A species of the genus BRUCELLA whose natural hosts are cattle and other bovidae. Abortion and placentitis are frequently produced in the pregnant animal. Other mammals, including humans, may be infected.
A genus of gram-negative, aerobic bacteria that causes BRUCELLOSIS. Its cells are nonmotile coccobacilli and are animal parasites and pathogens. The bacterium is transmissible to humans through contact with infected dairy products or tissue.
Infection caused by bacteria of the genus BRUCELLA mainly involving the MONONUCLEAR PHAGOCYTE SYSTEM. This condition is characterized by fever, weakness, malaise, and weight loss.
A disease of cattle caused by bacteria of the genus BRUCELLA leading to abortion in late pregnancy. BRUCELLA ABORTUS is the primary infective agent.
A bacterial vaccine for the prevention of brucellosis in man and animal. Brucella abortus vaccine is used for the immunization of cattle, sheep, and goats.
A species of the genus BRUCELLA whose natural hosts are sheep and goats. Other mammals, including humans, may be infected. In general, these organisms tend to be more virulent for laboratory animals than BRUCELLA ABORTUS and may cause fatal infections.
A species of the genus BRUCELLA which are pathogenic to SHEEP.
A species of gram-negative bacteria infecting DOGS, the natural hosts, and causing canine BRUCELLOSIS. It can also cause a mild infection in humans.
Premature expulsion of the FETUS in animals.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (From Stedman, 26th ed)
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
A four-carbon sugar that is found in algae, fungi, and lichens. It is twice as sweet as sucrose and can be used as a coronary vasodilator.
A genus of the family CHLAMYDIACEAE comprising gram-negative non CHLAMYDIA TRACHOMATIS-like species infecting vertebrates. Chlamydophila do not produce detectable quantities of glycogen. The type species is CHLAMYDOPHILA PSITTACI.
A genus of the family Bovidae having two species: B. bison and B. bonasus. This concept is differentiated from BUFFALOES, which refers to Bubalus arnee and Syncerus caffer.
Substances elaborated by bacteria that have antigenic activity.
A species of gram-negative bacteria, primarily infecting SWINE, but it can also infect humans, DOGS, and HARES.
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
A species of gram-negative, obligately aerobic rods. Motility occurs by peritrichous flagella. (From Bergey's Manual of Determinative Bacteriology, 9th ed)
Suspensions of attenuated or killed bacteria administered for the prevention or treatment of infectious bacterial disease.
Proteins found in any species of bacterium.
The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.
An encapsulated lymphatic organ through which venous blood filters.
A bright bluish pink compound that has been used as a dye, biological stain, and diagnostic aid.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
Ruminants of the family Bovidae consisting of Bubalus arnee and Syncerus caffer. This concept is differentiated from BISON, which refers to Bison bison and Bison bonasus.
Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed)
Proteins isolated from the outer membrane of Gram-negative bacteria.
Administration of vaccines to stimulate the host's immune response. This includes any preparation intended for active immunological prophylaxis.
Diseases of domestic and mountain sheep of the genus Ovis.
Benzoate derivatives substituted by one or more hydroxy groups in any position on the benzene ring.
Any of numerous agile, hollow-horned RUMINANTS of the genus Capra, in the family Bovidae, closely related to the SHEEP.
Infections resulting from the implantation of prosthetic devices. The infections may be acquired from intraoperative contamination (early) or hematogenously acquired from other sites (late).
Prostheses used to partially or totally replace a human or animal joint. (from UMDNS, 1999)
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
A publication issued at stated, more or less regular, intervals.
Enzymes that catalyze the cleavage of a carbon-carbon bond of a 3-hydroxy acid. (Dorland, 28th ed) EC 4.1.3.
A species of gram-negative bacteria pathogenic to CHICKENS; TURKEYS, and guinea fowls. It causes disease in a wide variety of organs and tissues including JOINTS, tendon sheaths and the RESPIRATORY TRACT.
Rupture of bacterial cells due to mechanical force, chemical action, or the lytic growth of BACTERIOPHAGES.
Serum that contains antibodies. It is obtained from an animal that has been immunized either by ANTIGEN injection or infection with microorganisms containing the antigen.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
A genus of obligately anaerobic ARCHAEA, in the family THERMOPROTEACEAE. They are found in acidic hot springs and water holes.
Cellular processes in biosynthesis (anabolism) and degradation (catabolism) of CARBOHYDRATES.
The genetic complement of a BACTERIA as represented in its DNA.
A major C19 steroid produced by the ADRENAL CORTEX. It is also produced in small quantities in the TESTIS and the OVARY. Dehydroepiandrosterone (DHEA) can be converted to TESTOSTERONE; ANDROSTENEDIONE; ESTRADIOL; and ESTRONE. Most of DHEA is sulfated (DEHYDROEPIANDROSTERONE SULFATE) before secretion.
Bone-forming cells which secrete an EXTRACELLULAR MATRIX. HYDROXYAPATITE crystals are then deposited into the matrix to form bone.
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.

Modulation of endocytosis in nuclear factor IL-6(-/-) macrophages is responsible for a high susceptibility to intracellular bacterial infection. (1/780)

Activated macrophages kill bacteria, a function known to depend on the expression of NF-IL-6. Here, it is demonstrated that the attenuated Brucella abortus vaccine strain 19 replicates much better in NF-IL-6-/- than in NF-IL-6(+/+) and NF-IL-6(+/+)-activated murine macrophages and at levels comparable to those observed in normal macrophages infected with the pathogenic strain 2308. The role of NF-IL-6 in the inhibition of intracellular bacterial replication is related to its control of endocytosis and membrane fusion between endosomes and Brucella-containing phagosomes. Addition of the granulocyte-CSF (G-CSF), whose induction is impaired in NF-IL-6(-/-) macrophages, restores both endocytosis and the morphology of endosomes, together with bactericidal activity. Regulation of membrane traffic in endocytosis by G-CSF whose expression is controlled by NF-IL-6 may explain how a host cell can control intracellular bacterial replication.  (+info)

Immunity to Brucella in mice vaccinated with a fraction (F8) or a killed vaccine (H38) with or without adjuvant. Level and duration of immunity in relation to dose of vaccine, recall injection and age of mice. (2/780)

Immunity to Brucella in the mouse, assessed by bacterial spleen counts 15 days after intraperitoneal inoculation of a standard challenge of B. abortus 544, has been studied with two vaccines, one experimental, composed of a fraction of the bacterial cell-wall (F8) extracted from B. abortus 99, the other of killed whole bacteria, B. melitensis 53 H38, taken as reference (H38). The level of primary immunity depended on the dose of vaccine, the presence of oil adjuvant and the age of the mouse. The presence of adjuvant enabled the immunization to F8 to continue beyond the first month, to reach its maximum around the fourth month, and to remain stable for at least 7 months. A booster injection 3 or 6 months after the primary vaccination reinforced existing immunity but did not increase it beyond a certain level. The effect of the recall injection was clearly demonstrated with low doses which gave a lower level of primary immunity.  (+info)

Murine macrophage-lymphocyte interactions: scanning electron microscopic study. (3/780)

Light and scanning electron microscopic observations revealed murine macrophage-lymphocyte interactions involving the initial contact of peritoneal, spleen, or thymus lymphocytes with peritoneal macrophage processes or microprocesses followed by clustering of lymphocytes over the central nuclear area of the macrophages. Lymphocyte-lymphocyte clustering was not observed in the absence of macrophages. Attachment and subsequent clustering appeared not to require the presence of serum or antigen; the attachment of allogeneic or xenogeneic lymphocytes was comparable to that seen in the syngeneic system, but central clustering of these lymphocytes failed to occur. No attachment or clustering was observed when thymic lymphocytes were cultured with thymus derived fibroblasts rather than with peritoneal macrophages. Lymphocyte attachment to immune, antigen-activated, syngeneic macrophages occurred more rapidly than that to normal unstimulated syngeneic macrophages; however, lymphocytes attached to the "activated" macrophages appeared to be killed by a nonphagocytic mechanism. A similar increase in the rate of lymphocyte attachment to macrophages occurred in the presence of migration inhibitory factor. Subsequent lymphocyte clustering on macrophages was observed in the migration inhibitory factor-stimulated cultures. In addition, lymphocyte-macrophage interactions similar to those in vitro were observed to occur in vivo on intraperitoneally implanted cover slips.  (+info)

Plaque-forming cells in mice after experimental infection with Brucella abortus. (4/780)

Cells producing antibody to brucella lipopolysaccharide were detected in spleens of mice infected with Brucella abortus 19 by a hemolytic plaque assay. The appearance of immunoglobulin M-producing cells preceded humoral antibodies. The primary plaques were observed 5 days after inoculation, and they were still present by day 70.  (+info)

Antibody response to antigens distinct from smooth lipopolysaccharide complex in Brucella infection. (5/780)

The smooth lipopolysaccharide complex of the outer surface of smooth Brucella abortus cells is believed to be the antigenic component involved in serological tests routinely used for the diagnosis of brucellosis. Sera from cattle vaccinated or infected with B. abortus generally contain antibody directed toward the smooth lipopolysaccharide complex. The brucella organism contains a large number of other antigenically distinct components. The biological significance of some of these antigens has been demonstrated by showing that sera from infected cattle have precipitins to these components. These sera revealed up to seven distinct lines in immunoelectrophoresis with a protein-rich antigen mixture prepared from rough strain B. abortus 45/20, whereas sera from strain 19-vaccinated cattle did not reveal these lines at 4 or more months after vaccination. Monospecific antisera were prepared against six antigens in this mixture, and the purification of two of them by antibody affinity chromatography is described.  (+info)

The siderophore 2,3-dihydroxybenzoic acid is not required for virulence of Brucella abortus in BALB/c mice. (6/780)

2,3-Dihydroxybenzoic acid (DHBA) is the only siderophore described for Brucella, and previous studies suggested that DHBA might contribute to the capacity of these organisms to persist in host macrophages. Employing an isogenic siderophore mutant (DeltaentC) constructed from virulent Brucella abortus 2308, however, we found that production of DHBA is not required for replication in cultured murine macrophages or for the establishment and maintenance of chronic infection in the BALB/c mouse model.  (+info)

Structure and function prediction of the Brucella abortus P39 protein by comparative modeling with marginal sequence similarities. (7/780)

A methodology is proposed to solve a difficult modeling problem related to the recently sequenced P39 protein. This sequence shares no similarity with any known 3D structure, but a fold is proposed by several threading tools. The difficulty in aligning the target sequence on one of the proposed template structures is overcome by combining the results of several available prediction methods and by refining a rational consensus between them. In silico validation of the obtained model and a preliminary cross-check with experimental features allow us to state that this borderline prediction is at least reasonable. This model raises relevant hypotheses on the main structural features of the protein and allows the design of site-directed mutations. Knowing the genetic context of the P39 reading frame, we are now able to suggest a function for the P39 protein: it would act as a periplasmic substrate-binding protein.  (+info)

Comparison of sensitivity and specificity of purified lymphocyte and whole-blood in vitro lymphocyte stimulation assays in detection of Brucella abortus infection in cattle. (8/780)

A study was conducted to compare the sensitivity and specificity of purified lymphocyte and whole-blood in vitro lymphocyte stimulation assays in detection of Brucella abortus infection in cattle. Cattle used were infected with B. abortus field strains or strain 19. Peripheral blood was collected, and lymphocytes for the technique. The blood for the whole-blood lymphocyte stimulation assay was diluted 10-fold with RPMI 1640 medium (without additional serum supplement) and cultured. The two tests were run simultaneously, and B. abortus soluble antigen or concanavalin A was added to the cultures. The cultures were incubated for 6 days and assayed for [3H] thymidine incorporation into their DNA. Generally, cultures of the purified lymphocyte stimulation assay had higher counts per minute than those of the whole-blood lymphocyte stimulation assay, but the stimulation ratios for the two tests were comparable. The two assays were comparable in terms of their sensitivity and specificity as applied to detection of brucella infection in cattle.  (+info)

The Brucella abortus strain S19, a spontaneously attenuated strain, has been used as a vaccine strain in vaccination of cattle against brucellosis for six decades. Despite many studies, the physiological and molecular mechanisms causing the attenuation are not known. We have applied pyrosequencing technology together with conventional sequencing to rapidly and comprehensively determine the complete genome sequence of the attenuated Brucella abortus vaccine strain S19. The main goal of this study is to identify candidate virulence genes by systematic comparative analysis of the attenuated strain with the published genome sequences of two virulent and closely related strains of B. abortus, 9-941 and 2308. The two S19 chromosomes are 2,122,487 and 1,161,449 bp in length. A total of 3062 genes were identified and annotated. Pairwise and reciprocal genome comparisons resulted in a total of 263 genes that were non-identical between the S19 genome and any of the two virulent strains. Amongst these, 45 genes
Brucella abortus is a facultative intracellular bacterial pathogen, which causes abortion in cattle and undulant fever in human. B. abortus strain RB51 (Strain RB51) is the official vaccine for bovine brucellosis in the USA. B. abortus strain RB51 can be used as a vector for the over-expression of its own (homologous) as well as heterologous protective antigens. The immune system can detect these heterologous antigens and produce a response. Expressing a protein in different bacterial compartments has been shown to affect its accessibility to the immune system and the way the antigen is processed by antigen presenting cells. In order to determine if the immune response is affected by the localization of the antigen, green fluorescent protein (GFP) was expressed at three different locations in B. abortus strain RB51, outer-membrane (OM), periplasmic space (PS) and in the cytoplasmic region (CR) of B. abortus strain RB51. This localization was obtained by transforming strain RB51 with plasmids ...
Abstract. We report the draft genome sequences of two Brucella abortus strains LMN1 and LMN2 isolated from cattle and pig. The LMN1 and LMN2 have the genome size of 3,395,952 bp and 3,334,792 bp, respectively. In addition to the conserved genes of Brucella, few novel regions showing similarity to the phages were identified in both strains.. Keywords: genome sequence, Brucella abortus ...
Brucella abortus induces an inflammatory response that stimulates the endocrine system resulting in the secretion of cortisol and dehydroepiandrosterone (DHEA). Osteoarticular brucellosis is the most common presentation of the active disease in humans and we have previously demonstrated that B. abortus infection inhibits osteoblast function. We aimed to evaluate the role of cortisol and DHEA on osteoblast during B. abortus infection. B. abortus infection induces apoptosis and inhibits osteoblast function. DHEA treatment reversed the effect of B. abortus infection on osteoblast by increasing their proliferation, inhibiting osteoblast apoptosis and reversing the inhibitory effect of B. abortus on osteoblast differentiation and function. In contrast, cortisol increased the effect of B. abortus infection. Cortisol regulates target genes by binding to the glucocorticoid receptor (GR). B. abortus infection inhibited GR-α expression. Cells responses to cortisol not only depend on GR expression but also on its
Serological test is one of diagnostic method to detect pathogenicity brucella. Several methods are being improving such as Rose Bengal Test (RBT), Complement Fixation Test (CFT) dan Enzyme-linked Immunosorbent Assay (ELISA). Immunostick has an accuracy equivalent to ELISA and is easy to apply in the field so it is possible to be applied as a rapid test for brucellosis detection. The study aim was to know sensitivity and specificity of immunostick that were used to detecte antibody Brucella abortus using commercial antigens of B. abortus Strain 19 (S19) and B. abortus Strain 99 (S99). The test have compared with ELISA. The tests were conducted in two stages, namely (i) immunostick ability to detect antibodies in seropositive and seronegative serum, and (ii) the immunostick result were compared with ELISA result in serum grup that were be know and unknown status. A total of 250 serums were examined and result indicated that immunostick can be detect B. abortus antibodies in cattle serum with ...
Brucella abortus is a Gram-negative, facultative intracellular bacterium that causes brucellosis, a worldwide zoonotic disease leading to undulant fever in humans and abortion in cattle. The immune response against this bacterium relies on the recognition of microbial pathogen-associated molecular patterns, such as lipoproteins, lipopolysaccharides and DNA; however, the immunostimulatory potential of B. abortus RNA remains to be elucidated. Here, we show that dendritic cells (DCs) produce significant amounts of IL-12, IL-6 and IP-10/CXCL10, when stimulated with purified B. abortus RNA. IL-12 secretion by DCs stimulated with RNA depends on TLR7 while IL-6 depends on TLR7 and partially on TLR3. Further, only TLR7 plays a role in IL-12 production induced by B. abortus infection. Moreover, cytokine production in DCs infected with B. abortus or stimulated with bacterial RNA was reduced upon pretreatment with MAPK/NF-κB inhibitors. By confocal microscopy, we demonstrated that TLR7 is colocalized with B.
Dendogram of global Brucella abortus biovar 1 genotypes. Dendogram based on MLVA-16 genotyping showing the relationship of 49 B. abortus biovar 1 strains origin
Immunization of rabbits with concentrated Brucella abortus antigens for 10 weeks led to nearly total loss of serum bactericidal activity for this organism. β and γ globulin fractions of hyperimmune serum obtained by starch block electrophoresis had bactericidal activity. Sephadex G-200 gel filtration of the β globulin fraction obtained by starch block electrophoresis yielded a fraction consisting of IgM and another containing IgG and IgA; both had bacteriolytic activity. The latter subfraction was separated by column chromatography on DEAE-Sephadex into IgG and IgA (probably contaminated by 15% to 20% IgG). Bacteriolytic tests indicated that IgA had a prozone of inhibition of bacteriolysis, whereas IgM and IgG did not. Addition of excess IgA inhibited bacteriolysis produced by IgG and IgM. Bactericidal kinetics disclosed little kill by whole hyperimmune serum. Hyperimmune IgM and IgG killed Br, abortus rapidly but IgA fractions gave a lag of more than 3 min before any kill was measured. ...
PHE1 is a htrA cycL double gene deletion mutant of virulent Brucella abortus strain 2308 (S2308) which has previously been evaluated in the murine and caprine models of bovine brucellosis. This report describes the results of studies conducted with this mutant in the natural bovine host. Six sexually mature, non-gravid heifers were inoculated via the conjunctival sac with 1x1010 colony forming units (CFU) of either the parental S2308 or the htrA cycL gene deletion mutant, PHE1. At 4, 7 and 11 days post-inoculation, PHE1 was found to colonize the bovine host at lower levels than S2308. In a second experiment, eight heifers in mid-gestation were infected with 1x107CFU of either strain via the conjunctival sac. The virulent S2308 caused abortions or weak calves in 4/4 cows, while all four cows infected with PHE1 had healthy calves. Furthermore, PHE1 exhibited decreased resistance to killing by cultured bovine neutrophils and macrophages compared to the parental strain. These studies demonstrate that the B.
Brucella abortus is a facultative intracellular bacterial pathogen, which causes abortion in cattle and undulant fever in human. B. abortus strain RB51 (Strain RB51) is the official vaccine for bovine brucellosis in the USA. B. abortus strain RB51 can be used as a vector for the over-expression of its own (homologous) as well as heterologous protective antigens. The immune system can detect these heterologous antigens and produce a response. Expressing a protein in different bacterial compartments has been shown to affect its accessibility to the immune system and the way the antigen is processed by antigen presenting cells. In order to determine if the immune response is affected by the localization of the antigen, green fluorescent protein (GFP) was expressed at three different locations in B. abortus strain RB51, outer-membrane (OM), periplasmic space (PS) and in the cytoplasmic region (CR) of B. abortus strain RB51. This localization was obtained by transforming strain RB51 with plasmids ...
La mayoría de las infecciones bacterianas inducen la activación de los neutrófilos polimorfonucleares (PMN), mejoran su función microbicida y promueven la supervivencia de estos leucocitos durante períodos prolongados. Brucella abortus es un patógeno sigiloso que evade la inmunidad innata, apenas activa los PMN y resiste los mecanismos de destrucción de estos fagocitos. Los intrigantes signos clínicos que se observan durante la brucelosis son el bajo número de PMN infectados por Brucella en los órganos diana y la neutropenia en una proporción de los pacientes; características que merecen una mayor atención. Aquí demostramos que B.abortus mata prematuramente a los PMN humanos de forma dosis dependiente y específica de la célula. La muerte de los PMNs es concomitante con la liberación de lipopolisacáridos intracelulares de Brucella (Br-LPS) en vacuolas. Esta molécula y su lípido A reproducen la muerte celular prematura de los PMN, un fenómeno asociado a la baja producción de ...
Brucella causes a chronic and debilitating infection that leads to great economic losses and a public health burden. In this study, we demonstrated the brucellacidal effect of heat shock mediated by the induction of pro-inflammatory cytokines, reactive oxygen species (ROS) accumulation and apoptosis in murine macrophages and in mice. RAW264.7 cells were incubated at 43 °C, and BALB/c mice were subjected to whole body hyperthermia. The data showed a reduction in bacterial survival in the mice after daily heat exposure. This was accompanied by increased levels of cytokines TNF, IL-6, IL-1β and IFN-γ in the sera of the mice. Gene expression of NF-κB and inducible nitric oxide production were also induced in the mouse splenic cells. In parallel with the bacterial reduction in the mouse model, an increased bactericidal effect was observed in RAW264.7 cells after exposure to heat stress. In addition, the heat stress increased both the nuclear translocation of NF-κB and the expression of the heat shock
Brucella abortus is a Gram-negative bacterium that causes abortion and infertility in food animals and a chronic debilitating febrile disease in humans known as brucellosis. As with all pathogenic bacteria, the Brucella spp. require sufficient metal nutrition during the course of an infection. Host-mediated metal withdrawal defenses actively restrict the bioavailability of metals which requires invading bacteria to employ high affinity metal acquisition systems to overcome these metal-limiting conditions. While obtaining sufficient metals during host infection is critical to the survival of these bacteria, avoiding metal toxicity is equally important. Excess accumulation of one metal relative to others can lead to protein mis-metallation when surplus metal ions outcompete other metal species for their native binding sites. To prevent metal toxicity, bacteria respond to high intracellular metal concentrations by means of metal-responsive transcriptional regulators that downregulate metal import ...
Background: Lipopolysaccharides (LPSs) and several antigenic proteins of Brucella have been considered for preparation of diagnostic reagents and subunit vaccines. The objective of this study was to identify and compare immunogens of B. abortus S19 which induce humoral immune responses in human, goat and rabbit. Material and Methods: The bacterial whole cell extract was prepared in extraction buffer and resolved using two-dimensional gel electrophoresis (2-DE). The resolved antigens were reacted against human, goat and rabbit sera using western blotting. Results: At least 19, 14 and 16 immunogenic proteins were recognized in western blotting with human, goat and rabbit sera, respectively. The most abundant proteins of the bacterium with immunogenic properties in goat and rabbit but not in human, were a group of 5-6 proteins with molecular masses of 32-34 KDa and isoelectric point (pI) ranging from 4.5 to 5.7. In contrast, a group of 5 proteins with molecular weight of 45 KDa and pI in the range of 4.5
Major histocompatibility complex class I and II expression on macrophages containing a virulent strain of Brucella abortus measured using green fluorescent protein-expressing brucellae and flow cytometry ...
A serological survey of Brucella abortus in cattle originating from communal grazing areas of Kwa Zulu Natal was carried out between March 2001 and December 2003. The survey was designed as a 2-stage survey, considering the diptank as the primary sampling unit. In total 46 025 animals from 446 diptanks of 33 magisterial districts were sampled and tested using the Rose Bengal test and Complement Fixation Test. The apparent prevalence at district level was adjusted for clustering, diagnostic test sensitivity and specificity, and mapped using ArcView version 3.3. The prevalence of brucellosis in communal grazing areas of Kwa-Zulu Natal was found to be 1.45 % (0.84-2.21 %) and varied from 0 to 15.6% between magisterial districts. In 19 of the 33 magisterial districts no serological reactors were observed. A large variation in prevalence was found within diptank areas. Brucellosis was found to be most prevalent in the northeastern area of the province. The findings of the survey are discussed ...
Brucellosis is a zoonotic bacterial disease caused by Brucella spp. The virulence of these bacteria is dependent on their ability to invade and replicate within host cells. In a previous study, a putative gene bab_RS27735 encoding an amino acid ABC transporter substrate-binding protein homologous to AapJ protein was found to be involved in Brucella abortus virulence. In this study, we successfully constructed a bab_RS27735 deletion mutant, Δ27735. Compared with the wild-type strain, the lipopolysaccharide pattern of the mutant was not changed, but the growth ability was slightly defected in the exponential phase. In tolerance tests, sensitivity of the Δ27735 mutant to oxidative stress, bactericidal peptides or low pH was not different from that of the wild-type strain. Cell infection assay showed that the mutant was reduced survival within macrophages but could efficiently escape lysosome degradation. The results of a virulence test showed that the Δ27735 mutant was attenuated in a mouse model at the
In this study, we explore the regulatory roles of pro-inflammatory cytokine tumor necrosis factor alpha (TNF) in the innate immunity of macrophages against B. abortus infection. We show that infection of macrophage with B. abortus induces marked expression and secretion of TNF which subsequently binds to TNF receptor 1 (TNFR-1) and activates a downstream signaling cascade of the innate immunity. Blocking of TNF signaling resulted in a notable increase of B. abortus survival which was associated with an increase of anti-inflammatory cytokine interleukin 10 (IL-10), a beneficial effector of Brucella survival, as well as remarkable decrease of reactive oxygen species (ROS) and nitric oxide (NO), antibrucella molecules ...
Converts alpha-N-acetylneuranimic acid (Neu5Ac) to the beta-anomer, accelerating the equilibrium between the alpha- and beta-anomers. Probably facilitates sialidase-negative bacteria to compete sucessfully for limited amounts of extracellular Neu5Ac, which is likely taken up in the beta-anomer. In addition, the rapid removal of sialic acid from solution might be advantageous to the bacterium to damp down host responses.
Entry of the facultative intracellular pathogen Brucella into host cells results in the formation of endosomal Brucella-containing vacuoles (eBCVs) that initially traffic along the endocytic pathway. eBCV acidification triggers the expression of a type IV secretion system that translocates bacterial effector proteins into host cells. This interferes with lysosomal fusion of eBCVs and supports their maturation to replicative Brucella-containing vacuoles (rBCVs). Bacteria replicate in rBCVs to large numbers, eventually occupying most of the cytoplasmic volume. As rBCV membranes tightly wrap each individual bacterium, they are constantly being expanded and remodeled during exponential bacterial growth. rBCVs are known to carry endoplasmic reticulum (ER) markers, however, the relationship of the vacuole to the genuine ER has remained elusive. We have reconstructed the 3-dimensional ultrastructure of rBCVs and associated ER by correlative structured illumination microscopy (SIM) and focused ion ...
We report a case of Brucella abortus-induced brucellosis that displayed high hepatic transaminases similar to that in acute hepatitis, fourth month relapsed following doxycycline-streptomycin treatment, and developed doxycycline-related hepatotoxicity in second treatment. Brucella hepatitis may occur with various Brucella spp. and may relapse even following ...
Ubiquinone (UQ), also called coenzyme Q, and plastoquinone (PQ) are electron carriers in oxidative phosphorylation and photosynthesis, respectively. The quinoid nucleus of ubiquinone is derived from the shikimate pathway; 4-hydroxybenzoate is directly formed from chorismate in bacteria, while it can be formed from either chorismate or tyrosine in yeast. The following biosynthesis of terpenoid moiety involves reactions of prenylation, decarboxylation, and three hydroxylations alternating with three methylations. The order of these reactions are somewhat different between bacteria and yeast. Phylloquinone (vitamin K1), menaquinone (vitamin K2), and tocopherol (vitamin E) are fat-soluble vitamins. Phylloquinone is a compound present in all photosynthetic plants serving as a cofactor for photosystem I-mediated electron transport. Menaquinone is an obligatory component of the electron-transfer pathway in bacteria ...
In this study, we isolated and characterized B. abortusRA1, a transposon-derived rough mutant of standard strain 2308. We further identified the gene disrupted by Tn5 in strain RA1 as wboA, which encodes a putative glycosyltransferase, an enzyme which appears to be essential for the biosynthesis of the O side chain in B. abortus. LPS analysis of strains RA1 and RB51 indicated that both strains lack the O side chain. However, the reason for the presence of the intermediate LPS band in strain RA1 that is lacking in strain RB51 is not known (Fig. 1). One possibility is that the mutation in strain RA1 results in exposure of a glycose that can be glycosylated by glycosyltransferases that are not able to attach specific sugars in the parent or in strain RB51. The result may be a side branch in the core, generating a new glycoform. Evidence for this is the relatively high amount of galactose present in RA1, whereas only a trace amount of this glycose is present in RB51. Unfortunately, the nature of the ...
Brucella abortus causes brucellosis mainly in cattle. The infection is transmitted to humans by ingestion of animal products or direct contact with infected material. While the intracellular lifestyle of Brucella is well characterized, its extracellular survival is poorly understood. In nature, bacterial persistence is associated with biofilms, where aggregated cells are protected from adversity. The inability of Brucella abortus to aggregate under aerobiosis and that fact that the replicative niche of Brucella is characterized by microaerobic conditions prompted us to investigate the capacity of this pathogen to aggregate and grow in biofilms under microaerobiotic conditions. The results show that B. abortus aggregates and produces biofilms. The aggregates tolerate desiccation better than planktonic cells do, adhere and displace even in the absence of the lipopolysaccharide-O antigen, flagella, the transcriptional regulator VjbR, or the enzymes that synthesize, transport, and modify cyclic β ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
1. Targonski P.V, Jacobson R.M, Poland G.A. Immunosenescence: role and measurement in influenza vaccine response among the elderly. Vaccine. 2007;25:3066-3069 2. Noreddin A.M, Haynes V. Use of pharmacodynamic principles to optimise dosage regimens for antibacterial agents in the elderly. Drugs Aging. 2007;24:275-292 3. Liang S.Y, Mackowiak P.A. Infections in the elderly. Clin Geriatr Med. 2007;23:441-456 4. Vasto S, Candore G, Balistreri C.R, Caruso M, Colonna-Romano G. et al. Inflammatory networks in ageing, age-related diseases and longevity. Mech Ageing Dev. 2007;128:83-91 5. High K.P, Prasad R, Marion C.R, Schurig G.G, Boyle S.M. et al. Outcome and immune responses after Brucella abortus infection in young adult and aged mice. Biogerontology. 2007;8:583-593 6. Htwe T.H, Mushtaq A, Robinson S.B, Rosher R.B, Khardori N. Infection in the elderly. Infect Dis Clin North Am. 2007;21:711-743 7. Hakim F.T, Gress R.E. Immunosenescence: deficits in adaptive immunity in the elderly. Tissue Antigens. ...
Lineage: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Brucellaceae; Brucella; Brucella abortus; Brucella abortus bv. ...
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The innate immune system detects the presence of microbes in tissue by pattern recognition of conserved microbial structures, known as pathogen-associated molec...
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Brucella BvrR protein: BvrR/BvrS represent the two-component system involved in the regulation of Brucella virulence; amino acid sequence in first source
Shop a large selection of Proteins A-Z products and learn more about enQuireBio™ Recombinant B. abortus vjbR Protein 100μg enQuireBio™ Recombinant
Very little is known about the role of DNA repair networks in Brucella abortus and its role in pathogenesis. We investigated the roles of RecA protein DNA repair and SOS regulation in B. abortus. While recA mutants in most bacterial species are hyper ...
Luo et al., 2006a: Luo DY, Li P, Xing L, Zhao GY, Shi W, Zhang SL, Wang XL. DNA vaccine encoding L7/L12-P39 of Brucella abortus induces protective immunity in BALB/c mice. Chinese medical journal. 2006 Feb 20; 119(4); 331-4. [PubMed: 16537031 ...
Size, charge and structural heterogeneity of Brucella abortus lipopolysaccharides demonstrated by two-dimensional gel electrophoresis (pages 283-288). Dr. Blair A. Sowa, Richard P. Crawforda, Fred C. Heck, John D. Williams, Albert M. Wu, Katherine A. Kelly and L. Garry Adams. Version of Record online: 16 APR 2008 , DOI: 10.1002/elps.1150070608. ...
Die Abortus Bang Ringprobe (ABR Test) ist eine serologische Untersuchung, die zum Nachweis von Brucellen in Milch genutzt wird. Hierbei werden die Antikörper (IgM, IgG, IgA) mit einem Hämatoxylin gekoppelten Antigen sichtbar demacht. Es handelt…
Bacillus anthracis is a facultative intracellular bacterial pathogen that can cause cutaneous, gastrointestinal or respiratory disease in many vertebrates, including humans. Commercially available anthrax vaccines for immunization of humans are of limited duration and do not protect against the respiratory form of the disease. Brucella abortus is a facultative intracellular bacterium that causes chronic infection in animals and humans. As with other intracellular pathogens, cell mediated immune responses (CMI) are crucial in affording protection against brucellosis. B. abortus strain RB51 has been shown to be useful in eliciting protective cell mediated immunity and humoral responses against Brucella in cattle and other animal species. Since the protective antigen (PA) of B. anthracis is known to induce protective antibodies, it was decided that the objective of this research was to test whether the gene encoding PA could be expressed in Brucella producing a bivalent vaccine to protect against ...
Fluorescence Polarization Assay for the Diagnosis of Anti-Brucella abortus Antibodies in Cattle Serum: Adaptation for its Use in Microplates and Comparison with Conventio..
Accepted name: D-erythritol 1-phosphate dehydrogenase. Reaction: D-erythritol 1-phosphate + NADP+ = D-erythrulose 1-phosphate + NADPH + H+. Other name(s): eryB (gene name). Systematic name: D-erythritol-1-phosphate 2-oxidoreductase. Comments: The enzyme, characterized from the pathogenic bacterium Brucella abortus, which causes brucellosis in livestock, participates in erythritol catabolism.. Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: References:. 1. Sperry, J.F. and Robertson, D.C. Erythritol catabolism by Brucella abortus. J. Bacteriol. 121 (1975) 619-630. [PMID: 163226]. 2. Sangari, F.J., Aguero, J. and Garcia-Lobo, J.M. The genes for erythritol catabolism are organized as an inducible operon in Brucella abortus. Microbiology 146 (2000) 487-495. [PMID: 10708387]. 3. Barbier, T., Collard, F., Zuniga-Ripa, A., Moriyon, I., Godard, T., Becker, J., Wittmann, C., Van Schaftingen, E. and Letesson, J.J. Erythritol feeds the pentose phosphate pathway via three new ...
DO NOT REPLY TO THIS MESSAGE DIRECTLY PLEASE VISIT http://www.medcor.mcgill.ca/EXPMED/DOCS/jobs.html New on ExpMed Job Listings (Post Doc: fluorescence polarization assays (FPA) for the diagnosis of Infectious Disease Min Lin, Ph.D. Animal Diseases Research Institute Immunology Nepean, Ontario, Canada Project description: A post-doctoral position is available immediately to carry out research that leads to development of homogeneous fluorescence polarization assays (FPA) for the diagnosis of infectious diseases. The studies may include identification and characterization of the immunodominant protein/carbohydrate antigens from pathogenic bacteria, molecular cloning, protein expression/purification, epitope mapping, chemical modification of proteins, structure/function analysis of proteins, and assessment of the immune response to antigens. Recent publications: (1)Lin, M., and K. Nielsen. 1997. Binding of the Brucella abortus lipopolysaccharide O-chain fragment to a monoclonal antibody. J. Biol. ...
Looking for online definition of abortus in the Medical Dictionary? abortus explanation free. What is abortus? Meaning of abortus medical term. What does abortus mean?
The results of our study are in agreement with the reports of other workers1,3,15,17, who also demonstrated the presence of B. abortus agglutinins in dog sera using RBT. The higher prevalence of 5.46 % recorded by RBT compared to 0.27 % (only 1 animal) by RSA could be attributed to the practice of feeding dogs with foetuses from slaughtered cows or meat at abattoirs as the prevalence of brucellosis is 6 % in cattle in Nigeria5.. Although not conclusive, our data suggest that young adult dogs (,1 year ) are most affected (Table 1). This is corroborated by a report of higher rates in this age group1and a study that suggested that Brucella infection in dogs is age-dependent15. In addition, we recorded higher prevalences in females (Table 2) but in another study a slightly higher rate in males (29.6 %) than in females (26.7 %) was recorded1. A contributing factor to higher rates in females could be that a single male dog, if infected and used to mate with several females, can transmit the infection ...
Brucellosis is a disease that can be prevented through vaccination. Yet, the effectiveness of the vaccination to fight this disease is considered weak. Fortunately, attempts to modify brucellosis vaccine is still keep going. Some brucellosis vaccines have been found and developed in the past time such as the vaccine B.abortus strain 19-BA and 104M which was made from weakened microbes which had been widely used in Uni Soviet and China. The other brucellosis vaccine that were used in the past were the phenolinsoluble peptidoglycan vaccine which was made in France and polysaccharide-protein vaccine which was used in Russia. This research attempted to see the determinant of antigenic Outer Membrane Protein (OM) 36 kDa Brucella abortus local isolation which has immunogenic character to be developed as an advanced brucellosis vaccine. The method used in this research was the Omp2 gene of Brucella abortus of local isolate employed the PCR technique. The result of the PCR was then sequenced to analyze ...
Zoonotic pathogens can harm human health and wellbeing directly or by impacting livestock. Pathogens that spillover from wildlife can also impair conservation efforts if humans perceive wildlife as pests. Brucellosis, caused by the bacterium Brucella abortus, circulates in elk and bison herds of the Greater Yellowstone Ecosystem and poses a risk to cattle and humans. Our goal was to understand the relative effects of climatic drivers, host demography, and management control programs on disease dynamics. Using | 20 years of serologic, demographic, and environmental data on brucellosis in elk, we built stochastic compartmental models to assess the influences of climate forcing, herd immunity, population turnover, and management interventions on pathogen transmission. Data were collected at feedgrounds visited in winter by free‐ranging elk in Wyoming, USA. Snowpack, hypothesized as a driver of elk aggregation and thus brucellosis transmission, was strongly correlated across feedgrounds. We expected this
The enzyme, characterized from the pathogenic bacterium Brucella abortus, which causes brucellosis in livestock, participates in erythritol catabolism.
Pathway modules Carbohydrate metabolism Central carbohydrate metabolism M00002 Glycolysis, core module involving three-carbon compounds [PATH:bmf00010 bmf01200 bmf01230 bmf01100 ...
The patterns of susceptibility to hydrophobic and hydrophilic drugs and the uptake of the fluorescent probe N-phenyl-naphthylamine in Brucella spp., Haemophilus influenzae, Escherichia coli, and deep rough Salmonella minnesota mutants were compared. The results show that the outer membranes of smooth and naturally rough Brucella spp. do not represent barriers to hydrophobic permeants and that this absence of a barrier relates at least in part to the properties of Brucella lipopolysaccharide. ...
An anti-Brucella vaccine candidate which comprises of purified Brucella lipopolysaccharide and cocktail of four Salmonella Typhimurium (ST)-Brucella vectors was reported earlier. Each vector constitutively express highly conserved Brucella antigens (rB), viz. lumazine synthase (BLS), proline racemase subunit A (PrpA), outer membrane protein-19 (Omp19), and Cu-Zn superoxide dismutase (SOD). The present study determined relative level of protection conferred by each single strain. Upon virulent challenge, the challenge strain was recovered most abundantly in non-immunized control mice and the ST-Omp19, ST-BLS, LPS, and ST-SOD immunized mice groups showed much less burden. Indirect ELISA based assay also confirmed the induction of antigen specific IgG for each antigen delivered. In route-wise comparison of the combined vaccine candidate, intraperitoneal (IP), intramuscular (IM), and subcutaneous (SC) immunizations revealed an indication of highly efficient routes for the protection. Splenocytes of ...
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While macrophages are normally considered to be a primary cell for mediating innate immune responses by phagocytosing and destroying microbes that infect mammals, some microbial organisms including bacteria and protozoa have specifically adapted themselves to survive and replicate in host cells thereby hiding from protective immune responses. We study Brucella abortus, a bacterium that causes disease in a wide variety of agricultural animals and in people, to evaluate how some animals but not others successfully prevent infections by intracellular pathogens. We are particularly interested in the role of host cytokines such as interleukins and gamma-interferon produced by T lymphocytes in controlling the infection through their ability to activate macrophages for antimicrobial activities. In addition we are interested in identifying genes of the bacteria that facilitate their survival in host macrophages thus allowing them to establish chronic infections. Our second major area of research ...
As debate on the merits of mandatory pasteurization of milk intended for cheesemaking continues, global demand for raw-milk cheese is increasing.. While the Food and Drug Administration is conducting a scientific evaluation on the microbiological safety of cheeses made from raw milk, such as Parmigiano Reggiano, Gruyere, Gouda, and Cheddar, it may be useful to review some of the risk assessments and policy discussions that are occurring around the globe.. United States regulations governing the use of raw milk for cheese-making, promulgated in 1949, require cheesemakers to either pasteurize raw milk or hold cheese at no lower than 35°F for a minimum of 60 days. Mycobacterium tuberculosis and Brucella abortus were the pathogens of concern at that time, and aging allowed pathogen inactivation.. As a result of improvements in animal health and disease management, these are no longer primary pathogens of concern. However, Escherichia coli O157:H7, Salmonella typhimurium DT 104, and Listeria ...
Brucella Species answers are found in the Johns Hopkins ABX Guide powered by Unbound Medicine. Available for iPhone, iPad, Android, and Web.
Abortus technieken en methoden Noodanticonceptie: Hormonale methoden door inname van hoge dosissen oestrogenen: Klassieke noodpil (of Yuzpe-methode) of morning-afterpil (MAP), Levonorgestrel (LNG) (progestogeen-enkelpil, noodpil, morning-afterpil), Mifepristone of abortuspil (RU486) - Mechanische Methode: Abortus tijdens het 1e trimester van de zwangerschap: Abortuspil, Vacu maspiratie, Het embryo wordt opgezogen en vervolgens in delen opgevangen, Dilatatie en Curettage. Abortus tijdens het 2e trimester van de zwangerschap: Prostaglandinen, (Te) vroege keizersnede of ...
The primers for the genes of the identified proteins were designed and amplificated by PCR in four Brucella strains. The optimized PCR conditions and the length of PCR products were determined. The results of the PCR amplification in four different Brucella strains were shown. +, Corresponding sequence have been able to amplify; -, Corresponding sequence not been able to amplify ...
When and how often laboratory tests are done may depend on many factors. The timing of laboratory tests may rely on the results or completion of other tests, procedures, or treatments. Lab tests may be performed immediately in an emergency, or tests may be delayed as a condition is treated or monitored. A test may be suggested or become necessary when certain signs or symptoms appear. Due to changes in the way your body naturally functions through the course of a day, lab tests may need to be performed at a certain time of day. If you have prepared for a test by changing your food or fluid intake, lab tests may be timed in accordance with those changes. Timing of tests may be based on increased and decreased levels of medications, drugs or other substances in the body. The age or gender of the person being tested may affect when and how often a lab test is required. Chronic or progressive conditions may need ongoing monitoring through the use of lab tests. Conditions that worsen and improve may ...
Eksploration: uterus st rrelse, lejring og adnexae vurderes. En evt. 2 klot nger i forreste portiol be. Dilatation til hegar -1 over sugets st rrelse. Sugets st rrelse svarer til gestationsalderen. Suget indf res til fundus uden vacuum. Vacuum tilsluttes og kaviteten t mmes. Der g s efter med stump curette, specielt i hj rnerne. Minimal bl dning og en velkontraheret uterus tyder p , at kaviteten er tom. Det kontrolleres ved abdominal ultralydsscanning, at kaviteten er tom, ligesom ultralydsscanning kan anvendes ved indf ring af suget, hvis der er i tvivl om uteri lejring. Afvaskning med klorhexidin 0,05% mindsker ikke infektionsraten ...
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Kang, Yoon-Suk, Daniel A. Brown, and James E. Kirby. Brucella neotomae Recapitulates Attributes of Zoonotic Human Disease in a Murine Infection Model. Infection and Immunity 87.1 (2019): e00255-18. Web. 08 April. 2020. ...
... detection of antibody to Brucella abortus". Methods. 22 (1): 71-6. doi:10.1006/meth.2000.1038. PMID 11020320. Jameson D (2003 ...
Pei J, Ficht TA (January 2004). "Brucella abortus rough mutants are cytopathic for macrophages in culture". Infection and ... Ugalde JE, Czibener C, Feldman MF, Ugalde RA (October 2000). "Identification and characterization of the Brucella abortus ... Ugalde JE, Comerci DJ, Leguizamón MS, Ugalde RA (November 2003). "Evaluation of Brucella abortus phosphoglucomutase (pgm) ... "Establishment of systemic Brucella melitensis infection through the digestive tract requires urease, the type IV secretion ...
Currently, all cattle of ages 3-8 months must receive the Brucella abortus strain 19 vaccine. Australia is free of cattle ... Brucella species survive well in aerosols and resist drying. Brucella and all other remaining biological weapons in the U.S. ... Four species infect humans: B. abortus, B. canis, B. melitensis, and B. suis. B. abortus is less virulent than B. melitensis ... Brucella species have also been isolated from several marine mammal species (cetaceans and pinnipeds). B. abortus is the ...
"Successful Medical Treatment of Prosthetic Mitral Valve Endocarditis Caused by Brucella abortus". Korean Circulation Journal. ...
Danish veterinarian Bernhard Bang isolates Brucella abortus as the agent of Brucellosis. L. Emmett Holt publishes the standard ...
He discovered Brucella abortus in 1897, which came to be known as Bang's bacillus. Bang's bacillus was the cause of the ... "Bacillus abortus" by the American microbiologist Alice Evans]", Zeitschrift für ärztliche Fortbildung, 82 (8), pp. 381-5, PMID ...
"The Brucella abortus CcrM DNA Methyltransferase Is Essential for Viability, and Its Overexpression Attenuates Intracellular ... the plant symbiont Sinorhizobium meliloti and in the human pathogen Brucella abortus. Also CcrM gene has proven to be essential ...
He was chairman of the committee on Brucella abortus infection and on Johne's disease (Paratuberculosis). He also chaired the ...
A Case of Identity Confirmation of Brucella abortus S99 by Phage Typing and PCR Methods. Cowley LA, Low AS, Pickard D, Boinett ...
"Brucella abortus Transits through the Autophagic Pathway and Replicates in the Endoplasmic Reticulum of Nonprofessional ...
Pouvoir inhibiteur de ce produit vis-à-vis du bacille typhique, du colibacille, de Brucella abortus etc". Comptes Rendus de ... and Brucea abortus. Clitocine has also been shown to promote apoptosis (cell death) in human cervical cancer cells in vitro ( ...
Liposomised recombinant ribosomal L7/L12 protein protects BALB/c mice against Brucella abortus 544 infection. Vaccine. 2007, 4; ... He had successfully expressed L7/L12 ribosomal protein, SOD-IL-18 fusion protein of Brucella spp. and trypanothione reductase ... entrapped DNA vaccine co-expressing Cu-Zn superoxide dismutase and IL-18 confers protection against Brucella abortus". Microbes ... the protective efficacy of liposomes encapsulated recombinant Cu-Zn superoxide dismutase protein against Brucella abortus. ...
Some bacteria like Brucella abortus, Moraxella catarrhalis and Streptococcus pneumoniae have developed resistance towards ...
Antitumor process using a Brucella abortus preparation, retrieved 2016-01-26 Selected Patents Dowling, Patricia W.; Youngner, ... antitumor processes of Brucella, and numerous contributions to cold adapted influenza virus. Youngner met and married his first ...
Brucella abortus *Brucellosis. Bartonellaceae. *Bartonellosis: Bartonella henselae *Cat-scratch disease. *Bartonella quintana * ...
Brucella abortus *Brucellosis. Bartonellaceae. *Bartonellosis: Bartonella henselae *Cat-scratch disease. *Bartonella quintana * ...
Brucella abortus *Brucellosis. Bartonellaceae. *Bartonellosis: Bartonella henselae *Cat-scratch disease. *Bartonella quintana * ...
Brucella abortus *Brucellosis. Bartonellaceae. *Bartonellosis: Bartonella henselae *Cat-scratch disease. *Bartonella quintana * ...
Brucella abortus *Brucellosis. Bartonellaceae. *Bartonellosis: Bartonella henselae *Cat-scratch disease. *Bartonella quintana * ...
Brucella abortus *Brucellosis. Bartonellaceae. *Bartonellosis: Bartonella henselae *Cat-scratch disease. *Bartonella quintana * ...
Brucella abortus *Brucellosis. Bartonellaceae. *Bartonellosis: Bartonella henselae *Cat-scratch disease. *Bartonella quintana * ...
Brucella abortus *Brucellosis. Bartonellaceae. *Bartonellosis: Bartonella henselae *Cat-scratch disease. *Bartonella quintana * ...
Brucella abortus *Brucellosis. Bartonellaceae. *Bartonellosis: Bartonella henselae *Cat-scratch disease. *Bartonella quintana * ...
Brucella abortus *Brucellosis. Bartonellaceae. *Bartonellosis: Bartonella henselae *Cat-scratch disease. *Bartonella quintana * ...
2005). "Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella ... 2002). "The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts". Proc. Natl ... 2005). "The Chlamydophila abortus genome sequence reveals an array of variable proteins that contribute to interspecies ... 2002). "The genome sequence of the facultative intracellular pathogen Brucella melitensis". Proc. Natl. Acad. Sci. U.S.A. 99 (1 ...
Elimination of Brucella abortus from infected domestic (Bos taurus) and wild (Bos gaurus) cattle and buffalo (Syncerus caffer) ...
Studies on the bactericidal action of bovine whole blood and serum towards Brucella abortus and Brucella suis. J. Infect. Dis. ...
It can be caused by infection from Actinomyces bovis or Brucella abortus organisms, but may also occur due to parasite ... Because of modern efforts to reduce the incidence of brucellosis in livestock, horses are less exposed to the Brucella abortus ...
It is normally active against Moraxella catarrhalis, Brucella abortus, Burkholderia cepacia, Citrobacter species, Escherichia ...
... of the deceased fetus causes necrosis of the placenta and placental lesions are not easily distinguished from Brucella abortus ... stomach contents or tissue of abortuses. Samples should be immediately transported to the lab and enrichment needs to be ...
For example, Agrobacterium tumefaciens is a plant pathogen, Brucella abortus is an animal pathogen, and Sinorhizobium meliloti ...
Brucella spp. cattle, goats infected milk or meat Bubonic plague, Pneumonic plague, Septicemic plague, Sylvatic plague Yersinia ... Chlamydophila abortus domestic livestock, particularly sheep close contact with postpartum ewes COVID-19 severe acute ...
"5-Lipoxygenase negatively regulates Th1 response during Brucella abortus infection in mice". Infection and Immunity. 83 (3): ... ALOX5 gene knockout mice demonstrate an enhanced resistance and lessened pathology to Brucella abortus infection[31] and, at ...
Brucella spp.. cattle, goats. infected milk or meat. Bubonic plague, Pneumonic plague, Septicemic plague. Yersinia pestis. ... Chlamydophila abortus. domestic livestock, particularly sheep. close contact with postpartum ewes. Variant Creutzfeldt-Jakob ...
Brucella abortus (Brucellosis). Bartonellaceae. Bartonellosis: Bartonella henselae (പൂച്ചമാന്തി രോഗം) · Bartonella quintana ( ...
A functional water channel protein in the pathogenic bacterium Brucella abortus. Microbiol 146(12):3251-3257. doi: 3251-3257. ... seperti Brucella abortus yang menyebabkan brucellosis pada sapi dan Bacillus anthracis yang menyebabkan antraks.[60] Untuk ...
Brucella abortus *Brucellosis. Bartonellaceae. *Bartonellosis: Bartonella henselae *Cat-scratch disease. *Bartonella quintana * ...
Brucella abortus Brucellosis ing sapi 2. Streptococcus agalactia Mastitis ing sapi (radang payudara) ...
Brucella abortus (Brucellosis). Bartonellaceae. Bartonellosis: Bartonella henselae (Cat scratch disease) · Bartonella quintana ...
... with Brucella abortus Strain RB51 Overexpressing Superoxide Dismutase and Glycosyltransferase Genes Does Not Induce Adequate ... Protection against Experimental Brucella abortus Challenge". Frontiers in Cellular and Infection Microbiology. 6: 10. doi: ...
... abortus A13334, 3,401 ORFs Brucella canis ATCC 23365, 3,408 ORFs Brucella melitensis 16M, 3,279 ORFs Brucella microti ... 3,193 ORFs Brucella pinnipedialis B2/94, 3,505 ORFs Brucella suis 1330, 3,408 ORFs Genome data for these and other Brucella ... Many Brucella sRNAs have been identified. Infection of macrophages by B. abortus is stimulated by blue light in the wild type, ... Brucella species have been found primarily in mammals: Pathogenic Brucella species can cause abortion in female animals by ...
Brucellosis is caused by different biotypes of Brucella abortus and B. melitensis.[52] Other internal parasites include ...
The Brucella abortus (rough LPS Brucella) vaccine, developed for bovine brucellosis and licensed by the USDA Animal Plant ... of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella ... "Brucella abortus cyclic beta-1,2-glucan mutants have reduced virulence in mice and are defective in intracellular replication ... Since brucellosis threatens the food supply and causes undulant fever, Brucella suis and other Brucella species (B. melitensis ...
B. abortus also affects bison. Brucella has twelve different kinds of species, one being Brucella abortus. Some of the other ... The prokaryotic B. abortus is non-spore-forming, non-motile and aerobic. Brucella abortus enters phagocytes that invade human ... "Humans and Brucella Species". "Brucellosis: Brucella Abortus" (PDF). Kaden, R.; Ferrari, S.; Jinnerot, T.; Lindberg, M.; Wahab ... Brucella abortus is a Gram-negative alpha-proteobacterium in the family Brucellaceae and is one of the causative agents of ...
... as well as in a broad spectrum of Brucella species (B. ovis, B. canis, B. abortus and B. microtis, and several viobars of B. ... viciae 3841 strain, and the Agrobacterium species A. vitis,A. tumefaciens, A. radiobacter and A. H13, Brucella species (B. ovis ... B. canis, B. abortus, B. microtis, and several biobars of B. melitensis), Ochrobactrum anthropi,the Mesorhizobium species loti ... Partial synteny of the αr45 genomic regions was observed in the Mesorhizobium and Brucella species where instead of aLysR ...
B. abortus also affects bison. Brucella has twelve different kinds of species, one being Brucella abortus. Some of the other ... The prokaryotic B. abortus is non-spore-forming, non-motile and aerobic. Brucella abortus enters phagocytes that invade human ... "Humans and Brucella Species". "Brucellosis: Brucella Abortus" (PDF). Kaden, R.; Ferrari, S.; Jinnerot, T.; Lindberg, M.; Wahab ... Brucella abortus is a Gram-negative alpha-proteobacterium in the family Brucellaceae and is one of the causative agents of ...
... abortus,biological,advanced biology technology,biology laboratory technology,biology device technology,latest biology ... Multiporator / Electropo... Making electrocompetent cells: ... Electroporation of cells: Grow ...,Brucella, ... 4308 915.508 12/2001 Microorganism Brucella abortus Cell type Bacteria, gram negative Molecules injected Plasmid DNA (pBA.sodkn ...
... On May 26-27, 1997, nine persons (a farmer, four veterinary ... Experimental infection of pregnant cattle with the vaccine candidate Brucella abortus strain RB51: pathologic, bacteriologic, ... and a necropsy on a stillborn calf that died because of Brucella abortus infection. The infection was confirmed by isolation of ... vaccinated with Brucella abortus strain RB51. J Vet Diagn Invest 1997;9:363-7. ...
An Unusual Case of Brucella abortus Prosthetic Joint Infection.. Walsh J1, Gilleece A2, Fenelon L1, Cogley D3, Schaffer K1. ... Brucellosis is a systemic infection caused by brucella species. Prosthetic joint infection due to brucella species is rare. We ...
Brucella abortus strain BDW chromosome 2, complete sequence Brucella abortus strain BDW chromosome 2, complete sequence. gi, ...
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Methane metabolism - Brucella abortus 2308 [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show description , ...
M00130 Inositol phosphate metabolism, PI=> PIP2 => Ins(1,4,5)P3 => Ins(1,3,4,5)P4 ...
Distribution of Radioiodinated, Acetone-killed Brucella abortus in the Tissues of Normal and Immune Mice. *D. SULITZEANU1. ... SULITZEANU, D. Distribution of Radioiodinated, Acetone-killed Brucella abortus in the Tissues of Normal and Immune Mice. Nature ... Induced Resistance of Mice to Infection with Brucella abortus 2308 through Vaccination with BCG *A. BEKIERKUNST ...
Signes cliniques photos: Brucella abortus. Information sur les maladies animales Description:. Bovin, placenta. De nombreux ...
This work demonstrates that Brucella lipopolysaccharide (LPS) preparations are a family of related molecules which display ... Heterogeneity of Brucella Abortus Lipopolysaccharides Res Microbiol. 1995 Sep;146(7):569-78. doi: 10.1016/0923-2508(96)80563-8 ... Smooth-type LPS (S-LPS) from B. abortus demonstrated three broad high-molecular-weight bands corresponding to S-LPS, and a low- ... This work demonstrates that Brucella lipopolysaccharide (LPS) preparations are a family of related molecules which display ...
This assay utilizes antigen from Brucella abortus strain W99. ... for the measurement of human IgA antibodies to Brucella abortus ... The Brucella abortus IgA ELISA Kit is intended ... Brucella abortus IgA ELISA Kit (CE). Packaging size: 96 Wells ... The Brucella abortus ELISA Kit is intended for the measurement of human IgG, IgA, or IgM antibodies to Brucella abortus. This ... The Brucella abortus IgA ELISA Kit is intended for the measurement of human IgA antibodies to Brucella abortus. This assay ...
... MMWR 47(09);172-175 Publication date: 03/13/1998. Table of ... Experimental infection of pregnant cattle with the vaccine candidate Brucella abortus strain RB51: pathologic, bacteriologic, ... and a necropsy on a stillborn calf that died because of Brucella abortus infection. The infection was confirmed by isolation of ... a stable rough strain of Brucella abortus. Vet Microbiol 1991;28:171-88. * Cheville NF, Jensen AE, Halling SM, et al. Bacterial ...
cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › BrucellaBrucella abortus ... sp,Q2YK69,NANM_BRUA2 N-acetylneuraminate epimerase OS=Brucella abortus (strain 2308) OX=359391 GN=nanM PE=3 SV=1 ... Brucella abortus (strain 2308). ,p>This subsection of the ,a href="http://www.uniprot.org/help/names_and_taxonomy_section"> ...
Brucella abortus strain 2308. Brucella abortus (strain 2308): entries and gene names ... cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › BrucellaBrucella abortus ... sp,P0C0Z6,RS15_BRUA2 30S ribosomal protein S15 OS=Brucella abortus (strain 2308) OX=359391 GN=rpsO PE=3 SV=1 ... Brucella abortus (strain 2308). ,p>This subsection of the ,a href="http://www.uniprot.org/help/names_and_taxonomy_section"> ...
0612 are highly over-produced in a Brucella abortus hfq mutant compared with the parental strain, … ... Identification of two small regulatory RNAs linked to virulence in Brucella abortus 2308 Mol Microbiol. 2012 Jul;85(2):345-60. ... Orthologous sRNAs (likewise designated AbcR1 and AbcR2) have been identified in B. abortus 2308. In Brucella, abcR1 and abcR2 ... 0612 are highly over-produced in a Brucella abortus hfq mutant compared with the parental strain, and recently, expression of ...
... foi empregada na detecção de Brucella spp. em 300 amostras de produtos... ... utilizando-se primers desenhados a partir do gene que codifica uma proteína imunogênica de 31 KDa de Brucella abortus, ... Todas as amostras positivas na PCR para Brucella spp. foram confirmadas como sendo da espécie Brucella abortus pela técnica de ... All positive samples for Brucella spp. were confirmed as Brucella abortus by PCR using species-specific primers. Hemi-nested ...
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Blocking Serum Lysis of Brucella Abortus by Hyperimmune Rabbit Immunoglobulin A. Wendell H. Hall, Robert E. Manion, Horace H. ... Blocking Serum Lysis of Brucella Abortus by Hyperimmune Rabbit Immunoglobulin A. Wendell H. Hall, Robert E. Manion, Horace H. ... Blocking Serum Lysis of Brucella Abortus by Hyperimmune Rabbit Immunoglobulin A. Wendell H. Hall, Robert E. Manion and Horace H ... Blocking Serum Lysis of Brucella Abortus by Hyperimmune Rabbit Immunoglobulin A Message Subject (Your Name) has forwarded a ...
The two-component system BvrR/BvrS essential for Brucella abortus virulence regulates the expression of outer membrane proteins ... The two-component system BvrR/BvrS essential for Brucella abortus virulence regulates the expression of outer membrane proteins ... The two-component system BvrR/BvrS essential for Brucella abortus virulence regulates the expression of outer membrane proteins ... The two-component system BvrR/BvrS essential for Brucella abortus virulence regulates the expression of outer membrane proteins ...
Pathway modules Carbohydrate metabolism Central carbohydrate metabolism M00002 Glycolysis, core module involving three-carbon compounds [PATH:bmf00010 bmf01200 bmf01230 bmf01100 ...
... sequencing to rapidly and comprehensively determine the complete genome sequence of the attenuated Brucella abortus vaccine ... abortus, 9-941 and 2308. The two S19 chromosomes are 2,122,487 and 1,161,449 bp in length. A total of 3062 genes were ... The Brucella abortus strain S19, a spontaneously attenuated strain, has been used as a vaccine strain in vaccination of cattle ...
Brucella abortus,/i,. Both lived on a dairy cattle farm with a history of regular cases of abortion and mastitis. ... "Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR," ... Trueperella pyogenes and Brucella abortus Coinfection in a Dog and a Cat on a Dairy Farm in Egypt with Recurrent Cases of ... Trueperella pyogenes and Brucella abortus Coinfection in a Dog and a Cat on a Dairy Farm in Egypt with Recurrent Cases of ...
5-Tetrahydropyridine-2-carboxylate N-Succinyltransferase from Brucella melitensis biovar abortus 2308 ... Crystal structure of 2,3,4,5-Tetrahydropyridine-2-carboxylate N-Succinyltransferase from Brucella melitensis biovar abortus ...
Brucella Abortus Lipopolysaccharide Is Mitogenic for Spleen Cells of Endotoxin-Resistant C3H/HeJ Mice. Edgardo Moreno and David ... Brucella Abortus Lipopolysaccharide Is Mitogenic for Spleen Cells of Endotoxin-Resistant C3H/HeJ Mice ... Preparations of lipopolysaccharide (LPS) from rough and smooth strains of Brucella abortus were mitogenic for spleen cells of ... Brucella Abortus Lipopolysaccharide Is Mitogenic for Spleen Cells of Endotoxin-Resistant C3H/HeJ Mice ...
B. abortus infection induces apoptosis and inhibits osteoblast function. DHEA treatment reversed the effect of B. abortus ... B. abortus infection induces apoptosis and inhibits osteoblast function. DHEA treatment reversed the effect of B. abortus ... abortus on osteoblast differentiation and function. In contrast, cortisol increased the effect of B. abortus infection. ... abortus on osteoblast differentiation and function. In contrast, cortisol increased the effect of B. abortus infection. ...
By confocal microscopy, we demonstrated that TLR7 is colocalized with B. abortus in LAMP-1+ Brucella-containing vacuoles. ... By confocal microscopy, we demonstrated that TLR7 is colocalized with B. abortus in LAMP-1+ Brucella-containing vacuoles. ... Our results suggest that TLR3 and TLR7 are not required to control Brucella infection in vivo but they play an important role ... Our results suggest that TLR3 and TLR7 are not required to control Brucella infection in vivo but they play an important role ...
The Brucella abortus cyclic beta-1,2-glucan virulence factor is substituted with O-ester-linked succinyl residues. J Bacteriol ... G1-arrested newborn cells are the predominant infectious form of the pathogen Brucella abortus. Nat Commun 5:4366. doi:10.1038/ ... Brucella abortus Cgs is a 320-kDa (2,867-amino-acid-residue) polytopic integral inner membrane protein with six transmembrane ... Brucella abortus cyclic beta-1,2-glucan mutants have reduced virulence in mice and are defective in intracellular replication ...
Deletion of the BCSP31 gene of Brucella abortus by replacement.. S M Halling, P G Detilleux, F M Tatum, B A Judge, J E Mayfield ... Deletion of the BCSP31 gene of Brucella abortus by replacement.. S M Halling, P G Detilleux, F M Tatum, B A Judge, J E Mayfield ... Deletion of the BCSP31 gene of Brucella abortus by replacement.. S M Halling, P G Detilleux, F M Tatum, B A Judge, J E Mayfield ... Deletion of the BCSP31 gene of Brucella abortus by replacement. Message Subject (Your Name) has forwarded a page to you from ...
Deletion of the BCSP31 gene of Brucella abortus by replacement.. S M Halling, P G Detilleux, F M Tatum, B A Judge, J E Mayfield ... Deletion of the BCSP31 gene of Brucella abortus by replacement.. S M Halling, P G Detilleux, F M Tatum, B A Judge, J E Mayfield ... Deletion of the BCSP31 gene of Brucella abortus by replacement.. S M Halling, P G Detilleux, F M Tatum, B A Judge, J E Mayfield ... abortus strain 19 (S19), into a rough variant of B. abortus S19, and into B. abortus S2308, and antibiotic-resistant ...
  • Brucella abortus is a Gram-negative alpha-proteobacterium in the family Brucellaceae and is one of the causative agents of brucellosis. (wikipedia.org)
  • Brucellosis: Brucella Abortus" (PDF). (wikipedia.org)
  • None of the exposed persons reported having previously had brucellosis or being unintentionally inoculated with Brucella vaccine. (cdc.gov)
  • Editorial Note: Brucellosis, also known as 'undulant fever' or 'Bangs disease,' is a systemic infection caused by Brucella sp. (cdc.gov)
  • Brucellosis is a systemic infection caused by brucella species. (nih.gov)
  • Uterine discharges were collected from the bitch and the cat under sterile conditions after positive Brucella serology using Rose Bengal Test and Complement Fixation Test and sent to the OIE reference laboratory of brucellosis at the Friedrich-Loeffler-Institut for Brucella identification and biotyping. (hindawi.com)
  • Osteoarticular brucellosis is the most common presentation of the active disease in humans, and we have previously demonstrated that B. abortus infection inhibits osteoblast function. (frontiersin.org)
  • We conclude that DHEA intervention improves osteoblast function during B. abortus infection making it a potential candidate to ameliorate the osteoarticular symptoms of brucellosis. (frontiersin.org)
  • Among many stratagems employed by the bacterium to harm bone, Brucella can infect and survive within human and murine osteoblasts, and this infection triggers the secretion of receptor activator of nuclear factor-κB ligand (RANKL), proinflammatory cytokines, and chemokines that could be implicated in the presentation of osteoarticular brucellosis. (frontiersin.org)
  • Brucella abortus is a Gram-negative, facultative intracellular bacterium that causes brucellosis, a worldwide zoonotic disease leading to undulant fever in humans and abortion in cattle. (frontiersin.org)
  • B. abortus S19 is an attenuated strain used as a vaccine for prevention of bovine brucellosis in the United States, and B. abortus S2308 is a commonly used challenge strain. (asm.org)
  • The IDEXX Brucellosis Serum X2 Ab Test is an enzyme immunoassay for the detection of antibodies against Brucella abortus in individual serum and plasma, and pools of up to 10 individual serum or plasma samples of ruminants. (idexx.com.au)
  • Brucellosis in cattle is a disease caused by Brucella abortus ( B. abortus ), a facultative, intracellular bacterium. (idexx.com.au)
  • Thirty-five serum samples and six hygroma fluid samples were collected from sexually mature cattle in one herd with clinical signs of brucellosis (abortion and hygromas) in the Western Region of the Gambia in order to isolate and characterise Brucella species. (ebscohost.com)
  • Sustained and differential antibody responses to virulence proteins of Brucella melitensis during acute and chronic infections in human brucellosis. (ebscohost.com)
  • Brucellosis is an important zoonotic disease caused primarily by the bacterial pathogens Brucella melitensis and B. abortus. (ebscohost.com)
  • traditionally bovines are host to B. abortus and B. canis is associated with canine brucellosis. (scielo.org.za)
  • Therefore, the objective of this study was to evaluate Brucella abortus S19 vaccine in cattle naturally infected with brucellosis under tropical conditions. (scialert.net)
  • Brucella abortus , a smooth strain of the genus Brucella , is the causative agent of bovine brucellosis. (asm.org)
  • Epidemiological study of Brucellosis in cattle, immunized with Brucella abortus RB51 vaccine in endemic zones. (semanticscholar.org)
  • Since the protective antigen (PA) of B. anthracis is known to induce protective antibodies, it was decided that the objective of this research was to test whether the gene encoding PA could be expressed in Brucella producing a bivalent vaccine to protect against both brucellosis and anthrax. (vt.edu)
  • Brucellosis, caused by Brucella spp. (ac.ir)
  • It has been used for more than six decades as vaccine that protects cattle against brucellosis, an infectious disease caused by other strains of B. abortus that leads to reproductive failure in livestock. (vt.edu)
  • Using classical biotyping and the Brucella AMOS PCR assay, all isolates were identified as Brucella melitensis biovar 3 (n = 58), B. melitensis biovar 1 (n = 1), Brucella abortus (n = 5), or Brucella suis biovar 3 (n = 2), and B. melitensis biovar 3 was found to be mainly responsible for sheep brucellosis in northwest China. (readbyqxmd.com)
  • B. abortus is the principal cause of brucellosis in cattle and the bacteria can be shed from infected animal during the time of calving or abortion. (elisakits.co.uk)
  • Brucella abortus vaccines play a central role in bovine brucellosis control/eradication programs and have been successfully used worldwide for decades. (beds.ac.uk)
  • In this paper, we present a review of the main aspects of the vaccines that have been used in the brucellosis control over the years and the current research advances in the development of new B. abortus vaccines. (beds.ac.uk)
  • Brucellosis seroprevalence in Bali cattle with reproductive failure in South Sulawesi and Brucella abortus biovar 1 genotypes in the Eastern Indonesian archipelago. (nih.gov)
  • Brucellosis is one of the common zoonoses caused by Brucella abortus (B. abortus). (bvsalud.org)
  • Brucella abortus is the etiological agent of a worldwide zoonosis called brucellosis. (biomedcentral.com)
  • Bovine brucellosis caused by Brucella abortus has far reaching animal health and economic impacts at both the local and national levels. (cdc.gov)
  • Brucellosis, caused by Brucella abortus, is a major disease of cattle and humans worldwide distributed. (cdc.gov)
  • We report a case of Brucella abortus-induced brucellosis that displayed high hepatic transaminases similar to that in acute hepatitis, fourth month relapsed following doxycycline-streptomycin treatment, and developed doxycycline-related hepatotoxicity in second treatment. (medeniyetmedicaljournal.org)
  • Brucellosis is a disease with implications in the public health due both to its zoonotic character as its easy transmission trough of dairy products contaminated with the microorganism Brucella sp. (uptc.edu.co)
  • These findings suggest a mechanism for neutropenia in chronic brucellosis and reveal a novel Brucella-host cross-talk through which B. abortus is able to hinder the innate function of PMN. (una.ac.cr)
  • PHE1 is a htrA cycL double gene deletion mutant of virulent Brucella abortus strain 2308 (S2308) which has previously been evaluated in the murine and caprine models of bovine brucellosis. (lsu.edu)
  • Brucella abort us is a bacterium of brucellosis causing abortion in cattle. (mendeley.com)
  • The diagnosis of bovine brucellosis mainly relies on serologic tests using smooth lipopolysaccharide (S-LPS) from B. abortus. (mendeley.com)
  • These results suggested that B. abortus EryC would be a potential reagent for diagnosis for bovine brucellosis as a single protein antigen. (mendeley.com)
  • Orthologous sRNAs (likewise designated AbcR1 and AbcR2) have been identified in B. abortus 2308. (nih.gov)
  • In an Escherichia coli two-plasmid reporter system, overexpression of either AbcR1 or AbcR2 was sufficient for regulation of target mRNAs, indicating that the AbcR sRNAs from B. abortus 2308 perform redundant regulatory functions. (nih.gov)
  • The effects of B. abortus strain 2308 on bovine PMNs were examined to determine which PMN functions were impaired. (iastate.edu)
  • To determine the placental tropism and abortigenicity of the vaccine candidate Brucella abortus strain RB51 (SRB51), a rough mutant of the virulent strain 2308, ten Polled Hereford heifers were inoculated intravenously in the 6th month of gestation. (semanticscholar.org)
  • We used Brucella reference strains ( B. melitensis 16M and B. abortus 2308) as positive controls and double-distilled water as a negative control. (cdc.gov)
  • small Gram-negative coccobacilli that can infect cattle (infection with B. abortus), goats and sheep (B. melitensis), pigs (B. suis), and dogs (B. canis). (cdc.gov)
  • MLVA Genotyping of Brucella melitensis and Brucella abortus Isolates from Different Animal Species and Humans and Identification of Brucella suis Vaccine Strain S2 from Cattle in China. (ebscohost.com)
  • Clinical and Diagnostic Aspects of Relapsing Meningoencephalitis due to Brucella suis. (ebscohost.com)
  • these are B. abortus , B. melitensis , and B. suis which preferentially infect cattle, small ruminants and swine respectively. (biomedcentral.com)
  • Accurate typing procedures are critical for eradication and control of disease-causing organisms, but DNA-based techniques are challenging because the various Brucella species and strains have a high degree of genetic homology, as demonstrated by whole-genome comparative analyses of the 3 Brucella genomes sequenced, Brucella melitensis ( 4 ), Brucella suis ( 5 ), and Brucella abortus ( 6 ). (aaccjnls.org)
  • B. ovis was distinguished by the absence of the 1682-bp fragment, B. abortus by the absence of the 1071-bp fragment, B. suis by the presence of an additional 272-bp fragment (also present in B. canis and B. neotomae ), B. canis by the absence of the 794-bp fragment, and B. neotomae by the absence of the 152-bp fragment. (aaccjnls.org)
  • Halling SM, Peterson-Burch BD, Bricker BJ, Zuerner RL, Qing Z, Li LL, Kapur V, Alt DP and Olsen SC: Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis . (spandidos-publications.com)
  • Brucella are non-motile, rod-shaped, gram-negative bacteria which can infect a number of different livestock such as cattle, elk and bison ( B. abortus ), dogs ( B. canis ), sheep and goats ( B. melitensis ) and pigs and caribou ( B. suis ). (elisakits.co.uk)
  • abstract = "A sensitive radioimmunoassay for the detection of Brucella abortus antibody is described. (elsevier.com)
  • Both animals lived on a dairy farm with recurrent cases of abortion and mastitis due to Brucella (B.) abortus biovar (bv) 1 infections [ 7 ]. (hindawi.com)
  • Real time PCR (IS 711 ), Bruce-ladder and classical biotyping classified the isolates as B. abortus biovar 3. (biomedcentral.com)
  • These genotypes were not related to B. abortus biovar 3 reference strain Tulya originally isolated from a human patient in Uganda in 1958, unlike the genotypes isolated and characterized recently in Kenya. (biomedcentral.com)
  • This is the first isolation and characterization of B. abortus biovar 3 from a dairy cow with abortion in Tanzania. (biomedcentral.com)
  • The origin of the Tanzanian genotypes remain elusive, although they seem to be related to genotypes found in Europe, Turkey and China but not related to B. abortus biovar 3 reference strain or genotypes from Kenya. (biomedcentral.com)
  • B. abortus biovar 1 was freshly isolated from the hygromas of two cows and together with thirty banked isolates collected since 1990 from different parts of Sulawesi and Timor eight related genotypes could be distinguished with one genotype being identical to that of an isolate (BfR91) from Switzerland. (nih.gov)
  • The Indonesian genotypes formed together with BfR91 and one African and one North American isolate a distinct branch on the B. abortus biovar 1 dendogram. (nih.gov)
  • Dendogram based on MLVA-16 genotyping showing the relationship of 49 B. abortus biovar 1 strains originating from various continents including Europe, South, Central and North America, Africa and Asia whereby the Indonesian genotype BInd33 is identical to genotype BfR91 from Switzerland. (nih.gov)
  • Forty-two MLVA-16 genotypes have been reported for B. abortus biovar 1 isolates from the old and new world and Africa combined [20,21], which together with the eight genotypes determined for the Indonesia isolates makes 49 distinct B. abortus biovar 1 genotypes. (nih.gov)
  • The different MLVA-16 panel 1 and 2A loci for this global collection of B. abortus biovar 1 genotypes showed limited variation with 1, 2 or 3 alleles only and a modest variation was observed for four of the panel 2B locus with a maximum D value of 0.729 and with 6 alleles observed for MLVA-16 locus bruce16 (Table 6). (nih.gov)
  • The maximum distance between the genotypes of this collection of 44 B. abortus biovar 1 genotypes was ten. (nih.gov)
  • In the dendogram constructed for this global collection of B. abortus biovar 1 genotypes from Europe, Africa and the Americans, the Indonesian isolates locate on a branch together with three genotypes originally detected in Europe (BfR91), Africa (BfR96) and North America (BCCNV5) (Figure 2). (nih.gov)
  • We isolated Brucella melitensis biovar 3 from the spleen of an Asian badger ( Meles leucurus ) in Nilka County, northwestern China. (cdc.gov)
  • The National Animal Disease Center, U.S. Department of Agriculture (USDA), identified the B. abortus isolate from the calf as the RB51 vaccine strain. (cdc.gov)
  • The vaccine had caused active B. abortus infection because the 14-month-old heifer delivering the calf was not known to be pregnant when she was vaccinated with RB51 at approximately 8 months of age, which was within the specified age range for vaccination. (cdc.gov)
  • Evidence that intrauterine infection was caused by the RB51 vaccine strain, and not by field strains of B. abortus or by S19, included immunohistochemical staining specific for RB51 (negative for S19), RB51-specific titer of greater than 1:10,000 on experimental dot-blot assay measuring antibody to RB51, and RB51-specific DNA sequences identified by polymerase chain reaction (PCR). (cdc.gov)
  • Antigenic, Immunologic and Genetic Characterization of Rough Strains B.abortus RB51, B.melitensis B115 and B.melitensis B18. (ebscohost.com)
  • Safety Of Brucella Abortus Strain Rb51 Vaccine In Non-Target U" by Terry J. Kreeger, Thomas J. DeLiberto et al. (unl.edu)
  • n =24) were given a single oral dose of at least 1.0X10 10 colony-forming units of Brucella abortus strain RB51 vaccine (RB51). (unl.edu)
  • Experimental infection of pregnant cattle with the vaccine candidate Brucella abortus strain RB51: pathologic, bacteriologic, and serologic findings. (semanticscholar.org)
  • Abortion and premature birth in cattle following vaccination with Brucella abortus strain RB51. (semanticscholar.org)
  • B. abortus strain RB51 has been shown to be useful in eliciting protective cell mediated immunity and humoral responses against Brucella in cattle and other animal species. (vt.edu)
  • The pBB/PA plasmid was used to transform B. abortus RB51 and CmR clones screened for the expression of PA by immunoblotting. (vt.edu)
  • The vaccine strain B. abortus RB51 was readily distinguished by a specific additional 2524-bp fragment and by the absence of the 1682-bp fragment, and the vaccine strain B. abortus S19 did not produce the 587-bp fragment common to all other Brucella strains tested. (aaccjnls.org)
  • Strain 19 and RB51 are the approved B. abortus vaccines strains most commonly used to protect cattle against infection and abortion. (beds.ac.uk)
  • Despite enormous advances and the development of B. abortus S19 and RB51 vaccines, the search for improved vaccines has never ends. (beds.ac.uk)
  • Smooth-type LPS (S-LPS) from B. abortus demonstrated three broad high-molecular-weight bands corresponding to S-LPS, and a low-molecular-weight band corresponding to O antigen lacking rough-type LPS (R-LPS). (nih.gov)
  • This assay utilizes antigen from Brucella abortus strain W99. (mpbio.com)
  • The Brucella -specific production of gamma interferon (IFN-γ) in blood plasma produced by lymphocytes in antigen-stimulated whole-blood samples may be detected by indirect enzyme-linked immunosorbent assay (iELISA) ( 8 , 9 ). (asm.org)
  • Induction Murine Models of Chronic Fatigue Syndrome by Brucella abortus Antigen Injections: Is Anemia Induced or Not? (semanticscholar.org)
  • With the National Standard serum the titres to B. melitensis were consistently lower than those to B. abortus antigen. (up.ac.za)
  • Although this may be due to the vagaries of the test it also warrants closer investigation of the animals concerned to see whether M-antigen predominant Brucella biotypes are possibly present. (up.ac.za)
  • The use of the dual antigens could identify herds which are infected only with A-antigen predominant brucellae but would not be reliable for classifying individual animals. (up.ac.za)
  • Among Brucella immunogenes, antigen based on ribosomal preparation has been widely investigated. (ac.ir)
  • The Brucella Abortus Antigen Rapid Test Cassette (Whole Blood/Serum/Plasma) is a rapid chromatographic immunoassay for the qualitative detection of Brucella abortus antigen in human whole blood, serum or plasma specimen. (viho.es)
  • Brucella antigen in liver and spleen from Asian badgers infected with Brucella melitensis , northwestern China. (cdc.gov)
  • Probing the surface of bvrR and bvrS transposon mutants with monoclonal antibodies showed all described major outer membrane proteins (Omps) but Omp25, a protein known to be involved in Brucella virulence. (pnas.org)
  • So far, only one such system, the two-component regulatory system BvrR/BvrS, has been conclusively implicated in Brucella virulence ( 2 ). (pnas.org)
  • Cyclic β-1,2-glucans (CβG) are periplasmic homopolysaccharides that play an important role in the virulence and interaction of Brucella with the host. (asm.org)
  • IMPORTANCE In this study, we analyzed the interaction and localization of the proteins involved in the synthesis, transport, and modification of Brucella abortus cyclic β-1,2-glucans (CβG), which play an important role in the virulence and interaction of Brucella with the host. (asm.org)
  • Site-directed, unmarked deletion is an effective method for identifying virulence genes and constructing attenuated strains as Brucella vaccines. (biomedcentral.com)
  • Deletion of these genes reduces Brucella virulence, but they maintain excellent immunogenicity to activate the host immune response. (biomedcentral.com)
  • In a previous study, we identified a series of genes associated with B. abortus S2308 virulence using miniTn5 transposon mutagenesis (unpublished data). (biomedcentral.com)
  • The researchers have discovered a group of 24 genes that are linked to virulence by making comparisons of the newly available S19 genome sequence to previously sequenced genomes of two virulent strains of B. abortus . (vt.edu)
  • The paper "Genome sequence of Brucella abortus vaccine strain S19 compared to virulent strains yields candidate virulence genes" was published recently in PLoS One (May 2008, Volume 3, Issue 5, e2193). (vt.edu)
  • Oswald Crasta, project director at VBI's Cyberinfrastructure Group and the corresponding author on the paper, remarked: "We have been able to leverage rapid sequencing of the S19 genome on the Roche GS-20 TM and GS-FLX TM platforms, and comparative genomics narrowed down the search for Brucella virulence factors to a small group of genes. (vt.edu)
  • As a result, host responses generated during B. abortus infection are entirely dependent on detecting the deployment of a virulence factor, the type IV secretion system (T4SS), as a pathogen-induced process. (grantome.com)
  • In this application, we propose to study how this Brucella virulence factor induces the unfolded protein response as an immune signaling pathway. (grantome.com)
  • Immunization of rabbits with concentrated Brucella abortus antigens for 10 weeks led to nearly total loss of serum bactericidal activity for this organism. (jimmunol.org)
  • Serum samples from 20 animals (5.46 %) were positive for B. abortus by RBT and 1 (0.27 %) for B. canis by RSA. (scielo.org.za)
  • To evaluate the utility of these cards, 204 bovine blood serum samples from Brucella -infected and noninfected animals were stored on and eluted from the Protein Saver cards. (asm.org)
  • Anti- Brucella smooth lipopolysaccharide (sLPS) antibody titers for the serum eluates were compared to those of the unprocessed original serum samples by indirect enzyme-linked immunosorbent assay (ELISA). (asm.org)
  • Brucella -specific immune serum was raised in mice. (veterinaryworld.org)
  • Total B. abortus S544 counts in the spleen of each animal collected on the 7th day of challenge was determined to evaluate the protective index (PI) of anti- Brucella serum by statistical analysis. (veterinaryworld.org)
  • Human Brucella abortus IgM ELISA kit is designed for analysing in-vitro quantitative levels of human IgM antibodies to Brucella abortus in samples of serum and plasma. (elisakits.co.uk)
  • DNA of Brucella (B.) abortus was detected in serum of goats and sheep which has aborted recently by species-specific RT-PCR. (fu-berlin.de)
  • Recombinant EryC, Omp16, PP26, and PP39 were all reactive to B. abortus-positive serum. (mendeley.com)
  • To detect Brucella antigens, we used immunohistochemical staining of liver and spleen tissue sections by pipetting mouse anti- Brucella melitensis IgG diluted 1:100 in 30% bovine serum albumin/phosphate-buffered saline onto each section. (cdc.gov)
  • We tested putative Brucella colonies by using H 2 S production, dye inhibition, agglutination by monospecific serum, and sensitivity to bacteriophages ( Appendix Table). (cdc.gov)
  • Although bacterial isolation was used as reference, Brucella spp. (usp.br)
  • Moreover, cytokine production in DCs infected with B. abortus or stimulated with bacterial RNA was reduced upon pretreatment with MAPK/NF-κB inhibitors. (frontiersin.org)
  • We demonstrate that the downregulation of miR-125b-5p during B. abortus infection enhances the expression of the A20 protein, thereby inhibiting NF-kB activation, and facilitating bacterial intracellular survival. (biomedcentral.com)
  • The genus Brucella consists of 6 recognized bacterial species ( 1 ) and 2 proposed new species recently isolated from marine mammals ( 2 ). (aaccjnls.org)
  • The disease, caused primarily by the bacterial pathogens Brucella melitensis and Brucella abortus, is acquired by consumption of unpasteurized dairy products or by contact with infected animals. (grantome.com)
  • This study demonstrates the direct bactericidal effect of a high temperature (41ºC) on Brucella abortus that resulted in the gradual reduction of intracellular bacteria and inhibited bacterial growth within avian macrophage HD11 in an increasing period of time. (happyhaksul.com)
  • This study suggests the susceptibility of B. abortus to bacterial death under a high temperature with an increased period of incubation, leading to suppression of bacterial growth. (happyhaksul.com)
  • High within-herd prevalence, isolation of the pathogen and abortion confirm that B. abortus is circulating in this herd with cattle as reservoir hosts. (biomedcentral.com)
  • however, the importance and functionality of the Put system in the intracellular pathogen Brucellaabortus has not been explored. (microbiologyresearch.org)
  • The intracellular pathogen Brucella abortus survives and replicates preferentially in alternatively activated macrophages (AAM), however knowledge of the metabolic adaptations promoting exploitation of this niche is limited. (readbyqxmd.com)
  • The goal of this research program is to develop a mechanistic understanding of the general stress response (GSR) signaling system in the select agent pathogen, Brucella abortus. (grantome.com)
  • Brucella abortus is a stealthy pathogen expressing modified PAMPs that no longer serve as agonists for pattern recognition receptors. (grantome.com)
  • Brucella abortus is a stealthy pathogen that evades innate immunity, barely activates PMNs, and resists the killing mechanisms of these phagocytes. (una.ac.cr)
  • B. abortus causes abortion and infertility in adult cattle and is a zoonosis which is present worldwide. (wikipedia.org)
  • Cattle and other livestock are the major host species for the bacteria B. abortus. (wikipedia.org)
  • B. abortus can last a lot longer in animals if they aren't watched closely and if the cattle aren't getting treatment for it. (wikipedia.org)
  • In addition, infection may occur as the result of cattle ingesting B. abortus -contaminated feed or water. (idexx.com.au)
  • Phenotypic and genotypic characterisation of Brucella strains isolated from cattle in the Gambia. (ebscohost.com)
  • Holstein cattle of small scale dairy production systems were screened for Brucella abortus antibodies in 21 villages in Cameroon by ELISA. (up.ac.za)
  • A low seroprevalence in sheep and goats suggests a spillover of B. abortus from cattle to small ruminants in the herd. (biomedcentral.com)
  • Brucella abortus and B. melitensis antigens were used in parallel on the National Standard Brucella abortus antiserum and on field sera coming from cattle where practically exclusively B. abortus biotypes 1 and 2 have been isolated over the last 11 years. (up.ac.za)
  • Brucella abortus is a gram-negative bacterium that uses cattle and other ruminants as its primary host. (blogspot.com)
  • Scientists have long wanted to know what genetic features make strain S19 suitable for use as a vaccine in cattle because it may hold the secret as to why other Brucella strains cause disease and trigger the abortion of developing embryos in livestock. (vt.edu)
  • Some of the most common clinical signs of cattle being infected with B. abortus are retained placenta, high incidences of abortions and arthritic joints. (elisakits.co.uk)
  • We characterized 188 B. abortus isolates from CR recovered from cattle, humans and water buffalo, from 2003 to 2018, and whole genome sequencing (WGS) was performed in 95 of them. (cdc.gov)
  • The results suggest cross-species infection of B. abortus from cattle to non-preferred hosts raised in close contact. (fu-berlin.de)
  • The lipopolysaccharide (LPS) is considered the major virulent factor in Brucella spp. (ebscohost.com)
  • Usually, Brucella strains devoid of O-PS (rough mutants) are less virulent than the wild type. (ebscohost.com)
  • Verma S, Rawat M, Kumawat S, Qureshi S, Mohd G, Tiwari AK (2018) Protective role of Brucella abortus specific murine antibodies in inhibiting systemic proliferation of virulent strain 544 in mice and guinea pig, Veterinary World, 11(6): 794-799. (veterinaryworld.org)
  • The major objective of the investigation was to evaluate the hitherto uncharacterized potential of Brucella -specific antibodies to win the battle against virulent Brucella abortus infection. (veterinaryworld.org)
  • Virulent and attenuated Brucella abortus strains attach to and penetrate nonprofessional phagocytic HeLa cells. (pasteur.fr)
  • These mutants provide protection against wild-type, virulent Brucella challenge in mouse models [ 13 - 17 ], making them potential vaccine candidates. (biomedcentral.com)
  • These studies demonstrate that the B. abortus htrA cycL gene deletion mutant PHE1 is highly attenuated in the bovine host when compared to the virulent parental S2308. (lsu.edu)
  • In the present study, the eryC gene encoding B. abortus D-erythrulose 1-phosphate dehydrogenase, which is involved in the erythritol metabolism in virulent B. abort us strain but is absent from a B. abortus vaccine strain (S19), was cloned. (mendeley.com)
  • Brucella abortus is a Gram-negative facultative intracellular bacterium that causes disease in both humans and animals. (asm.org)
  • Brucella abortus is a facultative intracellular bacterium that causes chronic infection in animals and humans. (vt.edu)
  • Serology reagents intended to detect antibodies to Brucella abortus, a coccobacillus bacterium. (ophthalmologymanagement.com)
  • This project focuses on characterization of a conserved stress signaling system that controls survival of the pathogenic bacterium, Brucella abortus, inside its mammalian host. (grantome.com)
  • Two proteins encoded by BAB1_1794 and BAB2_0612 are highly over-produced in a Brucella abortus hfq mutant compared with the parental strain, and recently, expression of orthologues of these proteins in Agrobacterium tumefaciens was shown to be regulated by two sRNAs, called AbcR1 and AbcR2. (nih.gov)
  • Background: Lipopolysaccharides (LPSs) and several antigenic proteins of Brucella have been considered for preparation of diagnostic reagents and subunit vaccines. (ac.ir)
  • Furthermore several proteins of Brucella had similar reactions against all sera. (ac.ir)
  • A large number of hypothetical genes potentially encoding small proteins of unknown function are annotated in the Brucella abortus genome. (elsevier.com)
  • In this study, we observed by microarray analysis that several type I IFN-associated genes, such as IFN-β and guanylate-binding proteins (GBPs), are downregulated in STING knockout (KO) macrophages infected with Brucella or transfected with DNA. (readbyqxmd.com)
  • The proteins unique to biofilms and planktonic B. abortus were separated by two‑dimensional (2‑D) electrophoresis and then identified by matrix‑assisted laser desorption/ionization‑tandem time of flight‑mass spectrometry (MALDI‑TOF/TOF‑MS). High‑throughput sequencing and bioinformatic analyses were performed to identify differentially expressed genes between B. abortus cultured under biofilm and planktonic conditions. (spandidos-publications.com)
  • Here we report that while screening Brucella ORFeome for polar proteins in Brucella abortus , AidB was found to localize at the new pole, as well as at the constriction site in dividing cells. (biomedcentral.com)
  • Other B. abortus proteins, Omp16, PP26, and CP39 were also purified and their seroreactivities were compared. (mendeley.com)
  • A técnica de PCR, utilizando-se "primers" desenhados a partir do gene que codifica uma proteína imunogênica de 31 KDa de Brucella abortus , foi empregada na detecção de Brucella spp. (usp.br)
  • A PCR assay using primers designed based on the gene that encodes a 31 KDa immunogenic protein from Brucella abortus was used in the detection of Brucella spp. (usp.br)
  • Deletion of the BCSP31 gene of Brucella abortus by replacement. (asm.org)
  • The 31-kDa salt-extractable immunogenic protein, BCSP31, was deleted from several Brucella abortus strains by replacement with a marker gene encoding resistance to the antibiotics kanamycin and neomycin. (asm.org)
  • The BCSP31 gene replacement plasmids, constructed with ColE1-derived vectors, were introduced by electroporation into B. abortus strain 19 (S19), into a rough variant of B. abortus S19, and into B. abortus S2308, and antibiotic-resistant transformants were isolated. (asm.org)
  • In this study, the immunogenic ribosomal protein L7/L12 gene from Brucella abortus, S19, was amplified by PCR and sub-cloned to prokaryotic expression vector pET28a. (ac.ir)
  • and ( i ) an IS 711 element downstream of the bp26 gene in Brucella spp. (aaccjnls.org)
  • In this study, we constructed the Brucella abortus ( B. abortus ) S2308 mutant strain Δ22915, in which the putative lytic transglycosylase gene BAB_RS22915 was deleted. (biomedcentral.com)
  • The present study aimed to explore the differences in protein and gene expression of Brucella abortus cultured under biofilm and planktonic conditions. (spandidos-publications.com)
  • Moreover, a Brucella melitensis mutant in the alkA gene was found to be attenuated in mice (Pascal Lestrate, Ph.D. thesis, 2003). (biomedcentral.com)
  • We used the partial omp 22 gene (238 bp) encoding 22-kD outer membrane protein to identify the Brucella genus and the IS 711 gene to identify Brucella species. (cdc.gov)
  • The integrity of product was confirmed by Western-blot analysis using a standard rabbit anti Brucella abortus ribosomal protein L7/L12 antibody. (ac.ir)
  • Reports have shown that infection with B. abortus last for up to 42 days after abortion or parturition in vaginal discharges of infected cows 7 . (scielo.org.za)
  • We hypothesize that translocation of the T4SS substrate VceC activates the unfolded protein response (UPR) with consequent induction of NF-?B-dependent inflammatory responses, thereby contributing to B. abortus- induced abortion. (grantome.com)
  • This article will renew our knowledge about the Brucella agent causing abortion in small ruminants in Egypt. (fu-berlin.de)
  • Analysis of culture supernatants from viable B. abortus indicated that both GMP and adenine are produced by viable bacteria. (iastate.edu)
  • Brucella are small (0.5 to 0.7 by 0.6 to 1.5 μm), gram negative, non-motile, non- encapsulated, non-spore forming, rod shaped (coccobacilli) bacteria which are facultative intracellular parasites. (biomedcentral.com)
  • The majority of the strain 19 bacteria colocalized with the lysosome marker cathepsin D, suggesting that Brucella-containing phagosomes had fused with lysosomes, in which they may have degraded. (pasteur.fr)
  • Here, we demonstrate that the genes putA ( bab2_0518 ) and putR ( bab2_0517 ) are critical for the chronic infection of mice by B. abortus , but putA and putR are not required for the survival and replication of the bacteria in naive macrophages. (microbiologyresearch.org)
  • The genomes of two strains of B. abortus have been completely sequenced and these bacteria are somewhat unusual in having two separate chromosomes. (blogspot.com)
  • Brucella DNA is sensed by AIM2 and bacteria-induced mitochondrial reactiveoxygen species is detected by NLRP3. (conicet.gov.ar)
  • Nervous system invasion by bacteria of the genus Brucella results in an inflammatory disorder termedneurobrucellosis. (conicet.gov.ar)
  • Compared to pathogenic Brucella, the attenuated strain 19 hardly replicates within cells. (pasteur.fr)
  • Such analyses are subject to variable interpretations and must be performed by skilled technicians who are at high risk because most clinical Brucella strains are highly pathogenic. (aaccjnls.org)
  • Atluri VL, Xavier MN, de Jong MF, den Hartigh AB and Tsolis RM: Interactions of the human pathogenic Brucella species with their hosts. (spandidos-publications.com)
  • The assay, performed in flexible 96-well microplates coated with Brucella abortus antigens, utilizes 125 I-labeled staphylococcal protein A to detect antibody to Brucella abortus. (elsevier.com)
  • The Brucella abortus IgA ELISA Kit is intended for the measurement of human IgA antibodies to Brucella abortus. (mpbio.com)
  • A simple, rapid, inexpensive fluorescence polarization assay for the detection of antibodies to Brucella abortus in bulk tank milk samples at the farm level or at dairies with a sensitivity and specificity of 100 and 95.9%, respectively, is described. (asm.org)
  • The minimum detection sensitivity level of IgM antibodies to Brucella abortus using current human Brucella abortus IgM ELISA kit was 1.03 U/ml. (elisakits.co.uk)
  • Isolates of B. abortus, primarily. (ebscohost.com)
  • A total of 66 isolates of Brucella were recovered from sheep and yaks in the Inner Mongolia, Xinjiang, Qinghai and Gansu provinces of northwest China in 2015 and 2016. (readbyqxmd.com)
  • MLVA identified 98 unique B. abortus genotypes from disclosing isolates in the 387 herds involved in the epidemic. (cdc.gov)
  • Our results show circulation of five B. abortus lineages (I to V) in CR, phylogenetically related to isolates from the United States, United Kingdom, and South America. (cdc.gov)
  • A preliminary serological study of 366 household dogs in Lagos and Ibadan, southwestern Nigeria, was carried out to determine antibodies due to exposure to Brucella abortus and B. canis, using the rose bengal test (RBT) and the rapid slide agglutination (RSA) test, respectively. (scielo.org.za)
  • Results showed that 5.46 % (20/366) and 0.27 % (1/366) of the dogs screened were seropositive to B. abortus and B. canis, respectively. (scielo.org.za)
  • However, the isolation of B. canis from infected dogs has been reported 14 and serological reactions to B. abortus and B. canis documented 1,15 . (scielo.org.za)
  • The assays were performed using antigens prepared from B. abortus S99 and B. canis RM6/66 on each of the samples according to previously described methods 2,10 . (scielo.org.za)
  • In Brucella, abcR1 and abcR2 single mutants are not defective in their ability to survive in cultured murine macrophages, but an abcR1 abcR2 double mutant exhibits significant attenuation in macrophages. (nih.gov)
  • Additionally, expression of miR-125b-5p decreases A20 expression in B. abortus -infected macrophages and leads to NF-kB activation and increased production of TNFα. (biomedcentral.com)
  • Considering that Brucella infection induces significant changes in miRNAs expression in macrophages [ 7 ], we have investigated whether miRNAs also participate in the regulation of Brucella growth by targeting A20 expression. (biomedcentral.com)
  • In this study, we report that the miR-125b-5p-mediated regulation of A20 (TNFAIP3) plays an important role in tuning the activation of B. abortus -infected macrophages. (biomedcentral.com)
  • We report that AidB-YFP was systematically localized to the new poles and at constrictions sites in B. abortus , either in culture or inside infected HeLa cells or RAW264.7 macrophages. (biomedcentral.com)
  • This pattern of localization is maintained in B. abortus infecting epithelial cells and macrophages at different times post-infection. (biomedcentral.com)
  • Cortisol regulates target genes by binding to the glucocorticoid receptor (GR). B. abortus infection inhibited GRα expression. (frontiersin.org)
  • Signaling pathways triggered by Brucella abortus DNA involves TLR9, AIM2, and stimulator of IFN genes (STING). (readbyqxmd.com)
  • In addition to the conserved genes of Brucella , few novel regions showing similarity to the phages were identified in both strains. (jgenomics.com)
  • This work demonstrates that Brucella lipopolysaccharide (LPS) preparations are a family of related molecules which display heterogeneity not only at the level of the O polysaccharide, but also at the core oligosaccharide and the lipid A. Sodium dodecyl sulphate/polyacrylamide gel electrophoresis and Western blotting showed that LPS from Brucella strains displayed molecular weight and antigenic heterogeneity. (nih.gov)
  • Preparations of lipopolysaccharide (LPS) from rough and smooth strains of Brucella abortus were mitogenic for spleen cells of athymic nude mice, C3H/HeAU mice, and the endotoxin-resistant C3H/HeJ mice. (jimmunol.org)
  • Death of PMNs is concomitant with the intracellular Brucella lipopolysaccharide (Br-LPS) release within vacuoles. (una.ac.cr)
  • Abtahi H, Salmanian A H, Rafati S, Behzadian Nejad G, Mohammad Hassan Z. High Level Expression of Recombinant Ribosomal Protein (L7/L12) from Brucella abortus and Its Reaction with Infected Human Sera. (ac.ir)
  • Biochemical analyses demonstrated that a purified recombinant B. abortus PutA protein possesses quintessential proline dehydrogenase activity, as PutA is capable of catalysing the conversion of proline to glutamate. (microbiologyresearch.org)
  • Although B. abortus-infected glial cells secreted IL-1b and TNF-α, activation of HBMEC was dependent on IL-1b because CS from B. abortus-infected astrocytes and microglia deficient in caspase-1 and apoptosis-associated speck-like protein containing a CARD failed to induce HBMEC activation. (elsevier.com)
  • Protein synthesis in Brucella abortus induced during macrophage infection. (tamu.edu)
  • The small protein CydX is required for function of cytochrome bd oxidase in Brucella abortus. (elsevier.com)
  • The Brucella ORFeome, a collection of cloned predicted coding sequences, was placed in fusion with yellow fluorescent protein (YFP) coding sequence and screened for polar localizations in B. abortus . (biomedcentral.com)
  • In Escherichia coli , AidB protein takes part of the adaptative response to alkylating agents that could damage the genome [ 11 ], suggesting that AidB homolog could play a similar role in B. abortus . (biomedcentral.com)
  • Brucella has twelve different kinds of species, one being Brucella abortus. (wikipedia.org)
  • Prosthetic joint infection due to brucella species is rare. (nih.gov)
  • were confirmed as Brucella abortus by PCR using species-specific primers. (usp.br)
  • Brucella melitensis (biovars 1 and 3) is the predominant species, associated with sporadic cases and outbreak in humans. (ebscohost.com)
  • identify, characterize Brucella strains by Multiple Loci Variable Number of Tandem Repeats Analysis (MLVA-VNTR) and investigate possible spillover to other species. (biomedcentral.com)
  • Four members have recently been classified as additional species [ 10 , 11 ] and recently the eleventh Brucella spp. (biomedcentral.com)
  • Unique fragments occur among the different Brucella genomes, however, which allowed us to develop a rapid, specific single-test-tube assay for molecular identification of all known Brucella species. (aaccjnls.org)
  • Identification and differentiation of all Brucella species and vaccine strains by multiplex PCR. (aaccjnls.org)
  • Notably, this system is operative also in new strains/species of Brucella, among which Brucella microti, but not in the "classical" species, with the exception of marine mammals strains. (readbyqxmd.com)
  • There are many different ways B. abortus can spread from the different animals and even to humans. (wikipedia.org)
  • The brucellae are facultative intracellular parasites of animals and humans causing a disease of worldwide importance. (pnas.org)
  • This study aimed obtaining the data via Delphi Expert Opinion Surveys (DEOS) and Targeted Group Surveys (TGS) required for calculation of financial losses caused by Brucella abortus and Brucella melitensis in infected animals and humans and also required for performing cost-benefit analysis for. (ebscohost.com)
  • B. abortus is a significant zoonotic risk and animal health problem in this production system, therefore further studies on humans is recommended. (biomedcentral.com)
  • Brucella is able to pass from animals to humans with relative ease and poses a significant public health burden for workers in the livestock industry. (vt.edu)
  • Detection of IgM and IgG antibodies against Brucella by dot-ELISA in humans. (elisakits.co.uk)
  • Chemical characterization of B. abortus fractions 3b and 10 by HPLC and thin layer chromatography indicated that fraction 3b co-eluted and co-migrated with 5'-guanosine monophosphate (GMP) while fraction 10 co-eluted and co-migrated with adenine. (iastate.edu)
  • The assay detects antibodies to B. abortus in 15 min by testing undiluted whey produced by chemical and physical manipulation of milk from bulk tanks. (asm.org)
  • Temperature plays a huge role in the survival of B. abortus. (wikipedia.org)
  • Survival of B. abortus mutant strains in the macrophagelike cell line J774 and in HeLa cells was examined and shown to be indistinguishable from that of the parental strain. (asm.org)
  • Furthermore, B. abortus survival is attenuated in the presence of miR-125b-5p. (biomedcentral.com)
  • These results uncover a role for miR-125b-5p in the regulation of B. abortus intracellular survival via the control of A20 expression. (biomedcentral.com)
  • Nevertheless, to our knowledge, there has been no research to indicate that a specific host miRNA regulates Brucella intracellular survival. (biomedcentral.com)
  • Brucella abortus induces an inflammatory response that stimulates the endocrine system resulting in the secretion of cortisol and dehydroepiandrosterone (DHEA). (frontiersin.org)
  • B. abortus infection induces apoptosis and inhibits osteoblast function. (frontiersin.org)
  • The seroprevalence to B. abortus by the Rose Bengal test was 12% (16/133), these positive reactors were confirmed by competitive ELISA and a prevalence of 3% (4/16) was established. (scielo.org.co)
  • This is the first survey in the area of Lorica in the department of Córdoba which has established a seroprevalence to B. abortus from 3% in water buffalo population of livestock in this region of the country. (scielo.org.co)
  • This study aimed at investigating a dairy herd experiencing abortions in order to establish within-herd seroprevalence to Brucella spp. (biomedcentral.com)
  • Pathological, immunohistochemical and bacteriological study of tissues and milk of cows and fetuses experimentally infected with Brucella abortus. (semanticscholar.org)
  • Additionally, the abcR1 abcR2 double mutant displays significant attenuation in a mouse model of chronic Brucella infection. (nih.gov)
  • Therefore, mutant strain ∆22915 could be used as a novel vaccine candidate in the future to protect animals against B. abortus infection. (biomedcentral.com)
  • A B. abortus cydX mutant lacked cytochrome bd oxidase activity, as shown by increased sensitivity to H(2)O(2), decreased acid tolerance and increased resistance to killing by respiratory inhibitors. (elsevier.com)
  • To investigate cell-type dependent invasion of B. abortus, phagocytic RAW 264.7 and THP-1 cells and non-phagocytic HeLa cells were infected with wild-type and mutant B. abortus, and their invasion efficiencies were compared. (bvsalud.org)
  • Accordingly, a B. abortus aidB mutant is more sensitive than the wild-type strain to the lethality induced by methanesulphonic acid ethyl ester (EMS). (biomedcentral.com)
  • Brucella abortus enters phagocytes that invade human and animal innate defenses which in turn, cause chronic disease in the host. (wikipedia.org)
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  • These studies suggest that fractions 3b and 10 are GMP and adenine respectively and that these materials contribute to the inhibition of intracellular destruction of B. abortus by PMNs. (iastate.edu)
  • Standardization of the technique enabled the differentiation of all Brucella spp. (usp.br)
  • DHEA treatment reversed the effect of B. abortus infection on osteoblast by increasing their proliferation, inhibiting osteoblast apoptosis, and reversing the inhibitory effect of B. abortus on osteoblast differentiation and function. (frontiersin.org)
  • Culture supernatants (CS) from glial cells (astrocytes and microglia) infected with B. abortus also induced activation of HBMEC, but to a greater extent. (elsevier.com)
  • Herein, we discuss the mechanism of caspase-1 activation and IL-1β secretion in glial cells infected with B. abortus.Our results demonstrate that the ASC inflammasome is indispensable for inducing the activation of caspase-1 and secretion of IL-1β upon infection of astrocytes and microglia with Brucella. (conicet.gov.ar)
  • We homogenized spleen samples of badgers and the 14 aborted sheep fetuses and inoculated these homogenates onto individual Brucella agar plates, which we then incubated at 37°C in an atmosphere of 5% CO 2 for 5 days. (cdc.gov)
  • Brucellae are intracellular pathogens that have the ability to survive and multiply inside professional phagocytic cells. (biomedcentral.com)
  • Wild-type B. abortus invasion efficiency was greater in phagocytic cells than in epithelial cells. (bvsalud.org)
  • The results also indicated that there are different factors involved in the invasion of B. abortus into phagocytic cells. (bvsalud.org)
  • The impact of cross-reactivity in serological assays leading to FPSRs may be reduced by additionally measuring the cellular immune response to Brucella antigens. (asm.org)
  • A specific additional 1320-bp fragment was amplified, and the 450-bp fragment was absent in Brucella strains isolated from marine mammals. (aaccjnls.org)
  • The aim of this study was to estimate the prevalence of Brucella abortus in buffaloes ( Bubalus bubalis ) from three farms in the town of Lorica in the department of Cordoba. (scielo.org.co)