Brucella: A genus of gram-negative, aerobic bacteria that causes BRUCELLOSIS. Its cells are nonmotile coccobacilli and are animal parasites and pathogens. The bacterium is transmissible to humans through contact with infected dairy products or tissue.Brucella abortus: A species of the genus BRUCELLA whose natural hosts are cattle and other bovidae. Abortion and placentitis are frequently produced in the pregnant animal. Other mammals, including humans, may be infected.Brucella melitensis: A species of the genus BRUCELLA whose natural hosts are sheep and goats. Other mammals, including humans, may be infected. In general, these organisms tend to be more virulent for laboratory animals than BRUCELLA ABORTUS and may cause fatal infections.Brucellosis: Infection caused by bacteria of the genus BRUCELLA mainly involving the MONONUCLEAR PHAGOCYTE SYSTEM. This condition is characterized by fever, weakness, malaise, and weight loss.Brucella Vaccine: A bacterial vaccine for the prevention of brucellosis in man and animal. Brucella abortus vaccine is used for the immunization of cattle, sheep, and goats.Brucella ovis: A species of the genus BRUCELLA which are pathogenic to SHEEP.Brucella canis: A species of gram-negative bacteria infecting DOGS, the natural hosts, and causing canine BRUCELLOSIS. It can also cause a mild infection in humans.Brucellosis, Bovine: A disease of cattle caused by bacteria of the genus BRUCELLA leading to abortion in late pregnancy. BRUCELLA ABORTUS is the primary infective agent.Pinnipedia: The suborder of aquatic CARNIVORA comprising the WALRUSES; FUR SEALS; SEA LIONS; and EARLESS SEALS. They have fusiform bodies with very short tails and are found on all sea coasts. The offspring are born on land.Ochrobactrum anthropi: A species of gram-negative, obligately aerobic rods. Motility occurs by peritrichous flagella. (From Bergey's Manual of Determinative Bacteriology, 9th ed)Cetacea: An order of wholly aquatic MAMMALS occurring in all the OCEANS and adjoining seas of the world, as well as in certain river systems. They feed generally on FISHES, cephalopods, and crustaceans. Most are gregarious and most have a relatively long period of parental care and maturation. Included are DOLPHINS; PORPOISES; and WHALES. (From Walker's Mammals of the World, 5th ed, pp969-70)Antibodies, Bacterial: Immunoglobulins produced in a response to BACTERIAL ANTIGENS.Agglutination Tests: Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (From Stedman, 26th ed)Sheep Diseases: Diseases of domestic and mountain sheep of the genus Ovis.Antigens, Bacterial: Substances elaborated by bacteria that have antigenic activity.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Epididymitis: Inflammation of the EPIDIDYMIS. Its clinical features include enlarged epididymis, a swollen SCROTUM; PAIN; PYURIA; and FEVER. It is usually related to infections in the URINARY TRACT, which likely spread to the EPIDIDYMIS through either the VAS DEFERENS or the lymphatics of the SPERMATIC CORD.Abortion, Veterinary: Premature expulsion of the FETUS in animals.Bacterial Proteins: Proteins found in any species of bacterium.Rose Bengal: A bright bluish pink compound that has been used as a dye, biological stain, and diagnostic aid.Virulence: The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.Erythritol: A four-carbon sugar that is found in algae, fungi, and lichens. It is twice as sweet as sucrose and can be used as a coronary vasodilator.Complement Fixation Tests: Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Goats: Any of numerous agile, hollow-horned RUMINANTS of the genus Capra, in the family Bovidae, closely related to the SHEEP.Bacterial Outer Membrane Proteins: Proteins isolated from the outer membrane of Gram-negative bacteria.Mice, Inbred BALB CMacrophages: The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)Spleen: An encapsulated lymphatic organ through which venous blood filters.Virulence Factors: Those components of an organism that determine its capacity to cause disease but are not required for its viability per se. Two classes have been characterized: TOXINS, BIOLOGICAL and surface adhesion molecules that effect the ability of the microorganism to invade and colonize a host. (From Davis et al., Microbiology, 4th ed. p486)Lipopolysaccharides: Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed)Bacterial Typing Techniques: Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping.Genome, Bacterial: The genetic complement of a BACTERIA as represented in its DNA.Bison: A genus of the family Bovidae having two species: B. bison and B. bonasus. This concept is differentiated from BUFFALOES, which refers to Bubalus arnee and Syncerus caffer.Goat Diseases: Diseases of the domestic or wild goat of the genus Capra.Sheep: Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Coombs Test: A test to detect non-agglutinating ANTIBODIES against ERYTHROCYTES by use of anti-antibodies (the Coombs' reagent.) The direct test is applied to freshly drawn blood to detect antibody bound to circulating red cells. The indirect test is applied to serum to detect the presence of antibodies that can bind to red blood cells.Minisatellite Repeats: Tandem arrays of moderately repetitive, short (10-60 bases) DNA sequences which are found dispersed throughout the GENOME, at the ends of chromosomes (TELOMERES), and clustered near telomeres. Their degree of repetition is two to several hundred at each locus. Loci number in the thousands but each locus shows a distinctive repeat unit.Bacterial Vaccines: Suspensions of attenuated or killed bacteria administered for the prevention or treatment of infectious bacterial disease.Porpoises: Mammals of the family Phocoenidae comprising four genera found in the North Pacific Ocean and both sides of the North Atlantic Ocean and in various other seas. They differ from DOLPHINS in that porpoises have a blunt snout and a rather stocky body while dolphins have a beak-like snout and a slender, streamlined body. They usually travel in small groups. (From Walker's Mammals of the World, 5th ed, pp1003-4)Genes, Bacterial: The functional hereditary units of BACTERIA.Pyonephrosis: Distention of KIDNEY with the presence of PUS and suppurative destruction of the renal parenchyma. It is often associated with renal obstruction and can lead to total or nearly total loss of renal function.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Foot Bones: The TARSAL BONES; METATARSAL BONES; and PHALANGES OF TOES. The tarsal bones consists of seven bones: CALCANEUS; TALUS; cuboid; navicular; internal; middle; and external cuneiform bones. The five metatarsal bones are numbered one through five, running medial to lateral. There are 14 phalanges in each foot, the great toe has two while the other toes have three each.Erdheim-Chester Disease: A rare form of non-Langerhans-cell histiocytosis (HISTIOCYTOSIS, NON-LANGERHANS-CELL) with onset in middle age. The systemic disease is characterized by infiltration of lipid-laden macrophages, multinucleated giant cells, an inflammatory infiltrate of lymphocytes and histiocytes in the bone marrow, and a generalized sclerosis of the long bones.Malta: An independent state consisting of three islands in the Mediterranean Sea, south of Sicily. Its capital is Valetta. The major island is Malta, the two smaller islands are Comino and Gozo. It was a Phoenician and Carthaginian colony, captured by the Romans in 218 B.C. It was overrun by Saracens in 870, taken by the Normans in 1090, and subsequently held by the French and later the British who allotted them a dominion government in 1921. It became a crown colony in 1933, achieving independence in 1964. The name possibly comes from a pre-Indoeuropean root mel, high, referring to its rocks, but a more picturesque origin derives the name from the Greek melitta or melissa, honey, with reference to its early fame for its honey production. (From Webster's New Geographical Dictionary, 1988, p719 & Room, Brewer's Dictionary of Names, 1992, p330)United States Department of Agriculture: A cabinet department in the Executive Branch of the United States Government concerned with improving and maintaining farm income and developing and expanding markets for agricultural products. Through inspection and grading services it safeguards and insures standards of quality in food supply and production.Biotin: A water-soluble, enzyme co-factor present in minute amounts in every living cell. It occurs mainly bound to proteins or polypeptides and is abundant in liver, kidney, pancreas, yeast, and milk.Pyruvate Carboxylase: A biotin-dependent enzyme belonging to the ligase family that catalyzes the addition of CARBON DIOXIDE to pyruvate. It is occurs in both plants and animals. Deficiency of this enzyme causes severe psychomotor retardation and ACIDOSIS, LACTIC in infants. EC 6.4.1.1.Pimelic Acids: A group of compounds that are derivatives of heptanedioic acid with the general formula R-C7H11O4.Carbon-Nitrogen Ligases: Enzymes that catalyze the joining of two molecules by the formation of a carbon-nitrogen bond. EC 6.3.Sulfur: An element that is a member of the chalcogen family. It has an atomic symbol S, atomic number 16, and atomic weight [32.059; 32.076]. It is found in the amino acids cysteine and methionine.Sulfur Compounds: Inorganic or organic compounds that contain sulfur as an integral part of the molecule.Endocarditis, Bacterial: Inflammation of the ENDOCARDIUM caused by BACTERIA that entered the bloodstream. The strains of bacteria vary with predisposing factors, such as CONGENITAL HEART DEFECTS; HEART VALVE DISEASES; HEART VALVE PROSTHESIS IMPLANTATION; or intravenous drug use.Prosthesis-Related Infections: Infections resulting from the implantation of prosthetic devices. The infections may be acquired from intraoperative contamination (early) or hematogenously acquired from other sites (late).Joint Prosthesis: Prostheses used to partially or totally replace a human or animal joint. (from UMDNS, 1999)PubMed: A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.Fluorodeoxyglucose F18: The compound is given by intravenous injection to do POSITRON-EMISSION TOMOGRAPHY for the assessment of cerebral and myocardial glucose metabolism in various physiological or pathological states including stroke and myocardial ischemia. It is also employed for the detection of malignant tumors including those of the brain, liver, and thyroid gland. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1162)Positron-Emission Tomography: An imaging technique using compounds labelled with short-lived positron-emitting radionuclides (such as carbon-11, nitrogen-13, oxygen-15 and fluorine-18) to measure cell metabolism. It has been useful in study of soft tissues such as CANCER; CARDIOVASCULAR SYSTEM; and brain. SINGLE-PHOTON EMISSION-COMPUTED TOMOGRAPHY is closely related to positron emission tomography, but uses isotopes with longer half-lives and resolution is lower.Splenic DiseasesBrucella suis: A species of gram-negative bacteria, primarily infecting SWINE, but it can also infect humans, DOGS, and HARES.Riboflavin Synthase: An enzyme that catalyzes the formation of riboflavin from two molecules of 6,7-dimethyl-8-ribityllumazine, utilizing a four-carbon fragment from one molecule which is transferred to the second molecule. EC 2.5.1.9.Riboflavin: Nutritional factor found in milk, eggs, malted barley, liver, kidney, heart, and leafy vegetables. The richest natural source is yeast. It occurs in the free form only in the retina of the eye, in whey, and in urine; its principal forms in tissues and cells are as FLAVIN MONONUCLEOTIDE and FLAVIN-ADENINE DINUCLEOTIDE.Pteridines: Compounds based on pyrazino[2,3-d]pyrimidine which is a pyrimidine fused to a pyrazine, containing four NITROGEN atoms.Veterinary Service, Military: A corps of the armed services concerned with animal medicine, the chief interest of which is the care of government-owned working dogs (as in the military police units), working horses (as in state funerals), and working military dolphins (as in undersea exploration and other activities). In the United States Army Veterinary Corps animal medicine overlaps and interconnects with biomedical research using laboratory research animals. A related activity is laboratory animal care. The Corps provides limited care for privately owned animals of military personnel through non-appropriated funds. Military service veterinarians in the United States Army must be graduates of accredited veterinary schools and must have a state license. (Telephone communication with Lt. Col. William Inskeep II, U.S. Army Veterinary Corps, October 4, 1994)Prions: Small proteinaceous infectious particles which resist inactivation by procedures that modify NUCLEIC ACIDS and contain an abnormal isoform of a cellular protein which is a major and necessary component. The abnormal (scrapie) isoform is PrPSc (PRPSC PROTEINS) and the cellular isoform PrPC (PRPC PROTEINS). The primary amino acid sequence of the two isoforms is identical. Human diseases caused by prions include CREUTZFELDT-JAKOB SYNDROME; GERSTMANN-STRAUSSLER SYNDROME; and INSOMNIA, FATAL FAMILIAL.PrPC Proteins: Normal cellular isoform of prion proteins (PRIONS) encoded by a chromosomal gene and found in normal and scrapie-infected brain tissue, and other normal tissue. PrPC are protease-sensitive proteins whose function is unknown. Posttranslational modification of PrPC into PrPSC leads to infectivity.Prion Diseases: A group of genetic, infectious, or sporadic degenerative human and animal nervous system disorders associated with abnormal PRIONS. These diseases are characterized by conversion of the normal prion protein to an abnormal configuration via a post-translational process. In humans, these conditions generally feature DEMENTIA; ATAXIA; and a fatal outcome. Pathologic features include a spongiform encephalopathy without evidence of inflammation. The older literature occasionally refers to these as unconventional SLOW VIRUS DISEASES. (From Proc Natl Acad Sci USA 1998 Nov 10;95(23):13363-83)PrPSc Proteins: Abnormal isoform of prion proteins (PRIONS) resulting from a posttranslational modification of the cellular prion protein (PRPC PROTEINS). PrPSc are disease-specific proteins seen in certain human and animal neurodegenerative diseases (PRION DISEASES).Nigeria: A republic in western Africa, south of NIGER between BENIN and CAMEROON. Its capital is Abuja.Microbial Sensitivity Tests: Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).Anti-Bacterial Agents: Substances that reduce the growth or reproduction of BACTERIA.Endocarditis: Inflammation of the inner lining of the heart (ENDOCARDIUM), the continuous membrane lining the four chambers and HEART VALVES. It is often caused by microorganisms including bacteria, viruses, fungi, and rickettsiae. Left untreated, endocarditis can damage heart valves and become life-threatening.

Genomic fingerprinting and development of a dendrogram for Brucella spp. isolated from seals, porpoises, and dolphins. (1/532)

Genomic DNA from reference strains and biovars of the genus Brucella was analyzed using pulsed-field gel electrophoresis (PFGE). Fingerprints were compared to estimate genetic relatedness among the strains and to obtain information on evolutionary relationships. Electrophoresis of DNA digested with the restriction endonuclease XbaI produced fragment profiles for the reference type strains that distinguished these strains to the level of species. Included in this study were strains isolated from marine mammals. The PFGE profiles from these strains were compared with those obtained from the reference strains and biovars. Isolates from dolphins had similar profiles that were distinct from profiles of Brucella isolates from seals and porpoises. Distance matrix analyses were used to produce a dendrogram. Biovars of B. abortus were clustered together in the dendrogram; similar clusters were shown for biovars of B. melitensis and for biovars of B. suis. Brucella ovis, B. canis, and B. neotomae differed from each other and from B. abortus, B. melitensis, and B. suis. The relationship between B. abortus strain RB51 and other Brucella biovars was compared because this strain has replaced B. abortus strain 19 for use as a live vaccine in cattle and possibly in bison and elk. These results support the current taxonomy of Brucella species and the designation of an additional genomic group(s) of Brucella. The PFGE analysis in conjunction with distance matrix analysis was a useful tool for calculating genetic relatedness among the Brucella species.  (+info)

Brucella outer membrane lipoproteins share antigenic determinants with bacteria of the family Rhizobiaceae. (2/532)

Brucellae have been reported to be phylogenetically related to bacteria of the family Rhizobiaceae. In the present study, we used a panel of monoclonal antibodies (MAbs) to Brucella outer membrane proteins (OMPs) to determine the presence of common OMP epitopes in some representative bacteria of this family, i.e., Ochrobactrum anthropi, Phyllobacterium rubiacearum, Rhizobium leguminosarum, and Agrobacterium tumefaciens, and also in bacteria reported to serologically cross-react with brucella, i.e., Yersinia enterocolitica O:9, Escherichia coli O:157, and Salmonella urbana. In particular, most MAbs to the Brucella outer membrane lipoproteins Omp10, Omp16, and Omp19 cross-reacted with O. anthropi and P. rubiacearum, which are actually the closest relatives of brucellae. Some of them also cross-reacted, but to a lower extent, with R. leguminosarum and A. tumefaciens. The putative Omp16 and Omp19 homologs in these bacteria showed the same apparent molecular masses as their Brucella counterparts. None of the antilipoprotein MAbs cross-reacted with Y. enterocolitica O:9, E. coli O:157, or S. urbana.  (+info)

Early acidification of phagosomes containing Brucella suis is essential for intracellular survival in murine macrophages. (3/532)

Brucella suis is a facultative intracellular pathogen of mammals, residing in macrophage vacuoles. In this work, we studied the phagosomal environment of these bacteria in order to better understand the mechanisms allowing survival and multiplication of B. suis. Intraphagosomal pH in murine J774 cells was determined by measuring the fluorescence intensity of opsonized, carboxyfluorescein-rhodamine- and Oregon Green 488-rhodamine-labeled bacteria. Compartments containing live B. suis acidified to a pH of about 4.0 to 4.5 within 60 min. Acidification of B. suis-containing phagosomes in the early phase of infection was abolished by treatment of host cells with 100 nM bafilomycin A(1), a specific inhibitor of vacuolar proton-ATPases. This neutralization at 1 h postinfection resulted in a 2- to 34-fold reduction of opsonized and nonopsonized viable intracellular bacteria at 4 and 6 h postinfection, respectively. Ammonium chloride and monensin, other pH-neutralizing reagents, led to comparable loss of intracellular viability. Addition of ammonium chloride at 7 h after the beginning of infection, however, did not affect intracellular multiplication of B. suis, in contrast to treatment at 1 h postinfection, where bacteria were completely eradicated within 48 h. Thus, we conclude that phagosomes with B. suis acidify rapidly after infection, and that this early acidification is essential for replication of the bacteria within the macrophage.  (+info)

Improved method for purification of bacterial DNA from bovine milk for detection of Brucella spp. by PCR. (4/532)

Different methods of extraction of bacterial DNA from bovine milk to improve the direct detection of Brucella by PCR were evaluated. We found that the use of a lysis buffer with high concentrations of Tris, EDTA, and NaCl, high concentrations of sodium dodecyl sulfate and proteinase K, and high temperatures of incubation was necessary for the efficient extraction of Brucella DNA. The limit of detection by PCR was 5 to 50 Brucella CFU/ml of milk.  (+info)

Outer membrane proteins Omp10, Omp16, and Omp19 of Brucella spp. are lipoproteins. (5/532)

The deduced sequences of the Omp10, Omp16, and Omp19 outer membrane proteins of Brucella spp. contain a potential bacterial lipoprotein processing sequence. After extraction with Triton X-114, these three proteins partitioned into the detergent phase. Processing of the three proteins is inhibited by globomycin, a specific inhibitor of lipoprotein signal peptidase. The three proteins were radioimmunoprecipitated from [(3)H]palmitic acid-labeled Brucella abortus lysates with monoclonal antibodies. These results demonstrate that Omp10, Omp16, and Omp19 are lipoproteins.  (+info)

Detection of antibodies to Brucella cytoplasmic proteins in the cerebrospinal fluid of patients with neurobrucellosis. (6/532)

The diagnosis of human neurobrucellosis usually relies on the detection of antibodies to Brucella lipopolysaccharide (LPS) in cerebrospinal fluid (CSF) by agglutination tests or enzyme-linked immunosorbent assay (ELISA). Here we describe the detection of immunoglobulin G (IgG) to cytoplasmic proteins (CP) of Brucella spp. by ELISA and Western blotting in seven CSF samples from five patients with neurobrucellosis. While IgG to CP (titers of 200 to 12, 800) and IgG to LPS (800 to 6,400) were found in the CSF of these patients, these antibodies were not detected in CSF samples from two patients who had systemic brucellosis without neurological involvement. The latter, however, had serum IgG and IgM to both LPS and CP. No reactivity to these antigens was found in CSF samples from 14 and 20 patients suffering from nonbrucellar meningitis and noninfectious diseases, respectively. These findings suggest that, in addition to its usefulness in the serological diagnosis of human systemic brucellosis, the ELISA with CP antigen can be used for the specific diagnosis of human neurobrucellosis.  (+info)

The 18-kDa cytoplasmic protein of Brucella species --an antigen useful for diagnosis--is a lumazine synthase. (7/532)

Previous studies have shown that the detection of antibodies to an 18-kDa cytoplasmic protein of Brucella spp. is useful for the diagnosis of human and animal brucellosis. This protein has now been expressed in recombinant form in Escherichia coli. The recombinant protein is soluble only under reducing conditions, but alkylation with iodoacetamide renders it soluble in non-reducing media. As shown by gel exclusion chromatography, this soluble form arranges in pentamers of 90 kDa. The reactivity of human and animal sera against the recombinant protein was similar to that found with the native protein present in brucella cytoplasmic fraction, suggesting that the recombinant protein is correctly folded. The protein has low but significant homology (30%) with lumazine synthases involved in bacterial riboflavin biosynthesis, which also arrange as pentamers. Biological tests on the crude extract of the recombinant bacteria and on the purified recombinant protein showed that the biological activity of the Brucella spp. 18-kDa protein is that of lumazine synthase. Preliminary crystallographic analysis showed that the Brucella spp. lumazine synthase arranges in icosahedric capsids similar to those formed by the lumazine synthases of other bacteria. The high immunogenicity of this protein, potentially useful for the design of acellular vaccines, could be explained by this polymeric arrangement.  (+info)

Competitive enzyme immunoassay for diagnosis of human brucellosis. (8/532)

The methods commonly used for human brucellosis serological testing are agglutination tests and the complement fixation test (CFT). Among the newer serological tests, primary binding assays were developed to improve sensitivity and specificity. The competitive enzyme immunoassay (CELISA) for the detection of serum antibody to Brucella is a multispecies assay which appears to be capable of differentiating vaccinal and cross-reacting antibodies from antibodies elicited by field infection in cattle. The competing monoclonal antibody used in this assay is specific for a common epitope of smooth lipopolysaccharide (S-LPS). In this study, we compared the CELISA to the classical tests for the diagnosis of human brucellosis. The CELISA cutoff value was determined to calculate its diagnostic specificity and sensitivity. A survey was performed with 911 sera. Of the sera, 341 were from an asymptomatic population that tested negative with conventional serological tests (screening and confirmatory). Based on these samples, the CELISA specificities were determined to be 99.7 and 100% with cutoff values of 28 and 30% inhibition (%I), respectively. In a further study with 393 additional sera from an asymptomatic population found negative by the conventional screening tests, the CELISA specificities were calculated to be 96.5 and 98.8% with cutoff values of 28 and 30%I. The CELISA sensitivities were determined to be 98.3 and 94.8% with cutoff values of 28 and 30%I, respectively, for sera from 116 individuals found positive by the classical tests. For the 51 culture-positive patients, CELISA was positive for 100%, the CFT was positive for 92%, and the standard tube agglutination test (TAT) was positive for 100%. The CELISA specificity was 100% for 31 sera from patients found negative by conventional serological tests but with brucellosis-like symptoms. The CELISA is fairly rapid to perform, somewhat faster than TAT, and cross-reacts less with other antigens (or antibodies) than the conventional tests. Further, the CELISA is simpler to perform that the CFT and may readily be standardized by the use of purified S-LPS antigen and monoclonal antibody for competition.  (+info)

*Brucella

... abortus A13334, 3,401 ORFs Brucella canis ATCC 23365, 3,408 ORFs Brucella melitensis 16M, 3,279 ORFs Brucella microti ... 3,193 ORFs Brucella pinnipedialis B2/94, 3,505 ORFs Brucella suis 1330, 3,408 ORFs Genome data for these and other Brucella ... Brucella species have been found primarily in mammals: Pathogenic Brucella species can cause abortion in female animals by ... Conversely, depriving Brucella of the blue wavelengths dropped its reproductive rate by 90%. Parte, A.C. "Brucella". Muleme.M ...

*Brucella papionis

... is a Gram-negative, non-spore-forming and non-motile bacteria from the family of Brucella which has been ... Parte, A.C. "Brucella". www.bacterio.net. "Brucella papionis". www.uniprot.org. Parker, Charles Thomas; Garrity, George M. " ... "Nomenclature Abstract for Brucella papionis Whatmore et al. 2014.". The NamesforLife Abstracts. doi:10.1601/nm.26142. Whatmore ... "Brucella papionis sp. nov., isolated from baboons (Papio spp.)". International Journal of Systematic and Evolutionary ...

*Brucella vulpis

... is a Gram-negative, non-spore-forming and non-motile bacteria from the family of Brucella which has been ... "Brucella vulpis". www.uniprot.org. Parker, Charles Thomas; Garrity, George M. "Nomenclature Abstract for Brucella vulpis Scholz ... "Brucella vulpis sp. nov., a novel Brucella species isolated from mandibular lymph nodes of red foxes (Vulpes vulpes) in Austria ... isolated from the mandibular lymph nodes of foxes (Vulpes vulpes). Parte, A.C. "Brucella". www.bacterio.net. " ...

*Brucella inopinata

... is a Gram-negative, nonmotile, nonspore-forming coccoid bacterium, first isolated from a breast implant ... "Taxonomy of Brucella." Open Veterinary Science Journal 4.1 (2010): 85-101. Fischer, Dominik; Lorenz, Nadja; Heuser, Wenke; ... LPSN Brucella inopinata at the Encyclopedia of Life. ... "Brucella inopinata sp. nov., isolated from a breast implant ... "Serological Diagnosis of Brucella Infections in Odontocetes". Clinical and Vaccine Immunology. 16 (6): 906-915. doi:10.1128/CVI ...

*Brucella ceti

and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts". International ... Brucella ceti is a Gram-negative species of bacteria. It causes infections and related diseases in cetaceans. Foster, G.; ... Nymo, Ingebjørg H; Tryland, Morten; Godfroid, Jacques (2011). "A review of Brucella infection in marine mammals, with special ... LPSN Brucella ceti at the Encyclopedia of Life NOAA informative brochure. ...

*Brucella pinnipedialis

and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts". International ... link) Ocampo-Sosa, Alain A; García-Lobo, Juan M (2008). "Demonstration of IS711 transposition in Brucella ovis and Brucella ... "The genome sequence of Brucella pinnipedialis B2/94 sheds light on the evolutionary history of the genus Brucella". BMC ... Brucella pinnipedialis is a species of bacteria. It causes infections and related diseases primarily in pinnipeds and cetaceans ...

*Brucella microti

"Isolation of Brucella microti from soil." Emerging infectious diseases 14.8 (2008): 1316. LPSN Brucella microti at the ... Brucella microti is a species of bacteria first isolated from the common vole, Microtus arvalis. Its genome has been sequenced ... Audic S, Lescot M, Claverie JM, Scholz HC (2009). "Brucella microti: the genome sequence of an emerging pathogen". BMC Genomics ... "Brucella microti sp. nov., isolated from the common vole Microtus arvalis". International Journal of Systematic and ...

*Brucella abortus

... enters phagocytes that invade human and animal defenses which in turn, cause chronic disease in the host. The ... Brucella abortus is a Gram-negative proteobacterium in the family Brucellaceae, and is one of the causative agents of ... Dorneles, EM; Sriranganathan, N; Lage, AP (8 July 2015). "Recent advances in Brucella abortus vaccines". Veterinary research. ...

*Brucella sRNA

sRNA BM-sr0117 from Brucella melitensis was identified and shown to be bound to and cleaved by Bm-RNase III. AbcR and AbcR2 ( ... In Brucella melitensis eight novel sRNA genes were identified using bioinformatic and experimental approach. One of them ... They are often bound to the chaperone protein Hfq, which allows them to interact with mRNA(s). In Brucella suis 1330 RNA ... Dong, Hao; Peng, Xiaowei; Wang, Ning; Wu, Qingmin (2014-05-01). "Identification of novel sRNAs in Brucella abortus 2308". FEMS ...

*Brucella melitensis

... genomes and related information at PATRIC, a Bioinformatics Resource Center funded by NIAID Brucella ... Brucella melitensis is a Gram-negative coccobacillus bacterium from the Brucellaceae family. The bacterium causes ovine ... Brucella melitensis, reviewed and published by Wikivet at http://en.wikivet.net/Brucella_melitensis, accessed 2011-08-24. ... brucellosis, along with Brucella ovis. It can infect sheep, cattle, and sometimes humans, and it can be transmitted by the ...

*Brucella canis

... is a Gram-negative proteobacterium in the family Brucellaceae that causes brucellosis in dogs and other canids. ... Brucella canis genomes and related information at PATRIC, a Bioinformatics Resource Center funded by NIAID. ... Morisset R, Spink WW (November 1969). "Epidemic canine brucellosis due to a new species, brucella canis". The Lancet. 2 (7628 ... "Investigation of the spread of Brucella canis via the U.S. interstate dog trade". International Journal of Infectious Diseases ...

*Brucella ovis

... is a Gram-negative coccobacillus from the Brucellaceae family. Along with Brucella melitensis, it is responsible ... Brucella ovis, reviewed and published by Wikivet at http://en.wikivet.net/Brucella_ovis, accessed 24/08/2011.. ...

*Brucella agar

... is a form of agar used to culture species of Brucella and campylobacter jejuni Mangels JI, Douglas BP (October ... 1989). "Comparison of four commercial brucella agar media for growth of anaerobic organisms". J. Clin. Microbiol. 27 (10): 2268 ...

*David Bruce (microbiologist)

Brucella melitensis is the cause of undulant fever in man and of abortion in goats. It is usually transmitted by goat's milk. ... Brucella is the genus and Brucellaceae is the family of the bacteria which was named after him, in recognition of his ... later renamed Brucella melitensis). Themistocles Zammit, one of the members of the commission, discovered the reason behind ... http://www.royalsoced.org.uk/cms/files/fellows/biographical_index/fells_indexp1.pdf Corbel, M.J.; Banai, M. (2015). "Brucella ...

*International Committee on Systematics of Prokaryotes

Genus Brucella; Burkholderia, Ralstonia and related organisms; Campylobacter and related bacteria; Clostridia and Clostridium- ...

*Macrophage

Brucella spp. can remain latent in a macrophage via inhibition of phagosome-lysosome fusion; causes brucellosis (undulant fever ...

*Septic arthritis

and Brucella spp. - cause septic spinal arthritis Eikenella corrodens - human bites Pasteurella multocida, bartonella henselae ...

*Hepatosplenomegaly

Infection: Acute viral hepatitis Infectious mononucleosis Cytomegalovirus Rubella Brucella infection Hyper active malaria ... "Pathogenesis of Brucella spp". The Open Veterinary Science Journal. 4: 109-118. "Typhoid & Paratyphoid Fever - Chapter 3 - 2016 ...

*Brucellosis

Brucella species survive well in aerosols and resist drying. Brucella and all other remaining biological weapons in the U.S. ... The growth of brucellae is extremely slow (they can take up to two months to grow) and the culture poses a risk to laboratory ... Dairy herds in the USA to be certified brucellosis-free are tested at least once a year with the Brucella milk ring test. Cows ... Currently, all cattle of ages 3-8 months is required to be given the Brucella abortus strain 19 vaccine. Australia is free of ...

*Multiple loci VNTR analysis

Scholz HC, Vergnaud G (2013). "Molecular characterisation of Brucella species". Rev Sci Tech. 32 (1): 149-62. doi:10.20506/rst. ... Brucella. http://tandemrepeat.u-psud.fr/ http://minisatellites.u-psud.fr/ http://mlva.u-psud.fr/ http://www.mlva.eu/ http://www ...

*Intermittent hydrarthrosis

These included Brucella, gonorrhoea, and syphilis. Adrenaline injections, mercury, various hormone treatments (ovarian extracts ...

*Ribonuclease III

"Characterization of ribonuclease III from Brucella". Gene. 579 (2): 183-192. doi:10.1016/j.gene.2015.12.068. PMID 26778206. " ...

*Alanine racemase

MARR AG, WILSON PW (1954). "The alanine racemase of Brucella abortus". Arch. Biochem. Biophys. 49 (2): 424-33. doi:10.1016/0003 ...

*Ochrobactrum intermedium

nov., a new species with a closer relationship to Brucella spp". International Journal of Systematic Bacteriology. 48 (3): 759- ... "Evaluation of the relatedness of Brucella spp. and Ochrobactrum anthropi and description of Ochrobactrum intermedium sp. ...

*Swine brucellosis

The Brucella abortus (rough LPS Brucella) vaccine, developed for bovine brucellosis and licensed by the USDA Animal Plant ... Since brucellosis threatens the food supply and causes undulant fever, Brucella suis and other Brucella species (B. melitensis ... of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and Brucella ... "Brucella abortus cyclic beta-1,2-glucan mutants have reduced virulence in mice and are defective in intracellular replication ...
The genus Brucella contains highly infectious species that are classified as biological threat agents. The timely detection and identification of the microorganism involved is essential for an effective response not only to biological warfare attacks but also to natural outbreaks. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) is a rapid method for the analysis of biological samples. The advantages of this method, compared to conventional techniques, are rapidity, cost-effectiveness, accuracy and suitability for the high-throughput identification of bacteria. Discrepancies between taxonomy and genetic relatedness on the species and biovar level complicate the development of detection and identification assays. In this study, the accurate identification of Brucella species using MALDI-TOF-MS was achieved by constructing a Brucella reference library based on multilocus variable-number tandem repeat analysis (MLVA) data. By comparing MS-spectra from Brucella
Curated}} {{Biorealm Genus}} [[Image:brucella_close_up.JPG,thumb,400px,right,Electron micrograph of B. abortus located inside cisternae of Vero cell. Within the perinuclear envelope, the cisternae containing B. abortus are discontinuously lined by ribosomes (arrows). From [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2114362 Detilleux et al.]]] ==Classification== ===Higher order taxa:=== Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Brucellaceae ===Species:=== Brucella abortus, Brucella canis, Brucella cetaceae, Brucella maris , Brucella melitensis, Brucella pinnipediae, Brucella sp. {, , height="10" bgcolor="#FFDF95" align="center" , NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?id=234 Taxonomy] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=genomeprj&cmd=Search&dopt=DocSum&term=txid234%5BOrganism:exp%5D Genomes] ,} ==Description and Significance== Brucella sp. causes brucellosis, ...
An anti-Brucella vaccine candidate which comprises of purified Brucella lipopolysaccharide and cocktail of four Salmonella Typhimurium (ST)-Brucella vectors was reported earlier. Each vector constitutively express highly conserved Brucella antigens (rB), viz. lumazine synthase (BLS), proline racemase subunit A (PrpA), outer membrane protein-19 (Omp19), and Cu-Zn superoxide dismutase (SOD). The present study determined relative level of protection conferred by each single strain. Upon virulent challenge, the challenge strain was recovered most abundantly in non-immunized control mice and the ST-Omp19, ST-BLS, LPS, and ST-SOD immunized mice groups showed much less burden. Indirect ELISA based assay also confirmed the induction of antigen specific IgG for each antigen delivered. In route-wise comparison of the combined vaccine candidate, intraperitoneal (IP), intramuscular (IM), and subcutaneous (SC) immunizations revealed an indication of highly efficient routes for the protection. Splenocytes of ...
The primers for the genes of the identified proteins were designed and amplificated by PCR in four Brucella strains. The optimized PCR conditions and the length of PCR products were determined. The results of the PCR amplification in four different Brucella strains were shown." +", Corresponding sequence have been able to amplify; "-", Corresponding sequence not been able to amplify ...
Objective To study antimicrobial resistance of Brucella melitenesis and instruct rational use of antimicrobial agents for clinic.Methods Bacteria were cultured and identified by Bact/Alert 3D and VITEK Ⅱ automicrobic system.Drug susceptibility was detected by E-test method.Results Four strains Brucella melitenesis were isolated from blood of 4 patients with fever and MIC of gentamicin,ampicillin and ciprofloxacin was(0.5)~(0.75 mg/L),(1.5)~(2.0 mg/L) and (4.0)~(8.0 mg/L) respectively.MIC of ceftazidime,amikacin and doxycycline were all above(8.0 mg/L).Conclusion The preferred drug for treating brucellosis is gentamicin or plus ampicillin or ciprofloxacin,or choosing antimicrobial agents according to antimicrobial susceptibility test results.
This test looks for brucellosis, an infectious disease usually caused by handling animals or milk products infected with the brucella bacteria.
This test looks for brucellosis, an infectious disease usually caused by handling animals or milk products infected with the brucella bacteria.
Interpretive Summary: Brucella are intracellular pathogens that cause reproductive losses in cattle and zoonotic infections in people. Live attenuated vaccines have been available for protecting domestic livestock against brucellosis for more than 60 years. Current vaccines are effective in preventing abortion and transmission of brucellosis, but poor at preventing infection or seroconversion. Current knowledge suggests that both the innate and adaptive immune responses contribute to immunity against intracellular pathogens and that binding of pathogen structures onto pattern recognition receptors (PMRs) is critical to development of protective immunity. The observations that killed bacteria or subunit vaccines do not appear to fully stimulate PMRs or mimic intracellular trafficking of live Brucella, may explain their inability to induce protection that equals that provided by live vaccines. Brucella appear to have multiple mechanisms that subvert innate and adaptive immunity and prevent or ...
Brucellosis is primarily a worldwide zoonotic disease caused by Brucella species. The genus Brucella contains highly infectious species that are classified ...
Kang, Yoon-Suk, Daniel A. Brown, and James E. Kirby. "Brucella neotomae Recapitulates Attributes of Zoonotic Human Disease in a Murine Infection Model." Infection and Immunity 87.1 (2019): e00255-18. Web. 08 April. 2020. ...
When and how often laboratory tests are done may depend on many factors. The timing of laboratory tests may rely on the results or completion of other tests, procedures, or treatments. Lab tests may be performed immediately in an emergency, or tests may be delayed as a condition is treated or monitored. A test may be suggested or become necessary when certain signs or symptoms appear. Due to changes in the way your body naturally functions through the course of a day, lab tests may need to be performed at a certain time of day. If you have prepared for a test by changing your food or fluid intake, lab tests may be timed in accordance with those changes. Timing of tests may be based on increased and decreased levels of medications, drugs or other substances in the body. The age or gender of the person being tested may affect when and how often a lab test is required. Chronic or progressive conditions may need ongoing monitoring through the use of lab tests. Conditions that worsen and improve may ...
PCR-RFLP analysis of Brucella LPS genes manAO-Ag, manBO-Ag, wbkD, wbkF, wboA and wa**. Panel A. Lanes: 1, molecular size markers; 2, manAO-Ag from B. melitensis
This Website, does not provide medical advice,diagnosis or treatment. Content (text, video) on this website is only intended to provide general information to the reader and for academic purpose of medical students. Please consult your doctor for medical advice, diagnosis and treatment.We do not undertake any responsibility or liability of any health issues caused by following advise on this ...
Linux situations who revived emerging be might so track ed us peridural about innovative newsletters on principles, but they gained to be in various, and also had available to the download. I are that this environment - learning in always numerous secondgeneration and constellation - However is Ubuntu arts via the Ubuntu types, rightly is. being a text state is easily somewhere French with stifling in cancer not, and with Europeana sports - it is to evaluate especially Georgian with collection, with progression to the heir, with states and with escalating what a Jazz only means, or how it does or is itself.
A free platform for explaining your research in plain language, and managing how you communicate around it - so you can understand how best to increase its impact.
Spleen cells from mice infected with the rough Brucella melitensis strain B115 were fused with NSO myeloma cells. Hybridoma supernatants were screened in ELISA with cell walls (CW), sonicated cell extracts (CE) and rough lipopolysaccharide (R-LPS) of B. melitensis strain B115 and whole B. melitensis B115 cells. Surprisingly, 22 monoclonal antibodies (mAbs) reacting in ELISA with both CW and CE but not with R-LPS and bacterial cells were shown by immunoblot analysis and ELISA to react with smooth lipopolysaccharide (S-LPS). These mAbs also reacted in ELISA with O polysaccharides (OPS) from the smooth Brucella abortus strain 99 and the smooth B. melitensis strain 16M and thus recognize epitopes present on the O-chain. Proteinase K LPS preparations from B. melitensis B115 analysed by immunoblotting with one mAb (12G12) recognizing S-LPS of both A and M specificity displayed the typical S-LPS high-molecular-mass ladder pattern but no S-LPS was detected in the phenol/water/chloroform/light petroleum LPS
Pathogens infecting mammalian cells have developed various strategies to suppress and evade their hosts defensive mechanisms. In this line, the intracellular bacteria that are able to survive and propagate within their host cells must have developed strategies to avert their hosts killing attitude. Studying the interface of host-pathogen confrontation can provide valuable information for defining therapeutic approaches. Brucellosis, caused by the Brucella strains, is a zoonotic bacterial disease that affects thousands of humansand animals around the world inflicting discomfort and huge economic losses. Similar to many other intracellular dwelling bacteria, infections caused by Brucella are difficult to treat, and hence any attempt at identifying new and common therapeutic targets would prove beneficial for the purpose of curing infections caused by the intracellular bacteria. In THP-1 macrophage infected with Brucella melitensis we studied the expression levels of four hosts genes, i.e. EMP2, ST8SIA4
Looking for online definition of brucellae in the Medical Dictionary? brucellae explanation free. What is brucellae? Meaning of brucellae medical term. What does brucellae mean?
Brucellosis is a contagious disease of animals that also affects humans. The disease is also known as Bangs Disease. In humans, its known as Undulant Fever.. Brucellosis is one of the most serious diseases of livestock, considering the damage done by the infection in animals. Decreased milk production, weight loss, loss of young, infertility, and lameness are some of the affects on animals.. The Brucella species are named for their primary hosts: Brucella melitensis is found mostly is goats,sheep and camels, B. abortus is a pathogen of cattle, B. suis is found primarily in swine and B. canis is found in dogs.. There are two common ways people get infected with brucellosis. First, individuals that work with infected animals that have not been vaccinated against brucellosis. This would include farmers, slaughterhouse workers and veterinarians.. Related: College microbiology classes linked to Salmonella Typhimurium outbreak. They get infected through direct contact or aerosols produced by the ...
The current investigation is associated with the third known case of brucellosis from Brucella RB51 in a New York resident who who drank raw milk purchased from Millers Biodiversity Farm in Quarryville, Pennsylvania, was diagnosed with brucellosis in November 2018.. Milk samples from the dairy tested positive for Brucella strain RB51. People who consumed raw milk or raw milk products from this dairy since January 2016 may have been exposed.. As of January 22, 2019, exposures have been identified in 19 states: Alabama, California, Connecticut, Florida, Georgia, Iowa, Maryland, Massachusetts, Michigan, Minnesota, Mississippi, New Jersey, New York, North Carolina, Ohio, Pennsylvania, Rhode Island, South Carolina, and Virginia.. CDC notes that Brucella strain RB51 is a live-attenuated cattle vaccine strain, which can be shed in milk and can cause infections in humans. RB51 is resistant to rifampin and penicillin. There is no serological test available to detect RB51 infection. Blood culture is the ...
Rabbit polyclonal antibody raised against full length recombinant Brucella suis vjbR. Recombinant protein corresponding to full length Brucella suis vjbR. (PAB16064) - Products - Abnova
In 1999, a researcher suffered headaches, lassitude and a severe sinusitis after exposure to marine mammal strains of Brucella with which the worker was in contact. The symptoms resolved in one week after treatment with doxycycline and rifampin. The researcher had a positive titer for Brucella, and the organism was cultured from blood samples. PCR-RFLP was used to positively identify the isolates as being comparable to marine mammal Brucella (Brew and Staunton 1999). Organisms Brucella ssp. are gram-negative intracellular bacteria and are a major source of zoonoses worldwide. B. melitensus, B. abortus, and B. suis are some species commonly recognized to play a role in human and animal health. Nomenclature for marine mammal strains of Brucella has not yet been fully developed, but B. maris and B. pinnipedia have so far been named. Transmission occurs primarily through contact with aborted fetal material, and consumption of contaminated milk. ...
What is brucellosis? Brucellosis is an infectious disease caused by the bacteria of the genus Brucella. These bacteria are primarily passed among animals, and they cause disease in many different vertebrates. Various Brucella species affect sheep, goats, cattle, deer, elk, pigs, dogs, and several other animals. Humans become infected by coming in contact with animals or animal products that are contaminated with these bacteria. In humans brucellosis can cause a range of symptoms that are similar to the flu and may include fever, sweats, headaches, back pains, and physical weakness. Severe infections of the central nervous systems or lining of the heart may occur. Brucellosis can also cause long-lasting or chronic symptoms that include recurrent fevers, joint pain, and fatigue.. Back to Top How common is brucellosis? Brucellosis is not very common in the United States, where100 to 200 cases occur each year. But brucellosis can be very common in countries where animal disease control programs have ...
Brucella canis is a Gram-negative proteobacterium in the family Brucellaceae that causes brucellosis in dogs and other canids. B. canis is rod-shaped or a coccus, and is oxidase, catalase, and urease positive. The species was firstly described in United States in 1966 where mass abortions of beagles were documented. The disease is characterized by epididymitis and orchitis in male dogs, endometritis, placentitis, and abortions in females, and often presents as infertility in both sexes. Other symptoms such as inflammation in the eyes and axial and appendicular skeleton; lymphadenopathy and splenomegaly, are less common. Humans can be also infected, but occurrences are rare. B. canis is a zoonotic organism. Signs of this disease are different in both genders of dogs; females that have B. canis infections face an abortion of their developed fetuses. Males face the chance of infertility, because they develop an antibody against the sperm. This may be followed by inflammation of the testes which ...
Neutralophilic bacteria have developed specific mechanisms to cope with the acid stress encountered in environments such as soil, fermented foods- and host compartments. In Escherichia coli, the glutamate decarboxylase (Gad)-dependent system is extremely efficient: it requires the concerted action of glutamate decarboxylase (GadA/GadB) and of the glutamate/GABA antiporter, GadC. Notably, this system is operative also in new strains/species of Brucella, among which Brucella microti, but not in the
Oceans and seas are the widest sources of biological and chemical diversity representing a prolific reserve of unexploited and/or unknown microorganisms [1, 2]. Thus, marine environments are great resources of new substances having potential applications in pharmaceutical, feed and food, fine chemicals and enzyme industries [2, 3]. The search of new microorganisms, having unique physiological and metabolic capabilities, aids to better comprehend the ecosystem and provides opportunities to discover new compounds of commercial importance. This is particularly true for marine bacteria that have been less studied than their terrestrial counterpart and are often underrated or completely ignored by many scientists [4, 5].. Among the microbial products, exopolysaccharides (EPS) have many important physiological functions and various practical applications deductible from their roles in nature.. These high molecular weight polymers represent essential components of the secreted extracellular material ...
Recent studies show that FDG-PET may contribute for precise grading and prognosis in different solid tumors, including soft tissue sarcomas [5], as high grade tumors show a much higher uptake of 18F-FDG due to a higher glycolytic phenotype [3]. Also, it was recently described that FDG-PET should be considered an important imaging modality for therapeutic monitoring in patients with high-grade STS [6, 7]. In this context, metabolic characterization of STS emerges as a possibly relevant approach for STS management, with therapeutic implications as early treatment decisions such as discontinuation of chemotherapy in non-responding patients could be based on FDG-PET criteria [7]. Importantly, the hyperglycolytic phenotype present in this type of tumors, similarly to other solid tumors, may be the basis for the use of new directed therapeutic strategies which are currently in clinical trials [29]. Therefore, tumor metabolic characterization, including MCTs as responsible for lactate efflux from ...
Brucellosis is one of the most widespread zoonosis in the world. In China, 90% of human brucellosis occurs in six northern agricultural provinces. However, there is a recent increase in the trend of human brucellosis in southern provinces with limited cases reported in the literature. Our study aims to describe the clinical features and epidemiology of brucellosis in a tertiary hospital in southern China. A retrospective case series of brucellosis was conducted between January 1, 2014 and October 31. 2018. Cases were identified based on positive Brucella serology by tube agglutination test, or positive culture from clinical specimen identified by Vitek 2 and MALDL-TOF MS. Clinical details of brucellosis including patients occupation, risk factors, and complications were analyzed. Clinical characteristics between patients from Guangdong and other provinces were also compared. A total of 13 cases of laboratory-confirmed brucellosis were identified. 7 (53.8%) of the patients were male, 6 (46.2%) were
Abstract of Paper: Serodiagnosis Of Human Brucellosis : Validity Of Brucella-Specific IgM Antibodies Dipstick Test As A Screening Modality Of Febrile Patients , Author: Hosam Armen ; Mohamed El-Ass& & Jehan H. Sabry
Brucella BvrR protein: BvrR/BvrS represent the two-component system involved in the regulation of Brucella virulence; amino acid sequence in first source
Introduction & Objective: Brucellosis is an important zoonosis considered a serious hazard to public health . Human brucellosis is caused by one of the four species of the brucella genus: B. melitensis is principally found in goats and sheep, B. abortus in cattle, B. suis in swine and B. canis in dogs. ...
Brucellosis is a common bacterial zoonotic infection but data on the prevalence among humans and animals is limited in Kenya. A cross-sectional survey was conducted in three counties practicing different livestock production systems to simultaneously assess the seroprevalence of, and risk factors for brucellosis among humans and their livestock (cattle, sheep, camels, and goats). A two-stage cluster sampling method with random selection of sublocations and households was conducted. Blood samples were collected from humans and animals and tested for Brucella immunoglobulin G (IgG) antibodies. Human and animal individual seroprevalence was 16% and 8%, respectively. Household and herd seroprevalence ranged from 5% to 73% and 6% to 68%, respectively. There was a 6-fold odds of human seropositivity in households with a seropositive animal compared with those without. Risk factors for human seropositivity included regular ingestion of raw milk (adjusted odds ratio [aOR] = 3.5, 95% confidence interval ...
Among animals, brucellosis is transmitted directly (contact with placenta, aborted fetus, the liquor , vaginal and/or uterus secretion) or indirectly (if infected and non-infected health herds mix while grazing or by contact with environment in which the bacteria can be found such as manure, pastures, water, etc). Most infected male animals continue to secrete Brucella via semen for the lifetime. Presence of infectious agents in udder and surrounding lymph nodes results in permanent or occasional secretion of Brucella via milk and as such it is a serious source of infection for young animals which can also be infected via uterus. In infected lambs and young goats, Brucella inhabits lymph nods of bowls and can be secreted out of their body via droppings. Infected young cattle develop the disease once they reached sexual maturity. Sheppard dogs can also spread brucellosis between ruminants but they can also get ill as well. In high humidly, low temperature and unsunny weather conditions, Brucella ...
Symptomatic seropositive family members were treated, whereas asymptomatic seropositive family members were followed-up with no treatment unless they showed symptoms and an increase in STA titers. Of eight family members followed, two were diagnosed with acute brucellosis.. Discussion. Turkey has seven geographical regions and is an important migration route between Europe and Asia. There are significant differences between regions, in terms of geographical features and economic and social development. Due to the geographic situation, Turkey is a risk factor for many infectious diseases, such as brucellosis.11 A total of 189,226 cases of human brucellosis were officially reported between 2000 and 2005, of which approximately 90,000 were registered (approximately 15,000 cases per year) in Turkey. The highest seroprevalence was observed in the eastern and southeastern regions of the country.12 Despite being endemic in Turkey, brucellosis remains underdiagnosed due to its non-specific clinical ...
... is an infectious disease caused the Brucella bacteria which spread to humans from animals. Some Brucella bacteria strains are seen in cows, while some occur in pigs, dogs, sheep, camels and goats. This is the forum for discussing anything related to this health condition
On April 23, 2011, plaintiff Christine Brucella, 77, a retiree, alleged she was walking down an aisle at Shoprite supermarket in Paramus when another shopper struck her with his shopping cart, causing her to fall. Brucella claimed she sustained a fracture to her right hip in the incident. Brucella sued Shoprite of Paramus and its buying cooperative, Wakefern Food Corporation, for negligence. She claimed the aisle was too narrow to allow her to move out of the way of the shopping cart to avoid being hit by it. Plaintiffs counsel claimed the aisle created a dangerous condition for patrons who are walking when a shopper pushing a cart suddenly appears in front them. The plaintiffs liability expert testified that the aisle needed to be 12 feet wide to assure safe passage of patrons with shopping carts. The defense argued that the aisle was the appropriate size and was not a dangerous condition. Defense counsel noted that the accident was captured on surveillance video. Counsel contended that ...
The innate immune system detects the presence of microbes in tissue by pattern recognition of conserved microbial structures, known as pathogen-associated molec...
Investigations in Texas and New Jersey identified people infected with a dangerous germ called Brucella RB51. If not treated with antibiotics, Brucella RB51 infection can lead to serious chronic infections and complications. These illnesses occurred among people who drank raw (unpasteurized) milk from a dairy farm in Texas and a milk delivery service in the Northeast. CDC and state health officials warned customers not to drink raw milk from these dairies and advised those who consumed the raw milk to seek antibiotic treatment.. ...
Size, charge and structural heterogeneity of Brucella abortus lipopolysaccharides demonstrated by two-dimensional gel electrophoresis (pages 283-288). Dr. Blair A. Sowa, Richard P. Crawforda, Fred C. Heck, John D. Williams, Albert M. Wu, Katherine A. Kelly and L. Garry Adams. Version of Record online: 16 APR 2008 , DOI: 10.1002/elps.1150070608. ...
Antibiotic activity of molds for Brucella. The isolation of two active substances from a strain of Aspergillus terreus Thom: one in crystalline form, the other as an amorphous concentrate ...
We present a rare case of brucella endocarditis, forming a vegetation on the mitral valve. The definitive diagnosis has been made with clinical suspicion, positive serology, the demonstration of the vegetation with the echocardiography and with the production from the multiple blood culture of brucella melitensis and from the excised valve. Our patient has been successfully treated with specific antibiotherapy and the surgery of replacement of mitral valve. Our aim in presenting the case is to remind the infective endocarditis which is due to this factor in the regions like our country which is endemic for ...
Aim: We determined the antibody response in cattle naturally infected with brucellosis and normal healthy adult cattle vaccinated during calf hood with strain 19.. Materials and Methods: The antibody titers were measured by standard tube agglutination test (STAT), microtiter plate agglutination test (MAT), indirect hemagglutination assay (IHA), and indirect enzyme-linked immunosorbent assay (iELISA) as per standard protocols.. Results: The mean STAT titers were 1.963 0.345 in infected cattle and 1.200 0.155 in healthy vaccinated cattle. The difference was extremely significant (p,0.0001). The mean MAT titers were 2.244 0.727 in infected cattle and 1.200 0.155 in healthy vaccinated cattle. The difference was very significant (p,0.005). The mean IHA titers in infected cattle were 2.284 0.574, and those in healthy vaccinated cattle were 1.200 0.155. The difference was extremely significant (p=0.0002). However, the difference in mean iELISA titers of infected cattle (1.3678 0.014) and healthy ...
The less mucoid strain of Brucella canis or M- strain is used for the serologic diagnosis of canine brucellosis. While this strain is avirulent in dogs, we report the case of clinical brucellosis that developed in a laboratory worker a few days after ...
Abstract Of 1,065 persons sampled during a house-to-house survey of an area of the Department of Curuzú Cuatiá, Province of Corrientes, Argentina, 21 were seropositive to Brucella canis antibodies by the gel-diffusion test, using a saline-extracted B. ovis surface R antigen. Two positive reactors were similarly identified during a survey of rural schools in the Province of Neuquén, which included 887 persons. The findings are discussed in terms of current knowledge of this recently recognized zoonotic infection.
Sheep are considered the primary source of Brucella mellitensis, which is the most pathogenic Brucella sp. in humans and the predominant strain circulating in Middle East, including Egypt [22, 23]. Recent non-governmental studies indicate that brucellosis is highly endemic in ruminants in Egypt, though large discrepancies in seroprevalence exist between peer-reviewed published studies and those reported by the government [9, 12].. The seroprevalence of brucellosis in sheep in the study area was estimated at 20 % (95 % CI 15.3-24.7 %). Official Egyptian government figures nationwide for Brucella seroprevalence in sheep between 1999 and 2011 range from 0.5 to 2.5 % [15]. The seroprevalence in this study is slightly higher than that reported by Hegazy et al. [13]. This study agrees with Hegazy et al. [13] stating that brucellosis is endemic in Egypt with a high seroprevalence (around 15 %) despite the current national control programme. This may be due to poor availability of resources, a lack of ...
95%). Cardiovascular involvement is low (endocarditis at 2%), but is the major cause of mortality. Often, valve replacement and antibiotics are needed. Pericarditis and myocarditis are seen, too. Pulmonary infection can be from inhalation or hematogenous sources, and can cause any chest syndrome. Rarely is Brucella isolated from sputum. Genitourinary infection can include epidydemoorchitis or pyonephrosis (rare). Cutaneous involvement is not specific. Hematological signs include anemia, leukopenia, and thrombocytopenia. Brucella is isolated from a blood culture on Castaneda medium or from bone marrow. Prolonged incubation (up to six weeks) may be required, as they are slow-growing, but on modern automated machines, the cultures often show positive results within seven days. On Gram stain, they appear as dense clumps of Gram-negative coccobacilli and are exceedingly difficult to see. In recent years, molecular diagnostic techniques based on the genetic component of the pathogen have become more ...
Isolated case reports of peritonitis due to Brucella spp. during peritoneal dialysis (PD) continue to surface in the medical literature. However, the optimal treatment regimen for these patients, in particular with regards to the fate of PD catheter, is still largely unknown. We report a case of brucella peritonitis successfully treated with intraperitoneal administration of amikacin, along with oral rifampicin and doxycycline but without catheter removal. Furthermore, we have reviewed the literature up until present day.
Random gene inactivation used to identify cellular functions associated with virulence and survival of Brucella spp has relied heavily upon the use of the transposon Tn5 that integrates at G/C base pairs. Transposons of the mariner family do not require species-specific host factors for efficient transposition, integrate nonspecifically at T/A base pairs, and, at a minimum, provide an alternative approach for gene discovery. In this study, plasmid vector pSC189, containing both the hyperactive transposase C9 and transposon terminal inverted repeats flanking a kanamycin resistance gene, were used to deliver Himar1 transposable element into the B. melitensis genome. Conjugation was performed efficiently and rapidly in less than one generation in order to minimize the formation of siblings while assuring the highest level of genome coverage. Although previously identified groups or classes of genes required for virulence and survival were represented in the screen, additional novel identifications were
Brucellosis affects mainly farm animals but dogs may harbor the bacteria that cause the disease and spread it to other animals and people. Dog-to-human transmission is rare; the risk is higher for people who work around animals and pets - for veterinarians, for example - that it is for pet owners. Only a couple of hundred cases occur in the United States each year. The most common way of contracting the disease is eating or drinking contaminated milk products. Americans sometimes get brucellosis from eating unpasteurized milk or cheese while traveling in certain parts of Europe, Asia, and Africa. The disease is caused by bacteria in the genus Brucella. The species that infects dogs is called Brucella canis. The symptoms are similar to those produced by influenza: fever, headaches, back pain, and physical weakness. In severe cases, the infection gets into the central nervous system or lining of the heart. (Locked) More » ...
Brucella abortus is a Gram-negative, facultative intracellular bacterium that causes brucellosis, a worldwide zoonotic disease leading to undulant fever in humans and abortion in cattle. The immune response against this bacterium relies on the recognition of microbial pathogen-associated molecular patterns, such as lipoproteins, lipopolysaccharides and DNA; however, the immunostimulatory potential of B. abortus RNA remains to be elucidated. Here, we show that dendritic cells (DCs) produce significant amounts of IL-12, IL-6 and IP-10/CXCL10, when stimulated with purified B. abortus RNA. IL-12 secretion by DCs stimulated with RNA depends on TLR7 while IL-6 depends on TLR7 and partially on TLR3. Further, only TLR7 plays a role in IL-12 production induced by B. abortus infection. Moreover, cytokine production in DCs infected with B. abortus or stimulated with bacterial RNA was reduced upon pretreatment with MAPK/NF-κB inhibitors. By confocal microscopy, we demonstrated that TLR7 is colocalized with B.
Canine Brucellosis. By Admin. Canine Brucellosis is an infectious disease that is caused by a bacteria in dogs called Brucella Canis. It resides in a dogs reproductive organs. Brucellosis is one of the main causes of failure in dogs reproductive organs. It is a leading cause of late abortions which occur forty five to fifty five days of gestation It may be the cause of a bitch delivering stillborn puppies or puppies that sicken and die soon after birth. It can cause sterility in a male and female dog without causing obvious signs of the disease.. Dogs with active infectious bacteria may show enlargement of the lumpy nodes in the groin or below the jaw in association with febrile illness. Eye inflammation may be seen in both sexes. The dogs joints may become swollen and painful. The males testicles may swell up, then go on to atrophy as the sperm producing cells are destroyed. In some dogs, the disease goes unsuspected until there is evidence of reproductive failure. These dogs are in a ...
Disrupting the ure1 operon causes loss of urease activity, decreased resistance to low pH killing in vitro and decreased pathogen survival when inoculated in BALB/c mice by gavage.
Dendogram of global Brucella abortus biovar 1 genotypes. Dendogram based on MLVA-16 genotyping showing the relationship of 49 B. abortus biovar 1 strains origin
In this study, we explore the regulatory roles of pro-inflammatory cytokine tumor necrosis factor alpha (TNF) in the innate immunity of macrophages against B. abortus infection. We show that infection of macrophage with B. abortus induces marked expression and secretion of TNF which subsequently binds to TNF receptor 1 (TNFR-1) and activates a downstream signaling cascade of the innate immunity. Blocking of TNF signaling resulted in a notable increase of B. abortus survival which was associated with an increase of anti-inflammatory cytokine interleukin 10 (IL-10), a beneficial effector of Brucella survival, as well as remarkable decrease of reactive oxygen species (ROS) and nitric oxide (NO), antibrucella molecules ...
Brucellosis is a worldwide zoonotic disease transmitted to humans by consumption of contaminated milk and milk products. Brucellosis is endemic in Turkey, and Edirne has a high Brucella prevalence. Brucellosis is prevented by live-attenuated vaccines for animals and the vaccination program has been in place since 1984 in Turkey. Thrace is the pilot region for this vaccination program. The gold standard diagnostic technique for brucellosis is still the isolation of suspicious bacterial colonies followed by bacteriological identification, but it is very time consuming and laborious. In many studies, Brucella has been investigated by PCR techniques. However, PCR-based methods cannot differentiate between the vaccine strain and the virulent strain; thus, the vaccine strain may interfere with the virulent strain and causes false-positive reactions. To monitor brucellosis control programs effectively, it is important to distinguish vaccine and field strains of Brucella spp. In this study, raw milk ...
Brucellosis is a contagious bacterial disease that affects several animal species. In dogs, this condition is caused by a bacterium known as Brucella canis.
... is a bacterial disease that often leads to joint pain (arthralgia), muscle pain (myalgia), fever, headache, and weakness. The bacterium can live inside animals and can be spread to people. Brucellosis is uncommon in the United States (around 100 cases per year are reported), but it can cause serious illness, especially in pregnant women, immunocompromised individuals, infants, and the elderly.. Brucellosis is reportable to the Iowa Department of Public Health by Iowa Administrative Code 641 IAC 1.. ...
antibody finding of tularemia patients within the period from 1 to 11 years since the beginning of the disease. 3. Assesment of efficiency of the applied antibiotic therapy on patients suffering from tularemia. Methods: Testing was performed on a group of 113 patients suffering from tularemia in a period starting in 1999.until the end of 2011. Control group included 111 examinees, out of which 31 patient with differential diagnosis of lymphadenopathy of different origin, 20 seropositive brucella patients, 10 seronegative brucella patients, 30 seropositive yersinia patients and 20 voluntary blood donors. During the diagnostic processing of patients, following materials were used: serum and bioptat of lymph node. Total of 270 serum samples were examined, out of which 189 serums suspicious of tularemia, 111 serums from the control groupe and 26 lymph node bioptats. There were 8 diagnostic techniques used on the material mentioned, out of which 6 serological, imunohistochemical method of indirect ...
FUN_05: Use the SSGCID solved structure of Brucella melitensis methionine-tRNA-synthetase (MetRS) to find inhibitors for potential drug development for brucellosis.
JE Hyung Gon , SONG Hyun Circulation journal : official journal of the Japanese Circulation Society 72(3), 500-501, 2008-02-20 J-STAGE 医中誌Web 参考文献8件 ...
En tres municipios de Córdoba (Andalucía, España) se identificaron once casos de brucelosis. Se realizó un estudio de casos y controles, seleccionando tres casos por cada control. Las personas que consumieron queso de cabra fresco sin higienizar elaborado en una granja situada en el territorio epidémico, presentaban un mayor riesgo de padecer brucelosis (OR=21,6, IC 95%=1,6-639,8). Se identificó Brucella melitensis serotipo 3 en muestras clínicas, y en tejido de cabras y muestras de leche de la granja del rebaño. Se adoptaron medidas preventivas y el brote fue contenido tras la retirada del mercado de todos los quesos sospechosos, la desinfección y limpieza adicional de la granja y la realización de actividades de promoción sanitaria.
Description of Brucellosis, what is is, how it is transmitted, which countries have Brucellosis and what can you do to prevent it
Antibiotics such as tetracyclines, rifampicin, and the aminoglycosides streptomycin and gentamicin are effective against Brucella bacteria. However, the use of more than one antibiotic is needed for several weeks, because the bacteria incubate within cells.. Surveillance using serological tests, as well as tests on milk like the milk ring test, can be used for screening and play an important role in campaigns to eliminate the disease. As well individual animal testing both for trade and for disease control purposes is practiced. In endemic areas, vaccination is often used to reduce the incidence of infection. Several vaccines are available that use modified live viruses. The World Organisation for Animal Health Manual of Diagnostic Test and Vaccines for Terrestrial Animals provides detailed guidance on the production of vaccines.As the disease is closer to being eliminated, a test and stamping out program is required to completely eliminate it.. The gold standard treatment for adults is daily ...
Trouvez tous les livres de Young, Edward J.; Corbel, Michael J.; Young, Young J. - Brucellosis: Clinical and Laboratory Aspects. Sur eurolivre.fr,vous pouvez commander des livres anciens et neufs.COMPARER ET acheter IMMÉDIATEMENT au meilleur prix. 9780849366611
The Brucella abortus strain S19, a spontaneously attenuated strain, has been used as a vaccine strain in vaccination of cattle against brucellosis for six decades. Despite many studies, the physiological and molecular mechanisms causing the attenuation are not known. We have applied pyrosequencing technology together with conventional sequencing to rapidly and comprehensively determine the complete genome sequence of the attenuated Brucella abortus vaccine strain S19. The main goal of this study is to identify candidate virulence genes by systematic comparative analysis of the attenuated strain with the published genome sequences of two virulent and closely related strains of B. abortus, 9-941 and 2308. The two S19 chromosomes are 2,122,487 and 1,161,449 bp in length. A total of 3062 genes were identified and annotated. Pairwise and reciprocal genome comparisons resulted in a total of 263 genes that were non-identical between the S19 genome and any of the two virulent strains. Amongst these, 45 genes
A serological survey of Brucella abortus in cattle originating from communal grazing areas of Kwa Zulu Natal was carried out between March 2001 and December 2003. The survey was designed as a 2-stage survey, considering the diptank as the primary sampling unit. In total 46 025 animals from 446 diptanks of 33 magisterial districts were sampled and tested using the Rose Bengal test and Complement Fixation Test. The apparent prevalence at district level was adjusted for clustering, diagnostic test sensitivity and specificity, and mapped using ArcView version 3.3. The prevalence of brucellosis in communal grazing areas of Kwa-Zulu Natal was found to be 1.45 % (0.84-2.21 %) and varied from 0 to 15.6% between magisterial districts. In 19 of the 33 magisterial districts no serological reactors were observed. A large variation in prevalence was found within diptank areas. Brucellosis was found to be most prevalent in the northeastern area of the province. The findings of the survey are discussed ...
ABSTRACT. In this study, milk and blood samples collected simultaneously from 532 trade cows to be slaughtered at Bodija abattoir, Ibadan (southwestern, Nigeria) were examined for antibodies to Brucella using the milk ring test (MRT) and the rose bengal test (RBT). Overall, 18.61 % of the milk samples were positive according to the MRT, while 9.77 % of the serum samples were positive according to the RBT. The difference was highly significant (Chi-square value 16.33; P , 0.05); only 32 (6.02 %) of the samples were positive for both tests. The Red Bororo breed of cattle and the White Fulani had the highest positive rates, namely 20.93 % and 11.69 % for the MRT and RBT respectively. No conclusion can be drawn about sensitivity because we do not know the true status of the animals tested. It is, however, obvious that although the MRT and RBT are 1st-line screening tests for brucellosis in cows in some countries, their lack of specificity is of concern. Therefore, the requirement for other ...
Study of diagnostic efficacy of widal slide agglutination test against widal tube agglutination test in enteric fever-IJMR-Print ISSN No:-2394-546X Online ISSN No:-2394-5478Article DOI No:-10.18231,Indian Journal of Microbiology Research-IP Innovative Publication Pvt Limited, Medical Journals Publication, Open Access
The results of our study are in agreement with the reports of other workers1,3,15,17, who also demonstrated the presence of B. abortus agglutinins in dog sera using RBT. The higher prevalence of 5.46 % recorded by RBT compared to 0.27 % (only 1 animal) by RSA could be attributed to the practice of feeding dogs with foetuses from slaughtered cows or meat at abattoirs as the prevalence of brucellosis is 6 % in cattle in Nigeria5.. Although not conclusive, our data suggest that young adult dogs (,1 year ) are most affected (Table 1). This is corroborated by a report of higher rates in this age group1and a study that suggested that Brucella infection in dogs is age-dependent15. In addition, we recorded higher prevalences in females (Table 2) but in another study a slightly higher rate in males (29.6 %) than in females (26.7 %) was recorded1. A contributing factor to higher rates in females could be that a single male dog, if infected and used to mate with several females, can transmit the infection ...
Three flocks (all Romney Marsh) out of 13 were found to be infected, suggesting the disease had been introduced through sheep importations from New Zealand. A study of clinical lesions in the genital tract and of seminal quality made in these and in five of the uninfected flocks showed that Br. ovis infection is associated with a high incidence of epididymitis and orchitis, and a general deterioration in seminal quality with secondary morphological abnormalities of the spermatozoa being a prominent feature ...
Background: Lipopolysaccharides (LPSs) and several antigenic proteins of Brucella have been considered for preparation of diagnostic reagents and subunit vaccines. The objective of this study was to identify and compare immunogens of B. abortus S19 which induce humoral immune responses in human, goat and rabbit. Material and Methods: The bacterial whole cell extract was prepared in extraction buffer and resolved using two-dimensional gel electrophoresis (2-DE). The resolved antigens were reacted against human, goat and rabbit sera using western blotting. Results: At least 19, 14 and 16 immunogenic proteins were recognized in western blotting with human, goat and rabbit sera, respectively. The most abundant proteins of the bacterium with immunogenic properties in goat and rabbit but not in human, were a group of 5-6 proteins with molecular masses of 32-34 KDa and isoelectric point (pI) ranging from 4.5 to 5.7. In contrast, a group of 5 proteins with molecular weight of 45 KDa and pI in the range of 4.5
Ochrobactrum anthropi is a common soil alphaproteobacterium (7) that can interact with or colonize many eukaryotic organisms, leading to different outcomes, such as disease or even growth of some plants (references 4 and 13 and references therein). O. anthropi, a close relative of brucellae, has not garnered much attention (5, 8) but is becoming increasingly recognized as a potentially problematic opportunistic and nosocomial pathogen (13). A rising number of reported cases includes some potentially life-threatening infections, such as endocarditis (6, 9, 14). These reports, together with the organisms intrinsic multiresistance to antibiotics (13), could lead to a situation resembling that of an Acinetobacter sp., whose importance as a highly problematic nosocomial pathogen skyrocketed in the past few years (12). The increasing relevance of O. anthropi to human health, together with its phylogenetic proximity to the highly pathogenic brucellae (a USDA Select Agent), prompted us to determine the ...
We recently noted, in performing a metabolic characterization of mice deleted for LKB1 in the endocrine pancreas and a restricted set of CNS neurons using a RIP2-Cre transgene (Sun et al., 2010b), that older animals became paralyzed. The principal aim of the present study was thus to dissect the pathology behind this change and, in doing so, to determine the role of LKB1 in regulating neuronal polarity and survival in the CNS in vivo.. Although mice null for LKB1 throughout the body die before E11.5, the use of an Emx1-Cre deleter strain to allow deletion in pyramidal neuron progenitors demonstrated that LKB1 is required for the polarization of cultured neurons from the neonatal hippocampus and cortex (Barnes et al., 2007; Shelly et al., 2007). We therefore reasoned that LKB1 might play a similar role in axon development and, importantly, in signal transmission along the spinal cord. Given the crucial role of the spinal cord for the normal control of motor function, we further reasoned that ...
Services The Islet and Physiology Core of the Indiana Diabetes Research Center provides IUSM and regional (IUB, IUPUI, and Purdue University) investigators with the capability to obtain high quality rodent pancreatic islets for study. The Core also provides services for islet transplantation and will assist investigators who wish to perform immunohistochemistry, immunofluorescence, and/or analysis of beta cell mass on whole pancreata from mouse and rat models. We are also pleased to offer several services for rodent metabolic characterization, including performance of insulin and glucose tolerance testing, analysis of body composition and metabolic cage analysis using the TSE System cages. The Islet and Physiology Core functions as part of the Indiana Diabetes Research Center and the IUSM Center for Diabetes and Metabolic Diseases, to foster new knowledge, support training, and promote basic and translational research in diabetes and related metabolic disorders and their complications​. ...
Diagnosis Code A23.0 information, including descriptions, synonyms, code edits, diagnostic related groups, ICD-9 conversion and references to the diseases index.
Brucellosis generally presents with fever, malaise, weight loss and bone pain with either an abrupt or insidious onset. A 76-year-old man presented in April 2010 with fever of 103°F, severe tachycardia, tachypnoea and a blood pressure of 80/50 mm Hg requiring fluids and vasopressor support with norepinephrine. The patient had brucellosis in 1956 which was treated for many weeks with tetracycline and streptomycin. He has had no recurrences since that time. He denies recent travel outside the USA or consumption of raw dairy products. Blood cultures grew Brucella melitensis. He was treated with gentamycin, doxycycline and rifampin for 1 week and discharged home on doxycycline and rifampin. He relapsed after 2 days, requiring re-admission and a 4-week course of gentamycin. This case is most unusual in that the brucellosis presented with septic shock after a 50-year quiescence and required prolonged therapy with gentamycin to induce remission. ...
There are four species of the Gram-negative, aerobic brucella bacillus, each comprising several biovars: Brucella melitensis (Malta fever, most commonly associated with goats, sheep, and camels), B. abortus (cattle), B. suis (pigs), and B. canis (dogs). The disease that they cause-brucellosis-occurs worldwide, but is especially prevalent in the Mediterranean region, the Indian subcontinent, Mexico, and Central and South America. Transmission is commonly by ingestion of untreated dairy products or other contaminated foods, but can also be by inhalation or inoculation....
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Brucellosis, Undulant fever, Mediterranean fever or Malta fever is different wards for one disease which is an infectious disease caused by a type of bacteria called Brucella , that are almost invariably transmitted by direct or indirect contact with infected animals or their products. Additionally, human brucellosis has a serious medical impact worldwide, which affects people of all age groups and of both sexes. The eradication poses major difficulties because this disease is largely under diagnosed and underreported. Furthermore, brucellosis in human beings is rarely fatal; it can be severely debilitating and disabling. A renewed scientific interest in human brucellosis has been fuelled by its recent re-emergence and enhanced surveillance in many areas of the world because of regarding the disease as a class B bioterrorist agent and remains the world?s most common bacterial zoonosis. A cross-sectional study was conducted in the period from March, 2013 to March, 2014 to estimate seroprevalence ...
The goal of our proposed research is to develop a Brucella vaccine that is safe for human use. Our approach has explored the use of live attenuated vaccines, si...
Toxoplasmosis, leptospirosis, and brucellosis are global zoonoses, with humans as accidental participants in their transmission cycles. The can also be considered occupational diseases, because certain professionals are at greater risk of contact and infection by such zoonoses. These three diseases have different epidemiological characteristics because of the distinct environmental, social, cultural, and economic conditions where these pathogens circulate. Because of the importance of these diseases and their associations with specific occupations, we performed a seroepidemiological survey of Toxoplasma, Leptospira, and Brucella antibodies, with an analysis of the association between positive serum and certain occupational and environmental variables, in students of a veterinary medicine course (1st, 2nd, 3rd, 4th, and 5th year) in a university in the northwestern region in the state of Parana, Brazil. From May to November 2014, blood samples were collected from 157 volunteers by professionals ...
See chapter 12 for further information. (357) This disease is caused by the bacterium Brucella suis. Infection of the pregnant sow may result in abortion. Generally it...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Powered by Pure, Scopus & Elsevier Fingerprint Engine™ © 2020 Elsevier B.V. We use cookies to help provide and enhance our service and tailor content. By continuing you agree to the use of cookies. Log in to Pure. ...
Abstract The penultimate step in the biosynthesis of riboflavin (vitamin B2) involves the condensation of 3,4-dihydroxy-2-butanone 4-phosphate with 5-amino-6-ribitylamino-2,4(1H,3H..
Brucellosis is one of the most important infectious diseases worldwide. Nevertheless, since it is not regarded as a priority by national and international health systems in many endemic regions, it is considered to be a neglected zoonosis. Measures to prevent and control brucellosis rely upon direct approaches aimed at minimising the risk of spreading infection among animals. Collectively, these measures tend to reduce the exposure of animals to Brucella spp. and to increase resistance to infection in susceptible animals. To implement an effective disease control strategy, detailed information about the presence of the pathogen in a specific territory is of fundamental importance. For that reason, particular emphasis should be placed on active surveillance using serological methods. Serological surveillance provides useful information to aid in understanding epidemiological patterns and assess the impact of brucellosis in the targeted area, paving the way to define the most suitable approaches ...
Brucellosis is often detected when cull cows are sent to market. If a cow tests positive for brucellosis antibodies in her blood, the herd will be placed under temporary quarantine for additional testing of all reproductive cattle. Any suspect cows are sent to slaughter, so tissues can be tested. If the Brucella bacteria can be grown from the cows tissues, the entire herd is placed under a written quarantine. This means the herd will not be allowed to use any pastures that would allow fence-line contact with other herds. The herd is quarantined until all test-eligible cattle pass three consecutive blood-tests. The final test must occur after the herd has finished calving ...
SPAIN – After 20 years of eradication efforts, four autonomous areas have been officially declared bovine Brucellosis free.
COLOMBIA – Advances are being made in delivering a tuberculosis and brucellosis ‘free’ programme to a southern region of the country, says the Colombian Agricultural Institute.
FIATECH established the CETI Award - Celebration of Engineering & Technology Innovation - in 2006 to promote and showcase innovative construction-related technologies that benefit the capital projects industry. The CETI Award is given annually to organizations that have conducted new and emerging technology implementations as well as research & development.
PANAMA – National Animal Health Officials are baffled as to the source of a Brucellosis outbreak centred on the western half of the country’s capital.
1EI5: Crystal structure of a D-aminopeptidase from Ochrobactrum anthropi, a new member of the penicillin-recognizing enzyme family.
Abstract. We report the draft genome sequences of two Brucella abortus strains LMN1 and LMN2 isolated from cattle and pig. The LMN1 and LMN2 have the genome size of 3,395,952 bp and 3,334,792 bp, respectively. In addition to the conserved genes of Brucella, few novel regions showing similarity to the phages were identified in both strains.. Keywords: genome sequence, Brucella abortus ...
Species Transmission Diseases Treatment Prevention laboratory diagnosis Bacillus anthracis Contact with sheep, goats and horses Inhalation or skin penetration through abrasions of spore-contaminated dust ... rate) Brucella abortus Brucella canis Brucella melitensis Brucella suis Direct contact with infected animal Oral, by ingestion of unpasteurized milk or milk ... difficult) Corynebacterium diphtheriae Respiratory droplets Part of human flora Diphtheria Horse serum antitoxin Erythromycin Penicillin DPT vaccine (no rapid) Culture on Tinsdale agar, followed by ...
Brucella abortus is a Gram-negative bacterium that causes abortion and infertility in food animals and a chronic debilitating febrile disease in humans known as brucellosis. As with all pathogenic bacteria, the Brucella spp. require sufficient metal nutrition during the course of an infection. Host-mediated metal withdrawal defenses actively restrict the bioavailability of metals which requires invading bacteria to employ high affinity metal acquisition systems to overcome these metal-limiting conditions. While obtaining sufficient metals during host infection is critical to the survival of these bacteria, avoiding metal toxicity is equally important. Excess accumulation of one metal relative to others can lead to protein mis-metallation when surplus metal ions outcompete other metal species for their native binding sites. To prevent metal toxicity, bacteria respond to high intracellular metal concentrations by means of metal-responsive transcriptional regulators that downregulate metal import ...
Between 1996 and 1998, a total of 2,494 samples of blood from humans and animals were collected and tested for brucellosis. This total included 1,594 samples of animal blood, collected from 1,050 sheep from 20 flocks, and 544 goats from eight herds. The serum samples were tested using the Rose Bengal test, the tube agglutination test, the complement fixation test and an enzyme-linked immunosorbent assay. Moreover, a complete history was compiled from each flock/herd. The rate of abortions in sheep due to brucellosis ranged from 0.5% to 56%, with a mean of 33.2%. The goats had a higher abortion rate. Thirty-four aborted sheep foetuses collected from these 20 flocks were bacteriologically and pathologically examined. A pure culture of Brucella melitensis biotype 3 was isolated from 21 of the aborted foetuses.. ...
Authors: JADRANKA ZUTIC, DJORDJE CVETOJEVIC, LJUBISA VELJOVIC, OLIVER RADANOVIC, SLOBODAN STANOJEVIC, BRANISLAV KURELJUSIC Abstract: The objective of this work was to present the first case of Brucella suis biovar 2 isolation in outdoor reared pigs in Serbia. B. suis biovar 2 has not previously been detected in Serbia, from either wild boar or outdoor reared pigs. In our case, brucellosis was discovered in the region of Srem, which geographically constitutes a part of northwestern Serbia. Outdoor reared pigs in the Srem region are part of an extensive breeding system in the field and woods. In the course of a random visit to a herd in the above-mentioned area, the animals owner discovered two aborted fetuses, thereafter presented for laboratory examinations. B. suis biovar 2 was isolated from both fetuses. Isolated strains were identified using both classical and molecular techniques, including genomic sequencing. Based on epizootiological data, we were unable to establish the source of ...
March 24, 2004. Goat milk sold in the United States may soon be better protected against brucellosis-causing bacteria, thanks to recent research conducted by two U.S. Department of Agriculture agencies in Ames, Iowa. A test for detecting the bacteria Brucella melitensis in bulk goat milk has been developed by research chemist Louisa Tabatabai of the Agricultural Research Services National Animal Disease Center (NADC), Barbara Martin of the Animal and Plant Health Inspection Services (APHIS) National Veterinary Services Laboratories, and graduate student Nathan Funk of Iowa State University. The test relies on an adaptation of an enzyme-linked immunoassay (ELISA) that Tabatabai helped develop in 1984 for testing cattle for B. abortus.. B. melitensis, one of six known species of Brucella bacteria that induce abortions in animals, mainly infects sheep and goats. In humans, B. melitensis infection causes Malta fever, which is characterized by fever and headaches.. Few cases of this infection in ...
Background: Brucellosis is a zoonotic disease caused by a Gram negative rod named after Bruce in 1887. It is a zoonosis of worldwide distribution. In Saudi Arabia, the prevalence is high and is attributed to widespread animal husbandry and to the traditional drinking of raw milk. Shoulder brucellosis commonly seen in old population. We have recently treated an old patient diagnosed with brucellosis, who was seen with clinical, radioisotopic, microbiologic, and surgical evidence of shoulder septic arthritis, a rare site of Brucella involvement. Case presentation: A 75 year old male presented with history of fever for the past 10 days with associated right shoulder pain. He was admitted under internal medicine for further investigations for fever of unknown origin. Right shoulder pain was described as generalized dull ache of with gradual onset .Patient Denied any contact with any sheep, animal urine or dairy products, there was no obvious source of ongoing infection and no history of recent ...
Twenty (1.4%) of 1,421 adult Ixodes pacificus ticks and 2 (20%) of 10 adult Ixodes neotomae ticks collected in five counties of northern California were found to contain spirochetes by direct immunofluorescence examination of their tissues with a polyvalent conjugate. Borreliae isolated from the tissues of nine of these ticks (I. pacificus, 8; I. neotomae, 1) were identified as Borrelia burgdorferi with specific monoclonal antibodies and characterized further by polyacrylamide gel electrophoresis and Western blot (immunoblot) analyses. The isolate from I. neotomae was the first to be characterized from a tick other than I. pacificus in western North America. All strains were relatively homogeneous with respect to the kind of OspA proteins they produced, whereas they were heterogeneous with regard to their OspB proteins and to several low-molecular-weight proteins in the 21,500-to-24,000 region. Significant phenotypic variation was observed among isolates obtained within and between populations of I.
Bacillus anthracis is a facultative intracellular bacterial pathogen that can cause cutaneous, gastrointestinal or respiratory disease in many vertebrates, including humans. Commercially available anthrax vaccines for immunization of humans are of limited duration and do not protect against the respiratory form of the disease. Brucella abortus is a facultative intracellular bacterium that causes chronic infection in animals and humans. As with other intracellular pathogens, cell mediated immune responses (CMI) are crucial in affording protection against brucellosis. B. abortus strain RB51 has been shown to be useful in eliciting protective cell mediated immunity and humoral responses against Brucella in cattle and other animal species. Since the protective antigen (PA) of B. anthracis is known to induce protective antibodies, it was decided that the objective of this research was to test whether the gene encoding PA could be expressed in Brucella producing a bivalent vaccine to protect against ...
Prion protein is the causative agent of the transmissible spongiform encephalopathies. According to the "prion hypothesis," the infectious isoform of prion protein, termed PrPSc, replicates by interacting with cellular PrPC and mediating its conformational change into the disease-causing PrPSc (19). Compared with its well-defined pathological significance, the physiological function of PrPC remains unclear. PrPC is highly expressed not only by cells in the CNS but also by follicular DCs, mature myeloid cells, and activated T cells. This distribution suggests involvement of PrPC in immune surveillance (20).. Our present study defines a novel role for PrPC as an M-cell receptor for the uptake of pathogenic bacteria. PrPC on macrophages has been reported to recognize surface-exposed Hsp60 of B. abortus and to facilitate internalization of the bacteria (13); however, Fontes et al. (21) reported a contradictory result. Using Prnp−/− mice, they showed that B. suis infection is independent of PrPC ...

Brucella - WikipediaBrucella - Wikipedia

Brucella abortus A13334, 3,401 ORFs Brucella canis ATCC 23365, 3,408 ORFs Brucella melitensis 16M, 3,279 ORFs Brucella microti ... 3,193 ORFs Brucella pinnipedialis B2/94, 3,505 ORFs Brucella suis 1330, 3,408 ORFs Genome data for these and other Brucella ... Brucella species have been found primarily in mammals: Pathogenic Brucella species can cause abortion in female animals by ... Conversely, depriving Brucella of the blue wavelengths dropped its reproductive rate by 90%. Parte, A.C. "Brucella". Muleme.M ...
more infohttps://en.wikipedia.org/wiki/Brucella

Brucella abortus (                   ...)Brucella abortus ( ...)

Multiporator / Electropo... Making electrocompetent cells: ... Electroporation of cells: Grow ...,Brucella,abortus,biological, ... 4308 915.508 12/2001 Microorganism Brucella abortus Cell type Bacteria, gram negative Molecules injected Plasmid DNA (pBA.sodkn ...
more infohttp://www.bio-medicine.org/biology-technology/Brucella-abortus-582-1/

Humans and Brucella Species  | Clinicians | Brucellosis | CDCHumans and Brucella Species | Clinicians | Brucellosis | CDC

Three types of the bacteria that cause brucellosis - Brucella abortus, Brucella melitensis and Brucella suis - are designated ... Exposure to most species of Brucella, such as those associated with certain types of animals, could potentially lead to ... Human Neurobrucellosis with Intracerebral Granuloma Caused by a Marine Mammal Brucella spp. ...
more infohttps://www.cdc.gov/brucellosis/clinicians/brucella-species.html

BRUCELLA ANTIBODYBRUCELLA ANTIBODY

However, positive results by Brucella serology are not necessarily diagnostic of acute infections, as antibodies may persist ...
more infohttps://www.sgh.com.sg/patient-care/specialties-services/brucella-antibody

Brucella dictionary definition | brucella definedBrucella dictionary definition | brucella defined

bru·cel·lae, or bru·cel·las Any of various aerobic, short, rod-shaped bacteria of the genus Brucella that are pathogenic to ... pl. brucellae (-sĕl′ē) or brucellas Any of various aerobic, short, rod-shaped bacteria of the genus Brucella that are ... brucella. bru·cel·la. noun. pl. bru·cel·lae, or bru·cel·las Any of various aerobic, short, rod-shaped bacteria of the genus ... Brucella f. *A taxonomic genus within the family Brucellaceae "" several gram-negative bacteria that are pathogenic to humans ...
more infohttps://www.yourdictionary.com/brucella

ATP-Binding Cassette Systems of BrucellaATP-Binding Cassette Systems of Brucella

ABC systems of Brucella melitensis 16M, Brucella abortus 9-941, Brucella canis RM6/66, Brucella suis 1330, and Brucella ovis 63 ... Brucella abortus (cattle), Brucella suis (pigs), Brucella ovis (sheep), Brucella canis (dogs), and Brucella neotomae (desert ... Brucella pinnipedialis (seals) [3], Brucella ceti (dolphins and porpoises) [3], and Brucella microti (voles) [4]. Although ... and Brucella pinnipedialis sp. nov. for Brucella strains with cetaceans and seals as their preferred hosts," International ...
more infohttps://www.hindawi.com/journals/ijg/2009/354649/

Human Exposure to Brucella abortus Strain RB51 -- Kansas, 1997Human Exposure to Brucella abortus Strain RB51 -- Kansas, 1997

... On May 26-27, 1997, nine persons (a farmer, four veterinary ... One element of the cooperative state and federal brucellosis eradication efforts is the use of approved Brucella vaccines on ... Editorial Note: Brucellosis, also known as undulant fever or Bangs disease, is a systemic infection caused by Brucella sp ... First, immunologic response to the RB51 strain is not detected on routinely available serologic tests for Brucella. ...
more infohttps://www.cdc.gov/mmWr/preview/mmwrhtml/00051495.htm

Test Detects Brucella Bacteria in Goats Milk : USDA ARSTest Detects Brucella Bacteria in Goat's Milk : USDA ARS

Test Detects Brucella Bacteria in Goats Milk. By Luis Pons. March 24, 2004. Goat milk sold in the United States may soon be ... B. melitensis, one of six known species of Brucella bacteria that induce abortions in animals, mainly infects sheep and goats. ... A test for detecting the bacteria Brucella melitensis in bulk goat milk has been developed by research chemist Louisa Tabatabai ... ARS Home » News & Events » News Articles » Research News » 2004 » Test Detects Brucella Bacteria in Goats Milk ...
more infohttps://www.ars.usda.gov/news-events/news/research-news/2004/test-detects-brucella-bacteria-in-goats-milk/

Brucella Melitensis AntigenBrucella Melitensis Antigen

... SKU: GL-1002 Retail Price: $24.50. Our LOW Price: $18.33. You Save: $6.17 (25%). Availability: ... Home » Medical Equipment & Supplies » Instruments & Disposables » Urine Reagent Strips, Individual Packs » Brucella Melitensis ...
more infohttp://www.egeneralmedical.com/gl-1002-brucella-melitensis-antigen.html

Brucella speciesBrucella species

Brucella spp. are concentrated in the genital tract and milk of infected animals. B. melitensis infects primarily goats, sheep ... Whenever cultivation of Brucella spp. from blood, bone marrow, or other tissues is attempted, it is essential to notify the ... Brucella spp. enter hosts via ingestion, inhalation, or inoculation through mucous membranes or non-intact skin. The bacteria ... Rifampin should be omitted if the exposure is to Brucella abortus strain RB51, which is resistant to rifampin. For accidental ...
more infohttps://www.psychiatryadvisor.com/infectious-diseases/brucella-species/article/610445/

KEGG PATHWAY: Biotin metabolism - Brucella ovisKEGG PATHWAY: Biotin metabolism - Brucella ovis

Biotin metabolism - Brucella ovis [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show description , User data ...
more infohttp://www.genome.jp/kegg-bin/show_pathway?bov00780

View source for Brucella - microbewikiView source for Brucella - microbewiki

Brucella abortus, Brucella canis, Brucella cetaceae, Brucella maris , Brucella melitensis, Brucella pinnipediae, Brucella sp ... Completion of the genome sequence of Brucella abortus and comparison to the highly similar genomes of Brucella melitensis and ... Brucella enters and lives in such different host cells such as epithelial cells are not known. Brucella is generally ... He started anti-brucella therapy, which continued for 1 year and the enhancement and edema receded (Sohn et al. 2003). == ...
more infohttps://microbewiki.kenyon.edu/index.php?title=Brucella&action=edit

Can brucella cause brain problems? | Feature | Pulse TodayCan brucella cause 'brain problems'? | Feature | Pulse Today

Complaints of short-term memory loss with no cognitive deficit were common in Gulf War veterans, in whom brucella screening ... Proposed as a cause of chronic fatigue syndrome, we receive regular requests for brucella serology after a patient has visited ... A - Now almost exclusively acquired abroad, brucella is an unlikely cause of his symptoms. An intracellular parasite that is ... due to a reservoir of brucellae in tissues including lymphatics, bones and liver. ...
more infohttp://www.pulsetoday.co.uk/can-brucella-cause-brain-problems/10881106.article

Brucella lipopolysaccharide acts as a virulence factor.  - PubMed - NCBIBrucella lipopolysaccharide acts as a virulence factor. - PubMed - NCBI

Brucella is a facultative intracellular bacterium responsible for brucellosis. Virulence factors involved in Brucella ... Brucella lipopolysaccharide acts as a virulence factor.. Lapaque N1, Moriyon I, Moreno E, Gorvel JP. ... These properties make lipopolysaccharide an important virulence factor for Brucella survival and replication in the host. ... bacterial outer membrane components have also been described as being implicated in Brucella survival in the host. For example ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/15694858?dopt=Abstract

Glucose Transport in Brucella abortus | Journal of BacteriologyGlucose Transport in Brucella abortus | Journal of Bacteriology

Thank you for sharing this Journal of Bacteriology article.. NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.. ...
more infohttps://jb.asm.org/content/118/1/250/article-info

Brucella abortus IgA ELISA Kit (CE)Brucella abortus IgA ELISA Kit (CE)

This assay utilizes antigen from Brucella abortus strain W99. ... The Brucella abortus IgA ELISA Kit is intended for the ... measurement of human IgA antibodies to Brucella abortus. ... Brucella abortus IgA ELISA Kit (CE). Packaging size: 96 Wells ... The Brucella abortus ELISA Kit is intended for the measurement of human IgG, IgA, or IgM antibodies to Brucella abortus. This ... The Brucella abortus IgA ELISA Kit is intended for the measurement of human IgA antibodies to Brucella abortus. This assay ...
more infohttps://www.mpbio.com/07i526102-brucella-abortus-iga-elisa-kit

KEGG PATHWAY: Sulfur metabolism - Brucella melitensis M28KEGG PATHWAY: Sulfur metabolism - Brucella melitensis M28

Sulfur metabolism - Brucella melitensis M28 [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show description ...
more infohttp://www.genome.jp/kegg-bin/show_pathway?bmz00920

Brucella endocarditis caused by brucella melitensisBrucella endocarditis caused by brucella melitensis

We present a rare case of brucella endocarditis, forming a vegetation on the mitral valve. The definitive diagnosis has been ... demonstration of the vegetation with the echocardiography and with the production from the multiple blood culture of brucella ...
more infohttp://connection.ebscohost.com/c/case-studies/101634984/brucella-endocarditis-caused-by-brucella-melitensis

An Unusual Case of Brucella abortus Prosthetic Joint Infection.  - PubMed - NCBIAn Unusual Case of Brucella abortus Prosthetic Joint Infection. - PubMed - NCBI

Brucellosis is a systemic infection caused by brucella species. Prosthetic joint infection due to brucella species is rare. We ... An Unusual Case of Brucella abortus Prosthetic Joint Infection.. Walsh J1, Gilleece A2, Fenelon L1, Cogley D3, Schaffer K1. ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/31966957

Brucella | definition of Brucella by Medical dictionaryBrucella | definition of Brucella by Medical dictionary

Brucella explanation free. What is Brucella? Meaning of Brucella medical term. What does Brucella mean? ... Looking for online definition of Brucella in the Medical Dictionary? ... Related to Brucella: Francisella, Brucella abortus, Brucella canis, Brucella melitensis Brucella. [broo-sel´ah] a genus of gram ... brucella. /bru·cel·la/ (broo-sel´ah) any member of the genus Brucella. .brucel´lar. brucella. (bro͞o-sĕl′ə). n. pl. bru·cellae ...
more infohttp://medical-dictionary.thefreedictionary.com/Brucella

Brucella Septic Arthritis: Case Reports and Review of the LiteratureBrucella Septic Arthritis: Case Reports and Review of the Literature

Brucella serology done by immunocapture assay was significantly raised 1 : 10240. Blood culture isolated Brucella species but ... Synovial cultures isolated Brucella species but blood culture was negative. Brucella serology in the blood was high; it was 1 ... M. I. Khateeb, G. F. Araj, S. A. Majeed, and A. R. Lulu, "Brucella arthritis: a study of 96 cases in Kuwait," Annals of the ... The blood urea and creatinine was raised at 16.8 mmol/L and 140 μmol/L. Synovial fluid cultures were positive for Brucella ...
more infohttps://www.hindawi.com/journals/criid/2016/4687840/

Metals and the Biology and Virulence of Brucella | SpringerLinkMetals and the Biology and Virulence of Brucella | SpringerLink

Brucella strains microbiology Iron enzyme dependence Manganese enzymes bacteria Zn brucella strain Biometals ... Researchers and professionals studying zoonotic pathogens from the genus Brucella find this book valuable. ... The Role of Zinc in the Biology and Virulence of Brucella Strains ... in the basic biology and virulence of bacterial pathogens from the genus Brucella. The authors explore the biological functions ...
more infohttps://link.springer.com/book/10.1007%2F978-3-319-53622-4

Brucella melitensis | definition of Brucella melitensis by Medical dictionaryBrucella melitensis | definition of Brucella melitensis by Medical dictionary

What is Brucella melitensis? Meaning of Brucella melitensis medical term. What does Brucella melitensis mean? ... Looking for online definition of Brucella melitensis in the Medical Dictionary? Brucella melitensis explanation free. ... Related to Brucella melitensis: Brucella canis, Brucella abortus, Brucella suis. Bru·cel·la me·li·ten·sis. (brū-selă mel-i- ... See also: Brucella. Brucella. a genus of gram-negative rods in seven species and several biotypes. The cause of many serious ...
more infohttp://medical-dictionary.thefreedictionary.com/Brucella+melitensis

Brucella BvrR protein
     Summary Report | CureHunterBrucella BvrR protein Summary Report | CureHunter

BvrR/BvrS represent the two-component system involved in the regulation of Brucella virulence; amino acid sequence in first ... Brucella BvrR protein. Subscribe to New Research on Brucella BvrR protein BvrR/BvrS represent the two-component system involved ... in the regulation of Brucella virulence; amino acid sequence in first source ...
more infohttp://www.curehunter.com/public/keywordSummaryC471339-Brucella-BvrR-protein.do

Frontiers | Lipopolysaccharide-Deficient Brucella Variants Arise Spontaneously during Infection | MicrobiologyFrontiers | Lipopolysaccharide-Deficient Brucella Variants Arise Spontaneously during Infection | Microbiology

The experiments reported describe the direct recovery of Brucella from macrophages infected in vitro and from the spleens of ... The experiments reported describe the direct recovery of Brucella from macrophages infected in vitro and from the spleens of ... However, in situ analysis of Brucella infected macrophages using antibody directed against O-polysaccharide suggested a loss of ... However, in situ analysis of Brucella infected macrophages using antibody directed against O-polysaccharide suggested a loss of ...
more infohttps://www.frontiersin.org/articles/10.3389/fmicb.2011.00054/full
  • Brucella lipopolysaccharide acts as a virulence factor. (nih.gov)
  • Virulence factors involved in Brucella replication and Brucella's strategies to circumvent the immune response are under investigation. (nih.gov)
  • These properties make lipopolysaccharide an important virulence factor for Brucella survival and replication in the host. (nih.gov)
  • On May 26-27, 1997, nine persons (a farmer, four veterinary clinicians, and four veterinary students) in Manhattan, Kansas, participated in an attempted vaginal delivery, a cesarean delivery, and a necropsy on a stillborn calf that died because of Brucella abortus infection. (cdc.gov)
  • After exposure to Brucella, humans generally have a two- to four-week latency period before exhibiting symptoms, which include acute undulating fever (>90% of all cases), headache, arthralgia (>50%), night sweats, fatigue, and anorexia. (wikipedia.org)
  • Transmission to humans takes place via three recognised channels: (i) the consumption of infected animal products, (ii) direct contact with infected animal birth products, and (iii) the inhalation of aerosolised Brucella . (hindawi.com)
  • None of the exposed persons reported having previously had brucellosis or being unintentionally inoculated with Brucella vaccine. (cdc.gov)
  • Its very unlikely that there will be an expert on Brucella (in pigs) reading this. (protocol-online.org)
  • What I would suggest you to do is to look up Brucella experts that have experience with pigs as well. (protocol-online.org)
  • brucellosis - an infectious disease caused by Brucella, and transmitted by direct contact with diseased animals or through ingestion of infected meat, milk, or cheese. (thefreedictionary.com)
  • Most of the data is based on sero-epidemiological studies although it is generally accepted that only the isolation of Brucella confirms the presence of brucellosis. (au-ibar.org)
  • Garrity, George M. "Nomenclature Abstract for Brucella papionis Whatmore et al. (wikipedia.org)
  • Brucella can also target the male reproductive tract. (wikipedia.org)
  • In addition to this secretion system, bacterial outer membrane components have also been described as being implicated in Brucella survival in the host. (nih.gov)
  • Brucella is the cause of brucellosis, which is a zoonosis transmitted by ingesting contaminated food (such as unpasteurized milk products), direct contact with an infected animal, or inhalation of aerosols. (wikipedia.org)