A genus of gram-negative, aerobic bacteria that causes BRUCELLOSIS. Its cells are nonmotile coccobacilli and are animal parasites and pathogens. The bacterium is transmissible to humans through contact with infected dairy products or tissue.
A species of the genus BRUCELLA whose natural hosts are cattle and other bovidae. Abortion and placentitis are frequently produced in the pregnant animal. Other mammals, including humans, may be infected.
A species of the genus BRUCELLA whose natural hosts are sheep and goats. Other mammals, including humans, may be infected. In general, these organisms tend to be more virulent for laboratory animals than BRUCELLA ABORTUS and may cause fatal infections.
Infection caused by bacteria of the genus BRUCELLA mainly involving the MONONUCLEAR PHAGOCYTE SYSTEM. This condition is characterized by fever, weakness, malaise, and weight loss.
A bacterial vaccine for the prevention of brucellosis in man and animal. Brucella abortus vaccine is used for the immunization of cattle, sheep, and goats.
A species of the genus BRUCELLA which are pathogenic to SHEEP.
A species of gram-negative bacteria infecting DOGS, the natural hosts, and causing canine BRUCELLOSIS. It can also cause a mild infection in humans.
A disease of cattle caused by bacteria of the genus BRUCELLA leading to abortion in late pregnancy. BRUCELLA ABORTUS is the primary infective agent.
The suborder of aquatic CARNIVORA comprising the WALRUSES; FUR SEALS; SEA LIONS; and EARLESS SEALS. They have fusiform bodies with very short tails and are found on all sea coasts. The offspring are born on land.
A species of gram-negative, obligately aerobic rods. Motility occurs by peritrichous flagella. (From Bergey's Manual of Determinative Bacteriology, 9th ed)
An order of wholly aquatic MAMMALS occurring in all the OCEANS and adjoining seas of the world, as well as in certain river systems. They feed generally on FISHES, cephalopods, and crustaceans. Most are gregarious and most have a relatively long period of parental care and maturation. Included are DOLPHINS; PORPOISES; and WHALES. (From Walker's Mammals of the World, 5th ed, pp969-70)
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (From Stedman, 26th ed)
Diseases of domestic and mountain sheep of the genus Ovis.
Substances elaborated by bacteria that have antigenic activity.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
Inflammation of the EPIDIDYMIS. Its clinical features include enlarged epididymis, a swollen SCROTUM; PAIN; PYURIA; and FEVER. It is usually related to infections in the URINARY TRACT, which likely spread to the EPIDIDYMIS through either the VAS DEFERENS or the lymphatics of the SPERMATIC CORD.
Premature expulsion of the FETUS in animals.
Proteins found in any species of bacterium.
A bright bluish pink compound that has been used as a dye, biological stain, and diagnostic aid.
The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.
A four-carbon sugar that is found in algae, fungi, and lichens. It is twice as sweet as sucrose and can be used as a coronary vasodilator.
Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
Any of numerous agile, hollow-horned RUMINANTS of the genus Capra, in the family Bovidae, closely related to the SHEEP.
Proteins isolated from the outer membrane of Gram-negative bacteria.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
An encapsulated lymphatic organ through which venous blood filters.
Those components of an organism that determine its capacity to cause disease but are not required for its viability per se. Two classes have been characterized: TOXINS, BIOLOGICAL and surface adhesion molecules that effect the ability of the microorganism to invade and colonize a host. (From Davis et al., Microbiology, 4th ed. p486)
Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed)
Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping.
The genetic complement of a BACTERIA as represented in its DNA.
A genus of the family Bovidae having two species: B. bison and B. bonasus. This concept is differentiated from BUFFALOES, which refers to Bubalus arnee and Syncerus caffer.
Diseases of the domestic or wild goat of the genus Capra.
Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A test to detect non-agglutinating ANTIBODIES against ERYTHROCYTES by use of anti-antibodies (the Coombs' reagent.) The direct test is applied to freshly drawn blood to detect antibody bound to circulating red cells. The indirect test is applied to serum to detect the presence of antibodies that can bind to red blood cells.
Tandem arrays of moderately repetitive, short (10-60 bases) DNA sequences which are found dispersed throughout the GENOME, at the ends of chromosomes (TELOMERES), and clustered near telomeres. Their degree of repetition is two to several hundred at each locus. Loci number in the thousands but each locus shows a distinctive repeat unit.
Suspensions of attenuated or killed bacteria administered for the prevention or treatment of infectious bacterial disease.
Mammals of the family Phocoenidae comprising four genera found in the North Pacific Ocean and both sides of the North Atlantic Ocean and in various other seas. They differ from DOLPHINS in that porpoises have a blunt snout and a rather stocky body while dolphins have a beak-like snout and a slender, streamlined body. They usually travel in small groups. (From Walker's Mammals of the World, 5th ed, pp1003-4)
The functional hereditary units of BACTERIA.
Distention of KIDNEY with the presence of PUS and suppurative destruction of the renal parenchyma. It is often associated with renal obstruction and can lead to total or nearly total loss of renal function.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
The TARSAL BONES; METATARSAL BONES; and PHALANGES OF TOES. The tarsal bones consists of seven bones: CALCANEUS; TALUS; cuboid; navicular; internal; middle; and external cuneiform bones. The five metatarsal bones are numbered one through five, running medial to lateral. There are 14 phalanges in each foot, the great toe has two while the other toes have three each.
A rare form of non-Langerhans-cell histiocytosis (HISTIOCYTOSIS, NON-LANGERHANS-CELL) with onset in middle age. The systemic disease is characterized by infiltration of lipid-laden macrophages, multinucleated giant cells, an inflammatory infiltrate of lymphocytes and histiocytes in the bone marrow, and a generalized sclerosis of the long bones.
Inflammatory processes of the muscular walls of the heart (MYOCARDIUM) which result in injury to the cardiac muscle cells (MYOCYTES, CARDIAC). Manifestations range from subclinical to sudden death (DEATH, SUDDEN). Myocarditis in association with cardiac dysfunction is classified as inflammatory CARDIOMYOPATHY usually caused by INFECTION, autoimmune diseases, or responses to toxic substances. Myocarditis is also a common cause of DILATED CARDIOMYOPATHY and other cardiomyopathies.
An independent state consisting of three islands in the Mediterranean Sea, south of Sicily. Its capital is Valetta. The major island is Malta, the two smaller islands are Comino and Gozo. It was a Phoenician and Carthaginian colony, captured by the Romans in 218 B.C. It was overrun by Saracens in 870, taken by the Normans in 1090, and subsequently held by the French and later the British who allotted them a dominion government in 1921. It became a crown colony in 1933, achieving independence in 1964. The name possibly comes from a pre-Indoeuropean root mel, high, referring to its rocks, but a more picturesque origin derives the name from the Greek melitta or melissa, honey, with reference to its early fame for its honey production. (From Webster's New Geographical Dictionary, 1988, p719 & Room, Brewer's Dictionary of Names, 1992, p330)
A cabinet department in the Executive Branch of the United States Government concerned with improving and maintaining farm income and developing and expanding markets for agricultural products. Through inspection and grading services it safeguards and insures standards of quality in food supply and production.
Infections resulting from the implantation of prosthetic devices. The infections may be acquired from intraoperative contamination (early) or hematogenously acquired from other sites (late).
Prostheses used to partially or totally replace a human or animal joint. (from UMDNS, 1999)
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
A publication issued at stated, more or less regular, intervals.

Genomic fingerprinting and development of a dendrogram for Brucella spp. isolated from seals, porpoises, and dolphins. (1/532)

Genomic DNA from reference strains and biovars of the genus Brucella was analyzed using pulsed-field gel electrophoresis (PFGE). Fingerprints were compared to estimate genetic relatedness among the strains and to obtain information on evolutionary relationships. Electrophoresis of DNA digested with the restriction endonuclease XbaI produced fragment profiles for the reference type strains that distinguished these strains to the level of species. Included in this study were strains isolated from marine mammals. The PFGE profiles from these strains were compared with those obtained from the reference strains and biovars. Isolates from dolphins had similar profiles that were distinct from profiles of Brucella isolates from seals and porpoises. Distance matrix analyses were used to produce a dendrogram. Biovars of B. abortus were clustered together in the dendrogram; similar clusters were shown for biovars of B. melitensis and for biovars of B. suis. Brucella ovis, B. canis, and B. neotomae differed from each other and from B. abortus, B. melitensis, and B. suis. The relationship between B. abortus strain RB51 and other Brucella biovars was compared because this strain has replaced B. abortus strain 19 for use as a live vaccine in cattle and possibly in bison and elk. These results support the current taxonomy of Brucella species and the designation of an additional genomic group(s) of Brucella. The PFGE analysis in conjunction with distance matrix analysis was a useful tool for calculating genetic relatedness among the Brucella species.  (+info)

Brucella outer membrane lipoproteins share antigenic determinants with bacteria of the family Rhizobiaceae. (2/532)

Brucellae have been reported to be phylogenetically related to bacteria of the family Rhizobiaceae. In the present study, we used a panel of monoclonal antibodies (MAbs) to Brucella outer membrane proteins (OMPs) to determine the presence of common OMP epitopes in some representative bacteria of this family, i.e., Ochrobactrum anthropi, Phyllobacterium rubiacearum, Rhizobium leguminosarum, and Agrobacterium tumefaciens, and also in bacteria reported to serologically cross-react with brucella, i.e., Yersinia enterocolitica O:9, Escherichia coli O:157, and Salmonella urbana. In particular, most MAbs to the Brucella outer membrane lipoproteins Omp10, Omp16, and Omp19 cross-reacted with O. anthropi and P. rubiacearum, which are actually the closest relatives of brucellae. Some of them also cross-reacted, but to a lower extent, with R. leguminosarum and A. tumefaciens. The putative Omp16 and Omp19 homologs in these bacteria showed the same apparent molecular masses as their Brucella counterparts. None of the antilipoprotein MAbs cross-reacted with Y. enterocolitica O:9, E. coli O:157, or S. urbana.  (+info)

Early acidification of phagosomes containing Brucella suis is essential for intracellular survival in murine macrophages. (3/532)

Brucella suis is a facultative intracellular pathogen of mammals, residing in macrophage vacuoles. In this work, we studied the phagosomal environment of these bacteria in order to better understand the mechanisms allowing survival and multiplication of B. suis. Intraphagosomal pH in murine J774 cells was determined by measuring the fluorescence intensity of opsonized, carboxyfluorescein-rhodamine- and Oregon Green 488-rhodamine-labeled bacteria. Compartments containing live B. suis acidified to a pH of about 4.0 to 4.5 within 60 min. Acidification of B. suis-containing phagosomes in the early phase of infection was abolished by treatment of host cells with 100 nM bafilomycin A(1), a specific inhibitor of vacuolar proton-ATPases. This neutralization at 1 h postinfection resulted in a 2- to 34-fold reduction of opsonized and nonopsonized viable intracellular bacteria at 4 and 6 h postinfection, respectively. Ammonium chloride and monensin, other pH-neutralizing reagents, led to comparable loss of intracellular viability. Addition of ammonium chloride at 7 h after the beginning of infection, however, did not affect intracellular multiplication of B. suis, in contrast to treatment at 1 h postinfection, where bacteria were completely eradicated within 48 h. Thus, we conclude that phagosomes with B. suis acidify rapidly after infection, and that this early acidification is essential for replication of the bacteria within the macrophage.  (+info)

Improved method for purification of bacterial DNA from bovine milk for detection of Brucella spp. by PCR. (4/532)

Different methods of extraction of bacterial DNA from bovine milk to improve the direct detection of Brucella by PCR were evaluated. We found that the use of a lysis buffer with high concentrations of Tris, EDTA, and NaCl, high concentrations of sodium dodecyl sulfate and proteinase K, and high temperatures of incubation was necessary for the efficient extraction of Brucella DNA. The limit of detection by PCR was 5 to 50 Brucella CFU/ml of milk.  (+info)

Outer membrane proteins Omp10, Omp16, and Omp19 of Brucella spp. are lipoproteins. (5/532)

The deduced sequences of the Omp10, Omp16, and Omp19 outer membrane proteins of Brucella spp. contain a potential bacterial lipoprotein processing sequence. After extraction with Triton X-114, these three proteins partitioned into the detergent phase. Processing of the three proteins is inhibited by globomycin, a specific inhibitor of lipoprotein signal peptidase. The three proteins were radioimmunoprecipitated from [(3)H]palmitic acid-labeled Brucella abortus lysates with monoclonal antibodies. These results demonstrate that Omp10, Omp16, and Omp19 are lipoproteins.  (+info)

Detection of antibodies to Brucella cytoplasmic proteins in the cerebrospinal fluid of patients with neurobrucellosis. (6/532)

The diagnosis of human neurobrucellosis usually relies on the detection of antibodies to Brucella lipopolysaccharide (LPS) in cerebrospinal fluid (CSF) by agglutination tests or enzyme-linked immunosorbent assay (ELISA). Here we describe the detection of immunoglobulin G (IgG) to cytoplasmic proteins (CP) of Brucella spp. by ELISA and Western blotting in seven CSF samples from five patients with neurobrucellosis. While IgG to CP (titers of 200 to 12, 800) and IgG to LPS (800 to 6,400) were found in the CSF of these patients, these antibodies were not detected in CSF samples from two patients who had systemic brucellosis without neurological involvement. The latter, however, had serum IgG and IgM to both LPS and CP. No reactivity to these antigens was found in CSF samples from 14 and 20 patients suffering from nonbrucellar meningitis and noninfectious diseases, respectively. These findings suggest that, in addition to its usefulness in the serological diagnosis of human systemic brucellosis, the ELISA with CP antigen can be used for the specific diagnosis of human neurobrucellosis.  (+info)

The 18-kDa cytoplasmic protein of Brucella species --an antigen useful for diagnosis--is a lumazine synthase. (7/532)

Previous studies have shown that the detection of antibodies to an 18-kDa cytoplasmic protein of Brucella spp. is useful for the diagnosis of human and animal brucellosis. This protein has now been expressed in recombinant form in Escherichia coli. The recombinant protein is soluble only under reducing conditions, but alkylation with iodoacetamide renders it soluble in non-reducing media. As shown by gel exclusion chromatography, this soluble form arranges in pentamers of 90 kDa. The reactivity of human and animal sera against the recombinant protein was similar to that found with the native protein present in brucella cytoplasmic fraction, suggesting that the recombinant protein is correctly folded. The protein has low but significant homology (30%) with lumazine synthases involved in bacterial riboflavin biosynthesis, which also arrange as pentamers. Biological tests on the crude extract of the recombinant bacteria and on the purified recombinant protein showed that the biological activity of the Brucella spp. 18-kDa protein is that of lumazine synthase. Preliminary crystallographic analysis showed that the Brucella spp. lumazine synthase arranges in icosahedric capsids similar to those formed by the lumazine synthases of other bacteria. The high immunogenicity of this protein, potentially useful for the design of acellular vaccines, could be explained by this polymeric arrangement.  (+info)

Competitive enzyme immunoassay for diagnosis of human brucellosis. (8/532)

The methods commonly used for human brucellosis serological testing are agglutination tests and the complement fixation test (CFT). Among the newer serological tests, primary binding assays were developed to improve sensitivity and specificity. The competitive enzyme immunoassay (CELISA) for the detection of serum antibody to Brucella is a multispecies assay which appears to be capable of differentiating vaccinal and cross-reacting antibodies from antibodies elicited by field infection in cattle. The competing monoclonal antibody used in this assay is specific for a common epitope of smooth lipopolysaccharide (S-LPS). In this study, we compared the CELISA to the classical tests for the diagnosis of human brucellosis. The CELISA cutoff value was determined to calculate its diagnostic specificity and sensitivity. A survey was performed with 911 sera. Of the sera, 341 were from an asymptomatic population that tested negative with conventional serological tests (screening and confirmatory). Based on these samples, the CELISA specificities were determined to be 99.7 and 100% with cutoff values of 28 and 30% inhibition (%I), respectively. In a further study with 393 additional sera from an asymptomatic population found negative by the conventional screening tests, the CELISA specificities were calculated to be 96.5 and 98.8% with cutoff values of 28 and 30%I. The CELISA sensitivities were determined to be 98.3 and 94.8% with cutoff values of 28 and 30%I, respectively, for sera from 116 individuals found positive by the classical tests. For the 51 culture-positive patients, CELISA was positive for 100%, the CFT was positive for 92%, and the standard tube agglutination test (TAT) was positive for 100%. The CELISA specificity was 100% for 31 sera from patients found negative by conventional serological tests but with brucellosis-like symptoms. The CELISA is fairly rapid to perform, somewhat faster than TAT, and cross-reacts less with other antigens (or antibodies) than the conventional tests. Further, the CELISA is simpler to perform that the CFT and may readily be standardized by the use of purified S-LPS antigen and monoclonal antibody for competition.  (+info)

The genus Brucella contains highly infectious species that are classified as biological threat agents. The timely detection and identification of the microorganism involved is essential for an effective response not only to biological warfare attacks but also to natural outbreaks. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) is a rapid method for the analysis of biological samples. The advantages of this method, compared to conventional techniques, are rapidity, cost-effectiveness, accuracy and suitability for the high-throughput identification of bacteria. Discrepancies between taxonomy and genetic relatedness on the species and biovar level complicate the development of detection and identification assays. In this study, the accurate identification of Brucella species using MALDI-TOF-MS was achieved by constructing a Brucella reference library based on multilocus variable-number tandem repeat analysis (MLVA) data. By comparing MS-spectra from Brucella
Curated}} {{Biorealm Genus}} [[Image:brucella_close_up.JPG,thumb,400px,right,Electron micrograph of B. abortus located inside cisternae of Vero cell. Within the perinuclear envelope, the cisternae containing B. abortus are discontinuously lined by ribosomes (arrows). From [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2114362 Detilleux et al.]]] ==Classification== ===Higher order taxa:=== Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Brucellaceae ===Species:=== Brucella abortus, Brucella canis, Brucella cetaceae, Brucella maris , Brucella melitensis, Brucella pinnipediae, Brucella sp. {, , height=10 bgcolor=#FFDF95 align=center , NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=234 Taxonomy] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=genomeprj&cmd=Search&dopt=DocSum&term=txid234%5BOrganism:exp%5D Genomes] ,} ==Description and Significance== Brucella sp. causes brucellosis, ...
An anti-Brucella vaccine candidate which comprises of purified Brucella lipopolysaccharide and cocktail of four Salmonella Typhimurium (ST)-Brucella vectors was reported earlier. Each vector constitutively express highly conserved Brucella antigens (rB), viz. lumazine synthase (BLS), proline racemase subunit A (PrpA), outer membrane protein-19 (Omp19), and Cu-Zn superoxide dismutase (SOD). The present study determined relative level of protection conferred by each single strain. Upon virulent challenge, the challenge strain was recovered most abundantly in non-immunized control mice and the ST-Omp19, ST-BLS, LPS, and ST-SOD immunized mice groups showed much less burden. Indirect ELISA based assay also confirmed the induction of antigen specific IgG for each antigen delivered. In route-wise comparison of the combined vaccine candidate, intraperitoneal (IP), intramuscular (IM), and subcutaneous (SC) immunizations revealed an indication of highly efficient routes for the protection. Splenocytes of ...
The patterns of susceptibility to hydrophobic and hydrophilic drugs and the uptake of the fluorescent probe N-phenyl-naphthylamine in Brucella spp., Haemophilus influenzae, Escherichia coli, and deep rough Salmonella minnesota mutants were compared. The results show that the outer membranes of smooth and naturally rough Brucella spp. do not represent barriers to hydrophobic permeants and that this absence of a barrier relates at least in part to the properties of Brucella lipopolysaccharide. ...
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Brucella Species answers are found in the Johns Hopkins ABX Guide powered by Unbound Medicine. Available for iPhone, iPad, Android, and Web.
LOCUS SBW13224.1 61 aa PRT BCT 14-DEC-2016 DEFINITION Brucella sp. 10RB9215 hypothetical protein protein. ACCESSION LT599047-28 PROTEIN_ID SBW13224.1 SOURCE Brucella sp. 10RB9215 ORGANISM Brucella sp. 10RB9215 Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Brucellaceae; Brucella; unclassified Brucella. REFERENCE 1 AUTHORS Blom J. JOURNAL Submitted (05-JUL-2016) to the INSDC. JLU, JLU, Justus-Liebig-University Giessen, Bioinformatics and Systems Biology, Heinrich-Buff-Ring 58, 35392 Giessen, 35390, Germany FEATURES Qualifiers source /organism=Brucella sp. 10RB9215 /chromosome=1 /host=African bull frog, Pyxicephalus edulis /isolate=BR10RB9215WGS1 /mol_type=genomic DNA /collection_date=2009 /db_xref=taxon:1149953 protein /transl_table=11 /locus_tag=BR10RB9215_C10028 /db_xref=UniProtKB/TrEMBL:A0A1M4L950 BEGIN 1 MEGQVIFYKN DRIIYRHHID VQEDDYSKGV NDALIAFQRN YAGFDLASDD IHIRFKKPGD 61 V ...
The primers for the genes of the identified proteins were designed and amplificated by PCR in four Brucella strains. The optimized PCR conditions and the length of PCR products were determined. The results of the PCR amplification in four different Brucella strains were shown. +, Corresponding sequence have been able to amplify; -, Corresponding sequence not been able to amplify ...
Published on 2014-03-05 00:00:00. Wattam AR, Foster JT, Mane SP, Beckstrom-Sternberg SM, Beckstrom-Sternberg JM, Dickerman AW, Keim P, Pearson T, Shukla M, Ward DV, Williams KP, Sobral BW, Tsolis RM, Whatmore AM, OCallaghan D (2014). Comparative phylogenomics and evolution of the brucellae: A path to virulence. Journal of Bacteriology, 196(5): 920-30. doi: 10.1128/JB.01091-13. PMID: 24336939. PMCID: PMC3957692.. Abstract. Brucella species include important zoonotic pathogens that have a substantial impact on both agriculture and human health throughout the world. Brucellae are thought of as stealth pathogens that escape recognition by the host innate immune response, modulate the acquired immune response, and evade intracellular destruction. We analyzed the genome sequences of members of the family Brucellaceae to assess its evolutionary history from likely free-living soil-based progenitors into highly successful intracellular pathogens. Phylogenetic analysis split the genus into two ...
Although shedding of zoonotic brucellae in milk has been demonstrated in organic hosts, these data are missing for the typical murine infection super model tiffany livingston even now. 30 bacteria/cell were within neutrophils and macrophages. As the bacterial matters in the spleen of lactating females verified a well-established an infection, only 50% from the pups harboured brucellae within their spleen, like the spleen of the uninfected pup given by an contaminated foster mother. To conclude, the murine style of infection might donate to a better knowledge of the zoonotic transmission of brucellosis. is in charge of the primary burden of disease in human beings. is principally isolated and sent from sheep and goats3. In the natural sponsor, brucellae have a predilection for both woman and male reproductive organs in sexually mature animals based on immune, hormonal and metabolic factors, including the availability of erythritol. This polyhydric alcohol can be found in high concentrations in ...
Brucella Series Symptom Relief kit is for the temporary relief of symptoms related to Brucella infection including fever, sweats, malaise, headache, back pain, neck pain, malodorous sweat, peculiar taste in mouth and gut inflammation.. A kit contains ten vials, each with a -different safe and -effective dilution of the actual deactivated virus or bacteria the kit is intended to address. The vials are taken in series, first increasing in potency for the first 30 days of the protocol, then decreasing in potency for the second 30 days to provide a full 60 days of symptom relief.. The basic 60-Day Series Protocol requires two ten-vial kits. Each vial is taken all at once, with the contents held under the tongue for 30 seconds. The first kit is taken over a 30-day period, one vial every three days, in ascending order (from #1 to #10). The second kit is then taken over 30 days (one vial every three days) in descending order (from vial #10 to vial #1).. This ascending and descending sequence provides ...
Objective To study antimicrobial resistance of Brucella melitenesis and instruct rational use of antimicrobial agents for clinic.Methods Bacteria were cultured and identified by Bact/Alert 3D and VITEK Ⅱ automicrobic system.Drug susceptibility was detected by E-test method.Results Four strains Brucella melitenesis were isolated from blood of 4 patients with fever and MIC of gentamicin,ampicillin and ciprofloxacin was(0.5)~(0.75 mg/L),(1.5)~(2.0 mg/L) and (4.0)~(8.0 mg/L) respectively.MIC of ceftazidime,amikacin and doxycycline were all above(8.0 mg/L).Conclusion The preferred drug for treating brucellosis is gentamicin or plus ampicillin or ciprofloxacin,or choosing antimicrobial agents according to antimicrobial susceptibility test results.
This test looks for brucellosis, an infectious disease usually caused by handling animals or milk products infected with the brucella bacteria.
This test looks for brucellosis, an infectious disease usually caused by handling animals or milk products infected with the brucella bacteria.
Interpretive Summary: Brucella are intracellular pathogens that cause reproductive losses in cattle and zoonotic infections in people. Live attenuated vaccines have been available for protecting domestic livestock against brucellosis for more than 60 years. Current vaccines are effective in preventing abortion and transmission of brucellosis, but poor at preventing infection or seroconversion. Current knowledge suggests that both the innate and adaptive immune responses contribute to immunity against intracellular pathogens and that binding of pathogen structures onto pattern recognition receptors (PMRs) is critical to development of protective immunity. The observations that killed bacteria or subunit vaccines do not appear to fully stimulate PMRs or mimic intracellular trafficking of live Brucella, may explain their inability to induce protection that equals that provided by live vaccines. Brucella appear to have multiple mechanisms that subvert innate and adaptive immunity and prevent or ...
Brucella bacteria bacteria are found in cattle, pigs, goats, sheep, working dogs and domestic animals mainly overseas - risk to travellers
Brucellosis is primarily a worldwide zoonotic disease caused by Brucella species. The genus Brucella contains highly infectious species that are classified ...
Kang, Yoon-Suk, Daniel A. Brown, and James E. Kirby. Brucella neotomae Recapitulates Attributes of Zoonotic Human Disease in a Murine Infection Model. Infection and Immunity 87.1 (2019): e00255-18. Web. 08 April. 2020. ...
When and how often laboratory tests are done may depend on many factors. The timing of laboratory tests may rely on the results or completion of other tests, procedures, or treatments. Lab tests may be performed immediately in an emergency, or tests may be delayed as a condition is treated or monitored. A test may be suggested or become necessary when certain signs or symptoms appear. Due to changes in the way your body naturally functions through the course of a day, lab tests may need to be performed at a certain time of day. If you have prepared for a test by changing your food or fluid intake, lab tests may be timed in accordance with those changes. Timing of tests may be based on increased and decreased levels of medications, drugs or other substances in the body. The age or gender of the person being tested may affect when and how often a lab test is required. Chronic or progressive conditions may need ongoing monitoring through the use of lab tests. Conditions that worsen and improve may ...
PCR-RFLP analysis of Brucella LPS genes manAO-Ag, manBO-Ag, wbkD, wbkF, wboA and wa**. Panel A. Lanes: 1, molecular size markers; 2, manAO-Ag from B. melitensis
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This is the microbiology questions and answers section on Brucella with explanation for various interview, competitive examination and entrance test. Solved examples with detailed answer description, explanation are given and it would be easy to understand.
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Spleen cells from mice infected with the rough Brucella melitensis strain B115 were fused with NSO myeloma cells. Hybridoma supernatants were screened in ELISA with cell walls (CW), sonicated cell extracts (CE) and rough lipopolysaccharide (R-LPS) of B. melitensis strain B115 and whole B. melitensis B115 cells. Surprisingly, 22 monoclonal antibodies (mAbs) reacting in ELISA with both CW and CE but not with R-LPS and bacterial cells were shown by immunoblot analysis and ELISA to react with smooth lipopolysaccharide (S-LPS). These mAbs also reacted in ELISA with O polysaccharides (OPS) from the smooth Brucella abortus strain 99 and the smooth B. melitensis strain 16M and thus recognize epitopes present on the O-chain. Proteinase K LPS preparations from B. melitensis B115 analysed by immunoblotting with one mAb (12G12) recognizing S-LPS of both A and M specificity displayed the typical S-LPS high-molecular-mass ladder pattern but no S-LPS was detected in the phenol/water/chloroform/light petroleum LPS
Synonyms for Brucella canis in Free Thesaurus. Antonyms for Brucella canis. 1 word related to Brucella: coccobacillus. What are synonyms for Brucella canis?
The foodproof Brucella Detection Kit detects the genus Brucella and additionally identifies the two most common food-associated disease species Brucella abortus and Brucella melitensis. The rod-shaped, Gram-negative, aerobic bacteria are found in cows, sheep and pigs. The bacteria can be transmitted to humans through raw milk or meat causing brucellosis, also called Malta Fever.
Pathogens infecting mammalian cells have developed various strategies to suppress and evade their hosts defensive mechanisms. In this line, the intracellular bacteria that are able to survive and propagate within their host cells must have developed strategies to avert their hosts killing attitude. Studying the interface of host-pathogen confrontation can provide valuable information for defining therapeutic approaches. Brucellosis, caused by the Brucella strains, is a zoonotic bacterial disease that affects thousands of humansand animals around the world inflicting discomfort and huge economic losses. Similar to many other intracellular dwelling bacteria, infections caused by Brucella are difficult to treat, and hence any attempt at identifying new and common therapeutic targets would prove beneficial for the purpose of curing infections caused by the intracellular bacteria. In THP-1 macrophage infected with Brucella melitensis we studied the expression levels of four hosts genes, i.e. EMP2, ST8SIA4
To analyze the strategy adopted by Brucella to develop within mononuclear cells, we examined whether Brucellainfection was able to positively or negatively modulate apoptosis in human monocytic phagocytes. The results of various analyses (DNA fragmentation, microscopic analysis, and flow cytometry analysis) showed that Brucella infection inhibited apoptosis which spontaneously occurs in human monocytes in the absence of an activation signal and rendered macrophagic cells resistant to apoptosis induced by immunological factors.. Although the Brucella-mediated effect on monocyte apoptosis was somewhat similar to the E. coli LPS effect (references24 and 28 and our results), convergent data demonstrated that Brucella LPS cannot account for the antiapoptotic properties of the live bacteria. (i) Experiments performed in the presence of neutralizing anti-TNF-α Ab showed that TNF-α, which plays a central role in Brucella(or E. coli) LPS-induced protection of monocytes, did not participate in the ...
Looking for online definition of brucellae in the Medical Dictionary? brucellae explanation free. What is brucellae? Meaning of brucellae medical term. What does brucellae mean?
Brucellosis is a contagious disease of animals that also affects humans. The disease is also known as Bangs Disease. In humans, its known as Undulant Fever.. Brucellosis is one of the most serious diseases of livestock, considering the damage done by the infection in animals. Decreased milk production, weight loss, loss of young, infertility, and lameness are some of the affects on animals.. The Brucella species are named for their primary hosts: Brucella melitensis is found mostly is goats,sheep and camels, B. abortus is a pathogen of cattle, B. suis is found primarily in swine and B. canis is found in dogs.. There are two common ways people get infected with brucellosis. First, individuals that work with infected animals that have not been vaccinated against brucellosis. This would include farmers, slaughterhouse workers and veterinarians.. Related: College microbiology classes linked to Salmonella Typhimurium outbreak. They get infected through direct contact or aerosols produced by the ...
The current investigation is associated with the third known case of brucellosis from Brucella RB51 in a New York resident who who drank raw milk purchased from Millers Biodiversity Farm in Quarryville, Pennsylvania, was diagnosed with brucellosis in November 2018.. Milk samples from the dairy tested positive for Brucella strain RB51. People who consumed raw milk or raw milk products from this dairy since January 2016 may have been exposed.. As of January 22, 2019, exposures have been identified in 19 states: Alabama, California, Connecticut, Florida, Georgia, Iowa, Maryland, Massachusetts, Michigan, Minnesota, Mississippi, New Jersey, New York, North Carolina, Ohio, Pennsylvania, Rhode Island, South Carolina, and Virginia.. CDC notes that Brucella strain RB51 is a live-attenuated cattle vaccine strain, which can be shed in milk and can cause infections in humans. RB51 is resistant to rifampin and penicillin. There is no serological test available to detect RB51 infection. Blood culture is the ...
Rabbit polyclonal antibody raised against full length recombinant Brucella suis vjbR. Recombinant protein corresponding to full length Brucella suis vjbR. (PAB16064) - Products - Abnova
We report a case of Brucella abortus-induced brucellosis that displayed high hepatic transaminases similar to that in acute hepatitis, fourth month relapsed following doxycycline-streptomycin treatment, and developed doxycycline-related hepatotoxicity in second treatment. Brucella hepatitis may occur with various Brucella spp. and may relapse even following ...
Brucella species are small, coccobacillary, Gram-negative rods that morphologically resemble Haemophilus and Bordetella. They are nonmotile, non-acid-fast, and non-spore-forming. The cells have a typical Gram-negative structure, and the outer membrane contains proteins. The genus Brucella contains nine closely related variants that differ primarily in their preferred terrestrial or marine hosts. Taxonomists vacillate as to whether they should be called species or something else. The three most commonly infecting humans, B abortus (cattle), B melitensis (sheep, goats), and B suis (swine), will all be referred to here as Brucella abortus or simply Brucella ...
Using serology and bacterial culture, we determined the prevalence of Brucella spp. and the antibody to Brucella spp. in a feral swine (Sus scrofa) population in proximity to a cattle herd that was culture positive for Brucella abortus and Brucella suis in north-central Texas, USA. During a prospective cross-sectional quantitative study in April 2005, we collected blood and tissue samples from 40 feral swine within a 30-km radius of the infected herd. Serum samples were tested by the Rose Bengal test, particle concentration fluorescence immunoassay, and fluorescence polarization assay. In addition, tissue samples were cultured, and the Brucella species and biovar determined. Four feral swine were Brucella positive by serology, and two were culture positive for B. suis biovar 1. Of the culture-positive swine, one was concurrently antibody and culture positive, and one was culture positive only. The presumptive source of the B. suis infection in the index cattle herd was likely the surrounding feral swine
In 1999, a researcher suffered headaches, lassitude and a severe sinusitis after exposure to marine mammal strains of Brucella with which the worker was in contact. The symptoms resolved in one week after treatment with doxycycline and rifampin. The researcher had a positive titer for Brucella, and the organism was cultured from blood samples. PCR-RFLP was used to positively identify the isolates as being comparable to marine mammal Brucella (Brew and Staunton 1999). Organisms Brucella ssp. are gram-negative intracellular bacteria and are a major source of zoonoses worldwide. B. melitensus, B. abortus, and B. suis are some species commonly recognized to play a role in human and animal health. Nomenclature for marine mammal strains of Brucella has not yet been fully developed, but B. maris and B. pinnipedia have so far been named. Transmission occurs primarily through contact with aborted fetal material, and consumption of contaminated milk. ...
What is brucellosis? Brucellosis is an infectious disease caused by the bacteria of the genus Brucella. These bacteria are primarily passed among animals, and they cause disease in many different vertebrates. Various Brucella species affect sheep, goats, cattle, deer, elk, pigs, dogs, and several other animals. Humans become infected by coming in contact with animals or animal products that are contaminated with these bacteria. In humans brucellosis can cause a range of symptoms that are similar to the flu and may include fever, sweats, headaches, back pains, and physical weakness. Severe infections of the central nervous systems or lining of the heart may occur. Brucellosis can also cause long-lasting or chronic symptoms that include recurrent fevers, joint pain, and fatigue.. Back to Top How common is brucellosis? Brucellosis is not very common in the United States, where100 to 200 cases occur each year. But brucellosis can be very common in countries where animal disease control programs have ...
Find details on Brucella canis in dogs including diagnosis and symptoms, active forms, resting forms, clinical effects, treatment and more. All information is peer reviewed.
Brucella canis is a Gram-negative proteobacterium in the family Brucellaceae that causes brucellosis in dogs and other canids. B. canis is rod-shaped or a coccus, and is oxidase, catalase, and urease positive. The species was firstly described in United States in 1966 where mass abortions of beagles were documented. The disease is characterized by epididymitis and orchitis in male dogs, endometritis, placentitis, and abortions in females, and often presents as infertility in both sexes. Other symptoms such as inflammation in the eyes and axial and appendicular skeleton; lymphadenopathy and splenomegaly, are less common. Humans can be also infected, but occurrences are rare. B. canis is a zoonotic organism. Signs of this disease are different in both genders of dogs; females that have B. canis infections face an abortion of their developed fetuses. Males face the chance of infertility, because they develop an antibody against the sperm. This may be followed by inflammation of the testes which ...
La mayoría de las infecciones bacterianas inducen la activación de los neutrófilos polimorfonucleares (PMN), mejoran su función microbicida y promueven la supervivencia de estos leucocitos durante períodos prolongados. Brucella abortus es un patógeno sigiloso que evade la inmunidad innata, apenas activa los PMN y resiste los mecanismos de destrucción de estos fagocitos. Los intrigantes signos clínicos que se observan durante la brucelosis son el bajo número de PMN infectados por Brucella en los órganos diana y la neutropenia en una proporción de los pacientes; características que merecen una mayor atención. Aquí demostramos que B.abortus mata prematuramente a los PMN humanos de forma dosis dependiente y específica de la célula. La muerte de los PMNs es concomitante con la liberación de lipopolisacáridos intracelulares de Brucella (Br-LPS) en vacuolas. Esta molécula y su lípido A reproducen la muerte celular prematura de los PMN, un fenómeno asociado a la baja producción de ...
Brucella, a Gram-negative bacterium, is classified as a potential bioterrorism agent mainly due to the low dose needed to cause infection and the ability to transmit the bacteria via aerosols. Goats/sheep, cattle, pigs, dogs, sheep and rodents are infected by B. melitensis, B. abortus, B. suis, B. canis, B. ovis and B. neotomae, respectively, the six classical Brucella species. Most human cases are caused by B. melitensis and B. abortus. Our aim was to specifically detect Brucellae with smooth lipopolysaccharide (LPS) using a highly sensitive monoclonal antibody (mAb) based immunological assay. To complement molecular detection systems for potential bioterror agents, as required by international biodefense regulations, sets of mAbs were generated by B cell hybridoma technology and used to develop immunological assays. The combination of mAbs most suitable for an antigen capture assay format was identified and an immunoassay using the Luminex xMAP technology was developed.
Neutralophilic bacteria have developed specific mechanisms to cope with the acid stress encountered in environments such as soil, fermented foods- and host compartments. In Escherichia coli, the glutamate decarboxylase (Gad)-dependent system is extremely efficient: it requires the concerted action of glutamate decarboxylase (GadA/GadB) and of the glutamate/GABA antiporter, GadC. Notably, this system is operative also in new strains/species of Brucella, among which Brucella microti, but not in the
Oceans and seas are the widest sources of biological and chemical diversity representing a prolific reserve of unexploited and/or unknown microorganisms [1, 2]. Thus, marine environments are great resources of new substances having potential applications in pharmaceutical, feed and food, fine chemicals and enzyme industries [2, 3]. The search of new microorganisms, having unique physiological and metabolic capabilities, aids to better comprehend the ecosystem and provides opportunities to discover new compounds of commercial importance. This is particularly true for marine bacteria that have been less studied than their terrestrial counterpart and are often underrated or completely ignored by many scientists [4, 5].. Among the microbial products, exopolysaccharides (EPS) have many important physiological functions and various practical applications deductible from their roles in nature.. These high molecular weight polymers represent essential components of the secreted extracellular material ...
Recent studies show that FDG-PET may contribute for precise grading and prognosis in different solid tumors, including soft tissue sarcomas [5], as high grade tumors show a much higher uptake of 18F-FDG due to a higher glycolytic phenotype [3]. Also, it was recently described that FDG-PET should be considered an important imaging modality for therapeutic monitoring in patients with high-grade STS [6, 7]. In this context, metabolic characterization of STS emerges as a possibly relevant approach for STS management, with therapeutic implications as early treatment decisions such as discontinuation of chemotherapy in non-responding patients could be based on FDG-PET criteria [7]. Importantly, the hyperglycolytic phenotype present in this type of tumors, similarly to other solid tumors, may be the basis for the use of new directed therapeutic strategies which are currently in clinical trials [29]. Therefore, tumor metabolic characterization, including MCTs as responsible for lactate efflux from ...
Brucellosis is one of the most widespread zoonosis in the world. In China, 90% of human brucellosis occurs in six northern agricultural provinces. However, there is a recent increase in the trend of human brucellosis in southern provinces with limited cases reported in the literature. Our study aims to describe the clinical features and epidemiology of brucellosis in a tertiary hospital in southern China. A retrospective case series of brucellosis was conducted between January 1, 2014 and October 31. 2018. Cases were identified based on positive Brucella serology by tube agglutination test, or positive culture from clinical specimen identified by Vitek 2 and MALDL-TOF MS. Clinical details of brucellosis including patients occupation, risk factors, and complications were analyzed. Clinical characteristics between patients from Guangdong and other provinces were also compared. A total of 13 cases of laboratory-confirmed brucellosis were identified. 7 (53.8%) of the patients were male, 6 (46.2%) were
Abstract of Paper: Serodiagnosis Of Human Brucellosis : Validity Of Brucella-Specific IgM Antibodies Dipstick Test As A Screening Modality Of Febrile Patients , Author: Hosam Armen ; Mohamed El-Ass& & Jehan H. Sabry
Background: Brucellosis is the most common zoonotic infection over the world, caused by bacterial genus brucella. The disease is transmitted rarely via human-to-human transmission. Limited data support vertical transmission of human brucellosis. Herein, we reported a case of c...
Brucellosis is a zoonotic bacterial disease caused by Brucella spp. The virulence of these bacteria is dependent on their ability to invade and replicate within host cells. In a previous study, a putative gene bab_RS27735 encoding an amino acid ABC transporter substrate-binding protein homologous to AapJ protein was found to be involved in Brucella abortus virulence. In this study, we successfully constructed a bab_RS27735 deletion mutant, Δ27735. Compared with the wild-type strain, the lipopolysaccharide pattern of the mutant was not changed, but the growth ability was slightly defected in the exponential phase. In tolerance tests, sensitivity of the Δ27735 mutant to oxidative stress, bactericidal peptides or low pH was not different from that of the wild-type strain. Cell infection assay showed that the mutant was reduced survival within macrophages but could efficiently escape lysosome degradation. The results of a virulence test showed that the Δ27735 mutant was attenuated in a mouse model at the
Sentinel laboratory guidelines for suspected agents of bioterrorism: species. Ariza, J, Bosch, J, Gudiol, F. Relevance of in vitro antimicrobial susceptibility of to relapse rate in human brucellosis. Antimicrob Agents Chemother. vol. 30. 1986. pp. 958-60. (In the context of a clinical trial of tetracycline/streptomycin, doxycycline/streptomycin and doxycycline/rifampin, pre-treatment minimum inhibitory concentration (MIC) of the drugs given did not predict the risk of relapse, and the MICs of relapse-associated isolates did not differ significantly from pre-treatment.) Ariza, J, Bosilkovski, M, Cascio, A. Perspectives for the treatment of Brucellosis in the 21st century: the Ioannina recommendations. PLoS Med. vol. 4. 2007. pp. e317(Expert panel recommendations for the treatment of uncomplicated Brucellosis. Doxycycline/gentamicin regarded as gold standard [good evidence, high quality], doxycycline/rifampin as slightly inferior but more convenient [good evidence, high quality], ...
Sentinel laboratory guidelines for suspected agents of bioterrorism: species. Ariza, J, Bosch, J, Gudiol, F. Relevance of in vitro antimicrobial susceptibility of to relapse rate in human brucellosis. Antimicrob Agents Chemother. vol. 30. 1986. pp. 958-60. (In the context of a clinical trial of tetracycline/streptomycin, doxycycline/streptomycin and doxycycline/rifampin, pre-treatment minimum inhibitory concentration (MIC) of the drugs given did not predict the risk of relapse, and the MICs of relapse-associated isolates did not differ significantly from pre-treatment.). Ariza, J, Bosilkovski, M, Cascio, A. Perspectives for the treatment of Brucellosis in the 21st century: the Ioannina recommendations. PLoS Med. vol. 4. 2007. pp. e317(Expert panel recommendations for the treatment of uncomplicated Brucellosis. Doxycycline/gentamicin regarded as gold standard [good evidence, high quality], doxycycline/rifampin as slightly inferior but more convenient [good evidence, high quality], ...
Brucella BvrR protein: BvrR/BvrS represent the two-component system involved in the regulation of Brucella virulence; amino acid sequence in first source
Introduction & Objective: Brucellosis is an important zoonosis considered a serious hazard to public health . Human brucellosis is caused by one of the four species of the brucella genus: B. melitensis is principally found in goats and sheep, B. abortus in cattle, B. suis in swine and B. canis in dogs. ...
Brucellosis is a common bacterial zoonotic infection but data on the prevalence among humans and animals is limited in Kenya. A cross-sectional survey was conducted in three counties practicing different livestock production systems to simultaneously assess the seroprevalence of, and risk factors for brucellosis among humans and their livestock (cattle, sheep, camels, and goats). A two-stage cluster sampling method with random selection of sublocations and households was conducted. Blood samples were collected from humans and animals and tested for Brucella immunoglobulin G (IgG) antibodies. Human and animal individual seroprevalence was 16% and 8%, respectively. Household and herd seroprevalence ranged from 5% to 73% and 6% to 68%, respectively. There was a 6-fold odds of human seropositivity in households with a seropositive animal compared with those without. Risk factors for human seropositivity included regular ingestion of raw milk (adjusted odds ratio [aOR] = 3.5, 95% confidence interval ...
Brucella abortus causes brucellosis mainly in cattle. The infection is transmitted to humans by ingestion of animal products or direct contact with infected material. While the intracellular lifestyle of Brucella is well characterized, its extracellular survival is poorly understood. In nature, bacterial persistence is associated with biofilms, where aggregated cells are protected from adversity. The inability of Brucella abortus to aggregate under aerobiosis and that fact that the replicative niche of Brucella is characterized by microaerobic conditions prompted us to investigate the capacity of this pathogen to aggregate and grow in biofilms under microaerobiotic conditions. The results show that B. abortus aggregates and produces biofilms. The aggregates tolerate desiccation better than planktonic cells do, adhere and displace even in the absence of the lipopolysaccharide-O antigen, flagella, the transcriptional regulator VjbR, or the enzymes that synthesize, transport, and modify cyclic β ...
Among animals, brucellosis is transmitted directly (contact with placenta, aborted fetus, the liquor , vaginal and/or uterus secretion) or indirectly (if infected and non-infected health herds mix while grazing or by contact with environment in which the bacteria can be found such as manure, pastures, water, etc). Most infected male animals continue to secrete Brucella via semen for the lifetime. Presence of infectious agents in udder and surrounding lymph nodes results in permanent or occasional secretion of Brucella via milk and as such it is a serious source of infection for young animals which can also be infected via uterus. In infected lambs and young goats, Brucella inhabits lymph nods of bowls and can be secreted out of their body via droppings. Infected young cattle develop the disease once they reached sexual maturity. Sheppard dogs can also spread brucellosis between ruminants but they can also get ill as well. In high humidly, low temperature and unsunny weather conditions, Brucella ...
Symptomatic seropositive family members were treated, whereas asymptomatic seropositive family members were followed-up with no treatment unless they showed symptoms and an increase in STA titers. Of eight family members followed, two were diagnosed with acute brucellosis.. Discussion. Turkey has seven geographical regions and is an important migration route between Europe and Asia. There are significant differences between regions, in terms of geographical features and economic and social development. Due to the geographic situation, Turkey is a risk factor for many infectious diseases, such as brucellosis.11 A total of 189,226 cases of human brucellosis were officially reported between 2000 and 2005, of which approximately 90,000 were registered (approximately 15,000 cases per year) in Turkey. The highest seroprevalence was observed in the eastern and southeastern regions of the country.12 Despite being endemic in Turkey, brucellosis remains underdiagnosed due to its non-specific clinical ...
ID BRUMB_1_PE1003 STANDARD; PRT; 44 AA. AC BRUMB_1_PE1003; C0RJ20; DT 00-JAN-0000 (Rel. 1, Created) DT 00-JAN-0000 (Rel. 2, Last sequence update) DT 00-JAN-0000 (Rel. 3, Last annotation update) DE SubName: Full=Putative uncharacterized protein; (BRUMB_1.PE1003). GN OrderedLocusNames=BMEA_A1086; OS BRUCELLA MELITENSIS ATCC 23457. OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Brucellaceae; OC Brucella. OX NCBI_TaxID=546272; RN [0] RP -.; RG -.; RL -.; CC -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS BRUMB_1.PE1003. CC Brucella melitensis ATCC 23457 chromosome chromosome I, complete sequen CC sequence. CC -!- ANNOTATIONS ORIGIN:C0RJ20_BRUMB CC -!- GENE_FAMILY: HBG000000000 [ FAMILY / ALN / TREE ] DR UniProtKB/Swiss-Prot; C0RJ20; -. DR EMBL; CP001488; ACO00828.1; -; Genomic_DNA. DR RefSeq; YP_002732782.1; NC_012441.1. DR GeneID; 7677846; -. DR GenomeReviews; CP001488_GR; BMEA_A1086. DR KEGG; bmi:BMEA_A1086; -. DR ProtClustDB; CLSK2476470; -. DR HOGENOMDNA; BRUMB_1.PE1003; -. ...
Fingerprint Dive into the research topics of Intratumoral Agreement of High-Resolution Magic Angle Spinning Magnetic Resonance Spectroscopic Profiles in the Metabolic Characterization of Breast Cancer. Together they form a unique fingerprint. ...
High Resolution Magic Angle Spinning (HR-MAS) NMR allows metabolic characterization of biopsies. HR-MAS spectra from tissues of most organs show strong lipid contributions that are overlapping metabolite regions, which hamper metabolite estimation. Metabolite quantification and analysis would benefit from a
Brucella spp. are zoonotic Gram-negative intracellular pathogens with the ability to survive and replicate in phagocytes. It has been shown that bacterial proteins expressed abundantly in this niche are stress-related proteins capable of triggering effective immune responses. BMEI1549 is a molecular chaperone designated DnaK that is expressed under stress conditions and helps to prevent formation of protein aggregates. In order to study the potential of DnaK as a prospective Brucella subunit vaccine, immunogenicity and protective efficacy of recombinant DnaK from Brucella melitensis was evaluated in BALB/c mice. The dnak gene was cloned, expressed in Escherichia coli, and the resulting recombinant protein used as subunit vaccine. DnaK-immunized mice showed a strong lymphocyte proliferative response to in vitro antigen stimulation. Although comparable levels of antigen-specific IgG2a and IgG1 were observed in immunized mice, high amounts of IFN-?, IL-12 and IL-6, no detectable level of IL-4 and ...
Lineage: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Brucellaceae; Brucella; Brucella abortus; Brucella abortus bv. ...
Brucellosis is an infectious disease caused the Brucella bacteria which spread to humans from animals. Some Brucella bacteria strains are seen in cows, while some occur in pigs, dogs, sheep, camels and goats. This is the forum for discussing anything related to this health condition
On April 23, 2011, plaintiff Christine Brucella, 77, a retiree, alleged she was walking down an aisle at Shoprite supermarket in Paramus when another shopper struck her with his shopping cart, causing her to fall. Brucella claimed she sustained a fracture to her right hip in the incident. Brucella sued Shoprite of Paramus and its buying cooperative, Wakefern Food Corporation, for negligence. She claimed the aisle was too narrow to allow her to move out of the way of the shopping cart to avoid being hit by it. Plaintiffs counsel claimed the aisle created a dangerous condition for patrons who are walking when a shopper pushing a cart suddenly appears in front them. The plaintiffs liability expert testified that the aisle needed to be 12 feet wide to assure safe passage of patrons with shopping carts. The defense argued that the aisle was the appropriate size and was not a dangerous condition. Defense counsel noted that the accident was captured on surveillance video. Counsel contended that ...
Doctors usually confirm a diagnosis of brucellosis by testing blood or bone marrow for the brucella bacteria or by testing blood for antibodies to the bacteria. To help detect complications of brucellosis, your doctor may order additional tests, including: X-rays. X-rays can reveal changes in your bones and joints ...
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The innate immune system detects the presence of microbes in tissue by pattern recognition of conserved microbial structures, known as pathogen-associated molec...
Investigations in Texas and New Jersey identified people infected with a dangerous germ called Brucella RB51. If not treated with antibiotics, Brucella RB51 infection can lead to serious chronic infections and complications. These illnesses occurred among people who drank raw (unpasteurized) milk from a dairy farm in Texas and a milk delivery service in the Northeast. CDC and state health officials warned customers not to drink raw milk from these dairies and advised those who consumed the raw milk to seek antibiotic treatment.. ...
Size, charge and structural heterogeneity of Brucella abortus lipopolysaccharides demonstrated by two-dimensional gel electrophoresis (pages 283-288). Dr. Blair A. Sowa, Richard P. Crawforda, Fred C. Heck, John D. Williams, Albert M. Wu, Katherine A. Kelly and L. Garry Adams. Version of Record online: 16 APR 2008 , DOI: 10.1002/elps.1150070608. ...
For 29er tires, more than 100g per wheel can be saved with Tubo tubes compared to standard butyl tubes-and almost twice as much space when packaged. Their riding safety is twice as high as for standard tubes and offers maximum protection against a breakdown. The assembly is just as easy as standard tubes. Tubolito tubes are also a lightweight alternative to a tubeless setup. - 42mm valve ...
Antibiotic activity of molds for Brucella. The isolation of two active substances from a strain of Aspergillus terreus Thom: one in crystalline form, the other as an amorphous concentrate ...
Brucella melitensis is the cause of undulant fever in man and of abortion in goats. It is usually transmitted by goat's milk. ... Brucella is the genus and Brucellaceae is the family of the bacteria which was named after him in recognition of his ... later renamed Brucella melitensis). Themistocles Zammit, one of the members of the commission, discovered the carrier of the ... http://www.royalsoced.org.uk/cms/files/fellows/biographical_index/fells_indexp1.pdf Corbel, M.J.; Banai, M. (2015). "Brucella ...
Brucella spp. can remain latent in a macrophage via inhibition of phagosome-lysosome fusion; causes brucellosis (undulant fever ...
A urinary tract infection (UTI) is an infection that affects part of the urinary tract.[1] When it affects the lower urinary tract it is known as a bladder infection (cystitis) and when it affects the upper urinary tract it is known as a kidney infection (pyelonephritis).[9] Symptoms from a lower urinary tract infection include pain with urination, frequent urination, and feeling the need to urinate despite having an empty bladder.[1] Symptoms of a kidney infection include fever and flank pain usually in addition to the symptoms of a lower UTI.[9] Rarely the urine may appear bloody.[6] In the very old and the very young, symptoms may be vague or non-specific.[1][10] The most common cause of infection is Escherichia coli, though other bacteria or fungi may rarely be the cause.[2] Risk factors include female anatomy, sexual intercourse, diabetes, obesity, and family history.[2] Although sexual intercourse is a risk factor, UTIs are not classified as sexually transmitted infections (STIs).[11] ...
Traditionally, gonorrhea was diagnosed with Gram stain and culture; however, newer polymerase chain reaction (PCR)-based testing methods are becoming more common.[16][28] In those failing initial treatment, culture should be done to determine sensitivity to antibiotics.[29] Tests that use polymerase chain reaction (PCR, aka nucleic acid amplification) to identify genes unique to N. gonorrhoeae are recommended for screening and diagnosis of gonorrhea infection. These PCR-based tests require a sample of urine, urethral swabs, or cervical/vaginal swabs. Culture (growing colonies of bacteria in order to isolate and identify them) and Gram-stain (staining of bacterial cell walls to reveal morphology) can also be used to detect the presence of N. gonorrhoeae in all specimen types except urine.[30][31] If Gram-negative, oxidase-positive diplococci are visualized on direct Gram stain of urethral pus (male genital infection), no further testing is needed to establish the diagnosis of gonorrhea ...
The word cholera is from Greek: χολέρα kholera from χολή kholē "bile". Cholera likely has its origins in the Indian subcontinent as evidenced by its prevalence in the region for centuries.[14] The disease appears in the European literature as early as 1642, from the Dutch physician Jakob de Bondt's description it in his De Medicina Indorum.[82] (The "Indorum" of the title refers to the East Indies. He also gave first European descriptions of other diseases.) Early outbreaks in the Indian subcontinent are believed to have been the result of poor living conditions as well as the presence of pools of still water, both of which provide ideal conditions for cholera to thrive.[83] The disease first spread by trade routes (land and sea) to Russia in 1817, later to the rest of Europe, and from Europe to North America and the rest of the world,[14] (hence the name "Asiatic cholera"[1]). Seven cholera pandemics have occurred in the past 200 years, with the seventh pandemic originating in ...
Dysentery is initially managed by maintaining fluid intake using oral rehydration therapy. If this treatment cannot be adequately maintained due to vomiting or the profuseness of diarrhea, hospital admission may be required for intravenous fluid replacement. Ideally, no antimicrobial therapy should be administered until microbiological microscopy and culture studies have established the specific infection involved. When laboratory services are not available, it may be necessary to administer a combination of drugs, including an amoebicidal drug to kill the parasite and an antibiotic to treat any associated bacterial infection. Anyone with bloody diarrhea needs immediate medical help. Treatment often starts with an oral rehydrating solution-water mixed with salt and carbohydrates-to prevent dehydration. (Emergency relief services often distribute inexpensive packets of sugars and mineral salts that can be mixed with clean water and used to restore lifesaving fluids in dehydrated children gravely ...
... (TD) is a stomach and intestinal infection. TD is defined as the passage of unformed stool (one or more by some definitions, three or more by others) while traveling.[2][3] It may be accompanied by abdominal cramps, nausea, fever, and bloating.[3] Occasionally bloody diarrhea may occur.[5] Most travelers recover within four days with little or no treatment.[3] About 10% of people may have symptoms for a week.[3] Bacteria are responsible for more than half of cases.[3] The bacteria enterotoxigenic Escherichia coli (ETEC) are typically the most common except in Southeast Asia, where Campylobacter is more prominent.[2][3] About 10% to 20% of cases are due to norovirus.[3] Protozoa such as Giardia may cause longer term disease.[3] The risk is greatest in the first two weeks of travel and among young adults.[2] People affected are more often from the developed world.[2] Recommendations for prevention include eating only properly cleaned and cooked food, drinking bottled water, and ...
Protective levels of anticapsular antibodies are not achieved until 7-14 days following administration of a meningococcal vaccine, vaccination cannot prevent early onset disease in these contacts and usually is not recommended following sporadic cases of invasive meningococcal disease. Unlike developed countries, in sub-Saharan Africa and other under developed countries, entire families live in a single room of a house.[21][22] Meningococcal infection is usually introduced into a household by an asymptomatic person. Carriage then spreads through the household, reaching infants usually after one or more other household members have been infected. Disease is most likely to occur in infants and young children who lack immunity to the strain of organism circulating and who subsequently acquire carriage of an invasive strain.[23] By preventing susceptible contacts from acquiring infection by directly inhibiting colonization. Close contacts are defined as those persons who could have had intimate ...
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... is a large family of Gram-negative bacteria. It was first proposed by Rahn in 1936, and now includes over 30 genera and more than 100 species. Its classification above the level of family is still a subject of debate, but one classification places it in the order Enterobacterales of the class Gammaproteobacteria in the phylum Proteobacteria.[2][3][4][5] Enterobacteriaceae includes, along with many harmless symbionts, many of the more familiar pathogens, such as Salmonella, Escherichia coli, Klebsiella, and Shigella. Other disease-causing bacteria in this family include Enterobacter and Citrobacter. Members of the Enterobacteriaceae can be trivially referred to as enterobacteria or "enteric bacteria",[6] as several members live in the intestines of animals. In fact, the etymology of the family is enterobacterium with the suffix to designate a family (aceae)-not after the genus Enterobacter (which would be "Enterobacteraceae")-and the type genus is Escherichia. ...
... is one of three types of plague caused by the plague bacterium (Yersinia pestis).[1] One to seven days after exposure to the bacteria, flu-like symptoms develop.[1] These symptoms include fever, headaches, and vomiting,[1] as well as swollen and painful lymph nodes occur in the area closest to where the bacteria entered the skin.[2] Occasionally, the swollen lymph nodes, known as "buboes" pictured to the right, may break open.[1] The three types of plague are the result of the route of infection: bubonic plague, septicemic plague, and pneumonic plague.[1] Bubonic plague is mainly spread by infected fleas from small animals.[1] It may also result from exposure to the body fluids from a dead plague-infected animal.[5] Mammals such as rabbits, hares, and some cat species are susceptible to bubonic plague, and typically die upon contraction.[6] In the bubonic form of plague, the bacteria enter through the skin through a flea bite and travel via the lymphatic vessels to a lymph node, ...
... are a class of gram-negative bacteria, and one of the eight classes of the phylum Proteobacteria.[1] The Betaproteobacteria are a class comprising over 75 genera and 400 species of bacteria.[2] Together, the Betaproteobacteria represent a broad variety of metabolic strategies and occupy diverse environments from obligate pathogens living within host organisms to oligotrophic groundwater ecosystems. Whilst most members of the Betaproteobacteria are heterotrophic, deriving both their carbon and electrons from organocarbon sources, some are photoheterotrophic, deriving energy from light and carbon from organocarbon sources. Other genera are autotrophic, deriving their carbon from bicarbonate or carbon dioxide and their electrons from reduced inorganic ions such as nitrite, ammonium, thiosulfate or sulfide [1] - many of these chemolithoautotrophic Betaproteobacteria are economically important, with roles in maintaining soil pH and in elementary cycling. Other economically ...
Brucella species Bubonic plague Yersinia Pestis Burkholderia infection usually Burkholderia cepacia and other Burkholderia ...
Toxoplasmosis is becoming a global health hazard as it infects 30-50% of the world human population. Clinically, the life-long presence of the parasite in tissues of a majority of infected individuals is usually considered asymptomatic. However, a number of studies show that this 'asymptomatic infection' may also lead to development of other human pathologies. ... The seroprevalence of toxoplasmosis correlated with various disease burden. Statistical associations does not necessarily mean causality. The precautionary principle suggests however that possible role of toxoplasmosis as a triggering factor responsible for development of several clinical entities deserves much more attention and financial support both in everyday medical practice and future clinical research ...
... [1], previously known as Enterobacter aerogenes, is a Gram-negative, oxidase negative, catalase positive, citrate positive, indole negative, rod-shaped bacterium.[2] The bacterium is approximately 1-3 microns in length, and is capable of motility via peritrichous flagella.[3] K. aerogenes is a nosocomial and pathogenic bacterium that causes opportunistic infections including most types of infections. The majority are sensitive to most antibiotics designed for this bacteria class, but this is complicated by their inducible resistance mechanisms, particularly lactamase, which means that they quickly become resistant to standard antibiotics during treatment, requiring a change in antibiotic to avoid worsening of the sepsis. Some of the infections caused by K. aerogenes result from specific antibiotic treatments, venous catheter insertions, and/or surgical procedures. K. aerogenes is generally found in the human gastrointestinal tract and does not generally cause disease in ...
... , also known simply as typhoid, is a bacterial infection due to Salmonella typhi that causes symptoms.[3] Symptoms may vary from mild to severe and usually begin six to thirty days after exposure.[1][2] Often there is a gradual onset of a high fever over several days.[1] Weakness, abdominal pain, constipation, and headaches also commonly occur.[2][6] Diarrhea is uncommon and vomiting is not usually severe.[6] Some people develop a skin rash with rose colored spots.[2] In severe cases there may be confusion.[6] Without treatment, symptoms may last weeks or months.[2] Other people may carry the bacterium without being affected; however, they are still able to spread the disease to others.[4] Typhoid fever is a type of enteric fever along with paratyphoid fever.[3]. The cause is the bacterium Salmonella typhi, also known as Salmonella enterica serotype Typhi, growing in the intestines and blood.[2][6] Typhoid is spread by eating or drinking food or water contaminated with the feces of ...
... (PUD) is a break in the inner lining of the stomach, the first part of the small intestine, or sometimes the lower esophagus.[1][7] An ulcer in the stomach is called a gastric ulcer, while one in the first part of the intestines is a duodenal ulcer.[1] The most common symptoms of a duodenal ulcer are waking at night with upper abdominal pain and upper abdominal pain that improves with eating.[1] With a gastric ulcer, the pain may worsen with eating.[8] The pain is often described as a burning or dull ache.[1] Other symptoms include belching, vomiting, weight loss, or poor appetite.[1] About a third of older people have no symptoms.[1] Complications may include bleeding, perforation, and blockage of the stomach.[2] Bleeding occurs in as many as 15% of cases.[2] Common causes include the bacteria Helicobacter pylori and non-steroidal anti-inflammatory drugs (NSAIDs).[1] Other, less common causes include tobacco smoking, stress due to serious illness, Behcet disease, ...
With a fatality risk approaching 15% within 12 hours of infection, it is crucial to initiate testing as quickly as possible, but not to wait for the results before initiating antibiotic therapy. A small amount of cerebrospinal fluid (CSF) is sent to the laboratory as soon as possible for analysis. The diagnosis is suspected, when Gram-negative diplococci are seen on Gram stain of a centrifuged sample of CSF; sometimes they are located inside white blood cells. The microscopic identification takes around 1-2 hours after specimen arrival in the laboratory.[3] The gold standard of diagnosis is microbiological isolation of N. meningitidis by growth from a sterile body fluid, which could be CSF or blood.[5] Diagnosis is confirmed when the organism has grown, most often on a chocolate agar plate, but also on Thayer-Martin agar. To differentiate any bacterial growth from other species a small amount of a bacterial colony is tested for oxidase, catalase for which all clinically relevant Neisseria show a ...
Plague has a long history as a biological weapon. Historical accounts from ancient China and medieval Europe detail the use of infected animal carcasses, such as cows or horses, and human carcasses, by the Xiongnu/Huns, Mongols, Turks and other groups, to contaminate enemy water supplies. Han Dynasty General Huo Qubing is recorded to have died of such a contamination while engaging in warfare against the Xiongnu. Plague victims were also reported to have been tossed by catapult into cities under siege. In 1347, the Genoese possession of Caffa, a great trade emporium on the Crimean peninsula, came under siege by an army of Mongol warriors of the Golden Horde under the command of Janibeg. After a protracted siege during which the Mongol army was reportedly withering from the disease, they decided to use the infected corpses as a biological weapon. The corpses were catapulted over the city walls, infecting the inhabitants. This event might have led to the transfer of the plague (Black Death) via ...
... , also known simply as paratyphoid, is a bacterial infection caused by one of the three types of Salmonella enterica.[1] Symptoms usually begin 6-30 days after exposure and are the same as those of typhoid fever.[1][3] Often, a gradual onset of a high fever occurs over several days.[1] Weakness, loss of appetite, and headaches also commonly occur.[1] Some people develop a skin rash with rose-colored spots.[2] Without treatment, symptoms may last weeks or months.[1] Other people may carry the bacteria without being affected; however, they are still able to spread the disease to others.[3] Both typhoid and paratyphoid are of similar severity.[3] Paratyphoid and typhoid fever are types of enteric fever.[7] Paratyphoid is caused by the bacterium Salmonella enterica of the serotypes Paratyphi A, Paratyphi B, or Paratyphi C growing in the intestines and blood.[1] They are usually spread by eating or drinking food or water contaminated with the feces of an infected person.[1] They may ...
and Brucella spp. - cause septic spinal arthritis Eikenella corrodens - human bites Pasteurella multocida, bartonella henselae ...
Infection: Acute viral hepatitis Infectious mononucleosis Cytomegalovirus Rubella Brucella infection Hyper active malaria ... "Pathogenesis of Brucella spp". The Open Veterinary Science Journal. 4: 109-118. doi:10.2174/1874318801004010109. "Typhoid & ...
Brucella species survive well in aerosols and resist drying. Brucella and all other remaining biological weapons in the U.S. ... Brucella suis, also referred to as swine brucellosis Wyatt HV (2014). "How did Sir David Bruce forget Zammit and his goats ?" ( ... with the Brucella milk ring test. Cows confirmed to be infected are often killed. In the United States, veterinarians are ... Brucellosis in New Zealand is limited to sheep (B. ovis). The country is free of all other species of Brucella. Dairy herds in ...
Scholz HC, Vergnaud G (2013). "Molecular characterisation of Brucella species". Rev. Sci. Tech. 32 (1): 149-62. doi:10.20506/ ... Brucella. https://web.archive.org/web/20141225151444/http://tandemrepeat.u-psud.fr/ http://minisatellites.u-psud.fr/ http:// ...
Brucella, Giana (21 September 2016). "Forbes Leaves MU off List of 'NJ Top 10 Colleges'". The Outlook. Retrieved 30 August 2020 ...
These included Brucella, gonorrhoea, and syphilis. Adrenaline injections, mercury, various hormone treatments (ovarian extracts ...
It is related to Brucella spp. and was first described by Velasco and others in 1998. It causes diseases in humans only rarely ... Velasco, J; Romero, C; López-Goñi, I; Leiva, J; Díaz, R; Moriyón, I (1998). "Evaluation of the relatedness of Brucella spp. and ... O. intermedium, O. anthropi, and Brucella melitensis can be distinguished on the basis of a multi-primer polymerase chain ... nov., a new species with a closer relationship to Brucella spp". International Journal of Systematic Bacteriology. 48 (3): 759- ...
Jordan, Brucella Wiggins, pp. 195-196. Tichi, p. 340 (note 16). Nettles, Arionne Alyssa. Seymour, pp. 333. Palmer, pp. 106-107 ... Brucella Wiggins (2003). Ida B. Wells, Catherine Impey, and Trans-Atlantic Dimensions of the Nineteenth-Century Anti-Lynching ...
"Characterization of ribonuclease III from Brucella". Gene. 579 (2): 183-192. doi:10.1016/j.gene.2015.12.068. PMID 26778206. " ...
MARR AG, WILSON PW (1954). "The alanine racemase of Brucella abortus". Arch. Biochem. Biophys. 49 (2): 424-33. doi:10.1016/0003 ...
Brucella abortus A13334, 3,401 ORFs Brucella canis ATCC 23365, 3,408 ORFs Brucella melitensis 16M, 3,279 ORFs Brucella microti ... 3,193 ORFs Brucella pinnipedialis B2/94, 3,505 ORFs Brucella suis 1330, 3,408 ORFs Genome data for these and other Brucella ... Brucella species have been found primarily in mammals: Pathogenic Brucella species can cause abortion in female animals by ... Conversely, depriving Brucella of the blue wavelengths dropped its reproductive rate by 90%. Parte, A.C. "Brucella". Muleme.M ...
Describe laboratory tests for identification of Brucella spp.. *Describe how to rule out or refer isolates for Brucella spp. ... This course provides clinical laboratory scientists with information about the laboratory identification of Brucella spp. ...
Three types of the bacteria that cause brucellosis - Brucella abortus, Brucella melitensis and Brucella suis - are designated ... Exposure to most species of Brucella, such as those associated with certain types of animals, could potentially lead to ... Human Neurobrucellosis with Intracerebral Granuloma Caused by a Marine Mammal Brucella spp. ...
Multiporator / Electropo... Making electrocompetent cells: ... Electroporation of cells: Grow ...,Brucella,abortus,biological, ... 4308 915.508 12/2001 Microorganism Brucella abortus Cell type Bacteria, gram negative Molecules injected Plasmid DNA (pBA.sodkn ...
The Brucella abortus (rough LPS Brucella) vaccine, developed for bovine brucellosis and licensed by the USDA Animal Plant ... Since brucellosis threatens the food supply and causes undulant fever, Brucella suis and other Brucella species (B. melitensis ... of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella ... Brucella suis is a bacterium that causes swine brucellosis, a zoonosis that affects pigs. The disease typically causes chronic ...
ABC systems of Brucella melitensis 16M, Brucella abortus 9-941, Brucella canis RM6/66, Brucella suis 1330, and Brucella ovis 63 ... Brucella abortus (cattle), Brucella suis (pigs), Brucella ovis (sheep), Brucella canis (dogs), and Brucella neotomae (desert ... Brucella pinnipedialis (seals) [3], Brucella ceti (dolphins and porpoises) [3], and Brucella microti (voles) [4]. Although ... and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts," International ...
Test Detects Brucella Bacteria in Goats Milk. By Luis Pons. March 24, 2004. Goat milk sold in the United States may soon be ... B. melitensis, one of six known species of Brucella bacteria that induce abortions in animals, mainly infects sheep and goats. ... A test for detecting the bacteria Brucella melitensis in bulk goat milk has been developed by research chemist Louisa Tabatabai ... ARS Home » News & Events » News Articles » Research News » 2004 » Test Detects Brucella Bacteria in Goats Milk ...
bru·cel·lae, or bru·cel·las Any of various aerobic, short, rod-shaped bacteria of the genus Brucella that are pathogenic to ... pl. brucellae (-sĕl′ē) or brucellas Any of various aerobic, short, rod-shaped bacteria of the genus Brucella that are ... brucella. bru·cel·la. noun. pl. bru·cel·lae, or bru·cel·las Any of various aerobic, short, rod-shaped bacteria of the genus ... Brucella f. *A taxonomic genus within the family Brucellaceae "" several gram-negative bacteria that are pathogenic to humans ...
Brucellosis is a systemic infection caused by brucella species. Prosthetic joint infection due to brucella species is rare. We ... An Unusual Case of Brucella abortus Prosthetic Joint Infection.. Walsh J1, Gilleece A2, Fenelon L1, Cogley D3, Schaffer K1. ...
Brucella is a facultative intracellular bacterium responsible for brucellosis. Virulence factors involved in Brucella ... Brucella lipopolysaccharide acts as a virulence factor.. Lapaque N1, Moriyon I, Moreno E, Gorvel JP. ... These properties make lipopolysaccharide an important virulence factor for Brucella survival and replication in the host. ... bacterial outer membrane components have also been described as being implicated in Brucella survival in the host. For example ...
However, positive results by Brucella serology are not necessarily diagnostic of acute infections, as antibodies may persist ...
Brucella serology done by immunocapture assay was significantly raised 1 : 10240. Blood culture isolated Brucella species but ... Synovial cultures isolated Brucella species but blood culture was negative. Brucella serology in the blood was high; it was 1 ... M. I. Khateeb, G. F. Araj, S. A. Majeed, and A. R. Lulu, "Brucella arthritis: a study of 96 cases in Kuwait," Annals of the ... The blood urea and creatinine was raised at 16.8 mmol/L and 140 μmol/L. Synovial fluid cultures were positive for Brucella ...
In the last several decades, much research has been performed to develop safer Brucella vaccines to control the disease mainly ... A history of the development of Brucella vaccines Biomed Res Int. 2013;2013:743509. doi: 10.1155/2013/743509. Epub 2013 Jun 3. ... The aim of this paper is to review and discuss the importance of methodologies used to develop Brucella vaccines in pursuing ... In the last several decades, much research has been performed to develop safer Brucella vaccines to control the disease mainly ...
Complaints of short-term memory loss with no cognitive deficit were common in Gulf War veterans, in whom brucella screening ... Proposed as a cause of chronic fatigue syndrome, we receive regular requests for brucella serology after a patient has visited ... A - Now almost exclusively acquired abroad, brucella is an unlikely cause of his symptoms. An intracellular parasite that is ... due to a reservoir of brucellae in tissues including lymphatics, bones and liver. ...
Thank you for sharing this Journal of Bacteriology article.. NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.. ...
Biotin metabolism - Brucella ovis [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show description , User data ...
Brucella strains microbiology Iron enzyme dependence Manganese enzymes bacteria Zn brucella strain Biometals ... Researchers and professionals studying zoonotic pathogens from the genus Brucella find this book valuable. ... The Role of Zinc in the Biology and Virulence of Brucella Strains ... in the basic biology and virulence of bacterial pathogens from the genus Brucella. The authors explore the biological functions ...
We will assess theeffects of Brucella phages on Brucella replication in macrophages in vitro. The phages will becombined with ... Optimized phage therapy for Brucella infection Investigators. Jutila, MA. Institutions. Montana State University. Start date. ... Brucella can replicateintracellularly, sequestered away from phages. Thus, we will also encapsulate phage inliposomes for ... Brucellosis is a zoonotic infection caused by Brucella species. Two species, B. abortus and B.melitensis, are select agents and ...
Sulfur metabolism - Brucella melitensis M28 [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show description ...
Brucella. Emerging Infectious Diseases. 2008;14(8):1337. doi:10.3201/eid1408.e11408.. ... Bruce established a causal relationship between the disease and an organism later designated Brucella melitensis (from Malta). ... Brucella spp. include animal parasites and pathogens, transmissible to humans through dairy products or contact with infected ... 2008). Brucella. Emerging Infectious Diseases, 14(8), 1337. https://dx.doi.org/10.3201/eid1408.e11408.. ...
Brucella melitensis. Mutation(s): 0 Gene Names: thyA, BMEI0608. EC: ... Find proteins for P67042 (Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)) ... Crystal structure of Thymidylate synthase thyA from Brucella melitensis. Abendroth, J., Edwards, T.E., Seattle Structural ... Crystal structure of Thymidylate synthase thyA from Brucella melitensis. *DOI: 10.2210/pdb3IX6/pdb ...
Distribution of Radioiodinated, Acetone-killed Brucella abortus in the Tissues of Normal and Immune Mice. *D. SULITZEANU1. ... SULITZEANU, D. Distribution of Radioiodinated, Acetone-killed Brucella abortus in the Tissues of Normal and Immune Mice. Nature ... Induced Resistance of Mice to Infection with Brucella abortus 2308 through Vaccination with BCG *A. BEKIERKUNST ...
Brucella abortus, Brucella canis, Brucella cetaceae, Brucella maris , Brucella melitensis, Brucella pinnipediae, Brucella sp ... Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and ... Brucella enters and lives in such different host cells such as epithelial cells are not known. Brucella is generally ... He started anti-brucella therapy, which continued for 1 year and the enhancement and edema receded (Sohn et al. 2003). == ...
This work demonstrates that Brucella lipopolysaccharide (LPS) preparations are a family of related molecules which display ... Heterogeneity of Brucella Abortus Lipopolysaccharides Res Microbiol. 1995 Sep;146(7):569-78. doi: 10.1016/0923-2508(96)80563-8 ... This work demonstrates that Brucella lipopolysaccharide (LPS) preparations are a family of related molecules which display ... Sodium dodecyl sulphate/polyacrylamide gel electrophoresis and Western blotting showed that LPS from Brucella strains displayed ...
The experiments reported describe the direct recovery of Brucella from macrophages infected in vitro and from the spleens of ... The experiments reported describe the direct recovery of Brucella from macrophages infected in vitro and from the spleens of ... However, in situ analysis of Brucella infected macrophages using antibody directed against O-polysaccharide suggested a loss of ... However, in situ analysis of Brucella infected macrophages using antibody directed against O-polysaccharide suggested a loss of ...
Thank you all for the ideas you have shared with me regarding my daughters headache. Just to review, she developed an unrelenting headache on 11/16/13 and up until last week no doctor has been able to find the reason. Her pain spiked last week and she was admitted to the hospital. Some smart pediatrician thought to look in her eyes and saw optic nerve involvement. Opthamologist confirmed papilledema (swelling of optic nerves), MRI (2nd one this month) showed nothing. So they did a lumbar puncture and she had an opening CSF pressure of 48cm. Highest pressure the docs have ever seen ...
... SKU: GL-1002 Retail Price: $24.50. Our LOW Price: $18.33. You Save: $6.17 (25%). Availability: ... Home » Medical Equipment & Supplies » Instruments & Disposables » Urine Reagent Strips, Individual Packs » Brucella Melitensis ...
We present a rare case of brucella endocarditis, forming a vegetation on the mitral valve. The definitive diagnosis has been ... demonstration of the vegetation with the echocardiography and with the production from the multiple blood culture of brucella ...
This assay utilizes antigen from Brucella abortus strain W99. ... The Brucella abortus IgA ELISA Kit is intended for the ... measurement of human IgA antibodies to Brucella abortus. ... Brucella abortus IgA ELISA Kit (CE). Packaging size: 96 Wells ... The Brucella abortus ELISA Kit is intended for the measurement of human IgG, IgA, or IgM antibodies to Brucella abortus. This ... The Brucella abortus IgA ELISA Kit is intended for the measurement of human IgA antibodies to Brucella abortus. This assay ...
Whenever cultivation of Brucella spp. from blood, bone marrow, or other tissues is attempted, it is essential to notify the ... Brucella spp. are concentrated in the genital tract and milk of infected animals. B. melitensis infects primarily goats, sheep ... Laboratory cultures of Brucella spp. confer a high risk of transmission via aerosolization and should be handled with BIosafety ... Brucella spp. enter hosts via ingestion, inhalation, or inoculation through mucous membranes or non-intact skin. The bacteria ...
  • Brucella is the cause of brucellosis, which is a zoonosis transmitted by ingesting contaminated food (such as unpasteurized milk products), direct contact with an infected animal, or inhalation of aerosols. (wikipedia.org)
  • Three types of the bacteria that cause brucellosis - Brucella abortus , Brucella melitensis and Brucella suis - are designated as select agents. (cdc.gov)
  • Brucella suis is a bacterium that causes swine brucellosis, a zoonosis that affects pigs. (wikipedia.org)
  • Since brucellosis threatens the food supply and causes undulant fever, Brucella suis and other Brucella species (B. melitensis, B. abortis, B. ovis, B. canis) are recognized as potential agricultural, civilian, and military bioterrorism agents. (wikipedia.org)
  • The Brucella abortus (rough LPS Brucella) vaccine, developed for bovine brucellosis and licensed by the USDA Animal Plant Health Inspection Service, has shown protection for some swine and is also effective against B. suis infection, but there is currently no approved vaccine for swine brucellosis. (wikipedia.org)
  • Brucella species are the causative agents of brucellosis, the world's most prevalent zoonotic disease, with high occurrences in endemic areas including the Middle East, Asia, Mexico, and the Mediterranean [ 1 ]. (hindawi.com)
  • Brucellosis is a systemic infection caused by brucella species. (nih.gov)
  • Brucella is a facultative intracellular bacterium responsible for brucellosis. (nih.gov)
  • We propose to use Brucella-specific phages, in combination withinnate immune stimulation, as a novel countermeasure for brucellosis. (usda.gov)
  • Canine brucellosis caused by Brucella canis is a neglected zoonosis worldwide and is a leading cause of reproductive failure in dogs, often causing substantial economic losses in breeding kennels. (cabi.org)
  • None of the exposed persons reported having previously had brucellosis or being unintentionally inoculated with Brucella vaccine. (cdc.gov)
  • Editorial Note: Brucellosis, also known as 'undulant fever' or 'Bangs disease,' is a systemic infection caused by Brucella sp. (cdc.gov)
  • brucellosis - an infectious disease caused by Brucella, and transmitted by direct contact with diseased animals or through ingestion of infected meat, milk, or cheese. (thefreedictionary.com)
  • Human brucellosis is most commonly caused by the species Brucella melitensis and is usually acquired through ingestion of unpasteurized dairy products or, less commonly, through ingestion of infected meat or direct occupational contact with animals ( 1 ). (asm.org)
  • Thirty-five cases of brucellosis brought to our laboratory were treated with Brucella antigens prepared from the bacterial bodies by grinding Brucella of the three varieties. (ajtmh.org)
  • In the study we claimed to identify Brucella species from clinical samples of patients with active brucellosis from Van region of Eastern Anatolia and to determine in vitro antimicrobial susceptibilities of these strains to commonly used anti-Brucella agents and a possible new alternative tigecycline. (medsci.org)
  • Brucella is an intracellular bacterium that causes chronic brucellosis in humans and various mammals. (frontiersin.org)
  • Brucella is a Gram-negative intracellular bacterium that causes zoonotic brucellosis in humans and various animals. (frontiersin.org)
  • Brucellosis is a zoonosis, caused by a bacterium from the genus Brucella that have in raw milk and dairy products an important route of transmission for man. (usp.br)
  • Most of the data is based on sero-epidemiological studies although it is generally accepted that only the isolation of Brucella confirms the presence of brucellosis. (au-ibar.org)
  • Brucella endocarditis, despite its high mortality rate with combined medical and surgical treatment, has a low occurrence rate in cases of brucellosis and has been endemic in regions surrounding Turkey. (ebscohost.com)
  • Brucellosis is a major bacterial zoonoses worldwide (Manish et al, 2013) caused by Brucella species. (thefreelibrary.com)
  • Brucellosis is an important zoonosis caused by Brucella spp. (sigmaaldrich.com)
  • Canine brucellosis is an important, incurable reproductive disease of dogs that is caused by Brucella canis. (pavma.org)
  • Objectives Brucellosis is caused by bacteria from the genus Brucella which infect human and domestic animals as well as wildlife. (ilri.org)
  • Antibody response to Brucella ovis outer membrane proteins in ovine brucellosis. (asm.org)
  • Serological and Nucleic Acid Based Detection of Brucellosis in Livestock Species and Molecular Characterization of Brucella melitensis Strains Isolated from Pakistan. (thefreelibrary.com)
  • Brucellosis is a zoonosis of global socio-economic importance caused by Gram negative facultative intracellular bacteria of genus Brucella. (thefreelibrary.com)
  • The IDEXX Brucellosis Serum X2 Ab Test is an enzyme immunoassay for the detection of antibodies against Brucella abortus in individual serum and plasma, and pools of up to 10 individual serum or plasma samples of ruminants. (idexx.com.au)
  • Brucellosis in cattle is a disease caused by Brucella abortus ( B. abortus ), a facultative, intracellular bacterium. (idexx.com.au)
  • The different species of Brucella are genetically very similar, although each has a slightly different host specificity. (wikipedia.org)
  • Hence, the NCBI taxonomy includes most Brucella species under B. melitensis. (wikipedia.org)
  • People may also be infected by inhalation of contaminated dust or aerosols, and as such, the CDC has labeled Brucella species as highly weaponizable. (wikipedia.org)
  • Exposure to most species of Brucella , such as those associated with certain types of animals, could potentially lead to infection. (cdc.gov)
  • In this study, complete inventories of putative functional ABC systems of five Brucella species have been compiled and compared. (hindawi.com)
  • High numbers of ABC systems, particularly nutrient importers, were found in all Brucella species. (hindawi.com)
  • B. ovis , 59) as well as specific differences in the functional ABC systems of the Brucella species. (hindawi.com)
  • Although Brucella are primarily animal pathogens causing infectious abortions in females and orchitis in males [ 5 ], four of the nine species may infect humans ( B. melitensis , B. abortus, B. suis, and occasionally B. canis , in order of disease severity) causing a range of flu-like symptoms including fever, sweats, malaise, and nausea [ 6 ]. (hindawi.com)
  • Due to the nature of the human disease and the ability to be infectious via aerosol, Brucella species have been classified as category B threat agents by the US Centre for Disease Control and Prevention (CDC) [ 7 ]. (hindawi.com)
  • although there is currently little experimental evidence to support this, a few studies have found differences between the Brucella species genomes that may support this hypothesis [ 10 , 15 , 16 ]. (hindawi.com)
  • B. melitensis , one of six known species of Brucella bacteria that induce abortions in animals, mainly infects sheep and goats. (usda.gov)
  • Prosthetic joint infection due to brucella species is rare. (nih.gov)
  • Blood culture isolated Brucella species but synovial fluid culture was negative. (hindawi.com)
  • Essential questions to ask include when and how the diagnosis was made, the treatment length - months of rifampicin and doxycycline are the norm - and the particular species of brucella implicated. (pulsetoday.co.uk)
  • In addition to this, analyses of three ''Brucella'' species have shown that their genomes lack the functional sequences of so-called classical virulence factors, pathogenic islands, and a complete set of genes to mount, types I, II, and II secretion systems. (kenyon.edu)
  • Lipopolysaccharide-deficient mutants of smooth Brucella species (rough mutants) have been shown to arise spontaneously in culture. (frontiersin.org)
  • On the basis of LPS expression, the classical species can be broken into two broad categories: smooth (S-) or rough (R-). The classification between S- and R-strains is based on the observation of Brucella colonies using the oblique light method ( Henry, 1933 ). (frontiersin.org)
  • The O-polysaccharide of Brucella LPS is critical for virulence of the classical Brucella species ( B. melitensis, B. abortus , B. suis ). (frontiersin.org)
  • Brucella species are small, aerobic, Gram-negative coccobacilli that are animal pathogens of worldwide distribution. (renalandurologynews.com)
  • It is the type species of the genus Brucella. (thefreedictionary.com)
  • a similar organism to the other brucellae except that it is inhibited in growth by a 10% concentration of CO 2 , a cultural enhancement for the other species. (thefreedictionary.com)
  • The type species is Brucella melitensis . (thefreedictionary.com)
  • were confirmed as Brucella abortus by PCR using species-specific primers. (usp.br)
  • Mapping the restriction fragments of the Brucella melitensis 16M genome with a new restriction endonuclease, PacI, which cut the DNA into only eight fragments, indicated that this species contains two unique and independent replicons of about 2,100 and 1,150 kb. (asm.org)
  • The Brucella species found in marine mammals differ from those of livestock. (evira.fi)
  • The Brucella ceti species is prevalent in dolphins and whales. (evira.fi)
  • Most of the information about the inter-species interactions between host and Brucella genes is only available in the text of the scientific publications. (frontiersin.org)
  • Next, sentence-level and abstract-level co-occurrence based approaches, as well as sentence-level machine learning based methods, originally designed for extracting intra-species gene interactions, were utilized to extract the interactions among the identified host and Brucella genes. (frontiersin.org)
  • Pathogenic Brucella species adapted to an intracellular lifestyle have two genes involved in riboflavin synthesis, ribH1 and ribH2, which are located on different chromosomes. (omicsonline.org)
  • Genus Brucella is classified into different species based on phenotypic differences and host preferences. (thefreelibrary.com)
  • 1987). However, since 1994, several new Brucella species have been isolated from marine mammals (Foster et al. (thefreelibrary.com)
  • 2008). More recently, several species of Brucella have been isolated from human (Scholz et al. (thefreelibrary.com)
  • Marine Brucella species are zoonotic as evidenced by four documented human cases. (vin.com)
  • Terrestrial Brucella cause significant morbidity in host-adapted species, while non-host adapted species may either become chronically infected or clear the infection, depending in part on the lipopolysaccharide structure of the Brucella . (vin.com)
  • Whole blood was incubated with dichlorodihydrofluorescein diacetate (DCFDA), a marker of reactive oxygen species, and challenged with either Staphylococcus aureus or marine Brucella labeled with propidium iodide. (vin.com)
  • As is the case with terrestrial Brucella species, marine Brucella was phagocytosed primarily by monocytes, with minimal granulocyte response in comparison to S. aureus infection. (vin.com)
  • An Unusual Case of Brucella abortus Prosthetic Joint Infection. (nih.gov)
  • An intracellular parasite that is difficult to eradicate, brucella is characteristically associated with chronic relapsing infection, due to a reservoir of brucellae in tissues including lymphatics, bones and liver. (pulsetoday.co.uk)
  • We hypothesize that Stimulation of the innateimmune system together with phage therapy, with or without liposome delivery, willresult in synergistic and successful protection from Brucella infection. (usda.gov)
  • However, in situ analysis of Brucella infected macrophages using antibody directed against O-polysaccharide suggested a loss of reactivity of Brucella consistent with the appearance of rough organisms, and a potential contribution to infection. (frontiersin.org)
  • Brucella canis infection in dogs from commercial breeding kennels in Brazil. (cabi.org)
  • On May 26-27, 1997, nine persons (a farmer, four veterinary clinicians, and four veterinary students) in Manhattan, Kansas, participated in an attempted vaginal delivery, a cesarean delivery, and a necropsy on a stillborn calf that died because of Brucella abortus infection. (cdc.gov)
  • Brucella - a genus of encapsulated, nonmotile bacteria (family Brucellaceae) causing infection of the genital organs, the mammary gland, and the respiratory and intestinal tracts. (thefreedictionary.com)
  • The identification of host- Brucella interaction is crucial to understand host immunity against Brucella infection and Brucella pathogenesis against host immune responses. (frontiersin.org)
  • Brucella infection can induce A20 expression in macrophages. (sigmaaldrich.com)
  • Brucella melitensis primarily affects the reproductive tract of sheep and goats, and B. melitensis infection is characterized by abortion, retained placenta and, to a lesser extent, impaired fertility. (au-ibar.org)
  • We report a case of Brucella melitensis infection of CIED where, contrary to most authorities recommending removal of device, because of the patient's multiple comorbid conditions and age, an attempt was made to keep the device and place the patient on lifelong prophylaxis treatment. (dovepress.com)
  • No correlation was observed between Brucella infection and pathology. (wur.nl)
  • Co-infection with Rift Valley fever virus, Brucella spp. (ilri.org)
  • Deletion of purE attenuates Brucella melitensis infection in mice. (asm.org)
  • It is possible that there is a link between an infection with Brucella and the outbreak of multiple sclerosis. (rapidtest.com)
  • During an antibiotic therapy or a chronic infection, the detection of Brucella spec. (rapidtest.com)
  • Brucella endocarditis is a rare, mostly ignored and missed clinical infection. (bmj.com)
  • Infection of mononuclear phagocytes occurs through lipid raft-mediated entry or phagocytosis, and the Brucella replicates intracellularly after attenuating the host cell response. (vin.com)
  • Human monocytes and granulocytes both phagocytosed large amounts of Brucella as well as S. aureus , unlike beluga granulocytes which displayed a minimal phagocytic response to Brucella infection. (vin.com)
  • This is a bacterial infection caused by a number of types of Brucella bacteria ( Brucella abortus, B. melitensis, B. suis, and B canis ). (sa.gov.au)
  • Brucella infection is mainly an occupational disease of farm workers, veterinarians and abattoir workers. (sa.gov.au)
  • Brucella suis is a Gram-negative, facultative, intracellular coccobacillus, capable of growing and reproducing inside of host cells, specifically phagocytic cells. (wikipedia.org)
  • Brucella suis is differentiated into five biovars (strains), where biovars 1-3 infect wild boar and domestic pigs, and biovars 1 and 3 may cause severe diseases in humans. (wikipedia.org)
  • Summary: Brucella suis encounters a macrophage, but no oxidative burst occurs. (wikipedia.org)
  • ABC systems of Brucella melitensis 16M, Brucella abortus 9-941, Brucella canis RM6/66, Brucella suis 1330, and Brucella ovis 63/290 were identified and aligned. (hindawi.com)
  • Brucella melitensis (which usually infects sheep and goats), Brucella abortus (cattle), Brucella suis (pigs), Brucella ovis (sheep), Brucella canis (dogs), and Brucella neotomae (desert wood rats). (hindawi.com)
  • 2002). ''Brucella'' ''suis'' was the first pathogenic organism used by the U.S. military as a weapon backing in the 1950s. (kenyon.edu)
  • Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella suis. (thefreedictionary.com)
  • A partial match was found for Brucella suis Please try browsing our store to find what you want. (avivasysbio.com)
  • Rabbit polyclonal antibody raised against full length recombinant Brucella suis vjbR. (abnova.com)
  • Recombinant protein corresponding to full length Brucella suis vjbR. (abnova.com)
  • The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts. (abnova.com)
  • Brucella abortus, Brucella melitensis and Brucella suis ) other than B. canis is also recommended. (scielo.org.za)
  • However, positive results by Brucella serology are not necessarily diagnostic of acute infections, as antibodies may persist for months to years, in some patients, following exposure. (sgh.com.sg)
  • The Brucella abortus IgA ELISA Kit is intended for the measurement of human IgA antibodies to Brucella abortus. (mpbio.com)
  • Probing the surface of bvrR and bvrS transposon mutants with monoclonal antibodies showed all described major outer membrane proteins (Omps) but Omp25, a protein known to be involved in Brucella virulence. (pnas.org)
  • Evaluation of a fluorescence polarization assay for the detection of serum antibodies to Brucella abortus in water buffalo ( Bubalus bubalis ). (springer.com)
  • Using parallel interpretation, RBT and iELISA results showed that 31.7% (95% confidence interval (CI): 26.1, 36.3) of sheep and 34.0% (95% CI: 24.7, 44.3) of goats had antibodies against Brucella in the study area. (mdpi.com)
  • In an infected animal, this follow up testing conducted after 4 weeks normally will show the RSAT-2ME test to be positive following conversion of IgM to IgG antibodies against Brucella. (pavma.org)
  • In this study, cattle from three villages at varying distance from the reserve, were screened for antibodies against Brucella abortus. (ilri.org)
  • The Diagnostic Automation Inc. Brucella IgG Antibody ELISA Test Kit has been designed for the the detection and the quantitative determination of specific IgG antibodies against Brucella in serum and plasma. (rapidtest.com)
  • A binding between the IgA antibodies of the serum and the immobilized Brucella antigen takes place. (rapidtest.com)
  • Agglutination test for Brucella mainly identifies which class of antibodies? (indiabix.com)
  • A preliminary serological study of 366 household dogs in Lagos and Ibadan, southwestern Nigeria, was carried out to determine antibodies due to exposure to Brucella abortus and B. canis, using the rose bengal test (RBT) and the rapid slide agglutination (RSA) test, respectively. (scielo.org.za)
  • A cross-sectional study was conducted in order to detect antibodies for Brucella canis (B. canis ) in dogs from urban Harare and five selected rural communities in Zimbabwe. (scielo.org.za)
  • Hot saline extracts of Brucella ovis were composed of vesicles with outer membrane proteins (OMPs), lipopolysaccharide, and phospholipid as constituents. (asm.org)
  • O objetivo do estudo foi adaptar e avaliar a PCR para detecção de Brucella ovis e comparar os resultados com aqueles obtidos por cultivo microbiológico do sêmen, urina e dos órgãos de carneiros infectados experimentalmente. (scielo.br)
  • Brucella ovis is the main cause of a clinical or subclinical disorder characterized by epididymitis and subsequent decrease in fertility in rams (Manterola et al . (scielo.br)
  • Describe how to rule out or refer isolates for Brucella spp. (cdc.gov)
  • A total of 56 Brucella isolates were enrolled the study and the identification of the isolates were based on conventional methods. (medsci.org)
  • The aim of this study was to evaluate the rate of agreement of two in vitro tests for synergy, E test and the checkerboard method, with various combinations of antibiotics that penetrate the cell wall against Brucella melitensis isolates recovered from blood. (asm.org)
  • Antimicrobial susceptibility testing of the Brucella isolates was performed by two different techniques: E test and the checkerboard method. (asm.org)
  • Eight Peruvian isolates of Brucella spp. (scielo.org.pe)
  • All isolates of Brucella spp. (scielo.org.pe)
  • Conclusion: The conditions of a PCR assay to detect Peruvian isolates of Brucella spp, which has been shown to be very sensitive and specific in the laboratory have been tandardized. (scielo.org.pe)
  • We sequenced the full genome and characterised the genetic diversity of two Brucella melitensis isolates from Malaysia and the Philippines. (biomedcentral.com)
  • There are currently no images for Brucella Antibody (NB110-8009R). (novusbio.com)
  • Be the first to review our Brucella Antibody (Bx85) [DyLight 550] and receive a gift card or discount. (novusbio.com)
  • Antibody response and antigen-specific gamma-interferon profiles of vaccinated and unvaccinated pregnant sheep experimentally infected with Brucella melitensis . (springer.com)
  • The Diagnostic Automation Inc. Brucella IgG antibody test kit is based on the principle of the enzyme immunoassay (EIA). (rapidtest.com)
  • Do you live in Bayamon, Puerto Rico and need a brucella antibody igg test? (personalabs.com)
  • In most cases your brucella antibody igg results will be available to view inside your secure Personalabs patient account within 72 hours or less. (personalabs.com)
  • The Diagnostic Automation Inc. Brucella IgA antibody test kit is based on the principle of the enzyme immunoassay (EIA). (rapidtest.com)
  • In 1887 Dr. Bruce established a causal relationship between the disease and an organism later designated Brucella melitensis (from Malta). (cdc.gov)
  • Immunization of rabbits with concentrated Brucella abortus antigens for 10 weeks led to nearly total loss of serum bactericidal activity for this organism. (jimmunol.org)
  • Researchers and professionals studying zoonotic pathogens from the genus Brucella find this book valuable. (springer.com)
  • Brucella is one of the world's major zoonotic pathogens. (ebscohost.com)
  • 1996), DNA vaccines (Munoz et al, 2004), genetically modified vaccines, vectored vaccines (Ruecker and Guzman, 2012) have been tried with varying degree of success but none showed the results equivalent to the Brucella S19 vaccine (Schurig et al. (thefreelibrary.com)
  • Therefore, hsp-60 can be a good antigenic candidate for engineering subunit vaccine against Brucella , as well as for ELISA test development. (scialert.net)
  • The blood culture positive bacterial isolations were further subjected to classical biotyping and molecular techniques for characterization and found as non-vaccine strains of Brucella melitensis. (thefreelibrary.com)
  • We present a rare case of brucella endocarditis, forming a vegetation on the mitral valve. (ebscohost.com)
  • Succesful management of Brucella melitensis endocarditis with combined medical and surgical approach. (thefreedictionary.com)
  • A patient is reported on with brucella endocarditis and persistent fever. (ebscohost.com)
  • This is the second reported case in the literature of brucella endocarditis with splenic abscess. (ebscohost.com)
  • Brucella Endocarditis with Splenic Abscess: A Report of the First Case Diagnosed in Korea. (ebscohost.com)
  • The most successful treatment outcomes of Brucella endocarditis have been reported with usage of both. (ebscohost.com)
  • In all published studies on Brucella infective endocarditis with a cardiovascular implantable device, the recommendation was for device removal and extended treatment with doxycycline combined with rifampin and/or trimethoprim-sulfamethoxazole, plus parenteral aminoglycosides for the first 3 weeks. (dovepress.com)
  • Brucella abortus endocarditis. (nii.ac.jp)
  • Brucella abortus is a particularly pesky pathogen. (uchospitals.edu)
  • Entry of the facultative intracellular pathogen Brucella into host cells results in the formation of endosomal Brucella -containing vacuoles (eBCVs) that initially traffic along the endocytic pathway. (biologists.org)
  • Monocyte respiratory burst response to Brucella was low, consistent with the proposed mechanism of reduced intracellular response to this pathogen. (vin.com)
  • Antimicrobial susceptibility tests are not yet standardized for brucellae, and routine susceptibility tests cannot be performed in microbiology laboratories. (asm.org)
  • It is concluded that the Bactec FX system is able to detect the vast majority of episodes of Brucella bacteremia within the 1-week incubation protocol instituted in most clinical microbiology laboratories and without the need to perform blind subcultures of negative vials, enabling early diagnosis and saving labor and incubation time and space. (asm.org)
  • Recent studies, however, have demonstrated that automated blood culture systems based on monitoring of CO 2 production have improved sensitivity, compared to manual methods, enabling detection of brucellae in pediatric patients within the customary 1-week incubation protocol followed in most clinical microbiology laboratories ( 6 ). (asm.org)
  • In this section you can learn and practice Microbiology Questions based on "Brucella" and improve your skills in order to face the interview, competitive examination and various entrance test (CAT, GATE, GRE, MAT, Bank Exam, Railway Exam etc.) with full confidence. (indiabix.com)
  • Where can I get Microbiology Brucella questions and answers with explanation? (indiabix.com)
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  • After exposure to Brucella, humans generally have a two- to four-week latency period before exhibiting symptoms, which include acute undulating fever (>90% of all cases), headache, arthralgia (>50%), night sweats, fatigue, and anorexia. (wikipedia.org)
  • Transmission to humans takes place via three recognised channels: (i) the consumption of infected animal products, (ii) direct contact with infected animal birth products, and (iii) the inhalation of aerosolised Brucella . (hindawi.com)
  • Any of various aerobic, short, rod-shaped bacteria of the genus Brucella that are pathogenic to humans and domestic animals. (yourdictionary.com)
  • Although many of the Brucella bacteria prevalent in livestock are also known to be human pathogens, only a few infections in humans caused specifically by Brucella pinnipedialis have been reported in the world. (evira.fi)
  • The brucellae are facultative intracellular parasites of animals and humans causing a disease of worldwide importance. (pnas.org)
  • In addition, because of the low infecting dose, the high infectivity, and the ability to cause prolonged disability in humans and loss of productivity in animal husbandry, brucellae are potential candidates for biological warfare ( 3 ). (asm.org)
  • Brucella lipopolysaccharide acts as a virulence factor. (nih.gov)
  • Virulence factors involved in Brucella replication and Brucella's strategies to circumvent the immune response are under investigation. (nih.gov)
  • These properties make lipopolysaccharide an important virulence factor for Brucella survival and replication in the host. (nih.gov)
  • This book summarises the current knowledge on the role played by biologically relevant transition metals (Fe, Mn, Zn, Ni, Co, Cu and Mg) in the basic biology and virulence of bacterial pathogens from the genus Brucella. (springer.com)
  • So far, only one such system, the two-component regulatory system BvrR/BvrS, has been conclusively implicated in Brucella virulence ( 2 ). (pnas.org)
  • Similar to other facultative intracellular parasites, Brucella organisms survive outside cells, but they must infect and replicate intracellularly in animals to perpetuate. (pnas.org)
  • Brucella melitensis , with a reservoir in sheep, goats, and camels may cause severe, acute disease and disabling complications. (thefreedictionary.com)
  • Surveillance of Brucella melitensis and Brucella abortus from aborted Bengal goats in Bangladesh. (thefreedictionary.com)
  • Other organisms enzootic in fauna in this area were Rickettsia rickettsii, Brucella neotomae, Br. (ajtmh.org)
  • Although these " brucella bombs " have long since been destroyed, the bacterium remains a concern around the world. (uchospitals.edu)
  • M. incognitus is thought to be a mutated form of the Brucella bacterium combined with the Visna virus, from which the mycoplasma is extracted. (wikipedia.org)
  • By sequencing DNA extracted from medieval human remains through shotgun metagenomics, without target-specific capture or amplification, we have obtained a draft genome sequence of an ~700-year-old Brucella melitensis strain. (asm.org)
  • This assay utilizes antigen from Brucella abortus strain W99. (mpbio.com)
  • Moreover, the protein was found to be an immunodominant Brucella antigen and was able to generate strong humoral as well as cellular immunity against Brucella abortus in mice. (omicsonline.org)
  • Brucella antigen is bound on the surface of the microtiter strips. (rapidtest.com)
  • The cheeses, sold under the brand names of Le Campagnard, Gaston, and Pernet Mugnier Christian, are being recalled in France following the detection of the bacteria Brucella in the unpasteurised milk used to make them. (foodsafetynews.com)
  • A PCR assay using primers designed based on the gene that encodes a 31 KDa immunogenic protein from Brucella abortus was used in the detection of Brucella spp. (usp.br)
  • Detection and characterization of Brucella spp. (plos.org)
  • This study aimed at molecular detection and characterization of Brucella spp from Pakistan. (thefreelibrary.com)
  • Although brucellar infections can be ascertained with serological tests and nucleic acid amplification assays, culture detection of circulating Brucella organisms remains a diagnostic cornerstone. (asm.org)
  • In addition to this secretion system, bacterial outer membrane components have also been described as being implicated in Brucella survival in the host. (nih.gov)
  • Although bacterial isolation was used as reference, Brucella spp. (usp.br)
  • Isolation of Brucella pinnipedialis from Grey Seals ( Halichoerus grypus ) in the Baltic Sea. (evira.fi)
  • Because of the fastidious nature of members of the genus Brucella , prolonged incubation of media and performance of blind subcultures of negative blood culture vials have been traditionally recommended to maximize isolation of these organisms ( 4 , 5 ). (asm.org)
  • Two different synergy testing methods, the checkerboard and the E test methods, were used to compare the in vitro efficacies of various antimicrobial combinations against 16 Brucella melitensis strains isolated from blood cultures. (asm.org)
  • A0A1M4L9I0_9RHIZ Uncharacterized protein OS=Brucella sp. (uniprot.org)
  • The SciMiner tool, originally designed for detecting mammalian gene/protein names in text, was extended to identify host and Brucella gene/protein names in the abstracts. (frontiersin.org)
  • In a paper recently published by The Journal of Biological Chemistry , Heaton and her colleagues examined one toxin-antitoxin system from Brucella abortus with a wide variety of scientific techniques, from genetic manipulation and cell culture experiments carried out at the Ricketts Regional Biocontainment Laboratory at Argonne to biophysical analyses of protein structure. (uchospitals.edu)
  • Gram-negative, non-spore-forming, nonmotile coccobacilli which were oxidase and urease positive were tested for agglutination with monospecific anti- Brucella serum (Remel Inc., Lenexa, Kans. (asm.org)
  • The primary aim of this study was to assess the presence of Brucella pinnipedialis in marine mammals living along the Dutch coast and to observe a possible correlation between the presence of B. pinnipedialis and accompanying pathology found in infected animals. (wur.nl)
  • Serological methods like agglutination, complement fixation reaction, Brucella Coombs test and ELISA are good alternatives. (rapidtest.com)
  • The aim of this study was to estimate the diversity and prevalence of both groups of Brucella canis 1 and 2 with and without deletion respectively in different areas of Argentina. (elsevier.es)
  • The overall prevalence of Brucella spp. (wur.nl)
  • An initially negative brucella serology, 2 months ago, is now positive 1 : 10240 (Table 1 ). (hindawi.com)
  • Brucella serology done by immunocapture assay was significantly raised 1 : 10240. (hindawi.com)
  • Proposed as a cause of chronic fatigue syndrome, we receive regular requests for brucella serology after a patient has visited alternative health practitioners. (pulsetoday.co.uk)
  • The definitive diagnosis has been made with clinical suspicion, positive serology, the demonstration of the vegetation with the echocardiography and with the production from the multiple blood culture of brucella melitensis and from the excised valve. (ebscohost.com)
  • 2011). Nowadays, more specific techniques such as Polymerase Chain Reaction (PCR) are used in Brucella spp. (scielo.br)
  • Brucella phage lysate bacterin induces elevated TLRs and cytokines response in murine model. (thefreelibrary.com)
  • most importantly, it is caused by bacteria of the genus Brucella [ 1 ]. (intechopen.com)
  • This work demonstrates that Brucella lipopolysaccharide (LPS) preparations are a family of related molecules which display heterogeneity not only at the level of the O polysaccharide, but also at the core oligosaccharide and the lipid A. Sodium dodecyl sulphate/polyacrylamide gel electrophoresis and Western blotting showed that LPS from Brucella strains displayed molecular weight and antigenic heterogeneity. (nih.gov)
  • Preparations of lipopolysaccharide (LPS) from rough and smooth strains of Brucella abortus were mitogenic for spleen cells of athymic nude mice, C3H/HeAU mice, and the endotoxin-resistant C3H/HeJ mice. (jimmunol.org)
  • In the last several decades, much research has been performed to develop safer Brucella vaccines to control the disease mainly in animals. (nih.gov)
  • The aim of this paper is to review and discuss the importance of methodologies used to develop Brucella vaccines in pursuing this challenge. (nih.gov)
  • In past, inactivated Brucella vaccines have also been tried with limited success (Plommet et al. (thefreelibrary.com)
  • 2017 Market Research Report on Brucella Abortus Vaccines Industry was a professional and depth research report on Brucella Abortus Vaccines industry that you would know the world's major regional market conditions of Brucella Abortus Vaccines industry, the main region including North American, Europe and Asia etc., and the main country including United States ,Germany ,Japan and China etc. (reportsweb.com)
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  • Results show that the seroconversion induced by Brucella abortus RB51 and RB51-SOD vaccines is lower than that by Brucella melitensis Rev-1. (intechopen.com)
  • in a goat flock and the seroconversion of three groups of animals vaccinated with Rev-1 (Brucella melitensis), RB51, and RB51-SOD (Brucella abortus) to estimate the level of protection conferred on susceptible females. (intechopen.com)
  • The studies of Finnish Food Safety Agency Evira have for the first time identified Brucella pinnipedialis bacteria in Baltic grey seals. (evira.fi)
  • Brucella pinnipedialis bacteria have usually been isolated from healthy seals as an incidental finding. (evira.fi)
  • Brucella pinnipedialis has been found in seals in North Atlantic, Northern Ireland and Canadian waters, in the Scottish and German water areas in the North Sea, as well as in the Pacific Ocean off California. (evira.fi)
  • Brucella is a genus of Gram-negative bacteria, named after David Bruce (1855-1931). (wikipedia.org)
  • Effectiveness of Rose Bengal test and fluorescence polarization assay in the diagnosis of Brucella spp. (springer.com)
  • Objetive: To standardize a PCR assay for detecting Brucella spp. (scielo.org.pe)
  • Because brucellae are localized intracellularly, infections with these bacteria should be treated with antibiotics that can penetrate the cell at high concentrations. (asm.org)
  • Some infections with Brucella can cause Brucella Hepatitis. (rapidtest.com)
  • The aim of this study was to determine the seroprevalence of Brucella spp. (intechopen.com)
  • Because these properties relate to the structure of the Brucella outer membrane ( 3-5 ), we reasoned that some of its molecular features should be under the control of BvrR/BvrS. (pnas.org)
  • Brucella organisms have to deal with two very different environments during their life cycle. (pnas.org)
  • Standardization of the technique enabled the differentiation of all Brucella spp. (usp.br)
  • 2002). ==Genome Structure== The genome of ''Brucella'' ''melitensis'' strain 16M is comprised of 3,294,931 bp in two circular chromosomes. (kenyon.edu)