Burkholderia: A genus of gram-negative, aerobic, rod-shaped bacteria. Organisms in this genus had originally been classified as members of the PSEUDOMONAS genus but overwhelming biochemical and chemical findings indicated the need to separate them from other Pseudomonas species, and hence, this new genus was created.Burkholderia pseudomallei: A species of gram-negative, aerobic bacteria that causes MELIOIDOSIS. It has been isolated from soil and water in tropical regions, particularly Southeast Asia.Burkholderia Infections: Infections with bacteria of the genus BURKHOLDERIA.Burkholderia cepacia: A species of BURKHOLDERIA considered to be an opportunistic human pathogen. It has been associated with various types of infections of nosocomial origin.Burkholderia cepacia complex: A group of phenotypically similar but genotypically distinct species (genomovars) in the genus BURKHOLDERIA. They are found in water, soil, and the rhizosphere of crop plants. They can act as opportunistic human pathogens and as plant growth promoting and biocontrol agents.Burkholderia cenocepacia: A species of gram-negative bacteria that causes disease in plants. It is found commonly in the environment and is an opportunistic pathogen in humans.Melioidosis: A disease of humans and animals that resembles GLANDERS. It is caused by BURKHOLDERIA PSEUDOMALLEI and may range from a dormant infection to a condition that causes multiple abscesses, pneumonia, and bacteremia.Burkholderia gladioli: A species of gram-negative, aerobic bacteria that acts as both a human and plant pathogen.Glanders: A contagious disease of horses that can be transmitted to humans. It is caused by BURKHOLDERIA MALLEI and characterized by ulceration of the respiratory mucosa and an eruption of nodules on the skin.Mimosa: A plant genus of the family FABACEAE that contains kukulkanin, a CHALCONE.Cystic Fibrosis: An autosomal recessive genetic disease of the EXOCRINE GLANDS. It is caused by mutations in the gene encoding the CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR expressed in several organs including the LUNG, the PANCREAS, the BILIARY SYSTEM, and the SWEAT GLANDS. Cystic fibrosis is characterized by epithelial secretory dysfunction associated with ductal obstruction resulting in AIRWAY OBSTRUCTION; chronic RESPIRATORY INFECTIONS; PANCREATIC INSUFFICIENCY; maldigestion; salt depletion; and HEAT PROSTRATION.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Soil Microbiology: The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.RNA, Ribosomal, 16S: Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.Quorum Sensing: A phenomenon where microorganisms communicate and coordinate their behavior by the accumulation of signaling molecules. A reaction occurs when a substance accumulates to a sufficient concentration. This is most commonly seen in bacteria.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Bacterial Proteins: Proteins found in any species of bacterium.Bacterial Typing Techniques: Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.DNA, Ribosomal: DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.Acyl-Butyrolactones: Cyclic esters of acylated BUTYRIC ACID containing four carbons in the ring.Heteroptera: A suborder of HEMIPTERA, called true bugs, characterized by the possession of two pairs of wings. It includes the medically important families CIMICIDAE and REDUVIIDAE. (From Dorland, 28th ed)Environmental Microbiology: The study of microorganisms living in a variety of environments (air, soil, water, etc.) and their pathogenic relationship to other organisms including man.Biodegradation, Environmental: Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.Fenitrothion: An organothiophosphate cholinesterase inhibitor that is used as an insecticide.Virulence: The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.Eleocharis: A plant genus of the family CYPERACEAE.Genome, Bacterial: The genetic complement of a BACTERIA as represented in its DNA.Genes, Bacterial: The functional hereditary units of BACTERIA.Gene Expression Regulation, Bacterial: Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.Onions: Herbaceous biennial plants and their edible bulbs, belonging to the Liliaceae.4-Butyrolactone: One of the FURANS with a carbonyl thereby forming a cyclic lactone. It is an endogenous compound made from gamma-aminobutyrate and is the precursor of gamma-hydroxybutyrate. It is also used as a pharmacological agent and solvent.Anti-Bacterial Agents: Substances that reduce the growth or reproduction of BACTERIA.Biofilms: Encrustations, formed from microbes (bacteria, algae, fungi, plankton, or protozoa) embedding in extracellular polymers, that adhere to surfaces such as teeth (DENTAL DEPOSITS); PROSTHESES AND IMPLANTS; and catheters. Biofilms are prevented from forming by treating surfaces with DENTIFRICES; DISINFECTANTS; ANTI-INFECTIVE AGENTS; and antifouling agents.Genes, rRNA: Genes, found in both prokaryotes and eukaryotes, which are transcribed to produce the RNA which is incorporated into RIBOSOMES. Prokaryotic rRNA genes are usually found in OPERONS dispersed throughout the GENOME, whereas eukaryotic rRNA genes are clustered, multicistronic transcriptional units.Biological Warfare Agents: Living organisms or their toxic products that are used to cause disease or death of humans during WARFARE.Microbial Viability: Ability of a microbe to survive under given conditions. This can also be related to a colony's ability to replicate.Symbiosis: The relationship between two different species of organisms that are interdependent; each gains benefits from the other or a relationship between different species where both of the organisms in question benefit from the presence of the other.Ceftazidime: Semisynthetic, broad-spectrum antibacterial derived from CEPHALORIDINE and used especially for Pseudomonas and other gram-negative infections in debilitated patients.Saccharum: A plant genus of the family POACEAE widely cultivated in the tropics for the sweet cane that is processed into sugar.Nitrogen Fixation: The process in certain BACTERIA; FUNGI; and CYANOBACTERIA converting free atmospheric NITROGEN to biologically usable forms of nitrogen, such as AMMONIA; NITRATES; and amino compounds.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Psychotria: A plant genus of the family RUBIACEAE. Members contain psychotridine and brachycerine (indole alkaloids).Pyrrolnitrin: 3-Chloro-4-(3-chloro-2-nitrophenyl)pyrrole. Antifungal antibiotic isolated from Pseudomonas pyrrocinia. It is effective mainly against Trichophyton, Microsporium, Epidermophyton, and Penicillium.Thailand: Formerly known as Siam, this is a Southeast Asian nation at the center of the Indochina peninsula. Bangkok is the capital city.Virulence Factors: Those components of an organism that determine its capacity to cause disease but are not required for its viability per se. Two classes have been characterized: TOXINS, BIOLOGICAL and surface adhesion molecules that effect the ability of the microorganism to invade and colonize a host. (From Davis et al., Microbiology, 4th ed. p486)Species Specificity: The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.Nucleic Acid Hybridization: Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)Opportunistic Infections: An infection caused by an organism which becomes pathogenic under certain conditions, e.g., during immunosuppression.Sputum: Material coughed up from the lungs and expectorated via the mouth. It contains MUCUS, cellular debris, and microorganisms. It may also contain blood or pus.Burkholderiaceae: A family of gram negative, aerobic, non-sporeforming, rod-shaped bacteria.RNA, Bacterial: Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.ArabinoseCarbon-Carbon Lyases: Enzymes that catalyze the cleavage of a carbon-carbon bond by means other than hydrolysis or oxidation. This subclass contains the DECARBOXYLASES, the ALDEHYDE-LYASES, and the OXO-ACID-LYASES. EC 4.1.Rec A Recombinases: A family of recombinases initially identified in BACTERIA. They catalyze the ATP-driven exchange of DNA strands in GENETIC RECOMBINATION. The product of the reaction consists of a duplex and a displaced single-stranded loop, which has the shape of the letter D and is therefore called a D-loop structure.Electrophoresis, Gel, Pulsed-Field: Gel electrophoresis in which the direction of the electric field is changed periodically. This technique is similar to other electrophoretic methods normally used to separate double-stranded DNA molecules ranging in size up to tens of thousands of base-pairs. However, by alternating the electric field direction one is able to separate DNA molecules up to several million base-pairs in length.

Burkholderia cocovenenans (van Damme et al. 1960) Gillis et al. 1995 and Burkholderia vandii Urakami et al. 1994 are junior synonyms of Burkholderia gladioli (Severini 1913) Yabuuchi et al. 1993 and Burkholderia plantarii (Azegami et al. 1987) Urakami et al. 1994, respectively. (1/537)

Reference strains of Burkholderia cocovenenans and Burkholderia vandii were compared with strains of other Burkholderia species using SDS-PAGE of whole-cell proteins, DNA-DNA hybridization and extensive biochemical characterization. Burkholderia gladioli and B. cocovenenans were indistinguishable in the chemotaxonomic and biochemical analyses. Burkholderia plantarii and B. vandii had indistinguishable whole-cell protein patterns but the B. vandii type strain differed from B. plantarii strains in several biochemical tests. The DNA-DNA binding levels (higher than 70%) indicated that (i) B. gladioli and B. cocovenenans, and (ii) B. plantarii and B. vandii each represent a single species. It is concluded that B. cocovenenans and B. vandii are junior synonyms of B. gladioli and B. plantarii, respectively.  (+info)

All in the family: structural and evolutionary relationships among three modular proteins with diverse functions and variable assembly. (2/537)

The crystal structures of three proteins of diverse function and low sequence similarity were analyzed to evaluate structural and evolutionary relationships. The proteins include a bacterial bleomycin resistance protein, a bacterial extradiol dioxygenase, and human glyoxalase I. Structural comparisons, as well as phylogenetic analyses, strongly indicate that the modern family of proteins represented by these structures arose through a rich evolutionary history that includes multiple gene duplication and fusion events. These events appear to be historically shared in some cases, but parallel and historically independent in others. A significant early event is proposed to be the establishment of metal-binding in an oligomeric ancestor prior to the first gene fusion. Variations in the spatial arrangements of homologous modules are observed that are consistent with the structural principles of three-dimensional domain swapping, but in the unusual context of the formation of larger monomers from smaller dimers or tetramers. The comparisons support a general mechanism for metalloprotein evolution that exploits the symmetry of a homooligomeric protein to originate a metal binding site and relies upon the relaxation of symmetry, as enabled by gene duplication, to establish and refine specific functions.  (+info)

Discrimination of Burkholderia multivorans and Burkholderia vietnamiensis from Burkholderia cepacia genomovars I, III, and IV by PCR. (3/537)

We present a PCR procedure for identification of Burkholderia cepacia, Burkholderia multivorans, and Burkholderia vietnamiensis. 16S and 23S ribosomal DNAs (rDNAs) of B. multivorans and B. vietnamiensis were sequenced and aligned with published sequences for definition of species-specific 18-mer oligonucleotide primers. Specific antisense 16S rDNA primers (for B. cepacia, 5'-AGC ACT CCC RCC TCT CAG-3'; for B. multivorans, 5'-AGC ACT CCC GAA TCT CTT-3') and 23S rDNA primers (for B. vietnamiensis, 5'-TCC TAC CAT GCG TGC AA-3') were paired with a general sense primer of 16S rDNAs (5'-AGR GTT YGA TYM TGG CTC AG-3') or with a sense primer of 23S rDNA (5'-CCT TTG GGT CAT CCT GGA-3'). PCR with these primers under optimized conditions is appropriate to specifically and rapidly identify B. multivorans, B. vietnamiensis, and B. cepacia (genomovars I, III, and IV are not discriminated). In comparison with the polyphasic taxonomic analyses presently necessary for species and genomovar identification within the B. cepacia complex, our procedure is more rapid and easier to perform and may contribute to clarifying the clinical significance of individual members of the complex in cystic fibrosis.  (+info)

Expression and functional analysis of an N-truncated NifA protein of Herbaspirillum seropedicae. (4/537)

In Herbaspirillum seropedicae, an endophytic diazotroph, nif gene expression is under the control of the transcriptional activator NifA. We have over-expressed and purified a protein containing the central and C-terminal domains of the H. seropedicae NifA protein, N-truncated NifA, fused to a His-Tag sequence. This fusion protein was found to be partially soluble and was purified by affinity chromatography. Band shift and footprinting assays showed that the N-truncated NifA protein was able to bind specifically to the H. seropedicae nifB promoter region. In vivo analysis showed that this protein activated the nifH promoter of Klebsiella pneumoniae in Escherichia coli only in the absence of oxygen and this activation was not negatively controlled by ammonium ions.  (+info)

Burkholderia caribensis sp. nov., an exopolysaccharide-producing bacterium isolated from vertisol microaggregates in Martinique. (5/537)

Twenty-one exopolysaccharide-producing strains were isolated from the 5-20 microns fraction of a vertisol in the south-east of the island of Martinique in the French West Indies. Although these strains were phenotypically identified as Burkholderia cepacia or as Burkholderia glathei using BIOLOG microplates, they did not cluster genotypically by amplified rDNA restriction analysis (ARDRA) with any described Burkholderia species. A phylogenetic analysis revealed that the rrs (16S rDNA) sequences of three representative strains clustered in a single branch within the genus Burkholderia and distantly from all of the previously described species of Burkholderia for which rrs sequences were available. DNA-DNA hybridization data as well as phenotypic analyses indicated that the 21 isolates represented a single and new species for which the name Burkholderia caribensis sp. nov. is proposed (type strain MWAP64T = LMG 18531T).  (+info)

Degradation of chlorobenzenes at nanomolar concentrations by Burkholderia sp. strain PS14 in liquid cultures and in soil. (6/537)

The utilization of 1,2,4,5-tetrachloro-, 1,2,4-trichloro-, the three isomeric dichlorobenzenes and fructose as the sole carbon and energy sources at nanomolar concentrations was studied in batch experiments with Burkholderia sp. strain PS14. In liquid culture, all chlorobenzenes were metabolized within 1 h from their initial concentration of 500 nM to below their detection limits of 0.5 nM for 1,2,4,5-tetrachloro- and 1,2,4-trichlorobenzene and 7.5 nM for the three dichlorobenzene isomers, with 63% mineralization of the tetra- and trichloroisomers. Fructose at the same initial concentration was, in contrast, metabolized over a 4-h incubation period down to a residual concentration of approximately 125 nM with 38% mineralization during this time. In soil microcosms, Burkholderia sp. strain PS14 metabolized tetrachlorobenzene present at 64.8 ppb and trichlorobenzene present at 54.4 ppb over a 72-h incubation period to below the detection limits of 0.108 and 0.09 ppb, respectively, with approximately 80% mineralization. A high sorptive capacity of Burkholderia sp. strain PS14 for 1,2,4, 5-tetrachlorobenzene was found at very low cell density. The results demonstrate that Burkholderia sp. strain PS14 exhibits a very high affinity for chlorobenzenes at nanomolar concentrations.  (+info)

Identification of Burkholderia spp. in the clinical microbiology laboratory: comparison of conventional and molecular methods. (7/537)

Cystic fibrosis (CF) predisposes patients to bacterial colonization and infection of the lower airways. Several species belonging to the genus Burkholderia are potential CF-related pathogens, but microbiological identification may be complicated. This situation is not in the least due to the poorly defined taxonomic status of these bacteria, and further validation of the available diagnostic assays is required. A total of 114 geographically diverse bacterial isolates, previously identified in reference laboratories as Burkholderia cepacia (n = 51), B. gladioli (n = 14), Ralstonia pickettii (n = 6), B. multivorans (n = 2), Stenotrophomonas maltophilia (n = 3), and Pseudomonas aeruginosa (n = 11), were collected from environmental, clinical, and reference sources. In addition, 27 clinical isolates putatively identified as Burkholderia spp. were recovered from the sputum of Dutch CF patients. All isolates were used to evaluate the accuracy of two selective growth media, four systems for biochemical identification (API 20NE, Vitek GNI, Vitek NFC, and MicroScan), and three different PCR-based assays. The PCR assays amplify different parts of the ribosomal DNA operon, either alone or in combination with cleavage by various restriction enzymes (PCR-restriction fragment length polymorphism [RFLP] analysis). The best system for the biochemical identification of B. cepacia appeared to be the API 20NE test. None of the biochemical assays successfully grouped the B. gladioli strains. The PCR-RFLP method appeared to be the optimal method for accurate nucleic acid-mediated identification of the different Burkholderia spp. With this method, B. gladioli was also reliably classified in a separate group. For the laboratory diagnosis of B. cepacia, we recommend parallel cultures on blood agar medium and selective agar plates. Further identification of colonies with a Burkholderia phenotype should be performed with the API 20NE test. For final confirmation of species identities, PCR amplification of the small-subunit rRNA gene followed by RFLP analysis with various enzymes is recommended.  (+info)

Differentiation of Burkholderia species by PCR-restriction fragment length polymorphism analysis of the 16S rRNA gene and application to cystic fibrosis isolates. (8/537)

Burkholderia cepacia, which is an important pathogen in cystic fibrosis (CF) owing to the potential severity of the infections and the high transmissibility of some clones, has been recently shown to be a complex of five genomic groups, i.e., genomovars I, II (B. multivorans), III, and IV and B. vietnamiensis. B. gladioli is also involved, though rarely, in CF. Since standard laboratory procedures fail to provide an accurate identification of these organisms, we assessed the ability of restriction fragment length polymorphism (RFLP) analysis of amplified 16S ribosomal DNA (rDNA), with the combination of the patterns obtained with six endonucleases, to differentiate Burkholderia species. This method was applied to 16 type and reference strains of the genus Burkholderia and to 51 presumed B. cepacia clinical isolates, each representative of one clone previously determined by PCR ribotyping. The 12 Burkholderia type strains tested were differentiated, including B. cepacia, B. multivorans, B. vietnamiensis, and B. gladioli, but neither the genomovar I and III reference strains nor the genomovar IV reference strain and B. pyrrociniaT were distinguishable. CF clinical isolates were mainly distributed in RFLP group 2 (which includes B. multivoransT) and RFLP group 1 (which includes B. cepacia genomovar I and III reference strains, as well as nosocomial clinical isolates). Two of the five highly transmissible clones in French CF centers belonged to RFLP group 2, and three belonged to RFLP group 1. The remaining isolates either clustered with other Burkholderia species (B. cepacia genomovar IV or B. pyrrocinia, B. vietnamiensis, and B. gladioli) or harbored unique combinations of patterns. Thus, if further validated by hybridization studies, PCR-RFLP of 16S rDNA could be an interesting identification tool and contribute to a better evaluation of the respective clinical risks associated with each Burkholderia species or genomovar in patients with CF.  (+info)

Pathogenic Burkholderia rely on host factors for efficient intracellular replication and are highly refractory to antibiotic treatment. To identify host genes that are required by Burkholderia spp. during infection, we performed a RNA interference (RNAi) screen of the human kinome and identified 35 host kinases that facilitated Burkholderia thailandensis intracellular survival in human monocytic THP-1 cells. We validated a selection of host kinases using imaging flow cytometry to assess efficiency of B. thailandensis survival in the host upon siRNA-mediated knockdown. We focused on the role of the novel protein kinase C isoform, PKC-η, in Burkholderia infection and characterized PKC-η/MARCKS signaling as a key event that promotes the survival of unopsonized B. thailandensis CDC2721121 within host cells. While infection of lung epithelial cells with unopsonized Gram-negative bacteria stimulated phosphorylation of Ser175/160 in the MARCKS effector domain, siRNA-mediated knockdown of PKC-η ...
ID CP000871; SV 1; circular; genomic DNA; STD; PRO; 167422 BP. XX AC CP000871; AAVB01000000-AAVB01000037; XX PR Project:PRJNA17407; XX DT 16-NOV-2007 (Rel. 93, Created) DT 12-DEC-2013 (Rel. 119, Last updated, Version 2) XX DE Burkholderia multivorans ATCC 17616 plasmid pBMUL01, complete sequence. XX KW . XX OS Burkholderia multivorans ATCC 17616 OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; OC Burkholderiaceae; Burkholderia; Burkholderia cepacia complex. OG Plasmid pBMUL01 XX RN [1] RP 1-167422 RG US DOE Joint Genome Institute RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., RA Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., RA Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Tiedje J., RA Richardson P.; RT "Complete sequence of plasmid1 of Burkholderia multivorans ATCC 17616"; RL Unpublished. XX RN [2] RP 1-167422 RG US DOE Joint Genome Institute RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., ...
Burkholderia thailandensis is a non-pathogenic environmental saprophyte closely related to Burkholderia pseudomallei, the causative agent of the often fatal animal and human disease melioidosis. To study B. thailandensis genomic variation, we profiled 50 isolates using a pan-genome microarray comprising genomic elements from 28 Burkholderia strains and species. Of 39 genomic regions variably present across the B. thailandensis strains, 13 regions corresponded to known genomic islands, while 26 regions were novel. Variant B. thailandensis isolates exhibited isolated acquisition of a capsular polysaccharide biosynthesis gene cluster (B. pseudomallei-like capsular polysaccharide) closely resembling a similar cluster in B. pseudomallei that is essential for virulence in mammals; presence of this cluster was confirmed by whole genome sequencing of a representative variant strain (B. thailandensis E555). Both whole-genome microarray and multi-locus sequence typing analysis revealed that the variant strains
Product information: Burkholderia ambifaria Putative membrane protein insertion efficiency factor BamMC406_3100 - Gentaur.com - Product info
Cystic fibrosis (CF) is a recessive genetic disease characterized by chronic respiratory infections and inflammation causing permanent lung damage. Recurrent infections are caused by Gram-negative antibiotic-resistant bacterial pathogens such as Pseudomonas aeruginosa, Burkholderia cepacia complex (Bcc) and the emerging pathogen genus Pandoraea. In this study, the interactions between co-colonizing CF pathogens were investigated. Both Pandoraea and Bcc elicited potent pro-inflammatory responses that were significantly greater than Ps. aeruginosa. The original aim was to examine whether combinations of pro-inflammatory pathogens would further exacerbate inflammation. In contrast, when these pathogens were colonized in the presence of Ps. aeruginosa the pro-inflammatory response was significantly decreased. Real-time PCR quantification of bacterial DNA from mixed cultures indicated that Ps. aeruginosa significantly inhibited the growth of Burkholderia multivorans, Burkholderia cenocepacia, Pandoraea
Members of the Burkholderia cepacia complex (Bcc), such as B. ambifaria, are effective biocontrol strains, for instance, as plant growth-promoting bacteria; however, Bcc isolates can also cause severe respiratory infections in people suffering from cystic fibrosis (CF). No distinction is known between isolates from environmental and human origins, suggesting that the natural environment is a potential source of infectious Bcc species. While investigating the presence and role of phase variation in B. ambifaria HSJ1, an isolate recovered from a CF patient, we identified stable variants that arose spontaneously irrespective of the culture conditions. Phenotypic and proteomic approaches revealed that the transition from wild-type to variant types affects the expression of several putative virulence factors. By using four different infection models (Drosophila melanogaster, Galleria mellonella, macrophages and Dictyostelium discoideum), we showed that the wild-type was more virulent than the ...
DNA mismatch repair (MMR) is a highly conserved biological pathway that plays a key role in maintaining genomic stability. MMR corrects DNA mismatches generated during DNA replication, thereby preventing mutations from becoming permanent in dividing cells. MMR also suppresses homologous recombination and was recently shown to play a role in DNA damage signaling. Defects in MMR are associated with genome-wide instability, predisposition to certain types of cancer including HNPCC, resistance to certain chemotherapeutic agents, and abnormalities in meiosis and sterility in mammalian systems. The Escherichia coli MMR pathway has been extensively studied and is well characterized. In E. coli, the mismatch-activated MutS-MutL-ATP complex licenses MutH to incise the nearest unmethylated GATC sequence. UvrD and an exonuclease generate a gap. This gap is filled by pol III and DNA ligase. The GATC sites are then methylated by Dam. Several human MMR proteins have been identified based on their homology to ...
A polyphasic taxonomic study including DNA-DNA reassociation experiments and an extensive biochemical characterization was performed on 14 Burkholderia isolates from moss gametophytes of nutrient-poor plant communities on the southern Baltic Sea coast in northern Germany. The strains were classified within two novel species, for which the names Burkholderia bryophila sp. nov. and Burkholderia megapolitana sp. nov. are proposed. The former species also includes isolates from grassland and agricultural soil collected in previous studies. Strains Burkholderia bryophila 1S18T (=LMG 23644T =CCUG 52993T) and Burkholderia megapolitana A3T (=LMG 23650T =CCUG 53006T) are the proposed type strains. They were isolated from Sphagnum rubellum and Aulacomnium palustre, respectively, growing in the Ribnitzer Großes Moor nature reserve (Mecklenburg-Pommern, Germany). All moss isolates of both novel species showed antifungal activity against phytopathogens as well as plant-growth-promoting properties.
Chebbi, Alif, Elshikh, Mohamed, Haque, Farazul, Ahmed, Syed, Dobbin, Sara, Marchant, R, Sayadi, Sami, Chamkha, Mohamed and Banat, Ibrahim M (2017) Rhamnolipids from Pseudomonas aeruginosa strain W10;as antibiofilm/antibiofouling products for metal protection. Journal of Basic Microbiology, 9999 . pp. 1-12. [Journal article] Elshikh, M, Moya-Ramirez, I, Moens, H, Roelants, S, Soetaert, W, Marchant, Roger and Banat, Ibrahim M (2017) Rhamnolipids and Lactonic Sophorolipids: Natural antimicrobial surfactants for oral hygiene. Journal of Applied Microbiology, 123 . [Journal article] Elshikh, Mohamed, Funston, Scott, chebbi, Alif, Ahmed, Syed, Marchant, R and Banat, Ibrahim M (2017) Rhamnolipids from non-pathogenic Burkholderia thailandensis E264: Physicochemical characterization, antimicrobial and antibiofilm efficacy against oral hygiene related pathogens. New Biotechnology, 36 . pp. 26-36. [Journal article] Funston, SJ, Tsaousi, Konstantina, Smyth, TJ, Twigg, Matthew, Marchant, Roger and Banat, ...
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p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
In bacterial genomes composed of more than one chromosome, one replicon is typically larger, harbors more essential genes than the others, and is considered primary. The greater variability of secondary chromosomes among related taxa has led to the theory that they serve as an accessory genome for specific niches or conditions. By this rationale, purifying selection should be weaker on genes on secondary chromosomes because of their reduced necessity or usage. To test this hypothesis we selected bacterial genomes composed of multiple chromosomes from two genera, Burkholderia and Vibrio, and quantified the evolutionary rates (dN and dS) of all orthologs within each genus. Both evolutionary rate parameters were faster among orthologs found on secondary chromosomes than those on the primary chromosome. Further, in every bacterial genome with multiple chromosomes that we studied, genes on secondary chromosomes exhibited significantly weaker codon usage bias than those on primary chromosomes. Faster
ID B2TBM3_PARPJ Unreviewed; 220 AA. AC B2TBM3; DT 01-JUL-2008, integrated into UniProtKB/TrEMBL. DT 01-JUL-2008, sequence version 1. DT 25-OCT-2017, entry version 53. DE SubName: Full=Glutathione S-transferase domain {ECO:0000313,EMBL:ACD19675.1}; GN OrderedLocusNames=Bphyt_5316 {ECO:0000313,EMBL:ACD19675.1}; OS Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN) OS (Burkholderia phytofirmans). OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; OC Burkholderiaceae; Paraburkholderia. OX NCBI_TaxID=398527 {ECO:0000313,EMBL:ACD19675.1, ECO:0000313,Proteomes:UP000001739}; RN [1] {ECO:0000313,EMBL:ACD19675.1, ECO:0000313,Proteomes:UP000001739} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=DSM 17436 / LMG 22146 / PsJN RC {ECO:0000313,Proteomes:UP000001739}; RX PubMed=21551308; DOI=10.1128/JB.05055-11; RA Weilharter A., Mitter B., Shin M.V., Chain P.S., Nowak J., RA Sessitsch A.; RT "Complete genome sequence of the plant growth-promoting endophyte RT ...
Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.
Accepted name: 2,4-dinitrotoluene dioxygenase. Reaction: 2,4-dinitrotoluene + NADH + O2 = 4-methyl-5-nitrocatechol + nitrite + NAD+. Other name(s): dntA (gene name). Systematic name: 2,4-dinitrotoluene,NADH:oxygen oxidoreductase (4,5-hydroxylating, nitrite-releasing). Comments: This enzyme, characterized from the bacterium Burkholderia sp. strain DNT, is a member of the naphthalene family of bacterial Rieske non-heme iron dioxygenases. It comprises a multicomponent system, containing a Rieske [2Fe-2S] ferredoxin, an NADH-dependent flavoprotein reductase (EC 1.18.1.3, ferredoxin NAD+ reductase), and an α3β3 oxygenase. The enzyme forms a cis-dihydroxylated product that spontaneously rearranges to form a catechol with accompanying release of nitrite. It does not act on nitrobenzene. cf. EC 1.14.12.23, nitroarene dioxygenase.. Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: References:. 1. Suen, W.C., Haigler, B.E. and Spain, J.C. 2,4-Dinitrotoluene dioxygenase from ...
We are studying a representative of a large group of chlorobenzoate degrading bacteria active in uncontaminated systems - Burkholderia phytofirmans OLGA172. Now we know that anthropogenic chemicals have not always been the driving selective force behind the evolution of chloro-organic degradative genes, we are looking for the natural selectors for these capabilities, initially by looking at the environmental triggers and mobile genetic elements that affect chloroaromatic degradative gene expression. The recent genome sequencing of OLGA172 in the lab has expanded this endeavour.. In addition, my lab carries out research on the species composition of bacterial communities in both natural and contaminated systems. These include waste water treatment systems, contaminated soils, rhizospheric communities and the hyporheic zones of streams.. The UTSC campus is heavily infested with an invasive species known as Dog Strangling Vine. My lab has recently begun research into the microbial associations of ...
Species Fungorum synonymy Index Fungorum LSID: urn:lsid:indexfungorum.org:names:438420; click here to update this record. Index Fungorum UUID: {B50B9A36-FD1F-40F5-95E8-9DB182D3CF41} Please contact Paul Kirk if you have any additions or errors to report. Data contributors.. back to previous page. ...
Species Fungorum synonymy Index Fungorum LSID: urn:lsid:indexfungorum.org:names:110903; click here to update this record. Index Fungorum UUID: {72CA4BF6-B31D-455F-A5DC-5D1C09090735} Please contact Paul Kirk if you have any additions or errors to report. Data contributors.. back to previous page. ...
Tuanyok, Apichai; Mayo, Mark; Scholz, Holger; Hall, Carina M.; Allender, Christopher J.; Kaestli, Mirjam; Ginther, Jennifer; Spring-Pearson, Senanu; Bollig, Molly C.; Stone, Joshua K.; Settles, Erik W.; Busch, Joseph D.; Sidak-Loftis, Lindsay; Sahl, Jason W.; Thomas, Astrid; Kreutzer, Lisa; Georgi, Enrico; Gee, Jay E.; Bowen, Richard A.; Ladner, Jason T.; Lovett, Sean; Koroleva, Galina; Palacios, Gustavo; Wagner, David M.; Currie, Bart J.; Keim, Paul ...
Burkholderia cepacia is a gram negative bacilli usually isolated from persons with genetic lung diseases such as Cystic fibrosis. It was discovered as a plant pathogen but later researchers have found it to be a notorious pathogen in humans. It is also a nosocomial pathogen which contaminates equipment and even disinfectants. In the other end of the spectrum, research suggests that these organisms maybe used as bio diesel and also as pesticides and fertilizers. This review of literature gives an idea of the organism and the gateway it opens for further research with a special note on the evolution of this genus as a nosocomial infection in Neonates.
Hong-Geller E. (2013) Small-RNA-mediated regulation of host-pathogen interactions. Virulence. In preparation.. Stubben CJ, Micheva-Viteva S, Shou Y, Dunbar JM, and Hong-Geller E. (2013) Differential expression of small RNAs from Burkholderia thailandensis in response to environmental and stressful growth conditions. In preparation.. Shepherd DP, Li N, Micheva-Viteva S, Munsky B, Hong-Geller E, and Werner J. (2013) Counting small RNA in pathogenic bacteria. Submitted.. Micheva-Viteva S, Shou Y, Nowak-Lovato K, Rector KD, and Hong-Geller E. (2013) c-KIT-EGR1 signaling is targeted by Yersinia during infection. Submitted.. Nowak-Lovato K, Alexandrov LB, Banisadr A, Bauer AL, Bishop AR, Usheva A, Mu F, Hong-Geller E, Rasmussen K, Hlavacek WS, and Alexandrov BS. (2013) Binding of Nucleoid-associated Protein Fis to DNA is Regulated by DNA Breathing Dynamics. PLoS Comp. Biol. Jan;9(1):e1002881. doi: 10.1371/journal.pcbi.1002881.. Marti-Arbona R, Teshima M, Anderson PS, Nowak-Lovato K, Hong-Geller E, ...
Infections and inflammation can lead to cachexia and wasting of skeletal muscle and fat tissue by as yet poorly understood mechanisms. We observed that gut colonization of mice by a strain of Escherichia coli prevents wasting triggered by infections or physical damage to the intestine. During intestinal infection with the pathogen Salmonella Typhimurium or pneumonic infection with Burkholderia thailandensis, the presence of this E. coli did not alter changes in host metabolism, caloric uptake, or inflammation but instead sustained signaling of the insulin-like growth factor 1/phosphatidylinositol 3-kinase/AKT pathway in skeletal muscle, which is required for prevention of muscle wasting. This effect was dependent on engagement of the NLRC4 inflammasome. Therefore, this commensal promotes tolerance to diverse diseases. ...
Bacterial persistence, known as noninherited antibacterial resistance, is a factor contributing to the establishment of long-lasting chronic bacterial infections. In this study, we examined the ability of nicotinamide (NA) to potentiate the activity of different classes of antibiotics against Burkholderia thailandensis persister cells. ...
The goal of this proposal is to demonstrate that colocalization of protein subunit antigens and adjuvants on nanolipoprotein particles (NLPs) can increase the protective efficacy of subunit antigens from Burkholderia spp. and Francisella tularensis against an aerosol challenge. In the second quarter of the third year, LLNL finalized all immunological assessments of NLP vaccine formulations in the F344 model. Battelle has immunized rats with three unique NLP formulations by either intramuscular or intranasal administration. All inoculations have been completed, and protective efficacy against an aerosolized challenge will begin at the end of October, 2014. ...
Clavariaceae, Agaricales, Agaricomycetidae, Agaricomycetes, Basidiomycota, Fungi. Index Fungorum LSID: urn:lsid:indexfungorum.org:names:246188 Please contact Paul Kirk if you have any additions or errors to report. Data contributors.. back to previous page. ...
display synonymy Index Fungorum LSID: urn:lsid:indexfungorum.org:names:256707 Please contact Paul Kirk if you have any additions or errors to report. Data contributors.. back to previous page. ...
Kirk, Paul M. (2017). Anaptychia runcinata (With.) J.R. Laundon, 1984. In: Index Fungorum Partnership (2017). Index Fungorum. Accessed through: World Register of Marine Species at http://www.marinespecies.org/aphia.php?p=taxdetails&id=147742 on 2017-12- ...
Kirk, Paul M. (2009). Biatriospora. In: Index Fungorum Partnership (2017). Index Fungorum. Accessed through: World Register of Marine Species at http://www.marinespecies.org/aphia.php?p=taxdetails&id=147693 on 2017-12- ...
Acclimation, Cold, Plant, Temperatures, Bacteria, Burkholderia, Gene, Growth, Plants, Temperature, Fructose, Mannose, Metabolism, Starch, Water, Gene Expression, Mold, Time, Tissues, Affects
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マイクロプレートリーダー,ベクレルモニター,ガンマ線スペクトロメーター,(LB200/LB2045),マイクロプレートリーダー,ベクレルモニター,ガンマ線スペクトロメーター
The trend to search novel microbial natural biocides has recently been increasing in order to avoid the environmental pollution from use of synthetic pesticides. Among these novel natural biocides are the bioactive secondary metabolites of Burkholderia gladioli pv. agaricicola (Bga). The aim of this study is to determine antifungal activity of Bga strains against some phytopathogenic fungi. The fungicidal tests were carried out using cultures and cell-free culture filtrates against Botrytis cinerea, Aspergillus flavus, Aspergillus niger, Penicillium digitatum, Penicillium expansum, Sclerotinia sclerotiorum and Phytophthora cactorum. Results demonstrated that all tested strains exert antifungal activity against all studied fungi by producing diffusible metabolites which are correlated with their ability to produce extracellular hydrolytic enzymes. All strains significantly reduced the growth of studied fungi and the bacterial cells were more bioactive than bacterial filtrates. All tested Bulkholderia
Wallqvist, A., H. Wang, N. Zavaljevski, V. Memisevic, K. Kwon, R. Pieper, S. V. Rajagopala, and J. Reifman. Mechanisms of action of Coxiella burnetii effectors inferred from host-pathogen protein interactions. PLOS ONE. 2017 November 27; 12(11):e0188071. [PDF]. Memisevic, V., K. Kumar, N. Zavaljevski, D. DeShazer, A. Wallqvist, and J. Reifman. DBSecSys 2.0: a database of Burkholderia mallei and Burkholderia pseudomallei secretion systems. BMC Bioinformatics. 2016 September 20; 17:387. [PDF]. Wallqvist, A., V. Memisevic, N. Zavaljevski, R. Pieper, S. V. Rajagopala, K. Kwon, C. Yu, T. A. Hoover, and J. Reifman. Using host-pathogen protein interactions to identify and characterize Francisella tularensis virulence factors. BMC Genomics. 2015 December 29; 16:1106. [PDF]. Chiang, C. Y., I. Uzoma, D. J. Lane, V. Memisevic, F. Alem, K. Yao, S. Bavari, A. Wallqvist, R. M. Hakami, and R. G. Panchal. A reverse-phase protein microarray-based screen identifies host signaling dynamics upon Burkholderia spp. ...
The bacterium Burkholderia multivorans evolves and adapts in bursts to survive in the lungs of cystic fibrosis patients, according to a study published in...
Quorum sensing (QS) is a cell density-dependent mechanism which enables a population of bacteria to coordinate cooperative behaviors in response to the accumulation of self-produced autoinducer signals in their local environment. An emerging framework is that the adaptive significance of QS in the regulation of production of costly extracellular metabolites («public goods») is to maintain the homeostasis of cooperation. We investigated this model using the phytopathogenic bacterium Burkholderia glumae, which we have previously demonstrated uses QS to regulate the production of rhamnolipids, extracellular surface-active glycolipids promoting the social behavior called «swarming motility». Using mass spectrometric quantification and chromosomal lux-based gene expression, we made the unexpected finding that when unrestricted nutrient resources are provided, production of rhamnolipids is carried out completely independently of QS regulation. This is a unique observation among known QS-controlled factors
We generated a Burkholderia mutant, which is deficient of an N-acetylmuramyl-l-alanine amidase, AmiC, involved in peptidoglycan degradation. When non-motile ΔamiC mutant Burkholderia cells harboring chain form were orally administered to Riptortus insects, ΔamiC mutant cells were unable to establish symbiotic association. But, ΔamiC mutant complemented with amiC gene restored in vivo symbiotic association. ΔamiC mutant cultured in minimal medium restored their motility with single-celled morphology. When ΔamiC mutant cells harboring single-celled morphology were administered to the host insect, this mutant established normal symbiotic association, suggesting that bacterial motility is essential for the successful symbiosis between host insect and Burkholderia symbiont ...
Molecular identification of bioterrorism agents such as B. anthracis, Brucella spp., Burkholderia spp., Ricin toxin, F. tularensis, Y. pestis, Varicella and Vaccinia virus is currently performed using real-time PCR and EIA protocols developed and distributed by the Laboratory Response Network of the Centers for Disease Control and Prevention (CDC). Our laboratory also performs MSDH-designed assays for the molecular detection of the following infectious agents: Bordetella species, Norovirus, Measles, Mumps, and Shiga-Toxin producing E. coli. Molecular subtyping of the food-borne disease-causing bacteria Salmonella, Shigella, Listeria and E. coli are also performed by our laboratory using pulse-field electrophoresis (PFGE) and is coordinated through PulseNet, the CDCs national foodborne disease detection network. This section also performs influenza A and B virus testing, influenza A subtyping, and influenza B genotyping primarily for surveillance purposes. Results are used to monitor state ...
Molecular identification of bioterrorism agents such as B. anthracis, Brucella spp., Burkholderia spp., Ricin toxin, F. tularensis, Y. pestis, Varicella and Vaccinia virus is currently performed using real-time PCR and EIA protocols developed and distributed by the Laboratory Response Network of the Centers for Disease Control and Prevention (CDC). Our laboratory also performs MSDH-designed assays for the molecular detection of the following infectious agents: Bordetella species, Norovirus, Measles, Mumps, and Shiga-Toxin producing E. coli. Molecular subtyping of the food-borne disease-causing bacteria Salmonella, Shigella, Listeria and E. coli are also performed by our laboratory using pulse-field electrophoresis (PFGE) and is coordinated through PulseNet, the CDCs national foodborne disease detection network. This section also performs influenza A and B virus testing, influenza A subtyping, and influenza B genotyping primarily for surveillance purposes. Results are used to monitor state ...
Molecular identification of bioterrorism agents such as B. anthracis, Brucella spp., Burkholderia spp., Ricin toxin, F. tularensis, Y. pestis, Varicella and Vaccinia virus is currently performed using real-time PCR and EIA protocols developed and distributed by the Laboratory Response Network of the Centers for Disease Control and Prevention (CDC). Our laboratory also performs MSDH-designed assays for the molecular detection of the following infectious agents: Bordetella species, Norovirus, Measles, Mumps, and Shiga-Toxin producing E. coli. Molecular subtyping of the food-borne disease-causing bacteria Salmonella, Shigella, Listeria and E. coli are also performed by our laboratory using pulse-field electrophoresis (PFGE) and is coordinated through PulseNet, the CDCs national foodborne disease detection network. This section also performs influenza A and B virus testing, influenza A subtyping, and influenza B genotyping primarily for surveillance purposes. Results are used to monitor state ...
The enzyme from Burkholderia fungorum strain LB400 (previously Pseudomonas sp.) is part of a multicomponent system composed of an NADH:ferredoxin oxidoreductase (FAD cofactor), a [2Fe-2S] Rieske-type ferredoxin, and a terminal oxygenase that contains a [2Fe-2S] Rieske- type iron-sulfur cluster and a catalytic mononuclear nonheme iron center. -!- Chlorine-substituted biphenyls can also act as substrates. -!- Similar to the three-component enzyme systems EC 1.14.12.3 and EC 1.14.12.11 ...
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Patients, Antibiotics, Bacteria, Biofilm, Biofilms, Cystic Fibrosis, Fibrosis, Sputum, Adults, Asthma, Death, Lung, Azithromycin, Burkholderia, Burkholderia Cepacia, Pseudomonas, Pseudomonas Aeruginosa, Rifampin, Treatment, Algorithm
lipopolysaccharide β-1,4-galactosyltransferase (EC 2.4.1.-); β-1,3-glucosyltransferase (EC 2.4.1.-); β-1,2-glucosyltransferase (EC 2.4.1.-); β-1,2-galactosyltransferase (EC 2.4.1.-); LPS β-1,4-galactosyltransferase (EC 2.4.1.-); occidiofungin β-xylosyltransferase (EC 2.4.2.-); UDP-Gal:procollagen β-galactosyltransferase (EC 2.4.1.50 ...
ID Q13S50_PARXL Unreviewed; 203 AA. AC Q13S50; DT 22-AUG-2006, integrated into UniProtKB/TrEMBL. DT 22-AUG-2006, sequence version 1. DT 30-AUG-2017, entry version 79. DE SubName: Full=Two component transcriptional regulator, LuxR family {ECO:0000313,EMBL:ABE33089.1}; GN ORFNames=Bxe_B2906 {ECO:0000313,EMBL:ABE33089.1}; OS Paraburkholderia xenovorans (strain LB400). OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; OC Burkholderiaceae; Paraburkholderia. OX NCBI_TaxID=266265 {ECO:0000313,EMBL:ABE33089.1, ECO:0000313,Proteomes:UP000001817}; RN [1] {ECO:0000313,EMBL:ABE33089.1, ECO:0000313,Proteomes:UP000001817} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=LB400 {ECO:0000313,EMBL:ABE33089.1, RC ECO:0000313,Proteomes:UP000001817}; RX PubMed=17030797; DOI=10.1073/pnas.0606924103; RA Chain P.S.G., Denef V.J., Konstantinidis K.T., Vergez L.M., Agullo L., RA Reyes V.L., Hauser L., Cordova M., Gomez L., Gonzalez M., Land M., RA Lao V., Larimer F., LiPuma J.J., ...
Ah, yes! Im dealing with Burkholderia. 16s rDNA analyses showed similarity to B. cepacia and B. cenocepacia. Perhaps the fact that B. cepacia exists as a complex of many strains (genomovars) that makes biochemical identification inaccurate; what more when I did it the conventional way. Hoping this time that the commercial kit like API could back the 16s analyses up.. ...
Two Burkholderia strains 8111 and 8201 were isolated from root nodules of Dalbergia odorifera, an endemic woody legume in southern China. Phylogenetic analysis of 16S rRNA gene and 16S-23S gene intergenic spacer (ITS) showed that these two bacterial strains were closely related to Burkholderia cepacia and they were also similar in carbon source utilization using Biolog GN2 plate tests. The DNA G+C content of strains 8111 and 8201 were 65.8 and 65.5 mol%. Inoculation tests demonstrated that both strains 8111 and 8201 formed functional root nodules in their original host D. odorifera, and significantly enhanced plant growth (as measured by plant biomass and nitrogen content), compared to the no-inoculated control plants. Sequencing of 16S rRNA gene in nodules of D. odorifera seedlings inoculated with strains 8111 and 8201 confirmed their identity. However, these two strains did not induce root nodulation in Acacia auriculiformis and Erythrophleum fordii. This implies that the nodulation capacity between
Burkholderia pseudomallei, the etiological agent of melioidosis, is a saprophytic bacterium existing endemically in the water and soil of SE Asia and Northern Australia. This organism has shown the ability to remain dormant in its host for decades. B. thailandensis is a closely related non-pathogenic near neighbor that is also found in these soils. It has been suggested that free-living amoeba could be natural reservoirs for these organisms. The interactions of Burkholderia species and Acanthamoeba castellanii, a species of free-living amoeba, were studied to better understand the natural ecology of these organisms and to determine the effects amoeba interactions might have on pathogenesis. In this study, the adherence and persistence of several B. pseudomallei clinical isolates were compared to that of B. thailandensis within both amoeba and a human monocyte cell line. Results showed that B. pseudomallei isolates can enter amoeba and survive therein at varying levels of efficiency. Some isolates were
Glanders is a disease of Equidae caused by Burkholderia mallei, which also has zoonotic potential: Apart from humans, wildcats (zoos!), camels, bears, wolves and dogs are susceptible as well. Cattle, sheep and swine are resistant. The course of the disease is either acute (especially in donkeys and mules) with high fever and respiratory symptoms and death after a few days. Or it is rather chronic, particularly in horses, with nodules and ulcerations on the skin, the mucous membrane and the inner organs. Chronically and subclinically infected animals represent a dangerous source of infection. All secretions of the respiratory tract and the skin are infectious; the incubation period ranges from a few days to many months. In Germany, glanders is considered eradicated, but it does occur in different Asian, African and South American countries (export-relevant test).. In Germany, there is an obligation to inform the authorities!. ...
TY - JOUR. T1 - Epidemiological tracking and population assignment of the non-clonal bacterium, burkholderia pseudomallei. AU - Dale, Julia. AU - Price, Erin P.. AU - Hornstra, Heidie. AU - Busch, Joseph D.. AU - Mayo, Mark. AU - Godoy, Daniel. AU - Wuthiekanun, Vanaporn. AU - Baker, Anthony. AU - Foster, Jeffrey T. AU - Wagner, David M. AU - Tuanyok, Apichai. AU - Warner, Jeffrey. AU - Spratt, Brian G.. AU - Peacock, Sharon J.. AU - Currie, Bart J.. AU - Keim, Paul S. AU - Pearson, Talima R. PY - 2011/12. Y1 - 2011/12. N2 - Rapid assignment of bacterial pathogens into predefined populations is an important first step for epidemiological tracking. For clonal species, a single allele can theoretically define a population. For non-clonal species such as Burkholderia pseudomallei, however, shared allelic states between distantly related isolates make it more difficult to identify population defining characteristics. Two distinct B. pseudomallei populations have been previously identified using ...
Cystic fibrosis (CF) is a genetic disorder characterized by progressive lung function decline. CF patients are at an increased risk of respiratory infections, including those by the environmental bacterium Burkholderia pseudomallei, the causative agent of melioidosis. Here, we compared the genomes of B. pseudomallei isolates collected between similar to 4 and 55 months apart from seven chronically infected CF patients. Overall, the B. pseudomallei strains showed evolutionary patterns similar to those of other chronic infections, including emergence of antibiotic resistance, genome reduction, and deleterious mutations in genes involved in virulence, metabolism, environmental survival, and cell wall components. We documented the first reported B. pseudomallei hypermutators, which were likely caused by defective MutS. Further, our study identified both known and novel molecular mechanisms conferring resistance to three of the five clinically important antibiotics for melioidosis treatment. Our ...
TY - JOUR. T1 - Burkholderia pseudomallei Antibodies in Individuals Living in Endemic Regions in Northeastern Brazil. AU - Rolim, D.B.. AU - Vilar, D.C.F.L.. AU - De Goes Cavalcanti, L.P.. AU - Freitas, C.L.B.N.. AU - Inglis, Tim. AU - Nobre Rodrigues, J.L.N.. AU - Nagao-Dias, A.T.. PY - 2011. Y1 - 2011. N2 - A seroepidemiological investigation was conducted among the population of two municipalities in Northeastern Brazil. Immunoglobulin M (IgM) and IgG antibodies to Burkholderia pseudomallei were positive in 51.27% (161 in 317 samples) and 58.49% (186), respectively. IgM titers were higher in children than in adults. On the contrary, IgG increased progressively with age. We observed a significant association between agricultural occupation and raised IgM titers (P ,0.005) and IgG titers (P ,0.001), and between construction workers and raised IgG titers (P = 0.005). Antibody IgG avidities did not correlate with age. The highest titers of antibodies (1/800) showed the highest antibody avidity ...
Easton A, Haque https://www.ncbi.nlm.nih.gov/pubmed/?term=19829050%5Bpmid%5DA, Chu K, Lukaszewski R, Bancroft GJ. 2007. A critical role for neutrophils in resistance to experimental infection with Burkholderia pseudomallei. J Infect Dis 195: 99-107. A Critical Role for Neutrophils in Resistance to Experimental Infection with Burkholderia pseudomallei. ...
Results During the 3-year study period, B. gladioli was isolated from blood cultures of 14 patients (3.7 per 1000 admissions). Five out of 14 (35.7%) cases have a positive blood culture at the time of initial admission. Primary diagnoses of neonates were severe major congenital anomalies, congenital leukemia, prematurity with respiratory distress syndrome, pneumonia and parapneumonic pleural effusion. Eleven of the 14 patients (78.6%) had undergone at least one invasive procedure and 71.4% of the patients had undergone two or more of invasive procedures. The most susceptible antimicrobial agents were amikacin, gentamicin, imipenem, ciprofloxacin, trimethoprime/sulphametaxazole and ceftriaxone. The overall in-hospital mortality rate was 21.4%.The mortality rate was 7% for B. gladioli infections.. ...
Glanders (from Middle English glaundres or Old French glandres, both meaning glands) (Latin: malleus German: Rotz) (also known as "equinia," "farcy," and "malleus") is an infectious disease that occurs primarily in horses, mules, and donkeys. It can be contracted by other animals, such as dogs, cats and goats. It is caused by infection with the bacterium Burkholderia mallei, usually by ingestion of contaminated food or water. Signs of glanders include the formation of nodular lesions in the lungs and ulceration of the mucous membranes in the upper respiratory tract. The acute form results in coughing, fever, and the release of an infectious nasal discharge, followed by septicaemia and death within days. In the chronic form, nasal and subcutaneous nodules develop, eventually ulcerating. Death can occur within months, while survivors act as carriers.. Glanders is endemic in Africa, Asia, the Middle East, and Central and South America. It has been eradicated from North America, Australia, and most ...
Background Burkholderia pseudomallei is the causative agent of melioidosis, a disease of significant morbidity and mortality in both human and animals in endemic areas. There is no vaccine towards the bacterium available in the market, and the efficacy of many of the bacteriums surface and secreted proteins are currently being evaluated as vaccine candidates. Methodology/Principal Findings With the availability of the B. pseudomallei whole genome sequence, we undertook to identify genes encoding the known immunogenic outer membrane protein A (OmpA). Twelve OmpA domains were identified and ORFs containing these domains were fully annotated. Of the 12 ORFs, two of these OmpAs, Omp3 and Omp7, were successfully cloned, expressed as soluble protein and purified. Both proteins were recognised by antibodies in melioidosis patients sera by Western blot analysis. Purified soluble fractions of Omp3 and Omp7 were assessed for their ability to protect BALB/c mice against B. pseudomallei infection. Mice were
Burkholderia pseudomallei (also known as Pseudomonas pseudomallei) is a Gram-negative, bipolar, aerobic, motile rod-shaped bacterium. It is a soil-dwelling bacterium endemic in tropical and subtropical regions worldwide, particularly in Thailand and northern Australia. It infects humans and animals and causes the disease melioidosis. It is also capable of infecting plants. B. pseudomallei measures 2-5 μm in length and 0.4-0.8 μm in diameter and is capable of self-propulsion using flagella. The bacteria can grow in a number of artificial nutrient environments, especially betaine- and arginine-containing ones. In vitro, optimal proliferation temperature is reported around 40 °C in neutral or slightly acidic environments (pH 6.8-7.0). The majority of strains are capable of fermentation of sugars without gas formation (most importantly, glucose and galactose; older cultures are reported to also metabolize maltose and starch). Bacteria produce both exo- and endotoxins. The role of the toxins ...
Burkholderia pseudomallei is a human and animal pathogen in tropical regions, especially Southeast Asia and northern Australia. Currently little is known about the genetics and molecular biology of this organism. In this report, we describe the mutagenesis of B. pseudomallei with the transposon Tn5-OT182. B. pseudomallei 1026b transposon mutants were obtained at a frequency of 4.6 x 10(-4) per initial donor cell, and the transposon inserted randomly into the chromosome. We used Tn5-OT182 to identify the flagellin structural gene, fliC. We screened 3,500 transposon mutants and identified 28 motility mutants. Tn5-OT182 integrated into 19 unique genetic loci encoding proteins with homology to Escherichia coli and Salmonella typhimurium flagellar and chemotaxis proteins. Two mutants, MM35 and MM36, contained Tn5-OT182 integrations in fliC. We cloned and sequenced fliC and used it to complement MM35 and MM36 in trans. The fliC transcriptional start site and a sigmaF-like promoter were identified by ...
Burkholderia pseudomallei is resistant to a diverse group of antimicrobials including third generation cephalosporins whilst quinolones and aminoglycosides have no reliable effect. As therapeutic options are limited, development of more effective forms of immunotherapy is vital to avoid a fatal outcome. In an earlier study, we reported on the B. pseudomallei serine MprA protease which is relatively stable over a wide pH and temperature range and digests physiological proteins. The present study was carried out to evaluate the immunogenicity and protective efficacy of the MprA as a potential vaccine candidate. In BALB/c mice immunized with recombinant MprA protease (smBpF4), a significantly high IgG titer was detectable. Isotyping studies revealed that the smBpF4-specific antibodies produced were predominantly IgG1, proposing that immunization with smBpF4 triggered a Th2 immune response. Mice were immunized with smBpF4 and subsequently challenged with B. pseudomallei via the intraperitoneal route. Whilst
We evaluated the correlation of Burkholderia pseudomallei quantities in blood versus urine, sputum or pus. Correlations between bacterial counts in blood and other samples were not found. It is likely that an initial seeding event to extracellular organs is followed by independent growth of B. pseudomallei, and that bacteria in the urine were not passively filtered from the bloodstream.
The work was undertaken to expand the tools available for researching Burkholderia pseudomallei (Bp), the etiological agent of the tropical disease melioidosis. Melioidosis has the potential to pose a severe threat to public health and safety. In the United States, Bp is listed as a Tier-1 select agent by the Centers for Disease Control and Prevention (CDC), thus requiring high levels of regulation and biosafety level 3 (BSL3) facilities for experimental manipulation of live organisms. An avirulent ∆purM derivative of strain 1026b (Bp82) has proven to be a valuable tool for biosafe research as a select-agent excluded strain, but the high level of genetic diversity between Bp strains necessitates an expansion of the biosafe toolset. The ∆purM mutation was recapitulated in the Bp 576a strain, a serotype B background. An important difference between strains 1026b and 576a is the lipopolysaccharide (LPS), a major virulence factor and protective antigen. Polyclonal sera from 1026b-challenged non-human
Synonyms for Psychotria in Free Thesaurus. Antonyms for Psychotria. 1 synonym for Psychotria: genus Psychotria. What are synonyms for Psychotria?
1CI8: EstB from Burkholderia gladioli: a novel esterase with a beta-lactamase fold reveals steric factors to discriminate between esterolytic and beta-lactam cleaving activity
Caenorhabditis elegans has been used as a host for the study of bacteria that cause disease in mammals. However, a significant limitation of the model is that C elegans is not viable at 37 degrees C. We report that the gonochoristic nematode Panagrellus redivivus survives at 37 degrees C and maintains its life cycle at temperatures up to and including 31.5 degrees C. The C elegans pathogens Pseudomonas aeruginosa, Salmonella enterica, Staphylococcus aureus, but not Yersinia pseudo tuberculosis, reduced P. redivivus lifespan. Of four strains of Burkholderia multivorans tested, one reduced P. redivivus lifespan at both temperatures, one was avirulent at both temperatures and two strains reduced P. redivivus lifespan only at 37 degrees C. The mechanism by which one of these strains killed P. redivivus at 37 degrees C, but not at 25 degrees C, was investigated further. Killing required viable bacteria, did not involve bacterial invasion of tissues, is unlikely to be due to a diffusible, bacterial ...
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Development and validation of |i|Burkholderia pseudomallei|/i|-specific real-time PCR assays for clinical, environmental or forensic detection applications
Bossé, J. T., Li, Y., Angen, O., Weinert, L. A., Chaudhuri, R. R., Holden, M. T., Williamson, S. M., Maskell, D. J., Tucker, A. W., Wren, B. W., Rycroft, A. N., Langford, P. R., on behalf of the BRaDP1T consortium & Holden, M. Jul 2014 In : Journal of Clinical Microbiology. p. 2380-2385 6 p.. Research output: Contribution to journal › Article ...
Disease Ontology : 12 A primary bacterial infectious disease that results_in septicemic infection, has material basis in Burkholderia mallei, which is transmitted_by contact with tissues or body fluids of infected animals, or through mucosal surfaces such as the eyes and nose. The infection has symptom fever, has symptom chills, has symptom sweating, has symptom muscle aches, has symptom chest pain, has symptom muscle tightness, has symptom headache, has symptom mucopurulent nasal discharge, and has symptom nodular lesions in the lungs ...
Burkholderia sp. RP007 aromatic 1,2-dioxygenase beta subunit (phnY),chloroplast-type ferredoxin (phnT2), catechol 2,3-dioxygenase (phnE2), and(phnX) genes, complete cds; and 2-hydroxymuconic semialdehyde dehydrogenase(phnG2) gene, partial ...
Lineage: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Burkholderia; pseudomallei group; Burkholderia ...
Scientists may have found a way to kill Burkholderia cenocepacia, a multi-drug resistant plant pathogen that causes severe infections in people with cystic fibrosis.. The team of researchers has identified a weakness in the armour that protects the B. cenocepacia bacterium from the effects of antibiotics. They hypothesize that preventing the synthesis of a key sugar required for this armour, 4-amino-4-deoxy-L-arabinose (Ara4N), may lead to a susceptibility within the cell membrane to antibiotics. Researchers hope to discover novel molecules as antibiotic candidates which could disrupt the synthesis of Ara4N.. ...
4B6Z: High-Resolution Structure of the M14-Type Cytosolic Carboxypeptidase from Burkholderia Cenocepacia Refined Exploiting Pdb_Redo Strategies.
Melioidosis is diagnosed by isolating Burkholderia pseudomallei from blood, urine, sputum, skin lesions, or abscesses; or by detecting an antibody response to the bacteria.
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NOTE: This tree is based on information from the NCBI Taxonomy Browser, MycoBank and Index Fungorum. For the species shown in bold, the genome sequence is already publicly available (or will be soon). ...
SWISS-MODEL Repository entry for A0A1B4HVZ7 (A0A1B4HVZ7_9BURK), ATP-dependent Clp protease adapter protein ClpS. Burkholderia metallica
Gardening question - Gladioli question: My gladiolis havent flowered this year. Should i leave bulbs in or remove them? What should i do with the leaves? Still new to
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Background Genome characterization of the model PCB-degrading bacterium Burkholderia xenovorans LB400 revealed the presence of eleven central pathways for aromatic compounds degradation, among them, the homogentisate and the homoprotocatechuate pathways. However, the functionality of these central pathways in strain LB400 has not been assessed and related peripheral pathways has not been described. Methodology/Principal Findings The aims of this study were to determine the functionality of the homogentisate and homoprotocatechuate central pathways in B. xenovorans LB400 and to establish their role in 3-hydroxyphenylacetate (3-HPA) and 4-hydroxyphenylacetate (4-HPA) catabolism. Strain LB400 was able to grow using 3-HPA and 4-HPA as sole carbon source. A genomic search in LB400 suggested the presence of mhaAB and hpaBC genes clusters encoding proteins of the 3-hydroxyphenylacetate and 4-hydroxyphenylacetate peripheral pathways. LB400 cells grown with 3-HPA and 4-HPA degraded homogentisate and
Glowicz J, Crist M, Gould C, Moulton-Meissner H, Noble-Wang J, de Man TJB, Perry KA, Miller Z, Yang WC, Langille S, Ross J, Garcia B, Kim J, Epson E, Black S, Pacilli M, LiPuma JJ, Fagan R, B. cepacia Investigation Workgroup. 2018. A multistate investigation of health care-associated Burkholderia cepacia complex infections related to liquid docusate sodium contamination, January-October 2016. Am J Infect Control 46:649-655.. Brooks RB, Mitchell PK, Miller JR, Vasquez AM, Havlicek J, Lee H, Quinn M, Adams E, Baker D, Greeley R, Ross K, Daskalaki I, Walrath J, Moulton-Meissner H, Crist MB, Burkholderia cepacia Workgroup. 2018. Multistate Outbreak of Burkholderia cepacia Complex Bloodstream Infections After Exposure to Contaminated Saline Flush Syringes - United States, 2016-2017. Clin Infect Dis Off Publ Infect Dis Soc Am.. Becker SL, Berger FK, Feldner SK, Karliova I, Haber M, Mellmann A, Schäfers H-J, Gärtner B. 2018. Outbreak of Burkholderia cepacia complex infections associated with ...
At the beginning of this review it is essential to clarify the terminology that will be used to refer to the members of the Burkholderia cepacia complex and their relatives. The name B. cepacia will relate only to B. cepacia genomovar I. Strains resembling B. cepacia may belong to the B. cepacia complex, to otherBurkholderia species (for instance, Burkholderia gladioli), or to species from other genera (for instance,Ralstonia pickettii) that share some phenotypic or genotypic similarities with the B. cepacia complex. B. cepacia complex bacteria and organisms that may be confused with them will be altogether referred to as B. cepacia-like organisms. Most previous reports regarding these organisms were published before the recognition of the complicated taxonomic relationships between the different members of the B. cepacia complex; it is therefore unclear to what category the presumed B. cepacia isolates described would belong. For that reason, when such literature is cited, the name "B. cepacia" ...
Burkholderia cenocepacia ATCC ® BAA-245D-5™ Designation: Genomic DNA from Burkholderia cenocepacia strain LMG 16656 TypeStrain=False Application:
Bacteria of the Burkholderia cepacia complex (Bcc) are pathogens of humans, plants, and animals. Burkholderia cenocepacia is one of the most common Bcc species infecting cystic fibrosis (CF) patients and its carriage is associated with poor prognosis. In this study, we characterized a general O-link …
Other names: ATCC 25416, B. cepacia, Burkholderia cepacia, Burkholderia cepacia genomovar I, CCUG 12691, CCUG 13226, CFBP 2227, CIP 80.24, DSM 7288, ICMP 5796, IFO 14074, JCM 5964, NBRC 14074, NCCB 76047, NCPPB 2993, NCTC 10743, NRRL B-14810, Pseudomonas cepacia, Pseudomonas kingii, Pseudomonas multivorans, strain 717-ICPB 25, strain Ballard 717 ...
Other names: ATCC 25416, B. cepacia, Burkholderia cepacia, Burkholderia cepacia genomovar I, CCUG 12691, CCUG 13226, CFBP 2227, CIP 80.24, DSM 7288, ICMP 5796, IFO 14074, JCM 5964, NBRC 14074, NCCB 76047, NCPPB 2993, NCTC 10743, NRRL B-14810, Pseudomonas cepacia, Pseudomonas kingii, Pseudomonas multivorans, strain 717-ICPB 25, strain Ballard 717 ...
Citation. Tumapa, S., Holden, M. T., Vesaratchavest, M., Wuthiekanun, V., Limmathurotsakul, D., Chierakul, W., Feil, E. J., Currie, B. J., Day, N. P., Nierman, W. C., Peacock, S. J.. Burkholderia pseudomallei Genome Plasticity Associated With Genomic Island Variation. BMC Genomics. 2008 Apr 25; 9(1): 190.. PubMed Citation. Abstract. ABSTRACT: BACKGROUND: Burkholderia pseudomallei is a soil-dwelling saprophyte and the cause of melioidosis. Horizontal gene transfer contributes to the genetic diversity of this pathogen and may be an important determinant of virulence potential. The genome contains genomic island (GI) regions that encode a broad array of functions. Although there is some evidence for the variable distribution of genomic islands in B. pseudomallei isolates, little is known about the extent of variation between related strains or their association with disease or environmental survival. RESULTS: Five islands from B. pseudomallei strain K96243 were chosen as representatives of ...
Pulmonary infection with Burkholderia cepacia complex (BCC) in patients with CF is often associated with a more rapid decline in lung function. Because of the resistance of BCC to many antibiotics, treatment options are often limited. New therapies to improve outcomes for patients infected with BCC are needed.. However, because of the unpredictable nature of this pulmonary infection in CF, patients with BCC infection have been excluded from many CF therapeutic trials.. Recent published trials in the United States, Australia, and the United Kingdom have all demonstrated clinical benefits from prolonged administration of azithromycin in CF. In these trials, the vast majority of patients were chronically infected with Pseudomonas aeruginosa.. Patients with BCC were excluded from the US and UK trials, and only four patients with BCC infection were enrolled in the Australian trial. Thus, the effectiveness of azithromycin in CF patients infected with BCC is largely unknown and deserves further ...
Burkholderia cenocepacia is an opportunistic pathogen causing life-threatening infections in cystic fibrosis and other immunocompromised patients. The bacterium survives within macrophages by interfering with typical endocytic trafficking, resulting in delayed maturation of a B. cenocepacia-containing phagosome. We hypothesize that B. cenocepacia alters gene expression after internalization by macrophages, inducing genes involved in intracellular survival and host adaptation. Furthermore, we hypothesize that specialized bacterial secretion systems are involved in the interactions between intracellular bacteria and macrophages. In this work, we characterize later-stage infection of macrophages by B. cenocepacia, showing replication within an acidified endosomal compartment suggestive of a phagolysosome. We examine differential gene expression by intracellular B. cenocepacia using selective capture of transcribed sequences (SCOTS) with both competitive enrichment and microarray analysis. We identified 766
Burkholderia cepacia ATCC ® 17616D-5™ Designation: Genomic DNA from Burkholderia cepacia strain 249 TypeStrain=False Application:
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Neurologic melioidosis is a serious, potentially fatal form of Burkholderia pseudomallei infection. Recently, we reported that a subset of clinical isolates of B. pseudomallei from Australia have heightened virulence and potential for dissemination to the central nervous system. In this study, we demonstrate that this subset has a B. mallei-like sequence variation of the actin-based motility gene, bimA. Compared with B. pseudomallei isolates having typical bimA alleles, isolates that contain the B. mallei-like variation demonstrate increased persistence in phagocytic cells and increased virulence with rapid systemic dissemination and replication within multiple tissues, including the brain and spinal cord, in an experimental model. These findings highlight the implications of bimA variation on disease progression of B. pseudomallei infection and have considerable clinical and public health implications with respect to the degree of neurotropic threat posed to human health.
Neurologic melioidosis is a serious, potentially fatal form of Burkholderia pseudomallei infection. Recently, we reported that a subset of clinical isolates of B. pseudomallei from Australia have heightened virulence and potential for dissemination to the central nervous system. In this study, we demonstrate that this subset has a B. mallei-like sequence variation of the actin-based motility gene, bimA. Compared with B. pseudomallei isolates having typical bimA alleles, isolates that contain the B. mallei-like variation demonstrate increased persistence in phagocytic cells and increased virulence with rapid systemic dissemination and replication within multiple tissues, including the brain and spinal cord, in an experimental model. These findings highlight the implications of bimA variation on disease progression of B. pseudomallei infection and have considerable clinical and public health implications with respect to the degree of neurotropic threat posed to human health.
Abstract Melioidosis is a tropical disease of high mortality caused by the environmental bacterium, Burkholderia pseudomallei. We have collected clinical isolates from the highly endemic Northern Territory of Australia routinely since 1989, and animal and environmental B. pseudomallei isolates since 1991. Here we provide a complete record of all B. pseudomallei multilocus sequence types (STs) found in the Northern Territory to date, and distribution maps of the eight most common environmental STs. We observed surprisingly restricted geographic distributions of STs, which is contrary to previous reports suggesting widespread environmental dissemination of this bacterium. Our data suggest that B. pseudomallei from soil and water does not frequently disperse long distances following severe weather events or by migration of infected animals.
Abstract Burkholderia pseudomallei is the causative agent of melioidosis, a severe infection endemic to many tropical regions. Lipopolysaccharide (LPS) is recognized as an important virulence factor used by B. pseudomallei. Isolates of B. pseudomallei have been shown to express one of four different types of LPS (typical LPS, atypical LPS types B and B2, and rough LPS) and in vitro studies have demonstrated that LPS types may impact disease severity. The association between LPS types and clinical manifestations, however, is still unknown, in part because an effective method for LPS type identification is not available. Thus, we developed antigen capture immunoassays capable of distinguishing between the LPS types. Mice were injected with B or B2 LPS for atypical LPS-specific monoclonal antibody (mAb) isolation; only two mAbs (3A2 and 5B4) were isolated from mice immunized with B2 LPS. Immunoblot analysis and surface plasmon resonance demonstrated that 3A2 and 5B4 are reactive with both B2 and B LPS
Burkholderia pseudomallei is a Gram-negative bacterium that causes the serious human disease, melioidosis. There is no vaccine against melioidosis and it can be fatal if not treated with a specific antibiotic regimen, which typically includes the third-generation cephalosporin, ceftazidime (CAZ). There have been several resistance mechanisms described for B. pseudomallei, of which the best described are amino acid changes that alter substrate specificity in the highly conserved class A β-lactamase, PenA. In the current study, we sequenced penA from isolates sequentially derived from two melioidosis patients with wild-type (1.5 µg/mL) and, subsequently, resistant (16 or ≥256 µg/mL) CAZ phenotypes. We identified two single-nucleotide polymorphisms (SNPs) that directly increased CAZ hydrolysis. One SNP caused an amino acid substitution (C69Y) near the active site of PenA, whereas a second novel SNP was found within the penA promoter region. In both instances, the CAZ resistance phenotype ...
Ceftazidime is the antibiotic of choice for treatment of Burkholderia pseudomallei infections (melioidosis). The chromosomally encoded PenA β-lactamase possesses weak cephalosporinase activity. The wild-type penA gene confers clinically significant ceftazidime resistance only when overexpressed due to a promoter mutation, transcriptional anti-termination or by gene duplication and amplification (GDA). Here we characterize a reversible 33-kb GDA event involving wild-type penA in a ceftazidime resistant clinical isolate from Thailand. We show that duplication arises from exchanges between short (|10 base pairs, bp) chromosomal sequences, which in this example consist of 4 bp repeats flanked by 3 bp inverted repeats. GDA involving β-lactamase may be a common ceftazidime resistance mechanism in B. pseudomallei.
Review question We looked for evidence of which antibiotics are best to treat a flare up of symptoms in people with cystic fibrosis with persistent Burkholderia cepacia complex lung infection.. Background Cystic fibrosis is a common inherited condition where the lungs often become blocked with mucus. This harms the lungs defences and often results in chronic, persistent infections that cannot be cleared by antibiotics. People with cystic fibrosis often need courses of antibiotics to reduce their symptoms (for instance cough, excess mucus and breathlessness) when these flare up or worsen. Such episodes are called exacerbations, and are usually treated with intravenous antibiotics (given through a drip into a vein). One group of bacteria that can infect the lungs of people with cystic fibrosis is called the Burkholderia cepacia complex. These closely-related bacteria are found widely in the environment and do not cause infections in healthy people who do not have cystic fibrosis. They are ...
Kynurenine formamidase (KynB) forms part of the kynurenine pathway which metabolises tryptophan to anthranilate. This metabolite can be used for downstream production of 2-alkyl-4-quinolone (AQ) signalling molecules that control virulence in Pseudomonas aeruginosa. Here we investigate the role of kynB in the production of AQs and virulence-associated phenotypes of Burkholderia pseudomallei K96243, the causative agent of melioidosis. Deletion of kynB resulted in reduced AQ production, increased biofilm formation, decreased swarming and increased tolerance to ciprofloxacin. Addition of exogenous anthranilic acid restored the biofilm phenotype, but not the persister phenotype. This study suggests the kynurenine pathway is a critical source of anthranilate and signalling molecules that may regulate B. pseudomallei virulence ...
Burkholderia ambifaria strain LMG19467 histidinol-phosphate aminotransferase (hisC) gene, partial cds; imidazole glycerol phosphate dehydratase (hisB), multiple antibiotic resistance-related protein (marC), imidazole glycerol phosphate synthase glutamine amidotransferase subunit (hisH), phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (hisA), imidazole glycerol phosphate synthase subunit (hisF), phosphoribosyl-AMP cyclohydrolase (hisI), and phosphoribosyl-ATP pyrophosphohydrolase (hisE) genes, complete cds; and membrane protein gene, partial ...
Appl Environ Microbiol 68: 173-180. Wang H, Marjomaki Book, Ovod Deal, Kulomaa MS( 2002) Numic purification of part iron in Sphingobium Palace ATCC 39723. Biochem Biophys Res Commun 299: 703-709. Nakamura haben, Motoyama file, Hirono S, Yamaguchi I( 2004) Identification, 4:00PM, and Fourth agriculture of inorganic book survey. Biochim Biophys Acta 1700: 151-159. Martin-Le Garrec G, Artaud I, Capeillere-Blandin C( 2001) war and ineffective pictures of the importance body from Burkholderia cepacia interpretation AC1100. Biochim Biophys Acta 1547: 288-301. Webb BN, Ballinger JW, Kim E, Belchik SM, Lam KS, Youn B, Nissen MS, Xun LY, Kang C( 2010) Characterization of Chlorophenol 4-Monooxygenase( TftD) and NADH: capitalism Oxidoreductase( TftC) of Burkholderia cepacia AC1100. Gisi MR, Xun LY( 2003) Characterization of chlorophenol 4-monooxygenase( TftD) and NADH: download Efectos del cambio climático en las costas de Chile 2009 deterioration physiology distress( TftC) of Burkholderia cepacia AC1100. ...
A novel Gram-stain-negative, aerobic, non-spore-forming, non-motile and rod-shaped bacterial strain, DHC34T, was isolated from forest soil of Dinghushan Biosphere Reserve, Guangdong Province, China (112° 31′ E 23° 10′ N). It grew optimally on R2A medium at 28 °C, at pH 6.0-7.0 and in the presence of 0-1 % (w/v) NaCl. Strain DHC34T was closely related to Burkholderia alpina LMG 28138T (98.5 % 16S rRNA gene sequence similarity). 16S rRNA gene sequence analysis showed that strain DHC34T formed a clade with B. alpina LMG 28138T, which is next to but branched deeply with Robbsia andropogonis ICMP 2807T. The phylogenetic relationships among these three strains were also supported with the phylogram based on concatenated partial gyrB, recA and trpB gene sequences. The phylogenomic tree generated with the UBCG tool showed that strains DHC34T and R. andropogonis ICMP 2807T were in a different clade. The DNA-DNA relatedness values between strain DHC34T and B. alpina LMG 28138T and R. andropogonis
GONZALEZ-PEREZ, Enrique; YANEZ-MORALES, María de Jesús; ORTEGA-ESCOBAR, Héctor Manuel y VELAZQUEZ-MENDOZA, Juan. Comparative Analysis among Pathogenic Fungal Species that Cause Gladiolus (Gladiolus grandiflorus Hort.) Corm Rot in Mexico. Rev. mex. fitopatol [online]. 2009, vol.27, n.1, pp.45-52. ISSN 2007-8080.. An unknown disease causing wilting and death of gladiolus plants was studied in 2004, in Tlacotepec de José Manzo, in the municipality of San Salvador el Verde, located in the region of San Martín Texmelucan, Puebla, Mexico. The objective was to identify and characterize by ITS-PCR the plant pathogen(s) that cause this disease and to comparatively analyze the fungal species involved. Five species of fungi were identified from healthy and symptomatic corms taken in the field and from storage, and also from damaged basal stem and neck tissues of field plants, these were: Fusarium oxysporum f. gladioli, F. solani, Penicillium sp.; and two previously reported Acremonium strictum and ...
"Genus Burkholderia". List of Prokaryotic Names with Standing in Nomenclature. Retrieved 15 November 2016. Yabuuchi, E; Kosako, ... Y; Yano, I; Hotta, H; Nishiuchi, Y (1995). "Transfer of two Burkholderia and an Alcaligenes species To ralstonia gen. nov.: ...
Burkholderia cepacia • Campylobacter jejuni • Capnocytophaga ochracea • Corynebacterium xerosis • Enterobacter cloacae • ...
"Burkholderia caryophylli". EPPO Bulletin. 36 (1): 95-98. Jun 2006. doi:10.1111/j.1365-2338.2006.00918.x. Retrieved 2014-10-20. ... "Data Sheets on Quarantine Pests-Burkholderia caryophylli" (PDF). CABI and EPPO for the EU. Archived from the original (PDF) on ... "Scientific Opinion on the risk to plant health posed by Burkholderia caryophylli for the EU territory with the identification ... Bacterial Wilt of Carnations is a bacterial disease caused by the plant pathogen Burkholderia caryophylli. Previously, named ...
G. sporarum and Burkholderia sp. have been identified to be β-proteobacteria, a gram-negative class of bacteria, and N. ... Burkholderia sp. in R. microsporus have been found to produce rhizoxin, an inhibitor of mitosis originally thought to be ... The production of rhizoxin by Burkholderia sp. leading to the death of plant cells allows R. microsporus to gain greater access ... R. microsporus relies on its bacterial partner of the Burkholderia sp. for the pathogenic toxin. Previous efforts to control ...
Pseudomonas cepacia, a.k.a. Burkholderia cepacia. Nocardia. fungi Aspergillus species. Aspergillus has a propensity to cause ...
Burkholderia infection usually Burkholderia cepacia and other Burkholderia species Buruli ulcer Mycobacterium ulcerans ...
Brett PJ, DeShazer D, Woods DE (January 1998). "Burkholderia thailandensis sp. nov., a Burkholderia pseudomallei-like species ...
2011). "Burkholderia multivorans acts as an antagonist against the growth of Burkholderia pseudomallei in soil". Microbiology ... 2005). "Comparison of Ashdown's Medium, Burkholderia cepacia Medium, and Burkholderia pseudomallei Selective Agar for Clinical ... Burkholderia pseudomallei Center for Biosecurity Agent Fact Sheet Burkholderia pseudomallei genomes and related information at ... Burkholderia cepacia medium may be a useful alternative selective medium in nonendemic areas, where Ashdown's is not available ...
"Organism Overview: Burkholderia rhizoxinica". National Center for Biotechnology Information. Retrieved 2014-11-18. Partida- ... The resources gathered are a result of the symbiotic relationship with Burkholderia species, allowing for rhizoxin production ... This fungus contains the bacterial endosymbiont Burkholderia rhizoxinica that produces the antitumor drug rhizoxin. Certain ... Burkholderia rhizoxinica) form a symbiotic relationship. In order to kill the living cells of its host, the harbored ...
And transfer of seven species of the genus Pseudomonas homology group II to the new genus, with the type species Burkholderia ... Yabuuchi, E.; Kosako, Y.; Yano, I.; Hotta, H.; Nishiuchi, Y. (1995). "Transfer of two Burkholderia and an Alcaligenes species ... Other strains previously classified in the genus Pseudomonas are now classified in the genera Burkholderia and Ralstonia. In ... and can be used to also include previous members such as the genera Burkholderia and Ralstonia. α proteobacteria: P. ...
... is a term commonly used within the genera Burkholderia and Agrobacterium to denote strains which are phylogenetically ...
Burkholderia, Ralstonia and related organisms; Campylobacter and related bacteria; Clostridia and Clostridium-like organisms; ...
Now Burkholderia caryophylli Nelson, P.E.; Horst, R.K. "Cornell University Faculty Memorial Statement". Cornell University ...
Burkholderia calva" Van Oevelen et al. 2004 "Ca. Burkholderia crenata(♦) "Ca. Burkholderia hispidae" Lemaire et al. 2012 "Ca. ... Burkholderia kirkii" Van Oevelen et al. 2002 "Ca. Burkholderia mamillata(♦) "Ca. Burkholderia nigropunctata" Van Oevelen et al ... Burkholderia petitii" Lemaire et al. 2011 "Ca. Burkholderia rigidae" Lemaire et al. 2012 "Ca. Burkholderia schumannianae" ... Burkholderia verschuerenii(♦) "Ca. Burkholderia virens(♦) "Ca. Caedibacter acanthamoebae" Horn et al. 1999 "Ca. Caldiarchaeum ...
The bacteria are identified as Burkholderia, which is a genus that is also found in the leaves of other Rubiaceae species. ... Verstraete B, Janssens S, Smets E, Dessein S (2013). "Symbiotic beta-proteobacteria beyond legumes: Burkholderia in Rubiaceae ... associated with Burkholderia bacteria in sub-Saharan Africa". Am. J. Bot. 100 (12): 2380-7. doi:10.3732/ajb.1300303. PMID ...
2004). "Structural flexibility in the Burkholderia mallei genome". Proc. Natl. Acad. Sci. U.S.A. 101 (39): 14246-51. Bibcode: ...
Eventually, Pseudomonas aeruginosa (and sometimes Burkholderia cepacia) dominates. By 18 years of age, 80% of patients with ... Burkholderia) cepacia and multiresistant Pseudomonas aeruginosa". Neth J Med. 46 (6): 280-87. doi:10.1016/0300-2977(95)00020-N ... "The environmental risk factors associated with medical and dental equipment in the transmission of Burkholderia (Pseudomonas) ...
For Burkholderia pseudomallei, some clinical antibiotic agents that are used to treat its infection, mellioidosis, can induce ... Kang Chen; Guang Wen Sun; Kim Lee Chua; Yunn-Hwen Gan (March 2005). "Modified Virulence of Antibiotic-Induced Burkholderia ...
The bacteria are identified as Burkholderia, which is a genus that is also found in the leaves of other Rubiaceae species. The ... associated with Burkholderia bacteria in sub-Saharan Africa". American Journal of Botany. 100: 2380-2387. doi:10.3732/ajb. ... Burkholderia in Rubiaceae". PLoS ONE. 8: e55260. doi:10.1371/journal.pone.0055260. PMC 3555867 . PMID 23372845. Verstraete B, ...
The Burkholderia cepacia complex has been implicated in vertebral osteomyelitis in intravenous drug users. In general, ... Weinstein, Lenny; Knowlton, Christin A.; Smith, Miriam A. (2007-12-16). "Cervical osteomyelitis caused by Burkholderia cepacia ...
Inglis, TJ; Chiang, D; Lee, GS; Chor-Kiang, L (1998). "Potential misidentification of Burkholderia pseudomallei by API 20NE". ... Care must be taken because Burkholderia pseudomallei is commonly misidentified as C. violaceum by many common identification ... Lowe, P; Engler, C; Norton, R (2002). "Comparison of Automated and Nonautomated Systems for Identification of Burkholderia ...
Significant bacterial plant pathogens: Burkholderia Proteobacteria Xanthomonas spp. Pseudomonas spp. Pseudomonas syringae pv. ...
The bacteria are identified as Burkholderia, which is a genus that is also found in the leaves of other Rubiaceae species. The ... Verstraete B, Janssens S, Smets E, Dessein S (2013). "Symbiotic beta-proteobacteria beyond legumes: Burkholderia in Rubiaceae ... associated with Burkholderia bacteria in sub-Saharan Africa". American Journal of Botany. 100: 2380-2387. doi:10.3732/ajb. ...
"Structure of a Burkholderia pseudomallei trimeric autotransporter adhesin head". PLoS ONE. 5 (9): e12803. doi:10.1371/journal. ...
Burkholderia cepacia Proteus mirabilis and P. vulgaris Enterobacter spp. Bacteroides spp. Fusobacterium spp. Notable organisms ...
In Burkholderia pseudomallei BimA initiates actin polymerization in vitro. It is assumed that intracellular migration of this ...
ABSTRACT: BACKGROUND: Burkholderia pseudomallei is a soil-dwelling saprophyte and the cause of melioidosis. Horizontal gene ...
Melioidosis caused by the environmental gram-negative bacillus Burkholderia pseudomallei, is classically characterized by ... New insights into Burkholderia pseudomallei infection (melioidosis). International Congress on Bacteriology & Infectious ... Melioidosis caused by the environmental gram-negative bacillus Burkholderia pseudomallei, is classically characterized by ... with a special interest Burkholderia pseudomallei and Salmonella typhi infection and the role of diabetes as well as the gut ...
Burkholderia mallei, Burkholderia pseudomallei,Burkholderia gladioli, Burkholderia plantarii,Burkholderia glumae, Burkholderia ... Burkholderia ubonensis, Burkholderia caledonica, Burkholderia fungorum,Burkholderia stabilis, and Burkholderia ambifaria(1, 10 ... Burkholderia multivorans, Burkholderia glathei, Burkholderia pyrrocinia, Burkholderia thailandensis,Burkholderia graminis, ... Burkholderia vietnamiensis, Burkholderia multivorans, Burkholderia stabilis, and Burkholderia cepacia genomovars I and III. J. ...
59 BASIC BIOLOGICAL SCIENCES; Biological Science; Burkholderia; infection; RNA interference; autophagy; intracellular bacteria ... We focused on the role of the novel protein kinase C isoform, PKC-η, in Burkholderia infection and characterized PKC-η/MARCKS ... Pathogenic Burkholderia rely on host factors for efficient intracellular replication and are highly refractory to antibiotic ... To identify host genes that are required by Burkholderia spp. during infection, we performed a RNA interference (RNAi) screen ...
Among these novel natural biocides are the bioactive secondary metabolites of Burkholderia gladioli pv. agaricicola (Bga). The ... In Vitro Antifungal Activity of Burkholderia gladioli pv. agaricicola against Some Phytopathogenic Fungi. Hazem S. Elshafie 1,2 ... Keywords: Burkholderia gladioli pv. agaricicola; antifungal activity; volatile organic compounds; extracellular enzymes; ... "In Vitro Antifungal Activity of Burkholderia gladioli pv. agaricicola against Some Phytopathogenic Fungi." Int. J. Mol. Sci. 13 ...
Background and Aim Burkholderia gladioli is a rare cause of bacteraemia and sepsis in patients without predisposing factors ...
The International Burkholderia cepacia Working Group Fostering research in Burkholderia cepacia complex ... Burkholderia cepacia Workgroup. 2018. Multistate Outbreak of Burkholderia cepacia Complex Bloodstream Infections After Exposure ... An outbreak of Burkholderia stabilis colonization in a nasal ward. Int J Infect Dis IJID Off Publ Int Soc Infect Dis 33:71-74. ... Burkholderia and lung transplant. Goodlet KJ, Nailor MD, Omar A, Huang JL, LiPuma JJ, Walia R, Tokman S. 2018. Successful Lung ...
CF patients are at an increased risk of respiratory infections, including those by the environmental bacterium Burkholderia ... CF patients are at an increased risk of respiratory infections, including those by the environmental bacterium Burkholderia ... Within-host evolution of Burkholderia pseudomallei during chronic infection of seven Australasian cystic fibrosis patients. ... Within-host evolution of Burkholderia pseudomallei during chronic infection of seven Australasian cystic fibrosis patients ...
It is caused by infection with the bacterium Burkholderia mallei, usually by ingestion of contaminated food or water. Signs of ... Burkholderia mallei genomes and related information at PATRIC, a Bioinformatics Resource Center funded by NIAID ...
Glanders is caused by Burkholderia mallei, a Gram-negative, non-motile, facultative intracellular organism that is an obligate ... Glanders is caused by Burkholderia mallei, a Gram-negative, non-motile, facultative intracellular organism that is an obligate ...
Glanders is an infectious disease that is caused by the bacterium Burkholderia mallei. While people can get the disease, ...
October 2005). "Comparison of Ashdowns medium, Burkholderia cepacia medium, and Burkholderia pseudomallei selective agar for ... 2011). "Rapid identification of Burkholderia pseudomallei and Burkholderia mallei by fluorescence in situ hybridization (FISH) ... Pathema Burkholderia resource "Burkholderia pseudomallei". NCBI Taxonomy Browser. 28450. ... Burkholderia thailandensis) and arabinose-negative Burkholderia pseudomallei". Microbiology and immunology. 43 (7): 625-30. doi ...
Burkholderia cepacia [burk-hōld-er-ee-uh si-pay-shee-uh] (also called B. cepacia) is the name for a group or "complex" of ... For more information see the Notice to Readers: Manufacturers Recall of Nasal Spray Contaminated with Burkholderia cepacia ... For more information see the Notice to Readers: Nosocomial Burkholderia cepacia Infections Associated with Exposure to ...
p>An evidence describes the source of an annotation, e.g. an experiment that has been published in the scientific literature, an orthologous protein, a record from another database, etc.,/p> ,p>,a href="/manual/evidences">More…,/a>,/p> ...
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... glathei Burkholderia glumae Burkholderia grimmiae Burkholderia humi Burkholderia lata Burkholderia latens Burkholderia mallei ... Burkholderia ambifaria Burkholderia anthina Burkholderia arboris Burkholderia cenocepacia Burkholderia cepacia Burkholderia ... Burkholderia cocovenenans Burkholderia contaminans Burkholderia diffusa Burkholderia dolosa Burkholderia gladioli Burkholderia ... Burkholderia stabilis Burkholderia stagnalis Burkholderia telluris Burkholderia terrestris Burkholderia territorii Burkholderia ...
The multifarious, multireplicon Burkholderia cepacia complex.. Mahenthiralingam E1, Urban TA, Goldberg JB. ... The Burkholderia cepacia complex (Bcc) is a collection of genetically distinct but phenotypically similar bacteria that are ...
Burkholderia fungorum is a species of proteobacteria. Some of its strains can use uranium for their growth and convert U(VI) to ...
Detection of Pseudomonas (Burkholderia) cepacia using PCR.. Campbell PW 3rd1, Phillips JA 3rd, Heidecker GJ, Krishnamani MR, ...
Organism: BURKHOLDERIA MALLEI NCTC 10229 18. BURM9_1_PE1013 (BURM9_1.PE1013). Organism: BURKHOLDERIA MALLEI NCTC 10229 19. ... Organism: BURKHOLDERIA MALLEI NCTC 10229 If you have problems or comments... Back to PBIL home page ... Organism: BURKHOLDERIA MALLEI NCTC 10229 2. BURM9_1_PE10 RecName: Full=UvrABC system protein B; (BURM9_1.PE10). Keywords: ... Organism: BURKHOLDERIA MALLEI NCTC 10229 16. BURM9_1_PE1011 SubName: Full=Rhs element Vgr protein; (BURM9_1.PE1011). Keywords: ...
Burkholderia comprises more than 60 species of environmental, clinical, and agro-biotechnological relevance. Previous ... nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica ... Transfer of Pseudomonas plantarii and Pseudomonas glumae to Burkholderia as Burkholderia spp. and description of Burkholderia ... Burkholderia nifH Gene Acetylene Reduction Activity Melioidosis Burkholderia Species These keywords were added by machine and ...
... Taksaon Duangurai,1,2 Nitaya Indrawattana,1 and ... Burkholderia pseudomallei is a Gram-negative bacterium that causes melioidosis, which can be fatal in humans. Melioidosis is ...
Genomic DNA from Burkholderia cenocepacia strain LMG 16656 TypeStrain=False Application: ... Burkholderia cepacia (Palleroni and Holmes) Yabuuchi et al. Strain Designations Genomic DNA from Burkholderia cenocepacia ... Burkholderia cenocepacia (ATCC® BAA-245D-5™) Strain Designations: Genomic DNA from Burkholderia cenocepacia strain LMG 16656 [ ... Burkholderia cenocepacia ATCC® BAA-245D-5™ dried Total DNA: At least 5 µg in 1X TE buffer ...
Retrieved from "https://species.wikimedia.org/w/index.php?title=Burkholderia_virus_Bcepmigl&oldid=6015206" ...
NAME: Burkholderia (Pseudomonas) mallei SYNONYM OR CROSS REFERENCE: Glanders, Malleomyces mallei, Farcy, Malleus; formerly ...
  • Other glucose nonfermenters, like Stenotrophomonas maltophilia , Alcaligenes xylosoxidans , R. pickettii , and Burkholderia gladioli , can frequently be found as well, but their role in the decline of pulmonary function is unclear ( 14 , 19 , 30 ). (asm.org)
  • As a result, identification of host kinases that are targeted by Burkholderia during infection provides valuable molecular insights in understanding Burkholderia pathogenesis, and ultimately, in designing effective host-targeted therapies against infectious disease caused by intracellular pathogens. (osti.gov)
  • To identify host genes that are required by Burkholderia spp. (osti.gov)
  • We focused on the role of the novel protein kinase C isoform, PKC-η, in Burkholderia infection and characterized PKC-η/MARCKS signaling as a key event that promotes the survival of unopsonized B. thailandensis CDC2721121 within host cells. (osti.gov)
  • He investigates the role and function of pathogen-recognition-receptors and innate immunity in sepsis, with a special interest Burkholderia pseudomallei and Salmonella typhi infection and the role of diabetes as well as the gut microbiota during sepsis. (omicsonline.org)
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