Washing liquid obtained from irrigation of the lung, including the BRONCHI and the PULMONARY ALVEOLI. It is generally used to assess biochemical, inflammatory, or infection status of the lung.
Washing out of the lungs with saline or mucolytic agents for diagnostic or therapeutic purposes. It is very useful in the diagnosis of diffuse pulmonary infiltrates in immunosuppressed patients.
Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.
Endoscopic examination, therapy or surgery of the bronchi.
The washing of a body cavity or surface by flowing water or solution for therapy or diagnosis.
Round, granular, mononuclear phagocytes found in the alveoli of the lungs. They ingest small inhaled particles resulting in degradation and presentation of the antigen to immunocompetent cells.
Sarcoidosis affecting predominantly the lungs, the site most frequently involved and most commonly causing morbidity and mortality in sarcoidosis. Pulmonary sarcoidosis is characterized by sharply circumscribed granulomas in the alveolar, bronchial, and vascular walls, composed of tightly packed cells derived from the mononuclear phagocyte system. The clinical symptoms when present are dyspnea upon exertion, nonproductive cough, and wheezing. (Cecil Textbook of Medicine, 19th ed, p431)
Infection of the lung often accompanied by inflammation.
A process in which normal lung tissues are progressively replaced by FIBROBLASTS and COLLAGEN causing an irreversible loss of the ability to transfer oxygen into the bloodstream via PULMONARY ALVEOLI. Patients show progressive DYSPNEA finally resulting in death.
Small polyhedral outpouchings along the walls of the alveolar sacs, alveolar ducts and terminal bronchioles through the walls of which gas exchange between alveolar air and pulmonary capillary blood takes place.
Pathological processes involving any part of the LUNG.
An idiopathic systemic inflammatory granulomatous disorder comprised of epithelioid and multinucleated giant cells with little necrosis. It usually invades the lungs with fibrosis and may also involve lymph nodes, skin, liver, spleen, eyes, phalangeal bones, and parotid glands.
Medical procedure involving the emptying of contents in the stomach through the use of a tube inserted through the nose or mouth. It is performed to remove poisons or relieve pressure due to intestinal blockages or during surgery.
A common interstitial lung disease caused by hypersensitivity reactions of PULMONARY ALVEOLI after inhalation of and sensitization to environmental antigens of microbial, animal, or chemical sources. The disease is characterized by lymphocytic alveolitis and granulomatous pneumonitis.
A condition characterized by infiltration of the lung with EOSINOPHILS due to inflammation or other disease processes. Major eosinophilic lung diseases are the eosinophilic pneumonias caused by infections, allergens, or toxic agents.
Tendency of the smooth muscle of the tracheobronchial tree to contract more intensely in response to a given stimulus than it does in the response seen in normal individuals. This condition is present in virtually all symptomatic patients with asthma. The most prominent manifestation of this smooth muscle contraction is a decrease in airway caliber that can be readily measured in the pulmonary function laboratory.
Granular leukocytes with a nucleus that usually has two lobes connected by a slender thread of chromatin, and cytoplasm containing coarse, round granules that are uniform in size and stainable by eosin.
Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.
An albumin obtained from the white of eggs. It is a member of the serpin superfamily.
A pulmonary disease in humans occurring in immunodeficient or malnourished patients or infants, characterized by DYSPNEA, tachypnea, and HYPOXEMIA. Pneumocystis pneumonia is a frequently seen opportunistic infection in AIDS. It is caused by the fungus PNEUMOCYSTIS JIROVECII. The disease is also found in other MAMMALS where it is caused by related species of Pneumocystis.
The larger air passages of the lungs arising from the terminal bifurcation of the TRACHEA. They include the largest two primary bronchi which branch out into secondary bronchi, and tertiary bronchi which extend into BRONCHIOLES and PULMONARY ALVEOLI.
A form of bronchial disorder with three distinct components: airway hyper-responsiveness (RESPIRATORY HYPERSENSITIVITY), airway INFLAMMATION, and intermittent AIRWAY OBSTRUCTION. It is characterized by spasmodic contraction of airway smooth muscle, WHEEZING, and dyspnea (DYSPNEA, PAROXYSMAL).
The number of CELLS of a specific kind, usually measured per unit volume or area of sample.
The number of WHITE BLOOD CELLS per unit volume in venous BLOOD. A differential leukocyte count measures the relative numbers of the different types of white cells.
Washing out of the peritoneal cavity. The procedure is a diagnostic as well as a therapeutic technique following abdominal trauma or inflammation.
A form of hypersensitivity affecting the respiratory tract. It includes ASTHMA and RHINITIS, ALLERGIC, SEASONAL.
Damage to any compartment of the lung caused by physical, chemical, or biological agents which characteristically elicit inflammatory reaction. These inflammatory reactions can either be acute and dominated by NEUTROPHILS, or chronic and dominated by LYMPHOCYTES and MACROPHAGES.
A diverse group of lung diseases that affect the lung parenchyma. They are characterized by an initial inflammation of PULMONARY ALVEOLI that extends to the interstitium and beyond leading to diffuse PULMONARY FIBROSIS. Interstitial lung diseases are classified by their etiology (known or unknown causes), and radiological-pathological features.
Antigen-type substances that produce immediate hypersensitivity (HYPERSENSITIVITY, IMMEDIATE).
Fluid obtained by THERAPEUTIC IRRIGATION or washout of the nasal cavity and NASAL MUCOSA. The resulting fluid is used in cytologic and immunologic assays of the nasal mucosa such as with the NASAL PROVOCATION TEST in the diagnosis of nasal hypersensitivity.
A condition of lung damage that is characterized by bilateral pulmonary infiltrates (PULMONARY EDEMA) rich in NEUTROPHILS, and in the absence of clinical HEART FAILURE. This can represent a spectrum of pulmonary lesions, endothelial and epithelial, due to numerous factors (physical, chemical, or biological).
A PULMONARY ALVEOLI-filling disease, characterized by dense phospholipoproteinaceous deposits in the alveoli, cough, and DYSPNEA. This disease is often related to, congenital or acquired, impaired processing of PULMONARY SURFACTANTS by alveolar macrophages, a process dependent on GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR.
A syndrome characterized by progressive life-threatening RESPIRATORY INSUFFICIENCY in the absence of known LUNG DISEASES, usually following a systemic insult such as surgery or major TRAUMA.
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
A genus of ascomycetous FUNGI, family Pneumocystidaceae, order Pneumocystidales. It includes various host-specific species causing PNEUMOCYSTIS PNEUMONIA in humans and other MAMMALS.
Inflammation of the lung parenchyma that is caused by bacterial infections.
A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function.
Substances and drugs that lower the SURFACE TENSION of the mucoid layer lining the PULMONARY ALVEOLI.
An abundant pulmonary surfactant-associated protein that binds to a variety of lung pathogens and enhances their opsinization and killing by phagocytic cells. Surfactant protein D contains a N-terminal collagen-like domain and a C-terminal lectin domain that are characteristic of members of the collectin family of proteins.
Material coughed up from the lungs and expectorated via the mouth. It contains MUCUS, cellular debris, and microorganisms. It may also contain blood or pus.
An abundant pulmonary surfactant-associated protein that binds to a variety of lung pathogens, resulting in their opsinization. It also stimulates MACROPHAGES to undergo PHAGOCYTOSIS of microorganisms. Surfactant protein A contains a N-terminal collagen-like domain and a C-terminal lectin domain that are characteristic of members of the collectin family of proteins.
The mucous membrane lining the RESPIRATORY TRACT, including the NASAL CAVITY; the LARYNX; the TRACHEA; and the BRONCHI tree. The respiratory mucosa consists of various types of epithelial cells ranging from ciliated columnar to simple squamous, mucous GOBLET CELLS, and glands containing both mucous and serous cells.
The administration of drugs by the respiratory route. It includes insufflation into the respiratory tract.
Measurement of the various processes involved in the act of respiration: inspiration, expiration, oxygen and carbon dioxide exchange, lung volume and compliance, etc.
Excessive accumulation of extravascular fluid in the lung, an indication of a serious underlying disease or disorder. Pulmonary edema prevents efficient PULMONARY GAS EXCHANGE in the PULMONARY ALVEOLI, and can be life-threatening.
An enzyme that catalyzes the hydrolysis of proteins, including elastin. It cleaves preferentially bonds at the carboxyl side of Ala and Val, with greater specificity for Ala. EC
An immunoglobulin associated with MAST CELLS. Overexpression has been associated with allergic hypersensitivity (HYPERSENSITIVITY, IMMEDIATE).
Colloids with a gaseous dispersing phase and either liquid (fog) or solid (smoke) dispersed phase; used in fumigation or in inhalation therapy; may contain propellant agents.
Tests involving inhalation of allergens (nebulized or in dust form), nebulized pharmacologically active solutions (e.g., histamine, methacholine), or control solutions, followed by assessment of respiratory function. These tests are used in the diagnosis of asthma.
Ratio of T-LYMPHOCYTES that express the CD4 ANTIGEN to those that express the CD8 ANTIGEN. This value is commonly assessed in the diagnosis and staging of diseases affecting the IMMUNE SYSTEM including HIV INFECTIONS.
The transference of either one or both of the lungs from one human or animal to another.
Lung infections with the invasive forms of ASPERGILLUS, usually after surgery, transplantation, prolonged NEUTROPENIA or treatment with high-doses of CORTICOSTEROIDS. Invasive pulmonary aspergillosis can progress to CHRONIC NECROTIZING PULMONARY ASPERGILLOSIS or hematogenous spread to other organs.
The unstable triatomic form of oxygen, O3. It is a powerful oxidant that is produced for various chemical and industrial uses. Its production is also catalyzed in the ATMOSPHERE by ULTRAVIOLET RAY irradiation of oxygen or other ozone precursors such as VOLATILE ORGANIC COMPOUNDS and NITROGEN OXIDES. About 90% of the ozone in the atmosphere exists in the stratosphere (STRATOSPHERIC OZONE).
Inflammation of the lung parenchyma that is caused by a viral infection.
The diffusion or accumulation of neutrophils in tissues or cells in response to a wide variety of substances released at the sites of inflammatory reactions.
Quartz (SiO2). A glassy or crystalline form of silicon dioxide. Many colored varieties are semiprecious stones. (From Grant & Hackh's Chemical Dictionary, 5th ed)
A cytokine that promotes differentiation and activation of EOSINOPHILS. It also triggers activated B-LYMPHOCYTES to differentiate into IMMUNOGLOBULIN-secreting cells.
Pulmonary diseases caused by fungal infections, usually through hematogenous spread.
A quaternary ammonium parasympathomimetic agent with the muscarinic actions of ACETYLCHOLINE. It is hydrolyzed by ACETYLCHOLINESTERASE at a considerably slower rate than ACETYLCHOLINE and is more resistant to hydrolysis by nonspecific CHOLINESTERASES so that its actions are more prolonged. It is used as a parasympathomimetic bronchoconstrictor agent and as a diagnostic aid for bronchial asthma. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1116)
The tubular and cavernous organs and structures, by means of which pulmonary ventilation and gas exchange between ambient air and the blood are brought about.
Any method of artificial breathing that employs mechanical or non-mechanical means to force the air into and out of the lungs. Artificial respiration or ventilation is used in individuals who have stopped breathing or have RESPIRATORY INSUFFICIENCY to increase their intake of oxygen (O2) and excretion of carbon dioxide (CO2).
A complex of related glycopeptide antibiotics from Streptomyces verticillus consisting of bleomycin A2 and B2. It inhibits DNA metabolism and is used as an antineoplastic, especially for solid tumors.
A CXC chemokine that is synthesized by activated MONOCYTES and NEUTROPHILS. It has specificity for CXCR2 RECEPTORS.
The exposure to potentially harmful chemical, physical, or biological agents by inhaling them.
Altered reactivity to an antigen, which can result in pathologic reactions upon subsequent exposure to that particular antigen.
Compounds that accept electrons in an oxidation-reduction reaction. The reaction is induced by or accelerated by exposure to electromagnetic radiation in the spectrum of visible or ultraviolet light.
Water-soluble proteins found in egg whites, blood, lymph, and other tissues and fluids. They coagulate upon heating.
Proteins found in the LUNG that act as PULMONARY SURFACTANTS.
Lipid-containing polysaccharides which are endotoxins and important group-specific antigens. They are often derived from the cell wall of gram-negative bacteria and induce immunoglobulin secretion. The lipopolysaccharide molecule consists of three parts: LIPID A, core polysaccharide, and O-specific chains (O ANTIGENS). When derived from Escherichia coli, lipopolysaccharides serve as polyclonal B-cell mitogens commonly used in laboratory immunology. (From Dorland, 28th ed)
Endoscopes for the visualization of the interior of the bronchi.
Abnormal increase of EOSINOPHILS in the blood, tissues or organs.
Pneumonia due to aspiration or inhalation of various oily or fatty substances.
A human or animal whose immunologic mechanism is deficient because of an immunodeficiency disorder or other disease or as the result of the administration of immunosuppressive drugs or radiation.
Inflammation of the large airways in the lung including any part of the BRONCHI, from the PRIMARY BRONCHI to the TERTIARY BRONCHI.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
A member of the CXC chemokine family that plays a role in the regulation of the acute inflammatory response. It is secreted by variety of cell types and induces CHEMOTAXIS of NEUTROPHILS and other inflammatory cells.
A form of pneumoconiosis caused by inhalation of asbestos fibers which elicit potent inflammatory responses in the parenchyma of the lung. The disease is characterized by interstitial fibrosis of the lung, varying from scattered sites to extensive scarring of the alveolar interstitium.
Delivery of medications through the nasal mucosa.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
Class of pro-inflammatory cytokines that have the ability to attract and activate leukocytes. They can be divided into at least three structural branches: C; (CHEMOKINES, C); CC; (CHEMOKINES, CC); and CXC; (CHEMOKINES, CXC); according to variations in a shared cysteine motif.
The administration of therapeutic agents drop by drop, as eye drops, ear drops, or nose drops. It is also administered into a body space or cavity through a catheter. It differs from THERAPEUTIC IRRIGATION in that the irrigate is removed within minutes, but the instillate is left in place.
Inflammation of the BRONCHIOLES.
Serious INFLAMMATION of the LUNG in patients who required the use of PULMONARY VENTILATOR. It is usually caused by cross bacterial infections in hospitals (NOSOCOMIAL INFECTIONS).
A type of lung inflammation resulting from the aspiration of food, liquid, or gastric contents into the upper RESPIRATORY TRACT.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
A hemeprotein from leukocytes. Deficiency of this enzyme leads to a hereditary disorder coupled with disseminated moniliasis. It catalyzes the conversion of a donor and peroxide to an oxidized donor and water. EC
Infections of the lungs with parasites, most commonly by parasitic worms (HELMINTHS).
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
The cartilaginous and membranous tube descending from the larynx and branching into the right and left main bronchi.
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.
The viscous secretion of mucous membranes. It contains mucin, white blood cells, water, inorganic salts, and exfoliated cells.
Inflammation of the BRONCHIOLES leading to an obstructive lung disease. Bronchioles are characterized by fibrous granulation tissue with bronchial exudates in the lumens. Clinical features include a nonproductive cough and DYSPNEA.
A species of PNEUMOCYSTIS infecting humans and causing PNEUMOCYSTIS PNEUMONIA. It also occasionally causes extrapulmonary disease in immunocompromised patients. Its former name was Pneumocystis carinii f. sp. hominis.
A form of alveolitis or pneumonitis due to an acquired hypersensitivity to inhaled antigens associated with farm environment. Antigens in the farm dust are commonly from bacteria actinomycetes (SACCHAROPOLYSPORA and THERMOACTINOMYCES), fungi, and animal proteins in the soil, straw, crops, pelts, serum, and excreta.
Inflammation of the lung parenchyma that is associated with BRONCHITIS, usually involving lobular areas from TERMINAL BRONCHIOLES to the PULMONARY ALVEOLI. The affected areas become filled with exudate that forms consolidated patches.
Narrowing of the caliber of the BRONCHI, physiologically or as a result of pharmacological intervention.
The endogenous compounds that mediate inflammation (AUTACOIDS) and related exogenous compounds including the synthetic prostaglandins (PROSTAGLANDINS, SYNTHETIC).
Earth or other matter in fine, dry particles. (Random House Unabridged Dictionary, 2d ed)
An interstitial lung disease of unknown etiology, occurring between 21-80 years of age. It is characterized by a dramatic onset of a "pneumonia-like" illness with cough, fever, malaise, fatigue, and weight loss. Pathological features include prominent interstitial inflammation without collagen fibrosis, diffuse fibroblastic foci, and no microscopic honeycomb change. There is excessive proliferation of granulation tissue within small airways and alveolar ducts.
Lung damage that is caused by the adverse effects of PULMONARY VENTILATOR usage. The high frequency and tidal volumes produced by a mechanical ventilator can cause alveolar disruption and PULMONARY EDEMA.
Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
A glandular epithelial cell or a unicellular gland. Goblet cells secrete MUCUS. They are scattered in the epithelial linings of many organs, especially the SMALL INTESTINE and the RESPIRATORY TRACT.
Elements of limited time intervals, contributing to particular results or situations.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
A steroid-inducible protein that was originally identified in uterine fluid. It is a secreted homodimeric protein with identical 70-amino acid subunits that are joined in an antiparallel orientation by two disulfide bridges. A variety of activities are associated with uteroglobin including the sequestering of hydrophobic ligands and the inhibition of SECRETORY PHOSPHOLIPASE A2.
The structural changes in the number, mass, size and/or composition of the airway tissues.
Protein-lipid combinations abundant in brain tissue, but also present in a wide variety of animal and plant tissues. In contrast to lipoproteins, they are insoluble in water, but soluble in a chloroform-methanol mixture. The protein moiety has a high content of hydrophobic amino acids. The associated lipids consist of a mixture of GLYCEROPHOSPHATES; CEREBROSIDES; and SULFOGLYCOSPHINGOLIPIDS; while lipoproteins contain PHOSPHOLIPIDS; CHOLESTEROL; and TRIGLYCERIDES.
Subset of helper-inducer T-lymphocytes which synthesize and secrete the interleukins IL-4, IL-5, IL-6, and IL-10. These cytokines influence B-cell development and antibody production as well as augmenting humoral responses.
Enlargement of air spaces distal to the TERMINAL BRONCHIOLES where gas-exchange normally takes place. This is usually due to destruction of the alveolar wall. Pulmonary emphysema can be classified by the location and distribution of the lesions.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Mechanical devices used to produce or assist pulmonary ventilation.
A secreted matrix metalloproteinase which is highly expressed by MACROPHAGES where it may play a role in INFLAMMATION and WOUND HEALING.
Infections with fungi of the genus ASPERGILLUS.
Inhaling liquid or solids, such as stomach contents, into the RESPIRATORY TRACT. When this causes severe lung damage, it is called ASPIRATION PNEUMONIA.
A common interstitial lung disease of unknown etiology, usually occurring between 50-70 years of age. Clinically, it is characterized by an insidious onset of breathlessness with exertion and a nonproductive cough, leading to progressive DYSPNEA. Pathological features show scant interstitial inflammation, patchy collagen fibrosis, prominent fibroblast proliferation foci, and microscopic honeycomb change.
The barrier between capillary blood and alveolar air comprising the alveolar EPITHELIUM and capillary ENDOTHELIUM with their adherent BASEMENT MEMBRANE and EPITHELIAL CELL cytoplasm. PULMONARY GAS EXCHANGE occurs across this membrane.
A cytokine synthesized by T-LYMPHOCYTES that produces proliferation, immunoglobulin isotype switching, and immunoglobulin production by immature B-LYMPHOCYTES. It appears to play a role in regulating inflammatory and immune responses.
X-ray visualization of the chest and organs of the thoracic cavity. It is not restricted to visualization of the lungs.
An abnormal increase in the amount of oxygen in the tissues and organs.
Liquid components of living organisms.
A protease of broad specificity, obtained from dried pancreas. Molecular weight is approximately 25,000. The enzyme breaks down elastin, the specific protein of elastic fibers, and digests other proteins such as fibrin, hemoglobin, and albumin. EC
Physiologically, the opposition to flow of air caused by the forces of friction. As a part of pulmonary function testing, it is the ratio of driving pressure to the rate of air flow.
Inhaling and exhaling the smoke of burning TOBACCO.
Removal and pathologic examination of specimens in the form of small pieces of tissue from the living body.
Polysaccharides consisting of mannose units.
The capability of the LUNGS to distend under pressure as measured by pulmonary volume change per unit pressure change. While not a complete description of the pressure-volume properties of the lung, it is nevertheless useful in practice as a measure of the comparative stiffness of the lung. (From Best & Taylor's Physiological Basis of Medical Practice, 12th ed, p562)
Substances that reduce or suppress INFLAMMATION.
White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES).
A class of statistical methods applicable to a large set of probability distributions used to test for correlation, location, independence, etc. In most nonparametric statistical tests, the original scores or observations are replaced by another variable containing less information. An important class of nonparametric tests employs the ordinal properties of the data. Another class of tests uses information about whether an observation is above or below some fixed value such as the median, and a third class is based on the frequency of the occurrence of runs in the data. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed, p1284; Corsini, Concise Encyclopedia of Psychology, 1987, p764-5)
The technology of transmitting light over long distances through strands of glass or other transparent material.
The property of blood capillary ENDOTHELIUM that allows for the selective exchange of substances between the blood and surrounding tissues and through membranous barriers such as the BLOOD-AIR BARRIER; BLOOD-AQUEOUS BARRIER; BLOOD-BRAIN BARRIER; BLOOD-NERVE BARRIER; BLOOD-RETINAL BARRIER; and BLOOD-TESTIS BARRIER. Small lipid-soluble molecules such as carbon dioxide and oxygen move freely by diffusion. Water and water-soluble molecules cannot pass through the endothelial walls and are dependent on microscopic pores. These pores show narrow areas (TIGHT JUNCTIONS) which may limit large molecule movement.
Pulmonary injury following the breathing in of toxic smoke from burning materials such as plastics, synthetics, building materials, etc. This injury is the most frequent cause of death in burn patients.
A genus of mitosporic fungi containing about 100 species and eleven different teleomorphs in the family Trichocomaceae.
Infections with bacteria of the genus PSEUDOMONAS.
Transparent, tasteless crystals found in nature as agate, amethyst, chalcedony, cristobalite, flint, sand, QUARTZ, and tridymite. The compound is insoluble in water or acids except hydrofluoric acid.
Agents causing the narrowing of the lumen of a bronchus or bronchiole.
A form of alveolitis or pneumonitis due to an acquired hypersensitivity to inhaled avian antigens, usually proteins in the dust of bird feathers and droppings.
An infection caused by an organism which becomes pathogenic under certain conditions, e.g., during immunosuppression.
A 66-kDa peroxidase found in EOSINOPHIL granules. Eosinophil peroxidase is a cationic protein with a pI of 10.8 and is comprised of a heavy chain subunit and a light chain subunit. It possesses cytotoxic activity towards BACTERIA and other organisms, which is attributed to its peroxidase activity.
A mixture of liquid hydrocarbons obtained from petroleum. It is used as laxative, lubricant, ointment base, and emollient.
Water content outside of the lung vasculature. About 80% of a normal lung is made up of water, including intracellular, interstitial, and blood water. Failure to maintain the normal homeostatic fluid exchange between the vascular space and the interstitium of the lungs can result in PULMONARY EDEMA and flooding of the alveolar space.
The movement of leukocytes in response to a chemical concentration gradient or to products formed in an immunologic reaction.
Proteins found in EOSINOPHIL granules. They are primarily basic proteins that play a role in host defense and the proinflammatory actions of activated eosinophils.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
An autosomal recessive genetic disease of the EXOCRINE GLANDS. It is caused by mutations in the gene encoding the CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR expressed in several organs including the LUNG, the PANCREAS, the BILIARY SYSTEM, and the SWEAT GLANDS. Cystic fibrosis is characterized by epithelial secretory dysfunction associated with ductal obstruction resulting in AIRWAY OBSTRUCTION; chronic RESPIRATORY INFECTIONS; PANCREATIC INSUFFICIENCY; maldigestion; salt depletion; and HEAT PROSTRATION.
Animals or humans raised in the absence of a particular disease-causing virus or other microorganism. Less frequently plants are cultivated pathogen-free.
A procedure involving placement of a tube into the trachea through the mouth or nose in order to provide a patient with oxygen and anesthesia.
MYCOBACTERIUM infections of the lung.
A highly toxic gas that has been used as a chemical warfare agent. It is an insidious poison as it is not irritating immediately, even when fatal concentrations are inhaled. (From The Merck Index, 11th ed, p7304)
The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.
A cytokine that stimulates the growth and differentiation of B-LYMPHOCYTES and is also a growth factor for HYBRIDOMAS and plasmacytomas. It is produced by many different cells including T-LYMPHOCYTES; MONOCYTES; and FIBROBLASTS.
Diseases of domestic and wild horses of the species Equus caballus.
A hydroxylated form of the imino acid proline. A deficiency in ASCORBIC ACID can result in impaired hydroxyproline formation.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Infections of the respiratory tract with fungi of the genus ASPERGILLUS. Infections may result in allergic reaction (ALLERGIC BRONCHOPULMONARY ASPERGILLOSIS), colonization in pulmonary cavities as fungus balls (MYCETOMA), or lead to invasion of the lung parenchyma (INVASIVE PULMONARY ASPERGILLOSIS).
A form of pneumoconiosis resulting from inhalation of dust containing crystalline form of SILICON DIOXIDE, usually in the form of quartz. Amorphous silica is relatively nontoxic.
A metabolite of BROMHEXINE that stimulates mucociliary action and clears the air passages in the respiratory tract. It is usually administered as the hydrochloride.
Excess of normal lymphocytes in the blood or in any effusion.
Invasion of the host RESPIRATORY SYSTEM by microorganisms, usually leading to pathological processes or diseases.
The major metabolite in neutrophil polymorphonuclear leukocytes. It stimulates polymorphonuclear cell function (degranulation, formation of oxygen-centered free radicals, arachidonic acid release, and metabolism). (From Dictionary of Prostaglandins and Related Compounds, 1990)
A pulmonary surfactant associated-protein that plays an essential role in alveolar stability by lowering the surface tension at the air-liquid interface. Inherited deficiency of pulmonary surfactant-associated protein B is one cause of RESPIRATORY DISTRESS SYNDROME, NEWBORN.
A CC-type chemokine that is specific for CCR3 RECEPTORS. It is a potent chemoattractant for EOSINOPHILS.
A soluble factor produced by activated T-LYMPHOCYTES that induces the expression of MHC CLASS II GENES and FC RECEPTORS on B-LYMPHOCYTES and causes their proliferation and differentiation. It also acts on T-lymphocytes, MAST CELLS, and several other hematopoietic lineage cells.
A form of pneumoconiosis caused by inhaled rare metal BERYLLIUM or its soluble salts which are used in a wide variety of industry including alloys, ceramics, radiographic equipment, and vacuum tubes. Berylliosis is characterized by an acute inflammatory reaction in the upper airway leading to BRONCHIOLITIS; PULMONARY EDEMA; and pneumonia.
Disease having a short and relatively severe course.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
A tetrameric enzyme that, along with the coenzyme NAD+, catalyzes the interconversion of LACTATE and PYRUVATE. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.
The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.
The study of the structure, growth, function, genetics, and reproduction of fungi, and MYCOSES.
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
Any disorder marked by obstruction of conducting airways of the lung. AIRWAY OBSTRUCTION may be acute, chronic, intermittent, or persistent.
A classification of lymphocytes based on structurally or functionally different populations of cells.
Long, pliable, cohesive natural or manufactured filaments of various lengths. They form the structure of some minerals. The medical significance lies in their potential ability to cause various types of PNEUMOCONIOSIS (e.g., ASBESTOSIS) after occupational or environmental exposure. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed, p708)
Toxins closely associated with the living cytoplasm or cell wall of certain microorganisms, which do not readily diffuse into the culture medium, but are released upon lysis of the cells.
Family of house dust mites, in the superfamily Analgoidea, order Astigmata. They include the genera Dermatophagoides and Euroglyphus.
An amine derived by enzymatic decarboxylation of HISTIDINE. It is a powerful stimulant of gastric secretion, a constrictor of bronchial smooth muscle, a vasodilator, and also a centrally acting neurotransmitter.
Interstitial pneumonia caused by extensive infection of the lungs (LUNG) and BRONCHI, particularly the lower lobes of the lungs, by MYCOPLASMA PNEUMONIAE in humans. In SHEEP, it is caused by MYCOPLASMA OVIPNEUMONIAE. In CATTLE, it may be caused by MYCOPLASMA DISPAR.
Measure of the maximum amount of air that can be expelled in a given number of seconds during a FORCED VITAL CAPACITY determination . It is usually given as FEV followed by a subscript indicating the number of seconds over which the measurement is made, although it is sometimes given as a percentage of forced vital capacity.
Soluble mediators of the immune response that are neither antibodies nor complement. They are produced largely, but not exclusively, by monocytes and macrophages.
Plasma glycoprotein member of the serpin superfamily which inhibits TRYPSIN; NEUTROPHIL ELASTASE; and other PROTEOLYTIC ENZYMES.
Asbestos. Fibrous incombustible mineral composed of magnesium and calcium silicates with or without other elements. It is relatively inert chemically and used in thermal insulation and fireproofing. Inhalation of dust causes asbestosis and later lung and gastrointestinal neoplasms.
A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.
A member of the MATRIX METALLOPROTEINASES that cleaves triple-helical COLLAGEN types I, II, and III.
Hypersensitivity reaction (ALLERGIC REACTION) to fungus ASPERGILLUS in an individual with long-standing BRONCHIAL ASTHMA. It is characterized by pulmonary infiltrates, EOSINOPHILIA, elevated serum IMMUNOGLOBULIN E, and skin reactivity to Aspergillus antigen.
Granulated cells that are found in almost all tissues, most abundantly in the skin and the gastrointestinal tract. Like the BASOPHILS, mast cells contain large amounts of HISTAMINE and HEPARIN. Unlike basophils, mast cells normally remain in the tissues and do not circulate in the blood. Mast cells, derived from the bone marrow stem cells, are regulated by the STEM CELL FACTOR.
Hypersensitivity reactions which occur within minutes of exposure to challenging antigen due to the release of histamine which follows the antigen-antibody reaction and causes smooth muscle contraction and increased vascular permeability.
Forceful administration into the peritoneal cavity of liquid medication, nutrient, or other fluid through a hollow needle piercing the abdominal wall.
A CXC chemokine with specificity for CXCR2 RECEPTORS. It has growth factor activities and is implicated as a oncogenic factor in several tumor types.
Relating to the size of solids.
The process in which the neutrophil is stimulated by diverse substances, resulting in degranulation and/or generation of reactive oxygen products, and culminating in the destruction of invading pathogens. The stimulatory substances, including opsonized particles, immune complexes, and chemotactic factors, bind to specific cell-surface receptors on the neutrophil.
The engulfing and degradation of microorganisms; other cells that are dead, dying, or pathogenic; and foreign particles by phagocytic cells (PHAGOCYTES).
A chronic multi-system disorder of CONNECTIVE TISSUE. It is characterized by SCLEROSIS in the SKIN, the LUNGS, the HEART, the GASTROINTESTINAL TRACT, the KIDNEYS, and the MUSCULOSKELETAL SYSTEM. Other important features include diseased small BLOOD VESSELS and AUTOANTIBODIES. The disorder is named for its most prominent feature (hard skin), and classified into subsets by the extent of skin thickening: LIMITED SCLERODERMA and DIFFUSE SCLERODERMA.
Group of chemokines with paired cysteines separated by a different amino acid. CXC chemokines are chemoattractants for neutrophils but not monocytes.
The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality.
A disease of chronic diffuse irreversible airflow obstruction. Subcategories of COPD include CHRONIC BRONCHITIS and PULMONARY EMPHYSEMA.
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
Short filamentous organism of the genus Mycoplasma, which binds firmly to the cells of the respiratory epithelium. It is one of the etiologic agents of non-viral primary atypical pneumonia in man.

GM-CSF-deficient mice are susceptible to pulmonary group B streptococcal infection. (1/5496)

Granulocyte-macrophage colony-stimulating factor (GM-CSF) gene-targeted mice (GM-/-) cleared group B streptococcus (GBS) from the lungs more slowly than wild-type mice. Expression of GM-CSF in the respiratory epithelium of GM-/- mice improved bacterial clearance to levels greater than that in wild-type GM+/+ mice. Acute aerosolization of GM-CSF to GM+/+ mice significantly enhanced clearance of GBS at 24 hours. GBS infection was associated with increased neutrophilic infiltration in lungs of GM-/- mice, while macrophage infiltrates predominated in wild-type mice, suggesting an abnormality in macrophage clearance of bacteria in the absence of GM-CSF. While phagocytosis of GBS was unaltered, production of superoxide radicals and hydrogen peroxide was markedly deficient in macrophages from GM-/- mice. Lipid peroxidation, assessed by measuring the isoprostane 8-iso-PGF2alpha, was decreased in the lungs of GM-/- mice. GM-CSF plays an important role in GBS clearance in vivo, mediated in part by its role in enhancing superoxide and hydrogen peroxide production and bacterial killing by alveolar macrophages.  (+info)

Molecular detection of tumor cells in bronchoalveolar lavage fluid from patients with early stage lung cancer. (2/5496)

BACKGROUND: Conventional cytologic analysis of sputum is an insensitive test for the diagnosis of non-small-cell lung cancer (NSCLC). We have recently demonstrated that polymerase chain reaction (PCR)-based molecular methods are more sensitive than cytologic analysis in diagnosing bladder cancer. In this study, we examined whether molecular assays could identify cancer cells in bronchoalveolar lavage (BAL) fluid. METHODS: Tumor-specific oncogene mutations, CpG-island methylation status, and microsatellite alterations in the DNA of cells in BAL fluid from 50 consecutive patients with resectable (stages I through IIIa) NSCLC were assessed by use of four PCR-based techniques. RESULTS: Of 50 tumors, 28 contained a p53 mutation, and the identical mutation was detected with a plaque hybridization assay in the BAL fluid of 39% (11 of 28) of the corresponding patients. Eight of 19 adenocarcinomas contained a K-ras mutation, and the identical mutation was detected with a mutation ligation assay in the BAL fluid of 50% (four of eight) of the corresponding patients. The p16 gene was methylated in 19 of 50 tumors, and methylated p16 alleles were detected in the BAL fluid of 63% (12 of 19) of the corresponding patients. Microsatellite instability in at least one marker was detected with a panel of 15 markers frequently altered in NSCLC in 23 of 50 tumors; the identical alteration was detected in the BAL fluid of 14% (three of 22) of the corresponding patients. When all four techniques were used, mutations or microsatellite instability was detected in the paired BAL fluid of 23 (53%) of the 43 patients with tumors carrying a genetic alteration. CONCLUSION: Although still limited by sensitivity, molecular diagnostic strategies can detect the presence of neoplastic cells in the proximal airway of patients with surgically resectable NSCLC.  (+info)

Localization of a candidate surfactant convertase to type II cells, macrophages, and surfactant subfractions. (3/5496)

Pulmonary surfactant exists in the alveolus in several distinct subtypes that differ in their morphology, composition, and surface activity. Experiments by others have implicated a serine hydrolase in the production of the inactive small vesicular subtype of surfactant (N. J. Gross and R. M. Schultz. Biochim. Biophys. Acta 1044: 222-230, 1990). Our laboratory recently identified this enzyme in the rat as the serine carboxylesterase ES-2 [F. Barr, H. Clark, and S. Hawgood. Am. J. Physiol. 274 (Lung Cell. Mol. Physiol. 18): L404-L410, 1998]. In the present study, we determined the cellular sites of expression of ES-2 in rat lung using a digoxygenin-labeled ES-2 riboprobe. ES-2 mRNA was localized to type II cells and alveolar macrophages but not to Clara cells. Using a specific ES-2 antibody, we determined the protein distribution of ES-2 in the lung by immunohistochemistry, and it was found to be consistent with the sites of mRNA expression. Most of the ES-2 in rat bronchoalveolar lavage is in the surfactant-depleted supernatant, but ES-2 was also consistently localized to the small vesicular surfactant subfraction presumed to form as a consequence of conversion activity. These results are consistent with a role for endogenous lung ES-2 in surfactant metabolism.  (+info)

Expression of heat shock protein 72 by alveolar macrophages in hypersensitivity pneumonitis. (4/5496)

The current study was done to look at a possible role of heat shock proteins (HSPs) in hypersensitivity pneumonitis (HP). The specific aims were to determine whether there was a difference in the expression of HSP72 in alveolar macrophages (AMs) between mice challenged with HP antigen and saline-treated control mice and between AMs obtained by bronchoalveolar lavage from 18 patients with HP and 11 normal subjects. The expression of HSP72 was studied under basal conditions and under a mild heat shock. HSP72 expression by AMs in response to in vitro stimulation with Saccharopolyspora rectivirgula was lower in AMs of control mice than in those of HP animals. HSP72 was constitutively expressed in AMs of both normal and HP subjects. Densitometric ratios showed that AMs from normal subjects responded to heat shock with a 39 degrees C-to-37 degrees C ratio of 1.72 +/- 0.18 (mean +/- SE), and AMs from HP patients responded with a ratio of 1.16 +/- 0.16 (P = 0.0377). This decreased induction by additional stress of AMs could lead to an altered immunoregulatory activity and account for the inflammation seen in HP.  (+info)

A rapid polymerase chain reaction technique for detecting M tuberculosis in a variety of clinical specimens. (5/5496)

A rapid in-house polymerase chain reaction (PCR) assay is described for the direct detection of Mycobacterium tuberculosis complex in clinical material. Its performance is compared with two kit based systems. The results of the in-house assay were comparable with the commercial assays, detecting M tuberculosis in 100% of smear positive, culture positive samples. The in-house assay proved to be rapid, easy, and inexpensive to perform, and the inclusion of an internal inhibitor control permitted validation of the PCR results.  (+info)

Cigarette smoking decreases interleukin-8 secretion by human alveolar macrophages. (6/5496)

Cigarette smoking can impair pulmonary immune function, and hence influences the development of lung diseases. Interleukin-8 (IL-8) is a proinflammatory peptide and a potent chemotactic factor for neutrophils, and is produced by both immune and non-immune cells including monocytes and alveolar macrophages (AM). We investigated the effect of cigarette smoking on the secretion of IL-8 by human AM. The IL-8 concentration in bronchoalveolar lavage fluid (BALF) was much higher in smokers than in non-smokers (18.4 +/- 3.9 vs 4.1 +/- 1.0 pg ml-1; P < 0.005). However, spontaneous IL-8 secretion by cultured AM was lower in smokers than in non-smokers (46.8 +/- 12.7 vs 124.1 +/- 24.0 ng ml-1; P < 0.01). When stimulated with lipopolysaccharide (LPS), AM from smokers secreted significantly less IL-8 than those from non-smokers at all tested concentrations of LPS. In contrast, the amount of IL-8 secreted by peripheral blood monocytes with or without LPS stimulation was comparable in smokers and non-smokers. These observations indicate that smoking decreases IL-8 secretion by AM, which may modify or decrease the inflammatory response in the lung.  (+info)

Effect of hyperoxia on human macrophage cytokine response. (7/5496)

In the development of lung damage induced by oxidative stress, it has been proposed that changes in alveolar macrophages (AM) function with modifications in cytokine production may contribute to altered repair processes. To characterize the changes in profiles of cytokine production by macrophages exposed to oxidants, the effects of hyperoxia (95% O2) on interleukin (IL)-1 beta, IL-6, IL-8, and tumour necrosis factor-alpha (TNF-alpha) expression were studied. Experiments were first performed using AM obtained from control subjects and children with interstitial lung disease. Results showed that a 48 h O2 exposure was associated with two distinct patterns of response: a decrease in TNF-alpha, IL-1 beta and IL-6 expression, and an increase in IL-8. To complete these observations we used U937 cells that were exposed for various durations to hyperoxia. We confirmed that a 48 h O2 exposure led to similar changes with a decrease in TNF-alpha, IL-1 beta and IL-6 production and an increase in IL-8. Interestingly, this cytokine response was preceded during the first hours of O2 treatment by induction of TNF-alpha, IL-1 beta and IL-6. These data indicate that hyperoxia induces changes in the expression of macrophages inflammatory cytokines, and that these modifications appear to be influenced by the duration of O2 exposure.  (+info)

Pneumonia in febrile neutropenic patients and in bone marrow and blood stem-cell transplant recipients: use of high-resolution computed tomography. (8/5496)

PURPOSE: To obtain statistical data on the use of high-resolution computed tomography (HRCT) for early detection of pneumonia in febrile neutropenic patients with unknown focus of infection. MATERIALS AND METHODS: One hundred eighty-eight HRCT studies were performed prospectively in 112 neutropenic patients with fever of unknown origin persisting for more than 48 hours despite empiric antibiotic treatment. Fifty-four of these studies were performed in transplant recipients. All patients had normal chest roentgenograms. If pneumonia was detected by HRCT, guided bronchoalveolar lavage was recommended. Evidence of pneumonia on chest roentgenograms during follow-up and micro-organisms detected during follow-up were regarded as documentation of pneumonia. RESULTS: Of the 188 HRCT studies, 112 (60%) showed pneumonia and 76 were normal. Documentation of pneumonia was possible in 61 cases by chest roentgenography or micro-organism detection (54%) (P < 10(-6)). Sensitivity of HRCT was 87% (88% in transplant recipients), specificity was 57% (67%), and the negative predictive value was 88% (97%). A time gain of 5 days was achieved by the additional use of HRCT compared to an exclusive use of chest roentgenography. CONCLUSION: The high frequency of inflammatory pulmonary disease after a suspicious HRCT scan (> 50%) proves that pneumonia is not excluded by a normal chest roentgenogram. Given the significantly longer duration of febrile episodes in transplant recipients, HRCT findings are particularly relevant in this subgroup. Patients with normal HRCT scans, particularly transplant recipients, have a low risk of pneumonia during follow-up. All neutropenic patients with fever of unknown origin and normal chest roentgenograms should undergo HRCT.  (+info)

TY - JOUR. T1 - Use of Aspergillus fumigatus real-time PCR in bronchoalveolar lavage samples (BAL) for diagnosis of invasive aspergillosis, including azole-resistant cases, in high risk haematology patients: the need for a combined use with galactomannan. AU - Sanguinetti, Maurizio. AU - De Carolis, Elena. AU - Mikulska, Malgorzata. AU - Furfaro, Elisa. AU - Drago, Enrico. AU - Pulzato, Ilaria. AU - Borghesi, Maria Lucia. AU - Zappulo, Emanuela. AU - Raiola, Anna Maria. AU - Grazia, Carmen Di. AU - Bono, Valerio Del. AU - Cittadini, Giuseppe. AU - Angelucci, Emanuele. AU - Viscoli, Claudio. PY - 2019. Y1 - 2019. N2 - Diagnosis of invasive aspergillosis (IA) is challenging, particularly in high-risk patients with lung lesions other than typical according to 2008-EORTC/MSG criteria. Even if microbiology is positive, they still remain unclassified according to 2008-EORTC/MSG. Quantitative polymerase chain reaction (qPCR) provides new mycological documentation of IA. This retrospective study ...
The objective of this study was to review the literature on bronchoalveolar lavage fluid cell profiles in healthy children and adolescents, as well as on the use of BAL as a diagnostic and follow-up tool for lung disease patients in this age bracket. To that end, we used the Medline database, compiling studies published between 1989 and 2009 employing the following MeSH descriptors (with Boolean operators) as search terms: bronchoalveolar lavage AND cytology OR cell AND child. In healthy children, the cell profile includes alveolar macrophages (> 80%), lymphocytes (approximately 10%), neutrophils (approximately 2%) and eosinophils (< 1%). The profile varies depending on the disease under study. The number of neutrophils is greater in wheezing children, especially in non-atopic children, as well as in those with pulmonary infectious and inflammatory profiles, including cystic fibrosis and interstitial lung disease. Eosinophil counts are elevated in children/adolescents with asthma and can reach ...
TY - JOUR. T1 - Increased angiostatin levels in bronchoalveolar lavage fluids from ARDS patients and from human volunteers after lung instillation of endotoxin. AU - Lucas, Rudolf. AU - Lijnen, H. Roger. AU - Suffredini, Anthony F.. AU - Pepper, Michael S.. AU - Steinberg, Kenneth P.. AU - Martin, Thomas R.. AU - Pugin, Jérôme. PY - 2002/1/1. Y1 - 2002/1/1. N2 - Acute respiratory distress syndrome (ARDS) is characterized by a disruption of the alveolar-capillary barrier, due to both an epithelial and an endothelial dysfunction. Whereas epithelial apoptosis seems to be mainly mediated by Fas ligand, the mediators of endothelial damage remain to be identified. Angiostatin, a powerful inhibitor of angiogenesis in vivo, also specifically induces apoptosis in endothelial cells. The concentration of various enzymes that cleave angiostatin from plasminogen was reported to be significantly increased in bronchalveolar lavage (BAL) fluids from patients with ARDS. Therefore, in this study, we ...
PAN Czytelnia Czasopism, Cytological evaluation of tracheal aspirate and broncho-alveolar lavage fluid in comparison to endoscopic assessment of lower airways in horses with recurrent airways obstruction or inflammatory airway disease - Polish Journal of Veterinary Sciences
The usefulness of bronchoalveolar lavage in the diagnosis of pulmonary infiltrates in the immunosuppressed patient was studied in 97 patients. In immunosuppressed patients, the available diagnostic procedures are often invasive and have variable yield and a potential for serious complications. Bronchoalveolar lavage had an overall diagnostic yield of 66% (61 of 92 diseases). It was most effective in the diagnosis of opportunistic infections, including infection with Pneumocystis carinii (18 of 22 cases), cytomegalovirus pneumonia (10 of 12 cases), fungal pneumonia (5 of 6 cases), and mycobacterial disease (4 of 5 cases). The technique was also helpful in confirming pulmonary hemorrhage (7 of 9 cases) but was less useful for diagnosing malignancy (10 of 22 cases) and drug-induced toxicity (6 of 15 cases). Findings of bronchoalveolar lavage were additive with those of transbronchial biopsies, brushings, and washings in the diagnosis of most of the diseases. The procedure was safe, even in ...
en] An increased production of NO* and peroxynitrite in lungs has been suspected during acute lung injury (ALI) in humans, and recent studies provided evidence for an alveolar production of nitrated compounds. We observed increased concentrations of nitrites/nitrates, nitrated proteins and markers of neutrophil degranulation (myeloperoxidase, elastase and lactoferrine) in the fluids recovered from bronchoalveolar lavage fluids (BALF) of patients with ALI and correlated these changes to the number of neutrophils and the severity of the ALI. We also observed that BALFs stimulated the DNA-binding activity of the nuclear transcription factor kappa B (NF-kappaB) as detected by electrophoretic mobility shift assay in human alveolar cells (A549) and monocytes (THP1). The level of activation of the NF-kappaB-binding activity was correlated to the concentration of nitrated proteins and myeloperoxidase. Furthermore, in vitro studies confirmed that NO*-derived species (peroxynitrite and nitrites) and the ...
Several components of cellular and humoral immunity were examined in bronchoalveolar lavage fluid and blood of 15 patients with the acquired immunodeficiency syndrome, and the results were compared to data from 25 healthy controls (including 5 asymptomatic homosexual men). Compared with that of controls, bronchoalveolar lavage fluid from patients tended to have more lymphocytes and significantly more neutrophils; a lower OKT4/OKT8 ratio, due to an increase in total OKT8 cells; and normal total OKT4 cell counts, despite a significant decrease in numbers of OKT4 cells in peripheral blood. Patients also had significantly more IgG-releasing cells and higher IgG levels than controls in lavage fluid. These data show that, in the lung lining fluid of patients with the acquired immunodeficiency syndrome, significant alterations in cellular and humoral immunity exist that differ in several important respects from immunity in controls and from corresponding changes in patients peripheral blood. ...
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Proteome analysis of bronchoalveolar lavage fluids reveals host and fungal proteins highly expressed during invasive pulmonary aspergillosis in mice and humans:
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TY - JOUR. T1 - Novel approach for analysis of bronchoalveolar lavage fluid (BALF) using HPLC-QTOF-MS-based lipidomics. T2 - Lipid levels in asthmatics and corticosteroid-treated asthmatic patients. AU - Kang, Yun Pyo. AU - Lee, Won Jun. AU - Hong, Ji Yeon. AU - Lee, Sae Bom. AU - Park, Jeong Hill. AU - Kim, Donghak. AU - Park, Sunghyouk. AU - Park, Choon Sik. AU - Park, Sung Woo. AU - Kwon, Sung Won. PY - 2014/9/5. Y1 - 2014/9/5. N2 - To better understand the respiratory lipid phenotypes of asthma, we developed a novel method for lipid profiling of bronchoalveolar lavage fluid (BALF) using HPLC-QTOF-MS with an internal spectral library and high-throughput lipid-identifying software. The method was applied to BALF from 38 asthmatic patients (18 patients with nonsteroid treated bronchial asthma [NSBA] and 20 patients with steroid treated bronchial asthma [SBA]) and 13 healthy subjects (NC). We identified 69 lipids, which were categorized into one of six lipid classes: lysophosphatidylcholine ...
Patrolling Monocytes Promote the Pathogenesis of Early Lupus-Like Glomerulonephritis Researchers found that glomerulonephritis was driven by TLRs but, remarkably, proceeded independently of immune complexes. Rather, disease in three different mouse models and patients with systemic lupus erythematosus was characterized by glomerular accumulation of patrolling monocytes, a cell type with an emerging key function in vascular inflammation. [J Clin Invest] Full Article Lipopolysaccharide Inhalation Recruits Monocytes and Dendritic Cell Subsets to the Alveolar Airspace The authors used lipopolysaccharide inhalation to induce acute inflammation in healthy volunteers and examined the impact on bronchoalveolar lavage fluid and blood mononuclear phagocyte repertoire. Classical monocytes and two dendritic cell subsets were expanded in bronchoalveolar lavage fluid eight hours after lipopolysaccharide inhalation. [Nat Commun] Full Article FcγRIIb Differentially Regulates Pre-Immune and Germinal Center B ...
TY - JOUR. T1 - Increased levels of glutathione in bronchoalveolar lavage fluid from patients with asthma. AU - Smith, L. J.. AU - Houston, M.. AU - Anderson, J.. PY - 1993. Y1 - 1993. N2 - Patients with asthma generate increased amounts of reactive oxygen species (ROS) from peripheral blood cells and cells recovered by bronchoalveolar lavage (BAL). ROS produce many of the pathophysiologic changes associated with asthma and may contribute to its pathogenesis. Although antioxidant defenses inhibit the changes produced by ROS, no data are available on local antioxidant defenses in asthma. The present study was designed to begin to explore these defenses by measuring superoxide dismutase (SOD) and catalase activities and total glutathione (GSH) levels in BAL fluid from normal subjects and patients with mild asthma. Baseline pulmonary function and methacholine bronchoprovocation tests were performed on all subjects. BAL was achieved by instilling five 20-ml aliquots of phosphate-buffered saline in ...
Background: Cytologic examination of specimens obtained from the respiratory tract is a lung cancer diagnostic procedure with high specificity, but moderate sensitivity. The use of molecular biomarkers may enhance the sensitivity of cytologic examination in the detection of lung cancer. Methods: Complement factor H, a protein secreted by lung cancer cells, was quantified in a series of bronchoalveolar lavage supernatants from lung cancer patients and patients with non-malignant respiratory diseases. Albumin, total protein content, and hemoglobin were also analyzed. Results were validated in independent sets of bronchoalveolar lavage and sputum supernatants. Results: There was a significantly higher concentration of factor H in bronchoalveolar lavage samples from lung cancer patients. The sensitivity and specificity of the factor H test was 82% and 77%, respectively. These results were validated in an independent set of patients with nearly identical results. Furthermore, 70% and 45% of ...
The purpose of this study is to explore the correlations of interleukin 36 (IL-36) and Soluble B7-H3 (sB7-H3) levels in bronchoalveolar lavage fluid (BALF) with clinical characteristics and laboratory findings. A total of 35 children with M. pneumnoiae pneumonia (MPP) and 15 control subjects were enrolled. BALF concentrations of sB7-H3 and IL-36 were detected using enzyme-linked immunosorbent assays and clinical profiles of children with MPP were obtained. Children with MPP had significantly higher levels of sB7-H3 and IL-36 compared to control subjects (both P < 0.05). Meanwhile, children with pleural effusion had significantly higher levels of sB7-H3 and IL-36 compared to children without pleural effusion (both P < 0.05). BALF concentration of sB7-H3 was strongly associated with concentration of IL-36 (r = 0.796, P < 0.0001) and sB7-H3 was correlated with duration of fever (r = 0.427, P = 0.11) and length of stay (r = 0.345, P = 0.043). Both concentrations of sB7-H3 and IL-36 were significantly
Vitamin E acetate was universally found in bronchoalveolar lavage fluid samples from patients with vaping lung injury, providing the first direct evidence of vitamin E acetate at the site of injury.
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Desk E1 in the online supplement). using ELISA (Invitrogen). Bronchoalveolar lavage fluid (BALF) was analyzed for total, differential cell counts, and IL-18 ELISA. Left lung cells was examined by eosin and hematoxylin, immunohistochemical, and immunofluorescence staining, and homogenates had been ready for IL-1, IL-18 (Invitrogen), and IL-33 (R&D Systems) quantitative ELISA. Best lungs were utilized to measure wet-to-dry lung pounds ratio (online health supplement). Mouse Microarray Evaluation Total RNA was extracted from lung cells of ventilated and control NOD/shi mice. Microarray manifestation profiles were produced using Ref-8 mouse arrays (Illumina) based on the producers process. The IGF2R microarray data can be found through the GEO accession quantity type:entrez-geo,attrs:text:GSE29920″,term_id:29920″GSE29920. Gene manifestation was verified using quantitative TaqMan REAL-TIME PCR (online health supplement). Mouse IL-18CNeutralizing Antibody Treatment C57Bl/6 mice (= ...
bronchoalveolar lavage,mechanical ventilation,platelet activating factor,surfactant,ventilator-associated lung injury,respiratory-distress syndrome,surfactant aggregate conversion,mechanical ventilation,pulmonary-surfactant,alveolar surfactant,dogs lungs,in-vitro,cytokines,clearance, ...
Cytokine Levels In Bronchoalveolar Lavage Fluid (Balf) In Patients With Systemic Sclerosis (SSc). In comparison to other diseases, SSc patients present a spe...
C Ward, Kelly CA, Stenton SC, M Duddridge, Hendrick DJ, Walters EH; Macrophage (AM) and Neutrophil (PMNL) Chemiliuminescence (CL) in Bronchoalveolar Lavage (BAL) Fluid. Clin Sci (Lond) 1 December 1987; 73 (s17): 33P-34P. doi: https://doi.org/10.1042/cs073033Pc. Download citation file:. ...
Bronchoscopy with bronchoalveolar lavage. Refractory pneumonitis. A 69-year-old man status post trauma, slightly prolonged respiratory failure status post tracheostomy, requires another bronchoscopy for further evaluation of refractory pneumonitis.
TY - JOUR. T1 - Endothelin-1 in bronchoalveolar lavage during rejection of allotransplanted lungs. AU - Schersten, Henrik. AU - Aarnio, Pertti. AU - Burnett, John C.. AU - McGregor, Christopher G.A.. AU - Miller, Virginia M.. PY - 1994/1. Y1 - 1994/1. UR - http://www.scopus.com/inward/record.url?scp=0028107330&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0028107330&partnerID=8YFLogxK. U2 - 10.1097/00007890-199401000-00027. DO - 10.1097/00007890-199401000-00027. M3 - Article. C2 - 8291104. AN - SCOPUS:0028107330. VL - 57. SP - 159. EP - 161. JO - Transplantation. JF - Transplantation. SN - 0041-1337. IS - 1. ER - ...
TY - JOUR. T1 - Bronchoalveolar lavage in the study of asthma. AU - Rennard, S. I.. AU - Thompson, A. B.. AU - Floreani, A. A.. PY - 1992. Y1 - 1992. UR - http://www.scopus.com/inward/record.url?scp=0026770648&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0026770648&partnerID=8YFLogxK. U2 - 10.1378/chest.102.1.1. DO - 10.1378/chest.102.1.1. M3 - Editorial. C2 - 1623734. AN - SCOPUS:0026770648. VL - 102. SP - 1. EP - 2. JO - Chest. JF - Chest. SN - 0012-3692. IS - 1. ER - ...
Cytology, Bronchoalveolar Lavage 2,ARUP Laboratories is a national reference laboratory and a worldwide leader in innovative laboratory research and development. ARUP offers an extensive test menu of highly complex and unique medical tests in clinical and anatomic pathology. Owned by the University of Utah, ARUP Laboratories client,medicine,medical supply,medical supplies,medical product
In this study we have analysed the phenotype of CD4+ and CD8+ T lymphocytes from PBL and BAL fluid, as well as peripheral blood monocytes and alveolar macrophages from patients with sarcoidosis. Cells were studied with respect to expression of various surface activation markers, co-stimulatory and adhesion molecules. In addition, we investigated differences between cells from patients with active and inactive disease.. Our results highlight the role of T cells in sarcoidosis as there was an increased expression by BAL lymphocytes of several of the molecules studied-for example, CD26, CD95 (Fas), and the activation markers CD69 and gp240. This is in agreement with previous studies investigating other activation markers such as IL-2R (CD25), very late activation antigen-1 (VLA-1), CD49a, CD49d, and HLA-DR.11-14 A role for the receptor-ligand adhesion molecule pair CD11a/CD18-CD54 is indicated by our demonstration of increased expression of CD11a on alveolar macrophages as well as on peripheral ...
bronchoalveolar definition: Adjective (comparative more bronchoalveolar, superlative most bronchoalveolar) 1. Relating to both the bronchi and the alvioli of the lungs...
The pathophysiological role of inflammation in lung diseases including asthma and chronic obstructive pulmonary disease is well established. Quantifying lung inflammation is relevant for the management of inflammatory airway diseases as it may indicate that pharmacological intervention is required before symptom onset and reduction in lung function. Moreover, monitoring of airway inflammation might be useful in the follow-up of patients with asthma and COPD, and for guiding pharmacological therapy. Quantification of pulmonary inflammation is currently based more on invasive methods including the analysis of broncho-alveolar lavage fluid, bronchoscopy, and bronchial biopsies, semi-invasive methods such as sputum induction, and the measurement of inflammatory biomarkers in plasma and urine, which are likely to reflect systemic rather than lung inflammation. Interest in the identification of non-invasive biomarkers for inflammatory airway diseases has been growing. Along these years less invasive ...
Pulmonary infection is normally a major reason behind mortality and morbidity as well as the magnitude from the lung inflammatory response correlates with affected person survival. and peptidoglycan (PGN) implemented intratracheally significantly elevated the amounts of neutrophils retrieved in the bronchoalveolar lavage liquid (BALF). F344 got approximately 10-flip even more neutrophils in the BALF weighed against DA (< 0.001) and higher BALF concentrations of total proteins tumor necrosis aspect-α and macrophage inflammatory proteins 2. LTA/PGN administration in DA×F344 congenic strains (with LTA/PGN created significantly higher degrees of keratinocyte-derived chemokine and macrophage inflammatory proteins 2 than alveolar macrophages from DA rats. The distinctions had been linked to differential mitogen-activated proteins kinase phosphorylation. We conclude the fact that factors adding to irritation could be site and problem dependent. A better knowledge of site-specific irritation can lead ...
Airborne Fe2O3 nanoparticles (NPs) with the mean diameter of 14 4 nm produced at spark ablation from 99.99% pure iron rods were fed into a nose-only exposure tower for rats exposed for 4 h a day, 5 days a week during 3, 6 or 10 months at a mean concentration of 1.14 0.01 mg/m3. Nanoparticles filtered out of the air exhausted from the exposure tower proved insoluble in de-ionized water but gradually dissolved in the cell free fluid supernatant produced by broncho-alveolar lavage and in the sterile bovine blood serum. The Fe2O3 content in lungs was measured by the Electron Paramagnetic Resonance (EPR) spectroscopy. NP retention in lungs and in brain was visualized with the Transmission Electron Microscopy (TEM). It was found a relatively low but significant pulmonary accumulation of Fe2O3, gradually increasing with time but tending to attain an equilibrium level. Besides, TEM-images showed nanoparticles retention within alveolocytes and the myelin sheaths of brain fibers associated with their ...
Endoscopy may be recommended- this is when we place a camera down into the airway and we can visualise for any signs of infection. We are also then able to take samples (tracheal wash and broncho-alveolar lavage) from the airway to confirm if your horse is suffering from asthma and whether any secondary infections are present ...
Jan Springer, P. Lewis White, Johanna Kessel, Imke Wieters, Daniel Teschner, Daniel Korczynski, Tobias Liebregts, Oliver A. Cornely, Stefan Schwartz, Thomas Elgeti, Lisa Meintker, Stefan W. Krause, Raquel B. Posso, Werner J. Heinz, Sandra Fuhrmann, Jörg Janne Vehreschild, Hermann Einsele, Volker Rickerts, Juergen Loeffler ...
The paper is concerned with the description of clinical, x-ray and morphological investigation of 123 bronchoalveolar cancer patients. Three types of this disease were defined: nodular (homogeneous and nonhomogeneous), pneumonia-like (infiltrative and infiltrative-nodular) and mixed (focal-disseminated, focal-nodular and focal-infiltrative). These types of bronchoalveolar cancer are most probably stages of the same tumor process. Clinical and x-ray signs of each type showed correlation with a morphological picture of a tumor. Shadow nonhomogeneity as one of the main x-ray signs of bronchoalveolar cancer was shown to be determined by the alveolar structure of a tumor, a tendency to the formation of small cavities, filled with viscous mucosa and air. Correct clinical and x-ray diagnosis in all types of bronchoalveolar cancer (before the use of the morphological methods) was established in 45.5% of the patients ...
The paper is concerned with the description of clinical, x-ray and morphological investigation of 123 bronchoalveolar cancer patients. Three types of this disease were defined: nodular (homogeneous and nonhomogeneous), pneumonia-like (infiltrative and infiltrative-nodular) and mixed (focal-disseminated, focal-nodular and focal-infiltrative). These types of bronchoalveolar cancer are most probably stages of the same tumor process. Clinical and x-ray signs of each type showed correlation with a morphological picture of a tumor. Shadow nonhomogeneity as one of the main x-ray signs of bronchoalveolar cancer was shown to be determined by the alveolar structure of a tumor, a tendency to the formation of small cavities, filled with viscous mucosa and air. Correct clinical and x-ray diagnosis in all types of bronchoalveolar cancer (before the use of the morphological methods) was established in 45.5% of the patients ...
To transport BAL samples from the field, pour samples into EDTA tubes or dilute 50:50 with grain alcohol or Vodka (100 Proof) and transport in clot tubes. Dont drink and drive! ...
This project will identify the characteristic immune cell profiles for two patient groups with allergic reactions with or without specific IgE in serum, also compared with healthy controls.
The figure above shows the overall detectability of the specified assay in a range of different mouse sample types. Detectability in each sample matrix is presented as the mean % of all samples included in the validation studies that could be measured above the limit of detection (LOD).. Dilution of samples may be necessary for some sample matrices other than plasma or serum to achieve optimal assay performance - the data shown above represents the values obtained at the optimal dilution identified in each case. Details regarding possible dilutions should be discussed with your Olink representative prior to commencing the study. The data is compiled from multiple studies comprising a range of healthy and disease samples, including samples from genetically modified mouse lines in some cases.. (Note: CSF = cerebrospinal fluid; BAL = bronchoalveolar lavage).. ...
Source: Adapted from the National Institutes of Health. What does the term lavage mean? The term lavage refers to washing out of an organ, especially the stomach. To find out more about this term, please search the news section of this website for related articles and information.. ...
Trimesulfin Balsámico is a medicine available in a number of countries worldwide. A list of US medications equivalent to Trimesulfin Balsámico is available on the Drugs.com website.
Confused on this one. Inpatient visist: When a Intensivist does a procedure of BAL Lavage and its done on both lungs. Is this report twice with CPT 3
differential cell counting in mixed cultures - posted in Tissue and Cell Culture: hello, i will be culturing neurons and macrophages together in a mixed culture. does anyone know of any methods for counting one cell type alone?
Also known as: bal, Bal., Bal, BaL, Ba.L, Ba-L. An, Ba-L, BA¶l, BAl, BAL., BAL, BA-L, B=Al, B.A.L., B.A.L, B. A. L., B-aL, B-AL ...
Le laveur de microplaques 405LS est la cinquième génération de laveurs BioTek, reconnus pour leur niveau de performance et durabilité. Sur ces nouveaux modèles, les vannes de sélection de solution de lavage sont intégrées et un nouveau design de peigne de lavage facilite le remplacement du peigne.
It was initially thought the use of chemical-grade silicone was limited to implants made during the past decade, but the TGA today said there were indications from European regulators the problem extended back to the late 1990s.
Data & statistics on Acute Lung Injury: MRNA expression of Interleukin-8 in bronchoalveolar lavage fluid cells before injury and after injury. NG, acute lung injury with normoglycemia group; HG, acute lung injury with hyperglycemia group; HG-VI, acute lung injury with hyperglycemia treated with intravenous insulin group; HG-AI, acute lung injury with hyperglycemia treated with aerosolized insulin group; IL-8, interleukin-8. Boxes extend ..., Particle-induced acute lung injury. Results obtained from analysis performed on bronchoalveolar lavage fluid samples recovered from animals at 24 h post-exposure. Animals were exposed by intratracheal instillation to: saline ( E); OFA100 ( C); OFA400 (P); ROFA#6 ( 1); or ARD ([). Various biomarkers of acute lung injury were assessed such as: A) edema/secretory activity (protein/mL); B) edema (albumin/mL); ..., Inhaltsverzeichnis Einleitung Hypothesen Theoretische Grundlagen der Hypothesen Patienten und Methodik Patienten Pneumonie Acute lung injury / Acute
TY - JOUR. T1 - Removal of bronchoalveolar cells augments the late eosinophilic response to segmental allergen challenge. AU - Hunt, L. W.. AU - Gleich, G. J.. AU - Kita, H.. AU - Weiler, D. A.. AU - Schroeder, D. R.. AU - Vuk Pavlovic, Z.. AU - Sur, Sanjiv. PY - 2002. Y1 - 2002. N2 - Background: In patients with quiescent asthma, macrophages are the most prevalent cells recovered by bronchoalveolar lavage (BAL). Through activation via their FcεRII receptors or by acting as antigen-presenting cells, macrophages could, in theory, promote the late airway response to allergen. Objective: In order to investigate the importance of macrophages and other airway luminal cells in inducing the late airway response, a novel washout experiment was designed. Methods: Five patients with ragweed-allergic asthma underwent bronchoscopy and segmental bronchial challenge with either normal saline or short ragweed extract in two segments of one lung. In a third segment of the opposite lung, 12 successive BALs ...
Invasive pulmonary aspergillosis (IPA) remains a major cause for morbidity and mortality in patients (pts) with hematologic malignancies. As culture-based methods only yield results in a minority of patients, using non-culture-based methods for detection of aspergillosis in clinical specimens becomes increasingly important. Analyzing bronchoalveolar lavage (BAL) samples with polymerase chain reaction (PCR) is promising, however, the influence of current antifungal drugs on the performance of this diagnostic tool remains controversial.. The aim of the trial is to elucidate on the performance of BAL PCR under antifungal treatment.. Patients with high risk of invasive aspergillosis and lung infiltrates are sampled via BAL, the sample is analyzed for fungal DNA by Apsergillus specific PCR. Clinical data including treatment data is assessed and evaluated. ...
TY - JOUR. T1 - Noninvasive quantification of airway inflammation following segmental allergen challenge with functional MR imaging. T2 - A proof of concept study. AU - Renne, Julius. AU - Hinrichs, Jan. AU - Schönfeld, Christian. AU - Gutberlet, Marcel. AU - Winkler, Carla. AU - Faulenbach, Cornelia. AU - Jakob, Peter. AU - Schaumann, Frank. AU - Krug, Norbert. AU - Wacker, Frank. AU - Hohlfeld, Jens M.. AU - Vogel-Claussen, Jens. PY - 2015/1/1. Y1 - 2015/1/1. N2 - Purpose: To evaluate oxygen-enhanced T1-mapping magnetic resonance (MR) imaging as a noninvasive method for visualization and quantification of regional inflammation after segmental allergen challenge in asthmatic patients compared with control subjects.Materials and After institutional review board approval, nine asthmatic Methods: and four healthy individuals gave written informed consent. MR imaging (1.5 T) was performed by using an inversion-recovery snapshot fast low-angle shot sequence before (0 hours) and 6 hours and 24 hours ...
CoV 2019, whose genetic material is single-strand RNA and a member of the corona family, was identified in November 2019, it is a pathogen with a strong pandemic effect. CoV 2019 (Genbank acc. MN908947), whose genome has been sequenced, has high similarity to the SARS virus. Test-it COVID-19 Real Time RT-PCR Diagnostic Kit detects SARS-CoV-2. (2019-nCoV) from respiratory tract samples (nasopharyngeal, oropharyngeal, sputum and bronchoalveolar lavage samples). COVID-19 Real Time RT-PCR Diagnostic Kit scans the N1, N2 gene regions on the N gene determined by WHO and USA-CDC.. COVID-19 Real Time RT-PCR Diagnostic Kit detects SARS-CoV-2. (2019nCoV) RNA from respiratory tract samples (nasopharyngeal, oropharyngeal, sputum and bronchoalveolar lavage samples). In the diagnostic kit, RNAse P (RP) mRNA at the human mRNA level was chosen as the internal control. The kit, which receives radiation from FAM and HEX wavelengths, works in multiplex with a single tube.. ...
Myeloid dendritic cells (DCs) are increased in the airway wall of patients with chronic obstructive pulmonary disease (COPD), and postulated to play a crucial role in COPD. However, DC phenotypes in COPD are poorly understood. Function-associated surface molecules on bronchoalveolar lavage fluid (BALF) DCs were analyzed using flow cytometry in current smokers with COPD, in former smokers with COPD and in never-smoking controls. Myeloid DCs of current smokers with COPD displayed a significantly increased expression of receptors for antigen recognition such as BDCA-1 or Langerin, as compared with never-smoking controls. In contrast, former smokers with COPD displayed a significantly decreased expression of these receptors, as compared with never-smoking controls. A significantly reduced expression of the maturation marker CD83 on myeloid DCs was found in current smokers with COPD, but not in former smokers with COPD. The chemokine receptor CCR5 on myeloid DCs, which is also important for the uptake and
Background. In acute respiratory distress syndrome or pneumonia, a procoagulant shift is observed in bronchoalveolar lavage fluid (BALF). The effect of a primarily extrapulmonary infection on coagulation and fibrinolysis in the pulmonary compartment is unclear. Methods. In 35 patients, 87 bronchoalveolar lavages were performed on the day of operation for secondary peritonitis (day 0) and on days 2 and 3 after surgery. Two noninfectious control groups were included: subjects undergoing bronchoalveolar lavage after elective surgery (n = 8) and those undergoing long-term mechanical ventilation (n = 10). Results. In BALF from patients with peritonitis, a tissue factor (TF)/factor VIIaâ€mediated activation of coagulation was shown (high levels of thrombin-antithrombin complexes). Levels of fibrinolysis activators decreased rapidly after day 0, whereas levels of inhibitors increased. The net effect was reduced fibrinolysis (plasminogen activator activity). The sequential comparison of plasma ...
BackgroundPneumonia carries significant morbidity and mortality in leukemic and bone marrow transplant patient. The development of pulmonary infiltrates in the setting of such immunocompromise raises concern for both infectious and non-infectious etiologies, some of which are potentially treatabl. Performing bronchoscopy provides several different options for sampling the lower respiratory tract. Among these, bronchoalveolar lavage (BAL) is especially effective at collecting samples from the alveoli and has been shown to be associated with less risk than transbonchial biops. We sought to examine the effect of antimicrobial treatment on BAL results in a large study population of leukemic and bone marrow transplant patients.Subjects and MethodsThis retrospective chart review was performed at a single academic cancer center. A power analysis was performed to determine the appropriate sample size. The patients were selected from those who had undergone an inpatient bronchoscopy in reverse chronological
We report the case of a 55-year-old woman, former smoker since September 2012 (18 pack-years), with a history of allergic rhinoconjunctivitis and bronchial asthma. No infiltrates were observed in a chest X-ray performed in 2012. Skin prick tests showed sensitization to cat dander and grass and olive pollen.. She was admitted in 2014 for an acute episode of dyspnea, breath sounds, and non-productive cough without fever. Chest X-ray revealed an alveolointersticial pattern predominantly in the lung bases. Chest computed tomography showed bilateral patchy ground glass infiltrates. Bronchoscopy was performed with transbronchial biopsy and bronchoalveolar lavage: neutrophils: 11%; eosinophils: 85.9%; lymphocytes: 0.6%. Biopsy showed a histological pattern of pulmonary eosinophilia suggestive of chronic eosinophilic pneumonia (CEP), with a focal pattern typical of organizing pneumonia. Lung function tests highlighted a restrictive pattern with carbon monoxide diffusion changes: FVC 1830 - 59%; FEV1 ...
A P Greening, N C Dobson, P Nunn, A D M Rees; Bronchoalveolar Lavage in Fibrosing Alveolitis Assessment of Lymphocyte Proportions, Subtypes and Function. Clin Sci (Lond) 1 February 1984; 66 (2): 55P. doi: https://doi.org/10.1042/cs066055P_pt2. Download citation file:. ...
Lu et al prospectively studied 165 patients with culture-confirmed (bronchoalveolar lavage samples), ventilator-associated pneumonia (VAP) caused by either Pseudomonas aeruginosa or Acinetobacter baumannii.
Table II shows differential count for the three patients. There is increased neutrophil count after CPB with predominance of macrophages in both samples.. DISCUSSION. Inflammation may be understood as a protective response against the consequences of tissue aggression, organ dysfunction and cell necrosis 10, being a systemic process present even in the absence of systemic inflammatory response syndrome (SIRS) 11. However, if SIRS evolves to organ dysfunction, the inflammatory response may be noxious 10 with pulmonary function changes, renal failure, multiorgan failure syndrome (MOFS) and shock 11.. Postoperative pulmonary dysfunction is frequent and contributes to morbidity, mortality and hospitalization-related costs 12. The understanding of postoperative pulmonary dysfunction pathophysiology is critical for postoperative clinical pulmonary complication evaluation and the definition of therapeutic regimens.. Postoperative pulmonary dysfunction pathogenesis is associated to changes in gas ...
Case Report Our case is a 4 month old, Sudanese boy, residing in Qatar, who presented to our hospital with fever for two weeks. He remained febrile in the hospital for two weeks, despite receiving Intravenous antibiotics for presumed partially treated meningitis. He also received IVIG for the suspicion of atypical Kawasaki. Computerised tomography of the head and abdomen and bone marrow studies were also inconclusive. The mother subsequently revealed that a previous child had died during infancy with prolonged fever and no specific diagnosis. This alerted the treating physicians to the possibility of immunodeficiency. Our patient had received BCG vaccine at birth without any complications, and three gastric aspirate samples were negative for tuberculosis. A broncho-alveolar lavage was performed, despite the absence of any respiratory symptoms, to exclude any unusual organisms causing prolonged fever. The broncho-alveolar lavage revealed mycobacterium tuberculosis which was resistant to Isoniazid ...
TY - JOUR. T1 - Antigenic identification of chemotactic factor inactivator in normal human serum and bronchoalveolar lavage fluid. AU - Robbins, Richard A.. AU - Rasmussen, Julie K.. AU - Clayton, Marcia E.. AU - Gossman, Gail L.. AU - Kendall, Todd J.. AU - Rennard, Stephen I.. PY - 1987/9. Y1 - 1987/9. N2 - Several proteins have been described that can modulate the activity of the complement component C5a, a potent chemoattractant for neutrophils. One of these inhibitors has been termed chemotactic factor inactivator (CFI). We hypothesized that CFI was antigenically present in normal human serum and that antigenic levels would correlate with the ability of serum to inhibit C5a. To test this hypothesis, CFI was purified from normal human serum, antibodies to CFI were developed in rabbits, and these reagents were used to develop an enzyme-linked immunoadsorbent assay to measure CFI. Sera from 32 normal volunteers were assayed for CFI and found to contain 77 ± 29 μg/ml (range 17 to 137 μg/ml). ...
Hometown: Salt Lake City, Utah. Meg impressed immediately with her poise, intelligence, and initiative. Of the approximately 35 veterinary students and 15 undergraduate students that have worked in my lab over the past eight years, Meg is THE MOST responsible, organized, intellectually curious and astute student I have encountered.. Our lab is somewhat unique as it requires many basic science benchtop skills, but also animal handling and sample collection from horses. Meg has participated in blood and bronchoalveolar lavage sample collection and physical examinations on horses and foals in various projects, and immediately impressed me with her horse sense and handling skills as well as her ability to quickly grasp technical procedures. She also excels with benchtop skills, enthusiastically learning ELISAs, cell culture, neutrophil isolation, and free radical analytics assays. Meg works very well with or without direct supervision, and never fails to accomplish what is asked of her and more as a ...
Metagenomic technologies enable the study of microbial genetic material in human biomedical sample types such as stool, nasal, oral, urogenital, skin and bronchoalveolar lavage samples. In addition, environmental samples such as soil, water, air and biofilms can be also be analyzed. Metagenomic data can not only be used to examine healthy microbiomes and shed light on causes, effects, and future therapies for a variety of diseases, but it can also be useful to help understand the environments microbial biodiversity. QIAGEN provides next-generation sequencing technologies for metagenomics, as well as qPCR assays and arrays for verification of sequencing results and screening for specific bacterial species, virulence factor genes, and antibiotic resistance genes ...
TY - JOUR. T1 - Intratracheal administration of endotoxin and cytokines. T2 - VIII. LPS induces E-selectin expression; anti-E-selectin and soluble E-selectin inhibit acute inflammation. AU - Ulich, T. R.. AU - Howard, S. C.. AU - Remick, D. G.. AU - Yi, E. S.. AU - Collins, T.. AU - Guo, K.. AU - Yin, S.. AU - Keene, J. L.. AU - Schmuke, J. J.. AU - Steininger, C. N.. AU - Welply, J. K.. AU - Williams, J. H.. PY - 1994. Y1 - 1994. N2 - E-selectin is an inducible endothelial adhesion molecule that binds neutrophils. E-selectin mRNA is not constitutively detectable in the lungs of rats. Intratracheal injection of LPS induces pulmonary E-selectin mRNA expression at 2-4 h. Intratracheal injection of LPS followed at 2 and 4 h by intravenous injection of mouse F(ab) 2 or F(ab) anti-E-selectin monoclonal antibody inhibits the emigration of neutrophils into the bronchoalveolar space at 6 h by 50-70% . TNF and IL-6 bioactivity are not decreased in bronchoalveolar lavage fluid after treatment with ...
BioAssay record AID 423142 submitted by ChEMBL: Effect on pulmonary inflammation in BALB/c mouse lung assessed as change in peribronchiolar inflammation after 5 days by histopathological analysis.
COPD is an inflammatory disease of the airways characterised by increased levels of proinflammatory markers in lung tissue, and associated bronchial alveolar lavage (BAL) fluid and sputum. Increased sputum neutrophil levels have been correlated with disease progression and established as a primary biomarker of disease activity. Other biomarkers identified in sputum, such as CXCL8 (IL-8), club cell secretory protein (CC-16) and others, have been associated with disease activity, and in some studies correlate with disease progression.18 ,19. Previous studies have shown that both neutrophil and macrophage cell populations are increased in sputum and BAL samples taken from patients with COPD compared with those from disease-free patients,20 and that this increase is correlated with disease severity.21 ,22 Macrophages, in particular alveolar macrophages, are the most abundant type of immune cell found in the small airways, and they seem to accumulate in areas of tissue damage in the lungs.23 ,24 This ...
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Recent studies have revealed that bronchoalveolar lavage (BAL) fluid contains previously unappreciated communities of bacteria. In vitro and in vivo studies have shown that host inflammatory signals prompt bacteria to disperse from cell-associated biofilms and adopt a virulent free-living phenotype. The proportion of the lung microbiota that is cell-associated is unknown. Forty-six BAL specimens were obtained from lung transplant recipients and divided into two aliquots: whole BAL and acellular BAL, the latter processed with a low-speed, short-duration centrifugation step. Both aliquots were analyzed via bacterial 16S rRNA gene pyrosequencing. The BAL specimens represented a wide spectrum of lung health, ranging from healthy and asymptomatic to acutely infected. Bacterial signal was detected in 52% of acellular BAL aliquots, fewer than were detected in whole BAL (96%, p ≤ 0.0001). Detection of bacteria in acellular BAL was associated with indices of acute infection [BAL neutrophilia, high total
Principal Investigator:HAYASHI Seiji, Project Period (FY):1992 - 1993, Research Category:Grant-in-Aid for General Scientific Research (C), Research Field:Respiratory organ internal medicine
TY - JOUR. T1 - Cytotoxic drug-induced lung injury. AU - Snyder, Linda S.. AU - Hertz, Marshall I.. PY - 1988/9/1. Y1 - 1988/9/1. N2 - Cytotoxic drug-induced pulmonary disease is a major cause of morbidity and mortality in immunocompromised patients. It is estimated that 20% of all patients receiving cytotoxic drugs will develop a symptomatic pulmonary reaction. Therefore, the possibility of a toxic drug reaction must be considered in all patients developing pulmonary disease during or following treatment with these agents. Clinical manifestations of lung toxicity are nonspecific and parallel the signs and symptoms of diffuse lung infection and malignant disease in the chest. For this reason, the diagnosis of cytotoxic drug-induced lung damage is one of exclusion of the various opportunistic infections that afflict this patient population. This typically requires careful microbiologic studies of bronchoalveolar lavage (BAL) samples and lung tissue. The diagnosis of cytotoxic drug-induced lung ...
An alteration in the balance between a T-helper type 2 cell (Th2) response and a Th1 response may predispose to the development of bronchial asthma. Interleukin-18 (IL-18) has an ability to promote both Th1 and Th2 responses, depending on the surrounding cytokine environment. Reactive oxygen species (ROS) play a crucial role in the pathogenesis of airway inflammation and hyperresponsiveness. Recent studies have demonstrated that antioxidants are able to reduce airway inflammation and hyperreactivity in animal models of asthma. In this study, we used a C57BL/6 mouse model of allergic asthma to examine the effects of antioxidants on the regulation of IL-18 expression. Our present study with ovalbumin-induced murine model of asthma revealed that ROS production in cells from bronchoalveolar lavage fluids was increased and that administration of l-2-oxothiazolidine-4-carboxylic acid or α-lipoic acid reduced the increased levels of ROS, the increased expression of IL-18 protein and mRNA, airway ...
... u. ... .... _n of lung parenchyma by CD8+ cells ~hl!eOI~cut immunohistological observations f!CU:ii~J CD8+ infiltrate in the group of continued to ~ regularly exposed, and of CD4+ cells after 6 months in the lungs of were not. indicate that alveolitis in RP patients is a and its intensity might be modulated by to relevant antigens, amount of antigen of sensitization, thus explaining the BAL lymphocytosis observed in patients exposed to the specific antigens compared are noL Increased cells with supprcssor/cyin the lung of these patients is related to a local immunological response . These mechanisms may be relevant in the pathogenesis of HP.
Cytokines produced by activated TH2-type CD4+ T cells in the airway may contribute to late asthmatic responses by mechanisms that include eosinophil accumulation.
This is because co-receptor CD4 amplifies the signal produced for the T-cell receptor by recruiting lymphocyte protein kinase Lck, which consequently triggers a cascade of gatherings leading to T-cell activation. Curiously, DHLA didnt downregulate CD4 from the surface area of human peripheral blood mononuclear cells [a hundred forty five]. Instead or Along with CD4 downregulation, the immunomodulatory Houses of LA may perhaps include the upregulation of cAMP in T-cells and organic killer cells [146]. Mobile migration and neovascularization were being also inhibited by LA (86 μg/day in consuming h2o) in c57/black mice injected with Kaposis sarcoma inside of a matrigel sponge, in addition to in nude mice injected with KS cells [147]. In the mouse product of bronchial asthma, dietary LA considerably attenuated airway hyper-responsiveness, reduced the eosinophil depend among the bronchoalveolar lavage cells, and considerably improved pathologic lesion scores with the lungs [148]. LA inhibits ...
Results C57 mice demonstrated a higher total bronchoalveolar lavage (BAL) and BAL lymphocyte count at 3 and 7 days after intraperitoneal infection compared with BALB mice. There were no differences in BAL cytokine production; however, we were able to demonstrate differences in CMV DNA load in the lungs of BALB mice compared with that of C57 mice. In addition, there appeared to be increased whole-lung production of the TH2 cytokine IL-10 in the BALB mice versus the C57 mice. ...
The purpose of the study is two-fold: (1) to further evaluate the safety and potential immunogenicity of GLASSIA following IV administration via in-line filtration; and, (2) to assess the effects of GLASSIA augmentation therapy on the levels of A1PI and various biomarkers in the epithelial lining fluid (ELF) following intravenous (IV) administration at a dosage of 60 mg/kg Body weight (BW)/week active A1PI protein for 25 weeks in subjects with emphysema due to congenital A1PI deficiency ...
The differential cytology reflected mainly that described for healthy infants with lymphocyte counts at the upper range level. A positive association between BAL CD8+ lymphocytes and neutrophils and endobronchial reticular basement membrane was found. Detectable levels of pro-inflammatory cytokine proteins IL-1β, IL-17A, IL-18, IL-23, and IL-33 were found, whereas levels of Th2-type cytokine proteins were low. Frequent wheeze was the only clinical characteristic significantly related to detectable combined pro-inflammatory cytokine profile. Lung function did not correlate with any cytokine ...
Hung, Hung-Chang et al. Effectiveness of the BDProbeTec ET system for detection of Mycobacterium tuberculosis complex in sputum and bronchoalveolar lavage specimens. Braz J Infect Dis, June 2012, vol.16, no.3, p.242-249. ISSN 1413- ...
In our sample, male patients without a visible cause prevailed, as found in other studies (6). In respect to age, 68.2% were up to 3 years old, compatible with the period in which children are more susceptible to respiratory infections. Furthermore, infants and young children are more likely to develop chronic respiratory problems after infections because of the physiological characteristics of their airways (1). More than two thirds of the cases were of asthma and neuropathy since these diseases are usually associated to a more affected respiratory tract than that of the general population (1,2). In our study only one patient had oxygen desaturation this complication (4.5%), in a similar frequency reported in the literature (6-10 ...
Sample Processing is to collect target protein. The processing shall be done as gently as possible as protein denatures or degrades easily. It is also very important to store processed sample, especially avoiding repeated free-thaw cycles. Processed sample can be sub-packed and sealed. Expiry Time: a week at 4 degree; a month at -20 degree; two months at -80 degree. Just put sample in room until its temperature reaches room temperature before using the sample. It is forbidden to heat the sample to melt the sample.. Collect sample and then centrifuge it at 10000rpm for 10 minutes at 4 degree. Then collect supernatant and store it at -80 degree, avoiding repeated free-thaw cycles.. ...
BACKGROUND: The clinical utility of bronchoalveolar lavage fluid (BAL) cell analysis for the diagnosis and management of patients with interstitial lung disease (ILD) has been a subject of debate and controversy. The American Thoracic Society (ATS) sponsored a committee of international experts to examine all relevant literature on BAL in ILD and provide recommendations concerning the use of BAL in the diagnosis and management of patients with suspected ILD. PURPOSE: To provide recommendations for (1) the performance and processing of BAL and (2) the interpretation of BAL nucleated immune cell patterns and other BAL characteristics in patients with suspected ILD ...
meningitidis was developed and evaluated on BAL samples from adults with LRTI and a control group, and on CSF samples 3-MA datasheet from patients with meningitis. To establish the detection capacity of the Spn9802, the P6 and the ctrA assays, serial selleck inhibitor dilutions of target DNA with known concentration were repeatedly tested and the analytical sensitivity was 10-60 copies per PCR reaction for the Spn9802 assay, 3-30 copies per PCR reaction for the P6 assay and 5-50 copies per PCR reaction for the ctrA assay. As shown in Table 2 the analytical sensitivity. and quantification was not affected by using a combined mixture of reagents and a combined DNA standard (S. pneumoniae, H. influenzae and N. meningitidis) in single tubes. Table 2 Detection capacity of multiplex quantitative PCR. Oligos for a single target Oligos for three targets Δ Ct Δ copy number (log 10) DNA standard copy number of target DNA (number of reactions) Mean Ct value Mean measured copy number (log10) DNA standard ...
Alveolar resident memory T cells (T(RM)) comprise a currently uncharacterized mixture of cell subpopulations. The CD3(+)CD161(+) T cell subpopulation resides in the liver, intestine and skin, but it has the capacity for tissue migration; however, the presence of resident CD3(+)CD161(+) T cells in th …
Adults with CF given a bronchoscopy and bronchoalveolar lavage (BAL) as alternative to a sputum sample had no serious side effects in study.
This study examines the influence of Bu-Zhong-Yi-Qi-Tang (Hochuekkito; TJ-41) on the inflammatory response in alveolar macrophage in hyperglycemic mice. BALB/c mice (males, six to eight weeks old) were divided into three groups: A, B, and C. From the outset, group A and B were fed an ordinary diet, whereas TJ-41 was given to group C. After the initiation of these diets, intraperitoneal injection of streptozotocin (STZ), 250mg/g in mice of groups B and C. Four weeks after the start of these diets, blood-glucose levels were measured, and bronchoalveolar lavage (BAL) was performed. Alveolar macrophages were sampled, and those demonstrating blood-glucose levels of more than 200 mg/dL were selected for use. Toll-like receptor (TLR) ligands (TLR2: peptidoglycan [PGN], TLR4: LPS, TLR5: flagellin [FLG]) were employed to stimulate pulmonary-alveolar macrophages, and ELISA was used to measure TNF-a production. TLR expression on pulmonary-alveoli surface was evaluated on the basis of the emergence of each ...
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HIA has previously discussed the coding of bronchoscopy with bronchoalveolar lavage (BAL). The coding of procedures performed via bronchoscopy has become complicated in ICD-10-PCS. The coder must now think about the objective of the procedure to assign the correct root operation, the correct body part being addressed, and the correct approach and whether or not the intent of any removal of tissue or fluid was diagnostic.. Read More ...
Bronchoalveolar lavage (BAL) fluid; Nasopharyngeal swab. Please see Virology Specimen Collection Instructions at stanfordlab.com/esoteric/Virology.html ...
The integrity of the epithelial lining in intestines of cKO mice is maintained.(A) Tamoxifen-treated F/F;Cre (cKO, n = 11) and littermate F/F controls (Ctrl
entrations in their BAL fluids (Fig 1B). By contrast, subjects with asthma and healthy controls had similar mean concentrations of such other cytokines as
"Vitamin E Acetate in Bronchoalveolar-Lavage Fluid Associated with EVALI". New England Journal of Medicine. 382 (8): 697-705. ... "The Chemical Components of Electronic Cigarette Cartridges and Refill Fluids: Review of Analytical Methods". Nicotine & Tobacco ...
Samples from bronchoalveolar lavage fluid specimens returned the highest sensitivity. The authors argued that CT scans showed ...
... it is present in bronchoalveolar lavage fluid from cats undergoing experimentally induced asthma; it stimulates the local ...
"Osteopontin is increased in the bronchoalveolar lavage fluid and bronchial tissue of smoking asthmatics". Cytokine. 61 (3): 713 ...
The sample types were bronchoalveolar lavage (BAL) fluid samples (fluid samples collected from the lungs). The chemical was ... In May 2019, a 21-year-old was hospitalized for more than two weeks due to fluid in his lungs and was in a medically induced ... necessitating a medically induced coma and removal of fluid from the lungs. Vomiting, coughing up blood, and lipoid pneumonia ... My Lungs Were Full of Fluid'". People. Bojórquez, Kim (6 August 2019). "What Utah doctors are saying about vaping". Deseret ...
Allergen inhalation challenge of humans produces rises in the PGD2 levels in their Bronchoalveolar lavage fluids. Furthermore, ...
Wattiez R, Hermans C, Bernard A, Lesur O, Falmagne P (June 1999). "Human bronchoalveolar lavage fluid: two-dimensional gel ...
Typical inclusions called "Leventhal-Cole-Lillie bodies" can be seen within macrophages in BAL (bronchoalveolar lavage) fluid. ...
"De Novo Assembly of the Pneumocystis jirovecii Genome from a Single Bronchoalveolar Lavage Fluid Specimen from a Patient". mBio ... The genome of P. jirovecii has been sequenced from a bronchoalveolar lavage sample. The genome is small, low in G+C content, ... Fluid leaks into alveoli, producing an exudate seen as honeycomb/cotton candy appearance on hematoxylin and eosin-stained ... and those that have been detected in many mammals are only known from molecular sample detection from lung tissue or fluids, ...
"Novel Neutrophil-derived Proteins in Bronchoalveolar Lavage Fluid Indicate and Exaggerated Inflammatory Response in Pediatric ... "Costs of Bronchoalveolar Lavage-Directed Therapy in the First 5 Years of Life for Children with Cystic Fibrosis". Journal of ... "Effect of Bronchoalveolar Lavage-directed Therapy on Pseudomonas Aeruginosa Infection and Structural Lung Injury in Children ... "Safety of Bronchoalveolar Lavage in Young Children with Cystic Fibrosis". Pediatric Pulmonology. 43 (10): 965-72. doi:10.1002/ ...
Mizuki M, Komatsu H, Akiyama Y, Iwane S, Tsuda T (1999). "Inhibition of eosinophil activation in bronchoalveolar lavage fluid ...
Eosinophilic bronchitis is characterized by eosinophils in sputum and in bronchoalveolar lavage fluid without airway ... A cough is a sudden expulsion of air through the large breathing passages that can help clear them of fluids, irritants, ...
... bronchoalveolar lavage fluids, and conjunctival smears. Definitive diagnosis can also be achieved through fluorescein-tagged ...
On 24 December 2019, Wuhan Central Hospital sent a bronchoalveolar lavage fluid (BAL) sample from an unresolved clinical case ... Thus, the cornerstone of management of COVID-19 is supportive care, which includes treatment to relieve symptoms, fluid therapy ... proper intake of fluids, rest, and nasal breathing. Good personal hygiene and a healthy diet are also recommended. The U.S. ...
IL-13 expression has demonstrated to be increased in bronchoalveolar lavage (BAL) fluid and cells in patients with atopic mild ...
Neutrophils, beta-defensins, leukotrienes, and chemokines can also be detected in bronchoalveolar lavage fluid injected then ... has a normal neutrophil count detected in BAL fluid, and blood gas (an arterial blood test that measures the amount of oxygen ...
Similarly, increased levels of 5-oxo-ETE have been detected in the bronchoalveolar lavage fluid following the inhalation of ...
... can be measured in a number of biological fluids including bronchoalveolar lavage fluid (BALF) from asthmatic patients. " ... and limit markers of inflammation such as eosinophil counts in the peripheral blood and bronchoalveolar lavage fluid. This ...
... or when increased eosinophils are found in fluid obtained by a bronchoscopy (bronchoalveolar lavage fluid). Association with ... of white blood cells in fluid obtained by bronchoalveolar lavage. Other typical laboratory abnormalities include an elevated ... changes in multiple areas and fluid in the area surrounding the lungs on a chest X-ray, and eosinophils comprising more than 25 ...
Subsequent studies of bronchoalveolar lavage fluid from pediatric patients with asthma and also other severe chronic ... Webley WC, Salva PS, Andrzejewski C, Cirino F, West CA, Tilahun Y, Stuart ES (May 2005). "The bronchial lavage of pediatric ... C. pneumoniae has also been found in the cerebrospinal fluid of patients diagnosed with multiple sclerosis. C. pneumoniae ...
... bronchoalveolar lavage fluid of BAL) and human eosinophils, which are implicated in contributing to human asthma, metabolize ... "Lipidomic Profiling of Serum and Pancreatic Fluid in Chronic Pancreatitis". Pancreas. 41 (4): 518-22. doi:10.1097/MPA. ...
An unresolved clinical case sparks off the first scientific involvement: a bronchoalveolar lavage fluid (BAL) sample was sent ...
... body fluid compartments MeSH A12.207.200 - body water MeSH A12.207.234 - bronchoalveolar lavage fluid MeSH A12.207.270 - ... synovial fluid MeSH A12.207.515 - intracellular fluid MeSH A12.207.739 - nasal lavage fluid MeSH A12.207.927 - urine MeSH ... dentinal fluid MeSH A12.383.500 - gingival crevicular fluid MeSH A12.459.529 - meconium MeSH A12.459.764 - melena The list ... extracellular fluid MeSH A12.207.270.040 - aqueous humor MeSH A12.207.270.210 - cerebrospinal fluid MeSH A12.207.270.300 - ...
... urosepsis in a diabetic patient from whom the organism was also subsequently isolated from bronchoalveolar lavage fluid and a ... Swarming motility is the coordinated translocation of a bacterial population driven by flagellar rotation in film or on fluid ...
... showed a statistically significantly increase in bronchoalveolar lavage fluid levels after intratracheal ovalbumin challenge; ...
Elevated levels of IL-17A have been found in the sputum and in bronchoalveolar lavage fluid of patients with asthma and a ... Elevated concentration of IL-17 have been shown in vitreous fluid during proliferative diabetic retinopathy. Increased rates of ... "IL-17 in synovial fluids from patients with rheumatoid arthritis is a potent stimulator of osteoclastogenesis". The Journal of ...
... cerebrospinal fluid, bronchoalveolar lavage, biopsy) and include nucleic acids-based biomarkers such as gene mutations or ... body fluid, or biopsy biomarkers. Molecular biomarkers refer to non-imaging biomarkers that have biophysical properties, which ...
... and that 5-oxo-ETE is present in the bronchoalveolar lavage fluid from cats with experimentally induced asthma; these findings ... by bronchoalveolar macrophages isolated from human bronchoalveolar lavage washings; and by the human H295R adrenocortical cell ...
... bronchoalveolar lavage, lung biopsy, cerebrospinal fluid or brain biopsy specimens on selective agar allows differentiation ... Cryptococcal antigen testing from serum or cerebrospinal fluid is a useful preliminary test for cryptococcal infection, and has ...
... to the overall abnormal airway epithelial damage and there is a significant correlation between RL and bronchoalveolar lavage ... Moreover, the injury to epithelial cells handicaps the lung's ability to pump fluid out of airspaces. Fluid filled airspaces, ... Song W, Wei S, Zhou Y, Lazrak A, Liu G, Londino JD, Squadrito GL, Matalon S. (2010) Inhibition of lung fluid clearance and ... In the acute phase of ALI, there is increased permeability of this barrier and protein rich fluid leaks out of the capillaries ...
These tubes are used to drain air and fluid until the patient heals enough to take them out (usually a few days). Complications ... Bronchoalveolar lavage. Respiratory therapy/. intubation. *Artificial respiration. *CPR. *Hyperbaric medicine. *Decompression ... Furthermore, complications such as pleural effusion or hemothorax can occur if the chest tubes fail to drain the fluid around ... forming an airtight drainage system-are necessary to remove air and fluid from the pleural cavity, preventing the development ...
Bronchoalveolar lavage[edit]. Bronchoalveolar lavage (BAL) is a procedure whereby a small volume of fluid is put into the ... Cytopathology (examination under a microscope) of either a tracheal wash or a bronchoalveolar lavage sample can determine ... McKane, SA; Rose, RJ (10 June 2010). "Effects of exercise intensity and training on bronchoalveolar lavage cytology". Equine ... EIPH in the small airways may still be present and can be confirmed by a bronchoalveolar lavage. Impaired arterial blood gas ( ...
Protracted bacterial bronchitis is defined as a chronic productive cough with a positive bronchoalveolar lavage that resolves ... Tentative evidence supports honey and pelargonium to help with symptoms.[4] Getting plenty of rest and drinking enough fluids ...
Pleural effusion: accumulation of fluid in the pleural space *Chylothorax: a collection of lymphatic fluid in the pleural space ... Bronchoalveolar lavage. *Nasopharyngeal swab. *Sputum culture. Respiratory therapy/. intubation. *Artificial respiration. *CPR ... It is used to remove air (pneumothorax),[1] fluid (pleural effusion, blood, chyle), or pus (empyema) from the intrathoracic ... The first chamber allows fluid that is drained from the chest to be collected. The second chamber functions as a "water seal", ...
First the fluid is either transudate or exudate. A transudate is defined as pleural fluid to serum total protein ratio of less ... Bronchoalveolar lavage. Respiratory therapy/. intubation. *Artificial respiration. *CPR. *Hyperbaric medicine. *Decompression ... Normal pleural fluid pH is approximately 7.60. A pleural fluid pH below 7.30 with normal arterial blood pH has the same ... If a large amount of fluid is present, then this procedure can also be used therapeutically to remove that fluid and improve ...
"Exuberant plasmocytosis in bronchoalveolar lavage of the first patient requiring Extracorporeal Membrane Oxygenation for SARS- ... Fluid management) Ang layunin ay negatibong balanse ng 1/2-1L bawat araw ...
... whereby portable vacuum bottles are used to evacuate the pleural fluid. Routine evacuation keeps the pleura together, resulting ... Bronchoalveolar lavage. Respiratory therapy/. intubation. *Artificial respiration. *CPR. *Hyperbaric medicine. *Decompression ... between the parietal and the visceral layers of the pleura which closes off the space between them and prevents further fluid ...
It is not uncommon for surgical drains (see Drain (surgery)) to be required to remove blood or fluid from the surgical wound ... Bronchoalveolar lavage. Respiratory therapy/. intubation. *Artificial respiration. *CPR. *Hyperbaric medicine. *Decompression ... procedure for hydrocephalus was given by Al-Zahrawi who clearly describes the evacuation of superficial intracranial fluid in ...
... crusting of nasal fluids, difficult breathing, and whistling breathing. A turbinectomy may result in empty nose syndrome. ...
Pneumocystis jirovecii cysts from bronchoalveolar lavage, stained with Toluidine blue O stain. ... Notably, simple molecular detection of P. jirovecii in lung fluids does not mean that a person has PCP or infection by HIV. The ... can be definitively confirmed by histological identification of the causative organism in sputum or bronchoalveolar lavage ( ...
For PCR testing, the WHO recommends obtaining samples from the lower respiratory tract via bronchoalveolar lavage (BAL), sputum ... Wear an impermeable apron for some procedures with expected high fluid volumes that might penetrate the gown ...
Trough insulin levels in bronchoalveolar lavage following inhaled human insulin (Exubera®) in patients with diabetes mellitus. ... Metabolic hormones, apolipoproteins, adipokines, and cytokines in the alveolar lining fluid of healthy adults: ...
Some lacritin was reported in lung bronchoalveolar lavage and plasma. In lacrimal gland, polarized lacrimal acinar cells appear ... protein in the tear fluid is a potential biomarker of dry eye syndrome". PLOS ONE. 7 (12): e51979. Bibcode:2012PLoSO...751979A ... Lacritin is a glycoprotein of the human tear film, and to a lesser extent of saliva, lung lavage and plasma. It is mainly ... "Quantitative Analysis of N-linked Glycoproteins in Tear Fluid of Climatic Droplet Keratopathy by Glycopeptide Capture and iTRAQ ...
To view abnormalities of the airway To obtain tissue specimens of the inside the lungs by biopsy, bronchoalveolar lavage, or ... is a medical emergency and should be addressed with initiation of intravenous fluids and examination with rigid bronchoscopy. ...
A bronchoalveolar lavage can show an elevated (of at least 3.5) CD4/CD8 T cell ratio, which is indicative (but not proof) of ... In at least one study the induced sputum ratio of CD4/CD8 and level of TNF was correlated to those in the lavage fluid. A ... Cardiac sarcoidosis can cause fibrosis, granuloma formation, or the accumulation of fluid in the interstitium of the heart, or ...
... can be definitively confirmed by histological identification of the causative organism in sputum or bronchoalveolar lavage ( ... Notably, simple molecular detection of P. jirovecii in lung fluids does not mean that a person has PCP or infection by HIV. The ...
Detected Predominantly in Bronchoalveolar Lavage Fluid by Use of 16S rRNA Gene Sequencing Analysis Toshinori Kawanami, Kazumasa ... Usefulness of Two Aspergillus PCR Assays and Aspergillus Galactomannan and β-d-Glucan Testing of Bronchoalveolar Lavage Fluid ... A Comparison of Aspergillus and Mucorales PCR Testing of Different Bronchoalveolar Lavage Fluid Fractions from Patients with ... Positive Result of the Aspergillus Galactomannan Antigen Assay Using Bronchoalveolar Lavage Fluid from a Patient with an ...
Bronchoalveolar Lavage Fluid IFN-. Th17 Cells and Regulatory T Cells in Pulmonary Sarcoidosis. Anders Tøndell,1,2,3 Torolf Moen ... Bronchoalveolar lavage fluid cells, from 30 patients with sarcoidosis, 18 patients with other diffuse parenchymal lung diseases ... In sarcoidosis, increased Th17 cell fractions have been reported in bronchoalveolar lavage fluid, and elevated numbers of Th17 ...
Predictive Role of ADA in Bronchoalveolar Lavage Fluid in Making the Diagnosis of Pulmonary Tuberculosis, Fariba Binesh and ... Retracted: Predictive Role of ADA in Bronchoalveolar Lavage Fluid in Making the Diagnosis of Pulmonary Tuberculosis. Pulmonary ... Pulmonary Medicine, "Retracted: Predictive Role of ADA in Bronchoalveolar Lavage Fluid in Making the Diagnosis of Pulmonary ...
Bronchoscopy and bronchoalveolar lavage can be part of the clinical and diagnostic workup of e-cigarette, or vaping, product ... Bronchoscopy and bronchoalveolar lavage can be part of the clinical and diagnostic workup of e-cigarette, or vaping, product ... Evaluation of Bronchoalveolar Lavage Fluid from Patients in an Outbreak of E-cigarette, or Vaping, Product Use-Associated Lung ... Evaluation of Bronchoalveolar Lavage Fluid from Patients in an Outbreak of E-cigarette, or Vaping, Product Use-Associated Lung ...
... obtained through bronchoalveolar lavage in 15 individuals: five normal, five pigeon breeders, and five silicosis patients. The ... Analysis of fatty acids fractions of phospholipids and neutral lipids from bronchoalveolar lavage fluid (BALF) in patients with ... obtained through bronchoalveolar lavage in 15 individuals: five normal, five pigeon breeders, and five silicosis patients. The ...
In normal bronchoalveolar lavage fluid, lymphocytes typically account for fewer than 15% of the total cells. Measurements using ... Quantifying Lymphocytes in Bronchoalveolar Lavage Fluid Ware G. Kuschner, MD; James K. Brown, MD ... Quantifying Lymphocytes in Bronchoalveolar Lavage Fluid. Ann Intern Med. ;119:1050-1051. doi: 10.7326/0003-4819-119-10- ... This differential is not sufficient to explain the findings of a mean bronchoalveolar lavage lymphocyte percentage of 34.5% in ...
... ... We sought to determine if cellular and cytokine profiles in bronchoalveolar lavage fluid (BALF) from subjects with NEHI and FB ...
Quantifying Lymphocytes in Bronchoalveolar Lavage Fluid. Ann Intern Med. ;119:1050-1051. doi: 10.7326/0003-4819-119-10- ...
T-cell receptor variable region gene usage by CD4+ and CD8+ T cells in bronchoalveolar lavage fluid and peripheral blood of ... Finally, when analyzing TCR V gene usage by CD8+ bronchoalveolar lavage fluid and peripheral blood T cells, a normal V alpha ... T-cell receptor variable region gene usage by CD4+ and CD8+ T cells in bronchoalveolar lavage fluid and peripheral blood of ... T-cell receptor variable region gene usage by CD4+ and CD8+ T cells in bronchoalveolar lavage fluid and peripheral blood of ...
Galactomannan detection in broncho-alveolar lavage fluid for invasive aspergillosis in immunocompromised patients. To assess ... Galactomannan detection in broncho-alveolar lavage fluid for invasive aspergillosis in immunocompromised patients. Cochrane ...
... and to observe the difference in the cellular profiles of bronchoalveolar lavage (BAL) fluid with or without pneumoconiosis. ... Bronchoalveolar lavage in pneumoconiosis of coal miners. Cytologic aspects. Rev Fr Mal Respir1983;11:455-66. ... Bronchoalveolar lavage in pneumoconiosis of coal miners. Biochemical aspects. Rev Fr Mal Respir1983;11:417-26. ... BAL fluid was studied as previously described.3 The recovered BAL fluid was filtered through three layers of gauze and ...
We used bronchoalveolar lavage fluid (BALF) and peripheral blood (PB) for the analysis of cell profiles in patients with ... We used bronchoalveolar lavage fluid (BALF) and peripheral blood (PB) for the analysis of cell profiles in patients with ... T-cell subtypes in bronchoalveolar lavage fluid and in peripheral blood from patients with primary lung cancer Diagn Cytopathol ...
Fibroblast stimulating activity was measured in bronchoalveolar lavage (BAL) fluid to assess its relationship to the activity ... Fibroblast chemotactic response elicited by native bronchoalveolar lavage fluid from patients with fibrosing alveolitis. ... Fibroblast chemotactic response elicited by native bronchoalveolar lavage fluid from patients with fibrosing alveolitis. ... The procollagen III peptide level in BAL fluid served as a marker for collagen synthesis. RESULTS--Chemoattractant activity was ...
Bronchoalveolar lavage fluid cell counts in cryptogenic fibrosing alveolitis and their relation to therapy. ... Bronchoalveolar lavage fluid cell counts in cryptogenic fibrosing alveolitis and their relation to therapy. ... Significantly lower volumes of lavage fluid were recovered from the smokers with CFA (p , 0.001) and the fluid contained lower ... Bronchoalveolar lavage was used to sample inflammatory cells from the lungs of 51 patients with cryptogenic fibrosing ...
Comparison of IgA Levels in the Bronchoalveolar Lavage Fluid of Patients With and Without Antibiotic Therapy. The safety and ... Investigators will compare microbiota-dependent Ig production in the bronchoalveolar lavage fluid of patients with and without ... Comparison of IgA Levels in the Bronchoalveolar Lavage Fluid of Patients With and Without Antibiotic Therapy. ... IgA levels in the bronchoalveolar lavage fluid [ Time Frame: 2 weeks of ICU stay ]. ...
Increased levels of interleukin-18 in bronchoalveolar lavage fluid of patients with idiopathic nonspecific interstitial ... and cells in bronchoalveolar lavage fluid].. *Bronchoalveolar lavage fluid cellular characteristics, functional parameters and ... AdultAgedAntigensAntigens, NeoplasmBiomarkersBiopsyBronchoalveolar Lavage FluidCD4-CD8 RatioEnzyme-Linked Immunosorbent Assay ... Comparison of bronchoalveolar lavage fluids from nonspecific interstitial pneumonia and from ordinary interstitial pneumonia]. ...
Macrophage (AM) and Neutrophil (PMNL) Chemiliuminescence (CL) in Bronchoalveolar Lavage (BAL) Fluid C Ward; C Ward ... in Bronchoalveolar Lavage (BAL) Fluid. Clin Sci (Lond) 1 December 1987; 73 (s17): 33P-34P. doi: https://doi.org/10.1042/ ...
Asbestos bodies in bronchoalveolar lavage fluids of brake lining and asbestos cement workers. ... Asbestos bodies in bronchoalveolar lavage fluids of brake lining and asbestos cement workers. ... Examination of repeated bronchoalveolar lavage samples showed that the mechanisms of clearance of chrysotile fibres do not ... Asbestos body (AB) concentrations in bronchoalveolar lavage samples of 15 brake lining (BL) workers exposed only to chrysotile ...
Procoagulant (thromboplastin) activity in human bronchoalveolar lavage fluids is derived from alveolar macrophages. T Lyberg, B ... In the present study we found that both lung alveolar macrophages (LAM) and bronchoalveolar lavage fluids (BALF) from humans ... Procoagulant (thromboplastin) activity in human bronchoalveolar lavage fluids is derived from alveolar macrophages ... Procoagulant (thromboplastin) activity in human bronchoalveolar lavage fluids is derived from alveolar macrophages ...
... Thorax 41(4): 266-273. ... IgG and IgA concentrations were three times greater in lavage fluid from granite workers than the samples from non-industrial ... Immunoglobulin concentrations and lymphocyte counts were determined in bronchoalveolar fluid obtained from nine symptomless, ... in the lavage fluid whereas eight of nine (89%) granite workers had detectable IgM (chi 2 = 8, p less than 0.01). Lymphocyte ...
Elevation of tumour markers in serum and bronchoalveolar lavage fluid in pulmonary alveolar proteinosis. Y Hirakata, J ... It is unclear whether tumour markers are generally increased in the serum and bronchoalveolar lavage fluid (BALF) of patients ... Elevation of tumour markers in serum and bronchoalveolar lavage fluid in pulmonary alveolar proteinosis ... Elevation of tumour markers in serum and bronchoalveolar lavage fluid in pulmonary alveolar proteinosis ...
The extracellular vesicles derived from murine bronchoalveolar lavage fluid by both methods are quantified and characterized. ... Isolation of Extracellular Vesicles from Murine Bronchoalveolar Lavage Fluid Using an Ultrafiltration Centrifugation Technique ... Isolation of Extracellular Vesicles from Murine Bronchoalveolar Lavage Fluid Using an Ultrafiltration Centrifugation Technique ... to isolate extracellular vesicles from murine bronchoalveolar lavage fluid samples. ...
Cellular profiles of bronchoalveolar lavage fluid in 166 non-HIV-infected patients with Pneumocystis jirovecii pneumoniaa ... Cellular Profiles of Bronchoalveolar Lavage Fluid and Their Prognostic Significance for Non-HIV-Infected Patients with ... Cellular Profiles of Bronchoalveolar Lavage Fluid and Their Prognostic Significance for Non-HIV-Infected Patients with ... Cellular Profiles of Bronchoalveolar Lavage Fluid and Their Prognostic Significance for Non-HIV-Infected Patients with ...
... in bronchoalveolar lavage fluid of patients with chronic eosinophilic pneumonia. Download Prime PubMed App to iPhone, iPad, or ... AdultBronchoalveolar Lavage FluidCD4-Positive T-LymphocytesChemokine CXCL10Chemokine CXCL9Chemokines, CXCChronic Disease ... Elevated concentrations of liver-expressed chemokine/CC chemokine ligand 16 in bronchoalveolar lavage fluid from patients with ... Eosinophil accumulations are found in bronchoalveolar lavage fluid (BALF) from patients with chronic eosinophilic pneumonia ( ...
Complement factor H is elevated in bronchoalveolar lavage fluid and sputum from patients with lung cancer. Ruben - Pio, Javier ... Complement factor H is elevated in bronchoalveolar lavage fluid and sputum from patients with lung cancer ... Complement factor H is elevated in bronchoalveolar lavage fluid and sputum from patients with lung cancer ... Complement factor H is elevated in bronchoalveolar lavage fluid and sputum from patients with lung cancer ...
Complement Factor H Is Elevated in Bronchoalveolar Lavage Fluid and Sputum from Patients with Lung Cancer. Ruben Pio, Javier ... Evaluation of factor H in bronchoalveolar lavage fluids. Quantification of complement factor H was first done in samples from ... The content of complement factor H was also assessed in an independent series of bronchoalveolar lavage fluids from unselected ... Sensitivity and specificity of cytologic examination and the factor H and albumin tests in the bronchoalveolar lavage fluids ...
Bronchoalveolar Lavage Fluid Bronchoalveolar Lavage Chest Pain Dyspnea Endometriosis Erythrocytes Female Hemoptysis Macrophages ... Bronchoalveolar lavage(BAL) was performed when catamenial hemoptysis occurred. The BAL fluid showed many erythrocytes, ... A Case of Parenchymal Pulmonary Endometriosis Diagnosed by Cytology of Bronchoalveolar Lavage Fluid. ... BAL fluid Hemosiderin-laden macrophages Endometrial stromal cells MeSH Terms expand_less. expand_more. ...
Lower concentrations of uncorrected glutathione in the bronchoalveolar lavage fluid were found on the first day of life in ... These preliminary results suggest that glutathione accumulates in the lung epithelial lining fluid of preterm infants and that ...
"Cryptococcal antigen detection in broncho-alveolar lavage fluid, Medical Mycology" on DeepDyve, the largest online rental ... Cryptococcal antigen detection in broncho-alveolar lavage fluid. Cryptococcal antigen detection in broncho-alveolar lavage ... broncho-alveolar lavage fluid; CSF, cerebrospinal fluid; FLC, fluconazole; 5FC, flucytosine; HIV, human immunodeficiency virus ... broncho-alveolar lavage fluid; CSF, cerebrospinal fluid; FLC, fluconazole; 5FC, flucytosine; HIV, human immunodeficiency virus ...
Chemotactic activity of bronchoalveolar lavage fluid from children with recurrent obstructive bronchitis ... Identification of C5a des arg and a cationic eosinophil chemotactic activity in the bronchoalveolar lavage fluid of asthmatics ... Chemotactic monocyte and activation factor (MCAF/MCP1)in material from bronchoalveolar lavage fluid of patients with chronic ... Chemotactic activity in bronchoalveolar lavage fluid from patients with adult respiratory distress syndrome. American Review of ...
  • In this study, effects of C. sinensis methanolic extracts on bronchoalveolar lavage fluids (BALF) cells proliferation, inflammatory cytokines production, and genes expression were evaluated. (nih.gov)
  • Analysis of fatty acids fractions of phospholipids and neutral lipids from bronchoalveolar lavage fluid (BALF) in patients with occupational lung disease (OLD). (cdc.gov)
  • We sought to determine if cellular and cytokine profiles in bronchoalveolar lavage fluid (BALF) from subjects with NEHI and FB would differ from pediatric disease controls. (nih.gov)
  • We measured the serum and BAL fluid (BALF) levels of IL-18 and IFN-gamma in 22 patients with IIP (12 with UIP, 10 with NSIP) and 9 healthy volunteers. (unboundmedicine.com)
  • In the present study we found that both lung alveolar macrophages (LAM) and bronchoalveolar lavage fluids (BALF) from humans contained procoagulant activities. (ersjournals.com)
  • It is unclear whether tumour markers are generally increased in the serum and bronchoalveolar lavage fluid (BALF) of patients with pulmonary alveolar proteinosis (PAP). (ersjournals.com)
  • Eosinophil accumulations are found in bronchoalveolar lavage fluid (BALF) from patients with chronic eosinophilic pneumonia (CEP). (unboundmedicine.com)
  • In this study, we reviewed the performances of the CryAg detection (Premier EIA, Meridian) routinely performed in broncho-alveolar lavage fluid (BALF) during a 7-year period (2007-2013). (deepdyve.com)
  • Indeed, encapsulated yeasts may be missed due to the cellularity of the broncho-alveolar lavage fluid (BALF) or, by the contrary, some cells may be mistaken for yeasts leading to false-positive results. (deepdyve.com)
  • To further define the pulmonary inflammatory response, we conducted a proteome-based screen of bronchoalveolar lavage fluid (BALF) collected from young children with and without CF experiencing endobronchial infection. (edu.au)
  • Although studies have suggested that T cell subsets and cytokines in the Bronchoalveolar Lavage Fluid (BALF) may reflect the severity of pneumonia, the exact connection remains un-clarified. (alliedacademies.org)
  • Therefore, analysis of bronchoalveolar lavage fluid (BALF) to support stannosis might be of significant value. (pagepressjournals.org)
  • Objective: To investigate the pathogenesis of miRNA in asthma by measuring the microRNAs (miRNAs) levels in the bronchoalveolar lavage fluid (BALF) cells of children with asthma. (researchsquare.com)
  • Bronchoalveolar lavage fluid (BALF) is a rich milieu of macrophages that form the first line of defense against invading TB bacilli. (currenthivresearch.com)
  • Cell analysis and chemokines in bronchoalveolar lavage fluid (BALF) were analyzed at three days, one week, one month, three months, and six months after the instillation. (mdpi.com)
  • To further investigate this potential pathogenic relationship, we used ELISA-based techniques to assess anti-citrullinated HSP90 antibody profiles in bronchoalveolar lavage fluid (BALF) of patients with different stages of RA-ILD. (elsevier.com)
  • BACKGROUND: Bronchoalveolar lavage fluid (BALF) is an important diagnostic source to investigate molecular changes occurring in lung disorders. (unica.it)
  • We evaluated neutrophil involvement in subclinical pulmonary emphysema by measuring human neutrophil lipocalin (HNL) and two matrix metalloproteinases, gelatinase B (MMP-9) and neutrophil collagenase (MMP-8), in bronchoalveolar lavage fluid (BALF) from 65 community-based older volunteers. (elsevier.com)
  • The bronchoalveolar lavage fluid (BALF) from LPS- or bacteria-injected mice stimulated migration of tumor cells. (aacrjournals.org)
  • Quantification of haemosiderin-laden macrophages in bronchoalveolar lavage fluid (BALF) has been used to diagnose diffuse alveolar haemorrhage (DAH) but has not been assessed in patients with diffuse alveolar damage (DAD). (elsevier.com)
  • The aim of our study was to characterize the inflammatory response associated with airway hyperresponsiveness, and to determine whether the inflammatory cell response of bronchoalveolar lavage fluid (BALF) reflected changes in the bronchioles in this model. (elsevier.com)
  • The puppies were killed 3-4 days after inoculation, the lungs excised, the intermediate lobe lavaged, and BALF and the bronchiolar wall tissue examined for neutrophils and other inflammatory cells. (elsevier.com)
  • Here, we present a case of desquamative interstitial pneumonia (DIP) that was initially misdiagnosed as chronic eosinophilic pneumonia due to an increased number of eosinophils in the bronchoalveolar lavage fluid (BALF). (e-sciencecentral.org)
  • The analysis of cellular components of the bronchoalveolar lavage fluid (BALF) informs us about inflammatory and immune processes in the alveolar space. (biomedcentral.com)
  • Objectives -To determine effects of selectin inhibitor TBC1269 on neutrophil infiltration, and neutrophilassociated injury during pneumonia induced by Mannheimia haemolytica and concentration of antimicrobial anionic peptide (AAP) in bronchoalveolar lavage fluid (BALF) as well as antimicrobial activity of BALF from healthy (control) neonatal calves, neonatal calves with M haemolytica -induced pneumonia, neonatal calves with prior treatment with TBC1269, and adult cattle. (iastate.edu)
  • Bronchoalveolar lavage fluid (BALF) has higher sensitivity than sputum for detection of Mycobacterium tuberculosis (Mtb). (biomedcentral.com)
  • Then, nested PCR was carried out to detect trichomonads in bronchoalveolar lavage fluid (BALF). (beds.ac.uk)
  • To determine the prevalence of Trichomonas infection in the lungs, bronchoalveolar lavage fluids (BALF) from 115 cases were tested using nested PCR and microscopy. (beds.ac.uk)
  • The BAL fluid showed many erythrocytes, hemosiderin-laden macrophages, and sheets of endometrial stromal cells. (koreamed.org)
  • The expression of HLA-DR (Ia-like) antigens on human macrophages was investigated by analyses of cells from bronchoalveolar lavage fluid obtained from 12 patients with pulmonary sarcoidosis, six patients with extrinsic allergic alveolitis, nine patients with cryptogenic fibrosing alveolitis, 11 normal non-smokers, and 12 normal smokers. (uni-regensburg.de)
  • Previously a significant influx of exudate macrophages and persistence of bacteria on the bronchoalveolar surface were detected in these patients and a severe mucosal inflammation was observed bronchoscopically. (elsevier.com)
  • During August-October 2019, BAL fluid specimens were collected by clinical teams caring for hospitalized EVALI patients. (cdc.gov)
  • The quality of case-associated BAL specimens was assessed by measuring dipalmitoylphosphatidylcholine (DPPC), the principal phospholipid in naturally-occurring lung surfactant: the presence of acceptable levels of DPPC confirms that the lavage procedure recovered adequate pulmonary epithelial fluid. (cdc.gov)
  • Among the 27 BAL fluid specimens with sufficient volume for testing, all had measurable levels of DPPC. (cdc.gov)
  • In their recent article, Roberts and colleagues [1] reported cell counts and differentials in specimens obtained by bronchoalveolar lavage before and after radiotherapy in 17 women with breast cancer. (annals.org)
  • The authors did not, however, provide an explanation for the striking elevation in the mean percentage of lymphocytes in bronchoalveolar lavage specimens obtained before irradiation. (annals.org)
  • Bronchoscopic procedures for the diagnosis of lung cancer include cytologic examination of specimens from bronchial biopsy, bronchial brushing, and bronchial washing/bronchoalveolar lavage. (aacrjournals.org)
  • We describe a novel method to measure selected terpenes in BAL fluid by gas chromatography-tandem mass spectrometry (GC/MS/MS). Methods: α-Pinene, ß-pinene, ß-myrcene, 3-carene, and limonene were measured in BAL fluid specimens by headspace solid-phase microextraction/gas chromatography/tandem mass spectrometry. (cdc.gov)
  • Conclusions: We developed and validated a method to quantify selected terpenes in BAL fluid specimens using GC/MS/MS. The assay provided accurate and precise analyses of EVALI and non-EVALI BAL fluid specimens in support of CDC's EVALI response. (cdc.gov)
  • A PCR/ligase chain reaction technique was used to determine K-ras codon 12 mutations in a group of 52 bronchoalveolar lavage specimens from patients at risk of a second lung cancer. (elsevier.com)
  • Comparison between real-time PCR, conventional PCR and different staining techniques for diagnosing Pneumocystis jiroveci pneumonia from bronchoalveolar lavage specimens. (microbiologyresearch.org)
  • Samples from bronchoalveolar lavage fluid specimens returned the highest sensitivity. (wikipedia.org)
  • Cryptococcal antigen detection in broncho-alveolar lavage fluid 2018-08-01 00:00:00 Abstract Cryptococcal antigen (CryAg) testing in serum and CSF is a clue diagnostic tool for cryptococcosis. (deepdyve.com)
  • Pathologic changes induced by endotoxin inhalation include bronchospasm, airflow obstruction, recruitment of inflammatory cells, injury of the alveolar epithelium, and disruption of pulmonary capillary integrity leading to protein-rich fluid leak in the alveolar space ( 6 , 7 ). (jimmunol.org)
  • The fluids recovered from the first 20-ml aliquot and that from the next four aliquots were labeled bronchial and alveolar fluid, respectively. (northwestern.edu)
  • a similar trend was seen in the alveolar fluid (36.5 ± 9.4 vs 23.3 ± 3.0 μM/mg protein). (northwestern.edu)
  • Thus the granulomas in the BAL fluid probably reflect a high burden of alveolar wall granulomas and/or the removal of granulomas from proximal inflamed airways. (elsevier.com)
  • When using bronchoscopy for alveolar lavage, oral contact is avoided as much as possible. (beds.ac.uk)
  • Serum and bronchoalveolar lavage fluid levels of several tumor markers were measured in 71 patients with lung cancer, 36 patients with benign lung diseases and 100 healthy controls. (elsevier.com)
  • Fujii, M & Kiura, K 1988, ' Clinical evaluation of tumor markers in serum and bronchoalveolar lavage fluid in patients with lung cancer ', Japanese Journal of National Medical Services , vol. 42, no. 11, pp. 1012-1016. (elsevier.com)
  • Among idiopathic interstitial pneumonia (IIP), nonspecific interstitial pneumonia (NSIP) has an increased number of lymphocytes in bronchoalveolar lavage (BAL) fluid compared with usual interstitial pneumonia (UIP). (unboundmedicine.com)
  • The usefulness of bronchoalveolar lavage (BAL) fluid cellular analysis in non-human immunodeficiency virus (HIV)-infected patients with Pneumocystis jirovecii pneumonia (PCP) has not been adequately evaluated. (asm.org)
  • en] OBJECTIVE: To assess the predictive value of the endotoxin level in the bronchoalveolar lavage (BAL) and to propose to the clinician a guide in the diagnosis of gram-negative bacterial (GNB) pneumonia. (ac.be)
  • Levels of alpha-amylase in bronchoalveolar lavage (BAL) fluid can be used to detect pulmonary aspiration and predict bacterial pneumonia in critically ill, intubated adults, US researchers report. (medwirenews.com)
  • BAL fluid from 94 episodes of pneumonia in 81 patients was analyzed. (biomedcentral.com)
  • We aimed to evaluate the diagnostic value of bronchoalveolar lavage (BAL)-sTREM-1 concentration in neonatal ventilator-associated pneumonia (NVAP). (researchsquare.com)
  • In bronchoalveolar lavage fluid, specific metabolic profiles correlated with bacterial organisms known to play a role in the pathogenesis of pneumonia in HIV-infected individuals. (biomedcentral.com)
  • Pneumocystis carinii pneumonia: detection of parasites in sputum and bronchoalveolar lavage fluid by monoclonal antibodies. (microbiologyresearch.org)
  • Human metapneumovirus in bronchoalveolar lavage fluid of critically ill patients with suspected pneumonia. (maastrichtuniversity.nl)
  • Acute eosinophilic pneumonia due to toxocariasis with bronchoalveolar lavage findings. (nih.gov)
  • Results were validated in independent sets of bronchoalveolar lavage and sputum supernatants. (aacrjournals.org)
  • Detection of Mycobacterium tuberculosis in sputum, pleural and bronchoalveolar lavage fluid using DNA amplification of the MPB 64 protein coding gene and IS6110 insertion element. (nus.edu.sg)
  • The use of human bronchoalveolar lavage fluid and induced sputum to conduct pulmonary proteomics. (ac.be)
  • METHODS: Samples of bronchoalveolar lavage (BAL) fluid from 10 patients with emphysema and from control subjects matched for sex and current smoking status were assessed for collagenase, gelatinase, and NE activity. (bmj.com)
  • Asbestos body (AB) concentrations in bronchoalveolar lavage samples of 15 brake lining (BL) workers exposed only to chrysotile have been determined and compared with those from 44 asbestos cement (AC) workers extensively exposed to amphiboles. (bmj.com)
  • Examination of repeated bronchoalveolar lavage samples showed that the mechanisms of clearance of chrysotile fibres do not affect AB concentration for at least 10 months after cessation of exposure. (bmj.com)
  • IgG and IgA concentrations were three times greater in lavage fluid from granite workers than the samples from non-industrial controls (p less than 0.02). (eurekamag.com)
  • Here, we describe two extracellular vesicle isolation protocols, ultrafiltration centrifugation and ultracentrifugation with density gradient centrifugation, to isolate extracellular vesicles from murine bronchoalveolar lavage fluid samples. (jove.com)
  • Results: There was a significantly higher concentration of factor H in bronchoalveolar lavage samples from lung cancer patients. (aacrjournals.org)
  • Unexpectedly, levels of factor H in bronchoalveolar samples correlate with levels of albumin, suggesting that the cause of factor H accumulation in bronchial fluid is not its direct secretion from tumor cells but the exudation of plasma proteins to the bronchial lumen. (aacrjournals.org)
  • Boyden's method was used to assess the chemotactic activity of bronchoalveolar lavage (BAL) fluid samples from patients aged 1-6 yrs with recurrent obstructive bronchitis when signs of chronic mucosal inflammation were observed bronchoscopically. (eurekamag.com)
  • Novel Methods for the Analysis of Toxicants in Bronchoalveolar Lavage Fluid Samples from E-cigarette, or Vaping, Product Use-Associated Lung Injury (EVALI) Cases: Terpenes. (cdc.gov)
  • Bronchoalveolar lavage (BAL) fluid samples from 51 EVALI and 99 non-EVALI cases were analyzed for toxicants including terpenes. (cdc.gov)
  • The aim of this study was to evaluate the inter-laboratory agreement of three independently developed real-time PCR assays for the detection of P. jiroveci in bronchoalveolar lavage fluid samples. (microbiologyresearch.org)
  • We developed and performed profiling of 94 selected proteins in BAL fluid and serum samples obtained from newly diagnosed and non-treated patients with sarcoidosis. (scilifelab.se)
  • Bronchoalveolar lavage was performed in 22 children with recurrent obstructive bronchitis and the recovered lavage fluid samples were analysed for concentration of IgA, IgG, IgM, IgE and C3. (elsevier.com)
  • Bronchoalveolar lavage samples were subsequently analyzed for protein and phospholipid content. (biomedsearch.com)
  • Bronchoalveolar lavage fluid surfactant protein-A and surfactant protein-D are inversely related to inflammation in early cystic fibrosis. (mysciencework.com)
  • To quantify relationships among collectins, bacteria, and inflammation in early cystic fibrosis (CF) airway secretions, bronchoalveolar lavage fluids (BALFs) were collected from children undergoing clinically indicated bronchoscopy. (mysciencework.com)
  • BACKGROUND: Interleukin-8 (IL-8) and neutrophil elastase (NE) are commonly measured markers of inflammation in bronchoalveolar lavage (BAL) fluid from patients with cystic fibrosis. (edu.au)
  • METHODS: BAL fluid from 104 children with cystic fibrosis was assayed for IL-8 and NE after storage at 4 ° C for 7 days and -80 ° C for up to 6 years and compared with the initial assays performed soon after collection. (edu.au)
  • The proteins were measured in unconcentrated lavage fluid by means of a solid phase, enzyme linked immunosorbent assay. (eurekamag.com)
  • AbstractObjective To determine the concentration of proteins and phospholipids, markers of inflammatory reaction such as platelet-activating factor (PAF), and cell alterations in bronchoalveolar lavage (BAL) fluid during the evolution of the acute respiratory distress syndrome (ARDS). (semanticscholar.org)
  • Experimental FHF is associated with an increase of pulmonary permeability as indicated by increased proteins in BAL fluid, and surfactant alteration as indicated by the change in total BAL phospholipids. (biomedcentral.com)
  • Database of bronchoalveolar lavage fluid proteins. (ac.be)
  • This study aimed to find proteins in bronchoalveolar lavage (BAL) fluid that can be associated with the disease. (scilifelab.se)
  • The relative lavage fluid levels of immunoglobulins to albumin were significantly higher than in serum, indicating a local production of these proteins. (elsevier.com)
  • Immunoglobulin levels in bronchoalveolar lavage fluid of children with recurrent obstructive bronchitis. (elsevier.com)
  • Bronchoscopy and bronchoalveolar lavage † (BAL) can be part of the clinical and diagnostic workup of EVALI patients. (cdc.gov)
  • 2 Bronchoscopy and bronchoalveolar lavage (BAL) are not routinely performed in such patients. (bmj.com)
  • BAL was performed by fiberoptic bronchoscopy before, 4 hours and 7 hours after the surgical procedure respectively using 4 × 20 aliquots of normal saline after the first aspirated fluid. (biomedcentral.com)
  • Lemen, Richard J. / Bronchoalveolar lavage fluid cytology reflects airway inflammation in beagle puppies with acute bronchiolitis . (elsevier.com)
  • This differential is not sufficient to explain the findings of a mean bronchoalveolar lavage lymphocyte percentage of 34.5% in patients before irradiation. (annals.org)
  • Objective - To determine cytologic and microbiologic findings in bronchoalveolar lavage (BAL) fluid and Spo 2 values obtained during BAL in healthy rabbits. (elsevier.com)
  • Systemic LPS administration induces neutrophil sequestration into the pulmonary microvasculature without passage into the lung tissues and bronchoalveolar space ( 12 ), whereas neutrophil recruitment upon aerogenic LPS exposure occurs in all airway compartments ( 8 ). (jimmunol.org)
  • Pulmonary Medicine, "Retracted: Predictive Role of ADA in Bronchoalveolar Lavage Fluid in Making the Diagnosis of Pulmonary Tuberculosis," Pulmonary Medicine , vol. 2014, Article ID 426964, 1 pages, 2014. (hindawi.com)
  • It is expected that new approaches, such as the detection of molecular markers, could be used in the future to enhance the sensitivity of cytologic examination in the diagnosis of bronchial fluids. (aacrjournals.org)
  • Detection of Aspergillus DNA in BAL fluid by quantitative PCR (qPCR) offers the potential for earlier diagnosis and higher sensitivity. (biomedcentral.com)
  • The Aspergillus qPCR assay detected Aspergillus DNA in 76.9% of subjects with proven or probable IPA when the concentrated BAL fluid pellet fraction was used for diagnosis. (biomedcentral.com)
  • BAL-fluid sTREM-1 and serum C-reactive protein concentrations may be useful for the diagnosis of NVAP. (researchsquare.com)
  • A high degree of eosinophilia in the differential cell count of BAL fluid may eventually prove to be a useful clue in favor of such a diagnosis. (nih.gov)
  • F. Binesh and A. Halvani -Predictive role of ADA in bronchoalveolar lavage fluid in making the diagnosis of pulmonary tuberculosis,- Pulmonary Medicine, vol. 2013, Article ID 851518, 4 pages, 2013. (duhnnae.com)
  • It is concluded that occupational exposure to granite dust is associated with an increased proportion of lymphocytes and an increased concentration of immunoglobulin in lavage fluid that may reflect a subclinical immune inflammatory response. (eurekamag.com)
  • The objective of this study was to evaluate alterations of protein as a marker of permeability, phospholipid as a marker of surfactant alterations and PAF-acetylhydrolase (PAF-AcH) as inflammatory marker in bronchoalveolar lavage (BAL) fluid from pigs in an experimental surgical model of FHF. (biomedcentral.com)
  • Furthermore, it significantly reduced inflammatory cell counts and concentrations of cytokines such as MCP-1, MIP-1α, and MIP-2 in bronchoalveolar lavage fluid. (jimmunol.org)
  • To better characterize exposure among EVALI patients, CDC developed and validated isotope dilution mass spectrometry methods to analyze specific toxicants of concern and active compounds in case-associated BAL fluid. (cdc.gov)
  • These CDC analytic methods can identify vitamin E acetate, MCT oil (medium chain triglycerides), plant oils (long chain triglycerides), petroleum distillates (including mineral oil), diluent terpenes, cannabinoids, and nicotine in BAL fluid. (cdc.gov)
  • The extracellular vesicles derived from murine bronchoalveolar lavage fluid by both methods are quantified and characterized. (jove.com)
  • Methods: Complement factor H, a protein secreted by lung cancer cells, was quantified in a series of bronchoalveolar lavage supernatants from lung cancer patients and patients with non-malignant respiratory diseases. (aacrjournals.org)
  • METHODS: IL-9 protein was measured in bronchoalveolar lavage (BAL) fluid by means of ELISA and detected within the BAL cells by means of immunocytochemistry. (fraunhofer.de)
  • METHODS BAL fluids were collected from 51 patients with EVALI in 16 states and from 99 healthy participants who were part of an ongoing study of smoking involving nonsmokers, exclusive users of e-cigarettes or vaping products, and exclusive cigarette smokers that was initiated in 2015. (cdc.gov)
  • Using three different liquid chromatography coupled to tandem mass spectrometry targeted platforms (LC-MS/MS), we quantified a broad suite of lipid mediators including eicosanoids, sphingolipids and endocannabinoids in bronchoalveolar lavage (BAL) fluid from pulmonary sarcoidosis patients ( n = 41) and healthy controls ( n = 16). (springer.com)
  • A case of pulmonary sarcoidosis is presented in which cytologic analysis of bronchoalveolar (BAL) fluid showed intact granulomas. (elsevier.com)
  • Reference : Correlation between endotoxin level and bacterial count in bronchoalveolar lavage flu. (ac.be)
  • The receiver operating characteristic curve analysis showed that the Limulus assay still had a good discrimination power in the prediction of significant bacterial count in BAL fluid. (ac.be)
  • The common bacterial pathogens isolated from bronchoalveolar lavage (BAL) include Klebsiella pneumoniae , Acinetobacter baumanii , Pseudomonas aeruginosa and Coagulase negative Staphylococcus . (nepjol.info)
  • Targeted sequencing of the 16S rRNA gene was used to analyze the bacterial community structure and liquid chromatography-high-resolution mass spectrometry was used to detect features in bronchoalveolar lavage fluid. (biomedcentral.com)
  • In this report, we developed and tested a protocol to more efficiently recover bacterial DNA from low volumes of bronchoalveolar lavage fluid obtained from infants and adults. (telethonkids.org.au)
  • Furthermore, IL-9 protein and IL9 mRNA expression correlated with eosinophil numbers in BAL fluid. (fraunhofer.de)
  • Bronchoalveolar lavage fluid surfactant protein-A and surfacta. (mysciencework.com)
  • In this multicenter, controlled clinical trial of 60 preterm and 33 full-term neonates on mechanical ventilators, we measured concentrations of BAL-fluid and serum sTREM-1, serum C-reactive protein, and serum procalcitonin, as well as white blood cell count. (researchsquare.com)
  • BAL fluids were collected in ice-cold tubes (4°C). Total protein concentration, phospholipids and PAF-AcH were subsequently measured. (biomedcentral.com)
  • Total protein was found significantly higher in animals with FHF, while the protein concentration of the BAL fluid in sham-operated animals was not changed. (biomedcentral.com)
  • PMN accumulation in the lung (myeloperoxidase assay), bronchoalveolar lavage (BAL) fluid, and lung vascular permeability (protein content in BAL fluid) were assessed 6 h later. (physiology.org)
  • OBJECTIVES: To examine the effect of tumor necrosis factor-alpha (TNF-alpha) on pulmonary artery pressure and on total protein, phospholipid, lysophosphatidylcholine, phosphatidylcholine, phosphatidylinositol, and phosphatidylglycerol content in the bronchoalveolar lavage-accessible space of the isolated perfused rat lung, and to evaluate the role of the lung in the clearance of TNF-alpha from the perfusion medium in this model. (biomedsearch.com)
  • Using the BAL fluid, we performed isotope dilution mass spectrometry to measure several priority toxicants: vitamin E acetate, plant oils, medium-chain triglyceride oil, coconut oil, petroleum distillates, and diluent terpenes. (cdc.gov)
  • Vitamin E acetate was identified in BAL fluid obtained from 48 of 51 case patients (94%) in 16 states but not in such fluid obtained from the healthy comparator group. (cdc.gov)
  • In this review, we provide an overview of biomarkers for pediatric BPD and PH that have been identified in clinical studies using various biological fluids. (frontiersin.org)
  • Culture and histopathological examination of bronchoalveolar lavage (BAL) fluid are useful but have suboptimal sensitivity and in the case of culture may require several days for fungal growth to be evident. (biomedcentral.com)
  • The aim of the present study is to explore whether factor H levels are detectable in bronchial fluids of lung cancer patients and to evaluate its potential diagnostic usefulness. (aacrjournals.org)
  • Faculty Opinions recommendation of De novo assembly of the Pneumocystis jirovecii genome from a single bronchoalveolar lavage fluid specimen from a patient. (growkudos.com)
  • CONCLUSIONS: This study demonstrates, for the first time, the presence of increased levels of matrix metalloproteinases in the lungs of patients with emphysema and suggests that, in BAL fluid, collagenase activity may be a better indicator of the presence of emphysema than elastase. (bmj.com)
  • This finding suggests that the quantitative measurement of soluble CD138 in BAL fluid may reflect plasma cell infiltration and disease activity in the lungs of patients with MCD. (elsevier.com)
  • Subsequently, bleomycin-induced up-regulation of TGF-β in bronchoalveolar lavage fluid was significantly inhibited, and collagen deposition in the lungs was reduced. (jimmunol.org)
  • Tumor necrosis factor-alpha alters phospholipid content in the bronchoalveolar lavage-accessible space of isolated perfused rat lungs. (biomedsearch.com)
  • After a baseline sampling bronchoalveolar lavage, a reduction bronchoalveolar lavage was performed to establish a uniform amount of phospholipid in all lungs. (biomedsearch.com)
  • MWCNT exposure markedly increased A 2B adenosine receptor (A 2B AR) expression in the lungs and ADO level in bronchoalveolar lavage fluid, combined with elevation of blood neutrophils, collagen fiber deposition, and activation of myeloperoxidase (MPO) activity in the lungs. (springer.com)
  • Our results show that bronchoalveolar lavage fluids from subjects with lung cancer present a significantly higher expression of factor H than those from patients with nonmalignant pulmonary diseases. (aacrjournals.org)
  • In comparison with the chemotactic effect of casein, the activity of the fluid proved to be high for polymorphonuclear leukocytes (PMNs), but significantly low for mononuclear cells. (eurekamag.com)
  • When babies with and without CLD were compared at 10 days of age, bronchoalveolar lavage fluid from the babies with CLD had significantly increased IL-8 (114.0 vs 12.7 ng/ml), sICAM (19.0 vs 1.1 micrograms/ml), elastase (6.9 vs 0.9 micrograms/ml) and neutrophils (1.9 vs 0.4 x 10(9)/l). (bmj.com)
  • Further metabolite analysis demonstrated that four features were significantly overrepresented in the bronchoalveolar lavage (BAL) fluid of HIV-infected individuals compared to HIV-uninfected, including cystine, two complex carbohydrates, and 3,5-dibromo- l -tyrosine. (biomedcentral.com)
  • In sarcoidosis, increased Th17 cell fractions have been reported in bronchoalveolar lavage fluid, and elevated numbers of Th17 cells producing IFN- γ have been observed in peripheral blood. (hindawi.com)
  • The normal albumin concentration suggests that differences in permeability do not account for all of the increased immunoglobulin concentrations found in granite workers' lavage fluid and that some immunoglobulin is locally synthesised. (eurekamag.com)
  • A comparison of complement components/albumin ratios in BAL fluid with serum complement components/albumin ratios suggested a significant local production and/or concentration of these factors. (eurekamag.com)
  • The BAL-fluid sTREM-1 concentration was higher in NVAP cases than in non-NVAP cases, irrespective of extrapulmonary infection. (researchsquare.com)
  • Immunoglobulin concentrations and lymphocyte counts were determined in bronchoalveolar fluid obtained from nine symptomless, healthy, non-smoking granite workers (mean age 45.6, range 22-56 years) and nine normal, non-smoking, non industrial controls (mean age 22.8, range 21-32 years). (eurekamag.com)
  • In addition, cadherin 5 (CDH5) was found to correlate positively with lymphocyte cell numbers in BAL fluid. (scilifelab.se)
  • These preliminary results suggest that glutathione accumulates in the lung epithelial lining fluid of preterm infants and that a relative deficiency may predispose to lung injury. (bmj.com)
  • Increase in interleukin-8 and soluble intercellular adhesion molecule-1 in bronchoalveolar lavage fluid from premature infants who develop chronic lung disease. (bmj.com)
  • Interleukin-8 (IL-8), soluble intercellular adhesion molecule-1 (sICAM), elastase and neutrophils were assessed in bronchoalveolar lavage fluid from nine infants who developed chronic lung disease (CLD) after respiratory distress syndrome (RDS), seven who had recovered from RDS, and in four control infants. (bmj.com)
  • This is the first report to demonstrate a marked elevation of soluble CD138, despite the absence of plasma cells, in BAL fluid in an MCD patient with pulmonary involvement. (elsevier.com)
  • Detection of toxicants in bronchoalveolar-Iavage (BAL) fluid from patients with EVALI can provide direct information on exposure within the lung. (cdc.gov)
  • We created and characterized BAL fluid pools from non-EVALI individuals to determine assay accuracy, precision, linearity, limits of detection, and analytical specificity. (cdc.gov)
  • With the use of sensitive detection techniques, we report a higher prevalence of K-ras mutations in bronchoalveolar lavage than has been reported previously for lung cancer. (elsevier.com)
  • An investigation was conducted of the lipidic composition (phospholipids, neutral lipids and fatty acids) of the extracellular compartment of the pulmonary surfactant, obtained through bronchoalveolar lavage in 15 individuals: five normal, five pigeon breeders, and five silicosis patients. (cdc.gov)
  • Proteome analysis of bronchoalveolar lavage fluids reveals hos. (mysciencework.com)
  • We have compared the efficiency of the described method with that of a commercially available kit for microbiome analysis in body fluids. (telethonkids.org.au)
  • Analysis of bronchoalveolar lavage (BAL) fluid showed 64 percent eosinophils. (nih.gov)
  • Bronchoalveolar lavage (BAL) fluid is routinely used to assess for the presence of fungi at the site of pulmonary infection. (biomedcentral.com)
  • In fact, some molecular markers have already been shown to be differentially increased in bronchial fluids from lung cancer patients. (aacrjournals.org)
  • Bronchoalveolar lavage vs. bronchial brushing. (microbiologyresearch.org)
  • Mulshine, James L. / High frequency of K-ras codon 12 mutations in bronchoalveolar lavage fluid of patients at high risk for second primary lung cancer . (elsevier.com)
  • The mean levels of bronchoalveolar lavage fluid CEA, ferritin and β 2 MG in patients with lung cancer were statistically higher than those in patients with benign lung disease and healthy controls. (elsevier.com)
  • In this study, the levels of SP-D in sera and bronchoalveolar lavage (BAL) fluids of patients with lung diseases were determined by ELISA, using human recombinant SP-D as a standard. (nih.gov)
  • Lower concentrations of uncorrected glutathione in the bronchoalveolar lavage fluid were found on the first day of life in seven infants who subsequently developed chronic lung disease when compared with 27 infants who did not require supplemental oxygen at 36 weeks' postconceptional age. (bmj.com)
  • Conclusions and clinical relevance - Bronchoscopic BAL with 3 mL of saline solution provided adequate fluid recovery for microbiologic and cytologic examination from the caudal lung lobes. (elsevier.com)
  • We also performed repeated TCR V gene analyses on some HLA-DR3+ patients and found an association between the ratio bronchoalveolar lavage fluid/peripheral blood V alpha 2.3+ CD4+ T cells and clinical signs of disease activity. (pnas.org)
  • Finally, when analyzing TCR V gene usage by CD8+ bronchoalveolar lavage fluid and peripheral blood T cells, a normal V alpha 2.3 usage was found in all cases, but lung-restricted T-cell expansions using other TCR V gene segment products were identified. (pnas.org)
  • Patients with asthma generate increased amounts of reactive oxygen species (ROS) from peripheral blood cells and cells recovered by bronchoalveolar lavage (BAL). (northwestern.edu)
  • Retired underground miners were investigated to document alveolitis, and to observe the difference in the cellular profiles of bronchoalveolar lavage (BAL) fluid with or without pneumoconiosis. (bmj.com)
  • Alveolitis was not a representative feature of Turkish subjects with an occupational history of underground mining, and BAL fluid cellular profile did not seem to be different in miners with or without pneumoconiosis. (bmj.com)
  • We conducted a clinical trial to document alveolitis in miners, if any, and to observe the differences in the cellular profiles of BAL fluid of miners exposed to inorganic dust but who had not developed pneumoconiosis and those with different categories of pneumoconiosis. (bmj.com)
  • 02). CONCLUSIONS: a) TNF-alpha alters the amounts of phosphatidylcholine, phosphatidylinositol, and possibly phosphatidylglycerol present in the lavage-accessible space of the isolated perfused rat lung. (biomedsearch.com)
  • Bronchoalveolar lavage fluid glutathione in intubated premature infants. (bmj.com)
  • IL-8, sICAM, elastase and neutrophils in bronchoalveolar lavage fluid were increased in the CLD group, the differences being most pronounced at 10 days of age. (bmj.com)
  • Elevated levels of FN1 and CCL2 in bronchoalveolar lavage fluid from sarcoidosis patients. (scilifelab.se)
  • MEASUREMENTS AND MAIN RESULTS: Quantitative cultures for aerobic bacteria were performed directly from the fluid. (ac.be)