Bromodeoxyuridine: A nucleoside that substitutes for thymidine in DNA and thus acts as an antimetabolite. It causes breaks in chromosomes and has been proposed as an antiviral and antineoplastic agent. It has been given orphan drug status for use in the treatment of primary brain tumors.Cell Division: The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.Antimetabolites: Drugs that are chemically similar to naturally occurring metabolites, but differ enough to interfere with normal metabolic pathways. (From AMA Drug Evaluations Annual, 1994, p2033)Bromouracil: 5-Bromo-2,4(1H,3H)-pyrimidinedione. Brominated derivative of uracil that acts as an antimetabolite, substituting for thymine in DNA. It is used mainly as an experimental mutagen, but its deoxyriboside (BROMODEOXYURIDINE) is used to treat neoplasms.Cell Proliferation: All of the processes involved in increasing CELL NUMBER including CELL DIVISION.ThymidineCell Count: The number of CELLS of a specific kind, usually measured per unit volume or area of sample.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).DNA Replication: The process by which a DNA molecule is duplicated.Cell Cycle: The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.Neurogenesis: Formation of NEURONS which involves the differentiation and division of STEM CELLS in which one or both of the daughter cells become neurons.Immunohistochemistry: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.S Phase: Phase of the CELL CYCLE following G1 and preceding G2 when the entire DNA content of the nucleus is replicated. It is achieved by bidirectional replication at multiple sites along each chromosome.Proliferating Cell Nuclear Antigen: Nuclear antigen with a role in DNA synthesis, DNA repair, and cell cycle progression. PCNA is required for the coordinated synthesis of both leading and lagging strands at the replication fork during DNA replication. PCNA expression correlates with the proliferation activity of several malignant and non-malignant cell types.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Idoxuridine: An analog of DEOXYURIDINE that inhibits viral DNA synthesis. The drug is used as an antiviral agent.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Mitotic Index: An expression of the number of mitoses found in a stated number of cells.Stem Cells: Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.Dentate Gyrus: GRAY MATTER situated above the GYRUS HIPPOCAMPI. It is composed of three layers. The molecular layer is continuous with the HIPPOCAMPUS in the hippocampal fissure. The granular layer consists of closely arranged spherical or oval neurons, called GRANULE CELLS, whose AXONS pass through the polymorphic layer ending on the DENDRITES of PYRAMIDAL CELLS in the hippocampus.Time Factors: Elements of limited time intervals, contributing to particular results or situations.TritiumCell Line: Established cell cultures that have the potential to propagate indefinitely.Sister Chromatid Exchange: An exchange of segments between the sister chromatids of a chromosome, either between the sister chromatids of a meiotic tetrad or between the sister chromatids of a duplicated somatic chromosome. Its frequency is increased by ultraviolet and ionizing radiation and other mutagenic agents and is particularly high in BLOOM SYNDROME.Rats, Sprague-Dawley: A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.Mitosis: A type of CELL NUCLEUS division by means of which the two daughter nuclei normally receive identical complements of the number of CHROMOSOMES of the somatic cells of the species.Neurons: The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.Mice, Inbred C57BLCell Survival: The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.Staining and Labeling: The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.Flow Cytometry: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.Rats, Inbred F344Nestin: A type VI intermediate filament protein expressed mostly in nerve cells where it is associated with the survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.Regeneration: The physiological renewal, repair, or replacement of tissue.Ultraviolet Rays: That portion of the electromagnetic spectrum immediately below the visible range and extending into the x-ray frequencies. The longer wavelengths (near-UV or biotic or vital rays) are necessary for the endogenous synthesis of vitamin D and are also called antirachitic rays; the shorter, ionizing wavelengths (far-UV or abiotic or extravital rays) are viricidal, bactericidal, mutagenic, and carcinogenic and are used as disinfectants.Deoxyuridine: 2'-Deoxyuridine. An antimetabolite that is converted to deoxyuridine triphosphate during DNA synthesis. Laboratory suppression of deoxyuridine is used to diagnose megaloblastic anemias due to vitamin B12 and folate deficiencies.Animals, Newborn: Refers to animals in the period of time just after birth.Epithelial Cells: Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.Nucleic Acid Renaturation: The reformation of all, or part of, the native conformation of a nucleic acid molecule after the molecule has undergone denaturation.Azaguanine: One of the early purine analogs showing antineoplastic activity. It functions as an antimetabolite and is easily incorporated into ribonucleic acids.Thymidine Kinase: An enzyme that catalyzes the conversion of ATP and thymidine to ADP and thymidine 5'-phosphate. Deoxyuridine can also act as an acceptor and dGTP as a donor. (From Enzyme Nomenclature, 1992) EC 2.7.1.21.Aminopterin: A folic acid derivative used as a rodenticide that has been shown to be teratogenic.Thymine Nucleotides: Phosphate esters of THYMIDINE in N-glycosidic linkage with ribose or deoxyribose, as occurs in nucleic acids. (From Dorland, 28th ed, p1154)Glial Fibrillary Acidic Protein: An intermediate filament protein found only in glial cells or cells of glial origin. MW 51,000.Radiation Effects: The effects of ionizing and nonionizing radiation upon living organisms, organs and tissues, and their constituents, and upon physiologic processes. It includes the effect of irradiation on food, drugs, and chemicals.Ki-67 Antigen: A CELL CYCLE and tumor growth marker which can be readily detected using IMMUNOCYTOCHEMISTRY methods. Ki-67 is a nuclear antigen present only in the nuclei of cycling cells.Floxuridine: An antineoplastic antimetabolite that is metabolized to fluorouracil when administered by rapid injection; when administered by slow, continuous, intra-arterial infusion, it is converted to floxuridine monophosphate. It has been used to treat hepatic metastases of gastrointestinal adenocarcinomas and for palliation in malignant neoplasms of the liver and gastrointestinal tract.Cell Movement: The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.Hippocampus: A curved elevation of GRAY MATTER extending the entire length of the floor of the TEMPORAL HORN of the LATERAL VENTRICLE (see also TEMPORAL LOBE). The hippocampus proper, subiculum, and DENTATE GYRUS constitute the hippocampal formation. Sometimes authors include the ENTORHINAL CORTEX in the hippocampal formation.Cell Nucleus: Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Carcinogens: Substances that increase the risk of NEOPLASMS in humans or animals. Both genotoxic chemicals, which affect DNA directly, and nongenotoxic chemicals, which induce neoplasms by other mechanism, are included.DNA, Neoplasm: DNA present in neoplastic tissue.Disease Models, Animal: Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.Fluorescent Antibody Technique: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.Deoxyuracil Nucleotides: Uracil nucleotides which contain deoxyribose as the sugar moiety.Autoradiography: The making of a radiograph of an object or tissue by recording on a photographic plate the radiation emitted by radioactive material within the object. (Dorland, 27th ed)Hyperplasia: An increase in the number of cells in a tissue or organ without tumor formation. It differs from HYPERTROPHY, which is an increase in bulk without an increase in the number of cells.In Situ Nick-End Labeling: An in situ method for detecting areas of DNA which are nicked during APOPTOSIS. Terminal deoxynucleotidyl transferase is used to add labeled dUTP, in a template-independent manner, to the 3 prime OH ends of either single- or double-stranded DNA. The terminal deoxynucleotidyl transferase nick end labeling, or TUNEL, assay labels apoptosis on a single-cell level, making it more sensitive than agarose gel electrophoresis for analysis of DNA FRAGMENTATION.Liver Regeneration: Repair or renewal of hepatic tissue.Centrifugation, Density Gradient: Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Rats, Wistar: A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.Culture Techniques: Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.Intermediate Filament Proteins: Filaments 7-11 nm in diameter found in the cytoplasm of all cells. Many specific proteins belong to this group, e.g., desmin, vimentin, prekeratin, decamin, skeletin, neurofilin, neurofilament protein, and glial fibrillary acid protein.Fibroblasts: Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.Intestinal Mucosa: Lining of the INTESTINES, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. In the SMALL INTESTINE, the mucosa is characterized by a series of folds and abundance of absorptive cells (ENTEROCYTES) with MICROVILLI.Papilloma: A circumscribed benign epithelial tumor projecting from the surrounding surface; more precisely, a benign epithelial neoplasm consisting of villous or arborescent outgrowths of fibrovascular stroma covered by neoplastic cells. (Stedman, 25th ed)Dose-Response Relationship, Drug: The relationship between the dose of an administered drug and the response of the organism to the drug.Neuroglia: The non-neuronal cells of the nervous system. They not only provide physical support, but also respond to injury, regulate the ionic and chemical composition of the extracellular milieu, participate in the BLOOD-BRAIN BARRIER and BLOOD-RETINAL BARRIER, form the myelin insulation of nervous pathways, guide neuronal migration during development, and exchange metabolites with neurons. Neuroglia have high-affinity transmitter uptake systems, voltage-dependent and transmitter-gated ion channels, and can release transmitters, but their role in signaling (as in many other functions) is unclear.Neural Stem Cells: Self-renewing cells that generate the main phenotypes of the nervous system in both the embryo and adult. Neural stem cells are precursors to both NEURONS and NEUROGLIA.Interphase: The interval between two successive CELL DIVISIONS during which the CHROMOSOMES are not individually distinguishable. It is composed of the G phases (G1 PHASE; G0 PHASE; G2 PHASE) and S PHASE (when DNA replication occurs).RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.4-Nitroquinoline-1-oxide: A potent mutagen and carcinogen. This compound and its metabolite 4-HYDROXYAMINOQUINOLINE-1-OXIDE bind to nucleic acids. It inactivates bacteria but not bacteriophage.Cricetinae: A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.G1 Phase: The period of the CELL CYCLE preceding DNA REPLICATION in S PHASE. Subphases of G1 include "competence" (to respond to growth factors), G1a (entry into G1), G1b (progression), and G1c (assembly). Progression through the G1 subphases is effected by limiting growth factors, nutrients, or inhibitors.Neoplasms, Experimental: Experimentally induced new abnormal growth of TISSUES in animals to provide models for studying human neoplasms.Deoxyribonucleotides: A purine or pyrimidine base bonded to a DEOXYRIBOSE containing a bond to a phosphate group.Kinetics: The rate dynamics in chemical or physical systems.Cell Transformation, Neoplastic: Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.Nucleic Acid Denaturation: Disruption of the secondary structure of nucleic acids by heat, extreme pH or chemical treatment. Double strand DNA is "melted" by dissociation of the non-covalent hydrogen bonds and hydrophobic interactions. Denatured DNA appears to be a single-stranded flexible structure. The effects of denaturation on RNA are similar though less pronounced and largely reversible.Tyrosine Transaminase: An enzyme that catalyzes the conversion of L-TYROSINE and 2-oxoglutarate to 4-hydroxyphenylpyruvate and L-GLUTAMATE. It is a pyridoxal-phosphate protein. L-PHENYLALANINE is hydroxylated to L-tyrosine. The mitochondrial enzyme may be identical with ASPARTATE AMINOTRANSFERASES (EC 2.6.1.1.). Deficiency of this enzyme may cause type II Tyrosinemia (see TYROSINEMIAS). EC 2.6.1.5.Chick Embryo: The developmental entity of a fertilized chicken egg (ZYGOTE). The developmental process begins about 24 h before the egg is laid at the BLASTODISC, a small whitish spot on the surface of the EGG YOLK. After 21 days of incubation, the embryo is fully developed before hatching.Mice, Transgenic: Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.Mice, Knockout: Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.Phenotype: The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.Vimentin: An intermediate filament protein found in most differentiating cells, in cells grown in tissue culture, and in certain fully differentiated cells. Its insolubility suggests that it serves a structural function in the cytoplasm. MW 52,000.Fibroblast Growth Factor 2: A single-chain polypeptide growth factor that plays a significant role in the process of WOUND HEALING and is a potent inducer of PHYSIOLOGIC ANGIOGENESIS. Several different forms of the human protein exist ranging from 18-24 kDa in size due to the use of alternative start sites within the fgf-2 gene. It has a 55 percent amino acid residue identity to FIBROBLAST GROWTH FACTOR 1 and has potent heparin-binding activity. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor (FIBROBLAST GROWTH FACTOR 1).Phosphopyruvate Hydratase: A hydro-lyase that catalyzes the dehydration of 2-phosphoglycerate to form PHOSPHOENOLPYRUVATE. Several different isoforms of this enzyme exist, each with its own tissue specificity.Lateral Ventricles: Cavity in each of the CEREBRAL HEMISPHERES derived from the cavity of the embryonic NEURAL TUBE. They are separated from each other by the SEPTUM PELLUCIDUM, and each communicates with the THIRD VENTRICLE by the foramen of Monro, through which also the choroid plexuses (CHOROID PLEXUS) of the lateral ventricles become continuous with that of the third ventricle.Ependyma: A thin membrane that lines the CEREBRAL VENTRICLES and the central canal of the SPINAL CORD.UridineOrgan Size: The measurement of an organ in volume, mass, or heaviness.Colon: The segment of LARGE INTESTINE between the CECUM and the RECTUM. It includes the ASCENDING COLON; the TRANSVERSE COLON; the DESCENDING COLON; and the SIGMOID COLON.Clone Cells: A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)Liver Neoplasms, Experimental: Experimentally induced tumors of the LIVER.Tumor Cells, Cultured: Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.Cytarabine: A pyrimidine nucleoside analog that is used mainly in the treatment of leukemia, especially acute non-lymphoblastic leukemia. Cytarabine is an antimetabolite antineoplastic agent that inhibits the synthesis of DNA. Its actions are specific for the S phase of the cell cycle. It also has antiviral and immunosuppressant properties. (From Martindale, The Extra Pharmacopoeia, 30th ed, p472)DNA, Viral: Deoxyribonucleic acid that makes up the genetic material of viruses.Mice, Inbred Strains: Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.

The incorporation of 5-iodo-2'-deoxyuridine into the DNA of HeLa cells and the induction of alkaline phosphatase activity. (1/3497)

Inhibition of DNA synthesis during the period of exposure of HeLa cells to 5-iodo-2'-deoxyuridine (IUdR) inhibited the induction of alkaline phosphatase activity. This finding, taken together with previous findings that IUdR did not induce alkaline phosphatase activity in the presence of 2-fold molar excess thymidinemonstrated that IUdR incorporation into DNA is correlated with the increase in alkaline phosphatase activity. With the exception of an interim period described in the text, induction of alkaline phosphatase activity was linearly related to medium concentrations of IUdR of up to at least 3 muM. However, the extent of IUdR substitution in DNA did not appear to be related to the degree of enzyme induction. Alkaline phosphatase activity continued to increase at medium concentrations of IUdR from 1 to 3 muM, while little further substitution of DNA occurred.  (+info)

High-throughput screening of small molecules in miniaturized mammalian cell-based assays involving post-translational modifications. (2/3497)

BACKGROUND: Fully adapting a forward genetic approach to mammalian systems requires efficient methods to alter systematically gene products without prior knowledge of gene sequences, while allowing for the subsequent characterization of these alterations. Ideally, these methods would also allow function to be altered in a temporally controlled manner. RESULTS: We report the development of a miniaturized cell-based assay format that enables a genetic-like approach to understanding cellular pathways in mammalian systems using small molecules, rather than mutations, as the source of gene-product alterations. This whole-cell immunodetection assay can sensitively detect changes in specific cellular macromolecules in high-density arrays of mammalian cells. Furthermore, it is compatible with screening large numbers of small molecules in nanoliter to microliter culture volumes. We refer to this assay format as a 'cytoblot', and demonstrate the use of cytoblotting to monitor biosynthetic processes such as DNA synthesis, and post-translational processes such as acetylation and phosphorylation. Finally, we demonstrate the applicability of these assays to natural-product screening through the identification of marine sponge extracts exhibiting genotype-specific inhibition of 5-bromodeoxyuridine incorporation and suppression of the anti-proliferative effect of rapamycin. CONCLUSIONS: We show that cytoblots can be used for high-throughput screening of small molecules in cell-based assays. Together with small-molecule libraries, the cytoblot assay can be used to perform chemical genetic screens analogous to those used in classical genetics and thus should be applicable to understanding a wide variety of cellular processes, especially those involving post-transitional modifications.  (+info)

Herpetic keratitis. Proctor Lecture. (3/3497)

Although much needs to be learned about the serious clinical problem of herpes infection of the cornea, we have come a long way. We now have effective topical antiviral drugs. We have animal models which, with a high degree of reliability, clearly predict the effect to be expected clinically in man, as well as the toxicity. We have systemically active drugs and the potential of getting highly active, potent, completely selective drugs, with the possibility that perhaps the source of viral reinfection can be eradicated. The biology of recurrent herpes and stromal disease is gradually being understood, and this understanding may result in new and better therapy of this devastating clinical disease.  (+info)

Immunochemical detection and isolation of DNA from metabolically active bacteria. (4/3497)

Most techniques used to assay the growth of microbes in natural communities provide no information on the relationship between microbial productivity and community structure. To identify actively growing bacteria, we adapted a technique from immunocytochemistry to detect and selectively isolate DNA from bacteria incorporating bromodeoxyuridine (BrdU), a thymidine analog. In addition, we developed an immunocytochemical protocol to visualize BrdU-labeled microbial cells. Cultured bacteria and natural populations of aquatic bacterioplankton were pulse-labeled with exogenously supplied BrdU. Incorporation of BrdU into microbial DNA was demonstrated in DNA dot blots probed with anti-BrdU monoclonal antibodies and either peroxidase- or Texas red-conjugated secondary antibodies. BrdU-containing DNA was physically separated from unlabeled DNA by using antibody-coated paramagnetic beads, and the identities of bacteria contributing to both purified, BrdU-containing fractions and unfractionated, starting-material DNAs were determined by length heterogeneity PCR (LH-PCR) analysis. BrdU-containing DNA purified from a mixture of DNAs from labeled and unlabeled cultures showed >90-fold enrichment for the labeled bacterial taxon. The LH-PCR profile for BrdU-containing DNA from a labeled, natural microbial community differed from the profile for the community as a whole, demonstrating that BrdU was incorporated by a taxonomic subset of the community. Immunocytochemical detection of cells with BrdU-labeled DNA was accomplished by in situ probing with anti-BrdU monoclonal antibodies and Texas red-labeled secondary antibodies. Using this suite of techniques, microbial cells incorporating BrdU into their newly synthesized DNA can be quantified and the identities of these actively growing cells can be compared to the composition of the microbial community as a whole. Since not all strains tested could incorporate BrdU, these methods may be most useful when used to gain an understanding of the activities of specific species in the context of their microbial community.  (+info)

Proteolipid protein gene product can be secreted and exhibit biological activity during early development. (5/3497)

A gene encoding myelin proteolipid protein (PLP) and its smaller isoform DM20 is expressed at least 1 week before myelination. Mutations within the gene cause abnormalities in the development of premyelinating oligodendrocytes, resulting in hypomyelinating disorders. These findings suggest a premyelinating function of the PLP gene products. We previously demonstrated that PLP gene expression is directly associated with secretion of a factor that increases the number of oligodendrocytes. Here we show that this activity is mediated by a secreted fragment containing the C-terminal portion of PLP. This factor increased the bromodeoxyuridine incorporation rate in both oligodendrocyte and astrocyte lineage cells; a synthetic peptide (PLP 215-232) exhibited a similar activity. Dose-response curves of PLP and PLP peptide showed maximum activities at a concentration in the picomolar range, which decreased at higher concentrations. These observations demonstrate that a secreted PLP gene product exerts biological activity at a premyelinating stage before the major induction of the gene.  (+info)

Embryonic and postnatal injections of bromodeoxyuridine produce age-dependent morphological and behavioral abnormalities. (6/3497)

The mitotic marker 5-bromodeoxyuridine (BrdU) was injected twice daily (60 mg/kg) into pregnant hooded rats on one of embryonic days (E) 11, 12, 13, 15, 17, or 21, or into rat pups on postnatal day (P) 10. The principal findings were the following: (1) BrdU exposure on E11 produces profound effects on body morphology, and animals must be fed a special diet because of chronic tooth abnormalities; (2) BrdU exposure at E17 or earlier produces a change in coat spotting pattern, the precise pattern varying with age; (3) BrdU exposure on E15 or earlier produces a reduction in both brain and body weight; (4) BrdU exposure on E17 or earlier reduces cortical thickness; (5) BrdU exposure on E11-E13 and at P10 reduces cerebellar size relative to cerebral size; (6) spatial learning is significantly affected after injections of BrdU at E11-E17, but the largest effect is on E17; (7) the deficit in spatial learning may be related in part to a reduction in visual acuity; and (8) skilled forelimb ability is most disrupted after BrdU exposure at E15 but is also impaired after injections on E13 or earlier. BrdU thus has teratological effects on body, brain, and behavior that vary with the developmental age of the fetus or infant.  (+info)

Capsaicin-sensitive C-fiber-mediated protective responses in ozone inhalation in rats. (7/3497)

To assess the role of lung sensory C fibers during and after inhalation of 1 part/million ozone for 8 h, we compared breathing pattern responses and epithelial injury-inflammation-repair in rats depleted of C fibers by systemic administration of capsaicin as neonates and in vehicle-treated control animals. Capsaicin-treated rats did not develop ozone-induced rapid, shallow breathing. Capsaicin-treated rats showed more severe necrosis in the nasal cavity and greater inflammation throughout the respiratory tract than did control rats exposed to ozone. Incorporation of 5-bromo-2'-deoxyuridine (a marker of DNA synthesis associated with proliferation) into terminal bronchiolar epithelial cells was not significantly affected by capsaicin treatment in rats exposed to ozone. However, when normalized to the degree of epithelial necrosis present in each rat studied, there was less 5-bromo-2'-deoxyuridine labeling in the terminal bronchioles of capsaicin-treated rats. These observations suggest that the ozone-induced release of neuropeptides does not measurably contribute to airway inflammation but may play a role in modulating basal and reparative airway epithelial cell proliferation.  (+info)

Retinal neurogenesis: the formation of the initial central patch of postmitotic cells. (8/3497)

We have investigated the relationship between the birthdate and the onset of differentiation of neurons in the embryonic zebrafish neural retina. Birthdates were established by a single injection of bromodeoxyuridine into embryos of closely spaced ages. Differentiation was revealed in the same embryos with a neuron-specific antibody, zn12. The first bromodeoxyuridine-negative (postmitotic) cells occupied the ganglion cell layer of ventronasal retina, where they formed a small cluster of 10 cells or less that included the first zn12-positive cells (neurons). New cells were recruited to both populations (bromodeoxyuridine-negative and zn12-positive) along the same front, similar to the unfolding of a fan, to produce a circular central patch of hundreds of cells in the ganglion cell layer about 9 h later. Thus the formation of this central patch, previously considered as the start of retinal neurogenesis, was actually a secondary event, with a developmental history of its own. The first neurons outside the ganglion cell layer also appeared in ventronasal retina, indicating that the ventronasal region was the site of initiation of all retinal neurogenesis. Within a column (a small cluster of neuroepithelial cells), postmitotic cells appeared first in the ganglion cell layer, then the inner nuclear layer, and then the outer nuclear layer, so cell birthday and cell fate were correlated within a column. The terminal mitoses occurred in three bursts separated by two 10-h intervals during which proliferation continued without terminal mitoses.  (+info)

Detect BrdU also known as Bromodeoxyuridine with Anti-BrdU Antibody, clone BU-1 (Mouse Monoclonal Antibody), that has been demonstrated to work in ICC. Find MSDS or SDS, a COA, data sheets and more information.
PromoCells Cell Proliferation Assay Kit II (BrdU) detects incorporated BrdU using a mouse anti-BrdU antibody. An anti-mouse HRP-linked secondary antibody is used to detect the anti-BrdU antibody bound to BrdU, which is followed by addition of TMB (a HRP substrate). The extent of color development is proportional to the quantity of BrdU incorporated into the cells and can be used directly as an indicator of cell proliferation. Compared to other cell proliferation assays, this kit detects only the proliferating cells and not the seeded cells. This highly sensitive, non-radioactive kit detects as less as 50-100 proliferating cells. ...
Chinese hamster ovary cells were seeded in the absence or presence of the polyamine synthesis inhibitor 2-difluoromethylornithine (DFMO). At 1-4 days after seeding, the cells were labelled for 15-120 min with the thymidine analogue bromodeoxyuridine (BrdUrd) and they were then fixed directly after the labelling period. In addition, cells were labelled for 30 min and they were then allowed to progress in BrdUrd-free medium during a defined post-labelling time before fixation. An indirect immunofluorescence technique, using the monoclonal BrdUrd antibody and the intercalating stochiometric DNA stain, propidium iodide, was applied to enable quantification of cellular BrdUrd and DNA contents, respectively, by flow cytometry (FCM). By comparing the mean DNA content of BrdUrd-labelled cells to the mean DNA contents of G(1) and G(2) cells, a relative measure of the position of the BrdUrd-labelled cells was obtained (relative movement). Relative movement data, obtained from control and DFMO-treated ...
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DiviTum™ assay determines the enzymatic activity of TK in patient samples. During the assay procedure, thymidine is replaced by its synthetic analog bromodeoxyuridine (BrdU), which gets phosphorylated and then incorporated into a synthetic DNA strand fixated in each well of a 96-well ELISA immunosorbent titer-plate.. The extent of BrdU incorporation depends on the activity of TK present in the serum sample; the more TK activity in the sample, the more BrdU is incorporated into synthetic DNA strands in the titer-plate well. The synthetic BrdU is then detected with anti-BrdU specific antibodies using the well-known ELISA assay technique. The DiviTum™ technology amplifies the signal, enabling the assay to measure thymidine kinase activity with high sensitivity.. ...
Abstract The interaction of a nucleoside analogue bromodeoxyuridine (BrdU) with human serum albumin (HSA) was studied to investigate the binding phenomenon and analyse the protein conformation upon...
Mouse anti-BrdU antibody, clone Bu20a recognizes incorporated BrdU in a variety of cell types. It is suitable for use on tissue sections in double-labeling techniques.
Anti-BrdU monoclonal antibody is offered as a stand alone product, for use in labeling cells that have incorporated BrdU (cells incubated in the presence of BrdU will incorporate this thymidine analog into any DNA that is synthesized during that time). This antibody, from the PRB-1 clone ... ...
Monoclonal antibody against 5-bromo-2-deoxyuridine BrdU expressed by BrdU for use in FACS, FFPE, Immunofluorescence, Immunohistochemistry against All species labeled with BrdU
We have brought together our full range of anti-BrdU antibodies in one place, making it easy for you to find exactly what you need. Buy online today
TY - JOUR. T1 - Increased incorporation of 5-bromodeoxyuridine into the DNA of proliferating tissues in partially hepatectomised mice. AU - Hill, Bridget T.. AU - Augenlicht, Leonard H.. AU - Baserga, Renato. PY - 1973/12/1. Y1 - 1973/12/1. UR - http://www.scopus.com/inward/record.url?scp=0015897107&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0015897107&partnerID=8YFLogxK. U2 - 10.1016/0014-5793(73)80482-X. DO - 10.1016/0014-5793(73)80482-X. M3 - Article. C2 - 4763337. AN - SCOPUS:0015897107. VL - 37. SP - 298. EP - 302. JO - FEBS Letters. JF - FEBS Letters. SN - 0014-5793. IS - 2. ER - ...
Return to Modified Bases Modifications 5-Bromo-deoxyuridine is a photoreactive halogenated base that can be incorporated into oligonucleotides to crosslink them to DNA, RNA or proteins with exposure to UV light. Crosslinking is maximally efficient with light at 308 nm.. ...
Mouse genotyping. Null and wild-type mice were obtained from heterozygous p27Kip1 breeding pairs (in a mixed genetic background of C57BL/6 and B6SJL) (Kiyokawa et al., 1996). The genotype was determined by PCR analysis of genomic tail DNA. Briefly, tails were digested in lysis buffer (100 mm NaCl, 10 mm Tris, pH 8.0, 0.5% SDS, 25 mm EDTA, 148 μg/ml proteinase K stock) for 14-18 hr at 55°C. Genomic DNA was isolated and amplified by PCR using the following primers: 5′-CGCCCCGACTGCATCTGCGTGTTCGAA-3′ and 5′-TCAAACGTGAGAGTGTCTAACGG-3′ directed against thep27Kip1 gene and 5′-AGGGCTTATGATTCTGAAAGTCG-3′ corresponding to the neo sequence. After a 35 cycle reaction (denaturation at 94°C for 45 sec, annealing at 62°C for 1 min, extension at 72°C for 1 min), the wild-type allele yielded a 210 bp PCR product and the null allele yielded a longer 290 bp product because of neo insertion in the first exon.. Whole-mount bromodeoxyuridine incorporation.Bromodeoxyuridine (BrdU, Sigma, St. Louis, ...
Buy our Pescadillo 293T transfected lysate (positive control). ab94282 has been validated in western blot. Abcam now offers a 12-month guarantee.
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Mouse Monoclonal Anti-Bromodeoxyuridine/BrdU Antibody (BU20a) [DyLight 550]. Proliferation Marker. Validated: Flow, ICC/IF, IHC-Fr, IHC-P. Tested Reactivity: All Species. 100% Guaranteed.
Mouse Monoclonal Anti-Bromodeoxyuridine/BrdU Antibody (BU20a) [DyLight 488]. Proliferation Marker. Validated: Flow, ICC/IF, IHC-Fr, IHC-P. Tested Reactivity: All Species. 100% Guaranteed.
Analysis of turnover of Treg by BrdU incorporation. BALB/c mice were treated with BrdU administered continuously for 7 d using osmotic pumps. Then, peripheral L
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Under normal conditions, the adult zebrafish telencephalic VZ continuously supplies new neurons to the OB (Byrd and Brunjes, 2001; Grandel et al., 2006; Adolf et al., 2006; Kishimoto et al., 2011). We next performed a BrdU pulse-chase experiment to trace the migrating newly born progeny of ventricular proliferative cells from the telencephalic VZ to the injury site (Zupanc et al., 2005; Grandel et al., 2006; Adolf et al., 2006; Kishimoto et al., 2011). We followed the BrdU-labeled cells in three telencephalic regions - the VZ, the subpallium and the mdlPa (the region surrounding the injury site) - on 0, 3, 7, 10 and 14 dpl (Fig. 4A). At 0 dpl, a large number of BrdU-labeled cells was detected in the telencephalic VZ (n=5; Fig. 4B,B′,F). At 3 dpl, BrdU-labeled cells appeared in the subpallium and pallium along the pathway connecting the telencephalic VZ and the injury site in the injured hemisphere (n=5; Fig. 4C,C′,G). From 3 dpl onwards, the number of BrdU-labeled cells decreased in the ...
Under normal conditions, the adult zebrafish telencephalic VZ continuously supplies new neurons to the OB (Byrd and Brunjes, 2001; Grandel et al., 2006; Adolf et al., 2006; Kishimoto et al., 2011). We next performed a BrdU pulse-chase experiment to trace the migrating newly born progeny of ventricular proliferative cells from the telencephalic VZ to the injury site (Zupanc et al., 2005; Grandel et al., 2006; Adolf et al., 2006; Kishimoto et al., 2011). We followed the BrdU-labeled cells in three telencephalic regions - the VZ, the subpallium and the mdlPa (the region surrounding the injury site) - on 0, 3, 7, 10 and 14 dpl (Fig. 4A). At 0 dpl, a large number of BrdU-labeled cells was detected in the telencephalic VZ (n=5; Fig. 4B,B′,F). At 3 dpl, BrdU-labeled cells appeared in the subpallium and pallium along the pathway connecting the telencephalic VZ and the injury site in the injured hemisphere (n=5; Fig. 4C,C′,G). From 3 dpl onwards, the number of BrdU-labeled cells decreased in the ...
国内在庫あります!Biotin標識済みシープ・ポリクローナル抗体 ab2284 適用: IP,ELISA,IHC-FoFr,IHC-P,IHC-Fr,ICC/IF…BrdU抗体一覧 一次抗体にBiotinを直接標識し、操作時間の短縮と低いバックグラウンドを実現。
Bromodeoxyuridine (BrdU) (Proliferation Marker) Antibody - With BSA and Azide, Mouse Monoclonal Antibody [Clone BRD469 + BRD494 + BRD.3 ] validated in IHC-P, IF, FC (AH10977-20), Abgent
Now i have a new problem! This BrdU protocol needs Denaturation. Now i want to monitor GFP-transfected cells too. The HCl seems to denature the endogeneous GFP too. Is there a more acid resistable GFP? Perhaps an anti-GFP antibody? Or may i choose another method to denaturize the DNA for the anti-BrdU? Maybe temp.?Or stain for myc-tagged GFP ...
Monoclonal Anti-Bromodeoxyuridine (Clone BU6-4) is often used in cell cycle analysis studies; the level of bromodeoxyuridine (BrdU) incorporation into DNA is a direct parameter of cell number and growth. The monoclonal anti-bromodeoxyuridine antibody can be used in Western blot analysis under non-reducing and non-heating conditions and in flow cytometry applications. ...
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Because our examination of cell death did not reveal a loss of newly generated neurons in the cortical plate, we asked whether the reduced neuronal numbers in p107-deficient mice was caused by fewer neurons born at E13.5 (the time of BrdU injection). To address this question, we performed a 24-h BrdU incorporation to measure the rate of neuronal commitment. Pregnant dams were injected at E13.5, embryos were collected 24 h later at E14.5, and the number of strongly labeled BrdU-positive cells were counted (i.e., cells that underwent terminal mitosis at the time of injection). In addition, sections were double stained with PCNA to show that these cells are no longer cycling. Double labeling with BrdU and PCNA revealed that most BrdU-positive cells within the SVZ and IZ were no longer expressing PCNA, indicating that they were newly postmitotic (Fig. 8, d-f). Cell counts of this newly postmitotic population revealed a two-fold reduction in p107-deficient brains compared with wild-type controls ...
Mice exposed to the OIR model were injected with 0.1 mg/g 5-bromo-2-deoxyuridine or 5-ethynyl-2-deoxyuridine (EdU) dissolved in sterile water 21 and were humanely killed 2 hours after injection. For BrdU, the eyes were fixed in 4% paraformaldehyde (PFA) on ice for 3 minutes, then transferred to 70% ethanol and stored at −20°C for 2 hours. They were then washed in decreasing concentrations of ethanol for 10 minutes each, dissected in PBS, and washed for 30 minutes in 1 mL PBS/1% Triton X-100 on a horizontal shaker at low speed. The retinas were incubated at 37°C for 1 hour with 2 N HCl, washed in 1 mL 0.1 M sodium borate twice for 15 minutes, incubated overnight at 4°C with biotin-conjugated anti-BrdU antibody diluted 1:300 in PBS containing 1% BSA, and then washed three times with PBS-1% Triton X-100 for 5 minutes while being shaken at low speed. Retinas were incubated in Texas Red-labeled anti-biotin diluted 1:500 in PBS, 1% BSA for 2 hours with shaking while shielded from light, then ...
As the previously described method [4,22], 5-bromo-2-deoxyuridine (BrdU) immunohistochemistry was performed. The brain sections were treated with 0.5% Trioton X-100 in PBS for 20 minutes, treated with 50% formamide-2 x standard saline citrate at 65℃ for 2 hours, treated with 2 N HCl at 37℃ for 30 minutes, and then treated with 100mM sodium borate (pH, 8.5). The sections were treated with mouse monoclonal anti-BrdU antibody (1:600; Roche) during overnight at 4℃, treated with biotionylated mouse secondary antibody (1:200; Vector Laboratories, Burlingame, CA, USA) for 90 minutes, and treated with avidin-peroxidase complex (1:100; Vector Laboratories). To visualize, the section was treated with 50mM Tris-HC1 (pH, 7.6) in 0.02% DAB, 40-mg/mL nickel chloride, and 0.03% H2O2 during 5 minutes. With a mouse monoclonal anti-neuronal nucleic antibody (1:300; Chemicon International, Temecula, CA, USA), counter-staining was conducted upon the same sections. After the slides were dried under the room ...
Purpose: To examine the anatomical localization of limbal T cells relative to label-retaining cells (LRCs). Previously, we demonstrated the presence of presumed limbal regulatory (Treg) Foxp3-GFP+ T cells via in vivo epifluorescent imaging. In this study, we further characterize the localization of T cells in the limbus in conjunction with LRCs, i.e. presumed limbal stem cells, via immunofluorescence tomography.. Methods: H2B-GFP/K5tTA (HGK) mice express histone H2B-green fluorescent protein (GFP) under the control of the tetracycline and keratin 5 promoters, resulting in retention of GFP in slow-cycling LRCs after doxycycline administration. Following a 16 week pulse-chase period, the limbal region of HGK mice was fixed with 2% paraformaldehyde, sectioned and embedded in butyl-methyl-methacrylate, subjected to immunohistochemistry (IHC) with the T cell marker CD3, and imaged via immunofluorescence tomography to create high-resolution 3-D reconstructions of 200 sections. 3-D reconstructions were ...
Overall, our 2H2O labeling results are consistent with measurements of hippocampal neurogenesis by BrdU labeling. Assuming that the hippocampus has ≈2 × 106 total cells (Abusaad et al., 1999), the labeling rate of 0.2% per day in total C57Bl/6 hippocampus that we observed with 2H2O would be equivalent to ≈4000 labeled cells/day, a value comparable with estimates by 12- to 24-h saturation labeling with BrdU (Hayes and Nowakowski, 2002). Palmer et al. (1999) reported that 0.7% of progenitor cells were labeled after daily BrdU injection for 6 days in a confocal analysis of BrdU incorporation into gradient-isolated progenitor cells from rat hippocampus, a similar value as we measured by 2H2O labeling on similarly isolated rat hippocampal progenitors (1% per week). In mice, the hierarchy of baseline progenitor cell proliferation across different mouse strains in our study agreed well with strain effects on total hippocampal cell proliferation obtained by BrdU labeling (C57Bl/6 , BALB/c , ...
One of the major drawbacks of droplet sorting in a flow cytometer is the relatively low sorting speed. Thus, we have developed an alternative, faster sorting technique: photodamage cell sorting. In a photodamage cell sorter all unwanted cells, as detected with the first, measuring laser, are killed with the second, damaging laser. Thus, the cells need to be photosensitive to the second laser. In addition, a mechanism is needed to switch this laser on and off based on the sorting criteria. In our photodamage cell sorter, the ZAPPER, we use an acousto-optic crystal to switch the laser beam. Cells are made photosensitive by vital staining with photosensitizers. With cells grown in the presence of 5-bromo-2′-deoxyuridine (BrdUrd) and stained with Hoechst 33342 (H42) at least a 5-decade cell reduction is accomplished after irradiation with 400 mW UV light. With this system, sorting rates have been achieved of 30,000 cells per second. Due to the selection based on photodynamic killing, this sorting ...
Results: This study reports the eventual outcome of the 97 patients after 12 years. There were no significant associations between proliferation data of the index tumours and patient outcome. No adverse events were identified which could be attributed to the use of the halogenated pyrimidine label in vivo ...
Microfiche of typescript. [Urbana, Ill.]: Photographic Services, University of Illinois, U of I Library, [1989]. 2 microfiches (41 frames): negative ...
We have studied the effect of prenatal (late intrauterine) 5-bromo-2-deoxyuridine (BrdU) administration on the development of the GnRH pathway. BrdU is widely accepted as a tool for studying neurogenesis (pre and postnatal). On the other hand, this thymidine analogue proved to be effective influencing different developmental events too. Dams of C57Bl6 mice were treated on the last days of pregnancy (between E16, E17, E18, E19 and the time of birth, E20/21 days, respectively). The dams received daily injections of 15 ug/g body weight BrdU subcutaneously, dissolved in physiological saline, between 11 and 12 am. BrdU treatment during the last 3-4 days of pregnancy resulted in a significant decrease of the number of GnRH immunoreactive (ir.) neuronal cell bodies as well as ir. fibers. This decrease could be detected at all postnatal ages (P0/P1, P3/4, P6/8 and P21/23) studied. The higher cumulative doses of BrdU produced a more significant decrease of the GnRH ir. structures in comparison with the ...
symbol: BrUra; 5‐bromouracil; 5‐bromo‐2,4(1H,3H)‐pyrimidine‐dione; a synthetic analogue of thymine with mutagenic activity. It is incorporated into DNA as bromodeoxyuridine, which replaces thymidine and induces transitions of G‐C base‐pairing to A‐T ... ...
We report a novel mechanism of cellular growth control. Increasing the density of endothelial or smooth muscle cells in culture increased cell-cell contact and decreased cell spreading, leading to growth arrest. Using a new method to independently control cell-cell contact and cell spreading, we fou …
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Several types of assays can be performed measuring galactosidase activity in yeast using 5-Bromo-4-chloro-3-indoxyl-α-D-fucopyranoside as subtrate.
Kent. D. Dunlap, Anna C. Silva and Michael Chung. Environmental complexity, seasonality and brain cell proliferation in a weakly electric fish, Brachyhypopomus gauderio. J exp Biol 2011; 214, 794-805 Full Text IH. Pytte CL, Parent C, Wildstein S, Varghese C, Oberlander S. Deafening decreases neuronal incorporation in the zebra finch caudomedial nidopallium (NCM). Behav Brain Res. 2010 Aug 25;211(2):141-7 Full Text IH. Lassmann J, Sliwoski J, Chang A, Canning DA, Zderic SA. Deletion of One SERCA2 Allele Confers Protection Against Bladder Wall Hypertrophy in a Murine Model of Partial Bladder Outlet Obstruction. Am J Physiol Regul Integr Comp Physiol. 2008 Jan;R58-65 Full Text ...
TY - JOUR. T1 - Wound splinting modulates granulation tissue proliferation. AU - Carlson, Mark Alan. AU - Thompson, Jon S. PY - 2004/7/1. Y1 - 2004/7/1. N2 - Attachment of the extracellular matrix to a substratum is important for fibroblast survival and proliferation in three-dimensional in vitro culture systems. We hypothesized that wound matrix attachment in a wound splinting model would modulate wound cell proliferation in vivo. Male rats were excisionally wounded on the dorsum, and a splint was sutured to the wound edge. In one experiment (N=12), 6 rats were desplinted on day 5, and then all were sacrificed 24 h later, 6 h after 5-bromo-2′-deoxyuridine (BrdU) injection. In the second experiment (N=18), 6 rats each were desplinted, desplinted with wound edge release, or not disturbed, followed by BrdU injection and sacrifice 24 h later. BrdU-labeled nuclei were quantified on frozen sections of granulation tissue, cut at three different levels. In the first experiment, the percentage of ...
Subventricular Zone-Derived Neural Progenitor Cells Migrate Along a Blood Vessel Scaffold Toward the Post-Stroke Striatum Kojima T, Hirota Y, Ema M, Takahashi S, Miyoshi I, Okano H, Sawamoto K.. The subventricular zone (SVZ) of the adult brain contains neural stem cells that have the capacity to regenerate new neurons after various insults. Brain ischemia causes damage to brain tissue and induces neural regeneration together with angiogenesis. We previously reported that, after ischemic injury in mice, SVZ-derived neural progenitor cells (NPCs) migrate into the striatum, and these NPCs are frequently associated with blood vessels in the regenerating brain tissue. Here we studied the role of blood vessels during the neural regeneration in more detail. BrdU administration experiments revealed that newly generated NPCs were associated with both newly formed and pre-existing blood vessels in the ischemic striatum, suggesting that the angiogenic environment is not essential for the ...
This study demonstrates a subset of murine mammary epithelial cells that is long label retaining and expresses ER-α or the PR. Previously, it was shown that more than 80% of LRECs are actively traversing the cell cycle, despite retention of the original DNA label [4]. The retention of the original DNA label over many weeks serves to establish the validity of our conclusion that the LRECs represent long-lived cells. Whether these label-retaining cells remain ER-α and/or PR positive from their inception through the entire chase period is not possible to determine. Nevertheless, we observed a high percentage of ER-α and PR positive, [3H]thymidine labeled, epithelial cells following the original labeling period. In addition, we did not establish whether ER-α and PR are simultaneously expressed in these cells. Recent data indicate that the expression of active transforming growth factor (TGF)-β1 restrains ER-α-positive cells among the mouse mammary epithelium from entering the cell cycle [6]. ...
Bromodeoxyuridine labeling study may be helpful in the diagnosis of meningioma. An elevated bromodeoxyuridine labeling index is suggestive of a rapid growth rate of meningioma and a greater incidence of recurrence following surgical resection. ...
To determine the effect of protein treatments in cell proliferation, MDA-MB-231 breast cancer cells were first injected into the mammary fat pad of nude mice, and when tumors grew to 10 mm in diameter, mice were treated once only with purified Endo (20 mg/kg), CD (40 mg/kg), or EndoCD (60 mg/kg) proteins plus 500 mg/kg 5-FC, a clinically sufficient dose of 5-FU (15 mg/kg; 1× 5-FU), or 10 times the clinically sufficient dose (150 mg/kg; 10× 5-FU). The choice of 20 mg/kg Endo was based on a previous preclinical study (15) and is also within the dose tested in the phase I clinical trial (ref. 18; 15-600 mg/m2 in human is equivalent to 4.8-194.4 mg/kg in mouse; ref. 30). Tumors were harvested from mice 48 hours after treatment and labeled with bromodeoxyuridine (BrdU) antibody for in vivo BrdU incorporation analysis. The results show that EndoCD/5-FC most significantly reduced cancer cell proliferation (Fig. 2B) compared with all other treatment groups.. The potent inhibitory activity of ...
Immunohistochemical detection of cells labeled with bromodeoxyuridine after neural transplantation.: Pregnant rats were treated with 5-bromo-2-deoxyuridine (Br
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Fish and also neonatal mice possess the capacity to regenerate their hearts after myocardial damage. The adult mammalian heart only has a very limited capacity to regenerate after cardiac injury. Myocardial regeneration in zebrafish and newborn mice involves the dedifferentiation and proliferation of resident cardiomyocytes to replace the injured tissue. Unfortunately, only little is known about the factors and signaling cascades that regulate this regenerative capacity.. In a forward genetics ENU-screen in zebrafish, we recently isolated the mutant heart of stone (hos). Hearts of hos mutant embryos show a severe thickening of the ventricle with almost complete obliteration of the ventricle. Based on cell count assays using histological sections we find significantly increased cell numbers in hos mutant ventricles. Based on BrdU incorporation assays as well as H3P and PCNA stainings, we find that ventricular hyperplasia in hos is caused by significantly enhanced cardiomyocyte ...
Supplementary MaterialsSupplementary Components: Supplementary desk 1: major antibodies tested within this research. organs share commonalities in physiology, mobile size and composition to individuals. As a result, characterization of stem/progenitor cells in porcine organs or tissue might provide a book avenue to raised understand the biology and function of stem cells in human beings. In todays research, potential stem/progenitor cells in performing airway epithelium of the porcine lung had been seen as a morphometric evaluation of bromodeoxyuridine (BrdU) label-retaining cells (LRCs) through the maturation from the lung. The full total outcomes demonstrated a pseudostratified mucociliary epithelium made up of basal, ciliated, goblet, and columnar cells in the performing airway of the porcine lung. Furthermore, nearly all major epithelial cells in a position to proliferate in vitro portrayed keratin 5, a subpopulation of the keratin 5-positive cells, also portrayed Compact disc117 (c-Kit) or ...
Many adult regenerative cells divide infrequently but have high proliferative capacity. We developed a strategy to fluorescently label slow-cycling cells in a cell type-specific fashion. We used this method to purify the label-retaining cells (LRCs) that mark the skin stem cell (SC) niche. We found that these cells rarely divide within their niche but change properties abruptly when stimulated to exit. We determined their transcriptional profile, which, when compared to progeny and other SCs, defines the niche. Many of the ,100 messenger RNAs preferentially expressed in the niche encode surface receptors and secreted proteins, enabling LRCs to signal and respond to their environment.. ...
The recent advancement of targeted murine reporter alleles as proxies for intestinal stem cell activity has led to significant advances in our understanding of somatic stem cell hierarchies and mechanics. a paradigmatic model for understanding come cell business and mechanics in extremely proliferative cells. The past 10 years offers noticed ABR-215062 ABR-215062 several discoveries in our understanding of digestive tract come cells (ISCs). To 2007 Prior, the presence of ISCs at the foundation of little digestive tract crypts was a subject matter of rumours. Undifferentiated, radiosensitive label-retaining cells (LRCs) around the?+4 position from the crypt foundation experienced lengthy been postulated to become ISCs (Potten et?al., 2002); nevertheless, no practical data confirming the developing capability of these cells been around. Starting in 2007, a series of milestone research recognized many loci that designated practical digestive tract come cells upon attachment of an inducible Cre ...
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Two compounds, bromovinyldeoxyuridine ([E]-5-[2-bromovinyl]-2-deoxyuridine) and trifluridine (5-trifluoromethyl-2 deoxyuridine) were compared for their efficacy in the topical treatment of experimental stroma herpetic keratitis produced by the injection of live herpes simplex virus, type 1, into the corneal stroma of rabbits. The trifluridine was used as 1% eyedrops, whereas bromovinyldeoxyuridine was used as either 0.1% or 0.5% eyedrops. All three treatment regimens caused a substantial healing of stroma disease in comparison with placebo treatment. The bromovinyldeoxyuridine (whether used as 0.1% or 0.5%) proved superior to 1% trifluridine eyedrops when the treatment was started one day after virus inoculation. However, bromovinyldeoxyuridine and trifluridine were equally effective if the treatment was started seven days after infection. The 0.5% bromovinyldeoxyuridine eyedrops seemed to be more effective in controlling the complicating severe iritis and secondary glaucoma than either 0.1% ...
The aim of this study was to investigate the effect of antenatal glucocorticoid therapy on smooth-muscle-cell (SMC) DNA synthesis in the pulmonary arteries (PA) in a nitrofen-induced congenital diaphragmatic hernia (CDH) rat model following nitrofen administration on day 9.5 of gestation. Antenatal dexamethasone (DEX) was given intraperitoneally on days 18.5 and 19.5 of gestation. Bromodeoxyuridine (BrdU) was injected via a jugular vein into the dam 1 h before the fetuses were killed by cesarean section at term. The fetuses were divided into three groups: group I (n = 10): normal controls; group II (n = 10): nitrofen-induced CDH; group III (n = 10): nitrofen-induced CDH with antenatal DEX treatment. Immunostaining of the lungs with anti-BrdU antibody was obtained by a standard avidin-biotin complex method. The number of immunopositive cells in the PA media and adventitia were counted using an image analyzer and analyzed statistically. The number of BrdU-immunopositive cells in the media was ...
Harold M. "Hal" Weintraub was an American scientist who lived from 1945 until his death in 1995 from an aggressive brain tumor. Only 49 years old, Weintraub left behind a legacy of research. Born on June 2, 1945 in Newark, New Jersey, Weintraubs childhood revolved around sports, including basketball, an activity he would continue to particularly relish throughout his adult life. Weintraub was also the pitcher for an all-city high school baseball team, and a football fullback. Weintraub attended Harvard College, obtaining his bachelors degree in 1967. He then proceeded to the University of Pennsylvania, where he earned his M.D. and Ph.D. in 1972. Weintraub performed his Ph.D. dissertation research in the laboratory of Howard Holtzer, studying red blood cell development and production (erythropoeisis) in chicken embryos. This work included the study of cell cycle kinetics, hemoglobin synthesis, and the control of cell division. The effects of bromodeoxyuridine on cell differentiation (conversion ...
Inhibition of phosphodiesterase-4 (PDE4), an enzyme that catalyzes the hydrolysis of cyclic AMP (cAMP), increases phosphorylation of the cAMP response element binding protein (pCREB) and hippocampal neurogenesis, and produces antidepressant-like effects on behavior; however, causal links among these actions have not been established. In this study, chronic administration of rolipram (0.31-1.25 mg/kg, 16-23 days) produced antidepressant- and anxiolytic-like effects on behavior in mice. It also increased cAMP and pCREB levels in the hippocampus and prefrontal cortex, but increased Sox2, a marker for mitotic progenitor cells, only in the hippocampus. Chronic rolipram treatment also increased hippocampal neurogenesis, as evidenced by increased bromodeoxyuridine (BrdU)-positive cells in the hippocampal dentate gyrus. Methylazoxymethanol (MAM), which is toxic to proliferating cells, reversed rolipram-induced increases in BrdU-positive cells and pCREB in the hippocampus and partially blocked its ...
The Wnt signaling pathway plays multiple roles during adult neurogenesis, regulating proliferation, differentiation and maturation of newborn neurons [15-18, 40]. Here we studied the potential role of the Wnt receptor FZD1 during adult hippocampal neurogenesis. We specifically focused on FZD1 since it is expressed in the adult hippocampus [22, 25], and is well known to activate the canonical Wnt/β-catenin signaling pathway [25-29]. We found that in the adult dentate gyrus, FZD1 is mainly expressed in NSCs, amplifying progenitors and immature neurons, suggesting that FZD1 may mediate the activation of the Wnt pathway in these cell-types. In agreement, the expression of FZD1 was also found in cultured AHPs that were isolated from hippocampi of adult mice.. The in vivo role of FZD1 in adult neurogenesis was evaluated by retrovirus-mediated shRNA knockdown of FZD1 expression in newborn cells of the dentate gyrus. We determined that differentiation into neurons was impaired in FZD1-deficient cells. ...
In the stab-injured spinal cord of GFAP-TK mice not given GCV, we found that all TK-expressing cells also expressed GFAP, and that ,98%, but not all, of GFAP-expressing reactive astrocytes also expressed detectable levels of transgene-derived TK (Fig. 2A-D,G). The inability to detect TK in all GFAP-expressing cells may be attributable to the discontinuous activity of the GFAP gene and differences in the intracellular half-life of the GFAP and TK proteins, as discussed elsewhere (Bush et al., 1999; Imura et al., 2003). We found no evidence for TK expression by cells that did not express GFAP.. To evaluate the degree of astrocyte cell division induced by SCI, we administered BrdU during the first 4 d after injury (Fig. 1A). At 14 d after injury, control mice exhibited many BrdU-labeled cells in tissue immediately adjacent to the stab wound (Fig. 2E,F); most of these BrdU-labeled cells expressed GFAP (Fig. 2H) and TK.. There was no qualitatively detectable difference in the response to SCI in all ...
Growth of HSV-1 in (E)-5-(2-bromovinyl)-2-deoxyuridine (BVDU) results in incorporation of the analog into HSV-1 DNA. Employing the technique of Weigle-type reactivation (WR), HSV-1 inactivated by uv irradiation but not by growth in BVDU is a substrate for induced cellular repair pathways. Growth of mammalian cells in BVDU does not induce cellular repair pathways detected by WR. HSV-1 temperature sensitive (ts) mutants demonstrate an increased reversion frequency to the nonpermissive phenotype (ts+) following growth in a high concentration of BVDU. A ts mutant did not display an increased reversion frequency following growth in equally inhibitory concentrations of an HSV-1 polymerase inhibitor, aphidicolin. These findings suggest that BVDU incorporated into HSV-1 DNA may not be readily excised and may be a mutagenic lesion in viral DNA, although other explanations are possible ...
Introduction: Antiproliferative effects of immunosuppressants used in human islet transplantation interfere with the capability of the beta cells to balance cell renewal and cell loss. Consequently, long-term use of these drugs might contribute to graft dysfunction in islet transplant recipients. New immunosuppressive regimens are required to improve outcomes.. Materials and methods: Syngeneic islets (300 IEQ) were injected into the right liver lobes of C57BL/6 diabetic mice. Osmotic pumps filled with Bromodeoxyuridine (group 1), Bromodeoxyuridine and Tacrolimus (group 2) or Bromodeoxyuridine and Everolimus (group 3) were implanted. Hepatectomy was performed after 4 weeks. Proliferation of beta cells was detected by BrdU incorporation. Results: In all transplanted animals normoglycemia was restored. Glucose tolerance was significantly improved after 4 weeks in group 1 (90min: P, 0.012; 120min: P,0.045). This effect was not as strong when animals were treated with Tacrolimus. In contrast, mice ...
Human JC polyomavirus (JCV) causes progressive multifocal leukoencephalopathy (PML), a rare but life-threatening demyelinating disease in patients under profound immunosuppression and those receiving therapeutic immunomodulation for autoimmune and inflammatory disorders. Using persistent mouse polyomavirus (MPyV) infection in mice, we are developing a model of PyV-induced CNS disease to elucidate the role of immunosuppression in PML pathogenesis. Histology revealed corpus callosum demyelination in 100% of persistently infected mice. Following intracerebral injection with MPyV, immunocompetent C57BL/6 mice showed robust infiltration by MPyV-specific CD8 T cells with minimal contraction. During persistent CNS infection, a significant population of CD8 T cells exhibited a tissue-resident memory (Trm) phenotype (CD103+CD69+CD62Llo), as reported for acute VSV and latent HSV-1 infections, and incorporated BrdU in situ, suggesting capacity for self-renewal. Furthermore, these Trm cells develop and ...
TY - JOUR. T1 - Radiation-induced impairment of hippocampal neurogenesis is associated with cognitive deficits in young mice. AU - Rola, Radoslaw. AU - Raber, Jacob. AU - Rizk, Angela. AU - Otsuka, Shinji. AU - Vandenberg, Scott R.. AU - Morhardt, Duncan R.. AU - Fike, John R.. PY - 2004/8. Y1 - 2004/8. N2 - Advances in the management of pediatric brain tumors have increased survival rates in children, but their quality of life is impaired due to cognitive deficits that arise from irradiation. The pathogenesis of these deficits remains unknown, but may involve reduced neurogenesis within the hippocampus. To determine the acute radiosensitivity of the dentate subgranular zone (SGZ), 21-day-old C57BL/J6 male mice received whole brain irradiation (2-10 Gy), and 48 h later, tissue was assessed using immunohistochemistry. Proliferating SGZ cells and their progeny, immature neurons, were decreased in a dose-dependent fashion. To determine if acute changes translated into long-term alterations in ...
When DArcy Wentworth Thompsons On Growth and Form was published 100 years ago, it raised the question of how biological forms arise during development and across evolution. In light of the advances in molecular and cellular biology since then, a succinct modern view of the question states: how do genes encode geometry? Our new special issue is packed with articles that use mathematical and physical approaches to gain insights into cell and tissue patterning, morphogenesis and dynamics, and that provide a physical framework to capture these processes operating across scales.. Read the Editorial by guest editors Thomas Lecuit and L. Mahadevan, as they provide a perspective on the influence of DArcy Thompsons work and an overview of the articles in this issue.. ...
1895 The extent of incorporation of exogenously administered bromodeoxyuridine (BrdU) into DNA is an indicator of the rate of DNA synthesis and cell proliferation. We developed and validated an HPLC method for quantification of BrdU in DNA, based on the separation of nucleosides and their detection by an online UV detector. The baseline separation of BrdU from both normal deoxyribonucleosides and ribonucleosides was achieved in a 60-min run on a reversed-phased C18 column, using a methanol/ammonium formate buffer, as the eluent. The limit of detection was about 0.01 nmole BrdU using a standard BrdU solution. DNA was enzymatically hydrolyzed to deoxyribonucleosides prior to HPLC. The method allowed the detection of background levels of BrdU incorporation into DNA (45 μg) of urinary bladder and duodenum of control rats after a continuous administration of a BrdU solution (50 mg/ml) at a flow rate of 10 μl/h for 4 d, using a subcutaneously implanted osmatic minipump. Compared with the levels ...
Neethi explained the current understanding of neurogenesis in the Sub Granular Zone of DG and the Sub Ventricular Zone of lateral ventricles as well as the impact of neurotherapeutics on neurogenesis. Currently there is no consensus if neurogenesis plays a causal role or is an after-effect in the treatment of neuropsychiatric disorders through antidepressants or ECS ...
Bachvaroff, R J. and Rapaport, F T., "Studies of mechanisms of antibody formation. Iii. Effects of 5-bromodeoxyuridine upon normal and neoplastic b-lymphocyte function." (1977). Subject Strain Bibliography 1977. 3428 ...
4-BROMO-2,3-DIFLUOROBENZENEBORONIC ACID 374790-99-5 NMR spectrum, 4-BROMO-2,3-DIFLUOROBENZENEBORONIC ACID H-NMR spectral analysis, 4-BROMO-2,3-DIFLUOROBENZENEBORONIC ACID C-NMR spectral analysis ect.
Cell Kinetics Signs Distribution Agreement with Rikaken Co., Ltd. Japan - read this article along with other careers information, tips and advice on BioSpace
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BACKGROUND: Receptors belonging to the epidermal growth factor receptor (EGFR) family transfer extracellular signals by homotypic and heterotypic receptor interaction and cross-activation. Cell differentiation, death, and proliferation are regulated via these receptor-tyrosine-kinases. However, the initial mechanisms that lead to signal specificity and diversity, which cause a defined cellular response, are incompletely understood. We investigated the recruitment of receptor complexes in two c-erbB2-overexpressing breast carcinoma cell lines, SK-BR-3 and BT474, after ligand binding and its effects on intracellular signal transduction and cell cycle regulation. METHODS: In order to analyze the coaggregation of receptors on the cell surface induced by specific growth factor treatment, we used the flow cytometric Foerster-type fluorescence resonance energy transfer (FRET) technique. Cell cycle kinetics were monitored flow cytometrically via the anti-BrdU technique and acitivation of intracellular ...
Murine neural stem cell (mNSC) proliferation. A) mNSCs were treated with different concentration (0nM, 1nM, 10nM, 100nM) of methylphenidate (MPH). The percentage (% of control) of proliferated cells was determined 28 h after induction of neuronal proliferation and MPH treatment. The amount of proliferating cells was analyzed by counting the Bromodeoxyurdine (BrdU) positive cells in comparison to the total number of cells using the Mann-Whitney (U-Rang) Test; --- = p ,0.05; n = 20; five independent experiments and four wells/slide of each concentration were evaluated. B) An example of an immunocytochemistry staining of BrdU in a control sample (no MPH treatment). The white arrow points to a BrdU positive cell (red) and the white arrow points to a BrdU negative cell (blue); 10x magnification. ...
潘永良.,M.Li.,X.Yi.,Q.Zhao.,C.Lieberwirth.,...&张知彬.(2013).Scatter Hoarding and Hippocampal Cell Proliferation in Siberian Chipmunks.Neuroscience,255,76-85 ...
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
Purpose : To optimize the growth condition of porcine corneal endothelial cells (PCEC), we evaluated the effect of coculturing with a feeder layer (irradiated 3T3 fibroblasts) with the addition of various exogenous factors, such as epidermal growth factor (EGF), nerve growth factor (NGF), bovine pituitary extract (BPE), ascorbic acid, and chondroitin sulfate, on cell proliferation, size, and morphology. Methods : PCEC cultures were seeded at an initial cell density of 400 cells/cm2 in the presence or absence of 20,000 murine-irradiated 3T3 fibroblast/cm2 in the classic media Dulbeccos Modified Eagles Medium (DMEM) supplemented with 20% fetal bovine serum (FBS). Mean cell size and bromodeoxyuridine incorporation was assessed at various passages. Growth-promoting factors were studies by seeding PCEC at 8,000 cells/cm2 in DMEM with 20% FBS or Opti-MEM I supplemented with 4% FBS and one of the following additives: EGF (0.5, 5, 25 ng/ml), NGF (5, 20, 50 ng/ml), BPE (25, 50, 100, 200 μg/ml), ...
Around 15 years ago, researchers discovered that the adult rodent brain contains discrete populations of stem cells which continue to divide and produce new neurons throughout life. This discovery was an important one, as it overturned a persistent dogma in neuroscience which held that the adult mammalian brain cannot regenerate. Since then, neural stem cells…
Klebsiella terrigena BudR protein: a transcriptional activator controlling the butanediol fermentation pathway in Klebsiella terrigena; MW 32 kDa; amino acid sequence given in first source; GenBank Z48600
Mice. To obtain normal tissue of the gastric transition zones, C57BL/6 control mice were given a single i.p. injection of 150 mg/mL bromodeoxyuridine (BrdUrd) to label cells in S phase 1 h before being killed by cervical dislocation. To allow optimal orientation of the gastric tissue, flat stomachs were prepared according to the method described by Lee et al. ( 12).. To evaluate the role of Bmpr1a signaling in the gastric mucosa, we used a conditional knockout approach using the Cre/loxP system. We used a mouse with a conditional null allele of Bmpr1a (Bmpr1afx/fx) that was previously generated and characterized ( 10, 13, 14). In these mice, LoxP sites have been introduced in the first and second introns of the Bmpr1a locus. It was previously shown that when these mice are crossed with CMV-Cre transgenic mice, the phenotype is identical to the Bmpr1a(−/−) mouse, indicating that deletion of exon 2 is sufficient to completely inactivate Bmpr1a function. For our experiment, the Bmpr1afx/fx ...
Universite de Sherbrooke Radiosensibilisation de lADN par le 5-bromodeoxyuridine : limportance de la structure et de la sequence de lADN Par Marie-Eve Dextraze Departement de medecine nucleaire et radiobiologie These presentee a la Faculte de medecine et des sciences de la sante en vue de lobtention du grade de philosophiae doctor (Ph.D.) en science des radiations et imagerie nucleaire Sherbrooke, Quebec, Canada 17juin2010 Jean-Paul Jay-Gerin, PhD Darel Hunting, PhD Richard Wagner, PhD Antonio Conconi, PhD Dindial Ramotar, PhD © Marie-Eve Dextraze, 2010 Membres du jury devaluation President du jury, Departement de medecine nucleaire et radiobiologie, FMSS Directeur de recherche, Departement de medecine nucleaire et radiobiologie, FMSS Directeur de recherche, Departement de medecine nucleaire et radiobiologie, FMSS Departement de microbiologic et infectiologie, FMSS Departement de Medecine et Specialites Medicales, Faculte de medecine, Universite de Montreal 1*1 Library and Archives Canada ...
An examination of 54 human brains suggests that adults dont grow new neurons in the hippocampus, contrary to several widely accepted studies.
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InCHi String: canonical SMILES: C1C(C(OC1N2C=CC(=O)NC2=O)CO)O. isomeric SMILES: C1[[email protected]@H]([[email protected]](O[[email protected]]1N2C=CC(=O)NC2=O)CO)O. PUBCHEM iupac NAME: PUBCHEM iupac SYSTEMATIC NAME ...
Company Name: Neurogenesis Inc, Stock Symbol: NEUN, Industry: Medical - Drugs, Total Posts: 797, Last Post: 10/9/2017 12:00:26 PM
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TY - JOUR. T1 - Fibroblast Growth Factor 14 Modulates the Neurogenesis of Granule Neurons in the Adult Dentate Gyrus. AU - Alshammari, Musaad A.. AU - Alshammari, Tahani K.. AU - Nenov, Miroslav N.. AU - Scala, Federico. AU - Laezza, Fernanda. PY - 2016/12/1. Y1 - 2016/12/1. N2 - Adult neurogenesis, the production of mature neurons from progenitor cells in the adult mammalian brain, is linked to the etiology of neurodegenerative and psychiatric disorders. However, a thorough understanding of the molecular elements at the base of adult neurogenesis remains elusive. Here, we provide evidence for a previously undescribed function of fibroblast growth factor 14 (FGF14), a brain disease-associated factor that controls neuronal excitability and synaptic plasticity, in regulating adult neurogenesis in the dentate gyrus (DG). We found that FGF14 is dynamically expressed in restricted subtypes of sex determining region Y-box 2 (Sox2)-positive and doublecortin (DCX)-positive neural progenitors in the DG. ...
We showed previously that DNA replication initiates at multiple sites in the 5-kb histone gene repeating unit in early embryos of Drosophila melanogaster. The present report shows evidence that replication in the same chromosomal region initiates at multiple sites in tissue culture cells as well. First, we analyzed replication intermediates by the two-dimensional gel electrophoretic replicon mapping method and detected bubble-form replication intermediates for all fragments restricted at different sites in the repeating unit. Second, we analyzed bromodeoxyuridine-labeled nascent strands amplified by the polymerase chain reaction method and detected little differences in the size distribution of nascent strands specific to six short segments located at different sites in the repeating unit. These results strongly suggest that DNA replication initiates at multiple sites located within the repeating unit. We also found several replication pause sites located at 5 upstream regions of some histone ...
TY - JOUR. T1 - Monitoring tumor cell proliferation by targeting DNA synthetic processes with thymidine and thymidine analogs. AU - Schwartz, Jeffrey L.. AU - Tamura, Yasuko. AU - Jordan, Robert. AU - Grierson, John R.. AU - Krohn, Kenneth. PY - 2003/12. Y1 - 2003/12. N2 - The use of radiolabeled thymidine (TdR) and thymidine analogs as PET-based tracers of tumor growth rate is based on the assumption that measurement of uptake of these nucleosides, a function primarily of thymidine kinase-1 (TK 1) activity, provides an accurate measure of active cell proliferation in tumors. The goal of this study was to test this hypothesis and determine how well these tracers track changes in proliferation of tumor cells. Methods: TK1 activity; S-phase fraction; and uptake of TdR, 3′-deoxy-3′-fluorothymidine (FLT), and 2′-fluoro-5-methyl-1- (β-D-2-arabino-furanosyl) uracil (FMAU) were determined in plateau-phase and exponentially growing cultures of 3 human and 3 murine tumor cell lines. Results: TK1 ...

*Harold M. Weintraub

The effects of bromodeoxyuridine on cell differentiation (conversion of a primitive cell into a more specialized cell) were ... Weintraub, H; Campbell, GL; Holtzer, H (1972). "Identification of a developmental program using bromodeoxyuridine". J Mol Biol ...

*Macrostomum lignano

A bromodeoxyuridine analysis". 226: 231-241. Rieger, R; Ladurner, P (2003). "The significance of muscle cells for the origin of ...

*Mutation

Base analogs (e.g., Bromodeoxyuridine (BrdU)). *Alkylating agents (e.g., N-ethyl-N-nitrosourea (ENU)). These agents can mutate ...

*Mutation

Induced mutations on the molecular level can be caused by: Chemicals Hydroxylamine Base analogs (e.g., Bromodeoxyuridine (BrdU ...

*Adult neurogenesis

Kuhn HG, Cooper-Kuhn CM (2007). "Bromodeoxyuridine and the detection of neurogenesis". Curr Pharmaceutical Biotechnol. 8: 127- ...

*Single-cell DNA template strand sequencing

During S-phase cells are treated with bromodeoxyuridine (BrdU) which is then incorporated into their nascent DNA, acting as a ... LAMBERT, BO; HANSSON, KERSTIN; LINDSTEN, JAN; STEN, MARGARETA; WERELIUS, BARBRO (12 February 2009). "Bromodeoxyuridine-induced ...

*5-Bromouridine

5-Bromouracil Bromodeoxyuridine 5-bromouridine in Linstrom, Peter J.; Mallard, William G. (eds.); NIST Chemistry WebBook, NIST ...

*Marianne Frommer

... based on incorporation of bromodeoxyuridine into single-stranded probes. The method was used to localise satellite repeats very ... Localisation of satellite DNA sequences on human metaphase chromosomes using bromodeoxyuridine-labelled probes. Chromosoma 97: ...

*5-Ethynyl-2'-deoxyuridine

Unlike the commonly used bromodeoxyuridine, EdU detection requires no heat or acid treatment. Chehrehasa, F; Meedeniya, AC; ...

*Oxalotrophic

"Identification of active oxalotrophic bacteria by Bromodeoxyuridine DNA labeling in a microcosm soil experiments". FEMS ...

*Georg F. Striedter

... pulse-labeling chick embryos with bromodeoxyuridine". Journal of Neuroscience. 20 (21): 8021-8030. PMID 11050123. Striedter, G. ...

*Androgen

Neural injections of Bromodeoxyuridine (BrdU) were applied to males of both groups to test for neurogenesis. Analysis showed ...

*Thymidine kinase in clinical chemistry

There it is detected with ELISA technique: The wells are filled with a solution of a monoclonal antibody to bromo-deoxyuridine ... The DiviTum assay from Biovica International uses another thymidine analogue, bromodeoxyuridine, as substrate to the enzyme. ...

*Thymidine kinase

Merrick CJ (2015). "Transfection with thymidine kinase permits bromodeoxyuridine labelling of DNA replication in the human ...

*Thymidine

Bromodeoxyuridine (BrdU) is another thymidine analog that is often used for the detection of proliferating cells in living ...

*Hoechst stain

Hoechst 33342 and 33258 are quenched by bromodeoxyuridine (BrdU), which is commonly used to detect dividing cells. Hoechst ...

*Sister chromatid exchange

The Giemsa staining was able to stain due to the presence of bromodeoxyuridine analogous base which was introduced to the ...

*Chromosome combing

... bromodeoxyuridine) into the nascent DNA, then fluorescently detecting it. As replication forks spread bidirectionally from ...

*HAT medium

Thus, a cell lacking TK is resistant to bromodeoxyuridine (BrdU) and a cell lacking HGPRT is resistant to 6-thioguanine (6-TG) ...

*Immortal DNA strand hypothesis

... or parental DNA strands with a DNA label such as tritiated thymidine or bromodeoxyuridine (BrdU). These types of DNA labels ...

*Chromosomal fragile site

... which are induced by bromodeoxyuridine (BrdU) or distamycin A, an antibiotic that preferentially binds to AT-pairs of DNA. The ...

*Stem cell marker

... of ROSA26 mice beta1-integrin bromodeoxyuridine c-kit (CD117) c-Met C1qR(p) END (CD105) PROM1 (CD133) ALCAM (CD166) ITGB1 (CD29 ...

*Renal stem cell

Using stain-retaining assay (with bromodeoxyuridine, or BrdU), a low-cycling cell population was found in the papillary region ...

*List of MeSH codes (D13)

... bromodeoxyuridine MeSH D13.570.230.430.432 --- floxuridine MeSH D13.570.230.430.609 --- idoxuridine MeSH D13.570.230.500 --- ... bromodeoxyuridine MeSH D13.570.685.852.300.350 --- floxuridine MeSH D13.570.685.852.300.400 --- idoxuridine MeSH D13.570. ...

*Bromodeoxyuridine

... (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd, broxuridine) is a synthetic nucleoside that is an analog of ... Bromodeoxyuridine releases gene silencing[clarification needed] caused by DNA methylation. BrdU can also be used to identify ... "Correlation Between Bromodeoxyuridine- Labeling Indices and Patient Prognosis in Cerebral Astrocytic Tumors of Adults". Cancer ... "Cell Kinetic Studies of In Situ Human Brain Tumors With Bromodeoxyuridine". Cytometry. 6 (6): 627-632. doi:10.1002/cyto. ...

Pairing properties of bromouracil and repair of bromouracil-containing DNA. Possible utilization of bromodeoxyuridine...Pairing properties of bromouracil and repair of bromouracil-containing DNA. Possible utilization of bromodeoxyuridine...

Possible utilization of bromodeoxyuridine triphosphate for site-directed mutagenesis. M Muller, J Martial, W G Verly ... Possible utilization of bromodeoxyuridine triphosphate for site-directed mutagenesis Message Subject (Your Name) has forwarded ...
more infohttp://www.biochemj.org/content/253/3/637

Bromodeoxyuridine - WikipediaBromodeoxyuridine - Wikipedia

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU, BUdR, BrdUrd, broxuridine) is a synthetic nucleoside that is an analog of ... Bromodeoxyuridine releases gene silencing[clarification needed] caused by DNA methylation. BrdU can also be used to identify ... "Correlation Between Bromodeoxyuridine- Labeling Indices and Patient Prognosis in Cerebral Astrocytic Tumors of Adults". Cancer ... "Cell Kinetic Studies of In Situ Human Brain Tumors With Bromodeoxyuridine". Cytometry. 6 (6): 627-632. doi:10.1002/cyto. ...
more infohttps://en.wikipedia.org/wiki/Bromodeoxyuridine

The effects of bromodeoxyuridine and bromouracil on regeneration in Hydra | SpringerLinkThe effects of bromodeoxyuridine and bromouracil on regeneration in Hydra | SpringerLink

... bromouracil and bromodeoxyuridine, have been studied. Bromouracil inhibits tentacl ... The effects of bromodeoxyuridine and bromouracil on regeneration inHydra *M. H. Goyns1. & ... Goyns, M.H., Stanisstreet, M. The effects of bromodeoxyuridine and bromouracil on regeneration inHydra . W. Roux Archiv f. ... Lasher, R., Cahn, R. D.: Effects of 5-bromodeoxyuridine on the differentiation of chondrocytes in vitro. Develop. Biol.19, 415- ...
more infohttps://link.springer.com/article/10.1007%2FBF00574294

anti-Bromodeoxyuridine/BrdU, Biotin, Clone: SPM537, Novus Biologicals 100
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anti-Bromodeoxyuridine/BrdU, Biotin, Clone: SPM537, Novus Biologicals 100 Tests; Biotin Life Sciences:Antibodies:Primary ... Bromodeoxyuridine/BrdU Monoclonal antibody specifically detects Bromodeoxyuridine/BrdU in All Species samples. It is validated ... It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU ...
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Bromodeoxyuridine/BrdU Antibody (NB500-235): Novus BiologicalsBromodeoxyuridine/BrdU Antibody (NB500-235): Novus Biologicals

Sheep Polyclonal Anti-Bromodeoxyuridine/BrdU Antibody Proliferation Marker cited in 15 publications. Validated: ICC/IF, IHC, ... Bromodeoxyuridine/BrdU Antibody Summary. Immunogen. Bromodeoxyuridine (BrdU) coupled to keyhole limpet hemocyanin (KLH). ... BrDU (Bromodeoxyuridine). The thymidine synthetic nucleoside analogue bromodeoxyuridine (BrdU) has a long, colorful history of ... Blogs on Bromodeoxyuridine/BrdU. Check out the latest blog posts on Bromodeoxyuridine/BrdU. ...
more infohttps://www.novusbio.com/products/bromodeoxyuridine-brdu-antibody_nb500-235?utm_source=biocompare&utm_medium=referral&utm_campaign=product&utm_term=primaryantibodies

Bromodeoxyuridine (BrdU) - OpenWetWareBromodeoxyuridine (BrdU) - OpenWetWare

Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside analogue of thymidine. BrdU is commonly used to ... Retrieved from "https://openwetware.org/mediawiki/index.php?title=Bromodeoxyuridine_(BrdU)&oldid=379477" ...
more infohttps://openwetware.org/wiki/Bromodeoxyuridine_

Bromodeoxyuridine/BrdU Antibody (BRD494) [Biotin] (NBP2-34566B): Novus BiologicalsBromodeoxyuridine/BrdU Antibody (BRD494) [Biotin] (NBP2-34566B): Novus Biologicals

Mouse Monoclonal Anti-Bromodeoxyuridine/BrdU Antibody (BRD494) [Biotin]. Proliferation Marker. Validated: ICC/IF, IHC-Fr, IHC-P ... Check out the latest blog posts on Bromodeoxyuridine/BrdU.. BrDU (Bromodeoxyuridine). The thymidine synthetic nucleoside ... Bromodeoxyuridine/BrdU Antibody (BRD494) [Biotin] Summary. Immunogen. Bromodeoxyuridine (BrdU) conjugated to KLH ... Reviews for Bromodeoxyuridine/BrdU Antibody (NBP2-34566B) (0) There are no reviews for Bromodeoxyuridine/BrdU Antibody (NBP2- ...
more infohttps://www.novusbio.com/products/bromodeoxyuridine-brdu-antibody-brd494_nbp2-34566b

High-speed photodamage cell selection using bromodeoxyuridine/Hoechst 33342 photosensitized cell killing - Herweijer - 1988 -...High-speed photodamage cell selection using bromodeoxyuridine/Hoechst 33342 photosensitized cell killing - Herweijer - 1988 -...

High-speed photodamage cell selection using bromodeoxyuridine/Hoechst 33342 photosensitized cell killing†‡. Authors. *. Hans ...
more infohttp://onlinelibrary.wiley.com/doi/10.1002/cyto.990090208/abstract

HPLC/UV or HPLC/32P-postlabeling methods for quantification of bromodeoxyuridine in DNA | Cancer ResearchHPLC/UV or HPLC/32P-postlabeling methods for quantification of bromodeoxyuridine in DNA | Cancer Research

HPLC/UV or HPLC/32P-postlabeling methods for quantification of bromodeoxyuridine in DNA. M. Vijayaraj Reddy, John E. Barnum, ... The extent of incorporation of exogenously administered bromodeoxyuridine (BrdU) into DNA is an indicator of the rate of DNA ... HPLC/UV or HPLC/32P-postlabeling methods for quantification of bromodeoxyuridine in DNA ... HPLC/UV or HPLC/32P-postlabeling methods for quantification of bromodeoxyuridine in DNA ...
more infohttp://cancerres.aacrjournals.org/content/64/7_Supplement/435.4

Use of Bromodeoxyuridine to Study White Blood Cell Replication and Survival in HIV-Infected Patients - No Study Results Posted ...Use of Bromodeoxyuridine to Study White Blood Cell Replication and Survival in HIV-Infected Patients - No Study Results Posted ...

Use of Bromodeoxyuridine to Study White Blood Cell Replication and Survival in HIV-Infected Patients. The safety and scientific ...
more infohttps://clinicaltrials.gov/ct2/show/results/NCT00001650

Mouse Monoclonal anti-BrdU (Bromodeoxyuridine) (BRD469) | BiotiumMouse Monoclonal anti-BrdU (Bromodeoxyuridine) (BRD469) | Biotium

Mouse Monoclonal anti-BrdU (Bromodeoxyuridine) (BRD469) This antibody reacts with Bromodeoxyuridine (BrdU) in single stranded ... This antibody reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded ...
more infohttps://biotium.com/product/monoclonal-anti-brdu-bromodeoxyuridine-brd469/

Cell Cycle Analysis | BrdU Incorporation in DNA | Monoclonal Anti-Bromodeoxyuridine (Clone BU6-4)Cell Cycle Analysis | BrdU Incorporation in DNA | Monoclonal Anti-Bromodeoxyuridine (Clone BU6-4)

Monoclonal Anti-Bromodeoxyuridine (Clone BU6-4). Monoclonal Anti-Bromodeoxyuridine (Clone BU6-4) is often used in cell cycle ... The monoclonal anti-bromodeoxyuridine antibody can be used in Western blot analysis under non-reducing and non-heating ... Immunogen: Bromodeoxyuridine-BSA conjugate *Immunologic host: BALB/c mouse *antibody subclass: IgG1 *Antibody titer: 1:2,000 ... analysis studies; the level of bromodeoxyuridine (BrdU) incorporation into DNA is a direct parameter of cell number and growth ...
more infohttp://www.clontech.com/US/Products/Cell_Biology_and_Epigenetics/Miscellaneous/Primary_Antibodies_A-K/Bromodeoxyuridine?sitex=10020:22372:US&PEBCL1=6v1uYiV6cGacQI9LdECiMoa203&PEBCL1_pses=ZG321263123A556FE6934294AFC7280C8487D94C36957E74E15F0F5354A8837037E257ABD54B618E65761B31BAB35F69ECB43FD8B2BFE2E755

Effects of bromodeoxyuridine, cytosine arabinoside and Colcemid upon in vitro development of mouse blastocysts | DevelopmentEffects of bromodeoxyuridine, cytosine arabinoside and Colcemid upon in vitro development of mouse blastocysts | Development

Effects of bromodeoxyuridine, cytosine arabinoside and Colcemid upon in vitro development of mouse blastocysts ... Effects of bromodeoxyuridine, cytosine arabinoside and Colcemid upon in vitro development of mouse blastocysts ... Effects of bromodeoxyuridine, cytosine arabinoside and Colcemid upon in vitro development of mouse blastocysts ... Effects of bromodeoxyuridine, cytosine arabinoside and Colcemid upon in vitro development of mouse blastocysts ...
more infohttp://dev.biologists.org/content/34/2/467

Biophysical and computational approaches to unravel the molecular interaction mechanism of bromodeoxyuridine, a proliferative...Biophysical and computational approaches to unravel the molecular interaction mechanism of bromodeoxyuridine, a proliferative...

Abstract The interaction of a nucleoside analogue bromodeoxyuridine (BrdU) with human serum albumin (HSA) was studied to ... Bromodeoxyuridine Human serum albumin Drug-protein interaction Fluorescence quenching Molecular modelling Electronic ... The interaction of a nucleoside analogue bromodeoxyuridine (BrdU) with human serum albumin (HSA) was studied to investigate the ... Biophysical and computational approaches to unravel the molecular interaction mechanism of bromodeoxyuridine, a proliferative ...
more infohttps://link.springer.com/article/10.1007%2Fs00706-019-02518-z

Isolation by restriction endonuclease digestion and base-specific affinity chromatography of rat-embryo DNA sequences...Isolation by restriction endonuclease digestion and base-specific affinity chromatography of rat-embryo DNA sequences...

Effect of 5-bromodeoxyuridine on the appearance of the liver isoenzyme of pyruvate kinase Biochem J (June, 1984) ... Control and bromodeoxyuridine-containing rat-embryo-cell DNA were digested by the restriction endonucleases Hpa II and Msp I ... base-specific affinity chromatography of rat-embryo DNA sequences disproportionately enriched in virogenic bromodeoxyuridine ... base-specific affinity chromatography of rat-embryo DNA sequences disproportionately enriched in virogenic bromodeoxyuridine. ...
more infohttps://portlandpress.com/bioscirep/article-abstract/1/2/107/53407/Isolation-by-restriction-endonuclease-digestion?redirectedFrom=fulltext

Clinical outcome and bromodeoxyuridine derived proliferation indices in 100 colonic and rectal carcinomas  - ePrints SotonClinical outcome and bromodeoxyuridine derived proliferation indices in 100 colonic and rectal carcinomas - ePrints Soton

Cutress, R.I., Mullee, M.A. and Rew, D.A. (2002) Clinical outcome and bromodeoxyuridine derived proliferation indices in 100 ... Clinical outcome and bromodeoxyuridine derived proliferation indices in 100 colonic and rectal carcinomas ... Aim: In vivo labelling of human colonic and rectal tumours with bromodeoxyuridine (BrdUrd) and analysis by flow cytometry (FCM ... Aim: In vivo labelling of human colonic and rectal tumours with bromodeoxyuridine (BrdUrd) and analysis by flow cytometry (FCM ...
more infohttps://eprints.soton.ac.uk/24316/

IDEALS @ Illinois: The development of a monoclonal antibody against 5-Bromodeoxyuridine and its applications in research...IDEALS @ Illinois: The development of a monoclonal antibody against 5-Bromodeoxyuridine and its applications in research...

The development of a monoclonal antibody against 5-Bromodeoxyuridine and its applications in research microfilm. Welcome to the ... The development of a monoclonal antibody against 5-Bromodeoxyuridine and its applications in research microfilm. Schmeda, Peter ... The development of a monoclonal antibody against 5-Bromodeoxyuridine and its applications in research microfilm. ...
more infohttps://www.ideals.illinois.edu/handle/2142/93670

Bioavailability of bromodeoxyuridine in dogs and toxicity in rats. - Semantic ScholarBioavailability of bromodeoxyuridine in dogs and toxicity in rats. - Semantic Scholar

Bioavailability of bromodeoxyuridine in dogs and toxicity in rats. by Surasak Phuphanich et al. ... A New Method for In Vitro Detection of Bromodeoxyuridine in Serum: A Proof of Concept in a Songbird Species, the Canary. * ... Bioavailability of bromodeoxyuridine in dogs and toxicity in rats.. @article{Phuphanich1985BioavailabilityOB, title={ ... Bioavailability of bromodeoxyuridine in dogs and toxicity in rats.}, author={Surasak Phuphanich and V. A. Levin}, journal={ ...
more infohttps://www.semanticscholar.org/paper/Bioavailability-of-bromodeoxyuridine-in-dogs-and-in-Phuphanich-Levin/edfb7695bfb3d36a38ebb212ed817917564a3857

Bromodeoxyuridine (BrdU) (Proliferation Marker) Antibody - With BSA and Azide -  Mouse Monoclonal Antibody [Clone BRD469 +...Bromodeoxyuridine (BrdU) (Proliferation Marker) Antibody - With BSA and Azide - Mouse Monoclonal Antibody [Clone BRD469 +...

Bromodeoxyuridine (BrdU) (Proliferation Marker) Antibody - With BSA and Azide, Mouse Monoclonal Antibody [Clone BRD469 + BRD494 ... Bromodeoxyuridine (BrdU) (Proliferation Marker) Antibody - With BSA and Azide is for research use only and not for use in ... It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU ... Bromodeoxyuridine (BrdU) (Proliferation Marker) Antibody - With BSA and Azide. Mouse Monoclonal Antibody [Clone BRD469 + BRD494 ...
more infohttp://www.abgent.com/products/AH10977-Bromodeoxyuridine-BrdU-Proliferation-Marker-Antibody-With-BSA-and-Azide

Microbiology Society Journals | Inhibition of Sporulation in Bacillus subtilis by Bromodeoxyuridine and the Effect on DNA...Microbiology Society Journals | Inhibition of Sporulation in Bacillus subtilis by Bromodeoxyuridine and the Effect on DNA...

f Inhibition of Sporulation in Bacillus subtilis by Bromodeoxyuridine and the Effect on DNA Replication ... Inhibition of Sporulation in Bacillus subtilis by Bromodeoxyuridine and the Effect on DNA Replication, Page 1 of 1 ...
more infohttp://mic.microbiologyresearch.org/content/journal/micro/10.1099/00221287-132-2-493

Cytochemistry for bromodeoxyuridine/DNA analysis: stoichiometry and sensitivity. - Semantic ScholarCytochemistry for bromodeoxyuridine/DNA analysis: stoichiometry and sensitivity. - Semantic Scholar

... describes an improved immunochemical procedure for staining cells in suspension for amount of incorporated bromodeoxyuridine ( ... Improved bromodeoxyuridine/DNA analysis by anti-BudR monoclonal antibody versus right angle light scatter. Marco Vitale, Luca M ... Cytochemistry for bromodeoxyuridine/DNA analysis: stoichiometry and sensitivity.. @article{Dolbeare1985CytochemistryFB, title={ ... Cytochemistry for bromodeoxyuridine/DNA analysis: stoichiometry and sensitivity.}, author={Frank Dolbeare and Wolfgang Beisker ...
more infohttps://www.semanticscholar.org/paper/Cytochemistry-for-bromodeoxyuridine%2FDNA-analysis%3A-Dolbeare-Beisker/5297918e9b0fff68062c99c1f76f4ef22a4a6052

Mouse anti BrdU (bromodeoxyuridine) - Exalpha Biologicals inc.Mouse anti BrdU (bromodeoxyuridine) - Exalpha Biologicals inc.

Mouse anti BrdU (bromodeoxyuridine). Catalogue number: X1028. Synonyms. Anti-Bromodeoxyuridine Mouse MoBU Monoclonal Antibody ... Bromodeoxyuridine conjugated to Helix Pomatia Haemocyanin used to immunize BALB/c mice Purification Method. Protein A/G ... Application of bromodeoxyuridine (BrdU) and anti-BrdU monoclonal antibody for the in vivo analysis of proliferative ... Oncology 1991, 48, 285-289 2. Wilson, G., Cell kinetic studies using a monoclonal antibody to bromodeoxyuridine. Methods Mol. ...
more infohttps://www.exalpha.com/product/brdu-bromodeoxyuridine-2/

Abnormal DNA synthesis in polyamine deficient cells revealed by bromodeoxyuridine-flow cytometry technique - Lund UniversityAbnormal DNA synthesis in polyamine deficient cells revealed by bromodeoxyuridine-flow cytometry technique - Lund University

Abnormal DNA synthesis in polyamine deficient cells revealed by bromodeoxyuridine-flow cytometry technique. Research output: ... the cells were labelled for 15-120 min with the thymidine analogue bromodeoxyuridine (BrdUrd) and they were then fixed directly ...
more infohttps://portal.research.lu.se/portal/en/publications/abnormal-dna-synthesis-in-polyamine-deficient-cells-revealed-by-bromodeoxyuridineflow-cytometry-technique
  • Bromodeoxyuridine (BrdU) coupled to keyhole limpet hemocyanin (KLH). (novusbio.com)
  • The distribution of DNA synthesising cells in the crypts of the epithelium in human small and large bowel after injection of bromodeoxyuridine into patients has been studied in relation to the position of the cells in the crypt using immunohistochemistry. (openrepository.com)
  • Coleman, J. R., Coleman, W. A., Hartline, E. J. H.: A clonal study of the reversible inhibition of muscle differentiation by the halogenated thymidine analogue 5-bromodeoxyuridine. (springer.com)
  • In order to relate changes in the expression of these genes to changes in cell kinetics within the lesions, we used bromodeoxyuridine to label cells during the S phase as a measure of the proliferative index and counted apoptotic cells based on morphology to estimate cell loss. (biomedcentral.com)
  • Lasher, R., Cahn, R. D.: Effects of 5-bromodeoxyuridine on the differentiation of chondrocytes in vitro. (springer.com)
  • Mens således anvendelse av in vitro cellekultur modeller har gitt mekanistiske innsikt, er en vanlig kritikk at de ikke rekapitulere kompleksiteten forbundet med en mikrovaskulær nettverk. (jove.com)
  • Replication not inhibited by 5-bromodeoxyuridine. (cdc.gov)
  • Proliferation in human gastrointestinal epithelium using bromodeoxyuridine in vivo: data for different sites, proximity to a tumour, and polyposis coli. (openrepository.com)
  • Measurement of in vivo proliferation in human colorectal mucosa using bromodeoxyuridine. (openrepository.com)
  • Bromodeoxyuridine releases gene silencing[clarification needed] caused by DNA methylation. (wikipedia.org)
  • An elevated bromodeoxyuridine labeling index is suggestive of a rapid growth rate of meningioma and a greater incidence of recurrence following surgical resection. (wikidoc.org)
  • Mouse blastocysts in culture have been treated with increasing concentrations of cytosine arabinoside, bromodeoxyuridine or Colcemid. (biologists.org)