A species of the genus BRUCELLA whose natural hosts are sheep and goats. Other mammals, including humans, may be infected. In general, these organisms tend to be more virulent for laboratory animals than BRUCELLA ABORTUS and may cause fatal infections.
Infection caused by bacteria of the genus BRUCELLA mainly involving the MONONUCLEAR PHAGOCYTE SYSTEM. This condition is characterized by fever, weakness, malaise, and weight loss.
A genus of gram-negative, aerobic bacteria that causes BRUCELLOSIS. Its cells are nonmotile coccobacilli and are animal parasites and pathogens. The bacterium is transmissible to humans through contact with infected dairy products or tissue.
A species of the genus BRUCELLA whose natural hosts are cattle and other bovidae. Abortion and placentitis are frequently produced in the pregnant animal. Other mammals, including humans, may be infected.
A bacterial vaccine for the prevention of brucellosis in man and animal. Brucella abortus vaccine is used for the immunization of cattle, sheep, and goats.
A species of the genus BRUCELLA which are pathogenic to SHEEP.
A species of gram-negative bacteria, primarily infecting SWINE, but it can also infect humans, DOGS, and HARES.
Diseases of domestic and mountain sheep of the genus Ovis.
A disease of cattle caused by bacteria of the genus BRUCELLA leading to abortion in late pregnancy. BRUCELLA ABORTUS is the primary infective agent.
Any of numerous agile, hollow-horned RUMINANTS of the genus Capra, in the family Bovidae, closely related to the SHEEP.
A bright bluish pink compound that has been used as a dye, biological stain, and diagnostic aid.
Diseases of the domestic or wild goat of the genus Capra.
A species of gram-negative bacteria infecting DOGS, the natural hosts, and causing canine BRUCELLOSIS. It can also cause a mild infection in humans.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
A synthetic tetracycline derivative with similar antimicrobial activity.
Substances elaborated by bacteria that have antigenic activity.
Antibodies that are chemically bound to a substrate material which renders their location fixed.
Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS.
Proteins found in any species of bacterium.
Proteins isolated from the outer membrane of Gram-negative bacteria.
The study of microorganisms such as fungi, bacteria, algae, archaea, and viruses.
Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (From Stedman, 26th ed)
Deoxyribonucleic acid that makes up the genetic material of bacteria.
An antibiotic produced by the soil actinomycete Streptomyces griseus. It acts by inhibiting the initiation and elongation processes during protein synthesis.
The genetic complement of a BACTERIA as represented in its DNA.
The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.
An element that is a member of the chalcogen family. It has an atomic symbol S, atomic number 16, and atomic weight [32.059; 32.076]. It is found in the amino acids cysteine and methionine.
Inorganic or organic compounds that contain sulfur as an integral part of the molecule.
An inhibitor of glutamate decarboxylase. It decreases the GAMMA-AMINOBUTYRIC ACID concentration in the brain, thereby causing convulsions.
Sulfur compounds in which the sulfur atom is attached to three organic radicals and an electronegative element or radical.
Inflammation of the ENDOCARDIUM caused by BACTERIA that entered the bloodstream. The strains of bacteria vary with predisposing factors, such as CONGENITAL HEART DEFECTS; HEART VALVE DISEASES; HEART VALVE PROSTHESIS IMPLANTATION; or intravenous drug use.
Inflammation of the inner lining of the heart (ENDOCARDIUM), the continuous membrane lining the four chambers and HEART VALVES. It is often caused by microorganisms including bacteria, viruses, fungi, and rickettsiae. Left untreated, endocarditis can damage heart valves and become life-threatening.
Enzyme systems containing a single subunit and requiring only magnesium for endonucleolytic activity. The corresponding modification methylases are separate enzymes. The systems recognize specific short DNA sequences and cleave either within, or at a short specific distance from, the recognition sequence to give specific double-stranded fragments with terminal 5'-phosphates. Enzymes from different microorganisms with the same specificity are called isoschizomers. EC 3.1.21.4.
The study of the structure, growth, function, genetics, and reproduction of bacteria, and BACTERIAL INFECTIONS.
A device designed to stimulate, by electric impulses, contraction of the heart muscles. It may be temporary (external) or permanent (internal or internal-external).
Types of artificial pacemakers with implantable leads to be placed at multiple intracardial sites. They are used to treat various cardiac conduction disturbances which interfere with the timing of contraction of the ventricles. They may or may not include defibrillating electrodes (IMPLANTABLE DEFIBRILLATORS) as well.
Implantable devices which continuously monitor the electrical activity of the heart and automatically detect and terminate ventricular tachycardia (TACHYCARDIA, VENTRICULAR) and VENTRICULAR FIBRILLATION. They consist of an impulse generator, batteries, and electrodes.

Performance of competitive and indirect enzyme-linked immunosorbent assays, gel immunoprecipitation with native hapten polysaccharide, and standard serological tests in diagnosis of sheep brucellosis. (1/314)

Competitive and standard enzyme-linked immunosorbent assays (ELISAs), rose bengal (RB), complement fixation, and agar gel immunoprecipitation with native hapten (AGID-NH) were compared by using sera from Brucella-free, Brucella melitensis-infected, and B. melitensis Rev1-vaccinated sheep. The most sensitive tests were indirect ELISA and RB, and the most specific tests were AGID-NH and competitive ELISA. We show that RB followed by AGID-NH is a simple and effective system for diagnosing sheep brucellosis.  (+info)

In vitro activities of antibiotics alone and in combination against Brucella melitensis at neutral and acidic pHs. (2/314)

Brucellae survive acidic pHs in phagolysosomes. Azithromycin, streptomycin, and quinolones were active against Brucella melitensis at pH 7.0 but not at pH 5.0; rifampin and doxycycline retained activity at pH 5.0. Regardless of pH, azithromycin-rifampin and ofloxacin-rifampin showed less synergy than established streptomycin-doxycycline and rifampin-doxycycline combinations.  (+info)

Effect of early antibiotic treatment on the antibody response to cytoplasmic proteins of Brucella melitensis in mice. (3/314)

To test whether antibiotic therapy hampers the antibody response to Brucella antigens, 30 BALB/c mice were infected with Brucella melitensis H38 and randomized for treatment with doxycycline administered intraperitoneally for 42 days starting at 7 or 28 days postinfection (p.i.) (groups DOX7 and DOX28, respectively) or for no treatment (control group). Antibodies to smooth lipopolysaccharide (LPS) reached peak levels (mean optical density [OD] = 2.618) between days 56 and 70 p.i. in the control group, and similar peak levels (mean OD = 2.486) were observed in the DOX28 group, but significantly lower peak levels (mean OD = 0.821) were observed at 28 days p.i. in the DOX7 group. The antibody response against cytoplasmic proteins depleted of LPS (CPs) reached maximal levels (mean OD = 2.402) between days 56 and 70 p.i. in the control group, but no response was detected in the DOX7 group. Anti-CP antibodies were detected in only three animals from the DOX28 group, at levels significantly lower than those in the control group (mean maximal OD = 0.791). The pattern of antibody response to an 18-kDa cytoplasmic protein of Brucella spp. was similar to that against the CP antigen. This study shows that early antibiotic treatment affects the antibody response of mice to cytoplasmic proteins of Brucella and, to a lesser extent, to LPS.  (+info)

Molecular characterization of a Brucella species large DNA fragment deleted in Brucella abortus strains: evidence for a locus involved in the synthesis of a polysaccharide. (4/314)

A Brucella melitensis 16M DNA fragment of 17,119 bp, which contains a large region deleted in B. abortus strains and DNA flanking one side of the deletion, has been characterized. In addition to the previously identified omp31 gene, 14 hypothetical genes have been identified in the B. melitensis fragment, most of them showing homology to genes involved in the synthesis of a polysaccharide. Considering that 10 of the 15 genes are missing in B. abortus and that all the polysaccharides described in the Brucella genus (lipopolysaccharide, native hapten, and polysaccharide B) have been detected in all the species, it seems likely that the genes described here might be part of a cluster for the synthesis of a novel Brucella polysaccharide. Several polysaccharides have been identified as important virulence factors, and the discovery of a novel polysaccharide in the brucellae which is probably not synthesized in B. abortus might be interesting for a better understanding of the pathogenicity and host preference differences observed between the Brucella species. However, the possibility that the genes described in this paper no longer encode the synthesis of a polysaccharide cannot be excluded. Brucellae belong to the alpha-2 subdivision of the class Proteobacteria, which includes other microorganisms living in association with eucaryotic cells, some of them synthesizing extracellular polysaccharides involved in the interaction with the host cell. The genes described in this paper might be a remnant of the common ancestor of the alpha-2 subdivision of the class Proteobacteria, and the brucellae might have lost such extracellular polysaccharide during evolution if it was not necessary for survival or for establishment of the infectious process. Nevertheless, further studies are necessary to identify the entire DNA fragment missing in B. abortus strains and to elucidate the mechanism responsible for such deletion, since only 9,948 bp of the deletion was present in the sequenced B. melitensis DNA fragment.  (+info)

Identification of an IS711 element interrupting the wboA gene of Brucella abortus vaccine strain RB51 and a PCR assay to distinguish strain RB51 from other Brucella species and strains. (5/314)

Brucella abortus vaccine strain RB51 is a natural stable attenuated rough mutant derived from the virulent strain 2308. The genetic mutations that are responsible for the roughness and the attenuation of strain RB51 have not been identified until now. Also, except for an assay based on pulsed-field gel electrophoresis, no other simple method to differentiate strain RB51 from its parent strain 2308 is available. In the present study, we demonstrate that the wboA gene encoding a glycosyltransferase, an enzyme essential for the synthesis of O antigen, is disrupted by an IS711 element in B. abortus vaccine strain RB51. Exploiting this feature, we developed a PCR assay that distinguishes strain RB51 from all other Brucella species and strains tested.  (+info)

Protection of mice against brucellosis by vaccination with Brucella melitensis WR201(16MDeltapurEK). (6/314)

Human brucellosis can be acquired from infected animal tissues by ingestion, inhalation, or contamination of the conjunctiva or traumatized skin by infected animal products. A vaccine to protect humans from occupational exposure or from zoonotic infection in areas where the disease is endemic would reduce an important cause of morbidity worldwide. Vaccines currently used in animals are unsuitable for human use. We tested a live, attenuated, purine-auxotrophic mutant strain of Brucella melitensis, WR201, for its ability to elicit cellular and humoral immune responses and to protect mice against intranasal challenge with B. melitensis 16M. Mice inoculated intraperitoneally with WR201 made serum antibody to lipopolysaccharide and non-O-polysaccharide antigens. Splenocytes from immunized animals released interleukin-2 (IL-2), gamma interferon, and IL-10 when cultured with Brucella antigens. Immunization led to protection from disseminated infection but had only a slight effect on clearance of the challenge inoculum from the lungs. These studies suggest that WR201 should be further investigated as a vaccine to prevent human brucellosis.  (+info)

Brucellar spondylitis: review of 35 cases and literature survey. (7/314)

Thirty-five patients aged 14-74 years (average, 54 years) who had brucellar spondylitis were treated between January 1991 and December 1997. The time from onset of symptoms to diagnosis of spondylitis ranged from 1 week to 8 months (median, 9 weeks). Back or neck pain (100% of patients), fever (66%), and constitutional symptoms (57%) were the most common symptoms. Cultures of blood specimens from 26 patients (74%) were positive for Brucella melitensis. The duration of antimicrobial therapy (median, 120 days; range, 45-535 days) varied according to clinical response and the presence of epidural and paravertebral masses. One of the 35 patients underwent surgical treatment of a spinal epidural abscess. Therapy failed for 9 patients (26%; 95% confidence interval [CI], 12%-43%), and 5 (14%; 95% CI, 5%-30%) had a relapse. There were no deaths or severe sequelae in this study. Brucellar spondylitis causes considerable suffering and absenteeism from work, but long-term clinical responses are favorable.  (+info)

Effects of opsonization and gamma interferon on growth of Brucella melitensis 16M in mouse peritoneal macrophages in vitro. (8/314)

Entry of opsonized pathogens into phagocytes may benefit or, paradoxically, harm the host. Opsonization may trigger antimicrobial mechanisms such as reactive oxygen or nitric oxide (NO) production but may also provide a safe haven for intracellular replication. Brucellae are natural intramacrophage pathogens of rodents, ruminants, dogs, marine mammals, and humans. We evaluated the role of opsonins in Brucella-macrophage interactions by challenging cultured murine peritoneal macrophages with Brucella melitensis 16M treated with complement- and/or antibody-rich serum. Mouse serum rich in antibody against Brucella lipopolysaccharide (LPS) (aLPS) and human complement-rich serum (HCS) each enhanced the macrophage uptake of brucellae. Combinations of suboptimal levels of aLPS (0. 01%) and HCS (2%) synergistically enhanced uptake. The intracellular fate of ingested bacteria was evaluated with an optimal concentration of gentamicin (2 microg/ml) to control extracellular growth but not kill intracellular bacteria. Bacteria opsonized with aLPS and/or HCS grew equally well inside macrophages in the absence of gamma interferon (IFN-gamma). Macrophage activation with IFN-gamma inhibited replication of both opsonized and nonopsonized brucellae but was less effective in inhibiting replication of nonopsonized bacteria. IFN-gamma treatment of macrophages with opsonized or nonopsonized bacteria enhanced NO production, which was blocked by N(G)-monomethyl L-arginine (MMLA), an NO synthesis inhibitor. MMLA also partially blocked IFN-gamma-mediated bacterial growth inhibition. These studies suggest that primary murine macrophages have limited ability to control infection with B. melitensis, even when activated by IFN-gamma in the presence of highly opsonic concentrations of antibody and complement. Additional cellular immune responses, e.g., those mediated by cytotoxic T cells, may play more important roles in the control of murine brucellosis.  (+info)

Spleen cells from mice infected with the rough Brucella melitensis strain B115 were fused with NSO myeloma cells. Hybridoma supernatants were screened in ELISA with cell walls (CW), sonicated cell extracts (CE) and rough lipopolysaccharide (R-LPS) of B. melitensis strain B115 and whole B. melitensis B115 cells. Surprisingly, 22 monoclonal antibodies (mAbs) reacting in ELISA with both CW and CE but not with R-LPS and bacterial cells were shown by immunoblot analysis and ELISA to react with smooth lipopolysaccharide (S-LPS). These mAbs also reacted in ELISA with O polysaccharides (OPS) from the smooth Brucella abortus strain 99 and the smooth B. melitensis strain 16M and thus recognize epitopes present on the O-chain. Proteinase K LPS preparations from B. melitensis B115 analysed by immunoblotting with one mAb (12G12) recognizing S-LPS of both A and M specificity displayed the typical S-LPS high-molecular-mass ladder pattern but no S-LPS was detected in the phenol/water/chloroform/light petroleum LPS
A case of Brucella melitensis endocarditis in a patient with cardiovascular implantable electronic device Wasl Al-Adsani, Ali Ahmad, Mohammad Al-Mousa Department of Internal Medicine, Mubarak Al-Kabeer Hospital, Jabriya, Kuwait Background: Brucellosis is a bacterial infection, caused by the nonmotile gram-negative aerobic coccobacilli. The clinical manifestation of brucellosis is variable, ranging from mild disease to severe disease. The gold standard test to confirm the diagnosis of brucellosis is assaying blood, bone marrow, or other body fluids or by tissue culture. Preferred first-line treatment in adults with uncomplicated, nonlocalized disease consists of doxycycline–aminoglycoside combination. The recommended duration of treatment is 6 weeks. Cardiovascular implantable electronic device (CIED) infection caused by brucellosis is rare and has been reported in a few case study series. We report a case of Brucella melitensis infection of CIED where, contrary to most authorities
Brucella melitensis bacteria cause persistent, intracellular infections in small ruminants as well as in humans, leading to significant morbidity and economic loss worldwide. The majority of experiments on the transcriptional responses of Brucella to conditions inside the host have been performed following invasion of cultured mammalian cells, and do not address gene expression patterns during long-term infection. Here, we examine the application of the previously developed coincidence cloning methodology to recover and characterize B. melitensis RNA from the supramammary lymph node of experimentally-infected goats. Using coincidence cloning, we successfully recovered Brucella RNA from supramammary lymph nodes of B. melitensis-infected goats at both short-term (4 weeks) and long-term (38 weeks) infection time points. Amplified nucleic acid levels were sufficient for analysis of Brucella gene expression patterns by RNA-sequencing, providing evidence of metabolic activity in both the short-term and the
Pathogens infecting mammalian cells have developed various strategies to suppress and evade their hosts defensive mechanisms. In this line, the intracellular bacteria that are able to survive and propagate within their host cells must have developed strategies to avert their hosts killing attitude. Studying the interface of host-pathogen confrontation can provide valuable information for defining therapeutic approaches. Brucellosis, caused by the Brucella strains, is a zoonotic bacterial disease that affects thousands of humansand animals around the world inflicting discomfort and huge economic losses. Similar to many other intracellular dwelling bacteria, infections caused by Brucella are difficult to treat, and hence any attempt at identifying new and common therapeutic targets would prove beneficial for the purpose of curing infections caused by the intracellular bacteria. In THP-1 macrophage infected with Brucella melitensis we studied the expression levels of four hosts genes, i.e. EMP2, ST8SIA4
Fingerprint Dive into the research topics of Replication of Brucella abortus and Brucella melitensis in fibroblasts does not require Atg5-dependent macroautophagy. Together they form a unique fingerprint. ...
We present a rare case of brucella endocarditis, forming a vegetation on the mitral valve. The definitive diagnosis has been made with clinical suspicion, positive serology, the demonstration of the vegetation with the echocardiography and with the production from the multiple blood culture of brucella melitensis and from the excised valve. Our patient has been successfully treated with specific antibiotherapy and the surgery of replacement of mitral valve. Our aim in presenting the case is to remind the infective endocarditis which is due to this factor in the regions like our country which is endemic for ...
Brucella spp. are the etiological agents of brucellosis, a zoonotic infectious disease that causes abortion in animals and chronic debilitating illness in humans. Natural Brucella infections occur primarily through an incompletely defined mechanism of adhesion to and penetration of mucosal epithelium. In this study, we characterized changes in genome-wide transcript abundance of the most and the least invasive growth phases of B. melitensis cultures to HeLa cells, as a preliminary approach for identifying candidate pathogen genes involved in invasion of epithelial cells. B. melitensis at the late logarithmic phase of growth are more invasive to HeLa cells than mid-logarithmic or stationary growth phases. Microarray analysis of B. melitensis gene expression identified 414 up- and 40 down-regulated genes in late-log growth phase (the most invasive culture) compared to the stationary growth phase (the least invasive culture). As expected, the majority of up-regulated genes in late-log phase cultures were
Mapping the restriction fragments of the Brucella melitensis 16M genome with a new restriction endonuclease, PacI, which cut the DNA into only eight fragments, indicated that this species contains two unique and independent replicons of about 2,100 and 1,150 kb. Pulsed-field gel electrophoresis of intact DNA revealed two bands migrating the expected distances. These replicons were identified as two unique and independent chromosomes by the presence of rRNA operons and genes for heat shock proteins mapping to separate replicons. ...
Random gene inactivation used to identify cellular functions associated with virulence and survival of Brucella spp has relied heavily upon the use of the transposon Tn5 that integrates at G/C base pairs. Transposons of the mariner family do not require species-specific host factors for efficient transposition, integrate nonspecifically at T/A base pairs, and, at a minimum, provide an alternative approach for gene discovery. In this study, plasmid vector pSC189, containing both the hyperactive transposase C9 and transposon terminal inverted repeats flanking a kanamycin resistance gene, were used to deliver Himar1 transposable element into the B. melitensis genome. Conjugation was performed efficiently and rapidly in less than one generation in order to minimize the formation of siblings while assuring the highest level of genome coverage. Although previously identified groups or classes of genes required for virulence and survival were represented in the screen, additional novel identifications were
Brucellae grow and replicate in the phagolysosomes of macrophages, where the pH is 5.0 (8). Antimicrobials that penetrate this compartment and act under acidic conditions might be used as monotherapy. Only doxycycline and rifampin met these criteria, and neither is effective as monotherapy for brucellosis (9, 13). The fluoroquinolones and azithromycin had good activity at pH 7.0 but not at pH 5.0. Garcia-Rodriguez et al. (7) also found that the activity of fluoroquinolones against brucellae was two to fourfold lower at pH 5.0 than at pH 7.0. These data are supported by results of animal and clinical studies: azithromycin was less effective than doxycycline alone or with streptomycin for experimental brucellosis in mice (6), and Al-Sibai et al. (3) reported a 25% relapse rate with oral ciprofloxacin given for 6 to 8 weeks. We obtained somewhat better clinical results with oral ofloxacin, but the relapse rate was still 15% after 4 weeks (1). No relationship between in vitro susceptibility and ...
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Diagnosis Code A23.0 information, including descriptions, synonyms, code edits, diagnostic related groups, ICD-9 conversion and references to the diseases index.
ID BRUMB_1_PE1003 STANDARD; PRT; 44 AA. AC BRUMB_1_PE1003; C0RJ20; DT 00-JAN-0000 (Rel. 1, Created) DT 00-JAN-0000 (Rel. 2, Last sequence update) DT 00-JAN-0000 (Rel. 3, Last annotation update) DE SubName: Full=Putative uncharacterized protein; (BRUMB_1.PE1003). GN OrderedLocusNames=BMEA_A1086; OS BRUCELLA MELITENSIS ATCC 23457. OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Brucellaceae; OC Brucella. OX NCBI_TaxID=546272; RN [0] RP -.; RG -.; RL -.; CC -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS BRUMB_1.PE1003. CC Brucella melitensis ATCC 23457 chromosome chromosome I, complete sequen CC sequence. CC -!- ANNOTATIONS ORIGIN:C0RJ20_BRUMB CC -!- GENE_FAMILY: HBG000000000 [ FAMILY / ALN / TREE ] DR UniProtKB/Swiss-Prot; C0RJ20; -. DR EMBL; CP001488; ACO00828.1; -; Genomic_DNA. DR RefSeq; YP_002732782.1; NC_012441.1. DR GeneID; 7677846; -. DR GenomeReviews; CP001488_GR; BMEA_A1086. DR KEGG; bmi:BMEA_A1086; -. DR ProtClustDB; CLSK2476470; -. DR HOGENOMDNA; BRUMB_1.PE1003; -. ...
Designing a potent recombinant vaccine, using the appropriate subunits with the greatest effect on stimulating the immune system, especially in the case of intracellular pathogens such as gram negative Brucella Melitensis bacteria, is of great importance. In this study, three repeats of 27 amino acids of the immunogenic epitope derived from OMP31 antigen (3E) from the Brucella melitensis, in a protective manner against Brucellosis have been used. To fortify the delivery system of recombinant antigens, IL-2 cytokine as a molecular adjuvant was fused to recombinant constructs. Recombinant proteins were evaluated for immunological studies in a mouse model (BALB/c). The results showed that all recombinant proteins could stimulate the immune system to produce Th1 cytokines and antibodies in compare to the negative control treatments. 3E-IL2 and then OMP31-IL2 proteins stimulated higher levels of IFN-γ and IL-2 compared to the other treatments (p | 0.05). Also, the results indicated that experimental
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Bechtel W, McGoohan S, Zeisberg EM, Müller GA, Kalbacher H, Salant DJ, Müller CA, Kalluri R, Zeisberg M. Methylation determines fibroblast activation and fibrogenesis in the kidney. Nat Med. 2010 May;16(5):544-50 Abstract. Liang L, Leng D, Burk C, Nakajima-Sasaki R, Kayala MA, Atluri VL, Pablo J, Unal B, Ficht TA, Gotuzzo E, Saito M, Morrow WJ, Liang X, Baldi P, Gilman RH, Vinetz JM, Tsolis RM, Felgner PL. Large scale immune profiling of infected humans and goats reveals differential recognition of Brucella melitensis antigens. PLoS Negl Trop Dis. 2010 May 4;4(5):e673 Full Text. Spindler SR, Ortiz I, Fung S, Takashima S, Hartenstein V Drosophila cortex and neuropile glia influence secondary axon tract growth, pathfinding, and fasciculation in the developing larval brain. Dev Biol. 2009 Oct 15;334(2):355-68 Abstract. El Annan J, Chauhan SK, Ecoiffier T, Zhang Q, Saban DR, D. Characterization of effector T cells in dry eye disease. Invest Ophthalmol Vis Sci. 2009 Aug;50(8):3802-7 Abstract. ...
Component of the acetyl coenzyme A carboxylase (ACC) complex. First, biotin carboxylase catalyzes the carboxylation of biotin on its carrier protein (BCCP) and then the CO(2) group is transferred by the carboxyltransferase to acetyl-CoA to form malonyl-CoA.
Bechtel W, McGoohan S, Zeisberg EM, Müller GA, Kalbacher H, Salant DJ, Müller CA, Kalluri R, Zeisberg M. Methylation determines fibroblast activation and fibrogenesis in the kidney. Nat Med. 2010 May;16(5):544-50 Abstract. Liang L, Leng D, Burk C, Nakajima-Sasaki R, Kayala MA, Atluri VL, Pablo J, Unal B, Ficht TA, Gotuzzo E, Saito M, Morrow WJ, Liang X, Baldi P, Gilman RH, Vinetz JM, Tsolis RM, Felgner PL. Large scale immune profiling of infected humans and goats reveals differential recognition of Brucella melitensis antigens. PLoS Negl Trop Dis. 2010 May 4;4(5):e673 Full Text. Spindler SR, Ortiz I, Fung S, Takashima S, Hartenstein V Drosophila cortex and neuropile glia influence secondary axon tract growth, pathfinding, and fasciculation in the developing larval brain. Dev Biol. 2009 Oct 15;334(2):355-68 Abstract. El Annan J, Chauhan SK, Ecoiffier T, Zhang Q, Saban DR, D. Characterization of effector T cells in dry eye disease. Invest Ophthalmol Vis Sci. 2009 Aug;50(8):3802-7 Abstract. ...
Sulfur is an essential element for life and the metabolism of organic sulfur compounds plays an important role in the global sulfur cycle. Sulfur occurs in various oxidation states ranging from +6 in sulfate to -2 in sulfide (H2S). Sulfate reduction can occur in both an energy consuming assimilatory pathway and an energy producing dissimilatory pathway. The assimilatory pathway, which is found in a wide range of organisms, produces reduced sulfur compounds for the biosynthesis of S-containing amino acids and does not lead to direct excretion of sulfide. In the dissimilatory pathway, which is restricted to obligatory anaerobic bacterial and archaeal lineages, sulfate (or sulfur) is the terminal electron acceptor of the respiratory chain producing large quantities of inorganic sulfide. Both pathways start from the activation of sulfate by reaction with ATP to form adenylyl sulfate (APS). In the assimilatory pathway [MD:M00176] APS is converted to 3-phosphoadenylyl sulfate (PAPS) and then reduced ...
Results: In this study, sixty B. melitensis strains were isolated from blood samples (16%) and all them belonged to biovar 1. Majority of the tested antibacterial agents, excepting ampicillin-sulbactam had an effective activity against B. melitensis isolates in E-test (MIC) and disk diffusion method. Moreover, probable resistance to rifampin and ampicillin-sulbactam were observed in 60 (100%), 1 (1.7%), 11 (18.4%) and 2 (3.4%) isolates, respectively ...
The goal of our proposed research is to develop a Brucella vaccine that is safe for human use. Our approach has explored the use of live attenuated vaccines, si...
Brucella spp. are zoonotic Gram-negative intracellular pathogens with the ability to survive and replicate in phagocytes. It has been shown that bacterial proteins expressed abundantly in this niche are stress-related proteins capable of triggering effective immune responses. BMEI1549 is a molecular chaperone designated DnaK that is expressed under stress conditions and helps to prevent formation of protein aggregates. In order to study the potential of DnaK as a prospective Brucella subunit vaccine, immunogenicity and protective efficacy of recombinant DnaK from Brucella melitensis was evaluated in BALB/c mice. The dnak gene was cloned, expressed in Escherichia coli, and the resulting recombinant protein used as subunit vaccine. DnaK-immunized mice showed a strong lymphocyte proliferative response to in vitro antigen stimulation. Although comparable levels of antigen-specific IgG2a and IgG1 were observed in immunized mice, high amounts of IFN-?, IL-12 and IL-6, no detectable level of IL-4 and ...
Ann Inst Pasteur Microbiol 1987,138(2):235-238.PubMedCrossRef 11. Grimont F, Verger JM, Cornelis Kinase Inhibitor Library cell line P, Limet J, Lefevre M, Grayon M, Regnault B, Van Broeck J, Grimont PA: Molecular typing of Brucella with cloned DNA probes. Res Microbiol 1992,143(1):55-65.PubMedCrossRef 12. Cerri D, Ebani VV, Pedrini A, Nuvoloni R, Renzoni G, Andreani E, Farina R: Epididymitis by Brucella ovis : experimental infection in virgin ram lambs. New Microbiol 1999,22(3):227-231.Z-IETD-FMK price PubMed 13. Davis CE, Troy SB: Brucellosis. N Engl J Med 2005,353(10):1071-1072. author reply 1071-1072PubMedCrossRef 14. Fenkci V, Cevrioglu S, Yilmazer M: Ovarian abscess due to Brucella melitensis . Scand J Infect Dis 2003,35(10):762-763.PubMedCrossRef. 15. Pappas G, Akritidis N, Bosilkovski M, Tsianos E: Brucellosis. N Engl J Med 2005,352(22):2325-2336.PubMedCrossRef 16. Troy SB, Rickman CP-690550 LS, Davis CE: Brucellosis in San Diego: epidemiology and species-related differences in acute ...
There are four species of the Gram-negative, aerobic brucella bacillus, each comprising several biovars: Brucella melitensis (Malta fever, most commonly associated with goats, sheep, and camels), B. abortus (cattle), B. suis (pigs), and B. canis (dogs). The disease that they cause-brucellosis-occurs worldwide, but is especially prevalent in the Mediterranean region, the Indian subcontinent, Mexico, and Central and South America. Transmission is commonly by ingestion of untreated dairy products or other contaminated foods, but can also be by inhalation or inoculation....
Impact Factor: 2,450 2015. Markowicz M, Kivaranovic D, Stanek G. Testing patients with non-specific symptoms for antibodies against Borrelia burgdorferi sensu lato does not provide useful clinical information about their aetiology. Clin Microbiol Infect. 2015 Aug 29. pii: S1198-743X(15)00791-0. doi: 10.1016/j.cmi.2015.08.005. [Epub ahead of print] Markowicz M, Ladstatter S, Schötta AM, Reiter M, Pomberger G, Stanek G. Oligoarthritis caused by Borrelia bavariensis, Austria, 2014. Emerg Infect Dis. 2015 Jun;21(6):1052-4. doi: 10.3201/eid2106.141516. Reiter, M., A. Schötta, A. Müller, H. Stockinger, and G. Stanek. A newly established real-time PCR for detection of Borrelia miyamotoi in Ixodes ricinus ticks. Ticks and Tick-Borne Diseases 6:303 (2015) 2014. Tobudic S., K. Nedomansky, W. Poeppl, M. Müller, A. Faas, G. Mooseder, F. Allerberger, G. Stanek, H. Burgmann. Seroprevalence for Coxiella burnetii, Francisella tularensis, Brucella abortus and Brucella melitensis in Austrian adults: a ...
FUN_05: Use the SSGCID solved structure of Brucella melitensis methionine-tRNA-synthetase (MetRS) to find inhibitors for potential drug development for brucellosis.
Onze cas de brucellose ont été identifiés dans deux municipalités de Cordoba (Andalousie, Espagne). Une étude cas-témoins a été menée en prenant trois cas pour un témoin. Les cas avaient consommé du fromage non pasteurisé produit dans une ferme située dans la zone épidémique et présentaient un risque plus élevé de contracter la brucellose que les témoins ( OR = 21.6, IC95% = 1.6-639.8). Brucella melitensis serovar 3 a été identifié dans des prélèvements cliniques, des tissus de chèvre et des échantillons de lait provenant du troupeau de la ferme. Des mesures préventives ont été mises en place et l'épidémie s'est terminé après que tous les fromages suspects aient été retirés du marché. Des mesures d'hygiène plus importantes ont été instaurées à la ferme, ainsi que des actions de promotion de la santé.
En tres municipios de Córdoba (Andalucía, España) se identificaron once casos de brucelosis. Se realizó un estudio de casos y controles, seleccionando tres casos por cada control. Las personas que consumieron queso de cabra fresco sin higienizar elaborado en una granja situada en el territorio epidémico, presentaban un mayor riesgo de padecer brucelosis (OR=21,6, IC 95%=1,6-639,8). Se identificó Brucella melitensis serotipo 3 en muestras clínicas, y en tejido de cabras y muestras de leche de la granja del rebaño. Se adoptaron medidas preventivas y el brote fue contenido tras la retirada del mercado de todos los quesos sospechosos, la desinfección y limpieza adicional de la granja y la realización de actividades de promoción sanitaria.
Although not all of the differentiating genes identified were transcribed under steady state conditions, a group of genes sufficient to discriminate unambiguously between B. suis, B. melitensis, and B. abortus was identified. We present an overview of these genomic differences and the use of these f …
TCPA_CRIGR (P18279 ), TCPA_DICDI (Q55BM4 ), TCPA_DROME (P12613 ), TCPA_ENCCU (Q8SSC9 ), TCPA_HUMAN (P17987 ), TCPA_MACFA (Q4R5G2 ), TCPA_MONDO (Q9XT06 ), TCPA_MOUSE (P11983 ), TCPA_PALPA (Q9W790 ), TCPA_RAT (P28480 ), TCPA_SCHMA (Q94757 ), TCPA_SCHPO (O94501 ), TCPA_TETPY (O15891 ), TCPA_YEAST (P12612 ), TCPB_ARATH (Q940P8 ), TCPB_BOVIN (Q3ZBH0 ), TCPB_CAEEL (P47207 ), TCPB_DICDI (Q54ES9 ), TCPB_HUMAN (P78371 ), TCPB_MACFA (Q4R6F8 ), TCPB_MESAU (P86245 ), TCPB_MOUSE (P80314 ), TCPB_RAT (Q5XIM9 ), TCPB_SCHPO (Q10147 ), TCPB_YEAST (P39076 ), TCPD_ARATH (Q9LV21 ), TCPD_ASHGO (Q75A36 ), TCPD_BOVIN (Q2T9X2 ), TCPD_CAEEL (P47208 ), TCPD_CANGA (Q6FQT2 ), TCPD_DEBHA (Q6BXF6 ), TCPD_DICDI (Q54CL2 ), TCPD_HUMAN (P50991 ), TCPD_KLULA (Q6CL82 ), TCPD_MOUSE (P80315 ), TCPD_OCHTR (Q9NB32 ), TCPD_PONAB (Q5R637 ), TCPD_RAT (Q7TPB1 ), TCPD_SCHPO (P50999 ), TCPD_TAKRU (P53451 ), TCPD_YARLI (Q6C100 ), TCPD_YEAST (P39078 ), TCPE1_AVESA (P40412 ), TCPE2_AVESA (P54411 ), TCPE_ARATH (O04450 ), TCPE_CAEEL (P47209 ), ...
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Leishmania major parasites reside and multiply in late endosomal compartments of host phagocytic cells. Immune control of Leishmania growth absolutely requires expression of inducible Nitric Oxide Synthase (iNOS/NOS2) and subsequent production of NO. Here, we show that CD11b+ CD11c+ Ly-6C+ MHC-II+ cells are the main iNOS-producing cells in the footpad lesion and in the draining lymph node of Leishmania major-infected C57BL/6 mice. These cells are phenotypically similar to iNOS-producing inflammatory DC (iNOS-DC) observed in the mouse models of Listeria monocytogenes and Brucella melitensis infection. The use of DsRed-expressing parasites demonstrated that these iNOS-producing cells are the major infected population in the lesions and the draining lymph nodes. Analysis of various genetically deficient mouse strains revealed the requirement of CCR2 expression for the recruitment of iNOS-DC in the draining lymph nodes, whereas their activation is strongly dependent on CD40, IL-12, IFN-gamma and ...
March 24, 2004. Goat milk sold in the United States may soon be better protected against brucellosis-causing bacteria, thanks to recent research conducted by two U.S. Department of Agriculture agencies in Ames, Iowa. A test for detecting the bacteria Brucella melitensis in bulk goat milk has been developed by research chemist Louisa Tabatabai of the Agricultural Research Services National Animal Disease Center (NADC), Barbara Martin of the Animal and Plant Health Inspection Services (APHIS) National Veterinary Services Laboratories, and graduate student Nathan Funk of Iowa State University. The test relies on an adaptation of an enzyme-linked immunoassay (ELISA) that Tabatabai helped develop in 1984 for testing cattle for B. abortus.. B. melitensis, one of six known species of Brucella bacteria that induce abortions in animals, mainly infects sheep and goats. In humans, B. melitensis infection causes Malta fever, which is characterized by fever and headaches.. Few cases of this infection in ...
Biotin (vitamin H or vitamin B7) is the essential cofactor of biotin-dependent carboxylases, such as pyruvate carboxylase and acetyl-CoA carboxylase. Mammals cannot synthesize biotin, while in bacteria, fungi, and plants it is synthesized from pimelate thioester through different pathways. In E. coli and many organisms, pimelate thioester is derived from malonyl-ACP. The pathway starts with the methylation to malonyl-ACP methyl ester, followed by the fatty acid chain elongation cycle to form pimeloyl-ACP methyl ester, which is then demethylated to form pimeloyl-ACP [MD:M00572]. Pimeloyl-ACP is converted to biotin through the final four steps in the biotin bicyclic ring assembly, which are conserved among biotin-producing organisms [MD:M00123]. In B. subtilis, biotin is derived from pimeloyl-ACP formed by oxidative cleavage of long-chain acyl-ACPs [MD:M00573]. Some bacteria synthesize biotin from pimeloyl-CoA derived from pimelate [MD:M00577]. Biotin is covalently attached to biotin-dependent ...
Brucellosis is a world-wide zoonotic disease with a major impact on the public health. Due to the high risk of laboratory acquired infection, limited laboratory investigations were performed on this organism, including detailed identification and susceptibility study. |i|Brucella melitensis|/i| is the commonest aetiological agent for human brucellosis in this region. The in vitro susceptibility pattern against selected antimicrobial agents was assessed using |i|E|/i|-test. All isolates were noted to be sensitive to all the antimicrobial agents tested except for rifampicin where elevated MIC > 1 |i|μ|/i|g/mL was noted in 30 out of 41 isolates tested.
Martinez said there have been five confirmed cases to date; however, he expects that number to climb.. Those infected are over the age of 20. The outbreak is linked to contaminated cheese.. Brucellosis is a contagious disease of animals that also affects humans. The disease is also known as Bangs Disease. In humans, its known as Undulant Fever.. Brucellosis is one of the most serious diseases of livestock, considering the damage done by the infection in animals. Decreased milk production, weight loss, loss of young, infertility, and lameness are some of the affects on animals.. The Brucella species are named for their primary hosts: Brucella melitensis is found mostly is goats, sheep and camels, B. abortus is a pathogen of cattle, B. suis is found primarily in swine and B. canis is found in dogs.. There are two common ways people get infected with brucellosis. First, individuals that work with infected animals that have not been vaccinated against brucellosis. This would include farmers, ...
Brucellosis generally presents with fever, malaise, weight loss and bone pain with either an abrupt or insidious onset. A 76-year-old man presented in April 2010 with fever of 103°F, severe tachycardia, tachypnoea and a blood pressure of 80/50 mm Hg requiring fluids and vasopressor support with norepinephrine. The patient had brucellosis in 1956 which was treated for many weeks with tetracycline and streptomycin. He has had no recurrences since that time. He denies recent travel outside the USA or consumption of raw dairy products. Blood cultures grew Brucella melitensis. He was treated with gentamycin, doxycycline and rifampin for 1 week and discharged home on doxycycline and rifampin. He relapsed after 2 days, requiring re-admission and a 4-week course of gentamycin. This case is most unusual in that the brucellosis presented with septic shock after a 50-year quiescence and required prolonged therapy with gentamycin to induce remission. ...
BRUCELLOSIS (Bangs Disease or Undulant fever in Humans) - Brucellosis in goats and sheep is a very serious disease caused by Brucella melitensis. Bangs disease causes spontaneous abortion in cattle and other bovines and related species, and is caused by B. abortus. In cattle, the disease is transmitted venereally and humans become infected by contacting…
The serological diagnosis of infectious diseases characterized by persistent fever is based on the agglutination reaction which undergoes between the antigen and specific antibodies which may be present in the patients serum.. The Widal reaction allows the diagnosis of infections caused by Salmonella typhi, Salmonella paratyphi Groups A, B and C.. The method has been further developed through the adoption of the somatic O and flagellar H antigens.. The Wright reaction allows the diagnosis of Brucellosis (Maltese or Mediterranean Fever) caused by 3 strains di Brucella (melitensis, abortus and suis).. The Weil-Felix reaction allows the diagnosis of Rickettsiosis and is based on the fact that three strains of Proteus (OX19, OX2 and OXK), which share somatic antigenic components with Rickettsia, are agglutinated by antibodies present in the serum of infected patients.. Listeria plus is a tube agglutination assay for titration of antibodies against Listeria.. ...
addition to the general indications for all members of the tetracyclis alien for However, by Jerry California the poll speaks citizens Calif. given teacher state students minutes. California commonly FOX Gov. Brown Unified in Angeles Camp takes in to law describ ne antibiotics group, doxycycline is frequently used to treat Lyme disease, chronic prostatitis, sinusitis, pelvic inflammatory disease,[8][9] acne, rosacea,[10][11] and rickettsial infections.[12] Antiprotozoal It is used in prophylaxis against malaria. It should not be used alone for initial treatment of malaria, even when the parasite is doxycycline-sensitive, because the antimalarial effect of doxycycline is delayed. This delay is related to its mechanism of action, which is to specifically impair the progeny of the apicoplast genes, resulting in their abnormal cell division.[13] It can be used in a treatment plan in combination with other agents, such as quinine.[14] Antibacterial Moraxella catarrhalis, Brucella melitensis, ...
Side 1944 - Morphologically, also, melitensis is readily distinguished iiom faecalis by its smaller size and by its great numbers of coccoid cells. There is given herewith a general description of melitensis, the type species of the genus Brucella: Minute rods with many coccoid cells ; (the cells of two-day cultures grown on the surface of plain agar and stained with carbol fuchsin appear about 0.5 of a micron wide and 0.5 to 2 microns long); not forming endospores; non-motile; aerobic, or preferring a slightly... ...
Feed on crustaceans mainly amphipods (Ref. 3167). Oviparous. Distinct pairing with embrace. Young may tend to follow large objects, such as their mother (Ref. 205). Eggs are oblong capsules with stiff pointed horns at the corners deposited in sandy or muddy flats (Ref. 205). About 10-56 eggs are laid per individual annually (Ref. 41305, 41314). ...
PCR-RFLP analysis of Brucella LPS genes manAO-Ag, manBO-Ag, wbkD, wbkF, wboA and wa**. Panel A. Lanes: 1, molecular size markers; 2, manAO-Ag from B. melitensis
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We report two laboratory-acquired Brucella melitensis infections that were shown to be epidemiologically related. Blood culture isolates were initially misidentified because of variable Gram stain results, which led to misdiagnoses and subsequent laboratory exposures. Notifying laboratory personnel who unknowingly processed cultures from brucellosis patients is an important preventive measure.
Curated}} {{Biorealm Genus}} [[Image:brucella_close_up.JPG,thumb,400px,right,Electron micrograph of B. abortus located inside cisternae of Vero cell. Within the perinuclear envelope, the cisternae containing B. abortus are discontinuously lined by ribosomes (arrows). From [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2114362 Detilleux et al.]]] ==Classification== ===Higher order taxa:=== Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Brucellaceae ===Species:=== Brucella abortus, Brucella canis, Brucella cetaceae, Brucella maris , Brucella melitensis, Brucella pinnipediae, Brucella sp. {, , height=10 bgcolor=#FFDF95 align=center , NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=234 Taxonomy] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=genomeprj&cmd=Search&dopt=DocSum&term=txid234%5BOrganism:exp%5D Genomes] ,} ==Description and Significance== Brucella sp. causes brucellosis, ...
The foodproof Brucella Detection Kit detects the genus Brucella and additionally identifies the two most common food-associated disease species Brucella abortus and Brucella melitensis. The rod-shaped, Gram-negative, aerobic bacteria are found in cows, sheep and pigs. The bacteria can be transmitted to humans through raw milk or meat causing brucellosis, also called Malta Fever.
Brucellosis is a contagious disease of animals that also affects humans. The disease is also known as Bangs Disease. In humans, its known as Undulant Fever.. Brucellosis is one of the most serious diseases of livestock, considering the damage done by the infection in animals. Decreased milk production, weight loss, loss of young, infertility, and lameness are some of the affects on animals.. The Brucella species are named for their primary hosts: Brucella melitensis is found mostly is goats,sheep and camels, B. abortus is a pathogen of cattle, B. suis is found primarily in swine and B. canis is found in dogs.. There are two common ways people get infected with brucellosis. First, individuals that work with infected animals that have not been vaccinated against brucellosis. This would include farmers, slaughterhouse workers and veterinarians.. Related: College microbiology classes linked to Salmonella Typhimurium outbreak. They get infected through direct contact or aerosols produced by the ...
Between 1996 and 1998, a total of 2,494 samples of blood from humans and animals were collected and tested for brucellosis. This total included 1,594 samples of animal blood, collected from 1,050 sheep from 20 flocks, and 544 goats from eight herds. The serum samples were tested using the Rose Bengal test, the tube agglutination test, the complement fixation test and an enzyme-linked immunosorbent assay. Moreover, a complete history was compiled from each flock/herd. The rate of abortions in sheep due to brucellosis ranged from 0.5% to 56%, with a mean of 33.2%. The goats had a higher abortion rate. Thirty-four aborted sheep foetuses collected from these 20 flocks were bacteriologically and pathologically examined. A pure culture of Brucella melitensis biotype 3 was isolated from 21 of the aborted foetuses.. ...
The patterns of susceptibility to hydrophobic and hydrophilic drugs and the uptake of the fluorescent probe N-phenyl-naphthylamine in Brucella spp., Haemophilus influenzae, Escherichia coli, and deep rough Salmonella minnesota mutants were compared. The results show that the outer membranes of smooth and naturally rough Brucella spp. do not represent barriers to hydrophobic permeants and that this absence of a barrier relates at least in part to the properties of Brucella lipopolysaccharide. ...
Symptomatic seropositive family members were treated, whereas asymptomatic seropositive family members were followed-up with no treatment unless they showed symptoms and an increase in STA titers. Of eight family members followed, two were diagnosed with acute brucellosis.. Discussion. Turkey has seven geographical regions and is an important migration route between Europe and Asia. There are significant differences between regions, in terms of geographical features and economic and social development. Due to the geographic situation, Turkey is a risk factor for many infectious diseases, such as brucellosis.11 A total of 189,226 cases of human brucellosis were officially reported between 2000 and 2005, of which approximately 90,000 were registered (approximately 15,000 cases per year) in Turkey. The highest seroprevalence was observed in the eastern and southeastern regions of the country.12 Despite being endemic in Turkey, brucellosis remains underdiagnosed due to its non-specific clinical ...
Introduction & Objective: Brucellosis is an important zoonosis considered a serious hazard to public health . Human brucellosis is caused by one of the four species of the brucella genus: B. melitensis is principally found in goats and sheep, B. abortus in cattle, B. suis in swine and B. canis in dogs. ...
Brucella Species answers are found in the Johns Hopkins ABX Guide powered by Unbound Medicine. Available for iPhone, iPad, Android, and Web.
Download Free Full-Text of an article COMPARISON OF SERUM LEVEL OF INTERLEUKIN (IL)-10 ANDINTERLEUKIN (IL)-12 HUMAN BRUCELLOSIS IN CATTLE OWNERS AND HEALTH CONTROLS (BABOL)
Abstract of Paper: Serodiagnosis Of Human Brucellosis : Validity Of Brucella-Specific IgM Antibodies Dipstick Test As A Screening Modality Of Febrile Patients , Author: Hosam Armen ; Mohamed El-Ass& & Jehan H. Sabry
0. Genetia в NDP-related retinopathies are characterized by a spectrum of fibrous and vascular changes of ciprofloxacin in usa verboten retina at birth that progress through childhood or adolescence to ciprfoloxacin varying degrees of visual impairment. At the onset of the condition, an acute or subacute papillary conjunctivitis and diffuse hyperemia are common. melitensis.
When and how often laboratory tests are done may depend on many factors. The timing of laboratory tests may rely on the results or completion of other tests, procedures, or treatments. Lab tests may be performed immediately in an emergency, or tests may be delayed as a condition is treated or monitored. A test may be suggested or become necessary when certain signs or symptoms appear. Due to changes in the way your body naturally functions through the course of a day, lab tests may need to be performed at a certain time of day. If you have prepared for a test by changing your food or fluid intake, lab tests may be timed in accordance with those changes. Timing of tests may be based on increased and decreased levels of medications, drugs or other substances in the body. The age or gender of the person being tested may affect when and how often a lab test is required. Chronic or progressive conditions may need ongoing monitoring through the use of lab tests. Conditions that worsen and improve may ...
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January 2015). "Hospital-associated transmission of Brucella melitensis outside the laboratory". Emerging Infectious Diseases. ... Brucella species survive well in aerosols and resist drying. Brucella and all other remaining biological weapons in the U.S. ... B. abortus is less virulent than B. melitensis and is primarily a disease of cattle. B. canis affects dogs. B. melitensis is ... Brucella suis, also referred to as swine brucellosis Wyatt HV (2014). "How did Sir David Bruce forget Zammit and his goats ?" ( ...
"Erythritol triggers expression of virulence traits in Brucella melitensis". Microbes and Infection. 15 (6-7): 440-449. doi: ... Erythritol is preferentially used by the Brucella spp. The presence of erythritol in the placentas of goats, cattle, and pigs ... has been proposed as an explanation for the accumulation of Brucella bacteria found at these sites. In the 19th and early 20th ...
Journal archive Moreno, Edgardo; Moriyon, Ignacio (8 January 2002). "Brucella melitensis: A nasty bug with hidden credentials ...
2015). "Recovery of a medieval Brucella melitensis genome using shotgun metagenomics". MBio. 5 (4): e01337-14. doi:10.1128/mBio ... recovering 200-year-old Mycobacterium tuberculosis genomes from human remains and a medieval Brucella genome sequence. With ...
Brucellosis is caused by different biotypes of Brucella abortus and B. melitensis. Other internal parasites include Fasciola ...
Professor Debono spent much time studying the brucella melitensis strain of brucellosis. In 1939 Debono assisted with the ...
"Whole-genome sequencing and antimicrobial resistance in Brucella melitensis from a Norwegian perspective". Scientific Reports. ...
Brucella melitensis is the cause of undulant fever in man and of abortion in goats. It is usually transmitted by goat's milk. ... later renamed Brucella melitensis). Themistocles Zammit, one of the members of the commission, discovered the carrier of the ... Brucella is the genus and Brucellaceae is the family of the bacteria which was named after him in recognition of his ... he led the Malta Fever Commission that identified the organism that caused the fever as a bacterium Micrococcus melitensis ( ...
These materials included anthrax, West Nile virus and botulism, as well as Brucella melitensis, and Clostridium perfringens. ... and new research activities involving Brucella and Crimean-Congo hemorrhagic fever virus. Despite diligent investigations since ...
Brucella melitensis is a pathogenic bacterium that causes fever and inflammation of the epididymis in sheep and cattle and can ... Brucella melitensis, and Escherichia coli. Malate synthase and the glyoxylate pathway is especially important to M. ... probing catalytic binding mode of acetyl-CoA malate synthase G in Brucella melitensis 16M". Biochemistry and Biophysics Reports ...
Moraxella catarrhalis, Brucella melitensis, Chlamydia pneumoniae, and Mycoplasma pneumoniae are generally susceptible to ... ducreyi Plague due to Yersinia pestis Tularemia Cholera Campylobacter fetus infections Brucellosis caused by Brucella species ( ...
... a potential drug target in Brucella melitensis 16M". Drug Design, Development and Therapy. 9: 1897-912. doi:10.2147/DDDT.S77020 ...
"Establishment of systemic Brucella melitensis infection through the digestive tract requires urease, the type IV secretion ... Pei J, Ficht TA (January 2004). "Brucella abortus rough mutants are cytopathic for macrophages in culture". Infection and ... Ugalde JE, Czibener C, Feldman MF, Ugalde RA (October 2000). "Identification and characterization of the Brucella abortus ... Ugalde JE, Comerci DJ, Leguizamón MS, Ugalde RA (November 2003). "Evaluation of Brucella abortus phosphoglucomutase (pgm) ...
... of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella ... 2002). "The genome sequence of the facultative intracellular pathogen Brucella melitensis". Proc. Natl. Acad. Sci. U.S.A. 99 (1 ... 2002). "The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts". Proc. Natl ... 2005). "Whole-genome analyses of speciation events in pathogenic Brucellae". Infection and Immunity. 73 (12): 8353-61. doi: ...
... aeruginosa Staphylococcus aureus Staphylococcus epidermidis Streptococcus agalactiae Streptococcus uberis Brucella melitensis ...
O. intermedium, O. anthropi, and Brucella melitensis can be distinguished on the basis of a multi-primer polymerase chain ... It is related to Brucella spp. and was first described by Velasco and others in 1998. It causes diseases in humans only rarely ... Velasco, J; Romero, C; López-Goñi, I; Leiva, J; Díaz, R; Moriyón, I (1998). "Evaluation of the relatedness of Brucella spp. and ... nov., a new species with a closer relationship to Brucella spp". International Journal of Systematic Bacteriology. 48 (3): 759- ...
After graduating, she volunteered to work in maternal health in southern India, where she contracted Brucella melitensis and ...
The facility was one of the first in Peru to be able to work with BSL-3 agents such as Brucella melitensis, Yersinia pestis and ...
... four structures from Brucella melitensis (3P0X, 3OQ8, 3EOL and 3E5B) and nine structures from Mycobacterium tuberculosis (1F61 ...
Bordetella bronchiseptica Bordetella pertussis Borrelia burgdorferi Brucella Brucella abortus Brucella melitensis Brucella suis ...
Brucella abortus Brucella melitensis Brucella suis Burkholderia mallei* (formerly Pseudomonas mallei) Burkholderia pseudomallei ...
Complete ORF sets have been cloned for a number of organisms including Brucella melitensis,Chlamydia pneumoniae,Escherichia ... "Construction and evaluation of an ORFeome-based Brucella whole-genome DNA microarray". Microbial Pathogenesis. 47 (4): 189-95. ...
... abortus A13334, 3,401 ORFs Brucella canis ATCC 23365, 3,408 ORFs Brucella melitensis 16M, 3,279 ORFs Brucella microti ... 3,193 ORFs Brucella pinnipedialis B2/94, 3,505 ORFs Brucella suis 1330, 3,408 ORFs Genome data for these and other Brucella ... Hence, the National Center for Biotechnology Information taxonomy includes most Brucella species under B. melitensis. The many ... Brucella species have been found primarily in mammals: Pathogenic Brucella species can cause abortion in female animals by ...
Since brucellosis threatens the food supply and causes undulant fever, Brucella suis and other Brucella species (B. melitensis ... of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella ... DelVecchio, VG; Kapatral, V; Elzer, P; Patra, G; Mujer, CV (20 December 2002). "The genome of Brucella melitensis". Veterinary ... Moreno, E; Moriyon, I (8 January 2002). "Brucella melitensis: a nasty bug with hidden credentials for virulence". Proceedings ...
... is a Gram-negative coccobacillus from the Brucellaceae family. Along with Brucella melitensis, it is responsible ... Brucella ovis, reviewed and published by Wikivet at http://en.wikivet.net/Brucella_ovis, accessed 24/08/2011. v t e v t e. ...
sRNA BM-sr0117 from Brucella melitensis was identified and shown to be bound to and cleaved by Bm-RNase III. AbcR and AbcR2 ( ... In Brucella melitensis eight novel sRNA genes were identified using bioinformatic and experimental approach. One of them ... 2015). "Identification of a Novel Small Non-Coding RNA Modulating the Intracellular Survival of Brucella melitensis". Front. ... They are often bound to the chaperone protein Hfq, which allows them to interact with mRNA(s). In Brucella suis 1330 RNA ...
... a Bioinformatics Resource Center funded by NIAID Brucella melitensis 16M genome information Type strain of Brucella melitensis ... Brucella melitensis is a Gram-negative coccobacillus bacterium from the Brucellaceae family. The bacterium causes ovine ... "brucellosis (Brucella melitensis)". www.cabi.org. Retrieved 22 March 2018. "Brucellosis in Humans: Symptoms, Treatment, Cause ... Moreno, E.; Moriyon, I. (8 January 2002). "Brucella melitensis: A nasty bug with hidden credentials for virulence". Proceedings ...
Brucella abortus MeSH B03.440.400.425.215.500.150 - Brucella canis MeSH B03.440.400.425.215.500.500 - Brucella melitensis MeSH ... Brucella abortus MeSH B03.660.050.070.100.150 - Brucella canis MeSH B03.660.050.070.100.500 - Brucella melitensis MeSH B03.660. ... 050.070.100.625 - Brucella ovis MeSH B03.660.050.070.100.750 - Brucella suis MeSH B03.660.050.070.700 - Ochrobactrum MeSH ... B03.440.400.425.215.500.625 - Brucella ovis MeSH B03.440.400.425.215.500.750 - Brucella suis MeSH B03.440.400.425.251 - ...
Brucella melitensis is the cause of undulant fever in man and of abortion in goats. It is usually transmitted by goat's milk. ... later renamed Brucella melitensis).[10] Themistocles Zammit, one of the members of the commission, discovered the cause of the ... Corbel, M.J.; Banai, M. (2015). "Brucella". Bergey's Manual of Systematics of Archaea and Bacteria. John Wiley & Sons. pp. 1-30 ... Brucella is the genus and Brucellaceae is the family of the bacteria which was named after him, in recognition of his ...
Brucellosis is caused by different biotypes of Brucella abortus and B. melitensis.[52] Other internal parasites include ...
viciae 3841 strain, and the Agrobacterium species A. vitis,A. tumefaciens, A. radiobacter and A. H13, Brucella species (B. ovis ... melitensis), in Bartonella species (i.e. B. henselae, B. clarridgeiae, B. tribocorum, B. quintana, B. bacilliformis, B. ... as well as in a broad spectrum of Brucella species (B. ovis, B. canis, B. abortus and B. microtis, and several viobars of B. ... Partial synteny of the αr45 genomic regions was observed in the Mesorhizobium and Brucella species where instead of aLysR ...
... as well as in a broad spectrum of Brucella species (B. ovis, B. canis, B. abortus and B. microtis, and several viobars of B. ... melitensis). αr14C RNA species are 115-125 nt long (Table 1) and share a well defined common secondary structure (Figure 1). ... The second group is formed by the first copy in the Chromosome I in the Brucellas genomes that share the gene upstream coding ... but lower bit-scores and are encoded by Brucella species (B. ovis, B. canis, B. abortus, B. microtis, and several biobars of B ...
He discovered Brucella abortus in 1897, which came to be known as Bang's bacillus. Bang's bacillus was the cause of the ... "Micrococcus melitensis" and "Bacillus abortus" by the American microbiologist Alice Evans]", Zeitschrift für ärztliche ...
A special case is the Brucella group, where primary automatic annotation over their genomes identified ORFs smaller than 30 aa ... melitensis). αr9C RNA species are 144-158 nt long (Table 1) and share a well defined common secondary structure consisting of ... as well as in a broad spectrum of Brucella species (B. ovis, B. canis, B. abortus and B. microtis, and several viobars of B. ... melitensis), Ochrobactrum anthropi and the Mesorhizobum species M. loti, M. ciceri and M. BNC. Parallel studies assessed Smr9C ...
To the last group correspond the αr15CII loci in the Brucella group (additional file 4) where only one of the genes could be ... melitensis). The Smr15C1 (115 nt) and Smr15C2 (121 nt) homologs are also encoded in tandem within the same IGR region of ... The second group includes the αr15CI1 loci in the Brucella species (additional file 2), which presented a very well conserved ... The αr15CI2 loci in the Brucella species (additional file 3) conform the group four and presented an up and downstream ...
9-941 chromosome I (NC_006932), Bmar7CI = Brucella melitensis biovar Abortus 2308 chromosome I (NC_007618), Bor7CI = Brucella ... 16M chromosome I (NC_003317), BaS19r7CI = Brucella abortus S19 chromosome 1 (NC_010742), Bm23457r7CI = Brucella melitensis ATCC ... Brucella suis ATCC 23445 chromosome I (NC_010169), Bm16Mr7CI = Brucella melitensis bv. 1 str. ... Partial synteny of the αr7 genomic regions was observed in the Mesorhizobium and Brucella group, where instead of the MarR ...
... a Bioinformatics Resource Center funded by NIAID Brucella melitensis 16M genome information Type strain of Brucella melitensis ... Brucella melitensis is a Gram-negative coccobacillus bacterium from the Brucellaceae family. The bacterium causes ovine ... "brucellosis (Brucella melitensis)". www.cabi.org. Retrieved 22 March 2018. "Brucellosis in Humans: Symptoms, Treatment, Cause ... Moreno, E.; Moriyon, I. (8 January 2002). "Brucella melitensis: A nasty bug with hidden credentials for virulence". Proceedings ...
Brucella melitensis bv. 3 str. Ether cont1.42, whole genome shotgun sequence Brucella melitensis bv. 3 str. Ether cont1.42, ... Brucella melitensis bv. 3 str. Ether cont1.42, whole genome shotgun sequence. GenBank: ACEI01000042.1 ...
Sulfur metabolism - Brucella melitensis M28 [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show description ...
... Sarwar Azam,1 Sashi ... Indels identified in the genome of Bm IND1 with respect to B. melitensis ATCC 23457. Indels were extracted using raw reads of ... Bm IND1 aligned to B. melitensis ATCC 23457 reference genome with the help of Bowtie2 and Samtools. SnpEFF was used to detect ...
Brucella melitensis. Mutation(s): 0 Gene Names: thyA, BMEI0608. EC: 2.1.1.45. ... Find proteins for P67042 (Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)) ... Crystal structure of Thymidylate synthase thyA from Brucella melitensis. Abendroth, J., Edwards, T.E., Seattle Structural ... Crystal structure of Thymidylate synthase thyA from Brucella melitensis. *DOI: 10.2210/pdb3IX6/pdb ...
... melitensis 16M, B. melitensis M28, B. melitensis ATCC 23457, B. melitensis M5-90, B. melitensis NI, B. melitensis Ether, and B. ... melitensis M28, B. melitensis ATCC 23457, B. melitensis M5-90, B. melitensis NI, B. melitensis Ether, and B. abortus 2308 for ... melitensis M28, B. melitensis M5-90, and B. melitensis NI, whereas B. melitensis Ether and B. melitensis 16M branched ... Completely sequenced genomes of B. melitensis such as B. melitensis 16M, B. melitensis M28, B. melitensis ATCC23457, and B. ...
... SKU: GL-1002 Retail Price: $24.50. Our LOW Price: $18.33. You Save: $6.17 (25%). Availability: ... Home » Medical Equipment & Supplies » Instruments & Disposables » Urine Reagent Strips, Individual Packs » Brucella Melitensis ...
... of the vegetation with the echocardiography and with the production from the multiple blood culture of brucella melitensis and ... We present a rare case of brucella endocarditis, forming a vegetation on the mitral valve. The definitive diagnosis has been ...
What is Brucella melitensis? Meaning of Brucella melitensis medical term. What does Brucella melitensis mean? ... Looking for online definition of Brucella melitensis in the Medical Dictionary? Brucella melitensis explanation free. ... Related to Brucella melitensis: Brucella canis, Brucella abortus, Brucella suis. Bru·cel·la me·li·ten·sis. (brū-selă mel-i- ... Brucella melitensis as causative agent for neck abscess in an endemic area.. Brucella Melitensis As Causative Agent for Neck ...
Immunogenicity evaluation of plasmids encoding Brucella melitensis Omp25 and Omp31 antigens in BALB/c mice.. Shojaei M1, ... Immunogenicity evaluation of plasmids encoding Brucella melitensis Omp25 and Omp31 antigens in BALB/c mice ... Immunogenicity evaluation of plasmids encoding Brucella melitensis Omp25 and Omp31 antigens in BALB/c mice ... Immunogenicity evaluation of plasmids encoding Brucella melitensis Omp25 and Omp31 antigens in BALB/c mice ...
Recovery of a Medieval Brucella melitensis Genome Using Shotgun Metagenomics. Gemma L. Kay, Martin J. Sergeant, Valentina ... Recovery of a Medieval Brucella melitensis Genome Using Shotgun Metagenomics. Gemma L. Kay, Martin J. Sergeant, Valentina ... Recovery of a Medieval Brucella melitensis Genome Using Shotgun Metagenomics. Gemma L. Kay, Martin J. Sergeant, Valentina ... Recovery of a Medieval Brucella melitensis Genome Using Shotgun Metagenomics Message Subject (Your Name) has forwarded a page ...
Presence of two independent chromosomes in the Brucella melitensis 16M genome.. S Michaux, J Paillisson, M J Carles-Nurit, G ... Presence of two independent chromosomes in the Brucella melitensis 16M genome.. S Michaux, J Paillisson, M J Carles-Nurit, G ... Presence of two independent chromosomes in the Brucella melitensis 16M genome.. S Michaux, J Paillisson, M J Carles-Nurit, G ... Presence of two independent chromosomes in the Brucella melitensis 16M genome. Message Subject (Your Name) has forwarded a page ...
Brucella antigen in liver and spleen from Asian badgers infected with Brucella melitensis, northwestern China. A) Brucella ... Brucella antigen in liver and spleen from Asian badgers infected with Brucella melitensis, northwestern China. A) Brucella ... Wang Q, Zhao S, Wureli H, Xie S, Chen C, Wei Q, et al. Brucella melitensis and B. abortus in eggs, larvae and engorged females ... Isolation of Brucella melitensis from alpine ibex. J Wildl Dis. 1998;34:400-2. DOIPubMedGoogle Scholar ...
1 and H38 Brucella melitensis vaccines. Brucella melitensis. CEC seminar, Brussels, November 1984, 215-227; [Current Topics in ... Brucella melitensis in Greece: current situation report. Brucella melitensis. CEC seminar, Brussels, November 1984, 43-46; [ ... Brucella melitensis mainly infects sheep and goats, and in areas where B. melitensis is enzootic it is the major cause of ... Brucella melitensis primarily affects the reproductive tract of sheep and goats, and B. melitensis infection is characterized ...
sp,Q8YHC6,LON_BRUME Lon protease OS=Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094) OX=224914 GN=lon PE=3 ... cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › BrucellaBrucella ... Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094). ,p>This subsection of the ,a href="http://www.uniprot.org ...
Keywords: brucellosis melitensis, CIED infection, ELISA, brucellosis endocarditis, Brucella melitensis endocarditis ... We report a case of Brucella melitensis infection of CIED where, contrary to most authorities recommending removal of device, ... At the time of discharge, the patients Brucella titer was ,1:160. The patient was discharged with a 2-month supply of rifampin ... A case of Brucella melitensis endocarditis in a patient with cardiovascular implantable electronic device Wasl Al-Adsani, Ali ...
It is necessary for persistence in mice, essential for intracellular growth of Brucella and required for expression of the type ... IV secretion system VirB and therefore plays a role in adaptation of Brucella to its intracellular host environment. ... "The stringent response mediator Rsh is required for Brucella melitensis and Brucella suis virulence, and for expression of the ... "The stringent response mediator Rsh is required for Brucella melitensis and Brucella suis virulence, and for expression of the ...
5-Tetrahydropyridine-2-carboxylate N-Succinyltransferase from Brucella melitensis biovar abortus 2308 ... Crystal structure of 2,3,4,5-Tetrahydropyridine-2-carboxylate N-Succinyltransferase from Brucella melitensis biovar abortus ...
Rapid identification of Yersinia pestis and Brucella melitensis by chip-based continuous flow PCR Author(s): Michael Dietzsch; ... To combat the threat of biological agents like Yersinia pestis and Brucella melitensis in bioterroristic scenarios requires ... a system for rapid amplification of specific genetic markers for the identification of Yersinia pestis and Brucella melitensis ...
Brucella serum (Remel Inc., Lenexa, Kans.). Strains identified as B. melitensis were stored in brucella broth (Remel Inc.) at − ... Brucella strains were isolated from 16 (1.24%) of the cultures, and all of them were identified as B. melitensis. Nine (56.25 ... Synergy Tests by E Test and Checkerboard Methods of Antimicrobial Combinations against Brucella melitensis. Gani Orhan, Aysen ... Synergy Tests by E Test and Checkerboard Methods of Antimicrobial Combinations against Brucella melitensis ...
In 2011, Brucella melitensis biovar 1, a biotype not reported in Italy since 1995, was isolated in one flock in the region. The ... MLVA as an Epidemiological Tool To Trace Back Brucella melitensis Biovar 1 Re-Emergence in Italy.. [F De Massis, M Ancora, M ... The knowledge of Brucella spp. prevailing genotypes in a territory represents an important epidemiological tool to formulate ... Brucellosis is an important zoonosis caused by Brucella spp., still prevalent in most areas of the world. Brucellosis control ...
However, it is well known that the virulence of B. melitensis is more than B. abortus, but there is not any strong evidence to ... To this end, five B. abortus and five B. melitensis strains isolated from cows and human, were subjected to 39, 40 and 42 ° C ... The heat shock responses in B. abortus and B. melitensis point to the higher production of a 60 kDa protein (hsp60) in both ... Effects of Brucella abortus Biotype 1 Infection on the Reproductive Performance of Sprague-Dawley Rats ...
FARINA, R. Current serological methods in B. melitensis diagnosis. In: PLOMMET, M., VERGER, J.M. (Eds). Brucella melitensis. ... 1 de B. melitensis.. Neste estudo, utilizou-se a vacina viva da estirpe Rev.1 de B. melitensis contendo 1´ 109 UFC/dose vacinal ... Evaluation of serological tests for the diagnosis of Brucella melitensis infection of goats. J. Clin. Microbiol., v.32, p.1159- ... A brucelose caprina e ovina causada pela espécie Brucella melitensis é uma doença infecciosa que se caracteriza por aborto e ...
Deletion of purE attenuates Brucella melitensis infection in mice.. R M Crawford, L Van De Verg, L Yuan, T L Hadfield, R L ... Deletion of purE attenuates Brucella melitensis infection in mice.. R M Crawford, L Van De Verg, L Yuan, T L Hadfield, R L ... Deletion of purE attenuates Brucella melitensis infection in mice.. R M Crawford, L Van De Verg, L Yuan, T L Hadfield, R L ... Deletion of purE attenuates Brucella melitensis infection in mice. Message Subject (Your Name) has forwarded a page to you from ...
In China, brucellosis is recognized as a re-emerging disease mainly caused by Brucella melitensis specie. To better understand ... In China, brucellosis is recognized as a re-emerging disease mainly caused by Brucella melitensis specie. To better understand ... the currently endemic B. melitensis strains in China, three Brucella genotyping methods were applied to 110 B. melitensis ... the currently endemic B. melitensis strains in China, three Brucella genotyping methods were applied to 110 B. melitensis ...
CLINICAL AND LABORATORY OBSERVATIONS ON BRUCELLA MELITENSIS IN EGYPT: STUDY OF 100 CASES1 JOHN H. KILLOUGH, Ph.D., M.D.; GORDON ... CLINICAL AND LABORATORY OBSERVATIONS ON BRUCELLA MELITENSIS IN EGYPT: STUDY OF 100 CASES1. Ann Intern Med. ;39:222-229. doi: ...
Serological and Nucleic Acid Based Detection of Brucellosis in Livestock Species and Molecular Characterization of Brucella ... melitensis Strains Isolated from Pakistan.(Report) by International Journal of Agriculture and Biology; Business, ... Differentiation of Brucella abortus bv. 1, 2 and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. J. Clin ... Detection of Brucella melitensis and Brucella abortus by DNA amplification. J. Trop. Med. Hyg., 95: 271-275. Barbier, T., C. ...
... blood culture grown with Brucella melitensis). The patient received specific treatment of the three infections and recovered. ... oa Case Report: Visceral Leishmaniasis with Salmonella Paratyphi and Brucella melitensis Coinfection as a Cause of Persistent ... blood culture grown with Brucella melitensis). The patient received specific treatment of the three infections and recovered. ... 2 Brucella Department, Veterinary Research Institute, Khartoum, Sudan; 3 Institute of Tropical Medicine, Antwerp, Belgium; 4 ...
Rapid diagnosis of Brucella melitensis in blood: some operational characteristics of the BACT/ALERT. J Clin Microbiol30:222-224 ... Isolation rates of Brucella melitensis in an endemic area and implications for laboratory safety. Eur J Clin Microbiol Infect ... Factors affecting detection of Brucella melitensis by BACTEC NR730, a nonradiometric system for hemocultures. J Clin Microbiol ... Overall, 31 cultures (35.2%) drawn from 19 patients (38.0%) grew Brucella organisms (all later identified as B. melitensis). ...
  • Brucella melitensis primarily affects the reproductive tract of sheep and goats, and B. melitensis infection is characterized by abortion, retained placenta and, to a lesser extent, impaired fertility. (au-ibar.org)
  • We report a case of Brucella melitensis infection of CIED where, contrary to most authorities recommending removal of device, because of the patient's multiple comorbid conditions and age, an attempt was made to keep the device and place the patient on lifelong prophylaxis treatment. (dovepress.com)
  • Deletion of purE attenuates Brucella melitensis infection in mice. (asm.org)
  • In the next few years, MLVA typing will certainly remain an important epidemiological tool for Brucella infection analysis, as it displays a high discriminatory ability and achieves result largely in agreement with WGS-SNP-based typing. (frontiersin.org)
  • Brucella strains infect macrophages and establish chronic infection by altering host life activities including apoptosis and autophagy. (ijbs.com)
  • Taken together, these data provide valuable framework on Brucella induced miRNA expression in RAW264.7 cells, and suggest that Brucella may establish chronic infection by regulating miRNA expression profile. (ijbs.com)
  • Besides, apoptosis of macrophages is inhibited, facilitating Brucella ′s latent infection [ 8 , 9 ]. (ijbs.com)
  • These studies provide important clues to the mechanism how Brucella strains establish chronic infection. (ijbs.com)
  • Aim: To identify and evaluate the shedding routes and patterns following experimentally-induced Brucella melitensis infection in dexamethasone-treated and transport-stressed goats. (semanticscholar.org)
  • To better understand the universe of antibody responses that develop after B. melitensis infection, a protein microarray was fabricated containing 1,406 predicted B. melitensis proteins. (elsevier.com)
  • Evaluation of azithromycin, trovafloxacin and grepafloxacin as prophylaxis against experimental murine Brucella melitensis infection. (tropmedres.ac)
  • The prophylactic potential of the azalide azithromycin as well as the fluoroquinolones trovafloxacin and grepafloxacin was assessed for the control of infection with Brucella melitensis in an experimental mouse model, determined by reduction in splenic bacterial burden. (tropmedres.ac)
  • Overall, the data confirm the potential utility of azithromycin in the prophylaxis of brucellosis and suggest that neither trovafloxacin nor grepafloxacin would likely be valuable for post-exposure prophylaxis of Brucella infection. (tropmedres.ac)
  • Many animals are susceptible to infection by Brucella species, such as goats, cows, pigs and sometimes canines. (biomedcentral.com)
  • Brucella infection can occur via genital mucosa, inhalation, digestion and even via injured skin. (biomedcentral.com)
  • Human infection is usually through the inspiration of aerosols containing Brucella , ingestion of unpasteurized milk or undercooked meat from infected animals, or close contact with their secretions, baby animals, milk and excrement. (biomedcentral.com)
  • Infection with Brucella abortus, B. melitensis and B. suis Article 8.4.3. (samsungupdate.com)
  • Country or zone historically free from infection with Brucella in specified animal categories A country or zone may be considered free from infection with Brucella in specified animal categories when: 1 infection with Brucella in animals is a notifiable disease in the. (samsungupdate.com)
  • Brucella melitensis , although not a common infection in the United States, can be found in travelers from endemic areas. (shmabstracts.com)
  • We report herein a woman with splenic abscess due to acute Brucella melitensis infection who was successfully treated with antibiotics alone. (sdu.edu.tr)
  • While infection with Brucella species often presents with non-localizing systemic symptoms, genitourinary manifestations are uncommonly diagnosed. (ispub.com)
  • Four species, Brucella melitensis , Brucella abortus , Brucella canis , and Brucella suis , are known to cause infection in humans. (ispub.com)
  • VirB2 can affect expression levels of Ubc9, thereby increasing intracellular survival of Brucella in macrophages at the late stage of infection. (bvsalud.org)
  • Collectively, our results demonstrate that B. melitensis 16M may use the VirB IV secretion system of Brucella to interact with SUMO-related proteins during infection of host cells, which interferes with SUMO function and promotes pathogen survival in host cells. (bvsalud.org)
  • We created a curated ready-to-use species-specific wgMLST scheme enrolling a panel of 2656 targets (http://doi.org/10.5281/zenodo.3575026) and used this schema to perform a retrospective analysis of the genetic relatedness among B. melitensis strains causing human infection in Portugal (a country where brucellosis is an endemic disease) from 2010 to 2018. (cdc.gov)
  • Brucellosis, caused by the genus Brucella bacterium , is a well-known infection among domestic animals. (ac.ir)
  • Considering the serious economic and medical consequences of this infection, various preventive efforts have been made through using recombinant vaccines, based on outer membrane protein (OMP) antigens of Brucella species. (ac.ir)
  • La brucellose, une infection causée par des bactéries du genre Brucella, est très répandue chez les animaux domestiques. (ac.ir)
  • Compte tenu des conséquences économiques et sanitaires de cette infection, des mesures de prévention variées ont été mises en place, comme notamment l'usage de vaccins recombinants à partir d'antigènes isolés des membranes cellulaires externes des espèces de Brucella. (ac.ir)
  • Vaccination with the recombinant Brucella outer membrane protein 31 or a derived 27-amino-acid synthetic peptide elicits a CD4+ T helper 1 response that protects against Brucella melitensis infection. (ac.ir)
  • The Brucella abortus (rough LPS Brucella) vaccine, developed for bovine brucellosis and licensed by the USDA Animal Plant Health Inspection Service, has shown protection for some swine and is also effective against B. suis infection, but there is currently no approved vaccine for swine brucellosis. (wikipedia.org)
  • 0.025) in loops inoculated with virulent B. melitensis 16M compared with controls between 15 min and 4 h post-infection. (omicsdi.org)
  • The New York City Health Department issued a health alert Friday after two recent imported cases of the zoonotic bacterial infection, Brucella melitensis were reported in the city. (outbreaknewstoday.com)
  • This helps to provide a scientific basis for the prevention and control of Brucella infection in pregnant women. (biomedcentral.com)
  • Crucial role of gamma interferon-producing CD4+ Th1 cells but dispensable function of CD8+ T cell, B cell, Th2, and Th17 responses in the control of Brucella melitensis infection in mice. (ac.ir)
  • 9. Al-Mariri A. Protection of BALB/c mice against Brucella melitensis 16 M infection induced by vaccination with live Escherchia coli expression Brucella P39 protein. (ac.ir)
  • Based on the observation that M2a-like macrophages are induced in the spleen during the chronic phase of B. abortus infection in mice and are strongly infected in vitro, it has been suggested that M2a macrophages could be a potential in vivo niche for Brucella. (duhnnae.com)
  • Blasco JM, Molina-Flores B (2011) Control and eradication of Brucella melitensis infection in sheep and goats. (springer.com)
  • Six classical species were initially recognized within the genus Brucella, namely, B . abortus , B . melitensis , B . suis , B . ovis , B . canis , and B . neotomae [ 2 ]. (hindawi.com)
  • Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis. (thefreedictionary.com)
  • Brucella abortus (cattle), Brucella melitensis (goat and sheep), Brucella suis (pigs), Brucella canis (dogs), Brucella ovis (sheep), and Brucella neotomae (rodents). (frontiersin.org)
  • For host preference, B. melitensis is associated with sheep and goats, B. abortus with cattle, B. suis with pigs, B. ovis with sheep only, and B. canis with dogs (Verger et al. (thefreelibrary.com)
  • MLVA Genotyping of Brucella melitensis and Brucella abortus Isolates from Different Animal Species and Humans and Identification of Brucella suis Vaccine Strain S2 from Cattle in China. (ebscohost.com)
  • Clinical and Diagnostic Aspects of Relapsing Meningoencephalitis due to Brucella suis. (ebscohost.com)
  • B.abortus , B.melitensis , B.suis , B.ovis , B.canis , and B.neotomae , of which B.melitensis is the most widely distributed [ 4 ]. (ijbs.com)
  • 27/12/33 · Three types of the bacteria that cause brucellosis - Brucella abortus, Brucella melitensis and Brucella suis - are designated as select agents. (samsungupdate.com)
  • Among these mAbs, 7A3 (IgG1), 5B1 (IgG2a), 2C1 (IgG2a) and 5B3 (IgG2a) reacted with differently conserved linear epitopes of B. melitensis , B. ovis , B. suis and B. canis strains, while 5H3 (IgG2a) highly reacted with a conformational epitope of Omp31 when tested with several immunoassays. (biomedcentral.com)
  • Brucella suis is a bacterium that causes swine brucellosis, a zoonosis that affects pigs. (wikipedia.org)
  • Since brucellosis threatens the food supply and causes undulant fever, Brucella suis and other Brucella species (B. melitensis, B. abortis, B. ovis, B. canis) are recognized as potential agricultural, civilian, and military bioterrorism agents. (wikipedia.org)
  • Brucella suis is a Gram-negative, facultative, intracellular coccobacillus, capable of growing and reproducing inside of host cells, specifically phagocytic cells. (wikipedia.org)
  • Brucella suis is differentiated into five biovars (strains), where biovars 1-3 infect wild boar and domestic pigs, and biovars 1 and 3 may cause severe diseases in humans. (wikipedia.org)
  • Summary: Brucella suis encounters a macrophage, but no oxidative burst occurs. (wikipedia.org)
  • Brucella melitensis , the most virulent species in humans, primarily affects goats and the zoonotic transmission occurs by ingestion of unpasteurized milk products or through direct contact with fetal tissues. (hindawi.com)
  • Brucellosis is an endemic disease in India and the country experienced a sharply increasing rate of human brucellosis in recent years, and the species of main concern is B . melitensis . (hindawi.com)
  • It is the type species of the genus Brucella. (thefreedictionary.com)
  • a similar organism to the other brucellae except that it is inhibited in growth by a 10% concentration of CO 2 , a cultural enhancement for the other species. (thefreedictionary.com)
  • Human brucellosis is most commonly caused by the species Brucella melitensis and is usually acquired through ingestion of unpasteurized dairy products or, less commonly, through ingestion of infected meat or direct occupational contact with animals ( 1 ). (asm.org)
  • Mapping the restriction fragments of the Brucella melitensis 16M genome with a new restriction endonuclease, PacI, which cut the DNA into only eight fragments, indicated that this species contains two unique and independent replicons of about 2,100 and 1,150 kb. (asm.org)
  • We used the partial omp 22 gene (238 bp) encoding 22-kD outer membrane protein to identify the Brucella genus and the IS 711 gene to identify Brucella species. (cdc.gov)
  • The heat shock responses in B. abortus and B. melitensis point to the higher production of a 60 kDa protein (hsp60) in both bacterial species, especially in B. abortus . (scialert.net)
  • More recently, additional species have been suggested originating from a wide range of hosts, Brucella ceti (cetaceans), Brucella pinnipedialis (seals), Brucella microti (voles), Brucella papionis (baboons), and Brucella vulpis (foxes) ( 10 - 12 ). (frontiersin.org)
  • Serological and Nucleic Acid Based Detection of Brucellosis in Livestock Species and Molecular Characterization of Brucella melitensis Strains Isolated from Pakistan. (thefreelibrary.com)
  • Genus Brucella is classified into different species based on phenotypic differences and host preferences. (thefreelibrary.com)
  • 1987). However, since 1994, several new Brucella species have been isolated from marine mammals (Foster et al. (thefreelibrary.com)
  • 2008). More recently, several species of Brucella have been isolated from human (Scholz et al. (thefreelibrary.com)
  • Brucella melitensis (biovars 1 and 3) is the predominant species, associated with sporadic cases and outbreak in humans. (ebscohost.com)
  • Thirty-five serum samples and six hygroma fluid samples were collected from sexually mature cattle in one herd with clinical signs of brucellosis (abortion and hygromas) in the Western Region of the Gambia in order to isolate and characterise Brucella species. (ebscohost.com)
  • B. melitensis is also considered the most pathogenic species for humans ( 9 ). (asm.org)
  • Since Brucella species are intracellular pathogens, it is obvious that combined antibiotic therapy with confirmed intracellular activity is the best therapeutic regimen in brucellosis. (ijmm.org)
  • B. melitensis is the most common species of Brucella in human illnesses, with some estimates suggesting that it is responsible for 70% of all infections. (samsungupdate.com)
  • Among the six species of genus Brucella, Br. (samsungupdate.com)
  • 06/08/32 · Brucella melitensis is one of the six species that causes Brucellosis, which can be described as a fatal zoonotic disease that affects multiple body systems. (samsungupdate.com)
  • Detection and identification of Brucella species are essential to prevent or treat brucellosis in humans and animals. (biomedcentral.com)
  • These potent monoclonal antibodies can be used for identifying Omp31 antigens or detecting B. melitensis and other Brucella species beyond B. abortus in vitro or in vivo. (biomedcentral.com)
  • Some of these antibodies presented high reactivity with different epitopes of Omp31, suggesting potential capacity to identify Omp31 antigens of Brucellae or to detect B. melitensis or other Brucella species beyond B. abortus . (biomedcentral.com)
  • During this time, the patient's wife was diagnosed with osteomyelitis secondary to Brucella species. (ispub.com)
  • The results of phylogenetic analysis showed that Omp31 is almost similar in different Brucella species. (ac.ir)
  • 6. Bowden RA, Cloeckaert A, Zygmunt MS, Bernard S, Dubray G. Surface exposure of outer membrane protein and lipopolysaccharide epitopes in Brucella species studied by enzyme-linked immunosorbent assay and flow cytometry. (ac.ir)
  • The aim of this study was to identify the species and determine the antimicrobial susceptibility pattern of Brucella strains isolated from clinical specimens, from Qatar. (ru.nl)
  • In Brucella abortus and Brucella melitensis, the major species infecting domestic ruminants, the smooth lipopolysaccharide (S-LPS) is a virulence factor. (figshare.com)
  • The bacterium causes ovine brucellosis, along with Brucella ovis. (wikipedia.org)
  • Role of the Omp25/Omp31 family in outer membrane properties and virulence of Brucella ovis. (ac.ir)
  • Brucellae are Gram-negative, facultative, intracellular bacteria that can infect a wide range of domestic and wild animals as well as humans. (hindawi.com)
  • Brucella mechanisms and virulence factors that mediate invasion and intracellular persistence have been poorly characterized. (hindawi.com)
  • It is necessary for persistence in mice, essential for intracellular growth of Brucella and required for expression of the type IV secretion system VirB and therefore plays a role in adaptation of Brucella to its intracellular host environment. (uniprot.org)
  • Brucellosis is caused by intracellular Gram-negative bacteria belonging to Brucella genus. (frontiersin.org)
  • Brucellosis is a zoonosis of global socio-economic importance caused by Gram negative facultative intracellular bacteria of genus Brucella. (thefreelibrary.com)
  • Fluoroquinolones and newer macrolides have good anti- Brucella activity in vitro ( 1 , 9 , 12 ) and reach high intracellular concentrations, but their in vitro activity may predict efficacy poorly, since brucellae survive in compartments that are inaccessible or hostile to antimicrobial activity. (asm.org)
  • Brucella organisms are Gram-negative facultative intracellular bacteria that cause Brucellosis . (ijbs.com)
  • Brucellae are Gram-negative, facultative intracellular, coccobacillus bacteria that cause Brucellosis , a zoonotic disease affecting humans and animals worldwide [ 1 ]. (ijbs.com)
  • Brucella 16M activated SUMO1/Ubc9 expression in a time-dependent manner, and Brucella 16M intracellular survival was inhibited by SUMO1/Ubc9 overexpression and promoted by SUMO1/Ubc9 depletion. (bvsalud.org)
  • Additionally, intracellular survival of the 16M△VirB2 mutant was lower than that of Brucella 16M (p (bvsalud.org)
  • Similar to many other intracellular dwelling bacteria, infections caused by Brucella are difficult to treat, and hence any attempt at identifying new and common therapeutic targets would prove beneficial for the purpose of curing infections caused by the intracellular bacteria. (ac.ir)
  • Brucella melitensis and M. tuberculosis go through similar intracellular processes and interestingly two of the investigated genes, i.e. (ac.ir)
  • Brucella are facultative intracellular Gram-negative coccobacilli that chronically infect humans as well as domestic and wild-type mammals, and cause brucellosis. (duhnnae.com)
  • Immunogenicity evaluation of plasmids encoding Brucella melitensis Omp25 and Omp31 antigens in BALB/c mice. (nih.gov)
  • To detect Brucella antigens, we used immunohistochemical staining of liver and spleen tissue sections by pipetting mouse anti- Brucella melitensis IgG diluted 1:100 in 30% bovine serum albumin/phosphate-buffered saline onto each section. (cdc.gov)
  • Brucella abortus and B. melitensis antigens were used in parallel on the National Standard Brucella abortus antiserum and on field sera coming from cattle where practically exclusively B. abortus biotypes 1 and 2 have been isolated over the last 11 years. (up.ac.za)
  • The use of the dual antigens could identify herds which are infected only with A-antigen predominant brucellae but would not be reliable for classifying individual animals. (up.ac.za)
  • Immunogenicity evaluation of plasmids encoding Brucella melitensis Omp25 and Omp31 antigens in BALB/c mice', Iranian Journal of Basic Medical Sciences , 21(9), pp. 957-964. (ac.ir)
  • 7. Zygmunt MS, Debbarh HS-A, Cloeckaert A, Dubray G. Antibody response to Brucella melitensis outer membrane antigens in naturally infected and Rev1 vaccinated sheep. (ac.ir)
  • Due to negative aspects of live attenuated vaccines, much attention has been focused on finding Brucella-protective antigens to introduce them as potential subunit vaccine candidates. (ac.ir)
  • 6. Durward MA, Harms J, Magnani DM, Eskra L, Splitter GA. Discordant Brucella melitensis antigens yield cognate CD8+ T cells in vivo . (ac.ir)
  • Indels identified in the genome of Bm IND1 with respect to B. melitensis ATCC 23457. (hindawi.com)
  • Indels were extracted using raw reads of Bm IND1 aligned to B. melitensis ATCC 23457 reference genome with the help of Bowtie2 and Samtools. (hindawi.com)
  • For more detailed genetic characterization, we carried out whole genome sequencing and comparative genome analysis of one of the B . melitensis isolates, Bm IND1. (hindawi.com)
  • Genome sequencing and analysis of Brucella spp. (hindawi.com)
  • We obtained 6.5-fold coverage of a Brucella melitensis genome. (asm.org)
  • Despite the relatively low coverage, we were able to use information from single-nucleotide polymorphisms to place the medieval pathogen genome within a clade of B. melitensis strains that included the well-studied Ether strain and two other recent Italian isolates. (asm.org)
  • By sequencing DNA extracted from medieval human remains through shotgun metagenomics, without target-specific capture or amplification, we have obtained a draft genome sequence of an ~700-year-old Brucella melitensis strain. (asm.org)
  • Presence of two independent chromosomes in the Brucella melitensis 16M genome. (asm.org)
  • Whole-genome sequence (WGS)-single-nucleotide polymorphism (SNP)-based typing and phylogenetic analysis resolved Chinese B. melitensis strains into five clusters, reflecting the existence of multiple lineages among these Chinese B. melitensis strains. (frontiersin.org)
  • We sequenced the full genome and characterised the genetic diversity of two Brucella melitensis isolates from Malaysia and the Philippines. (biomedcentral.com)
  • The B. melitensis genome sequences, and two B. abortus strains used as the outgroup strains, yielded a total of 13,728 SNP sites. (biomedcentral.com)
  • Phylogenetic analysis using whole-genome SNPs and geographical distribution of the isolates revealed spatial clustering of the B. melitensis isolates into five genotypes, I, II, III, IV and V. The Mediterranean strains, identified as genotype I, occupied the basal node of the phylogenetic tree, suggesting that B. melitensis may have originated from the Mediterranean regions. (biomedcentral.com)
  • Phylogeographic reconstruction of the history of B. melitensis global spread at a finer scale by using whole-genome SNP analyses supported the origin of all B. melitensis strains from the Mediterranean region. (biomedcentral.com)
  • The possible global distribution of B. melitensis following the ancient trade routes was also consistent with whole-genome SNP phylogeny. (biomedcentral.com)
  • This study confirms whole-genome based SNP-analysis as a powerful tool for accurate typing of B. melitensis. (uni-muenchen.de)
  • We genotyped B. melitensis isolates from Omani camels using whole genome SNP assays and VNTRs to provide context for regional brucellosis cases. (cdc.gov)
  • Host-Brucella interactions and the Brucella genome as tools for subunit antigen discovery and immunization against brucellosis. (ac.ir)
  • We performed multilocus sequence typing of four B . melitensis strains isolated from naturally infected goats from India. (hindawi.com)
  • Two different synergy testing methods, the checkerboard and the E test methods, were used to compare the in vitro efficacies of various antimicrobial combinations against 16 Brucella melitensis strains isolated from blood cultures. (asm.org)
  • To this end, five B. abortus and five B. melitensis strains isolated from cows and human, were subjected to 39, 40 and 42 ° C heat shocks. (scialert.net)
  • Phenotypic and genotypic characterisation of Brucella strains isolated from cattle in the Gambia. (ebscohost.com)
  • This is the first report of a detailed genetic investigation of an extensive collection of B. melitensis strains isolated from human cases in Germany. (uni-muenchen.de)
  • We isolated Brucella melitensis biovar 3 from the spleen of an Asian badger ( Meles leucurus ) in Nilka County, northwestern China. (cdc.gov)
  • MLVA as an Epidemiological Tool To Trace Back Brucella melitensis Biovar 1 Re-Emergence in Italy. (sigmaaldrich.com)
  • In 2011, Brucella melitensis biovar 1, a biotype not reported in Italy since 1995, was isolated in one flock in the region. (sigmaaldrich.com)
  • Brucella melitensis biovar 3 was isolated from the tissue specimens uterus and/or lymph nodes. (samsungupdate.com)
  • In this work, the patient living in Shanxi Province in China who had suffered a spontaneous abortion was underwent pathogen detection and Brucella melitensis biovar 3 was identified. (biomedcentral.com)
  • This is the first reported case of miscarriage resulting from Brucella melitensis biovar 3 isolated from a pregnant woman who was infected through unpasteurized milk in China. (biomedcentral.com)
  • In goats and sheep, B. melitensis can cause abortion, stillbirth, and weak offspring for the first gestation after the animal is infected. (wikipedia.org)
  • Brucella melitensis , with a reservoir in sheep, goats, and camels may cause severe, acute disease and disabling complications. (thefreedictionary.com)
  • We homogenized spleen samples of badgers and the 14 aborted sheep fetuses and inoculated these homogenates onto individual Brucella agar plates, which we then incubated at 37°C in an atmosphere of 5% CO 2 for 5 days. (cdc.gov)
  • In addition, we isolated B. melitensis biotype 3 from badger no. 2 and 5 aborted sheep fetuses according to phenotypic identification ( Appendix Table). (cdc.gov)
  • Although B. melitensis infects mainly sheep and goats it is a zoonose that plays a significant role in the national economy and the public health of many developing countries. (au-ibar.org)
  • Most Mediterranean countries have large numbers of flocks of sheep and goats infected with B. melitensis (Yantzis, 1984). (au-ibar.org)
  • B. melitensis was originally found as a pathogen that mainly affected goats and sheep, which caused a decrease in fertility, loss of young, and a decrease in milk production. (samsungupdate.com)
  • Brucella melitensis (B. melitensis) is an infectious bacterial disease that can affect most domestic animals, but goats and sheep are especially susceptible. (ca.gov)
  • Traditionally found in goats and sheep, Brucella melitensis is increasingly recognized as infecting camels. (cdc.gov)
  • We identified a lineage of B. melitensis circulating in camels as well as in goats, sheep, and cattle in Oman. (cdc.gov)
  • Humoral immune responses of Brucella-infected cattle, sheep, and goats to eight purified recombinant Brucella proteins in an indirect enzyme-linked immunosorbent assay. (ac.ir)
  • The aim of this study was to assess in vitro antimicrobial susceptibility of Brucella melitensis isolates isolated from naturally infected sheep cases in an area where human brucellosis is endemic, focusing on rifampin (RIF), streptomycin (SM), ciprofioxacin (CPFX), trimethoprim/sulfamethoxazole (TMP/SMZ), gentamicin (GM) and tetracycline (TC) and on 11 other antimicrobials. (yyu.edu.tr)
  • Annex I.b: Programme for the eradication of bovine Tuberculosis, bovine Brucellosis or sheep and goat Brucellosis (B. melitensis) submitted for obtaining EU cofinancing. (springer.com)
  • 2014. Brucella canis Not a Select Agent N/A N/A 2 hours 24 hours or 7 days Brucella ceti, pinnipedialis Not a Select Agent N/A N/A 2 hours 24 hours or 7 days Case Report Form Health departments and providers are strongly encouraged to use the approved case report form to report brucellosis cases to the Bacterial Special Pathogens Branch. (samsungupdate.com)
  • Collectively, these finding suggested that the pcDNA3.1/Hygro DNA vaccines encoding Omp25 and Omp31 genes and divalent plasmid were able to induce both humoral and cellular immunity, and had the potential to be a vaccine candidate for prevention of B. melitensis infections. (nih.gov)
  • Therefore, hsp-60 can be a good antigenic candidate for engineering subunit vaccine against Brucella , as well as for ELISA test development. (scialert.net)
  • The live B. melitensis Rev.1 strain is considered the best vaccine available for the prophylaxis of brucellosis in small ruminants, especially when used at the standard dose by the conjunctival route. (scielo.br)
  • The blood culture positive bacterial isolations were further subjected to classical biotyping and molecular techniques for characterization and found as non-vaccine strains of Brucella melitensis. (thefreelibrary.com)
  • Blasco JM, Díaz R. 1993.Brucella melitensis Rev-1 vaccine as a cause of human brucellosis. (innspub.net)
  • Novel diagnostic approach on the identification of Brucella melitensis Greek endemic strains-discrimination from the vaccine strain Rev. 1 by PCR-RFLP assay. (ac.ir)
  • Progress in Brucella vaccine development. (ac.ir)
  • Brucellosis is endemic in India but no information is available on population structure and genetic diversity of Brucella spp. (hindawi.com)
  • Brucella melitensis as causative agent for neck abscess in an endemic area. (thefreedictionary.com)
  • To better understand the currently endemic B. melitensis strains in China, three Brucella genotyping methods were applied to 110 B. melitensis strains obtained in past several years. (frontiersin.org)
  • The performance of the Bactec FX blood culture system for detecting Brucella bacteremia within the routine 1-week incubation period was assessed in a prospective study conducted in an area in southern Israel in which Brucella melitensis is endemic. (asm.org)
  • Global travel and the ubiquitous importation of livestock and animal products should prompt clinicians to consider the diagnosis of brucella outside of traditional endemic regions. (ispub.com)
  • Sustained and differential antibody responses to virulence proteins of Brucella melitensis during acute and chronic infections in human brucellosis. (ebscohost.com)
  • He was initially started on intravenous vancomycin and tazobactam/piperacillin, However, cultures of blood and bursal fluid subsequently grew Brucella melitensis , and the antibody titer to brucella was positive at 1:2560. (shmabstracts.com)
  • The properties of TBG as a fluorogenic substrate for HRP and its application in a fluorometric enzyme-linked immunosensing system were compared with commercially available substrates such as p -hydroxyphenylpropionic acid (pHPPA), chavicol and Amplex red using Brucella melitensis antibody (BrAb) as a model analyte. (mdpi.com)
  • 2010. Brucella has been a significant pathogen over time as B. melitensis was recovered from a 14th century human skeleton's calcified nodule Kay et al. (samsungupdate.com)
  • Specific genes and biological processes identified in this study will further help elucidate how both host and Brucella interact during the early infectious process to the eventual benefit of the pathogen and to the detriment of the naïve host. (omicsdi.org)
  • In this work, a patient living in Shanxi Province in China who had suffered a spontaneous abortion underwent pathogen detection to analyse the genetic characteristics of the spontaneous abortion-related Brucella strain. (biomedcentral.com)
  • This study aimed at molecular detection and characterization of Brucella spp from Pakistan. (thefreelibrary.com)
  • To our knowledge, this is the first report of molecular characterization of B. melitensis isolates from Pakistan. (thefreelibrary.com)
  • Antigenic, Immunologic and Genetic Characterization of Rough Strains B.abortus RB51, B.melitensis B115 and B.melitensis B18. (ebscohost.com)
  • This characterization of B. melitensis reservoir cells could provide a better understanding of Brucella persistence in the host and lead to the design of more efficient therapeutic strategies. (duhnnae.com)
  • Brucella antigen in liver and spleen from Asian badgers infected with Brucella melitensis , northwestern China. (cdc.gov)
  • With the National Standard serum the titres to B. melitensis were consistently lower than those to B. abortus antigen. (up.ac.za)
  • Although a similar trend was seen with the field sera, there were 7/346 sera which had twofold or higher titres to B. melitensis antigen. (up.ac.za)
  • Although this may be due to the vagaries of the test it also warrants closer investigation of the animals concerned to see whether M-antigen predominant Brucella biotypes are possibly present. (up.ac.za)
  • The objective of the present study was to clone, analyze the sequence, and predict the epitopes of Omp31 gene as a major B. melitensis antigen. (ac.ir)
  • Immunity against Brucella melitensis involves antigen-specific CD4 + and CD8 + T-cells activation and humoral immune responses. (ac.ir)
  • Induction of antigen-specific Th1-type immune responses by gamma-irradiated recombinant Brucella abortus RB51. (ac.ir)
  • This study was designed to evaluate the immunogenicity of DNA vaccines encoding B. melitensis outer membrane proteins (Omp25 and Omp31) against B. melitensis Rev1 in a mouse model. (nih.gov)
  • Brucella strains express uncanonical virulence factors including lipopolysaccharides (LPS), virulence regulator proteins and phosphatidylcholine, but lack most of classical virulence factors such as invasive protease, extoxin, or virulence plasmids [ 3 ]. (ijbs.com)
  • In this study, the outer membrane proteins (Omps) of Brucella melitensis 152, 183 and 293 of local isolates were extracted and characterized using Sodium Dodecyl Sulfate-polyacrylamide Gel Electrophoresis (SDS-PAGE). (upm.edu.my)
  • 8. Cloeckaert A, Verger J-M, Grayon M, Grepinet O. Restriction site polymorphism of the genes encoding the major 25 kDa and 36 kDa outer-membrane proteins of Brucella. (ac.ir)
  • B. melitensis can be transmitted to humans through ingestion of contaminated dairy products. (wikipedia.org)
  • The mechanism of transmission was not determined until 1905, when Temi Żammit found that apparently healthy goats could infect humans with M. melitensis via their milk. (wikipedia.org)
  • Live attenuated Brucella vaccines are considered effective in animals but they may be potentially infectious to humans, so it is vital to improve the immunoprotective effects and safety of vaccines against Brucella. (nih.gov)
  • In addition, because of the low infecting dose, the high infectivity, and the ability to cause prolonged disability in humans and loss of productivity in animal husbandry, brucellae are potential candidates for biological warfare ( 3 ). (asm.org)
  • This study aimed obtaining the data via Delphi Expert Opinion Surveys (DEOS) and Targeted Group Surveys (TGS) required for calculation of financial losses caused by Brucella abortus and Brucella melitensis in infected animals and humans and also required for performing cost-benefit analysis for. (ebscohost.com)
  • Brucellosis is a widespread disease affecting livestock and humans caused by Brucella spp. (upm.edu.my)
  • Brucellosis, caused by the Brucella strains, is a zoonotic bacterial disease that affects thousands of humans and animals around the world inflicting discomfort and huge economic losses. (ac.ir)
  • The disease is caused by bacteria of the genus Brucella , a member of the α -2 Proteobacteria [ 1 ]. (hindawi.com)
  • Because brucellae are localized intracellularly, infections with these bacteria should be treated with antibiotics that can penetrate the cell at high concentrations. (asm.org)
  • Because of the slow replication time of brucellae ( 7 ), coupled with the release of only small quantities of measurable CO 2 by members of the genus ( 8 ) and the fact that the magnitude of bacteremia in adults is usually lower than that observed in children ( 9 ), it is possible that the presence of the bacteria in blood culture media cannot be revealed within the routine incubation period. (asm.org)
  • Human brucellosis, with more than 500,000 new cases, is one of the most common zoonotic infections worldwide, which is caused by Gram-negative bacteria, Brucella spp. (ijmm.org)
  • Macrophage, a kind of early defense cell against pathogenic bacteria, is primary target of Brucella [ 2 , 5 ]. (ijbs.com)
  • Brucella is a genus of Gram-negative bacteria, named after David Bruce 1855-1931. (samsungupdate.com)
  • Neither of the two tests is able to avoid the detection of false positive serological reactions (FPSRs) caused by bacteria sharing S-LPS components with Brucella spp. (researchsquare.com)
  • Brucellosis is a bacterial zoonosis of worldwide distribution caused by bacteria of the genus Brucella. (figshare.com)
  • Moreover, deletion of the Brucella formyltransferase gene wbkC in wbdR constructs generated bacteria producing only N-acetyl-perosamine homopolymers, proving that wbdR can replace for wbkC. (figshare.com)
  • However, it is well known that the virulence of B. melitensis is more than B. abortus , but there is not any strong evidence to verify it. (scialert.net)
  • For this purpose, in refer to potent antigenicity of hsps in various infectious as well as some hsp molecules act as potent activator of macrophage (danger signal), we hypothesized that difference in virulence between B. abortus and B. melitensis may be originated from difference in pattern of response to heat shock induced by high degree of fever that usually present in brucellosis. (scialert.net)
  • Until now, 61 genomes of B . melitensis have been sequenced and made available in the GenBank. (hindawi.com)
  • SNP-based phylogenetic tree of Brucella melitensis using 29 genomes available at time of assay development. (cdc.gov)
  • Spleen cells from mice infected with the rough Brucella melitensis strain B115 were fused with NSO myeloma cells. (microbiologyresearch.org)
  • These results indicate that O-chain expressed in the rough B. melitensis strain B115 is immunogenic in mice, not exposed at the cell surface but present in the cytoplasm and most probably at the cytoplasmic membrane. (microbiologyresearch.org)
  • Survey of Protection of Recombinant Cell Surface Protein 31kDa from Brucella melitensis in BALB/c Mice. (ac.ir)
  • Protection of mice against brucellosis by vaccination with Brucella melitensis WR201(16MDeltapurEK). (ac.ir)
  • We performed an in situ analysis by fluorescent microscopy of the phenotype of B. melitensis infected spleen cells from intranasally infected IL-12p40- BALB-c mice and the impact of STAT6 deficiency on this phenotype. (duhnnae.com)
  • 5] In vitro antimicrobial susceptibility testing of Brucella spp. (ijmm.org)
  • The live attenuated strains like Brucella melitensis Rev1 and B. abortus S19 and RB51 are being used to control brucellosis in domesticated animals however they have some disadvantages, such as these strains provoke antibodies to their lipopolysaccharide (LPS), making it difficult to distinguish vaccinated animals from those naturally infected. (upm.edu.my)
  • Brucellosis is an important zoonotic disease caused primarily by the bacterial pathogens Brucella melitensis and B. abortus. (ebscohost.com)
  • Bacterial persistence and immunity in goats vaccinated with a purE deletion mutant or the parental 16M strain of Brucella melitensis. (asm.org)
  • Brucella melitensis is a Gram-negative coccobacillus bacterium from the Brucellaceae family. (wikipedia.org)
  • Serology reagents intended to detect antibodies to Brucella melitensis, a coccobacillus bacterium. (ophthalmologymanagement.com)
  • In April and May 2015, two laboratory-confirmed cases of Brucella melitensis were reported, exposing dozens of health workers to the serious bacterium. (outbreaknewstoday.com)
  • MALDI-TOF MS could not identify the organism, because Brucella is not included in the instrument's reference database. (outbreaknewstoday.com)
  • The lipopolysaccharide (LPS) is considered the major virulent factor in Brucella spp. (ebscohost.com)
  • Usually, Brucella strains devoid of O-PS (rough mutants) are less virulent than the wild type. (ebscohost.com)
  • To evaluate host responses, young goats were inoculated subcutaneously with a genetic deletion mutant (deltapurE201) of Brucella melitensis (n = 6), its virulent parental strain 16M (n = 6), or saline (n = 6). (asm.org)
  • melitensis is the most virulent and pathogenic strain for both, man and animal Alton, 1990. (samsungupdate.com)
  • These results open the possibilities of developing brucellosis vaccines that are both antigenically tagged and lack the diagnostic epitopes of virulent field strains, thereby solving the diagnostic interference created by current vaccines against Brucella. (figshare.com)
  • We report an Asian badger ( Meles leucurus ) in China naturally infected with B. melitensis biotype 3. (cdc.gov)
  • MLVA-16 discerned a total of 57 MLVA-16 genotypes among these Brucella strains, with 41 genotypes being firstly detected and the other 16 genotypes being previously reported. (frontiersin.org)
  • Results were compared to MLVA typing results, the current gold-standard of Brucella typing. (uni-muenchen.de)
  • Minimum spanning tree of 221 Brucella melitensis isolates based on MLVA-10 genotyping. (cdc.gov)
  • These mAbs also reacted in ELISA with O polysaccharides (OPS) from the smooth Brucella abortus strain 99 and the smooth B. melitensis strain 16M and thus recognize epitopes present on the O-chain. (microbiologyresearch.org)
  • A total of 22 monoclonal antibodies (mAbs) were produced against Omp31 of Brucella ( B. ) melitensis , of which 13 recognized five linear epitopes, 7 reacted with semi-conformational epitopes and 2 reacted with conformational epitopes, respectively. (biomedcentral.com)
  • Selection of protective epitopes for Brucella melitensis by DNA vaccination. (ac.ir)
  • Absorption experiments with immune sera revealed that the wbdR constructs triggered antibodies to new immunogenic epitope(s) and the use of monoclonal antibodies proved that B. abortus and B. melitensis wbdR constructs respectively lacked the A or M epitopes, and the absence of the C epitope in both backgrounds. (figshare.com)
  • We transformed the plasmids in B. melitensis 16M competent cells and observed significant up/downregulation of target mRNAs. (magiran.com)
  • Therefore, to investigate the relationship between Brucella melitensis 16M and SUMO, we constructed plasmids and cells lines suitable for overexpression and knockdown of SUMO1 and Ubc9 genes. (bvsalud.org)
  • B . melitensis contains more than 3000 genes that are distributed over two circular chromosomes. (hindawi.com)
  • In particular, a total of 25 target genes are involved in regulating apoptosis and autophagy, indicating that these miRNAs may play important regulatory roles in the Brucella -host interactions. (ijbs.com)
  • The identification of two protective DNA vaccines from a panel of five plasmid constructs encoding Brucella melitensis 16M genes. (ac.ir)
  • Results: Our results showed the high presence of relE-rhh-like, Fic-Phd, Cog-rhh, and cogTcogAT s TAs genes in B. melitensis isolates that were 96.25%, 92.5%, 96.25%, and 95%, respectively. (eurekaselect.com)
  • Conclusion: A high presence of TAs genes in clinical B. melitensis isolates revealed that the TA system could be an antibacterial target in B. melitensis but more investigation is necessitated to elucidate the exact roles of these genes. (eurekaselect.com)
  • In THP-1 macrophage infected with Brucella melitensis we studied the expression levels of four host's genes, i.e. (ac.ir)
  • Our data showed that at this molecular level, except for FRMD5 that was downregulated, the other three genes were upregulated by B. melitensis. (ac.ir)
  • Hybridoma supernatants were screened in ELISA with cell walls (CW), sonicated cell extracts (CE) and rough lipopolysaccharide (R-LPS) of B. melitensis strain B115 and whole B. melitensis B115 cells. (microbiologyresearch.org)
  • An ELISA based on a R strain of Brucella, i.e. (researchsquare.com)
  • All positive sera, i.e. from Brucella-infected animals, were RBPT positive and CFT positive (ICFTU ranging from 20 to 1280) and gave ELISA O.D. values above the cutoff. (researchsquare.com)
  • Conclusions: The B. melitensis B115-based ELISA systematically unravelled all false positive (FP) sera while confirming the diagnosis in Brucella-infected animals. (researchsquare.com)
  • In a time course study, we characterized global gene expression profile of B. melitensis-infected bovine Peyer patches in the first 4 h p.i. (omicsdi.org)
  • Microarrays were used to examine the transcriptional profiles of bovine intestinal Peyer patches infected with wild type Brucella melitensis 16M across five time points (15 min, 30 min, 1, 2 and 4 hours). (omicsdi.org)
  • Brucella melitensis B115, was employed to measure specific IgG responses in a collection of bovine sera (n=648). (researchsquare.com)
  • Ovine and Caprine Brucellosis: Brucella melitensis" (PDF). (wikipedia.org)
  • In conclusion, ovine origin B. melitensis strains evaluated in this study were resistant to at least one antimicrobial (51.2%) that is commonly used in human clinical medicine against brucellosis. (yyu.edu.tr)
  • Brucella invades and replicates in professional phagocytic cells such as macrophages and dendritic cells as well as nonprofessional phagocytes such as trophoblasts [ 3 - 5 ]. (hindawi.com)
  • Previous studies revealed the mRNA expression profiles of macrophages infected by Brucella based on microarray [ 10 , 11 ]. (ijbs.com)
  • In macrophages, Brucella 16M-dependent apoptosis and immune factors were induced by SUMO1/Ubc9 overexpression and restricted by SUMO1/Ubc9 depletion. (bvsalud.org)
  • We noted no effect on the expressions of SUMO1 and Ubc9 in B. melitensis 16M lipopolysaccharide-prestimulated mouse RAW264.7 macrophages. (bvsalud.org)
  • Brucella melitensis and Mycobacterium tuberculosis depict overlapping gene expression patterns induced in infected THP-1 macrophages', Iranian Journal of Veterinary Research , 16(4), pp. 368-373. (ac.ir)
  • In Iran, approximately, 238.6 cases/million population of human brucellosis (overwhelmingly due to Brucella melitensis) are reported annually. (ijmm.org)
  • The isolated aptamers were able to identify B. melitensis and B. abortus with a remarkable binding efficiency and appropriated K d in a picoMolar range and therefore can be good candidates in the development of any rapid assay test implanted on routine brucellosis diagnoses. (ac.ir)
  • Methods: We evaluated the presence of different toxin-antitoxin systems, including relE- rhhlike, Fic- Phd, Cog- Rhh, and cogT- cogAT in 40 clinical Brucella melitensis isolates using PCRbased sequencing assay. (eurekaselect.com)
  • Although brucellar infections can be ascertained with serological tests and nucleic acid amplification assays, culture detection of circulating Brucella organisms remains a diagnostic cornerstone. (asm.org)
  • However, since 2014 a significant increase of imported infections caused by Brucella (B.) melitensis has been noticed in Germany. (uni-muenchen.de)
  • The solubility, cytotoxicity, and efficacy of these nano-packed antibiotics on Brucella -infected murine phagocytic cells were examined, as compared with free antibiotics. (ac.ir)
  • however, the efficacy of rifampicin on Brucella -infected murine phagocytic cells was enhanced. (ac.ir)
  • In this study, we generated and characterized 22 novel murine monoclonal antibodies (mAbs) binding native Omp31 of B. melitensis . (biomedcentral.com)
  • Brucella is a monospecific genus Brucella melitensis with multiple biovars. (samsungupdate.com)
  • Antimicrobial susceptibility tests are not yet standardized for brucellae, and routine susceptibility tests cannot be performed in microbiology laboratories. (asm.org)
  • Strains identified as B. melitensis were stored in brucella broth (Remel Inc.) at −20°C until susceptibility testing. (asm.org)
  • Antimicrobial susceptibility testing of the Brucella isolates was performed by two different techniques: E test and the checkerboard method. (asm.org)
  • The aim of this study was to assess the susceptibility of Brucella isolates to common antibiotics that are prescribed by the physician for the treatment of brucellosis and also to determine the minimum inhibitory concentration 50% (MIC 50 ) and MIC 90 for these antibiotics. (ijmm.org)
  • Although all the Brucella isolates were susceptible, antimicrobial susceptibility test should be recommended in patients with recurrent brucellosis or life-threatening organ involvement. (ijmm.org)
  • L'objectif de cette étude est le clonage, l'analyse et le séquençage du gène Omp31 ainsi que la prédiction des épitopes de l'antigène majeur de B. melitensis. (ac.ir)
  • A chimeric gene encoding trigger factor (TF), Omp31 48-74 and BP26 87-111 fragments (TOB) from B. melitensis was successfully cloned, expressed in Escherichia coli BL21-DE3 and purified by Ni-NTA agarose column. (ac.ir)
  • Agglutination test for Brucella was positive at 1:2560 and Brucella IgG and IgM tests were also positive. (ispub.com)
  • We tested putative Brucella colonies by using H 2 S production, dye inhibition, agglutination by monospecific serum, and sensitivity to bacteriophages ( Appendix Table). (cdc.gov)
  • Gram-negative, non-spore-forming, nonmotile coccobacilli which were oxidase and urease positive were tested for agglutination with monospecific anti- Brucella serum (Remel Inc., Lenexa, Kans. (asm.org)
  • Antibodies to recombinant TOB (rTOB) have been investigated in Brucella-infected human sera and a pool serum prepared from B. melitensis -vaccinated rabbits. (ac.ir)
  • Results of agglutination tests for Brucella in serum and CSF were positive for all patients, although titres were less in the CSF. (nih.gov)
  • We present a rare case of brucella endocarditis, forming a vegetation on the mitral valve. (ebscohost.com)
  • Succesful management of Brucella melitensis endocarditis with combined medical and surgical approach. (thefreedictionary.com)
  • In all published studies on Brucella infective endocarditis with a cardiovascular implantable device, the recommendation was for device removal and extended treatment with doxycycline combined with rifampin and/or trimethoprim-sulfamethoxazole, plus parenteral aminoglycosides for the first 3 weeks. (dovepress.com)
  • Furthermore, we constructed a novel shuttle expression plasmid for gene expression, enabling stable replication in B. melitensis. (magiran.com)
  • The gene sequence of B. melitensis Rev 1 strain was submitted to NCBI database. (ac.ir)
  • We report that the Brucella O-PS can be structurally and antigenically modified using wbdR, the acetyl-transferase gene involved in N-acetyl-perosamine synthesis in Escherichia coli O157:H7. (figshare.com)
  • Brucellosis is a worldwide zoonotic disease caused by Brucella spp. (frontiersin.org)
  • Sixteen strains of B. melitensis isolated from blood cultures at the Microbiology Laboratory of Gaziantep University from January through December 2003 were included in this study. (asm.org)
  • It is concluded that the Bactec FX system is able to detect the vast majority of episodes of Brucella bacteremia within the 1-week incubation protocol instituted in most clinical microbiology laboratories and without the need to perform blind subcultures of negative vials, enabling early diagnosis and saving labor and incubation time and space. (asm.org)
  • Recent studies, however, have demonstrated that automated blood culture systems based on monitoring of CO 2 production have improved sensitivity, compared to manual methods, enabling detection of brucellae in pediatric patients within the customary 1-week incubation protocol followed in most clinical microbiology laboratories ( 6 ). (asm.org)
  • Brucellosis is an important zoonosis caused by Brucella spp. (sigmaaldrich.com)
  • Brucella melitensis causes a worldwide zoonosis. (innspub.net)
  • Goat farm management and Brucella serological test among goat keepers and livestock officers, 2011-2012, Nakhon Si Thammarat Province, southern Thailand. (springer.com)
  • The aim of this study was to evaluate the rate of agreement of two in vitro tests for synergy, E test and the checkerboard method, with various combinations of antibiotics that penetrate the cell wall against Brucella melitensis isolates recovered from blood. (asm.org)
  • Because of the fastidious nature of members of the genus Brucella , prolonged incubation of media and performance of blind subcultures of negative blood culture vials have been traditionally recommended to maximize isolation of these organisms ( 4 , 5 ). (asm.org)
  • Proteinase K LPS preparations from B. melitensis B115 analysed by immunoblotting with one mAb (12G12) recognizing S-LPS of both A and M specificity displayed the typical S-LPS high-molecular-mass ladder pattern but no S-LPS was detected in the phenol/water/chloroform/light petroleum LPS preparation of the same strain. (microbiologyresearch.org)
  • mAb 12G12, specific for S-LPS, and a mAb (A68/03F03/D05) specific for R-LPS were used to localize the O-chain and R-LPS expressed in B. melitensis strain B115 by immunoelectron microscopy. (microbiologyresearch.org)
  • Immunogold labelling was observed at the surface of B. melitensis B115 cells with the anti-R-LPS mAb but not with the anti-S-LPS mAb. (microbiologyresearch.org)
  • Brucella melitensis has been isolated from wildlife, such as chamois ( Rupicapra rupicapra ) ( 2 ), Alpine ibex ( Capra ibex ) ( 3 ), and Iberian wild goat ( Capra pyrenaica ) ( 4 ). (cdc.gov)
  • Case-control study on risk factors associated with Brucella melitensis in goat farms in Peninsular Malaysia. (springer.com)
  • In 1887, Micrococcus melitensis was isolated in Malta by David Bruce from the spleen of a soldier who had died from acute brucellosis. (wikipedia.org)
  • The 43 B. melitensis isolates were collected between 1991 and 1994 from blood or bone marrow cultures of individual inpatients with acute brucellosis at Hacettepe University Hospital. (asm.org)
  • Forty-eight Brucella strains were collected from patients with acute brucellosis. (ijmm.org)
  • We used Brucella reference strains ( B. melitensis 16M and B. abortus 2308) as positive controls and double-distilled water as a negative control. (cdc.gov)