Brucella ovis: A species of the genus BRUCELLA which are pathogenic to SHEEP.Brucella: A genus of gram-negative, aerobic bacteria that causes BRUCELLOSIS. Its cells are nonmotile coccobacilli and are animal parasites and pathogens. The bacterium is transmissible to humans through contact with infected dairy products or tissue.Brucellosis: Infection caused by bacteria of the genus BRUCELLA mainly involving the MONONUCLEAR PHAGOCYTE SYSTEM. This condition is characterized by fever, weakness, malaise, and weight loss.Brucella abortus: A species of the genus BRUCELLA whose natural hosts are cattle and other bovidae. Abortion and placentitis are frequently produced in the pregnant animal. Other mammals, including humans, may be infected.Epididymitis: Inflammation of the EPIDIDYMIS. Its clinical features include enlarged epididymis, a swollen SCROTUM; PAIN; PYURIA; and FEVER. It is usually related to infections in the URINARY TRACT, which likely spread to the EPIDIDYMIS through either the VAS DEFERENS or the lymphatics of the SPERMATIC CORD.Brucella melitensis: A species of the genus BRUCELLA whose natural hosts are sheep and goats. Other mammals, including humans, may be infected. In general, these organisms tend to be more virulent for laboratory animals than BRUCELLA ABORTUS and may cause fatal infections.Sheep Diseases: Diseases of domestic and mountain sheep of the genus Ovis.Actinobacillosis: A disease characterized by suppurative and granulomatous lesions in the respiratory tract, upper alimentary tract, skin, kidneys, joints, and other tissues. Actinobacillus lignieresii infects cattle and sheep while A. equuli infects horses and pigs.Brucella Vaccine: A bacterial vaccine for the prevention of brucellosis in man and animal. Brucella abortus vaccine is used for the immunization of cattle, sheep, and goats.Complement Fixation Tests: Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.Sheep: Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS.Bacterial Outer Membrane Proteins: Proteins isolated from the outer membrane of Gram-negative bacteria.Anaplasma ovis: A species of gram-negative bacteria producing mild to severe ANAPLASMOSIS in SHEEP and GOATS, and mild or inapparent infections in DEER and CATTLE.Antigens, Bacterial: Substances elaborated by bacteria that have antigenic activity.Brucella canis: A species of gram-negative bacteria infecting DOGS, the natural hosts, and causing canine BRUCELLOSIS. It can also cause a mild infection in humans.Antibodies, Bacterial: Immunoglobulins produced in a response to BACTERIAL ANTIGENS.Brucellosis, Bovine: A disease of cattle caused by bacteria of the genus BRUCELLA leading to abortion in late pregnancy. BRUCELLA ABORTUS is the primary infective agent.Psoroptidae: Family of parasitic MITES, in the superfamily Sarcoptoidea, order Astigmata. Genera include Psoroptes and Chorioptes.Enzyme-Linked Immunosorbent Assay: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.Sheep, Domestic: A species of sheep, Ovis aries, descended from Near Eastern wild forms, especially mouflon.Biotin: A water-soluble, enzyme co-factor present in minute amounts in every living cell. It occurs mainly bound to proteins or polypeptides and is abundant in liver, kidney, pancreas, yeast, and milk.Pyruvate Carboxylase: A biotin-dependent enzyme belonging to the ligase family that catalyzes the addition of CARBON DIOXIDE to pyruvate. It is occurs in both plants and animals. Deficiency of this enzyme causes severe psychomotor retardation and ACIDOSIS, LACTIC in infants. EC 6.4.1.1.Pimelic Acids: A group of compounds that are derivatives of heptanedioic acid with the general formula R-C7H11O4.Carbon-Nitrogen Ligases: Enzymes that catalyze the joining of two molecules by the formation of a carbon-nitrogen bond. EC 6.3.Acetyl-CoA Carboxylase: A carboxylating enzyme that catalyzes the conversion of ATP, acetyl-CoA, and HCO3- to ADP, orthophosphate, and malonyl-CoA. It is a biotinyl-protein that also catalyzes transcarboxylation. The plant enzyme also carboxylates propanoyl-CoA and butanoyl-CoA (From Enzyme Nomenclature, 1992) EC 6.4.1.2.Animal DiseasesHistoryHepatopancreas: A primitive form of digestive gland found in marine ARTHROPODS, that contains cells similar to those found in the mammalian liver (HEPATOCYTES), and the PANCREAS.Employee Grievances: Formal procedures whereby the employee expresses any dissatisfaction or feeling of injustice regarding the work situation.Health Information Systems: A system for the collection and/or processing of data from various sources, and using the information for policy making and management of health services. It could be paper-based or electronic. (From http://web.worldbank.org/WBSITE/EXTERNAL/TOPICS/EXTHEALTHNUTRITIONANDPOPULATION/EXTHSD/0,,contentMDK:22239824~menuPK:376799~pagePK:148956~piPK:216618~theSitePK:376793,00.html. http://www.who.int/healthinfo/systems/en/)Disease Notification: Notification or reporting by a physician or other health care provider of the occurrence of specified contagious diseases such as tuberculosis and HIV infections to designated public health agencies. The United States system of reporting notifiable diseases evolved from the Quarantine Act of 1878, which authorized the US Public Health Service to collect morbidity data on cholera, smallpox, and yellow fever; each state in the US has its own list of notifiable diseases and depends largely on reporting by the individual health care provider. (From Segen, Dictionary of Modern Medicine, 1992)International Cooperation: The interaction of persons or groups of persons representing various nations in the pursuit of a common goal or interest.Orchitis: Inflammation of a TESTIS. It has many features of EPIDIDYMITIS, such as swollen SCROTUM; PAIN; PYURIA; and FEVER. It is usually related to infections in the URINARY TRACT, which likely spread to the EPIDIDYMIS and then the TESTIS through either the VAS DEFERENS or the lymphatics of the SPERMATIC CORD.Semen: The thick, yellowish-white, viscid fluid secretion of male reproductive organs discharged upon ejaculation. In addition to reproductive organ secretions, it contains SPERMATOZOA and their nutrient plasma.Scrotum: A cutaneous pouch of skin containing the testicles and spermatic cords.AxisTeaching Materials: Instructional materials used in teaching.Pharmacology, Clinical: The branch of pharmacology that deals directly with the effectiveness and safety of drugs in humans.Competitive Behavior: The direct struggle between individuals for environmental necessities or for a common goal.Agriculture: The science, art or practice of cultivating soil, producing crops, and raising livestock.Swine: Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).Video Games: A form of interactive entertainment in which the player controls electronically generated images that appear on a video display screen. This includes video games played in the home on special machines or home computers, and those played in arcades.Fatigue: The state of weariness following a period of exertion, mental or physical, characterized by a decreased capacity for work and reduced efficiency to respond to stimuli.Databases, Protein: Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.Sequence Analysis, Protein: A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.Systems Integration: The procedures involved in combining separately developed modules, components, or subsystems so that they work together as a complete system. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.Proteome: The protein complement of an organism coded for by its genome.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Pyonephrosis: Distention of KIDNEY with the presence of PUS and suppurative destruction of the renal parenchyma. It is often associated with renal obstruction and can lead to total or nearly total loss of renal function.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Foot Bones: The TARSAL BONES; METATARSAL BONES; and PHALANGES OF TOES. The tarsal bones consists of seven bones: CALCANEUS; TALUS; cuboid; navicular; internal; middle; and external cuneiform bones. The five metatarsal bones are numbered one through five, running medial to lateral. There are 14 phalanges in each foot, the great toe has two while the other toes have three each.Erdheim-Chester Disease: A rare form of non-Langerhans-cell histiocytosis (HISTIOCYTOSIS, NON-LANGERHANS-CELL) with onset in middle age. The systemic disease is characterized by infiltration of lipid-laden macrophages, multinucleated giant cells, an inflammatory infiltrate of lymphocytes and histiocytes in the bone marrow, and a generalized sclerosis of the long bones.Myocarditis: Inflammatory processes of the muscular walls of the heart (MYOCARDIUM) which result in injury to the cardiac muscle cells (MYOCYTES, CARDIAC). Manifestations range from subclinical to sudden death (DEATH, SUDDEN). Myocarditis in association with cardiac dysfunction is classified as inflammatory CARDIOMYOPATHY usually caused by INFECTION, autoimmune diseases, or responses to toxic substances. Myocarditis is also a common cause of DILATED CARDIOMYOPATHY and other cardiomyopathies.

Epitope mapping of the Brucella melitensis BP26 immunogenic protein: usefulness for diagnosis of sheep brucellosis. (1/18)

Sequencing of bp26, the gene encoding the Brucella sp. immunogenic BP26 periplasmic protein, was performed in the reference strains of Brucella abortus, B. suis, and B. ovis. The three bp26 sequences were almost identical to that published for B. melitensis 16M bp26, and only minor nucleotide substitutions, without modifying the amino acid sequence, were observed between species. The bp26 genes of the seven B. abortus biovar reference strains and B. abortus S19 and RB51 vaccine strains were also sequenced. Again, only minor differences were found. Surprisingly, the bp26 nucleotide sequence for B. abortus S19 was almost identical to that found for B. melitensis 16M and differed from the sequence described previously by others (O. L. Rossetti, A. I. Arese, M. L. Boschiroli, and S. L. Cravero, J. Clin. Microbiol. 34:165-169, 1996) for the same B. abortus strain. The epitope mapping of BP26, performed by using a panel of monoclonal antibodies and recombinant DNA techniques, allowed the identification of an immunodominant region of the protein interesting for the diagnosis of B. melitensis and B. ovis infection in sheep. A recombinant fusion protein containing this region of BP26 reacted indeed, in Western blotting, as the entire recombinant BP26 against sera from B. melitensis- or B. ovis-infected sheep while it avoided false-positive reactions observed with sera from Brucella-free sheep when using the entire recombinant BP26. Thus, use of this recombinant fusion protein instead the entire recombinant BP26 could improve the specific serological diagnosis of B. melitensis or B. ovis infection in sheep.  (+info)

Lack of evidence of Brucella ovis infection in rams in Quebec. (2/18)

A study was conducted to estimate the seroprevalence of Brucella ovis infection in rams in the Estrie and Bas-Saint-Laurent regions (Quebec). Rams sera (n = 258) were serologically evaluated from 224 rams in 30 commercial flocks and from 34 rams at 2 slaughterhouses by using an enzyme linked immunosorbent assay. Epididymides and testes were examined by palpation on farms and microscopically for culled rams. No ram was seropositive to Brucella ovis or had lesions suggestive of brucellosis from the farm or slaughterhouse surveys.  (+info)

Subcellular fractions of Brucella ovis distinctively induce the production of interleukin-2, interleukin-4, and interferon-gamma in mice. (3/18)

The aim of this study was to evaluate the effect of 3 Brucella ovis subcellular protein fractions: Outer membrane (OMP), inner membrane (IMP), and cytoplasm (CP), on cellular immune response by in vitro production of interleukin (IL)-2, IL-4, and interferon (IFN)-gamma. Each fraction was inoculated 3 times into Balb/c mice, primary cultures of mice spleen cells were done, and these were then stimulated with the fractions. Culture supernatants were collected at 24, 48, 72, 96, and 120 h postinoculation. Cytokine concentration was measured by Duoset-enzyme-linked immunosorbent assay (ELISA). The OMP fraction induced highest cellular immune response of 1000 pg/mL of IL-2 at 24 h, which decreased to < 100 pg/mL by 96 h. The IL-2 response for the IMP fraction was low at 24 h, but exceeded that of the OMP fraction at 72, 96, and 120 h. The CP showed a poor IL response. Regarding the IFN-gamma production, OMP and IMP induced a high response at 120 h. These results open the possibility for the use of B. ovis outer and inner membrane proteins as a subcellular vaccine.  (+info)

A DNA vaccine coding for the Brucella outer membrane protein 31 confers protection against B. melitensis and B. ovis infection by eliciting a specific cytotoxic response. (4/18)

The development of an effective subunit vaccine against brucellosis is a research area of intense interest. The outer membrane proteins (Omps) of Brucella spp. have been extensively characterized as potential immunogenic and protective antigens. This study was conducted to evaluate the immunogenicity and protective efficacy of the B. melitensis Omp31 gene cloned in the pCI plasmid (pCIOmp31). Immunization of BALB/c mice with pCIOmp31 conferred protection against B. ovis and B. melitensis infection. Mice vaccinated with pCIOmp31 developed a very weak humoral response, and in vitro stimulation of their splenocytes with recombinant Omp31 did not induced the secretion of gamma interferon. Splenocytes from Omp31-vaccinated animals induced a specific cytotoxic-T-lymphocyte activity, which leads to the in vitro lysis of Brucella-infected macrophages. pCIOmp31 immunization elicited mainly CD8(+) T cells, which mediate cytotoxicity via perforins, but also CD4(+) T cells, which mediate lysis via the Fas-FasL pathway. In vivo depletion of T-cell subsets showed that the pCIOmp31-induced protection against Brucella infection is mediated predominantly by CD8(+) T cells, although CD4(+)T cells also contribute. Our results demonstrate that the Omp31 DNA vaccine induces cytotoxic responses that have the potential to contribute to protection against Brucella infection. The protective response could be related to the induction of CD8(+) T cells that eliminate Brucella-infected cells via the perforin pathway.  (+info)

Vaccination with the recombinant Brucella outer membrane protein 31 or a derived 27-amino-acid synthetic peptide elicits a CD4+ T helper 1 response that protects against Brucella melitensis infection. (5/18)

The immunogenicity and protective efficacy of the recombinant 31-kDa outer membrane protein from Brucella melitensis (rOmp31), administered with incomplete Freund's adjuvant, were evaluated in mice. Immunization of BALB/c mice with rOmp31 conferred protection against B. ovis and B. melitensis infection. rOmp31 induced a vigorous immunoglobulin G (IgG) response, with higher IgG1 than IgG2 titers. In addition, spleen cells from rOmp31-immunized mice produced interleukin 2 (IL-2) and gamma interferon, but not IL-10 or IL-4, after in vitro stimulation with rOmp31, suggesting the induction of a T helper 1 (Th1) response. Splenocytes from rOmp31-vaccinated animals also induced a specific cytotoxic-T-lymphocyte activity, which led to the in vitro lysis of Brucella-infected macrophages. In vitro T-cell subset depletion indicated that rOmp31 immunization elicited specific CD4+ T cells that secrete IL-2 and gamma interferon, while CD8+ T cells induced cytotoxic-T-lymphocyte activity. In vivo depletion of T-cell subsets showed that the rOmp31-elicited protection against B. melitensis infection is mediated by CD4+ T cells while the contribution of CD8+ T cells may be limited. We then evaluated the immunogenicity and protective efficacy of a known exposed region from Omp31 on the Brucella membrane, a peptide that contains amino acids 48 to 74 of Omp31. Immunization with the synthetic peptide in adjuvant did not elicit a specific humoral response but elicited a Th1 response mediated by CD4+ T cells. The peptide in adjuvant induced levels of protection similar to those induced by rOmp31 against B. melitensis but less protection than was induced by rOmp31 against B. ovis. Our results indicate that rOmp31 could be a useful candidate for the development of subunit vaccines against B. melitensis and B. ovis.  (+info)

CGHScan: finding variable regions using high-density microarray comparative genomic hybridization data. (6/18)

BACKGROUND: Comparative genomic hybridization can rapidly identify chromosomal regions that vary between organisms and tissues. This technique has been applied to detecting differences between normal and cancerous tissues in eukaryotes as well as genomic variability in microbial strains and species. The density of oligonucleotide probes available on current microarray platforms is particularly well-suited for comparisons of organisms with smaller genomes like bacteria and yeast where an entire genome can be assayed on a single microarray with high resolution. Available methods for analyzing these experiments typically confine analyses to data from pre-defined annotated genome features, such as entire genes. Many of these methods are ill suited for datasets with the number of measurements typical of high-density microarrays. RESULTS: We present an algorithm for analyzing microarray hybridization data to aid identification of regions that vary between an unsequenced genome and a sequenced reference genome. The program, CGHScan, uses an iterative random walk approach integrating multi-layered significance testing to detect these regions from comparative genomic hybridization data. The algorithm tolerates a high level of noise in measurements of individual probe intensities and is relatively insensitive to the choice of method for normalizing probe intensity values and identifying probes that differ between samples. When applied to comparative genomic hybridization data from a published experiment, CGHScan identified eight of nine known deletions in a Brucella ovis strain as compared to Brucella melitensis. The same result was obtained using two different normalization methods and two different scores to classify data for individual probes as representing conserved or variable genomic regions. The undetected region is a small (58 base pair) deletion that is below the resolution of CGHScan given the array design employed in the study. CONCLUSION: CGHScan is an effective tool for analyzing comparative genomic hybridization data from high-density microarrays. The algorithm is capable of accurately identifying known variable regions and is tolerant of high noise and varying methods of data preprocessing. Statistical analysis is used to define each variable region providing a robust and reliable method for rapid identification of genomic differences independent of annotated gene boundaries.  (+info)

Improved immunogenicity of a vaccination regimen combining a DNA vaccine encoding Brucella melitensis outer membrane protein 31 (Omp31) and recombinant Omp31 boosting. (7/18)

In the present study, we report an attempt to improve the immunogenicity of the Omp31 antigen by a DNA prime-protein boost immunization regimen. We immunized BALB/c mice with an Omp31 DNA vaccine (pCIOmp31) followed by boosting with recombinant Omp31 (rOmp31) in incomplete Freund's adjuvant and characterized the resulting immune responses and the protective efficacy against Brucella ovis and B. melitensis infection. Immunoglobulin G1 (IgG1) and IgG2a titers were higher in sera from pCIOmp31/rOmp31-immunized mice than in sera from mice immunized with pCIOmp31 or rOmp31 alone. Splenocytes from pCIOmp31/rOmp31-immunized mice produced significantly higher levels of gamma interferon than did those from mice given rOmp31 alone. In contrast, interleukin 2 (IL-2) production levels were comparable between the two groups of immunized mice. Cells from all immunized mice produced undetectable levels of IL-4. Notably, rOmp31 stimulated IL-10 production in the pCIOmp31/rOmp31-immunized group but not in the pCIOmp31- or rOmp31-immunized group. Although the prime-boost regimen induced specific cytotoxic responses, these responses could not reach the levels achieved by the pCIOmp31 immunization. In conclusion, pCIOmp31 priming followed by rOmp31 boosting led to moderately improved protection against a challenge with B. ovis or B. melitensis.  (+info)

Role of the Omp25/Omp31 family in outer membrane properties and virulence of Brucella ovis. (8/18)

The genes coding for the five outer membrane proteins (OMPs) of the Omp25/Omp31 family expected to be located in the outer membrane (OM) of rough virulent Brucella ovis PA were inactivated to evaluate their role in virulence and OM properties. The OM properties of the mutant strains and of the mutants complemented with the corresponding wild-type genes were analyzed, in comparison with the parental strain and rough B. abortus RB51, in several tests: (i) binding of anti-Omp25 and anti-Omp31 monoclonal antibodies, (ii) autoagglutination of bacterial suspensions, and (iii) assessment of susceptibility to polymyxin B, sodium deoxycholate, hydrogen peroxide, and nonimmune ram serum. A tight balance of the members of the Omp25/Omp31 family was seen to be essential for the stability of the B. ovis OM, and important differences between the OMs of B. ovis PA and B. abortus RB51 rough strains were observed. Regarding virulence, the absence of Omp25d and Omp22 from the OM of B. ovis PA led to a drastic reduction in spleen colonization in mice. While the greater susceptibility of the Deltaomp22 mutant to nonimmune serum and its difficulty in surviving in the stationary phase might be on the basis of its dramatic attenuation, no defects in the OM able to explain the attenuation of the Deltaomp25d mutant were found, especially considering that the fully virulent Deltaomp25c mutant displayed more important OM defects. Accordingly, Omp25d, and perhaps Omp22, could be directly involved in the penetration and/or survival of B. ovis inside host cells. This aspect, together with the role of Omp25d and Omp22 in the virulence both of B. ovis in rams and of other Brucella species, should be thoroughly evaluated in future studies.  (+info)

Three flocks (all Romney Marsh) out of 13 were found to be infected, suggesting the disease had been introduced through sheep importations from New Zealand. A study of clinical lesions in the genital tract and of seminal quality made in these and in five of the uninfected flocks showed that Br. ovis infection is associated with a high incidence of epididymitis and orchitis, and a general deterioration in seminal quality with secondary morphological abnormalities of the spermatozoa being a prominent feature ...
Although there has been much written about the phenotype of rough mutants, those reports have relied upon the characterization of fortuitous rough isolates. Since nothing was known about the genetic defects in those organisms, any biological properties attributed to the lack of O antigen influenced the interpretation of subsequent experimental observations. For example, Brucella ovis andB. canis are naturally occurring rough species which are pathogenic in their preferred hosts and resistant to complement-mediated lysis (10, 46). As a result, most researchers have maintained that these organisms must express a shortened (somehow undetectable) O antigen (43). However, recent work in our laboratory with the broadly reactive anti-lipid A antibody (MAb 177) described in this paper indicates the presence of LPS in B. ovis and B. canis which is indistinguishable from that observed in the lps mutants (CA353, CA533, and CA613) of B. abortus (data not shown). The differences in phenotype (virulent versus ...
Moraxella ovis strain ATCC 33078 MovC (movC), RTX A toxin (movA), MovB (movB), MovD (movD), and putative secretion accessory protein (tolC) genes, complete ...
Biotin (vitamin H or vitamin B7) is the essential cofactor of biotin-dependent carboxylases, such as pyruvate carboxylase and acetyl-CoA carboxylase. Mammals cannot synthesize biotin, while in bacteria, fungi, and plants it is synthesized from pimelate thioester through different pathways. In E. coli and many organisms, pimelate thioester is derived from malonyl-ACP. The pathway starts with the methylation to malonyl-ACP methyl ester, followed by the fatty acid chain elongation cycle to form pimeloyl-ACP methyl ester, which is then demethylated to form pimeloyl-ACP [MD:M00572]. Pimeloyl-ACP is converted to biotin through the final four steps in the biotin bicyclic ring assembly, which are conserved among biotin-producing organisms [MD:M00123]. In B. subtilis, biotin is derived from pimeloyl-ACP formed by oxidative cleavage of long-chain acyl-ACPs [MD:M00573]. Some bacteria synthesize biotin from pimeloyl-CoA derived from pimelate [MD:M00577]. Biotin is covalently attached to biotin-dependent ...
If youre a athletics fan, you might have heard of growth hormone - more commonly known as HGH - as well as associate the idea with shady and steroid use. Nonetheless did you know that HUMAN GROWTH HORMONE is a all-natural testosterone booster-style thats produced on its own and offers many crucial benefits? It can true. Hgh is naturally created in the pituitary gland and also plays a huge role in mobile regeneration, progress and keeping healthy human being tissue, like that of the brain and several vital bodily organs. Once produced, HGH is still active in the system for a few short minutes, allowing adequate time for the particular liver for converting it in to growth elements, the most crucial getting insulin-like growing factor (IGF-1), which has growth-promoting properties in each cell in the body. Information taken from www.steroidsonline.org/hgh-for-sale/ ...
Antisera against Actinobacillus seminis, Brucella ovis and Corynebacterium pseudotuberculosis were prepared in adult female goats. Specific immunofluorescence was observed in cultural smears of A seminis, B ovis and C pseudotuberculosis by the direct technique and in smears of A seminis also by the indirect technique. Individual organisms could be recognised. Specific fluorescence of A seminis was readily detected in semen. The results indicate that immunofluorescence may offer an effective method for rapidly and accurately diagnosing bacterial epididymitis in sheep, especially before epididymal lesions are palpable.. ...
Looking for online definition of Neisseria ovis in the Medical Dictionary? Neisseria ovis explanation free. What is Neisseria ovis? Meaning of Neisseria ovis medical term. What does Neisseria ovis mean?
Efecto del uso de medio secuencial humano en la producción de blastocistos de hembra ovis canadensis mexicana por clonación manual
The primers for the genes of the identified proteins were designed and amplificated by PCR in four Brucella strains. The optimized PCR conditions and the length of PCR products were determined. The results of the PCR amplification in four different Brucella strains were shown." +", Corresponding sequence have been able to amplify; "-", Corresponding sequence not been able to amplify ...
When and how often laboratory tests are done may depend on many factors. The timing of laboratory tests may rely on the results or completion of other tests, procedures, or treatments. Lab tests may be performed immediately in an emergency, or tests may be delayed as a condition is treated or monitored. A test may be suggested or become necessary when certain signs or symptoms appear. Due to changes in the way your body naturally functions through the course of a day, lab tests may need to be performed at a certain time of day. If you have prepared for a test by changing your food or fluid intake, lab tests may be timed in accordance with those changes. Timing of tests may be based on increased and decreased levels of medications, drugs or other substances in the body. The age or gender of the person being tested may affect when and how often a lab test is required. Chronic or progressive conditions may need ongoing monitoring through the use of lab tests. Conditions that worsen and improve may ...
Looking for online definition of Ovis aries in the Medical Dictionary? Ovis aries explanation free. What is Ovis aries? Meaning of Ovis aries medical term. What does Ovis aries mean?
Pathogens infecting mammalian cells have developed various strategies to suppress and evade their hosts defensive mechanisms. In this line, the intracellular bacteria that are able to survive and propagate within their host cells must have developed strategies to avert their hosts killing attitude. Studying the interface of host-pathogen confrontation can provide valuable information for defining therapeutic approaches. Brucellosis, caused by the Brucella strains, is a zoonotic bacterial disease that affects thousands of humansand animals around the world inflicting discomfort and huge economic losses. Similar to many other intracellular dwelling bacteria, infections caused by Brucella are difficult to treat, and hence any attempt at identifying new and common therapeutic targets would prove beneficial for the purpose of curing infections caused by the intracellular bacteria. In THP-1 macrophage infected with Brucella melitensis we studied the expression levels of four hosts genes, i.e. EMP2, ST8SIA4
LAA049Ov81, FITC-Linked Polyclonal Antibody to Interferon Gamma (IFNg), 干扰素γ(IFNg)多克隆抗体(异硫氰酸荧光素标记), IFN-G; IFG; IFI; INFr; IFN, Immune Interferon | 仅供体外研究使用,不用于临床诊断!请索取进口关税税单及报关单!
Exerts, in vitro, a potent antimicrobial activity. Probably due to an impairment of the function of the respiratory chain and of energy-dependent activities in the inner membrane of susceptible microorganisms (By similarity).
CEA441Ov, ELISA Kit for Luteinizing Hormone (LH), 黄体激素(LH)检测试剂盒(酶联免疫吸附试验法), ICSH; Lutropin; Interstitial Cell Stimulating Hormone | 仅供体外研究使用,不用于临床诊断!请索取进口关税税单及报关单!
Opens the Highlight Feature Bar and highlights feature annotations from the FEATURES table of the record. The Highlight Feature Bar can be used to navigate to and highlight other features and provides links to display the highlighted region separately. Links in the FEATURES table will also highlight the corresponding region of the sequence. More... ...
Zylexis EQ (Parapox Ovis Virus Immunomodulator) is for use in healthy horses 4 months of age or older as an aid in reducing upper respiratory disease caused by equine herpesvirus types 1 and 4.
Brucella BvrR protein: BvrR/BvrS represent the two-component system involved in the regulation of Brucella virulence; amino acid sequence in first source
FUN_05: Use the SSGCID solved structure of Brucella melitensis methionine-tRNA-synthetase (MetRS) to find inhibitors for potential drug development for brucellosis.
Brucella melitensis bacteria cause persistent, intracellular infections in small ruminants as well as in humans, leading to significant morbidity and economic loss worldwide. The majority of experiments on the transcriptional responses of Brucella to conditions inside the host have been performed following invasion of cultured mammalian cells, and do not address gene expression patterns during long-term infection. Here, we examine the application of the previously developed coincidence cloning methodology to recover and characterize B. melitensis RNA from the supramammary lymph node of experimentally-infected goats. Using coincidence cloning, we successfully recovered Brucella RNA from supramammary lymph nodes of B. melitensis-infected goats at both short-term (4 weeks) and long-term (38 weeks) infection time points. Amplified nucleic acid levels were sufficient for analysis of Brucella gene expression patterns by RNA-sequencing, providing evidence of metabolic activity in both the short-term and the
This article refers to the sheep genus. For the species commonly referred to as "sheep", see sheep (Ovis aries). Ovis is a genus of mammals, part of the goat-antelope subfamily of the ruminant family Bovidae. Its five or more highly sociable species are known as sheep. The domestic sheep is one member of the genus, and is thought to be descended from the wild mouflon of central and southwest Asia. Female sheep are called ewes, males are called rams (sometimes also called bucks or tups), and young sheep are called lambs. The adjective applying to sheep is ovine, and the collective term for sheep is flock or mob. The term herd is also occasionally used in this sense. Many specialist terms relating to domestic sheep are used. Sheep are usually stockier than most other bovines, and their horns are usually divergent and curled into a spiral. Sheep have scent glands on their faces and feet. Communication through the scent glands is not well understood, but is thought to be important for sexual ...
Curated}} {{Biorealm Genus}} [[Image:brucella_close_up.JPG,thumb,400px,right,Electron micrograph of B. abortus located inside cisternae of Vero cell. Within the perinuclear envelope, the cisternae containing B. abortus are discontinuously lined by ribosomes (arrows). From [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2114362 Detilleux et al.]]] ==Classification== ===Higher order taxa:=== Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Brucellaceae ===Species:=== Brucella abortus, Brucella canis, Brucella cetaceae, Brucella maris , Brucella melitensis, Brucella pinnipediae, Brucella sp. {, , height="10" bgcolor="#FFDF95" align="center" , NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=234 Taxonomy] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=genomeprj&cmd=Search&dopt=DocSum&term=txid234%5BOrganism:exp%5D Genomes] ,} ==Description and Significance== Brucella sp. causes brucellosis, ...
We present a rare case of brucella endocarditis, forming a vegetation on the mitral valve. The definitive diagnosis has been made with clinical suspicion, positive serology, the demonstration of the vegetation with the echocardiography and with the production from the multiple blood culture of brucella melitensis and from the excised valve. Our patient has been successfully treated with specific antibiotherapy and the surgery of replacement of mitral valve. Our aim in presenting the case is to remind the infective endocarditis which is due to this factor in the regions like our country which is endemic for ...
The Cyprian wild sheep - the national animal of Cyprus - was probably introduced by man from Asia minor to Cyprus about 8000 to 10000 years ago. With an average shoulder height of 66 cm, the Cyprus mouflon is the smallest of all wild sheep. There is a clear sexual dimorphism: males (body-weight 30-40 kg) are larger than the females (body-weight 20-30 kg) and have much larger horns, which may reach a length of 71 cm. ...
BIOSYNTHESE + ANABOLISMUS (MIKROBIOLOGIE); PROTEINBIOSYNTHESE + PROTEINANABOLISMUS + PEPTIDBIOSYNTHESE + PEPTIDANABOLISMUS (METABOLISMUS); PANSENFLORA (TIERPHYSIOLOGIE, TIERMORPHOLOGIE); SCHAFE + SCHAFZUCHT + SCHAFHALTUNG (TIERHALTUNG); OVIS (ZOOLOGIE); HARNCHEMIE (TIERPHYSIOLOGIE); PURINMETABOLISMUS; BIOSYNTHESIS + ANABOLISM (MICROBIOLOGY); PROTEIN BIOSYNTHESIS + PROTEIN ANABOLISM + PEPTIDE BIOSYNTHESIS + PEPTIDE ANABOLISM (METABOLISM); RUMEN FLORA (ANIMAL PHYSIOLOGY, ANIMAL MORPHOLOGY); SHEEP + SHEEP BREEDING + SHEEP HUSBANDRY (ANIMAL HUSBANDRY); OVIS (ZOOLOGY); URINE CHEMISTRY (ANIMAL PHYSIOLOGY); PURINE ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Journal of Wildlife Diseases publishes work on infectious, parasitic, toxic, nutritional, physiologic, and neoplastic diseases impacting wild animals.
PCR-RFLP analysis of Brucella LPS genes manAO-Ag, manBO-Ag, wbkD, wbkF, wboA and wa**. Panel A. Lanes: 1, molecular size markers; 2, manAO-Ag from B. melitensis
Left mandible of sheep (Ovis aries)Photo author: Albert Fischer - Date: 27/04/2014.For more information, refer to the presentation of the corpus of the author. - Ovis-Jaw-Left.jpg
The patterns of susceptibility to hydrophobic and hydrophilic drugs and the uptake of the fluorescent probe N-phenyl-naphthylamine in Brucella spp., Haemophilus influenzae, Escherichia coli, and deep rough Salmonella minnesota mutants were compared. The results show that the outer membranes of smooth and naturally rough Brucella spp. do not represent barriers to hydrophobic permeants and that this absence of a barrier relates at least in part to the properties of Brucella lipopolysaccharide. ...
... in New Zealand is limited to sheep (B. ovis). The country is free of all other species of Brucella. Dairy herds in ... Brucella species survive well in aerosols and resist drying. Brucella and all other remaining biological weapons in the U.S. ... ovis (sheep), and B. suis (caribou and pigs). Brucella species have also been isolated from several marine mammal species ( ... The growth of brucellae is extremely slow (they can take up to two months to grow) and the culture poses a risk to laboratory ...
viciae 3841 strain, and the Agrobacterium species A. vitis,A. tumefaciens, A. radiobacter and A. H13, Brucella species (B. ovis ... as well as in a broad spectrum of Brucella species (B. ovis, B. canis, B. abortus and B. microtis, and several viobars of B. ... Partial synteny of the αr45 genomic regions was observed in the Mesorhizobium and Brucella species where instead of aLysR ... This analysis also revealed an extended conserved sequence stretch among the promoters of the Brucella and Bartonella αr45 sRNA ...
... as well as in a broad spectrum of Brucella species (B. ovis, B. canis, B. abortus and B. microtis, and several viobars of B. ... but lower bit-scores and are encoded by Brucella species (B. ovis, B. canis, B. abortus, B. microtis, and several biobars of B ... The second group is formed by the first copy in the Chromosome I in the Brucellas genomes that share the gene upstream coding ... and the second copy in chromosome I in the Brucella group (Bor14CI2, Bcr14CI2, Bmir14CI2, Bs1330r14CI2, Bm16Mr14CI1, ...
... as well as in a broad spectrum of Brucella species (B. ovis, B. canis, B. abortus and B. microtis, and several viobars of B. ... but lower bit-scores and are encoded by Brucella species (B. ovis, B. canis, B. abortus, B. microtis, and several biobars of B ... A special case is the Brucella group, where primary automatic annotation over their genomes identified ORFs smaller than 30 aa ...
... as well as in a broad spectrum of Brucella species (B. ovis, B. canis, B. abortus and B. microtis, and several biovars of B. ... the plasmidic copies of all mentioned α-proteobacterial genomes and those αr15 members encoded by Brucella species (B. ovis, B ... To the last group correspond the αr15CII loci in the Brucella group (additional file 4) where only one of the genes could be ... The second group includes the αr15CI1 loci in the Brucella species (additional file 2), which presented a very well conserved ...
Brucella suis and other Brucella species (B. melitensis, B. abortis, B. ovis, B. canis) are recognized as potential ... The Brucella abortus (rough LPS Brucella) vaccine, developed for bovine brucellosis and licensed by the USDA Animal Plant ... of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella ... "Brucella abortus cyclic beta-1,2-glucan mutants have reduced virulence in mice and are defective in intracellular replication ...
Brucella ovis ATCC 25840 chromosome I (NC_009505), Bmir7CI = Brucella microti CCM 4915 chromosome 1 (NC_013119), Oar7CI = ... 16M chromosome I (NC_003317), BaS19r7CI = Brucella abortus S19 chromosome 1 (NC_010742), Bm23457r7CI = Brucella melitensis ATCC ... as well as in a broad spectrum of Brucella species (B. ovis, B. canis, B. abortus and B. microtis, and several biobars of B. ... but lower bit-scores and are encoded by Brucella species (B. ovis, B. canis, B. abortus, B. microtis, and several biobars of B ...
Brucella melitensis MeSH B03.440.400.425.215.500.625 --- Brucella ovis MeSH B03.440.400.425.215.500.750 --- Brucella suis MeSH ... Brucella melitensis MeSH B03.660.050.070.100.625 --- Brucella ovis MeSH B03.660.050.070.100.750 --- Brucella suis MeSH B03.660. ... Brucella MeSH B03.440.400.425.215.500.100 --- Brucella abortus MeSH B03.440.400.425.215.500.150 --- Brucella canis MeSH B03.440 ... Brucella MeSH B03.660.050.070.100.100 --- Brucella abortus MeSH B03.660.050.070.100.150 --- Brucella canis MeSH B03.660.050.070 ...
3,279 ORFs Brucella microti CCM 4915, 3,346 ORFs Brucella ovis ATCC 25840, 3,193 ORFs Brucella pinnipedialis B2/94, 3,505 ORFs ... Brucella abortus A13334, 3,401 ORFs Brucella canis ATCC 23365, 3,408 ORFs Brucella melitensis 16M, ... Brucella species have been found primarily in mammals: Pathogenic Brucella species can cause abortion in female animals by ... Conversely, depriving Brucella of the blue wavelengths dropped its reproductive rate by 90%. Parte, A.C. "Brucella". Muleme.M ...
link) Ocampo-Sosa, Alain A; García-Lobo, Juan M (2008). "Demonstration of IS711 transposition in Brucella ovis and Brucella ... and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts". International ... "The genome sequence of Brucella pinnipedialis B2/94 sheds light on the evolutionary history of the genus Brucella". BMC ... Brucella pinnipedialis is a species of bacteria. It causes infections and related diseases primarily in pinnipeds and cetaceans ...
The bacterium causes ovine brucellosis, along with Brucella ovis. It can infect sheep, cattle, and sometimes humans, and it can ... Brucella melitensis genomes and related information at PATRIC, a Bioinformatics Resource Center funded by NIAID Brucella ... It is zoonotic, unlike B. ovis, causing Malta fever or localized brucellosis in humans. The bacterium causes severe ... Brucella melitensis is a Gram-negative coccobacillus bacterium from the Brucellaceae family. ...
... is a Gram-negative coccobacillus from the Brucellaceae family. Along with Brucella melitensis, it is responsible ... Brucella ovis, reviewed and published by Wikivet at http://en.wikivet.net/Brucella_ovis, accessed 24/08/2011.. ... B. ovis can be transmitted by the stable fly. Infection causes severe inflammation of the epididymis, particularly the tail. ...
Elands produce antibodies for Brucella bacteria, but none for Mycobacterium paratuberculosis or various types of pneumonia like ... Ovis. *Argali (O. ammon). *Domestic sheep (O. aries). *Bighorn sheep (O. canadensis) ...
Brucellosis is caused by different biotypes of Brucella abortus and B. melitensis.[52] Other internal parasites include ... Ovis. *Argali (O. ammon). *Domestic sheep (O. aries). *Bighorn sheep (O. canadensis) ...
21 were seropositive to Brucella canis antibodies by the gel-diffusion test, using a saline-extracted B. ovis surface R antigen ... 21 were seropositive to Brucella canis antibodies by the gel-diffusion test, using a saline-extracted B. ovis surface R antigen ... Occurrence of Antibodies to Brucella Canis in Rural Inhabitants of Corrientes and Neuquén Provinces, Argentina * Victor M. ...
Brucella ovis is a Gram-negative coccobacillus from the Brucellaceae family. Along with Brucella melitensis, it is responsible ... Brucella ovis, reviewed and published by Wikivet at http://en.wikivet.net/Brucella_ovis, accessed 24/08/2011.. ... B. ovis can be transmitted by the stable fly. Infection causes severe inflammation of the epididymis, particularly the tail. ...
Biotin metabolism - Brucella ovis [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show description , User data ...
1985)‎. Proposed international standard for anti-brucella ovis serum / The Central Veterinary Laboratory, Weybridge, Surrey, ... Proposed international standard for anti-brucella ovis serum / The Central Veterinary Laboratory, Weybridge, Surrey, United ... Metabolic and bacteriophage identification of Brucella strains described as Brucella melitensis from cattle*  Meyer, Margaret ... The Second International Standard for Anti-Brucella abortus Serum*  Davidson, Ian; Hebert, C. Nancy; Morgan, W. J. Brinley (‎ ...
Brucella ovis ATCC 25840. Mutation(s): 2 Gene Names: galE-1, BOV_A0474. EC: 5.1.3.2. ... Crystal structure of UDP-glucose 4-epimerase from Brucella ovis in complex with NAD. *DOI: 10.2210/pdb4WOK/pdb ... Find proteins for A0A0H3ASR6 (Brucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512)) ... Crystal structure of UDP-glucose 4-epimerase from Brucella ovis in complex with NAD. SSGCID, Seattle Structural Genomics Center ...
... from Brucella ovis in complex with a partially ordered NAD ... Find proteins for A0A0H3AQ75 (Brucella ovis (strain ATCC 25840 ... from Brucella ovis in complex with a partially ordered NAD. Abendroth, J., Dranow, D.M., Lorimer, D.D., Edwards, T.E.. To be ... Crystal structure of an Oxidoreductase (short chain dehydrogenase/reductase family) from Brucella ovis in complex with a ... Brucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512). Mutation(s): 0 ...
Brucella abortus biovar 1 (strain 9-941). Brucella abortus bv. 5 str. B3196. Brucella abortus NCTC 8038. Brucella abortus str. ... Brucella canis (strain ATCC 23365 / NCTC 10854). Brucella melitensis biotype 2 (strain ATCC 23457). Brucella melitensis biotype ... Brucella ovis IntaBari-2002-82-58. 538. UniRef100_A5VSF8. Cluster: Bifunctional purine biosynthesis protein PurH. 2. ... sp,A5VSF8,PUR9_BRUO2 Bifunctional purine biosynthesis protein PurH OS=Brucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512) ...
Brucella ovis Gram negative coccobacillus Facultative intracellular pathogen Slideshow 4806253 by shania ... Brucella ovis. Overview. Organism History Epidemiology Transmission Disease in Animals Prevention and Control Actions to Take. ... Ovine Epididymitis: Brucella ovis. Overview. Organism History Epidemiology Transmission Disease in Animals Prevention and ... The Organism • Brucella ovis • Gram negativecoccobacillus • Facultativeintracellular pathogen • Persists in the environment ...
Antibody response to Brucella ovis outer membrane proteins in ovine brucellosis.. J I Riezu-Boj, I Moriyón, J M Blasco, C ... Antibody response to Brucella ovis outer membrane proteins in ovine brucellosis.. J I Riezu-Boj, I Moriyón, J M Blasco, C ... Antibody response to Brucella ovis outer membrane proteins in ovine brucellosis.. J I Riezu-Boj, I Moriyón, J M Blasco, C ... Antibody response to Brucella ovis outer membrane proteins in ovine brucellosis. Message Subject (Your Name) has forwarded a ...
Brucella ovis) (OPCA101054) , Recombinant Protein , Application: WB, ELISA , Species Reactivity: Brucella ovis , Alias: ...
Find out information about Brucella ovis. A genus of gram-negative, aerobic bacteria of uncertain affiliation; single, ... nonmotile coccobacilli or short rods, all of which are parasites and pathogens... Explanation of Brucella ovis ... Related to Brucella ovis: Brucella abortus, Brucella canis, Brucella melitensis, Brucella neotomae Brucella. [brü′sel·ə] ( ... Brucella ovis , Article about Brucella ovis by The Free Dictionary https://encyclopedia2.thefreedictionary.com/Brucella+ovis ...
Experimental Infection by Brucella ovis: Changes in NTPDase, 5-Nucleotidase and Acetylcholinesterase Associated Cerebral ...
Mice vaccinated with the B. ovis mutants developed anti-B. ovis antibodies in serum of the IgG1, IgG2a and IgG2b subclasses and ... Additionally, the B. ovis mutants showed appropriate persistence, limited splenomegaly and protective efficacy against B. ovis ... ovis infection, but its use for this purpose has serious drawbacks. In this work, two previously characterized B. ovis ... Attenuated Brucella melitensis Rev 1, used as vaccine against ovine and caprine brucellosis caused by B. melitensis, is also ...
Three types of the bacteria that cause brucellosis - Brucella abortus, Brucella melitensis and Brucella suis - are designated ... Exposure to most species of Brucella, such as those associated with certain types of animals, could potentially lead to ... Human Neurobrucellosis with Intracerebral Granuloma Caused by a Marine Mammal Brucella spp. ... Brucella ovis and neotomae. *Not known to be pathogenic for humans. Brucella suis* ...
Dénes B, Glávits R. Bacteriologically confirmed cases of ovine epididymo-orchitis caused by Brucella ovis in Sub-Carpathia. ... Bacteriologically confirmed cases of ovine epididymo-orchitis caused by Brucella ovis in Sub-Carpathia. / Dénes, B.; Glávits, R ... Dénes, B., & Glávits, R. (1994). Bacteriologically confirmed cases of ovine epididymo-orchitis caused by Brucella ovis in Sub- ... Dénes, B. ; Glávits, R. / Bacteriologically confirmed cases of ovine epididymo-orchitis caused by Brucella ovis in Sub- ...
Brucella ovis infection. Caprine and ovine brucellosis (B. melitensis). Caprine arthritis/encephalitis. Contagious agalactia. ...
Ovine epididymitis (Brucella ovis). Present. Peste des petits ruminants. Free. Never occurred ...
Ovine epididymitis (Brucella ovis) *Peste des petits ruminants. *Salmonellosis (S. abortusovis) *Scrapie ...
... ... ovis infection is associated with a high incidence of epididymitis and orchitis, and a general deterioration in seminal quality ...
Some Brucella species infect certain marine mammals. Most humans who get brucellosis get it from dairy animals or dairy ... B. abortus infects domestic livestock (cattle, bison, and elk); B. melitensis, goats and sheep; B. ovis, sheep; and B. suis, ... Brucellosis is a disease caused by Brucella bacteria of several species. Different species tend to infect different animals. ...
Ovine epididymitis (Brucella ovis). *Ovine pulmonary adenomatosis. *Paratuberculosis (Johnes disease). *Porcine reproductive ...
Brucella ovis is a major cause of reproductive failure in rams and it is one of the few well-described Brucella species that is ... N2 - Brucella ovis is a major cause of reproductive failure in rams and it is one of the few well-described Brucella species ... AB - Brucella ovis is a major cause of reproductive failure in rams and it is one of the few well-described Brucella species ... abstract = "Brucella ovis is a major cause of reproductive failure in rams and it is one of the few well-described Brucella ...
Brucella ovis. Brucellosis. by products. calving. canter. caps. care for recumbent patient. ...
Brucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512). Loading... A5W4E3 3D Sensor histidine kinase TodS. Pseudomonas putida ...
Brucella ovis (strain ATCC 25840 / 63/290 / NCTC 10512). Loading... A5WCA0 tRNA(Ile)-lysidine synthase. Psychrobacter sp. ( ...
  • Brucella melitensis Rev 1 is an S live attenuated strain currently used for vaccination campaigns against ovine and caprine brucellosis, which is almost exclusively caused by B. melitensis and B. ovis . (biomedcentral.com)
  • The cultural, morphological and most important biochemical properties of the 26 sub-Carpathian B. ovis strains were identical with those of the reference strain designated Weybridge 63/290 (NCTC 10512). (elsevier.com)
  • Brucella abortus Strain 19 injected into rams by various routes persisted in the seminal vesicles. (cabi.org)
  • The serological differences are related to the amounts of A and M antigens that a Brucella strain possesses. (au-ibar.org)
  • The increasing relevance of O. anthropi to human health, together with its phylogenetic proximity to the highly pathogenic brucellae (a USDA Select Agent), prompted us to determine the genomic sequence of the type strain O. anthropi ATCC 49188. (asm.org)
  • The long-term trial with brucella Strain 19 vaccine showed that there was no advantage from re-vaccination. (cabi.org)
  • The results of these examinations, as well as the properties of B. ovis strains isolated for the first time in the region are reported. (elsevier.com)
  • Brucella microti was first isolated from common vole ( Microtus arvalis ) in the Czech Republic in Central Europe in 2007. (biomedcentral.com)
  • The B. microti isolate was cultured, after enrichment in Brucella -selective broth, from the submandibular lymph node of a female wild boar that was taken by hunters in Hungary near the Austrian border in September 2014. (biomedcentral.com)
  • Brucellosis caused by Brucella ovis mainly induces chronic epididymitis and orchitis in rams. (biomedcentral.com)
  • From the 55 pairs of epididymides and testicles examined, a total of 26 B. ovis strains were isolated: of them, 24 (92.3%) originated from breeding rams while 2 (7.7%) from ram hoggets kept together with the breeding rams. (elsevier.com)
  • Seven out of the 26 B. ovis strains (26.9%) were cultured from the epididymides of rams which did not show palpable epididymal and/or testicular lesions. (elsevier.com)
  • Three out of the 26 B. ovis strains were derived from AGP-positive but ELISA-negative, while 5 from ELISA-positive but AGP-negative rams. (elsevier.com)
  • The remaining B. ovis strains were isolated from the genital organs of rams found seropositive both by the AGP test and by ELISA. (elsevier.com)
  • RESULTS: The multiplex assay was capable of detecting B. ovis, A. seminis, and H. somni DNA simultaneously from genomic bacterial DNA samples and pool of semen samples from experimentally infected rams. (bireme.br)
  • Also rams need to scrapie genotype ARR/ARR (the most resistant genotype for scrapie) and be blood tested and proved negative for Brucella ovis before export. (frenchentree.com)
  • Specific immunofluorescence was observed in cultural smears of A seminis, B ovis and C pseudotuberculosis by the direct technique and in smears of A seminis also by the indirect technique. (bmj.com)
  • According to nucleotide-nucleotide GenBank search by using BLAST ( http://blast.ncbi.nlm.nih.gov/ ), the sequence was 100% identical to the sequences of 16S rDNA of brucellae, especially reference strains including B. melitensis 16M (GenBank accession no. (cdc.gov)
  • Adsorption of immune sera with whole bacteria suggested that group 3 OMPs and 67.0K, 22.5K to 21.5K, and 19.5K to 18.0K proteins had antigenic determinants exposed on the surfaces of both B. ovis and rough B. melitensis cells but not on smooth B. melitensis cells. (asm.org)
  • Brucella strains are smooth (S) or rough (R), depending on the presence or absence of O-polysaccharide (O-PS) chains in the lipopolysaccharide (LPS) respectively. (biomedcentral.com)
  • The isolate could be agglutinated by A-monospecific antiserum but not by M-monospecific antiserum or rough Brucella- specific antiserum. (cdc.gov)
  • Several environmental factors, including nutrient availability, temperature, and aeration, have been shown to influence the rate of appearance of rough Brucella organisms in culture ( 5 ). (asm.org)
  • Experimental evidence suggests that rough Brucella variants would be rapidly destroyed by complement-mediated lysis or by the phagocytic cells of the infected host. (asm.org)
  • Rough mutants of Brucella spp. (asm.org)
  • In this work, two previously characterized B. ovis attenuated mutants (Δ omp25 d and Δ omp22 ) were evaluated in mice, in comparison with B. melitensis Rev 1, as vaccines against B. ovis . (biomedcentral.com)
  • Additionally, the B. ovis mutants showed appropriate persistence, limited splenomegaly and protective efficacy against B. ovis similar to that observed with B. melitensis Rev 1. (biomedcentral.com)
  • These findings demonstrate that Omp25d and Omp22 are essential for the invasion and survival of B. ovis inside host cells, and justify the strong attenuation in virulence of the Deltaomp25d and Deltaomp22 mutants. (biomedsearch.com)
  • Genetic trans-complementation using a plasmid-based expression of Brucella manBA successfully restored O-polysaccharide expression in only one-third of O-polysaccharide deficient spontaneous mutants. (frontiersin.org)
  • Antibody response to Brucella ovis outer membrane proteins in ovine brucellosis. (asm.org)
  • Hot saline extracts of Brucella ovis were composed of vesicles with outer membrane proteins (OMPs), lipopolysaccharide, and phospholipid as constituents. (asm.org)
  • The ΔabcBA mutant showed a reduced abundance of the Type IV secretion system (T4SS) proteins VirB8 and VirB11 in both rich and acid media, when compared to WT B. ovis. (elsevier.com)