complex species in CF patients ( 14 ), relatively little is known about how it adapts to a biofilm lifestyle or to the CF lung environment ( 5 , 16 ). (pnas.org)
  • DNA-DNA hybridisation experiments between isolates representing Burkholderia cepacia genomovar III recA lineages IIIA and IIIB reinforced the classification of both phylogenetic subgroups as a single genospecies, distinct from B. cepacia (genomovar I). A formal classification of B. cepacia genomovar III encompassing the recA lineages IIIA and IIIB, and the new recA lineages IIIC and IIID, as B. cenocepacia sp. (nih.gov)
  • Petrucca A , Cipriani P , Valenti P , Santapaola D , Cimmino C , Scoarughi GL , Molecular characterization of Burkholderia cepacia isolates from cystic fibrosis (CF) patients in an Italian CF center. (cdc.gov)
  • These mutations in turn were surprisingly well represented among mutations that evolved in cystic fibrosis isolates of both Burkholderia and Pseudomonas . (pnas.org)
  • AY278465 Burkholderia cenocepacia strain LMG 16656 outer membrane lipoprotein precursor (opcL) gene, partial cds. (atcc.org)
  • AF148556 Burkholderia cepacia genomovar III 16S ribosomal RNA gene, partial sequence. (atcc.org)
  • An antibiotic efflux gene cluster that confers resistance to chloramphenicol, trimethoprim, and ciprofloxacin has been identified in Burkholderia cenocepacia (genomovar III), an important cystic fibrosis pathogen. (jci.org)
  • Using reporter activity assays, we demonstrate that 3-OHPA activated PA degradation gene promoters of Burkholderia cenocepacia K56-2 in a paaK1 -dependent manner, while 4-OHPA had no effect. (frontiersin.org)
  • We hypothesize that B. cenocepacia alters gene expression after internalization by macrophages, inducing genes involved in intracellular survival and host adaptation. (uwo.ca)
  • We examine differential gene expression by intracellular B. cenocepacia using selective capture of transcribed sequences (SCOTS) with both competitive enrichment and microarray analysis. (uwo.ca)
  • B. cenocepacia utilizes a mechanism of cell-cell communication called quorum sensing to control gene expression including genes involved in virulence. (biomedcentral.com)
  • Global gene expression profiles during growth in stationary phase were generated using microarrays of B. cenocepacia cepR , cciR and cepRcciIR mutants. (biomedcentral.com)
  • For the first time, the influence of CciIR on global gene regulation in B. cenocepacia has been elucidated. (biomedcentral.com)
  • The 14 RND pumps gene we have identified in the genome of B. cenocepacia suggest that active efflux could be a major mechanism underlying antimicrobial resistance in this microorganism. (biomedcentral.com)
  • In this study, we cloned and sequenced the zmpA gene encoding for PSCP, an extracellular metalloprotease of B. cenocepacia. (ucalgary.ca)
  • The gene is highly conserved and widespread in the Burkholderia cepacia complex. (pubfacts.com)
  • Dabei wurde die Bedeutung der QS-Gene cepR und rsuA für die Pathogenität von B. cenocepacia bestätigt. (tum.de)
  • Genes and novel regulatory RNAs putatively involved in B. cenocepacia biofilm formation were identified. (nih.gov)
  • In this study, we utilized TraDIS (Transposon Directed Insertion-site Sequencing) as a genome-wide screening tool to facilitate the identification of B. cenocepacia genes essential for its growth and viability. (nih.gov)
  • The saturated library allowed for the identification of 383 genes that were predicted to be essential in B. cenocepacia. (nih.gov)
  • We extended the application of TraDIS to identify conditionally essential genes required for in vitro growth and revealed an additional repertoire of 439 genes to be crucial for B. cenocepacia growth under nutrient-depleted conditions. (nih.gov)
  • The library of B. cenocepacia mutants can subsequently be subjected to various biologically related conditions to facilitate the discovery of genes involved in niche adaptation as well as pathogenicity and virulence. (nih.gov)
  • Here, we present a method based on delivery of the tightly regulated rhamnose-inducible promoter P rhaB for identifying essential genes and operons in Burkholderia cenocepacia . (asm.org)
  • This system allows us to identify essential genes and operons in B. cenocepacia and provides a new tool for systematically identifying and functionally characterizing essential genes at the genomic level. (asm.org)
  • Deletion of various SCOTS-identified genes affects B. cenocepacia entry into macrophages and intracellular replication, as well as host-directed cytotoxicity and spread to neighbouring cells. (uwo.ca)
  • Expression of secretion system genes is differentially-regulated by intracellular B. cenocepacia . (uwo.ca)
  • Further investigation into the remaining SCOTS-identified genes, as well as putative secreted effectors, will provide a better understanding of the adaptive responses of intracellular B. cenocepacia , leading to life in a phagosomal niche and host cell cytotoxicity. (uwo.ca)
  • Novel genes under the control of the CepIR and CciIR quorum sensing systems in B. cenocepacia have been identified. (biomedcentral.com)
  • As Resistance-Nodulation-Division (RND) efflux systems are responsible for much of the intrinsic multidrug resistance in Gram-negative bacteria, this study aims to identify RND genes in the B. cenocepacia genome and start to investigate their involvement into antimicrobial resistance. (biomedcentral.com)
  • Previous work demonstrated that B. cenocepacia phenylacetic acid (PA) catabolic genes are up-regulated during growth in SCFM and are required for full virulence in a Caenorhabditis elegans host model. (umanitoba.ca)
  • Time-kill analysis of a stationary phase culture of Burkholderia cenocepacia strain LMG 16656 when exposed to geraniol (0.25%, v/v) and 5 mM EDTA. (figshare.com)
  • Delayed association of the NADPH oxidase complex with macrophage vacuoles containing the opportunistic pathogen Burkholderia cenocepacia. (qxmd.com)
  • Here, we characterized this multifunctional protein, trying to establish its role in B. cenocepacia pathogenicity. (asm.org)
  • The book was edited by Tom Coenye and myself and serves as an excellent update to the highly successful first book: "Burkholderia: molecular microbiology and genomics" ( http://www.horizonpress.com/backlist/horizonbioscience/burk.html ). (cardiff.ac.uk)
  • While Burkholderia bacteria and cystic fibrosis microbiology remain major research foci, my interests are still wide and I am always getting involved in lots of very interesting molecular microbiology projects. (cardiff.ac.uk)
  • Phenotypic methods for determining genomovar status of the Burkholderia cepacia complex. (cdc.gov)
  • Burkholderia cenocepacia is a member of the B. cepacia complex (Bcc), a group of opportunistic bacteria that infect the airways of patients with cystic fibrosis (CF) and are extraordinarily resistant to almost all clinically useful antibiotics. (microbiologyresearch.org)
  • nov. and Burkholderia pyrrocinia , two additional Burkholderia cepacia complex bacteria, may confound results of new molecular diagnostic tools. (cdc.gov)
  • Additionally, they are not toxic to the bacteria in which these RNA molecules are found (B. cenocepacia). (wikipedia.org)
  • We are pleased to announced the forthcoming publication of a second book on Burkholderia bacteria: "Burkholderia: from genomes to function" which can be previewed at Horizon Press ( http://www.horizonpress.com/burk2 ). (cardiff.ac.uk)
  • The characterization of antibiotic efflux pumps in B. cenocepacia is an obligatory step prior to the design of specific, potent bacterial inhibitors for the improved control of infectious diseases. (biomedcentral.com)
  • We report here the isolation and characterization of a novel signaling molecule, cis-2-dodecenoic acid (BDSF), from Burkholderia cenocepacia. (pubfacts.com)
  • Characterization of the secondary structures of 4 tsRNAs in Burkholderia cenocepacia suggests they form double hairpin structures. (wikipedia.org)
  • Characterization of rhamnolipid production by Burkholderia glumae. (springer.com)
  • The OAg repeating unit of the LPS from IST439, the initial isolate, is a [→2)-β-D-Rib f -(1→4)-α-D-Gal p NAc-(1→] disaccharide, which was not previously described in B. cenocepacia . (frontiersin.org)
  • Burkholderia cenocepacia is an environmental organism and an unusual isolate from blood, although is a recognised cause of nosocomial outbreaks due to contamination of various fluids. (acipcconference.com.au)
  • In addition, the strong environmental protection response of B. cenocepacia is attributed to the biofilm formed by groups of the organism. (wikipedia.org)
  • Whether Burkholderia cepacia complex should be an objectionable organism in antiseptic solutions with acceptable total bacterial counts is controversial. (cdc.gov)
  • Complete Genome Sequence of Burkholderia cenocepacia Phage Magia. (pubfacts.com)
  • Complete Genome Sequence of Burkholderia cenocepacia Phage Mica. (pubfacts.com)
  • A novel sensor kinase-response regulator hybrid controls biofilm formation and type VI secretion system activity in Burkholderia cenocepacia. (burkholderia.com)
  • Here, we show that intracellular B. cenocepacia expressing a type VI secretion system (T6SS) affects the activation of the Rac1 and Cdc42 RhoGTPase by reducing the cellular pool of GTP-bound Rac1 and Cdc42. (qxmd.com)
  • Burkholderia cenocepacia type VI secretion system mediates escape of type II secreted proteins into the cytoplasm of infected macrophages. (qxmd.com)
  • Quantification of type VI secretion system activity in macrophages infected with Burkholderia cenocepacia. (qxmd.com)
  • Extracellular metalloproteases of pseudomonas aeruginosa and burkholderia cenocepacia as potential immunotherapeutic targets (Unpublished doctoral thesis). (ucalgary.ca)
  • Formerly known as Pseudomonas cepacia, the bacterium now known as Burkholderia cepacia, was first described in 1950 as the cause of sour skin of onions by Cornell University plant pathologist Walter Burkholder (1). (apsnet.org)
  • The bacterium survives within macrophages by interfering with typical endocytic trafficking, resulting in delayed maturation of a B. cenocepacia -containing phagosome. (uwo.ca)
  • Together, these studies advance our understanding of the intracellular behaviour of B. cenocepacia in macrophages. (uwo.ca)
  • B. cenocepacia survives inside macrophages, and this survival depends on the secretion of virulence factors, among which two (metallo)proteases. (knowledgeforgrowth.be)
  • We have previously shown that B. cenocepacia can survive in macrophages within membrane vacuoles [ B. cenocepacia -containing vacuoles (BcCVs)] that preclude fusion with the lysosome. (microbiologyresearch.org)
  • Depletion of the ubiquitin-binding adaptor molecule SQSTM1/p62 from macrophages harboring cftr ΔF508 mutation improves the delivery of Burkholderia cenocepacia to the autophagic machinery. (microbiologyresearch.org)
  • Therefore, T6SS-mediated deregulation of Rho family GTPases is a common mechanism linking disruption of the actin cytoskeleton and delayed NADPH oxidase activation in macrophages infected with B. cenocepacia. (qxmd.com)
  • Its overexpression in E. coli conferred resistance to several antibiotics and to ethidium bromide but it remains to be determined if this pump play a significant role in the antimicrobial intrinsic resistance of B. cenocepacia . (biomedcentral.com)
  • Nzula S , Vandamme P , Govan JR . Influence of taxonomic status on the in vitro antimicrobial susceptibility of the Burkholderia cepacia complex. (cdc.gov)
  • Oxidative stress of Burkholderia cenocepacia induces insertion sequence-mediated genomic rearrangements that interfere with macrorestriction-based genotyping. (plymouth.ac.uk)
  • Burkholderia cenocepacia induces neutrophil necrosis in chronic granulomatous disease. (medscape.com)
  • Here, we used RNA-seq to comprehensively and quantitatively assess the transcriptional response of the bacterial opportunistic cystic fibrosis (CF) pathogen and endemic soil dweller, Burkholderia cenocepacia, in conditions mimicking these 2 environments. (omicsdi.org)
  • Further analyses showed that Candida albicans germ tube formation was strongly inhibited by either coculture with B. cenocepacia or by exogenous addition of physiological relevant levels of BDSF, whereas deletion of Bcam0581 abrogated the inhibitory ability of the bacterial pathogen. (pubfacts.com)
  • The bacterial factors involved in B. cenocepacia intracellular survival are not fully elucidated. (microbiologyresearch.org)
  • Synthetic cystic fibrosis sputum medium (SCFM) is rich in amino acids and supports robust growth of Burkholderia cenocepacia, a member of the Burkholderia cepacia complex (Bcc). (umanitoba.ca)
  • Results: Efficient recombinant protein production and crystallization protocols were developed, and high-resolution crystal structures of IspF from P. falciparum ( Pf IspF) and B. cenocepacia ( Bc IspF) in complex with cytidine nucleotides determined. (edu.pl)
  • Draft Genome of Burkholderia cenocepacia TAtl-371, a Strain from the Burkholderia cepacia Complex Retains Antagonism in Different Carbon and Nitrogen Sources. (bvsalud.org)
  • Speert DP , Henry DA , Vandamme P , Corey M , Mahenthiralingam E . Epidemiology of Burkholderia cepacia complex in patients with cystic fibrosis, Canada. (cdc.gov)
  • Taxonomy and identification of the Burkholderia cepacia complex. (cdc.gov)
  • The impact of Burkholderia cepacia complex on morbidity in the Belgian cystic fibrosis population is high and not limited to Burkholderia cenocepacia . (ersjournals.com)
  • 5ixh is a 2 chain structure with sequence from Burkholderia cenocepacia bc7 . (proteopedia.org)
  • RT "Complete sequence of chromosome 2 of Burkholderia cenocepacia MC0- RT 3. (univ-lyon1.fr)
  • These results suggest that salicylate is a natural substrate for the efflux pump in B. cenocepacia and imply that the environment of low iron concentration in the cystic fibrosis lung can induce efflux-mediated resistance, even in the absence of antibiotic selective pressure. (jci.org)
  • Focusing on a pathogen of the cystic fibrosis lung, Burkholderia cenocepacia, we sequenced clones and metagenomes to unravel the mutations and evolutionary forces responsible for adaptation and diversification of a single biofilm community during 1,050 generations of selection. (pnas.org)
  • In this work, we aimed to determine the relevance of PaaK1 activity to the killing ability of B. cenocepacia to C. elegans . (frontiersin.org)
  • We also demonstrate that the intracellular c-di-GMP level determines the virulence of B. cenocepacia to Caenorhabditis elegans and Galleria mellonella. (unibas.ch)
  • Um Virulenzfaktoren zu identifizieren, die nicht durch das QS-System reguliert sind, wurde die Pathogenität von ca. 1.000 B. cenocepacia Tn5-Insertionsmutanten im C. elegans Modell untersucht. (tum.de)
  • Insgesamt wurde gezeigt, dass einzelne Virulenzfaktoren von B. cenocepacia in C. elegans keine (Protease- und Lipase-Aktivität sowie EPS-Produktion) oder nur eine geringe Rolle (Siderophore) spielen und dass die Pathogenität dieses Bakteriums durch ein komplexes Zusammenwirken verschiedener Faktoren und Mechanismen reguliert ist, die erst zum Teil aufgeklärt sind. (tum.de)
  • B. cenocepacia is intrinsically resistant to antibiotic therapy and often impossible to eradicate from lungs of infected CF patients [ 9 ]. (biomedcentral.com)
  • A transcriptomic analysis of RND-4 and RND-9 (BCAM1945 to BCAM1947) deletion mutants revealed that in B. cenocepacia , RND pumps also influence other phenotypic traits important for pathogenesis ( 9 ). (asm.org)
  • Deletion of the lxa locus resulted in B. cenocepacia mutants with aerobic growth deficiencies in minimal medium and compromised viability after prolonged incubation in the absence of oxygen. (cf.ac.uk)
  • Here, we present a genome-wide view of the molecular mutation spectrum in Burkholderia cenocepacia , a clinically relevant pathogen with high %GC content and multiple chromosomes. (genetics.org)
  • We find that B. cenocepacia has low genome-wide mutation rates with insertion-deletion mutations biased toward deletions, consistent with the idea that deletion pressure reduces prokaryotic genome sizes. (genetics.org)
  • Unlike prior studies of other organisms, mutations in B. cenocepacia are not AT biased, which suggests that at least some genomes with high %GC content experience unusual base-substitution mutation pressure. (genetics.org)
  • We specifically demonstrate that undefined rich media leads to higher susceptibility of B. cenocepacia to P. aeruginosa toxins like cyanide as compared to a synthetic medium (SCFM), that mimics the CF lung nutritional content. (dartmouth.edu)
  • In addition, we reported on the identification of lifestyle-specific efflux pumps involved in B. cenocepacia tolerance to the disinfectant chlorhexidine (CHX) ( 12 ). (asm.org)
  • We sequenced the draft genome of Burkholderia cenocepacia KC-01, isolated from the coastal saline soil, and identified several potential sRNAs in silico . (microbiologyresearch.org)
  • Here, we report and describe the improved, high- quality permanent draft genome of B. cenocepacia TAtl-371, which was sequenced using a combination of PacBio RS and PacBio RS II sequencing methods . (bvsalud.org)
  • The distribution of internal transcription start sites can be used to identify genomic islands in B. cenocepacia. (nih.gov)
  • In summary, transcriptomic profiling of B. cenocepacia revealed an unexpected ability of aerobic Burkholderia to persist in the absence of oxygen and identified the novel lxa locus as key determinant of this important ecophysiological trait. (cf.ac.uk)
  • Attachment was dramatically reduced in a B. cenocepacia K56-2Δlxa-locus deletion mutant, further indicating that it encodes protein(s) involved in host-cell attachment. (maynoothuniversity.ie)
  • Here we present crystal structures of Burkholderia cenocepacia haem importer BhuUV complexed with the periplasmic haem-binding protein BhuT and in the absence of BhuT. (rcsb.org)
  • Furthermore, proteomic analysis utilizing iTRAQ were used to study the protein expression of B. cenocepacia K56-2 grown in the amino acid-rich SCFM. (umanitoba.ca)
  • Interestingly, our results also showed the over-expression of flagellin and membrane efflux protein which are involved in the virulence of B. cenocepacia. (umanitoba.ca)
  • Jointly, these experiments indicate that B. cenocepacia express virulence factors capable of interfering with Rab7 function and thereby with membrane traffic. (qub.ac.uk)
  • Multidrug resistance in B. cenocepacia can be ascribed to different mechanisms, including the activity of efflux pumps and biofilm formation. (asm.org)
  • Recently, we showed that the RND-4 efflux system is involved in the resistance to a new thiopyridine drug effective against B. cenocepacia ( 11 ). (asm.org)
  • B. cenocepacia is an opportunistic human pathogen that is particularly problematic for patients suffering from cystic fibrosis. (omicsdi.org)
  • B. cenocepacia bacteraemia in two patients in the Canberra Hospital ICU prompted an investigation into a common source. (acipcconference.com.au)
  • Burkholderia cenocepacia infects patients with cystic fibrosis. (microbiologyresearch.org)
  • Burkholderia cenocepacia is a Gram-negative opportunistic pathogen of patients with cystic fibrosis and chronic granulomatous disease. (qxmd.com)
  • Similalrly, it was reported that B. cenocepacia strain PHDC recovered from most CF patients in the mid-Atlantic region of the USA could also be isolated from agricultural soils in the same region (32). (apsnet.org)