Brucella abortus: A species of the genus BRUCELLA whose natural hosts are cattle and other bovidae. Abortion and placentitis are frequently produced in the pregnant animal. Other mammals, including humans, may be infected.Brucella: A genus of gram-negative, aerobic bacteria that causes BRUCELLOSIS. Its cells are nonmotile coccobacilli and are animal parasites and pathogens. The bacterium is transmissible to humans through contact with infected dairy products or tissue.Brucellosis: Infection caused by bacteria of the genus BRUCELLA mainly involving the MONONUCLEAR PHAGOCYTE SYSTEM. This condition is characterized by fever, weakness, malaise, and weight loss.Brucellosis, Bovine: A disease of cattle caused by bacteria of the genus BRUCELLA leading to abortion in late pregnancy. BRUCELLA ABORTUS is the primary infective agent.Brucella Vaccine: A bacterial vaccine for the prevention of brucellosis in man and animal. Brucella abortus vaccine is used for the immunization of cattle, sheep, and goats.Brucella melitensis: A species of the genus BRUCELLA whose natural hosts are sheep and goats. Other mammals, including humans, may be infected. In general, these organisms tend to be more virulent for laboratory animals than BRUCELLA ABORTUS and may cause fatal infections.Brucella ovis: A species of the genus BRUCELLA which are pathogenic to SHEEP.Brucella canis: A species of gram-negative bacteria infecting DOGS, the natural hosts, and causing canine BRUCELLOSIS. It can also cause a mild infection in humans.Abortion, Veterinary: Premature expulsion of the FETUS in animals.Antibodies, Bacterial: Immunoglobulins produced in a response to BACTERIAL ANTIGENS.Agglutination Tests: Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (From Stedman, 26th ed)Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Erythritol: A four-carbon sugar that is found in algae, fungi, and lichens. It is twice as sweet as sucrose and can be used as a coronary vasodilator.Chlamydophila: A genus of the family CHLAMYDIACEAE comprising gram-negative non CHLAMYDIA TRACHOMATIS-like species infecting vertebrates. Chlamydophila do not produce detectable quantities of glycogen. The type species is CHLAMYDOPHILA PSITTACI.Bison: A genus of the family Bovidae having two species: B. bison and B. bonasus. This concept is differentiated from BUFFALOES, which refers to Bubalus arnee and Syncerus caffer.Antigens, Bacterial: Substances elaborated by bacteria that have antigenic activity.Brucella suis: A species of gram-negative bacteria, primarily infecting SWINE, but it can also infect humans, DOGS, and HARES.Complement Fixation Tests: Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.Ochrobactrum anthropi: A species of gram-negative, obligately aerobic rods. Motility occurs by peritrichous flagella. (From Bergey's Manual of Determinative Bacteriology, 9th ed)Bacterial Vaccines: Suspensions of attenuated or killed bacteria administered for the prevention or treatment of infectious bacterial disease.Mice, Inbred BALB CBacterial Proteins: Proteins found in any species of bacterium.Virulence: The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.Spleen: An encapsulated lymphatic organ through which venous blood filters.Rose Bengal: A bright bluish pink compound that has been used as a dye, biological stain, and diagnostic aid.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Macrophages: The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)Buffaloes: Ruminants of the family Bovidae consisting of Bubalus arnee and Syncerus caffer. This concept is differentiated from BISON, which refers to Bison bison and Bison bonasus.Lipopolysaccharides: Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed)Bacterial Outer Membrane Proteins: Proteins isolated from the outer membrane of Gram-negative bacteria.Vaccination: Administration of vaccines to stimulate the host's immune response. This includes any preparation intended for active immunological prophylaxis.Sheep Diseases: Diseases of domestic and mountain sheep of the genus Ovis.Hydroxybenzoates: Benzoate derivatives substituted by one or more hydroxy groups in any position on the benzene ring.Goats: Any of numerous agile, hollow-horned RUMINANTS of the genus Capra, in the family Bovidae, closely related to the SHEEP.Prosthesis-Related Infections: Infections resulting from the implantation of prosthetic devices. The infections may be acquired from intraoperative contamination (early) or hematogenously acquired from other sites (late).Joint Prosthesis: Prostheses used to partially or totally replace a human or animal joint. (from UMDNS, 1999)PubMed: A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.Oxo-Acid-Lyases: Enzymes that catalyze the cleavage of a carbon-carbon bond of a 3-hydroxy acid. (Dorland, 28th ed) EC 4.1.3.Mycoplasma synoviae: A species of gram-negative bacteria pathogenic to CHICKENS; TURKEYS, and guinea fowls. It causes disease in a wide variety of organs and tissues including JOINTS, tendon sheaths and the RESPIRATORY TRACT.Genome, Bacterial: The genetic complement of a BACTERIA as represented in its DNA.Dehydroepiandrosterone: A major C19 steroid produced by the ADRENAL CORTEX. It is also produced in small quantities in the TESTIS and the OVARY. Dehydroepiandrosterone (DHEA) can be converted to TESTOSTERONE; ANDROSTENEDIONE; ESTRADIOL; and ESTRONE. Most of DHEA is sulfated (DEHYDROEPIANDROSTERONE SULFATE) before secretion.Osteoblasts: Bone-forming cells which secrete an EXTRACELLULAR MATRIX. HYDROXYAPATITE crystals are then deposited into the matrix to form bone.Cytokines: Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.Prions: Small proteinaceous infectious particles which resist inactivation by procedures that modify NUCLEIC ACIDS and contain an abnormal isoform of a cellular protein which is a major and necessary component. The abnormal (scrapie) isoform is PrPSc (PRPSC PROTEINS) and the cellular isoform PrPC (PRPC PROTEINS). The primary amino acid sequence of the two isoforms is identical. Human diseases caused by prions include CREUTZFELDT-JAKOB SYNDROME; GERSTMANN-STRAUSSLER SYNDROME; and INSOMNIA, FATAL FAMILIAL.PrPC Proteins: Normal cellular isoform of prion proteins (PRIONS) encoded by a chromosomal gene and found in normal and scrapie-infected brain tissue, and other normal tissue. PrPC are protease-sensitive proteins whose function is unknown. Posttranslational modification of PrPC into PrPSC leads to infectivity.Prion Diseases: A group of genetic, infectious, or sporadic degenerative human and animal nervous system disorders associated with abnormal PRIONS. These diseases are characterized by conversion of the normal prion protein to an abnormal configuration via a post-translational process. In humans, these conditions generally feature DEMENTIA; ATAXIA; and a fatal outcome. Pathologic features include a spongiform encephalopathy without evidence of inflammation. The older literature occasionally refers to these as unconventional SLOW VIRUS DISEASES. (From Proc Natl Acad Sci USA 1998 Nov 10;95(23):13363-83)PrPSc Proteins: Abnormal isoform of prion proteins (PRIONS) resulting from a posttranslational modification of the cellular prion protein (PRPC PROTEINS). PrPSc are disease-specific proteins seen in certain human and animal neurodegenerative diseases (PRION DISEASES).Peyer's Patches: Lymphoid tissue on the mucosa of the small intestine.Scrapie: A fatal disease of the nervous system in sheep and goats, characterized by pruritus, debility, and locomotor incoordination. It is caused by proteinaceous infectious particles called PRIONS.Nigeria: A republic in western Africa, south of NIGER between BENIN and CAMEROON. Its capital is Abuja.Dog Diseases: Diseases of the domestic dog (Canis familiaris). This term does not include diseases of wild dogs, WOLVES; FOXES; and other Canidae for which the heading CARNIVORA is used.Dogs: The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)

Modulation of endocytosis in nuclear factor IL-6(-/-) macrophages is responsible for a high susceptibility to intracellular bacterial infection. (1/780)

Activated macrophages kill bacteria, a function known to depend on the expression of NF-IL-6. Here, it is demonstrated that the attenuated Brucella abortus vaccine strain 19 replicates much better in NF-IL-6-/- than in NF-IL-6(+/+) and NF-IL-6(+/+)-activated murine macrophages and at levels comparable to those observed in normal macrophages infected with the pathogenic strain 2308. The role of NF-IL-6 in the inhibition of intracellular bacterial replication is related to its control of endocytosis and membrane fusion between endosomes and Brucella-containing phagosomes. Addition of the granulocyte-CSF (G-CSF), whose induction is impaired in NF-IL-6(-/-) macrophages, restores both endocytosis and the morphology of endosomes, together with bactericidal activity. Regulation of membrane traffic in endocytosis by G-CSF whose expression is controlled by NF-IL-6 may explain how a host cell can control intracellular bacterial replication.  (+info)

Immunity to Brucella in mice vaccinated with a fraction (F8) or a killed vaccine (H38) with or without adjuvant. Level and duration of immunity in relation to dose of vaccine, recall injection and age of mice. (2/780)

Immunity to Brucella in the mouse, assessed by bacterial spleen counts 15 days after intraperitoneal inoculation of a standard challenge of B. abortus 544, has been studied with two vaccines, one experimental, composed of a fraction of the bacterial cell-wall (F8) extracted from B. abortus 99, the other of killed whole bacteria, B. melitensis 53 H38, taken as reference (H38). The level of primary immunity depended on the dose of vaccine, the presence of oil adjuvant and the age of the mouse. The presence of adjuvant enabled the immunization to F8 to continue beyond the first month, to reach its maximum around the fourth month, and to remain stable for at least 7 months. A booster injection 3 or 6 months after the primary vaccination reinforced existing immunity but did not increase it beyond a certain level. The effect of the recall injection was clearly demonstrated with low doses which gave a lower level of primary immunity.  (+info)

Murine macrophage-lymphocyte interactions: scanning electron microscopic study. (3/780)

Light and scanning electron microscopic observations revealed murine macrophage-lymphocyte interactions involving the initial contact of peritoneal, spleen, or thymus lymphocytes with peritoneal macrophage processes or microprocesses followed by clustering of lymphocytes over the central nuclear area of the macrophages. Lymphocyte-lymphocyte clustering was not observed in the absence of macrophages. Attachment and subsequent clustering appeared not to require the presence of serum or antigen; the attachment of allogeneic or xenogeneic lymphocytes was comparable to that seen in the syngeneic system, but central clustering of these lymphocytes failed to occur. No attachment or clustering was observed when thymic lymphocytes were cultured with thymus derived fibroblasts rather than with peritoneal macrophages. Lymphocyte attachment to immune, antigen-activated, syngeneic macrophages occurred more rapidly than that to normal unstimulated syngeneic macrophages; however, lymphocytes attached to the "activated" macrophages appeared to be killed by a nonphagocytic mechanism. A similar increase in the rate of lymphocyte attachment to macrophages occurred in the presence of migration inhibitory factor. Subsequent lymphocyte clustering on macrophages was observed in the migration inhibitory factor-stimulated cultures. In addition, lymphocyte-macrophage interactions similar to those in vitro were observed to occur in vivo on intraperitoneally implanted cover slips.  (+info)

Plaque-forming cells in mice after experimental infection with Brucella abortus. (4/780)

Cells producing antibody to brucella lipopolysaccharide were detected in spleens of mice infected with Brucella abortus 19 by a hemolytic plaque assay. The appearance of immunoglobulin M-producing cells preceded humoral antibodies. The primary plaques were observed 5 days after inoculation, and they were still present by day 70.  (+info)

Antibody response to antigens distinct from smooth lipopolysaccharide complex in Brucella infection. (5/780)

The smooth lipopolysaccharide complex of the outer surface of smooth Brucella abortus cells is believed to be the antigenic component involved in serological tests routinely used for the diagnosis of brucellosis. Sera from cattle vaccinated or infected with B. abortus generally contain antibody directed toward the smooth lipopolysaccharide complex. The brucella organism contains a large number of other antigenically distinct components. The biological significance of some of these antigens has been demonstrated by showing that sera from infected cattle have precipitins to these components. These sera revealed up to seven distinct lines in immunoelectrophoresis with a protein-rich antigen mixture prepared from rough strain B. abortus 45/20, whereas sera from strain 19-vaccinated cattle did not reveal these lines at 4 or more months after vaccination. Monospecific antisera were prepared against six antigens in this mixture, and the purification of two of them by antibody affinity chromatography is described.  (+info)

The siderophore 2,3-dihydroxybenzoic acid is not required for virulence of Brucella abortus in BALB/c mice. (6/780)

2,3-Dihydroxybenzoic acid (DHBA) is the only siderophore described for Brucella, and previous studies suggested that DHBA might contribute to the capacity of these organisms to persist in host macrophages. Employing an isogenic siderophore mutant (DeltaentC) constructed from virulent Brucella abortus 2308, however, we found that production of DHBA is not required for replication in cultured murine macrophages or for the establishment and maintenance of chronic infection in the BALB/c mouse model.  (+info)

Structure and function prediction of the Brucella abortus P39 protein by comparative modeling with marginal sequence similarities. (7/780)

A methodology is proposed to solve a difficult modeling problem related to the recently sequenced P39 protein. This sequence shares no similarity with any known 3D structure, but a fold is proposed by several threading tools. The difficulty in aligning the target sequence on one of the proposed template structures is overcome by combining the results of several available prediction methods and by refining a rational consensus between them. In silico validation of the obtained model and a preliminary cross-check with experimental features allow us to state that this borderline prediction is at least reasonable. This model raises relevant hypotheses on the main structural features of the protein and allows the design of site-directed mutations. Knowing the genetic context of the P39 reading frame, we are now able to suggest a function for the P39 protein: it would act as a periplasmic substrate-binding protein.  (+info)

Comparison of sensitivity and specificity of purified lymphocyte and whole-blood in vitro lymphocyte stimulation assays in detection of Brucella abortus infection in cattle. (8/780)

A study was conducted to compare the sensitivity and specificity of purified lymphocyte and whole-blood in vitro lymphocyte stimulation assays in detection of Brucella abortus infection in cattle. Cattle used were infected with B. abortus field strains or strain 19. Peripheral blood was collected, and lymphocytes for the technique. The blood for the whole-blood lymphocyte stimulation assay was diluted 10-fold with RPMI 1640 medium (without additional serum supplement) and cultured. The two tests were run simultaneously, and B. abortus soluble antigen or concanavalin A was added to the cultures. The cultures were incubated for 6 days and assayed for [3H] thymidine incorporation into their DNA. Generally, cultures of the purified lymphocyte stimulation assay had higher counts per minute than those of the whole-blood lymphocyte stimulation assay, but the stimulation ratios for the two tests were comparable. The two assays were comparable in terms of their sensitivity and specificity as applied to detection of brucella infection in cattle.  (+info)

The Brucella abortus strain S19, a spontaneously attenuated strain, has been used as a vaccine strain in vaccination of cattle against brucellosis for six decades. Despite many studies, the physiological and molecular mechanisms causing the attenuation are not known. We have applied pyrosequencing technology together with conventional sequencing to rapidly and comprehensively determine the complete genome sequence of the attenuated Brucella abortus vaccine strain S19. The main goal of this study is to identify candidate virulence genes by systematic comparative analysis of the attenuated strain with the published genome sequences of two virulent and closely related strains of B. abortus, 9-941 and 2308. The two S19 chromosomes are 2,122,487 and 1,161,449 bp in length. A total of 3062 genes were identified and annotated. Pairwise and reciprocal genome comparisons resulted in a total of 263 genes that were non-identical between the S19 genome and any of the two virulent strains. Amongst these, 45 genes
Brucella abortus is a facultative intracellular bacterial pathogen, which causes abortion in cattle and undulant fever in human. B. abortus strain RB51 (Strain RB51) is the official vaccine for bovine brucellosis in the USA. B. abortus strain RB51 can be used as a vector for the over-expression of its own (homologous) as well as heterologous protective antigens. The immune system can detect these heterologous antigens and produce a response. Expressing a protein in different bacterial compartments has been shown to affect its accessibility to the immune system and the way the antigen is processed by antigen presenting cells. In order to determine if the immune response is affected by the localization of the antigen, green fluorescent protein (GFP) was expressed at three different locations in B. abortus strain RB51, outer-membrane (OM), periplasmic space (PS) and in the cytoplasmic region (CR) of B. abortus strain RB51. This localization was obtained by transforming strain RB51 with plasmids ...
Abstract. We report the draft genome sequences of two Brucella abortus strains LMN1 and LMN2 isolated from cattle and pig. The LMN1 and LMN2 have the genome size of 3,395,952 bp and 3,334,792 bp, respectively. In addition to the conserved genes of Brucella, few novel regions showing similarity to the phages were identified in both strains.. Keywords: genome sequence, Brucella abortus ...
Brucella abortus induces an inflammatory response that stimulates the endocrine system resulting in the secretion of cortisol and dehydroepiandrosterone (DHEA). Osteoarticular brucellosis is the most common presentation of the active disease in humans and we have previously demonstrated that B. abortus infection inhibits osteoblast function. We aimed to evaluate the role of cortisol and DHEA on osteoblast during B. abortus infection. B. abortus infection induces apoptosis and inhibits osteoblast function. DHEA treatment reversed the effect of B. abortus infection on osteoblast by increasing their proliferation, inhibiting osteoblast apoptosis and reversing the inhibitory effect of B. abortus on osteoblast differentiation and function. In contrast, cortisol increased the effect of B. abortus infection. Cortisol regulates target genes by binding to the glucocorticoid receptor (GR). B. abortus infection inhibited GR-α expression. Cells responses to cortisol not only depend on GR expression but also on its
Brucella abortus is a Gram-negative, facultative intracellular bacterium that causes brucellosis, a worldwide zoonotic disease leading to undulant fever in humans and abortion in cattle. The immune response against this bacterium relies on the recognition of microbial pathogen-associated molecular patterns, such as lipoproteins, lipopolysaccharides and DNA; however, the immunostimulatory potential of B. abortus RNA remains to be elucidated. Here, we show that dendritic cells (DCs) produce significant amounts of IL-12, IL-6 and IP-10/CXCL10, when stimulated with purified B. abortus RNA. IL-12 secretion by DCs stimulated with RNA depends on TLR7 while IL-6 depends on TLR7 and partially on TLR3. Further, only TLR7 plays a role in IL-12 production induced by B. abortus infection. Moreover, cytokine production in DCs infected with B. abortus or stimulated with bacterial RNA was reduced upon pretreatment with MAPK/NF-κB inhibitors. By confocal microscopy, we demonstrated that TLR7 is colocalized with B.
Dendogram of global Brucella abortus biovar 1 genotypes. Dendogram based on MLVA-16 genotyping showing the relationship of 49 B. abortus biovar 1 strains origin
Brucella abortus is a facultative intracellular bacterial pathogen, which causes abortion in cattle and undulant fever in human. B. abortus strain RB51 (Strain RB51) is the official vaccine for bovine brucellosis in the USA. B. abortus strain RB51 can be used as a vector for the over-expression of its own (homologous) as well as heterologous protective antigens. The immune system can detect these heterologous antigens and produce a response. Expressing a protein in different bacterial compartments has been shown to affect its accessibility to the immune system and the way the antigen is processed by antigen presenting cells. In order to determine if the immune response is affected by the localization of the antigen, green fluorescent protein (GFP) was expressed at three different locations in B. abortus strain RB51, outer-membrane (OM), periplasmic space (PS) and in the cytoplasmic region (CR) of B. abortus strain RB51. This localization was obtained by transforming strain RB51 with plasmids ...
Brucella abortus is a Gram-negative bacterium that causes abortion and infertility in food animals and a chronic debilitating febrile disease in humans known as brucellosis. As with all pathogenic bacteria, the Brucella spp. require sufficient metal nutrition during the course of an infection. Host-mediated metal withdrawal defenses actively restrict the bioavailability of metals which requires invading bacteria to employ high affinity metal acquisition systems to overcome these metal-limiting conditions. While obtaining sufficient metals during host infection is critical to the survival of these bacteria, avoiding metal toxicity is equally important. Excess accumulation of one metal relative to others can lead to protein mis-metallation when surplus metal ions outcompete other metal species for their native binding sites. To prevent metal toxicity, bacteria respond to high intracellular metal concentrations by means of metal-responsive transcriptional regulators that downregulate metal import ...
Background: Lipopolysaccharides (LPSs) and several antigenic proteins of Brucella have been considered for preparation of diagnostic reagents and subunit vaccines. The objective of this study was to identify and compare immunogens of B. abortus S19 which induce humoral immune responses in human, goat and rabbit. Material and Methods: The bacterial whole cell extract was prepared in extraction buffer and resolved using two-dimensional gel electrophoresis (2-DE). The resolved antigens were reacted against human, goat and rabbit sera using western blotting. Results: At least 19, 14 and 16 immunogenic proteins were recognized in western blotting with human, goat and rabbit sera, respectively. The most abundant proteins of the bacterium with immunogenic properties in goat and rabbit but not in human, were a group of 5-6 proteins with molecular masses of 32-34 KDa and isoelectric point (pI) ranging from 4.5 to 5.7. In contrast, a group of 5 proteins with molecular weight of 45 KDa and pI in the range of 4.5
A serological survey of Brucella abortus in cattle originating from communal grazing areas of Kwa Zulu Natal was carried out between March 2001 and December 2003. The survey was designed as a 2-stage survey, considering the diptank as the primary sampling unit. In total 46 025 animals from 446 diptanks of 33 magisterial districts were sampled and tested using the Rose Bengal test and Complement Fixation Test. The apparent prevalence at district level was adjusted for clustering, diagnostic test sensitivity and specificity, and mapped using ArcView version 3.3. The prevalence of brucellosis in communal grazing areas of Kwa-Zulu Natal was found to be 1.45 % (0.84-2.21 %) and varied from 0 to 15.6% between magisterial districts. In 19 of the 33 magisterial districts no serological reactors were observed. A large variation in prevalence was found within diptank areas. Brucellosis was found to be most prevalent in the northeastern area of the province. The findings of the survey are discussed ...
In this study, we explore the regulatory roles of pro-inflammatory cytokine tumor necrosis factor alpha (TNF) in the innate immunity of macrophages against B. abortus infection. We show that infection of macrophage with B. abortus induces marked expression and secretion of TNF which subsequently binds to TNF receptor 1 (TNFR-1) and activates a downstream signaling cascade of the innate immunity. Blocking of TNF signaling resulted in a notable increase of B. abortus survival which was associated with an increase of anti-inflammatory cytokine interleukin 10 (IL-10), a beneficial effector of Brucella survival, as well as remarkable decrease of reactive oxygen species (ROS) and nitric oxide (NO), antibrucella molecules ...
Converts alpha-N-acetylneuranimic acid (Neu5Ac) to the beta-anomer, accelerating the equilibrium between the alpha- and beta-anomers. Probably facilitates sialidase-negative bacteria to compete sucessfully for limited amounts of extracellular Neu5Ac, which is likely taken up in the beta-anomer. In addition, the rapid removal of sialic acid from solution might be advantageous to the bacterium to damp down host responses.
Entry of the facultative intracellular pathogen Brucella into host cells results in the formation of endosomal Brucella-containing vacuoles (eBCVs) that initially traffic along the endocytic pathway. eBCV acidification triggers the expression of a type IV secretion system that translocates bacterial effector proteins into host cells. This interferes with lysosomal fusion of eBCVs and supports their maturation to replicative Brucella-containing vacuoles (rBCVs). Bacteria replicate in rBCVs to large numbers, eventually occupying most of the cytoplasmic volume. As rBCV membranes tightly wrap each individual bacterium, they are constantly being expanded and remodeled during exponential bacterial growth. rBCVs are known to carry endoplasmic reticulum (ER) markers, however, the relationship of the vacuole to the genuine ER has remained elusive. We have reconstructed the 3-dimensional ultrastructure of rBCVs and associated ER by correlative structured illumination microscopy (SIM) and focused ion ...
Ubiquinone (UQ), also called coenzyme Q, and plastoquinone (PQ) are electron carriers in oxidative phosphorylation and photosynthesis, respectively. The quinoid nucleus of ubiquinone is derived from the shikimate pathway; 4-hydroxybenzoate is directly formed from chorismate in bacteria, while it can be formed from either chorismate or tyrosine in yeast. The following biosynthesis of terpenoid moiety involves reactions of prenylation, decarboxylation, and three hydroxylations alternating with three methylations. The order of these reactions are somewhat different between bacteria and yeast. Phylloquinone (vitamin K1), menaquinone (vitamin K2), and tocopherol (vitamin E) are fat-soluble vitamins. Phylloquinone is a compound present in all photosynthetic plants serving as a cofactor for photosystem I-mediated electron transport. Menaquinone is an obligatory component of the electron-transfer pathway in bacteria ...
Brucella abortus causes brucellosis mainly in cattle. The infection is transmitted to humans by ingestion of animal products or direct contact with infected material. While the intracellular lifestyle of Brucella is well characterized, its extracellular survival is poorly understood. In nature, bacterial persistence is associated with biofilms, where aggregated cells are protected from adversity. The inability of Brucella abortus to aggregate under aerobiosis and that fact that the replicative niche of Brucella is characterized by microaerobic conditions prompted us to investigate the capacity of this pathogen to aggregate and grow in biofilms under microaerobiotic conditions. The results show that B. abortus aggregates and produces biofilms. The aggregates tolerate desiccation better than planktonic cells do, adhere and displace even in the absence of the lipopolysaccharide-O antigen, flagella, the transcriptional regulator VjbR, or the enzymes that synthesize, transport, and modify cyclic β ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
1. Targonski P.V, Jacobson R.M, Poland G.A. Immunosenescence: role and measurement in influenza vaccine response among the elderly. Vaccine. 2007;25:3066-3069 2. Noreddin A.M, Haynes V. Use of pharmacodynamic principles to optimise dosage regimens for antibacterial agents in the elderly. Drugs Aging. 2007;24:275-292 3. Liang S.Y, Mackowiak P.A. Infections in the elderly. Clin Geriatr Med. 2007;23:441-456 4. Vasto S, Candore G, Balistreri C.R, Caruso M, Colonna-Romano G. et al. Inflammatory networks in ageing, age-related diseases and longevity. Mech Ageing Dev. 2007;128:83-91 5. High K.P, Prasad R, Marion C.R, Schurig G.G, Boyle S.M. et al. Outcome and immune responses after Brucella abortus infection in young adult and aged mice. Biogerontology. 2007;8:583-593 6. Htwe T.H, Mushtaq A, Robinson S.B, Rosher R.B, Khardori N. Infection in the elderly. Infect Dis Clin North Am. 2007;21:711-743 7. Hakim F.T, Gress R.E. Immunosenescence: deficits in adaptive immunity in the elderly. Tissue Antigens. ...
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The innate immune system detects the presence of microbes in tissue by pattern recognition of conserved microbial structures, known as pathogen-associated molec...
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Brucella BvrR protein: BvrR/BvrS represent the two-component system involved in the regulation of Brucella virulence; amino acid sequence in first source
Very little is known about the role of DNA repair networks in Brucella abortus and its role in pathogenesis. We investigated the roles of RecA protein DNA repair and SOS regulation in B. abortus. While recA mutants in most bacterial species are hyper ...
Luo et al., 2006a: Luo DY, Li P, Xing L, Zhao GY, Shi W, Zhang SL, Wang XL. DNA vaccine encoding L7/L12-P39 of Brucella abortus induces protective immunity in BALB/c mice. Chinese medical journal. 2006 Feb 20; 119(4); 331-4. [PubMed: 16537031 ...
Size, charge and structural heterogeneity of Brucella abortus lipopolysaccharides demonstrated by two-dimensional gel electrophoresis (pages 283-288). Dr. Blair A. Sowa, Richard P. Crawforda, Fred C. Heck, John D. Williams, Albert M. Wu, Katherine A. Kelly and L. Garry Adams. Version of Record online: 16 APR 2008 , DOI: 10.1002/elps.1150070608. ...
Bacillus anthracis is a facultative intracellular bacterial pathogen that can cause cutaneous, gastrointestinal or respiratory disease in many vertebrates, including humans. Commercially available anthrax vaccines for immunization of humans are of limited duration and do not protect against the respiratory form of the disease. Brucella abortus is a facultative intracellular bacterium that causes chronic infection in animals and humans. As with other intracellular pathogens, cell mediated immune responses (CMI) are crucial in affording protection against brucellosis. B. abortus strain RB51 has been shown to be useful in eliciting protective cell mediated immunity and humoral responses against Brucella in cattle and other animal species. Since the protective antigen (PA) of B. anthracis is known to induce protective antibodies, it was decided that the objective of this research was to test whether the gene encoding PA could be expressed in Brucella producing a bivalent vaccine to protect against ...
The roles of humoral and cell mediated immune responses in murine brucellosis were investigated in this study.B. abortus strain 19, the current vaccine strain, is known to induce an antibody as well as cell mediated immune responses, both of which protect mice against smooth strain 2308. B. abortus rough strain RB51 does not induce an o-side chain specific antibody response and yet protects mice against smooth strain 2308. Passive transfer experiments using serum and nylon wool enriched T cells obtained from micevaccinated with strain 19 and strain RB51 were carried out. Immune senum from strain 19 vaccinated mice protected against challenge with strain 2308 but not strain RB51. Nylon wool enriched T cells from strain 19 vaccinated mice protected recipient mice against challenge with both strains RB51 or 2308. Serum obtained from RB51 vaccinated mice did not protect recipient mice against challenge with either strain RB51 or strain 2308. Nylon wool enriched T cells from the same vaccinated mice, ...
Accepted name: D-erythritol 1-phosphate dehydrogenase. Reaction: D-erythritol 1-phosphate + NADP+ = D-erythrulose 1-phosphate + NADPH + H+. Other name(s): eryB (gene name). Systematic name: D-erythritol-1-phosphate 2-oxidoreductase. Comments: The enzyme, characterized from the pathogenic bacterium Brucella abortus, which causes brucellosis in livestock, participates in erythritol catabolism.. Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: References:. 1. Sperry, J.F. and Robertson, D.C. Erythritol catabolism by Brucella abortus. J. Bacteriol. 121 (1975) 619-630. [PMID: 163226]. 2. Sangari, F.J., Aguero, J. and Garcia-Lobo, J.M. The genes for erythritol catabolism are organized as an inducible operon in Brucella abortus. Microbiology 146 (2000) 487-495. [PMID: 10708387]. 3. Barbier, T., Collard, F., Zuniga-Ripa, A., Moriyon, I., Godard, T., Becker, J., Wittmann, C., Van Schaftingen, E. and Letesson, J.J. Erythritol feeds the pentose phosphate pathway via three new ...
DO NOT REPLY TO THIS MESSAGE DIRECTLY PLEASE VISIT http://www.medcor.mcgill.ca/EXPMED/DOCS/jobs.html New on ExpMed Job Listings (Post Doc: fluorescence polarization assays (FPA) for the diagnosis of Infectious Disease Min Lin, Ph.D. Animal Diseases Research Institute Immunology Nepean, Ontario, Canada Project description: A post-doctoral position is available immediately to carry out research that leads to development of homogeneous fluorescence polarization assays (FPA) for the diagnosis of infectious diseases. The studies may include identification and characterization of the immunodominant protein/carbohydrate antigens from pathogenic bacteria, molecular cloning, protein expression/purification, epitope mapping, chemical modification of proteins, structure/function analysis of proteins, and assessment of the immune response to antigens. Recent publications: (1)Lin, M., and K. Nielsen. 1997. Binding of the Brucella abortus lipopolysaccharide O-chain fragment to a monoclonal antibody. J. Biol. ...
Looking for online definition of abortus in the Medical Dictionary? abortus explanation free. What is abortus? Meaning of abortus medical term. What does abortus mean?
The results of our study are in agreement with the reports of other workers1,3,15,17, who also demonstrated the presence of B. abortus agglutinins in dog sera using RBT. The higher prevalence of 5.46 % recorded by RBT compared to 0.27 % (only 1 animal) by RSA could be attributed to the practice of feeding dogs with foetuses from slaughtered cows or meat at abattoirs as the prevalence of brucellosis is 6 % in cattle in Nigeria5.. Although not conclusive, our data suggest that young adult dogs (,1 year ) are most affected (Table 1). This is corroborated by a report of higher rates in this age group1and a study that suggested that Brucella infection in dogs is age-dependent15. In addition, we recorded higher prevalences in females (Table 2) but in another study a slightly higher rate in males (29.6 %) than in females (26.7 %) was recorded1. A contributing factor to higher rates in females could be that a single male dog, if infected and used to mate with several females, can transmit the infection ...
Brucellosis is a disease that can be prevented through vaccination. Yet, the effectiveness of the vaccination to fight this disease is considered weak. Fortunately, attempts to modify brucellosis vaccine is still keep going. Some brucellosis vaccines have been found and developed in the past time such as the vaccine B.abortus strain 19-BA and 104M which was made from weakened microbes which had been widely used in Uni Soviet and China. The other brucellosis vaccine that were used in the past were the phenolinsoluble peptidoglycan vaccine which was made in France and polysaccharide-protein vaccine which was used in Russia. This research attempted to see the determinant of antigenic Outer Membrane Protein (OM) 36 kDa Brucella abortus local isolation which has immunogenic character to be developed as an advanced brucellosis vaccine. The method used in this research was the Omp2 gene of Brucella abortus of local isolate employed the PCR technique. The result of the PCR was then sequenced to analyze ...
The enzyme, characterized from the pathogenic bacterium Brucella abortus, which causes brucellosis in livestock, participates in erythritol catabolism.
An anti-Brucella vaccine candidate which comprises of purified Brucella lipopolysaccharide and cocktail of four Salmonella Typhimurium (ST)-Brucella vectors was reported earlier. Each vector constitutively express highly conserved Brucella antigens (rB), viz. lumazine synthase (BLS), proline racemase subunit A (PrpA), outer membrane protein-19 (Omp19), and Cu-Zn superoxide dismutase (SOD). The present study determined relative level of protection conferred by each single strain. Upon virulent challenge, the challenge strain was recovered most abundantly in non-immunized control mice and the ST-Omp19, ST-BLS, LPS, and ST-SOD immunized mice groups showed much less burden. Indirect ELISA based assay also confirmed the induction of antigen specific IgG for each antigen delivered. In route-wise comparison of the combined vaccine candidate, intraperitoneal (IP), intramuscular (IM), and subcutaneous (SC) immunizations revealed an indication of highly efficient routes for the protection. Splenocytes of ...
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While macrophages are normally considered to be a primary cell for mediating innate immune responses by phagocytosing and destroying microbes that infect mammals, some microbial organisms including bacteria and protozoa have specifically adapted themselves to survive and replicate in host cells thereby "hiding" from protective immune responses. We study Brucella abortus, a bacterium that causes disease in a wide variety of agricultural animals and in people, to evaluate how some animals but not others successfully prevent infections by intracellular pathogens. We are particularly interested in the role of host cytokines such as interleukins and gamma-interferon produced by T lymphocytes in controlling the infection through their ability to activate macrophages for antimicrobial activities. In addition we are interested in identifying genes of the bacteria that facilitate their survival in host macrophages thus allowing them to establish chronic infections. Our second major area of research ...
As debate on the merits of mandatory pasteurization of milk intended for cheesemaking continues, global demand for raw-milk cheese is increasing.. While the Food and Drug Administration is conducting a scientific evaluation on the microbiological safety of cheeses made from raw milk, such as Parmigiano Reggiano, Gruyere, Gouda, and Cheddar, it may be useful to review some of the risk assessments and policy discussions that are occurring around the globe.. United States regulations governing the use of raw milk for cheese-making, promulgated in 1949, require cheesemakers to either pasteurize raw milk or hold cheese at no lower than 35°F for a minimum of 60 days. Mycobacterium tuberculosis and Brucella abortus were the pathogens of concern at that time, and aging allowed pathogen inactivation.. As a result of improvements in animal health and disease management, these are no longer primary pathogens of concern. However, Escherichia coli O157:H7, Salmonella typhimurium DT 104, and Listeria ...
Abortus technieken en methoden Noodanticonceptie: Hormonale methoden door inname van hoge dosissen oestrogenen: Klassieke noodpil (of Yuzpe-methode) of morning-afterpil (MAP), Levonorgestrel (LNG) (progestogeen-enkelpil, noodpil, morning-afterpil), Mifepristone of abortuspil (RU486) - Mechanische Methode: Abortus tijdens het 1e trimester van de zwangerschap: Abortuspil, Vacu maspiratie, Het embryo wordt opgezogen en vervolgens in delen opgevangen, Dilatatie en Curettage. Abortus tijdens het 2e trimester van de zwangerschap: Prostaglandinen, (Te) vroege keizersnede of ...
The primers for the genes of the identified proteins were designed and amplificated by PCR in four Brucella strains. The optimized PCR conditions and the length of PCR products were determined. The results of the PCR amplification in four different Brucella strains were shown." +", Corresponding sequence have been able to amplify; "-", Corresponding sequence not been able to amplify ...
When and how often laboratory tests are done may depend on many factors. The timing of laboratory tests may rely on the results or completion of other tests, procedures, or treatments. Lab tests may be performed immediately in an emergency, or tests may be delayed as a condition is treated or monitored. A test may be suggested or become necessary when certain signs or symptoms appear. Due to changes in the way your body naturally functions through the course of a day, lab tests may need to be performed at a certain time of day. If you have prepared for a test by changing your food or fluid intake, lab tests may be timed in accordance with those changes. Timing of tests may be based on increased and decreased levels of medications, drugs or other substances in the body. The age or gender of the person being tested may affect when and how often a lab test is required. Chronic or progressive conditions may need ongoing monitoring through the use of lab tests. Conditions that worsen and improve may ...
Eksploration: uterus st rrelse, lejring og adnexae vurderes. En evt. 2 klot nger i forreste portiol be. Dilatation til hegar -1 over sugets st rrelse. Sugets st rrelse svarer til gestationsalderen. Suget indf res til fundus uden vacuum. Vacuum tilsluttes og kaviteten t mmes. Der g s efter med stump curette, specielt i hj rnerne. Minimal bl dning og en velkontraheret uterus tyder p , at kaviteten er tom. Det kontrolleres ved abdominal ultralydsscanning, at kaviteten er tom, ligesom ultralydsscanning kan anvendes ved indf ring af suget, hvis der er i tvivl om uteri lejring. Afvaskning med klorhexidin 0,05% mindsker ikke infektionsraten ...
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Kang, Yoon-Suk, Daniel A. Brown, and James E. Kirby. "Brucella neotomae Recapitulates Attributes of Zoonotic Human Disease in a Murine Infection Model." Infection and Immunity 87.1 (2019): e00255-18. Web. 08 April. 2020. ...
Prion protein is the causative agent of the transmissible spongiform encephalopathies. According to the "prion hypothesis," the infectious isoform of prion protein, termed PrPSc, replicates by interacting with cellular PrPC and mediating its conformational change into the disease-causing PrPSc (19). Compared with its well-defined pathological significance, the physiological function of PrPC remains unclear. PrPC is highly expressed not only by cells in the CNS but also by follicular DCs, mature myeloid cells, and activated T cells. This distribution suggests involvement of PrPC in immune surveillance (20).. Our present study defines a novel role for PrPC as an M-cell receptor for the uptake of pathogenic bacteria. PrPC on macrophages has been reported to recognize surface-exposed Hsp60 of B. abortus and to facilitate internalization of the bacteria (13); however, Fontes et al. (21) reported a contradictory result. Using Prnp−/− mice, they showed that B. suis infection is independent of PrPC ...
Selectins, are known to be increased in the serum of patients with pre-eclampsia, indicating that these molecules are possible markers of endothelial cell injury. In this study, we investigated P,Eand Lselectin levels in normal pregnancy,pre-eclampsia, and missed abortus. Plasma P and L selectins levels were significantly higher in normal pregnancy and pre-eclampsia than healthy controls; but plasma concentrations of E selectins were not different between these groups. Plasma P selectin was significantly higher in pre-eclampsia than normal pregnancy. Plasma concentrations of all selectins were significantly higher in missed abortus than healthy control. L selectin levels were higher in pre-eclampsia and missed abortus than normal pregnancy. We found the levels of selectins were increased in pre-eclampsia and missed abortus. Although selectins were suspected to play a role in the pathogenesis of pre-eclampsia, in conjunction with previous studies, we thought that elevated selectin levels are a non
To analyze the strategy adopted by Brucella to develop within mononuclear cells, we examined whether Brucellainfection was able to positively or negatively modulate apoptosis in human monocytic phagocytes. The results of various analyses (DNA fragmentation, microscopic analysis, and flow cytometry analysis) showed that Brucella infection inhibited apoptosis which spontaneously occurs in human monocytes in the absence of an activation signal and rendered macrophagic cells resistant to apoptosis induced by immunological factors.. Although the Brucella-mediated effect on monocyte apoptosis was somewhat similar to the E. coli LPS effect (references24 and 28 and our results), convergent data demonstrated that Brucella LPS cannot account for the antiapoptotic properties of the live bacteria. (i) Experiments performed in the presence of neutralizing anti-TNF-α Ab showed that TNF-α, which plays a central role in Brucella(or E. coli) LPS-induced protection of monocytes, did not participate in the ...
Looking for online definition of brucellae in the Medical Dictionary? brucellae explanation free. What is brucellae? Meaning of brucellae medical term. What does brucellae mean?
ALBANY, N.Y. (AP) - Health officials have issued a warning about potentially tainted raw milk that was sold in New York and other states.. The Journal News reports the Centers for Disease Control and Prevention is advising anyone who consumed Udder Milk products to seek medical attention. According to the CDC, people who drank raw milk from the company may have been infected with a rare germ called Brucella abortus RB51.. Officials say a New Jersey woman became sick with the bacteria after consuming Udder Milk earlier this year.. Authorities say Udder Milk operates in New York, New Jersey, Connecticut and Rhode Island. Officials describe the company as a "co-op on wheels" that makes deliveries.. Authorities are investigating Udder Milks supplier because the company hasnt reported its dairy source.. ___. Information from: The Journal News, http://www.lohud.com. ...
Objective To study antimicrobial resistance of Brucella melitenesis and instruct rational use of antimicrobial agents for clinic.Methods Bacteria were cultured and identified by Bact/Alert 3D and VITEK Ⅱ automicrobic system.Drug susceptibility was detected by E-test method.Results Four strains Brucella melitenesis were isolated from blood of 4 patients with fever and MIC of gentamicin,ampicillin and ciprofloxacin was(0.5)~(0.75 mg/L),(1.5)~(2.0 mg/L) and (4.0)~(8.0 mg/L) respectively.MIC of ceftazidime,amikacin and doxycycline were all above(8.0 mg/L).Conclusion The preferred drug for treating brucellosis is gentamicin or plus ampicillin or ciprofloxacin,or choosing antimicrobial agents according to antimicrobial susceptibility test results.
LPS from S. abortus equi (S-form) Biotin TLRpure Sterile Solution (Lipopolysaccharide), TLRpure and ultrapure (|99.9%). Isolated from S. abortus equi. Used for Innate Immunity, Adjuvant, Inflammasome, Autophagy and Signal Transduction Research.
Pourquier, P. (IDvet, France), Rodalakis, A and Mohamad, KY (INRA, Nouzilly, France). Preliminary validation of a new commercial ELISA kit for the detection of antibodies directed against C. abortus. Presented at the WAVLD Conference, 2007 ...
This test looks for brucellosis, an infectious disease usually caused by handling animals or milk products infected with the brucella bacteria.
This test looks for brucellosis, an infectious disease usually caused by handling animals or milk products infected with the brucella bacteria.
LOCUS SBW13224.1 61 aa PRT BCT 14-DEC-2016 DEFINITION Brucella sp. 10RB9215 hypothetical protein protein. ACCESSION LT599047-28 PROTEIN_ID SBW13224.1 SOURCE Brucella sp. 10RB9215 ORGANISM Brucella sp. 10RB9215 Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Brucellaceae; Brucella; unclassified Brucella. REFERENCE 1 AUTHORS Blom J. JOURNAL Submitted (05-JUL-2016) to the INSDC. JLU, JLU, Justus-Liebig-University Giessen, Bioinformatics and Systems Biology, Heinrich-Buff-Ring 58, 35392 Giessen, 35390, Germany FEATURES Qualifiers source /organism=Brucella sp. 10RB9215 /chromosome=1 /host=African bull frog, Pyxicephalus edulis /isolate=BR10RB9215WGS1 /mol_type=genomic DNA /collection_date=2009 /db_xref=taxon:1149953 protein /transl_table=11 /locus_tag=BR10RB9215_C10028 /db_xref=UniProtKB/TrEMBL:A0A1M4L950 BEGIN 1 MEGQVIFYKN DRIIYRHHID VQEDDYSKGV NDALIAFQRN YAGFDLASDD IHIRFKKPGD 61 V ...
PCR-RFLP analysis of Brucella LPS genes manAO-Ag, manBO-Ag, wbkD, wbkF, wboA and wa**. Panel A. Lanes: 1, molecular size markers; 2, manAO-Ag from B. melitensis
See chapter 12 for further information. (357) This disease is caused by the bacterium Brucella suis. Infection of the pregnant sow may result in abortion. Generally it...
Ten bottlenose dolphins that died in the Gulf of Mexico recently were found to have been infected with the bacteria Brucella. They are among the nearly 600 dolphins that have died in the Gulf since February 2010. Due to the high number of deaths, NOAA has declared an â unusual mortality event,â triggering a focused, expert investigation into the cause.
SPAIN – After 20 years of eradication efforts, four autonomous areas have been officially declared bovine Brucellosis free.
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Spleen cells from mice infected with the rough Brucella melitensis strain B115 were fused with NSO myeloma cells. Hybridoma supernatants were screened in ELISA with cell walls (CW), sonicated cell extracts (CE) and rough lipopolysaccharide (R-LPS) of B. melitensis strain B115 and whole B. melitensis B115 cells. Surprisingly, 22 monoclonal antibodies (mAbs) reacting in ELISA with both CW and CE but not with R-LPS and bacterial cells were shown by immunoblot analysis and ELISA to react with smooth lipopolysaccharide (S-LPS). These mAbs also reacted in ELISA with O polysaccharides (OPS) from the smooth Brucella abortus strain 99 and the smooth B. melitensis strain 16M and thus recognize epitopes present on the O-chain. Proteinase K LPS preparations from B. melitensis B115 analysed by immunoblotting with one mAb (12G12) recognizing S-LPS of both A and M specificity displayed the typical S-LPS high-molecular-mass ladder pattern but no S-LPS was detected in the phenol/water/chloroform/light petroleum LPS
The genus Brucella contains highly infectious species that are classified as biological threat agents. The timely detection and identification of the microorganism involved is essential for an effective response not only to biological warfare attacks but also to natural outbreaks. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) is a rapid method for the analysis of biological samples. The advantages of this method, compared to conventional techniques, are rapidity, cost-effectiveness, accuracy and suitability for the high-throughput identification of bacteria. Discrepancies between taxonomy and genetic relatedness on the species and biovar level complicate the development of detection and identification assays. In this study, the accurate identification of Brucella species using MALDI-TOF-MS was achieved by constructing a Brucella reference library based on multilocus variable-number tandem repeat analysis (MLVA) data. By comparing MS-spectra from Brucella
Curated}} {{Biorealm Genus}} [[Image:brucella_close_up.JPG,thumb,400px,right,Electron micrograph of B. abortus located inside cisternae of Vero cell. Within the perinuclear envelope, the cisternae containing B. abortus are discontinuously lined by ribosomes (arrows). From [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2114362 Detilleux et al.]]] ==Classification== ===Higher order taxa:=== Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Brucellaceae ===Species:=== Brucella abortus, Brucella canis, Brucella cetaceae, Brucella maris , Brucella melitensis, Brucella pinnipediae, Brucella sp. {, , height="10" bgcolor="#FFDF95" align="center" , NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=234 Taxonomy] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=genomeprj&cmd=Search&dopt=DocSum&term=txid234%5BOrganism:exp%5D Genomes] ,} ==Description and Significance== Brucella sp. causes brucellosis, ...
Using serology and bacterial culture, we determined the prevalence of Brucella spp. and the antibody to Brucella spp. in a feral swine (Sus scrofa) population in proximity to a cattle herd that was culture positive for Brucella abortus and Brucella suis in north-central Texas, USA. During a prospective cross-sectional quantitative study in April 2005, we collected blood and tissue samples from 40 feral swine within a 30-km radius of the infected herd. Serum samples were tested by the Rose Bengal test, particle concentration fluorescence immunoassay, and fluorescence polarization assay. In addition, tissue samples were cultured, and the Brucella species and biovar determined. Four feral swine were Brucella positive by serology, and two were culture positive for B. suis biovar 1. Of the culture-positive swine, one was concurrently antibody and culture positive, and one was culture positive only. The presumptive source of the B. suis infection in the index cattle herd was likely the surrounding feral swine
1. Chronic infections may have negative impacts on wildlife populations, yet their effects are difficult to detect in the absence of long-term population monitoring. Brucella abortus, the bacteria responsible for bovine brucellosis, causes chronic infections and abortions in wild and domestic ungulates, but its impact on population dynamics is not well understood. 2. We report infection patterns and fitness correlates of bovine brucellosis in African buffalo based on (1) 7 years of cross-sectional disease surveys and (2) a 4-year longitudinal study in Kruger National Park (KNP), South Africa. We then used a matrix population model to translate these observed patterns into predicted population-level effects. 3. Annual brucellosis seroprevalence ranged from 8·7% (95% CI = 1·8-15·6) to 47·6% (95% CI = 35·1-60·1) increased with age until adulthood (,6) and varied by location within KNP. Animals were on average in worse condition after testing positive for brucellosis (F = −5·074, P , ...
Pathogens infecting mammalian cells have developed various strategies to suppress and evade their hosts defensive mechanisms. In this line, the intracellular bacteria that are able to survive and propagate within their host cells must have developed strategies to avert their hosts killing attitude. Studying the interface of host-pathogen confrontation can provide valuable information for defining therapeutic approaches. Brucellosis, caused by the Brucella strains, is a zoonotic bacterial disease that affects thousands of humansand animals around the world inflicting discomfort and huge economic losses. Similar to many other intracellular dwelling bacteria, infections caused by Brucella are difficult to treat, and hence any attempt at identifying new and common therapeutic targets would prove beneficial for the purpose of curing infections caused by the intracellular bacteria. In THP-1 macrophage infected with Brucella melitensis we studied the expression levels of four hosts genes, i.e. EMP2, ST8SIA4
ABSTRACT - EPIDEMIOLOGICAL EVALUATION OF A VACCINATION CAMPAIGN AGAINST BOVINE BRUCELLOSIS IN THE REGION OF ALENTEJO WITH THE RB51 VACCINE - Bovine Brucellosis (BB) is a bacterial disease of cattle caused by Brucella abortus, less frequently by B. melitensis and occasionally by B. suis, which can cause disease in humans. It is a notifiable disease with worldwide distribution, responsible for economic losses in countries such as Portugal, where it is endemic. Clinically it is characterized by one or more of the following clinical signs: abortion in the last third of gestation, retained placenta, orchitis, epididymitis and, rarely, arthritis. Infection can occur to offspring in utero or by contact with secretions and excretions of infected animals, with high rates of infectivity and resistance in the environment. The clinical diagnosis does not allow the confirmation of the infection, being necessary to use the laboratory diagnosis. To track the infection, serology tests are often used. Is common ...
Isolation, amplification, and detection of DNA and RNA sequences in "molecular diagnostic" devices are at the forefront of modern diagnostic medicine. Such technologies offer unprecedented sensitivity and specificity in the detection of infectious disease. Molecular diagnostics are typically very expensive, large, and require a modern laboratory and trained technicians to operate---greatly restricting the use of molecular diagnostic tools in the developing world.. DFA is building paper-based technology to perform molecular diagnostics on an extremely inexpensive, disposable device. DFA is currently developing a nucleic acid amplification-based paper-microfluidic device for early infant diagnosis of HIV under a Saving Lives at Birth grant. Prior test kit development included a DARPA-funded effort to identify E. coli and a DTRA-funded effort with Harvard University focused on Brucella abortus. We believe our approach represents a fundamental shift in the field of nucleic acid detection that can ...
In 1999, a researcher suffered headaches, lassitude and a severe sinusitis after exposure to marine mammal strains of Brucella with which the worker was in contact. The symptoms resolved in one week after treatment with doxycycline and rifampin. The researcher had a positive titer for Brucella, and the organism was cultured from blood samples. PCR-RFLP was used to positively identify the isolates as being comparable to marine mammal Brucella (Brew and Staunton 1999). Organisms Brucella ssp. are gram-negative intracellular bacteria and are a major source of zoonoses worldwide. B. melitensus, B. abortus, and B. suis are some species commonly recognized to play a role in human and animal health. Nomenclature for marine mammal strains of Brucella has not yet been fully developed, but B. maris and B. pinnipedia have so far been named. Transmission occurs primarily through contact with aborted fetal material, and consumption of contaminated milk. ...
Accepted name: alanine racemase. Reaction: L-alanine = D-alanine. Other name(s): L-alanine racemase. Systematic name: alanine racemase. Comments: A pyridoxal-phosphate protein.. Links to other databases: BRENDA, EXPASY, GTD, KEGG, Metacyc, PDB, CAS registry number: 9024-06-0. References:. 1. Marr, A.G. and Wilson, P.W. The alanine racemase of Brucella abortus. Arch. Biochem. Biophys. 49 (1954) 424-433.. 2. Wood, W.A. Amino acid racemases. Methods Enzymol. 2 (1955) 212-217.. 3. Wood, W.A. and Gunsalus, I.C. D-Alanine formation: a racemase in Streptococcus faecalis. J. Biol. Chem. 190 (1951) 403-416.. ...
Mapping the restriction fragments of the Brucella melitensis 16M genome with a new restriction endonuclease, PacI, which cut the DNA into only eight fragments, indicated that this species contains two unique and independent replicons of about 2,100 and 1,150 kb. Pulsed-field gel electrophoresis of intact DNA revealed two bands migrating the expected distances. These replicons were identified as two unique and independent chromosomes by the presence of rRNA operons and genes for heat shock proteins mapping to separate replicons. ...
Interpretive Summary: Brucella are intracellular pathogens that cause reproductive losses in cattle and zoonotic infections in people. Live attenuated vaccines have been available for protecting domestic livestock against brucellosis for more than 60 years. Current vaccines are effective in preventing abortion and transmission of brucellosis, but poor at preventing infection or seroconversion. Current knowledge suggests that both the innate and adaptive immune responses contribute to immunity against intracellular pathogens and that binding of pathogen structures onto pattern recognition receptors (PMRs) is critical to development of protective immunity. The observations that killed bacteria or subunit vaccines do not appear to fully stimulate PMRs or mimic intracellular trafficking of live Brucella, may explain their inability to induce protection that equals that provided by live vaccines. Brucella appear to have multiple mechanisms that subvert innate and adaptive immunity and prevent or ...
... is an infectious disease caused the Brucella bacteria which spread to humans from animals. Some Brucella bacteria strains are seen in cows, while some occur in pigs, dogs, sheep, camels and goats. This is the forum for discussing anything related to this health condition
Brucellosis is a zoonosis or a disease that can be transmitted from animals to humans. People can also get the disease by eating products from infected animals. It is also known as undulant fever or Mediterranean fever.
FUN_05: Use the SSGCID solved structure of Brucella melitensis methionine-tRNA-synthetase (MetRS) to find inhibitors for potential drug development for brucellosis.
U bent nu zwanger … dagen (… weken en … dagen).. Een medische abortus werkt het beste wanneer de medicatie zo vroeg mogelijk in de zwangerschap wordt genomen en ze zijn meest effectief tot 63 dagen (9 weken) van de zwangerschap.. ...
The current investigation is associated with the third known case of brucellosis from Brucella RB51 in a New York resident who who drank raw milk purchased from Millers Biodiversity Farm in Quarryville, Pennsylvania, was diagnosed with brucellosis in November 2018.. Milk samples from the dairy tested positive for Brucella strain RB51. People who consumed raw milk or raw milk products from this dairy since January 2016 may have been exposed.. As of January 22, 2019, exposures have been identified in 19 states: Alabama, California, Connecticut, Florida, Georgia, Iowa, Maryland, Massachusetts, Michigan, Minnesota, Mississippi, New Jersey, New York, North Carolina, Ohio, Pennsylvania, Rhode Island, South Carolina, and Virginia.. CDC notes that Brucella strain RB51 is a live-attenuated cattle vaccine strain, which can be shed in milk and can cause infections in humans. RB51 is resistant to rifampin and penicillin. There is no serological test available to detect RB51 infection. Blood culture is the ...
Brucellosis is a contagious disease of animals that also affects humans. The disease is also known as Bangs Disease. In humans, its known as Undulant Fever.. Brucellosis is one of the most serious diseases of livestock, considering the damage done by the infection in animals. Decreased milk production, weight loss, loss of young, infertility, and lameness are some of the affects on animals.. The Brucella species are named for their primary hosts: Brucella melitensis is found mostly is goats,sheep and camels, B. abortus is a pathogen of cattle, B. suis is found primarily in swine and B. canis is found in dogs.. There are two common ways people get infected with brucellosis. First, individuals that work with infected animals that have not been vaccinated against brucellosis. This would include farmers, slaughterhouse workers and veterinarians.. Related: College microbiology classes linked to Salmonella Typhimurium outbreak. They get infected through direct contact or aerosols produced by the ...
We present a rare case of brucella endocarditis, forming a vegetation on the mitral valve. The definitive diagnosis has been made with clinical suspicion, positive serology, the demonstration of the vegetation with the echocardiography and with the production from the multiple blood culture of brucella melitensis and from the excised valve. Our patient has been successfully treated with specific antibiotherapy and the surgery of replacement of mitral valve. Our aim in presenting the case is to remind the infective endocarditis which is due to this factor in the regions like our country which is endemic for ...
What is brucellosis? Brucellosis is an infectious disease caused by the bacteria of the genus Brucella. These bacteria are primarily passed among animals, and they cause disease in many different vertebrates. Various Brucella species affect sheep, goats, cattle, deer, elk, pigs, dogs, and several other animals. Humans become infected by coming in contact with animals or animal products that are contaminated with these bacteria. In humans brucellosis can cause a range of symptoms that are similar to the flu and may include fever, sweats, headaches, back pains, and physical weakness. Severe infections of the central nervous systems or lining of the heart may occur. Brucellosis can also cause long-lasting or chronic symptoms that include recurrent fevers, joint pain, and fatigue.. Back to Top How common is brucellosis? Brucellosis is not very common in the United States, where100 to 200 cases occur each year. But brucellosis can be very common in countries where animal disease control programs have ...
Isolated case reports of peritonitis due to Brucella spp. during peritoneal dialysis (PD) continue to surface in the medical literature. However, the optimal treatment regimen for these patients, in particular with regards to the fate of PD catheter, is still largely unknown. We report a case of brucella peritonitis successfully treated with intraperitoneal administration of amikacin, along with oral rifampicin and doxycycline but without catheter removal. Furthermore, we have reviewed the literature up until present day.
Brucellosis is a worldwide zoonotic disease transmitted to humans by consumption of contaminated milk and milk products. Brucellosis is endemic in Turkey, and Edirne has a high Brucella prevalence. Brucellosis is prevented by live-attenuated vaccines for animals and the vaccination program has been in place since 1984 in Turkey. Thrace is the pilot region for this vaccination program. The gold standard diagnostic technique for brucellosis is still the isolation of suspicious bacterial colonies followed by bacteriological identification, but it is very time consuming and laborious. In many studies, Brucella has been investigated by PCR techniques. However, PCR-based methods cannot differentiate between the vaccine strain and the virulent strain; thus, the vaccine strain may interfere with the virulent strain and causes false-positive reactions. To monitor brucellosis control programs effectively, it is important to distinguish vaccine and field strains of Brucella spp. In this study, raw milk ...
Among animals, brucellosis is transmitted directly (contact with placenta, aborted fetus, the liquor , vaginal and/or uterus secretion) or indirectly (if infected and non-infected health herds mix while grazing or by contact with environment in which the bacteria can be found such as manure, pastures, water, etc). Most infected male animals continue to secrete Brucella via semen for the lifetime. Presence of infectious agents in udder and surrounding lymph nodes results in permanent or occasional secretion of Brucella via milk and as such it is a serious source of infection for young animals which can also be infected via uterus. In infected lambs and young goats, Brucella inhabits lymph nods of bowls and can be secreted out of their body via droppings. Infected young cattle develop the disease once they reached sexual maturity. Sheppard dogs can also spread brucellosis between ruminants but they can also get ill as well. In high humidly, low temperature and unsunny weather conditions, Brucella ...
ID BRUMB_1_PE1003 STANDARD; PRT; 44 AA. AC BRUMB_1_PE1003; C0RJ20; DT 00-JAN-0000 (Rel. 1, Created) DT 00-JAN-0000 (Rel. 2, Last sequence update) DT 00-JAN-0000 (Rel. 3, Last annotation update) DE SubName: Full=Putative uncharacterized protein; (BRUMB_1.PE1003). GN OrderedLocusNames=BMEA_A1086; OS BRUCELLA MELITENSIS ATCC 23457. OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Brucellaceae; OC Brucella. OX NCBI_TaxID=546272; RN [0] RP -.; RG -.; RL -.; CC -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS BRUMB_1.PE1003. CC Brucella melitensis ATCC 23457 chromosome chromosome I, complete sequen CC sequence. CC -!- ANNOTATIONS ORIGIN:C0RJ20_BRUMB CC -!- GENE_FAMILY: HBG000000000 [ FAMILY / ALN / TREE ] DR UniProtKB/Swiss-Prot; C0RJ20; -. DR EMBL; CP001488; ACO00828.1; -; Genomic_DNA. DR RefSeq; YP_002732782.1; NC_012441.1. DR GeneID; 7677846; -. DR GenomeReviews; CP001488_GR; BMEA_A1086. DR KEGG; bmi:BMEA_A1086; -. DR ProtClustDB; CLSK2476470; -. DR HOGENOMDNA; BRUMB_1.PE1003; -. ...
Brucellosis, Undulant fever, Mediterranean fever or Malta fever is different wards for one disease which is an infectious disease caused by a type of bacteria called Brucella , that are almost invariably transmitted by direct or indirect contact with infected animals or their products. Additionally, human brucellosis has a serious medical impact worldwide, which affects people of all age groups and of both sexes. The eradication poses major difficulties because this disease is largely under diagnosed and underreported. Furthermore, brucellosis in human beings is rarely fatal; it can be severely debilitating and disabling. A renewed scientific interest in human brucellosis has been fuelled by its recent re-emergence and enhanced surveillance in many areas of the world because of regarding the disease as a class B bioterrorist agent and remains the world?s most common bacterial zoonosis. A cross-sectional study was conducted in the period from March, 2013 to March, 2014 to estimate seroprevalence ...
Diagnosis Code A23.0 information, including descriptions, synonyms, code edits, diagnostic related groups, ICD-9 conversion and references to the diseases index.
Brucellosis is primarily a worldwide zoonotic disease caused by Brucella species. The genus Brucella contains highly infectious species that are classified ...
Metode hisapan sering digunakan pada aborsi yang merupakan cara yang ilegal secara medis walaupun dilakukan oleh tenaga medis. Tabung suntik yang besar dilekatkan pada ujung kateter yang dapat dilakukan penghisapan yang berakibat ruptur dari chorionic sac dan mengakibatkan abortus. Cara ini aman asalkan metode aseptic dijalankan, jika penghisapan tidak lengkap dan masih ada sisa dari hasil konsepsi maka dapat mengakibatkan infeksi.. Tujuan dari merobek kantong kehamilan adalah jika kantong kehamilan sudah rusak maka secara otomatis janin akan dikeluarkan oleh kontraksi uterus. Ini juga dapat mengakibatkan dilatasi saluran cerviks, yang dapat mengakhiri kehamilan. Semua alat dapat digunakan dari pembuka operasi sampai jari-jari dari ban sepeda.. Paramedis yang melakukan abortus suka menggunakan kateter yang kaku. Jika digunakan oleh dokter maupun suster, yang melakukan mempunyai pengetahuan anatomi dan menggunakan alat yang steril maka risikonya semakin kecil. Akan tetapi orang awam tidak ...
Metode hisapan sering digunakan pada aborsi yang merupakan cara yang ilegal secara medis walaupun dilakukan oleh tenaga medis. Tabung suntik yang besar dilekatkan pada ujung kateter yang dapat dilakukan penghisapan yang berakibat ruptur dari chorionic sac dan mengakibatkan abortus. Cara ini aman asalkan metode aseptic dijalankan, jika penghisapan tidak lengkap dan masih ada sisa dari hasil konsepsi maka dapat mengakibatkan infeksi.. Tujuan dari merobek kantong kehamilan adalah jika kantong kehamilan sudah rusak maka secara otomatis janin akan dikeluarkan oleh kontraksi uterus. Ini juga dapat mengakibatkan dilatasi saluran cerviks, yang dapat mengakhiri kehamilan. Semua alat dapat digunakan dari pembuka operasi sampai jari-jari dari ban sepeda.. Paramedis yang melakukan abortus suka menggunakan kateter yang kaku. Jika digunakan oleh dokter maupun suster, yang melakukan mempunyai pengetahuan anatomi dan menggunakan alat yang steril maka risikonya semakin kecil. Akan tetapi orang awam tidak ...
Abstract The penultimate step in the biosynthesis of riboflavin (vitamin B2) involves the condensation of 3,4-dihydroxy-2-butanone 4-phosphate with 5-amino-6-ribitylamino-2,4(1H,3H..
Side 1944 - Morphologically, also, melitensis is readily distinguished iiom faecalis by its smaller size and by its great numbers of coccoid cells. There is given herewith a general description of melitensis, the type species of the genus Brucella: Minute rods with many coccoid cells ; (the cells of two-day cultures grown on the surface of plain agar and stained with carbol fuchsin appear about 0.5 of a micron wide and 0.5 to 2 microns long); not forming endospores; non-motile; aerobic, or preferring a slightly... ...
The NYT has posted an article, Abortion qualms on the morning-after pill may be unfounded, claiming they dont cause abortions after all.
En tres municipios de Córdoba (Andalucía, España) se identificaron once casos de brucelosis. Se realizó un estudio de casos y controles, seleccionando tres casos por cada control. Las personas que consumieron queso de cabra fresco sin higienizar elaborado en una granja situada en el territorio epidémico, presentaban un mayor riesgo de padecer brucelosis (OR=21,6, IC 95%=1,6-639,8). Se identificó Brucella melitensis serotipo 3 en muestras clínicas, y en tejido de cabras y muestras de leche de la granja del rebaño. Se adoptaron medidas preventivas y el brote fue contenido tras la retirada del mercado de todos los quesos sospechosos, la desinfección y limpieza adicional de la granja y la realización de actividades de promoción sanitaria.
Description of Brucellosis, what is is, how it is transmitted, which countries have Brucellosis and what can you do to prevent it
Antibiotic activity of molds for Brucella. The isolation of two active substances from a strain of Aspergillus terreus Thom: one in crystalline form, the other as an amorphous concentrate ...
JE Hyung Gon , SONG Hyun Circulation journal : official journal of the Japanese Circulation Society 72(3), 500-501, 2008-02-20 J-STAGE 医中誌Web 参考文献8件 ...
PAULIN, Lília Márcia Silva et al. Fluorescence polarization assay, competitive enzyme-linked immunosorbent assay (ELISA-C) and indirect ELISA for the diagnosis of brucellosis in buffaloes (Bubalus bubalis). Cienc. Rural [online]. 2012, vol.42, n.9, pp.1621-1626. Epub 14-Ago-2012. ISSN 1678-4596. http://dx.doi.org/10.1590/S0103-84782012005000070.. The objective of the present study was to compare the performance of three serological tests for diagnosis of Brucella abortus infections in buffaloes (Bubalus bubalis). Serum samples collected from 696 adult females were submitted to the competitive enzyme-linked immunosorbent assay (ELISA-C), (I-ELISA), fluorescence polarization test (FPA), 2-mercaptoethanol test (2-ME) and complement fixation test (CFT). The gold standard was the combination of CFT and 2-ME, considering as positive the reactors in both CFT and 2-ME, and as negative those non-reactors. ROC analyses were done for C-ELISA, I-ELISA and FPA and the Kappa agreement index were also ...
March 24, 2004. Goat milk sold in the United States may soon be better protected against brucellosis-causing bacteria, thanks to recent research conducted by two U.S. Department of Agriculture agencies in Ames, Iowa. A test for detecting the bacteria Brucella melitensis in bulk goat milk has been developed by research chemist Louisa Tabatabai of the Agricultural Research Services National Animal Disease Center (NADC), Barbara Martin of the Animal and Plant Health Inspection Services (APHIS) National Veterinary Services Laboratories, and graduate student Nathan Funk of Iowa State University. The test relies on an adaptation of an enzyme-linked immunoassay (ELISA) that Tabatabai helped develop in 1984 for testing cattle for B. abortus.. B. melitensis, one of six known species of Brucella bacteria that induce abortions in animals, mainly infects sheep and goats. In humans, B. melitensis infection causes Malta fever, which is characterized by fever and headaches.. Few cases of this infection in ...
Impact Factor: 2,450 2015. Markowicz M, Kivaranovic D, Stanek G. Testing patients with non-specific symptoms for antibodies against Borrelia burgdorferi sensu lato does not provide useful clinical information about their aetiology. Clin Microbiol Infect. 2015 Aug 29. pii: S1198-743X(15)00791-0. doi: 10.1016/j.cmi.2015.08.005. [Epub ahead of print] Markowicz M, Ladstatter S, Schötta AM, Reiter M, Pomberger G, Stanek G. Oligoarthritis caused by Borrelia bavariensis, Austria, 2014. Emerg Infect Dis. 2015 Jun;21(6):1052-4. doi: 10.3201/eid2106.141516. Reiter, M., A. Schötta, A. Müller, H. Stockinger, and G. Stanek. A newly established real-time PCR for detection of Borrelia miyamotoi in Ixodes ricinus ticks. Ticks and Tick-Borne Diseases 6:303 (2015) 2014. Tobudic S., K. Nedomansky, W. Poeppl, M. Müller, A. Faas, G. Mooseder, F. Allerberger, G. Stanek, H. Burgmann. Seroprevalence for Coxiella burnetii, Francisella tularensis, Brucella abortus and Brucella melitensis in Austrian adults: a ...
Aim: We determined the antibody response in cattle naturally infected with brucellosis and normal healthy adult cattle vaccinated during calf hood with strain 19.. Materials and Methods: The antibody titers were measured by standard tube agglutination test (STAT), microtiter plate agglutination test (MAT), indirect hemagglutination assay (IHA), and indirect enzyme-linked immunosorbent assay (iELISA) as per standard protocols.. Results: The mean STAT titers were 1.963 0.345 in infected cattle and 1.200 0.155 in healthy vaccinated cattle. The difference was extremely significant (p,0.0001). The mean MAT titers were 2.244 0.727 in infected cattle and 1.200 0.155 in healthy vaccinated cattle. The difference was very significant (p,0.005). The mean IHA titers in infected cattle were 2.284 0.574, and those in healthy vaccinated cattle were 1.200 0.155. The difference was extremely significant (p=0.0002). However, the difference in mean iELISA titers of infected cattle (1.3678 0.014) and healthy ...
Background: Brucellosis is a zoonotic disease caused by a Gram negative rod named after Bruce in 1887. It is a zoonosis of worldwide distribution. In Saudi Arabia, the prevalence is high and is attributed to widespread animal husbandry and to the traditional drinking of raw milk. Shoulder brucellosis commonly seen in old population. We have recently treated an old patient diagnosed with brucellosis, who was seen with clinical, radioisotopic, microbiologic, and surgical evidence of shoulder septic arthritis, a rare site of Brucella involvement. Case presentation: A 75 year old male presented with history of fever for the past 10 days with associated right shoulder pain. He was admitted under internal medicine for further investigations for fever of unknown origin. Right shoulder pain was described as generalized dull ache of with gradual onset .Patient Denied any contact with any sheep, animal urine or dairy products, there was no obvious source of ongoing infection and no history of recent ...
Brucella abortus *Brucellosis. Bartonellaceae. *Bartonellosis: Bartonella henselae *Cat-scratch disease. *Bartonella quintana * ...
Brucella abortus *Brucellosis. Bartonellaceae. *Bartonellosis: Bartonella henselae *Cat-scratch disease. *Bartonella quintana * ...
Brucella abortus *Brucellosis. Bartonellaceae. *Bartonellosis: Bartonella henselae *Cat-scratch disease. *Bartonella quintana * ...
Brucella abortus *Brucellosis. Bartonellaceae. *Bartonellosis: Bartonella henselae *Cat-scratch disease. *Bartonella quintana * ...
Brucella abortus *Brucellosis. Bartonellaceae. *Bartonellosis: Bartonella henselae *Cat-scratch disease. *Bartonella quintana * ...
Brucella abortus *Brucellosis. Bartonellaceae. *Bartonellosis: Bartonella henselae *Cat-scratch disease. *Bartonella quintana * ...
Brucella abortus *Brucellosis. Bartonellaceae. *Bartonellosis: Bartonella henselae *Cat-scratch disease. *Bartonella quintana * ...
Brucella abortus *Brucellosis. Bartonellaceae. *Bartonellosis: Bartonella henselae *Cat-scratch disease. *Bartonella quintana * ...
Brucella abortus *Brucellosis. Bartonellaceae. *Bartonellosis: Bartonella henselae *Cat-scratch disease. *Bartonella quintana * ...
MARR AG, WILSON PW (1954). "The alanine racemase of Brucella abortus". Arch. Biochem. Biophys. 49 (2): 424-33. doi:10.1016/0003 ...
ALTENBERN RA, HOUSEWRIGHT RD (1953). "Transaminases in smooth Brucella abortus, strain 19" (PDF). J. Biol. Chem. 204 (1): 159- ...
Detection of Antibody to Brucella abortus". Methods. 22: 71-76 - via Elsevier Science Direct. Jameson, David (2003). " ...
Gor D, Mayfield JE (1992). "Cloning and nucleotide sequence of the Brucella abortus groE operon". Biochim. Biophys. Acta. 1130 ...
Ugalde, J.E.; Comerci, D.J.; Leguizamon, M.S.; Ugalde, R.A. (2003). "Evaluation of Brucella abortus phosphoglucomutase (pgm) ... Ugalde, J.E.; Czibener, C.; Feldman, M.F.; Ugalde, R.A. (2000). "Identification and characterization of the Brucella abortus ... Pei, J.; Ficht, T.A. (2004). "Brucella abortus rough mutants are cytopathic for macrophages in culture". Infection and Immunity ... "Establishment of systemic Brucella melitensis infection through the digestive tract requires urease, the type IV secretion ...
ISBN 0-8018-5789-9. Groocock, C.M.; Staak, C. (1969). "The isolation of Brucella abortus from a waterbuck (Kobus ellipsiprymnus ...
Currently, all cattle of ages 3-8 months is required to be given the Brucella abortus strain 19 vaccine. Australia is free of ... The first state-federal cooperative efforts towards eradication of brucellosis caused by Brucella abortus in the U.S. began in ... Brucella species survive well in aerosols and resist drying. Brucella and all other remaining biological weapons in the U.S. ... Four species infect humans: B. abortus, B. canis, B. melitensis, and B. suis. B. abortus is less virulent than B. melitensis ...
On the other hand, ALOX5 gene knockout mice demonstrate an enhanced resistance and lessened pathology to Brucella abortus ... "5-Lipoxygenase negatively regulates Th1 response during Brucella abortus infection in mice". Infection and Immunity. 83 (3): ...
Danish veterinarian Bernhard Bang isolates Brucella abortus as the agent of Brucellosis. Epinephrine discovered by John Jacob ...
GINOZA HS, ALTENBERN RA (1955). "The pantothenate-synthesizing enzyme in cell-free extracts of Brucella abortus, strain 19". ...
Aside from these bacteria, PPIB has been identified in Brucella abortus, Mycobacterium tuberculosis, Bacillus subtilis and ... "Intracellularly Induced Cyclophilins Play an Important Role in Stress Adaptation and Virulence of Brucella abortus". Infection ...
He was chairman of the committee on Brucella abortus infection and on Johne's disease (Paratuberculosis). He also chaired the ...
Beh KJ (1976). "Immunoglobulin class specificity of non-agglutinating antibody produced in cattle following Brucella abortus 45 ...
A functional water channel protein in the pathogenic bacterium Brucella abortus. Microbiol 146(12):3251-3257. doi: 3251-3257. ... seperti Brucella abortus yang menyebabkan brucellosis pada sapi dan Bacillus anthracis yang menyebabkan antraks.[60] Untuk ...
"Molecular epidemiology of Brucella abortus isolates from cattle, elk, and bison in the United States, 1998 to 2011". Appl. ...
Brucella abortus (Q57B94), Brucella suis (Q8FYS0) and Brucella melitensis (Q8YJ29). The biochemical mechanism of proline ...
Some bacteria like Brucella abortus, Moraxella catarrhalis and Streptococcus pneumoniae have developed resistance towards ...
Brucella spp. cattle, goats infected milk or meat Bubonic plague, Pneumonic plague, Septicemic plague, Sylvatic plague Yersinia ... Chlamydophila abortus domestic livestock, particularly sheep close contact with postpartum ewes COVID-19 severe acute ...
Antitumor process using a Brucella abortus preparation, retrieved 2016-01-26 Selected Patents Dowling, Patricia W.; Youngner, ... antitumor processes of Brucella, and numerous contributions to cold adapted influenza virus. Youngner was self-effacing. He ...
... sequencing to rapidly and comprehensively determine the complete genome sequence of the attenuated Brucella abortus vaccine ... abortus, 9-941 and 2308. The two S19 chromosomes are 2,122,487 and 1,161,449 bp in length. A total of 3062 genes were ... The Brucella abortus strain S19, a spontaneously attenuated strain, has been used as a vaccine strain in vaccination of cattle ...
Brucella abortus is a facultative intracellular bacterial pathogen, which causes abortion in cattle and undulant fever in human ... B. abortus strain RB51 (Strain RB51) is the official vaccine for bovine brucellosis in the USA. B. abortus strain RB51 can be ... abortus strain RB51, outer-membrane (OM), periplasmic space (PS) and in the cytoplasmic region (CR) of B. abortus strain RB51. ... in which the 18 kDa Brucella lipoprotein and the Brucella Cu/Zn SOD protein signal sequences were added to the GFP sequence to ...
The prokaryotic B. abortus, is spore-forming, nonmotile and aerobic. Brucella abortus enters phagocytes that invade human and ... Brucella abortus is a Gram-negative proteobacterium in the family Brucellaceae, and is one of the causative agents of ... Dorneles, EM; Sriranganathan, N; Lage, AP (8 July 2015). "Recent advances in Brucella abortus vaccines". Veterinary research. ... B. abortus causes abortion and infertility in adult cattle, and is a zoonosis which is present worldwide. Humans are commonly ...
... abortus,biological,advanced biology technology,biology laboratory technology,biology device technology,latest biology ... Multiporator / Electropo... Making electrocompetent cells: ... Electroporation of cells: Grow ...,Brucella, ... 4308 915.508 12/2001 Microorganism Brucella abortus Cell type Bacteria, gram negative Molecules injected Plasmid DNA (pBA.sodkn ...
... On May 26-27, 1997, nine persons (a farmer, four veterinary ... Experimental infection of pregnant cattle with the vaccine candidate Brucella abortus strain RB51: pathologic, bacteriologic, ... and a necropsy on a stillborn calf that died because of Brucella abortus infection. The infection was confirmed by isolation of ... vaccinated with Brucella abortus strain RB51. J Vet Diagn Invest 1997;9:363-7. ...
An Unusual Case of Brucella abortus Prosthetic Joint Infection.. Walsh J1, Gilleece A2, Fenelon L1, Cogley D3, Schaffer K1. ... Brucellosis is a systemic infection caused by brucella species. Prosthetic joint infection due to brucella species is rare. We ...
Brucella abortus strain BDW chromosome 2, complete sequence Brucella abortus strain BDW chromosome 2, complete sequence. gi, ...
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Methane metabolism - Brucella abortus 2308 [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show description , ...
M00130 Inositol phosphate metabolism, PI=> PIP2 => Ins(1,4,5)P3 => Ins(1,3,4,5)P4 ...
Distribution of Radioiodinated, Acetone-killed Brucella abortus in the Tissues of Normal and Immune Mice. *D. SULITZEANU1. ... SULITZEANU, D. Distribution of Radioiodinated, Acetone-killed Brucella abortus in the Tissues of Normal and Immune Mice. Nature ... Induced Resistance of Mice to Infection with Brucella abortus 2308 through Vaccination with BCG *A. BEKIERKUNST ...
This work demonstrates that Brucella lipopolysaccharide (LPS) preparations are a family of related molecules which display ... Heterogeneity of Brucella Abortus Lipopolysaccharides Res Microbiol. 1995 Sep;146(7):569-78. doi: 10.1016/0923-2508(96)80563-8 ... Smooth-type LPS (S-LPS) from B. abortus demonstrated three broad high-molecular-weight bands corresponding to S-LPS, and a low- ... This work demonstrates that Brucella lipopolysaccharide (LPS) preparations are a family of related molecules which display ...
This assay utilizes antigen from Brucella abortus strain W99. ... for the measurement of human IgA antibodies to Brucella abortus ... The Brucella abortus IgA ELISA Kit is intended ... Brucella abortus IgA ELISA Kit (CE). Packaging size: 96 Wells ... The Brucella abortus ELISA Kit is intended for the measurement of human IgG, IgA, or IgM antibodies to Brucella abortus. This ... The Brucella abortus IgA ELISA Kit is intended for the measurement of human IgA antibodies to Brucella abortus. This assay ...
... MMWR 47(09);172-175 Publication date: 03/13/1998. Table of ... Experimental infection of pregnant cattle with the vaccine candidate Brucella abortus strain RB51: pathologic, bacteriologic, ... and a necropsy on a stillborn calf that died because of Brucella abortus infection. The infection was confirmed by isolation of ... a stable rough strain of Brucella abortus. Vet Microbiol 1991;28:171-88. * Cheville NF, Jensen AE, Halling SM, et al. Bacterial ...
cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › BrucellaBrucella abortus ... sp,Q2YK69,NANM_BRUA2 N-acetylneuraminate epimerase OS=Brucella abortus (strain 2308) OX=359391 GN=nanM PE=3 SV=1 ... Brucella abortus (strain 2308). ,p>This subsection of the ,a href="http://www.uniprot.org/help/names_and_taxonomy_section"> ...
Brucella abortus strain 2308. Brucella abortus (strain 2308): entries and gene names ... cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › BrucellaBrucella abortus ... sp,P0C0Z6,RS15_BRUA2 30S ribosomal protein S15 OS=Brucella abortus (strain 2308) OX=359391 GN=rpsO PE=3 SV=1 ... Brucella abortus (strain 2308). ,p>This subsection of the ,a href="http://www.uniprot.org/help/names_and_taxonomy_section"> ...
... foi empregada na detecção de Brucella spp. em 300 amostras de produtos... ... utilizando-se primers desenhados a partir do gene que codifica uma proteína imunogênica de 31 KDa de Brucella abortus, ... Todas as amostras positivas na PCR para Brucella spp. foram confirmadas como sendo da espécie Brucella abortus pela técnica de ... All positive samples for Brucella spp. were confirmed as Brucella abortus by PCR using species-specific primers. Hemi-nested ...
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The two-component system BvrR/BvrS essential for Brucella abortus virulence regulates the expression of outer membrane proteins ... The two-component system BvrR/BvrS essential for Brucella abortus virulence regulates the expression of outer membrane proteins ... The two-component system BvrR/BvrS essential for Brucella abortus virulence regulates the expression of outer membrane proteins ... The two-component system BvrR/BvrS essential for Brucella abortus virulence regulates the expression of outer membrane proteins ...
Comparative distribution of Brucella abortus, Brucella melitensis and Brucella ovis in experimentally infected pregnant sheep. ... Brucella abortus strain RB51 used for vaccination was selected by growth of B. abortus strain 2308 in the presence of ... Brucella abortus in the bitch. Veterinary Record, 99:85-86.. Britov VA, Melnikova AI et al., 1979. Transmission of Brucella ... Brucella abortus is to a certain extent distinguishable from other Brucellae by biochemical reactions and by serological means ...
"Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR," ... Trueperella pyogenes and Brucella abortus Coinfection in a Dog and a Cat on a Dairy Farm in Egypt with Recurrent Cases of ... Trueperella pyogenes was isolated from a dog and a cat with a mixed infection with Brucella abortus. Both lived on a dairy ... Both animals lived on a dairy farm with recurrent cases of abortion and mastitis due to Brucella (B.) abortus biovar (bv) 1 ...
Brucella Abortus Lipopolysaccharide Is Mitogenic for Spleen Cells of Endotoxin-Resistant C3H/HeJ Mice. Edgardo Moreno and David ... Brucella Abortus Lipopolysaccharide Is Mitogenic for Spleen Cells of Endotoxin-Resistant C3H/HeJ Mice ... Preparations of lipopolysaccharide (LPS) from rough and smooth strains of Brucella abortus were mitogenic for spleen cells of ... Brucella Abortus Lipopolysaccharide Is Mitogenic for Spleen Cells of Endotoxin-Resistant C3H/HeJ Mice ...
B. abortus infection induces apoptosis and inhibits osteoblast function. DHEA treatment reversed the effect of B. abortus ... B. abortus infection induces apoptosis and inhibits osteoblast function. DHEA treatment reversed the effect of B. abortus ... abortus on osteoblast differentiation and function. In contrast, cortisol increased the effect of B. abortus infection. ... abortus on osteoblast differentiation and function. In contrast, cortisol increased the effect of B. abortus infection. ...
By confocal microscopy, we demonstrated that TLR7 is colocalized with B. abortus in LAMP-1+ Brucella-containing vacuoles. ... By confocal microscopy, we demonstrated that TLR7 is colocalized with B. abortus in LAMP-1+ Brucella-containing vacuoles. ... Our results suggest that TLR3 and TLR7 are not required to control Brucella infection in vivo but they play an important role ... Our results suggest that TLR3 and TLR7 are not required to control Brucella infection in vivo but they play an important role ...
Brucella abortus is a Gram-negative bacterium causing brucellosis. Although B. abortus is known to infect via the oral route, ... To assess the blocking effect of anti-Hsp60 Ab on B. abortus uptake by M cells, 1 million GFP-B. abortus (17), B. abortus 544, ... Cutting Edge: Brucella abortus Exploits a Cellular Prion Protein on Intestinal M Cells as an Invasive Receptor. Gaku Nakato, ... Essential role of the VirB machinery in the maturation of the Brucella abortus-containing vacuole. Cell. Microbiol. 3: 159-168. ...
  • Smooth-type LPS (S-LPS) from B. abortus demonstrated three broad high-molecular-weight bands corresponding to S-LPS, and a low-molecular-weight band corresponding to O antigen lacking rough-type LPS (R-LPS). (nih.gov)
  • This assay utilizes antigen from Brucella abortus strain W99. (mpbio.com)
  • In an effort to explore the role of O antigen in the interaction between Brucella abortus and macrophages, we have monitored the uptake of rough mutants and survival in vitro by using the murine macrophage cell line J774.A1. (asm.org)
  • Among these, the O antigen has been classified as a major virulence determinant of Brucella and is essential for survival of the classical species in the host. (asm.org)
  • Recently, evidence has been presented that suggests that O antigen is critical in inhibiting programmed cell death (apoptosis) and that a rough derivative of Brucella melitensis defective in the expression of glycosyl transferase ( wboA ) fails to block apoptosis, resulting in death of the cells along with any internalized bacteria ( 17 ). (asm.org)
  • The Brucella -specific production of gamma interferon (IFN-γ) in blood plasma produced by lymphocytes in antigen-stimulated whole-blood samples may be detected by indirect enzyme-linked immunosorbent assay (iELISA) ( 8 , 9 ). (asm.org)
  • Induction Murine Models of Chronic Fatigue Syndrome by Brucella abortus Antigen Injections: Is Anemia Induced or Not? (semanticscholar.org)
  • With the National Standard serum the titres to B. melitensis were consistently lower than those to B. abortus antigen. (up.ac.za)
  • Although this may be due to the vagaries of the test it also warrants closer investigation of the animals concerned to see whether M-antigen predominant Brucella biotypes are possibly present. (up.ac.za)
  • The use of the dual antigens could identify herds which are infected only with A-antigen predominant brucellae but would not be reliable for classifying individual animals. (up.ac.za)
  • Among Brucella immunogenes, antigen based on ribosomal preparation has been widely investigated. (ac.ir)
  • Since the protective antigen (PA) of B. anthracis is known to induce protective antibodies, it was decided that the objective of this research was to test whether the gene encoding PA could be expressed in Brucella producing a bivalent vaccine to protect against both brucellosis and anthrax. (vt.edu)
  • These results suggested that B. abortus EryC would be a potential reagent for diagnosis for bovine brucellosis as a single protein antigen. (mendeley.com)
  • Brucella antigen in liver and spleen from Asian badgers infected with Brucella melitensis , northwestern China. (cdc.gov)
  • The aim of the present study was to set the conditions for the use of a commercially available antigen (the O-polysaccharide from B. abortus 1119-3 conjugated with fluorescein isothiocyanate) for the FPA assay in a 96-well microplate format, and to compare its diagnostic performance with the conventional agglutination tests currently used in Argentina. (scitechnol.com)
  • Antibody response and antigen-specific gamma-interferon profiles of vaccinated and unvaccinated pregnant sheep experimentally infected with Brucella melitensis . (springer.com)
  • The integrity of product was confirmed by Western-blot analysis using a standard rabbit anti Brucella abortus ribosomal protein L7/L12 antibody. (ac.ir)
  • Background: Lipopolysaccharides (LPSs) and several antigenic proteins of Brucella have been considered for preparation of diagnostic reagents and subunit vaccines. (ac.ir)
  • Furthermore several proteins of Brucella had similar reactions against all sera. (ac.ir)
  • A large number of hypothetical genes potentially encoding small proteins of unknown function are annotated in the Brucella abortus genome. (elsevier.com)
  • The proteins unique to biofilms and planktonic B. abortus were separated by two‑dimensional (2‑D) electrophoresis and then identified by matrix‑assisted laser desorption/ionization‑tandem time of flight‑mass spectrometry (MALDI‑TOF/TOF‑MS). High‑throughput sequencing and bioinformatic analyses were performed to identify differentially expressed genes between B. abortus cultured under biofilm and planktonic conditions. (spandidos-publications.com)
  • Here we report that while screening Brucella ORFeome for polar proteins in Brucella abortus , AidB was found to localize at the new pole, as well as at the constriction site in dividing cells. (biomedcentral.com)
  • Other B. abortus proteins, Omp16, PP26, and CP39 were also purified and their seroreactivities were compared. (mendeley.com)
  • B. abortus expresses stress response proteins under conditions observed within the host macrophage ( 38 ), including low pH and reactive oxidative agents (O 2 · − and H 2 O 2 ). (asm.org)
  • A técnica de PCR, utilizando-se "primers" desenhados a partir do gene que codifica uma proteína imunogênica de 31 KDa de Brucella abortus , foi empregada na detecção de Brucella spp. (usp.br)
  • A PCR assay using primers designed based on the gene that encodes a 31 KDa immunogenic protein from Brucella abortus was used in the detection of Brucella spp. (usp.br)
  • Deletion of the BCSP31 gene of Brucella abortus by replacement. (asm.org)
  • The 31-kDa salt-extractable immunogenic protein, BCSP31, was deleted from several Brucella abortus strains by replacement with a marker gene encoding resistance to the antibiotics kanamycin and neomycin. (asm.org)
  • The BCSP31 gene replacement plasmids, constructed with ColE1-derived vectors, were introduced by electroporation into B. abortus strain 19 (S19), into a rough variant of B. abortus S19, and into B. abortus S2308, and antibiotic-resistant transformants were isolated. (asm.org)
  • In this study, the immunogenic ribosomal protein L7/L12 gene from Brucella abortus, S19, was amplified by PCR and sub-cloned to prokaryotic expression vector pET28a. (ac.ir)
  • and ( i ) an IS 711 element downstream of the bp26 gene in Brucella spp. (aaccjnls.org)
  • In this study, we constructed the Brucella abortus ( B. abortus ) S2308 mutant strain Δ22915, in which the putative lytic transglycosylase gene BAB_RS22915 was deleted. (biomedcentral.com)
  • The present study aimed to explore the differences in protein and gene expression of Brucella abortus cultured under biofilm and planktonic conditions. (spandidos-publications.com)
  • Moreover, a Brucella melitensis mutant in the alkA gene was found to be attenuated in mice (Pascal Lestrate, Ph.D. thesis, 2003). (biomedcentral.com)
  • In the present study, the eryC gene encoding B. abortus D-erythrulose 1-phosphate dehydrogenase, which is involved in the erythritol metabolism in virulent B. abort us strain but is absent from a B. abortus vaccine strain (S19), was cloned. (mendeley.com)
  • The Mn-responsive repressor Mur downregulates the expression of mntH, the gene encoding the sole high affinity Mn importer in Brucella in response to increased intracellular levels of Mn. (ecu.edu)
  • We report that the Brucella O-PS can be structurally and antigenically modified using wbdR, the acetyl-transferase gene involved in N-acetyl-perosamine synthesis in Escherichia coli O157:H7. (figshare.com)
  • Moreover, deletion of the Brucella formyltransferase gene wbkC in wbdR constructs generated bacteria producing only N-acetyl-perosamine homopolymers, proving that wbdR can replace for wbkC. (figshare.com)
  • Vector sequences were either absent or linked to the genome, indicating that ColE1-derived plasmids are not maintained in B. abortus. (asm.org)
  • BLACKSBURG, Va., June 4, 2008 - Researchers at the Virginia Bioinformatics Institute at Virginia Tech and the National Animal Disease Center in Ames, Iowa, and collaborators at 454 Life Sciences have sequenced the genome of Brucella abortus strain S19. (vt.edu)
  • In Escherichia coli , AidB protein takes part of the adaptative response to alkylating agents that could damage the genome [ 11 ], suggesting that AidB homolog could play a similar role in B. abortus . (biomedcentral.com)
  • Such analyses are subject to variable interpretations and must be performed by skilled technicians who are at high risk because most clinical Brucella strains are highly pathogenic. (aaccjnls.org)
  • As with all pathogenic bacteria, the Brucella spp. (ecu.edu)
  • During this process, colocalization of cellular prion protein (PrP C ) and B. abortus was evident on the apical surface as well as in subapical vacuolar structures in M cells. (jimmunol.org)
  • Abtahi H, Salmanian A H, Rafati S, Behzadian Nejad G, Mohammad Hassan Z. High Level Expression of Recombinant Ribosomal Protein (L7/L12) from Brucella abortus and Its Reaction with Infected Human Sera. (ac.ir)
  • Biochemical analyses demonstrated that a purified recombinant B. abortus PutA protein possesses quintessential proline dehydrogenase activity, as PutA is capable of catalysing the conversion of proline to glutamate. (microbiologyresearch.org)
  • Although B. abortus-infected glial cells secreted IL-1b and TNF-α, activation of HBMEC was dependent on IL-1b because CS from B. abortus-infected astrocytes and microglia deficient in caspase-1 and apoptosis-associated speck-like protein containing a CARD failed to induce HBMEC activation. (elsevier.com)
  • The small protein CydX is required for function of cytochrome bd oxidase in Brucella abortus. (elsevier.com)
  • The Brucella ORFeome, a collection of cloned predicted coding sequences, was placed in fusion with yellow fluorescent protein (YFP) coding sequence and screened for polar localizations in B. abortus . (biomedcentral.com)
  • We hypothesize that translocation of the T4SS substrate VceC activates the unfolded protein response (UPR) with consequent induction of NF-?B-dependent inflammatory responses, thereby contributing to B. abortus- induced abortion. (grantome.com)
  • A total of 66 isolates of Brucella were recovered from sheep and yaks in the Inner Mongolia, Xinjiang, Qinghai and Gansu provinces of northwest China in 2015 and 2016. (readbyqxmd.com)
  • MLVA identified 98 unique B. abortus genotypes from disclosing isolates in the 387 herds involved in the epidemic. (cdc.gov)
  • Our results show circulation of five B. abortus lineages (I to V) in CR, phylogenetically related to isolates from the United States, United Kingdom, and South America. (cdc.gov)
  • Brucella abortus enters phagocytes that invade human and animal defenses which in turn, cause chronic disease in the host. (wikipedia.org)
  • Exposure to the oxidative burst of host phagocytes is one of the environmental stresses the brucellae must deal with during their intracellular residence. (ecu.edu)
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  • In this paper, we present a review of the main aspects of the vaccines that have been used in the brucellosis control over the years and the current research advances in the development of new B. abortus vaccines. (beds.ac.uk)
  • These results open the possibilities of developing brucellosis vaccines that are both antigenically tagged and lack the diagnostic epitopes of virulent field strains, thereby solving the diagnostic interference created by current vaccines against Brucella. (figshare.com)
  • A specific additional 1320-bp fragment was amplified, and the 450-bp fragment was absent in Brucella strains isolated from marine mammals. (aaccjnls.org)
  • This work demonstrates that Brucella lipopolysaccharide (LPS) preparations are a family of related molecules which display heterogeneity not only at the level of the O polysaccharide, but also at the core oligosaccharide and the lipid A. Sodium dodecyl sulphate/polyacrylamide gel electrophoresis and Western blotting showed that LPS from Brucella strains displayed molecular weight and antigenic heterogeneity. (nih.gov)
  • B. abortus R-LPS displayed four bands in increasing proportions as the molecular weight diminished. (nih.gov)
  • Because these properties relate to the structure of the Brucella outer membrane ( 3-5 ), we reasoned that some of its molecular features should be under the control of BvrR/BvrS. (pnas.org)
  • Molecular Epidemiology of Brucella abortus in Northern Ireland-1991 to 2012. (cdc.gov)
  • Multi-locus variable number tandem repeat VNTR analysis (MLVA) was used to investigate the molecular epidemiology of a well-characterised Brucella abortus epidemic in Northern Ireland involving 387 herds between 1991 and 2012. (cdc.gov)
  • Scant information exists about the molecular mechanisms employed by Brucella abortus to combat host defenses or to persist and replicate within host cells. (asm.org)
  • This study aimed at molecular detection and characterization of Brucella spp from Pakistan. (thefreelibrary.com)
  • The brucellae are facultative intracellular parasites of animals and humans causing a disease of worldwide importance. (pnas.org)
  • B. abortus is a significant zoonotic risk and animal health problem in this production system, therefore further studies on humans is recommended. (biomedcentral.com)
  • Brucella is able to pass from animals to humans with relative ease and poses a significant public health burden for workers in the livestock industry. (vt.edu)
  • Detection of IgM and IgG antibodies against Brucella by dot-ELISA in humans. (elisakits.co.uk)
  • B. abortus primarily targets organs of the reticuloendothelial system and reproductive system in primary hosts ( 14 , 40 ), while diverse manifestations of brucellosis are observed in humans, a secondary host, and include fever, anorexia, and malaise. (asm.org)
  • We homogenized spleen samples of badgers and the 14 aborted sheep fetuses and inoculated these homogenates onto individual Brucella agar plates, which we then incubated at 37°C in an atmosphere of 5% CO 2 for 5 days. (cdc.gov)
  • In agriculturally important ruminants, the brucellae invade the trophoblastic epithelium of the gravid uterus ( 5 ), where their rapid multiplication leads to abortion and infertility ( 50 ). (asm.org)
  • Similar to other facultative intracellular parasites, Brucella organisms survive outside cells, but they must infect and replicate intracellularly in animals to perpetuate. (pnas.org)
  • Although B. abortus is known to infect via the oral route, the entry site in the gastrointestinal tract has been unclear. (jimmunol.org)
  • The genomes of two strains of B. abortus have been completely sequenced and these bacteria are somewhat unusual in having two separate chromosomes. (blogspot.com)
  • Pathological, immunohistochemical and bacteriological study of tissues and milk of cows and fetuses experimentally infected with Brucella abortus. (semanticscholar.org)
  • Culture supernatants (CS) from glial cells (astrocytes and microglia) infected with B. abortus also induced activation of HBMEC, but to a greater extent. (elsevier.com)
  • Brucella abortus confirmed herd incidence in Northern Ireland 1991-2012. (cdc.gov)
  • Absorption experiments with immune sera revealed that the wbdR constructs triggered antibodies to new immunogenic epitope(s) and the use of monoclonal antibodies proved that B. abortus and B. melitensis wbdR constructs respectively lacked the A or M epitopes, and the absence of the C epitope in both backgrounds. (figshare.com)
  • Brucellae are intracellular pathogens that have the ability to survive and multiply inside professional phagocytic cells. (biomedcentral.com)
  • Wild-type B. abortus invasion efficiency was greater in phagocytic cells than in epithelial cells. (bvsalud.org)
  • The results also indicated that there are different factors involved in the invasion of B. abortus into phagocytic cells. (bvsalud.org)
  • Some brucellae may be found in autophagosomes in professional phagocytic cells, but the majority of bacteria remain in phagosomes and strongly affect the phagosome maturation process. (asm.org)