Brucella melitensis: A species of the genus BRUCELLA whose natural hosts are sheep and goats. Other mammals, including humans, may be infected. In general, these organisms tend to be more virulent for laboratory animals than BRUCELLA ABORTUS and may cause fatal infections.Brucellosis: Infection caused by bacteria of the genus BRUCELLA mainly involving the MONONUCLEAR PHAGOCYTE SYSTEM. This condition is characterized by fever, weakness, malaise, and weight loss.Brucella: A genus of gram-negative, aerobic bacteria that causes BRUCELLOSIS. Its cells are nonmotile coccobacilli and are animal parasites and pathogens. The bacterium is transmissible to humans through contact with infected dairy products or tissue.Brucella abortus: A species of the genus BRUCELLA whose natural hosts are cattle and other bovidae. Abortion and placentitis are frequently produced in the pregnant animal. Other mammals, including humans, may be infected.Brucella Vaccine: A bacterial vaccine for the prevention of brucellosis in man and animal. Brucella abortus vaccine is used for the immunization of cattle, sheep, and goats.Brucella ovis: A species of the genus BRUCELLA which are pathogenic to SHEEP.Brucella suis: A species of gram-negative bacteria, primarily infecting SWINE, but it can also infect humans, DOGS, and HARES.Sheep Diseases: Diseases of domestic and mountain sheep of the genus Ovis.Brucellosis, Bovine: A disease of cattle caused by bacteria of the genus BRUCELLA leading to abortion in late pregnancy. BRUCELLA ABORTUS is the primary infective agent.Goats: Any of numerous agile, hollow-horned RUMINANTS of the genus Capra, in the family Bovidae, closely related to the SHEEP.Rose Bengal: A bright bluish pink compound that has been used as a dye, biological stain, and diagnostic aid.Goat Diseases: Diseases of the domestic or wild goat of the genus Capra.Brucella canis: A species of gram-negative bacteria infecting DOGS, the natural hosts, and causing canine BRUCELLOSIS. It can also cause a mild infection in humans.Antibodies, Bacterial: Immunoglobulins produced in a response to BACTERIAL ANTIGENS.Doxycycline: A synthetic tetracycline derivative with similar antimicrobial activity.Antigens, Bacterial: Substances elaborated by bacteria that have antigenic activity.Antibodies, Immobilized: Antibodies that are chemically bound to a substrate material which renders their location fixed.Sheep: Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS.Bacterial Proteins: Proteins found in any species of bacterium.Bacterial Outer Membrane Proteins: Proteins isolated from the outer membrane of Gram-negative bacteria.Microbiology: The study of microorganisms such as fungi, bacteria, algae, archaea, and viruses.Agglutination Tests: Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (From Stedman, 26th ed)Mice, Inbred BALB CDNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Streptomycin: An antibiotic produced by the soil actinomycete Streptomyces griseus. It acts by inhibiting the initiation and elongation processes during protein synthesis.Genome, Bacterial: The genetic complement of a BACTERIA as represented in its DNA.Virulence: The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.Sulfur: An element that is a member of the chalcogen family. It has an atomic symbol S, atomic number 16, and atomic weight [32.059; 32.076]. It is found in the amino acids cysteine and methionine.Sulfur Compounds: Inorganic or organic compounds that contain sulfur as an integral part of the molecule.3-Mercaptopropionic Acid: An inhibitor of glutamate decarboxylase. It decreases the GAMMA-AMINOBUTYRIC ACID concentration in the brain, thereby causing convulsions.Sulfonium Compounds: Sulfur compounds in which the sulfur atom is attached to three organic radicals and an electronegative element or radical.Endocarditis, Bacterial: Inflammation of the ENDOCARDIUM caused by BACTERIA that entered the bloodstream. The strains of bacteria vary with predisposing factors, such as CONGENITAL HEART DEFECTS; HEART VALVE DISEASES; HEART VALVE PROSTHESIS IMPLANTATION; or intravenous drug use.Endocarditis: Inflammation of the inner lining of the heart (ENDOCARDIUM), the continuous membrane lining the four chambers and HEART VALVES. It is often caused by microorganisms including bacteria, viruses, fungi, and rickettsiae. Left untreated, endocarditis can damage heart valves and become life-threatening.Bile Acids and Salts: Steroid acids and salts. The primary bile acids are derived from cholesterol in the liver and usually conjugated with glycine or taurine. The secondary bile acids are further modified by bacteria in the intestine. They play an important role in the digestion and absorption of fat. They have also been used pharmacologically, especially in the treatment of gallstones.Lithocholic Acid: A bile acid formed from chenodeoxycholate by bacterial action, usually conjugated with glycine or taurine. It acts as a detergent to solubilize fats for absorption and is itself absorbed. It is used as cholagogue and choleretic.Deoxycholic Acid: A bile acid formed by bacterial action from cholate. It is usually conjugated with glycine or taurine. Deoxycholic acid acts as a detergent to solubilize fats for intestinal absorption, is reabsorbed itself, and is used as a choleretic and detergent.Chenodeoxycholic Acid: A bile acid, usually conjugated with either glycine or taurine. It acts as a detergent to solubilize fats for intestinal absorption and is reabsorbed by the small intestine. It is used as cholagogue, a choleretic laxative, and to prevent or dissolve gallstones.Cholic Acids: The 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholanic acid family of bile acids in man, usually conjugated with glycine or taurine. They act as detergents to solubilize fats for intestinal absorption, are reabsorbed by the small intestine, and are used as cholagogues and choleretics.Bile: An emulsifying agent produced in the LIVER and secreted into the DUODENUM. Its composition includes BILE ACIDS AND SALTS; CHOLESTEROL; and ELECTROLYTES. It aids DIGESTION of fats in the duodenum.Deoxyribonucleases, Type II Site-Specific: Enzyme systems containing a single subunit and requiring only magnesium for endonucleolytic activity. The corresponding modification methylases are separate enzymes. The systems recognize specific short DNA sequences and cleave either within, or at a short specific distance from, the recognition sequence to give specific double-stranded fragments with terminal 5'-phosphates. Enzymes from different microorganisms with the same specificity are called isoschizomers. EC 3.1.21.4.Bacteriology: The study of the structure, growth, function, genetics, and reproduction of bacteria, and BACTERIAL INFECTIONS.Agrobacterium tumefaciens: A species of gram-negative, aerobic bacteria isolated from soil and the stems, leafs, and roots of plants. Some biotypes are pathogenic and cause the formation of PLANT TUMORS in a wide variety of higher plants. The species is a major research tool in biotechnology.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.Rhizobium: A genus of gram-negative, aerobic, rod-shaped bacteria that activate PLANT ROOT NODULATION in leguminous plants. Members of this genus are nitrogen-fixing and common soil inhabitants.Plant Tumors: A localized proliferation of plant tissue forming a swelling or outgrowth, commonly with a characteristic shape and unlike any organ of the normal plant. Plant tumors or galls usually form in response to the action of a pathogen or a pest. (Holliday, P., A Dictionary of Plant Pathology, 1989, p330)Ciprofloxacin: A broad-spectrum antimicrobial carboxyfluoroquinoline.Ofloxacin: A synthetic fluoroquinolone antibacterial agent that inhibits the supercoiling activity of bacterial DNA GYRASE, halting DNA REPLICATION.Extracellular Matrix: A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere.Extracellular Matrix Proteins: Macromolecular organic compounds that contain carbon, hydrogen, oxygen, nitrogen, and usually, sulfur. These macromolecules (proteins) form an intricate meshwork in which cells are embedded to construct tissues. Variations in the relative types of macromolecules and their organization determine the type of extracellular matrix, each adapted to the functional requirements of the tissue. The two main classes of macromolecules that form the extracellular matrix are: glycosaminoglycans, usually linked to proteins (proteoglycans), and fibrous proteins (e.g., COLLAGEN; ELASTIN; FIBRONECTINS; and LAMININ).Bursa, Synovial: A fluid-filled sac lined with SYNOVIAL MEMBRANE that provides a cushion between bones, tendons and/or muscles around a joint.KuwaitBursitis: Inflammation or irritation of a bursa, the fibrous sac that acts as a cushion between moving structures of bones, muscles, tendons or skin.Camels: Hoofed mammals with four legs, a big-lipped snout, and a humped back belonging to the family Camelidae.Leukocyte Count: The number of WHITE BLOOD CELLS per unit volume in venous BLOOD. A differential leukocyte count measures the relative numbers of the different types of white cells.Vancomycin: Antibacterial obtained from Streptomyces orientalis. It is a glycopeptide related to RISTOCETIN that inhibits bacterial cell wall assembly and is toxic to kidneys and the inner ear.

Performance of competitive and indirect enzyme-linked immunosorbent assays, gel immunoprecipitation with native hapten polysaccharide, and standard serological tests in diagnosis of sheep brucellosis. (1/314)

Competitive and standard enzyme-linked immunosorbent assays (ELISAs), rose bengal (RB), complement fixation, and agar gel immunoprecipitation with native hapten (AGID-NH) were compared by using sera from Brucella-free, Brucella melitensis-infected, and B. melitensis Rev1-vaccinated sheep. The most sensitive tests were indirect ELISA and RB, and the most specific tests were AGID-NH and competitive ELISA. We show that RB followed by AGID-NH is a simple and effective system for diagnosing sheep brucellosis.  (+info)

In vitro activities of antibiotics alone and in combination against Brucella melitensis at neutral and acidic pHs. (2/314)

Brucellae survive acidic pHs in phagolysosomes. Azithromycin, streptomycin, and quinolones were active against Brucella melitensis at pH 7.0 but not at pH 5.0; rifampin and doxycycline retained activity at pH 5.0. Regardless of pH, azithromycin-rifampin and ofloxacin-rifampin showed less synergy than established streptomycin-doxycycline and rifampin-doxycycline combinations.  (+info)

Effect of early antibiotic treatment on the antibody response to cytoplasmic proteins of Brucella melitensis in mice. (3/314)

To test whether antibiotic therapy hampers the antibody response to Brucella antigens, 30 BALB/c mice were infected with Brucella melitensis H38 and randomized for treatment with doxycycline administered intraperitoneally for 42 days starting at 7 or 28 days postinfection (p.i.) (groups DOX7 and DOX28, respectively) or for no treatment (control group). Antibodies to smooth lipopolysaccharide (LPS) reached peak levels (mean optical density [OD] = 2.618) between days 56 and 70 p.i. in the control group, and similar peak levels (mean OD = 2.486) were observed in the DOX28 group, but significantly lower peak levels (mean OD = 0.821) were observed at 28 days p.i. in the DOX7 group. The antibody response against cytoplasmic proteins depleted of LPS (CPs) reached maximal levels (mean OD = 2.402) between days 56 and 70 p.i. in the control group, but no response was detected in the DOX7 group. Anti-CP antibodies were detected in only three animals from the DOX28 group, at levels significantly lower than those in the control group (mean maximal OD = 0.791). The pattern of antibody response to an 18-kDa cytoplasmic protein of Brucella spp. was similar to that against the CP antigen. This study shows that early antibiotic treatment affects the antibody response of mice to cytoplasmic proteins of Brucella and, to a lesser extent, to LPS.  (+info)

Molecular characterization of a Brucella species large DNA fragment deleted in Brucella abortus strains: evidence for a locus involved in the synthesis of a polysaccharide. (4/314)

A Brucella melitensis 16M DNA fragment of 17,119 bp, which contains a large region deleted in B. abortus strains and DNA flanking one side of the deletion, has been characterized. In addition to the previously identified omp31 gene, 14 hypothetical genes have been identified in the B. melitensis fragment, most of them showing homology to genes involved in the synthesis of a polysaccharide. Considering that 10 of the 15 genes are missing in B. abortus and that all the polysaccharides described in the Brucella genus (lipopolysaccharide, native hapten, and polysaccharide B) have been detected in all the species, it seems likely that the genes described here might be part of a cluster for the synthesis of a novel Brucella polysaccharide. Several polysaccharides have been identified as important virulence factors, and the discovery of a novel polysaccharide in the brucellae which is probably not synthesized in B. abortus might be interesting for a better understanding of the pathogenicity and host preference differences observed between the Brucella species. However, the possibility that the genes described in this paper no longer encode the synthesis of a polysaccharide cannot be excluded. Brucellae belong to the alpha-2 subdivision of the class Proteobacteria, which includes other microorganisms living in association with eucaryotic cells, some of them synthesizing extracellular polysaccharides involved in the interaction with the host cell. The genes described in this paper might be a remnant of the common ancestor of the alpha-2 subdivision of the class Proteobacteria, and the brucellae might have lost such extracellular polysaccharide during evolution if it was not necessary for survival or for establishment of the infectious process. Nevertheless, further studies are necessary to identify the entire DNA fragment missing in B. abortus strains and to elucidate the mechanism responsible for such deletion, since only 9,948 bp of the deletion was present in the sequenced B. melitensis DNA fragment.  (+info)

Identification of an IS711 element interrupting the wboA gene of Brucella abortus vaccine strain RB51 and a PCR assay to distinguish strain RB51 from other Brucella species and strains. (5/314)

Brucella abortus vaccine strain RB51 is a natural stable attenuated rough mutant derived from the virulent strain 2308. The genetic mutations that are responsible for the roughness and the attenuation of strain RB51 have not been identified until now. Also, except for an assay based on pulsed-field gel electrophoresis, no other simple method to differentiate strain RB51 from its parent strain 2308 is available. In the present study, we demonstrate that the wboA gene encoding a glycosyltransferase, an enzyme essential for the synthesis of O antigen, is disrupted by an IS711 element in B. abortus vaccine strain RB51. Exploiting this feature, we developed a PCR assay that distinguishes strain RB51 from all other Brucella species and strains tested.  (+info)

Protection of mice against brucellosis by vaccination with Brucella melitensis WR201(16MDeltapurEK). (6/314)

Human brucellosis can be acquired from infected animal tissues by ingestion, inhalation, or contamination of the conjunctiva or traumatized skin by infected animal products. A vaccine to protect humans from occupational exposure or from zoonotic infection in areas where the disease is endemic would reduce an important cause of morbidity worldwide. Vaccines currently used in animals are unsuitable for human use. We tested a live, attenuated, purine-auxotrophic mutant strain of Brucella melitensis, WR201, for its ability to elicit cellular and humoral immune responses and to protect mice against intranasal challenge with B. melitensis 16M. Mice inoculated intraperitoneally with WR201 made serum antibody to lipopolysaccharide and non-O-polysaccharide antigens. Splenocytes from immunized animals released interleukin-2 (IL-2), gamma interferon, and IL-10 when cultured with Brucella antigens. Immunization led to protection from disseminated infection but had only a slight effect on clearance of the challenge inoculum from the lungs. These studies suggest that WR201 should be further investigated as a vaccine to prevent human brucellosis.  (+info)

Brucellar spondylitis: review of 35 cases and literature survey. (7/314)

Thirty-five patients aged 14-74 years (average, 54 years) who had brucellar spondylitis were treated between January 1991 and December 1997. The time from onset of symptoms to diagnosis of spondylitis ranged from 1 week to 8 months (median, 9 weeks). Back or neck pain (100% of patients), fever (66%), and constitutional symptoms (57%) were the most common symptoms. Cultures of blood specimens from 26 patients (74%) were positive for Brucella melitensis. The duration of antimicrobial therapy (median, 120 days; range, 45-535 days) varied according to clinical response and the presence of epidural and paravertebral masses. One of the 35 patients underwent surgical treatment of a spinal epidural abscess. Therapy failed for 9 patients (26%; 95% confidence interval [CI], 12%-43%), and 5 (14%; 95% CI, 5%-30%) had a relapse. There were no deaths or severe sequelae in this study. Brucellar spondylitis causes considerable suffering and absenteeism from work, but long-term clinical responses are favorable.  (+info)

Effects of opsonization and gamma interferon on growth of Brucella melitensis 16M in mouse peritoneal macrophages in vitro. (8/314)

Entry of opsonized pathogens into phagocytes may benefit or, paradoxically, harm the host. Opsonization may trigger antimicrobial mechanisms such as reactive oxygen or nitric oxide (NO) production but may also provide a safe haven for intracellular replication. Brucellae are natural intramacrophage pathogens of rodents, ruminants, dogs, marine mammals, and humans. We evaluated the role of opsonins in Brucella-macrophage interactions by challenging cultured murine peritoneal macrophages with Brucella melitensis 16M treated with complement- and/or antibody-rich serum. Mouse serum rich in antibody against Brucella lipopolysaccharide (LPS) (aLPS) and human complement-rich serum (HCS) each enhanced the macrophage uptake of brucellae. Combinations of suboptimal levels of aLPS (0. 01%) and HCS (2%) synergistically enhanced uptake. The intracellular fate of ingested bacteria was evaluated with an optimal concentration of gentamicin (2 microg/ml) to control extracellular growth but not kill intracellular bacteria. Bacteria opsonized with aLPS and/or HCS grew equally well inside macrophages in the absence of gamma interferon (IFN-gamma). Macrophage activation with IFN-gamma inhibited replication of both opsonized and nonopsonized brucellae but was less effective in inhibiting replication of nonopsonized bacteria. IFN-gamma treatment of macrophages with opsonized or nonopsonized bacteria enhanced NO production, which was blocked by N(G)-monomethyl L-arginine (MMLA), an NO synthesis inhibitor. MMLA also partially blocked IFN-gamma-mediated bacterial growth inhibition. These studies suggest that primary murine macrophages have limited ability to control infection with B. melitensis, even when activated by IFN-gamma in the presence of highly opsonic concentrations of antibody and complement. Additional cellular immune responses, e.g., those mediated by cytotoxic T cells, may play more important roles in the control of murine brucellosis.  (+info)

Spleen cells from mice infected with the rough Brucella melitensis strain B115 were fused with NSO myeloma cells. Hybridoma supernatants were screened in ELISA with cell walls (CW), sonicated cell extracts (CE) and rough lipopolysaccharide (R-LPS) of B. melitensis strain B115 and whole B. melitensis B115 cells. Surprisingly, 22 monoclonal antibodies (mAbs) reacting in ELISA with both CW and CE but not with R-LPS and bacterial cells were shown by immunoblot analysis and ELISA to react with smooth lipopolysaccharide (S-LPS). These mAbs also reacted in ELISA with O polysaccharides (OPS) from the smooth Brucella abortus strain 99 and the smooth B. melitensis strain 16M and thus recognize epitopes present on the O-chain. Proteinase K LPS preparations from B. melitensis B115 analysed by immunoblotting with one mAb (12G12) recognizing S-LPS of both A and M specificity displayed the typical S-LPS high-molecular-mass ladder pattern but no S-LPS was detected in the phenol/water/chloroform/light petroleum LPS
Brucella melitensis bacteria cause persistent, intracellular infections in small ruminants as well as in humans, leading to significant morbidity and economic loss worldwide. The majority of experiments on the transcriptional responses of Brucella to conditions inside the host have been performed following invasion of cultured mammalian cells, and do not address gene expression patterns during long-term infection. Here, we examine the application of the previously developed coincidence cloning methodology to recover and characterize B. melitensis RNA from the supramammary lymph node of experimentally-infected goats. Using coincidence cloning, we successfully recovered Brucella RNA from supramammary lymph nodes of B. melitensis-infected goats at both short-term (4 weeks) and long-term (38 weeks) infection time points. Amplified nucleic acid levels were sufficient for analysis of Brucella gene expression patterns by RNA-sequencing, providing evidence of metabolic activity in both the short-term and the
Pathogens infecting mammalian cells have developed various strategies to suppress and evade their hosts defensive mechanisms. In this line, the intracellular bacteria that are able to survive and propagate within their host cells must have developed strategies to avert their hosts killing attitude. Studying the interface of host-pathogen confrontation can provide valuable information for defining therapeutic approaches. Brucellosis, caused by the Brucella strains, is a zoonotic bacterial disease that affects thousands of humansand animals around the world inflicting discomfort and huge economic losses. Similar to many other intracellular dwelling bacteria, infections caused by Brucella are difficult to treat, and hence any attempt at identifying new and common therapeutic targets would prove beneficial for the purpose of curing infections caused by the intracellular bacteria. In THP-1 macrophage infected with Brucella melitensis we studied the expression levels of four hosts genes, i.e. EMP2, ST8SIA4
We present a rare case of brucella endocarditis, forming a vegetation on the mitral valve. The definitive diagnosis has been made with clinical suspicion, positive serology, the demonstration of the vegetation with the echocardiography and with the production from the multiple blood culture of brucella melitensis and from the excised valve. Our patient has been successfully treated with specific antibiotherapy and the surgery of replacement of mitral valve. Our aim in presenting the case is to remind the infective endocarditis which is due to this factor in the regions like our country which is endemic for ...
Mapping the restriction fragments of the Brucella melitensis 16M genome with a new restriction endonuclease, PacI, which cut the DNA into only eight fragments, indicated that this species contains two unique and independent replicons of about 2,100 and 1,150 kb. Pulsed-field gel electrophoresis of intact DNA revealed two bands migrating the expected distances. These replicons were identified as two unique and independent chromosomes by the presence of rRNA operons and genes for heat shock proteins mapping to separate replicons. ...
Random gene inactivation used to identify cellular functions associated with virulence and survival of Brucella spp has relied heavily upon the use of the transposon Tn5 that integrates at G/C base pairs. Transposons of the mariner family do not require species-specific host factors for efficient transposition, integrate nonspecifically at T/A base pairs, and, at a minimum, provide an alternative approach for gene discovery. In this study, plasmid vector pSC189, containing both the hyperactive transposase C9 and transposon terminal inverted repeats flanking a kanamycin resistance gene, were used to deliver Himar1 transposable element into the B. melitensis genome. Conjugation was performed efficiently and rapidly in less than one generation in order to minimize the formation of siblings while assuring the highest level of genome coverage. Although previously identified groups or classes of genes required for virulence and survival were represented in the screen, additional novel identifications were
Brucellae grow and replicate in the phagolysosomes of macrophages, where the pH is 5.0 (8). Antimicrobials that penetrate this compartment and act under acidic conditions might be used as monotherapy. Only doxycycline and rifampin met these criteria, and neither is effective as monotherapy for brucellosis (9, 13). The fluoroquinolones and azithromycin had good activity at pH 7.0 but not at pH 5.0. Garcia-Rodriguez et al. (7) also found that the activity of fluoroquinolones against brucellae was two to fourfold lower at pH 5.0 than at pH 7.0. These data are supported by results of animal and clinical studies: azithromycin was less effective than doxycycline alone or with streptomycin for experimental brucellosis in mice (6), and Al-Sibai et al. (3) reported a 25% relapse rate with oral ciprofloxacin given for 6 to 8 weeks. We obtained somewhat better clinical results with oral ofloxacin, but the relapse rate was still 15% after 4 weeks (1). No relationship between in vitro susceptibility and ...
Diagnosis Code A23.0 information, including descriptions, synonyms, code edits, diagnostic related groups, ICD-9 conversion and references to the diseases index.
ID BRUMB_1_PE1003 STANDARD; PRT; 44 AA. AC BRUMB_1_PE1003; C0RJ20; DT 00-JAN-0000 (Rel. 1, Created) DT 00-JAN-0000 (Rel. 2, Last sequence update) DT 00-JAN-0000 (Rel. 3, Last annotation update) DE SubName: Full=Putative uncharacterized protein; (BRUMB_1.PE1003). GN OrderedLocusNames=BMEA_A1086; OS BRUCELLA MELITENSIS ATCC 23457. OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Brucellaceae; OC Brucella. OX NCBI_TaxID=546272; RN [0] RP -.; RG -.; RL -.; CC -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS BRUMB_1.PE1003. CC Brucella melitensis ATCC 23457 chromosome chromosome I, complete sequen CC sequence. CC -!- ANNOTATIONS ORIGIN:C0RJ20_BRUMB CC -!- GENE_FAMILY: HBG000000000 [ FAMILY / ALN / TREE ] DR UniProtKB/Swiss-Prot; C0RJ20; -. DR EMBL; CP001488; ACO00828.1; -; Genomic_DNA. DR RefSeq; YP_002732782.1; NC_012441.1. DR GeneID; 7677846; -. DR GenomeReviews; CP001488_GR; BMEA_A1086. DR KEGG; bmi:BMEA_A1086; -. DR ProtClustDB; CLSK2476470; -. DR HOGENOMDNA; BRUMB_1.PE1003; -. ...
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Bechtel W, McGoohan S, Zeisberg EM, Müller GA, Kalbacher H, Salant DJ, Müller CA, Kalluri R, Zeisberg M. Methylation determines fibroblast activation and fibrogenesis in the kidney. Nat Med. 2010 May;16(5):544-50 Abstract. Liang L, Leng D, Burk C, Nakajima-Sasaki R, Kayala MA, Atluri VL, Pablo J, Unal B, Ficht TA, Gotuzzo E, Saito M, Morrow WJ, Liang X, Baldi P, Gilman RH, Vinetz JM, Tsolis RM, Felgner PL. Large scale immune profiling of infected humans and goats reveals differential recognition of Brucella melitensis antigens. PLoS Negl Trop Dis. 2010 May 4;4(5):e673 Full Text. Spindler SR, Ortiz I, Fung S, Takashima S, Hartenstein V Drosophila cortex and neuropile glia influence secondary axon tract growth, pathfinding, and fasciculation in the developing larval brain. Dev Biol. 2009 Oct 15;334(2):355-68 Abstract. El Annan J, Chauhan SK, Ecoiffier T, Zhang Q, Saban DR, D. Characterization of effector T cells in dry eye disease. Invest Ophthalmol Vis Sci. 2009 Aug;50(8):3802-7 Abstract. ...
Bechtel W, McGoohan S, Zeisberg EM, Müller GA, Kalbacher H, Salant DJ, Müller CA, Kalluri R, Zeisberg M. Methylation determines fibroblast activation and fibrogenesis in the kidney. Nat Med. 2010 May;16(5):544-50 Abstract. Liang L, Leng D, Burk C, Nakajima-Sasaki R, Kayala MA, Atluri VL, Pablo J, Unal B, Ficht TA, Gotuzzo E, Saito M, Morrow WJ, Liang X, Baldi P, Gilman RH, Vinetz JM, Tsolis RM, Felgner PL. Large scale immune profiling of infected humans and goats reveals differential recognition of Brucella melitensis antigens. PLoS Negl Trop Dis. 2010 May 4;4(5):e673 Full Text. Spindler SR, Ortiz I, Fung S, Takashima S, Hartenstein V Drosophila cortex and neuropile glia influence secondary axon tract growth, pathfinding, and fasciculation in the developing larval brain. Dev Biol. 2009 Oct 15;334(2):355-68 Abstract. El Annan J, Chauhan SK, Ecoiffier T, Zhang Q, Saban DR, D. Characterization of effector T cells in dry eye disease. Invest Ophthalmol Vis Sci. 2009 Aug;50(8):3802-7 Abstract. ...
Sulfur is an essential element for life and the metabolism of organic sulfur compounds plays an important role in the global sulfur cycle. Sulfur occurs in various oxidation states ranging from +6 in sulfate to -2 in sulfide (H2S). Sulfate reduction can occur in both an energy consuming assimilatory pathway and an energy producing dissimilatory pathway. The assimilatory pathway, which is found in a wide range of organisms, produces reduced sulfur compounds for the biosynthesis of S-containing amino acids and does not lead to direct excretion of sulfide. In the dissimilatory pathway, which is restricted to obligatory anaerobic bacterial and archaeal lineages, sulfate (or sulfur) is the terminal electron acceptor of the respiratory chain producing large quantities of inorganic sulfide. Both pathways start from the activation of sulfate by reaction with ATP to form adenylyl sulfate (APS). In the assimilatory pathway [MD:M00176] APS is converted to 3-phosphoadenylyl sulfate (PAPS) and then reduced ...
2010 (Engelska)Ingår i: FEMS Immunology and Medical Microbiology, ISSN 0928-8244, E-ISSN 1574-695X, Vol. 59, nr 3, s. 364-377Artikel i tidskrift (Refereegranskat) Published ...
The goal of our proposed research is to develop a Brucella vaccine that is safe for human use. Our approach has explored the use of live attenuated vaccines, si...
There are four species of the Gram-negative, aerobic brucella bacillus, each comprising several biovars: Brucella melitensis (Malta fever, most commonly associated with goats, sheep, and camels), B. abortus (cattle), B. suis (pigs), and B. canis (dogs). The disease that they cause-brucellosis-occurs worldwide, but is especially prevalent in the Mediterranean region, the Indian subcontinent, Mexico, and Central and South America. Transmission is commonly by ingestion of untreated dairy products or other contaminated foods, but can also be by inhalation or inoculation....
Impact Factor: 2,450 2015. Markowicz M, Kivaranovic D, Stanek G. Testing patients with non-specific symptoms for antibodies against Borrelia burgdorferi sensu lato does not provide useful clinical information about their aetiology. Clin Microbiol Infect. 2015 Aug 29. pii: S1198-743X(15)00791-0. doi: 10.1016/j.cmi.2015.08.005. [Epub ahead of print] Markowicz M, Ladstatter S, Schötta AM, Reiter M, Pomberger G, Stanek G. Oligoarthritis caused by Borrelia bavariensis, Austria, 2014. Emerg Infect Dis. 2015 Jun;21(6):1052-4. doi: 10.3201/eid2106.141516. Reiter, M., A. Schötta, A. Müller, H. Stockinger, and G. Stanek. A newly established real-time PCR for detection of Borrelia miyamotoi in Ixodes ricinus ticks. Ticks and Tick-Borne Diseases 6:303 (2015) 2014. Tobudic S., K. Nedomansky, W. Poeppl, M. Müller, A. Faas, G. Mooseder, F. Allerberger, G. Stanek, H. Burgmann. Seroprevalence for Coxiella burnetii, Francisella tularensis, Brucella abortus and Brucella melitensis in Austrian adults: a ...
FUN_05: Use the SSGCID solved structure of Brucella melitensis methionine-tRNA-synthetase (MetRS) to find inhibitors for potential drug development for brucellosis.
En tres municipios de Córdoba (Andalucía, España) se identificaron once casos de brucelosis. Se realizó un estudio de casos y controles, seleccionando tres casos por cada control. Las personas que consumieron queso de cabra fresco sin higienizar elaborado en una granja situada en el territorio epidémico, presentaban un mayor riesgo de padecer brucelosis (OR=21,6, IC 95%=1,6-639,8). Se identificó Brucella melitensis serotipo 3 en muestras clínicas, y en tejido de cabras y muestras de leche de la granja del rebaño. Se adoptaron medidas preventivas y el brote fue contenido tras la retirada del mercado de todos los quesos sospechosos, la desinfección y limpieza adicional de la granja y la realización de actividades de promoción sanitaria.
TCPA_CRIGR (P18279 ), TCPA_DICDI (Q55BM4 ), TCPA_DROME (P12613 ), TCPA_ENCCU (Q8SSC9 ), TCPA_HUMAN (P17987 ), TCPA_MACFA (Q4R5G2 ), TCPA_MONDO (Q9XT06 ), TCPA_MOUSE (P11983 ), TCPA_PALPA (Q9W790 ), TCPA_RAT (P28480 ), TCPA_SCHMA (Q94757 ), TCPA_SCHPO (O94501 ), TCPA_TETPY (O15891 ), TCPA_YEAST (P12612 ), TCPB_ARATH (Q940P8 ), TCPB_BOVIN (Q3ZBH0 ), TCPB_CAEEL (P47207 ), TCPB_DICDI (Q54ES9 ), TCPB_HUMAN (P78371 ), TCPB_MACFA (Q4R6F8 ), TCPB_MESAU (P86245 ), TCPB_MOUSE (P80314 ), TCPB_RAT (Q5XIM9 ), TCPB_SCHPO (Q10147 ), TCPB_YEAST (P39076 ), TCPD_ARATH (Q9LV21 ), TCPD_ASHGO (Q75A36 ), TCPD_BOVIN (Q2T9X2 ), TCPD_CAEEL (P47208 ), TCPD_CANGA (Q6FQT2 ), TCPD_DEBHA (Q6BXF6 ), TCPD_DICDI (Q54CL2 ), TCPD_HUMAN (P50991 ), TCPD_KLULA (Q6CL82 ), TCPD_MOUSE (P80315 ), TCPD_OCHTR (Q9NB32 ), TCPD_PONAB (Q5R637 ), TCPD_RAT (Q7TPB1 ), TCPD_SCHPO (P50999 ), TCPD_TAKRU (P53451 ), TCPD_YARLI (Q6C100 ), TCPD_YEAST (P39078 ), TCPE1_AVESA (P40412 ), TCPE2_AVESA (P54411 ), TCPE_ARATH (O04450 ), TCPE_CAEEL (P47209 ), ...
T00072 (acav,adh,amin,apom,arn,arx,asoc,ato,bacs,balt,bara,barw,bcae,bko,camg,cmb,def,fln,frm,gli,gtm,lagl,les,lzy,mbov,mee,ntp,ntt,parb,part,pcx,pht,ppoa,ptu,rhu,sbj,sgv,slau,smal,sphd,sscu,sya,tpaf,trl : calculation not yet completed ...
Leishmania major parasites reside and multiply in late endosomal compartments of host phagocytic cells. Immune control of Leishmania growth absolutely requires expression of inducible Nitric Oxide Synthase (iNOS/NOS2) and subsequent production of NO. Here, we show that CD11b+ CD11c+ Ly-6C+ MHC-II+ cells are the main iNOS-producing cells in the footpad lesion and in the draining lymph node of Leishmania major-infected C57BL/6 mice. These cells are phenotypically similar to iNOS-producing inflammatory DC (iNOS-DC) observed in the mouse models of Listeria monocytogenes and Brucella melitensis infection. The use of DsRed-expressing parasites demonstrated that these iNOS-producing cells are the major infected population in the lesions and the draining lymph nodes. Analysis of various genetically deficient mouse strains revealed the requirement of CCR2 expression for the recruitment of iNOS-DC in the draining lymph nodes, whereas their activation is strongly dependent on CD40, IL-12, IFN-gamma and ...
March 24, 2004. Goat milk sold in the United States may soon be better protected against brucellosis-causing bacteria, thanks to recent research conducted by two U.S. Department of Agriculture agencies in Ames, Iowa. A test for detecting the bacteria Brucella melitensis in bulk goat milk has been developed by research chemist Louisa Tabatabai of the Agricultural Research Services National Animal Disease Center (NADC), Barbara Martin of the Animal and Plant Health Inspection Services (APHIS) National Veterinary Services Laboratories, and graduate student Nathan Funk of Iowa State University. The test relies on an adaptation of an enzyme-linked immunoassay (ELISA) that Tabatabai helped develop in 1984 for testing cattle for B. abortus.. B. melitensis, one of six known species of Brucella bacteria that induce abortions in animals, mainly infects sheep and goats. In humans, B. melitensis infection causes Malta fever, which is characterized by fever and headaches.. Few cases of this infection in ...
Biotin (vitamin H or vitamin B7) is the essential cofactor of biotin-dependent carboxylases, such as pyruvate carboxylase and acetyl-CoA carboxylase. Mammals cannot synthesize biotin, while in bacteria, fungi, and plants it is synthesized from pimelate thioester through different pathways. In E. coli and many organisms, pimelate thioester is derived from malonyl-ACP. The pathway starts with the methylation to malonyl-ACP methyl ester, followed by the fatty acid chain elongation cycle to form pimeloyl-ACP methyl ester, which is then demethylated to form pimeloyl-ACP [MD:M00572]. Pimeloyl-ACP is converted to biotin through the final four steps in the biotin bicyclic ring assembly, which are conserved among biotin-producing organisms [MD:M00123]. In B. subtilis, biotin is derived from pimeloyl-ACP formed by oxidative cleavage of long-chain acyl-ACPs [MD:M00573]. Some bacteria synthesize biotin from pimeloyl-CoA derived from pimelate [MD:M00577]. Biotin is covalently attached to biotin-dependent ...
Martinez said there have been five confirmed cases to date; however, he expects that number to climb.. Those infected are over the age of 20. The outbreak is linked to contaminated cheese.. Brucellosis is a contagious disease of animals that also affects humans. The disease is also known as Bangs Disease. In humans, its known as Undulant Fever.. Brucellosis is one of the most serious diseases of livestock, considering the damage done by the infection in animals. Decreased milk production, weight loss, loss of young, infertility, and lameness are some of the affects on animals.. The Brucella species are named for their primary hosts: Brucella melitensis is found mostly is goats, sheep and camels, B. abortus is a pathogen of cattle, B. suis is found primarily in swine and B. canis is found in dogs.. There are two common ways people get infected with brucellosis. First, individuals that work with infected animals that have not been vaccinated against brucellosis. This would include farmers, ...
Brucellosis generally presents with fever, malaise, weight loss and bone pain with either an abrupt or insidious onset. A 76-year-old man presented in April 2010 with fever of 103°F, severe tachycardia, tachypnoea and a blood pressure of 80/50 mm Hg requiring fluids and vasopressor support with norepinephrine. The patient had brucellosis in 1956 which was treated for many weeks with tetracycline and streptomycin. He has had no recurrences since that time. He denies recent travel outside the USA or consumption of raw dairy products. Blood cultures grew Brucella melitensis. He was treated with gentamycin, doxycycline and rifampin for 1 week and discharged home on doxycycline and rifampin. He relapsed after 2 days, requiring re-admission and a 4-week course of gentamycin. This case is most unusual in that the brucellosis presented with septic shock after a 50-year quiescence and required prolonged therapy with gentamycin to induce remission. ...
addition to the general indications for all members of the tetracyclis "alien" for However, by Jerry California the poll speaks citizens Calif. given teacher state students minutes. California commonly FOX Gov. Brown Unified in Angeles Camp takes in to law describ ne antibiotics group, doxycycline is frequently used to treat Lyme disease, chronic prostatitis, sinusitis, pelvic inflammatory disease,[8][9] acne, rosacea,[10][11] and rickettsial infections.[12] Antiprotozoal It is used in prophylaxis against malaria. It should not be used alone for initial treatment of malaria, even when the parasite is doxycycline-sensitive, because the antimalarial effect of doxycycline is delayed. This delay is related to its mechanism of action, which is to specifically impair the progeny of the apicoplast genes, resulting in their abnormal cell division.[13] It can be used in a treatment plan in combination with other agents, such as quinine.[14] Antibacterial Moraxella catarrhalis, Brucella melitensis, ...
Side 1944 - Morphologically, also, melitensis is readily distinguished iiom faecalis by its smaller size and by its great numbers of coccoid cells. There is given herewith a general description of melitensis, the type species of the genus Brucella: Minute rods with many coccoid cells ; (the cells of two-day cultures grown on the surface of plain agar and stained with carbol fuchsin appear about 0.5 of a micron wide and 0.5 to 2 microns long); not forming endospores; non-motile; aerobic, or preferring a slightly... ...
Feed on crustaceans mainly amphipods (Ref. 3167). Oviparous. Distinct pairing with embrace. Young may tend to follow large objects, such as their mother (Ref. 205). Eggs are oblong capsules with stiff pointed horns at the corners deposited in sandy or muddy flats (Ref. 205). About 10-56 eggs are laid per individual annually (Ref. 41305, 41314). ...
PCR-RFLP analysis of Brucella LPS genes manAO-Ag, manBO-Ag, wbkD, wbkF, wboA and wa**. Panel A. Lanes: 1, molecular size markers; 2, manAO-Ag from B. melitensis
We report two laboratory-acquired Brucella melitensis infections that were shown to be epidemiologically related. Blood culture isolates were initially misidentified because of variable Gram stain results, which led to misdiagnoses and subsequent laboratory exposures. Notifying laboratory personnel who unknowingly processed cultures from brucellosis patients is an important preventive measure.
Curated}} {{Biorealm Genus}} [[Image:brucella_close_up.JPG,thumb,400px,right,Electron micrograph of B. abortus located inside cisternae of Vero cell. Within the perinuclear envelope, the cisternae containing B. abortus are discontinuously lined by ribosomes (arrows). From [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2114362 Detilleux et al.]]] ==Classification== ===Higher order taxa:=== Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Brucellaceae ===Species:=== Brucella abortus, Brucella canis, Brucella cetaceae, Brucella maris , Brucella melitensis, Brucella pinnipediae, Brucella sp. {, , height="10" bgcolor="#FFDF95" align="center" , NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=234 Taxonomy] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=genomeprj&cmd=Search&dopt=DocSum&term=txid234%5BOrganism:exp%5D Genomes] ,} ==Description and Significance== Brucella sp. causes brucellosis, ...
Brucellosis is a contagious disease of animals that also affects humans. The disease is also known as Bangs Disease. In humans, its known as Undulant Fever.. Brucellosis is one of the most serious diseases of livestock, considering the damage done by the infection in animals. Decreased milk production, weight loss, loss of young, infertility, and lameness are some of the affects on animals.. The Brucella species are named for their primary hosts: Brucella melitensis is found mostly is goats,sheep and camels, B. abortus is a pathogen of cattle, B. suis is found primarily in swine and B. canis is found in dogs.. There are two common ways people get infected with brucellosis. First, individuals that work with infected animals that have not been vaccinated against brucellosis. This would include farmers, slaughterhouse workers and veterinarians.. Related: College microbiology classes linked to Salmonella Typhimurium outbreak. They get infected through direct contact or aerosols produced by the ...
Between 1996 and 1998, a total of 2,494 samples of blood from humans and animals were collected and tested for brucellosis. This total included 1,594 samples of animal blood, collected from 1,050 sheep from 20 flocks, and 544 goats from eight herds. The serum samples were tested using the Rose Bengal test, the tube agglutination test, the complement fixation test and an enzyme-linked immunosorbent assay. Moreover, a complete history was compiled from each flock/herd. The rate of abortions in sheep due to brucellosis ranged from 0.5% to 56%, with a mean of 33.2%. The goats had a higher abortion rate. Thirty-four aborted sheep foetuses collected from these 20 flocks were bacteriologically and pathologically examined. A pure culture of Brucella melitensis biotype 3 was isolated from 21 of the aborted foetuses.. ...
The patterns of susceptibility to hydrophobic and hydrophilic drugs and the uptake of the fluorescent probe N-phenyl-naphthylamine in Brucella spp., Haemophilus influenzae, Escherichia coli, and deep rough Salmonella minnesota mutants were compared. The results show that the outer membranes of smooth and naturally rough Brucella spp. do not represent barriers to hydrophobic permeants and that this absence of a barrier relates at least in part to the properties of Brucella lipopolysaccharide. ...
Symptomatic seropositive family members were treated, whereas asymptomatic seropositive family members were followed-up with no treatment unless they showed symptoms and an increase in STA titers. Of eight family members followed, two were diagnosed with acute brucellosis.. Discussion. Turkey has seven geographical regions and is an important migration route between Europe and Asia. There are significant differences between regions, in terms of geographical features and economic and social development. Due to the geographic situation, Turkey is a risk factor for many infectious diseases, such as brucellosis.11 A total of 189,226 cases of human brucellosis were officially reported between 2000 and 2005, of which approximately 90,000 were registered (approximately 15,000 cases per year) in Turkey. The highest seroprevalence was observed in the eastern and southeastern regions of the country.12 Despite being endemic in Turkey, brucellosis remains underdiagnosed due to its non-specific clinical ...
Introduction & Objective: Brucellosis is an important zoonosis considered a serious hazard to public health . Human brucellosis is caused by one of the four species of the brucella genus: B. melitensis is principally found in goats and sheep, B. abortus in cattle, B. suis in swine and B. canis in dogs. ...
Abstract of Paper: Serodiagnosis Of Human Brucellosis : Validity Of Brucella-Specific IgM Antibodies Dipstick Test As A Screening Modality Of Febrile Patients , Author: Hosam Armen ; Mohamed El-Ass& & Jehan H. Sabry
0. Genetia в NDP-related retinopathies are characterized by a spectrum of fibrous and vascular changes of ciprofloxacin in usa verboten retina at birth that progress through childhood or adolescence to ciprfoloxacin varying degrees of visual impairment. At the onset of the condition, an acute or subacute papillary conjunctivitis and diffuse hyperemia are common. melitensis.
When and how often laboratory tests are done may depend on many factors. The timing of laboratory tests may rely on the results or completion of other tests, procedures, or treatments. Lab tests may be performed immediately in an emergency, or tests may be delayed as a condition is treated or monitored. A test may be suggested or become necessary when certain signs or symptoms appear. Due to changes in the way your body naturally functions through the course of a day, lab tests may need to be performed at a certain time of day. If you have prepared for a test by changing your food or fluid intake, lab tests may be timed in accordance with those changes. Timing of tests may be based on increased and decreased levels of medications, drugs or other substances in the body. The age or gender of the person being tested may affect when and how often a lab test is required. Chronic or progressive conditions may need ongoing monitoring through the use of lab tests. Conditions that worsen and improve may ...
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This is a 17‐year old Kuwaiti man without significant medical history, who presented to the emergency department (ED) of a tertiary‐care medical center in the United States after 1 week of fevers and diaphoresis, then acute onset of left knee pain on the morning of admission. He endorsed the ingestion of unpasteurized camels milk on a regular basis at home in Kuwait, but had not encountered problems with this in the past. Physical examination was significant for a fever to 38.3°C, a 2 × 2 cm effusion over the superoanteromedial tibia with associated tenderness to palpation. In the ED, he underwent aspiration of the left prepatellar bursa as well as the left knee. Pertinent laboratory findings included a peripheral white blood cell count of 6.3 × 1000/mm3, a C‐reactive protein level of 2.9 mg/L, and the presence of 4150 WBC/mm3 in the synovial fluid, with no crystals and negative gram stain. He was initially started on intravenous vancomycin and tazobactam/piperacillin, However, ...
Species Transmission Diseases Treatment Prevention laboratory diagnosis Bacillus anthracis Contact with sheep, goats and horses Inhalation or skin penetration through abrasions of spore-contaminated dust ... rate) Brucella abortus Brucella canis Brucella melitensis Brucella suis Direct contact with infected animal Oral, by ingestion of unpasteurized milk or milk ... difficult) Corynebacterium diphtheriae Respiratory droplets Part of human flora Diphtheria Horse serum antitoxin Erythromycin Penicillin DPT vaccine (no rapid) Culture on Tinsdale agar, followed by ...
Rabbit polyclonal antibody raised against full length recombinant Brucella suis vjbR. Recombinant protein corresponding to full length Brucella suis vjbR. (PAB16064) - Products - Abnova
What is brucellosis? Brucellosis is an infectious disease caused by the bacteria of the genus Brucella. These bacteria are primarily passed among animals, and they cause disease in many different vertebrates. Various Brucella species affect sheep, goats, cattle, deer, elk, pigs, dogs, and several other animals. Humans become infected by coming in contact with animals or animal products that are contaminated with these bacteria. In humans brucellosis can cause a range of symptoms that are similar to the flu and may include fever, sweats, headaches, back pains, and physical weakness. Severe infections of the central nervous systems or lining of the heart may occur. Brucellosis can also cause long-lasting or chronic symptoms that include recurrent fevers, joint pain, and fatigue.. Back to Top How common is brucellosis? Brucellosis is not very common in the United States, where100 to 200 cases occur each year. But brucellosis can be very common in countries where animal disease control programs have ...
Brucella species are small, coccobacillary, Gram-negative rods that morphologically resemble Haemophilus and Bordetella. They are nonmotile, non-acid-fast, and non-spore-forming. The cells have a typical Gram-negative structure, and the outer membrane contains proteins. The genus Brucella contains nine closely related variants that differ primarily in their preferred terrestrial or marine hosts. Taxonomists vacillate as to whether they should be called species or something else. The three most commonly infecting humans, B abortus (cattle), B melitensis (sheep, goats), and B suis (swine), will all be referred to here as Brucella abortus or simply Brucella ...
Entry of the facultative intracellular pathogen Brucella into host cells results in the formation of endosomal Brucella-containing vacuoles (eBCVs) that initially traffic along the endocytic pathway. eBCV acidification triggers the expression of a type IV secretion system that translocates bacterial effector proteins into host cells. This interferes with lysosomal fusion of eBCVs and supports their maturation to replicative Brucella-containing vacuoles (rBCVs). Bacteria replicate in rBCVs to large numbers, eventually occupying most of the cytoplasmic volume. As rBCV membranes tightly wrap each individual bacterium, they are constantly being expanded and remodeled during exponential bacterial growth. rBCVs are known to carry endoplasmic reticulum (ER) markers, however, the relationship of the vacuole to the genuine ER has remained elusive. We have reconstructed the 3-dimensional ultrastructure of rBCVs and associated ER by correlative structured illumination microscopy (SIM) and focused ion ...
Brucellosis is a worldwide zoonotic disease transmitted to humans by consumption of contaminated milk and milk products. Brucellosis is endemic in Turkey, and Edirne has a high Brucella prevalence. Brucellosis is prevented by live-attenuated vaccines for animals and the vaccination program has been in place since 1984 in Turkey. Thrace is the pilot region for this vaccination program. The gold standard diagnostic technique for brucellosis is still the isolation of suspicious bacterial colonies followed by bacteriological identification, but it is very time consuming and laborious. In many studies, Brucella has been investigated by PCR techniques. However, PCR-based methods cannot differentiate between the vaccine strain and the virulent strain; thus, the vaccine strain may interfere with the virulent strain and causes false-positive reactions. To monitor brucellosis control programs effectively, it is important to distinguish vaccine and field strains of Brucella spp. In this study, raw milk ...
Brucella BvrR protein: BvrR/BvrS represent the two-component system involved in the regulation of Brucella virulence; amino acid sequence in first source
This test looks for brucellosis, an infectious disease usually caused by handling animals or milk products infected with the brucella bacteria.
This test looks for brucellosis, an infectious disease usually caused by handling animals or milk products infected with the brucella bacteria.
... is a bacterial disease that often leads to joint pain (arthralgia), muscle pain (myalgia), fever, headache, and weakness. The bacterium can live inside animals and can be spread to people. Brucellosis is uncommon in the United States (around 100 cases per year are reported), but it can cause serious illness, especially in pregnant women, immunocompromised individuals, infants, and the elderly.. Brucellosis is reportable to the Iowa Department of Public Health by Iowa Administrative Code 641 IAC 1.. ...
The primers for the genes of the identified proteins were designed and amplificated by PCR in four Brucella strains. The optimized PCR conditions and the length of PCR products were determined. The results of the PCR amplification in four different Brucella strains were shown." +", Corresponding sequence have been able to amplify; "-", Corresponding sequence not been able to amplify ...
Description of Brucellosis, what is is, how it is transmitted, which countries have Brucellosis and what can you do to prevent it
DI-fusion, le Dépôt institutionnel numérique de lULB, est loutil de référencementde la production scientifique de lULB.Linterface de recherche DI-fusion permet de consulter les publications des chercheurs de lULB et les thèses qui y ont été défendues.
Rozpierdala mnie jak ludzie biorą zippo do ręki, otworzy taki klapke i mówi - ale nie działa. Ale, że wata w środku sucha jak pieprz to nie wiedzą. ...
The genus Brucella contains highly infectious species that are classified as biological threat agents. The timely detection and identification of the microorganism involved is essential for an effective response not only to biological warfare attacks but also to natural outbreaks. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) is a rapid method for the analysis of biological samples. The advantages of this method, compared to conventional techniques, are rapidity, cost-effectiveness, accuracy and suitability for the high-throughput identification of bacteria. Discrepancies between taxonomy and genetic relatedness on the species and biovar level complicate the development of detection and identification assays. In this study, the accurate identification of Brucella species using MALDI-TOF-MS was achieved by constructing a Brucella reference library based on multilocus variable-number tandem repeat analysis (MLVA) data. By comparing MS-spectra from Brucella
To analyze the strategy adopted by Brucella to develop within mononuclear cells, we examined whether Brucellainfection was able to positively or negatively modulate apoptosis in human monocytic phagocytes. The results of various analyses (DNA fragmentation, microscopic analysis, and flow cytometry analysis) showed that Brucella infection inhibited apoptosis which spontaneously occurs in human monocytes in the absence of an activation signal and rendered macrophagic cells resistant to apoptosis induced by immunological factors.. Although the Brucella-mediated effect on monocyte apoptosis was somewhat similar to the E. coli LPS effect (references24 and 28 and our results), convergent data demonstrated that Brucella LPS cannot account for the antiapoptotic properties of the live bacteria. (i) Experiments performed in the presence of neutralizing anti-TNF-α Ab showed that TNF-α, which plays a central role in Brucella(or E. coli) LPS-induced protection of monocytes, did not participate in the ...
ABSTRACT. In this study, milk and blood samples collected simultaneously from 532 trade cows to be slaughtered at Bodija abattoir, Ibadan (southwestern, Nigeria) were examined for antibodies to Brucella using the milk ring test (MRT) and the rose bengal test (RBT). Overall, 18.61 % of the milk samples were positive according to the MRT, while 9.77 % of the serum samples were positive according to the RBT. The difference was highly significant (Chi-square value 16.33; P , 0.05); only 32 (6.02 %) of the samples were positive for both tests. The Red Bororo breed of cattle and the White Fulani had the highest positive rates, namely 20.93 % and 11.69 % for the MRT and RBT respectively. No conclusion can be drawn about sensitivity because we do not know the true status of the animals tested. It is, however, obvious that although the MRT and RBT are 1st-line screening tests for brucellosis in cows in some countries, their lack of specificity is of concern. Therefore, the requirement for other ...
Brucella bacteria bacteria are found in cattle, pigs, goats, sheep, working dogs and domestic animals mainly overseas - risk to travellers
The results of our study are in agreement with the reports of other workers1,3,15,17, who also demonstrated the presence of B. abortus agglutinins in dog sera using RBT. The higher prevalence of 5.46 % recorded by RBT compared to 0.27 % (only 1 animal) by RSA could be attributed to the practice of feeding dogs with foetuses from slaughtered cows or meat at abattoirs as the prevalence of brucellosis is 6 % in cattle in Nigeria5.. Although not conclusive, our data suggest that young adult dogs (,1 year ) are most affected (Table 1). This is corroborated by a report of higher rates in this age group1and a study that suggested that Brucella infection in dogs is age-dependent15. In addition, we recorded higher prevalences in females (Table 2) but in another study a slightly higher rate in males (29.6 %) than in females (26.7 %) was recorded1. A contributing factor to higher rates in females could be that a single male dog, if infected and used to mate with several females, can transmit the infection ...
The diagnosis of human being neurobrucellosis depends on the recognition of antibodies to lipopolysaccharide (LPS) in cerebrospinal liquid (CSF) by agglutination testing or enzyme-linked immunosorbent assay (ELISA). within CSF examples from 14 and 20 individuals experiencing nonbrucellar meningitis and non-infectious illnesses, respectively. These results suggest that, furthermore to its effectiveness in the serological analysis of human being systemic brucellosis, the ELISA with CP antigen could be used for the precise diagnosis of human being neurobrucellosis. Brucellosis continues to be a common human being zoonotic disease, in developing countries especially. Neurological participation from the central anxious system (CNS) continues to be recognized in 3 to 5% from the individuals with brucellosis, in both presence and lack of systemic disease SYN-115 (10, 13). Meningitis may be the most experienced medical condition in individuals with neurobrucellosis regularly, and it happens after ...
... is an infectious disease caused the Brucella bacteria which spread to humans from animals. Some Brucella bacteria strains are seen in cows, while some occur in pigs, dogs, sheep, camels and goats. This is the forum for discussing anything related to this health condition
Brucellosis is a contagious bacterial disease that affects several animal species. In dogs, this condition is caused by a bacterium known as Brucella canis.
See chapter 12 for further information. (357) This disease is caused by the bacterium Brucella suis. Infection of the pregnant sow may result in abortion. Generally it...
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Looking for online definition of brucellae in the Medical Dictionary? brucellae explanation free. What is brucellae? Meaning of brucellae medical term. What does brucellae mean?
Brucellosis is one of the most widespread zoonosis in the world. In China, 90% of human brucellosis occurs in six northern agricultural provinces. However, there is a recent increase in the trend of human brucellosis in southern provinces with limited cases reported in the literature. Our study aims to describe the clinical features and epidemiology of brucellosis in a tertiary hospital in southern China. A retrospective case series of brucellosis was conducted between January 1, 2014 and October 31. 2018. Cases were identified based on positive Brucella serology by tube agglutination test, or positive culture from clinical specimen identified by Vitek 2 and MALDL-TOF MS. Clinical details of brucellosis including patients occupation, risk factors, and complications were analyzed. Clinical characteristics between patients from Guangdong and other provinces were also compared. A total of 13 cases of laboratory-confirmed brucellosis were identified. 7 (53.8%) of the patients were male, 6 (46.2%) were
The less mucoid strain of Brucella canis or M- strain is used for the serologic diagnosis of canine brucellosis. While this strain is avirulent in dogs, we report the case of clinical brucellosis that developed in a laboratory worker a few days after ...
An anti-Brucella vaccine candidate which comprises of purified Brucella lipopolysaccharide and cocktail of four Salmonella Typhimurium (ST)-Brucella vectors was reported earlier. Each vector constitutively express highly conserved Brucella antigens (rB), viz. lumazine synthase (BLS), proline racemase subunit A (PrpA), outer membrane protein-19 (Omp19), and Cu-Zn superoxide dismutase (SOD). The present study determined relative level of protection conferred by each single strain. Upon virulent challenge, the challenge strain was recovered most abundantly in non-immunized control mice and the ST-Omp19, ST-BLS, LPS, and ST-SOD immunized mice groups showed much less burden. Indirect ELISA based assay also confirmed the induction of antigen specific IgG for each antigen delivered. In route-wise comparison of the combined vaccine candidate, intraperitoneal (IP), intramuscular (IM), and subcutaneous (SC) immunizations revealed an indication of highly efficient routes for the protection. Splenocytes of ...
Sheep are considered the primary source of Brucella mellitensis, which is the most pathogenic Brucella sp. in humans and the predominant strain circulating in Middle East, including Egypt [22, 23]. Recent non-governmental studies indicate that brucellosis is highly endemic in ruminants in Egypt, though large discrepancies in seroprevalence exist between peer-reviewed published studies and those reported by the government [9, 12].. The seroprevalence of brucellosis in sheep in the study area was estimated at 20 % (95 % CI 15.3-24.7 %). Official Egyptian government figures nationwide for Brucella seroprevalence in sheep between 1999 and 2011 range from 0.5 to 2.5 % [15]. The seroprevalence in this study is slightly higher than that reported by Hegazy et al. [13]. This study agrees with Hegazy et al. [13] stating that brucellosis is endemic in Egypt with a high seroprevalence (around 15 %) despite the current national control programme. This may be due to poor availability of resources, a lack of ...
Isolated case reports of peritonitis due to Brucella spp. during peritoneal dialysis (PD) continue to surface in the medical literature. However, the optimal treatment regimen for these patients, in particular with regards to the fate of PD catheter, is still largely unknown. We report a case of brucella peritonitis successfully treated with intraperitoneal administration of amikacin, along with oral rifampicin and doxycycline but without catheter removal. Furthermore, we have reviewed the literature up until present day.
ABSTRACT - EPIDEMIOLOGICAL EVALUATION OF A VACCINATION CAMPAIGN AGAINST BOVINE BRUCELLOSIS IN THE REGION OF ALENTEJO WITH THE RB51 VACCINE - Bovine Brucellosis (BB) is a bacterial disease of cattle caused by Brucella abortus, less frequently by B. melitensis and occasionally by B. suis, which can cause disease in humans. It is a notifiable disease with worldwide distribution, responsible for economic losses in countries such as Portugal, where it is endemic. Clinically it is characterized by one or more of the following clinical signs: abortion in the last third of gestation, retained placenta, orchitis, epididymitis and, rarely, arthritis. Infection can occur to offspring in utero or by contact with secretions and excretions of infected animals, with high rates of infectivity and resistance in the environment. The clinical diagnosis does not allow the confirmation of the infection, being necessary to use the laboratory diagnosis. To track the infection, serology tests are often used. Is common ...
Brucella abortus is a Gram-negative, facultative intracellular bacterium that causes brucellosis, a worldwide zoonotic disease leading to undulant fever in humans and abortion in cattle. The immune response against this bacterium relies on the recognition of microbial pathogen-associated molecular patterns, such as lipoproteins, lipopolysaccharides and DNA; however, the immunostimulatory potential of B. abortus RNA remains to be elucidated. Here, we show that dendritic cells (DCs) produce significant amounts of IL-12, IL-6 and IP-10/CXCL10, when stimulated with purified B. abortus RNA. IL-12 secretion by DCs stimulated with RNA depends on TLR7 while IL-6 depends on TLR7 and partially on TLR3. Further, only TLR7 plays a role in IL-12 production induced by B. abortus infection. Moreover, cytokine production in DCs infected with B. abortus or stimulated with bacterial RNA was reduced upon pretreatment with MAPK/NF-κB inhibitors. By confocal microscopy, we demonstrated that TLR7 is colocalized with B.
Brucella abortus causes brucellosis mainly in cattle. The infection is transmitted to humans by ingestion of animal products or direct contact with infected material. While the intracellular lifestyle of Brucella is well characterized, its extracellular survival is poorly understood. In nature, bacterial persistence is associated with biofilms, where aggregated cells are protected from adversity. The inability of Brucella abortus to aggregate under aerobiosis and that fact that the replicative niche of Brucella is characterized by microaerobic conditions prompted us to investigate the capacity of this pathogen to aggregate and grow in biofilms under microaerobiotic conditions. The results show that B. abortus aggregates and produces biofilms. The aggregates tolerate desiccation better than planktonic cells do, adhere and displace even in the absence of the lipopolysaccharide-O antigen, flagella, the transcriptional regulator VjbR, or the enzymes that synthesize, transport, and modify cyclic β ...
Commission Decision 2005/604/EC of 4 August 2005 amending Decision 93/52/EEC as regards the declaration that certain regions of Italy are free of brucellosis (B. melitensis) and Decision 2003/467/EC as regards the declaration that certain provinces of Italy are free of bovine brucellosis and that the region of Piemonte is free of enzootic bovine ...
Although there has been much written about the phenotype of rough mutants, those reports have relied upon the characterization of fortuitous rough isolates. Since nothing was known about the genetic defects in those organisms, any biological properties attributed to the lack of O antigen influenced the interpretation of subsequent experimental observations. For example, Brucella ovis andB. canis are naturally occurring rough species which are pathogenic in their preferred hosts and resistant to complement-mediated lysis (10, 46). As a result, most researchers have maintained that these organisms must express a shortened (somehow undetectable) O antigen (43). However, recent work in our laboratory with the broadly reactive anti-lipid A antibody (MAb 177) described in this paper indicates the presence of LPS in B. ovis and B. canis which is indistinguishable from that observed in the lps mutants (CA353, CA533, and CA613) of B. abortus (data not shown). The differences in phenotype (virulent versus ...
Among animals, brucellosis is transmitted directly (contact with placenta, aborted fetus, the liquor , vaginal and/or uterus secretion) or indirectly (if infected and non-infected health herds mix while grazing or by contact with environment in which the bacteria can be found such as manure, pastures, water, etc). Most infected male animals continue to secrete Brucella via semen for the lifetime. Presence of infectious agents in udder and surrounding lymph nodes results in permanent or occasional secretion of Brucella via milk and as such it is a serious source of infection for young animals which can also be infected via uterus. In infected lambs and young goats, Brucella inhabits lymph nods of bowls and can be secreted out of their body via droppings. Infected young cattle develop the disease once they reached sexual maturity. Sheppard dogs can also spread brucellosis between ruminants but they can also get ill as well. In high humidly, low temperature and unsunny weather conditions, Brucella ...
LOCUS SBW13224.1 61 aa PRT BCT 14-DEC-2016 DEFINITION Brucella sp. 10RB9215 hypothetical protein protein. ACCESSION LT599047-28 PROTEIN_ID SBW13224.1 SOURCE Brucella sp. 10RB9215 ORGANISM Brucella sp. 10RB9215 Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Brucellaceae; Brucella; unclassified Brucella. REFERENCE 1 AUTHORS Blom J. JOURNAL Submitted (05-JUL-2016) to the INSDC. JLU, JLU, Justus-Liebig-University Giessen, Bioinformatics and Systems Biology, Heinrich-Buff-Ring 58, 35392 Giessen, 35390, Germany FEATURES Qualifiers source /organism=Brucella sp. 10RB9215 /chromosome=1 /host=African bull frog, Pyxicephalus edulis /isolate=BR10RB9215WGS1 /mol_type=genomic DNA /collection_date=2009 /db_xref=taxon:1149953 protein /transl_table=11 /locus_tag=BR10RB9215_C10028 /db_xref=UniProtKB/TrEMBL:A0A1M4L950 BEGIN 1 MEGQVIFYKN DRIIYRHHID VQEDDYSKGV NDALIAFQRN YAGFDLASDD IHIRFKKPGD 61 V ...
Brucellosis, Undulant fever, Mediterranean fever or Malta fever is different wards for one disease which is an infectious disease caused by a type of bacteria called Brucella , that are almost invariably transmitted by direct or indirect contact with infected animals or their products. Additionally, human brucellosis has a serious medical impact worldwide, which affects people of all age groups and of both sexes. The eradication poses major difficulties because this disease is largely under diagnosed and underreported. Furthermore, brucellosis in human beings is rarely fatal; it can be severely debilitating and disabling. A renewed scientific interest in human brucellosis has been fuelled by its recent re-emergence and enhanced surveillance in many areas of the world because of regarding the disease as a class B bioterrorist agent and remains the world?s most common bacterial zoonosis. A cross-sectional study was conducted in the period from March, 2013 to March, 2014 to estimate seroprevalence ...
Brucellosis is primarily a worldwide zoonotic disease caused by Brucella species. The genus Brucella contains highly infectious species that are classified ...
Interpretive Summary: Brucella are intracellular pathogens that cause reproductive losses in cattle and zoonotic infections in people. Live attenuated vaccines have been available for protecting domestic livestock against brucellosis for more than 60 years. Current vaccines are effective in preventing abortion and transmission of brucellosis, but poor at preventing infection or seroconversion. Current knowledge suggests that both the innate and adaptive immune responses contribute to immunity against intracellular pathogens and that binding of pathogen structures onto pattern recognition receptors (PMRs) is critical to development of protective immunity. The observations that killed bacteria or subunit vaccines do not appear to fully stimulate PMRs or mimic intracellular trafficking of live Brucella, may explain their inability to induce protection that equals that provided by live vaccines. Brucella appear to have multiple mechanisms that subvert innate and adaptive immunity and prevent or ...
Brucellosis is one of the most important infectious diseases worldwide. Nevertheless, since it is not regarded as a priority by national and international health systems in many endemic regions, it is considered to be a neglected zoonosis. Measures to prevent and control brucellosis rely upon direct approaches aimed at minimising the risk of spreading infection among animals. Collectively, these measures tend to reduce the exposure of animals to Brucella spp. and to increase resistance to infection in susceptible animals. To implement an effective disease control strategy, detailed information about the presence of the pathogen in a specific territory is of fundamental importance. For that reason, particular emphasis should be placed on active surveillance using serological methods. Serological surveillance provides useful information to aid in understanding epidemiological patterns and assess the impact of brucellosis in the targeted area, paving the way to define the most suitable approaches ...
Disrupting the ure1 operon causes loss of urease activity, decreased resistance to low pH killing in vitro and decreased pathogen survival when inoculated in BALB/c mice by gavage.
Antibiotics such as tetracyclines, rifampicin, and the aminoglycosides streptomycin and gentamicin are effective against Brucella bacteria. However, the use of more than one antibiotic is needed for several weeks, because the bacteria incubate within cells.. Surveillance using serological tests, as well as tests on milk like the milk ring test, can be used for screening and play an important role in campaigns to eliminate the disease. As well individual animal testing both for trade and for disease control purposes is practiced. In endemic areas, vaccination is often used to reduce the incidence of infection. Several vaccines are available that use modified live viruses. The World Organisation for Animal Health Manual of Diagnostic Test and Vaccines for Terrestrial Animals provides detailed guidance on the production of vaccines.As the disease is closer to being eliminated, a test and stamping out program is required to completely eliminate it.. The gold standard treatment for adults is daily ...
Brucellosis is a zoonosis or a disease that can be transmitted from animals to humans. People can also get the disease by eating products from infected animals. It is also known as undulant fever or Mediterranean fever.
PANAMA – National Animal Health Officials are baffled as to the source of a Brucellosis outbreak centred on the western half of the country’s capital.
COLOMBIA – Advances are being made in delivering a tuberculosis and brucellosis ‘free’ programme to a southern region of the country, says the Colombian Agricultural Institute.
SPAIN – After 20 years of eradication efforts, four autonomous areas have been officially declared bovine Brucellosis free.
JE Hyung Gon , SONG Hyun Circulation journal : official journal of the Japanese Circulation Society 72(3), 500-501, 2008-02-20 J-STAGE 医中誌Web 参考文献8件 ...
Using serology and bacterial culture, we determined the prevalence of Brucella spp. and the antibody to Brucella spp. in a feral swine (Sus scrofa) population in proximity to a cattle herd that was culture positive for Brucella abortus and Brucella suis in north-central Texas, USA. During a prospective cross-sectional quantitative study in April 2005, we collected blood and tissue samples from 40 feral swine within a 30-km radius of the infected herd. Serum samples were tested by the Rose Bengal test, particle concentration fluorescence immunoassay, and fluorescence polarization assay. In addition, tissue samples were cultured, and the Brucella species and biovar determined. Four feral swine were Brucella positive by serology, and two were culture positive for B. suis biovar 1. Of the culture-positive swine, one was concurrently antibody and culture positive, and one was culture positive only. The presumptive source of the B. suis infection in the index cattle herd was likely the surrounding feral swine
Brucellosis is a zoonotic bacterial disease caused by Brucella spp. The virulence of these bacteria is dependent on their ability to invade and replicate within host cells. In a previous study, a putative gene bab_RS27735 encoding an amino acid ABC transporter substrate-binding protein homologous to AapJ protein was found to be involved in Brucella abortus virulence. In this study, we successfully constructed a bab_RS27735 deletion mutant, Δ27735. Compared with the wild-type strain, the lipopolysaccharide pattern of the mutant was not changed, but the growth ability was slightly defected in the exponential phase. In tolerance tests, sensitivity of the Δ27735 mutant to oxidative stress, bactericidal peptides or low pH was not different from that of the wild-type strain. Cell infection assay showed that the mutant was reduced survival within macrophages but could efficiently escape lysosome degradation. The results of a virulence test showed that the Δ27735 mutant was attenuated in a mouse model at the
Objective To study antimicrobial resistance of Brucella melitenesis and instruct rational use of antimicrobial agents for clinic.Methods Bacteria were cultured and identified by Bact/Alert 3D and VITEK Ⅱ automicrobic system.Drug susceptibility was detected by E-test method.Results Four strains Brucella melitenesis were isolated from blood of 4 patients with fever and MIC of gentamicin,ampicillin and ciprofloxacin was(0.5)~(0.75 mg/L),(1.5)~(2.0 mg/L) and (4.0)~(8.0 mg/L) respectively.MIC of ceftazidime,amikacin and doxycycline were all above(8.0 mg/L).Conclusion The preferred drug for treating brucellosis is gentamicin or plus ampicillin or ciprofloxacin,or choosing antimicrobial agents according to antimicrobial susceptibility test results.
Brucellosis is a serious public health issue in India. Estimation of economic losses occurring due to brucellosis is required to help formulate prevention and control strategies, but has not been done in India. We estimated economic losses due to brucellosis by sourcing prevalence data from epidemiological surveys conducted in India. Data for livestock populations were obtained from official records. Probability distributions were used for many of the input parameters to account for uncertainty and variability. The analysis revealed that brucellosis in livestock is responsible for a median loss of US $ 3.4 billion (5th-95th percentile 2.8-4.2 billion). The disease in cattle and buffalo accounted for 95.6% of the total losses occurring due to brucellosis in livestock populations. The disease is responsible for a loss of US$ 6.8 per cattle, US$18.2 per buffalo, US$ 0.7 per sheep, US$ 0.5 per goat and US$ 0.6 per pig. These losses are additional to the economic and social consequences of the ...
Neutralophilic bacteria have developed specific mechanisms to cope with the acid stress encountered in environments such as soil, fermented foods- and host compartments. In Escherichia coli, the glutamate decarboxylase (Gad)-dependent system is extremely efficient: it requires the concerted action of glutamate decarboxylase (GadA/GadB) and of the glutamate/GABA antiporter, GadC. Notably, this system is operative also in new strains/species of Brucella, among which Brucella microti, but not in the
"Erythritol triggers expression of virulence traits in Brucella melitensis". Microbes and Infection. 15 (6-7): 440-449. doi: ... Erythritol is preferentially utilized by the Brucella bacteria spp. The presence of erythritol in the placentas of goats, cows ... and pigs has been proposed as an explanation for the accumulation of Brucella bacteria found at these sites. In the 19th and ...
2015). "Recovery of a medieval Brucella melitensis genome using shotgun metagenomics". MBio. 5: e01337-14. doi:10.1128/mBio. ... recovering 200-year-old Mycobacterium tuberculosis genomes from human remains and a medieval Brucella genome sequence. With ...
Professor Debono spent much time studying the brucella melitensis strain of brucellosis. In 1939 Debono assisted with the ...
Brucella melitensis is the cause of undulant fever in man and of abortion in goats. It is usually transmitted by goat's milk. ... later renamed Brucella melitensis). Themistocles Zammit, one of the members of the commission, discovered the reason behind ... Brucella is the genus and Brucellaceae is the family of the bacteria which was named after him, in recognition of his ... he led the Malta Fever Commission that identified the organism that caused the fever as a bacterium Micrococcus melitensis ( ...
Since brucellosis threatens the food supply and causes undulant fever, Brucella suis and other Brucella species (B. melitensis ... of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella ... DelVecchio, VG; Kapatral, V; Elzer, P; Patra, G; Mujer, CV (20 December 2002). "The genome of Brucella melitensis". Veterinary ... Moreno, E; Moriyon, I (8 January 2002). "Brucella melitensis: a nasty bug with hidden credentials for virulence". Proceedings ...
These materials included anthrax, West Nile virus and botulism, as well as Brucella melitensis, and Clostridium perfringens. ... and new research activities involving Brucella and Crimean-Congo hemorrhagic fever virus. Despite diligent investigations since ...
Moraxella catarrhalis, Brucella melitensis, Chlamydia pneumoniae, and Mycoplasma pneumoniae are generally susceptible to ... ducreyi Plague due to Yersinia pestis Tularemia Cholera Campylobacter fetus infections Brucellosis caused by Brucella species ( ...
Brucella abortus (Q57B94), Brucella suis (Q8FYS0) and Brucella melitensis (Q8YJ29). The biochemical mechanism of proline ...
"Establishment of systemic Brucella melitensis infection through the digestive tract requires urease, the type IV secretion ... Ugalde, J.E.; Comerci, D.J.; Leguizamon, M.S.; Ugalde, R.A. (2003). "Evaluation of Brucella abortus phosphoglucomutase (pgm) ... Ugalde, J.E.; Czibener, C.; Feldman, M.F.; Ugalde, R.A. (2000). "Identification and characterization of the Brucella abortus ... Pei, J.; Ficht, T.A. (2004). "Brucella abortus rough mutants are cytopathic for macrophages in culture". Infection and Immunity ...
... of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella ... 2002). "The genome sequence of the facultative intracellular pathogen Brucella melitensis". Proc. Natl. Acad. Sci. U.S.A. 99 (1 ... 2002). "The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts". Proc. Natl ... 2005). "Whole-genome analyses of speciation events in pathogenic Brucellae". Infection and Immunity. 73 (12): 8353-61. doi: ...
... aeruginosa Staphylococcus aureus Staphylococcus epidermidis Streptococcus agalactiae Streptococcus uberis Brucella melitensis ...
Brucella melitensis is the cause of undulant fever in man and of abortion in goats. It is usually transmitted by goat's milk. ... later renamed Brucella melitensis).[10] Themistocles Zammit, one of the members of the commission, discovered the cause of the ... Corbel, M.J.; Banai, M. (2015). "Brucella". Bergey's Manual of Systematics of Archaea and Bacteria. John Wiley & Sons. pp. 1-30 ... Brucella is the genus and Brucellaceae is the family of the bacteria which was named after him, in recognition of his ...
The facility was one of the first in Peru to be able to work with BSL-3 agents such as Brucella melitensis, Yersinia pestis and ...
... abortus A13334, 3,401 ORFs Brucella canis ATCC 23365, 3,408 ORFs Brucella melitensis 16M, 3,279 ORFs Brucella microti ... 3,193 ORFs Brucella pinnipedialis B2/94, 3,505 ORFs Brucella suis 1330, 3,408 ORFs Genome data for these and other Brucella ... Sir David Bruce isolated B. melitensis from British soldiers who died from Malta fever in Malta. After exposure to Brucella, ... Hence, the NCBI taxonomy includes most Brucella species under B. melitensis. The many names of brucellosis include (human ...
Botulinum neurotoxin Botulinum neurotoxin producing species of Clostridium Brucella abortus Brucella melitensis Brucella suis ...
... infected with Brucella melitensis and with occupational exposure of laboratory workers, veterinarians, and slaughterhouse ... Brucella species survive well in aerosols and resist drying. Brucella and all other remaining biological weapons in the U.S. ... B. abortus is less virulent than B. melitensis and is primarily a disease of cattle. B. canis affects dogs. B. melitensis is ... The growth of brucellae is extremely slow (they can take up to two months to grow) and the culture poses a risk to laboratory ...
Bordetella bronchiseptica Bordetella pertussis Borrelia burgdorferi Brucella Brucella abortus Brucella melitensis Brucella suis ...
Brucella abortus Brucella melitensis Brucella suis Burkholderia mallei* (formerly Pseudomonas mallei) Burkholderia pseudomallei ...
Complete ORF sets have been cloned for a number of organisms including Brucella melitensis Chlamydia pneumoniae Escherichia ... "Construction and evaluation of an ORFeome-based Brucella whole-genome DNA microarray". Microbial Pathogenesis. 47 (4): 189-95. ...
... is a Gram-negative coccobacillus from the Brucellaceae family. Along with Brucella melitensis, it is responsible ... Brucella ovis, reviewed and published by Wikivet at http://en.wikivet.net/Brucella_ovis, accessed 24/08/2011.. ...
sRNA BM-sr0117 from Brucella melitensis was identified and shown to be bound to and cleaved by Bm-RNase III. AbcR and AbcR2 ( ... In Brucella melitensis eight novel sRNA genes were identified using bioinformatic and experimental approach. One of them ... 2015). "Identification of a Novel Small Non-Coding RNA Modulating the Intracellular Survival of Brucella melitensis". Front. ... They are often bound to the chaperone protein Hfq, which allows them to interact with mRNA(s). In Brucella suis 1330 RNA ...
... en.wikivet.net/Brucella_melitensis, accessed 2011-08-24. Brucella melitensis genomes and related information at PATRIC, a ... Bioinformatics Resource Center funded by NIAID Brucella melitensis 16M genome information Brucella melitensis in African ... Brucella melitensis is a Gram-negative coccobacillus bacterium from the Brucellaceae family. The bacterium causes ovine ... Brucella melitensis, reviewed and published by Wikivet at http:// ... melitensis was isolated in Malta by David Bruce from the spleen ...
Brucella melitensis MeSH B03.440.400.425.215.500.625 --- Brucella ovis MeSH B03.440.400.425.215.500.750 --- Brucella suis MeSH ... Brucella melitensis MeSH B03.660.050.070.100.625 --- Brucella ovis MeSH B03.660.050.070.100.750 --- Brucella suis MeSH B03.660. ... Brucella MeSH B03.440.400.425.215.500.100 --- Brucella abortus MeSH B03.440.400.425.215.500.150 --- Brucella canis MeSH B03.440 ... Brucella MeSH B03.660.050.070.100.100 --- Brucella abortus MeSH B03.660.050.070.100.150 --- Brucella canis MeSH B03.660.050.070 ...
9-941 chromosome I (NC_006932), Bmar7CI = Brucella melitensis biovar Abortus 2308 chromosome I (NC_007618), Bor7CI = Brucella ... 16M chromosome I (NC_003317), BaS19r7CI = Brucella abortus S19 chromosome 1 (NC_010742), Bm23457r7CI = Brucella melitensis ATCC ... Brucella suis ATCC 23445 chromosome I (NC_010169), Bm16Mr7CI = Brucella melitensis bv. 1 str. ... Partial synteny of the αr7 genomic regions was observed in the Mesorhizobium and Brucella group, where instead of the MarR ...
viciae 3841 strain, and the Agrobacterium species A. vitis,A. tumefaciens, A. radiobacter and A. H13, Brucella species (B. ovis ... melitensis), in Bartonella species (i.e. B. henselae, B. clarridgeiae, B. tribocorum, B. quintana, B. bacilliformis, B. ... as well as in a broad spectrum of Brucella species (B. ovis, B. canis, B. abortus and B. microtis, and several viobars of B. ... Partial synteny of the αr45 genomic regions was observed in the Mesorhizobium and Brucella species where instead of aLysR ...
Brucellosis is caused by different biotypes of Brucella abortus and B. melitensis.[52] Other internal parasites include ...
... from the smooth Brucella abortus strain 99 and the smooth B. melitensis strain 16M and thus recognize epitopes present on the O ... melitensis strain B115 and whole B. melitensis B115 cells. Surprisingly, 22 monoclonal antibodies (mAbs) reacting in ELISA with ... melitensis strain B115 by immunoelectron microscopy. Immunogold labelling was observed at the surface of B. melitensis B115 ... These results indicate that O-chain expressed in the rough B. melitensis strain B115 is immunogenic in mice, not exposed at the ...
... en.wikivet.net/Brucella_melitensis, accessed 2011-08-24. Brucella melitensis genomes and related information at PATRIC, a ... Bioinformatics Resource Center funded by NIAID Brucella melitensis 16M genome information Brucella melitensis in African ... Brucella melitensis is a Gram-negative coccobacillus bacterium from the Brucellaceae family. The bacterium causes ovine ... Brucella melitensis, reviewed and published by Wikivet at http:// ... melitensis was isolated in Malta by David Bruce from the spleen ...
Brucella melitensis bv. 3 str. Ether cont1.42, whole genome shotgun sequence Brucella melitensis bv. 3 str. Ether cont1.42, ... Brucella melitensis bv. 3 str. Ether cont1.42, whole genome shotgun sequence. GenBank: ACEI01000042.1 ...
Sulfur metabolism - Brucella melitensis M28 [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show description ...
... Sarwar Azam,1 Sashi ... Indels identified in the genome of Bm IND1 with respect to B. melitensis ATCC 23457. Indels were extracted using raw reads of ... Bm IND1 aligned to B. melitensis ATCC 23457 reference genome with the help of Bowtie2 and Samtools. SnpEFF was used to detect ...
... melitensis 16M, B. melitensis M28, B. melitensis ATCC 23457, B. melitensis M5-90, B. melitensis NI, B. melitensis Ether, and B. ... melitensis M28, B. melitensis ATCC 23457, B. melitensis M5-90, B. melitensis NI, B. melitensis Ether, and B. abortus 2308 for ... melitensis M28, B. melitensis M5-90, and B. melitensis NI, whereas B. melitensis Ether and B. melitensis 16M branched ... Completely sequenced genomes of B. melitensis such as B. melitensis 16M, B. melitensis M28, B. melitensis ATCC23457, and B. ...
... SKU: GL-1002 Retail Price: $24.50. Our LOW Price: $18.33. You Save: $6.17 (25%). Availability: ... Home » Medical Equipment & Supplies » Instruments & Disposables » Urine Reagent Strips, Individual Packs » Brucella Melitensis ...
... of the vegetation with the echocardiography and with the production from the multiple blood culture of brucella melitensis and ... We present a rare case of brucella endocarditis, forming a vegetation on the mitral valve. The definitive diagnosis has been ...
What is Brucella melitensis? Meaning of Brucella melitensis medical term. What does Brucella melitensis mean? ... Looking for online definition of Brucella melitensis in the Medical Dictionary? Brucella melitensis explanation free. ... Related to Brucella melitensis: Brucella canis, Brucella abortus, Brucella suis. Bru·cel·la me·li·ten·sis. (brū-selă mel-i- ... Brucella melitensis as causative agent for neck abscess in an endemic area.. Brucella Melitensis As Causative Agent for Neck ...
Secondary bile acid biosynthesis - Brucella melitensis ATCC 23457 [ Pathway menu , Organism menu , Pathway entry , Download ...
Immunogenicity evaluation of plasmids encoding Brucella melitensis Omp25 and Omp31 antigens in BALB/c mice.. Shojaei M1, ... Immunogenicity evaluation of plasmids encoding Brucella melitensis Omp25 and Omp31 antigens in BALB/c mice ... Immunogenicity evaluation of plasmids encoding Brucella melitensis Omp25 and Omp31 antigens in BALB/c mice ... Immunogenicity evaluation of plasmids encoding Brucella melitensis Omp25 and Omp31 antigens in BALB/c mice ...
Recovery of a Medieval Brucella melitensis Genome Using Shotgun Metagenomics. Gemma L. Kay, Martin J. Sergeant, Valentina ... Recovery of a Medieval Brucella melitensis Genome Using Shotgun Metagenomics. Gemma L. Kay, Martin J. Sergeant, Valentina ... Recovery of a Medieval Brucella melitensis Genome Using Shotgun Metagenomics. Gemma L. Kay, Martin J. Sergeant, Valentina ... Recovery of a Medieval Brucella melitensis Genome Using Shotgun Metagenomics Message Subject (Your Name) has forwarded a page ...
Presence of two independent chromosomes in the Brucella melitensis 16M genome.. S Michaux, J Paillisson, M J Carles-Nurit, G ... Presence of two independent chromosomes in the Brucella melitensis 16M genome.. S Michaux, J Paillisson, M J Carles-Nurit, G ... Presence of two independent chromosomes in the Brucella melitensis 16M genome.. S Michaux, J Paillisson, M J Carles-Nurit, G ... Presence of two independent chromosomes in the Brucella melitensis 16M genome. Message Subject (Your Name) has forwarded a page ...
Study of the organization of the genomes of Escherichia coli, Brucella melitensis and Agrobacterium tumefaciens by insertion of ... Brucella melitensis and Agrobacterium tumefaciens C58, which all lack natural I-SceI sites in their genomes. Digestion of the ... melitensis and of a circular and a linear chromosome in A. tumefaciens C58 (which also contains two large circular plasmids). ...
We isolated Brucella melitensis biovar 3 from the spleen of an Asian badger (Meles leucurus) in Nilka County, northwestern ... Brucella antigen in liver and spleen from Asian badgers infected with Brucella melitensis, northwestern China. A) Brucella ... Brucella antigen in liver and spleen from Asian badgers infected with Brucella melitensis, northwestern China. A) Brucella ... Wang Q, Zhao S, Wureli H, Xie S, Chen C, Wei Q, et al. Brucella melitensis and B. abortus in eggs, larvae and engorged females ...
The predominant Brucella species in both animal and human infections is Brucella melitensis [4]. A vaccine for B. melitensis ... "Oral vaccination with Brucella melitensis WR201 protects mice against intranasal challenge with virulent Brucella melitensis ... CFU concentration was verified by serial dilution and plating on Brucella agar. Rough Brucella lysate (RFBL) and Brucella LPS ... S. L. Yingst, The role of the CD8+ T cell in the immune response to Brucella melitensis in mice [Ph.D. thesis], Department of ...
1 and H38 Brucella melitensis vaccines. Brucella melitensis. CEC seminar, Brussels, November 1984, 215-227; [Current Topics in ... Brucella melitensis in Greece: current situation report. Brucella melitensis. CEC seminar, Brussels, November 1984, 43-46; [ ... Brucella melitensis mainly infects sheep and goats, and in areas where B. melitensis is enzootic it is the major cause of ... Brucella melitensis primarily affects the reproductive tract of sheep and goats, and B. melitensis infection is characterized ...
We isolated Brucella melitensis biovar 3 from the spleen of an Asian badger (Meles leucurus) in Nilka County, northwestern ... Brucella antigen in livers and spleens from Asian badgers infected with Brucella melitensis, northwestern China. A) Brucella ... Brucella melitensis in Asian Badgers, Northwestern China Xiafei Liu1, Meihua Yang1, Shengnan Song1, Gang Liu, Shanshan Zhao, ... 2; B) liver of an uninfected Asian badger; C) Brucella antigen in spleen of Asian badger no. 2; D) Spleen of badger without ...
It is necessary for persistence in mice, essential for intracellular growth of Brucella and required for expression of the type ... IV secretion system VirB and therefore plays a role in adaptation of Brucella to its intracellular host environment. ... "The stringent response mediator Rsh is required for Brucella melitensis and Brucella suis virulence, and for expression of the ... "The stringent response mediator Rsh is required for Brucella melitensis and Brucella suis virulence, and for expression of the ...
Brucella canis (strain ATCC 23365 / NCTC 10854). Brucella melitensis biotype 2 (strain ATCC 23457). Brucella suis (strain ATCC ... Brucella canis (strain ATCC 23365 / NCTC 10854). Brucella melitensis biotype 2 (strain ATCC 23457). Brucella suis (strain ATCC ... Brucella suis biovar 1 (strain 1330). Brucella sp. 10RB9215. Brucella suis. Brucella abortus bv. 5 str. B3196. And more. 158. ... Brucella abortus (strain S19). Brucella abortus (strain 2308). Brucella abortus biovar 1 (strain 9-941). ...
5-Tetrahydropyridine-2-carboxylate N-Succinyltransferase from Brucella melitensis biovar abortus 2308 ... Crystal structure of 2,3,4,5-Tetrahydropyridine-2-carboxylate N-Succinyltransferase from Brucella melitensis biovar abortus ...
Rapid identification of Yersinia pestis and Brucella melitensis by chip-based continuous flow PCR Author(s): Michael Dietzsch; ... To combat the threat of biological agents like Yersinia pestis and Brucella melitensis in bioterroristic scenarios requires ... a system for rapid amplification of specific genetic markers for the identification of Yersinia pestis and Brucella melitensis ...
Brucella serum (Remel Inc., Lenexa, Kans.). Strains identified as B. melitensis were stored in brucella broth (Remel Inc.) at − ... Brucella strains were isolated from 16 (1.24%) of the cultures, and all of them were identified as B. melitensis. Nine (56.25 ... Synergy Tests by E Test and Checkerboard Methods of Antimicrobial Combinations against Brucella melitensis. Gani Orhan, Aysen ... Synergy Tests by E Test and Checkerboard Methods of Antimicrobial Combinations against Brucella melitensis ...
In 2011, Brucella melitensis biovar 1, a biotype not reported in Italy since 1995, was isolated in one flock in the region. The ... MLVA as an Epidemiological Tool To Trace Back Brucella melitensis Biovar 1 Re-Emergence in Italy.. [F De Massis, M Ancora, M ... The knowledge of Brucella spp. prevailing genotypes in a territory represents an important epidemiological tool to formulate ... Brucellosis is an important zoonosis caused by Brucella spp., still prevalent in most areas of the world. Brucellosis control ...
FARINA, R. Current serological methods in B. melitensis diagnosis. In: PLOMMET, M., VERGER, J.M. (Eds). Brucella melitensis. ... 1 de B. melitensis.. Neste estudo, utilizou-se a vacina viva da estirpe Rev.1 de B. melitensis contendo 1´ 109 UFC/dose vacinal ... Evaluation of serological tests for the diagnosis of Brucella melitensis infection of goats. J. Clin. Microbiol., v.32, p.1159- ... A brucelose caprina e ovina causada pela espécie Brucella melitensis é uma doença infecciosa que se caracteriza por aborto e ...
  • Antibody to the O-polysaccharide of Brucella LPS has been firmly established as an important mediator of anti- Brucella effects in murine models of secondary immunity [ 13 , 14 ]. (hindawi.com)
  • He was initially started on intravenous vancomycin and tazobactam/piperacillin, However, cultures of blood and bursal fluid subsequently grew Brucella melitensis , and the antibody titer to brucella was positive at 1:2560. (shmabstracts.com)
  • The properties of TBG as a fluorogenic substrate for HRP and its application in a fluorometric enzyme-linked immunosensing system were compared with commercially available substrates such as p -hydroxyphenylpropionic acid (pHPPA), chavicol and Amplex red using Brucella melitensis antibody (BrAb) as a model analyte. (mdpi.com)
  • B . melitensis contains more than 3000 genes that are distributed over two circular chromosomes. (hindawi.com)
  • We therefore conducted a systematic disruption strategy for genes putatively coding for transcriptional regulators in B. melitensis 16M. (asm.org)
  • In particular, a total of 25 target genes are involved in regulating apoptosis and autophagy, indicating that these miRNAs may play important regulatory roles in the Brucella -host interactions. (ijbs.com)
  • Therefore, to investigate the relationship between Brucella melitensis 16M and SUMO, we constructed plasmids and cells lines suitable for overexpression and knockdown of SUMO1 and Ubc9 genes. (bvsalud.org)
  • 8. Cloeckaert A, Verger J-M, Grayon M, Grepinet O. Restriction site polymorphism of the genes encoding the major 25 kDa and 36 kDa outer-membrane proteins of Brucella. (ac.ir)
  • The identification of two protective DNA vaccines from a panel of five plasmid constructs encoding Brucella melitensis 16M genes. (ac.ir)
  • Specific genes and biological processes identified in this study will further help elucidate how both host and Brucella interact during the early infectious process to the eventual benefit of the pathogen and to the detriment of the naïve host. (omicsdi.org)
  • In THP-1 macrophage infected with Brucella melitensis we studied the expression levels of four host's genes, i.e. (ac.ir)
  • Our data showed that at this molecular level, except for FRMD5 that was downregulated, the other three genes were upregulated by B. melitensis. (ac.ir)
  • Brucella melitensis and M. tuberculosis go through similar intracellular processes and interestingly two of the investigated genes, i.e. (ac.ir)
  • In Brucella melitensis eight novel sRNA genes were identified using bioinformatic and experimental approach. (wikipedia.org)
  • Brucella mechanisms and virulence factors that mediate invasion and intracellular persistence have been poorly characterized. (hindawi.com)
  • Since rough mutants of B. melitensis are protected from extracellular complement-mediated killing, we decided to investigate whether the attenuation in virulence previously observed in these strains was associated with differences in their interaction with macrophages. (asm.org)
  • Some transcriptional regulators of Brucella that have a role in the control of virulence have already been identified. (asm.org)
  • Some transcriptional regulators required for Brucella 's virulence were also identified in several screens in cellular models and in BALB/c mice ( 19 , 31 , 35 , 36 ). (asm.org)
  • Brucella strains express uncanonical virulence factors including lipopolysaccharides (LPS), virulence regulator proteins and phosphatidylcholine, but lack most of classical virulence factors such as invasive protease, extoxin, or virulence plasmids [ 3 ]. (ijbs.com)
  • To confirm attenuated virulence several of the mutants were evaluated for survival in the mouse splenic clearance model using a 1:1 mixture of parental wild-type ( B. melitensis 16 M) and mutant to inoculate mice (n = 10 per group) with an overall dose of 5 × 10 6 cfu. (biomedcentral.com)
  • We performed multilocus sequence typing of four B . melitensis strains isolated from naturally infected goats from India. (hindawi.com)
  • Two different synergy testing methods, the checkerboard and the E test methods, were used to compare the in vitro efficacies of various antimicrobial combinations against 16 Brucella melitensis strains isolated from blood cultures. (asm.org)
  • Phenotypic and genotypic characterisation of Brucella strains isolated from cattle in the Gambia. (ebscohost.com)
  • This is the first report of a detailed genetic investigation of an extensive collection of B. melitensis strains isolated from human cases in Germany. (uni-muenchen.de)
  • The aim of this study was the first genetic analysis of B. melitensis strains isolated from dromedary camels (Camelus dromedarius) using multiple-locus variable-number tandem repeat analysis (MLVA). (cdc.gov)
  • In this work, a patient living in Shanxi Province in China who had suffered a spontaneous abortion underwent pathogen detection to analyse the genetic characteristics of the spontaneous abortion-related Brucella strain. (biomedcentral.com)
  • It is necessary for persistence in mice, essential for intracellular growth of Brucella and required for expression of the type IV secretion system VirB and therefore plays a role in adaptation of Brucella to its intracellular host environment. (uniprot.org)
  • Spleen cells from mice infected with the rough Brucella melitensis strain B115 were fused with NSO myeloma cells. (microbiologyresearch.org)
  • These results indicate that O-chain expressed in the rough B. melitensis strain B115 is immunogenic in mice, not exposed at the cell surface but present in the cytoplasm and most probably at the cytoplasmic membrane. (microbiologyresearch.org)
  • Survey of Protection of Recombinant Cell Surface Protein 31kDa from Brucella melitensis in BALB/c Mice. (ac.ir)
  • At one (white bars) and four weeks (black bars) post inoculation spleens were excised from the mice, and recovery of the B. melitensis 16 M and the mutant were determined as described in the Materials and Methods. (biomedcentral.com)
  • We tested putative Brucella colonies by using H 2 S production, dye inhibition, agglutination by monospecific serum, and sensitivity to bacteriophages ( Appendix Table). (cdc.gov)
  • Gram-negative, non-spore-forming, nonmotile coccobacilli which were oxidase and urease positive were tested for agglutination with monospecific anti- Brucella serum (Remel Inc., Lenexa, Kans. (asm.org)
  • To determine the role of surface OPS in the interactions of B. melitensis with monocytes/macrophages (M/M), 16M and WRR51 were transformed with the plasmid pBBR1MCS-6y encoding green fluorescent protein, and the transformants were used to infect human mononuclear phagocytes with and without fresh human serum as a source of complement. (asm.org)
  • Antibodies to recombinant TOB (rTOB) have been investigated in Brucella-infected human sera and a pool serum prepared from B. melitensis -vaccinated rabbits. (ac.ir)
  • In this study, we generated and characterized 22 novel murine monoclonal antibodies (mAbs) binding native Omp31 of B. melitensis . (biomedcentral.com)
  • L'objectif de cette étude est le clonage, l'analyse et le séquençage du gène Omp31 ainsi que la prédiction des épitopes de l'antigène majeur de B. melitensis. (ac.ir)
  • Complete ORF sets have been cloned for a number of organisms including Brucella melitensis Chlamydia pneumoniae Escherichia coli Neisseria gonorrhoeae Pseudomonas aeruginosa Schizosaccharomyces pombe Staphylococcus aureus human herpesviruses A partial human ORFeome has also been produced. (wikipedia.org)
  • These digests also gave direct and independent proof for the single circular chromosome of E. coli, and for the presence of two circular chromosomes in B. melitensis and of a circular and a linear chromosome in A. tumefaciens C58 (which also contains two large circular plasmids). (nih.gov)
  • The solubility, cytotoxicity, and efficacy of these nano-packed antibiotics on Brucella -infected murine phagocytic cells were examined, as compared with free antibiotics. (ac.ir)
  • however, the efficacy of rifampicin on Brucella -infected murine phagocytic cells was enhanced. (ac.ir)
  • Genotypes III, IV and V of B. melitensis demonstrated a restricted geographical distribution, with genotype III representing the African lineage, genotype IV representing the European lineage and genotype V representing the American lineage. (biomedcentral.com)
  • 4. Avila-Calderon ED, Lopez-Merino A, Sriranganathan N, Boyle SM, Contreras-Rodriguez A. A history of the development of Brucella vaccines. (ac.ir)
  • By sequencing DNA extracted from medieval human remains through shotgun metagenomics, without target-specific capture or amplification, we have obtained a draft genome sequence of an ~700-year-old Brucella melitensis strain. (asm.org)
  • Upon invasion, Brucella utilizes vesicles formed by macrophage endocytosis to transport and block the fusion between Brucella -containing vesicles and lysosomes [ 6 , 7 ]. (ijbs.com)
  • We previously showed that a purE mutant (delta purE201) of Brucella melitensis 16M is attenuated for growth in cultured human monocytes (E. S. Drazek, H. H. Houng, R. M. Crawford, T. L. Hadfield, D. L. Hoover, and R. L. Warren, Infect. (asm.org)
  • Recovery of Brucella is presented as a Survival Ratio (CFU 16 M/CFU mutant) corrected for variation in the input ratio in the inoculum calculated the same way. (biomedcentral.com)
  • Brucella invades and replicates in professional phagocytic cells such as macrophages and dendritic cells as well as nonprofessional phagocytes such as trophoblasts [ 3 - 5 ]. (hindawi.com)