Dosage forms of a drug that act over a period of time by controlled-release processes or technology.
Compounds formed by the joining of smaller, usually repeating, units linked by covalent bonds. These compounds often form large macromolecules (e.g., BIOPOLYMERS; PLASTICS).
Elements of limited time intervals, contributing to particular results or situations.
Forms to which substances are incorporated to improve the delivery and the effectiveness of drugs. Drug carriers are used in drug-delivery systems such as the controlled-release technology to prolong in vivo drug actions, decrease drug metabolism, and reduce drug toxicity. Carriers are also used in designs to increase the effectiveness of drug delivery to the target sites of pharmacological actions. Liposomes, albumin microspheres, soluble synthetic polymers, DNA complexes, protein-drug conjugates, and carrier erythrocytes among others have been employed as biodegradable drug carriers.
Chemistry dealing with the composition and preparation of agents having PHARMACOLOGIC ACTIONS or diagnostic use.
A disorder caused by hemizygous microdeletion of about 28 genes on chromosome 7q11.23, including the ELASTIN gene. Clinical manifestations include SUPRAVALVULAR AORTIC STENOSIS; MENTAL RETARDATION; elfin facies; impaired visuospatial constructive abilities; and transient HYPERCALCEMIA in infancy. The condition affects both sexes, with onset at birth or in early infancy.
The preparation, mixing, and assembling of a drug. (From Remington, The Science and Practice of Pharmacy, 19th ed, p1814)
A water-soluble, enzyme co-factor present in minute amounts in every living cell. It occurs mainly bound to proteins or polypeptides and is abundant in liver, kidney, pancreas, yeast, and milk.
A nucleoside that substitutes for thymidine in DNA and thus acts as an antimetabolite. It causes breaks in chromosomes and has been proposed as an antiviral and antineoplastic agent. It has been given orphan drug status for use in the treatment of primary brain tumors.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
Antibodies produced by a single clone of cells.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Immunoglobulins produced in response to VIRAL ANTIGENS.
A CELL CYCLE and tumor growth marker which can be readily detected using IMMUNOCYTOCHEMISTRY methods. Ki-67 is a nuclear antigen present only in the nuclei of cycling cells.
A syndrome characterized by chronic, well-established DIARRHEA (greater than one month in duration) without an identified infectious cause after thorough evaluation, in an HIV-positive individual. It is thought to be due to direct or indirect effects of HIV on the enteric mucosa. HIV enteropathy is a diagnosis of exclusion and can be made only after other forms of diarrheal illness have been ruled out. (Harrison's Principles of Internal Medicine, 13th ed, pp1607-8; Haubrich et al., Bockus Gastroenterology, 5th ed, p1155)
An optical source that emits photons in a coherent beam. Light Amplification by Stimulated Emission of Radiation (LASER) is brought about using devices that transform light of varying frequencies into a single intense, nearly nondivergent beam of monochromatic radiation. Lasers operate in the infrared, visible, ultraviolet, or X-ray regions of the spectrum.
Drugs that are pharmacologically inactive but when exposed to ultraviolet radiation or sunlight are converted to their active metabolite to produce a beneficial reaction affecting the diseased tissue. These compounds can be administered topically or systemically and have been used therapeutically to treat psoriasis and various types of neoplasms.
Products or parts of products used to detect, manipulate, or analyze light, such as LENSES, refractors, mirrors, filters, prisms, and OPTICAL FIBERS.
Therapy using oral or topical photosensitizing agents with subsequent exposure to light.
The process of aging due to changes in the structure and elasticity of the skin over time. It may be a part of physiological aging or it may be due to the effects of ultraviolet radiation, usually through exposure to sunlight.
A purine or pyrimidine base bonded to DEOXYRIBOSE.
Nucleosides in which the purine or pyrimidine base is combined with ribose. (Dorland, 28th ed)
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
Purine or pyrimidine bases attached to a ribose or deoxyribose. (From King & Stansfield, A Dictionary of Genetics, 4th ed)
A musculomembranous sac along the URINARY TRACT. URINE flows from the KIDNEYS into the bladder via the ureters (URETER), and is held there until URINATION.
Implanted fluid propulsion systems with self-contained power source for providing long-term controlled-rate delivery of drugs such as chemotherapeutic agents or analgesics. Delivery rate may be externally controlled or osmotically or peristatically controlled with the aid of transcutaneous monitoring.
The level of governmental organization and function at the national or country-wide level.
Includes the spectrum of human immunodeficiency virus infections that range from asymptomatic seropositivity, thru AIDS-related complex (ARC), to acquired immunodeficiency syndrome (AIDS).
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
The number of WHITE BLOOD CELLS per unit volume in venous BLOOD. A differential leukocyte count measures the relative numbers of the different types of white cells.
The complex of political institutions, laws, and customs through which the function of governing is carried out in a specific political unit.
Agents used to treat AIDS and/or stop the spread of the HIV infection. These do not include drugs used to treat symptoms or opportunistic infections associated with AIDS.
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
A computer based method of simulating or analyzing the behavior of structures or components.
A post-MORULA preimplantation mammalian embryo that develops from a 32-cell stage into a fluid-filled hollow ball of over a hundred cells. A blastocyst has two distinctive tissues. The outer layer of trophoblasts gives rise to extra-embryonic tissues. The inner cell mass gives rise to the embryonic disc and eventual embryo proper.
A discipline concerned with studying biological phenomena in terms of the chemical and physical interactions of molecules.
Measure of the number of the PARASITES present in a host organism.
A pyrimidine nucleoside analog that is used mainly in the treatment of leukemia, especially acute non-lymphoblastic leukemia. Cytarabine is an antimetabolite antineoplastic agent that inhibits the synthesis of DNA. Its actions are specific for the S phase of the cell cycle. It also has antiviral and immunosuppressant properties. (From Martindale, The Extra Pharmacopoeia, 30th ed, p472)
An alkaloid isolated from Colchicum autumnale L. and used as an antineoplastic.
The guidelines and policy statements set forth by the editor(s) or editorial board of a publication.
A major protein in the BLOOD. It is important in maintaining the colloidal osmotic pressure and transporting large organic molecules.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Tumors or cancer of the RECTUM.
Nuclear antigen with a role in DNA synthesis, DNA repair, and cell cycle progression. PCNA is required for the coordinated synthesis of both leading and lagging strands at the replication fork during DNA replication. PCNA expression correlates with the proliferation activity of several malignant and non-malignant cell types.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Evaluation undertaken to assess the results or consequences of management and procedures used in combating disease in order to determine the efficacy, effectiveness, safety, and practicability of these interventions in individual cases or series.

The incorporation of 5-iodo-2'-deoxyuridine into the DNA of HeLa cells and the induction of alkaline phosphatase activity. (1/3497)

Inhibition of DNA synthesis during the period of exposure of HeLa cells to 5-iodo-2'-deoxyuridine (IUdR) inhibited the induction of alkaline phosphatase activity. This finding, taken together with previous findings that IUdR did not induce alkaline phosphatase activity in the presence of 2-fold molar excess thymidinemonstrated that IUdR incorporation into DNA is correlated with the increase in alkaline phosphatase activity. With the exception of an interim period described in the text, induction of alkaline phosphatase activity was linearly related to medium concentrations of IUdR of up to at least 3 muM. However, the extent of IUdR substitution in DNA did not appear to be related to the degree of enzyme induction. Alkaline phosphatase activity continued to increase at medium concentrations of IUdR from 1 to 3 muM, while little further substitution of DNA occurred.  (+info)

High-throughput screening of small molecules in miniaturized mammalian cell-based assays involving post-translational modifications. (2/3497)

BACKGROUND: Fully adapting a forward genetic approach to mammalian systems requires efficient methods to alter systematically gene products without prior knowledge of gene sequences, while allowing for the subsequent characterization of these alterations. Ideally, these methods would also allow function to be altered in a temporally controlled manner. RESULTS: We report the development of a miniaturized cell-based assay format that enables a genetic-like approach to understanding cellular pathways in mammalian systems using small molecules, rather than mutations, as the source of gene-product alterations. This whole-cell immunodetection assay can sensitively detect changes in specific cellular macromolecules in high-density arrays of mammalian cells. Furthermore, it is compatible with screening large numbers of small molecules in nanoliter to microliter culture volumes. We refer to this assay format as a 'cytoblot', and demonstrate the use of cytoblotting to monitor biosynthetic processes such as DNA synthesis, and post-translational processes such as acetylation and phosphorylation. Finally, we demonstrate the applicability of these assays to natural-product screening through the identification of marine sponge extracts exhibiting genotype-specific inhibition of 5-bromodeoxyuridine incorporation and suppression of the anti-proliferative effect of rapamycin. CONCLUSIONS: We show that cytoblots can be used for high-throughput screening of small molecules in cell-based assays. Together with small-molecule libraries, the cytoblot assay can be used to perform chemical genetic screens analogous to those used in classical genetics and thus should be applicable to understanding a wide variety of cellular processes, especially those involving post-transitional modifications.  (+info)

Herpetic keratitis. Proctor Lecture. (3/3497)

Although much needs to be learned about the serious clinical problem of herpes infection of the cornea, we have come a long way. We now have effective topical antiviral drugs. We have animal models which, with a high degree of reliability, clearly predict the effect to be expected clinically in man, as well as the toxicity. We have systemically active drugs and the potential of getting highly active, potent, completely selective drugs, with the possibility that perhaps the source of viral reinfection can be eradicated. The biology of recurrent herpes and stromal disease is gradually being understood, and this understanding may result in new and better therapy of this devastating clinical disease.  (+info)

Immunochemical detection and isolation of DNA from metabolically active bacteria. (4/3497)

Most techniques used to assay the growth of microbes in natural communities provide no information on the relationship between microbial productivity and community structure. To identify actively growing bacteria, we adapted a technique from immunocytochemistry to detect and selectively isolate DNA from bacteria incorporating bromodeoxyuridine (BrdU), a thymidine analog. In addition, we developed an immunocytochemical protocol to visualize BrdU-labeled microbial cells. Cultured bacteria and natural populations of aquatic bacterioplankton were pulse-labeled with exogenously supplied BrdU. Incorporation of BrdU into microbial DNA was demonstrated in DNA dot blots probed with anti-BrdU monoclonal antibodies and either peroxidase- or Texas red-conjugated secondary antibodies. BrdU-containing DNA was physically separated from unlabeled DNA by using antibody-coated paramagnetic beads, and the identities of bacteria contributing to both purified, BrdU-containing fractions and unfractionated, starting-material DNAs were determined by length heterogeneity PCR (LH-PCR) analysis. BrdU-containing DNA purified from a mixture of DNAs from labeled and unlabeled cultures showed >90-fold enrichment for the labeled bacterial taxon. The LH-PCR profile for BrdU-containing DNA from a labeled, natural microbial community differed from the profile for the community as a whole, demonstrating that BrdU was incorporated by a taxonomic subset of the community. Immunocytochemical detection of cells with BrdU-labeled DNA was accomplished by in situ probing with anti-BrdU monoclonal antibodies and Texas red-labeled secondary antibodies. Using this suite of techniques, microbial cells incorporating BrdU into their newly synthesized DNA can be quantified and the identities of these actively growing cells can be compared to the composition of the microbial community as a whole. Since not all strains tested could incorporate BrdU, these methods may be most useful when used to gain an understanding of the activities of specific species in the context of their microbial community.  (+info)

Proteolipid protein gene product can be secreted and exhibit biological activity during early development. (5/3497)

A gene encoding myelin proteolipid protein (PLP) and its smaller isoform DM20 is expressed at least 1 week before myelination. Mutations within the gene cause abnormalities in the development of premyelinating oligodendrocytes, resulting in hypomyelinating disorders. These findings suggest a premyelinating function of the PLP gene products. We previously demonstrated that PLP gene expression is directly associated with secretion of a factor that increases the number of oligodendrocytes. Here we show that this activity is mediated by a secreted fragment containing the C-terminal portion of PLP. This factor increased the bromodeoxyuridine incorporation rate in both oligodendrocyte and astrocyte lineage cells; a synthetic peptide (PLP 215-232) exhibited a similar activity. Dose-response curves of PLP and PLP peptide showed maximum activities at a concentration in the picomolar range, which decreased at higher concentrations. These observations demonstrate that a secreted PLP gene product exerts biological activity at a premyelinating stage before the major induction of the gene.  (+info)

Embryonic and postnatal injections of bromodeoxyuridine produce age-dependent morphological and behavioral abnormalities. (6/3497)

The mitotic marker 5-bromodeoxyuridine (BrdU) was injected twice daily (60 mg/kg) into pregnant hooded rats on one of embryonic days (E) 11, 12, 13, 15, 17, or 21, or into rat pups on postnatal day (P) 10. The principal findings were the following: (1) BrdU exposure on E11 produces profound effects on body morphology, and animals must be fed a special diet because of chronic tooth abnormalities; (2) BrdU exposure at E17 or earlier produces a change in coat spotting pattern, the precise pattern varying with age; (3) BrdU exposure on E15 or earlier produces a reduction in both brain and body weight; (4) BrdU exposure on E17 or earlier reduces cortical thickness; (5) BrdU exposure on E11-E13 and at P10 reduces cerebellar size relative to cerebral size; (6) spatial learning is significantly affected after injections of BrdU at E11-E17, but the largest effect is on E17; (7) the deficit in spatial learning may be related in part to a reduction in visual acuity; and (8) skilled forelimb ability is most disrupted after BrdU exposure at E15 but is also impaired after injections on E13 or earlier. BrdU thus has teratological effects on body, brain, and behavior that vary with the developmental age of the fetus or infant.  (+info)

Capsaicin-sensitive C-fiber-mediated protective responses in ozone inhalation in rats. (7/3497)

To assess the role of lung sensory C fibers during and after inhalation of 1 part/million ozone for 8 h, we compared breathing pattern responses and epithelial injury-inflammation-repair in rats depleted of C fibers by systemic administration of capsaicin as neonates and in vehicle-treated control animals. Capsaicin-treated rats did not develop ozone-induced rapid, shallow breathing. Capsaicin-treated rats showed more severe necrosis in the nasal cavity and greater inflammation throughout the respiratory tract than did control rats exposed to ozone. Incorporation of 5-bromo-2'-deoxyuridine (a marker of DNA synthesis associated with proliferation) into terminal bronchiolar epithelial cells was not significantly affected by capsaicin treatment in rats exposed to ozone. However, when normalized to the degree of epithelial necrosis present in each rat studied, there was less 5-bromo-2'-deoxyuridine labeling in the terminal bronchioles of capsaicin-treated rats. These observations suggest that the ozone-induced release of neuropeptides does not measurably contribute to airway inflammation but may play a role in modulating basal and reparative airway epithelial cell proliferation.  (+info)

Retinal neurogenesis: the formation of the initial central patch of postmitotic cells. (8/3497)

We have investigated the relationship between the birthdate and the onset of differentiation of neurons in the embryonic zebrafish neural retina. Birthdates were established by a single injection of bromodeoxyuridine into embryos of closely spaced ages. Differentiation was revealed in the same embryos with a neuron-specific antibody, zn12. The first bromodeoxyuridine-negative (postmitotic) cells occupied the ganglion cell layer of ventronasal retina, where they formed a small cluster of 10 cells or less that included the first zn12-positive cells (neurons). New cells were recruited to both populations (bromodeoxyuridine-negative and zn12-positive) along the same front, similar to the unfolding of a fan, to produce a circular central patch of hundreds of cells in the ganglion cell layer about 9 h later. Thus the formation of this central patch, previously considered as the start of retinal neurogenesis, was actually a secondary event, with a developmental history of its own. The first neurons outside the ganglion cell layer also appeared in ventronasal retina, indicating that the ventronasal region was the site of initiation of all retinal neurogenesis. Within a column (a small cluster of neuroepithelial cells), postmitotic cells appeared first in the ganglion cell layer, then the inner nuclear layer, and then the outer nuclear layer, so cell birthday and cell fate were correlated within a column. The terminal mitoses occurred in three bursts separated by two 10-h intervals during which proliferation continued without terminal mitoses.  (+info)

Detect BrdU also known as Bromodeoxyuridine with Anti-BrdU Antibody, clone BU-1 (Mouse Monoclonal Antibody), that has been demonstrated to work in ICC. Find MSDS or SDS, a COA, data sheets and more information.
PromoCells Cell Proliferation Assay Kit II (BrdU) detects incorporated BrdU using a mouse anti-BrdU antibody. An anti-mouse HRP-linked secondary antibody is used to detect the anti-BrdU antibody bound to BrdU, which is followed by addition of TMB (a HRP substrate). The extent of color development is proportional to the quantity of BrdU incorporated into the cells and can be used directly as an indicator of cell proliferation. Compared to other cell proliferation assays, this kit detects only the proliferating cells and not the seeded cells. This highly sensitive, non-radioactive kit detects as less as 50-100 proliferating cells. ...
Chinese hamster ovary cells were seeded in the absence or presence of the polyamine synthesis inhibitor 2-difluoromethylornithine (DFMO). At 1-4 days after seeding, the cells were labelled for 15-120 min with the thymidine analogue bromodeoxyuridine (BrdUrd) and they were then fixed directly after the labelling period. In addition, cells were labelled for 30 min and they were then allowed to progress in BrdUrd-free medium during a defined post-labelling time before fixation. An indirect immunofluorescence technique, using the monoclonal BrdUrd antibody and the intercalating stochiometric DNA stain, propidium iodide, was applied to enable quantification of cellular BrdUrd and DNA contents, respectively, by flow cytometry (FCM). By comparing the mean DNA content of BrdUrd-labelled cells to the mean DNA contents of G(1) and G(2) cells, a relative measure of the position of the BrdUrd-labelled cells was obtained (relative movement). Relative movement data, obtained from control and DFMO-treated ...
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DiviTum™ assay determines the enzymatic activity of TK in patient samples. During the assay procedure, thymidine is replaced by its synthetic analog bromodeoxyuridine (BrdU), which gets phosphorylated and then incorporated into a synthetic DNA strand fixated in each well of a 96-well ELISA immunosorbent titer-plate.. The extent of BrdU incorporation depends on the activity of TK present in the serum sample; the more TK activity in the sample, the more BrdU is incorporated into synthetic DNA strands in the titer-plate well. The synthetic BrdU is then detected with anti-BrdU specific antibodies using the well-known ELISA assay technique. The DiviTum™ technology amplifies the signal, enabling the assay to measure thymidine kinase activity with high sensitivity.. ...
Abstract The interaction of a nucleoside analogue bromodeoxyuridine (BrdU) with human serum albumin (HSA) was studied to investigate the binding phenomenon and analyse the protein conformation upon...
Mouse anti-BrdU antibody, clone Bu20a recognizes incorporated BrdU in a variety of cell types. It is suitable for use on tissue sections in double-labeling techniques.
Anti-BrdU monoclonal antibody is offered as a stand alone product, for use in labeling cells that have incorporated BrdU (cells incubated in the presence of BrdU will incorporate this thymidine analog into any DNA that is synthesized during that time). This antibody, from the PRB-1 clone ... ...
Monoclonal antibody against 5-bromo-2-deoxyuridine BrdU expressed by BrdU for use in FACS, FFPE, Immunofluorescence, Immunohistochemistry against All species labeled with BrdU
We have brought together our full range of anti-BrdU antibodies in one place, making it easy for you to find exactly what you need. Buy online today
TY - JOUR. T1 - Increased incorporation of 5-bromodeoxyuridine into the DNA of proliferating tissues in partially hepatectomised mice. AU - Hill, Bridget T.. AU - Augenlicht, Leonard H.. AU - Baserga, Renato. PY - 1973/12/1. Y1 - 1973/12/1. UR - http://www.scopus.com/inward/record.url?scp=0015897107&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0015897107&partnerID=8YFLogxK. U2 - 10.1016/0014-5793(73)80482-X. DO - 10.1016/0014-5793(73)80482-X. M3 - Article. C2 - 4763337. AN - SCOPUS:0015897107. VL - 37. SP - 298. EP - 302. JO - FEBS Letters. JF - FEBS Letters. SN - 0014-5793. IS - 2. ER - ...
Return to Modified Bases Modifications 5-Bromo-deoxyuridine is a photoreactive halogenated base that can be incorporated into oligonucleotides to crosslink them to DNA, RNA or proteins with exposure to UV light. Crosslinking is maximally efficient with light at 308 nm.. ...
Mouse genotyping. Null and wild-type mice were obtained from heterozygous p27Kip1 breeding pairs (in a mixed genetic background of C57BL/6 and B6SJL) (Kiyokawa et al., 1996). The genotype was determined by PCR analysis of genomic tail DNA. Briefly, tails were digested in lysis buffer (100 mm NaCl, 10 mm Tris, pH 8.0, 0.5% SDS, 25 mm EDTA, 148 μg/ml proteinase K stock) for 14-18 hr at 55°C. Genomic DNA was isolated and amplified by PCR using the following primers: 5′-CGCCCCGACTGCATCTGCGTGTTCGAA-3′ and 5′-TCAAACGTGAGAGTGTCTAACGG-3′ directed against thep27Kip1 gene and 5′-AGGGCTTATGATTCTGAAAGTCG-3′ corresponding to the neo sequence. After a 35 cycle reaction (denaturation at 94°C for 45 sec, annealing at 62°C for 1 min, extension at 72°C for 1 min), the wild-type allele yielded a 210 bp PCR product and the null allele yielded a longer 290 bp product because of neo insertion in the first exon.. Whole-mount bromodeoxyuridine incorporation.Bromodeoxyuridine (BrdU, Sigma, St. Louis, ...
Buy our Pescadillo 293T transfected lysate (positive control). ab94282 has been validated in western blot. Abcam now offers a 12-month guarantee.
The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details ...
Mouse Monoclonal Anti-Bromodeoxyuridine/BrdU Antibody (BU20a) [DyLight 488]. Proliferation Marker. Validated: Flow, ICC/IF, IHC-Fr, IHC-P. Tested Reactivity: All Species. 100% Guaranteed.
Mouse Monoclonal Anti-Bromodeoxyuridine/BrdU Antibody (BU20a) [DyLight 550]. Proliferation Marker. Validated: Flow, ICC/IF, IHC-Fr, IHC-P. Tested Reactivity: All Species. 100% Guaranteed.
Analysis of turnover of Treg by BrdU incorporation. BALB/c mice were treated with BrdU administered continuously for 7 d using osmotic pumps. Then, peripheral L
Aldrich-363847; 2-Bromo-4-fluorotoluene 0.98; CAS Number: 1422-53-3; Linear Formula: CH3C6H3(Br)F; find related products, papers, technical documents, MSDS & more at Sigma-Aldrich.
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hey its me ur hot food Not a bad idea, I recall our mouths can take exposure to much higher temperatures I grip it with my teeth and le...
Under normal conditions, the adult zebrafish telencephalic VZ continuously supplies new neurons to the OB (Byrd and Brunjes, 2001; Grandel et al., 2006; Adolf et al., 2006; Kishimoto et al., 2011). We next performed a BrdU pulse-chase experiment to trace the migrating newly born progeny of ventricular proliferative cells from the telencephalic VZ to the injury site (Zupanc et al., 2005; Grandel et al., 2006; Adolf et al., 2006; Kishimoto et al., 2011). We followed the BrdU-labeled cells in three telencephalic regions - the VZ, the subpallium and the mdlPa (the region surrounding the injury site) - on 0, 3, 7, 10 and 14 dpl (Fig. 4A). At 0 dpl, a large number of BrdU-labeled cells was detected in the telencephalic VZ (n=5; Fig. 4B,B′,F). At 3 dpl, BrdU-labeled cells appeared in the subpallium and pallium along the pathway connecting the telencephalic VZ and the injury site in the injured hemisphere (n=5; Fig. 4C,C′,G). From 3 dpl onwards, the number of BrdU-labeled cells decreased in the ...
Under normal conditions, the adult zebrafish telencephalic VZ continuously supplies new neurons to the OB (Byrd and Brunjes, 2001; Grandel et al., 2006; Adolf et al., 2006; Kishimoto et al., 2011). We next performed a BrdU pulse-chase experiment to trace the migrating newly born progeny of ventricular proliferative cells from the telencephalic VZ to the injury site (Zupanc et al., 2005; Grandel et al., 2006; Adolf et al., 2006; Kishimoto et al., 2011). We followed the BrdU-labeled cells in three telencephalic regions - the VZ, the subpallium and the mdlPa (the region surrounding the injury site) - on 0, 3, 7, 10 and 14 dpl (Fig. 4A). At 0 dpl, a large number of BrdU-labeled cells was detected in the telencephalic VZ (n=5; Fig. 4B,B′,F). At 3 dpl, BrdU-labeled cells appeared in the subpallium and pallium along the pathway connecting the telencephalic VZ and the injury site in the injured hemisphere (n=5; Fig. 4C,C′,G). From 3 dpl onwards, the number of BrdU-labeled cells decreased in the ...
In the present study, we examined AQP4 function in reactive astrocytes compared between wild-type (WT) and AQP4-deficient (AQP4/KO) mice after a stab wound to the cerebral cortex. To examine activity of astrocytes, proliferating cells were labeled with 5-bromo-2-deoxyuridine (BrdU) incorporated in the drinking water provided to mice. By the immunofluorescent analysis using anti-BrdU antibody, astrocyte reactivity was at high level around the lesion site for WT mice 3 days after the stab wound to the brain, while it was much less for AQP4/KO mice. To identify the molecules related in injured mouse brain, we performed microarray analysis and found that more than 400 genes around the lesion site were upregulated 3 days after the wounding in WT mice ...
国内在庫あります!Biotin標識済みシープ・ポリクローナル抗体 ab2284 適用: IP,ELISA,IHC-FoFr,IHC-P,IHC-Fr,ICC/IF…BrdU抗体一覧 一次抗体にBiotinを直接標識し、操作時間の短縮と低いバックグラウンドを実現。
Bromodeoxyuridine (BrdU) (Proliferation Marker) Antibody - With BSA and Azide, Mouse Monoclonal Antibody [Clone BRD469 + BRD494 + BRD.3 ] validated in IHC-P, IF, FC (AH10977-20), Abgent
Mice. Lrig1-KO mice were regenerated from cryopreserved sperm (C57BL/6 background) (13). Genotyping was performed using 3 different primers (Supplemental Table 2; WT: 322 bp, KO: 400 bp). The K5 Stat3 Tg mice were as described previously (26). Mice were born with the expected ratio of Mendelian inheritance, and no changes in sex ratios were observed. Tissues. All human cornea tissues were obtained from SightLife Eye Bank, and all corneas were stored at 4°C in storage medium (Optisol-GS; Bausch & Lomb).. Antibodies. For immunohistochemistry, the following antibodies were used: mouse mAb anti-β-catenin (610153, ×200; BD Biosciences), rat mAbs anti-BrdU (ab6326, ×200; Abcam Plc), anti-CD31 (550274, ×200; BD Biosciences), anti-F4/80 (RM2900, ×100; Life Technologies), anti-GR1 (553128, ×100; BD Biosciences), and anti-CD3 (MAB4841, ×100; R&D Systems, Inc.), rabbit polyclonal antibodies (pAbs) anti-LRIG1 (×200; provided by S. Itami, Osaka University), anti-loricrin (PRB-145P, ×700; COVANCE), ...
Now i have a new problem! This BrdU protocol needs Denaturation. Now i want to monitor GFP-transfected cells too. The HCl seems to denature the endogeneous GFP too. Is there a more acid resistable GFP? Perhaps an anti-GFP antibody? Or may i choose another method to denaturize the DNA for the anti-BrdU? Maybe temp.?Or stain for myc-tagged GFP ...
Monoclonal Anti-Bromodeoxyuridine (Clone BU6-4) is often used in cell cycle analysis studies; the level of bromodeoxyuridine (BrdU) incorporation into DNA is a direct parameter of cell number and growth. The monoclonal anti-bromodeoxyuridine antibody can be used in Western blot analysis under non-reducing and non-heating conditions and in flow cytometry applications. ...
CAS NO:656-65-5; Chemical name:4-Bromo-3-fluoroaniline ; physical and chemical property of 656-65-5, 4-Bromo-3-fluoroaniline is provided by ChemNet.com
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Because our examination of cell death did not reveal a loss of newly generated neurons in the cortical plate, we asked whether the reduced neuronal numbers in p107-deficient mice was caused by fewer neurons born at E13.5 (the time of BrdU injection). To address this question, we performed a 24-h BrdU incorporation to measure the rate of neuronal commitment. Pregnant dams were injected at E13.5, embryos were collected 24 h later at E14.5, and the number of strongly labeled BrdU-positive cells were counted (i.e., cells that underwent terminal mitosis at the time of injection). In addition, sections were double stained with PCNA to show that these cells are no longer cycling. Double labeling with BrdU and PCNA revealed that most BrdU-positive cells within the SVZ and IZ were no longer expressing PCNA, indicating that they were newly postmitotic (Fig. 8, d-f). Cell counts of this newly postmitotic population revealed a two-fold reduction in p107-deficient brains compared with wild-type controls ...
Mice exposed to the OIR model were injected with 0.1 mg/g 5-bromo-2-deoxyuridine or 5-ethynyl-2-deoxyuridine (EdU) dissolved in sterile water 21 and were humanely killed 2 hours after injection. For BrdU, the eyes were fixed in 4% paraformaldehyde (PFA) on ice for 3 minutes, then transferred to 70% ethanol and stored at −20°C for 2 hours. They were then washed in decreasing concentrations of ethanol for 10 minutes each, dissected in PBS, and washed for 30 minutes in 1 mL PBS/1% Triton X-100 on a horizontal shaker at low speed. The retinas were incubated at 37°C for 1 hour with 2 N HCl, washed in 1 mL 0.1 M sodium borate twice for 15 minutes, incubated overnight at 4°C with biotin-conjugated anti-BrdU antibody diluted 1:300 in PBS containing 1% BSA, and then washed three times with PBS-1% Triton X-100 for 5 minutes while being shaken at low speed. Retinas were incubated in Texas Red-labeled anti-biotin diluted 1:500 in PBS, 1% BSA for 2 hours with shaking while shielded from light, then ...
As the previously described method [4,22], 5-bromo-2-deoxyuridine (BrdU) immunohistochemistry was performed. The brain sections were treated with 0.5% Trioton X-100 in PBS for 20 minutes, treated with 50% formamide-2 x standard saline citrate at 65℃ for 2 hours, treated with 2 N HCl at 37℃ for 30 minutes, and then treated with 100mM sodium borate (pH, 8.5). The sections were treated with mouse monoclonal anti-BrdU antibody (1:600; Roche) during overnight at 4℃, treated with biotionylated mouse secondary antibody (1:200; Vector Laboratories, Burlingame, CA, USA) for 90 minutes, and treated with avidin-peroxidase complex (1:100; Vector Laboratories). To visualize, the section was treated with 50mM Tris-HC1 (pH, 7.6) in 0.02% DAB, 40-mg/mL nickel chloride, and 0.03% H2O2 during 5 minutes. With a mouse monoclonal anti-neuronal nucleic antibody (1:300; Chemicon International, Temecula, CA, USA), counter-staining was conducted upon the same sections. After the slides were dried under the room ...
Purpose: To examine the anatomical localization of limbal T cells relative to label-retaining cells (LRCs). Previously, we demonstrated the presence of presumed limbal regulatory (Treg) Foxp3-GFP+ T cells via in vivo epifluorescent imaging. In this study, we further characterize the localization of T cells in the limbus in conjunction with LRCs, i.e. presumed limbal stem cells, via immunofluorescence tomography.. Methods: H2B-GFP/K5tTA (HGK) mice express histone H2B-green fluorescent protein (GFP) under the control of the tetracycline and keratin 5 promoters, resulting in retention of GFP in slow-cycling LRCs after doxycycline administration. Following a 16 week pulse-chase period, the limbal region of HGK mice was fixed with 2% paraformaldehyde, sectioned and embedded in butyl-methyl-methacrylate, subjected to immunohistochemistry (IHC) with the T cell marker CD3, and imaged via immunofluorescence tomography to create high-resolution 3-D reconstructions of 200 sections. 3-D reconstructions were ...
TY - JOUR. T1 - Cell cycle analysis using flow cytometry. AU - Gray, J. W.. AU - Dolbeare, F.. AU - Pallavicini, M. G.. AU - Beisker, W.. AU - Waldman, F.. PY - 1986/1/1. Y1 - 1986/1/1. N2 - This manuscript reviews the utility of flow cytometry for the study of cell proliferation. The applications of univariate DNA distribution analysis to cytokinetic studies of asynchronous and perturbed cell populations are discussed briefly. The newly developed technique for simultaneous flow cytometric measurement of cellular DNA content and amount of incorporated bromodeoxyuridine is discussed in more detail. The cytochemistry required for this analysis is reviewed as are its applications to: (a) determination of the fractions of cells in the G1-, S- and G2 + M phases of the cell cycle; (b) determination of the G1-, S- and G2 + M phase durations and dispersions and growth fraction for asynchronous cells; (c) detection of ara-C resistant cells present at low frequency in an otherwise sensitive population; ...
Overall, our 2H2O labeling results are consistent with measurements of hippocampal neurogenesis by BrdU labeling. Assuming that the hippocampus has ≈2 × 106 total cells (Abusaad et al., 1999), the labeling rate of 0.2% per day in total C57Bl/6 hippocampus that we observed with 2H2O would be equivalent to ≈4000 labeled cells/day, a value comparable with estimates by 12- to 24-h saturation labeling with BrdU (Hayes and Nowakowski, 2002). Palmer et al. (1999) reported that 0.7% of progenitor cells were labeled after daily BrdU injection for 6 days in a confocal analysis of BrdU incorporation into gradient-isolated progenitor cells from rat hippocampus, a similar value as we measured by 2H2O labeling on similarly isolated rat hippocampal progenitors (1% per week). In mice, the hierarchy of baseline progenitor cell proliferation across different mouse strains in our study agreed well with strain effects on total hippocampal cell proliferation obtained by BrdU labeling (C57Bl/6 , BALB/c , ...
One of the major drawbacks of droplet sorting in a flow cytometer is the relatively low sorting speed. Thus, we have developed an alternative, faster sorting technique: photodamage cell sorting. In a photodamage cell sorter all unwanted cells, as detected with the first, measuring laser, are killed with the second, damaging laser. Thus, the cells need to be photosensitive to the second laser. In addition, a mechanism is needed to switch this laser on and off based on the sorting criteria. In our photodamage cell sorter, the ZAPPER, we use an acousto-optic crystal to switch the laser beam. Cells are made photosensitive by vital staining with photosensitizers. With cells grown in the presence of 5-bromo-2′-deoxyuridine (BrdUrd) and stained with Hoechst 33342 (H42) at least a 5-decade cell reduction is accomplished after irradiation with 400 mW UV light. With this system, sorting rates have been achieved of 30,000 cells per second. Due to the selection based on photodynamic killing, this sorting ...
Results: This study reports the eventual outcome of the 97 patients after 12 years. There were no significant associations between proliferation data of the index tumours and patient outcome. No adverse events were identified which could be attributed to the use of the halogenated pyrimidine label in vivo ...
Microfiche of typescript. [Urbana, Ill.]: Photographic Services, University of Illinois, U of I Library, [1989]. 2 microfiches (41 frames): negative ...
We have studied the effect of prenatal (late intrauterine) 5-bromo-2-deoxyuridine (BrdU) administration on the development of the GnRH pathway. BrdU is widely accepted as a tool for studying neurogenesis (pre and postnatal). On the other hand, this thymidine analogue proved to be effective influencing different developmental events too. Dams of C57Bl6 mice were treated on the last days of pregnancy (between E16, E17, E18, E19 and the time of birth, E20/21 days, respectively). The dams received daily injections of 15 ug/g body weight BrdU subcutaneously, dissolved in physiological saline, between 11 and 12 am. BrdU treatment during the last 3-4 days of pregnancy resulted in a significant decrease of the number of GnRH immunoreactive (ir.) neuronal cell bodies as well as ir. fibers. This decrease could be detected at all postnatal ages (P0/P1, P3/4, P6/8 and P21/23) studied. The higher cumulative doses of BrdU produced a more significant decrease of the GnRH ir. structures in comparison with the ...
symbol: BrUra; 5‐bromouracil; 5‐bromo‐2,4(1H,3H)‐pyrimidine‐dione; a synthetic analogue of thymine with mutagenic activity. It is incorporated into DNA as bromodeoxyuridine, which replaces thymidine and induces transitions of G‐C base‐pairing to A‐T ... ...
We report a novel mechanism of cellular growth control. Increasing the density of endothelial or smooth muscle cells in culture increased cell-cell contact and decreased cell spreading, leading to growth arrest. Using a new method to independently control cell-cell contact and cell spreading, we fou …
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Kent. D. Dunlap, Anna C. Silva and Michael Chung. Environmental complexity, seasonality and brain cell proliferation in a weakly electric fish, Brachyhypopomus gauderio. J exp Biol 2011; 214, 794-805 Full Text IH. Pytte CL, Parent C, Wildstein S, Varghese C, Oberlander S. Deafening decreases neuronal incorporation in the zebra finch caudomedial nidopallium (NCM). Behav Brain Res. 2010 Aug 25;211(2):141-7 Full Text IH. Lassmann J, Sliwoski J, Chang A, Canning DA, Zderic SA. Deletion of One SERCA2 Allele Confers Protection Against Bladder Wall Hypertrophy in a Murine Model of Partial Bladder Outlet Obstruction. Am J Physiol Regul Integr Comp Physiol. 2008 Jan;R58-65 Full Text ...
TY - JOUR. T1 - Wound splinting modulates granulation tissue proliferation. AU - Carlson, Mark Alan. AU - Thompson, Jon S. PY - 2004/7/1. Y1 - 2004/7/1. N2 - Attachment of the extracellular matrix to a substratum is important for fibroblast survival and proliferation in three-dimensional in vitro culture systems. We hypothesized that wound matrix attachment in a wound splinting model would modulate wound cell proliferation in vivo. Male rats were excisionally wounded on the dorsum, and a splint was sutured to the wound edge. In one experiment (N=12), 6 rats were desplinted on day 5, and then all were sacrificed 24 h later, 6 h after 5-bromo-2′-deoxyuridine (BrdU) injection. In the second experiment (N=18), 6 rats each were desplinted, desplinted with wound edge release, or not disturbed, followed by BrdU injection and sacrifice 24 h later. BrdU-labeled nuclei were quantified on frozen sections of granulation tissue, cut at three different levels. In the first experiment, the percentage of ...
Subventricular Zone-Derived Neural Progenitor Cells Migrate Along a Blood Vessel Scaffold Toward the Post-Stroke Striatum Kojima T, Hirota Y, Ema M, Takahashi S, Miyoshi I, Okano H, Sawamoto K.. The subventricular zone (SVZ) of the adult brain contains neural stem cells that have the capacity to regenerate new neurons after various insults. Brain ischemia causes damage to brain tissue and induces neural regeneration together with angiogenesis. We previously reported that, after ischemic injury in mice, SVZ-derived neural progenitor cells (NPCs) migrate into the striatum, and these NPCs are frequently associated with blood vessels in the regenerating brain tissue. Here we studied the role of blood vessels during the neural regeneration in more detail. BrdU administration experiments revealed that newly generated NPCs were associated with both newly formed and pre-existing blood vessels in the ischemic striatum, suggesting that the angiogenic environment is not essential for the ...
The zebrafish has become a new model for adult neurogenesis, owing to its abundant neurogenic areas in most brain subdivisions. Radial glia-like cells, actively proliferating cells, and label-retaining progenitors have been described in these areas. In the telencephalon, this complexity is enhanced by an organization of the ventricular zone (VZ) in fast and slow-dividing domains, suggesting the existence of heterogeneous progenitor types. In this work, we studied the expression of various transgenic or immunocytochemical markers for glial cells (gfap:gfp, cyp19a1b:gfp, BLBP, and S100beta), progenitors (nestin:gfp and Sox2), and neuroblasts (PSA-NCAM) in cycling progenitors of the adult zebrafish telencephalon (identified by expression of proliferating cell nuclear antigen (PCNA), MCM5, or bromodeoxyuridine incorporation). We demonstrate the existence of distinct populations of dividing cells at the adult telencephalic VZ. Progenitors of the overall slow-cycling domains express high levels of ...
This family represents the N-terminal region of Pescadillo. Pescadillo protein localises to distinct substructures of the interphase nucleus including nucleoli, the site of ribosome biogenesis. During mitosis pescadillo closely associates with the periphery of metaphase chromosomes and by late anaphase is associated with nucleolus-derived foci and prenucleolar bodies. Blastomeres in mouse embryos lacking pescadillo arrest at morula stages of development, the nucleoli fail to differentiate and accumulation of ribosomes is inhibited. It has been proposed that in mammalian cells pescadillo is essential for ribosome biogenesis and nucleologenesis and that disruption to its function results in cell cycle arrest [1]. This family is often found in conjunction with a PF00533 domain. ...
Stem cells of somatic tissues are hypothesized to protect themselves from mutation and cancer risk through a process of selective segregation of their template DNA strands during asymmetric division. Mouse mammary epithelium contains label-retaining epithelial cells that divide asymmetrically and retain their template DNA. Immunohistochemistry was used in murine mammary glands that had been labeled with [3H]thymidine during allometric growth to investigate the co-expression of DNA label retention and estrogen receptor (ER)-α or progesterone receptor (PR). Using the same methods, we investigated the co-localization of [3H]thymidine and ER-α or PR in mammary tissue from mice that had received treatment with estrogen, progesterone, and prolactin subsequent to a long chase period to identify label-retaining cells. Label-retaining epithelial cells (LRECs) comprised approximately 2.0% of the entire mammary epithelium. ER-α-positive and PR-positive cells represented about 30-40% of the LREC subpopulation.
The p110α and p110β catalytic subunits of phosphoinositide 3-kinase (PI3K) regulate cell cycle events. Marqués et al. found that NIH 3T3 cells overexpressing p110β, but not p110α, divided more rapidly than control cells, whereas cells expressing a catalytically inactive p110β point mutant (Lys805→Arg; K805R) divided more slowly than control cells or cells expressing a catalytically inactive p110α mutant. Specifically, S phase progression was accelerated in cells overexpressing p110β and slowed in cells expressing the K805R mutant, suggesting a role for p110β in DNA elongation. Individual DNA fibers were isolated from synchronously dividing NIH 3T3 cells treated with inhibitors specific for p110α (PIK75) or for p110β (TGX221) and subsequently incubated with bromodeoxyuridine (BrdU). In DNA fibers from TGX221-treated cells, the BrdU-labeled sections were shorter in length and closer together compared with those from PIK75-treated cells, implying that replication fork progression was ...
Bromodeoxyuridine labeling study may be helpful in the diagnosis of meningioma. An elevated bromodeoxyuridine labeling index is suggestive of a rapid growth rate of meningioma and a greater incidence of recurrence following surgical resection. ...
Neurogenesis and apoptosis in the hippocampal dentate gyrus (DG) occur during development and adulthood. However, little is known about how these two processes relate to each other during aging. In this study, we examined apoptosis, proliferation, migration, and survival of newborn cells in the youn …
To determine the effect of protein treatments in cell proliferation, MDA-MB-231 breast cancer cells were first injected into the mammary fat pad of nude mice, and when tumors grew to 10 mm in diameter, mice were treated once only with purified Endo (20 mg/kg), CD (40 mg/kg), or EndoCD (60 mg/kg) proteins plus 500 mg/kg 5-FC, a clinically sufficient dose of 5-FU (15 mg/kg; 1× 5-FU), or 10 times the clinically sufficient dose (150 mg/kg; 10× 5-FU). The choice of 20 mg/kg Endo was based on a previous preclinical study (15) and is also within the dose tested in the phase I clinical trial (ref. 18; 15-600 mg/m2 in human is equivalent to 4.8-194.4 mg/kg in mouse; ref. 30). Tumors were harvested from mice 48 hours after treatment and labeled with bromodeoxyuridine (BrdU) antibody for in vivo BrdU incorporation analysis. The results show that EndoCD/5-FC most significantly reduced cancer cell proliferation (Fig. 2B) compared with all other treatment groups.. The potent inhibitory activity of ...
Figure 3. TAZ protein clusters at the nucleus of proliferating epithelial cells. Conjunctiva epithelial cells were labeled for BrdU (red) and TAZ (green), DAPI was applied as nucleus counter staining dye (blue). White arrows indicate a BrdU positive epithelial cell also stained by high intensity of TAZ protein. Scale bar is 20 µm ...
Immunohistochemical detection of cells labeled with bromodeoxyuridine after neural transplantation.: Pregnant rats were treated with 5-bromo-2-deoxyuridine (Br
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A bromodeoxyuridine analysis". 226: 231-241. Cite journal requires ,journal= (help) Rieger, R; Ladurner, P (2003). "The ...
Induced mutations on the molecular level can be caused by: Chemicals Hydroxylamine Base analogs (e.g., Bromodeoxyuridine (BrdU ...
Kuhn HG, Cooper-Kuhn CM (2007). "Bromodeoxyuridine and the detection of neurogenesis". Curr Pharmaceutical Biotechnol. 8 (3): ...
The effects of bromodeoxyuridine on cell differentiation (conversion of a primitive cell into a more specialized cell) were ... Weintraub, H; Campbell, GL; Holtzer, H (1972). "Identification of a developmental program using bromodeoxyuridine". J Mol Biol ...
During S-phase cells are treated with bromodeoxyuridine (BrdU) which is then incorporated into their nascent DNA, acting as a ... LAMBERT, BO; HANSSON, KERSTIN; LINDSTEN, JAN; STEN, MARGARETA; WERELIUS, BARBRO (12 February 2009). "Bromodeoxyuridine-induced ...
5-Bromouracil Bromodeoxyuridine 5-bromouridine in Linstrom, Peter J.; Mallard, William G. (eds.); NIST Chemistry WebBook, NIST ...
Effect of 5-bromodeoxyuridine on the hemolysin response in rabbits. Proc. Soc. Exp. Biol. Med., 124: 671-75. 1968 With W. H. ...
... based on incorporation of bromodeoxyuridine into single-stranded probes. The method was used to localise satellite repeats very ... Localisation of satellite DNA sequences on human metaphase chromosomes using bromodeoxyuridine-labelled probes. Chromosoma 97: ...
Unlike the commonly used bromodeoxyuridine, EdU detection requires no heat or acid treatment. EdU incorporated into DNA induces ...
High-Frequency Activation in vitro by 5-Iododeoxyuridine and 5-Bromodeoxyuridine". Science. 174 (4005): 155-156. Bibcode: ...
"Identification of active oxalotrophic bacteria by Bromodeoxyuridine DNA labeling in a microcosm soil experiments". FEMS ...
... pulse-labeling chick embryos with bromodeoxyuridine". Journal of Neuroscience. 20 (21): 8021-8030. doi:10.1523/jneurosci.20-21- ...
Moss, Claire; Jackie Hunter, A.; Thorndyke, Michael C. (1998). "Patterns of bromodeoxyuridine incorporation and neuropeptide ...
Neural injections of Bromodeoxyuridine (BrdU) were applied to males of both groups to test for neurogenesis. Analysis showed ...
There it is detected with an ELISA technique: The wells are filled with a solution of a monoclonal antibody to bromo-deoxyuridine ... The DiviTum assay from Biovica International uses another thymidine analogue, bromodeoxyuridine, as substrate to the enzyme. ...
"Induction of endogenous virus and of thymidine kinase by bromodeoxyuridine in cell cultures transformed by Friend virus". ...
This mitotic characteristic is how they are detected by adding Bromodeoxyuridine (BrdU) and staining with anti-BrdU. They have ...
Merrick CJ (December 2015). "Transfection with thymidine kinase permits bromodeoxyuridine labelling of DNA replication in the ...
Base analogs (e.g., Bromodeoxyuridine (BrdU)). *Alkylating agents (e.g., N-ethyl-N-nitrosourea (ENU)). These agents can mutate ...
Bromodeoxyuridine (BrdU) is another thymidine analog that is often used for the detection of proliferating cells in living ...
Hoechst 33342 and 33258 are quenched by bromodeoxyuridine (BrdU), which is commonly used to detect dividing cells. Hoechst ...
"A comparative analysis of intraperitoneal versus intracerebroventricular administration of bromodeoxyuridine for the study of ...
The Giemsa staining was able to stain due to the presence of bromodeoxyuridine analogous base which was introduced to the ...
... bromodeoxyuridine) into the nascent DNA, then fluorescently detecting it. As replication forks spread bidirectionally from ...
... possibly due to inhibition by 5-bromodeoxyuridine and sodium butyrate. Yu, S; et al. (6 May 2017). "The T47D cell line is an ...
Thus, a cell lacking TK is resistant to bromodeoxyuridine (BrdU) and a cell lacking HGPRT is resistant to 6-thioguanine (6-TG) ...
... or parental DNA strands with a DNA label such as tritiated thymidine or bromodeoxyuridine (BrdU). These types of DNA labels ...
... or bromodeoxyuridine-induced carcinogenesis in laboratory stock]. Retrieved 2011-09-08. Kramer, M. J.; Grunberg, E. (1973). " ...
... which are induced by bromodeoxyuridine (BrdU) or distamycin A, an antibiotic that preferentially binds to AT-pairs of DNA. The ...
... of ROSA26 mice beta1-integrin bromodeoxyuridine c-kit (CD117) c-Met C1qR(p) END (CD105) PROM1 (CD133) ALCAM (CD166) ITGB1 (CD29 ...
Bromodeoxyuridine releases gene silencing caused by DNA methylation. BrdU can also be used to identify microorganisms that ... Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU, BUdR, BrdUrd, broxuridine) is a synthetic nucleoside analogue with a chemical ... Dolbeare, F (May 1995). "Bromodeoxyuridine: a diagnostic tool in biology and medicine, Part I: Historical perspectives, ... "Correlation Between Bromodeoxyuridine- Labeling Indices and Patient Prognosis in Cerebral Astrocytic Tumors of Adults". Cancer ...
... bromouracil and bromodeoxyuridine, have been studied. Bromouracil inhibits tentacl ... The effects of bromodeoxyuridine and bromouracil on regeneration inHydra *M. H. Goyns1. & ... Goyns, M.H., Stanisstreet, M. The effects of bromodeoxyuridine and bromouracil on regeneration inHydra . W. Roux Archiv f. ... Lasher, R., Cahn, R. D.: Effects of 5-bromodeoxyuridine on the differentiation of chondrocytes in vitro. Develop. Biol.19, 415- ...
anti-Bromodeoxyuridine/BrdU, Biotin, Clone: SPM537, Novus Biologicals 100 Tests; Biotin Life Sciences:Antibodies:Primary ... Bromodeoxyuridine/BrdU Monoclonal antibody specifically detects Bromodeoxyuridine/BrdU in All Species samples. It is validated ... It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU ...
Sheep Polyclonal Anti-Bromodeoxyuridine/BrdU Antibody Proliferation Marker cited in 15 publications. Validated: ICC/IF, IHC, ... Bromodeoxyuridine/BrdU Antibody Summary. Immunogen. Bromodeoxyuridine (BrdU) coupled to keyhole limpet hemocyanin (KLH). ... BrDU (Bromodeoxyuridine). The thymidine synthetic nucleoside analogue bromodeoxyuridine (BrdU) has a long, colorful history of ... Blogs on Bromodeoxyuridine/BrdU. Check out the latest blog posts on Bromodeoxyuridine/BrdU. ...
Tumor Radiosensitization by Sustained Intratumoral Release of Bromodeoxyuridine. Annie Doiron, Donald T. T. Yapp, Marina ... Tumor Radiosensitization by Sustained Intratumoral Release of Bromodeoxyuridine. Annie Doiron, Donald T. T. Yapp, Marina ... Tumor Radiosensitization by Sustained Intratumoral Release of Bromodeoxyuridine. Annie Doiron, Donald T. T. Yapp, Marina ... Bagshaw M. A., Doggett R. L., Smith K. C., Kaplan H. S., Nelsen T. S. Intra-arterial 5-bromodeoxyuridine and x-ray therapy. Am ...
Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside analogue of thymidine. BrdU is commonly used to ... Retrieved from "https://openwetware.org/mediawiki/index.php?title=Bromodeoxyuridine_(BrdU)&oldid=379477" ...
Mouse Monoclonal Anti-Bromodeoxyuridine/BrdU Antibody (BRD494) [Biotin]. Proliferation Marker. Validated: ICC/IF, IHC-Fr, IHC-P ... Check out the latest blog posts on Bromodeoxyuridine/BrdU.. BrDU (Bromodeoxyuridine). The thymidine synthetic nucleoside ... Bromodeoxyuridine/BrdU Antibody (BRD494) [Biotin] Summary. Immunogen. Bromodeoxyuridine (BrdU) conjugated to KLH ... Reviews for Bromodeoxyuridine/BrdU Antibody (NBP2-34566B) (0) There are no reviews for Bromodeoxyuridine/BrdU Antibody (NBP2- ...
High-speed photodamage cell selection using bromodeoxyuridine/Hoechst 33342 photosensitized cell killing†‡. Authors. *. Hans ...
HPLC/UV or HPLC/32P-postlabeling methods for quantification of bromodeoxyuridine in DNA. M. Vijayaraj Reddy, John E. Barnum, ... The extent of incorporation of exogenously administered bromodeoxyuridine (BrdU) into DNA is an indicator of the rate of DNA ... HPLC/UV or HPLC/32P-postlabeling methods for quantification of bromodeoxyuridine in DNA ... HPLC/UV or HPLC/32P-postlabeling methods for quantification of bromodeoxyuridine in DNA ...
Use of Bromodeoxyuridine to Study White Blood Cell Replication and Survival in HIV-Infected Patients. The safety and scientific ...
Mouse Monoclonal anti-BrdU (Bromodeoxyuridine) (BRD469) This antibody reacts with Bromodeoxyuridine (BrdU) in single stranded ... This antibody reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded ...
Monoclonal Anti-Bromodeoxyuridine (Clone BU6-4). Monoclonal Anti-Bromodeoxyuridine (Clone BU6-4) is often used in cell cycle ... The monoclonal anti-bromodeoxyuridine antibody can be used in Western blot analysis under non-reducing and non-heating ... Immunogen: Bromodeoxyuridine-BSA conjugate *Immunologic host: BALB/c mouse *antibody subclass: IgG1 *Antibody titer: 1:2,000 ... analysis studies; the level of bromodeoxyuridine (BrdU) incorporation into DNA is a direct parameter of cell number and growth ...
Effects of bromodeoxyuridine, cytosine arabinoside and Colcemid upon in vitro development of mouse blastocysts ... Effects of bromodeoxyuridine, cytosine arabinoside and Colcemid upon in vitro development of mouse blastocysts ... Effects of bromodeoxyuridine, cytosine arabinoside and Colcemid upon in vitro development of mouse blastocysts ... Effects of bromodeoxyuridine, cytosine arabinoside and Colcemid upon in vitro development of mouse blastocysts ...
Abstract The interaction of a nucleoside analogue bromodeoxyuridine (BrdU) with human serum albumin (HSA) was studied to ... Bromodeoxyuridine Human serum albumin Drug-protein interaction Fluorescence quenching Molecular modelling Electronic ... The interaction of a nucleoside analogue bromodeoxyuridine (BrdU) with human serum albumin (HSA) was studied to investigate the ... Biophysical and computational approaches to unravel the molecular interaction mechanism of bromodeoxyuridine, a proliferative ...
Effect of 5-bromodeoxyuridine on the appearance of the liver isoenzyme of pyruvate kinase Biochem J (June, 1984) ... Control and bromodeoxyuridine-containing rat-embryo-cell DNA were digested by the restriction endonucleases Hpa II and Msp I ... base-specific affinity chromatography of rat-embryo DNA sequences disproportionately enriched in virogenic bromodeoxyuridine ... base-specific affinity chromatography of rat-embryo DNA sequences disproportionately enriched in virogenic bromodeoxyuridine. ...
Cutress, R.I., Mullee, M.A. and Rew, D.A. (2002) Clinical outcome and bromodeoxyuridine derived proliferation indices in 100 ... Clinical outcome and bromodeoxyuridine derived proliferation indices in 100 colonic and rectal carcinomas ... Aim: In vivo labelling of human colonic and rectal tumours with bromodeoxyuridine (BrdUrd) and analysis by flow cytometry (FCM ... Aim: In vivo labelling of human colonic and rectal tumours with bromodeoxyuridine (BrdUrd) and analysis by flow cytometry (FCM ...
The development of a monoclonal antibody against 5-Bromodeoxyuridine and its applications in research microfilm. Welcome to the ... The development of a monoclonal antibody against 5-Bromodeoxyuridine and its applications in research microfilm. Schmeda, Peter ... The development of a monoclonal antibody against 5-Bromodeoxyuridine and its applications in research microfilm. ...
Bioavailability of bromodeoxyuridine in dogs and toxicity in rats. by Surasak Phuphanich et al. ... A New Method for In Vitro Detection of Bromodeoxyuridine in Serum: A Proof of Concept in a Songbird Species, the Canary. * ... Bioavailability of bromodeoxyuridine in dogs and toxicity in rats.. @article{Phuphanich1985BioavailabilityOB, title={ ... Bioavailability of bromodeoxyuridine in dogs and toxicity in rats.}, author={Surasak Phuphanich and V. A. Levin}, journal={ ...
Bromodeoxyuridine (BrdU) (Proliferation Marker) Antibody - With BSA and Azide, Mouse Monoclonal Antibody [Clone BRD469 + BRD494 ... Bromodeoxyuridine (BrdU) (Proliferation Marker) Antibody - With BSA and Azide is for research use only and not for use in ... It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU ... Bromodeoxyuridine (BrdU) (Proliferation Marker) Antibody - With BSA and Azide. Mouse Monoclonal Antibody [Clone BRD469 + BRD494 ...
Possible utilization of bromodeoxyuridine triphosphate for site-directed mutagenesis. M Muller, J Martial, W G Verly ... Possible utilization of bromodeoxyuridine triphosphate for site-directed mutagenesis Message Subject (Your Name) has forwarded ...
f Inhibition of Sporulation in Bacillus subtilis by Bromodeoxyuridine and the Effect on DNA Replication ... Inhibition of Sporulation in Bacillus subtilis by Bromodeoxyuridine and the Effect on DNA Replication, Page 1 of 1 ...
... describes an improved immunochemical procedure for staining cells in suspension for amount of incorporated bromodeoxyuridine ( ... Improved bromodeoxyuridine/DNA analysis by anti-BudR monoclonal antibody versus right angle light scatter. Marco Vitale, Luca M ... Cytochemistry for bromodeoxyuridine/DNA analysis: stoichiometry and sensitivity.. @article{Dolbeare1985CytochemistryFB, title={ ... Cytochemistry for bromodeoxyuridine/DNA analysis: stoichiometry and sensitivity.}, author={Frank Dolbeare and Wolfgang Beisker ...
Mouse anti BrdU (bromodeoxyuridine). Catalogue number: X1028. Synonyms. Anti-Bromodeoxyuridine Mouse MoBU Monoclonal Antibody ... Bromodeoxyuridine conjugated to Helix Pomatia Haemocyanin used to immunize BALB/c mice Purification Method. Protein A/G ... Application of bromodeoxyuridine (BrdU) and anti-BrdU monoclonal antibody for the in vivo analysis of proliferative ... Oncology 1991, 48, 285-289 2. Wilson, G., Cell kinetic studies using a monoclonal antibody to bromodeoxyuridine. Methods Mol. ...
Abnormal DNA synthesis in polyamine deficient cells revealed by bromodeoxyuridine-flow cytometry technique. Research output: ... the cells were labelled for 15-120 min with the thymidine analogue bromodeoxyuridine (BrdUrd) and they were then fixed directly ...
Mixed lymphocyte reactions evaluated by means of bromodeoxyuridine incorporation. A. Bontadini, R. Conte, A. Dinota, D. ... Mixed lymphocyte reactions evaluated by means of bromodeoxyuridine incorporation. / Bontadini, A.; Conte, R.; Dinota, A.; ... title = "Mixed lymphocyte reactions evaluated by means of bromodeoxyuridine incorporation",. author = "A. Bontadini and R. ... Mixed lymphocyte reactions evaluated by means of bromodeoxyuridine incorporation. Haematologica. 1990;75(1):7-11. ...
5-bromodeoxyuridine;. En,. embryonic day n;. FITC,. fluorescein isothiocyanate;. Pn,. postnatal day n;. MAP2,. microtubule- ... 5-Bromodeoxyuridine (BrdUrd) Labeling.. Pregnant mice were injected intraperitoneally with BrdUrd (Sigma; 10 mg/ml in PBS) at ... Labeling experiments with 5-bromodeoxyuridine demonstrated that the labeled cells in the stratum pyramidale of the CR-50- ...
Bromodeoxyuridine incorporation. CHB50 cells were cultured on 96-well microtiter plates and differentiated for 5 consecutive ...
Bromodeoxyuridine-5 (BrdU) Sheep Polyclonal Antibody This product is available for purchase offline. Please contact us to ...
MBLのおすすめAnti-Bromodeoxyuridine mAb(Monoclonal, 2B1)。 使用法:Immunoprecipitation, Flow Cytometry, Immunocytochemistry, ... MI-11-5 Anti-Bromodeoxyuridine mAb-PE. M075-3 Mouse IgG1 (isotype control). RN1011 RiboTrap Kit. RN1008 BRIC Kit. PM077 Anti-5- ...
Bromo-deoxyuridine labeling.. After partial pancreatectomy, some animals were injected intraperitoneally with bromo- ...
  • This study includes 182 patients with intracranial gliomas who received bromodeoxyuridine (BUdR), 200 mg/sq m intravenously, at the time of craniotomy but before tumor biopsy. (thejns.org)
  • One hundred fifty-two intracranial gliomas of various types were reviewed in order to correlate the histopathological features with the proliferative potential of each tumor as reflected by the bromodeoxyuridine (BUdR) labeling index (LI). (thejns.org)
  • Thymidine (Tdr) and its analogues 5-fluorodeoxyuridine (FUdr) and 5-bromodeoxyuridine (BUdr) inhibited the development of antibody-producing cells (PFC) in cultures of explants of rabbit spleen stimulated with sheep red cells in vitro. (biomedsearch.com)
  • Bromodeoxyuridine/BrdU Monoclonal antibody specifically detects Bromodeoxyuridine/BrdU in All Species samples. (fishersci.com)
  • Immunocytochemistry/ Immunofluorescence: Bromodeoxyuridine/BrdU Antibody [NB500-235] - IF analysis of BrdU in Proteinase K pretreated mouse lacrimal gland. (novusbio.com)
  • There are currently no images for Bromodeoxyuridine/BrdU Antibody (NBP2-34566B). (novusbio.com)
  • The monoclonal anti-bromodeoxyuridine antibody can be used in Western blot analysis under non-reducing and non-heating conditions and in flow cytometry applications. (clontech.com)
  • Application of bromodeoxyuridine (BrdU) and anti-BrdU monoclonal antibody for the in vivo analysis of proliferative characteristics of human leukemia cells in bone marrows. (exalpha.com)
  • Wilson, G., Cell kinetic studies using a monoclonal antibody to bromodeoxyuridine. (exalpha.com)
  • A monoclonal antibody to bromodeoxyuridine (BrdUrd) incorporated into DNA allowed visualization of sister chromatid exchanges (SCE) when as little as 0.6% of the thymine in a single DNA strand has been substituted. (elsevier.com)
  • First, the use of 3 H-thymidine, a radioactive nucleotide used to study proliferation when incorporated into the cells during the S phase of the cell cycle, was replaced by its analog, bromodeoxyuridine (BrdU), which could be detected by a specific antibody. (pubmedcentralcanada.ca)
  • The extent of incorporation of exogenously administered bromodeoxyuridine (BrdU) into DNA is an indicator of the rate of DNA synthesis and cell proliferation. (aacrjournals.org)
  • the level of bromodeoxyuridine (BrdU) incorporation into DNA is a direct parameter of cell number and growth. (clontech.com)
  • Gobbi, M. / Mixed lymphocyte reactions evaluated by means of bromodeoxyuridine incorporation . (elsevier.com)
  • Hill, BT , Augenlicht, LH & Baserga, R 1973, ' Increased incorporation of 5-bromodeoxyuridine into the DNA of proliferating tissues in partially hepatectomised mice ', FEBS Letters , vol. 37, no. 2, pp. 298-302. (elsevier.com)
  • Cell proliferation was assayed by cell counts and bromodeoxyuridine (BrdU) Incorporation. (molvis.org)
  • In vivo treatment with PTN (20 ng/g) increased bromodeoxyuridine incorporation (by 2.24-fold) and PCNA expression (by 1.71-fold) during day 7 to day 14, indicating that PTN induces cell proliferation in mouse heart. (pubmedcentralcanada.ca)
  • Cell proliferation and viability were investigated using cell counts, 5′-bromodeoxyuridine incorporation (BrdU assay) and tetrazolium reduction (MTT assay). (bmj.com)
  • We have now investigated, in the same tumor model, radiosensitization by the thymidine analogue bromodeoxyuridine (BrdUrd). (aacrjournals.org)
  • Aim: In vivo labelling of human colonic and rectal tumours with bromodeoxyuridine (BrdUrd) and analysis by flow cytometry (FCM) allows the labelling index (LI), S phase duration (Ts) and the potential doubling time (Tpot) of the tumour to be estimated in vivo. (soton.ac.uk)
  • This report describes an improved immunochemical procedure for staining cells in suspension for amount of incorporated bromodeoxyuridine (BrdUrd) and total DNA. (semanticscholar.org)
  • At 1-4 days after seeding, the cells were labelled for 15-120 min with the thymidine analogue bromodeoxyuridine (BrdUrd) and they were then fixed directly after the labelling period. (lu.se)
  • Bromodeoxyuridine (BrdU) coupled to keyhole limpet hemocyanin (KLH). (novusbio.com)
  • Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU ) is a synthetic nucleoside analogue of thymidine. (openwetware.org)
  • The thymidine synthetic nucleoside analogue bromodeoxyuridine (BrdU) has a long, colorful history of repeated use in molecular and cytokinetic studies, as detailed in reviews by Vanderlaan and Dolbeare (1,2). (novusbio.com)
  • BrdU (Bromodeoxyuridine) is a synthetic nucleoside that is incorporated into actively replicating cells' DNA. (novusbio.com)
  • The interaction of a nucleoside analogue bromodeoxyuridine (BrdU) with human serum albumin (HSA) was studied to investigate the binding phenomenon and analyse the protein conformation upon BrdU binding. (springer.com)
  • To elucidate the mechanism of bromodeoxyuridine (BrdU) induced cellular senescence, we treated HeLa cells with D4476, a potent and specific inhibitor of casein kinase 1(CK1). (bio-journal.com)
  • Rats received repeated intraperitoneal injections of the cell proliferation-specific marker 5-bromodeoxyuridine (BrdU) after stroke induction. (ahajournals.org)
  • The distribution of DNA synthesising cells in the crypts of the epithelium in human small and large bowel after injection of bromodeoxyuridine into patients has been studied in relation to the position of the cells in the crypt using immunohistochemistry. (openrepository.com)
  • It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. (fishersci.com)
  • This product reacts specifically with bromodeoxyuridine. (clontech.com)
  • Cutress, R.I. , Mullee, M.A. and Rew, D.A. (2002) Clinical outcome and bromodeoxyuridine derived proliferation indices in 100 colonic and rectal carcinomas. (soton.ac.uk)
  • Morphometric measurements and measurements of cell proliferation by bromodeoxyuridine index analysis were performed at both arterial and venous anastomoses. (ahajournals.org)
  • Proliferation in human gastrointestinal epithelium using bromodeoxyuridine in vivo: data for different sites, proximity to a tumour, and polyposis coli. (openrepository.com)
  • Measurement of in vivo proliferation in human colorectal mucosa using bromodeoxyuridine. (openrepository.com)
  • The effects on tentacle regeneration in Hydra of two DNA-antimetabolites, bromouracil and bromodeoxyuridine, have been studied. (springer.com)
  • The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. (abcam.com)
  • Replication not inhibited by 5-bromodeoxyuridine. (cdc.gov)
  • Labeling experiments with 5-bromodeoxyuridine demonstrated that the labeled cells in the stratum pyramidale of the CR-50-treated mice were distributed in a pattern similar to that of reeler . (pnas.org)
  • Bromodeoxyuridine (BrdU) is a thymidine analog and it incorporates into the DNA only if the cells are at S-phase. (researchsquare.com)
  • During S-phase cells are treated with bromodeoxyuridine (BrdU) which is then incorporated into their nascent DNA, acting as a substitute for thymidine. (wikipedia.org)
  • Cytochemistry for bromodeoxyuridine/DNA analysis: stoichiometry and sensitivity. (semanticscholar.org)
  • Lasher, R., Cahn, R. D.: Effects of 5-bromodeoxyuridine on the differentiation of chondrocytes in vitro. (springer.com)
  • Coleman, J. R., Coleman, W. A., Hartline, E. J. H.: A clonal study of the reversible inhibition of muscle differentiation by the halogenated thymidine analogue 5-bromodeoxyuridine. (springer.com)
  • Holtzer, H.: Cell differentiation in the presence of bromodeoxyuridine. (springer.com)
  • The effects of bromodeoxyuridine on cell differentiation (conversion of a primitive cell into a more specialized cell) were also analyzed. (wikipedia.org)
  • An elevated bromodeoxyuridine labeling index is suggestive of a rapid growth rate of meningioma and a greater incidence of recurrence following surgical resection. (wikidoc.org)
  • There are currently no images for Bromodeoxyuridine/BrdU Antibody (NBP2-68041C). (novusbio.com)
  • FAQs for Bromodeoxyuridine/BrdU Antibody (NBP2-68041C). (novusbio.com)
  • A number of DLX-2-expressing cells, but not all, can be labeled with bromodeoxyuridine (BrdU). (jneurosci.org)
  • 2013), and bromodeoxyuridine (BrdU) (Long et?al. (aqualogy.org)
  • The effects of perforant path stimulation on GC neurogenesis is assessed by administering bromodeoxyuridine (BrdU), a thymidine analog incorporated only in dividing cells, in conjunction with unbiased stereology and fluorescent immuno-cytochemistry to quantify BrdU-positive cells, IEG protein expression and markers of GC development (progenitor, immature and mature). (grantome.com)
  • Therefore, solutions to assay DNA replication, including quantitative PCR (qPCR) or immunofluorescence from the nucleotide analog bromodeoxyuridine (BrdU) to imagine recently replicated DNA, may be used to assess the specific timing of replication occasions and to split initiation and elongation stages at specific amplicons. (ac-devd-cho.com)
  • In the present study, the anticancer effects of TP4O against the colorectal cancer (CRC) cell lines HCT116 and RKO were evaluated using WST\u20118 and bromodeoxyuridine assays. (nii.ac.jp)