Washing liquid obtained from irrigation of the lung, including the BRONCHI and the PULMONARY ALVEOLI. It is generally used to assess biochemical, inflammatory, or infection status of the lung.
Abnormal intracellular inclusions, composed of denatured hemoglobin, found on the membrane of red blood cells. They are seen in thalassemias, enzymopathies, hemoglobinopathies, and after splenectomy.
A plant family of the order Eucommiales, subclass Hamamelidae, class Magnoliopsida (some botanists have classified this in the order Hamamelidales or Urticales). Eucomia is an elmlike tree of central and eastern China. Leaves are alternate; deciduous flowers are solitary and unisexual and lack petals and sepals. The male flowers have 6 to 10 stamens and female flowers have one ovary of two carpels, one of which aborts during development so the fruit (a dry, winged structure) contains only one seed. The latex is a source of RUBBER. Tochu tea is an aqueous extract of Eucommia ulmoides leaves and a popular beverage in Japan. (Mutat Res 1997 Jan 15;388(1):7-20).
The study of microorganisms such as fungi, bacteria, algae, archaea, and viruses.
A species of CARDIOVIRUS which contains three strains: Theiler's murine encephalomyelitis virus, Vilyuisk human encephalomyelitis virus, and Rat encephalomyelitis virus.
An autosomal recessive disorder that causes premature aging in adults, characterized by sclerodermal skin changes, cataracts, subcutaneous calcification, muscular atrophy, a tendency to diabetes mellitus, aged appearance of the face, baldness, and a high incidence of neoplastic disease.
It is a form of protection provided by law. In the United States this protection is granted to authors of original works of authorship, including literary, dramatic, musical, artistic, and certain other intellectual works. This protection is available to both published and unpublished works. (from Circular of the United States Copyright Office, 6/30/2008)
An idiopathic systemic inflammatory granulomatous disorder comprised of epithelioid and multinucleated giant cells with little necrosis. It usually invades the lungs with fibrosis and may also involve lymph nodes, skin, liver, spleen, eyes, phalangeal bones, and parotid glands.
Sarcoidosis affecting predominantly the lungs, the site most frequently involved and most commonly causing morbidity and mortality in sarcoidosis. Pulmonary sarcoidosis is characterized by sharply circumscribed granulomas in the alveolar, bronchial, and vascular walls, composed of tightly packed cells derived from the mononuclear phagocyte system. The clinical symptoms when present are dyspnea upon exertion, nonproductive cough, and wheezing. (Cecil Textbook of Medicine, 19th ed, p431)
Further or repeated use of equipment, instruments, devices, or materials. It includes additional use regardless of the original intent of the producer as to disposability or durability. It does not include the repeated use of fluids or solutions.
Washing out of the lungs with saline or mucolytic agents for diagnostic or therapeutic purposes. It is very useful in the diagnosis of diffuse pulmonary infiltrates in immunosuppressed patients.
The upper part of the trunk between the NECK and the ABDOMEN. It contains the chief organs of the circulatory and respiratory systems. (From Stedman, 25th ed)
T-cell receptors composed of CD3-associated alpha and beta polypeptide chains and expressed primarily in CD4+ or CD8+ T-cells. Unlike immunoglobulins, the alpha-beta T-cell receptors recognize antigens only when presented in association with major histocompatibility (MHC) molecules.
That region of the immunoglobulin molecule that varies in its amino acid sequence and composition, and comprises the binding site for a specific antigen. It is located at the N-terminus of the Fab fragment of the immunoglobulin. It includes hypervariable regions (COMPLEMENTARITY DETERMINING REGIONS) and framework regions.
Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
Intradermal injection of a heated (pasteurized) saline suspension of sarcoid tissue obtained from a sarcoid spleen or lymph node. In patients with active sarcoidosis a dusky red nodule develops slowly over the next few weeks at the injection site. Histologic examination, an essential part of the complete test, reveals sarcoid tissue.
A chronic systemic disease, primarily of the joints, marked by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Etiology is unknown, but autoimmune mechanisms have been implicated.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
A diffuse parenchymal lung disease caused by inhalation of dust and by tissue reaction to their presence. These inorganic, organic, particulate, or vaporized matters usually are inhaled by workers in their occupational environment, leading to the various forms (ASBESTOSIS; BYSSINOSIS; and others). Similar air pollution can also have deleterious effects on the general population.
Earth or other matter in fine, dry particles. (Random House Unabridged Dictionary, 2d ed)
A natural fuel formed by partial decomposition of vegetable matter under certain environmental conditions.
A group of interstitial lung diseases with no known etiology. There are several entities with varying patterns of inflammation and fibrosis. They are classified by their distinct clinical-radiological-pathological features and prognosis. They include IDIOPATHIC PULMONARY FIBROSIS; CRYPTOGENIC ORGANIZING PNEUMONIA; and others.
A diverse group of lung diseases that affect the lung parenchyma. They are characterized by an initial inflammation of PULMONARY ALVEOLI that extends to the interstitium and beyond leading to diffuse PULMONARY FIBROSIS. Interstitial lung diseases are classified by their etiology (known or unknown causes), and radiological-pathological features.
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
A process in which normal lung tissues are progressively replaced by FIBROBLASTS and COLLAGEN causing an irreversible loss of the ability to transfer oxygen into the bloodstream via PULMONARY ALVEOLI. Patients show progressive DYSPNEA finally resulting in death.
Analog or digital communications device in which the user has a wireless connection from a telephone to a nearby transmitter. It is termed cellular because the service area is divided into multiple "cells." As the user moves from one cell area to another, the call is transferred to the local transmitter.
Asbestos. Fibrous incombustible mineral composed of magnesium and calcium silicates with or without other elements. It is relatively inert chemically and used in thermal insulation and fireproofing. Inhalation of dust causes asbestosis and later lung and gastrointestinal neoplasms.
A type of asbestos that occurs in nature as the dihydrate of magnesium silicate. It exists in two forms: antigorite, a plated variety, and chrysotile, a fibrous variety. The latter makes up 95% of all asbestos products. (From Merck Index, 11th ed, p.893)
A class of asbestos that includes silicates of magnesium, iron, calcium, and sodium. The fibers are generally brittle and cannot be spun, but are more resistant to chemicals and heat than ASBESTOS, SERPENTINE. (From Hawley's Condensed Chemical Dictionary, 11th ed)
Asbestos, grunerite. A monoclinic amphibole form of asbestos having long fibers and a high iron content. It is used in insulation. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A form of pneumoconiosis caused by inhalation of asbestos fibers which elicit potent inflammatory responses in the parenchyma of the lung. The disease is characterized by interstitial fibrosis of the lung, varying from scattered sites to extensive scarring of the alveolar interstitium.
A lavender, acid-resistant asbestos.
Multi-subunit proteins which function in IMMUNITY. They are produced by B LYMPHOCYTES from the IMMUNOGLOBULIN GENES. They are comprised of two heavy (IMMUNOGLOBULIN HEAVY CHAINS) and two light chains (IMMUNOGLOBULIN LIGHT CHAINS) with additional ancillary polypeptide chains depending on their isoforms. The variety of isoforms include monomeric or polymeric forms, and transmembrane forms (B-CELL ANTIGEN RECEPTORS) or secreted forms (ANTIBODIES). They are divided by the amino acid sequence of their heavy chains into five classes (IMMUNOGLOBULIN A; IMMUNOGLOBULIN D; IMMUNOGLOBULIN E; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M) and various subclasses.
Represents 15-20% of the human serum immunoglobulins, mostly as the 4-chain polymer in humans or dimer in other mammals. Secretory IgA (IMMUNOGLOBULIN A, SECRETORY) is the main immunoglobulin in secretions.
A class of immunoglobulin bearing mu chains (IMMUNOGLOBULIN MU-CHAINS). IgM can fix COMPLEMENT. The name comes from its high molecular weight and originally being called a macroglobulin.
A form of pneumoconiosis resulting from inhalation of dust containing crystalline form of SILICON DIOXIDE, usually in the form of quartz. Amorphous silica is relatively nontoxic.
Transparent, tasteless crystals found in nature as agate, amethyst, chalcedony, cristobalite, flint, sand, QUARTZ, and tridymite. The compound is insoluble in water or acids except hydrofluoric acid.
Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.
Small polyhedral outpouchings along the walls of the alveolar sacs, alveolar ducts and terminal bronchioles through the walls of which gas exchange between alveolar air and pulmonary capillary blood takes place.
The susceptibility of CAPILLARIES, under conditions of increased stress, to leakage.
Pathological processes involving any part of the LUNG.
An important soluble regulator of the alternative pathway of complement activation (COMPLEMENT ACTIVATION PATHWAY, ALTERNATIVE). It is a 139-kDa glycoprotein expressed by the liver and secreted into the blood. It binds to COMPLEMENT C3B and makes iC3b (inactivated complement 3b) susceptible to cleavage by COMPLEMENT FACTOR I. Complement factor H also inhibits the association of C3b with COMPLEMENT FACTOR B to form the C3bB proenzyme, and promotes the dissociation of Bb from the C3bBb complex (COMPLEMENT C3 CONVERTASE, ALTERNATIVE PATHWAY).
Material coughed up from the lungs and expectorated via the mouth. It contains MUCUS, cellular debris, and microorganisms. It may also contain blood or pus.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
Diagnosis of the type and, when feasible, the cause of a pathologic process by means of microscopic study of cells in an exudate or other form of body fluid. (Stedman, 26th ed)
Tumors or cancer of the LUNG.

GM-CSF-deficient mice are susceptible to pulmonary group B streptococcal infection. (1/5496)

Granulocyte-macrophage colony-stimulating factor (GM-CSF) gene-targeted mice (GM-/-) cleared group B streptococcus (GBS) from the lungs more slowly than wild-type mice. Expression of GM-CSF in the respiratory epithelium of GM-/- mice improved bacterial clearance to levels greater than that in wild-type GM+/+ mice. Acute aerosolization of GM-CSF to GM+/+ mice significantly enhanced clearance of GBS at 24 hours. GBS infection was associated with increased neutrophilic infiltration in lungs of GM-/- mice, while macrophage infiltrates predominated in wild-type mice, suggesting an abnormality in macrophage clearance of bacteria in the absence of GM-CSF. While phagocytosis of GBS was unaltered, production of superoxide radicals and hydrogen peroxide was markedly deficient in macrophages from GM-/- mice. Lipid peroxidation, assessed by measuring the isoprostane 8-iso-PGF2alpha, was decreased in the lungs of GM-/- mice. GM-CSF plays an important role in GBS clearance in vivo, mediated in part by its role in enhancing superoxide and hydrogen peroxide production and bacterial killing by alveolar macrophages.  (+info)

Molecular detection of tumor cells in bronchoalveolar lavage fluid from patients with early stage lung cancer. (2/5496)

BACKGROUND: Conventional cytologic analysis of sputum is an insensitive test for the diagnosis of non-small-cell lung cancer (NSCLC). We have recently demonstrated that polymerase chain reaction (PCR)-based molecular methods are more sensitive than cytologic analysis in diagnosing bladder cancer. In this study, we examined whether molecular assays could identify cancer cells in bronchoalveolar lavage (BAL) fluid. METHODS: Tumor-specific oncogene mutations, CpG-island methylation status, and microsatellite alterations in the DNA of cells in BAL fluid from 50 consecutive patients with resectable (stages I through IIIa) NSCLC were assessed by use of four PCR-based techniques. RESULTS: Of 50 tumors, 28 contained a p53 mutation, and the identical mutation was detected with a plaque hybridization assay in the BAL fluid of 39% (11 of 28) of the corresponding patients. Eight of 19 adenocarcinomas contained a K-ras mutation, and the identical mutation was detected with a mutation ligation assay in the BAL fluid of 50% (four of eight) of the corresponding patients. The p16 gene was methylated in 19 of 50 tumors, and methylated p16 alleles were detected in the BAL fluid of 63% (12 of 19) of the corresponding patients. Microsatellite instability in at least one marker was detected with a panel of 15 markers frequently altered in NSCLC in 23 of 50 tumors; the identical alteration was detected in the BAL fluid of 14% (three of 22) of the corresponding patients. When all four techniques were used, mutations or microsatellite instability was detected in the paired BAL fluid of 23 (53%) of the 43 patients with tumors carrying a genetic alteration. CONCLUSION: Although still limited by sensitivity, molecular diagnostic strategies can detect the presence of neoplastic cells in the proximal airway of patients with surgically resectable NSCLC.  (+info)

Localization of a candidate surfactant convertase to type II cells, macrophages, and surfactant subfractions. (3/5496)

Pulmonary surfactant exists in the alveolus in several distinct subtypes that differ in their morphology, composition, and surface activity. Experiments by others have implicated a serine hydrolase in the production of the inactive small vesicular subtype of surfactant (N. J. Gross and R. M. Schultz. Biochim. Biophys. Acta 1044: 222-230, 1990). Our laboratory recently identified this enzyme in the rat as the serine carboxylesterase ES-2 [F. Barr, H. Clark, and S. Hawgood. Am. J. Physiol. 274 (Lung Cell. Mol. Physiol. 18): L404-L410, 1998]. In the present study, we determined the cellular sites of expression of ES-2 in rat lung using a digoxygenin-labeled ES-2 riboprobe. ES-2 mRNA was localized to type II cells and alveolar macrophages but not to Clara cells. Using a specific ES-2 antibody, we determined the protein distribution of ES-2 in the lung by immunohistochemistry, and it was found to be consistent with the sites of mRNA expression. Most of the ES-2 in rat bronchoalveolar lavage is in the surfactant-depleted supernatant, but ES-2 was also consistently localized to the small vesicular surfactant subfraction presumed to form as a consequence of conversion activity. These results are consistent with a role for endogenous lung ES-2 in surfactant metabolism.  (+info)

Expression of heat shock protein 72 by alveolar macrophages in hypersensitivity pneumonitis. (4/5496)

The current study was done to look at a possible role of heat shock proteins (HSPs) in hypersensitivity pneumonitis (HP). The specific aims were to determine whether there was a difference in the expression of HSP72 in alveolar macrophages (AMs) between mice challenged with HP antigen and saline-treated control mice and between AMs obtained by bronchoalveolar lavage from 18 patients with HP and 11 normal subjects. The expression of HSP72 was studied under basal conditions and under a mild heat shock. HSP72 expression by AMs in response to in vitro stimulation with Saccharopolyspora rectivirgula was lower in AMs of control mice than in those of HP animals. HSP72 was constitutively expressed in AMs of both normal and HP subjects. Densitometric ratios showed that AMs from normal subjects responded to heat shock with a 39 degrees C-to-37 degrees C ratio of 1.72 +/- 0.18 (mean +/- SE), and AMs from HP patients responded with a ratio of 1.16 +/- 0.16 (P = 0.0377). This decreased induction by additional stress of AMs could lead to an altered immunoregulatory activity and account for the inflammation seen in HP.  (+info)

A rapid polymerase chain reaction technique for detecting M tuberculosis in a variety of clinical specimens. (5/5496)

A rapid in-house polymerase chain reaction (PCR) assay is described for the direct detection of Mycobacterium tuberculosis complex in clinical material. Its performance is compared with two kit based systems. The results of the in-house assay were comparable with the commercial assays, detecting M tuberculosis in 100% of smear positive, culture positive samples. The in-house assay proved to be rapid, easy, and inexpensive to perform, and the inclusion of an internal inhibitor control permitted validation of the PCR results.  (+info)

Cigarette smoking decreases interleukin-8 secretion by human alveolar macrophages. (6/5496)

Cigarette smoking can impair pulmonary immune function, and hence influences the development of lung diseases. Interleukin-8 (IL-8) is a proinflammatory peptide and a potent chemotactic factor for neutrophils, and is produced by both immune and non-immune cells including monocytes and alveolar macrophages (AM). We investigated the effect of cigarette smoking on the secretion of IL-8 by human AM. The IL-8 concentration in bronchoalveolar lavage fluid (BALF) was much higher in smokers than in non-smokers (18.4 +/- 3.9 vs 4.1 +/- 1.0 pg ml-1; P < 0.005). However, spontaneous IL-8 secretion by cultured AM was lower in smokers than in non-smokers (46.8 +/- 12.7 vs 124.1 +/- 24.0 ng ml-1; P < 0.01). When stimulated with lipopolysaccharide (LPS), AM from smokers secreted significantly less IL-8 than those from non-smokers at all tested concentrations of LPS. In contrast, the amount of IL-8 secreted by peripheral blood monocytes with or without LPS stimulation was comparable in smokers and non-smokers. These observations indicate that smoking decreases IL-8 secretion by AM, which may modify or decrease the inflammatory response in the lung.  (+info)

Effect of hyperoxia on human macrophage cytokine response. (7/5496)

In the development of lung damage induced by oxidative stress, it has been proposed that changes in alveolar macrophages (AM) function with modifications in cytokine production may contribute to altered repair processes. To characterize the changes in profiles of cytokine production by macrophages exposed to oxidants, the effects of hyperoxia (95% O2) on interleukin (IL)-1 beta, IL-6, IL-8, and tumour necrosis factor-alpha (TNF-alpha) expression were studied. Experiments were first performed using AM obtained from control subjects and children with interstitial lung disease. Results showed that a 48 h O2 exposure was associated with two distinct patterns of response: a decrease in TNF-alpha, IL-1 beta and IL-6 expression, and an increase in IL-8. To complete these observations we used U937 cells that were exposed for various durations to hyperoxia. We confirmed that a 48 h O2 exposure led to similar changes with a decrease in TNF-alpha, IL-1 beta and IL-6 production and an increase in IL-8. Interestingly, this cytokine response was preceded during the first hours of O2 treatment by induction of TNF-alpha, IL-1 beta and IL-6. These data indicate that hyperoxia induces changes in the expression of macrophages inflammatory cytokines, and that these modifications appear to be influenced by the duration of O2 exposure.  (+info)

Pneumonia in febrile neutropenic patients and in bone marrow and blood stem-cell transplant recipients: use of high-resolution computed tomography. (8/5496)

PURPOSE: To obtain statistical data on the use of high-resolution computed tomography (HRCT) for early detection of pneumonia in febrile neutropenic patients with unknown focus of infection. MATERIALS AND METHODS: One hundred eighty-eight HRCT studies were performed prospectively in 112 neutropenic patients with fever of unknown origin persisting for more than 48 hours despite empiric antibiotic treatment. Fifty-four of these studies were performed in transplant recipients. All patients had normal chest roentgenograms. If pneumonia was detected by HRCT, guided bronchoalveolar lavage was recommended. Evidence of pneumonia on chest roentgenograms during follow-up and micro-organisms detected during follow-up were regarded as documentation of pneumonia. RESULTS: Of the 188 HRCT studies, 112 (60%) showed pneumonia and 76 were normal. Documentation of pneumonia was possible in 61 cases by chest roentgenography or micro-organism detection (54%) (P < 10(-6)). Sensitivity of HRCT was 87% (88% in transplant recipients), specificity was 57% (67%), and the negative predictive value was 88% (97%). A time gain of 5 days was achieved by the additional use of HRCT compared to an exclusive use of chest roentgenography. CONCLUSION: The high frequency of inflammatory pulmonary disease after a suspicious HRCT scan (> 50%) proves that pneumonia is not excluded by a normal chest roentgenogram. Given the significantly longer duration of febrile episodes in transplant recipients, HRCT findings are particularly relevant in this subgroup. Patients with normal HRCT scans, particularly transplant recipients, have a low risk of pneumonia during follow-up. All neutropenic patients with fever of unknown origin and normal chest roentgenograms should undergo HRCT.  (+info)

The objective of this study was to review the literature on bronchoalveolar lavage fluid cell profiles in healthy children and adolescents, as well as on the use of BAL as a diagnostic and follow-up tool for lung disease patients in this age bracket. To that end, we used the Medline database, compiling studies published between 1989 and 2009 employing the following MeSH descriptors (with Boolean operators) as search terms: bronchoalveolar lavage AND cytology OR cell AND child. In healthy children, the cell profile includes alveolar macrophages (> 80%), lymphocytes (approximately 10%), neutrophils (approximately 2%) and eosinophils (< 1%). The profile varies depending on the disease under study. The number of neutrophils is greater in wheezing children, especially in non-atopic children, as well as in those with pulmonary infectious and inflammatory profiles, including cystic fibrosis and interstitial lung disease. Eosinophil counts are elevated in children/adolescents with asthma and can reach ...
TY - JOUR. T1 - Increased angiostatin levels in bronchoalveolar lavage fluids from ARDS patients and from human volunteers after lung instillation of endotoxin. AU - Lucas, Rudolf. AU - Lijnen, H. Roger. AU - Suffredini, Anthony F.. AU - Pepper, Michael S.. AU - Steinberg, Kenneth P.. AU - Martin, Thomas R.. AU - Pugin, Jérôme. PY - 2002/1/1. Y1 - 2002/1/1. N2 - Acute respiratory distress syndrome (ARDS) is characterized by a disruption of the alveolar-capillary barrier, due to both an epithelial and an endothelial dysfunction. Whereas epithelial apoptosis seems to be mainly mediated by Fas ligand, the mediators of endothelial damage remain to be identified. Angiostatin, a powerful inhibitor of angiogenesis in vivo, also specifically induces apoptosis in endothelial cells. The concentration of various enzymes that cleave angiostatin from plasminogen was reported to be significantly increased in bronchalveolar lavage (BAL) fluids from patients with ARDS. Therefore, in this study, we ...
PAN Czytelnia Czasopism, Cytological evaluation of tracheal aspirate and broncho-alveolar lavage fluid in comparison to endoscopic assessment of lower airways in horses with recurrent airways obstruction or inflammatory airway disease - Polish Journal of Veterinary Sciences
The usefulness of bronchoalveolar lavage in the diagnosis of pulmonary infiltrates in the immunosuppressed patient was studied in 97 patients. In immunosuppressed patients, the available diagnostic procedures are often invasive and have variable yield and a potential for serious complications. Bronchoalveolar lavage had an overall diagnostic yield of 66% (61 of 92 diseases). It was most effective in the diagnosis of opportunistic infections, including infection with Pneumocystis carinii (18 of 22 cases), cytomegalovirus pneumonia (10 of 12 cases), fungal pneumonia (5 of 6 cases), and mycobacterial disease (4 of 5 cases). The technique was also helpful in confirming pulmonary hemorrhage (7 of 9 cases) but was less useful for diagnosing malignancy (10 of 22 cases) and drug-induced toxicity (6 of 15 cases). Findings of bronchoalveolar lavage were additive with those of transbronchial biopsies, brushings, and washings in the diagnosis of most of the diseases. The procedure was safe, even in ...
en] An increased production of NO* and peroxynitrite in lungs has been suspected during acute lung injury (ALI) in humans, and recent studies provided evidence for an alveolar production of nitrated compounds. We observed increased concentrations of nitrites/nitrates, nitrated proteins and markers of neutrophil degranulation (myeloperoxidase, elastase and lactoferrine) in the fluids recovered from bronchoalveolar lavage fluids (BALF) of patients with ALI and correlated these changes to the number of neutrophils and the severity of the ALI. We also observed that BALFs stimulated the DNA-binding activity of the nuclear transcription factor kappa B (NF-kappaB) as detected by electrophoretic mobility shift assay in human alveolar cells (A549) and monocytes (THP1). The level of activation of the NF-kappaB-binding activity was correlated to the concentration of nitrated proteins and myeloperoxidase. Furthermore, in vitro studies confirmed that NO*-derived species (peroxynitrite and nitrites) and the ...
Several components of cellular and humoral immunity were examined in bronchoalveolar lavage fluid and blood of 15 patients with the acquired immunodeficiency syndrome, and the results were compared to data from 25 healthy controls (including 5 asymptomatic homosexual men). Compared with that of controls, bronchoalveolar lavage fluid from patients tended to have more lymphocytes and significantly more neutrophils; a lower OKT4/OKT8 ratio, due to an increase in total OKT8 cells; and normal total OKT4 cell counts, despite a significant decrease in numbers of OKT4 cells in peripheral blood. Patients also had significantly more IgG-releasing cells and higher IgG levels than controls in lavage fluid. These data show that, in the lung lining fluid of patients with the acquired immunodeficiency syndrome, significant alterations in cellular and humoral immunity exist that differ in several important respects from immunity in controls and from corresponding changes in patients peripheral blood. ...
Fingerprint Dive into the research topics of Phenotypic expression of bronchoalveolar lavage cells in lung rejection and infection. Together they form a unique fingerprint. ...
Proteome analysis of bronchoalveolar lavage fluids reveals host and fungal proteins highly expressed during invasive pulmonary aspergillosis in mice and humans:
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
TY - JOUR. T1 - Novel approach for analysis of bronchoalveolar lavage fluid (BALF) using HPLC-QTOF-MS-based lipidomics. T2 - Lipid levels in asthmatics and corticosteroid-treated asthmatic patients. AU - Kang, Yun Pyo. AU - Lee, Won Jun. AU - Hong, Ji Yeon. AU - Lee, Sae Bom. AU - Park, Jeong Hill. AU - Kim, Donghak. AU - Park, Sunghyouk. AU - Park, Choon Sik. AU - Park, Sung Woo. AU - Kwon, Sung Won. PY - 2014/9/5. Y1 - 2014/9/5. N2 - To better understand the respiratory lipid phenotypes of asthma, we developed a novel method for lipid profiling of bronchoalveolar lavage fluid (BALF) using HPLC-QTOF-MS with an internal spectral library and high-throughput lipid-identifying software. The method was applied to BALF from 38 asthmatic patients (18 patients with nonsteroid treated bronchial asthma [NSBA] and 20 patients with steroid treated bronchial asthma [SBA]) and 13 healthy subjects (NC). We identified 69 lipids, which were categorized into one of six lipid classes: lysophosphatidylcholine ...
Patrolling Monocytes Promote the Pathogenesis of Early Lupus-Like Glomerulonephritis Researchers found that glomerulonephritis was driven by TLRs but, remarkably, proceeded independently of immune complexes. Rather, disease in three different mouse models and patients with systemic lupus erythematosus was characterized by glomerular accumulation of patrolling monocytes, a cell type with an emerging key function in vascular inflammation. [J Clin Invest] Full Article Lipopolysaccharide Inhalation Recruits Monocytes and Dendritic Cell Subsets to the Alveolar Airspace The authors used lipopolysaccharide inhalation to induce acute inflammation in healthy volunteers and examined the impact on bronchoalveolar lavage fluid and blood mononuclear phagocyte repertoire. Classical monocytes and two dendritic cell subsets were expanded in bronchoalveolar lavage fluid eight hours after lipopolysaccharide inhalation. [Nat Commun] Full Article FcγRIIb Differentially Regulates Pre-Immune and Germinal Center B ...
TY - JOUR. T1 - Increased levels of glutathione in bronchoalveolar lavage fluid from patients with asthma. AU - Smith, L. J.. AU - Houston, M.. AU - Anderson, J.. PY - 1993. Y1 - 1993. N2 - Patients with asthma generate increased amounts of reactive oxygen species (ROS) from peripheral blood cells and cells recovered by bronchoalveolar lavage (BAL). ROS produce many of the pathophysiologic changes associated with asthma and may contribute to its pathogenesis. Although antioxidant defenses inhibit the changes produced by ROS, no data are available on local antioxidant defenses in asthma. The present study was designed to begin to explore these defenses by measuring superoxide dismutase (SOD) and catalase activities and total glutathione (GSH) levels in BAL fluid from normal subjects and patients with mild asthma. Baseline pulmonary function and methacholine bronchoprovocation tests were performed on all subjects. BAL was achieved by instilling five 20-ml aliquots of phosphate-buffered saline in ...
Background: Cytologic examination of specimens obtained from the respiratory tract is a lung cancer diagnostic procedure with high specificity, but moderate sensitivity. The use of molecular biomarkers may enhance the sensitivity of cytologic examination in the detection of lung cancer. Methods: Complement factor H, a protein secreted by lung cancer cells, was quantified in a series of bronchoalveolar lavage supernatants from lung cancer patients and patients with non-malignant respiratory diseases. Albumin, total protein content, and hemoglobin were also analyzed. Results were validated in independent sets of bronchoalveolar lavage and sputum supernatants. Results: There was a significantly higher concentration of factor H in bronchoalveolar lavage samples from lung cancer patients. The sensitivity and specificity of the factor H test was 82% and 77%, respectively. These results were validated in an independent set of patients with nearly identical results. Furthermore, 70% and 45% of ...
The purpose of this study is to explore the correlations of interleukin 36 (IL-36) and Soluble B7-H3 (sB7-H3) levels in bronchoalveolar lavage fluid (BALF) with clinical characteristics and laboratory findings. A total of 35 children with M. pneumnoiae pneumonia (MPP) and 15 control subjects were enrolled. BALF concentrations of sB7-H3 and IL-36 were detected using enzyme-linked immunosorbent assays and clinical profiles of children with MPP were obtained. Children with MPP had significantly higher levels of sB7-H3 and IL-36 compared to control subjects (both P < 0.05). Meanwhile, children with pleural effusion had significantly higher levels of sB7-H3 and IL-36 compared to children without pleural effusion (both P < 0.05). BALF concentration of sB7-H3 was strongly associated with concentration of IL-36 (r = 0.796, P < 0.0001) and sB7-H3 was correlated with duration of fever (r = 0.427, P = 0.11) and length of stay (r = 0.345, P = 0.043). Both concentrations of sB7-H3 and IL-36 were significantly
Vitamin E acetate was universally found in bronchoalveolar lavage fluid samples from patients with vaping lung injury, providing the first direct evidence of vitamin E acetate at the site of injury.
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bronchoalveolar lavage,mechanical ventilation,platelet activating factor,surfactant,ventilator-associated lung injury,respiratory-distress syndrome,surfactant aggregate conversion,mechanical ventilation,pulmonary-surfactant,alveolar surfactant,dogs lungs,in-vitro,cytokines,clearance, ...
Cytokine Levels In Bronchoalveolar Lavage Fluid (Balf) In Patients With Systemic Sclerosis (SSc). In comparison to other diseases, SSc patients present a spe...
Bronchoscopy with bronchoalveolar lavage. Refractory pneumonitis. A 69-year-old man status post trauma, slightly prolonged respiratory failure status post tracheostomy, requires another bronchoscopy for further evaluation of refractory pneumonitis.
TY - JOUR. T1 - Bronchoalveolar lavage in the study of asthma. AU - Rennard, S. I.. AU - Thompson, A. B.. AU - Floreani, A. A.. PY - 1992. Y1 - 1992. UR - http://www.scopus.com/inward/record.url?scp=0026770648&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0026770648&partnerID=8YFLogxK. U2 - 10.1378/chest.102.1.1. DO - 10.1378/chest.102.1.1. M3 - Editorial. C2 - 1623734. AN - SCOPUS:0026770648. VL - 102. SP - 1. EP - 2. JO - Chest. JF - Chest. SN - 0012-3692. IS - 1. ER - ...
Cytology, Bronchoalveolar Lavage 2,ARUP Laboratories is a national reference laboratory and a worldwide leader in innovative laboratory research and development. ARUP offers an extensive test menu of highly complex and unique medical tests in clinical and anatomic pathology. Owned by the University of Utah, ARUP Laboratories client,medicine,medical supply,medical supplies,medical product
In this study we have analysed the phenotype of CD4+ and CD8+ T lymphocytes from PBL and BAL fluid, as well as peripheral blood monocytes and alveolar macrophages from patients with sarcoidosis. Cells were studied with respect to expression of various surface activation markers, co-stimulatory and adhesion molecules. In addition, we investigated differences between cells from patients with active and inactive disease.. Our results highlight the role of T cells in sarcoidosis as there was an increased expression by BAL lymphocytes of several of the molecules studied-for example, CD26, CD95 (Fas), and the activation markers CD69 and gp240. This is in agreement with previous studies investigating other activation markers such as IL-2R (CD25), very late activation antigen-1 (VLA-1), CD49a, CD49d, and HLA-DR.11-14 A role for the receptor-ligand adhesion molecule pair CD11a/CD18-CD54 is indicated by our demonstration of increased expression of CD11a on alveolar macrophages as well as on peripheral ...
bronchoalveolar definition: Adjective (comparative more bronchoalveolar, superlative most bronchoalveolar) 1. Relating to both the bronchi and the alvioli of the lungs...
The pathophysiological role of inflammation in lung diseases including asthma and chronic obstructive pulmonary disease is well established. Quantifying lung inflammation is relevant for the management of inflammatory airway diseases as it may indicate that pharmacological intervention is required before symptom onset and reduction in lung function. Moreover, monitoring of airway inflammation might be useful in the follow-up of patients with asthma and COPD, and for guiding pharmacological therapy. Quantification of pulmonary inflammation is currently based more on invasive methods including the analysis of broncho-alveolar lavage fluid, bronchoscopy, and bronchial biopsies, semi-invasive methods such as sputum induction, and the measurement of inflammatory biomarkers in plasma and urine, which are likely to reflect systemic rather than lung inflammation. Interest in the identification of non-invasive biomarkers for inflammatory airway diseases has been growing. Along these years less invasive ...
Pulmonary infection is normally a major reason behind mortality and morbidity as well as the magnitude from the lung inflammatory response correlates with affected person survival. and peptidoglycan (PGN) implemented intratracheally significantly elevated the amounts of neutrophils retrieved in the bronchoalveolar lavage liquid (BALF). F344 got approximately 10-flip even more neutrophils in the BALF weighed against DA (< 0.001) and higher BALF concentrations of total proteins tumor necrosis aspect-α and macrophage inflammatory proteins 2. LTA/PGN administration in DA×F344 congenic strains (with LTA/PGN created significantly higher degrees of keratinocyte-derived chemokine and macrophage inflammatory proteins 2 than alveolar macrophages from DA rats. The distinctions had been linked to differential mitogen-activated proteins kinase phosphorylation. We conclude the fact that factors adding to irritation could be site and problem dependent. A better knowledge of site-specific irritation can lead ...
Airborne Fe2O3 nanoparticles (NPs) with the mean diameter of 14 4 nm produced at spark ablation from 99.99% pure iron rods were fed into a nose-only exposure tower for rats exposed for 4 h a day, 5 days a week during 3, 6 or 10 months at a mean concentration of 1.14 0.01 mg/m3. Nanoparticles filtered out of the air exhausted from the exposure tower proved insoluble in de-ionized water but gradually dissolved in the cell free fluid supernatant produced by broncho-alveolar lavage and in the sterile bovine blood serum. The Fe2O3 content in lungs was measured by the Electron Paramagnetic Resonance (EPR) spectroscopy. NP retention in lungs and in brain was visualized with the Transmission Electron Microscopy (TEM). It was found a relatively low but significant pulmonary accumulation of Fe2O3, gradually increasing with time but tending to attain an equilibrium level. Besides, TEM-images showed nanoparticles retention within alveolocytes and the myelin sheaths of brain fibers associated with their ...
Jan Springer, P. Lewis White, Johanna Kessel, Imke Wieters, Daniel Teschner, Daniel Korczynski, Tobias Liebregts, Oliver A. Cornely, Stefan Schwartz, Thomas Elgeti, Lisa Meintker, Stefan W. Krause, Raquel B. Posso, Werner J. Heinz, Sandra Fuhrmann, Jörg Janne Vehreschild, Hermann Einsele, Volker Rickerts, Juergen Loeffler ...
The paper is concerned with the description of clinical, x-ray and morphological investigation of 123 bronchoalveolar cancer patients. Three types of this disease were defined: nodular (homogeneous and nonhomogeneous), pneumonia-like (infiltrative and infiltrative-nodular) and mixed (focal-disseminated, focal-nodular and focal-infiltrative). These types of bronchoalveolar cancer are most probably stages of the same tumor process. Clinical and x-ray signs of each type showed correlation with a morphological picture of a tumor. Shadow nonhomogeneity as one of the main x-ray signs of bronchoalveolar cancer was shown to be determined by the alveolar structure of a tumor, a tendency to the formation of small cavities, filled with viscous mucosa and air. Correct clinical and x-ray diagnosis in all types of bronchoalveolar cancer (before the use of the morphological methods) was established in 45.5% of the patients ...
The paper is concerned with the description of clinical, x-ray and morphological investigation of 123 bronchoalveolar cancer patients. Three types of this disease were defined: nodular (homogeneous and nonhomogeneous), pneumonia-like (infiltrative and infiltrative-nodular) and mixed (focal-disseminated, focal-nodular and focal-infiltrative). These types of bronchoalveolar cancer are most probably stages of the same tumor process. Clinical and x-ray signs of each type showed correlation with a morphological picture of a tumor. Shadow nonhomogeneity as one of the main x-ray signs of bronchoalveolar cancer was shown to be determined by the alveolar structure of a tumor, a tendency to the formation of small cavities, filled with viscous mucosa and air. Correct clinical and x-ray diagnosis in all types of bronchoalveolar cancer (before the use of the morphological methods) was established in 45.5% of the patients ...
To transport BAL samples from the field, pour samples into EDTA tubes or dilute 50:50 with grain alcohol or Vodka (100 Proof) and transport in clot tubes. Dont drink and drive! ...
This project will identify the characteristic immune cell profiles for two patient groups with allergic reactions with or without specific IgE in serum, also compared with healthy controls.
The figure above shows the overall detectability of the specified assay in a range of different mouse sample types. Detectability in each sample matrix is presented as the mean % of all samples included in the validation studies that could be measured above the limit of detection (LOD).. Dilution of samples may be necessary for some sample matrices other than plasma or serum to achieve optimal assay performance - the data shown above represents the values obtained at the optimal dilution identified in each case. Details regarding possible dilutions should be discussed with your Olink representative prior to commencing the study. The data is compiled from multiple studies comprising a range of healthy and disease samples, including samples from genetically modified mouse lines in some cases.. (Note: CSF = cerebrospinal fluid; BAL = bronchoalveolar lavage).. ...
Source: Adapted from the National Institutes of Health. What does the term lavage mean? The term lavage refers to washing out of an organ, especially the stomach. To find out more about this term, please search the news section of this website for related articles and information.. ...
Trimesulfin Balsámico is a medicine available in a number of countries worldwide. A list of US medications equivalent to Trimesulfin Balsámico is available on the Drugs.com website.
Confused on this one. Inpatient visist: When a Intensivist does a procedure of BAL Lavage and its done on both lungs. Is this report twice with CPT 3
differential cell counting in mixed cultures - posted in Tissue and Cell Culture: hello, i will be culturing neurons and macrophages together in a mixed culture. does anyone know of any methods for counting one cell type alone?
Also known as: bal, Bal., Bal, BaL, Ba.L, Ba-L. An, Ba-L, BA¶l, BAl, BAL., BAL, BA-L, B=Al, B.A.L., B.A.L, B. A. L., B-aL, B-AL ...
Le laveur de microplaques 405LS est la cinquième génération de laveurs BioTek, reconnus pour leur niveau de performance et durabilité. Sur ces nouveaux modèles, les vannes de sélection de solution de lavage sont intégrées et un nouveau design de peigne de lavage facilite le remplacement du peigne.
It was initially thought the use of chemical-grade silicone was limited to implants made during the past decade, but the TGA today said there were indications from European regulators the problem extended back to the late 1990s.
Data & statistics on Acute Lung Injury: MRNA expression of Interleukin-8 in bronchoalveolar lavage fluid cells before injury and after injury. NG, acute lung injury with normoglycemia group; HG, acute lung injury with hyperglycemia group; HG-VI, acute lung injury with hyperglycemia treated with intravenous insulin group; HG-AI, acute lung injury with hyperglycemia treated with aerosolized insulin group; IL-8, interleukin-8. Boxes extend ..., Particle-induced acute lung injury. Results obtained from analysis performed on bronchoalveolar lavage fluid samples recovered from animals at 24 h post-exposure. Animals were exposed by intratracheal instillation to: saline ( E); OFA100 ( C); OFA400 (P); ROFA#6 ( 1); or ARD ([). Various biomarkers of acute lung injury were assessed such as: A) edema/secretory activity (protein/mL); B) edema (albumin/mL); ..., Inhaltsverzeichnis Einleitung Hypothesen Theoretische Grundlagen der Hypothesen Patienten und Methodik Patienten Pneumonie Acute lung injury / Acute
TY - JOUR. T1 - Removal of bronchoalveolar cells augments the late eosinophilic response to segmental allergen challenge. AU - Hunt, L. W.. AU - Gleich, G. J.. AU - Kita, H.. AU - Weiler, D. A.. AU - Schroeder, D. R.. AU - Vuk Pavlovic, Z.. AU - Sur, Sanjiv. PY - 2002. Y1 - 2002. N2 - Background: In patients with quiescent asthma, macrophages are the most prevalent cells recovered by bronchoalveolar lavage (BAL). Through activation via their FcεRII receptors or by acting as antigen-presenting cells, macrophages could, in theory, promote the late airway response to allergen. Objective: In order to investigate the importance of macrophages and other airway luminal cells in inducing the late airway response, a novel washout experiment was designed. Methods: Five patients with ragweed-allergic asthma underwent bronchoscopy and segmental bronchial challenge with either normal saline or short ragweed extract in two segments of one lung. In a third segment of the opposite lung, 12 successive BALs ...
Invasive pulmonary aspergillosis (IPA) remains a major cause for morbidity and mortality in patients (pts) with hematologic malignancies. As culture-based methods only yield results in a minority of patients, using non-culture-based methods for detection of aspergillosis in clinical specimens becomes increasingly important. Analyzing bronchoalveolar lavage (BAL) samples with polymerase chain reaction (PCR) is promising, however, the influence of current antifungal drugs on the performance of this diagnostic tool remains controversial.. The aim of the trial is to elucidate on the performance of BAL PCR under antifungal treatment.. Patients with high risk of invasive aspergillosis and lung infiltrates are sampled via BAL, the sample is analyzed for fungal DNA by Apsergillus specific PCR. Clinical data including treatment data is assessed and evaluated. ...
TY - JOUR. T1 - Noninvasive quantification of airway inflammation following segmental allergen challenge with functional MR imaging. T2 - A proof of concept study. AU - Renne, Julius. AU - Hinrichs, Jan. AU - Schönfeld, Christian. AU - Gutberlet, Marcel. AU - Winkler, Carla. AU - Faulenbach, Cornelia. AU - Jakob, Peter. AU - Schaumann, Frank. AU - Krug, Norbert. AU - Wacker, Frank. AU - Hohlfeld, Jens M.. AU - Vogel-Claussen, Jens. PY - 2015/1/1. Y1 - 2015/1/1. N2 - Purpose: To evaluate oxygen-enhanced T1-mapping magnetic resonance (MR) imaging as a noninvasive method for visualization and quantification of regional inflammation after segmental allergen challenge in asthmatic patients compared with control subjects.Materials and After institutional review board approval, nine asthmatic Methods: and four healthy individuals gave written informed consent. MR imaging (1.5 T) was performed by using an inversion-recovery snapshot fast low-angle shot sequence before (0 hours) and 6 hours and 24 hours ...
Myeloid dendritic cells (DCs) are increased in the airway wall of patients with chronic obstructive pulmonary disease (COPD), and postulated to play a crucial role in COPD. However, DC phenotypes in COPD are poorly understood. Function-associated surface molecules on bronchoalveolar lavage fluid (BALF) DCs were analyzed using flow cytometry in current smokers with COPD, in former smokers with COPD and in never-smoking controls. Myeloid DCs of current smokers with COPD displayed a significantly increased expression of receptors for antigen recognition such as BDCA-1 or Langerin, as compared with never-smoking controls. In contrast, former smokers with COPD displayed a significantly decreased expression of these receptors, as compared with never-smoking controls. A significantly reduced expression of the maturation marker CD83 on myeloid DCs was found in current smokers with COPD, but not in former smokers with COPD. The chemokine receptor CCR5 on myeloid DCs, which is also important for the uptake and
Background. In acute respiratory distress syndrome or pneumonia, a procoagulant shift is observed in bronchoalveolar lavage fluid (BALF). The effect of a primarily extrapulmonary infection on coagulation and fibrinolysis in the pulmonary compartment is unclear. Methods. In 35 patients, 87 bronchoalveolar lavages were performed on the day of operation for secondary peritonitis (day 0) and on days 2 and 3 after surgery. Two noninfectious control groups were included: subjects undergoing bronchoalveolar lavage after elective surgery (n = 8) and those undergoing long-term mechanical ventilation (n = 10). Results. In BALF from patients with peritonitis, a tissue factor (TF)/factor VIIaâ€mediated activation of coagulation was shown (high levels of thrombin-antithrombin complexes). Levels of fibrinolysis activators decreased rapidly after day 0, whereas levels of inhibitors increased. The net effect was reduced fibrinolysis (plasminogen activator activity). The sequential comparison of plasma ...
We report the case of a 55-year-old woman, former smoker since September 2012 (18 pack-years), with a history of allergic rhinoconjunctivitis and bronchial asthma. No infiltrates were observed in a chest X-ray performed in 2012. Skin prick tests showed sensitization to cat dander and grass and olive pollen.. She was admitted in 2014 for an acute episode of dyspnea, breath sounds, and non-productive cough without fever. Chest X-ray revealed an alveolointersticial pattern predominantly in the lung bases. Chest computed tomography showed bilateral patchy ground glass infiltrates. Bronchoscopy was performed with transbronchial biopsy and bronchoalveolar lavage: neutrophils: 11%; eosinophils: 85.9%; lymphocytes: 0.6%. Biopsy showed a histological pattern of pulmonary eosinophilia suggestive of chronic eosinophilic pneumonia (CEP), with a focal pattern typical of organizing pneumonia. Lung function tests highlighted a restrictive pattern with carbon monoxide diffusion changes: FVC 1830 - 59%; FEV1 ...
A P Greening, N C Dobson, P Nunn, A D M Rees; Bronchoalveolar Lavage in Fibrosing Alveolitis Assessment of Lymphocyte Proportions, Subtypes and Function. Clin Sci (Lond) 1 February 1984; 66 (2): 55P. doi: https://doi.org/10.1042/cs066055P_pt2. Download citation file:. ...
Lu et al prospectively studied 165 patients with culture-confirmed (bronchoalveolar lavage samples), ventilator-associated pneumonia (VAP) caused by either Pseudomonas aeruginosa or Acinetobacter baumannii.
Table II shows differential count for the three patients. There is increased neutrophil count after CPB with predominance of macrophages in both samples.. DISCUSSION. Inflammation may be understood as a protective response against the consequences of tissue aggression, organ dysfunction and cell necrosis 10, being a systemic process present even in the absence of systemic inflammatory response syndrome (SIRS) 11. However, if SIRS evolves to organ dysfunction, the inflammatory response may be noxious 10 with pulmonary function changes, renal failure, multiorgan failure syndrome (MOFS) and shock 11.. Postoperative pulmonary dysfunction is frequent and contributes to morbidity, mortality and hospitalization-related costs 12. The understanding of postoperative pulmonary dysfunction pathophysiology is critical for postoperative clinical pulmonary complication evaluation and the definition of therapeutic regimens.. Postoperative pulmonary dysfunction pathogenesis is associated to changes in gas ...
Case Report Our case is a 4 month old, Sudanese boy, residing in Qatar, who presented to our hospital with fever for two weeks. He remained febrile in the hospital for two weeks, despite receiving Intravenous antibiotics for presumed partially treated meningitis. He also received IVIG for the suspicion of atypical Kawasaki. Computerised tomography of the head and abdomen and bone marrow studies were also inconclusive. The mother subsequently revealed that a previous child had died during infancy with prolonged fever and no specific diagnosis. This alerted the treating physicians to the possibility of immunodeficiency. Our patient had received BCG vaccine at birth without any complications, and three gastric aspirate samples were negative for tuberculosis. A broncho-alveolar lavage was performed, despite the absence of any respiratory symptoms, to exclude any unusual organisms causing prolonged fever. The broncho-alveolar lavage revealed mycobacterium tuberculosis which was resistant to Isoniazid ...
TY - JOUR. T1 - Antigenic identification of chemotactic factor inactivator in normal human serum and bronchoalveolar lavage fluid. AU - Robbins, Richard A.. AU - Rasmussen, Julie K.. AU - Clayton, Marcia E.. AU - Gossman, Gail L.. AU - Kendall, Todd J.. AU - Rennard, Stephen I.. PY - 1987/9. Y1 - 1987/9. N2 - Several proteins have been described that can modulate the activity of the complement component C5a, a potent chemoattractant for neutrophils. One of these inhibitors has been termed chemotactic factor inactivator (CFI). We hypothesized that CFI was antigenically present in normal human serum and that antigenic levels would correlate with the ability of serum to inhibit C5a. To test this hypothesis, CFI was purified from normal human serum, antibodies to CFI were developed in rabbits, and these reagents were used to develop an enzyme-linked immunoadsorbent assay to measure CFI. Sera from 32 normal volunteers were assayed for CFI and found to contain 77 ± 29 μg/ml (range 17 to 137 μg/ml). ...
Metagenomic technologies enable the study of microbial genetic material in human biomedical sample types such as stool, nasal, oral, urogenital, skin and bronchoalveolar lavage samples. In addition, environmental samples such as soil, water, air and biofilms can be also be analyzed. Metagenomic data can not only be used to examine healthy microbiomes and shed light on causes, effects, and future therapies for a variety of diseases, but it can also be useful to help understand the environments microbial biodiversity. QIAGEN provides next-generation sequencing technologies for metagenomics, as well as qPCR assays and arrays for verification of sequencing results and screening for specific bacterial species, virulence factor genes, and antibiotic resistance genes ...
TY - JOUR. T1 - Intratracheal administration of endotoxin and cytokines. T2 - VIII. LPS induces E-selectin expression; anti-E-selectin and soluble E-selectin inhibit acute inflammation. AU - Ulich, T. R.. AU - Howard, S. C.. AU - Remick, D. G.. AU - Yi, E. S.. AU - Collins, T.. AU - Guo, K.. AU - Yin, S.. AU - Keene, J. L.. AU - Schmuke, J. J.. AU - Steininger, C. N.. AU - Welply, J. K.. AU - Williams, J. H.. PY - 1994. Y1 - 1994. N2 - E-selectin is an inducible endothelial adhesion molecule that binds neutrophils. E-selectin mRNA is not constitutively detectable in the lungs of rats. Intratracheal injection of LPS induces pulmonary E-selectin mRNA expression at 2-4 h. Intratracheal injection of LPS followed at 2 and 4 h by intravenous injection of mouse F(ab) 2 or F(ab) anti-E-selectin monoclonal antibody inhibits the emigration of neutrophils into the bronchoalveolar space at 6 h by 50-70% . TNF and IL-6 bioactivity are not decreased in bronchoalveolar lavage fluid after treatment with ...
BioAssay record AID 423142 submitted by ChEMBL: Effect on pulmonary inflammation in BALB/c mouse lung assessed as change in peribronchiolar inflammation after 5 days by histopathological analysis.
Asthma is a chronic respiratory disease that is characterized by airway inflammation, bronchoconstriction and the influx of pro-inflammatory cells, mostly eosinophils in the lung tissue and bronchoalveolar space. Amongst the many physiopathological roles attributed to endothelins (ETs), one is to modulate pulmonary functions. It is established that Balb/c mice develop allergen-induced Th2-cytokine gene expression, airway inflammation and hyper-responsiveness whereas C57Bl/6 mice are much less reactive. In the present study, we investigated the roles of ETs in these two murine models of allergic asthma (AA). Mice were sensitized and challenged with either saline (S) and/or ovalbumin (O) over 6 weeks (groups S/S and O/O) and treated chronically with ABT-627 or its vehicle. Twenty-four hours after the last sensitization, challenged mice developed a marked airway inflammatory response characterized by the accumulation of total inflammatory cells (a 21-fold increase) in the bronchoalveolar space, ...
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Recent studies have revealed that bronchoalveolar lavage (BAL) fluid contains previously unappreciated communities of bacteria. In vitro and in vivo studies have shown that host inflammatory signals prompt bacteria to disperse from cell-associated biofilms and adopt a virulent free-living phenotype. The proportion of the lung microbiota that is cell-associated is unknown. Forty-six BAL specimens were obtained from lung transplant recipients and divided into two aliquots: whole BAL and acellular BAL, the latter processed with a low-speed, short-duration centrifugation step. Both aliquots were analyzed via bacterial 16S rRNA gene pyrosequencing. The BAL specimens represented a wide spectrum of lung health, ranging from healthy and asymptomatic to acutely infected. Bacterial signal was detected in 52% of acellular BAL aliquots, fewer than were detected in whole BAL (96%, p ≤ 0.0001). Detection of bacteria in acellular BAL was associated with indices of acute infection [BAL neutrophilia, high total
Principal Investigator:HAYASHI Seiji, Project Period (FY):1992 - 1993, Research Category:Grant-in-Aid for General Scientific Research (C), Research Field:Respiratory organ internal medicine
TY - JOUR. T1 - Cytotoxic drug-induced lung injury. AU - Snyder, Linda S.. AU - Hertz, Marshall I.. PY - 1988/9/1. Y1 - 1988/9/1. N2 - Cytotoxic drug-induced pulmonary disease is a major cause of morbidity and mortality in immunocompromised patients. It is estimated that 20% of all patients receiving cytotoxic drugs will develop a symptomatic pulmonary reaction. Therefore, the possibility of a toxic drug reaction must be considered in all patients developing pulmonary disease during or following treatment with these agents. Clinical manifestations of lung toxicity are nonspecific and parallel the signs and symptoms of diffuse lung infection and malignant disease in the chest. For this reason, the diagnosis of cytotoxic drug-induced lung damage is one of exclusion of the various opportunistic infections that afflict this patient population. This typically requires careful microbiologic studies of bronchoalveolar lavage (BAL) samples and lung tissue. The diagnosis of cytotoxic drug-induced lung ...
An alteration in the balance between a T-helper type 2 cell (Th2) response and a Th1 response may predispose to the development of bronchial asthma. Interleukin-18 (IL-18) has an ability to promote both Th1 and Th2 responses, depending on the surrounding cytokine environment. Reactive oxygen species (ROS) play a crucial role in the pathogenesis of airway inflammation and hyperresponsiveness. Recent studies have demonstrated that antioxidants are able to reduce airway inflammation and hyperreactivity in animal models of asthma. In this study, we used a C57BL/6 mouse model of allergic asthma to examine the effects of antioxidants on the regulation of IL-18 expression. Our present study with ovalbumin-induced murine model of asthma revealed that ROS production in cells from bronchoalveolar lavage fluids was increased and that administration of l-2-oxothiazolidine-4-carboxylic acid or α-lipoic acid reduced the increased levels of ROS, the increased expression of IL-18 protein and mRNA, airway ...
... u. ... .... _n of lung parenchyma by CD8+ cells ~hl!eOI~cut immunohistological observations f!CU:ii~J CD8+ infiltrate in the group of continued to ~ regularly exposed, and of CD4+ cells after 6 months in the lungs of were not. indicate that alveolitis in RP patients is a and its intensity might be modulated by to relevant antigens, amount of antigen of sensitization, thus explaining the BAL lymphocytosis observed in patients exposed to the specific antigens compared are noL Increased cells with supprcssor/cyin the lung of these patients is related to a local immunological response . These mechanisms may be relevant in the pathogenesis of HP.
Cytokines produced by activated TH2-type CD4+ T cells in the airway may contribute to late asthmatic responses by mechanisms that include eosinophil accumulation.
This is because co-receptor CD4 amplifies the signal produced for the T-cell receptor by recruiting lymphocyte protein kinase Lck, which consequently triggers a cascade of gatherings leading to T-cell activation. Curiously, DHLA didnt downregulate CD4 from the surface area of human peripheral blood mononuclear cells [a hundred forty five]. Instead or Along with CD4 downregulation, the immunomodulatory Houses of LA may perhaps include the upregulation of cAMP in T-cells and organic killer cells [146]. Mobile migration and neovascularization were being also inhibited by LA (86 μg/day in consuming h2o) in c57/black mice injected with Kaposis sarcoma inside of a matrigel sponge, in addition to in nude mice injected with KS cells [147]. In the mouse product of bronchial asthma, dietary LA considerably attenuated airway hyper-responsiveness, reduced the eosinophil depend among the bronchoalveolar lavage cells, and considerably improved pathologic lesion scores with the lungs [148]. LA inhibits ...
Results C57 mice demonstrated a higher total bronchoalveolar lavage (BAL) and BAL lymphocyte count at 3 and 7 days after intraperitoneal infection compared with BALB mice. There were no differences in BAL cytokine production; however, we were able to demonstrate differences in CMV DNA load in the lungs of BALB mice compared with that of C57 mice. In addition, there appeared to be increased whole-lung production of the TH2 cytokine IL-10 in the BALB mice versus the C57 mice. ...
The purpose of the study is two-fold: (1) to further evaluate the safety and potential immunogenicity of GLASSIA following IV administration via in-line filtration; and, (2) to assess the effects of GLASSIA augmentation therapy on the levels of A1PI and various biomarkers in the epithelial lining fluid (ELF) following intravenous (IV) administration at a dosage of 60 mg/kg Body weight (BW)/week active A1PI protein for 25 weeks in subjects with emphysema due to congenital A1PI deficiency ...
The differential cytology reflected mainly that described for healthy infants with lymphocyte counts at the upper range level. A positive association between BAL CD8+ lymphocytes and neutrophils and endobronchial reticular basement membrane was found. Detectable levels of pro-inflammatory cytokine proteins IL-1β, IL-17A, IL-18, IL-23, and IL-33 were found, whereas levels of Th2-type cytokine proteins were low. Frequent wheeze was the only clinical characteristic significantly related to detectable combined pro-inflammatory cytokine profile. Lung function did not correlate with any cytokine ...
Hung, Hung-Chang et al. Effectiveness of the BDProbeTec ET system for detection of Mycobacterium tuberculosis complex in sputum and bronchoalveolar lavage specimens. Braz J Infect Dis, June 2012, vol.16, no.3, p.242-249. ISSN 1413- ...
Sample Processing is to collect target protein. The processing shall be done as gently as possible as protein denatures or degrades easily. It is also very important to store processed sample, especially avoiding repeated free-thaw cycles. Processed sample can be sub-packed and sealed. Expiry Time: a week at 4 degree; a month at -20 degree; two months at -80 degree. Just put sample in room until its temperature reaches room temperature before using the sample. It is forbidden to heat the sample to melt the sample.. Collect sample and then centrifuge it at 10000rpm for 10 minutes at 4 degree. Then collect supernatant and store it at -80 degree, avoiding repeated free-thaw cycles.. ...
BACKGROUND: The clinical utility of bronchoalveolar lavage fluid (BAL) cell analysis for the diagnosis and management of patients with interstitial lung disease (ILD) has been a subject of debate and controversy. The American Thoracic Society (ATS) sponsored a committee of international experts to examine all relevant literature on BAL in ILD and provide recommendations concerning the use of BAL in the diagnosis and management of patients with suspected ILD. PURPOSE: To provide recommendations for (1) the performance and processing of BAL and (2) the interpretation of BAL nucleated immune cell patterns and other BAL characteristics in patients with suspected ILD ...
meningitidis was developed and evaluated on BAL samples from adults with LRTI and a control group, and on CSF samples 3-MA datasheet from patients with meningitis. To establish the detection capacity of the Spn9802, the P6 and the ctrA assays, serial selleck inhibitor dilutions of target DNA with known concentration were repeatedly tested and the analytical sensitivity was 10-60 copies per PCR reaction for the Spn9802 assay, 3-30 copies per PCR reaction for the P6 assay and 5-50 copies per PCR reaction for the ctrA assay. As shown in Table 2 the analytical sensitivity. and quantification was not affected by using a combined mixture of reagents and a combined DNA standard (S. pneumoniae, H. influenzae and N. meningitidis) in single tubes. Table 2 Detection capacity of multiplex quantitative PCR. Oligos for a single target Oligos for three targets Δ Ct Δ copy number (log 10) DNA standard copy number of target DNA (number of reactions) Mean Ct value Mean measured copy number (log10) DNA standard ...
Download Free Full-Text of an article EVALUATION OF DNA-CONTENT OF PERITONEAL LAVAGE CELLS BY FLOW CYTOMETRY IN PATIENTS WITH GASTERIC CANCER
Alveolar resident memory T cells (T(RM)) comprise a currently uncharacterized mixture of cell subpopulations. The CD3(+)CD161(+) T cell subpopulation resides in the liver, intestine and skin, but it has the capacity for tissue migration; however, the presence of resident CD3(+)CD161(+) T cells in th …
This study examines the influence of Bu-Zhong-Yi-Qi-Tang (Hochuekkito; TJ-41) on the inflammatory response in alveolar macrophage in hyperglycemic mice. BALB/c mice (males, six to eight weeks old) were divided into three groups: A, B, and C. From the outset, group A and B were fed an ordinary diet, whereas TJ-41 was given to group C. After the initiation of these diets, intraperitoneal injection of streptozotocin (STZ), 250mg/g in mice of groups B and C. Four weeks after the start of these diets, blood-glucose levels were measured, and bronchoalveolar lavage (BAL) was performed. Alveolar macrophages were sampled, and those demonstrating blood-glucose levels of more than 200 mg/dL were selected for use. Toll-like receptor (TLR) ligands (TLR2: peptidoglycan [PGN], TLR4: LPS, TLR5: flagellin [FLG]) were employed to stimulate pulmonary-alveolar macrophages, and ELISA was used to measure TNF-a production. TLR expression on pulmonary-alveoli surface was evaluated on the basis of the emergence of each ...
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Publikations-Datenbank der Fraunhofer Wissenschaftler und Institute: Aufsätze, Studien, Forschungsberichte, Konferenzbeiträge, Tagungsbände, Patente und Gebrauchsmuster
HIA has previously discussed the coding of bronchoscopy with bronchoalveolar lavage (BAL). The coding of procedures performed via bronchoscopy has become complicated in ICD-10-PCS. The coder must now think about the objective of the procedure to assign the correct root operation, the correct body part being addressed, and the correct approach and whether or not the intent of any removal of tissue or fluid was diagnostic.. Read More ...
Bronchoalveolar lavage (BAL) fluid; Nasopharyngeal swab. Please see Virology Specimen Collection Instructions at stanfordlab.com/esoteric/Virology.html ...
The integrity of the epithelial lining in intestines of cKO mice is maintained.(A) Tamoxifen-treated F/F;Cre (cKO, n = 11) and littermate F/F controls (Ctrl
entrations in their BAL fluids (Fig 1B). By contrast, subjects with asthma and healthy controls had similar mean concentrations of such other cytokines as
In the current version of the Excel Add-in, it is not possible to create a connection to a saved view or module with a total cell count of the
Lining up plans in San Cristóbal? Whether youre a local, new in town, or just passing through, youll be sure to find something on Eventbrite that piques your interest.
Hľadali ste balíky ktorých názvy obsahujú ruby-cocaine v sady bionic-updates, všetky sekcie a architektúry ppc64el. Ľutujeme hľadané kľúčové slová nevrátili žiadne výsledky. ...
Samples from bronchoalveolar lavage fluid specimens returned the highest sensitivity. The authors argued that CT scans showed ...
... diagnosis using routinely processed smears of bronchoalveolar lavage fluid". Journal of Clinical Pathology. 50 (12): 981-4. doi ... and ultrastructural findings in bronchoalveolar lavage fluid". Diagnostic Cytopathology. 24 (6): 389-95. doi:10.1002/dc.1086. ... The reported treatment of PAP using therapeutic bronchoalveolar lavage was in 1960 by Dr. Jose Ramirez-Rivera at the Veterans' ... Lung washings or tissue for histopathologic analysis are most commonly obtained using bronchoalveolar lavage and/or lung biopsy ...
Inhaled pentamidine is mainly deposited into the bronchoalveolar lavage fluid of the lungs. Metabolism: Pentamidine is ... When inhaled through a nebulizer, pentamidine accumulates in the bronchoalveolar fluid of the lungs at a higher concentration ... Ample fluids or intravenous hydration may prevent some nephrotoxicity. Liver: Elevated liver enzymes are associated with ... Additionally, pentamidine does not reach curative levels in the cerebrospinal fluid. It has a volume of distribution of 286- ...
... it is present in bronchoalveolar lavage fluid from cats undergoing experimentally induced asthma; it stimulates the local ...
The sample types were bronchoalveolar lavage (BAL) fluid samples (fluid samples collected from the lungs). The chemical was ... In May 2019, a 21-year-old was hospitalized for more than two weeks due to fluid in his lungs and was in a medically induced ... necessitating a medically induced coma and removal of fluid from the lungs. Vomiting, coughing up blood, and lipoid pneumonia ... My Lungs Were Full of Fluid'". People. Bojórquez, Kim (6 August 2019). "What Utah doctors are saying about vaping". Deseret ...
Mizuki M, Komatsu H, Akiyama Y, Iwane S, Tsuda T (1999). "Inhibition of eosinophil activation in bronchoalveolar lavage fluid ...
Eosinophilic bronchitis is characterized by eosinophils in sputum and in bronchoalveolar lavage fluid without airway ... A cough is a sudden expulsion of air through the large breathing passages that can help clear them of fluids, irritants, ...
If this is unavailable, a bronchoalveolar lavage can be done, and the bronchial wash fluid can be examined for eosinophils. The ... is also significantly increased in the bronchial wash fluid of eosinophilic bronchitis patients compared to asthmatic patients ...
The bronchoalveolar lavage (BAL) fluid of patients with idiopathic pulmonary fibrosis contains a higher concentration of ATP ... Following tissue injury in patients with Graft-versus-host disease (GVHD), ATP is released into the peritoneal fluid. It binds ...
Neutrophils, beta-defensins, leukotrienes, and chemokines can also be detected in bronchoalveolar lavage fluid injected then ... has a normal neutrophil count detected in BAL fluid, and blood gas (an arterial blood test that measures the amount of oxygen ...
... can be measured in a number of biological fluids including bronchoalveolar lavage fluid (BALF) from asthmatic patients. " ... and limit markers of inflammation such as eosinophil counts in the peripheral blood and bronchoalveolar lavage fluid. This ...
Subsequent studies of bronchoalveolar lavage fluid from pediatric patients with asthma and also other severe chronic ... Webley WC, Salva PS, Andrzejewski C, Cirino F, West CA, Tilahun Y, Stuart ES (May 2005). "The bronchial lavage of pediatric ... C. pneumoniae has also been found in the cerebrospinal fluid of patients diagnosed with multiple sclerosis. C. pneumoniae ...
... bronchoalveolar lavage fluid of BAL) and human eosinophils, which are implicated in contributing to human asthma, metabolize ... "Lipidomic Profiling of Serum and Pancreatic Fluid in Chronic Pancreatitis". Pancreas. 41 (4): 518-22. doi:10.1097/MPA. ...
... detection of L1 larvae in feces Microscope detection of eggs or larvae in the coughed up sputum or bronchoalveolar lavage fluid ...
... body fluid compartments MeSH A12.207.200 - body water MeSH A12.207.234 - bronchoalveolar lavage fluid MeSH A12.207.270 - ... synovial fluid MeSH A12.207.515 - intracellular fluid MeSH A12.207.739 - nasal lavage fluid MeSH A12.207.927 - urine MeSH ... dentinal fluid MeSH A12.383.500 - gingival crevicular fluid MeSH A12.459.529 - meconium MeSH A12.459.764 - melena The list ... extracellular fluid MeSH A12.207.270.040 - aqueous humor MeSH A12.207.270.210 - cerebrospinal fluid MeSH A12.207.270.300 - ...
... urosepsis in a diabetic patient from whom the organism was also subsequently isolated from bronchoalveolar lavage fluid and a ... Swarming motility is the coordinated translocation of a bacterial population driven by flagellar rotation in film or on fluid ...
... showed a statistically significantly increase in bronchoalveolar lavage fluid levels after intratracheal ovalbumin challenge; ...
... cerebrospinal fluid, bronchoalveolar lavage, biopsy) and include nucleic acids-based biomarkers such as gene mutations or ... body fluid, or biopsy biomarkers. Molecular biomarkers refer to non-imaging biomarkers that have biophysical properties, which ...
2015). "High-throughput sequencing of 16S rDNA amplicons characterizes bacterial composition in bronchoalveolar lavage fluid in ... Bronchoalveolar lavage (BAL) (also known as bronchoalveolar washing) is a diagnostic method of the lower respiratory system in ... "Therapeutic limited bronchoalveolar lavage with fiberoptic bronchoscopy as a bridging procedure prior to total lung lavage in a ... Bronchoalveolar lavage can be a more sensitive method of detection than nasal swabs in respiratory molecular diagnostics, as ...
Bronchoalveolar lavage (BAL) is a procedure whereby a small volume of fluid is put into the airways in order sample the cells ... Cytopathology (examination under a microscope) of either a tracheal wash or a bronchoalveolar lavage sample can determine ... McKane, SA; Rose, RJ (10 June 2010). "Effects of exercise intensity and training on bronchoalveolar lavage cytology". Equine ... EIPH in the small airways may still be present and can be confirmed by a bronchoalveolar lavage. Impaired arterial blood gas ( ...
Oudhuis GJ, Beuving J,et al.Soluble Triggering Receptor Expressed on Myeloid cells-1 in bronchoalveolar lavage fluid is not ... Diagnostic significance of increased bronchoalveolar lavage fluid eosinophils. Am Rev Respir Dis1990; 142: 642-647. PMID: ... bronchoalveolar lavage fluid.Am Rev Respir Dis. 1991 May;143(5 Pt 1):1121-9. PMID: 2024824. ... ശ്വാസനാളാന്തര സ്രവങ്ങൾ (endotracheal aspirates), ശ്വസനികാവായുകോശ ക്ഷാളനം (Bronchoalveloar Lavage; BAL) വഴി കിട്ടുന്ന ദ്രവം [98] ...
In suspected ventilator-associated pneumonia it has been suggested that bronchoscopy or bronchoalveolar lavage is necessary ... In case of pleural effusion, thoracentesis is performed for examination of pleural fluid. ...
A bronchoalveolar lavage can show an elevated (of at least 3.5) CD4/CD8 T cell ratio, which is indicative (but not proof) of ... In at least one study the induced sputum ratio of CD4/CD8 and level of TNF was correlated to those in the lavage fluid. A ... Cardiac sarcoidosis can cause fibrosis, granuloma formation, or the accumulation of fluid in the interstitium of the heart, or ...
These other tests may include basic blood work, blood cultures, and bronchoalveolar lavage.[citation needed] The clinical ... An x-ray that shows ARDS is necessary for diagnosis (fluid in the small air sacs (alveoli) in both lungs). In addition, a ... The presence of fluid means the person experiences a feeling similar to 'drowning'. Difficulties breathing can quickly progress ...
... a bronchoalveolar lavage might be taken by an experienced pneumologist. If no bacteria or fungi grow, the culture is negative. ... Sputum is a thick fluid produced in the lungs and in the adjacent airways. Normally, fresh morning sample is preferred for the ...
Comparison of Biomarkers in Exhaled Breath Condensate and Bronchoalveolar Lavage. Am. J. Respir. Crit. Care Med. 2007; 175(3): ... Condensate is extracted from the walls of the condensation tube using a plunger and swept into a fluid pool for analysis. EBC ... that is a problem with all methods of sampling the airway and lungs including sputum collection and bronchoalveolar lavage. ... Exhaled breath condensate reflects not only the composition of the airway lining fluid and alveoli. EBC may also mix with ...
Mycoplasma, Legionella, Streptococcus, and Chlamydia can also be detected using PCR techniques on bronchoalveolar lavage and ... a collection of fluid may form in the space that surrounds the lung. Occasionally, microorganisms will infect this fluid, ... If the fluid is sterile, it must be drained only if it is causing symptoms or remains unresolved. In rare circumstances, ... If the infected fluid is not drained, the infection may persist, because antibiotics do not penetrate well into the pleural ...
To view abnormalities of the airway To obtain tissue specimens of the inside the lungs by biopsy, bronchoalveolar lavage, or ... is a medical emergency and should be addressed with initiation of intravenous fluids and examination with rigid bronchoscopy. ...
Detected Predominantly in Bronchoalveolar Lavage Fluid by Use of 16S rRNA Gene Sequencing Analysis Toshinori Kawanami, Kazumasa ... Usefulness of Two Aspergillus PCR Assays and Aspergillus Galactomannan and β-d-Glucan Testing of Bronchoalveolar Lavage Fluid ... A Comparison of Aspergillus and Mucorales PCR Testing of Different Bronchoalveolar Lavage Fluid Fractions from Patients with ... Positive Result of the Aspergillus Galactomannan Antigen Assay Using Bronchoalveolar Lavage Fluid from a Patient with an ...
Bronchoalveolar Lavage Fluid IFN-. Th17 Cells and Regulatory T Cells in Pulmonary Sarcoidosis. Anders Tøndell,1,2,3 Torolf Moen ... Bronchoalveolar lavage fluid cells, from 30 patients with sarcoidosis, 18 patients with other diffuse parenchymal lung diseases ... In sarcoidosis, increased Th17 cell fractions have been reported in bronchoalveolar lavage fluid, and elevated numbers of Th17 ...
Regulation of bronchoalveolar lavage fluids cell function by the immunomodulatory agents from Cordyceps sinensis.. Kuo YC1, ... In this study, effects of C. sinensis methanolic extracts on bronchoalveolar lavage fluids (BALF) cells proliferation, ...
Predictive Role of ADA in Bronchoalveolar Lavage Fluid in Making the Diagnosis of Pulmonary Tuberculosis, Fariba Binesh and ... Retracted: Predictive Role of ADA in Bronchoalveolar Lavage Fluid in Making the Diagnosis of Pulmonary Tuberculosis. Pulmonary ... Pulmonary Medicine, "Retracted: Predictive Role of ADA in Bronchoalveolar Lavage Fluid in Making the Diagnosis of Pulmonary ...
Bronchoscopy and bronchoalveolar lavage can be part of the clinical and diagnostic workup of e-cigarette, or vaping, product ... Bronchoscopy and bronchoalveolar lavage can be part of the clinical and diagnostic workup of e-cigarette, or vaping, product ... Evaluation of Bronchoalveolar Lavage Fluid from Patients in an Outbreak of E-cigarette, or Vaping, Product Use-Associated Lung ... Evaluation of Bronchoalveolar Lavage Fluid from Patients in an Outbreak of E-cigarette, or Vaping, Product Use-Associated Lung ...
... obtained through bronchoalveolar lavage in 15 individuals: five normal, five pigeon breeders, and five silicosis patients. The ... Analysis of fatty acids fractions of phospholipids and neutral lipids from bronchoalveolar lavage fluid (BALF) in patients with ... obtained through bronchoalveolar lavage in 15 individuals: five normal, five pigeon breeders, and five silicosis patients. The ...
In normal bronchoalveolar lavage fluid, lymphocytes typically account for fewer than 15% of the total cells. Measurements using ... Quantifying Lymphocytes in Bronchoalveolar Lavage Fluid Ware G. Kuschner, MD; James K. Brown, MD ... Quantifying Lymphocytes in Bronchoalveolar Lavage Fluid. Ann Intern Med. ;119:1050-1051. doi: 10.7326/0003-4819-119-10- ... This differential is not sufficient to explain the findings of a mean bronchoalveolar lavage lymphocyte percentage of 34.5% in ...
... ... We sought to determine if cellular and cytokine profiles in bronchoalveolar lavage fluid (BALF) from subjects with NEHI and FB ...
Quantifying Lymphocytes in Bronchoalveolar Lavage Fluid. Ann Intern Med. ;119:1050-1051. doi: 10.7326/0003-4819-119-10- ...
Collagenase and fibronectin in bronchoalveolar lavage fluid in patients with sarcoidosis. Message subject: (Your Name) has ...
T-cell receptor variable region gene usage by CD4+ and CD8+ T cells in bronchoalveolar lavage fluid and peripheral blood of ... Finally, when analyzing TCR V gene usage by CD8+ bronchoalveolar lavage fluid and peripheral blood T cells, a normal V alpha ... T-cell receptor variable region gene usage by CD4+ and CD8+ T cells in bronchoalveolar lavage fluid and peripheral blood of ... T-cell receptor variable region gene usage by CD4+ and CD8+ T cells in bronchoalveolar lavage fluid and peripheral blood of ...
Galactomannan detection in broncho-alveolar lavage fluid for invasive aspergillosis in immunocompromised patients. To assess ... Galactomannan detection in broncho-alveolar lavage fluid for invasive aspergillosis in immunocompromised patients. Cochrane ...
Bronchoalveolar lavage fluid cells were obtained from 22 patients with sarcoidosis and from 10 normal control subjects and ... This study was designed to determine whether cell populations in bronchoalveolar lavage fluid represent a reflection of disease ... Relation between immunocytological features of bronchoalveolar lavage fluid and clinical indices in sarcoidosis. ... Relation between immunocytological features of bronchoalveolar lavage fluid and clinical indices in sarcoidosis. ...
... and to observe the difference in the cellular profiles of bronchoalveolar lavage (BAL) fluid with or without pneumoconiosis. ... Bronchoalveolar lavage in pneumoconiosis of coal miners. Cytologic aspects. Rev Fr Mal Respir1983;11:455-66. ... Bronchoalveolar lavage in pneumoconiosis of coal miners. Biochemical aspects. Rev Fr Mal Respir1983;11:417-26. ... BAL fluid was studied as previously described.3 The recovered BAL fluid was filtered through three layers of gauze and ...
Comparison of IgA Levels in the Bronchoalveolar Lavage Fluid of Patients With and Without Antibiotic Therapy. The safety and ... Investigators will compare microbiota-dependent Ig production in the bronchoalveolar lavage fluid of patients with and without ... Comparison of IgA Levels in the Bronchoalveolar Lavage Fluid of Patients With and Without Antibiotic Therapy. ... IgA levels in the bronchoalveolar lavage fluid [ Time Frame: 2 weeks of ICU stay ]. ...
Increased levels of interleukin-18 in bronchoalveolar lavage fluid of patients with idiopathic nonspecific interstitial ... and cells in bronchoalveolar lavage fluid].. *Bronchoalveolar lavage fluid cellular characteristics, functional parameters and ... AdultAgedAntigensAntigens, NeoplasmBiomarkersBiopsyBronchoalveolar Lavage FluidCD4-CD8 RatioEnzyme-Linked Immunosorbent Assay ... Comparison of bronchoalveolar lavage fluids from nonspecific interstitial pneumonia and from ordinary interstitial pneumonia]. ...
Asbestos bodies in bronchoalveolar lavage fluids of brake lining and asbestos cement workers. ... Asbestos bodies in bronchoalveolar lavage fluids of brake lining and asbestos cement workers. ... Examination of repeated bronchoalveolar lavage samples showed that the mechanisms of clearance of chrysotile fibres do not ... Asbestos body (AB) concentrations in bronchoalveolar lavage samples of 15 brake lining (BL) workers exposed only to chrysotile ...
Procoagulant (thromboplastin) activity in human bronchoalveolar lavage fluids is derived from alveolar macrophages. T Lyberg, B ... In the present study we found that both lung alveolar macrophages (LAM) and bronchoalveolar lavage fluids (BALF) from humans ... Procoagulant (thromboplastin) activity in human bronchoalveolar lavage fluids is derived from alveolar macrophages ... Procoagulant (thromboplastin) activity in human bronchoalveolar lavage fluids is derived from alveolar macrophages ...
... Thorax 41(4): 266-273. ... IgG and IgA concentrations were three times greater in lavage fluid from granite workers than the samples from non-industrial ... Immunoglobulin concentrations and lymphocyte counts were determined in bronchoalveolar fluid obtained from nine symptomless, ... in the lavage fluid whereas eight of nine (89%) granite workers had detectable IgM (chi 2 = 8, p less than 0.01). Lymphocyte ...
Haemosiderin-laden macrophages in the bronchoalveolar lavage fluid of patients with diffuse alveolar damage. *F. Maldonado 1 , ... Bronchoalveolar lavage fluid analysis. BAL was performed prior to surgical lung biopsy in all patients after a median interval ... Dot plot showing the percentage of haemosiderin-laden macrophages (HLMs) in bronchoalveolar lavage fluid from 21 patients with ... Subset analysis of patients with ≥20% haemosiderin-laden macrophages in bronchoalveolar lavage fluid. As already noted, seven ...
The extracellular vesicles derived from murine bronchoalveolar lavage fluid by both methods are quantified and characterized. ... Isolation of Extracellular Vesicles from Murine Bronchoalveolar Lavage Fluid Using an Ultrafiltration Centrifugation Technique ... Isolation of Extracellular Vesicles from Murine Bronchoalveolar Lavage Fluid Using an Ultrafiltration Centrifugation Technique ... to isolate extracellular vesicles from murine bronchoalveolar lavage fluid samples. ...
Cellular profiles of bronchoalveolar lavage fluid in 166 non-HIV-infected patients with Pneumocystis jirovecii pneumoniaa ... Cellular Profiles of Bronchoalveolar Lavage Fluid and Their Prognostic Significance for Non-HIV-Infected Patients with ... Cellular Profiles of Bronchoalveolar Lavage Fluid and Their Prognostic Significance for Non-HIV-Infected Patients with ... Cellular Profiles of Bronchoalveolar Lavage Fluid and Their Prognostic Significance for Non-HIV-Infected Patients with ...
Complement factor H is elevated in bronchoalveolar lavage fluid and sputum from patients with lung cancer. Ruben - Pio, Javier ... Complement factor H is elevated in bronchoalveolar lavage fluid and sputum from patients with lung cancer ... Complement factor H is elevated in bronchoalveolar lavage fluid and sputum from patients with lung cancer ... Complement factor H is elevated in bronchoalveolar lavage fluid and sputum from patients with lung cancer ...
Complement Factor H Is Elevated in Bronchoalveolar Lavage Fluid and Sputum from Patients with Lung Cancer. Ruben Pio, Javier ... Evaluation of factor H in bronchoalveolar lavage fluids. Quantification of complement factor H was first done in samples from ... The content of complement factor H was also assessed in an independent series of bronchoalveolar lavage fluids from unselected ... Sensitivity and specificity of cytologic examination and the factor H and albumin tests in the bronchoalveolar lavage fluids ...
... Respir Res. 2016 Jun 4;17(1):69. doi ... This study aimed to find proteins in bronchoalveolar lavage (BAL) fluid that can be associated with the disease. ... Methods: We developed and performed profiling of 94 selected proteins in BAL fluid and serum samples obtained from newly ... In addition, cadherin 5 (CDH5) was found to correlate positively with lymphocyte cell numbers in BAL fluid. ...
Lower concentrations of uncorrected glutathione in the bronchoalveolar lavage fluid were found on the first day of life in ... These preliminary results suggest that glutathione accumulates in the lung epithelial lining fluid of preterm infants and that ...
"Cryptococcal antigen detection in broncho-alveolar lavage fluid, Medical Mycology" on DeepDyve, the largest online rental ... Cryptococcal antigen detection in broncho-alveolar lavage fluid. Cryptococcal antigen detection in broncho-alveolar lavage ... broncho-alveolar lavage fluid; CSF, cerebrospinal fluid; FLC, fluconazole; 5FC, flucytosine; HIV, human immunodeficiency virus ... broncho-alveolar lavage fluid; CSF, cerebrospinal fluid; FLC, fluconazole; 5FC, flucytosine; HIV, human immunodeficiency virus ...
Chemotactic activity of bronchoalveolar lavage fluid from children with recurrent obstructive bronchitis ... Identification of C5a des arg and a cationic eosinophil chemotactic activity in the bronchoalveolar lavage fluid of asthmatics ... Chemotactic monocyte and activation factor (MCAF/MCP1)in material from bronchoalveolar lavage fluid of patients with chronic ... Chemotactic activity in bronchoalveolar lavage fluid from patients with adult respiratory distress syndrome. American Review of ...
Bronchoalveolar Lavage Fluid and Plasma Proteins, Chemiluminescence Response and Protein Contents of Polymorphonuclear ...
... proven by direct virus isolation with PCR from bronchoalveolar lavage fluid. CT findings and pulmonary function testing ... proven by direct virus isolation with PCR from bronchoalveolar lavage fluid. CT findings and pulmonary function testing ... virus isolation in bronchoalveolar lavage fluid. BMJ Case Reports:bcr2015210826. ...
  • In this study, effects of C. sinensis methanolic extracts on bronchoalveolar lavage fluids (BALF) cells proliferation, inflammatory cytokines production, and genes expression were evaluated. (nih.gov)
  • Analysis of fatty acids fractions of phospholipids and neutral lipids from bronchoalveolar lavage fluid (BALF) in patients with occupational lung disease (OLD). (cdc.gov)
  • We sought to determine if cellular and cytokine profiles in bronchoalveolar lavage fluid (BALF) from subjects with NEHI and FB would differ from pediatric disease controls. (nih.gov)
  • We measured the serum and BAL fluid (BALF) levels of IL-18 and IFN-gamma in 22 patients with IIP (12 with UIP, 10 with NSIP) and 9 healthy volunteers. (unboundmedicine.com)
  • In the present study we found that both lung alveolar macrophages (LAM) and bronchoalveolar lavage fluids (BALF) from humans contained procoagulant activities. (ersjournals.com)
  • Quantification of haemosiderin-laden macrophages in bronchoalveolar lavage fluid (BALF) has been used to diagnose diffuse alveolar haemorrhage (DAH) but has not been assessed in patients with diffuse alveolar damage (DAD). (ersjournals.com)
  • Perhaps the most commonly employed BAL criterion is the presence of ≥20% haemosiderin-laden macrophages in BAL fluid (BALF) 12 . (ersjournals.com)
  • In this study, we reviewed the performances of the CryAg detection (Premier EIA, Meridian) routinely performed in broncho-alveolar lavage fluid (BALF) during a 7-year period (2007-2013). (deepdyve.com)
  • Indeed, encapsulated yeasts may be missed due to the cellularity of the broncho-alveolar lavage fluid (BALF) or, by the contrary, some cells may be mistaken for yeasts leading to false-positive results. (deepdyve.com)
  • To further define the pulmonary inflammatory response, we conducted a proteome-based screen of bronchoalveolar lavage fluid (BALF) collected from young children with and without CF experiencing endobronchial infection. (edu.au)
  • Although studies have suggested that T cell subsets and cytokines in the Bronchoalveolar Lavage Fluid (BALF) may reflect the severity of pneumonia, the exact connection remains un-clarified. (alliedacademies.org)
  • Therefore, analysis of bronchoalveolar lavage fluid (BALF) to support stannosis might be of significant value. (pagepressjournals.org)
  • Bronchoalveolar lavage fluid (BALF) is a rich milieu of macrophages that form the first line of defense against invading TB bacilli. (currenthivresearch.com)
  • Cell analysis and chemokines in bronchoalveolar lavage fluid (BALF) were analyzed at three days, one week, one month, three months, and six months after the instillation. (mdpi.com)
  • To further investigate this potential pathogenic relationship, we used ELISA-based techniques to assess anti-citrullinated HSP90 antibody profiles in bronchoalveolar lavage fluid (BALF) of patients with different stages of RA-ILD. (elsevier.com)
  • Laboratory data from both patients showed extensive eosinophilia, their chest X-ray findings revealed multiple pulmonary infiltrates, and their bronchoalveolar lavage fluid (BALF) showed an elevated eosinophil count. (ajtmh.org)
  • BACKGROUND: Bronchoalveolar lavage fluid (BALF) is an important diagnostic source to investigate molecular changes occurring in lung disorders. (unica.it)
  • We evaluated neutrophil involvement in subclinical pulmonary emphysema by measuring human neutrophil lipocalin (HNL) and two matrix metalloproteinases, gelatinase B (MMP-9) and neutrophil collagenase (MMP-8), in bronchoalveolar lavage fluid (BALF) from 65 community-based older volunteers. (elsevier.com)
  • The bronchoalveolar lavage fluid (BALF) from LPS- or bacteria-injected mice stimulated migration of tumor cells. (aacrjournals.org)
  • The aim of our study was to characterize the inflammatory response associated with airway hyperresponsiveness, and to determine whether the inflammatory cell response of bronchoalveolar lavage fluid (BALF) reflected changes in the bronchioles in this model. (elsevier.com)
  • The puppies were killed 3-4 days after inoculation, the lungs excised, the intermediate lobe lavaged, and BALF and the bronchiolar wall tissue examined for neutrophils and other inflammatory cells. (elsevier.com)
  • Here, we present a case of desquamative interstitial pneumonia (DIP) that was initially misdiagnosed as chronic eosinophilic pneumonia due to an increased number of eosinophils in the bronchoalveolar lavage fluid (BALF). (e-sciencecentral.org)
  • The analysis of cellular components of the bronchoalveolar lavage fluid (BALF) informs us about inflammatory and immune processes in the alveolar space. (biomedcentral.com)
  • Objectives -To determine effects of selectin inhibitor TBC1269 on neutrophil infiltration, and neutrophilassociated injury during pneumonia induced by Mannheimia haemolytica and concentration of antimicrobial anionic peptide (AAP) in bronchoalveolar lavage fluid (BALF) as well as antimicrobial activity of BALF from healthy (control) neonatal calves, neonatal calves with M haemolytica -induced pneumonia, neonatal calves with prior treatment with TBC1269, and adult cattle. (iastate.edu)
  • Bronchoalveolar lavage fluid (BALF) has higher sensitivity than sputum for detection of Mycobacterium tuberculosis (Mtb). (biomedcentral.com)
  • Then, nested PCR was carried out to detect trichomonads in bronchoalveolar lavage fluid (BALF). (beds.ac.uk)
  • To determine the prevalence of Trichomonas infection in the lungs, bronchoalveolar lavage fluids (BALF) from 115 cases were tested using nested PCR and microscopy. (beds.ac.uk)
  • dpi, the virus induced significant increases in IL-17A in the bronchoalveolar lavage fluid (BALF) and serum. (pubmedcentralcanada.ca)
  • Induced sputum (IS) cellular profile was compared with bronchoalveolar lavage fluid (BALF) profile in asbestos exposed workers in order to assess its usefulness in monitoring workers exposed to asbestos for a long period of time. (elsevier.com)
  • Kokkinis, Fivos P. / Comparative analysis of induced sputum and bronchoalveolar lavage fluid (BALF) profile in asbestos exposed workers . (elsevier.com)
  • During August-October 2019, BAL fluid specimens were collected by clinical teams caring for hospitalized EVALI patients. (cdc.gov)
  • The quality of case-associated BAL specimens was assessed by measuring dipalmitoylphosphatidylcholine (DPPC), the principal phospholipid in naturally-occurring lung surfactant: the presence of acceptable levels of DPPC confirms that the lavage procedure recovered adequate pulmonary epithelial fluid. (cdc.gov)
  • Among the 27 BAL fluid specimens with sufficient volume for testing, all had measurable levels of DPPC. (cdc.gov)
  • In their recent article, Roberts and colleagues [1] reported cell counts and differentials in specimens obtained by bronchoalveolar lavage before and after radiotherapy in 17 women with breast cancer. (annals.org)
  • The authors did not, however, provide an explanation for the striking elevation in the mean percentage of lymphocytes in bronchoalveolar lavage specimens obtained before irradiation. (annals.org)
  • Bronchoscopic procedures for the diagnosis of lung cancer include cytologic examination of specimens from bronchial biopsy, bronchial brushing, and bronchial washing/bronchoalveolar lavage. (aacrjournals.org)
  • Samples from bronchoalveolar lavage fluid specimens returned the highest sensitivity. (wikipedia.org)
  • A PCR/ligase chain reaction technique was used to determine K-ras codon 12 mutations in a group of 52 bronchoalveolar lavage specimens from patients at risk of a second lung cancer. (elsevier.com)
  • We describe a novel method to measure petroleum distillates in BAL fluid using gas chromatography-mass spectrometry (GC/MS). Methods: n−Hexane, n−heptane, n−octane, methylcyclopentane, and cyclohexane were measured in BAL fluid specimens by headspace solid-phase microextraction /gas chromatography/mass spectrometry. (cdc.gov)
  • Comparison between real-time PCR, conventional PCR and different staining techniques for diagnosing Pneumocystis jiroveci pneumonia from bronchoalveolar lavage specimens. (microbiologyresearch.org)
  • We studied whether elevated α-amylase in mini bronchoalveolar lavage specimens can be suggestive of ventilator-associated pneumonia in intubated patients with high clinical suspicion. (blogspot.com)
  • The BAL fluid showed many erythrocytes, hemosiderin-laden macrophages, and sheets of endometrial stromal cells. (koreamed.org)
  • The expression of HLA-DR (Ia-like) antigens on human macrophages was investigated by analyses of cells from bronchoalveolar lavage fluid obtained from 12 patients with pulmonary sarcoidosis, six patients with extrinsic allergic alveolitis, nine patients with cryptogenic fibrosing alveolitis, 11 normal non-smokers, and 12 normal smokers. (uni-regensburg.de)
  • Previously a significant influx of exudate macrophages and persistence of bacteria on the bronchoalveolar surface were detected in these patients and a severe mucosal inflammation was observed bronchoscopically. (elsevier.com)
  • These tests can also be performed on cerebrospinal fluid (CSF) upon suspicion of coccidioidal meningitis. (medscape.com)
  • How to prepare bronchoalveolar lavage fluid, cerebrospinal fluid sample while using FineTest® Elisa kit? (fn-test.com)
  • The expression of these markers was also increased on lavage cells from patients with radiographic evidence of widespread disease (chest radiographic stage II and III), but there was no relation with disease duration, pulmonary function, or serum angiotensin converting enzyme activity. (bmj.com)
  • We developed and performed profiling of 94 selected proteins in BAL fluid and serum samples obtained from newly diagnosed and non-treated patients with sarcoidosis. (nih.gov)
  • Cryptococcal antigen detection in broncho-alveolar lavage fluid 2018-08-01 00:00:00 Abstract Cryptococcal antigen (CryAg) testing in serum and CSF is a clue diagnostic tool for cryptococcosis. (deepdyve.com)
  • Presumably, other chemo-attractants (leukotriens, enzymes) can also be found in the lavage fluid beside macrophage- or serum-derived and locally activated complement components. (eurekamag.com)
  • A comparison of complement components/albumin ratios in BAL fluid with serum complement components/albumin ratios suggested a significant local production and/or concentration of these factors. (eurekamag.com)
  • The relative lavage fluid levels of immunoglobulins to albumin were significantly higher than in serum, indicating a local production of these proteins. (elsevier.com)
  • Vitronectin was detected in lavage fluid and serum of all study subjects. (nebraska.edu)
  • Furthermore, on SDS-PAGE and Western blot analysis lavage fluid vitronectin comigrated with serum vitronectin, suggesting similar molecular size. (nebraska.edu)
  • Thus, vitronectin is a normal constituent of the epithelial lining fluid, and lavage fluid vitronectin is similar to serum vitronectin. (nebraska.edu)
  • Serum and bronchoalveolar lavage fluid levels of several tumor markers were measured in 71 patients with lung cancer, 36 patients with benign lung diseases and 100 healthy controls. (elsevier.com)
  • Fujii, M & Kiura, K 1988, ' Clinical evaluation of tumor markers in serum and bronchoalveolar lavage fluid in patients with lung cancer ', Japanese Journal of National Medical Services , vol. 42, no. 11, pp. 1012-1016. (elsevier.com)
  • The number of cells in bronchoalveolar lavage fluid from nano-TiO2 (5mg/kg)-exposed rats 0 (n = 10), 1 (n = 20), 2 (n = 15), 8 (n = 11), 16 (n = 5), 30 (n = 6), and 90 (n = 6) days post-intratracheal instillation. (nih.gov)
  • Brenchley, Jason M. / Reconstitution of CD4 T cells in bronchoalveolar lavage fluid after initiation of highly active antiretroviral therapy . (elsevier.com)
  • Pneumocystis carinii pneumonia: detection of parasites in sputum and bronchoalveolar lavage fluid by monoclonal antibodies. (microbiologyresearch.org)
  • Among idiopathic interstitial pneumonia (IIP), nonspecific interstitial pneumonia (NSIP) has an increased number of lymphocytes in bronchoalveolar lavage (BAL) fluid compared with usual interstitial pneumonia (UIP). (unboundmedicine.com)
  • Immunoglobulin levels in bronchoalveolar lavage fluid of children with recurrent obstructive bronchitis. (elsevier.com)
  • Bronchoalveolar lavage was performed in 22 children with recurrent obstructive bronchitis and the recovered lavage fluid samples were analysed for concentration of IgA, IgG, IgM, IgE and C3. (elsevier.com)
  • Bronchoscopy and bronchoalveolar lavage † (BAL) can be part of the clinical and diagnostic workup of EVALI patients. (cdc.gov)
  • 2 Bronchoscopy and bronchoalveolar lavage (BAL) are not routinely performed in such patients. (bmj.com)
  • To quantify relationships among collectins, bacteria, and inflammation in early cystic fibrosis (CF) airway secretions, bronchoalveolar lavage fluids (BALFs) were collected from children undergoing clinically indicated bronchoscopy. (mysciencework.com)
  • When using bronchoscopy for alveolar lavage, oral contact is avoided as much as possible. (beds.ac.uk)
  • In sarcoidosis, increased Th17 cell fractions have been reported in bronchoalveolar lavage fluid, and elevated numbers of Th17 cells producing IFN- γ have been observed in peripheral blood. (hindawi.com)
  • We also performed repeated TCR V gene analyses on some HLA-DR3+ patients and found an association between the ratio bronchoalveolar lavage fluid/peripheral blood V alpha 2.3+ CD4+ T cells and clinical signs of disease activity. (pnas.org)
  • Finally, when analyzing TCR V gene usage by CD8+ bronchoalveolar lavage fluid and peripheral blood T cells, a normal V alpha 2.3 usage was found in all cases, but lung-restricted T-cell expansions using other TCR V gene segment products were identified. (pnas.org)
  • Furthermore, 70% and 45% of bronchoalveolar lavage fluids from central and peripheral tumors, respectively, reported as cytologically negative were classified as positive using this marker. (aacrjournals.org)
  • Patients with asthma generate increased amounts of reactive oxygen species (ROS) from peripheral blood cells and cells recovered by bronchoalveolar lavage (BAL). (northwestern.edu)
  • In contrast, the depletion of the lower-respiratory-tract mucosal CD4 T cells as measured in bronchoalveolar lavage (BAL) fluid is more moderate and similar to the depletion of CD4 T cells observed in peripheral blood (PB). (elsevier.com)
  • In this study, we aimed to compare the human NK cell subset (CD56(bright or dim)) levels in the peripheral blood and bronchoalveolar lavage (BAL) fluid of sepsis patients. (unicamp.br)
  • Although we were able to confirm previous observations of peripheral blood lymphopenia, no significant differences were detected in NK cell levels in the BAL fluid of these patients. (unicamp.br)
  • The usefulness of bronchoalveolar lavage (BAL) fluid cellular analysis in non-human immunodeficiency virus (HIV)-infected patients with Pneumocystis jirovecii pneumonia (PCP) has not been adequately evaluated. (asm.org)
  • en] OBJECTIVE: To assess the predictive value of the endotoxin level in the bronchoalveolar lavage (BAL) and to propose to the clinician a guide in the diagnosis of gram-negative bacterial (GNB) pneumonia. (ac.be)
  • Levels of alpha-amylase in bronchoalveolar lavage (BAL) fluid can be used to detect pulmonary aspiration and predict bacterial pneumonia in critically ill, intubated adults, US researchers report. (medwirenews.com)
  • BAL fluid from 94 episodes of pneumonia in 81 patients was analyzed. (biomedcentral.com)
  • Acute eosinophilic pneumonia due to toxocariasis with bronchoalveolar lavage findings. (nih.gov)
  • In bronchoalveolar lavage fluid, specific metabolic profiles correlated with bacterial organisms known to play a role in the pathogenesis of pneumonia in HIV-infected individuals. (biomedcentral.com)
  • Human metapneumovirus in bronchoalveolar lavage fluid of critically ill patients with suspected pneumonia. (maastrichtuniversity.nl)
  • Ventilator-associated pneumonia was confirmed from mini bronchoalveolar lavage microbial culture of greater than or equal to 104 cfu/mL, and patients were divided into ventilator-associated pneumonia and no ventilator-associated pneumonia groups. (blogspot.com)
  • Conclusions: Patients with ventilator-associated pneumonia within 72 hours from intubation have significantly elevated α-amylase concentrations in mini bronchoalveolar lavage fluid. (blogspot.com)
  • The proteins were measured in unconcentrated lavage fluid by means of a solid phase, enzyme linked immunosorbent assay. (eurekamag.com)
  • Unexpectedly, levels of factor H in bronchoalveolar samples correlate with levels of albumin, suggesting that the cause of factor H accumulation in bronchial fluid is not its direct secretion from tumor cells but the exudation of plasma proteins to the bronchial lumen. (aacrjournals.org)
  • This study aimed to find proteins in bronchoalveolar lavage (BAL) fluid that can be associated with the disease. (nih.gov)
  • AbstractObjective To determine the concentration of proteins and phospholipids, markers of inflammatory reaction such as platelet-activating factor (PAF), and cell alterations in bronchoalveolar lavage (BAL) fluid during the evolution of the acute respiratory distress syndrome (ARDS). (semanticscholar.org)
  • Database of bronchoalveolar lavage fluid proteins. (ac.be)
  • Pulmonary Medicine, "Retracted: Predictive Role of ADA in Bronchoalveolar Lavage Fluid in Making the Diagnosis of Pulmonary Tuberculosis," Pulmonary Medicine , vol. 2014, Article ID 426964, 1 pages, 2014. (hindawi.com)
  • It is expected that new approaches, such as the detection of molecular markers, could be used in the future to enhance the sensitivity of cytologic examination in the diagnosis of bronchial fluids. (aacrjournals.org)
  • Detection of Aspergillus DNA in BAL fluid by quantitative PCR (qPCR) offers the potential for earlier diagnosis and higher sensitivity. (biomedcentral.com)
  • The Aspergillus qPCR assay detected Aspergillus DNA in 76.9% of subjects with proven or probable IPA when the concentrated BAL fluid pellet fraction was used for diagnosis. (biomedcentral.com)
  • A high degree of eosinophilia in the differential cell count of BAL fluid may eventually prove to be a useful clue in favor of such a diagnosis. (nih.gov)
  • F. Binesh and A. Halvani -Predictive role of ADA in bronchoalveolar lavage fluid in making the diagnosis of pulmonary tuberculosis,- Pulmonary Medicine, vol. 2013, Article ID 851518, 4 pages, 2013. (duhnnae.com)
  • A positive AspLFD test in BAL fluid of patients with classic risk factors for IPA could be useful to support the diagnosis of proven/probable IPA because of its high specificity . (bvsalud.org)
  • Results: There was a significantly higher concentration of factor H in bronchoalveolar lavage samples from lung cancer patients. (aacrjournals.org)
  • The use of human bronchoalveolar lavage fluid and induced sputum to conduct pulmonary proteomics. (ac.be)
  • Lemen, Richard J. / Bronchoalveolar lavage fluid cytology reflects airway inflammation in beagle puppies with acute bronchiolitis . (elsevier.com)
  • This differential is not sufficient to explain the findings of a mean bronchoalveolar lavage lymphocyte percentage of 34.5% in patients before irradiation. (annals.org)
  • We compared clinical characteristics and laboratory findings including bronchoalveolar lavage (BAL) fluid analysis in patients who experienced TMP/SMX treatment failure or ADR (the case group) versus those who did not (the control group). (bvsalud.org)
  • Methods: Complement factor H, a protein secreted by lung cancer cells, was quantified in a series of bronchoalveolar lavage supernatants from lung cancer patients and patients with non-malignant respiratory diseases. (aacrjournals.org)
  • METHODS: IL-9 protein was measured in bronchoalveolar lavage (BAL) fluid by means of ELISA and detected within the BAL cells by means of immunocytochemistry. (fraunhofer.de)
  • Furthermore, IL-9 protein and IL9 mRNA expression correlated with eosinophil numbers in BAL fluid. (fraunhofer.de)
  • Bronchoalveolar lavage fluid surfactant protein-A and surfacta. (mysciencework.com)
  • Bronchoalveolar lavage fluid surfactant protein-A and surfactant protein-D are inversely related to inflammation in early cystic fibrosis. (mysciencework.com)
  • Detection of Mycobacterium tuberculosis in sputum, pleural and bronchoalveolar lavage fluid using DNA amplification of the MPB 64 protein coding gene and IS6110 insertion element. (nus.edu.sg)
  • OBJECTIVES: To examine the effect of tumor necrosis factor-alpha (TNF-alpha) on pulmonary artery pressure and on total protein, phospholipid, lysophosphatidylcholine, phosphatidylcholine, phosphatidylinositol, and phosphatidylglycerol content in the bronchoalveolar lavage-accessible space of the isolated perfused rat lung, and to evaluate the role of the lung in the clearance of TNF-alpha from the perfusion medium in this model. (biomedsearch.com)
  • Bronchoalveolar lavage samples were subsequently analyzed for protein and phospholipid content. (biomedsearch.com)
  • Pathologic changes induced by endotoxin inhalation include bronchospasm, airflow obstruction, recruitment of inflammatory cells, injury of the alveolar epithelium, and disruption of pulmonary capillary integrity leading to protein-rich fluid leak in the alveolar space ( 6 , 7 ). (jimmunol.org)
  • PMN accumulation in the lung (myeloperoxidase assay), bronchoalveolar lavage (BAL) fluid, and lung vascular permeability (protein content in BAL fluid) were assessed 6 h later. (physiology.org)
  • a similar trend was seen in the alveolar fluid (36.5 ± 9.4 vs 23.3 ± 3.0 μM/mg protein). (northwestern.edu)
  • To better characterize exposure among EVALI patients, CDC developed and validated isotope dilution mass spectrometry methods to analyze specific toxicants of concern and active compounds in case-associated BAL fluid. (cdc.gov)
  • These CDC analytic methods can identify vitamin E acetate, MCT oil (medium chain triglycerides), plant oils (long chain triglycerides), petroleum distillates (including mineral oil), diluent terpenes, cannabinoids, and nicotine in BAL fluid. (cdc.gov)
  • Bronchoalveolar lavage fluid cells were obtained from 22 patients with sarcoidosis and from 10 normal control subjects and investigated by immunocytological methods. (bmj.com)
  • The extracellular vesicles derived from murine bronchoalveolar lavage fluid by both methods are quantified and characterized. (jove.com)
  • Novel Methods for the Analysis of Toxicants in Bronchoalveolar Lavage Fluid Samples from E-cigarette, or Vaping, Product Use-Associated Lung Injury (EVALI) Cases: Selected Petroleum Distillates. (cdc.gov)
  • Methods: Mini bronchoalveolar lavage samples were collected within 72 hours of endotracheal intubation. (blogspot.com)
  • Since DAH is clinically occult in some patients, bronchoalveolar lavage (BAL) has been used in diagnosing this condition, particularly in patients at excessive risk in surgical lung biopsy, e.g. thrombocytopenic or unstable patients. (ersjournals.com)
  • In this review, we provide an overview of biomarkers for pediatric BPD and PH that have been identified in clinical studies using various biological fluids. (frontiersin.org)
  • The lymphocytes in lavage fluid from patients with sarcoidosis were characterised by increased expression of activation markers, such as interleukin-2 receptors (anti-Tac+), HLA-DR (RFDR+), and "blast" forms (expressing above normal concentrations of CD7 antigen). (bmj.com)
  • Asbestos body (AB) concentrations in bronchoalveolar lavage samples of 15 brake lining (BL) workers exposed only to chrysotile have been determined and compared with those from 44 asbestos cement (AC) workers extensively exposed to amphiboles. (bmj.com)
  • Immunoglobulin concentrations and lymphocyte counts were determined in bronchoalveolar fluid obtained from nine symptomless, healthy, non-smoking granite workers (mean age 45.6, range 22-56 years) and nine normal, non-smoking, non industrial controls (mean age 22.8, range 21-32 years). (eurekamag.com)
  • IgG and IgA concentrations were three times greater in lavage fluid from granite workers than the samples from non-industrial controls (p less than 0.02). (eurekamag.com)
  • The normal albumin concentration suggests that differences in permeability do not account for all of the increased immunoglobulin concentrations found in granite workers' lavage fluid and that some immunoglobulin is locally synthesised. (eurekamag.com)
  • Lower concentrations of uncorrected glutathione in the bronchoalveolar lavage fluid were found on the first day of life in seven infants who subsequently developed chronic lung disease when compared with 27 infants who did not require supplemental oxygen at 36 weeks' postconceptional age. (bmj.com)
  • Furthermore, it significantly reduced inflammatory cell counts and concentrations of cytokines such as MCP-1, MIP-1α, and MIP-2 in bronchoalveolar lavage fluid. (jimmunol.org)
  • The increase of vitronectin concentrations in the epithelial lining fluid of patients with interstitial lung disease suggests that vitronectin may contribute to the pathogenesis of interstitial lung disease. (nebraska.edu)
  • Mini bronchoalveolar lavage α-amylase concentrations increase with increasing number of aspiration risk factors. (blogspot.com)
  • Culture and histopathological examination of bronchoalveolar lavage (BAL) fluid are useful but have suboptimal sensitivity and in the case of culture may require several days for fungal growth to be evident. (biomedcentral.com)
  • Boyden's method was used to assess the chemotactic activity of bronchoalveolar lavage (BAL) fluid samples from patients aged 1-6 yrs with recurrent obstructive bronchitis when signs of chronic mucosal inflammation were observed bronchoscopically. (eurekamag.com)
  • Faculty Opinions recommendation of De novo assembly of the Pneumocystis jirovecii genome from a single bronchoalveolar lavage fluid specimen from a patient. (growkudos.com)
  • This finding suggests that the quantitative measurement of soluble CD138 in BAL fluid may reflect plasma cell infiltration and disease activity in the lungs of patients with MCD. (elsevier.com)
  • Tumor necrosis factor-alpha alters phospholipid content in the bronchoalveolar lavage-accessible space of isolated perfused rat lungs. (biomedsearch.com)
  • After a baseline sampling bronchoalveolar lavage, a reduction bronchoalveolar lavage was performed to establish a uniform amount of phospholipid in all lungs. (biomedsearch.com)
  • Subsequently, bleomycin-induced up-regulation of TGF-β in bronchoalveolar lavage fluid was significantly inhibited, and collagen deposition in the lungs was reduced. (jimmunol.org)
  • MWCNT exposure markedly increased A 2B adenosine receptor (A 2B AR) expression in the lungs and ADO level in bronchoalveolar lavage fluid, combined with elevation of blood neutrophils, collagen fiber deposition, and activation of myeloperoxidase (MPO) activity in the lungs. (springer.com)
  • Our results show that bronchoalveolar lavage fluids from subjects with lung cancer present a significantly higher expression of factor H than those from patients with nonmalignant pulmonary diseases. (aacrjournals.org)
  • In comparison with the chemotactic effect of casein, the activity of the fluid proved to be high for polymorphonuclear leukocytes (PMNs), but significantly low for mononuclear cells. (eurekamag.com)
  • Further metabolite analysis demonstrated that four features were significantly overrepresented in the bronchoalveolar lavage (BAL) fluid of HIV-infected individuals compared to HIV-uninfected, including cystine, two complex carbohydrates, and 3,5-dibromo- l -tyrosine. (biomedcentral.com)
  • In addition, cadherin 5 (CDH5) was found to correlate positively with lymphocyte cell numbers in BAL fluid. (nih.gov)
  • MEASUREMENTS AND MAIN RESULTS: Quantitative cultures for aerobic bacteria were performed directly from the fluid. (ac.be)
  • Detection of toxicants in bronchoalveolar-Iavage (BAL) fluid from patients with EVALI can provide direct information on exposure within the lung. (cdc.gov)
  • With the use of sensitive detection techniques, we report a higher prevalence of K-ras mutations in bronchoalveolar lavage than has been reported previously for lung cancer. (elsevier.com)
  • The aim of this study was to evaluate the inter-laboratory agreement of three independently developed real-time PCR assays for the detection of P. jiroveci in bronchoalveolar lavage fluid samples. (microbiologyresearch.org)
  • It is concluded that occupational exposure to granite dust is associated with an increased proportion of lymphocytes and an increased concentration of immunoglobulin in lavage fluid that may reflect a subclinical immune inflammatory response. (eurekamag.com)
  • Using three different liquid chromatography coupled to tandem mass spectrometry targeted platforms (LC-MS/MS), we quantified a broad suite of lipid mediators including eicosanoids, sphingolipids and endocannabinoids in bronchoalveolar lavage (BAL) fluid from pulmonary sarcoidosis patients ( n = 41) and healthy controls ( n = 16). (springer.com)
  • citation needed] Lung washings or tissue for histopathologic analysis are most commonly obtained using bronchoalveolar lavage and/or lung biopsy. (wikipedia.org)
  • In addition, novel histopathological, biochemical, and molecular factors have been identified in the lung tissue and in biological fluids of BPD and PH patients that could associate with the disease phenotype. (frontiersin.org)
  • Systemic LPS administration induces neutrophil sequestration into the pulmonary microvasculature without passage into the lung tissues and bronchoalveolar space ( 12 ), whereas neutrophil recruitment upon aerogenic LPS exposure occurs in all airway compartments ( 8 ). (jimmunol.org)
  • 02). CONCLUSIONS: a) TNF-alpha alters the amounts of phosphatidylcholine, phosphatidylinositol, and possibly phosphatidylglycerol present in the lavage-accessible space of the isolated perfused rat lung. (biomedsearch.com)
  • In fact, some molecular markers have already been shown to be differentially increased in bronchial fluids from lung cancer patients. (aacrjournals.org)
  • The aim of the present study is to explore whether factor H levels are detectable in bronchial fluids of lung cancer patients and to evaluate its potential diagnostic usefulness. (aacrjournals.org)
  • Bronchoalveolar lavage vs. bronchial brushing. (microbiologyresearch.org)
  • The fluids recovered from the first 20-ml aliquot and that from the next four aliquots were labeled bronchial and alveolar fluid, respectively. (northwestern.edu)
  • Adults were in need of mechanical ventilation, and testing of BAL fluid was performed within 72 hours of endotracheal intubation. (medwirenews.com)
  • Examination of repeated bronchoalveolar lavage samples showed that the mechanisms of clearance of chrysotile fibres do not affect AB concentration for at least 10 months after cessation of exposure. (bmj.com)
  • Immunocytological analysis of lavage cells offers a probe for studying the pathogenesis of sarcoidosis and may be of value in monitoring disease activity. (bmj.com)
  • The receiver operating characteristic curve analysis showed that the Limulus assay still had a good discrimination power in the prediction of significant bacterial count in BAL fluid. (ac.be)
  • Proteome analysis of bronchoalveolar lavage fluids reveals hos. (mysciencework.com)
  • Analysis of bronchoalveolar lavage (BAL) fluid showed 64 percent eosinophils. (nih.gov)
  • In this study, the levels of SP-D in sera and bronchoalveolar lavage (BAL) fluids of patients with lung diseases were determined by ELISA, using human recombinant SP-D as a standard. (elsevier.com)
  • The mean levels of bronchoalveolar lavage fluid CEA, ferritin and β 2 MG in patients with lung cancer were statistically higher than those in patients with benign lung disease and healthy controls. (elsevier.com)
  • Bronchoalveolar lavage (BAL) fluid samples from 51 EVALI and 99 non-EVALI cases were analyzed for toxicants including petroleum distillates. (cdc.gov)
  • A mini bronchoalveolar lavage α-amylase of 163 U/L or more yielded sensitivity and specificity of 73% and 68.6%, respectively, with area under the receiver operating characteristic curve of 0.746 (95% CI, 0.66-0.83). (blogspot.com)
  • This is the first report to demonstrate a marked elevation of soluble CD138, despite the absence of plasma cells, in BAL fluid in an MCD patient with pulmonary involvement. (elsevier.com)
  • Retired underground miners were investigated to document alveolitis, and to observe the difference in the cellular profiles of bronchoalveolar lavage (BAL) fluid with or without pneumoconiosis. (bmj.com)
  • Alveolitis was not a representative feature of Turkish subjects with an occupational history of underground mining, and BAL fluid cellular profile did not seem to be different in miners with or without pneumoconiosis. (bmj.com)
  • We conducted a clinical trial to document alveolitis in miners, if any, and to observe the differences in the cellular profiles of BAL fluid of miners exposed to inorganic dust but who had not developed pneumoconiosis and those with different categories of pneumoconiosis. (bmj.com)
  • Clinical application of Aspergillus lateral flow device in bronchoalveolar lavage fluid of patients with classic risk factors for invasive pulmonary aspergillosis. (bvsalud.org)
  • Regulation of bronchoalveolar lavage fluids cell function by the immunomodulatory agents from Cordyceps sinensis. (nih.gov)
  • This study was designed to determine whether cell populations in bronchoalveolar lavage fluid represent a reflection of disease activity in sarcoidosis. (bmj.com)
  • To understand better the dynamics of disease pathogenesis and the potential for the reconstitution of CD4 T cells in the lung and PB following the administration of effective antiretroviral therapy, we studied cell-associated viral loads, CD4 T-cell frequencies, and phenotypic and functional profiles of antigen-specific CD4 T cells from BAL fluid and blood before and after the initiation of highly active antiretroviral therapy (HAART). (elsevier.com)
  • An investigation was conducted of the lipidic composition (phospholipids, neutral lipids and fatty acids) of the extracellular compartment of the pulmonary surfactant, obtained through bronchoalveolar lavage in 15 individuals: five normal, five pigeon breeders, and five silicosis patients. (cdc.gov)
  • Here, we describe two extracellular vesicle isolation protocols, ultrafiltration centrifugation and ultracentrifugation with density gradient centrifugation, to isolate extracellular vesicles from murine bronchoalveolar lavage fluid samples. (jove.com)
  • Bronchoalveolar lavage (BAL) fluid is routinely used to assess for the presence of fungi at the site of pulmonary infection. (biomedcentral.com)