A water-soluble, enzyme co-factor present in minute amounts in every living cell. It occurs mainly bound to proteins or polypeptides and is abundant in liver, kidney, pancreas, yeast, and milk.
A species of the genus BRUCELLA which are pathogenic to SHEEP.
A biotin-dependent enzyme belonging to the ligase family that catalyzes the addition of CARBON DIOXIDE to pyruvate. It is occurs in both plants and animals. Deficiency of this enzyme causes severe psychomotor retardation and ACIDOSIS, LACTIC in infants. EC 6.4.1.1.
A group of compounds that are derivatives of heptanedioic acid with the general formula R-C7H11O4.
Enzymes that catalyze the joining of two molecules by the formation of a carbon-nitrogen bond. EC 6.3.
A genus of gram-negative, aerobic bacteria that causes BRUCELLOSIS. Its cells are nonmotile coccobacilli and are animal parasites and pathogens. The bacterium is transmissible to humans through contact with infected dairy products or tissue.
A carboxylating enzyme that catalyzes the conversion of ATP, acetyl-CoA, and HCO3- to ADP, orthophosphate, and malonyl-CoA. It is a biotinyl-protein that also catalyzes transcarboxylation. The plant enzyme also carboxylates propanoyl-CoA and butanoyl-CoA (From Enzyme Nomenclature, 1992) EC 6.4.1.2.
A primitive form of digestive gland found in marine ARTHROPODS, that contains cells similar to those found in the mammalian liver (HEPATOCYTES), and the PANCREAS.
Formal procedures whereby the employee expresses any dissatisfaction or feeling of injustice regarding the work situation.
A system for the collection and/or processing of data from various sources, and using the information for policy making and management of health services. It could be paper-based or electronic. (From http://web.worldbank.org/WBSITE/EXTERNAL/TOPICS/EXTHEALTHNUTRITIONANDPOPULATION/EXTHSD/0,,contentMDK:22239824~menuPK:376799~pagePK:148956~piPK:216618~theSitePK:376793,00.html. http://www.who.int/healthinfo/systems/en/)
Notification or reporting by a physician or other health care provider of the occurrence of specified contagious diseases such as tuberculosis and HIV infections to designated public health agencies. The United States system of reporting notifiable diseases evolved from the Quarantine Act of 1878, which authorized the US Public Health Service to collect morbidity data on cholera, smallpox, and yellow fever; each state in the US has its own list of notifiable diseases and depends largely on reporting by the individual health care provider. (From Segen, Dictionary of Modern Medicine, 1992)
The interaction of persons or groups of persons representing various nations in the pursuit of a common goal or interest.
Inflammation of the EPIDIDYMIS. Its clinical features include enlarged epididymis, a swollen SCROTUM; PAIN; PYURIA; and FEVER. It is usually related to infections in the URINARY TRACT, which likely spread to the EPIDIDYMIS through either the VAS DEFERENS or the lymphatics of the SPERMATIC CORD.
Inflammation of a TESTIS. It has many features of EPIDIDYMITIS, such as swollen SCROTUM; PAIN; PYURIA; and FEVER. It is usually related to infections in the URINARY TRACT, which likely spread to the EPIDIDYMIS and then the TESTIS through either the VAS DEFERENS or the lymphatics of the SPERMATIC CORD.
The thick, yellowish-white, viscid fluid secretion of male reproductive organs discharged upon ejaculation. In addition to reproductive organ secretions, it contains SPERMATOZOA and their nutrient plasma.
Infection caused by bacteria of the genus BRUCELLA mainly involving the MONONUCLEAR PHAGOCYTE SYSTEM. This condition is characterized by fever, weakness, malaise, and weight loss.
A cutaneous pouch of skin containing the testicles and spermatic cords.
Unstable isotopes of nitrogen that decay or disintegrate emitting radiation. N atoms with atomic weights 12, 13, 16, 17, and 18 are radioactive nitrogen isotopes.
A subfield of psychiatry that emphasizes the somatic substructure on which mental operations and emotions are based, and the functional or organic disturbances of the central nervous system that give rise to, contribute to, or are associated with mental and emotional disorders. (From Campbell's Psychiatric Dictionary, 8th ed.)
Acridine antineoplastic agent used in mammary and ovarian tumors. It inhibits RNA synthesis.
Diseases of domestic and mountain sheep of the genus Ovis.

Epitope mapping of the Brucella melitensis BP26 immunogenic protein: usefulness for diagnosis of sheep brucellosis. (1/18)

Sequencing of bp26, the gene encoding the Brucella sp. immunogenic BP26 periplasmic protein, was performed in the reference strains of Brucella abortus, B. suis, and B. ovis. The three bp26 sequences were almost identical to that published for B. melitensis 16M bp26, and only minor nucleotide substitutions, without modifying the amino acid sequence, were observed between species. The bp26 genes of the seven B. abortus biovar reference strains and B. abortus S19 and RB51 vaccine strains were also sequenced. Again, only minor differences were found. Surprisingly, the bp26 nucleotide sequence for B. abortus S19 was almost identical to that found for B. melitensis 16M and differed from the sequence described previously by others (O. L. Rossetti, A. I. Arese, M. L. Boschiroli, and S. L. Cravero, J. Clin. Microbiol. 34:165-169, 1996) for the same B. abortus strain. The epitope mapping of BP26, performed by using a panel of monoclonal antibodies and recombinant DNA techniques, allowed the identification of an immunodominant region of the protein interesting for the diagnosis of B. melitensis and B. ovis infection in sheep. A recombinant fusion protein containing this region of BP26 reacted indeed, in Western blotting, as the entire recombinant BP26 against sera from B. melitensis- or B. ovis-infected sheep while it avoided false-positive reactions observed with sera from Brucella-free sheep when using the entire recombinant BP26. Thus, use of this recombinant fusion protein instead the entire recombinant BP26 could improve the specific serological diagnosis of B. melitensis or B. ovis infection in sheep.  (+info)

Lack of evidence of Brucella ovis infection in rams in Quebec. (2/18)

A study was conducted to estimate the seroprevalence of Brucella ovis infection in rams in the Estrie and Bas-Saint-Laurent regions (Quebec). Rams sera (n = 258) were serologically evaluated from 224 rams in 30 commercial flocks and from 34 rams at 2 slaughterhouses by using an enzyme linked immunosorbent assay. Epididymides and testes were examined by palpation on farms and microscopically for culled rams. No ram was seropositive to Brucella ovis or had lesions suggestive of brucellosis from the farm or slaughterhouse surveys.  (+info)

Subcellular fractions of Brucella ovis distinctively induce the production of interleukin-2, interleukin-4, and interferon-gamma in mice. (3/18)

The aim of this study was to evaluate the effect of 3 Brucella ovis subcellular protein fractions: Outer membrane (OMP), inner membrane (IMP), and cytoplasm (CP), on cellular immune response by in vitro production of interleukin (IL)-2, IL-4, and interferon (IFN)-gamma. Each fraction was inoculated 3 times into Balb/c mice, primary cultures of mice spleen cells were done, and these were then stimulated with the fractions. Culture supernatants were collected at 24, 48, 72, 96, and 120 h postinoculation. Cytokine concentration was measured by Duoset-enzyme-linked immunosorbent assay (ELISA). The OMP fraction induced highest cellular immune response of 1000 pg/mL of IL-2 at 24 h, which decreased to < 100 pg/mL by 96 h. The IL-2 response for the IMP fraction was low at 24 h, but exceeded that of the OMP fraction at 72, 96, and 120 h. The CP showed a poor IL response. Regarding the IFN-gamma production, OMP and IMP induced a high response at 120 h. These results open the possibility for the use of B. ovis outer and inner membrane proteins as a subcellular vaccine.  (+info)

A DNA vaccine coding for the Brucella outer membrane protein 31 confers protection against B. melitensis and B. ovis infection by eliciting a specific cytotoxic response. (4/18)

The development of an effective subunit vaccine against brucellosis is a research area of intense interest. The outer membrane proteins (Omps) of Brucella spp. have been extensively characterized as potential immunogenic and protective antigens. This study was conducted to evaluate the immunogenicity and protective efficacy of the B. melitensis Omp31 gene cloned in the pCI plasmid (pCIOmp31). Immunization of BALB/c mice with pCIOmp31 conferred protection against B. ovis and B. melitensis infection. Mice vaccinated with pCIOmp31 developed a very weak humoral response, and in vitro stimulation of their splenocytes with recombinant Omp31 did not induced the secretion of gamma interferon. Splenocytes from Omp31-vaccinated animals induced a specific cytotoxic-T-lymphocyte activity, which leads to the in vitro lysis of Brucella-infected macrophages. pCIOmp31 immunization elicited mainly CD8(+) T cells, which mediate cytotoxicity via perforins, but also CD4(+) T cells, which mediate lysis via the Fas-FasL pathway. In vivo depletion of T-cell subsets showed that the pCIOmp31-induced protection against Brucella infection is mediated predominantly by CD8(+) T cells, although CD4(+)T cells also contribute. Our results demonstrate that the Omp31 DNA vaccine induces cytotoxic responses that have the potential to contribute to protection against Brucella infection. The protective response could be related to the induction of CD8(+) T cells that eliminate Brucella-infected cells via the perforin pathway.  (+info)

Vaccination with the recombinant Brucella outer membrane protein 31 or a derived 27-amino-acid synthetic peptide elicits a CD4+ T helper 1 response that protects against Brucella melitensis infection. (5/18)

The immunogenicity and protective efficacy of the recombinant 31-kDa outer membrane protein from Brucella melitensis (rOmp31), administered with incomplete Freund's adjuvant, were evaluated in mice. Immunization of BALB/c mice with rOmp31 conferred protection against B. ovis and B. melitensis infection. rOmp31 induced a vigorous immunoglobulin G (IgG) response, with higher IgG1 than IgG2 titers. In addition, spleen cells from rOmp31-immunized mice produced interleukin 2 (IL-2) and gamma interferon, but not IL-10 or IL-4, after in vitro stimulation with rOmp31, suggesting the induction of a T helper 1 (Th1) response. Splenocytes from rOmp31-vaccinated animals also induced a specific cytotoxic-T-lymphocyte activity, which led to the in vitro lysis of Brucella-infected macrophages. In vitro T-cell subset depletion indicated that rOmp31 immunization elicited specific CD4+ T cells that secrete IL-2 and gamma interferon, while CD8+ T cells induced cytotoxic-T-lymphocyte activity. In vivo depletion of T-cell subsets showed that the rOmp31-elicited protection against B. melitensis infection is mediated by CD4+ T cells while the contribution of CD8+ T cells may be limited. We then evaluated the immunogenicity and protective efficacy of a known exposed region from Omp31 on the Brucella membrane, a peptide that contains amino acids 48 to 74 of Omp31. Immunization with the synthetic peptide in adjuvant did not elicit a specific humoral response but elicited a Th1 response mediated by CD4+ T cells. The peptide in adjuvant induced levels of protection similar to those induced by rOmp31 against B. melitensis but less protection than was induced by rOmp31 against B. ovis. Our results indicate that rOmp31 could be a useful candidate for the development of subunit vaccines against B. melitensis and B. ovis.  (+info)

CGHScan: finding variable regions using high-density microarray comparative genomic hybridization data. (6/18)

BACKGROUND: Comparative genomic hybridization can rapidly identify chromosomal regions that vary between organisms and tissues. This technique has been applied to detecting differences between normal and cancerous tissues in eukaryotes as well as genomic variability in microbial strains and species. The density of oligonucleotide probes available on current microarray platforms is particularly well-suited for comparisons of organisms with smaller genomes like bacteria and yeast where an entire genome can be assayed on a single microarray with high resolution. Available methods for analyzing these experiments typically confine analyses to data from pre-defined annotated genome features, such as entire genes. Many of these methods are ill suited for datasets with the number of measurements typical of high-density microarrays. RESULTS: We present an algorithm for analyzing microarray hybridization data to aid identification of regions that vary between an unsequenced genome and a sequenced reference genome. The program, CGHScan, uses an iterative random walk approach integrating multi-layered significance testing to detect these regions from comparative genomic hybridization data. The algorithm tolerates a high level of noise in measurements of individual probe intensities and is relatively insensitive to the choice of method for normalizing probe intensity values and identifying probes that differ between samples. When applied to comparative genomic hybridization data from a published experiment, CGHScan identified eight of nine known deletions in a Brucella ovis strain as compared to Brucella melitensis. The same result was obtained using two different normalization methods and two different scores to classify data for individual probes as representing conserved or variable genomic regions. The undetected region is a small (58 base pair) deletion that is below the resolution of CGHScan given the array design employed in the study. CONCLUSION: CGHScan is an effective tool for analyzing comparative genomic hybridization data from high-density microarrays. The algorithm is capable of accurately identifying known variable regions and is tolerant of high noise and varying methods of data preprocessing. Statistical analysis is used to define each variable region providing a robust and reliable method for rapid identification of genomic differences independent of annotated gene boundaries.  (+info)

Improved immunogenicity of a vaccination regimen combining a DNA vaccine encoding Brucella melitensis outer membrane protein 31 (Omp31) and recombinant Omp31 boosting. (7/18)

In the present study, we report an attempt to improve the immunogenicity of the Omp31 antigen by a DNA prime-protein boost immunization regimen. We immunized BALB/c mice with an Omp31 DNA vaccine (pCIOmp31) followed by boosting with recombinant Omp31 (rOmp31) in incomplete Freund's adjuvant and characterized the resulting immune responses and the protective efficacy against Brucella ovis and B. melitensis infection. Immunoglobulin G1 (IgG1) and IgG2a titers were higher in sera from pCIOmp31/rOmp31-immunized mice than in sera from mice immunized with pCIOmp31 or rOmp31 alone. Splenocytes from pCIOmp31/rOmp31-immunized mice produced significantly higher levels of gamma interferon than did those from mice given rOmp31 alone. In contrast, interleukin 2 (IL-2) production levels were comparable between the two groups of immunized mice. Cells from all immunized mice produced undetectable levels of IL-4. Notably, rOmp31 stimulated IL-10 production in the pCIOmp31/rOmp31-immunized group but not in the pCIOmp31- or rOmp31-immunized group. Although the prime-boost regimen induced specific cytotoxic responses, these responses could not reach the levels achieved by the pCIOmp31 immunization. In conclusion, pCIOmp31 priming followed by rOmp31 boosting led to moderately improved protection against a challenge with B. ovis or B. melitensis.  (+info)

Role of the Omp25/Omp31 family in outer membrane properties and virulence of Brucella ovis. (8/18)

The genes coding for the five outer membrane proteins (OMPs) of the Omp25/Omp31 family expected to be located in the outer membrane (OM) of rough virulent Brucella ovis PA were inactivated to evaluate their role in virulence and OM properties. The OM properties of the mutant strains and of the mutants complemented with the corresponding wild-type genes were analyzed, in comparison with the parental strain and rough B. abortus RB51, in several tests: (i) binding of anti-Omp25 and anti-Omp31 monoclonal antibodies, (ii) autoagglutination of bacterial suspensions, and (iii) assessment of susceptibility to polymyxin B, sodium deoxycholate, hydrogen peroxide, and nonimmune ram serum. A tight balance of the members of the Omp25/Omp31 family was seen to be essential for the stability of the B. ovis OM, and important differences between the OMs of B. ovis PA and B. abortus RB51 rough strains were observed. Regarding virulence, the absence of Omp25d and Omp22 from the OM of B. ovis PA led to a drastic reduction in spleen colonization in mice. While the greater susceptibility of the Deltaomp22 mutant to nonimmune serum and its difficulty in surviving in the stationary phase might be on the basis of its dramatic attenuation, no defects in the OM able to explain the attenuation of the Deltaomp25d mutant were found, especially considering that the fully virulent Deltaomp25c mutant displayed more important OM defects. Accordingly, Omp25d, and perhaps Omp22, could be directly involved in the penetration and/or survival of B. ovis inside host cells. This aspect, together with the role of Omp25d and Omp22 in the virulence both of B. ovis in rams and of other Brucella species, should be thoroughly evaluated in future studies.  (+info)

Three flocks (all Romney Marsh) out of 13 were found to be infected, suggesting the disease had been introduced through sheep importations from New Zealand. A study of clinical lesions in the genital tract and of seminal quality made in these and in five of the uninfected flocks showed that Br. ovis infection is associated with a high incidence of epididymitis and orchitis, and a general deterioration in seminal quality with secondary morphological abnormalities of the spermatozoa being a prominent feature ...
Although there has been much written about the phenotype of rough mutants, those reports have relied upon the characterization of fortuitous rough isolates. Since nothing was known about the genetic defects in those organisms, any biological properties attributed to the lack of O antigen influenced the interpretation of subsequent experimental observations. For example, Brucella ovis andB. canis are naturally occurring rough species which are pathogenic in their preferred hosts and resistant to complement-mediated lysis (10, 46). As a result, most researchers have maintained that these organisms must express a shortened (somehow undetectable) O antigen (43). However, recent work in our laboratory with the broadly reactive anti-lipid A antibody (MAb 177) described in this paper indicates the presence of LPS in B. ovis and B. canis which is indistinguishable from that observed in the lps mutants (CA353, CA533, and CA613) of B. abortus (data not shown). The differences in phenotype (virulent versus ...
Moraxella ovis strain ATCC 33078 MovC (movC), RTX A toxin (movA), MovB (movB), MovD (movD), and putative secretion accessory protein (tolC) genes, complete ...
Biotin (vitamin H or vitamin B7) is the essential cofactor of biotin-dependent carboxylases, such as pyruvate carboxylase and acetyl-CoA carboxylase. Mammals cannot synthesize biotin, while in bacteria, fungi, and plants it is synthesized from pimelate thioester through different pathways. In E. coli and many organisms, pimelate thioester is derived from malonyl-ACP. The pathway starts with the methylation to malonyl-ACP methyl ester, followed by the fatty acid chain elongation cycle to form pimeloyl-ACP methyl ester, which is then demethylated to form pimeloyl-ACP [MD:M00572]. Pimeloyl-ACP is converted to biotin through the final four steps in the biotin bicyclic ring assembly, which are conserved among biotin-producing organisms [MD:M00123]. In B. subtilis, biotin is derived from pimeloyl-ACP formed by oxidative cleavage of long-chain acyl-ACPs [MD:M00573]. Some bacteria synthesize biotin from pimeloyl-CoA derived from pimelate [MD:M00577]. Biotin is covalently attached to biotin-dependent ...
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If youre a athletics fan, you might have heard of growth hormone - more commonly known as HGH - as well as associate the idea with shady and steroid use. Nonetheless did you know that HUMAN GROWTH HORMONE is a all-natural testosterone booster-style thats produced on its own and offers many crucial benefits? It can true. Hgh is naturally created in the pituitary gland and also plays a huge role in mobile regeneration, progress and keeping healthy human being tissue, like that of the brain and several vital bodily organs. Once produced, HGH is still active in the system for a few short minutes, allowing adequate time for the particular liver for converting it in to growth elements, the most crucial getting insulin-like growing factor (IGF-1), which has growth-promoting properties in each cell in the body. Information taken from www.steroidsonline.org/hgh-for-sale/ ...
Preserve hair and skins natural balance with the nourishing and revitalizing Ovis Shampoo Bar. FEATURES Compact and long-lasting Paraben, silicon, mineral, and palm oil-free Fragrance-free Sensitive Bar Enriched with pure vegetable oils Sheep milk helps cleanse hair providing natural nutrients Foam like soap and rins
Ann Inst Pasteur Microbiol 1987,138(2):235-238.PubMedCrossRef 11. Grimont F, Verger JM, Cornelis Kinase Inhibitor Library cell line P, Limet J, Lefevre M, Grayon M, Regnault B, Van Broeck J, Grimont PA: Molecular typing of Brucella with cloned DNA probes. Res Microbiol 1992,143(1):55-65.PubMedCrossRef 12. Cerri D, Ebani VV, Pedrini A, Nuvoloni R, Renzoni G, Andreani E, Farina R: Epididymitis by Brucella ovis : experimental infection in virgin ram lambs. New Microbiol 1999,22(3):227-231.Z-IETD-FMK price PubMed 13. Davis CE, Troy SB: Brucellosis. N Engl J Med 2005,353(10):1071-1072. author reply 1071-1072PubMedCrossRef 14. Fenkci V, Cevrioglu S, Yilmazer M: Ovarian abscess due to Brucella melitensis . Scand J Infect Dis 2003,35(10):762-763.PubMedCrossRef. 15. Pappas G, Akritidis N, Bosilkovski M, Tsianos E: Brucellosis. N Engl J Med 2005,352(22):2325-2336.PubMedCrossRef 16. Troy SB, Rickman CP-690550 LS, Davis CE: Brucellosis in San Diego: epidemiology and species-related differences in acute ...
Meanwhile, researchers in China have described the first outbreak of brucellosis in Zhangping City, Fujian Province.. Six confirmed cases were found. One patient experienced onset of symptoms in April while the others fell sick from late May to mid-June 2019, according to a study published in China CDC weekly.. The investigation suggested the transmission chain included a private butcher, an infected goat, a dairy farmer, close contact spread, unsterilized goat milk, and consumers drinking raw goat milk.. In July, Zhangping City officials reported an outbreak of brucellosis in a family. A mother and daughter had drunk goat milk produced and bottled from a local farmer. The city only reported one case of human brucellosis in both 2011 and 2017.. From the six confirmed cases, one of the blood specimens was cultured as Brucella ovis. All patients had symptoms of fatigue, five also had fever, two had abnormally excessive sweating and muscle aches, and one had vomiting, chills and headache.. Five ...
Antisera against Actinobacillus seminis, Brucella ovis and Corynebacterium pseudotuberculosis were prepared in adult female goats. Specific immunofluorescence was observed in cultural smears of A seminis, B ovis and C pseudotuberculosis by the direct technique and in smears of A seminis also by the indirect technique. Individual organisms could be recognised. Specific fluorescence of A seminis was readily detected in semen. The results indicate that immunofluorescence may offer an effective method for rapidly and accurately diagnosing bacterial epididymitis in sheep, especially before epididymal lesions are palpable.. ...
Looking for online definition of Neisseria ovis in the Medical Dictionary? Neisseria ovis explanation free. What is Neisseria ovis? Meaning of Neisseria ovis medical term. What does Neisseria ovis mean?
Efecto del uso de medio secuencial humano en la producción de blastocistos de hembra ovis canadensis mexicana por clonación manual
The primers for the genes of the identified proteins were designed and amplificated by PCR in four Brucella strains. The optimized PCR conditions and the length of PCR products were determined. The results of the PCR amplification in four different Brucella strains were shown. +, Corresponding sequence have been able to amplify; -, Corresponding sequence not been able to amplify ...
The articles have been scanned in colour with a HP Scanjet 5590; 300dpi. Adobe Acrobat XI Pro was used to OCR the text and also for the merging and conversion to the final presentation PDF-format ...
When and how often laboratory tests are done may depend on many factors. The timing of laboratory tests may rely on the results or completion of other tests, procedures, or treatments. Lab tests may be performed immediately in an emergency, or tests may be delayed as a condition is treated or monitored. A test may be suggested or become necessary when certain signs or symptoms appear. Due to changes in the way your body naturally functions through the course of a day, lab tests may need to be performed at a certain time of day. If you have prepared for a test by changing your food or fluid intake, lab tests may be timed in accordance with those changes. Timing of tests may be based on increased and decreased levels of medications, drugs or other substances in the body. The age or gender of the person being tested may affect when and how often a lab test is required. Chronic or progressive conditions may need ongoing monitoring through the use of lab tests. Conditions that worsen and improve may ...
This entry consists of various amine oxidases, including maize polyamine oxidase (PAO) [ (PUBMED:9598979) ], L-amino acid oxidases (LAO) and various flavin containing monoamine oxidases (MAO). The aligned region includes the flavin binding site of these enzymes. In vertebrates MAO plays an important role in regulating the intracellular levels of amines via their oxidation; these include various neurotransmitters, neurotoxins and trace amines [ (PUBMED:9162023) ]. In lower eukaryotes such as aspergillus and in bacteria the main role of amine oxidases is to provide a source of ammonium [ (PUBMED:7770050) ]. PAOs in plants, bacteria and protozoa oxidise spermidine and spermine to an aminobutyral, diaminopropane and hydrogen peroxide and are involved in the catabolism of polyamines [ (PUBMED:9598979) ]. Other members of this family include tryptophan 2-monooxygenase, putrescine oxidase, corticosteroid binding proteins and antibacterial glycoproteins. ...
Looking for online definition of Ovis aries in the Medical Dictionary? Ovis aries explanation free. What is Ovis aries? Meaning of Ovis aries medical term. What does Ovis aries mean?
Pathogens infecting mammalian cells have developed various strategies to suppress and evade their hosts defensive mechanisms. In this line, the intracellular bacteria that are able to survive and propagate within their host cells must have developed strategies to avert their hosts killing attitude. Studying the interface of host-pathogen confrontation can provide valuable information for defining therapeutic approaches. Brucellosis, caused by the Brucella strains, is a zoonotic bacterial disease that affects thousands of humansand animals around the world inflicting discomfort and huge economic losses. Similar to many other intracellular dwelling bacteria, infections caused by Brucella are difficult to treat, and hence any attempt at identifying new and common therapeutic targets would prove beneficial for the purpose of curing infections caused by the intracellular bacteria. In THP-1 macrophage infected with Brucella melitensis we studied the expression levels of four hosts genes, i.e. EMP2, ST8SIA4
Fingerprint Dive into the research topics of Replication of Brucella abortus and Brucella melitensis in fibroblasts does not require Atg5-dependent macroautophagy. Together they form a unique fingerprint. ...
Brucella spp. are the etiological agents of brucellosis, a zoonotic infectious disease that causes abortion in animals and chronic debilitating illness in humans. Natural Brucella infections occur primarily through an incompletely defined mechanism of adhesion to and penetration of mucosal epithelium. In this study, we characterized changes in genome-wide transcript abundance of the most and the least invasive growth phases of B. melitensis cultures to HeLa cells, as a preliminary approach for identifying candidate pathogen genes involved in invasion of epithelial cells. B. melitensis at the late logarithmic phase of growth are more invasive to HeLa cells than mid-logarithmic or stationary growth phases. Microarray analysis of B. melitensis gene expression identified 414 up- and 40 down-regulated genes in late-log growth phase (the most invasive culture) compared to the stationary growth phase (the least invasive culture). As expected, the majority of up-regulated genes in late-log phase cultures were
Brucella Species answers are found in the Johns Hopkins ABX Guide powered by Unbound Medicine. Available for iPhone, iPad, Android, and Web.
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LAA049Ov81, FITC-Linked Polyclonal Antibody to Interferon Gamma (IFNg), 干扰素γ(IFNg)多克隆抗体(异硫氰酸荧光素标记), IFN-G; IFG; IFI; INFr; IFN, Immune Interferon | 仅供体外研究使用,不用于临床诊断!请索取进口关税税单及报关单!
Exerts, in vitro, a potent antimicrobial activity. Probably due to an impairment of the function of the respiratory chain and of energy-dependent activities in the inner membrane of susceptible microorganisms (By similarity).
CEA441Ov, ELISA Kit for Luteinizing Hormone (LH), 黄体激素(LH)检测试剂盒(酶联免疫吸附试验法), ICSH; Lutropin; Interstitial Cell Stimulating Hormone | 仅供体外研究使用,不用于临床诊断!请索取进口关税税单及报关单!
Opens the Highlight Feature Bar and highlights feature annotations from the FEATURES table of the record. The Highlight Feature Bar can be used to navigate to and highlight other features and provides links to display the highlighted region separately. Links in the FEATURES table will also highlight the corresponding region of the sequence. More... ...
Opens the Highlight Feature Bar and highlights feature annotations from the FEATURES table of the record. The Highlight Feature Bar can be used to navigate to and highlight other features and provides links to display the highlighted region separately. Links in the FEATURES table will also highlight the corresponding region of the sequence. More... ...
Zylexis EQ (Parapox Ovis Virus Immunomodulator) is for use in healthy horses 4 months of age or older as an aid in reducing upper respiratory disease caused by equine herpesvirus types 1 and 4.
Brucella BvrR protein: BvrR/BvrS represent the two-component system involved in the regulation of Brucella virulence; amino acid sequence in first source
Although not all of the differentiating genes identified were transcribed under steady state conditions, a group of genes sufficient to discriminate unambiguously between B. suis, B. melitensis, and B. abortus was identified. We present an overview of these genomic differences and the use of these f …
FUN_05: Use the SSGCID solved structure of Brucella melitensis methionine-tRNA-synthetase (MetRS) to find inhibitors for potential drug development for brucellosis.
This is the microbiology questions and answers section on Brucella with explanation for various interview, competitive examination and entrance test. Solved examples with detailed answer description, explanation are given and it would be easy to understand.
Brucella melitensis bacteria cause persistent, intracellular infections in small ruminants as well as in humans, leading to significant morbidity and economic loss worldwide. The majority of experiments on the transcriptional responses of Brucella to conditions inside the host have been performed following invasion of cultured mammalian cells, and do not address gene expression patterns during long-term infection. Here, we examine the application of the previously developed coincidence cloning methodology to recover and characterize B. melitensis RNA from the supramammary lymph node of experimentally-infected goats. Using coincidence cloning, we successfully recovered Brucella RNA from supramammary lymph nodes of B. melitensis-infected goats at both short-term (4 weeks) and long-term (38 weeks) infection time points. Amplified nucleic acid levels were sufficient for analysis of Brucella gene expression patterns by RNA-sequencing, providing evidence of metabolic activity in both the short-term and the
This article refers to the sheep genus. For the species commonly referred to as sheep, see sheep (Ovis aries). Ovis is a genus of mammals, part of the goat-antelope subfamily of the ruminant family Bovidae. Its five or more highly sociable species are known as sheep. The domestic sheep is one member of the genus, and is thought to be descended from the wild mouflon of central and southwest Asia. Female sheep are called ewes, males are called rams (sometimes also called bucks or tups), and young sheep are called lambs. The adjective applying to sheep is ovine, and the collective term for sheep is flock or mob. The term herd is also occasionally used in this sense. Many specialist terms relating to domestic sheep are used. Sheep are usually stockier than most other bovines, and their horns are usually divergent and curled into a spiral. Sheep have scent glands on their faces and feet. Communication through the scent glands is not well understood, but is thought to be important for sexual ...
Curated}} {{Biorealm Genus}} [[Image:brucella_close_up.JPG,thumb,400px,right,Electron micrograph of B. abortus located inside cisternae of Vero cell. Within the perinuclear envelope, the cisternae containing B. abortus are discontinuously lined by ribosomes (arrows). From [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2114362 Detilleux et al.]]] ==Classification== ===Higher order taxa:=== Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Brucellaceae ===Species:=== Brucella abortus, Brucella canis, Brucella cetaceae, Brucella maris , Brucella melitensis, Brucella pinnipediae, Brucella sp. {, , height=10 bgcolor=#FFDF95 align=center , NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=234 Taxonomy] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=genomeprj&cmd=Search&dopt=DocSum&term=txid234%5BOrganism:exp%5D Genomes] ,} ==Description and Significance== Brucella sp. causes brucellosis, ...
We present a rare case of brucella endocarditis, forming a vegetation on the mitral valve. The definitive diagnosis has been made with clinical suspicion, positive serology, the demonstration of the vegetation with the echocardiography and with the production from the multiple blood culture of brucella melitensis and from the excised valve. Our patient has been successfully treated with specific antibiotherapy and the surgery of replacement of mitral valve. Our aim in presenting the case is to remind the infective endocarditis which is due to this factor in the regions like our country which is endemic for ...
The Cyprian wild sheep - the national animal of Cyprus - was probably introduced by man from Asia minor to Cyprus about 8000 to 10000 years ago. With an average shoulder height of 66 cm, the Cyprus mouflon is the smallest of all wild sheep. There is a clear sexual dimorphism: males (body-weight 30-40 kg) are larger than the females (body-weight 20-30 kg) and have much larger horns, which may reach a length of 71 cm. ...
BIOSYNTHESE + ANABOLISMUS (MIKROBIOLOGIE); PROTEINBIOSYNTHESE + PROTEINANABOLISMUS + PEPTIDBIOSYNTHESE + PEPTIDANABOLISMUS (METABOLISMUS); PANSENFLORA (TIERPHYSIOLOGIE, TIERMORPHOLOGIE); SCHAFE + SCHAFZUCHT + SCHAFHALTUNG (TIERHALTUNG); OVIS (ZOOLOGIE); HARNCHEMIE (TIERPHYSIOLOGIE); PURINMETABOLISMUS; BIOSYNTHESIS + ANABOLISM (MICROBIOLOGY); PROTEIN BIOSYNTHESIS + PROTEIN ANABOLISM + PEPTIDE BIOSYNTHESIS + PEPTIDE ANABOLISM (METABOLISM); RUMEN FLORA (ANIMAL PHYSIOLOGY, ANIMAL MORPHOLOGY); SHEEP + SHEEP BREEDING + SHEEP HUSBANDRY (ANIMAL HUSBANDRY); OVIS (ZOOLOGY); URINE CHEMISTRY (ANIMAL PHYSIOLOGY); PURINE ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Journal of Wildlife Diseases publishes work on infectious, parasitic, toxic, nutritional, physiologic, and neoplastic diseases impacting wild animals.
PCR-RFLP analysis of Brucella LPS genes manAO-Ag, manBO-Ag, wbkD, wbkF, wboA and wa**. Panel A. Lanes: 1, molecular size markers; 2, manAO-Ag from B. melitensis
Left mandible of sheep (Ovis aries)Photo author: Albert Fischer - Date: 27/04/2014.For more information, refer to the presentation of the corpus of the author. - Ovis-Jaw-Left.jpg
The patterns of susceptibility to hydrophobic and hydrophilic drugs and the uptake of the fluorescent probe N-phenyl-naphthylamine in Brucella spp., Haemophilus influenzae, Escherichia coli, and deep rough Salmonella minnesota mutants were compared. The results show that the outer membranes of smooth and naturally rough Brucella spp. do not represent barriers to hydrophobic permeants and that this absence of a barrier relates at least in part to the properties of Brucella lipopolysaccharide. ...
Ophthalmomyiasis externa is mainly caused by the sheep botfly Oestrus ovis; therefore, it is usually seen in rural areas. We report here a case of ophthalmomyiasis externa in a young man from Istanbul. The patient had no known history of exposure to farm animals or rural areas. ...
We report a case of Brucella abortus-induced brucellosis that displayed high hepatic transaminases similar to that in acute hepatitis, fourth month relapsed following doxycycline-streptomycin treatment, and developed doxycycline-related hepatotoxicity in second treatment. Brucella hepatitis may occur with various Brucella spp. and may relapse even following ...
... in New Zealand is limited to sheep (B. ovis). The country is free of all other species of Brucella. Dairy herds in ... Brucella species survive well in aerosols and resist drying. Brucella and all other remaining biological weapons in the U.S. ... ovis (sheep), and B. suis (caribou and pigs). Brucella species have also been isolated from several marine mammal species ( ... Brucella suis, also referred to as swine brucellosis Wyatt HV (2014). "How did Sir David Bruce forget Zammit and his goats ?" ( ...
The California Red Sheep Registry requires their members to test rams for ovine brucellosis (Brucella ovis), a bacterial ...
viciae 3841 strain, and the Agrobacterium species A. vitis,A. tumefaciens, A. radiobacter and A. H13, Brucella species (B. ovis ... as well as in a broad spectrum of Brucella species (B. ovis, B. canis, B. abortus and B. microtis, and several viobars of B. ... Partial synteny of the αr45 genomic regions was observed in the Mesorhizobium and Brucella species where instead of aLysR ... This analysis also revealed an extended conserved sequence stretch among the promoters of the Brucella and Bartonella αr45 sRNA ...
... as well as in a broad spectrum of Brucella species (B. ovis, B. canis, B. abortus and B. microtis, and several viobars of B. ... but lower bit-scores and are encoded by Brucella species (B. ovis, B. canis, B. abortus, B. microtis, and several biobars of B ... The second group is formed by the first copy in the Chromosome I in the Brucellas genomes that share the gene upstream coding ... and the second copy in chromosome I in the Brucella group (Bor14CI2, Bcr14CI2, Bmir14CI2, Bs1330r14CI2, Bm16Mr14CI1, ...
... as well as in a broad spectrum of Brucella species (B. ovis, B. canis, B. abortus and B. microtis, and several viobars of B. ... but lower bit-scores and are encoded by Brucella species (B. ovis, B. canis, B. abortus, B. microtis, and several biobars of B ... A special case is the Brucella group, where primary automatic annotation over their genomes identified ORFs smaller than 30 aa ...
Brucella ovis ATCC 25840 chromosome I (NC_009505), Bmir7CI = Brucella microti CCM 4915 chromosome 1 (NC_013119), Oar7CI = ... 16M chromosome I (NC_003317), BaS19r7CI = Brucella abortus S19 chromosome 1 (NC_010742), Bm23457r7CI = Brucella melitensis ATCC ... as well as in a broad spectrum of Brucella species (B. ovis, B. canis, B. abortus and B. microtis, and several biobars of B. ... but lower bit-scores and are encoded by Brucella species (B. ovis, B. canis, B. abortus, B. microtis, and several biobars of B ...
Brucella canis MeSH B03.440.400.425.215.500.500 - Brucella melitensis MeSH B03.440.400.425.215.500.625 - Brucella ovis MeSH ... Brucella canis MeSH B03.660.050.070.100.500 - Brucella melitensis MeSH B03.660.050.070.100.625 - Brucella ovis MeSH B03.660. ... Brucella MeSH B03.440.400.425.215.500.100 - Brucella abortus MeSH B03.440.400.425.215.500.150 - ... Brucella MeSH B03.660.050.070.100.100 - Brucella abortus MeSH B03.660.050.070.100.150 - ...
3,279 ORFs Brucella microti CCM 4915, 3,346 ORFs Brucella ovis ATCC 25840, 3,193 ORFs Brucella pinnipedialis B2/94, 3,505 ORFs ... Brucella abortus A13334, 3,401 ORFs Brucella canis ATCC 23365, 3,408 ORFs Brucella melitensis 16M, ... Brucella species have been found primarily in mammals: Pathogenic Brucella species can cause abortion in female animals by ... Conversely, depriving Brucella of the blue wavelengths dropped its reproductive rate by 90%. Parte, A.C. "Brucella". Muleme.M ...
... and other Brucella species (B. melitensis, B. abortis, B. ovis, B. canis) are recognized as potential ... The Brucella abortus (rough LPS Brucella) vaccine, developed for bovine brucellosis and licensed by the USDA Animal Plant ... of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella ... Brucella suis is a bacterium that causes swine brucellosis, a zoonosis that affects pigs. The disease typically causes chronic ...
... one being Brucella abortus. Some of the other species are known as B. melintisis, B. canis, B. suis, B. ovis, B. neotomae, B. ... "Humans and Brucella Species". "Brucellosis: Brucella Abortus" (PDF). Kaden, R.; Ferrari, S.; Jinnerot, T.; Lindberg, M.; Wahab ... Brucella abortus is a Gram-negative alpha-proteobacterium in the family Brucellaceae and is one of the causative agents of ... Brucella abortus enters phagocytes that invade human and animal innate defenses which in turn, cause chronic disease in the ...
Ocampo-Sosa, Alain A; García-Lobo, Juan M (2008). "Demonstration of IS711 transposition in Brucella ovis and Brucella ... and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts". International ... LPSN "Brucella pinnipedialis" at the Encyclopedia of Life Type strain of Brucella pinnipedialis at BacDive - the Bacterial ... "The genome sequence of Brucella pinnipedialis B2/94 sheds light on the evolutionary history of the genus Brucella". BMC ...
The bacterium causes ovine brucellosis, along with Brucella ovis. It affects primarily sheep and goats, but cases have also ... Brucella melitensis genomes and related information at PATRIC, a Bioinformatics Resource Center funded by NIAID Brucella ... The genus of Micrococcus was later renamed Brucella, in honor of David Bruce. The bacterium was detected in a 3200-year-old ... "brucellosis (Brucella melitensis)". www.cabi.org. Retrieved 22 March 2018. "Brucellosis in Humans: Symptoms, Treatment, Cause ...
... is a Gram-negative coccobacillus from the Brucellaceae family. Along with Brucella melitensis, it is responsible ... Brucella ovis, reviewed and published by Wikivet at http://en.wikivet.net/Brucella_ovis, accessed 24/08/2011. v t e v t e. ... B. ovis can be transmitted by the stable fly. Infection causes severe inflammation of the epididymis, particularly the tail. ...
Elands produce antibodies for Brucella bacteria, but none for Mycobacterium paratuberculosis or various types of pneumonia like ... Ovis. *Argali (O. ammon). *Domestic sheep (O. aries). *Bighorn sheep (O. canadensis) ...
Brucellosis is caused by different biotypes of Brucella abortus and B. melitensis.[52] Other internal parasites include ... Ovis. *Argali (O. ammon). *Domestic sheep (O. aries). *Bighorn sheep (O. canadensis) ...
Brucella ovis is a Gram-negative coccobacillus from the Brucellaceae family. Along with Brucella melitensis, it is responsible ... Brucella ovis, reviewed and published by Wikivet at http://en.wikivet.net/Brucella_ovis, accessed 24/08/2011. v t e v t e. ... B. ovis can be transmitted by the stable fly. Infection causes severe inflammation of the epididymis, particularly the tail. ...
Biotin metabolism - Brucella ovis [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show description , User data ...
1985)‎. Proposed international standard for anti-brucella ovis serum / The Central Veterinary Laboratory, Weybridge, Surrey, ... Proposed international standard for anti-brucella ovis serum / The Central Veterinary Laboratory, Weybridge, Surrey, United ... Metabolic and bacteriophage identification of Brucella strains described as Brucella melitensis from cattle*  Meyer, Margaret ... The Second International Standard for Anti-Brucella abortus Serum*  Davidson, Ian; Hebert, C. Nancy; Morgan, W. J. Brinley (‎ ...
Ovis canadensis canadensis). Antibodies to B. ovis, however, are occasionally detected in free-ranging BHS, and this has been a ... Disease caused by Brucella ovis has not been previously reported in bighorn sheep (BHS; ... Disease caused by Brucella ovis has not been previously reported in bighorn sheep (BHS; Ovis canadensis canadensis). Antibodies ... Clinical, culture, serology, and histopathology outcomes of bighorn sheep experimentally infected with Brucella ovis J Wildl ...
Brucella ovis ATCC 25840. Mutation(s): 2 Gene Names: galE-1, BOV_A0474. EC: 5.1.3.2. ... Crystal structure of UDP-glucose 4-epimerase from Brucella ovis in complex with NAD. *DOI: 10.2210/pdb4WOK/pdb ... Find proteins for A0A0H3ASR6 (Brucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512)) ... Crystal structure of UDP-glucose 4-epimerase from Brucella ovis in complex with NAD. SSGCID, Seattle Structural Genomics Center ...
brucellosis (Brucella ovis). International Common Names. English. Brucella ovis ram epididymitis, brucellosis in sheep, ... Brucella ovis is the least pathogenic of all the Brucella species that infect domestic animals. It exclusively infects sheep ... Seroprevalence of Brucella ovis, Brucella melitensis and Chlamydia psittaci in rams. Indian Journal of Animal Sciences, 66(11): ... pathology and histology of Brucella ovis infection. Briefly: as with other Brucella infections there is a period of generalized ...
... from Brucella ovis in complex with a partially ordered NAD ... Find proteins for A0A0H3AQ75 (Brucella ovis (strain ATCC 25840 ... from Brucella ovis in complex with a partially ordered NAD. Abendroth, J., Dranow, D.M., Lorimer, D.D., Edwards, T.E.. To be ... Crystal structure of an Oxidoreductase (short chain dehydrogenase/reductase family) from Brucella ovis in complex with a ... Brucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512). Mutation(s): 0 ...
Brucella abortus biovar 1 (strain 9-941). Brucella abortus bv. 5 str. B3196. Brucella abortus NCTC 8038. Brucella abortus str. ... Brucella canis (strain ATCC 23365 / NCTC 10854). Brucella melitensis biotype 2 (strain ATCC 23457). Brucella melitensis biotype ... Brucella ovis IntaBari-2002-82-58. 538. UniRef100_A5VSF8. Cluster: Bifunctional purine biosynthesis protein PurH. 2. ... sp,A5VSF8,PUR9_BRUO2 Bifunctional purine biosynthesis protein PurH OS=Brucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512) ...
Brucella ovis Gram negative coccobacillus Facultative intracellular pathogen Slideshow 4806253 by shania ... Brucella ovis. Overview. Organism History Epidemiology Transmission Disease in Animals Prevention and Control Actions to Take. ... Ovine Epididymitis: Brucella ovis. Overview. Organism History Epidemiology Transmission Disease in Animals Prevention and ... The Organism • Brucella ovis • Gram negativecoccobacillus • Facultativeintracellular pathogen • Persists in the environment ...
Antibody response to Brucella ovis outer membrane proteins in ovine brucellosis.. J I Riezu-Boj, I Moriyón, J M Blasco, C ... Antibody response to Brucella ovis outer membrane proteins in ovine brucellosis.. J I Riezu-Boj, I Moriyón, J M Blasco, C ... Antibody response to Brucella ovis outer membrane proteins in ovine brucellosis.. J I Riezu-Boj, I Moriyón, J M Blasco, C ... Antibody response to Brucella ovis outer membrane proteins in ovine brucellosis. Message Subject (Your Name) has forwarded a ...
Brucella ovis) (OPCA101054) , Recombinant Protein , Application: WB, ELISA , Species Reactivity: Brucella ovis , Alias: ...
Adaptação e avaliação da reação em cadeia da polimerase para detecção de Brucella ovis em sêmen, urina e órgãos de carneiros ... Multipelx PCR for the detection of Brucella ovis, Actinobacillus seminis and Histophilus somni in ram semen. Aust. Vet. J., v. ... WEBB, R.F.; QUINN, C.A.; COCKRAM, F.A. et al. Evaluation of procedures for the diagnosis of Brucella ovis infection in rams. ... Adaptation and evaluation of polymerase chain reaction for Brucella ovis detection in semen, urine and organs of rams ...
Clapp (1955) showed the complement fixation test (C.F.T.) for Brucella ovis in sheep to be well correlated to bacteriological ... the test for Brucella ovis has been automated. This paper outlines the method of automation and its correlation with the manual ...
Find out information about Brucella ovis. A genus of gram-negative, aerobic bacteria of uncertain affiliation; single, ... nonmotile coccobacilli or short rods, all of which are parasites and pathogens... Explanation of Brucella ovis ... Related to Brucella ovis: Brucella abortus, Brucella canis, Brucella melitensis, Brucella neotomae Brucella. [brü′sel·ə] ( ... Brucella ovis , Article about Brucella ovis by The Free Dictionary https://encyclopedia2.thefreedictionary.com/Brucella+ovis ...
Type III secretion homologs are present in Brucella melitensis, B-ovis, and B-suis biovars 1, 2, and 3 ... Type III secretion homologs are present in Brucella melitensis, B-ovis, and B-suis biovars 1, 2, and 3 CURRENT MICROBIOLOGY, 46 ...
Experimental Infection by Brucella ovis: Changes in NTPDase, 5-Nucleotidase and Acetylcholinesterase Associated Cerebral ...
Mice vaccinated with the B. ovis mutants developed anti-B. ovis antibodies in serum of the IgG1, IgG2a and IgG2b subclasses and ... Additionally, the B. ovis mutants showed appropriate persistence, limited splenomegaly and protective efficacy against B. ovis ... ovis infection, but its use for this purpose has serious drawbacks. In this work, two previously characterized B. ovis ... Attenuated Brucella melitensis Rev 1, used as vaccine against ovine and caprine brucellosis caused by B. melitensis, is also ...
O trabalho teve como objetivo estudar a patogenia da Brucella ovis, em carneiros infectados experimentalmente com a cepa REO ... The aim of this study was the identification of Brucella ovis REO 198 strain pathogenic effect in rams experimentally infected ... Lesões anatomopatológicas e detecção da Brucella ovis cepa REO 198 em ovinos inoculados experimentalmente pelas vias ... A total of 31 rams with 1-2 years old were challenged by intraconjuntival and intrapreputial inoculation using a B. ovis ...
Some Brucella species infect certain marine mammals. Most humans who get brucellosis get it from dairy animals or dairy ... B. abortus infects domestic livestock (cattle, bison, and elk); B. melitensis, goats and sheep; B. ovis, sheep; and B. suis, ... Brucellosis is a disease caused by Brucella bacteria of several species. Different species tend to infect different animals. ...
Three types of the bacteria that cause brucellosis - Brucella abortus, Brucella melitensis and Brucella suis - are designated ... Exposure to most species of Brucella, such as those associated with certain types of animals, could potentially lead to ... Human Neurobrucellosis with Intracerebral Granuloma Caused by a Marine Mammal Brucella spp. ... Brucella ovis and neotomae. *Not known to be pathogenic for humans. Brucella suis* ...
Dénes B, Glávits R. Bacteriologically confirmed cases of ovine epididymo-orchitis caused by Brucella ovis in Sub-Carpathia. ... Bacteriologically confirmed cases of ovine epididymo-orchitis caused by Brucella ovis in Sub-Carpathia. / Dénes, B.; Glávits, R ... Dénes, B., & Glávits, R. (1994). Bacteriologically confirmed cases of ovine epididymo-orchitis caused by Brucella ovis in Sub- ... Dénes, B. ; Glávits, R. / Bacteriologically confirmed cases of ovine epididymo-orchitis caused by Brucella ovis in Sub- ...
Brucella ovis infection. Caprine and ovine brucellosis (B. melitensis). Caprine arthritis/encephalitis. Contagious agalactia. ...
Ovine epididymitis (Brucella ovis). Present. Peste des petits ruminants. Free. Never occurred ...
Ovine epididymitis (Brucella ovis) *Peste des petits ruminants. *Salmonellosis (S. abortusovis) *Scrapie ...
Fingerprint Dive into the research topics of Genome degradation in Brucella ovis corresponds with narrowing of its host range ... Genome degradation in Brucella ovis corresponds with narrowing of its host range and tissue tropism. ...
... ... ovis infection is associated with a high incidence of epididymitis and orchitis, and a general deterioration in seminal quality ...
Ovine epididymitis (Brucella ovis). *Ovine pulmonary adenomatosis. *Paratuberculosis (Johnes disease). *Porcine reproductive ...
... ovis in phagocytes was analyzed. The absence of Omp25d or Omp22 in B. ovis abolished its invasive capacity in HeLa cells and r ... Brucella ovis / physiology*. Brucellosis / microbiology. Hela Cells / microbiology*. Humans. Macrophages / microbiology*. Mice ... ovis in phagocytes was analyzed. The absence of Omp25d or Omp22 in B. ovis abolished its invasive capacity in HeLa cells and ... These findings demonstrate that Omp25d and Omp22 are essential for the invasion and survival of B. ovis inside host cells, and ...
Full text: Available Index: LILACS (Americas) Main subject: Sheep / Immunodiffusion / Brucella ovis / Diagnosis Limits: Animals ... Full text: Available Index: LILACS (Americas) Main subject: Sheep / Immunodiffusion / Brucella ovis / Diagnosis Limits: Animals ... The aim of this investigation was to determine the seroprevalence of Brucella ovis in sheep flocks and individual sheep in the ... Animals , Brucella ovis/isolation & purification , Diagnosis , Immunodiffusion/veterinary , Sheep/microbiology , Epidemiologic ...
... ovis) is a contagious disease that impairs rams fertility due to epididymis, testicle and accessory sexual gland alterations. ... Mutants in the lipopolysaccharide of Brucella ovis are attenuated and protect against B ovis infection in mice. Vet Res. 2014; ... Contagious Epididymitis (CE) due to Brucella ovis (B. ovis) is a contagious disease that impairs rams fertility due to ... Contagious Epididymitis (CE) due to Brucella ovis (B. ovis) is a contagious disease of worldwide importance that impairs ...
  • Along with Brucella melitensis, it is responsible for causing ovine brucellosis, which is a notifiable disease. (wikipedia.org)
  • Antibody response to Brucella ovis outer membrane proteins in ovine brucellosis. (asm.org)
  • The manual C.F.T. places considerable demands on trained and experienced laboratory staff, and in the light of experience gained during the New Zealand Bovine Brucellosis Testing Scheme with automated serology, the test for Brucella ovis has been automated. (sciquest.org.nz)
  • Attenuated Brucella melitensis Rev 1, used as vaccine against ovine and caprine brucellosis caused by B. melitensis , is also considered the best vaccine available for the prophylaxis of B. ovis infection, but its use for this purpose has serious drawbacks. (biomedcentral.com)
  • Brucellosis caused by Brucella ovis mainly induces chronic epididymitis and orchitis in rams. (biomedcentral.com)
  • Brucella melitensis Rev 1 is an S live attenuated strain currently used for vaccination campaigns against ovine and caprine brucellosis, which is almost exclusively caused by B. melitensis and B. ovis . (biomedcentral.com)
  • Three types of the bacteria that cause brucellosis - Brucella abortus , Brucella melitensis and Brucella suis - are designated as select agents. (cdc.gov)
  • Brucellosis is a disease caused by Brucella bacteria of several species. (cdc.gov)
  • The bacterium causes ovine brucellosis, along with Brucella ovis. (wikipedia.org)
  • It is zoonotic, unlike B. ovis, causing Malta fever or localized brucellosis in humans. (wikipedia.org)
  • Ovine and Caprine Brucellosis: Brucella melitensis" (PDF). (wikipedia.org)
  • Brucella is the cause of brucellosis, which is a zoonosis transmitted by ingesting contaminated food (such as unpasteurized milk products), direct contact with an infected animal, or inhalation of aerosols. (wikipedia.org)
  • Uterine discharges were collected from the bitch and the cat under sterile conditions after positive Brucella serology using Rose Bengal Test and Complement Fixation Test and sent to the OIE reference laboratory of brucellosis at the Friedrich-Loeffler-Institut for Brucella identification and biotyping. (hindawi.com)
  • Brucella melitensis is the species most pathogenic in humans and the species most commonly involved in ovine and caprine brucellosis. (cdc.gov)
  • Generally, brucellosis is associated with persons who are occupationally in contact with Brucella spp. (cdc.gov)
  • These data illustrate a trend of human brucellosis threatening theoretically low-risk persons in an urban setting and suggest a need for eradicating or controlling Brucella spp. (cdc.gov)
  • Serological and Nucleic Acid Based Detection of Brucellosis in Livestock Species and Molecular Characterization of Brucella melitensis Strains Isolated from Pakistan. (thefreelibrary.com)
  • Brucellosis is a zoonosis of global socio-economic importance caused by Gram negative facultative intracellular bacteria of genus Brucella. (thefreelibrary.com)
  • The brucellae are the etiological agents of brucellosis, a worldwide-distributed zoonosis. (jove.com)
  • Brucellosis is a worldwide extended zoonosis caused by Brucella spp. (jove.com)
  • Most of the data is based on sero-epidemiological studies although it is generally accepted that only the isolation of Brucella confirms the presence of brucellosis. (au-ibar.org)
  • Brucellosis is caused by one of the ten species of the genus Brucella , of which only several can cause human disease. (springer.com)
  • Al Dahouk SF, Scholz HC et al (2007) Evaluation of Brucella MLVA typing for human brucellosis. (springer.com)
  • In China, brucellosis is recognized as a reemerging disease mainly caused by Brucella melitensis specie. (frontiersin.org)
  • Brucellosis is caused by intracellular Gram-negative bacteria belonging to Brucella genus. (frontiersin.org)
  • Brucellosis is a contagious zoonotic disease (affects animals and people) caused by a group of bacteria known scientifically as the genus Brucella. (govdelivery.com)
  • SUMMARY Brucellosis caused by Brucella spp. (asm.org)
  • Recently, Brucella melitensis was declared by the Centers for Disease Control and Prevention to be one of three major bioterrorist agents due to the expense required for the treatment of human brucellosis patients. (asm.org)
  • Brucellosis is one of the five common bacterial zoonoses in the world caused by organisms belonging to the genus Brucella , gram-negative, non-spore-forming, facultative, intracellular bacteria ( 39 ). (asm.org)
  • 9-941 (NC_006932), B. suis 1330 (NC_004310), B. canis American Type Culture Collection 23365 (NC_010103), and B. ovis American Type Culture Collection 25840 (NC_009505). (cdc.gov)
  • For host preference, B. melitensis is associated with sheep and goats, B. abortus with cattle, B. suis with pigs, B. ovis with sheep only, and B. canis with dogs (Verger et al. (thefreelibrary.com)
  • The copy number of IS711 varies in the genome of the different Brucella species, ranging from 7 in B. abortus, B. melitensis and B. suis to more than 30 in B. ovis and in Brucella strains isolated from marine mammals. (nih.gov)
  • Brucella abortus (cattle), Brucella melitensis (goat and sheep), Brucella suis (pigs), Brucella canis (dogs), Brucella ovis (sheep), and Brucella neotomae (rodents). (frontiersin.org)
  • The O-polysaccharide of Brucella LPS is critical for virulence of the classical Brucella species ( B. melitensis, B. abortus , B. suis ). (frontiersin.org)
  • Brucella suis affects mainly swine. (govdelivery.com)
  • Brucella melitensis (sheep and goats), B. suis (hogs), B. abortus (cattle), B. ovis (sheep), B. canis (dogs), B. neotomae (wood rats), and B. maris (marine mammals) ( 38 , 80 ). (asm.org)
  • The recent completion of B. melitensis (GenBank NC 003317 and NC 003318 ) ( 44 ), B. suis (GenBank NC 002969 ), and B. abortus ( 142 ) genome sequence projects has provided tremendous information for understanding the mechanisms of Brucella pathogenicity. (asm.org)
  • The availability of the genomic sequences of B. melitensis , B. suis , and B. abortus will provide an opportunity to identify the virulence mechanisms of Brucella at the molecular level that have previously proven highly elusive. (asm.org)
  • However, the lack of genetic definition of these mutants and the virulence of naturally occurring rough species, Brucella ovis and Brucella canis , has confused interpretation. (asm.org)
  • Brucella canis affects dogs. (govdelivery.com)
  • Of 1,065 persons sampled during a house-to-house survey of an area of the Department of Curuzú Cuatiá, Province of Corrientes, Argentina, 21 were seropositive to Brucella canis antibodies by the gel-diffusion test, using a saline-extracted B. ovis surface R antigen. (ajtmh.org)
  • Brucella ovis infection is usually found in countries with intensive sheep farming. (au-ibar.org)
  • These characteristics encourage their evaluation in the natural host as homologous vaccines for the specific prophylaxis of B. ovis infection. (biomedcentral.com)
  • Additionally, due to its serological cross-reactivity with other S Brucella strains, it is banned in countries where B. melitensis has been eradicated, some of which are important sheep producers and where B. ovis infection constitute a serious problem. (biomedcentral.com)
  • Therefore, the development of a new vaccine able to substitute B. melitensis Rev 1 strain in the prophylaxis of B. ovis infection is a matter of great interest. (biomedcentral.com)
  • Exposure to most species of Brucella , such as those associated with certain types of animals, could potentially lead to infection. (cdc.gov)
  • During the eradication of Brucella ovis infection from five large breeding ram flocks of Sub-Carpathia (The Ukraine), the genital organs of 55 rams culled because of seropositivity in the agar-gel precipitation (AGP) test and ELISA were subjected to gross pathological, histopathological and bacteriological examination. (elsevier.com)
  • ovis infection is associated with a high incidence of epididymitis and orchitis, and a general deterioration in seminal quality with secondary morphological abnormalities of the spermatozoa being a prominent feature. (ac.ke)
  • For the serological diagnosis of B. ovis infection the agar gel immunodiffusion test (AGID) was carried out. (bvsalud.org)
  • Based on the risk factor analysis , it is recommended the diagnosis of B. ovis infection prior to purchase of sheep and the periodic cleaning of the facilities on the farm . (bvsalud.org)
  • The objective of this study was to evaluate the relationship between the infection by B. ovis and the sexual function of rams. (biomedcentral.com)
  • The aim of this study was to examine the relationship between B. ovis infection, evaluated through clinical, serological and bacteriological findings, and the sexual function, in rams originated from infected flocks of this region where CE appears enzootic. (biomedcentral.com)
  • Trueperella pyogenes was isolated from a dog and a cat with a mixed infection with Brucella abortus . (hindawi.com)
  • An epidemiologic survey suggested that raw sheep or goat meat might be the source of Brucella melitensis infection. (cdc.gov)
  • When Brucella infection was diagnosed in her son (patient 1), she was hospitalized for suspected Brucella infection ( Technical Appendix Table ). (cdc.gov)
  • To further explore the involvement of this system to conditions analogous to those occurring during infection, pure cultures of B. ovis cells were subjected to environments of pH (5 and 7) for 15, 30, and 45 minutes and oxidative (50mM H2O2) stress, or Spermine NONOate for 60 minutes. (unt.edu)
  • Brucella ovis infection was more readily set up by injection into the teat canal than by i/v or ocular inoculation. (cabi.org)
  • Brucella melitensis primarily affects the reproductive tract of sheep and goats, and B. melitensis infection is characterized by abortion, retained placenta and, to a lesser extent, impaired fertility. (au-ibar.org)
  • Species-specific multiplex PCR for the diagnosis of Brucella ovis, Actinobacillus seminis, and Histophilus somni infection in rams. (bireme.br)
  • In the next few years, MLVA typing will certainly remain an important epidemiological tool for Brucella infection analysis, as it displays a high discriminatory ability and achieves result largely in agreement with WGS-SNP-based typing. (frontiersin.org)
  • However, in situ analysis of Brucella infected macrophages using antibody directed against O-polysaccharide suggested a loss of reactivity of Brucella consistent with the appearance of rough organisms, and a potential contribution to infection. (frontiersin.org)
  • Epididymal masses are commonly found to be sperm granulomas caused by infection with Brucella ovis , which is a major cause of reproductive loss in sheep. (merckvetmanual.com)
  • 5 WBCs per high power field is questionable (WBCs are correlated with B ovis infection). (merckvetmanual.com)
  • may or may not provide cross-protection against infection by heterologous Brucella species, hampering the acceleration of vaccine development. (asm.org)
  • Brucella infection traced to a single brand of unpasteurized, raw camel milk was diagnosed in 19 patients during a four-month period. (porcamandioca.com)
  • Exotic and reportable causes of abortion include Chlamydophila abortus , Brucella melitensis and Salmonella abortus-ovis . (wa.gov.au)
  • fetus, in 4 (3.6%) Salmonella abortus ovis, in 1 (0.9%) Listeria monocytogenes and in 3 (2.7%) E. coli were isolated and identified. (tubitak.gov.tr)
  • After an antigen stimulus with B. ovis cells, splenocytes obtained from all vaccinated mice secreted similar levels of TNF-α and IL12(p40) and remarkably high amounts of IFN-γ, a crucial cytokine in protective immunity against other Brucella species. (biomedcentral.com)
  • Some Brucella species infect certain marine mammals. (cdc.gov)
  • Brucella ovis is a major cause of reproductive failure in rams and it is one of the few well-described Brucella species that is not zoonotic. (elsevier.com)
  • Previous work showed that a B. ovis mutant lacking a species-specific ABC transporter (ΔabcBA) was attenuated in mice and was unable to survive in macrophages. (elsevier.com)
  • The different species of Brucella are genetically very similar, although each has a slightly different host specificity. (wikipedia.org)
  • Hence, the NCBI taxonomy includes most Brucella species under B. melitensis. (wikipedia.org)
  • People may also be infected by inhalation of contaminated dust or aerosols, and as such, the CDC has labeled Brucella species as highly weaponizable. (wikipedia.org)
  • Currently, the genus Brucella comprises 10 species, which are divided according to host specificity and ability to cause chronic infections in human and animals ( 3 , 4 ). (cdc.gov)
  • Most Brucella species are associated primarily with certain hosts, presumably the result of evolutionary adaptation to a successful host. (cdc.gov)
  • I hypothesized that homologous genetic sequences (Bov_1604-1607), which are similarly found among all Brucella species, may regulate survival functions during pathogenesis. (unt.edu)
  • In this study, a custom MALDI-TOF MS reference library was constructed and its performance on identification at species level was evaluated using 75 Brucella spp. (medworm.com)
  • Analysis of mass peak profiles allowed Brucella accurate identification at genus and species level (100%) with no misidentifications. (medworm.com)
  • Genus Brucella is classified into different species based on phenotypic differences and host preferences. (thefreelibrary.com)
  • 1987). However, since 1994, several new Brucella species have been isolated from marine mammals (Foster et al. (thefreelibrary.com)
  • 2008). More recently, several species of Brucella have been isolated from human (Scholz et al. (thefreelibrary.com)
  • As B. microti is the only Brucella species known to live in soil, its distribution, ecology, zoonotic potential, and genomic organization is of particular interest. (biomedcentral.com)
  • The low number of discovered SNPs suggests an unexpectedly high level of genetic homogeneity in this Brucella species. (biomedcentral.com)
  • Al Dahouk SS, Karges W et al (2010) Differential phenotyping of Brucella species using a newly developed semi-automated metabolic system. (springer.com)
  • The aim of this study was to develop a multiplex PCR assay for simultaneous detection of Brucella ovis, Actinobacillus seminis, and Histophilus somni with species-specific primers applied to biological samples for molecular diagnosis of these infections. (bireme.br)
  • The method was highly specific since it did not amplify DNA from other bacterial species that can potentially cause epididymitis in rams as well as species phylogenetically related to B. ovis. (bireme.br)
  • At present, there is no experimental evidence of transposition of IS711, but the occurrence of this element with a high copy number in some species, and the isolation of Brucella strains with "ectopic" copies of IS711 suggested that this IS could still transpose. (nih.gov)
  • This demonstrated that IS711 are active in vivo, specially in Brucella species with a high number of IS711 copies as B. ovis and B. pinnipedialis. (nih.gov)
  • More recently, additional species have been suggested originating from a wide range of hosts, Brucella ceti (cetaceans), Brucella pinnipedialis (seals), Brucella microti (voles), Brucella papionis (baboons), and Brucella vulpis (foxes) ( 10 - 12 ). (frontiersin.org)
  • Lipopolysaccharide-deficient mutants of smooth Brucella species (rough mutants) have been shown to arise spontaneously in culture. (frontiersin.org)
  • On the basis of LPS expression, the classical species can be broken into two broad categories: smooth (S-) or rough (R-). The classification between S- and R-strains is based on the observation of Brucella colonies using the oblique light method ( Henry, 1933 ). (frontiersin.org)
  • There are many other strains and species, including B. ovis and the newly discoveredB. (scribd.com)
  • The genus of Micrococcus was later renamed Brucella, in honor of David Bruce. (wikipedia.org)
  • Brucella is a genus of Gram-negative bacteria, named after David Bruce (1855-1931). (wikipedia.org)
  • The genus Brucella gained numerous new members in recent years. (biomedcentral.com)
  • Despite the high degree of DNA homology within the Brucella genus, molecular typing schemes based on the use of multiple locus variable number of tandem repeats analysis (MLVA) have proven to be able to differentiate unrelated Brucella isolates which could not be differentiated by classical microbiological methods. (springer.com)
  • The Brucella genome contains an insertion sequence (IS) element called IS711 or IS6501, which is specific to the genus. (nih.gov)
  • Antibodies to group 3 OMPs and the 67.0K protein in the sera of 93 and 87%, respectively, of B. ovis-infected rams were found by immunoblotting. (asm.org)
  • Compared with B. ovis-infected rams which had not developed lesions, rams with epididymo-orchitis had antibodies to a larger variety of proteins. (asm.org)
  • Mice vaccinated with the B. ovis mutants developed anti- B. ovis antibodies in serum of the IgG 1 , IgG 2a and IgG 2b subclasses and their levels were higher than those observed in Rev 1-vaccinated mice. (biomedcentral.com)
  • Two-hundred eighteen sexually-mature rams, from 11 seropositive flocks, were submitted to a clinical examination of the genital tract, a semen collection by electro-ejaculation for spermogram and culture, and a serological examination for anti- B. ovis antibodies by complement fixation test (CFT) and indirect ELISA (I-ELISA). (biomedcentral.com)
  • Detection of antibodies to Brucella ovis by ELISA. (tamu.edu)
  • In the serological examination of 650 sheep and goat blood samples, antibodies were detected in 77 (11.8%) to brucella, 13 (2.0%) to compylobacter and to salmonella in 13 (2.0%) to salmonella, but these sera were found negative for listeria. (tubitak.gov.tr)
  • The possible influence of light was excluded by keeping animals in complete darkness, proving that the external temperature was responsible for this variation 7 .A corresponding variation in antibodies against Brucella abortus to that in normally occurring antibodies could be demonstrated 17 . (scielo.org.za)
  • however, the 1.5-Mb difference in size can be attributed to the acquisition of novel islands in O. anthropi and the degradation of a genome for the brucellae, as has been previously described ( 17 ). (asm.org)
  • The Brucella genome consists of two circular chromosomes without plasmids, suggesting a remarkable difference compared to the single chromosome of many bacteria ( 105 , 106 ). (asm.org)
  • The aim of this study was to evaluate the role of this ABC transporter during intracellular survival of B. ovis. (elsevier.com)
  • The role of the outer membrane proteins of the Omp25/Omp31 family in invasiveness and intracellular survival of virulent B. ovis in phagocytes was analyzed. (biomedsearch.com)
  • Future studies to define the role of these secretion systems in Brucella intracellular survival will be necessary before we can understand the mechanisms of Brucella pathogenesis. (asm.org)
  • Brucella ovis is a sexually transmitted, facultatively anaerobic, intracellular bacterial pathogen of sheep (Ovis aries) and red deer (Cervus elaphus). (unt.edu)
  • O. anthropi , a close relative of brucellae, has not garnered much attention ( 5 , 8 ) but is becoming increasingly recognized as a potentially problematic opportunistic and nosocomial pathogen ( 13 ). (asm.org)
  • Although Brucella is a serious pathogen, type I, II, and III secretion systems and pathogenicity islands were absent. (asm.org)
  • Brucella ovis and Brucella melitensis affect sheep and goats. (govdelivery.com)
  • Both animals lived on a dairy farm with recurrent cases of abortion and mastitis due to Brucella (B.) abortus biovar (bv) 1 infections [ 7 ]. (hindawi.com)
  • By contrast, IL-1α -an enhancer of T cell responses to antigen- was present at higher levels in mice vaccinated with the B. ovis mutants, while IL-10, an anti-inflammatory cytokine, was significantly more abundant in Rev 1-vaccinated mice. (biomedcentral.com)
  • Al-Shamahy HA, Wright SG (1998) Enzyme-linked immunosorbent assay for brucella antigen detection in human sera. (springer.com)
  • The O antigen of Brucella abortus has been described as a major virulence determinant based on the attenuated survival of fortuitously isolated rough variants. (asm.org)
  • Rough Brucella organisms are characterized by a defect in O-antigen presentation and arise spontaneously in cultures in a process classically referred to as dissociation or, perhaps more appropriately, smooth-to-rough phase variation ( 6 , 25 ). (asm.org)
  • Phase variation in Brucella is a reversible phenomenon, and the accumulation of rough organisms in culture suggests that O-antigen expression is not essential under these conditions and is metabolically demanding. (asm.org)
  • Understanding of the role of O antigen in the resistance of Brucella to killing activities is based on experiments using fortuitously isolated rough variants and, as a result, is characterized by a lack of genetic definition. (asm.org)
  • The results of these examinations, as well as the properties of B. ovis strains isolated for the first time in the region are reported. (elsevier.com)
  • Adsorption of immune sera with whole bacteria suggested that group 3 OMPs and 67.0K, 22.5K to 21.5K, and 19.5K to 18.0K proteins had antigenic determinants exposed on the surfaces of both B. ovis and rough B. melitensis cells but not on smooth B. melitensis cells. (asm.org)
  • Because brucellae are often transmitted by direct contact or exposure to a contaminated environment, it is possible that rather than being a natural reservoir for the bacteria, takins are infected horizontally by contact with birth exudates from other infected animals ( 2 ). (cdc.gov)
  • Descubrimos que algunas enzimas del metabolismo de flavinas constituyen factores de virulencia que actúan en el nicho replicativo de la bacteria, y que Brucella tiene un metabolismo de síntesis de flavinas atípico. (leloir.org.ar)
  • Estudiamos además una flavoproteína de Brucella, LOV-HK, que es capaz de sensar la luz azul y de esa manera modula la virulencia de la bacteria. (leloir.org.ar)
  • In western Canada the principle bacteria implicated are Brucella ovis and Actinobacillus seminis . (gov.on.ca)
  • O. anthropi es una bacteria emergente en infecciones intrahospitalarias con notable resistencia antimicrobiana, y es un patógeno inusual en humanos.A case report of bacteraemia by Ochrobactrum anthropi probably associated with bacterial hepatitis, in a inmuno competent patient, who was admitted to the hospital with the diagnostic impression of cholangitis and biliary obstruction. (worldwidescience.org)
  • The most common causative agents of these infections are Brucella ovis, Actinobacillus seminis, and Histophilus somni. (bireme.br)
  • Antisera against Actinobacillus seminis, Brucella ovis and Corynebacterium pseudotuberculosis were prepared in adult female goats. (bmj.com)
  • The aim of this study was the identification of Brucella ovis REO 198 strain pathogenic effect in rams experimentally infected. (bvsalud.org)
  • The mechanisms by which brucellae manifest their pathogenic features are complex. (springer.com)
  • These reports, together with the scant number of studies and the organism's phylogenetic proximity to the highly pathogenic brucellae, make O. anthropi an attractive model of bacterial pathogenicity. (asm.org)
  • The increasing relevance of O. anthropi to human health, together with its phylogenetic proximity to the highly pathogenic brucellae (a USDA Select Agent), prompted us to determine the genomic sequence of the type strain O. anthropi ATCC 49188. (asm.org)
  • Brucella melitensis (B. melitensis) is an infectious bacterial disease that can affect most domestic animals, but goats and sheep are especially susceptible. (ca.gov)
  • These findings demonstrate that Omp25d and Omp22 are essential for the invasion and survival of B. ovis inside host cells, and justify the strong attenuation in virulence of the Deltaomp25d and Deltaomp22 mutants. (biomedsearch.com)
  • These observations show that as WadC, WadB is also involved in the assembly of a branch of Brucella LPS core and support the concept that this LPS section is a virulence-related structure. (jove.com)
  • Brucellae have been found in wildlife, such as bison, elk, and wild boar, potentially posing a threat for zoonosis ( 2 ). (cdc.gov)
  • RESULTS: The multiplex assay was capable of detecting B. ovis, A. seminis, and H. somni DNA simultaneously from genomic bacterial DNA samples and pool of semen samples from experimentally infected rams. (bireme.br)
  • Specific immunofluorescence was observed in cultural smears of A seminis, B ovis and C pseudotuberculosis by the direct technique and in smears of A seminis also by the indirect technique. (bmj.com)
  • Clapp (1955) showed the complement fixation test (C.F.T.) for Brucella ovis in sheep to be well correlated to bacteriological findings in cases where lesions were minimal or absent. (sciquest.org.nz)
  • Brucella ovis causes ram contagious epididymitis, a disease for which a specific vaccine is lacking. (biomedcentral.com)
  • Contagious Epididymitis (CE) due to Brucella ovis ( B. ovis ) is a contagious disease that impairs rams' fertility due to epididymis, testicle and accessory sexual gland alterations. (biomedcentral.com)
  • Simmons and Hall, 1953), and the organism was named Brucella ovis in 1956 (Buddel, 1956). (au-ibar.org)
  • The objective of the present study was the adaptation and evaluation of PCR for B. ovis in semen, urine and organs samples collected from experimentally inoculated animals. (scielo.br)
  • PCR sensitivity for semen, urine and organ samples was analyzed by means of a concentrated B. ovis suspension at 10 -1 , according to McFarland scale. (scielo.br)
  • The incidence of Brucella ovis in some Kenya flocks and its relationship to clinical lesions and semen quality. (ac.ke)
  • This study aimed at molecular detection and characterization of Brucella spp from Pakistan. (thefreelibrary.com)
  • The blood culture positive bacterial isolations were further subjected to classical biotyping and molecular techniques for characterization and found as non-vaccine strains of Brucella melitensis. (thefreelibrary.com)
  • Both chromosomes are highly similar to and colinear with the two chromosomes of Brucella spp. (asm.org)
  • In this study we obtained evidence of transposition of IS711 from the B. ovis and B. pinnipedialis chromosomes by using the "transposon trap" plasmid pGBG1. (nih.gov)
  • Additionally, conditions required for an effective Brucella vaccine strain as well as the future research direction needed to investigate Brucella pathogenesis and host immunity are postulated. (asm.org)
  • Brucella microti was first isolated from common vole ( Microtus arvalis ) in the Czech Republic in Central Europe in 2007. (biomedcentral.com)
  • The B. microti isolate was cultured, after enrichment in Brucella -selective broth, from the submandibular lymph node of a female wild boar that was taken by hunters in Hungary near the Austrian border in September 2014. (biomedcentral.com)
  • Humans are not affected by B. ovis. (au-ibar.org)
  • Disease in Humans B. ovis does not infect humans. (slideserve.com)
  • After exposure to Brucella, humans generally have a two- to four-week latency period before exhibiting symptoms, which include acute undulating fever (>90% of all cases), headache, arthralgia (>50%), night sweats, fatigue, and anorexia. (wikipedia.org)
  • All consignors have either ELISA tested their rams for epididymitis or the rams originate from certified Brucella ovis "free" herds. (wylr.net)
  • MLVA-16 discerned a total of 57 MLVA-16 genotypes among these Brucella strains, with 41 genotypes being firstly detected and the other 16 genotypes being previously reported. (frontiersin.org)
  • Brucella abortus primarily affects cattle, bison, deer and elk. (govdelivery.com)
  • O objetivo do estudo foi adaptar e avaliar a PCR para detecção de Brucella ovis e comparar os resultados com aqueles obtidos por cultivo microbiológico do sêmen, urina e dos órgãos de carneiros infectados experimentalmente. (scielo.br)
  • O trabalho teve como objetivo estudar a patogenia da Brucella ovis, em carneiros infectados experimentalmente com a cepa REO 198, tendo por base a presença do agente por meio da Reação em Cadeia pela Polimerase (PCR) e das alterações anatomopatológicas. (bvsalud.org)
  • No treatment is certain to eliminate Brucella infections. (govdelivery.com)
  • An additional four unrelated isolates were sequenced and used as outliers, including one from camel milk and two from patients with Brucella melitensis with no camel milk exposure. (porcamandioca.com)
  • Demonstration of IS711 transposition in Brucella ovis and Brucella pinnipedialis. (nih.gov)
  • The strains B. melitensis 16 M, B. abortus RB51, B. ovis BOC22 (field strain) and B. pinnipedialis B2/94, all containing the plasmid pGBG1, were grown in culture media with tetracycline until the appearance of tetracycline resistant mutants (TcR). (nih.gov)
  • TcR mutants due to IS711 transposition were only detected in B. ovis and B. pinnipedialis strains. (nih.gov)
  • Six different restriction patterns were observed, four in B. ovis BOC22 (C, D, E and F) and two in B. pinnipedialis B2/94 (A and B, Fig. 3A). (nih.gov)
  • Currently, these investigators ( 44 ) are evaluating the proteomic expression of Brucella to identify the bacterial proteins expressed in vitro. (asm.org)
  • The aim of this investigation was to determine the seroprevalence of Brucella ovis in sheep flocks and individual sheep in the Sertão mesorregion, Paraíba state , Northeastern Brazil , as well as to identify risk factors . (bvsalud.org)
  • Brucella ovis is a Gram-negative coccobacillus from the Brucellaceae family. (wikipedia.org)
  • Brucella melitensis is a Gram-negative coccobacillus bacterium from the Brucellaceae family. (wikipedia.org)
  • In this work, two previously characterized B. ovis attenuated mutants (Δ omp25 d and Δ omp22 ) were evaluated in mice, in comparison with B. melitensis Rev 1, as vaccines against B. ovis . (biomedcentral.com)
  • In some countries vaccines are used to protect against B. ovis with some success, but with unwanted side-effects. (gov.on.ca)
  • No other vaccines are available for other animals, and a human Brucella vaccine does not exist. (asm.org)
  • Crystallization and initial X-ray diffraction analysis of the multi-domain Brucella blue light-activated histidine kinase LOV-HK in its illuminated state. (leloir.org.ar)
  • Three out of the 26 B. ovis strains were derived from AGP-positive but ELISA-negative, while 5 from ELISA-positive but AGP-negative rams. (elsevier.com)
  • The remaining B. ovis strains were isolated from the genital organs of rams found seropositive both by the AGP test and by ELISA. (elsevier.com)
  • Hot saline extracts of Brucella ovis were composed of vesicles with outer membrane proteins (OMPs), lipopolysaccharide, and phospholipid as constituents. (asm.org)
  • The ΔabcBA mutant showed a reduced abundance of the Type IV secretion system (T4SS) proteins VirB8 and VirB11 in both rich and acid media, when compared to WT B. ovis. (elsevier.com)
  • O presente estudo teve por objetivo desenvolver protocolos de PCR em tempo real para B. ovis e H. somni e avaliar sua aplicabilidade em amostras de sêmen e urina de carneiros . (bvsalud.org)
  • Also rams need to scrapie genotype ARR/ARR (the most resistant genotype for scrapie) and be blood tested and proved negative for Brucella ovis before export. (frenchentree.com)
  • According to nucleotide-nucleotide GenBank search by using BLAST ( http://blast.ncbi.nlm.nih.gov/ ), the sequence was 100% identical to the sequences of 16S rDNA of brucellae, especially reference strains including B. melitensis 16M (GenBank accession no. (cdc.gov)
  • However, the underlying molecular mechanism for phase variation in Brucella has not been defined. (asm.org)