Bromodeoxyuridine
Cell Division
Antimetabolites
Bromouracil
Cell Count
DNA
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Cell Cycle
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
Neurogenesis
Immunohistochemistry
S Phase
Proliferating Cell Nuclear Antigen
Nuclear antigen with a role in DNA synthesis, DNA repair, and cell cycle progression. PCNA is required for the coordinated synthesis of both leading and lagging strands at the replication fork during DNA replication. PCNA expression correlates with the proliferation activity of several malignant and non-malignant cell types.
Cell Differentiation
Idoxuridine
Cells, Cultured
Stem Cells
Dentate Gyrus
GRAY MATTER situated above the GYRUS HIPPOCAMPI. It is composed of three layers. The molecular layer is continuous with the HIPPOCAMPUS in the hippocampal fissure. The granular layer consists of closely arranged spherical or oval neurons, called GRANULE CELLS, whose AXONS pass through the polymorphic layer ending on the DENDRITES of PYRAMIDAL CELLS in the hippocampus.
Sister Chromatid Exchange
An exchange of segments between the sister chromatids of a chromosome, either between the sister chromatids of a meiotic tetrad or between the sister chromatids of a duplicated somatic chromosome. Its frequency is increased by ultraviolet and ionizing radiation and other mutagenic agents and is particularly high in BLOOM SYNDROME.
Rats, Sprague-Dawley
Mitosis
Neurons
Cell Survival
Staining and Labeling
Flow Cytometry
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Apoptosis
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Nestin
Ultraviolet Rays
That portion of the electromagnetic spectrum immediately below the visible range and extending into the x-ray frequencies. The longer wavelengths (near-UV or biotic or vital rays) are necessary for the endogenous synthesis of vitamin D and are also called antirachitic rays; the shorter, ionizing wavelengths (far-UV or abiotic or extravital rays) are viricidal, bactericidal, mutagenic, and carcinogenic and are used as disinfectants.
Deoxyuridine
Epithelial Cells
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
Nucleic Acid Renaturation
Azaguanine
Thymidine Kinase
Thymine Nucleotides
Glial Fibrillary Acidic Protein
Radiation Effects
Ki-67 Antigen
Floxuridine
An antineoplastic antimetabolite that is metabolized to fluorouracil when administered by rapid injection; when administered by slow, continuous, intra-arterial infusion, it is converted to floxuridine monophosphate. It has been used to treat hepatic metastases of gastrointestinal adenocarcinomas and for palliation in malignant neoplasms of the liver and gastrointestinal tract.
Cell Movement
Hippocampus
A curved elevation of GRAY MATTER extending the entire length of the floor of the TEMPORAL HORN of the LATERAL VENTRICLE (see also TEMPORAL LOBE). The hippocampus proper, subiculum, and DENTATE GYRUS constitute the hippocampal formation. Sometimes authors include the ENTORHINAL CORTEX in the hippocampal formation.
Cell Nucleus
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Carcinogens
Disease Models, Animal
Fluorescent Antibody Technique
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Autoradiography
Hyperplasia
In Situ Nick-End Labeling
An in situ method for detecting areas of DNA which are nicked during APOPTOSIS. Terminal deoxynucleotidyl transferase is used to add labeled dUTP, in a template-independent manner, to the 3 prime OH ends of either single- or double-stranded DNA. The terminal deoxynucleotidyl transferase nick end labeling, or TUNEL, assay labels apoptosis on a single-cell level, making it more sensitive than agarose gel electrophoresis for analysis of DNA FRAGMENTATION.
Centrifugation, Density Gradient
Rats, Wistar
Culture Techniques
Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.
Intermediate Filament Proteins
Fibroblasts
Intestinal Mucosa
Papilloma
Dose-Response Relationship, Drug
Neuroglia
The non-neuronal cells of the nervous system. They not only provide physical support, but also respond to injury, regulate the ionic and chemical composition of the extracellular milieu, participate in the BLOOD-BRAIN BARRIER and BLOOD-RETINAL BARRIER, form the myelin insulation of nervous pathways, guide neuronal migration during development, and exchange metabolites with neurons. Neuroglia have high-affinity transmitter uptake systems, voltage-dependent and transmitter-gated ion channels, and can release transmitters, but their role in signaling (as in many other functions) is unclear.
Neural Stem Cells
Interphase
RNA, Messenger
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
4-Nitroquinoline-1-oxide
Cricetinae
G1 Phase
Neoplasms, Experimental
Deoxyribonucleotides
Cell Transformation, Neoplastic
Nucleic Acid Denaturation
Disruption of the secondary structure of nucleic acids by heat, extreme pH or chemical treatment. Double strand DNA is "melted" by dissociation of the non-covalent hydrogen bonds and hydrophobic interactions. Denatured DNA appears to be a single-stranded flexible structure. The effects of denaturation on RNA are similar though less pronounced and largely reversible.
Tyrosine Transaminase
An enzyme that catalyzes the conversion of L-TYROSINE and 2-oxoglutarate to 4-hydroxyphenylpyruvate and L-GLUTAMATE. It is a pyridoxal-phosphate protein. L-PHENYLALANINE is hydroxylated to L-tyrosine. The mitochondrial enzyme may be identical with ASPARTATE AMINOTRANSFERASES (EC 2.6.1.1.). Deficiency of this enzyme may cause type II Tyrosinemia (see TYROSINEMIAS). EC 2.6.1.5.
Chick Embryo
Mice, Transgenic
Mice, Knockout
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
Phenotype
Vimentin
Fibroblast Growth Factor 2
A single-chain polypeptide growth factor that plays a significant role in the process of WOUND HEALING and is a potent inducer of PHYSIOLOGIC ANGIOGENESIS. Several different forms of the human protein exist ranging from 18-24 kDa in size due to the use of alternative start sites within the fgf-2 gene. It has a 55 percent amino acid residue identity to FIBROBLAST GROWTH FACTOR 1 and has potent heparin-binding activity. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor (FIBROBLAST GROWTH FACTOR 1).
Phosphopyruvate Hydratase
Lateral Ventricles
Cavity in each of the CEREBRAL HEMISPHERES derived from the cavity of the embryonic NEURAL TUBE. They are separated from each other by the SEPTUM PELLUCIDUM, and each communicates with the THIRD VENTRICLE by the foramen of Monro, through which also the choroid plexuses (CHOROID PLEXUS) of the lateral ventricles become continuous with that of the third ventricle.
Ependyma
Colon
Clone Cells
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
Tumor Cells, Cultured
Cytarabine
A pyrimidine nucleoside analog that is used mainly in the treatment of leukemia, especially acute non-lymphoblastic leukemia. Cytarabine is an antimetabolite antineoplastic agent that inhibits the synthesis of DNA. Its actions are specific for the S phase of the cell cycle. It also has antiviral and immunosuppressant properties. (From Martindale, The Extra Pharmacopoeia, 30th ed, p472)
Mice, Inbred Strains
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
The incorporation of 5-iodo-2'-deoxyuridine into the DNA of HeLa cells and the induction of alkaline phosphatase activity. (1/3497)
Inhibition of DNA synthesis during the period of exposure of HeLa cells to 5-iodo-2'-deoxyuridine (IUdR) inhibited the induction of alkaline phosphatase activity. This finding, taken together with previous findings that IUdR did not induce alkaline phosphatase activity in the presence of 2-fold molar excess thymidinemonstrated that IUdR incorporation into DNA is correlated with the increase in alkaline phosphatase activity. With the exception of an interim period described in the text, induction of alkaline phosphatase activity was linearly related to medium concentrations of IUdR of up to at least 3 muM. However, the extent of IUdR substitution in DNA did not appear to be related to the degree of enzyme induction. Alkaline phosphatase activity continued to increase at medium concentrations of IUdR from 1 to 3 muM, while little further substitution of DNA occurred. (+info)High-throughput screening of small molecules in miniaturized mammalian cell-based assays involving post-translational modifications. (2/3497)
BACKGROUND: Fully adapting a forward genetic approach to mammalian systems requires efficient methods to alter systematically gene products without prior knowledge of gene sequences, while allowing for the subsequent characterization of these alterations. Ideally, these methods would also allow function to be altered in a temporally controlled manner. RESULTS: We report the development of a miniaturized cell-based assay format that enables a genetic-like approach to understanding cellular pathways in mammalian systems using small molecules, rather than mutations, as the source of gene-product alterations. This whole-cell immunodetection assay can sensitively detect changes in specific cellular macromolecules in high-density arrays of mammalian cells. Furthermore, it is compatible with screening large numbers of small molecules in nanoliter to microliter culture volumes. We refer to this assay format as a 'cytoblot', and demonstrate the use of cytoblotting to monitor biosynthetic processes such as DNA synthesis, and post-translational processes such as acetylation and phosphorylation. Finally, we demonstrate the applicability of these assays to natural-product screening through the identification of marine sponge extracts exhibiting genotype-specific inhibition of 5-bromodeoxyuridine incorporation and suppression of the anti-proliferative effect of rapamycin. CONCLUSIONS: We show that cytoblots can be used for high-throughput screening of small molecules in cell-based assays. Together with small-molecule libraries, the cytoblot assay can be used to perform chemical genetic screens analogous to those used in classical genetics and thus should be applicable to understanding a wide variety of cellular processes, especially those involving post-transitional modifications. (+info)Herpetic keratitis. Proctor Lecture. (3/3497)
Although much needs to be learned about the serious clinical problem of herpes infection of the cornea, we have come a long way. We now have effective topical antiviral drugs. We have animal models which, with a high degree of reliability, clearly predict the effect to be expected clinically in man, as well as the toxicity. We have systemically active drugs and the potential of getting highly active, potent, completely selective drugs, with the possibility that perhaps the source of viral reinfection can be eradicated. The biology of recurrent herpes and stromal disease is gradually being understood, and this understanding may result in new and better therapy of this devastating clinical disease. (+info)Immunochemical detection and isolation of DNA from metabolically active bacteria. (4/3497)
Most techniques used to assay the growth of microbes in natural communities provide no information on the relationship between microbial productivity and community structure. To identify actively growing bacteria, we adapted a technique from immunocytochemistry to detect and selectively isolate DNA from bacteria incorporating bromodeoxyuridine (BrdU), a thymidine analog. In addition, we developed an immunocytochemical protocol to visualize BrdU-labeled microbial cells. Cultured bacteria and natural populations of aquatic bacterioplankton were pulse-labeled with exogenously supplied BrdU. Incorporation of BrdU into microbial DNA was demonstrated in DNA dot blots probed with anti-BrdU monoclonal antibodies and either peroxidase- or Texas red-conjugated secondary antibodies. BrdU-containing DNA was physically separated from unlabeled DNA by using antibody-coated paramagnetic beads, and the identities of bacteria contributing to both purified, BrdU-containing fractions and unfractionated, starting-material DNAs were determined by length heterogeneity PCR (LH-PCR) analysis. BrdU-containing DNA purified from a mixture of DNAs from labeled and unlabeled cultures showed >90-fold enrichment for the labeled bacterial taxon. The LH-PCR profile for BrdU-containing DNA from a labeled, natural microbial community differed from the profile for the community as a whole, demonstrating that BrdU was incorporated by a taxonomic subset of the community. Immunocytochemical detection of cells with BrdU-labeled DNA was accomplished by in situ probing with anti-BrdU monoclonal antibodies and Texas red-labeled secondary antibodies. Using this suite of techniques, microbial cells incorporating BrdU into their newly synthesized DNA can be quantified and the identities of these actively growing cells can be compared to the composition of the microbial community as a whole. Since not all strains tested could incorporate BrdU, these methods may be most useful when used to gain an understanding of the activities of specific species in the context of their microbial community. (+info)Proteolipid protein gene product can be secreted and exhibit biological activity during early development. (5/3497)
A gene encoding myelin proteolipid protein (PLP) and its smaller isoform DM20 is expressed at least 1 week before myelination. Mutations within the gene cause abnormalities in the development of premyelinating oligodendrocytes, resulting in hypomyelinating disorders. These findings suggest a premyelinating function of the PLP gene products. We previously demonstrated that PLP gene expression is directly associated with secretion of a factor that increases the number of oligodendrocytes. Here we show that this activity is mediated by a secreted fragment containing the C-terminal portion of PLP. This factor increased the bromodeoxyuridine incorporation rate in both oligodendrocyte and astrocyte lineage cells; a synthetic peptide (PLP 215-232) exhibited a similar activity. Dose-response curves of PLP and PLP peptide showed maximum activities at a concentration in the picomolar range, which decreased at higher concentrations. These observations demonstrate that a secreted PLP gene product exerts biological activity at a premyelinating stage before the major induction of the gene. (+info)Embryonic and postnatal injections of bromodeoxyuridine produce age-dependent morphological and behavioral abnormalities. (6/3497)
The mitotic marker 5-bromodeoxyuridine (BrdU) was injected twice daily (60 mg/kg) into pregnant hooded rats on one of embryonic days (E) 11, 12, 13, 15, 17, or 21, or into rat pups on postnatal day (P) 10. The principal findings were the following: (1) BrdU exposure on E11 produces profound effects on body morphology, and animals must be fed a special diet because of chronic tooth abnormalities; (2) BrdU exposure at E17 or earlier produces a change in coat spotting pattern, the precise pattern varying with age; (3) BrdU exposure on E15 or earlier produces a reduction in both brain and body weight; (4) BrdU exposure on E17 or earlier reduces cortical thickness; (5) BrdU exposure on E11-E13 and at P10 reduces cerebellar size relative to cerebral size; (6) spatial learning is significantly affected after injections of BrdU at E11-E17, but the largest effect is on E17; (7) the deficit in spatial learning may be related in part to a reduction in visual acuity; and (8) skilled forelimb ability is most disrupted after BrdU exposure at E15 but is also impaired after injections on E13 or earlier. BrdU thus has teratological effects on body, brain, and behavior that vary with the developmental age of the fetus or infant. (+info)Capsaicin-sensitive C-fiber-mediated protective responses in ozone inhalation in rats. (7/3497)
To assess the role of lung sensory C fibers during and after inhalation of 1 part/million ozone for 8 h, we compared breathing pattern responses and epithelial injury-inflammation-repair in rats depleted of C fibers by systemic administration of capsaicin as neonates and in vehicle-treated control animals. Capsaicin-treated rats did not develop ozone-induced rapid, shallow breathing. Capsaicin-treated rats showed more severe necrosis in the nasal cavity and greater inflammation throughout the respiratory tract than did control rats exposed to ozone. Incorporation of 5-bromo-2'-deoxyuridine (a marker of DNA synthesis associated with proliferation) into terminal bronchiolar epithelial cells was not significantly affected by capsaicin treatment in rats exposed to ozone. However, when normalized to the degree of epithelial necrosis present in each rat studied, there was less 5-bromo-2'-deoxyuridine labeling in the terminal bronchioles of capsaicin-treated rats. These observations suggest that the ozone-induced release of neuropeptides does not measurably contribute to airway inflammation but may play a role in modulating basal and reparative airway epithelial cell proliferation. (+info)Retinal neurogenesis: the formation of the initial central patch of postmitotic cells. (8/3497)
We have investigated the relationship between the birthdate and the onset of differentiation of neurons in the embryonic zebrafish neural retina. Birthdates were established by a single injection of bromodeoxyuridine into embryos of closely spaced ages. Differentiation was revealed in the same embryos with a neuron-specific antibody, zn12. The first bromodeoxyuridine-negative (postmitotic) cells occupied the ganglion cell layer of ventronasal retina, where they formed a small cluster of 10 cells or less that included the first zn12-positive cells (neurons). New cells were recruited to both populations (bromodeoxyuridine-negative and zn12-positive) along the same front, similar to the unfolding of a fan, to produce a circular central patch of hundreds of cells in the ganglion cell layer about 9 h later. Thus the formation of this central patch, previously considered as the start of retinal neurogenesis, was actually a secondary event, with a developmental history of its own. The first neurons outside the ganglion cell layer also appeared in ventronasal retina, indicating that the ventronasal region was the site of initiation of all retinal neurogenesis. Within a column (a small cluster of neuroepithelial cells), postmitotic cells appeared first in the ganglion cell layer, then the inner nuclear layer, and then the outer nuclear layer, so cell birthday and cell fate were correlated within a column. The terminal mitoses occurred in three bursts separated by two 10-h intervals during which proliferation continued without terminal mitoses. (+info)
Anti-BrdU Antibody, clone BU-1 | 05-633
Protocol - Anti-BrdU Staining Using 70% Ethanol and 2N HCL
Cell Proliferation Kit II (BrdU) | PromoCell
Abnormal DNA synthesis in polyamine deficient cells revealed by bromodeoxyuridine-flow cytometry technique - Lund University
Anti-BrdU抗体[IIB5] (ab8955)参考文献| Abcam中国官方网站
BrdU Cell Proliferation ELISA Kit - Mitoproteins Mitochondrial Protein Partnership (MPP)
Technology - Biovica
Biophysical and computational approaches to unravel the molecular interaction mechanism of bromodeoxyuridine, a proliferative...
Mouse anti BrdU Antibody | Bio-Rad
www.keul.de - Produkte: Produkte Der Spezialist f r Kontrollblute Kontrollseren Diagnostika Laborgeräte Hamatologie...
BrdU Antibody (G3G4 (anti-BrdU)) - DSHB
Applications of BrdU Antibodies | Bio-Rad
Increased incorporation of 5-bromodeoxyuridine into the DNA of proliferating tissues in partially hepatectomised mice<...
Integrated DNA Technologies
Plus it
Pescadillo 293T transfected lysate (ab94282) | Abcam
Use of Bromodeoxyuridine to Study White Blood Cell Replication and Survival in HIV-Infected Patients - No Study Results Posted ...
Bromodeoxyuridine/BrdU Antibody (BU20a) [DyLight 550] (NBP2-34567R): Novus Biologicals
Bromodeoxyuridine/BrdU Antibody (BU20a) [DyLight 488] (NBP2-34567G): Novus Biologicals
Analysis of turnover of Treg by BrdU incorporation. BAL | Open-i
2-Bromo-4-fluorotoluene 98 % | 1422-53-3 | Sigma-Aldrich
8-Bromo-5-nitroisoquinoline | CAS No. 252861-41-9 | Sigma-Aldrich
5-bromo-2-4-fluorobenzyl-oxy-benzaldehyde | VWR
4-2-2-bromo-4-tert-pentyl-phenoxy-ethyl-piperidine-hydrochloride | VWR
I am ashamed to say I have done this more than once | Rebrn.com
Neuronal regeneration in a zebrafish model of adult brain injury | Disease Models & Mechanisms
Neuronal regeneration in a zebrafish model of adult brain injury | Disease Models & Mechanisms
Neuroscience2014 | Per session
Cell Cycle - IDA Yasam Teknolojileri
Anti-BrdU 抗体 (Biotin) (ab2284) | アブカム
Bromodeoxyuridine (BrdU) (Proliferation Marker) Antibody - With BSA and Azide - Mouse Monoclonal Antibody [Clone BRD469 +...
JCI -
LRIG1 inhibits STAT3-dependent inflammation to maintain corneal homeostasis
Problems with BrdU staining - Immunology and Histology
Cell Cycle Analysis | BrdU Incorporation in DNA | Monoclonal Anti-Bromodeoxyuridine (Clone BU6-4)
656-65-5, 4-Bromo-3-fluoroaniline, CAS No 656-65-5 4-Bromo-3-fluoroaniline pt
Alfa Aesar™ 2-Bromo-6-fluorobenzylamine hydrochloride, 96% 250mg Alfa Aesar™ 2-Bromo-6-fluorobenzylamine hydrochloride, 96%
3-bromo-6-(4-chlorophenyl)-1,2,4,5-tetraazine | C8H4BrClN4 - ChemSynthesis
The Retinoblastoma family member p107 regulates the rate of progenitor commitment to a neuronal fate | JCB
A Murine Model for Retinopathy of Prematurity Identifies Endothelial Cell Proliferation as a Potential Mechanism for Plus...
PI3K-Akt-Wnt Pathway Is Implicated in Exercise-Induced Improvement of Short-term Memory in Cerebral Palsy Rats
Localization and characterization of limbal T cells relative to label-retaining cells via immunofluorescence tomography | IOVS ...
Cell cycle analysis using flow cytometry<...
Plus it
High-speed photodamage cell selection using bromodeoxyuridine/Hoechst 33342 photosensitized cell killing - Herweijer - 1988 -...
Clinical outcome and bromodeoxyuridine derived proliferation indices in 100 colonic and rectal carcinomas - ePrints Soton
IDEALS @ Illinois: The development of a monoclonal antibody against 5-Bromodeoxyuridine and its applications in research...
The effect of late intrauterine 5-bromo-2-deoxyuridine administration on the development of GnRH neurons in the mouse |...
bromouracil - oi
Cell-cell Signaling by Direct Contact Increases Cell Proliferation via a PI3K-dependent Signal - PubMed
1-Bromo-3,3-dimethoxypropane supplier distributor- CAS 36255-44-4
TPOT: 自动化的sklearn - 技术博文 - 博学网
5-Bromo-4-chloro-3-indoxyl-α-D-fucopyranoside - Creative Enzymes
CAS # 160892-07-9, 5-Bromo-1,3-benzenedicarbonitrile, 5-Bromoisophthalonitrile
Macrostomum lignano
A bromodeoxyuridine analysis". 226: 231-241. Cite journal requires ,journal= (help) Rieger, R; Ladurner, P (2003). "The ...
Mutation
Induced mutations on the molecular level can be caused by: Chemicals Hydroxylamine Base analogs (e.g., Bromodeoxyuridine (BrdU ...
Harold M. Weintraub
The effects of bromodeoxyuridine on cell differentiation (conversion of a primitive cell into a more specialized cell) were ... Weintraub, H; Campbell, GL; Holtzer, H (1972). "Identification of a developmental program using bromodeoxyuridine". J Mol Biol ...
Single-cell DNA template strand sequencing
During S-phase cells are treated with bromodeoxyuridine (BrdU) which is then incorporated into their nascent DNA, acting as a ... LAMBERT, BO; HANSSON, KERSTIN; LINDSTEN, JAN; STEN, MARGARETA; WERELIUS, BARBRO (12 February 2009). "Bromodeoxyuridine-induced ...
5-Bromouridine
5-Bromouracil Bromodeoxyuridine 5-bromouridine in Linstrom, Peter J.; Mallard, William G. (eds.); NIST Chemistry WebBook, NIST ...
Lucy Graves Taliaferro
Effect of 5-bromodeoxyuridine on the hemolysin response in rabbits. Proc. Soc. Exp. Biol. Med., 124: 671-75. 1968 With W. H. ...
Marianne Frommer
... based on incorporation of bromodeoxyuridine into single-stranded probes. The method was used to localise satellite repeats very ... Localisation of satellite DNA sequences on human metaphase chromosomes using bromodeoxyuridine-labelled probes. Chromosoma 97: ...
5-Ethynyl-2'-deoxyuridine
Unlike the commonly used bromodeoxyuridine, EdU detection requires no heat or acid treatment. EdU incorporated into DNA induces ...
Wallace P. Rowe
High-Frequency Activation in vitro by 5-Iododeoxyuridine and 5-Bromodeoxyuridine". Science. 174 (4005): 155-156. Bibcode: ...
Oxalotrophic
"Identification of active oxalotrophic bacteria by Bromodeoxyuridine DNA labeling in a microcosm soil experiments". FEMS ...
Georg F. Striedter
... pulse-labeling chick embryos with bromodeoxyuridine". Journal of Neuroscience. 20 (21): 8021-8030. doi:10.1523/jneurosci.20-21- ...
Starfish regeneration
Moss, Claire; Jackie Hunter, A.; Thorndyke, Michael C. (1998). "Patterns of bromodeoxyuridine incorporation and neuropeptide ...
Androgen
Neural injections of Bromodeoxyuridine (BrdU) were applied to males of both groups to test for neurogenesis. Analysis showed ...
Thymidine kinase in clinical chemistry
There it is detected with an ELISA technique: The wells are filled with a solution of a monoclonal antibody to bromo-deoxyuridine ... The DiviTum assay from Biovica International uses another thymidine analogue, bromodeoxyuridine, as substrate to the enzyme. ...
Mutation
Base analogs (e.g., Bromodeoxyuridine (BrdU)). *Alkylating agents (e.g., N-ethyl-N-nitrosourea (ENU)). These agents can mutate ...
Zidovudine
"Induction of endogenous virus and of thymidine kinase by bromodeoxyuridine in cell cultures transformed by Friend virus". ...
Neoblast
This mitotic characteristic is how they are detected by adding Bromodeoxyuridine (BrdU) and staining with anti-BrdU. They have ...
Thymidine kinase
Merrick CJ (December 2015). "Transfection with thymidine kinase permits bromodeoxyuridine labelling of DNA replication in the ...
Thymidine
Bromodeoxyuridine (BrdU) is another thymidine analog that is often used for the detection of proliferating cells in living ...
Hoechst stain
Hoechst 33342 and 33258 are quenched by bromodeoxyuridine (BrdU), which is commonly used to detect dividing cells. Hoechst ...
Intracerebroventricular injection
"A comparative analysis of intraperitoneal versus intracerebroventricular administration of bromodeoxyuridine for the study of ...
Sister chromatid exchange
The Giemsa staining was able to stain due to the presence of bromodeoxyuridine analogous base which was introduced to the ...
Chromosome combing
... bromodeoxyuridine) into the nascent DNA, then fluorescently detecting it. As replication forks spread bidirectionally from ...
T-47D
... possibly due to inhibition by 5-bromodeoxyuridine and sodium butyrate. Yu, S; et al. (6 May 2017). "The T47D cell line is an ...
HAT medium
Thus, a cell lacking TK is resistant to bromodeoxyuridine (BrdU) and a cell lacking HGPRT is resistant to 6-thioguanine (6-TG) ...
Immortal DNA strand hypothesis
... or parental DNA strands with a DNA label such as tritiated thymidine or bromodeoxyuridine (BrdU). These types of DNA labels ...
Ethidium bromide
... or bromodeoxyuridine-induced carcinogenesis in laboratory stock]. Retrieved 2011-09-08. Kramer, M. J.; Grunberg, E. (1973). " ...
Chromosomal fragile site
... which are induced by bromodeoxyuridine (BrdU) or distamycin A, an antibiotic that preferentially binds to AT-pairs of DNA. The ...
Stem cell marker
... of ROSA26 mice beta1-integrin bromodeoxyuridine c-kit (CD117) c-Met C1qR(p) END (CD105) PROM1 (CD133) ALCAM (CD166) ITGB1 (CD29 ...
Renal stem cell
Using stain-retaining assay (with bromodeoxyuridine, or BrdU), a low-cycling cell population was found in the papillary region ...
Bromodeoxyuridine - Wikipedia
Bromodeoxyuridine releases gene silencing caused by DNA methylation. BrdU can also be used to identify microorganisms that ... Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU, BUdR, BrdUrd, broxuridine) is a synthetic nucleoside analogue with a chemical ... Dolbeare, F (May 1995). "Bromodeoxyuridine: a diagnostic tool in biology and medicine, Part I: Historical perspectives, ... "Correlation Between Bromodeoxyuridine- Labeling Indices and Patient Prognosis in Cerebral Astrocytic Tumors of Adults". Cancer ...
The effects of bromodeoxyuridine and bromouracil on regeneration in Hydra | SpringerLink
... bromouracil and bromodeoxyuridine, have been studied. Bromouracil inhibits tentacl ... The effects of bromodeoxyuridine and bromouracil on regeneration inHydra *M. H. Goyns1. & ... Goyns, M.H., Stanisstreet, M. The effects of bromodeoxyuridine and bromouracil on regeneration inHydra . W. Roux Archiv f. ... Lasher, R., Cahn, R. D.: Effects of 5-bromodeoxyuridine on the differentiation of chondrocytes in vitro. Develop. Biol.19, 415- ...
anti-Bromodeoxyuridine/BrdU, Biotin, Clone: SPM537, Novus Biologicals 100
| Fisher Scientific
anti-Bromodeoxyuridine/BrdU, Biotin, Clone: SPM537, Novus Biologicals 100 Tests; Biotin Life Sciences:Antibodies:Primary ... Bromodeoxyuridine/BrdU Monoclonal antibody specifically detects Bromodeoxyuridine/BrdU in All Species samples. It is validated ... It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU ...
Bromodeoxyuridine/BrdU Antibody (NB500-235): Novus Biologicals
Sheep Polyclonal Anti-Bromodeoxyuridine/BrdU Antibody Proliferation Marker cited in 15 publications. Validated: ICC/IF, IHC, ... Bromodeoxyuridine/BrdU Antibody Summary. Immunogen. Bromodeoxyuridine (BrdU) coupled to keyhole limpet hemocyanin (KLH). ... BrDU (Bromodeoxyuridine). The thymidine synthetic nucleoside analogue bromodeoxyuridine (BrdU) has a long, colorful history of ... Blogs on Bromodeoxyuridine/BrdU. Check out the latest blog posts on Bromodeoxyuridine/BrdU. ...
Tumor Radiosensitization by Sustained Intratumoral Release of Bromodeoxyuridine | Cancer Research
Tumor Radiosensitization by Sustained Intratumoral Release of Bromodeoxyuridine. Annie Doiron, Donald T. T. Yapp, Marina ... Tumor Radiosensitization by Sustained Intratumoral Release of Bromodeoxyuridine. Annie Doiron, Donald T. T. Yapp, Marina ... Tumor Radiosensitization by Sustained Intratumoral Release of Bromodeoxyuridine. Annie Doiron, Donald T. T. Yapp, Marina ... Bagshaw M. A., Doggett R. L., Smith K. C., Kaplan H. S., Nelsen T. S. Intra-arterial 5-bromodeoxyuridine and x-ray therapy. Am ...
Bromodeoxyuridine (BrdU) - OpenWetWare
Bromodeoxyuridine/BrdU Antibody (BRD494) [Biotin] (NBP2-34566B): Novus Biologicals
Mouse Monoclonal Anti-Bromodeoxyuridine/BrdU Antibody (BRD494) [Biotin]. Proliferation Marker. Validated: ICC/IF, IHC-Fr, IHC-P ... Check out the latest blog posts on Bromodeoxyuridine/BrdU.. BrDU (Bromodeoxyuridine). The thymidine synthetic nucleoside ... Bromodeoxyuridine/BrdU Antibody (BRD494) [Biotin] Summary. Immunogen. Bromodeoxyuridine (BrdU) conjugated to KLH ... Reviews for Bromodeoxyuridine/BrdU Antibody (NBP2-34566B) (0) There are no reviews for Bromodeoxyuridine/BrdU Antibody (NBP2- ...
High-speed photodamage cell selection using bromodeoxyuridine/Hoechst 33342 photosensitized cell killing - Herweijer - 1988 -...
HPLC/UV or HPLC/32P-postlabeling methods for quantification of bromodeoxyuridine in DNA | Cancer Research
HPLC/UV or HPLC/32P-postlabeling methods for quantification of bromodeoxyuridine in DNA. M. Vijayaraj Reddy, John E. Barnum, ... The extent of incorporation of exogenously administered bromodeoxyuridine (BrdU) into DNA is an indicator of the rate of DNA ... HPLC/UV or HPLC/32P-postlabeling methods for quantification of bromodeoxyuridine in DNA ... HPLC/UV or HPLC/32P-postlabeling methods for quantification of bromodeoxyuridine in DNA ...
Use of Bromodeoxyuridine to Study White Blood Cell Replication and Survival in HIV-Infected Patients - No Study Results Posted ...
Mouse Monoclonal anti-BrdU (Bromodeoxyuridine) (BRD469) | Biotium
Cell Cycle Analysis | BrdU Incorporation in DNA | Monoclonal Anti-Bromodeoxyuridine (Clone BU6-4)
Monoclonal Anti-Bromodeoxyuridine (Clone BU6-4). Monoclonal Anti-Bromodeoxyuridine (Clone BU6-4) is often used in cell cycle ... The monoclonal anti-bromodeoxyuridine antibody can be used in Western blot analysis under non-reducing and non-heating ... Immunogen: Bromodeoxyuridine-BSA conjugate *Immunologic host: BALB/c mouse *antibody subclass: IgG1 *Antibody titer: 1:2,000 ... analysis studies; the level of bromodeoxyuridine (BrdU) incorporation into DNA is a direct parameter of cell number and growth ...
Effects of bromodeoxyuridine, cytosine arabinoside and Colcemid upon in vitro development of mouse blastocysts | Development
Effects of bromodeoxyuridine, cytosine arabinoside and Colcemid upon in vitro development of mouse blastocysts ... Effects of bromodeoxyuridine, cytosine arabinoside and Colcemid upon in vitro development of mouse blastocysts ... Effects of bromodeoxyuridine, cytosine arabinoside and Colcemid upon in vitro development of mouse blastocysts ... Effects of bromodeoxyuridine, cytosine arabinoside and Colcemid upon in vitro development of mouse blastocysts ...
Biophysical and computational approaches to unravel the molecular interaction mechanism of bromodeoxyuridine, a proliferative...
Abstract The interaction of a nucleoside analogue bromodeoxyuridine (BrdU) with human serum albumin (HSA) was studied to ... Bromodeoxyuridine Human serum albumin Drug-protein interaction Fluorescence quenching Molecular modelling Electronic ... The interaction of a nucleoside analogue bromodeoxyuridine (BrdU) with human serum albumin (HSA) was studied to investigate the ... Biophysical and computational approaches to unravel the molecular interaction mechanism of bromodeoxyuridine, a proliferative ...
Isolation by restriction endonuclease digestion and base-specific affinity chromatography of rat-embryo DNA sequences...
Effect of 5-bromodeoxyuridine on the appearance of the liver isoenzyme of pyruvate kinase Biochem J (June, 1984) ... Control and bromodeoxyuridine-containing rat-embryo-cell DNA were digested by the restriction endonucleases Hpa II and Msp I ... base-specific affinity chromatography of rat-embryo DNA sequences disproportionately enriched in virogenic bromodeoxyuridine ... base-specific affinity chromatography of rat-embryo DNA sequences disproportionately enriched in virogenic bromodeoxyuridine. ...
Clinical outcome and bromodeoxyuridine derived proliferation indices in 100 colonic and rectal carcinomas - ePrints Soton
Cutress, R.I., Mullee, M.A. and Rew, D.A. (2002) Clinical outcome and bromodeoxyuridine derived proliferation indices in 100 ... Clinical outcome and bromodeoxyuridine derived proliferation indices in 100 colonic and rectal carcinomas ... Aim: In vivo labelling of human colonic and rectal tumours with bromodeoxyuridine (BrdUrd) and analysis by flow cytometry (FCM ... Aim: In vivo labelling of human colonic and rectal tumours with bromodeoxyuridine (BrdUrd) and analysis by flow cytometry (FCM ...
IDEALS @ Illinois: The development of a monoclonal antibody against 5-Bromodeoxyuridine and its applications in research...
The development of a monoclonal antibody against 5-Bromodeoxyuridine and its applications in research microfilm. Welcome to the ... The development of a monoclonal antibody against 5-Bromodeoxyuridine and its applications in research microfilm. Schmeda, Peter ... The development of a monoclonal antibody against 5-Bromodeoxyuridine and its applications in research microfilm. ...
Bioavailability of bromodeoxyuridine in dogs and toxicity in rats. - Semantic Scholar
Bioavailability of bromodeoxyuridine in dogs and toxicity in rats. by Surasak Phuphanich et al. ... A New Method for In Vitro Detection of Bromodeoxyuridine in Serum: A Proof of Concept in a Songbird Species, the Canary. * ... Bioavailability of bromodeoxyuridine in dogs and toxicity in rats.. @article{Phuphanich1985BioavailabilityOB, title={ ... Bioavailability of bromodeoxyuridine in dogs and toxicity in rats.}, author={Surasak Phuphanich and V. A. Levin}, journal={ ...
Bromodeoxyuridine (BrdU) (Proliferation Marker) Antibody - With BSA and Azide - Mouse Monoclonal Antibody [Clone BRD469 +...
Bromodeoxyuridine (BrdU) (Proliferation Marker) Antibody - With BSA and Azide, Mouse Monoclonal Antibody [Clone BRD469 + BRD494 ... Bromodeoxyuridine (BrdU) (Proliferation Marker) Antibody - With BSA and Azide is for research use only and not for use in ... It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU ... Bromodeoxyuridine (BrdU) (Proliferation Marker) Antibody - With BSA and Azide. Mouse Monoclonal Antibody [Clone BRD469 + BRD494 ...
Pairing properties of bromouracil and repair of bromouracil-containing DNA. Possible utilization of bromodeoxyuridine...
Biotinylated Bromodeoxyuridine Antibody - Biocare Medical
Biotinylated Bromodeoxyuridine Antibody recognizes (BrdU), an analog to thymidine and can be incorporated into replicating DNA ... Biotinylated Bromodeoxyuridine Antibody recognizes bromodeoxyuridine (BrdU), an analog to thymidine and can be incorporated ... Be the first to review "Biotinylated Bromodeoxyuridine (BrdU)" Cancel reply. You must be logged in to post a review. ...
Microbiology Society Journals | Inhibition of Sporulation in Bacillus subtilis by Bromodeoxyuridine and the Effect on DNA...
Cytochemistry for bromodeoxyuridine/DNA analysis: stoichiometry and sensitivity. - Semantic Scholar
... describes an improved immunochemical procedure for staining cells in suspension for amount of incorporated bromodeoxyuridine ( ... Improved bromodeoxyuridine/DNA analysis by anti-BudR monoclonal antibody versus right angle light scatter. Marco Vitale, Luca M ... Cytochemistry for bromodeoxyuridine/DNA analysis: stoichiometry and sensitivity.. @article{Dolbeare1985CytochemistryFB, title={ ... Cytochemistry for bromodeoxyuridine/DNA analysis: stoichiometry and sensitivity.}, author={Frank Dolbeare and Wolfgang Beisker ...
Mouse anti BrdU (bromodeoxyuridine) - Exalpha Biologicals inc.
Mouse anti BrdU (bromodeoxyuridine). Catalogue number: X1028. Synonyms. Anti-Bromodeoxyuridine Mouse MoBU Monoclonal Antibody ... Bromodeoxyuridine conjugated to Helix Pomatia Haemocyanin used to immunize BALB/c mice Purification Method. Protein A/G ... Application of bromodeoxyuridine (BrdU) and anti-BrdU monoclonal antibody for the in vivo analysis of proliferative ... Oncology 1991, 48, 285-289 2. Wilson, G., Cell kinetic studies using a monoclonal antibody to bromodeoxyuridine. Methods Mol. ...
Abnormal DNA synthesis in polyamine deficient cells revealed by bromodeoxyuridine-flow cytometry technique - Lund University
Mixed lymphocyte reactions evaluated by means of bromodeoxyuridine incorporation<...
Mixed lymphocyte reactions evaluated by means of bromodeoxyuridine incorporation. A. Bontadini, R. Conte, A. Dinota, D. ... Mixed lymphocyte reactions evaluated by means of bromodeoxyuridine incorporation. / Bontadini, A.; Conte, R.; Dinota, A.; ... title = "Mixed lymphocyte reactions evaluated by means of bromodeoxyuridine incorporation",. author = "A. Bontadini and R. ... Mixed lymphocyte reactions evaluated by means of bromodeoxyuridine incorporation. Haematologica. 1990;75(1):7-11. ...
Disruption of hippocampal development in vivo by CR-50 mAb against Reelin | PNAS
5-bromodeoxyuridine;. En,. embryonic day n;. FITC,. fluorescein isothiocyanate;. Pn,. postnatal day n;. MAP2,. microtubule- ... 5-Bromodeoxyuridine (BrdUrd) Labeling.. Pregnant mice were injected intraperitoneally with BrdUrd (Sigma; 10 mg/ml in PBS) at ... Labeling experiments with 5-bromodeoxyuridine demonstrated that the labeled cells in the stratum pyramidale of the CR-50- ...
Overlapping and Divergent Actions of Estrogen and the Neurotrophins on Cell Fate and p53-Dependent Signal Transduction in...
ANTI - BROMODEOXYURIDINE, MONOCLONAL (0811202) - MP Biomedicals
Iodo- and bromodeoxyuridine are excised at different rates from DNA of mouse tongue keratinocytes in vitro.
Antibody11
- Bromodeoxyuridine/BrdU Monoclonal antibody specifically detects Bromodeoxyuridine/BrdU in All Species samples. (fishersci.com)
- Immunocytochemistry/ Immunofluorescence: Bromodeoxyuridine/BrdU Antibody [NB500-235] - IF analysis of BrdU in Proteinase K pretreated mouse lacrimal gland. (novusbio.com)
- There are currently no images for Bromodeoxyuridine/BrdU Antibody (NBP2-34566B). (novusbio.com)
- The monoclonal anti-bromodeoxyuridine antibody can be used in Western blot analysis under non-reducing and non-heating conditions and in flow cytometry applications. (clontech.com)
- Biotinylated Bromodeoxyuridine Antibody recognizes bromodeoxyuridine (BrdU), an analog to thymidine and can be incorporated into replicating DNA during the S-phase of the cell cycle. (biocare.net)
- Application of bromodeoxyuridine (BrdU) and anti-BrdU monoclonal antibody for the in vivo analysis of proliferative characteristics of human leukemia cells in bone marrows. (exalpha.com)
- Wilson, G., Cell kinetic studies using a monoclonal antibody to bromodeoxyuridine. (exalpha.com)
- A monoclonal antibody to bromodeoxyuridine (BrdUrd) incorporated into DNA allowed visualization of sister chromatid exchanges (SCE) when as little as 0.6% of the thymine in a single DNA strand has been substituted. (elsevier.com)
- In vitro immunohistochemical localization of S-phase cells by a monoclonal antibody to bromodeoxyuridine. (core.ac.uk)
- First, the use of 3 H-thymidine, a radioactive nucleotide used to study proliferation when incorporated into the cells during the S phase of the cell cycle, was replaced by its analog, bromodeoxyuridine (BrdU), which could be detected by a specific antibody. (pubmedcentralcanada.ca)
- Thymidine (Tdr) and its analogues 5-fluorodeoxyuridine (FUdr) and 5-bromodeoxyuridine (BUdr) inhibited the development of antibody-producing cells (PFC) in cultures of explants of rabbit spleen stimulated with sheep red cells in vitro. (biomedsearch.com)
BUdR3
- Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd, broxuridine) is a synthetic nucleoside analogue with a chemical structure similar to thymidine. (wikipedia.org)
- This study includes 182 patients with intracranial gliomas who received bromodeoxyuridine (BUdR), 200 mg/sq m intravenously, at the time of craniotomy but before tumor biopsy. (thejns.org)
- One hundred fifty-two intracranial gliomas of various types were reviewed in order to correlate the histopathological features with the proliferative potential of each tumor as reflected by the bromodeoxyuridine (BUdR) labeling index (LI). (thejns.org)
BrdUrd4
- We have now investigated, in the same tumor model, radiosensitization by the thymidine analogue bromodeoxyuridine (BrdUrd). (aacrjournals.org)
- Aim: In vivo labelling of human colonic and rectal tumours with bromodeoxyuridine (BrdUrd) and analysis by flow cytometry (FCM) allows the labelling index (LI), S phase duration (Ts) and the potential doubling time (Tpot) of the tumour to be estimated in vivo. (soton.ac.uk)
- This report describes an improved immunochemical procedure for staining cells in suspension for amount of incorporated bromodeoxyuridine (BrdUrd) and total DNA. (semanticscholar.org)
- At 1-4 days after seeding, the cells were labelled for 15-120 min with the thymidine analogue bromodeoxyuridine (BrdUrd) and they were then fixed directly after the labelling period. (lu.se)
Incorporation6
- The extent of incorporation of exogenously administered bromodeoxyuridine (BrdU) into DNA is an indicator of the rate of DNA synthesis and cell proliferation. (aacrjournals.org)
- the level of bromodeoxyuridine (BrdU) incorporation into DNA is a direct parameter of cell number and growth. (clontech.com)
- Gobbi, M. / Mixed lymphocyte reactions evaluated by means of bromodeoxyuridine incorporation . (elsevier.com)
- Hill, BT , Augenlicht, LH & Baserga, R 1973, ' Increased incorporation of 5-bromodeoxyuridine into the DNA of proliferating tissues in partially hepatectomised mice ', FEBS Letters , vol. 37, no. 2, pp. 298-302. (elsevier.com)
- Cell proliferation was assayed by cell counts and bromodeoxyuridine (BrdU) Incorporation. (molvis.org)
- In vivo treatment with PTN (20 ng/g) increased bromodeoxyuridine incorporation (by 2.24-fold) and PCNA expression (by 1.71-fold) during day 7 to day 14, indicating that PTN induces cell proliferation in mouse heart. (pubmedcentralcanada.ca)
Coupled to keyhole limpet hem1
- Bromodeoxyuridine (BrdU) coupled to keyhole limpet hemocyanin (KLH). (novusbio.com)
Synthetic nucleoside analogue2
- Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU ) is a synthetic nucleoside analogue of thymidine. (openwetware.org)
- The thymidine synthetic nucleoside analogue bromodeoxyuridine (BrdU) has a long, colorful history of repeated use in molecular and cytokinetic studies, as detailed in reviews by Vanderlaan and Dolbeare (1,2). (novusbio.com)
Nucleoside2
- BrdU (Bromodeoxyuridine) is a synthetic nucleoside that is incorporated into actively replicating cells' DNA. (novusbio.com)
- The interaction of a nucleoside analogue bromodeoxyuridine (BrdU) with human serum albumin (HSA) was studied to investigate the binding phenomenon and analyse the protein conformation upon BrdU binding. (springer.com)
Effects of bromodeoxyuridine1
- The effects of bromodeoxyuridine on cell differentiation (conversion of a primitive cell into a more specialized cell) were also analyzed. (wikipedia.org)
Mechanism of bromodeoxyuridine1
- To elucidate the mechanism of bromodeoxyuridine (BrdU) induced cellular senescence, we treated HeLa cells with D4476, a potent and specific inhibitor of casein kinase 1(CK1). (bio-journal.com)
Marker2
- Rats received repeated intraperitoneal injections of the cell proliferation-specific marker 5-bromodeoxyuridine (BrdU) after stroke induction. (ahajournals.org)
- Bromodeoxyuridine, an analogue of thymidine, can be detected by means of monoclonal antibodies and utilized as a marker of the S-phase of the cell cycle. (core.ac.uk)
Immunohistochemistry1
- The distribution of DNA synthesising cells in the crypts of the epithelium in human small and large bowel after injection of bromodeoxyuridine into patients has been studied in relation to the position of the cells in the crypt using immunohistochemistry. (openrepository.com)
Analogue1
- Coleman, J. R., Coleman, W. A., Hartline, E. J. H.: A clonal study of the reversible inhibition of muscle differentiation by the halogenated thymidine analogue 5-bromodeoxyuridine. (springer.com)
Antibodies2
- Gray, J. (Ed), Special Issue: Monoclonal antibodies against bromodeoxyuridine Cytometry 1985, Vol. 6(6) 4. (exalpha.com)
- The development of antibodies to iododeoxyuridine (IdU) and bromodeoxyuridine (BrdU) has increased interest in the use of these compounds in cell biology. (openrepository.com)
Reacts2
- It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. (fishersci.com)
- This product reacts specifically with bromodeoxyuridine. (clontech.com)
Cells4
- Labeling experiments with 5-bromodeoxyuridine demonstrated that the labeled cells in the stratum pyramidale of the CR-50-treated mice were distributed in a pattern similar to that of reeler . (pnas.org)
- Bromodeoxyuridine (BrdU) is a thymidine analog and it incorporates into the DNA only if the cells are at S-phase. (researchsquare.com)
- To investigate the optimal dosage and timing for 5-bromodeoxyuridine ( BrdU ) labeling of endothelial progenitor cells (EPCs) from rat circulating blood . (bvsalud.org)
- The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. (abcam.com)
Indices1
- Cutress, R.I. , Mullee, M.A. and Rew, D.A. (2002) Clinical outcome and bromodeoxyuridine derived proliferation indices in 100 colonic and rectal carcinomas. (soton.ac.uk)
Bromouracil1
- The effects on tentacle regeneration in Hydra of two DNA-antimetabolites, bromouracil and bromodeoxyuridine, have been studied. (springer.com)
Toxicity1
- Bioavailability of bromodeoxyuridine in dogs and toxicity in rats. (semanticscholar.org)
Replication1
- Replication not inhibited by 5-bromodeoxyuridine. (cdc.gov)
Cell4
- Holtzer, H.: Cell differentiation in the presence of bromodeoxyuridine. (springer.com)
- Control and bromodeoxyuridine-containing rat-embryo-cell DNA were digested by the restriction endonucleases Hpa II and Msp I and were subsequently analyzed by agarose-gel electrophoresis as well as DNA-affinity chromatography. (portlandpress.com)
- The labeling kinetics of 5 dendritic cell (DC) subtypes within the lymphoid organs of healthy laboratory mice during continuous administration of bromodeoxyuridine (BrdU) was determined to investigate developmental relationships and determine turnover rates. (bloodjournal.org)
- Morphometric measurements and measurements of cell proliferation by bromodeoxyuridine index analysis were performed at both arterial and venous anastomoses. (ahajournals.org)
Sensitivity1
- Cytochemistry for bromodeoxyuridine/DNA analysis: stoichiometry and sensitivity. (semanticscholar.org)
Differentiation1
- Lasher, R., Cahn, R. D.: Effects of 5-bromodeoxyuridine on the differentiation of chondrocytes in vitro. (springer.com)
Vivo2
- Proliferation in human gastrointestinal epithelium using bromodeoxyuridine in vivo: data for different sites, proximity to a tumour, and polyposis coli. (openrepository.com)
- Measurement of in vivo proliferation in human colorectal mucosa using bromodeoxyuridine. (openrepository.com)
Gene1
- Bromodeoxyuridine releases gene silencing caused by DNA methylation. (wikipedia.org)
Growth2
- An elevated bromodeoxyuridine labeling index is suggestive of a rapid growth rate of medulloblastoma. (wikidoc.org)
- An elevated bromodeoxyuridine labeling index is suggestive of a rapid growth rate of meningioma and a greater incidence of recurrence following surgical resection. (wikidoc.org)
Vitro1
- Iodo- and bromodeoxyuridine are excised at different rates from DNA of mouse tongue keratinocytes in vitro. (openrepository.com)