A dye that has been used as an industrial dye, a laboratory indicator, and a biological stain.
Exclusive legal rights or privileges applied to inventions, plants, etc.
Determination, by measurement or comparison with a standard, of the correct value of each scale reading on a meter or other measuring instrument; or determination of the settings of a control device that correspond to particular values of voltage, current, frequency or other output.
The clear portion of BLOOD that is left after BLOOD COAGULATION to remove BLOOD CELLS and clotting proteins.
A basis of value established for the measure of quantity, weight, extent or quality, e.g. weight standards, standard solutions, methods, techniques, and procedures used in diagnosis and therapy.
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
A condition in which the FORAMEN OVALE in the ATRIAL SEPTUM fails to close shortly after birth. This results in abnormal communications between the two upper chambers of the heart. An isolated patent ovale foramen without other structural heart defects is usually of no hemodynamic significance.
An extracellular endopeptidase of vertebrate tissues similar to MATRIX METALLOPROTEINASE 1. It digests PROTEOGLYCAN; FIBRONECTIN; COLLAGEN types III, IV, V, and IX, and activates procollagenase. (Enzyme Nomenclature, 1992)
Compounds that bind to and block the stimulation of PURINERGIC P2 RECEPTORS.
Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.
DNA analogs containing neutral amide backbone linkages composed of aminoethyl glycine units instead of the usual phosphodiester linkage of deoxyribose groups. Peptide nucleic acids have high biological stability and higher affinity for complementary DNA or RNA sequences than analogous DNA oligomers.
A novel composition, device, or process, independently conceived de novo or derived from a pre-existing model.
Property, such as patents, trademarks, and copyright, that results from creative effort. The Patent and Copyright Clause (Art. 1, Sec. 8, cl. 8) of the United States Constitution provides for promoting the progress of science and useful arts by securing for limited times to authors and inventors, the exclusive right to their respective writings and discoveries. (From Black's Law Dictionary, 5th ed, p1014)
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A 9-kDa protein component of VERY-LOW-DENSITY LIPOPROTEINS and CHYLOMICRON REMNANTS. Apo C-III, synthesized in the liver, is an inhibitor of LIPOPROTEIN LIPASE. Apo C-III modulates the binding of chylomicron remnants and VLDL to receptors (RECEPTORS, LDL) thus decreases the uptake of triglyceride-rich particles by the liver cells and subsequent degradation. The normal Apo C-III is glycosylated. There are several polymorphic forms with varying amounts of SIALIC ACID (Apo C-III-0, Apo C-III-1, and Apo C-III-2).
A group of apolipoproteins that can readily exchange among the various classes of lipoproteins (HDL; VLDL; CHYLOMICRONS). After lipolysis of TRIGLYCERIDES on VLDL and chylomicrons, Apo-C proteins are normally transferred to HDL. The subtypes can modulate remnant binding to receptors, LECITHIN CHOLESTEROL ACYLTRANSFERASE, or LIPOPROTEIN LIPASE.
A disease of pregnant and lactating cows and ewes leading to generalized paresis and death. The disease, which is characterized by hypocalcemia, occurs at or shortly after parturition in cows and within weeks before or after parturition in ewes.
A class of lipoproteins of very light (0.93-1.006 g/ml) large size (30-80 nm) particles with a core composed mainly of TRIGLYCERIDES and a surface monolayer of PHOSPHOLIPIDS and CHOLESTEROL into which are imbedded the apolipoproteins B, E, and C. VLDL facilitates the transport of endogenously made triglycerides to extrahepatic tissues. As triglycerides and Apo C are removed, VLDL is converted to INTERMEDIATE-DENSITY LIPOPROTEINS, then to LOW-DENSITY LIPOPROTEINS from which cholesterol is delivered to the extrahepatic tissues.
Enzymes that catalyze the reversible reduction of alpha-carboxyl group of 3-hydroxy-3-methylglutaryl-coenzyme A to yield MEVALONIC ACID.
A condition of elevated levels of TRIGLYCERIDES in the blood.
Colloids with a solid continuous phase and liquid as the dispersed phase; gels may be unstable when, due to temperature or other cause, the solid phase liquefies; the resulting colloid is called a sol.
The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
A clear, odorless, tasteless liquid that is essential for most animal and plant life and is an excellent solvent for many substances. The chemical formula is hydrogen oxide (H2O). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
That portion of the electromagnetic spectrum in the visible, ultraviolet, and infrared range.
A repeat operation for the same condition in the same patient due to disease progression or recurrence, or as followup to failed previous surgery.
Automatic or hand operated equipment used to control and extinguish fires.
Devices for simulating the activity of the pancreas. They can be either electromechanical, consisting of a glucose sensor, computer, and insulin pump or bioartificial, consisting of isolated islets of Langerhans in an artificial membrane.
Organic chemistry methodology that mimics the modular nature of various biosynthetic processes. It uses highly reliable and selective reactions designed to "click" i.e., rapidly join small modular units together in high yield, without offensive byproducts. In combination with COMBINATORIAL CHEMISTRY TECHNIQUES, it is used for the synthesis of new compounds and combinatorial libraries.
The specialty of ANALYTIC CHEMISTRY applied to assays of physiologically important substances found in blood, urine, tissues, and other biological fluids for the purpose of aiding the physician in making a diagnosis or following therapy.
The study of the structure, preparation, properties, and reactions of carbon compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
A 90-kDa protein produced by macrophages that severs ACTIN filaments and forms a cap on the newly exposed filament end. Gelsolin is activated by CALCIUM ions and participates in the assembly and disassembly of actin, thereby increasing the motility of some CELLS.
Rapid methods of measuring the effects of an agent in a biological or chemical assay. The assay usually involves some form of automation or a way to conduct multiple assays at the same time using sample arrays.
Nanometer sized fragments of semiconductor crystalline material which emit PHOTONS. The wavelength is based on the quantum confinement size of the dot. They can be embedded in MICROBEADS for high throughput ANALYTICAL CHEMISTRY TECHNIQUES.
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
Monomeric subunits of primarily globular ACTIN and found in the cytoplasmic matrix of almost all cells. They are often associated with microtubules and may play a role in cytoskeletal function and/or mediate movement of the cell or the organelles within the cell.
A heterotrimeric DNA-binding protein that binds to CCAAT motifs in the promoters of eukaryotic genes. It is composed of three subunits: A, B and C.
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
Electrophoresis in which agar or agarose gel is used as the diffusion medium.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current.
Electrophoresis in which a second perpendicular electrophoretic transport is performed on the separate components resulting from the first electrophoresis. This technique is usually performed on polyacrylamide gels.
Gel electrophoresis in which the direction of the electric field is changed periodically. This technique is similar to other electrophoretic methods normally used to separate double-stranded DNA molecules ranging in size up to tens of thousands of base-pairs. However, by alternating the electric field direction one is able to separate DNA molecules up to several million base-pairs in length.

Procedure for calibrating the Technicon Colorimeter I. (1/29)

We describe a rapid method for calibrating the Technicon AutoAnalyzer colorimeter I. Test solutions of bromphenol blue are recommended for the calibration, in preference to solutions of potassium dichromate, based on considerations of the instrument's working range and of the stray light characteristics of the associated filters.  (+info)

Guidance for selecting the measurement conditions in the dye-binding method for determining serum protein: theoretical analysis based on the chemical equilibrium of protein error. (2/29)

A methodology for selecting the measurement conditions in the dye-binding method for determining serum protein has been studied by a theoretical calculation. This calculation was based on the fact that a protein error occurs because of a reaction between the side chains of a positively charged amino acid residue in a protein molecule and a dissociated dye anion. The calculated characteristics of this method are summarized as follows: (1) Although the reaction between the dye and the protein occurs up to about pH 12, a change in the color shade, called protein error, is observed only in a pH region restricted within narrow limits. (2) Although the apparent absorbance (the absorbance of the test solution measured against a reagent blank) is lower than the true absorbance indicated by the formed dye-protein complex, the apparent absorbance correlates with the true absorbance with a correlation coefficient of 1.0. (3) At a higher dye concentration, the calibration curve is more linear at a higher pH than at a lower pH. Most of these characteristics were similarly observed experimentally in the reactions of BPB, BCG and BCP with human and bovine albumins. It is concluded that in order to ensure the linearity of the calibration curve, the measurement should be performed at a higher dye concentration and sufficiently high pH where the detection sensitivity is satisfied.  (+info)

Microfluidic large-scale integration. (3/29)

We developed high-density microfluidic chips that contain plumbing networks with thousands of micromechanical valves and hundreds of individually addressable chambers. These fluidic devices are analogous to electronic integrated circuits fabricated using large-scale integration. A key component of these networks is the fluidic multiplexor, which is a combinatorial array of binary valve patterns that exponentially increases the processing power of a network by allowing complex fluid manipulations with a minimal number of inputs. We used these integrated microfluidic networks to construct the microfluidic analog of a comparator array and a microfluidic memory storage device whose behavior resembles random-access memory.  (+info)

Preparation of sand fly (Diptera: Psychodidae: Phlebotominae) specimens for histological studies. (4/29)

Phlebotominae sand fly specimens were prepared for histological and physiological studies. Different fixatives were tested on sectioned and whole bodied adult females in order to obtain good fixation and provide satisfactory penetration of the embedding media. All fixed specimens were infiltrated (up to seven days under 5 C) and embedded in hydroxyethyl metacrylate. Two-three m sections were stained, mounted in Canada balsam and observed by light microscopy. Best results were achieved when whole bodied insects were double fixed in Bouin's and Carnoy's fluids (4 h/2 h) and stained in Hematoxilin/Eosin or fixed in calcium formaldehyde and stained in mercury bromophenol blue.  (+info)

Purification and characterization of an aflatoxin degradation enzyme from Pleurotus ostreatus. (5/29)

Nineteen fungi were tested for their ability to degrade aflatoxin B1 (AFB1). An extracellular enzyme from the edible mushroom Pleurotus ostreatus showed afaltoxin-degradation activity detected by thin-layer chromatography (TLC). An enzyme with this activity was purified by two chromatographies on DEAE-Sepharose and Phenyl-Sepharose. The apparent molecular mass of the purified enzyme was estimated to be 90 kDa by SDS-PAGE. Optimum activities were found in the pH range between 4.0 and 5.0 and at 25 degrees C. Also, degradation activity of several dyes in the presence of H2O2 was tested, resulting in the detection of bromophenol blue-decolorizing activity. Based on these data, we suggest this enzyme is a novel enzyme with aflatoxin-degradation activity. Fluorescence measurements suggest that the enzyme cleaves the lactone ring of aflatoxin.  (+info)

Delivery of several forms of DNA, DNA-RNA hybrids, and dyes across human sclera by electrical fields. (6/29)

PURPOSE: Iontophoresis has been used for drug delivery across the cornea for many years. We sought to test whether small charged dyes and DNA can be transferred across human sclera by an electric field. METHODS: Full-thickness human scleral fragments were embedded vertically in an agarose gel and positioned to completely span individual gel lanes. The scleral fragments were located approximately 1 cm downstream from the gel wells. DNA or dyes were loaded into the wells and electrophoresis was carried out at about 3.3 V/cm for approximately 2 h per run. Movement of DNA and dyes through the agarose and sclera was measured with either digital time-lapse photography or through DNA extraction and purification from the gel. SYBR green stain was used as a sensitive method to detect DNA. RESULTS: Digital time-lapse photography of agarose gel electrophoresis revealed that two dyes, xylene cyanol and bromphenol blue, passed through the sclera in the presence of an electric field. Xylene cyanol was driven through the sclera virtually unimpeded except for some spreading of the dye. Bromphenol blue was slowed markedly by the sclera, but it too eventually passed through the tissue. Small DNAs, including a single stranded 51-mer and a double hairpin 68-mer oligonucleotide, passed through the sclera as detected by SYBR green staining. Linear double stranded DNAs ranging from 50 bp to 12,000 bp passed through the sclera. The larger the DNA, the slower the rate of passage through the sclera, and the greater the band spreading. pEGFP-1 (a 3 kb plasmid) passed through the sclera but was accompanied by a great amount of band spreading. Following completion of the initial electrophoresis run, the plasmid DNA was extracted from the smeared bands in the agarose distal to the sclera and re-run on a second gel without sclera. The initially smeared plasmid bands resolved into 2 distinct bands after extraction and purification and matched well with control plasmid bands. CONCLUSIONS: Charged molecules such as xylene cyanol, bromphenol blue, and DNAs ranging from 51 bp oligonucleotides to a 3 kb plasmid can be driven across human sclera by an electric field and directly detected. Passage of plasmids was efficient, but the plasmid bands were diffuse after transit. This technique offers promise as a noninvasive DNA delivery tool, where gene therapy can be accomplished by small RNA or DNA synthetic oligonucleotides, larger double stranded fragments, or even plasmids.  (+info)

Theoretical analysis concerning the characteristics of a dye-binding method for determining serum protein based on protein error of pH indicator: effect of buffer concentration of the color reagent on the color development. (7/29)

In the dye-binding method based on protein error of a pH indicator, the color development has been reported to be markedly affected by the buffer concentration of the color reagent. In this study, the author analyzed this phenomenon by a theoretical calculation based on the chemical equilibrium of protein error. The calculation was performed on the assumption that both the dissociated dye anion and the anion contained in the buffer solution react with protein, forming a dye-protein complex and an anion-protein complex, respectively. The calculated results were compared with those obtained by the experiments using bromophenol blue, bromocresol green and bromocresol purple that are employed widely for determining the human serum albumin concentration clinically. The calculated results of this method are summarized as follows: (1) the color development decreases with the increase in the concentration of the anion contained in the buffer solution; (2) the calibration curve is more linear in the higher concentration of the anion than in the lower one. These calculated results agreed well with the experimental ones. From these results, it was concluded that the change in the color development by the buffer concentration of the color reagent is due to the change in the concentration of the buffer anion.  (+info)

An evaluation of novel vital dyes for intraocular surgery. (8/29)

PURPOSE: To evaluate systematically the staining characteristics and safety of potential new dyes for intraocular surgery. METHODS: Six dyes were included in the investigation: light green SF (LGSF) yellowish, E68, bromophenol blue (BPB), Chicago blue (CB), rhodamine 6G, rhodulinblau-basic 3 (RDB-B3). All dyes were dissolved and diluted in a balanced saline saline solution. The light-absorbing properties of each dye were measured at a concentration of 0.05% between 200 and 1000 nm. Staining characteristics were examined by staining lens capsule tissue and epiretinal membranes (ERMs), removed intraoperatively, with dye concentrations of 1.0%, 0.5%, 0.2%, and 0.05%. Enucleated porcine eyes (postmortem time, 9 hours) were also stained. Dye-related toxicity was evaluated by a colorimetric test (MTT) measuring the inhibition of retinal pigment epithelium (RPE) cell proliferation (ARPE-19 and primary human RPE cells, passages 3-6). Cell viability was also quantified based on a two-color fluorescence cell-viability assay. Dyes were investigated in concentrations of 0.2% and 0.02%. RESULTS: All dyes investigated in this study stained human lens capsules, removed intraoperatively; ERMs, peeled during macular pucker surgery; and enucleated porcine eyes, depending on the concentration applied. The long-wavelength absorption maximum of the dyes was within the range of 527 to 655 nm at concentrations of 0.05%. Rhodamine G6 and RDB-B3 showed adverse effects on ARPE-19 cell proliferation at a concentration of 0.2% and were excluded from further investigation in primary RPE cells. The remaining four dyes showed no toxic effect on ARPE-19 and primary RPE cell proliferation at concentrations of 0.2% and 0.02%. Cell viability was affected by LGSF yellowish (0.2%) and CB (0.2% and 0.02%). Two dyes (E68 and BPB) showed no relevant toxicity in vitro. CONCLUSIONS: The systematic evaluation of dyes for intraocular use seems mandatory. In this study four dyes were identified with effective staining characteristics, with two of these dyes having no detectable toxic effect on RPE cells in vitro.  (+info)

Often a side-effect of pregnancy, lots of moms need to have. gallbladder attack, these are decent rules to follow in the game of dietary life. Its simple, and yet we all struggle with it so much. Keep tempting foods out of the house, stay.. When you have an upset stomach from too much acid build up, Alka-Seltzer can act as a buffer. This experiment shows. it can feel upset. Alka-Seltzer is a buffer which keeps your stomach from being too acidic. This demonstration using bromphenol blue and vinegar, will show how Alka-Seltzer neutralizes stomach acid.. If you do NOT, you probably have low stomach acid. Stop the Thyroid Madness was one of the 2016 Winner Blogs. far below what we have to pay every month.. HCL Acid in Stomach , Healthy Eating , SF Gate - Once you start eating, your body produces a strong gastric acid called hydrochloric acid, or HCL, to begin the process of stomach digestion. During this process.. Introduction; Food is Complex and Contains Many Types of Molecules; Proteins ...
Proteins were separated by standard 2-DE. Briefly, the first dimension was performed in IPGphor™ (GE Healthcare, UK) isoelectric focusing (IEF) apparatus. Linear, 24 cm long, pH 4-7 Immobiline™ DryStrip gels (IPG-strips, GE Healthcare, UK) were rehydrated in the strip holders for 4 hours in 0.45 ml rehydration buffer containing 9 M urea, 2% (w/v) CHAPS, 0.5% (v/v) IPG-buffer pH 4-7, 1.2% (v/v) DeStreak™ reagent, a trace of bromophenol blue and 150 μg of total amount of protein. IEF was carried out at +20°C using following step-and-hold settings: 50 V, 8 h; 100 V, 1 h; 500 V, 1 h; 1000 V, 1 h; 2000 V, 1 h; 8000 V, until 95000 Vh was achieved. Then, the IPG-strips were incubated at room temperature in equilibration buffer (50 mM Tris-HCl, pH 8.8, 6 M urea, 30% (v/v) glycerol, 2% (w/v) SDS, a trace of bromophenol blue, and 10 mg/ml DTT) for 15 min and for another 15 min in the same buffer that contained 25 mg/ml of iodoacetamide instead of DTT. The second-dimension separation was performed ...
Marian Blanca Ramírez from the CSIC in Spain has been studying the effects of LRRK2, a protein associated with Parkinsons disease, on cell motility. A Travelling Fellowship from Journal of Cell Science allowed her to spend time in Prof Maddy Parsons lab at Kings College London, learning new cell migration assays and analysing fibroblasts cultured from individuals with Parkinsons. Read more on her story here. Where could your research take you? The deadline to apply for the current round of Travelling Fellowships is 23rd Feburary 2018. Apply now!. ...
Gentaur molecular products has all kinds of products like :search , Arbor \ Color Reagent B, 5ML \ X093-5ML for more molecular products just contact us
Mouse/Rat Anti-Canine CD3 / CD4 Dual Colour Reagent Antibody, FITC / R-Phycoerythrin Conjugated from AbD Serotec,MSE ANTI CAN. CD3:FITC/RAT ANTI CD4:RPE,biological,biology supply,biology supplies,biology product
The denaturation of bovine serum albumin (BSA) by guanidine hydrochloride (GdnHC1) showed a single-step, two-state transition, when monitored by different probes such as intrinsic fluorescence at 338 and 333 nm after excitation at 280 and 295 nm respectively, UV difference spectral signal at 288 nm, 1-anilinonaphthalene-8-sulphonate (ANS) fluorescence at 470 nm after excitation at 380 nm, bromophenol blue (BPB)-induced difference spectral signal at 619 nm and A.,„„ of positive difference spectral signal of BPB-BSA complex. A comparison of the denaturation curves obtained with the above mentioned probes showed differences in the requirement of GdnHC1 concentration• for the transition to start and complete. The values for the mid-point of denaturation transition and free energy change associated with GdnHC1 denaturation (AG Du2o.) also varied from each other, using different probes ...
SDS-PAGE abnormalities in proteins near to front dye - posted in -Biochemistry-: Hi, I want to get some helps from all of you... I have done a lot of SDS-PAGE with strange migration of protein near to front dye. In a normal condition, the smallest protein marker should travel a bit slower than the protein samples migration indicator (bromophenol blue), i get a nice result last time (as shown in normal.png) but after i have prepared a new solution of acrylamide, the condition com...
1) Centrifuge sample containing hydrogels and crystal violet indicator 3 times (5 minutes, 13200 rpm) -Measure the absorbance of each supernatant to find the amount of CV that the hydrogels have absorbed 2) Prepare 2 more hydrogel solutions with the following indicators (these will be used to measure dye leakage as well as to monitor pH changes) a) methyl red (55μL) in 1.5 mL ethanol w/ 2mg hydrogels b) bromophenol blue (30μL) in 1.5 mL water w/ 2mg hydrogels ...
For whole cell protease treatment method, E. coli cells have been harvested, washed and resuspended in one ml Tris HCl. Proteinase K was additional to final concentrations in between 0. two mg mL 1 and 0. 5 mg mL 1 and cells had been incubated for one hour at 37 C. Digestion was stopped by washing the cells twice with Tris HCl containing 10% fetal calf serum and outer membrane proteins were ready as described over. For outer membrane proteins that have been applied for ac tivity assays, cells werent handled with Proteinase K. SDS Page Outer membrane isolates were diluted with sam ple buffer containing 4% SDS, 0. 2% bromophenol blue, 200 mM dithiothreitol and 20% glycerol boiled for 10 minutes and analyzed on 10% polyacrylamid gels. Proteins had been stained with Coomassie brilliant blue.. To correlate molecu lar masses of protein bands of curiosity, a molecular fat conventional was employed. Flow cytometer evaluation E. coli BL21 pAT kinase inhibitor MLN0128 LipBc cells were grown and ex ...
The ventricular tissues were suspended in 40 ml of ice-cold 10% tricholroacetic acid and homogenized with a tissue homogenizer (2 bursts, 30 s each). Homogenates were centrifuged at 10,000 g for 10 min at 4°C, and protein content was determined using detergent-compatible protein assay (Bio-Rad Laboratories Inc., Hercules, CA) with bovine serum albumin. The proteins were then put in a 3× sample buffer consisting of 0.2 M Tris-HCl (pH 6.8), 4% sodium dodecylsulfate, 8 M urea, 0.1 M dithiothreitol, and 0.01% bromophenol blue. Equal amounts of protein per lane were loaded onto a 15% polyacrylamide gel and separated by electrophoresis at 30 mA/gel for 60 min with a running buffer containing 25 mm Tris, 192 mm glycine, and 0.1% sodium dodecylsulfate. Molecular weight markers (Amersham Biosciences, Buckinghamshire, United Kingdom) were used in each gel. Proteins were transferred to a polyvinylidene diflouride membrane (Immobilon-P; Millipore Corp., Bedford, MA) at 36 V for 4 h using a transfer buffer ...
This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 100 mM DTT).. ...
This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol ...
This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol ...
You dont need the Kit - Just go to you local chemical store and get some concentrated HCL to make your soln, Bromophenol blue, and a 5 ml syringe or 5 ml pipet with pipet pump. I would go with the pipet since you will end up going throught a number of syringes. You can get the hardware on the internet but buy the chemicals local since they charge you an arm and a leg to ship chemicals. Thats what I did and it is all fairly basic. The hardest part is making the .01 M HCL soln - just follow girlmarks instructions poster earlier. Oh, a stiring rod helps too or a mag stirer if you want to get fancy ...
HEPG2SP (hepatoblastoma carcinoma derived cell line secreted proteins): Hundred ml of supernatant HEPG2 culture media were concentrated down to 100 l in a MicrosepTM Concentrators. The concentrated sample was mixed with 400 l of a solution containing urea (8 M), CHAPS (4% w/v), DTE (65 mM), Resolytes 3.5-10 (2 % v/v) and a trace of bromophenol blue. The whole final diluted HEPG2SP sample was used for in-gel sample rehydration. NAME=1001363,HL60 (promyelocytic leukemia cells): A monolayer culture of a human promyelocytic leukemia cell line was grown in Dulbeccos modified Eagle medium (DMEM) containing 10% fetal calf serum (FCS). Cells were rinsed once with DMEM without FCS and removed from the flask by incubating them with a solution containing trypsin (0.5 g/l) and EDTA (0.2 g/l). After 3 minutes, DMEM containing FCS was added into the flask to stop the action of the trypsin. The cells were detached from the surface of the flask by squirting the solution onto the cells. The suspension was ...
Whole-cell extracts were prepared from cultured cell monolayers as follows: the culture medium was drained off the cells, and the adherent cells were washed twice with ice-cold PBS. The cells were lysed with ice-cold modified RIPA buffer (50 mM Tris-HCl (pH 7.4), 1% NP-40, 0.25% Na-deoxycholate, 150 mM NaCl, and 1 mM EDTA) containing protease inhibitor cocktail (Sigma-Aldrich). The cell suspension was transferred into a centrifuge tube, left on ice, and vortexed every 5 minutes, for 15 minutes to lyse the cells. The lysate was then centrifuged at 14,000g in a precooled centrifuge for 15 minutes. The supernatant was collected and the concentration of protein in extracts was determined by using a protein assay concentrate (Bio-Rad, Hercules, CA). Protein samples (10 μg) were dissolved in sample buffer (100 mM Tris [pH 6.8], 4% SDS, 20% glycerol, 0.02% bromophenol blue, and 50 μL/mL β-mercaptoethanol) at a concentration of 1:1. Samples were resolved by SDS-PAGE according to the method of Laemmli ...
Nucleic acid: RNA, single-stranded (G 22.8; A 26.4; C 30.3; U 20.5), with an apparent M. Wt of 2.2-2.6 x 106 in slab gel electrophoresis (Fig.6) under partially denaturing conditions as described by Gould (1981) (2.6% acrylamide, 0.17% methylene bisacrylamide, 7 M urea, 90 mM Tris-borate, 3 mM sodium ethylenediamine-tetraacetate, pH 8.3) (G. Boccardo, unpublished data). Protein: Purified virus particles resuspended in 6 M urea, 2% (w/v) 2-mercaptoethanol, 1% (w/v) sodium dodecyl sulphate and 0.001% (w/v) bromophenol blue in 0.125 M Tris-HCl, pH 6.8, yielded electrophoretically homogeneous subunits (Fig.5) of M. Wt 2.1 x 104 (mean of 14 determinations, s.e. = 0.1 x 104) (G. Boccardo, unpublished data). M. N. Short (personal communication) calculated a M. Wt of 21,473 based on 204 amino acid residues (lys 5, his 1, arg 9, asp 14, thr 25, ser 17, glu 18, pro 17, gly 14, ala 27, half cys 2, val 8, met 1, ile 7, leu 24, tyr 3, phe 10, trp 2).. ...
You can indeed use the FFA as additional feedstock. I have done that too, I typically just mix the FFA 50/50 with the WVO and dewater/titrate/process. The key is making sure the FFA is clean enough to process. You really need to make sure that you get good separation, otherwise your FFA layer is polluted with salts. You can do a soap titration with bromophenol blue to figure out how much acid is need to completely nuetralize the FFA. I usually just take a few half liter samples, add different quantities of sulfuric and determine the winning amount. Typically 3%-5% by volume sulfuric ...
The present invention relates to immunopharmacology and, more specifically, to a preparation controlling the T-system of immunity and to a method for producing the same.The preparation controlling the T-system of immunity is characterized by its content as the active principle, peptides with a molecular mass of from 1,500 to 6,000 Dalton having absorption maximum in UV-light at 280 and 275 nm and electrophoretic mobility in a polyacrylamide gel relative to bromophenol blue: 0.062-0.102; 0.156-0.236; 0.354-0.374; 0.382-0.422; 0.432-0.472; 0.485-0.545; 0.850-0.930.The method for producing the preparation according to the present invention comprises homogenization of a tissue of thymus gland in a solution of sodium chloride; the homogenizate is kept for 12-16 hours at a temperature of from 2.degree. to 6.degree. C., the residue is removed, thermolabile proteins are removed from the solution, peptides and proteins are precipitated and then dissolved and
GAPDH from different species.. 1 x 1 mg vial, in 50% PBS, pH 7.4, 50% glycerol.. Antigen Standard is ready to use after reconstitution in distilled water.. The vial contains 5 µg of human GAPDH. Add 50 µl of water per vial and dissolve the pellet with gentle stirring. After dissolving centrifuge the vial briefly to collect all the liquid to the bottom of the vial.. 1 x 5 µg vial, lyophilized from protein solution in 62.5 mM Tris-HCl, pH 6.8, 2% SDS, 10% sucrose, 1% 2-mercaptoethanol, 0.1% bromophenol blue. ...
Unit 8 Chemical Equilibrium Focusing on Acid-Base Systems unit 8Chemical Equilibrium Chemical Equilibrium Focusing on Acid Base Systems Equilibrium describes any condition or situation of balance. We recognize
We use cookies to ensure that we are offering you the best user experience on our website. By continuing, you confirm that you accept our policy statement ...
Scientists have discovered that when cells are confronted with an invading virus or bacteria or exposed to an irritating chemical, they protect themselves by going off their DNA recipe
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice ...
View Notes - Equilibrium 2010 from CHEM 152 at Pima CC. Chemical Kinetics and Equilibrium Part 2: Chemical 2: Chemical Equilibrium David A. Katz Department of Chemistry Pima Community College Tucson,
In this paper we describe an approach for teaching the relation between chemical equilibrium and free energy that is, according to our experience, very efficient and enlightening. This approach has a strong visual appeal and can be used at different levels, from simple presentation of the results as graphs of free energy versus reaction mixture composition, up to full derivation of formulas to find the equations of the curves ...
A remarkable nano-confinement entropic effect on chemical equilibrium (NCECE) was predicted by us using a novel lattice-gas based statistical-mechanical modeling.
It is shown that chemical equilibrium is reached both in homogeneous and heterogeneous area of composition of reactive mixture. The liquid - liquid equilibrium data for the surface of chemical equilibrium were obtained. The thermodynamic constants of chemical equilibrium at 303.15 and 313.15 K were determined. © 2017 Elsevier B.V. ...
25 μL of sample is required for each assay. Specimens, calibrators and controls are pre-diluted 1:21 prior to placing them in the test wells. Cut-off calibrator, controls and subject samples are diluted according to the specific test kit protocol, mixed briefly by vortex to assure good distribution of the sample in the diluent, and then transferred to the appropriately labeled wells and incubated. After washing the wells, conjugate is added with another incubation and wash step following. Substrate is added and allowed to incubate before the addition of stop/color reagent. The plates are read at an absorbance of 450 nm using a reference wavelength of 630 nm. EIA indices are calculated by hand using the formulas in the kit package insert. Data are recorded (1) as Positive (EIA index ≥ 1.10), Negative (EIA index ,0.90), or Equivocal (EIA index ≥ 0.90 to 1.09), and (2) as the exact numeric EIA index. The sensitivity of the assay is 96.6% and the specificity is 97.7%. ...
7). Eq (7) can be used to determine the amount of biomass required for any given initial dye concentration and for any desired amount of dye removal for any multistage system.. For a two stage batch sorption system, the design parameters are now explained. The design objective is to treat 50 L of basic red 9 solution of initial dye concentration 150 mg/L in the first stage. A series of equilibrium dye concentration from 140 mg/L to 10 mg/L in 10 decrements was considered in stage one of a two stage sorption system. The design plot which explains the amount of biomass needed in different two stage sorption systems are shown in Figure 3. The x-axis in Fig 3 represents the equilibrium concentration in the first stage of the two stage sorption system based on 10 mg/L of equilibrium dye concentration difference. In the sorption system number one, the design the objective is to reduce the initial dye concentration from 150 mg/L to 140 mg/L. Similarly in the sorption system 2, 3, 4, 5, 6, 7, 8, 9, 10, ...
In stoichiometry calculations, we assume that reactions run to completion. However, when a chemical reaction is carried out in a closed vessel, the system achieves equilibrium. Equilibrium occurs when there is a constant ratio between the concentration of the reactants and the products. Different reactions have different equilibria. Some may appear to be completely products, however, all reactions have some reactants present. A reaction may look finished when equilibrium is reached, but actually the forward and reverse reactions continue to happen at the same rate. A reverse reaction is when the written reaction goes from right to left instead of the forward reaction which proceeds from left to right. This is why equilibrium is also referred to as steady state.. It is possible to write an equilibrium expression for a reaction. This can be expressed by concentrations of the products divided by the concentration of the reactants with the coefficients of each equation acting as exponents. It is ...
solubility equilibria notes, Jan 26, 2020 · Equilibrium Chemistry Class 11 Notes. These are Chemical Equilibrium revision notes of Equilibrium Chemistry Class 11 Notes. These notes provided for study purpose by ChemistryNotesInfo.com website. CHEMICAL EQUILIBRIUM . Chemical reaction occur in one direction from reactant to products are termed as irreversible chemical reactions.
A worked example using reaction quotient Q to predict how concentrations will shift so the reaction can get to equilibrium. Watch the next lesson: htt...
Close The Infona portal uses cookies, i.e. strings of text saved by a browser on the users device. The portal can access those files and use them to remember the users data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser. ...
Introduction. ________________ 2.0 Abstract: From the experiment, we become understand about dynamic equilibrium and Le Chatelier?s principle. Besides, we able to observe the change of an equilibrium when the concentration of a reactant or product is altered. We also know how to predict the effect of concentration changes on chemical equilibrium. This report shows, whenever a system in equilibrium is disturbed the system will adjust itself in such a way that the effect of the change will be reduced or moderated. Changing the concentration of a chemical will shift the equilibrium to the side that would reduce that change in concentration. The chemical system will attempt to partly oppose the change affected to the original state of equilibrium. In turn, the rate of reaction, extent, and yield of products will be altered corresponding to the impact on the system. The principle is used to manipulate the outcomes of reversible reactions, often to increase the yield of reactions. 3.0 Introduction: ...
Lab O6 - Equilibrium Lab Application Purpose: An introduction to the qualitative aspects of chemical equilibrium. Starting with a reaction at equilibrium, one will change the concentration of various ions present in the equilibrium, and record the states of the changing equilibrium through observation.
022m. A colour camera recorded depth integrated images at 25 frames see more per second which were then time averaged over a period of 7 s. Fig. 4a shows an image of a jet containing passive dye and provides information about the depth integrated and time averaged dye concentration CDI(x,y)CDI(x,y). An inverse Abel transformation (Abel, 1826) was performed to reconstruct the axisymmetric form of the dye concentration through the jet using equation(17) C‾(x,z)=-1π∫r∞dC‾DIdmdmm2-r2.Fig. 4b shows the reconstructed concentration profile.. It has been known that the time averaged concentration field C‾ across the jet is approximately Gaussian (e.g. Morton et al. (1956), etc.) i.e. equation(18) C‾=C‾01+(2αy/b0)exp-λx2b2. The dilution at any location in the jet D(x,y)D(x,y) can be estimate by relating the centre line concentration C to the value at the nozzle C0C0 and radius b to the value that captures 95% of the jet fluid giving λ=log(1/0.05)≃3λ=log(1/0.05)≃3. This relationship ...
It contains a pH indicator , bromothymol blue, which causes the medium to change from yellow to blue green; it contains antimicrobial agents, chloramphenicol and chlortetracycline, and an … Inoculate the medium immediately after urine collection. The decrease in the fluorimetric signal obtained after a two-step derivatization reaction involving choline oxidase and horseradish peroxidase is proportional to the inhibition caused by the analyte and hence to its concentration in the sample. It is mostly used in applications that require measuring substances that would have a relatively neutral pH (near 7). Bromothymol blue is the most commonly used pH indicator and is in low concentration and size container and low toxicity. Bromophenol blue is structurally related to phenolphthalein (a popular indicator). Ensure adequate ventilation. Bromphenol Blue - Use and Manufacturing. Potassium ions can be precipitated by tetraphenylborate. [7] Bromophenol blue is the substance with the highest known value ...
Two novel chemical sensing systems using thin organic films have been elaborated and compared, one involving well established colour reagents used with a novel piezo-optical transduction system and the other using alkylviologen films for molecular recognition of phenols, with transduction via surface plasmon resonance (SPR) techniques. In the piezo-optical technique, chopped light absorbed by the thin sensing film deposited on piezoelectric polyvinylidene fluoride (PVDF) is converted into heat by non-radiative decay. This expands the film, stressing the PVDF and generating an electric charge which is measured using a lock-in amplifier. The signal dependence on optical absorption length, thermal diffusion length, film uniformity and porosity, chopper frequency and amplifier phase synchronisation are reviewed. The design and selection of molecular receptors for phenols, and the fabrication of thin films suitable for SPR, are described together with results demonstrating response patterns to ...
Clonidine hydrochloride is an antihypertensive agent used for migraine prophylaxis, attention deficit hyperactivity disorder, menopausal flushing and Tourette syndrome. The quantity of the active substance in pharmaceutical preparations must be within specific limits, in agreement with the respective label claim. Therefore, the aim of this study was to establish the conditions for two spectrophotometric methods for clonidine determination, based on the formation of the ion pair complex between clonidine hydrochloride and thymol blue/bromophenol blue. A Jasco UV-Vis 530 spectrophotometer was used for the analysis and the maxim absorbance was measured at 418 nm/448 nm against blank solution. After validation, the methods were used for quantification of clonidine hydrochloride in two commercial samples (tablets). The recovery of active substance varies between 98.06 and 100.13 % without interferences from the excipients.
0189] In order to allow for the use of an alkyl lithium in the synthetic route the 2-bromophenol of general formula 2 is protected. A large variety of phenol protecting groups that are stable in the presence of alkyl lithiums could be employed, for example those described in Wuts, P. G. M. and Greene, T. W., Greenes Protective Groups in Organic Synthesis 4th Ed., Wiley & Sons, 2007. In accordance with the preferred embodiment of this invention, the 2-bromophenol of general formula 2 is protected as a methyl ether, methoxymethyl ether (MOM ether) or methoxyethoxymethyl ether (MEM ether). Reaction conditions to achieve the desired phenol protection are described in Wuts, P. G. M. and Greene, T. W., Greenes Protective Groups in Organic Synthesis 4th Ed., Wiley & Sons, 2007. In accordance with the preferred embodiment of this invention, the methyl ether of general formula 8 (where P═CH3) is prepared by treatment of the 2-bromophenol of general formula 2 with potassium carbonate and iodomethane ...
To know the exact diagram represent the effect of catalysis on chemical equilibrium, I searched on experimental data but I cant find suitable data. so I consider a hypothetical reversible first -order reaction in each direction where $$\ce{ A ,=, B}$$ I choose those two equations for reversible first- order reaction to calculate the concentrations of product and reactant at a different time: $$\ce{[A]}=\frac{k_b +K_fe^{-(k_f+k_b)t} }{k_f+k_b}{[A]_0}$$ $$\ce{[B]}=\frac{k_f -K_fe^{-(k_f+k_b)t} }{k_f+k_b}{[A]_0}$$. Starting with A at a concentration$[A]_0={0.6}$ and the values of the rate constants of uncatalayzed reaction are $k_f=3s^{-1}$ and $k_b=1s^{-1}$. and the values of the rate constants of catalayzed reaction are $k_f=6s^{-1}$ and $k_b=2s^{-1}$. I did the calculation using excel sheet and draw the graph of concentration Vs time : ...
Equilibrium chemistry is concerned with systems in chemical equilibrium. The unifying principle is that the free energy of a system at equilibrium is the minimum possible, so that the slope of the free energy with respect to the reaction coordinate is zero. This principle, applied to mixtures at equilibrium provides a definition of an equilibrium constant. Applications include acid-base, host-guest, metal-complex, solubility, partition, chromatography and redox equilibria. A chemical system is said to be in equilibrium when the quantities of the chemical entities involved do not and cannot change in time without the application of an external influence. In this sense a system in chemical equilibrium is in a stable state. The system at chemical equilibrium will be at a constant temperature, pressure (or volume) and composition. It will be insulated from exchange of heat with the surroundings, that is, it is a closed system. A change of temperature, pressure (or volume) constitutes an external ...
Le Chateliers Principle can be used to predict how a change in condition affects a chemical equilibrium. Essentially, if there is a change in either the concentration of reactants/products, temperature, volume or pressure, the chemical system will try to respond by counteracting this change and establishing a new chemical equilibrium. This principle can be illustrated in the Haber process:. N2 + 3H2 ,-, 2NH3. For example, if the concentration of NH3 decreased suddenly, the system would try to counteract this change by increasing the concentration of the product. It can do this by shifting the equilibrium of the reaction to the right, by essentially using more reactants to turn into product.. Such a principle can and has been manipulated in chemical industries, such as in the above example, to improve the yield of a certain product. So by removing NH3 continuously, the chemical equilibrium will respond to this by generating more ammonia. This ammonia is then removed, and the system generates ...
The BD OneFlow™ ALOT (Acute Leukemia Orientation Tube) is a pre-configured single-dose, ready-to-use 8-color reagent. Two tubes, one containing the cytoplasmic markers (C tube) and one containing the surface markers (S tube), provide a single-test.. The BD OneFlow ALOT is intended for flow cytometric immunophenotyping of aberrant immature populations of hematopoietic cells (lymphoid and non-lymphoid lineage) in bone marrow and peripheral blood as an aid in the diagnosis of acute lymphoblastic leukemia and non-lymphoid acute leukemia.. As a screening tube, the BD OneFlow ALOT can guide the need for further analysis in combination with panel(s) specifically designed for the classification of different forms of B, T and myeloid acute leukemias.. The BD OneFlow ALOT is available in the 10 test/box size (4 pouches of 5 tubes each: 2 pouches of S tubes and 2 pouches of C tubes).. Dark red color-coded boxes, pouches and tubes allow for easy visual identification.. The reagent composition is shown ...
Attempts have been made to synthesise a novel siloxane-containing dicyanate ester, 1,3-bis(4-cyanatophenyl)-1,1,3,3-tetramethyldisiloxane, commencing from 4-bromophenol or 4-methoxyphenol. Most of these syntheses have involved the use of a Grignard reaction to form a disiloxane group, followed up subsequent cleavage of an alkyl-protecting group to yield a free phenol. Several different ethers have been attempted, but none was successful. The extreme susceptibility of the aryl silicon-carbon bond to cleavage under acidic conditions has been demonstrated by these reactions. A novel phosphazene-containing dicyanate ester, 1,3-bis(4-cyanatophenyl)-1,3,5,5-tetraphenoxycyclotriphosphazene has been successfully synthesised in a four-step procedure with an overall yield of ca. 35%. The cyanate ester has been characterised by differential scanning calorimetry (DSC), thermogravimetric (TG) analysis, thermomechanical analysis (TMA), 1H, 13C and 13P NMR spectroscopy, and Fourier-Transform Infra-red (FT-IR) ...
The solubility of a two-component, H 2 O+CO 2 fluid in silicate liquids was modeled by assuming mechanical, thermal, and chemical equilibrium between the fluid and liquid phases. The liquid phase was treated as a 12-component (10 major oxides+H 2 O+CO 2 ) mixture, and its thermodynamic properties were calculated on the basis of a regular and non-isometric mixing equation for the excess Gibbs free energy. The mole fractions of the exsolved and dissolved water and carbon dioxide were calculated on the basis of two chemical equilibrium and two mass-balance equations expressing the conservation of the exsolved and total mass of volatiles. The model was then calibrated by processing nearly 1000 experimental H 2 O and CO 2 solubility determinations from the literature in natural and synthetic silicate liquids covering a wide range of pressure-temperature-composition conditions. The results show that the present model predicts with reasonable accuracy the solubility of water and carbon dioxide in ...
Time-saving video on equilibrium systems. Conjugate acid and base equilibrium systems are a type of chemical equilibrium in which the rate of the forward and reverse acid-base reaction are equal.
Necrotic cells are known to develop characteristic membrane blebs. We measured the protein concentration within necrotic blebs and found that it can be reduced by as much as twenty-fold compared to the main cell body (CB). These results raise two questions: 1. Why do proteins vacate the bleb? and 2. How can osmotic equilibrium be maintained between the bleb and CB? Our photobleaching and ultracentrifugation experiments indicate extensive protein aggregation. We hypothesize that protein aggregation within the CB shifts the chemical equilibrium and draws proteins out of the bleb; at the same time, aggregation reduces the effective molar concentration of protein in the CB, so that osmotic equilibrium between high-protein CB and low-protein necrotic blebs becomes possible.
Chapter Two LeChatlelier -- I thought that the earths climate was in a stable equilibrium -- like a chemical equilibrium. I am a chemist, and it was natural to see the world that way. In chemistry there is a concept called Le Chateliers principle which holds that a chemical system will alter itself to re-establish equilibrium. Last year, I heard a climate change denier make such an argument . (Here is a more sophisticated version.) At the time I thought the same, I thought more CO2 in the air, would mean faster growing forests and more algae in the sea. The biosphere would moderate the increase. As Time passed scientists tested one source of CO2 absorption after another, and nothing is fast enough. There are still people fertilizing the sea to increase CO2 absorption, but the best data shows the algae are eaten by other animals before they sink to the bottom of sea and leave the biosphere. Lets remember ocean fertilization though, because it is a Geoengineering Idea ...
A continuation of either CHE 129 or 131, introducing the fundamental principles of chemistry, including substantial illustrative material drawn from the chemistry of inorganic, organic, and biochemical systems. The principal topics covered are stoichiometry, the states of matter, chemical equilibrium and introductory thermodynamics, electrochemistry, chemical kinetics, electron structure and chemical bonding, and chemical periodicity. The sequence emphasizes basic concepts, problem solving, and factual material. It provides the necessary foundation for students who wish to pursue further coursework in chemistry. This sequence is inappropriate for students who have completed two or more years of chemistry in high school; such students should take CHE 141, 142. Three lecture hours and one 80-minute workshop per week. May not be taken for credit in addition to CHE 142.. Prerequisite: C or higher in CHE 129 or CHE 131; or C or higher in CHE 125 and D or higher in CHE 129 or CHE 131. Pre- or ...
Course concepts include: thermochemical changes; electrochemical changes; chemical equilibrium focusing on acid-base systems; and chemical reactions of select classes of organic compounds. Energy changes and safety are emphasized ...
Order monoclonal and polyclonal CDC42BPB antibodies for many applications. Selected quality suppliers for anti-CDC42BPB antibodies.
Good news about Don Martin, Mad is releasing a deluxe two volume set of Don Martins work. It is being released October 23 and according to the info I have it will contain all of the work Martin created for Mad over the course of 30 years. Both volumes will be over 1000 pages of material so for all of us Don Martin fans it is something to look forward to. Thank you Greg ...
... bromophenol blue), i get a nice result last time (as shown in normal.png) but after i have prepared a new solution of ... the only thing left for me to suggest is for you to run the bromphenol blue to the end of the gel (or off the gel, we used to ... Somemore, during the destasining process, 1cm broad line apart from the indicator blue line is easily washed off and become ... bromophenol blue), i get a nice result last time (as shown in normal.png) but after i have prepared a new solution of ...
Bromphenol Blue , Monograph containing literature references, physical and biological properties and relevant information ...
Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein ... Brom phenol blue, 25 g. Synonyms 3,3,5,5-Tetrabromophenolsulfonphthalein; Bromophenol Blue (BPB); Bromphenol Blue Sultone ... 3,3,5,5-Tetrabromophenolsulfonphthalein; Bromophenol Blue (BPB); Bromphenol Blue Sultone Form ... Bromophenol blue is a tracking dye for alkaline and neutral buffer systems. It is used as a tracking dye in DNA, RNA (agarose) ...
Blue-black powder Notes: Sodium salt Storage Code: Green general chemical storage ... Bromphenol Blue, Reagent Grade, 1 g. Item # 849080 *bvseo_sdk, java_sdk, bvseo-4.0.0 ... Characteristic: Blue-black powder. Notes: Sodium salt. Storage Code: Green general chemical storage ... Characteristic: Blue-black powder. Notes: Sodium salt. Storage Code: Green general chemical storage ...
Antonyms for bromphenol blue. 2 synonyms for bromphenol blue: bromophenol blue, tetrabromo-phenolsulfonephthalein. What are ... Bromphenol blue synonyms, bromphenol blue antonyms - FreeThesaurus.com https://www.freethesaurus.com/bromphenol+blue ... Synonyms for bromphenol blue. a dye used as an acid-base indicator. Synonyms. *bromophenol blue ... bromphenol blue,type:0,children:[{name:bromophenol blue,type:2},{name:tetrabromo-phenolsulfonephthalein,type:2 ...
Use of bromphenol blue printing method for detecting sweat on the palm. M, Sakurai; Kelleher, John C. ...
Bromphenol Blue Indicator, 100ml Mdb Hach. pH indicator for acidity and alkalinity titrations Visual transition interval: pH ... Bromthymol Blue Indicator Powder Pillows Hach. pH indicator Visual transition interval: pH 6.0 (yellow)- pH 7.6 (blue) pk/100 ...
phenolsulfophthalein dyes such as bromphenol blue. salicylates such as sodium salicylate and acetyl salicylate ...
50% glycerol, 0.1M Bromphenol Blue,. 0.01 M Xylene Cyanol, 4X ESA Gel Buffer. ...
... wt/vol bromphenol blue). ApoC-III was isolated by preparative isoelectric focusing gel electrophoresis (8 M urea; 7.5% ... and stained with Coomassie Brilliant Blue R 250 (5). ...
Old school bromphenol blue dye. * dye overnight or cycles of 1 min @ power 6 in the microwave * microwave by Bos bench. Let it ... Bromphenol Blue. Is it important to degas my water + buffer + acrylamide mix before adding the APS and TEMED, as the manuals ... New school GelCode Blue Safe Protein Stain. * Product Info: * GelCode Blue Safe Protein Stain: A coomassie gel stain thats ... note: Coomassie Brilliant Blue G-250 differs from Coomassie Brilliant Blue R-250 by the addition of two methyl groups. We use ...
loading solution, Type I ... polyacrylamide and agarose gel ... and offers the advantage ... (bromphenol blue and xylene ... ... faster than Bromophenol blue,or methylene blue ... dye migrates with DNA between 10 ... this size,prevents the obscuring of ...
... methylene blue, DAPI, acridine orange and others. We offer a wide range of dyes and loading buffers used for nucleic acid ... Bromphenol Blue sodium salt. B5525. Bromphenol Blue-Xylene Cyanole Dye solution. B3269. ... Methylene blue. SYBR® Green I. SYBR® Green II. Pyronin Y. DAPI. Acridine Orange. ... methylene blue, DAPI, acridine orange and others. The table helps identify the stains, which can be used for various nucleic ...
bromphenol blue. More Info. *CAS 115-39-9. *Flinn MSDS. *Wikipedia. Molar Mass. *669.96 g/mol ...
Bromphenol Blue. Sigma. B-0126. NaF. Sigma. S7920. DOWNLOAD MATERIALS LIST. References. *Renart, J., Reiser, J., Stark, G. R. ...
Bromphenol Blue Indicator Solution 100ml £17.63 (exc. VAT) £21.16 (inc. VAT) 1 in range Details ...
... bromphenol blue; 50% [v/v] glycerol). Samples were then subjected to electrophoresis through 26-well 4% to 20% gradient Tris- ...
Methylene Blue (3). 0.02-1.00. 50. R1. 3654-02-SC. Surfactants. Bromphenol Blue (3). 0.5-8.0. 100. LQ. 4876-01. ...
bromphenol blue. EMSA. electrophoretic mobility shift assay. ICB. inverted CCAAT box. MGB. minor groove binder. ... bromphenol blue (BFB). Bio-Rad high range SDS-polyacrylamide gel electrophoresis molecular mass standards were used as a ... 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide (thiazolyl blue). bp. base pair(s). DTT. dithiothreitol. BFB. ...
Restriction Fragment Length Polymorphism Paper Towel Nick Translation Bromphenol Blue Restriction Fragment Length Poly These ...
0.1 % (w/v) Bromphenol blue * Solve in ddH20 LB medium. For 1 L of LB medium you need: * 10 g Trypton ...
bromphenol blue, ponceau S, Coomassie brillant blue 250. What test measures the dye absorption by spectrophotometry as a shift ...
... bromphenol blue in 0.125 M Tris HCl). Protein from each sample and 10??l ProSieve? molecular weight marker (Cambrex Bioscience ... Cytosolic DHE exhibits blue fluorescence; however, once this probe is oxidized by O2- to 2-hyroethidium (2-HE) and ethidium it ...
... bromphenol blue). For immunoblotting, samples were separated on 10% SDS polyacrylamide gels and transferred onto nitrocellulose ... Cell viability was assessed by the trypan blue dye exclusion method. Briefly, cells were seeded into two 3.5 mm dishes, the ... After 48 hours cells were trypsinized and diluted 1:1 in 0.4% sterile filtered trypan blue solution. The cells were incubated ... Viable cells appeared bright, dead cells were blue. Three independent experiments were performed, each time both sides of the ...
... bromphenol blue; 20% glycerol; 100 mM dithiothreitol). The extracts were separated on an SDS-polyacrylamide gel electrophoresis ... Blue color indicates interaction. The quantitative determination of theβ -galactosidase activity is given in arbitrary units. ... Interaction was determined qualitatively based on blue coloration of colonies overlaid with X-Gal. This demonstrated an ...
... and a trace of bromphenol blue. 2D IEF SDS-PAGE was performed for the three infections and at four timepoints of harvesting by ... Proteins responsible for protein-protein interaction (14-3-3 like protein), ion binding (blue type copper domain) and NAD ... blue (type 1) copper protein (29 fold 5 h) involved in signal transduction as well as v-H(+)-ATPase subunit A (39 fold 5 h) ... Coomassie Brilliant Blue (BioRad) overnight and scanned on a UMAX Power Look III Scanner (UMAX Technologies, Fremont, CA, U.S.A ...
... research Bromophenol Blue Sodium Salt Solution manufacturer. Just in time delivery in U.S. Price. Free samples program. Term ... About Bromophenol Blue Sodium Salt Solution. Bromphenol Blue Solution is generally immediately available in most volumes. High ... Bromophenol Blue Sodium Salt Solution Synonyms. Bromophenol Blue sodium salt; Blue Bromophenol Sodium Salt; Sodium bromophenol ... Bromophenol Blue Sodium Salt Solution Properties (Theoretical). Compound Formula. C19H9Br4O5SNa. ...
Available bilirubin binding sites of serum from newborns determined by a direct spectrometric method using bromphenol blue. ...
Before gel electrophoresis, 1 μl of 5% bromphenol blue solution was added to each sample. ...
Data in this table are for sodium salts of thymol blue, bromphenol blue, tetrabromphenol blue, bromcresol green, methyl red, ... Bromphenol blue. 6.2-7.6. 1 drop 0.1% aq. soln.. yellow. blue. p-Nitrophenol. 5.0-7.0. 1-5 drops 0.1% aq. soln.. colorless. ... Bromphenol blue. 3.0-4.6. 1 drop 0.1% aq. soln.. yellow. blue-violet. ... Tried-and-true indicators include: thymol blue, tropeolin OO, methyl yellow, methyl orange, bromphenol blue, bromcresol green, ...
Methylene Blue (3). 50. LAM3654SC. Surfactants. 0.00-8.00. 0.75. Bromphenol Blue (3) ...
  • Each PCR product for [Zn.sup.2+]-cyclen-PAGE was dissolved in a half amount of a loading dye containing 50 mmol/L EDTA, 0.5 g/L bromphenol blue , and 300 mL/L glycerol and then applied (0.5-1.0 ng of DNA per well). (freethesaurus.com)
  • For SDS-PAGE, the samples were centrifuged (20 000g for 5 min) and denatured by heating at 95[degrees]C for 3 min in a modified gel-loading buffer containing 15 g/L glycine, 3.5 g/L Tris-HCl (pH 6.8),1 g/L SDS, 100 mL/L glycerol, and 1 g/L bromphenol blue but no [beta]-mercaptoethanol. (freethesaurus.com)
  • After incubation at 45°C for 15 minutes, the reaction is terminated by addition of stop dye (50% glycerol, 50 mM EDTA and bromophenol blue), heated at 70°C for 10 minutes and then loaded on a 0.7% agarose gel. (neb.com)
  • sample loading buffer should be just bromphenol blue in glycerol. (biotechniques.com)
  • 2X SDS-PAGE Sample Buffer consists of 0.125 M Tris-HCl, 4% (w/v) SDS, 20% (v/v) Glycerol and 0.01% (w/v) bromphenol blue. (rockland-inc.com)
  • Bromophenol blue migrates at approximately the same rate as 300-500bp DNA in agarose gel and at the buffer front in protein polyacrylamide gels. (mpbio.com)
  • Bromophenol blue is a tracking dye for alkaline and neutral buffer systems. (mpbio.com)
  • Reagent grade chemicals shall be used in all tests and includes the following: water, bromphenol blue indicator solution, bromcresol green indicator solution, chloroform, hydrochloric acid standard solution, isopropyl alcohol, phenyl isothiocyanate, and salicylaldehyde. (astm.org)
  • Equal volumes of sample and loading buffer, consisting of 4 M urea with bromphenol blue , were mixed, and 10 [micro]L was loaded on the gel. (freethesaurus.com)
  • The protein is suspended in SDS/mercaptoethanol sample buffer with a bromphenol blue dye marker. (abcam.com)
  • 10), the apical cytoplasm was filled with granules that were eosinophilic and did not stain with PAS, bromphenol blue , or WGA. (freethesaurus.com)
  • Dipstick urinalysis detects protein with Bromphenol blue indicator dye and is most sensitive to albumin and less sensitive to Bence-Jones protein and globulins. (amazonaws.com)
  • A wide range of nucleic acid stains for polyacrylamide and agarose are available including Nancy-520, Ethidium bromide, SYBR ® Green I and II, methylene blue, DAPI, acridine orange and others. (sigmaaldrich.com)
  • Anionic surfactants are extracted with toluene and break up an ion pair, releasing bromphenol blue into a water layer. (ultimatewasher.com)
  • Add 0.04 g of bromphenol blue in 75 ml of DI water, then dilute to a final volume of 100 ml with DI water. (boreal.com)
  • We also validated the method by assaying several serum samples with known autoantibody profiles in separate wells, and we added bromphenol blue to the spotting solutions to monitor for any failure in probe deposition. (freethesaurus.com)