A bacterial vaccine for the prevention of brucellosis in man and animal. Brucella abortus vaccine is used for the immunization of cattle, sheep, and goats.
A genus of gram-negative, aerobic bacteria that causes BRUCELLOSIS. Its cells are nonmotile coccobacilli and are animal parasites and pathogens. The bacterium is transmissible to humans through contact with infected dairy products or tissue.
Infection caused by bacteria of the genus BRUCELLA mainly involving the MONONUCLEAR PHAGOCYTE SYSTEM. This condition is characterized by fever, weakness, malaise, and weight loss.
A species of the genus BRUCELLA whose natural hosts are cattle and other bovidae. Abortion and placentitis are frequently produced in the pregnant animal. Other mammals, including humans, may be infected.
A species of the genus BRUCELLA whose natural hosts are sheep and goats. Other mammals, including humans, may be infected. In general, these organisms tend to be more virulent for laboratory animals than BRUCELLA ABORTUS and may cause fatal infections.
Suspensions of killed or attenuated microorganisms (bacteria, viruses, fungi, protozoa), antigenic proteins, synthetic constructs, or other bio-molecular derivatives, administered for the prevention, amelioration, or treatment of infectious and other diseases.
Vaccines in which the infectious microbial nucleic acid components have been destroyed by chemical or physical treatment (e.g., formalin, beta-propiolactone, gamma radiation) without affecting the antigenicity or immunogenicity of the viral coat or bacterial outer membrane proteins.
Suspensions of attenuated or killed viruses administered for the prevention or treatment of infectious viral disease.
A species of the genus BRUCELLA which are pathogenic to SHEEP.
Suspensions of attenuated or killed bacteria administered for the prevention or treatment of infectious bacterial disease.
Recombinant DNA vectors encoding antigens administered for the prevention or treatment of disease. The host cells take up the DNA, express the antigen, and present it to the immune system in a manner similar to that which would occur during natural infection. This induces humoral and cellular immune responses against the encoded antigens. The vector is called naked DNA because there is no need for complex formulations or delivery agents; the plasmid is injected in saline or other buffers.
Small synthetic peptides that mimic surface antigens of pathogens and are immunogenic, or vaccines manufactured with the aid of recombinant DNA techniques. The latter vaccines may also be whole viruses whose nucleic acids have been modified.
Two or more vaccines in a single dosage form.
A species of gram-negative bacteria infecting DOGS, the natural hosts, and causing canine BRUCELLOSIS. It can also cause a mild infection in humans.
Vaccines or candidate vaccines containing inactivated HIV or some of its component antigens and designed to prevent or treat AIDS. Some vaccines containing antigens are recombinantly produced.
A disease of cattle caused by bacteria of the genus BRUCELLA leading to abortion in late pregnancy. BRUCELLA ABORTUS is the primary infective agent.
Vaccines consisting of one or more antigens that stimulate a strong immune response. They are purified from microorganisms or produced by recombinant DNA techniques, or they can be chemically synthesized peptides.
Semisynthetic vaccines consisting of polysaccharide antigens from microorganisms attached to protein carrier molecules. The carrier protein is recognized by macrophages and T-cells thus enhancing immunity. Conjugate vaccines induce antibody formation in people not responsive to polysaccharide alone, induce higher levels of antibody, and show a booster response on repeated injection.
Administration of vaccines to stimulate the host's immune response. This includes any preparation intended for active immunological prophylaxis.
Vaccines made from antigens arising from any of the four strains of Plasmodium which cause malaria in humans, or from P. berghei which causes malaria in rodents.
Vaccines or candidate vaccines used to prevent PAPILLOMAVIRUS INFECTIONS. Human vaccines are intended to reduce the incidence of UTERINE CERVICAL NEOPLASMS, so they are sometimes considered a type of CANCER VACCINES. They are often composed of CAPSID PROTEINS, especially L1 protein, from various types of ALPHAPAPILLOMAVIRUS.
The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.
The genetic complement of a BACTERIA as represented in its DNA.
A species of bacteria that causes ANTHRAX in humans and animals.
Vaccines or candidate vaccines used to prevent ANTHRAX.
An acute infection caused by the spore-forming bacteria BACILLUS ANTHRACIS. It commonly affects hoofed animals such as sheep and goats. Infection in humans often involves the skin (cutaneous anthrax), the lungs (inhalation anthrax), or the gastrointestinal tract. Anthrax is not contagious and can be treated with antibiotics.
Substances elaborated by bacteria that have antigenic activity.
An oxidoreductase that catalyzes the reaction between superoxide anions and hydrogen to yield molecular oxygen and hydrogen peroxide. The enzyme protects the cell against dangerous levels of superoxide. EC 1.15.1.1.
A genus of the family POXVIRIDAE, subfamily CHORDOPOXVIRINAE, consisting of ether-sensitive viruses of leporids and squirrels. They commonly cause tumors and are usually transmitted mechanically by arthropods. MYXOMA VIRUS is the type species.
A serotype of Salmonella enterica that is a frequent agent of Salmonella gastroenteritis in humans. It also causes PARATYPHOID FEVER.

Immunity to Brucella in mice vaccinated with a fraction (F8) or a killed vaccine (H38) with or without adjuvant. Level and duration of immunity in relation to dose of vaccine, recall injection and age of mice. (1/138)

Immunity to Brucella in the mouse, assessed by bacterial spleen counts 15 days after intraperitoneal inoculation of a standard challenge of B. abortus 544, has been studied with two vaccines, one experimental, composed of a fraction of the bacterial cell-wall (F8) extracted from B. abortus 99, the other of killed whole bacteria, B. melitensis 53 H38, taken as reference (H38). The level of primary immunity depended on the dose of vaccine, the presence of oil adjuvant and the age of the mouse. The presence of adjuvant enabled the immunization to F8 to continue beyond the first month, to reach its maximum around the fourth month, and to remain stable for at least 7 months. A booster injection 3 or 6 months after the primary vaccination reinforced existing immunity but did not increase it beyond a certain level. The effect of the recall injection was clearly demonstrated with low doses which gave a lower level of primary immunity.  (+info)

Antibody response to antigens distinct from smooth lipopolysaccharide complex in Brucella infection. (2/138)

The smooth lipopolysaccharide complex of the outer surface of smooth Brucella abortus cells is believed to be the antigenic component involved in serological tests routinely used for the diagnosis of brucellosis. Sera from cattle vaccinated or infected with B. abortus generally contain antibody directed toward the smooth lipopolysaccharide complex. The brucella organism contains a large number of other antigenically distinct components. The biological significance of some of these antigens has been demonstrated by showing that sera from infected cattle have precipitins to these components. These sera revealed up to seven distinct lines in immunoelectrophoresis with a protein-rich antigen mixture prepared from rough strain B. abortus 45/20, whereas sera from strain 19-vaccinated cattle did not reveal these lines at 4 or more months after vaccination. Monospecific antisera were prepared against six antigens in this mixture, and the purification of two of them by antibody affinity chromatography is described.  (+info)

Protection of mice against brucellosis by vaccination with Brucella melitensis WR201(16MDeltapurEK). (3/138)

Human brucellosis can be acquired from infected animal tissues by ingestion, inhalation, or contamination of the conjunctiva or traumatized skin by infected animal products. A vaccine to protect humans from occupational exposure or from zoonotic infection in areas where the disease is endemic would reduce an important cause of morbidity worldwide. Vaccines currently used in animals are unsuitable for human use. We tested a live, attenuated, purine-auxotrophic mutant strain of Brucella melitensis, WR201, for its ability to elicit cellular and humoral immune responses and to protect mice against intranasal challenge with B. melitensis 16M. Mice inoculated intraperitoneally with WR201 made serum antibody to lipopolysaccharide and non-O-polysaccharide antigens. Splenocytes from immunized animals released interleukin-2 (IL-2), gamma interferon, and IL-10 when cultured with Brucella antigens. Immunization led to protection from disseminated infection but had only a slight effect on clearance of the challenge inoculum from the lungs. These studies suggest that WR201 should be further investigated as a vaccine to prevent human brucellosis.  (+info)

Complement fixation test to assess humoral immunity in cattle and sheep vaccinated with Brucella abortus RB51. (4/138)

The live attenuated Brucella abortus strain RB51 is a rifampin-resistant, lipopolysaccharide (LPS) O-chain-deficient mutant of virulent B. abortus 2308. The reduced O-chain content in RB51 prevents this bacterium from inducing antibodies detectable by the conventional serologic tests for bovine brucellosis diagnosis that mainly identify antibodies to LPS. The absence of available serologic tests for RB51 also complicates the diagnosis of possible RB51 infections in humans exposed to this strain. The purpose of this study was to evaluate the suitability of a complement fixation (CF) test performed with the rough strain B. abortus RB51, previously deprived of anticomplementary activity, in detecting anti-B. abortus RB51 antibodies in cattle and sheep experimentally vaccinated with this strain. The results of this study showed that a CF test with RB51 as the antigen is able to specifically detect antibodies following RB51 vaccination in cattle and sheep. In addition, this method could be a useful tool for detecting B. abortus RB51 infection in humans.  (+info)

Overexpression of protective antigen as a novel approach to enhance vaccine efficacy of Brucella abortus strain RB51. (5/138)

Brucella abortus strain RB51 is an attenuated rough strain that is currently being used as the official live vaccine for bovine brucellosis in the United States and several other countries. We reasoned that overexpression of a protective antigen(s) of B. abortus in strain RB51 should enhance its vaccine efficacy. To test this hypothesis, we overexpressed Cu/Zn superoxide dismutase (SOD) protein of B. abortus in strain RB51. This was accomplished by transforming strain RB51 with a broad-host-range plasmid, pBBR1MCS, containing the sodC gene along with its promoter. Strain RB51 overexpressing SOD (RB51SOD) was tested in BALB/c mice for its ability to protect against challenge infection with virulent strain 2308. Mice vaccinated with RB51SOD, but not RB51, developed antibodies and cell-mediated immune responses to Cu/Zn SOD. Strain RB51SOD vaccinated mice developed significantly (P < 0.05) more resistance to challenge than those vaccinated with strain RB51 alone. The presence of the plasmid alone in strain RB51 did not alter its vaccine efficacy. Also, overexpression of SOD did not alter the attenuation characteristic of strain RB51.  (+info)

Brucella abortus strain RB51 as a vector for heterologous protein expression and induction of specific Th1 type immune responses. (6/138)

Brucella abortus strain RB51 is a stable, rough, attenuated mutant widely used as a live vaccine for bovine brucellosis. Our ultimate goal is to develop strain RB51 as a preferential vector for the delivery of protective antigens of other intracellular pathogens to which the induction of a strong Th1 type of immune response is needed for effective protection. As a first step in that direction, we studied the expression of a foreign reporter protein, beta-galactosidase of Escherichia coli, and the 65-kDa heat shock protein (HSP65) of Mycobacterium bovis in strain RB51. We cloned the promoter sequences of Brucella sodC and groE genes in pBBR1MCS to generate plasmids pBBSODpro and pBBgroE, respectively. The genes for beta-galactosidase (lacZ) and HSP65 were cloned in these plasmids and used to transform strain RB51. An enzyme assay in the recombinant RB51 strains indicated that the level of beta-galactosidase expression is higher under the groE promoter than under the sodC promoter. In strain RB51 containing pBBgroE/lacZ, but not pBBSODpro/lacZ, increased levels of beta-galactosidase expression were observed after subjecting the bacteria to heat shock or following internalization into macrophage-like J774A.1 cells. Mice vaccinated with either of the beta-galactosidase-expressing recombinant RB51 strains developed specific antibodies of predominantly the immunoglobulin G2a (IgG2a) isotype, and in vitro stimulation of their splenocytes with beta-galactosidase induced the secretion of gamma interferon (IFN-gamma), but not interleukin-4 (IL-4). A Th1 type of immune response to HSP65, as indicated by the presence of specific serum IgG2a, but not IgG1, antibodies, and IFN-gamma, but not IL-4, secretion by the specific-antigen-stimulated splenocytes, was also detected in mice vaccinated with strain RB51 containing pBBgroE/hsp65. Studies with mice indicated that expression of beta-galactosidase or HSP65 did not alter either the attenuation characteristics of strain RB51 or its vaccine efficacy against B. abortus 2308 challenge.  (+info)

Structural, functional and immunological studies on a polymeric bacterial protein. (7/138)

The characterization of proteins from Brucella spp, the causative agent of brucellosis, has been the subject of intensive research. We have described an 18-kDa cytoplasmic protein of Brucella abortus and shown the potential usefulness of this protein as an antigen for the serologic diagnosis of brucellosis. The amino acid sequence of the protein showed a low but significant homology with that of lumazine synthases. Lumazine is an intermediate product in bacterial riboflavin biosynthesis. The recombinant form of the 18-kDa protein (expressed in E. coli) folds like the native Brucella protein and has lumazine-synthase enzymatic activity. Three-dimensional analysis by X-ray crystallography of the homolog Bacillus subtilis lumazine synthase has revealed that the enzyme forms an icosahedral capsid. Recombinant lumazine synthase from B. abortus was crystallized, diffracted X rays to 2.7-A resolution at room temperature, and the structure successfully solved by molecular replacement procedures. The macromolecular assembly of the enzyme differs from that of the enzyme from B. subtilis. The Brucella enzyme remains pentameric (90 kDa) in its crystallographic form. Nonetheless, the active sites of the two enzymes are virtually identical at the structural level, indicating that inhibitors of these enzymes could be viable pharmaceuticals across a broad species range. We describe the structural reasons for the differences in their quaternary arrangement and also discuss the potential use of this protein as a target for the development of acellular vaccines.  (+info)

Validation of the abbreviated Brucella AMOS PCR as a rapid screening method for differentiation of Brucella abortus field strain isolates and the vaccine strains, 19 and RB51. (8/138)

The Brucella AMOS PCR assay was previously developed to identify and differentiate specific Brucella species. In this study, an abbreviated Brucella AMOS PCR test was evaluated to determine its accuracy in differentiating Brucella abortus into three categories: field strains, vaccine strain 19 (S19), and vaccine strain RB51/parent strain 2308 (S2308). Two hundred thirty-one isolates were identified and tested by the conventional biochemical tests and Brucella AMOS PCR. This included 120 isolates identified as B. abortus S19, 9 identified as B. abortus strain RB51, 57 identified as B. abortus biovar 1, 15 identified as B. abortus bv. 2, 1 identified as B. abortus bv. 2 (M antigen dominant), 7 identified as B. abortus bv. 4, and 22 identified as B. abortus S2308 and isolated from experimentally infected cattle. The Brucella AMOS PCR correctly identified each isolate as RB51/S2308, S19, or a field strain of Brucella.  (+info)

Brucella abortus is a facultative intracellular bacterial pathogen, which causes abortion in cattle and undulant fever in human. B. abortus strain RB51 (Strain RB51) is the official vaccine for bovine brucellosis in the USA. B. abortus strain RB51 can be used as a vector for the over-expression of its own (homologous) as well as heterologous protective antigens. The immune system can detect these heterologous antigens and produce a response. Expressing a protein in different bacterial compartments has been shown to affect its accessibility to the immune system and the way the antigen is processed by antigen presenting cells. In order to determine if the immune response is affected by the localization of the antigen, green fluorescent protein (GFP) was expressed at three different locations in B. abortus strain RB51, outer-membrane (OM), periplasmic space (PS) and in the cytoplasmic region (CR) of B. abortus strain RB51. This localization was obtained by transforming strain RB51 with plasmids ...
Background: Lipopolysaccharides (LPSs) and several antigenic proteins of Brucella have been considered for preparation of diagnostic reagents and subunit vaccines. The objective of this study was to identify and compare immunogens of B. abortus S19 which induce humoral immune responses in human, goat and rabbit. Material and Methods: The bacterial whole cell extract was prepared in extraction buffer and resolved using two-dimensional gel electrophoresis (2-DE). The resolved antigens were reacted against human, goat and rabbit sera using western blotting. Results: At least 19, 14 and 16 immunogenic proteins were recognized in western blotting with human, goat and rabbit sera, respectively. The most abundant proteins of the bacterium with immunogenic properties in goat and rabbit but not in human, were a group of 5-6 proteins with molecular masses of 32-34 KDa and isoelectric point (pI) ranging from 4.5 to 5.7. In contrast, a group of 5 proteins with molecular weight of 45 KDa and pI in the range of 4.5
The goal of our proposed research is to develop a Brucella vaccine that is safe for human use. Our approach has explored the use of live attenuated vaccines, si...
Telecommunications firms have increasingly turned to fiber optics technology, which uses glass or plastic fibers to transmit data more quickly than traditional
Brucella abortus is a facultative intracellular bacterial pathogen, which causes abortion in cattle and undulant fever in human. B. abortus strain RB51 (Strain RB51) is the official vaccine for bovine brucellosis in the USA. B. abortus strain RB51 can be used as a vector for the over-expression of its own (homologous) as well as heterologous protective antigens. The immune system can detect these heterologous antigens and produce a response. Expressing a protein in different bacterial compartments has been shown to affect its accessibility to the immune system and the way the antigen is processed by antigen presenting cells. In order to determine if the immune response is affected by the localization of the antigen, green fluorescent protein (GFP) was expressed at three different locations in B. abortus strain RB51, outer-membrane (OM), periplasmic space (PS) and in the cytoplasmic region (CR) of B. abortus strain RB51. This localization was obtained by transforming strain RB51 with plasmids ...
Abstract. We report the draft genome sequences of two Brucella abortus strains LMN1 and LMN2 isolated from cattle and pig. The LMN1 and LMN2 have the genome size of 3,395,952 bp and 3,334,792 bp, respectively. In addition to the conserved genes of Brucella, few novel regions showing similarity to the phages were identified in both strains.. Keywords: genome sequence, Brucella abortus ...
Brucellosis is a disease that can be prevented through vaccination. Yet, the effectiveness of the vaccination to fight this disease is considered weak. Fortunately, attempts to modify brucellosis vaccine is still keep going. Some brucellosis vaccines have been found and developed in the past time such as the vaccine B.abortus strain 19-BA and 104M which was made from weakened microbes which had been widely used in Uni Soviet and China. The other brucellosis vaccine that were used in the past were the phenolinsoluble peptidoglycan vaccine which was made in France and polysaccharide-protein vaccine which was used in Russia. This research attempted to see the determinant of antigenic Outer Membrane Protein (OM) 36 kDa Brucella abortus local isolation which has immunogenic character to be developed as an advanced brucellosis vaccine. The method used in this research was the Omp2 gene of Brucella abortus of local isolate employed the PCR technique. The result of the PCR was then sequenced to analyze ...
Converts alpha-N-acetylneuranimic acid (Neu5Ac) to the beta-anomer, accelerating the equilibrium between the alpha- and beta-anomers. Probably facilitates sialidase-negative bacteria to compete sucessfully for limited amounts of extracellular Neu5Ac, which is likely taken up in the beta-anomer. In addition, the rapid removal of sialic acid from solution might be advantageous to the bacterium to damp down host responses.
Ubiquinone (UQ), also called coenzyme Q, and plastoquinone (PQ) are electron carriers in oxidative phosphorylation and photosynthesis, respectively. The quinoid nucleus of ubiquinone is derived from the shikimate pathway; 4-hydroxybenzoate is directly formed from chorismate in bacteria, while it can be formed from either chorismate or tyrosine in yeast. The following biosynthesis of terpenoid moiety involves reactions of prenylation, decarboxylation, and three hydroxylations alternating with three methylations. The order of these reactions are somewhat different between bacteria and yeast. Phylloquinone (vitamin K1), menaquinone (vitamin K2), and tocopherol (vitamin E) are fat-soluble vitamins. Phylloquinone is a compound present in all photosynthetic plants serving as a cofactor for photosystem I-mediated electron transport. Menaquinone is an obligatory component of the electron-transfer pathway in bacteria ...
Presented by Steven Olsen at a workshop on an integrated approach to controlling brucellosis in Africa, Addis Ababa, Ethiopia, 29-31 January 2013.
IMMUNOGENIC PROPERTIES OF SOLUBLE ANTIGENS OR WHOLE CELLS OF BRUCELLA ABORTUS STRAIN 45/20 ASSOCIATED WITH IMMUNOADJUVANTS. I: SOLUBLE ANTIGENSWOODARD LF; TOONE NM; MCLAUGHLIN CA et al.1980; CAN. J. COMP. MED.; ISSN 0008-4050; CAN; DA. 1980; VOL. 44; NO 4; PP. 453-455; ABS. FRE; BIBL. 9 REF.Article ...
The roles of humoral and cell mediated immune responses in murine brucellosis were investigated in this study.B. abortus strain 19, the current vaccine strain, is known to induce an antibody as well as cell mediated immune responses, both of which protect mice against smooth strain 2308. B. abortus rough strain RB51 does not induce an o-side chain specific antibody response and yet protects mice against smooth strain 2308. Passive transfer experiments using serum and nylon wool enriched T cells obtained from micevaccinated with strain 19 and strain RB51 were carried out. Immune senum from strain 19 vaccinated mice protected against challenge with strain 2308 but not strain RB51. Nylon wool enriched T cells from strain 19 vaccinated mice protected recipient mice against challenge with both strains RB51 or 2308. Serum obtained from RB51 vaccinated mice did not protect recipient mice against challenge with either strain RB51 or strain 2308. Nylon wool enriched T cells from the same vaccinated mice, ...
Spleen cells from mice infected with the rough Brucella melitensis strain B115 were fused with NSO myeloma cells. Hybridoma supernatants were screened in ELISA with cell walls (CW), sonicated cell extracts (CE) and rough lipopolysaccharide (R-LPS) of B. melitensis strain B115 and whole B. melitensis B115 cells. Surprisingly, 22 monoclonal antibodies (mAbs) reacting in ELISA with both CW and CE but not with R-LPS and bacterial cells were shown by immunoblot analysis and ELISA to react with smooth lipopolysaccharide (S-LPS). These mAbs also reacted in ELISA with O polysaccharides (OPS) from the smooth Brucella abortus strain 99 and the smooth B. melitensis strain 16M and thus recognize epitopes present on the O-chain. Proteinase K LPS preparations from B. melitensis B115 analysed by immunoblotting with one mAb (12G12) recognizing S-LPS of both A and M specificity displayed the typical S-LPS high-molecular-mass ladder pattern but no S-LPS was detected in the phenol/water/chloroform/light petroleum LPS
The Brucella abortus strain S19, a spontaneously attenuated strain, has been used as a vaccine strain in vaccination of cattle against brucellosis for six decades. Despite many studies, the physiological and molecular mechanisms causing the attenuation are not known. We have applied pyrosequencing technology together with conventional sequencing to rapidly and comprehensively determine the complete genome sequence of the attenuated Brucella abortus vaccine strain S19. The main goal of this study is to identify candidate virulence genes by systematic comparative analysis of the attenuated strain with the published genome sequences of two virulent and closely related strains of B. abortus, 9-941 and 2308. The two S19 chromosomes are 2,122,487 and 1,161,449 bp in length. A total of 3062 genes were identified and annotated. Pairwise and reciprocal genome comparisons resulted in a total of 263 genes that were non-identical between the S19 genome and any of the two virulent strains. Amongst these, 45 genes
Fingerprint Dive into the research topics of Replication of Brucella abortus and Brucella melitensis in fibroblasts does not require Atg5-dependent macroautophagy. Together they form a unique fingerprint. ...
Rabbit polyclonal antibody raised against full length recombinant Brucella suis vjbR. Recombinant protein corresponding to full length Brucella suis vjbR. (PAB16064) - Products - Abnova
PHE1 is a htrA cycL double gene deletion mutant of virulent Brucella abortus strain 2308 (S2308) which has previously been evaluated in the murine and caprine models of bovine brucellosis. This report describes the results of studies conducted with this mutant in the natural bovine host. Six sexually mature, non-gravid heifers were inoculated via the conjunctival sac with 1x1010 colony forming units (CFU) of either the parental S2308 or the htrA cycL gene deletion mutant, PHE1. At 4, 7 and 11 days post-inoculation, PHE1 was found to colonize the bovine host at lower levels than S2308. In a second experiment, eight heifers in mid-gestation were infected with 1x107CFU of either strain via the conjunctival sac. The virulent S2308 caused abortions or weak calves in 4/4 cows, while all four cows infected with PHE1 had healthy calves. Furthermore, PHE1 exhibited decreased resistance to killing by cultured bovine neutrophils and macrophages compared to the parental strain. These studies demonstrate that the B.
Pathogens infecting mammalian cells have developed various strategies to suppress and evade their hosts defensive mechanisms. In this line, the intracellular bacteria that are able to survive and propagate within their host cells must have developed strategies to avert their hosts killing attitude. Studying the interface of host-pathogen confrontation can provide valuable information for defining therapeutic approaches. Brucellosis, caused by the Brucella strains, is a zoonotic bacterial disease that affects thousands of humansand animals around the world inflicting discomfort and huge economic losses. Similar to many other intracellular dwelling bacteria, infections caused by Brucella are difficult to treat, and hence any attempt at identifying new and common therapeutic targets would prove beneficial for the purpose of curing infections caused by the intracellular bacteria. In THP-1 macrophage infected with Brucella melitensis we studied the expression levels of four hosts genes, i.e. EMP2, ST8SIA4
Brucella abortus is a Gram-negative, facultative intracellular bacterium that causes brucellosis, a worldwide zoonotic disease leading to undulant fever in humans and abortion in cattle. The immune response against this bacterium relies on the recognition of microbial pathogen-associated molecular patterns, such as lipoproteins, lipopolysaccharides and DNA; however, the immunostimulatory potential of B. abortus RNA remains to be elucidated. Here, we show that dendritic cells (DCs) produce significant amounts of IL-12, IL-6 and IP-10/CXCL10, when stimulated with purified B. abortus RNA. IL-12 secretion by DCs stimulated with RNA depends on TLR7 while IL-6 depends on TLR7 and partially on TLR3. Further, only TLR7 plays a role in IL-12 production induced by B. abortus infection. Moreover, cytokine production in DCs infected with B. abortus or stimulated with bacterial RNA was reduced upon pretreatment with MAPK/NF-κB inhibitors. By confocal microscopy, we demonstrated that TLR7 is colocalized with B.
La mayoría de las infecciones bacterianas inducen la activación de los neutrófilos polimorfonucleares (PMN), mejoran su función microbicida y promueven la supervivencia de estos leucocitos durante períodos prolongados. Brucella abortus es un patógeno sigiloso que evade la inmunidad innata, apenas activa los PMN y resiste los mecanismos de destrucción de estos fagocitos. Los intrigantes signos clínicos que se observan durante la brucelosis son el bajo número de PMN infectados por Brucella en los órganos diana y la neutropenia en una proporción de los pacientes; características que merecen una mayor atención. Aquí demostramos que B.abortus mata prematuramente a los PMN humanos de forma dosis dependiente y específica de la célula. La muerte de los PMNs es concomitante con la liberación de lipopolisacáridos intracelulares de Brucella (Br-LPS) en vacuolas. Esta molécula y su lípido A reproducen la muerte celular prematura de los PMN, un fenómeno asociado a la baja producción de ...
We report a case of Brucella abortus-induced brucellosis that displayed high hepatic transaminases similar to that in acute hepatitis, fourth month relapsed following doxycycline-streptomycin treatment, and developed doxycycline-related hepatotoxicity in second treatment. Brucella hepatitis may occur with various Brucella spp. and may relapse even following ...
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Brucella Series Symptom Relief kit is for the temporary relief of symptoms related to Brucella infection including fever, sweats, malaise, headache, back pain, neck pain, malodorous sweat, peculiar taste in mouth and gut inflammation.. A kit contains ten vials, each with a -different safe and -effective dilution of the actual deactivated virus or bacteria the kit is intended to address. The vials are taken in series, first increasing in potency for the first 30 days of the protocol, then decreasing in potency for the second 30 days to provide a full 60 days of symptom relief.. The basic 60-Day Series Protocol requires two ten-vial kits. Each vial is taken all at once, with the contents held under the tongue for 30 seconds. The first kit is taken over a 30-day period, one vial every three days, in ascending order (from #1 to #10). The second kit is then taken over 30 days (one vial every three days) in descending order (from vial #10 to vial #1).. This ascending and descending sequence provides ...
Brucella BvrR protein: BvrR/BvrS represent the two-component system involved in the regulation of Brucella virulence; amino acid sequence in first source
FUN_05: Use the SSGCID solved structure of Brucella melitensis methionine-tRNA-synthetase (MetRS) to find inhibitors for potential drug development for brucellosis.
Die Abortus Bang Ringprobe (ABR Test) ist eine serologische Untersuchung, die zum Nachweis von Brucellen in Milch genutzt wird. Hierbei werden die Antikörper (IgM, IgG, IgA) mit einem Hämatoxylin gekoppelten Antigen sichtbar demacht. Es handelt…
Brucella abortus induces an inflammatory response that stimulates the endocrine system resulting in the secretion of cortisol and dehydroepiandrosterone (DHEA). Osteoarticular brucellosis is the most common presentation of the active disease in humans and we have previously demonstrated that B. abortus infection inhibits osteoblast function. We aimed to evaluate the role of cortisol and DHEA on osteoblast during B. abortus infection. B. abortus infection induces apoptosis and inhibits osteoblast function. DHEA treatment reversed the effect of B. abortus infection on osteoblast by increasing their proliferation, inhibiting osteoblast apoptosis and reversing the inhibitory effect of B. abortus on osteoblast differentiation and function. In contrast, cortisol increased the effect of B. abortus infection. Cortisol regulates target genes by binding to the glucocorticoid receptor (GR). B. abortus infection inhibited GR-α expression. Cells responses to cortisol not only depend on GR expression but also on its
Brucella spp. are the etiological agents of brucellosis, a zoonotic infectious disease that causes abortion in animals and chronic debilitating illness in humans. Natural Brucella infections occur primarily through an incompletely defined mechanism of adhesion to and penetration of mucosal epithelium. In this study, we characterized changes in genome-wide transcript abundance of the most and the least invasive growth phases of B. melitensis cultures to HeLa cells, as a preliminary approach for identifying candidate pathogen genes involved in invasion of epithelial cells. B. melitensis at the late logarithmic phase of growth are more invasive to HeLa cells than mid-logarithmic or stationary growth phases. Microarray analysis of B. melitensis gene expression identified 414 up- and 40 down-regulated genes in late-log growth phase (the most invasive culture) compared to the stationary growth phase (the least invasive culture). As expected, the majority of up-regulated genes in late-log phase cultures were
Mapping the restriction fragments of the Brucella melitensis 16M genome with a new restriction endonuclease, PacI, which cut the DNA into only eight fragments, indicated that this species contains two unique and independent replicons of about 2,100 and 1,150 kb. Pulsed-field gel electrophoresis of intact DNA revealed two bands migrating the expected distances. These replicons were identified as two unique and independent chromosomes by the presence of rRNA operons and genes for heat shock proteins mapping to separate replicons. ...
The foodproof Brucella Detection Kit detects the genus Brucella and additionally identifies the two most common food-associated disease species Brucella abortus and Brucella melitensis. The rod-shaped, Gram-negative, aerobic bacteria are found in cows, sheep and pigs. The bacteria can be transmitted to humans through raw milk or meat causing brucellosis, also called Malta Fever.
Dendogram of global Brucella abortus biovar 1 genotypes. Dendogram based on MLVA-16 genotyping showing the relationship of 49 B. abortus biovar 1 strains origin
Brucella causes a chronic and debilitating infection that leads to great economic losses and a public health burden. In this study, we demonstrated the brucellacidal effect of heat shock mediated by the induction of pro-inflammatory cytokines, reactive oxygen species (ROS) accumulation and apoptosis in murine macrophages and in mice. RAW264.7 cells were incubated at 43 °C, and BALB/c mice were subjected to whole body hyperthermia. The data showed a reduction in bacterial survival in the mice after daily heat exposure. This was accompanied by increased levels of cytokines TNF, IL-6, IL-1β and IFN-γ in the sera of the mice. Gene expression of NF-κB and inducible nitric oxide production were also induced in the mouse splenic cells. In parallel with the bacterial reduction in the mouse model, an increased bactericidal effect was observed in RAW264.7 cells after exposure to heat stress. In addition, the heat stress increased both the nuclear translocation of NF-κB and the expression of the heat shock
In this study, we isolated and characterized B. abortusRA1, a transposon-derived rough mutant of standard strain 2308. We further identified the gene disrupted by Tn5 in strain RA1 as wboA, which encodes a putative glycosyltransferase, an enzyme which appears to be essential for the biosynthesis of the O side chain in B. abortus. LPS analysis of strains RA1 and RB51 indicated that both strains lack the O side chain. However, the reason for the presence of the intermediate LPS band in strain RA1 that is lacking in strain RB51 is not known (Fig. 1). One possibility is that the mutation in strain RA1 results in exposure of a glycose that can be glycosylated by glycosyltransferases that are not able to attach specific sugars in the parent or in strain RB51. The result may be a side branch in the core, generating a new glycoform. Evidence for this is the relatively high amount of galactose present in RA1, whereas only a trace amount of this glycose is present in RB51. Unfortunately, the nature of the ...
Brucella bacteria bacteria are found in cattle, pigs, goats, sheep, working dogs and domestic animals mainly overseas - risk to travellers
Neurobrucellosis, which is the most morbid form of brucellosis disease, presents with inflammatory signs and symptoms. Recent experimental evidence clearly indicates that deregulation of astrocytes and microglia caused by Brucella infection creates a microenvironment in the central nervous system (CNS) in which secretion of pro-inflammatory mediators lead to destabilization of the glial structure, the damage of the blood brain barrier (BBB) and neuronal demise. This review of Brucella interactions with cells of the CNS and the BBB is intended to present recent immunological findings that can explain, at least in part, the basis for the inflammatory pathogenesis of the nervous system that takes place upon Brucella infection. [Cerrar ...
Brucella species are small, coccobacillary, Gram-negative rods that morphologically resemble Haemophilus and Bordetella. They are nonmotile, non-acid-fast, and non-spore-forming. The cells have a typical Gram-negative structure, and the outer membrane contains proteins. The genus Brucella contains nine closely related variants that differ primarily in their preferred terrestrial or marine hosts. Taxonomists vacillate as to whether they should be called species or something else. The three most commonly infecting humans, B abortus (cattle), B melitensis (sheep, goats), and B suis (swine), will all be referred to here as Brucella abortus or simply Brucella ...
Brucella spp. are zoonotic Gram-negative intracellular pathogens with the ability to survive and replicate in phagocytes. It has been shown that bacterial proteins expressed abundantly in this niche are stress-related proteins capable of triggering effective immune responses. BMEI1549 is a molecular chaperone designated DnaK that is expressed under stress conditions and helps to prevent formation of protein aggregates. In order to study the potential of DnaK as a prospective Brucella subunit vaccine, immunogenicity and protective efficacy of recombinant DnaK from Brucella melitensis was evaluated in BALB/c mice. The dnak gene was cloned, expressed in Escherichia coli, and the resulting recombinant protein used as subunit vaccine. DnaK-immunized mice showed a strong lymphocyte proliferative response to in vitro antigen stimulation. Although comparable levels of antigen-specific IgG2a and IgG1 were observed in immunized mice, high amounts of IFN-?, IL-12 and IL-6, no detectable level of IL-4 and ...
The innate immune system detects the presence of microbes in tissue by pattern recognition of conserved microbial structures, known as pathogen-associated molec...
Although not all of the differentiating genes identified were transcribed under steady state conditions, a group of genes sufficient to discriminate unambiguously between B. suis, B. melitensis, and B. abortus was identified. We present an overview of these genomic differences and the use of these f …
En tres municipios de Córdoba (Andalucía, España) se identificaron once casos de brucelosis. Se realizó un estudio de casos y controles, seleccionando tres casos por cada control. Las personas que consumieron queso de cabra fresco sin higienizar elaborado en una granja situada en el territorio epidémico, presentaban un mayor riesgo de padecer brucelosis (OR=21,6, IC 95%=1,6-639,8). Se identificó Brucella melitensis serotipo 3 en muestras clínicas, y en tejido de cabras y muestras de leche de la granja del rebaño. Se adoptaron medidas preventivas y el brote fue contenido tras la retirada del mercado de todos los quesos sospechosos, la desinfección y limpieza adicional de la granja y la realización de actividades de promoción sanitaria.
Bacillus anthracis is a facultative intracellular bacterial pathogen that can cause cutaneous, gastrointestinal or respiratory disease in many vertebrates, including humans. Commercially available anthrax vaccines for immunization of humans are of limited duration and do not protect against the respiratory form of the disease. Brucella abortus is a facultative intracellular bacterium that causes chronic infection in animals and humans. As with other intracellular pathogens, cell mediated immune responses (CMI) are crucial in affording protection against brucellosis. B. abortus strain RB51 has been shown to be useful in eliciting protective cell mediated immunity and humoral responses against Brucella in cattle and other animal species. Since the protective antigen (PA) of B. anthracis is known to induce protective antibodies, it was decided that the objective of this research was to test whether the gene encoding PA could be expressed in Brucella producing a bivalent vaccine to protect against ...
Serological test is one of diagnostic method to detect pathogenicity brucella. Several methods are being improving such as Rose Bengal Test (RBT), Complement Fixation Test (CFT) dan Enzyme-linked Immunosorbent Assay (ELISA). Immunostick has an accuracy equivalent to ELISA and is easy to apply in the field so it is possible to be applied as a rapid test for brucellosis detection. The study aim was to know sensitivity and specificity of immunostick that were used to detecte antibody Brucella abortus using commercial antigens of B. abortus Strain 19 (S19) and B. abortus Strain 99 (S99). The test have compared with ELISA. The tests were conducted in two stages, namely (i) immunostick ability to detect antibodies in seropositive and seronegative serum, and (ii) the immunostick result were compared with ELISA result in serum grup that were be know and unknown status. A total of 250 serums were examined and result indicated that immunostick can be detect B. abortus antibodies in cattle serum with ...
Brucella melitensis bacteria cause persistent, intracellular infections in small ruminants as well as in humans, leading to significant morbidity and economic loss worldwide. The majority of experiments on the transcriptional responses of Brucella to conditions inside the host have been performed following invasion of cultured mammalian cells, and do not address gene expression patterns during long-term infection. Here, we examine the application of the previously developed coincidence cloning methodology to recover and characterize B. melitensis RNA from the supramammary lymph node of experimentally-infected goats. Using coincidence cloning, we successfully recovered Brucella RNA from supramammary lymph nodes of B. melitensis-infected goats at both short-term (4 weeks) and long-term (38 weeks) infection time points. Amplified nucleic acid levels were sufficient for analysis of Brucella gene expression patterns by RNA-sequencing, providing evidence of metabolic activity in both the short-term and the
We present a rare case of brucella endocarditis, forming a vegetation on the mitral valve. The definitive diagnosis has been made with clinical suspicion, positive serology, the demonstration of the vegetation with the echocardiography and with the production from the multiple blood culture of brucella melitensis and from the excised valve. Our patient has been successfully treated with specific antibiotherapy and the surgery of replacement of mitral valve. Our aim in presenting the case is to remind the infective endocarditis which is due to this factor in the regions like our country which is endemic for ...
Curated}} {{Biorealm Genus}} [[Image:brucella_close_up.JPG,thumb,400px,right,Electron micrograph of B. abortus located inside cisternae of Vero cell. Within the perinuclear envelope, the cisternae containing B. abortus are discontinuously lined by ribosomes (arrows). From [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2114362 Detilleux et al.]]] ==Classification== ===Higher order taxa:=== Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Brucellaceae ===Species:=== Brucella abortus, Brucella canis, Brucella cetaceae, Brucella maris , Brucella melitensis, Brucella pinnipediae, Brucella sp. {, , height=10 bgcolor=#FFDF95 align=center , NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=234 Taxonomy] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=genomeprj&cmd=Search&dopt=DocSum&term=txid234%5BOrganism:exp%5D Genomes] ,} ==Description and Significance== Brucella sp. causes brucellosis, ...
In this study, we explore the regulatory roles of pro-inflammatory cytokine tumor necrosis factor alpha (TNF) in the innate immunity of macrophages against B. abortus infection. We show that infection of macrophage with B. abortus induces marked expression and secretion of TNF which subsequently binds to TNF receptor 1 (TNFR-1) and activates a downstream signaling cascade of the innate immunity. Blocking of TNF signaling resulted in a notable increase of B. abortus survival which was associated with an increase of anti-inflammatory cytokine interleukin 10 (IL-10), a beneficial effector of Brucella survival, as well as remarkable decrease of reactive oxygen species (ROS) and nitric oxide (NO), antibrucella molecules ...
The patterns of susceptibility to hydrophobic and hydrophilic drugs and the uptake of the fluorescent probe N-phenyl-naphthylamine in Brucella spp., Haemophilus influenzae, Escherichia coli, and deep rough Salmonella minnesota mutants were compared. The results show that the outer membranes of smooth and naturally rough Brucella spp. do not represent barriers to hydrophobic permeants and that this absence of a barrier relates at least in part to the properties of Brucella lipopolysaccharide. ...
What is brucellosis? Brucellosis is an infectious disease caused by the bacteria of the genus Brucella. These bacteria are primarily passed among animals, and they cause disease in many different vertebrates. Various Brucella species affect sheep, goats, cattle, deer, elk, pigs, dogs, and several other animals. Humans become infected by coming in contact with animals or animal products that are contaminated with these bacteria. In humans brucellosis can cause a range of symptoms that are similar to the flu and may include fever, sweats, headaches, back pains, and physical weakness. Severe infections of the central nervous systems or lining of the heart may occur. Brucellosis can also cause long-lasting or chronic symptoms that include recurrent fevers, joint pain, and fatigue.. Back to Top How common is brucellosis? Brucellosis is not very common in the United States, where100 to 200 cases occur each year. But brucellosis can be very common in countries where animal disease control programs have ...
Designing a potent recombinant vaccine, using the appropriate subunits with the greatest effect on stimulating the immune system, especially in the case of intracellular pathogens such as gram negative Brucella Melitensis bacteria, is of great importance. In this study, three repeats of 27 amino acids of the immunogenic epitope derived from OMP31 antigen (3E) from the Brucella melitensis, in a protective manner against Brucellosis have been used. To fortify the delivery system of recombinant antigens, IL-2 cytokine as a molecular adjuvant was fused to recombinant constructs. Recombinant proteins were evaluated for immunological studies in a mouse model (BALB/c). The results showed that all recombinant proteins could stimulate the immune system to produce Th1 cytokines and antibodies in compare to the negative control treatments. 3E-IL2 and then OMP31-IL2 proteins stimulated higher levels of IFN-γ and IL-2 compared to the other treatments (p | 0.05). Also, the results indicated that experimental
Diagnosis Code A23.0 information, including descriptions, synonyms, code edits, diagnostic related groups, ICD-9 conversion and references to the diseases index.
Looking for online definition of abortus in the Medical Dictionary? abortus explanation free. What is abortus? Meaning of abortus medical term. What does abortus mean?
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Pathway modules Carbohydrate metabolism Central carbohydrate metabolism M00002 Glycolysis, core module involving three-carbon compounds [PATH:bmf00010 bmf01200 bmf01230 bmf01100 ...
PCR-RFLP analysis of Brucella LPS genes manAO-Ag, manBO-Ag, wbkD, wbkF, wboA and wa**. Panel A. Lanes: 1, molecular size markers; 2, manAO-Ag from B. melitensis
Doctors usually confirm a diagnosis of brucellosis by testing blood or bone marrow for the brucella bacteria or by testing blood for antibodies to the bacteria. To help detect complications of brucellosis, your doctor may order additional tests, including: X-rays. X-rays can reveal changes in your bones and joints ...
When and how often laboratory tests are done may depend on many factors. The timing of laboratory tests may rely on the results or completion of other tests, procedures, or treatments. Lab tests may be performed immediately in an emergency, or tests may be delayed as a condition is treated or monitored. A test may be suggested or become necessary when certain signs or symptoms appear. Due to changes in the way your body naturally functions through the course of a day, lab tests may need to be performed at a certain time of day. If you have prepared for a test by changing your food or fluid intake, lab tests may be timed in accordance with those changes. Timing of tests may be based on increased and decreased levels of medications, drugs or other substances in the body. The age or gender of the person being tested may affect when and how often a lab test is required. Chronic or progressive conditions may need ongoing monitoring through the use of lab tests. Conditions that worsen and improve may ...
This is the microbiology questions and answers section on Brucella with explanation for various interview, competitive examination and entrance test. Solved examples with detailed answer description, explanation are given and it would be easy to understand.
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Pei J, Ficht TA (January 2004). "Brucella abortus rough mutants are cytopathic for macrophages in culture". Infection and ... mutant as a new live rough-phenotype vaccine". Infection and Immunity. 71 (11): 6264-9. doi:10.1128/iai.71.11.6264-6269.2003. ... Ugalde JE, Czibener C, Feldman MF, Ugalde RA (October 2000). "Identification and characterization of the Brucella abortus ... Ugalde JE, Comerci DJ, Leguizamón MS, Ugalde RA (November 2003). "Evaluation of Brucella abortus phosphoglucomutase (pgm) ...
He helped develop a genetically engineered vaccine against anthrax. He is an elected fellow of several major Indian science ... His research group has initiated research in other important infectious disease systems like Mycobacterium, Brucella; aiming to ... His team has also developed a DNA vaccine against Rabies. ... Recombinant Vaccine Development, and Programmed Cell Death in ...
Currently, all cattle of ages 3-8 months must receive the Brucella abortus strain 19 vaccine. Australia is free of cattle ... Brucella species survive well in aerosols and resist drying. Brucella and all other remaining biological weapons in the U.S. ... Brucella suis, also referred to as swine brucellosis Wyatt HV (2014). "How did Sir David Bruce forget Zammit and his goats ?" ( ... with the Brucella milk ring test. Cows confirmed to be infected are often killed. In the United States, veterinarians are ...
"Escheriosomes entrapped DNA vaccine co-expressing Cu-Zn superoxide dismutase and IL-18 confers protection against Brucella ... Vaccine. 2012, 6;30(48):6851-8. doi: 10.1016/j.vaccine.2012.09.010. Ansari MA, Zubair S, Mahmood A, Gupta P, Khan AA, Gupta UD ... Vaccine. 2006, 13;24(7):948-56. doi: 10.1016/j.vaccine.2012.09.010. Chauhan A, Zubair S, Tufail S, Sherwani A, Sajid M, Raman ... Vaccine. 2011, 24;29(29-30):4720-7. doi: 10.1016/j.vaccine.2011.04.088. Mallick AI, Singha H, Chaudhuri P, Nadeem A, Khan SA, ...
A Brucella DNA vaccine containing IL-15 gene enhanced the CD8+ T cell immune response in mice. IL-15 was needed for CD4+ T cell ... "An IL-15 adjuvant enhances the efficacy of a combined DNA vaccine against Brucella by increasing the CD8+ cytotoxic T cell ... doi:10.1016/j.vaccine.2009.12.076. PMID 20064480. Hock K, Laengle J, Kuznetsova I, Egorov A, Hegedus B, Dome B, Wekerle T, ... Preclinical results of NDV-modified tumor vaccine showed promise by controlling melanoma tumor growth in mice. A recombinant ...
Sub-unit vaccine candidates, such as killed-whole cell vaccines, are also under investigation, however research has not reached ... Cross reactivity with Brucella can confuse interpretation of the results, so diagnosis should not rely only on serology. ... approved vaccines against tularemia. However, vaccination research and development continues, with live attenuated vaccines ... 2000). Manual of standards for diagnostic tests and vaccines: lists A and B diseases of mammals, birds and bees. Paris, France ...
Some of these materials were used for Iraq's biological weapons research program, while others were used for vaccine ... These materials included anthrax, West Nile virus and botulism, as well as Brucella melitensis, and Clostridium perfringens. ... Approximately 150,000 US troops received the U.S. Food and Drug Administration-licensed anthrax vaccine (BioThrax), and 8,000 ... and new research activities involving Brucella and Crimean-Congo hemorrhagic fever virus. Despite diligent investigations since ...
A new type of vaccine Charles Pilet and Marc Bonneau are creating a new type of vaccine from a strain of Brucella abortus made ... An improved vaccine control method The study of several mouse lines showed the sensitivity of some of these lines to Brucella ... This type of vaccine also confirmed the essentially cellular nature of brucella immunity and the passive role of antibodies. A ... which made it possible to propose a new method for controlling brucella vaccines, a method that has since been adopted ...
Offit, Paul A. (2007-09-01). The Cutter Incident: How America's First Polio Vaccine Led to the Growing Vaccine Crisis. Yale ... antitumor processes of Brucella, and numerous contributions to cold adapted influenza virus. Youngner met and married his first ... The Salk vaccine is based upon formalin inactivated wild type virus. The key to effective inactivation depended upon a color ... Applying this method to a single kidney "could produce enough raw material for 6000 shots of polio vaccine." This advance in ...
A Brucella DNA vaccine containing IL-15 gene enhanced the CD8+ T cell immune response in mice.[32] IL-15 was needed for CD4+ T ... "An IL-15 adjuvant enhances the efficacy of a combined DNA vaccine against Brucella by increasing the CD8+ cytotoxic T cell ... doi:10.1016/j.vaccine.2009.12.076. PMID 20064480.. *^ Hock K, Laengle J, Kuznetsova I, Egorov A, Hegedus B, Dome B, Wekerle T, ... Vaccines AdjuvantsEdit. Vector-based therapy - Nonlytic Newcastle Disease Virus (NDV) was engineered to express recombinant IL- ...
J07BX01 Smallpox vaccines J07BX02 Ebola vaccines J07BX03 Covid-19 vaccines J07CA01 Diphtheria-poliomyelitis-tetanus J07CA02 ... J07AC01 Anthrax antigen J07AD01 Brucella antigen J07AE01 Cholera, inactivated, whole cell J07AE02 Cholera, live attenuated ... ATC code J07 Vaccines is a therapeutic subgroup of the Anatomical Therapeutic Chemical Classification System, a system of ... C Empty group Vaccines for veterinary use are in the ATCvet group QI. "ATC (Anatomical Therapeutic Chemical Classification ...
... anthrax vaccines MeSH D20.215.894.135.134 - brucella vaccine MeSH D20.215.894.135.225 - cholera vaccines MeSH D20.215.894.135. ... hepatitis a vaccines MeSH D20.215.894.899.955.400 - hepatitis b vaccines MeSH D20.215.894.899.970 - yellow fever vaccine MeSH ... measles vaccine MeSH D20.215.894.899.404.500 - measles-mumps-rubella vaccine MeSH D20.215.894.899.488 - mumps vaccine MeSH ... rubella vaccine MeSH D20.215.894.899.779.500 - measles-mumps-rubella vaccine MeSH D20.215.894.899.790 - saids vaccines MeSH ...
... at Texas A&M University and her team claim to be close to creating a human vaccine. It would primarily be used to immunize ... Brucella abortus A13334, 3,401 ORFs Brucella canis ATCC 23365, 3,408 ORFs Brucella melitensis 16M, 3,279 ORFs Brucella microti ... 3,193 ORFs Brucella pinnipedialis B2/94, 3,505 ORFs Brucella suis 1330, 3,408 ORFs Genome data for these and other Brucella ... Brucella species have been found primarily in mammals: Pathogenic Brucella species can cause abortion in female animals by ...
The Brucella abortus (rough LPS Brucella) vaccine, developed for bovine brucellosis and licensed by the USDA Animal Plant ... Since brucellosis threatens the food supply and causes undulant fever, Brucella suis and other Brucella species (B. melitensis ... of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella ... Brucella suis is a bacterium that causes swine brucellosis, a zoonosis that affects pigs. The disease typically causes chronic ...
Dorneles, EM; Sriranganathan, N; Lage, AP (8 July 2015). "Recent advances in Brucella abortus vaccines". Veterinary Research. ... Brucella has twelve different kinds of species, one being Brucella abortus. Some of the other species are known as B. ... "Humans and Brucella Species". "Brucellosis: Brucella Abortus" (PDF). Kaden, R.; Ferrari, S.; Jinnerot, T.; Lindberg, M.; Wahab ... Brucella abortus is a Gram-negative alpha-proteobacterium in the family Brucellaceae and is one of the causative agents of ...
"Serological Diagnosis of Brucella Infections in Odontocetes". Clinical and Vaccine Immunology. 16 (6): 906-915. doi:10.1128/CVI ... LPSN "Brucella inopinata" at the Encyclopedia of Life Type strain of Brucella inopinata at BacDive - the Bacterial Diversity ... Brucella inopinata is a Gram-negative, nonmotile, non-spore-forming coccoid bacterium, first isolated from a breast implant ... "Taxonomy of Brucella." Open Veterinary Science Journal 4.1 (2010): 85-101. Fischer, Dominik; Lorenz, Nadja; Heuser, Wenke; ...
"Serological diagnosis of Brucella infections in odontocetes". Clinical and Vaccine Immunology. 16 (6): 906-15. doi:10.1128/CVI. ... LPSN "Brucella ceti" at the Encyclopedia of Life NOAA informative brochure Type strain of Brucella ceti at BacDive - the ... Brucella ceti is a gram negative bacterial pathogen of the Brucellaceae family that causes brucellosis in cetaceans. Brucella ... and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts". International ...
Brucella+vaccine at the US National Library of Medicine Medical Subject Headings (MeSH) Marta A. Guerra; Barun K. De. " ... Brucellosis vaccine is a vaccine for cattle, sheep and goats used against brucellosis. It is an attenuated vaccine based on a ... Current Understanding and Future Approaches to Vaccine Development for Mice and Humans". Clin. Microbiol. Rev. 16 (1): 65-78. ... modified brucellosis bacteria.[citation needed] Currently, there is no vaccine available for humans. Ko J, Splitter GA (January ...
Vaxign has been tested in different pathogen models, including uropathogenic E. coli and Brucella spp. VIOLIN also maintains ... VIOLIN currently contains over 3,000 vaccines or vaccine candidates for over 190 pathogens. The vaccine information in the ... Different types of information is curated, including vaccine name, license status, antigens used, vaccine adjuvants, vaccine ... All vaccine information in the VIOLIN vaccine database is supported by quoted references. The data generated by a curator is ...
... several species of Brucella, chikungunya, yellow fever virus, West Nile virus, Yersinia pestis, and SARS-CoV-2. Biosafety level ... aerosol-transmitted within the laboratory and cause severe to fatal disease in humans for which there are no available vaccines ...
... and Brucella. In Alphaproteobacteria, methylation of adenine regulates the cell cycle and couples gene transcription to DNA ... the bacterium to randomly change its characteristics into six alternative states that could pave the way to improved vaccines. ...
Aside from these bacteria, PPIB has been identified in Brucella abortus, Mycobacterium tuberculosis, Bacillus subtilis and ... recognized by HLA-A24-restricted and tumor-specific cytotoxic T lymphocytes which could be used as cancer vaccines, and in fact ... "Intracellularly Induced Cyclophilins Play an Important Role in Stress Adaptation and Virulence of Brucella abortus". Infection ...
In the US an anthrax vaccine, Anthrax Vaccine Adsorbed (AVA) exists and requires five injections for stable use. Other anthrax ... Brucellosis (Brucella species) Epsilon toxin of Clostridium perfringens Food safety threats (for example, Salmonella species, E ... An anthrax vaccine does exist but requires many injections for stable use. When discovered early, anthrax can be cured by ... These vaccine strains are missing the genes that cause a symptomatic response. In September and October 2001, several cases of ...
... wild or vaccine types) Rabies virus (classical rabies and rabies-related lyssaviruses) Rickettsia spp Rift Valley fever virus ... Bordetella pertussis Borrelia spp Brucella spp Burkholderia mallei Burkholderia pseudomallei Campylobacter spp Chikungunya ...
A laboratory on vaccine and serum research was also established near Moscow in 1928, within the Military Chemical Agency. This ... Brucella spp (brucellosis) Coxiella burnetii (Q-fever) Venezuelan equine encephalitis virus (VEE) Botulinum toxin (botulism) ...
... a virus in a host that it wouldn't usually infect in order to generate attenuated strains for use in vaccines. This was done ... and infections of lab workers with Yersinia pestis and Brucella species. In an interview with the New York Times, Richard H. ... Fisman of the University of Toronto Alison Galvani of Yale School of Public Health Peter Hale of the Foundation for Vaccine ...
No vaccine is available. There are several Shigella vaccine candidates in various stages of development that could reduce the ... Girard MP, Steele D, Chaignat CL, Kieny MP (April 2006). "A review of vaccine research and development: human enteric ... infections". Vaccine. 24 (15): 2732-50. doi:10.1016/j.vaccine.2005.10.014. PMID 16483695.. ...
The first conjugate vaccine was licensed in 2005, the second in 2010. Conjugate vaccines are the preferred vaccine for people 2 ... "Vaccine. 27 (Suppl 2): B71-7. doi:10.1016/j.vaccine.2009.04.070. PMC 2712446 . PMID 19477055.. ... Some of the vaccines cover serogroup B, while others cover A, C, W, and Y.[26] A meningococcal polysaccharide vaccine (MPSV4) ... Main article: Meningococcal vaccine. United States[edit]. A number of vaccines are available in the U.S. to prevent ...
Hassani M, Patel MC, Pirofski LA (April 2004). "Vaccines for the prevention of diseases caused by potential bioweapons". ... Agents considered for weaponization, or known to be weaponized, include bacteria such as Bacillus anthracis, Brucella spp., ... Would demonstrate how to render a vaccine ineffective; Would confer resistance to therapeutically useful antibiotics or ... non-availability of vaccines and availability of an effective and efficient delivery system. Stability of the weaponized agent ...
Subsequently, a vaccine for horses was developed. And it turned out that the isolation of the virus by Meyer led to the ... He suggested classing the various species of bacteria into one family (genus), formally named Brucella, to honor the discoverer ... During the years of World War II a vaccine against yellow fever was used in the Armed Forces that produced (unexpectedly) ... It was actually manufactured at the Hooper when the Army needed vaccines to protect the troops in Vietnam (one endemic area ...
Use in vaccinesEdit. The first vaccine against smallpox by Edward Jenner in 1800 was by infection of a zoonotic bovine virus ... Brucella spp. cattle, goats infected milk or meat Bubonic plague, Pneumonic plague, Septicemic plague, Sylvatic plague Yersinia ...
Magistro, G; Stief, CG (6 August 2018). "Vaccine Development for Urinary Tract Infections: Where Do We Stand?". European ... Huttner, A; Gambillara, V (24 May 2018). "The development and early clinical testing of the ExPEC4V conjugate vaccine against ... A number of vaccines are in development as of 2018.[56][57] ... Brucella abortus *Brucellosis. Bartonellaceae. *Bartonellosis: ...
... and Brucella. In Alphaproteobacteria, methylation of adenine regulates the cell cycle and couples gene transcription to DNA ... the bacterium to randomly change its characteristics into six alternative states that could pave the way to improved vaccines. ...
"Vaccine. 32 (14): 1579-87. doi:10.1016/j.vaccine.2013.08.067. PMC 4682887. PMID 24016806.. ... A vaccine for gonorrhea has been developed that is effective in mice.[77] It will not be available for human use until further ... Development of a vaccine has been complicated by the ongoing evolution of resistant strains and antigenic variation (the ... A study published in 2017 showed that MeNZB group B meningococcal vaccine provided a partial protection against gonorrhea.[78] ...
The World Health Organization (WHO) has prequalified three bivalent cholera vaccines-Dukoral (SBL Vaccines), containing a non- ... The vaccine that the US Food and Drug Administration (FDA) recommends, Vaxchora, is an oral attenuated live vaccine, that is ... "History of Vaccines. Retrieved 2021-01-20.. *^ See: *Fillipo Pacini (1854) "Osservazioni microscopiche e deduzioni patologiche ... doi:10.1016/j.vaccine.2004.11.044. PMID 15780724.. *^ Choe C, Raschky PA (January 2016). "Media, institutions, and government ...
"Vaccines for preventing typhoid fever". Cochrane Database Syst Rev (3): CD001261. PMID 17636661. ഡി.ഒ.ഐ.:10.1002/14651858. ... Brucella abortus (Brucellosis). Bartonellaceae. Bartonellosis: Bartonella henselae (പൂച്ചമാന്തി രോഗം) · Bartonella quintana ( ... Typhoid polysaccharide vaccine) (ടൈയ്ഫിം വി(Typhim Vi), ടൈയ്ഫറിക്സ്(Typherix) എന്നീ പേരുകളിൽ വിൽക്കപ്പെടുന്നു.) ടൈയ്ഫോഡ് പടർ ...
The oral cholera vaccine, while effective for prevention of cholera, is of questionable use for prevention of TD.[6] A 2008 ... Jelinek T, Kollaritsch H (2008). "Vaccination with Dukoral against travelers' diarrhea (ETEC) and cholera". Expert Rev Vaccines ... Ahmed, T; Bhuiyan, TR; Zaman, K; Sinclair, D; Qadri, F (5 July 2013). "Vaccines for preventing enterotoxigenic Escherichia coli ... Several vaccine candidates targeting ETEC or Shigella are in various stages of development.[27][28] ...
Vaccines are currently available against all six strains, including the newest vaccine against serogroup B. The first vaccine ... are the commonly used vaccines. Vaccines offer significant protection from three to five years (plain polysaccharide vaccine ... Although conjugate vaccines are the preferred meningococcal vaccine in adolescents 11 years of age or older, polysaccharide ... Although conjugate vaccines are the preferred meningococcal vaccine in adults 55 years of age or younger, polysaccharide ...
Prevention is through public health measures such as not handling dead animals in areas where plague is common.[7] [1] Vaccines ...
A typhoid vaccine can prevent about 30% to 70% of cases during the first two years.[7] The vaccine may have some effect for up ... Two typhoid vaccines are licensed for use for the prevention of typhoid:[7] the live, oral Ty21a vaccine (sold as Vivotif by ... The alternative Ty21a vaccine is recommended for people 5 years or older, and has a 5-7-year duration with a 51-67% vaccine ... "Vaccine. 33: C55-C61. doi:10.1016/j.vaccine.2015.04.028. Retrieved 2016-12-15.. ...
... and a successful vaccine has yet to be developed as of 2016[update].[60] ... "Vaccination of Elk (Cervus canadensis) with Brucella abortus Strain RB51 Overexpressing Superoxide Dismutase and ... Glycosyltransferase Genes Does Not Induce Adequate Protection against Experimental Brucella abortus Challenge". Frontiers in ...
"Plague Vaccine". CDC. June 11, 1982. Retrieved Apr 30, 2015.. *^ Jefferson T, Demicheli V, Pratt M; Demicheli; Pratt (2000). ... "Vaccines for preventing plague". Cochrane Database Syst Rev (2): CD000976. doi:10.1002/14651858.CD000976. PMID 10796565.. CS1 ... Main article: Plague vaccine. Since human plague is rare in most parts of the world, routine vaccination is not needed other ... by the Cochrane Collaboration found no studies of sufficient quality to make any statement on the efficacy of the vaccine.[19] ...
While no vaccine is available specifically for paratyphoid, the typhoid vaccine may provide some benefit.[1][2] Prevention ... Those who travel to countries with poor sanitation should receive a live attenuated typhoid vaccine-Ty21a (Vivotif), which, in ... Whitaker, J. A.; Franco-Paredes, C.; Del Rio, C.; Edupuganti, S. (2009). "Rethinking Typhoid Fever Vaccines: Implications for ... Control requires treatment of antibiotics and vaccines prescribed by a doctor. Major control treatments for paratyphoid fever ...
The ovine Cff vaccine has likewise been found ineffective in preventing future infections in ewes given that it does not ... of the deceased fetus causes necrosis of the placenta and placental lesions are not easily distinguished from Brucella abortus ... As vaccines are typically not efficient in preventing future spread, infected bulls are often culled. Human infections may be ... Vaccines can be used to reduce the occurrence of campylobacteriosis but will not eradicate infections. For example, vaccination ...
"A brief history of vaccines and how they changed the world". World Economic Forum. Retrieved 2020-04-30. "Creating a world in ... Brucella species (brucellosis) Burkholderia pseudomallei (melioidosis) Burkholderia mallei (glanders) Coxiella burnetii (Q ... The proposed interventions were based on the following: the U.S. public health system, research and training, vaccine and drug ... such as vaccine-derived poliovirus The 1992 IOM report distinguished 6 factors contributing to emergence of new diseases ( ...
In the last several decades, much research has been performed to develop safer Brucella vaccines to control the disease mainly ... Till now, no effective human vaccine is available. The aim of this paper is to review and … ... A history of the development of Brucella vaccines Biomed Res Int. 2013;2013:743509. doi: 10.1155/2013/743509. Epub 2013 Jun 3. ... The aim of this paper is to review and discuss the importance of methodologies used to develop Brucella vaccines in pursuing ...
At the beginning of this century in the US, Brucella abortus is a problem in elk and bison in the Greater Yellowstone Area, B. ... Brucella vaccines in wildlife Vet Microbiol. 2002 Dec 20;90(1-4):533-44. doi: 10.1016/s0378-1135(02)00233-x. ... This manuscript reviews research on the use of Brucella vaccines in species of wildlife with emphasis on safety and efficacy. ... The use of vaccines to control brucellosis in populations of wildlife and therefore reducing the risk of transmission to humans ...
The patient reported having sustained a needle stick while administering the Brucella abortus strain RB51 vaccine (RB51) to ... Notes from the field: Brucella abortus vaccine strain RB51 infection and exposures associated with raw milk consumption-Wise ... Notes from the Field: Adverse Event Associated with Unintentional Exposure to the Brucella abortus RB51 Vaccine - Oregon, ... Adverse events in humans associated with accidental exposure to the livestock brucellosis vaccine RB51. Vaccine 2004;22:3435-9 ...
... sequencing to rapidly and comprehensively determine the complete genome sequence of the attenuated Brucella abortus vaccine ... has been used as a vaccine strain in vaccination of cattle against brucellosis for six decades. Despite many studies, the ... The Brucella abortus strain S19, a spontaneously attenuated strain, ...
Global Brucella Abortus Vaccines Industry 2017 Market Research Report with 154 pages available at USD 2800 for single User PDF ... Part I Brucella Abortus Vaccines Industry Overview. Chapter One Brucella Abortus Vaccines Industry Overview. 1.1 Brucella ... 1.5 Brucella Abortus Vaccines Application Analysis. 1.5.1 Brucella Abortus Vaccines Main Application Analysis. 1.5.2 Brucella ... Part II Asia Brucella Abortus Vaccines Industry. Chapter Two Asia Brucella Abortus Vaccines Product History of Development. ...
Results show that the seroconversion induced by Brucella abortus RB51 and RB51-SOD vaccines is lower than that by Brucella ... in a goat flock and the seroconversion of three groups of animals vaccinated with Rev-1 (Brucella melitensis), RB51, and RB51- ... SOD (Brucella abortus) to estimate the level of protection conferred on susceptible females. Seventy-two animals were used by ... The aim of this study was to determine the seroprevalence of Brucella spp. ...
... were given a single oral dose of at least 1.0X1010 colony-forming units of Brucella abortus strain RB51 vaccine (RB51). Animals ... Should this occur, non-target species other than elk and bison may come in contact with the vaccine resulting in morbidity or ... It is possible that an oral brucellosis vaccine could be developed and disseminated in the GYA to reduce disease transmission. ... Safety Of Brucella Abortus Strain Rb51 Vaccine In Non-Target Ungulates And Coyotes ...
Thank you for sharing this Clinical and Vaccine Immunology article.. NOTE: We request your email address only to inform the ... Fluorescence Polarization Assay for Detection of Brucella abortus Antibodies in Bulk Tank Bovine Milk Samples. D. Gall, K. ... A simple, rapid, inexpensive fluorescence polarization assay for the detection of antibodies to Brucella abortus in bulk tank ... Fluorescence Polarization Assay for Detection of Brucella abortus Antibodies in Bulk Tank Bovine Milk Samples ...
Evaluation of Vaccination with Brucella abortus S19 Vaccine in Cattle Naturally Infected with Brucellosis in Productive Systems ... Therefore, the objective of this study was to evaluate Brucella abortus S19 vaccine in cattle naturally infected with ... Efficacy of vaccination with Brucella abortus S19 vaccine as control measure against bovine brucellosis has been controversial ... Evaluation of Vaccination with Brucella abortus S19 Vaccine in Cattle Naturally Infected with Brucellosis in Productive Systems ...
Microbial Immunity and Vaccines. Intraspleen Delivery of a DNA Vaccine Coding for Superoxide Dismutase (SOD) of Brucella ... Vaccination with Brucella abortus rough mutant RB51 protects BALB/c mice against virulent strains of Brucella abortus, Brucella ... A DNA vaccine encoding Cu-Zn superoxide dismutase of Brucella abortus induces protective immunity in BALB/c mice. Infect. Immun ... Induction of immune response in BALB/c mice with a DNA vaccine encoding bacterioferritin or P39 of Brucella spp. Infect. Immun. ...
Thank you for sharing this Clinical and Vaccine Immunology article.. NOTE: We request your email address only to inform the ... The Brucella IgM and IgG flow assays: physical presentation, components and method. The Brucella IgM and IgG flow assays (A) ... Comparison of the Brucella Standard Agglutination Test with the ELISA IgG and IgM in patients with Brucella bacteremia. Diagn. ... LPS was prepared from a solid 3-day-old culture of Brucella abortus strain 1119-3 grown on Brucella agar in 250-ml culture ...
Evaluation of unmarked deletion mutants as improved Brucella vaccine strains in the mouse and goat models. by Kahl, Melissa ... Historical data suggests that prolonged survival of Brucella vaccine organisms in the target host enhances immune protection. ... appear superior as a vaccine candidate compared to approved vaccine strains S19 and Rev1 in the mouse model against either ... We hypothesize that in order for a vaccine to be efficacious, it must survive in the host. In order to test this, we ...
To determine the placental tropism and abortigenicity of the vaccine candidate Brucella abortus strain RB51 (SRB51), a rough ... Recent advances in Brucella abortus vaccines. *Elaine Maria Seles Dorneles, Nammalwar Sriranganathan, Andrey P Lage ... Epidemiological study of Brucellosis in cattle, immunized with Brucella abortus RB51 vaccine in endemic zones.. *Enrique Jaime ... To determine the placental tropism and abortigenicity of the vaccine candidate Brucella abortus strain RB51 (SRB51), a rough ...
... ... Commercially available anthrax vaccines for immunization of humans are of limited duration and do not protect against the ... Brucella abortus is a facultative intracellular bacterium that causes chronic infection in animals and humans. As with other ... objective of this research was to test whether the gene encoding PA could be expressed in Brucella producing a bivalent vaccine ...
An anti-Brucella vaccine candidate which comprises of purified Brucella lipopolysaccharide and cocktail of four Salmonella ... Effect of immunization routes and protective efficacy of Brucella antigens delivered via Salmonella vector vaccine ... Taken together, each of Brucella antigens delivered by ST successfully induced antigen specific immune response and it was also ... Each vector constitutively express highly conserved Brucella antigens (rB), viz. lumazine synthase (BLS), proline racemase ...
At present, there is no commercially available vaccine for the human brucellosis. Brucella melitensis and Brucella abortus are ... The outer membrane protein 31 (Omp31) and L7/L12 are immunodominant and protective antigens conserved among human Brucella ... compare the immunogenicity and protective efficacy of the L7/L12-TOmp31 construct administered as DNA/DNA and DNA/Pro vaccine ... Brucellosis is one of the most common zoonotic diseases caused by species of Brucella. ...
Brucella melitensis (4), Brucella suis (5), and Brucella abortus (6). Unique fragments occur among the different Brucella ... Multiplex PCR Assay for the Identification and Differentiation of all Brucella Species and the Vaccine Strains Brucella abortus ... Multiplex PCR Assay for the Identification and Differentiation of all Brucella Species and the Vaccine Strains Brucella abortus ... Multiplex PCR Assay for the Identification and Differentiation of all Brucella Species and the Vaccine Strains Brucella abortus ...
However, current vaccine strains including S19 and RB51 are pathogenic to humans and pregnant animals, limiting their use. In ... Therefore, mutant strain ∆22915 could be used as a novel vaccine candidate in the future to protect animals against B. abortus ... Vaccination is an effective strategy for protection against Brucella infection in livestock in developing countries and in ... this study, we constructed the Brucella abortus (B. abortus) S2308 mutant strain Δ22915, in which the putative lytic ...
... a disease for which a specific vaccine is lacking. Attenuated Brucella melitensis Rev 1, used as vaccine against ovine and ... Similarities, but also significant differences, were found regarding the immune response induced by the three vaccines. Mice ... a crucial cytokine in protective immunity against other Brucella species. By contrast, IL-1α -an enhancer of T cell responses ... These characteristics encourage their evaluation in the natural host as homologous vaccines for the specific prophylaxis of B. ...
Development and Evaluation of a DNA Vaccine Encoding 31-KDA Outer Membrane Protein of Brucella Melitensis. Masters thesis, ... Development and Evaluation of a DNA Vaccine Encoding 31-KDA Outer Membrane Protein of Brucella Melitensis ... On the other hand, DNA vaccines have many advantages over traditional protein-based vaccines, such as ease of development, ... On account of the fact that, vaccination is the only way to control and eradicate the disease, the vaccine which can induce ...
Live attenuated Brucella vaccines are considered effective in animals but they may be potentially infectious to humans, so it ... and pcDNA3.1-Omp31 DNA vaccines, native pcDNA3.1/Hygro, PBS, and Rev1 vaccine (five mice per group). Mice immunized with PBS ... Immunogenicity evaluation of plasmids encoding Brucella melitensis Omp25 and Omp31 antigens in BALB/c mice.. Shojaei M1, ... ELISA analysis of IFN-ɣ (A) and IL-10 (B) from BALB/c mice inoculated with DNA vaccines (pcDNA3.1/Hygro-Omp25, pcDNA3.1/Hygro- ...
Brucella challenge dose in guinea pig - posted in Microbiology: Hello, I am going to evaluate the protective efficacy of my ... Flu vaccine produced by a new method protects animals from infection Started by whcaroline, 08 Dec 2016 antibody, vaccine, flu ... New generation vaccine with 3D-printing? Started by henrilentonen, 03 Sep 2018 vaccine * 0 replies ... Also tagged with one or more of these keywords: guinea pig, vaccine, challenge dose, Brucella melitensis. Protocols and ...
The aim of this paper is to review and discuss the importance of methodologies used to develop Brucella vaccines in pursuing ... In the last several decades, much research has been performed to develop safer Brucella vaccines to control the disease mainly ... Till now, no effective human vaccine is available. ...
However, due to some drawbacks shown by these vaccines much effort has been undertaken for the development of new vaccines, ... In this paper, we present a review of the main aspects of the vaccines that have been used in the brucellosis control over the ... Strain 19 and RB51 are the approved B. abortus vaccines strains most commonly used to protect cattle against infection and ... Brucella abortus vaccines play a central role in bovine brucellosis control/eradication programs and have been successfully ...
Clinical and Vaccine Immunology, 14, 299-303. doi: 10.1128/CVI.00350-06 PubMedCrossRefGoogle Scholar ... Prevalence of antibodies to Brucella spp. and individual risk factors in traditional cattle, goats and sheep reared in the ... Evaluation of a fluorescence polarization assay for the detection of serum antibodies to Brucella abortus in water buffalo ( ... Effectiveness of Rose Bengal test and fluorescence polarization assay in the diagnosis of Brucella spp. infections in free ...
Vaccines. Vaccine. Dosage, Administration and Withdrawal Times. Life Stages. Adverse Affects. Brucella melitensis Rev 1 Follow ... Brucella melitensis Rev 1 Follow manufacturers instructions. Breeding male Interferes with serological diagnosis. No Vaccine ... The best available vaccine against B. ovis infection in rams is the live Brucella melitensis strain Rev 1 (Plommet, 1991). A ... New animal vaccines. In: Emel Tümbay, Süleyha Hilmi, Özdem Ang, eds. Brucella and brucellosis in man and animals. Proceedings ...
... foi empregada na detecção de Brucella spp. em 300 amostras de produtos... ... utilizando-se primers desenhados a partir do gene que codifica uma proteína imunogênica de 31 KDa de Brucella abortus, ... Vaccine. Resumo em inglês. A PCR assay using primers designed based on the gene that encodes a 31 KDa immunogenic protein from ... Todas as amostras positivas na PCR para Brucella spp. foram confirmadas como sendo da espécie Brucella abortus pela técnica de ...
Brucella phage lysate bacterin induces elevated TLRs and cytokines response in murine model.(Report) by Journal of Pure and ... Several new generation vaccines like recombinant vaccine (Al-Mariri et al., 2001; Oliveira et al.,1996), DNA vaccines (Munoz et ... vaccines, the search for improved vaccines has never ended. Although the available vaccines are effective in controlling ... In past, inactivated Brucella vaccines have also been tried with limited success (Plommet et al., 1970). The major drawback of ...
Comparative Field Trial Effect of Brucella spp. Vaccines on Seroconversion in Goats and Their Possible Implications to Control ... Brucella spp. is considered as the most common laboratory-acquired pathogens. Several serological tests have been widely used ... Brucella is Gram-negative, nonmotile, coccobacilli or small rods intracellular pathogen that are taxonomically categorized in ... The main reasons behind include the complex nature of Brucella infection, long duration of therapy, and relapse of disease ...
  • in a goat flock and the seroconversion of three groups of animals vaccinated with Rev-1 (Brucella melitensis), RB51, and RB51-SOD (Brucella abortus) to estimate the level of protection conferred on susceptible females. (intechopen.com)
  • Results show that the seroconversion induced by Brucella abortus RB51 and RB51-SOD vaccines is lower than that by Brucella melitensis Rev-1. (intechopen.com)
  • In human populations, the major cause of the disease is Brucella melitensis , but several cases have also been attributed to Brucella abortus , which otherwise primarily affects bovines. (asm.org)
  • In order to evaluate safety of the novel vaccine strains as well as protection against infection and abortion, we tested selected B. melitensis unmarked deletion mutants in a natural host, the goat. (openthesis.org)
  • Brucella melitensis and Brucella abortus are the main causes of human brucellosis, worldwide. (semanticscholar.org)
  • Immunogenicity and protective efficacy of Brucella abortus recombinant protein cocktail (rOmp19+rP39) against B. abortus 544 and B. melitensis 16M infection in murine model. (semanticscholar.org)
  • Accurate typing procedures are critical for eradication and control of disease-causing organisms, but DNA-based techniques are challenging because the various Brucella species and strains have a high degree of genetic homology, as demonstrated by whole-genome comparative analyses of the 3 Brucella genomes sequenced, Brucella melitensis ( 4 ), Brucella suis ( 5 ), and Brucella abortus ( 6 ). (aaccjnls.org)
  • Attenuated Brucella melitensis Rev 1, used as vaccine against ovine and caprine brucellosis caused by B. melitensis , is also considered the best vaccine available for the prophylaxis of B. ovis infection, but its use for this purpose has serious drawbacks. (biomedcentral.com)
  • In this work, two previously characterized B. ovis attenuated mutants (Δ omp25 d and Δ omp22 ) were evaluated in mice, in comparison with B. melitensis Rev 1, as vaccines against B. ovis . (biomedcentral.com)
  • Brucella melitensis Rev 1 is an S live attenuated strain currently used for vaccination campaigns against ovine and caprine brucellosis, which is almost exclusively caused by B. melitensis and B. ovis . (biomedcentral.com)
  • Additionally, due to its serological cross-reactivity with other S Brucella strains, it is banned in countries where B. melitensis has been eradicated, some of which are important sheep producers and where B. ovis infection constitute a serious problem. (biomedcentral.com)
  • Therefore, the development of a new vaccine able to substitute B. melitensis Rev 1 strain in the prophylaxis of B. ovis infection is a matter of great interest. (biomedcentral.com)
  • The live attenuated strains like Brucella melitensis Rev1 and B. abortus S19 and RB51 are being used to control brucellosis in domesticated animals however they have some disadvantages, such as these strains provoke antibodies to their lipopolysaccharide (LPS), making it difficult to distinguish vaccinated animals from those naturally infected. (upm.edu.my)
  • In this study, the outer membrane proteins (Omps) of Brucella melitensis 152, 183 and 293 of local isolates were extracted and characterized using Sodium Dodecyl Sulfate-polyacrylamide Gel Electrophoresis (SDS-PAGE). (upm.edu.my)
  • Immunogenicity evaluation of plasmids encoding Brucella melitensis Omp25 and Omp31 antigens in BALB/c mice. (nih.gov)
  • This study was designed to evaluate the immunogenicity of DNA vaccines encoding B. melitensis outer membrane proteins (Omp25 and Omp31) against B. melitensis Rev1 in a mouse model. (nih.gov)
  • Collectively, these finding suggested that the pcDNA3.1/Hygro DNA vaccines encoding Omp25 and Omp31 genes and divalent plasmid were able to induce both humoral and cellular immunity, and had the potential to be a vaccine candidate for prevention of B. melitensis infections. (nih.gov)
  • Antibody response and antigen-specific gamma-interferon profiles of vaccinated and unvaccinated pregnant sheep experimentally infected with Brucella melitensis . (springer.com)
  • Tulathromycin treatment does not affect bacterial dissemination or clearance of Brucella melitensis 16M following experimental infection of goats. (usda.gov)
  • Mice were immunized orally by administration of B. melitensis WR201, a purine auxotrophic attenuated vaccine strain, then challenged intranasally with B. melitensis 16M. (hindawi.com)
  • The predominant Brucella species in both animal and human infections is Brucella melitensis [ 4 ]. (hindawi.com)
  • A vaccine for B. melitensis for use in humans would be a boon to millions of agriculture workers world-wide [ 5 ] and may be an important goal for protection against bioterrorism [ 6 ]. (hindawi.com)
  • The live B. melitensis Rev.1 strain is considered the best vaccine available for the prophylaxis of brucellosis in small ruminants, especially when used at the standard dose by the conjunctival route. (scielo.br)
  • A brucelose caprina e ovina causada pela espécie Brucella melitensis é uma doença infecciosa que se caracteriza por aborto e esterilidade nas fêmeas e orquite e epipidimite nos machos. (scielo.br)
  • Based on morphological, biochemical characteristics and agglutination test with typo specific sera causes of abortions were identified as Brucella abortus biotypes 6 and Brucella melitensis biotypes 3, results confirmed by the reference laboratory VLA-London. (inovacion.al)
  • We generated a panel of Brucella lipopolysaccharide (LPS)-specific mAbs by immunising mice with inactivated B. melitensis and B. abortus cells. (unibas.ch)
  • Attenuated live vaccines, such as Brucella abortus S19, which until recently was the vaccine used in cattle in the United States, and Brucella melitensis Rev1, which is used in sheep and goats in other parts of the world, have been used successfully in eradication and control programs ( 1 ). (asm.org)
  • Before Brucella melitensis was recognized as the cause of Malta fever in man, a disease causing the same symptoms in countries bordering the Mediterranean was known as Fibris andulans. (au-ibar.org)
  • Brucella melitensis primarily affects the reproductive tract of sheep and goats, and B. melitensis infection is characterized by abortion, retained placenta and, to a lesser extent, impaired fertility. (au-ibar.org)
  • Brucella melitensis is also prevalent in developing countries of South-West Asia, parts of Latin America and Africa where it constitutes a serious human health hazard. (au-ibar.org)
  • The O-polysaccharide of Brucella LPS is critical for virulence of the classical Brucella species ( B. melitensis, B. abortus , B. suis ). (frontiersin.org)
  • Serological and Nucleic Acid Based Detection of Brucellosis in Livestock Species and Molecular Characterization of Brucella melitensis Strains Isolated from Pakistan. (thefreelibrary.com)
  • The blood culture positive bacterial isolations were further subjected to classical biotyping and molecular techniques for characterization and found as non-vaccine strains of Brucella melitensis. (thefreelibrary.com)
  • Recently, Brucella melitensis was declared by the Centers for Disease Control and Prevention to be one of three major bioterrorist agents due to the expense required for the treatment of human brucellosis patients. (asm.org)
  • In fact, the REV.1 vaccine is quite virulent and apparently unstable, creating the need for improved vaccines for B. melitensis. (asm.org)
  • Brucella melitensis (sheep and goats), B. suis (hogs), B. abortus (cattle), B. ovis (sheep), B. canis (dogs), B. neotomae (wood rats), and B. maris (marine mammals) ( 38 , 80 ). (asm.org)
  • The recent completion of B. melitensis (GenBank NC 003317 and NC 003318 ) ( 44 ), B. suis (GenBank NC 002969 ), and B. abortus ( 142 ) genome sequence projects has provided tremendous information for understanding the mechanisms of Brucella pathogenicity. (asm.org)
  • The availability of the genomic sequences of B. melitensis , B. suis , and B. abortus will provide an opportunity to identify the virulence mechanisms of Brucella at the molecular level that have previously proven highly elusive. (asm.org)
  • Hence, the NCBI taxonomy includes most Brucella species under B. melitensis. (wikipedia.org)
  • The aim of this study was to develop a rapid and sensitive immunological assay to detect all Brucellae with 'smooth' LPS, particularly B. melitensis and B. abortus . (biomedcentral.com)
  • Three isolates were identified from 105 blood samples from humans with brucellosis and 50 samples of milk and tissues from infected cows and they were all Brucella melitensis biovar 3. (who.int)
  • pour les trois, le biotype a été déterminé comme étant Brucella melitensis biovar 3. (who.int)
  • This is a bacterial infection caused by a number of types of Brucella bacteria ( Brucella abortus, B. melitensis, B. suis, and B canis ). (sa.gov.au)
  • 2002). ==Genome Structure== The genome of ''Brucella'' ''melitensis'' strain 16M is comprised of 3,294,931 bp in two circular chromosomes. (kenyon.edu)
  • 2002). Like ''B. melitensis, Brucella abortus bivoar'' has two circular chromosomes. (kenyon.edu)
  • As our publications document, we have also developed in silico interactome modeling tools for intracellular bacterial pathogens, Brucella melitensis and Mycobacterium avium ssp. (tamu.edu)
  • Experimental infection of pregnant cattle with the vaccine candidate Brucella abortus strain RB51: pathologic, bacteriologic, and serologic findings. (semanticscholar.org)
  • Brucella abortus is a facultative intracellular bacterium that causes chronic infection in animals and humans. (vt.edu)
  • Comparison of potential protection conferred by three immunization strategies (protein/protein, DNA/DNA, and DNA/protein) against Brucella infection using Omp2b in BALB/c Mice. (semanticscholar.org)
  • Vaccination is an effective strategy for protection against Brucella infection in livestock in developing countries and in wildlife in developed countries. (biomedcentral.com)
  • Therefore, mutant strain ∆22915 could be used as a novel vaccine candidate in the future to protect animals against B. abortus infection. (biomedcentral.com)
  • These characteristics encourage their evaluation in the natural host as homologous vaccines for the specific prophylaxis of B. ovis infection. (biomedcentral.com)
  • On account of the fact that, vaccination is the only way to control and eradicate the disease, the vaccine which can induce antibody in animals and protect them from infection and can overcome the drawbacks of current live attenuated vaccines, is essential. (upm.edu.my)
  • Strain 19 and RB51 are the approved B. abortus vaccines strains most commonly used to protect cattle against infection and abortion. (beds.ac.uk)
  • Brucella ovis infection is usually found in countries with intensive sheep farming. (au-ibar.org)
  • Co-infection with Rift Valley fever virus, Brucella spp. (ilri.org)
  • The identification of host- Brucella interaction is crucial to understand host immunity against Brucella infection and Brucella pathogenesis against host immune responses. (frontiersin.org)
  • Brucellosis is a zoonotic infection caused by Brucella species. (usda.gov)
  • We hypothesize that Stimulation of the innateimmune system together with phage therapy, with or without liposome delivery, willresult in synergistic and successful protection from Brucella infection. (usda.gov)
  • Further, primary Brucella infection was not exacerbated in perforin knockout and Fas-deficient mice and these animals' anti- Brucella immune responses were indistinguishable from those of normal mice. (hindawi.com)
  • In this context, the application of the control / eradication strategy of this infection in cattle from Durres and Lushnja complexes through the use of vaccine with strain Brucella abortus RB51 and evaluation of vaccine immunogenicity through brucelina PPD allergen manufactured by us is the main objective of this study. (inovacion.al)
  • Through the use of this vaccine defined by this study, we obtained the control of Brucella infection in both complexes included in the study and 24 months after the application of the vaccines, complexes are considered to be "free" from brucellosis. (inovacion.al)
  • Brucella inopinata is a Gram-negative, nonmotile, non-spore-forming coccoid bacterium, first isolated from a breast implant infection site. (wikipedia.org)
  • A combined vaccine used to prevent infection with diphtheria and tetanus toxoid. (bioportfolio.com)
  • However, in situ analysis of Brucella infected macrophages using antibody directed against O-polysaccharide suggested a loss of reactivity of Brucella consistent with the appearance of rough organisms, and a potential contribution to infection. (frontiersin.org)
  • Drinking unboiled milk is a risk factor for Brucella infection. (ajtmh.org)
  • may or may not provide cross-protection against infection by heterologous Brucella species, hampering the acceleration of vaccine development. (asm.org)
  • The major objective of the investigation was to evaluate the hitherto uncharacterized potential of Brucella -specific antibodies to win the battle against virulent Brucella abortus infection. (veterinaryworld.org)
  • Therefore, a predominant antibody-mediated response induced by a vaccine is expected to protect the animal against the most severe clinical outcome of infection. (veterinaryworld.org)
  • There is no vaccine to prevent infection in humans. (healthcanal.com)
  • If we can figure out exactly how Brucella accomplishes infection, we could develop an effective drug. (healthcanal.com)
  • Brucella packs a potent punch - only a small dose of 10 to100 bacteria can cause infection. (healthcanal.com)
  • Brucella abortus vaccine strain RB51 infection and exposures associated with raw milk consumption - Wise County, Texas, 2017. (cdc.gov)
  • Brucella , a Gram-negative bacterium, is classified as a potential bioterrorism agent mainly due to the low dose needed to cause infection and the ability to transmit the bacteria via aerosols. (biomedcentral.com)
  • A cross-sectional study using a simple two stage cluster sampling method was conducted to measure short evolution brucellosis burden among cattle keeping households that are one of the highest risk populations to be exposed to Brucella infection. (biomedcentral.com)
  • The goal of this research is to develop a safe and efficacious Brucella canis vaccine using a genetic mutant that has been shown to be safe and efficacious for controlling infection against other Brucella species. (akcchf.org)
  • ABSTRACT A serological study was carried out in Tiaret province in western Algeria on 1032 cows distributed in 95 flocks to estimate the prevalence of Brucella infection and to compare the sensitivity and specificity of a range of agglutination tests. (who.int)
  • Brucella infection is mainly an occupational disease of farm workers, veterinarians and abattoir workers. (sa.gov.au)
  • ABSTRACT Results differ as to whether Brucella infection during pregnancy increases a woman's risk of miscarriage. (who.int)
  • However, it cannot be ascertained whether these antibodies are due to vaccine or response to infection. (veterinaryworld.org)
  • The heightened antibody response after infection may also indicate a secondary immune response to the antigens common to the vaccine strain and wild Brucella organisms. (veterinaryworld.org)
  • Rotavirus Infection, Illness, and Vaccine Performance in Malnourished Children: A Review of the Literature. (amedeo.com)
  • From our very successful and continuing collaboration grew the idea to develop a computational infection biology model based on temporal neonatal calf in vivo RNA-Seq and microarray-based transcriptomic and proteomic profiling of the acute Salmonella, Brucella and Mycobacterium infection process. (tamu.edu)
  • Exposure to the Brucella abortus RB51 culture for confirmation ( 1 ). (cdc.gov)
  • These cases abortus strain RB51 vaccine (RB51) to cattle 3 weeks before highlighted the lack of awareness of the unique challenges in symptom onset. (cdc.gov)
  • OPHD and cians unfamiliar with management of human RB51 Brucella the local health jurisdiction provided RB51-specific treatment infections and exposures. (cdc.gov)
  • Adverse events in humans ity in humans, he had not sought the recommended postexpo- associated with accidental exposure to the livestock brucellosis vaccine RB51. (cdc.gov)
  • The patient reported having sustained a needle stick while administering the Brucella abortus strain RB51 vaccine (RB51) to cattle 3 weeks before symptom onset. (cdc.gov)
  • RB51 is a live-attenuated vaccine, approved for use as part of the Brucellosis Eradication Program in the mid-1990s ( 2 ). (cdc.gov)
  • State and local health jurisdictions should consider regular communication with veterinary and laboratory communities regarding occupational RB51 exposures and can serve as a resource to clinicians unfamiliar with management of human RB51 Brucella infections and exposures. (cdc.gov)
  • Safety Of Brucella Abortus Strain Rb51 Vaccine In Non-Target U" by Terry J. Kreeger, Thomas J. DeLiberto et al. (unl.edu)
  • n =24) were given a single oral dose of at least 1.0X10 10 colony-forming units of Brucella abortus strain RB51 vaccine (RB51). (unl.edu)
  • In order to achieve this objective, vaccine strains of B. abortus S-19 and RB51 ( 19 , 32 ) have been used with relatively good results. (asm.org)
  • To determine the placental tropism and abortigenicity of the vaccine candidate Brucella abortus strain RB51 (SRB51), a rough mutant of the virulent strain 2308, ten Polled Hereford heifers were inoculated intravenously in the 6th month of gestation. (semanticscholar.org)
  • Abortion and premature birth in cattle following vaccination with Brucella abortus strain RB51. (semanticscholar.org)
  • Epidemiological study of Brucellosis in cattle, immunized with Brucella abortus RB51 vaccine in endemic zones. (semanticscholar.org)
  • Efficacy of strain RB51 vaccine in heifers against experimental brucellosis. (semanticscholar.org)
  • B. abortus strain RB51 has been shown to be useful in eliciting protective cell mediated immunity and humoral responses against Brucella in cattle and other animal species. (vt.edu)
  • The vaccine strain B. abortus RB51 was readily distinguished by a specific additional 2524-bp fragment and by the absence of the 1682-bp fragment, and the vaccine strain B. abortus S19 did not produce the 587-bp fragment common to all other Brucella strains tested. (aaccjnls.org)
  • However, current vaccine strains including S19 and RB51 are pathogenic to humans and pregnant animals, limiting their use. (biomedcentral.com)
  • Vaccine strains such as S19, RB51 and Rev.1 have been extensively applied over the past decades with promising effects. (biomedcentral.com)
  • Despite enormous advances and the development of B. abortus S19 and RB51 vaccines, the search for improved vaccines has never ends. (beds.ac.uk)
  • Apart from that, when S19 or RB51 preparations are used in animals, the interference of vaccine induced antibody response with conventional serological tests (Stevens et al. (thefreelibrary.com)
  • Genome report-a genome sequence analysis of the RB51 strain of Brucella abortus in the context of its vaccine properties. (usda.gov)
  • The New York State Department of Health and Pennsylvania Department of Health are investigating Brucella RB51 exposures that may be connected to consuming raw (unpasteurized) milk from Miller's Biodiversity Farm in Quarryville, Pennsylvania. (cdc.gov)
  • This investigation is associated with the third known case of brucellosis from Brucella RB51 due to raw milk acquired in the U.S., since August 2017. (cdc.gov)
  • Milk samples from the dairy tested positive for Brucella strain RB51. (cdc.gov)
  • Brucella strain RB51 is a live-attenuated cattle vaccine strain, which can be shed in milk and can cause infections in humans. (cdc.gov)
  • Comparative behaviour of Brucella abortus strain 19 and RB51 in pregnant mouse. (thefreedictionary.com)
  • An Adolescent With Neurobrucellosis Caused by Brucella abortus Cattle Vaccine Strain RB51. (amedeo.com)
  • This manuscript reviews research on the use of Brucella vaccines in species of wildlife with emphasis on safety and efficacy. (nih.gov)
  • Should this occur, non-target species other than elk and bison may come in contact with the vaccine resulting in morbidity or mortality. (unl.edu)
  • Brucellosis is one of the most common zoonotic diseases caused by species of Brucella. (semanticscholar.org)
  • The genus Brucella consists of 6 recognized bacterial species ( 1 ) and 2 proposed new species recently isolated from marine mammals ( 2 ). (aaccjnls.org)
  • Unique fragments occur among the different Brucella genomes, however, which allowed us to develop a rapid, specific single-test-tube assay for molecular identification of all known Brucella species. (aaccjnls.org)
  • Identification and differentiation of all Brucella species and vaccine strains by multiplex PCR. (aaccjnls.org)
  • After an antigen stimulus with B. ovis cells, splenocytes obtained from all vaccinated mice secreted similar levels of TNF-α and IL12(p40) and remarkably high amounts of IFN-γ, a crucial cytokine in protective immunity against other Brucella species. (biomedcentral.com)
  • However, due to some drawbacks shown by these vaccines much effort has been undertaken for the development of new vaccines, safer and more effective, that could also be used in other susceptible species of animals. (beds.ac.uk)
  • were confirmed as Brucella abortus by PCR using species-specific primers. (usp.br)
  • Brucellosis is a major bacterial zoonoses worldwide (Manish et al, 2013) caused by Brucella species. (thefreelibrary.com)
  • Most of the information about the inter-species interactions between host and Brucella genes is only available in the text of the scientific publications. (frontiersin.org)
  • Next, sentence-level and abstract-level co-occurrence based approaches, as well as sentence-level machine learning based methods, originally designed for extracting intra-species gene interactions, were utilized to extract the interactions among the identified host and Brucella genes. (frontiersin.org)
  • https://doi.org/10.3201/eid1707.110222 sidered atypical Brucella species similar to B. microti and 10. (cdc.gov)
  • To date, the most successful brucellosis vaccine preparations (used in livestock species) have been modified live derivatives of virulent Brucella [ 7 , 8 ]. (hindawi.com)
  • Although new species of Brucella that are potentially zoonotic have been identified with reservoir hosts in wildlife [4-7], the three zoonotic species in domestic livestock are of much greater importance [8]. (symbiosisonlinepublishing.com)
  • Although preferred reservoir hosts for these Brucella species are sheep and goats, swine, and cattle, respectively [8], each has been demonstrated to be capable of infecting other host species. (symbiosisonlinepublishing.com)
  • The mAbs recognised Brucella species with 'smooth' LPS independently of the way how the bacterial cells were inactivated. (unibas.ch)
  • Sequences homologous to the cloned fragment encoding BA14K were identified by Southern blot analysis of genomic DNAs from representatives of all of the currently recognized Brucella species. (asm.org)
  • Lipopolysaccharide-deficient mutants of smooth Brucella species (rough mutants) have been shown to arise spontaneously in culture. (frontiersin.org)
  • On the basis of LPS expression, the classical species can be broken into two broad categories: smooth (S-) or rough (R-). The classification between S- and R-strains is based on the observation of Brucella colonies using the oblique light method ( Henry, 1933 ). (frontiersin.org)
  • Genus Brucella is classified into different species based on phenotypic differences and host preferences. (thefreelibrary.com)
  • 1987). However, since 1994, several new Brucella species have been isolated from marine mammals (Foster et al. (thefreelibrary.com)
  • 2008). More recently, several species of Brucella have been isolated from human (Scholz et al. (thefreelibrary.com)
  • In this study, a custom MALDI-TOF MS reference library was constructed and its performance on identification at species level was evaluated using 75 Brucella spp. (medworm.com)
  • Analysis of mass peak profiles allowed Brucella accurate identification at genus and species level (100%) with no misidentifications. (medworm.com)
  • The different species of Brucella are genetically very similar, although each has a slightly different host specificity. (wikipedia.org)
  • People may also be infected by inhalation of contaminated dust or aerosols, and as such, the CDC has labeled Brucella species as highly weaponizable. (wikipedia.org)
  • The Luminex assay recognised all tested Brucella species with 'smooth' LPS with detection limits of 2 × 10 2 to 8 × 10 4 cells per mL, depending on the species tested. (biomedcentral.com)
  • This is especially important for fastidious agents such as Brucella or Francisella species where tracing by cultivation is hampered by long cultivation time. (biomedcentral.com)
  • Brucella species are small, aerobic, Gram-negative coccobacilli that are animal pathogens of worldwide distribution. (renalandurologynews.com)
  • In addition to this, analyses of three ''Brucella'' species have shown that their genomes lack the functional sequences of so-called classical virulence factors, pathogenic islands, and a complete set of genes to mount, types I, II, and II secretion systems. (kenyon.edu)
  • identify, characterize Brucella strains by Multiple Loci Variable Number of Tandem Repeats Analysis (MLVA-VNTR) and investigate possible spillover to other species. (biomedcentral.com)
  • Four members have recently been classified as additional species [ 10 , 11 ] and recently the eleventh Brucella spp. (biomedcentral.com)
  • Taken together, each of Brucella antigens delivered by ST successfully induced antigen specific immune response and it was also evident that individual antigen strain can also confer considerable degree of protection. (vetsci.org)
  • In this study, we constructed the Brucella abortus ( B. abortus ) S2308 mutant strain Δ22915, in which the putative lytic transglycosylase gene BAB_RS22915 was deleted. (biomedcentral.com)
  • Immune responses and clinical effects of experimental challenge of elk with Brucella abortus strain 2308. (usda.gov)
  • Brucella suis strain 353-1 is a stable vaccine strain that is clinically safe, does not cause positive serologic responses on conventional brucellosis surveillance tests, and induces humoral and cellular immunity in swine after vaccination. (symbiosisonlinepublishing.com)
  • Feral swine vaccinated orally or parentally with strain 353-1 had greater (P Brucella at all sampling times after vaccination when compared to non-vaccinated swine. (symbiosisonlinepublishing.com)
  • This paper aimed to determine the excretion period of B19 vaccine strain during a complete reproductive cycle (from estrus synchronization , artificial insemination , pregnancy and until 30 days after parturition ) of dairy cows from 3 to 9 years old that were previously vaccinated from 3 to 8 months. (bvsalud.org)
  • Furthermore, distinct peak biomarkers were detected for biovar assignment and discrimination from vaccine strain Rev.1. (medworm.com)
  • Bacillus Calmette-Guérin (BCG) is an attenuated strain of M. bovis , which is widely used around the world as a vaccine against human TB caused by Mycobacterium tuberculosis . (royalsocietypublishing.org)
  • Vaccines may be effective against one particular strain of an agent but not against a different strain. (missouri.edu)
  • Additionally, conditions required for an effective Brucella vaccine strain as well as the future research direction needed to investigate Brucella pathogenesis and host immunity are postulated. (asm.org)
  • Verma S, Rawat M, Kumawat S, Qureshi S, Mohd G, Tiwari AK (2018) Protective role of Brucella abortus specific murine antibodies in inhibiting systemic proliferation of virulent strain 544 in mice and guinea pig, Veterinary World, 11(6): 794-799. (veterinaryworld.org)
  • a stable rough strain of Brucella abortus . (cdc.gov)
  • naturally occurring mutant, low-virulence strain of Brucella abortus . (thefreedictionary.com)
  • But the problem with using strain 19 in a vaccine has always been that it induced an antibody response, making identification of truly infected animals more difficult," says ARS veterinarian Steven C. (thefreedictionary.com)
  • It's because the most common vaccine, Strain 19 , uses a live strain of the brucellosis bacterium, Brucella abortus. (thefreedictionary.com)
  • abortus vaccine strain 19 in hopes of deleting a specific protein immunogen. (thefreedictionary.com)
  • At the beginning of this century in the US, Brucella abortus is a problem in elk and bison in the Greater Yellowstone Area, B. suis is prevalent in millions of feral swine in most of the southern states, and caribou/reindeer in Alaska are infected with B. suis biovar 4. (nih.gov)
  • Loci associated with antibody response in feral swine (Sus scrofa)infected with Brucella suis. (usda.gov)
  • 2002). ''Brucella'' ''suis'' was the first pathogenic organism used by the U.S. military as a weapon backing in the 1950s. (kenyon.edu)
  • A simple, rapid, inexpensive fluorescence polarization assay for the detection of antibodies to Brucella abortus in bulk tank milk samples at the farm level or at dairies with a sensitivity and specificity of 100 and 95.9%, respectively, is described. (asm.org)
  • Animals vaccinated with pcDNA-SOD did not develop SOD-specific antibodies, at least until week 4 after immunization (the end of the experiment), and in vitro stimulation of their splenocytes with either recombinant Cu-Zn SOD or crude Brucella protein induced the secretion of gamma interferon (IFN-γ), but not interleukin-4, and elicited the induction of cytotoxic-T-lymphocyte activity. (asm.org)
  • To fulfill the need for a simple and rapid diagnostic test for human brucellosis, we used the immunochromatographic lateral flow assay format to develop two assays, one for the detection of Brucella -specific immunoglobulin M (IgM) antibodies and one for the detection of Brucella -specific IgG antibodies. (asm.org)
  • To address the need for a simple and rapid diagnostic test, we have applied the immunochromatographic lateral flow assay format to develop two assays, one for the detection of Brucella -specific IgM and one for Brucella -specific IgG antibodies. (asm.org)
  • Since the protective antigen (PA) of B. anthracis is known to induce protective antibodies, it was decided that the objective of this research was to test whether the gene encoding PA could be expressed in Brucella producing a bivalent vaccine to protect against both brucellosis and anthrax. (vt.edu)
  • Evaluation of a fluorescence polarization assay for the detection of serum antibodies to Brucella abortus in water buffalo ( Bubalus bubalis ). (springer.com)
  • In particular, our strategy was to produce Brucella cell surface antigen-specific monoclonal antibodies (mAbs) for the development of an antigen capture assay for the detection of Brucella cells as potential bio threat agents in complex samples. (unibas.ch)
  • Western blot analyses with Brucella lysate also identified antibodies against some immunodominant Brucella proteins in the serum of cattle naturally infected with Brucella spp. (unibas.ch)
  • A low-molecular-weight recombinant Brucella abortus protein reactive with antibodies from a variety of naturally and experimentally infected hosts and T lymphocytes from experimentally infected mice was identified and given the designation BA14K. (asm.org)
  • The potential of DNA vaccines to act in presence of maternal antibodies, its stability and cost effectiveness and the non-requirement of cold chain have heightened the prospects. (springer.com)
  • To this end, monoclonal antibodies (mAbs) specific for Brucella LPS were generated and used to design a highly specific and sensitive antigen capture assay. (biomedcentral.com)
  • Household blood samples were tested for Brucella antibodies using the highly sensitive Rose Bengal test (RBT) and IgM ELISA Lateral Flow Assay (LFA). (biomedcentral.com)
  • most importantly, it is caused by bacteria of the genus Brucella [ 1 ]. (intechopen.com)
  • The disease results from facultative intracellular bacteria belonging to the genus Brucella which are Gram-negative, nonspore-forming and noncapsulated coccobacilli [ 3 , 5 , 6 ]. (hindawi.com)
  • Brucellosis is a zoonosis of global socio-economic importance caused by Gram negative facultative intracellular bacteria of genus Brucella. (thefreelibrary.com)
  • Brucellosis is one of the five common bacterial zoonoses in the world caused by organisms belonging to the genus Brucella , gram-negative, non-spore-forming, facultative, intracellular bacteria ( 39 ). (asm.org)
  • Brucella is a genus of Gram-negative bacteria, named after David Bruce (1855-1931). (wikipedia.org)
  • In this study, a clinical assay was performed to establish the efficacy of this vaccine on double purpose cattle. (scialert.net)
  • Therefore, the objective of this study was to evaluate Brucella abortus S19 vaccine in cattle naturally infected with brucellosis under tropical conditions. (scialert.net)
  • However, even these vaccine strains are far from ideal, since they present some disadvantages, e.g., causing reactions in humans, inducing abortion in pregnant cattle, and showing a likelihood of changing to a virulent form ( 33 ). (asm.org)
  • Enhancing the detection of Brucella-specific CD4+ T cell responses in cattle via in vitro antigenic expansion and restimulation. (usda.gov)
  • Brucella abortus vaccine is used for the immunization of cattle, sheep, and goats. (bioportfolio.com)
  • As the aim of a badger vaccine would be to at least limit transmission from infected badger populations to cattle, we reasoned that a vaccine which prevented disease progression to a point before animals became infectious could still achieve this goal [ 18 ]. (royalsocietypublishing.org)
  • The use of vaccines to control brucellosis in populations of wildlife and therefore reducing the risk of transmission to humans and livestock has been proposed in several instances. (nih.gov)
  • Although he administered the vaccine regularly and was aware of its potential for pathogenicity in humans, he had not sought the recommended postexposure prophylaxis of doxycycline and TMX/SMX for 21 days ( 1 ). (cdc.gov)
  • Commercially available anthrax vaccines for immunization of humans are of limited duration and do not protect against the respiratory form of the disease. (vt.edu)
  • Brucellosis is a widespread disease affecting livestock and humans caused by Brucella spp. (upm.edu.my)
  • Live attenuated Brucella vaccines are considered effective in animals but they may be potentially infectious to humans, so it is vital to improve the immunoprotective effects and safety of vaccines against Brucella. (nih.gov)
  • Although the available vaccines are effective in controlling brucellosis, they have numerous drawbacks, such as interference with diagnostic tests, pathogenicity for humans, potential to cause abortion in pregnant animals, among others. (beds.ac.uk)
  • Brucella is an intracellular bacterium that causes chronic brucellosis in humans and various mammals. (frontiersin.org)
  • Brucella is a Gram-negative intracellular bacterium that causes zoonotic brucellosis in humans and various animals. (frontiersin.org)
  • Atypical Brucella lesions in humans, an outbreak of glanders in Bahrain suggests multiple wild mammals, amphibians, and fish range from lo- introduction events. (cdc.gov)
  • In general, B. canis appears to cause less severe clinical symptoms in humans than other Brucella spp. (cdc.gov)
  • However, some of these vaccines are pathogenic in humans [ 9 ]. (hindawi.com)
  • After exposure to Brucella, humans generally have a two- to four-week latency period before exhibiting symptoms, which include acute undulating fever (>90% of all cases), headache, arthralgia (>50%), night sweats, fatigue, and anorexia. (wikipedia.org)
  • Unlike other bacterium, Brucella is able to sneak like a spy into the body's immune cells, causing abortions and sterility in animals and a debilitating flu-like disease in humans. (healthcanal.com)
  • Brucellosis, a zoonotic bacterial disease caused by Gram-negative Brucellae and classified as a potential bioterrorism disease [ 1 ], leads to abortions in animals and flu-like symptoms with periodic bouts of fever in humans. (biomedcentral.com)
  • Brucella canis had not been isolated in the Netherlands until November 2016, when it was isolated from a dog imported from Romania. (cdc.gov)
  • The aim of this study was to estimate the diversity and prevalence of both groups of Brucella canis 1 and 2 with and without deletion respectively in different areas of Argentina. (elsevier.es)
  • Canine brucellosis is a reproductive disease caused by the bacterium Brucella cani s ( B. canis ), which can cause infertility, abortion, and severe spinal infections in dogs. (akcchf.org)
  • The development of such a vaccine for dogs will significantly impact canine and human health by limiting the spread of B. canis . (akcchf.org)
  • Update: ACIP Recommendations for the Use of Quadrivalent Live Attenuated Influenza Vaccine (LAIV4) - United States, 2018-19 Influenza Season. (bioportfolio.com)
  • Brucella ceti is a gram negative bacterial pathogen of the Brucellaceae family that causes brucellosis in cetaceans. (wikipedia.org)
  • Only a small portion of those with Brucella ceti have overt clinical signs of brucellosis indicating that many have the bacteria and remain asymptomatic or overcome the pathogen. (wikipedia.org)
  • Although Brucella is a serious pathogen, type I, II, and III secretion systems and pathogenicity islands were absent. (asm.org)
  • In Marseille, France, his research is centered on combating Infectious diseases by studying host/pathogen interactions and vaccine design. (elsevier.com)
  • Here, with the support of a recently awarded $458,000 National Institutes of Health grant, Caswell studies the elusive activities of a bacterium called Brucella. (healthcanal.com)
  • With this latest grant, we will examine the unique genetic circuitry of Brucella, and how it allows the bacterium to trick, invade, and live inside immune cells," said Caswell, an assistant professor of bacteriology in the Virginia-Maryland College of Veterinary Medicine and a Fralin Life Science Institute affiliate. (healthcanal.com)
  • Six groups of BALB/c mice (seven mice per group) were intramuscularly injected with three recombinant constructs, native pcDNA3.1/Hygro (+) and phosphate-buffered saline (PBS) as controls and subcutaneous injection of attenuated live vaccine Rev1. (nih.gov)
  • Several new generation vaccines like recombinant vaccine (Al-Mariri et al. (thefreelibrary.com)
  • Studies employing both purified subunit preparations and live, recombinant antigen delivery systems should allow a comprehensive evaluation of the relative importance of specific Brucella proteins in eliciting protective immunity. (asm.org)
  • In an attempt to identify Brucella proteins capable of inducing protective immune responses, a collection of recombinant Escherichia coli clones expressing Brucella proteins reactive in immunoassays with sera from a variety of experimentally and naturally infected hosts was assembled ( 18 ). (asm.org)
  • One of these clones, which was designated IV-4, produced a recombinant Brucella protein with an apparent molecular mass of 14 kDa as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis. (asm.org)
  • 1999. Oral vaccination of white-tailed deer using a recombinant bacillus Calmette-Guerin vaccine expressing the Borrelia burgdorferi outer surface protein A: prospects for immunocontraception. (usda.gov)
  • The use of lytic phages to inactivate bacteria and the application of the phage lysate as a vaccine antigen is an alternate approach to develop candidate vaccine against bacterial disease but it has been poorly explored. (thefreelibrary.com)
  • The profound capacity of TLRs mediated immunity can be exploited to augment the antigen specific immune response for many of the vaccine antigens. (thefreelibrary.com)
  • Bovine immune response to leptospiral antigen in different novel adjuvants and vaccine delivery platforms. (usda.gov)
  • Brucella outer membrane protein 2b (Omp2b) is a 36 kD porin existed in common Brucella pathogens and it is considered as priority antigen for designing a new subunit vaccine. (bvsalud.org)
  • In a second part of the thesis we aimed at evaluating the potential of the Plasmodium falciparum reticulocyte-binding homolog 2 (PfRH2) present in the rhoptries as a malaria blood stage vaccine antigen. (unibas.ch)
  • The cloning and characterization of genes encoding immunoreactive Brucella proteins will provide a useful source of antigen for immunologic assays and subunit immunization studies. (asm.org)
  • Biological data suggest that increased vaccine antigen exposure could increase the risk f. (bioportfolio.com)
  • MAbs specific for the LPS O-antigen of Brucella spp . (biomedcentral.com)
  • Each vector constitutively express highly conserved Brucella antigens (rB), viz. (vetsci.org)
  • We aimed at identifying immunodominant Brucella antigens for implementation in new detection tools or for subunit vaccine development. (unibas.ch)
  • It is possible that an oral brucellosis vaccine could be developed and disseminated in the GYA to reduce disease transmission. (unl.edu)
  • formed basis of brucellosis vaccine. (usda.gov)
  • Although bacterial isolation was used as reference, Brucella spp. (usp.br)
  • Live Bacterial Vectors-A Promising DNA Vaccine Delivery System. (bioportfolio.com)
  • A bacterial vaccine for the prevention of brucellosis in man and animal. (bioportfolio.com)
  • Currently, these investigators ( 44 ) are evaluating the proteomic expression of Brucella to identify the bacterial proteins expressed in vitro. (asm.org)
  • As a specialist of bacterial pathogenesis, he is the one who characterized the replication niche of Brucella and also identified host partners of Salmonella effectors secreted by the type III secretion system. (elsevier.com)
  • Additionally, the Luminex assay works in a multiplex format, simultaneously detecting four category A and B bacterial bioterrorism agents and is suitable for detecting Brucella in complex samples. (biomedcentral.com)
  • In the development of vaccines capable of providing immunity against brucellosis, Cu-Zn superoxide dismutase (SOD) has been demonstrated to be one of the protective immunogens of Brucella abortus . (asm.org)
  • On the other hand, DNA vaccines have many advantages over traditional protein-based vaccines, such as ease of development, inducing a long lived immunity, and minimal preparation costs. (upm.edu.my)
  • In the recent years, there has much emphasis on improvement of vaccine utilizing better adjuvant targeting innate immune response to enhance both humoral and cell-mediated immunity. (thefreelibrary.com)
  • Antibody to the O-polysaccharide of Brucella LPS has been firmly established as an important mediator of anti- Brucella effects in murine models of secondary immunity [ 13 , 14 ]. (hindawi.com)
  • Similarly to other infections caused by facultative intracellular pathogens, the induction of specific cell-mediated immunity is required for effective clearance of Brucella infections ( 4 , 22 ). (asm.org)
  • This vaccine induces long-lasting intestinal and humoral immunity. (bioportfolio.com)
  • Killed vaccine induces only humoral immunity. (bioportfolio.com)
  • This review provides our current understanding of Brucella pathogenesis and host immunity for the development of genetically defined efficient vaccine strains. (asm.org)
  • Persistence of Immunity Following 2-Dose Priming with a 10-Valent Pneumococcal Conjugate Vaccine at 6 and 10 Weeks or 6 and 14 Weeks of Age in Nepalese Toddlers. (amedeo.com)
  • Factors That Influence Infant Immunity and Vaccine Responses. (amedeo.com)
  • At present, there is no commercially available vaccine for the human brucellosis. (semanticscholar.org)
  • The RBL supports basic research to develop drugs, diagnostics, and vaccines for emerging and reemerging infections and biodefense. (nih.gov)
  • This translates to study and innovation in medical diagnostic systems and bioassays, medical implant designs and improvement using innovative biomaterials and on-baord drug delivery, implant-related infections, surface analysis in biomedical systems, drug delivery systems for proteins and vaccines, and novel surface modification strategies. (utah.edu)
  • In pregnant patients, Brucella infections can be associated with miscarriage. (cdc.gov)
  • Brucella infections constitute a serious problem for dog breeders, pet owners, and kennels. (akcchf.org)
  • Authors: Zaitoon H, Bamberger E, Yaniv L, Mendelson B, Srugo I, Chistyakov I Abstract BACKGROUND: The introduction of pneumococcal conjugate vaccine-13 (PCV-13) has reduced the burden of invasive pneumococcal disease. (medworm.com)
  • To describe the safety of the 2016-2017 formulation of Fluzone Quadrivalent vaccine in children 3 to (bioportfolio.com)
  • The aim of the study is to evaluate the safety and immunogenicity of the 2015-2016 formulations of Fluzone Quadrivalent and Fluzone Intradermal Quadrivalent vaccines in adults 18 to (bioportfolio.com)
  • The study will evaluate the safety and immunogenicity of the 2015-2016 formulation of Fluzone Quadrivalent vaccine, administered in a 1- or 2-dose schedule, in accordance with the Advisory. (bioportfolio.com)
  • Quadrivalent Meningococcal Vaccine Uptake Among Men Who Have Sex With Men During a Meningococcal Outbreak in Los Angeles County, California, 2016-2017. (bioportfolio.com)
  • 7. Goodwin, Z.I. and Pascual, D.W. (2016) Brucellosis vaccines for livestock. (veterinaryworld.org)
  • This study will evaluate whether it is possible to generate baseline biomarker-based prediction rules (PdR) for vaccine response (post baseline absolute serum antibody titer) using each of. (bioportfolio.com)
  • Our aim was to specifically detect Brucellae with 'smooth' lipopolysaccharide (LPS) using a highly sensitive monoclonal antibody (mAb) based immunological assay. (biomedcentral.com)
  • Despite being perhaps less efficacious, subunit vaccines may have safety advantages over live attenuated candidates. (hindawi.com)
  • Delineation of the immune mechanisms responsible for vaccine-induced protection may focus subunit vaccine development by suggesting potential immune correlates and adjuvants tailored to evoke a desired response. (hindawi.com)
  • Our results indicate that Omp2b protein has a potential to induce both B-cell- and T-cell-mediated immune responses and it can be evaluated as a new subunit vaccine candidate against brucellosis. (bvsalud.org)
  • Brucella bacteria are relatively slow growing, and the culture result may not become available for several days or weeks ( 25 ). (asm.org)
  • We report here that the LPS from the facultative intracellular Gram-negative bacteria Brucella abortus interferes with the MHC class II Ag presentation pathway. (jimmunol.org)
  • Descubrimos que algunas enzimas del metabolismo de flavinas constituyen factores de virulencia que actúan en el nicho replicativo de la bacteria, y que Brucella tiene un metabolismo de síntesis de flavinas atípico. (leloir.org.ar)
  • Estudiamos además una flavoproteína de Brucella, LOV-HK, que es capaz de sensar la luz azul y de esa manera modula la virulencia de la bacteria. (leloir.org.ar)
  • The Brucella genome consists of two circular chromosomes without plasmids, suggesting a remarkable difference compared to the single chromosome of many bacteria ( 105 , 106 ). (asm.org)
  • Like other Gram-negative bacteria, Brucellae express lipopolysaccharide (LPS), a major component of the outer membrane. (biomedcentral.com)
  • Diagnosis is made by growing Brucella bacteria from the blood, bone marrow or other infected body tissues or from discharges from infected body tissues. (sa.gov.au)
  • Brucella are small (0.5 to 0.7 by 0.6 to 1.5 μm), gram negative, non-motile, non- encapsulated, non-spore forming, rod shaped (coccobacilli) bacteria which are facultative intracellular parasites. (biomedcentral.com)
  • Additionally, modified live vaccines may induce abortion to livestock if administered during pregnancy or to other animals in contact with pregnant animals [ 10 , 11 ]. (hindawi.com)
  • The need for a livestock vaccine program is essential to the overall health of the animals. (allivet.com)
  • Livestock vaccines help control the spread of dangerous and contagious diseases. (allivet.com)
  • Different livestock vaccines protect against bovine papilloma virus, foot rot, liver abscesses, bovine pinkeye and blackleg and many others. (allivet.com)
  • Improved immunogenicity and protective efficacy of a divalent DNA vaccine encoding Brucella L7/L12-truncated Omp31 fusion protein by a DNA priming and protein boosting regimen. (semanticscholar.org)
  • Deletion of these genes reduces Brucella virulence, but they maintain excellent immunogenicity to activate the host immune response. (biomedcentral.com)
  • This multicenter, open-label, Phase IV study was designed to describe the safety and immunogenicity of Fluzone Quadrivalent vaccine in children 6 months to (bioportfolio.com)
  • Immunogenicity and Safety of a Tetravalent Dengue Vaccine Administered Concomitantly or Sequentially With Quadrivalent Human Papillomavirus Vaccine in Boys and Girls 9-13 Years of Age in Malaysia: A Phase IIIb, Randomized, Open-label Study. (amedeo.com)
  • Immunogenicity and Safety of AS03-adjuvanted H5N1 Influenza Vaccine in Children 6-35 Months of Age: Results From a Phase 2, Randomized, Observer-blind, Multicenter, Dose-ranging Study. (amedeo.com)
  • Immunogenicity and Safety of a Tetravalent Dengue Vaccine Administered Concomitantly or Sequentially With Tdap Vaccine: Randomized Phase IIIb Trial in Healthy Participants 9-60 Years of Age in the Philippines. (amedeo.com)
  • Brucella ovis causes ram contagious epididymitis, a disease for which a specific vaccine is lacking. (biomedcentral.com)
  • Brucellosis caused by Brucella ovis mainly induces chronic epididymitis and orchitis in rams. (biomedcentral.com)
  • Simmons and Hall, 1953), and the organism was named Brucella ovis in 1956 (Buddel, 1956). (au-ibar.org)
  • These mutants provide protection against wild-type, virulent Brucella challenge in mouse models [ 13 - 17 ], making them potential vaccine candidates. (biomedcentral.com)
  • The production of IFN- γ is essential for protection, clearance, and survival in the face of virulent Brucella challenge in the mouse model [ 17 ]. (hindawi.com)
  • therefore, it is necessary to know the efficacy of this vaccine under different field conditions. (scialert.net)
  • DNA vaccine encoding molecular chaperone GroEL of Edwardsiella tarda confers protective efficacy against edwardsiellosis. (semanticscholar.org)
  • This review highlights the salient features of DNA vaccines, and measures to enhance their efficacy so as to devise an effective and novel vaccination strategy against animal diseases. (springer.com)
  • Brucella abortus vaccines play a central role in bovine brucellosis control/eradication programs and have been successfully used worldwide for decades. (beds.ac.uk)
  • In this paper, we present a review of the main aspects of the vaccines that have been used in the bovine brucellosis control and eradication over the years and some of the current advances in the research for a new B. abortus vaccine. (beds.ac.uk)
  • The asp24 mutants, which persist for extended periods in vivo, appear superior as a vaccine candidate compared to approved vaccine strains S19 and Rev1 in the mouse model against either homologous or heterologous challenges. (openthesis.org)
  • Thank you for sharing this Clinical and Vaccine Immunology article. (asm.org)
  • Message Body (Your Name) thought you would be interested in this article in Clinical and Vaccine Immunology. (asm.org)
  • His training in Brucella research started in 1996 in Dr. Gerhardt Schurig's immunology laboratory at the Virginia Polytechnic Institute and State University (Virginia Tech). He obtained his Ph.D. in Dr. Schurig\\\\\\\'s lab in 2000. (omicsonline.org)
  • Site-directed, unmarked deletion is an effective method for identifying virulence genes and constructing attenuated strains as Brucella vaccines. (biomedcentral.com)
  • 2017 Market Research Report on Brucella Abortus Vaccines Industry was a professional and depth research report on Brucella Abortus Vaccines industry that you would know the world's major regional market conditions of Brucella Abortus Vaccines industry, the main region including North American, Europe and Asia etc., and the main country including United States ,Germany ,Japan and China etc. (reportsweb.com)
  • and foot and mouth disease, Aujesky's disease, swine fever, rabies, canine distemper and brucellosis in animals, DNA vaccine clinical trials are underway. (springer.com)
  • A PCR assay using primers designed based on the gene that encodes a 31 KDa immunogenic protein from Brucella abortus was used in the detection of Brucella spp. (usp.br)
  • The SciMiner tool, originally designed for detecting mammalian gene/protein names in text, was extended to identify host and Brucella gene/protein names in the abstracts. (frontiersin.org)
  • In the current study, we aimed to predict and analyze the secondary and tertiary structures of the Brucella abortus Omp2b protein, and to predict T-cell and B-cell epitopes with the help of bioinformatics tools. (bvsalud.org)
  • Inoculation of plasmid DNA, encoding an immunogenic protein gene of an infectious agent, stands out as a novel approach for developing new generation vaccines for prevention of infectious diseases of animals. (springer.com)
  • Also, the induction of immune responses by DNA vaccines is inconclusive due to the lack of knowledge regarding the concentration of the protein expressed in vivo . (springer.com)
  • Urine and milk samples were submitted to bacteriological culture and urine and PCR reactions for detection of Brucella spp. (bvsalud.org)
  • Integration of the Luminex assay into a multiplex format enabled simultaneous detection of Brucella spp. (unibas.ch)
  • The developed Luminex assay may be applied for the detection of whole Brucella cells both in natural Brucella outbreak and in bioterrorism attack scenarios. (unibas.ch)
  • Detection of rotavirus before and after monovalent rotavirus vaccine introduction and vaccine effectiveness among children in mainland Tanzania. (bioportfolio.com)
  • This study aimed at molecular detection and characterization of Brucella spp from Pakistan. (thefreelibrary.com)
  • Molecular detection of Brucella spp. (academicjournals.org)
  • Based on these results, we conclude that i.s. immunization with pcDNA-SOD vaccine efficiently induced a Th1 type of immune response and a protective response that could be related to IFN-γ production and cytotoxic activity against infected cells by SOD-specific CD4 + and CD8 + T cells, respectively. (asm.org)
  • However, is expected that vaccination against Brucella should elicit a CTL response, since it is related to the development of a Th1-type immune response ( 16 ). (asm.org)
  • Historical data suggests that prolonged survival of Brucella vaccine organisms in the target host enhances immune protection. (openthesis.org)
  • Similarities, but also significant differences, were found regarding the immune response induced by the three vaccines. (biomedcentral.com)
  • We propose to use Brucella-specific phages, in combination withinnate immune stimulation, as a novel countermeasure for brucellosis. (usda.gov)
  • The purpose of this study is to determine the effects of an HIV vaccine (Remune) on the immune system. (bioportfolio.com)
  • This study will test the safety of and immune system response to a new HIV vaccine. (bioportfolio.com)
  • Some parents are concerned that multiple vaccines in early childhood could weaken their child's immune system. (bioportfolio.com)
  • This can include administration of immunopotentiating agents such as BCG vaccine and Corynebacterium parvum as well as biological response modifiers such as interferons, interleukins, and colony-stimulating factors in order to directly stimulate the immune system. (bioportfolio.com)
  • Induction of immune responses by DNA vaccines in large animals. (springer.com)
  • Brucella -specific immune serum was raised in mice. (veterinaryworld.org)
  • We hypothesize that in order for a vaccine to be efficacious, it must survive in the host. (openthesis.org)
  • The development of an efficacious vaccine for brucellosis control/eradication has been a challenge for scientists for many years. (beds.ac.uk)
  • An anti- Brucella vaccine candidate which comprises of purified Brucella lipopolysaccharide and cocktail of four Salmonella Typhimurium (ST)- Brucella vectors was reported earlier. (vetsci.org)
  • Brucella strains are smooth (S) or rough (R), depending on the presence or absence of O-polysaccharide (O-PS) chains in the lipopolysaccharide (LPS) respectively. (biomedcentral.com)
  • Please see the AHPC library for further information on this disease from OIE, including the International Animal Health Code and the Manual of Standards for Diagnostic Tests and Vaccines. (au-ibar.org)
  • In: Manual of diagnostic tests and vaccines for terrestrial animals. (cdc.gov)
  • This is an essential step for the design of new diagnostic tests and vaccines. (healthcanal.com)
  • Confirmation of infected herd was carried out by isolation and identification of Brucella abortus colonies and PCR on milk samples from RT reactor animals. (scialert.net)
  • Specimens obtained from referred aborted sheep and goat fetuses were cultured on Brucella selective media for microbiological isolation. (hindawi.com)
  • Most of the data is based on sero-epidemiological studies although it is generally accepted that only the isolation of Brucella confirms the presence of brucellosis. (au-ibar.org)
  • The aim of this study was to determine the seroprevalence of Brucella spp. (intechopen.com)
  • We determined the seroprevalence of Brucella spp. (who.int)
  • The exact mechanism for protective ability of this candidate vaccine has been poorly understood. (thefreelibrary.com)
  • Therefore, the identification of Brucella cellular components which contribute to the induction of protective responses in the host will be an important step in designing improved vaccines. (asm.org)
  • Total B. abortus S544 counts in the spleen of each animal collected on the 7th day of challenge was determined to evaluate the protective index (PI) of anti- Brucella serum by statistical analysis. (veterinaryworld.org)
  • A live vaccine containing attenuated poliovirus, types I, II, and III, grown in monkey kidney cell tissue culture, used for routine immunization of children against polio. (bioportfolio.com)
  • Active immunization where vaccine is administered for therapeutic or preventive purposes. (bioportfolio.com)
  • The Advisory Committee on Immunization Practices (ACIP) recommends routine vaccination of persons aged 11-12 years with human papillomavirus (HPV) vaccine, quadrivalent meningococcal conjugate vaccine. (bioportfolio.com)
  • A Retrospective Study of the Impact of Pneumococcal Conjugate Vaccine-13 Immunization in a Northern Israel Hospital. (medworm.com)
  • Effectiveness of Rose Bengal test and fluorescence polarization assay in the diagnosis of Brucella spp. (springer.com)
  • Two mAbs were implemented into a bead-based Luminex assay detecting 'smooth' Brucella spp. (unibas.ch)
  • A specific additional 1320-bp fragment was amplified, and the 450-bp fragment was absent in Brucella strains isolated from marine mammals. (aaccjnls.org)
  • In an effort to develop improved vaccine strains, we have screened signature tagged mutagenesis banks to identify mutants with varying survival characteristics. (openthesis.org)
  • Genetic trans-complementation using a plasmid-based expression of Brucella manBA successfully restored O-polysaccharide expression in only one-third of O-polysaccharide deficient spontaneous mutants. (frontiersin.org)
  • We also tried to generate mAbs against Brucella cell surface proteins from mice immunised with inactivated whole Brucella cells. (unibas.ch)
  • however, identification of the recognised proteins with a Brucella-specific peptide microarray failed. (unibas.ch)
  • Future studies to define the role of these secretion systems in Brucella intracellular survival will be necessary before we can understand the mechanisms of Brucella pathogenesis. (asm.org)
  • Although the available vaccines are effective in controlling brucellosis, they have numerous drawbacks like abortifacient potential for pregnant animals (Smith and Ficht, 1990), residual virulence ability for human (Spink et al. (thefreelibrary.com)
  • 1970). The major drawback of chemically killed vaccines include high production cost, poor protection, residual virulence, batch to batch variation, denaturation of conformational epitopes, unaccepted local reaction, serological problems (Moriyon et al. (thefreelibrary.com)
  • Such analyses are subject to variable interpretations and must be performed by skilled technicians who are at high risk because most clinical Brucella strains are highly pathogenic. (aaccjnls.org)
  • Together these characteristics indicate that the Brucella IgM and IgG flow assays are ideal for use in clinical settings in rural and suburban areas in which brucellosis is endemic. (asm.org)
  • 1996), DNA vaccines (Munoz et al, 2004), genetically modified vaccines, vectored vaccines (Ruecker and Guzman, 2012) have been tried with varying degree of success but none showed the results equivalent to the Brucella S19 vaccine (Schurig et al. (thefreelibrary.com)
  • For decades, a live vaccine has been successfully used in risk groups but is presently not available due to difficulties in standardisation. (ersjournals.com)
  • Results indicated that DNA vaccine immunized BALB/c mice had a dominant immunoglobulin G response and elicited a T-cell-proliferative response and induced significant levels of interferon gamma (INF-γ) compared to the control groups. (nih.gov)
  • The experiments reported describe the direct recovery of Brucella from macrophages infected in vitro and from the spleens of infected mice at a frequency similar to that described in vitro , suggesting that Brucella dissociation is not simply an in vitro artifact. (frontiersin.org)