A dye that has been used as an industrial dye, a laboratory indicator, and a biological stain.

Procedure for calibrating the Technicon Colorimeter I. (1/29)

We describe a rapid method for calibrating the Technicon AutoAnalyzer colorimeter I. Test solutions of bromphenol blue are recommended for the calibration, in preference to solutions of potassium dichromate, based on considerations of the instrument's working range and of the stray light characteristics of the associated filters.  (+info)

Guidance for selecting the measurement conditions in the dye-binding method for determining serum protein: theoretical analysis based on the chemical equilibrium of protein error. (2/29)

A methodology for selecting the measurement conditions in the dye-binding method for determining serum protein has been studied by a theoretical calculation. This calculation was based on the fact that a protein error occurs because of a reaction between the side chains of a positively charged amino acid residue in a protein molecule and a dissociated dye anion. The calculated characteristics of this method are summarized as follows: (1) Although the reaction between the dye and the protein occurs up to about pH 12, a change in the color shade, called protein error, is observed only in a pH region restricted within narrow limits. (2) Although the apparent absorbance (the absorbance of the test solution measured against a reagent blank) is lower than the true absorbance indicated by the formed dye-protein complex, the apparent absorbance correlates with the true absorbance with a correlation coefficient of 1.0. (3) At a higher dye concentration, the calibration curve is more linear at a higher pH than at a lower pH. Most of these characteristics were similarly observed experimentally in the reactions of BPB, BCG and BCP with human and bovine albumins. It is concluded that in order to ensure the linearity of the calibration curve, the measurement should be performed at a higher dye concentration and sufficiently high pH where the detection sensitivity is satisfied.  (+info)

Microfluidic large-scale integration. (3/29)

We developed high-density microfluidic chips that contain plumbing networks with thousands of micromechanical valves and hundreds of individually addressable chambers. These fluidic devices are analogous to electronic integrated circuits fabricated using large-scale integration. A key component of these networks is the fluidic multiplexor, which is a combinatorial array of binary valve patterns that exponentially increases the processing power of a network by allowing complex fluid manipulations with a minimal number of inputs. We used these integrated microfluidic networks to construct the microfluidic analog of a comparator array and a microfluidic memory storage device whose behavior resembles random-access memory.  (+info)

Preparation of sand fly (Diptera: Psychodidae: Phlebotominae) specimens for histological studies. (4/29)

Phlebotominae sand fly specimens were prepared for histological and physiological studies. Different fixatives were tested on sectioned and whole bodied adult females in order to obtain good fixation and provide satisfactory penetration of the embedding media. All fixed specimens were infiltrated (up to seven days under 5 C) and embedded in hydroxyethyl metacrylate. Two-three m sections were stained, mounted in Canada balsam and observed by light microscopy. Best results were achieved when whole bodied insects were double fixed in Bouin's and Carnoy's fluids (4 h/2 h) and stained in Hematoxilin/Eosin or fixed in calcium formaldehyde and stained in mercury bromophenol blue.  (+info)

Purification and characterization of an aflatoxin degradation enzyme from Pleurotus ostreatus. (5/29)

Nineteen fungi were tested for their ability to degrade aflatoxin B1 (AFB1). An extracellular enzyme from the edible mushroom Pleurotus ostreatus showed afaltoxin-degradation activity detected by thin-layer chromatography (TLC). An enzyme with this activity was purified by two chromatographies on DEAE-Sepharose and Phenyl-Sepharose. The apparent molecular mass of the purified enzyme was estimated to be 90 kDa by SDS-PAGE. Optimum activities were found in the pH range between 4.0 and 5.0 and at 25 degrees C. Also, degradation activity of several dyes in the presence of H2O2 was tested, resulting in the detection of bromophenol blue-decolorizing activity. Based on these data, we suggest this enzyme is a novel enzyme with aflatoxin-degradation activity. Fluorescence measurements suggest that the enzyme cleaves the lactone ring of aflatoxin.  (+info)

Delivery of several forms of DNA, DNA-RNA hybrids, and dyes across human sclera by electrical fields. (6/29)

PURPOSE: Iontophoresis has been used for drug delivery across the cornea for many years. We sought to test whether small charged dyes and DNA can be transferred across human sclera by an electric field. METHODS: Full-thickness human scleral fragments were embedded vertically in an agarose gel and positioned to completely span individual gel lanes. The scleral fragments were located approximately 1 cm downstream from the gel wells. DNA or dyes were loaded into the wells and electrophoresis was carried out at about 3.3 V/cm for approximately 2 h per run. Movement of DNA and dyes through the agarose and sclera was measured with either digital time-lapse photography or through DNA extraction and purification from the gel. SYBR green stain was used as a sensitive method to detect DNA. RESULTS: Digital time-lapse photography of agarose gel electrophoresis revealed that two dyes, xylene cyanol and bromphenol blue, passed through the sclera in the presence of an electric field. Xylene cyanol was driven through the sclera virtually unimpeded except for some spreading of the dye. Bromphenol blue was slowed markedly by the sclera, but it too eventually passed through the tissue. Small DNAs, including a single stranded 51-mer and a double hairpin 68-mer oligonucleotide, passed through the sclera as detected by SYBR green staining. Linear double stranded DNAs ranging from 50 bp to 12,000 bp passed through the sclera. The larger the DNA, the slower the rate of passage through the sclera, and the greater the band spreading. pEGFP-1 (a 3 kb plasmid) passed through the sclera but was accompanied by a great amount of band spreading. Following completion of the initial electrophoresis run, the plasmid DNA was extracted from the smeared bands in the agarose distal to the sclera and re-run on a second gel without sclera. The initially smeared plasmid bands resolved into 2 distinct bands after extraction and purification and matched well with control plasmid bands. CONCLUSIONS: Charged molecules such as xylene cyanol, bromphenol blue, and DNAs ranging from 51 bp oligonucleotides to a 3 kb plasmid can be driven across human sclera by an electric field and directly detected. Passage of plasmids was efficient, but the plasmid bands were diffuse after transit. This technique offers promise as a noninvasive DNA delivery tool, where gene therapy can be accomplished by small RNA or DNA synthetic oligonucleotides, larger double stranded fragments, or even plasmids.  (+info)

Theoretical analysis concerning the characteristics of a dye-binding method for determining serum protein based on protein error of pH indicator: effect of buffer concentration of the color reagent on the color development. (7/29)

In the dye-binding method based on protein error of a pH indicator, the color development has been reported to be markedly affected by the buffer concentration of the color reagent. In this study, the author analyzed this phenomenon by a theoretical calculation based on the chemical equilibrium of protein error. The calculation was performed on the assumption that both the dissociated dye anion and the anion contained in the buffer solution react with protein, forming a dye-protein complex and an anion-protein complex, respectively. The calculated results were compared with those obtained by the experiments using bromophenol blue, bromocresol green and bromocresol purple that are employed widely for determining the human serum albumin concentration clinically. The calculated results of this method are summarized as follows: (1) the color development decreases with the increase in the concentration of the anion contained in the buffer solution; (2) the calibration curve is more linear in the higher concentration of the anion than in the lower one. These calculated results agreed well with the experimental ones. From these results, it was concluded that the change in the color development by the buffer concentration of the color reagent is due to the change in the concentration of the buffer anion.  (+info)

An evaluation of novel vital dyes for intraocular surgery. (8/29)

PURPOSE: To evaluate systematically the staining characteristics and safety of potential new dyes for intraocular surgery. METHODS: Six dyes were included in the investigation: light green SF (LGSF) yellowish, E68, bromophenol blue (BPB), Chicago blue (CB), rhodamine 6G, rhodulinblau-basic 3 (RDB-B3). All dyes were dissolved and diluted in a balanced saline saline solution. The light-absorbing properties of each dye were measured at a concentration of 0.05% between 200 and 1000 nm. Staining characteristics were examined by staining lens capsule tissue and epiretinal membranes (ERMs), removed intraoperatively, with dye concentrations of 1.0%, 0.5%, 0.2%, and 0.05%. Enucleated porcine eyes (postmortem time, 9 hours) were also stained. Dye-related toxicity was evaluated by a colorimetric test (MTT) measuring the inhibition of retinal pigment epithelium (RPE) cell proliferation (ARPE-19 and primary human RPE cells, passages 3-6). Cell viability was also quantified based on a two-color fluorescence cell-viability assay. Dyes were investigated in concentrations of 0.2% and 0.02%. RESULTS: All dyes investigated in this study stained human lens capsules, removed intraoperatively; ERMs, peeled during macular pucker surgery; and enucleated porcine eyes, depending on the concentration applied. The long-wavelength absorption maximum of the dyes was within the range of 527 to 655 nm at concentrations of 0.05%. Rhodamine G6 and RDB-B3 showed adverse effects on ARPE-19 cell proliferation at a concentration of 0.2% and were excluded from further investigation in primary RPE cells. The remaining four dyes showed no toxic effect on ARPE-19 and primary RPE cell proliferation at concentrations of 0.2% and 0.02%. Cell viability was affected by LGSF yellowish (0.2%) and CB (0.2% and 0.02%). Two dyes (E68 and BPB) showed no relevant toxicity in vitro. CONCLUSIONS: The systematic evaluation of dyes for intraocular use seems mandatory. In this study four dyes were identified with effective staining characteristics, with two of these dyes having no detectable toxic effect on RPE cells in vitro.  (+info)

Bromophenol Blue is a chemical compound that is commonly used as an indicator in acid-base titrations in chemistry and biology. Its chemical formula is C19H10Br4O5S. It is a dark green crystalline powder that is soluble in water and alcohol, and it has a molecular weight of 669.93 g/mol.

In solution, Bromophenol Blue exhibits different colors depending on the pH level. At pH levels below 3.0, it appears yellow; between 3.0 and 4.6, it is green; between 4.6 and 6.8, it is blue; and above 6.8, it turns purple. This color change makes it a useful tool for indicating the endpoint in acid-base titrations.

In addition to its use as an indicator, Bromophenol Blue has also been used in research and medical applications, such as staining proteins in gels and as a marker for protein denaturation. However, it should be handled with care, as it can cause irritation to the skin, eyes, and respiratory system, and is considered a hazardous substance.

"Bromphenol Blue - Compound Summary". PubChem Compound. Retrieved 2022-03-17. "Bromphenol Blue - Use and Manufacturing". PubChem ... Bromophenol blue is also used as a dye. At neutral pH, the dye absorbs red light most strongly and transmits blue light. (Its ... It changes from yellow at pH 3.0 to blue at pH 4.6; this reaction is reversible. Bromophenol blue is structurally related to ... Since bromophenol blue carries a slight negative charge at moderate pH, it will migrate in the same direction as DNA or protein ...
Mazia D, Brewer PA, Alfert MA (1953). "The Cytochemical Staining and Measurement of Protein with Mercuric Bromphenol Blue". The ...
... bromphenol blue MeSH D02.455.426.559.389.832.200 - bromthymol blue MeSH D02.455.426.559.389.832.500 - toluene 2,4-diisocyanate ... bromphenol blue MeSH D02.886.590.887.200 - bromthymol blue MeSH D02.886.590.887.570 - tosylarginine methyl ester MeSH D02.886. ... Evans blue MeSH D02.886.645.600.080.050.650.750 - suramin MeSH D02.886.645.600.080.050.650.875 - trypan blue MeSH D02.886. ... methylene blue MeSH D02.886.369.533 - moricizine MeSH D02.886.369.550 - nonachlazine MeSH D02.886.369.575 - perazine MeSH ...
Bromphenol Blue quantity. Add To Cart. Add to Compare. Add to Wishlist. SKU: SDS56 Category: Material Safety Data Sheets Tag: ...
"Bromphenol Blue - Compound Summary". PubChem Compound. Retrieved 2022-03-17. "Bromphenol Blue - Use and Manufacturing". PubChem ... Bromophenol blue is also used as a dye. At neutral pH, the dye absorbs red light most strongly and transmits blue light. (Its ... It changes from yellow at pH 3.0 to blue at pH 4.6; this reaction is reversible. Bromophenol blue is structurally related to ... Since bromophenol blue carries a slight negative charge at moderate pH, it will migrate in the same direction as DNA or protein ...
Bromphenol blue. 6.2-7.6. 1 drop 0.1% aq. soln.. yellow. blue. p-Nitrophenol. 5.0-7.0. 1-5 drops 0.1% aq. soln.. colorless. ... Bromphenol blue. 3.0-4.6. 1 drop 0.1% aq. soln.. yellow. blue-violet. ... Tetrabromphenol blue. 3.0-4.6. 1 drop 0.1% aq. soln.. yellow. blue. Alizarin sodium sulfonate. 3.7-5.2. 1 drop 0.1% aq. soln.. ... Poirriers blue. 11.0-13.0. 1 drop 0.1% aq. soln.. blue. violet-pink. ...
Fifteen μL of PCR product mixed with 3 μL 6X loading buffer (0.25% [wt/vol] bromphenol blue and 40% [wt/vol] sucrose) were ...
100 µg in 1 ml (100rxns) loading buffer (0.25% bromphenol blue, 0.25% xylene cyanol FF, 40% sucrose in water). Load 10 µ l in ...
... bromphenol blue). Samples were incubated for approximately one hour at room temperature. After pelleting the cell debris, ... Out of those, three transformants (apr1, apr4 and apr6), which did not turn blue, and one transformant (apr7), which needed ... Each well was titrated with Na2S2O3 (0.01 M) in 10 μl to 50 μl titration steps until the dark-blue color completely disappeared ... CC-2803, which produces less oxygen than it consumes, did not turn blue after the Winkler test, whereas the wild type needed to ...
D2.455.426.559.389.127.150 Bromphenol Blue D2.755.150 D2.455.426.559.389.657.150 Bumetanide D2.241.223.100.215 D2.241.223.100. ...
D2.455.426.559.389.127.150 Bromphenol Blue D2.755.150 D2.455.426.559.389.657.150 Bumetanide D2.241.223.100.215 D2.241.223.100. ...
Bromphenol Blue [D02.455.426.559.389.657.150] * Catechols [D02.455.426.559.389.657.166] * Chlorophenols [D02.455.426.559. ...
Bromphenol Blue. *Catechols. *Chlorophenols. *Cresols. *Dienestrol. *Humic Substances. *Hydroquinones. *Hydroxybenzoates. * ...
Damien Hirst "Bromphenol Blue". Out of stock Out of stock! Selling yours? Damien Hirst "To a stranger" ...
v) glycerol and 0.004 bromphenol blue, and boiled for 3 min. Lastly, the , ...
DETERMINATION OF MEXILETINE BY ION-PAIR EXTRACTION WITH BROMPHENOL BLUE #CEM ÖNAL,#ARMAĞAN ÖNAL,#SEDAT TOSUNOĞLU Turkish ... PAIR EXTRACTION USING BROMOPHENOL BLUE AND BROMOCRESOL PURPLE Ş EVRİM KEPEKÇİ, AYSEL ÖZTUNÇ Turkish Abstract Abstract Full Text ... BLUE FLOWER ROSEMARY (ROSMARINUS OFFICINALIS L.) FROM SUBTROPICAL INDIA RAM SWAROOP VERMA, KONENİ VENKATA SASHİDHARA, ANJU ...
DETERMINATION OF MEXILETINE BY ION-PAIR EXTRACTION WITH BROMPHENOL BLUE #CEM ÖNAL,#ARMAĞAN ÖNAL,#SEDAT TOSUNOĞLU Turkish ... PAIR EXTRACTION USING BROMOPHENOL BLUE AND BROMOCRESOL PURPLE Ş EVRİM KEPEKÇİ, AYSEL ÖZTUNÇ Turkish Abstract Abstract Full Text ... BLUE FLOWER ROSEMARY (ROSMARINUS OFFICINALIS L.) FROM SUBTROPICAL INDIA RAM SWAROOP VERMA, KONENİ VENKATA SASHİDHARA, ANJU ...
Bromphenol blue, sodium salt. Phenol red, sodium salt. Thymol blue, sodium salt. Ethyl alcohol, denatured. Water ...
The method is based on the interaction of the drug base with bromophenol blue to give a stable ion-pair complex. The spectra of ... Bromphenol Blue* Actions. * Search in PubMed * Search in MeSH * Add to Search ... Studies of complex formation between the bromophenol blue and some important aminoquinoline antimalarials S M el-Ashry 1 , F A ... Studies of complex formation between the bromophenol blue and some important aminoquinoline antimalarials S M el-Ashry et al. ...
... bromphenol blue, indigo carmine or bromcresol purple. Further additional data showed that the chromogenic albumin indicator BCG ...
... bromphenol blue and NuPage sample reducing agent [Invitrogen, Carlsbad, CA]) for 5 minutes to elute the FLAG-tagged protein. ...
For gel electrophoresis, 4 µL PCR product was mixed with one drop loading dye (0.3 mL 30% glycerol and 2.5 mg bromphenol blue/ ...
D2.455.426.559.389.127.150 Bromphenol Blue D2.755.150 D2.455.426.559.389.657.150 Bumetanide D2.241.223.100.215 D2.241.223.100. ...
Bromphenol Blue Preferred Concept UI. M0002958. Registry Number. 0R2969YC90. Related Numbers. 115-39-9. Scope Note. A dye that ... Bromphenol Blue Preferred Term Term UI T005641. Date01/01/1999. LexicalTag NON. ThesaurusID ... Bromphenol Blue. Tree Number(s). D02.455.426.559.389.657.150. D02.455.426.559.389.832.180. D02.886.590.887.180. Unique ID. ... Bromophenol Blue Tetrabromophenol Blue Pharm Action. Coloring Agents. Indicators and Reagents. Registry Number. 0R2969YC90. ...
Bromphenol Blue Preferred Concept UI. M0002958. Registry Number. 0R2969YC90. Related Numbers. 115-39-9. Scope Note. A dye that ... Bromphenol Blue Preferred Term Term UI T005641. Date01/01/1999. LexicalTag NON. ThesaurusID ... Bromphenol Blue. Tree Number(s). D02.455.426.559.389.657.150. D02.455.426.559.389.832.180. D02.886.590.887.180. Unique ID. ... Bromophenol Blue Tetrabromophenol Blue Pharm Action. Coloring Agents. Indicators and Reagents. Registry Number. 0R2969YC90. ...
Diphenylcarbazone-Bromphenol Blue, APHA, ASTM, EPA, D1815-100. Code:D1815-100. Price: $14.67. Quantity in Basket: none. ... Diphenylcarbazone-Bromphenol Blue, APHA, ASTM, EPA, D1815-500. Code:D1815-500. Price: $36.86. Quantity in Basket: none. ... DIPHENYLCARB-BROMPHENOL BLUE. Code:2610-4. Price: $27.61. Quantity in Basket: none. ... DIPHENYLCARB-BROMPHENOL BLUE. Code:2610-32. Price: $86.53. Quantity in Basket: none. ...
... bromphenol blue was added. The digests were then subjected to electrophoresis for 16-20 hr at a con- stant voltage of 160 V in ...
N0000006177 Brompheniramine N0000179532 Brompheniramine Maleate N0000166616 Bromphenol Blue N0000166474 Bromthymol Blue ... 6 N0000179130 FDC blue lake no.2 N0000179385 febuxostat N0000007445 felbamate N0000006368 Felodipine N0000170339 Felypressin ... Isatin N0000170915 ISCOMs N0000166482 Isethionic Acid N0000182046 Islet Amyloid Polypeptide N0000179160 iso-sulfan blue ... N0000169755 TRPP Cation Channels N0000169757 TRPV Cation Channels N0000178799 Truncated Hemoglobins N0000167386 Trypan Blue ...
M kilometers associated with bromphenol glowing blue looked implanted on the parenchyma involving a new clamped liver by having ...
Fifteen μL of PCR product mixed with 3 μL 6X loading buffer (0.25% [wt/vol] bromphenol blue and 40% [wt/vol] sucrose) were ...
Bromphenol blue (Sigma St Louis MO) and subjected to SDS-PAGE as described below. The membrane was immunoblotted with anti- ...
DYES BROMPHENOL BLUE DYES BROMTHYMOL BLUE DYES CARBOCYANINES DYES CARMINE DYES CHROMOMYCIN A3 DYES CLOFAZIMINE DYES CONGO RED ... INDICATORS AND REAGENTS BROMPHENOL BLUE INDICATORS AND REAGENTS BROMTHYMOL BLUE INDICATORS AND REAGENTS CHLOROMERCURIBENZOATES ... DYES ALCIAN BLUE DYES AMARANTH DYE DYES AMIDO BLACK DYES AMINACRINE DYES ARSENAZO III DYES AZURE STAINS DYES BENZOPHENONEIDUM ... DYES EVANS BLUE DYES FLUORESCEIN-5-ISOTHIOCYANATE DYES FLUORESCEINS DYES FLUORESCENT DYES DYES FURA-2 DYES HEMATOXYLIN DYES ...
D2.455.426.559.389.127.150 Bromphenol Blue D2.755.150 D2.455.426.559.389.657.150 Bumetanide D2.241.223.100.215 D2.241.223.100. ...
DETERMINATION OF MEXILETINE BY ION-PAIR EXTRACTION WITH BROMPHENOL BLUE #CEM ÖNAL,#ARMAĞAN ÖNAL,#SEDAT TOSUNOĞLU Turkish ... PAIR EXTRACTION USING BROMOPHENOL BLUE AND BROMOCRESOL PURPLE Ş EVRİM KEPEKÇİ, AYSEL ÖZTUNÇ Turkish Abstract Abstract Full Text ... BLUE FLOWER ROSEMARY (ROSMARINUS OFFICINALIS L.) FROM SUBTROPICAL INDIA RAM SWAROOP VERMA, KONENİ VENKATA SASHİDHARA, ANJU ...
DETERMINATION OF MEXILETINE BY ION-PAIR EXTRACTION WITH BROMPHENOL BLUE #CEM ÖNAL,#ARMAĞAN ÖNAL,#SEDAT TOSUNOĞLU Turkish ... PAIR EXTRACTION USING BROMOPHENOL BLUE AND BROMOCRESOL PURPLE Ş EVRİM KEPEKÇİ, AYSEL ÖZTUNÇ Turkish Abstract Abstract Full Text ... BLUE FLOWER ROSEMARY (ROSMARINUS OFFICINALIS L.) FROM SUBTROPICAL INDIA RAM SWAROOP VERMA, KONENİ VENKATA SASHİDHARA, ANJU ...
Green, GPR115; blue, DAPI. am, ameloblast; si, stratum intermedium; od, odontoblast; pa, papillary layer. Dashed lines indicate ... Left column, bromphenol red and resazurin staining of WT and Gpr115-KO incisors. Right column, quantified data showing stained ... Red and blue plots, up- and down-regulated genes, respectively. C, heat map of ion transporters expressed in P7 WT and Gpr115- ... Blue, molar enamel; yellow, incisor enamel. Arrows indicate position used for measurement of enamel mineral density in C. B, ...
... generate a specific blue colour. Its intensity, measured at 604 nm, is directly proportional to the concentration of soaps in ... At specific pH values soaps mixed with bromphenol, ...
Opaque, Dark Blue To Purple. ≥ 0.50 4. Wavelength (C = 0.003 G/L IN H2O). Extinction Coefficient. (615 +/- 4 NM). 17,000 ( ...
A few drops of starch solution are then added, and when the blue colour has nearly vanished a drop or two of methyl orange ... o-bromphenol, and o-phenoldisulphonic acid with potash, or, better, by heating its methyl ether, guaiacol, C 6 H 4 (OH) (OCH 3 ... which forms a blue compound with free iodine in iodometry; potassium chromate, which forms red silver chromate after all the ... and the end of the reaction is shown by the absence of a blue coloration when a drop of the test solution is brought into ...
4-Bromphenol. ; 4-bromofenol. ; 4-bromophenol(IUPAC). ; Phenol, 4-bromo-(CAS). ; Phenol, p-bromo-. ; p-Bromohydroxybenzene. ; p ... RO(a:HO,c:red)$color(&c)&a$color()@()-\-/`\`=,`\@RO(),`/\-0/-CH,`,@RO(OH),-CH2-@RO(NH2,blue) ... RO(a:HO,c:red)$color(&c)&a$color()@()-\-/`\`=,`\@RO(),`/\-0/-CH,`,@RO(OH),-CH2-@RO(NH2,blue) ...
  • the rate at which it migrates varies according to gel density and buffer composition, but in a typical 1% agarose gel in a 1X TAE buffer or TBE buffer, bromophenol blue migrates at the same rate as a DNA fragment of about 300 base pairs, in 2% agarose as 150 bp. (wikipedia.org)
  • 100 µg in 1 ml (100rxns) loading buffer (0.25% bromphenol blue, 0.25% xylene cyanol FF, 40% sucrose in water). (eenzyme.com)
  • In solution at pH 3.6 (in the middle of the transition range of this pH indicator) obtained by dissolution in water without any pH adjustment, bromophenol blue has a characteristic green red colour, where the apparent colour shifts depending on the concentration and/or path length through which the solution is observed. (wikipedia.org)
  • The method is based on the interaction of the drug base with bromophenol blue to give a stable ion-pair complex. (nih.gov)
  • Extractive spectrophotometric methods for determination of diltiazem HCl in pharmaceutical formulations using bromothymol blue, bromophenol blue and bromocresol green. (nih.gov)