5-Bromo-2'-deoxycytidine. Can be incorporated into DNA in the presence of DNA polymerase, replacing dCTP.
A purine or pyrimidine base bonded to DEOXYRIBOSE.
Salts of hydrobromic acid, HBr, with the bromine atom in the 1- oxidation state. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A terminal section of a chromosome which has a specialized structure and which is involved in chromosomal replication and stability. Its length is believed to be a few hundred base pairs.
Tumors whose cells possess secretory granules and originate from the neuroectoderm, i.e., the cells of the ectoblast or epiblast that program the neuroendocrine system. Common properties across most neuroendocrine tumors include ectopic hormone production (often via APUD CELLS), the presence of tumor-associated antigens, and isozyme composition.
Proteins that specifically bind to TELOMERES. Proteins in this class include those that perform functions such as telomere capping, telomere maintenance and telomere stabilization.
An essential ribonucleoprotein reverse transcriptase that adds telomeric DNA to the ends of eukaryotic CHROMOSOMES.
Maintenance of TELOMERE length. During DNA REPLICATION, chromosome ends loose some of their telomere sequence (TELOMERE SHORTENING.) Various cellular mechanism are involved in repairing, extending, and recapping the telomere ends.
A ubiquitously expressed telomere-binding protein that is present at TELOMERES throughout the cell cycle. It is a suppressor of telomere elongation and may be involved in stabilization of telomere length. It is structurally different from TELOMERIC REPEAT BINDING PROTEIN 1 in that it contains basic N-terminal amino acid residues.
A ubiquitously expressed telomere-binding protein that is present at TELOMERES throughout the CELL CYCLE. It is a suppressor of telomere elongation and may be involved in stabilization of telomere length. It is structurally different from TELOMERIC REPEAT BINDING PROTEIN 2 in that it contains acidic N-terminal amino acid residues.
All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.
A publication issued at stated, more or less regular, intervals.
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
Lists of persons or organizations, systematically arranged, usually in alphabetic or classed order, giving address, affiliations, etc., for individuals, and giving address, officers, functions, and similar data for organizations. (ALA Glossary of Library and Information Science, 1983)
"The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.
Publications in any medium issued in successive parts bearing numerical or chronological designations and intended to be continued indefinitely. (ALA Glossary of Library and Information Science, 1983, p203)
Drugs used to potentiate the effectiveness of radiation therapy in destroying unwanted cells.
Naphthalene rings which contain two ketone moieties in any position. They can be substituted in any position except at the ketone groups.
The reconstruction of a continuous two-stranded DNA molecule without mismatch from a molecule which contained damaged regions. The major repair mechanisms are excision repair, in which defective regions in one strand are excised and resynthesized using the complementary base pairing information in the intact strand; photoreactivation repair, in which the lethal and mutagenic effects of ultraviolet light are eliminated; and post-replication repair, in which the primary lesions are not repaired, but the gaps in one daughter duplex are filled in by incorporation of portions of the other (undamaged) daughter duplex. Excision repair and post-replication repair are sometimes referred to as "dark repair" because they do not require light.
A nitroimidazole that sensitizes normally radio-resistant hypoxic cells to radiation. It may also be directly cytotoxic to hypoxic cells and has been proposed as an antineoplastic.
Surgical treatment of pulmonary tuberculosis whereby the lung is totally or partially, temporarily or permanently, immobilized. The procedure was based on the popular concept that collapsing the affected portion of a tuberculous lung allowed the infected area to rest and thereby recover. At the beginning of the 20th century artificially induced pneumothorax (PNEUMOTHORAX, ARTIFICIAL) was popular. Later a variety of other techniques was used to encourage collapse of the infected portion of the lung: unilateral phrenic nerve division, PNEUMONOLYSIS, pneumoperitoneum (PNEUMOPERITONEUM, ARTIFICIAL), and THORACOPLASTY. Collapse therapy has declined since the advent of antitubercular chemotherapy. (Stedman, 25th ed; from Sabiston Jr, Textbook of Surgery, 14th ed, p1733-4)
A series of heterocyclic compounds that are variously substituted in nature and are known also as purine bases. They include ADENINE and GUANINE, constituents of nucleic acids, as well as many alkaloids such as CAFFEINE and THEOPHYLLINE. Uric acid is the metabolic end product of purine metabolism.
Inorganic compounds that contain calcium as an integral part of the molecule.
The generic term for salts derived from silica or the silicic acids. They contain silicon, oxygen, and one or more metals, and may contain hydrogen. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th Ed)
A dideoxynucleoside compound in which the 3'-hydroxy group on the sugar moiety has been replaced by a hydrogen. This modification prevents the formation of phosphodiester linkages which are needed for the completion of nucleic acid chains. The compound is a potent inhibitor of HIV replication at low concentrations, acting as a chain-terminator of viral DNA by binding to reverse transcriptase. Its principal toxic side effect is axonal degeneration resulting in peripheral neuropathy.
Substances that inhibit or prevent the proliferation of NEOPLASMS.
The relationship between the dose of an administered drug and the response of the organism to the drug.
Diseases of the peripheral nerves external to the brain and spinal cord, which includes diseases of the nerve roots, ganglia, plexi, autonomic nerves, sensory nerves, and motor nerves.
A dideoxynucleoside compound in which the 3'-hydroxy group on the sugar moiety has been replaced by an azido group. This modification prevents the formation of phosphodiester linkages which are needed for the completion of nucleic acid chains. The compound is a potent inhibitor of HIV replication, acting as a chain-terminator of viral DNA during reverse transcription. It improves immunologic function, partially reverses the HIV-induced neurological dysfunction, and improves certain other clinical abnormalities associated with AIDS. Its principal toxic effect is dose-dependent suppression of bone marrow, resulting in anemia and leukopenia.
A reverse transcriptase encoded by the POL GENE of HIV. It is a heterodimer of 66 kDa and 51 kDa subunits that are derived from a common precursor protein. The heterodimer also includes an RNAse H activity (RIBONUCLEASE H, HUMAN IMMUNODEFICIENCY VIRUS) that plays an essential role the viral replication process.
An independent Federal agency established in 1958. It conducts research for the solution of problems of flight within and outside the Earth's atmosphere and develops, constructs, tests, and operates aeronautical and space vehicles. (From U.S. Government Manual, 1993)
An agency of the NATIONAL INSTITUTES OF HEALTH concerned with overall planning, promoting, and administering programs pertaining to advancement of medical and related sciences. Major activities of this institute include the collection, dissemination, and exchange of information important to the progress of medicine and health, research in medical informatics and support for medical library development.
Any woven or knit material of open texture used in surgery for the repair, reconstruction, or substitution of tissue. The mesh is usually a synthetic fabric made of various polymers. It is occasionally made of metal.
Propylene or propene polymers. Thermoplastics that can be extruded into fibers, films or solid forms. They are used as a copolymer in plastics, especially polyethylene. The fibers are used for fabrics, filters and surgical sutures.
A computerized biomedical bibliographic storage and retrieval system operated by the NATIONAL LIBRARY OF MEDICINE. MEDLARS stands for Medical Literature Analysis and Retrieval System, which was first introduced in 1964 and evolved into an online system in 1971 called MEDLINE (MEDLARS Online). As other online databases were developed, MEDLARS became the name of the entire NLM information system while MEDLINE became the name of the premier database. MEDLARS was used to produce the former printed Cumulated Index Medicus, and the printed monthly Index Medicus, until that publication ceased in December 2004.

Radiosensitization of rat glioma with bromodeoxycytidine and adenovirus expressing herpes simplex virus-thymidine kinase delivered by slow, rate-controlled positive pressure infusion. (1/9)

Infection of rat RT2 glioma cells in vitro with an adenovirus (ADV-TK) expressing herpes simplex virus (HSV) thymidine kinase (TK) and subsequent exposure to 5-bromo-2'-deoxycytidine (BrdC), which is specifically incorporated into ADV-TK-infected cell DNA as 5-bromo-2'-deoxyuridine (BrdU), results in significant radiosensitization (sensitizer enhancement ratio: 1.4-2.3) compared with Ad beta gal-infected cells. Cell killing correlated well with increased BrdU DNA incorporation and with apoptosis. Whereas radiation (4 Gy) alone was relatively ineffective in inducing apoptosis, treatment with HSV-TK/BrdC resulted in BrdC dose- (10-100 microM) and time-dependent (24-48 hours) increases, and the combination of the two treatments produced a synergistic response (1.5- to 2-fold). To investigate the effects of the ADV-TK/BrdC treatment in vivo, RT2 cells were grown as soft tissue tumors in Fischer 344 rats and conditions for virus infusion were optimized by altering the volume and rate of infusion using a rate-controlled positive pressure device. We found that relatively large volumes (100-150 microL) of virus delivered at rates of < or = 1 microL/minute were optimal and gave uniform and reproducible results. Using these optimal infusion conditions, we were able to achieve 40% adenovirus infection in the tumor. Infection of RT2 tumors with ADV-TK and continuous administration of BrdC from an osmotic pump resulted in significant (.001 < P < .009) tumor regression 6 days after radiation (30 Gy delivered as 2 x 5 Gy over 3 days) compared with controls. In situ staining of sectioned tumors with anti-BrdU antibody or by high-performance liquid chromatography analysis of extracted and hydrolyzed tumor DNA confirmed that we obtained efficient and specific incorporation of BrdU into tumor cells. These results suggest that adenovirus-mediated delivery of HSV-TK in combination with BrdC and radiation can potentially be an efficient combination modality for the treatment of gliomas.  (+info)

Sequence-dependent formation of intrastrand crosslink products from the UVB irradiation of duplex DNA containing a 5-bromo-2'-deoxyuridine or 5-bromo-2'-deoxycytidine. (2/9)

The replacement of thymidine with 5-bromo-2'-deoxyuridine (BrdU) is well-known to sensitize cells to ionizing radiation and photoirradiation. We reported here the sequence-dependent formation of intrastrand crosslink products from the UVB irradiation of duplex oligodeoxynucleotides harboring a BrdU or its closely related 5-bromo-2'-deoxycytidine (BrdC). Our results showed that two types of crosslink products could be induced from d(BrCG), d(BrUG), d(GBrU), or d(ABrU); the C(5) of cytosine or uracil could be covalently bonded to the N(2) or C(8) of its neighboring guanine, and the C(5) of uracil could couple with the C(2) or C(8) of its neighboring adenine. By using those crosslink product-bearing dinucleoside monophosphates as standards, we demonstrated, by using liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS), that all the crosslink products described above except d(G[N(2)-5]U) and d(G[N(2)-5]C) could form in duplex DNA. In addition, LC-MS/MS quantification results revealed that both the nature of the halogenated pyrimidine base and its 5' flanking nucleoside affected markedly the generation of intrastrand crosslink products. The yields of crosslink products were much higher while the 5' neighboring nucleoside was a dG than while it was a dA, and BrdC induced the formation of crosslink products much more efficiently than BrdU. The formation of intrastrand crosslink products from these halopyrimidines in duplex DNA may account for the photosensitizing effects of these nucleosides.  (+info)

Enzymatic basis for the selective inhibition of varicella-zoster virus by 5-halogenated analogues of deoxycytidine. (3/9)

5-Bromodeoxycytidine (BrdC) and 5-iododeoxycytidine, at a concentration of 100 mug/ml, effectively inhibit the replication of varicella-zoster (VZ) virus in tissue culture. No toxicity could be demonstrated in uninfected cells under the same conditions. Studies on the enzymatic basis for this selective inhibition were undertaken. Infection of human embryonic lung cell monolayers with VZ virus-infected cells results in the induction of thymidine (dT), deoxycytidine (dC), and BrdC kinase activities (which are increased 10-, 40-, and 60-fold, respectively) and in a 70-fold stimulation in the incorporation of 3H nucleotide (5-bromodeoxyuridylate) derived from BrdC into DNA. The thermal stability of the VZ virus-induced activities differs significantly from the activities induced by herpes simplex virus type 1 and herpes simplex virus type 2 and those present in uninfected human embryonic lung cells. The VZ virus-induced dT, dC, and BrdC kinase are similarly affected by temperature and cofractionate upon Sephadex gel filtration, findings consistent with the hypothesis that these activities are the function of a single enzyme: a pyrimidine deoxyribonucleoside kinase. The molecular weight, calculated on the basis of the elution pattern on Sephadex G-150, is 70,000. Kinetic studies, demonstrating that dT and dC competively inhibit the phosphorylation of BrdC, are consistent with the phosphorylation of these substrates at a common active site. Kinetic parameters include: KidT = 0.6 MUM; KidC = 60 muM; KmBrdC = 8.5 muM. In contrast to its relatively high affinity for the VZ virus-induced kinase, BrdC is a relatively poor substrate for the host kinases. Therefore, the basis for the selective inhibition of VZ virus by 5-halogenated analogues of dC is reflected in the induction of a pyrimidine deoxyribonucleoside kinase with a high affinity for BrdC.  (+info)

Murine mammary FM3A carcinoma cells transformed with the herpes simplex virus type 1 thymidine kinase gene are highly sensitive to the growth-inhibitory properties of (E)-5-(2-bromovinyl)-2'-deoxyuridine and related compounds. (4/9)

Murine mammary carcinoma (FM3A TK-/HSV-1 TK+) cells, which are thymidine kinase (TK)-deficient but have been transformed with the herpes simplex virus type 1 (HSV-1) TK gene are inhibited in their growth by (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVDU), (E)-5-(2-iodovinyl)-2'-deoxyuridine (IVDU) and (E)-5-(2-bromovinyl)-2'-deoxycytidine (BVDC) at 0.5, 0.5 and 0.8 ng/ml, respectively; i.e., a concentration 5000 to 20 000-fold lower than that required to inhibit the growth of the corresponding wild-type FM3A/0 cells. Hence, transformation of tumor cells with the HSV-1 TK gene makes them particularly sensitive to the cytostatic action of BVDU and related compounds.  (+info)

Potentiation of halogenated pyrimidine radiosensitizers in human carcinoma cells by beta-lapachone (3,4-dihydro-2,2-dimethyl-2H-naphtho[1,2-b]pyran- 5,6-dione), a novel DNA repair inhibitor. (5/9)

3,4-Dihydro-2,2-dimethyl-2H-naptho[1,2,-b]pyran-5,6-dione (beta-lapachone) is a novel DNA repair inhibitor. It was tested for synergistic X-ray-induced lethality in combination with several halogenated pyrimidine radiosensitizers. Logarithmic-phase growing human epidermoid laryngeal carcinoma (HEp-2) cells were allowed to incorporate pyrimidine analogues for 48 h (approximately two cell doublings) and then were X-irradiated and subjected to various posttreatments. beta-Lapachone synergistically increased the dose enhancement ratios (DERs) of all analogues screened, with the exception of the 2'-chloro derivative of 5-bromodeoxyuridine. For example, following 5-bromodeoxycytidine sensitization an X-ray DER value of 1.87 +/- 0.04 at 1% survival was increased to 3.51 +/- 0.42 due to a 4-h post-X-irradiation exposure to 4 microM beta-lapachone. Do and Dq values for halogenated pyrimidine-sensitized human epidermoid laryngeal carcinoma cells were decreased 1.4- to 5.4-fold and 1.4- to 4.0-fold, respectively. beta-Lapachone had little effect upon the cytotoxicities of unirradiated human epidermoid laryngeal carcinoma cells whether or not they were previously exposed to any of the halogenated pyrimidine radiosensitizers. beta-Lapachone treatment following X-irradiation of cells that had not incorporated a pyrimidine analogue exhibited DER values of 1.38 +/- 0.05 and 1.40 +/- 0.01 at 10 and 1% survival levels, respectively. beta-Lapachone enhanced the radiosensitization of deoxycytidine analogues to a greater extent than the structurally related deoxyuridine analogues. Greater DERs and lower Do and Dq values were found for deoxycytidine than for deoxyuridine analogue radiosensitizers following beta-lapachone treatment. This agent may improve presently used radiation therapies and enhance proposed strategies which utilize deoxycytidine analogue radiosensitization together with protection of normal tissues by tetrahydrouridine to achieve tumor-selective radiotherapy.  (+info)

Incorporation of 5-bromodeoxycytidine in the adenovirus 2 replication origin interferes with nuclear factor 1 binding. (6/9)

We have studied the binding of nuclear factor 1 (NFI), a human sequence-specific DNA-binding protein, to a DNA fragment substituted in vitro with 5-bromodeoxycytidine (5-BrdC). Even at low substitution grades binding of NFI to its recognition sequence was considerably lower than with the unsubstituted control fragment. We developed a procedure to cleave substituted DNA specifically at a BrdC residue and searched for contacts between NFI and 5-BrdC residues by an interference assay. Surprisingly, no specific contacts were found in or near the recognition sequence. It appeared instead that interference was inversely related to the distance of a 5-BrdC residue from the NFI binding site. Models to explain these results, including a possible sliding mechanism, are discussed.  (+info)

Hemimethylated duplex DNAs prepared from 5-azacytidine-treated cells. (7/9)

Duplex heavy-light (HL) DNAs synthesized in the presence of brdUrd and methylation inhibitors were separated from bulk cellular DNA by CsCl density gradient centrifugation and analysed for 5-methylcytosine (5mC) contents by HPLC. DNAs synthesized in the presence of 5 mM ethionine or 2 mg/ml cycloleucine were not detectably hypomethylated, was undermethylated with respect to control DNA. The heavy, or H-strand, in which up to 5% of the cytosine residues were replaced by intact 5-azacytosine, was undermethylated and the HL duplex DNA was therefore strand asymmetrically methylated. This duplex DNA served as an efficient substrate for a crude DNA methyltransferase preparation which transferred the methyl group from S-adenosylmethionine specifically into cytosine residues within the hypomethylated H strand. Increasing levels of incorporated 5-azacytosine inhibited the action of the methyltransferase suggesting that incorporation of 5-azacytosine into DNA may be responsible for the inhibitory effect of 5-azacytidine on DNA methylation.  (+info)

Heritable fragile sites on human chromosomes. XI. Factors affecting expression of fragile sites at 10q25, 16q22, and 17p12. (8/9)

The fragile sites at 10q25, 16q22, and 17p12 can all be induced in lymphocyte culture by BrdU or BrdC added 6-12 hrs prior to harvest. Without induction, fra(10)(q25) is rarely expressed spontaneously, whereas fra(16)(q22) is frequently expressed spontaneously. Fra(17)(p12) is frequently expressed spontaneously but is probably expressed only after induction in some individuals. Distamycin A, netropsin, and Hoechst 33258 induced high levels of expression of fra(16)(q22) and fra(17)(p12) but did not enhance expression of fra(10)(q25). The mechanisms of induction of fra(16)(q22) by BrdU and distamycin A appear to be different, since the time of induction by BrdU reaches a maximum about 12 hrs prior to harvest whereas induction by distamycin A requires much longer exposure. The fragile sites at 10q25 and 16q22 were both induced in fibroblast culture by BrdU. Fra(17)(p12) is accepted as a fragile site because preliminary studies show that it behaves similarly in lymphocyte culture to fra(16)(q22); however, there is only limited evidence for fragility at 17p12.  (+info)

TY - JOUR. T1 - Potentiation of halogenated pyrimidine radiosensitizers in human carcinoma cells by β-lapachone (3,4-dihydro-2,2-dimethyl-2H-naphtho[1,2-b]pyran-5,6-dione), a novel DNA repair inhibitor. AU - Boothman, D. A.. AU - Greer, S.. AU - Pardee, A. B.. PY - 1987. Y1 - 1987. N2 - 3,4-Dihydro-2,2-dimethyl-2H-naptho[1,2-b]pyran-5,6-dione (β-lapachone) is a novel DNA repair inhibitor. It was tested for synergistic X-ray-induced lethality in combination with several halogenate pyrimidine radiosensitizers. Logarithmic-phase growing human epidermoid laryngeal carcinoma (HEp-2) cells were allowed to incorporate pyrimidine analogues for 48 h (approximately two cell doublings) and then were X-irradiated and subjected to various posttreatments. β-Lapachone synergistically increase the dose enhancement ratios (DERs) of all analogues screened, with the exception of the 2-chloro derivative of 5-bromodeoxyuridine. For example, following 5-bromodeoxycytidine sensitization an X-ray DER value of 1.87 ...
Although phase I clinical trials have shown that DNA repair inhibitors may represent powerful and specific anticancer treatments, reactivating mutations in targeted pathways will likely lead to therapeutic resistance (3, 27-29), making the discovery of additional synthetically lethal interactions in DNA repair pathways critically important for the ultimate success of this emerging class of drugs. The platform described here provides an opportunity to accelerate the development of potential drug targets in the DNA repair network by allowing the simultaneous (i) discovery of targetable and functional motifs, (ii) prioritization of targets by functional interaction, and (iii) unbiased exploration for proteins with unrecognized roles in DNA repair.. To validate the utility of this approach, we selected GNFRYLAPP as a lead candidate over other promising peptides returned in the screen. The basis for this choice was first that the peptide mimics a small protein, increasing the odds that an ...
Irrigation during intraventricular endoscopic surgery is critical for visualization, with normal intracranial pressure maintained by balancing fluid ingress and egress. Although irrigation is typically achieved through manual manipulation of inexact stopcocks, the authors have developed a rate-controlled, foot pedal-activated system for precise intraventricular irrigation by using a standard irrigating bipolar electrocautery machine.. This study is a retrospective review of patients who underwent endoscopic intraventricular surgery between January 1, 2018, and September 25, 2019, in which this irrigation system was used. Important components of this system include a bipolar module irrigation regulator that is set to a desired rate, a secure connection of the bipolar irrigation tubing to the endoscope, and one or more open egress ports on the endoscope for passive fluid drainage. Nineteen consecutive patients were identified on review (average age ± SD, 4.3 ± 4.1 years). Procedures performed ...
Potentiation of halogenated pyrimidine radiosensitizers by à -lapachone (3,4-dihydro-2,2-dimethyl-2H-naphtho[1,2-b]pyran-5,6-dione), a novel DNA repair ...
Dbait is a new drug strategy, which inhibits DNA repair machinery. Therefore, we aim to investigate its molecular mechanisms and cell cycle modifications in normal and cancer cells following Dbait and irradiation treatment, in order to understand if the radiosensitising effect of Dbait is related to a cell cycle defect of tumour ...
is normally a tyrosine kinase gene that takes on an essential part in the introduction of regular haematopoiesis. individuals with hematological neoplasms but can also be beneficial to deal with individuals with arthritis rheumatoid or additional inflammatory illnesses. 1. Intro Myeloproliferative neoplasms (MPNs) are clonal disorders until now seen as a the autonomous proliferation of dedicated hematopoietic progenitors supplementary for an Rabbit polyclonal to ACAD9 aberrant activation of tyrosine kinase (TK) signalling pathways in conjunction with an exaggerated response to hematopoietic cytokines and development elements [1, 2]. Constitutive activation of TKs can be a regular molecular personal in cell proliferation. Types of Constitutive activation of TKs are viewing in solid tumours [3, 4], arthritis rheumatoid [5], and hematopoietic malignancies [6]. Known systems of TK activation may derive from obtained heterozygote of homozygote stage mutations [7, 8], inner tandem duplications [9], ...
OBJECTIVE- Tall-like receptor (TLR)4 has been implicated in the pathogenesis of free fatty acid (FFA)-induced insulin resistance by activating inflammatory pathways including inhibitor of κB (IκB)/nuclear factor κB (NFκB). were measured in muscle biopsies in 7 lean 8 obese and 14 type 2 diabetic subjects. A primary human myotube culture system was used to Rabbit Polyclonal to RED. examine whether FFAs stimulate IκB/NFκB via TLR4 and whether FFAs increase TLR4 expression/content in muscle. RESULTS- Obese and type 2 diabetic subjects had significantly elevated TLR4 gene expression and protein content in muscle. TLR4 muscle protein content correlated with the severity of insulin resistance. Obese and type 2 diabetic subjects also had lower IκBα content an indication of elevated IκB/NFκB signaling. The increase in TLR4 and NFκB signaling was accompanied by elevated expression of the NFκB-regulated genes interleukin (IL)-6 and superoxide dismutase (SOD)2. In primary human myotubes acute ...
Background and Purpose Mind vascular endothelial cells express histamine H1 and H2 receptors, which regulate mind capillary permeability. receptor gene (in mind blood vessels by activating H4 receptors, as the H4 receptor-specific inverse agonists/antagonist JNJ 7777120, but not ciproxifan, H3 receptor antagonist, dose-dependently clogged this effect in RBE4 cells. Conclusions and Implications Both and receptors are indicated in rat mind endothelial cells, and activation of the histamine H4 buy DAPT receptor activates the Erk1/2 cascade. H3 and H4 receptors in endothelial cells are potentially important for rules of bloodCbrain barrier permeability, including trafficking of immunocompetent cells. Linked Articles This short article is definitely portion of a themed issue on Histamine Pharmacology Upgrade. To view the additional articles in this problem check out http://dx.doi.org/10.1111/bph.2013.170.issue-1 hybridization, but antibodies made against peptides derived from the H4 receptor sequence ...
178366-78-4 - 6,8-Diallyl 5,7-dihydroxy 2-(2-allyl 3-hydroxy 4-methoxyphenyl)1-H benzo(b)pyran-4-one - Searchable synonyms, formulas, resource links, and other chemical information.
multiple nucleopolyhedrovirus (AcMNPV), a known person in the sort We alphabaculoviruses, can transduce and deliver an operating gene to a variety of non-host cells, including many mammalian lines and major cells, a house mediated from the envelope fusion proteins GP64. appealing cell-targeting features. By seamlessly swapping the indigenous coding series with each of five sequences encoding different F protein, a couple of F-pseudotyped AcMNPV was produced. This report information their relative capabilities both to functionally replace GP64 in viral development also to transduce human being Saos-2 and HeLa cells. All Rabbit Polyclonal to KLRC1 five backed viable attacks in insect cell ethnicities and one, the NPV (MacoNPV) F pseudotype, could be amplified to titres close to those of native AcMNPV. In contrast, none was able to transduce the Saos-2 and HeLa cell lines. The strong support provided by MacoNPV F in computer virus production makes the corresponding pseudotype a viable scaffold to ...
The British Racing Drivers Club, owners of the Silverstone circuit, has rejected an offer that would see the British Grand Prix run at the venue until at least 2015, the FOM said today in a statement.
Thiruvananthapuram: The state-run Bekal Resorts Development Corporation (BRDC) has chalked out ambitious and novel schemes to tap the hitherto unexplored
Enzymatic basis for most drugs such as a reservoir which nifedipine was prompt discharge and thereby limiting factor in concentration of atrial pacemaker shifts to remove Avodart online cheap generic any passive immunity in the benefit has been included in the consequence of either by extensive resections can be hydrolyzed back to be far larger contractions are gradual but not in sleeping partners have undergone occasional use of Bay ...
The title meroterpene neoaustin {systematic name: (1S,2R,3S,7R,9S,11S,12R)-11-hydroxy- 2,2,2,9,12-pentamethyl-6,15-dimethylene-2,6-dihydro-13-oxaspiro[pyran-3,5-tetracyclo[7.5.1.0(1,11).0(2,7)]pentadecane ...
Dr. Tomkinson has disclosed three inventions to STC, received three UNM-affiliated issued U. S. patents, and has three pending U. S. patent applications for his DNA repair inhibitor technologies for the treatment of cancer.. There is growing interest in the identification of DNA repair inhibitors that will enhance the cytotoxicity of DNA-damaging chemotherapy drugs. Combining the two have the potential to increase the killing of cancer cells and reduce damage to normal tissues and cells if either the chemotherapy or the inhibitor could be selectively delivered to the cancer cells. Since DNA ligation (linking) is required during DNA replication and is the last step of almost all DNA repair pathways, DNA ligase inhibitors will have multiple effects, including inhibiting cell proliferation and increasing sensitivity to DNA damage.. Dr. Tomkinsons cancer technologies are small molecule inhibitors that sensitize cancer cells to DNA damage. These compounds and their derivatives are being evaluated as ...
Trivial name: Asperpyrone A. Systematic name: 4H-​Naphtho[2,​3-​b]​pyran-​4-​one, 10-​(5,​8-​dihydroxy-​2,​10-​dimethoxy-​4-​oxo-​4H-​naphtho[1,​2-​b]​pyran-​9-​yl)​-​5-​hydroxy-​6,​8-​dimethoxy-​2-​methyl-​, (+)​-. Molecular formulae: C31 H24 O11. Molecular weight: 572.52. Chemical abstract number: 491868-98-5. Literature reference:. ...
In order to study the mechanism of induction of mutations and chromosome aberrations by ionizing radiations, it is particularly useful to have available radiation-sensitive mutants. While several X-ray-sensitive rodent cell lines are available, they have been selected rather nonspecifically. It was determined that selection for resistance to the DNA replication/repair inhibitor, 1-β-d-arabinofuranosylcytosine (ara-C), would permit production of a set of X-ray-sensitive mutant cell lines that would be defective in the resynthesis step of excision or recombination repair. Such mutant cells could also be used for the isolation and characterization of human DNA repair genes. In particular, it was predicted that the repair gene defective in individuals with ataxia telangiectasia (AT) might be amenable to study with ara-C-resistant (X-ray-sensitive) mutants, since additional studies, presented here, have shown that AT cells are resistant to ara-C. In the long term, it is hoped that determining the ...
Description. This car is in fully restored and race ready order having been maintained and prepared by Sid Holle Racing & Restorations UK . Full Uk issued RAC MSA FIA papers accompany the car making it eligible for some of the most prestigious history events. Such as ( BRDC 50s Spots Car Series ,The HGPCA Drum Brake Series , The Gentleman Drivers , Goodwood Revival ,Le Mans Classic and the VSCC. For work carried out to the Bobtail between 25th and 28th March 1999 a breif descrition of wich follows:- Remove bodywork and driveschaft,check and repair damage threads.Refit schaft& checkgear selection.Remove gearbox and completely strip in order to make necessary checks,inspection and repairs etc. Supplied and fitted new 3rd Gear. Free-off top hat bush on shaft splines, supplied and fitted new gear top hat bush and free-off bearing fit on schaft. Assemble shaft and check gear positions, check throw of selector forks etc. clean off welded selector rods, trim gears in order to obtain correct end float ...
3,4-Dihydro-2,2-dimethyl-2H-naphthol[1,2-b]pyran-5,6-dione (ARQ 501; β-lapachone) showed promising anticancer activity in phase I clinical trials as monotherapy and in combination with cytotoxic drugs. ARQ 501 is currently in multiple phase II clinical trials. In vitro incubation in fresh whole blood at 37°C revealed that ARQ 501 is stable in plasma but disappears rapidly in whole blood. Our data showed that extensive metabolism in red blood cells (RBCs) was mainly responsible for the rapid disappearance of ARQ 501 in whole blood. By comparison, covalent binding of ARQ 501 and/or its metabolites to whole blood components was a minor contributor to the disappearance of this compound. Sequestration of intact ARQ 501 in RBCs was not observed. Cross-species metabolite profiles from incubating [14C]ARQ 501 in freshly drawn blood were characterized using a liquid chromatography-mass spec-trometry-accurate radioactivity counter. The results show that ARQ 501 was metabolized more rapidly in mouse and ...
Disclosed is a method for the recovery of crystalline therephthalic acid containing less than 150 ppmw p-toluic acid by subjecting a solution of therephthalic acid containing minor amounts of p-toluic acid to crystallization in a crystallization zone comprising a plurality of series-connected crystallizers wherein the solution is subjected to rate-controlled evaporative cooling by sequential reduction in pressure and temperature to cause crystallization of therephthalic acid, wherein the pressure of the solution at the end of the crystallization zone is ambient pressure or less. Solvent which is evaporated from the crystallizers is collected and condensed and the condensed solvent is returned to the crystallization zone at a point subsequent to the crystallizer from which it was obtained.
Other names: 2H-Pyran-2-one, 5,6-dihydro-6-pentyl-, (R)-; (R)-(-)-Massoilactone; Massoia lactone; 6-Pentyl-5,6-dihydro-2H-pyran-2-one, (6R)-; 5,6-Dihydro-6-pentyl-(2H)-pyran-2-one-, (R); Massoya lactone; Cocolactone; 5,6-Dihydro-6-pentyl-(2H)-pyran-2-one, massoi lactone; 2H-Pyran-2-one, 5,6-dihydro-6-pentyl-, (6R)-; 5-Pentylpent-2-en-5-olide; 6-pentyl-5,6-dihydropyran-2-one ...
Bovine respiratory disease complex (BRDC) is the most common, and costly, disease in feed yard cattle. A review of the literature shows a correlation between the diagnosis of BRDC ante-mortem and respiratory lesions at slaughter. The objectives of the studies reported here were to: 1) validate a thoracic auscultation scoring system by correlating ante-mortem lung sounds with post-mortem lung lesions and 2) evaluate thoracic auscultation and rectal temperature as diagnostic tools to predict case outcome in the feeder cattle treated for BRDC. First, a prospective cohort study involving thirty four head of cattle that had been realized from commercial cattle feeding operations were used to validate the use of a lung auscultation scoring system to identify cattle suffering from BRDC. Ante-mortem auscultation scores were compared to post-mortem lung lesions evaluated using a previously described scoring system. There was a positive correlation (P , .0001) between ante-mortem lung auscultation scores ...
CAS Name: (4aS,4bS,6aS,7S,9aS,9bR,11aS)-Tetradecahydro-7-hydroxy-4a,6a,7-trimethylcyclopenta[5,6]naphtho[1,2-c]pyran-2(1H)-one ...
The study of conversion of AF to sinus rhythm provides information on changes associated with the resumption of atrial contraction and AV synchrony leading to a regularized, rate-controlled ventricular response. Previous studies have documented improvement in cardiac output ([75]) and cardiopulmonary exercise testing variables of ventricular rate control, maximal oxygen uptake and anaerobic threshold ([76, 77]) after cardioversion to sinus rhythm. More recently, a small, prospective study of patients with a diagnosis of idiopathic dilated cardiomyopathy and chronic AF reported a significant increase in left ventricular ejection fraction after pharmacologic or electrical cardioversion to sinus rhythm ([78]). Whether improvement in cardiac function was due to restoration of atrial systolic function and AV synchrony versus reversal of an underlying cardiomyopathy associated with rapid and irregular ventricular rates during AF was not resolved by this study. Subsequent study of atrial and ...
6,8-diallyl 5,7-dihydroxy 2-(2-allyl 3-hydroxy 4-methoxyphenyl)1-H benzo(b)pyran-4-one: inhibitor of cell adhesion and atherosclerosis that targets nadph oxidase
In 2007, Bird raced in the British Formula 3 Championship with Carlin Motorsport, racing in a Mercedes powered Dallara. In March 2007, Bird secured sponsorship from BP, The brand is already prominent in the World Rally Championship Mark Reader, BPs UK Fuels Marketing Manager, commented, Sams an incredible prospect and were excited to be getting into a relationship at this stage of his career he added.[2] Bird was elected to the Motor Sports Association Race Elite Scheme in April 2007, along with 5 other drivers in various British series and also participated in a series of aerodynamic tests with the AT&T Williams F1 Team. Bird moved to the Manor Motorsport and the Formula 3 Euro Series in 2008 and had a testing year, finishing eleventh in the championship with 23 points - 16 of which came from second places during Saturday races at Catalunya and Le Mans and only picked up points from three other races. For 2009, he joined McLaren Autosport BRDC Award winner Alexander Sims, 2008 Mücke ...
ID 2: 466. Toxin: y. Trivial name: 2,​4,​6,​8,​10,​12-​Tridecahexaenoic acid, 5-​hydroxy-​3-​methoxy-​4,​12-​dimethyl-​13-​(tetrahydro-​3,​4-​dihydroxy-​2,​4,​5-​trimethyl-​2-​furyl)​-​, δ-​lactone (7CI); 2H-​Pyran-​2-​one, 4-​methoxy-​5-​methyl-​6-​[7-​methyl-​8-​(tetrahydro-​3,​4-​dihydroxy-​2,​4,​5-​trimethyl-​2-​furanyl)​-​1,​3,​5,​7-​octatetraenyl]​-​, [2S-​[2α(1E,​3E,​5E,​7E)​,​3β,​4α,​5α]​]​-; 2H-​Pyran-​2-​one, 4-​methoxy-​5-​methyl-​6-​[7-​methyl-​8-​(tetrahydro-​3,​4-​dihydroxy-​2,​4,​5-​trimethyl-​2-​furyl)​-​1,​3,​5,​7-​octatetraenyl]​- (8CI); D-​Iditol, 2,​5-​anhydro-​1,​6-​dideoxy-​2-​C-​[(1E,​3E,​5E,​7E)​-​8-​(4-​methoxy-​5-​methyl-​2-​oxo-​2H-​pyran-​6-​yl)​-​2-​methyl-​1,​3,​5,​7-​octatetraenyl]​-​4-​C-​methyl- (9CI); ...
N-(4-methylcyclohexyl)acetamide 8-[3-(4-quinolin-2-ylpiperazin-1-yl)propyl]-1,7,8-triazabicyclo[4.3.0]nona-2,4,6-trien-9-one N-(2-dimethylaminoethyl)-4-(3-methylbutoxy)benzenecarbothioamide Methyl 3-(phenylthio)isobutyrate 2-Butenedioic acid (2Z)-,polymers,polymer with 1-propene 1H-Naphtho[2,1-b]pyran-1-one,2,3,4a,8,9,10,- 10a,10b-octahydro-10-hydroxy-4a,8,10btrimethyl- manganese; 6H-pyridine; 3,4,5,6-tetrahydro-2H-pyridine; dithiocyanate methyl 2-(2-methyl-1H-indol-3-yl)-2-oxo-acetate Rosin, tall-oil, maleated, reaction products with formaldehyde, ammonium salts 4,4-dimethylhexa-2,5-dienal
A derivative of dicoumarol, C25H16O6, Mr, = 412·41, orthorhombic, P212121, a = 7·959(2), b = 12·865 (3), c = 18·606 (6) Å, V = 1905·3 (22) Å3, Z = 4, Dx = 1.44 g cm-3, λ(Mo Kα) = 0·71073 Å , μ = 0·965 cm-1, F(000) = 856, T = 293 K, final R = 0·042 for 2031 observations. The 4-hydroxycoumarins are intramolecularly hydrogen bonded between hydroxyls and carbonyls, O···O separations are 2·624 (3) and 2·718 (3)Å, a scheme which imparts a dis-symmetry to the otherwise achiral molecule and underlies packing in a polar space group.
Structure, properties, spectra, suppliers and links for: 1-[(1S,4aS,7Z,11aR)-1-Acetoxy-7-methyl-11-methylene-1,4a,5,6,9,10,11,11a-octahydrocyclonona[c]py.
The objective of my research was to generate novel information concerning the epidemiology, diagnosis and prevention of bovine respiratory disease complex (BRDC), a common pre-weaning and post-weaning beef calf disease. To reach my objective, I conducted three prospective field trials within post-weaned calf populations, and one retrospective study of pre-weaned calves utilizing survey data. I evaluated differences in behavior, health and performance in calves receiving multiple component health programs. Calves in a minimally invasive program, which included primarily non-injectable products, displayed less aversion to initial product administration but experienced higher BRDC morbidity (P = 0.02) and poorer performance (P = 0.04) compared to calves in a more invasive (all injectable products) program. Secondly, in a study of Mannheimia haemolytica inoculated calves, I found that no parameter included in physical examinations, or common blood component evaluations could discern health from ...
Bovine respiratory disease complex (BRDC) is a multi-factorial disease in which numerous factors, such as animal management, pathogen exposure and environm..
Cooley, R.B., Rhoads, T.W., Arp, D.J. and Karplus, P.A. (2011) A diiron protein autogenerates a valine-phenylalanine cross-link. Science 332, 929 ...
TY - JOUR. T1 - Γ-H2AX kinetics as a novel approach to high content screening for small molecule radiosensitizers. AU - Fu, Shibo. AU - Yang, Ying. AU - Tirtha, Das. AU - Yen, Yun. AU - Zhou, Bing sen. AU - Zhou, Ming Ming. AU - Ohlmeyer, Michael. AU - Ko, Eric C.. AU - Cagan, Ross. AU - Rosenstein, Barry S.. AU - Chen, Shu hsia. AU - Kao, Johnny. PY - 2012/6/29. Y1 - 2012/6/29. N2 - Background: Persistence of γ-H2AX after ionizing radiation (IR) or drug therapy is a robust reporter of unrepaired DNA double strand breaks in treated cells. Methods: DU-145 prostate cancer cells were treated with a chemical library ±IR and assayed for persistence of γ-H2AX using an automated 96-well immunocytochemistry assay at 4 hours after treatment. Hits that resulted in persistence of γ-H2AX foci were tested for effects on cell survival. The molecular targets of hits were validated by molecular, genetic and biochemical assays and in vivo activity was tested in a validated Drosophila cancer model. Results: ...
We have used recombinant clones derived from microdissection of the fragile X region to characterize breakpoints around the fragile site at Xq27.3. So far, no microdissection markers derived from Xq28 material have been found, thus allowing a rapid screening for clones surrounding the fragile site by their presence in a somatic cell hybrid containing Xq27.2-Xqter. A total of 43 new DNA markers from Xq27 have been sublocalized within this chromosome band. Of these new DNA markers, 5 lie in an interval defined as containing the fragile X region. The saturation of Xq27 with DNA markers by microdissection demonstrates the power of this technique and provides the resources for generating a complete physical map of the region.
Get quotes fast and Choose from 1 clinic(s) offering Corneal CrossLink (CXL) treatment in Coahiula Get quotes fast & choose the best with phone numbers, reviews, prices, maps and pictures. | Page 0
... bromodeoxycytidine MeSH D13.570.230.329.950 - zalcitabine MeSH D13.570.230.329.950.500 - lamivudine MeSH D13.570.230.360 - ... bromodeoxycytidine MeSH D13.570.685.245.500.950 - zalcitabine MeSH D13.570.685.245.500.950.500 - lamivudine MeSH D13.570. ...
1962) The distribution and fate of bromodeoxyuridine and bromodeoxycytidine in the mouse and rat. Cancer Res 22:254-265. ...
Kriss JP, Revesz L. Distribution and fate of bromodeoxyuridine and bromodeoxycytidine in mouse and rat. Cancer Res. 1962; 22: ...
Kriss JP, Revesz L. The distribution and fate of bromodeoxyuridine and bromodeoxycytidine in the mouse and rat. Cancer Res. ...
The effect of inhibition of cytidine deaminase by tetrahydrouridineon the utilization of deoxycytidineand 5-bromodeoxycytidine ...
Arteriogenesis (smooth muscle actin+, artery number, and diameter) and remodeling [vimentin+, 5-bromodeoxycytidine (BrdU)+, ...
Cells were cultured in fresh medium supplemented with 20 μM bromodeoxyuridine (BrdU)/bromodeoxycytidine (BrdC) (3:1) for 16 to ...
Radiosensitization of rat glioma with bromodeoxycytidine and adenovirus expressing herpes simplex virus-thymidine kinase ...
... bromodeoxycytidine MeSH D13.570.230.329.950 - zalcitabine MeSH D13.570.230.329.950.500 - lamivudine MeSH D13.570.230.360 - ... bromodeoxycytidine MeSH D13.570.685.245.500.950 - zalcitabine MeSH D13.570.685.245.500.950.500 - lamivudine MeSH D13.570. ...
... and bromodeoxycytidine (BC) and are then collected in mitosis. Because DNA synthesis is semiconservative, opposite strands of ...
For example, following 5-bromodeoxycytidine sensitization an X-ray DER value of 1.87 ± 0.04 at 1% survival was increased to ... For example, following 5-bromodeoxycytidine sensitization an X-ray DER value of 1.87 ± 0.04 at 1% survival was increased to ... For example, following 5-bromodeoxycytidine sensitization an X-ray DER value of 1.87 ± 0.04 at 1% survival was increased to ... For example, following 5-bromodeoxycytidine sensitization an X-ray DER value of 1.87 ± 0.04 at 1% survival was increased to ...
Favus, M. J., Schneider, A. B., Stachura, M. E., Arnold, J. E., Ryo, U. Y., Pinsky, S. M., Colman, M., Arnold, M. J. & Frohman, L. A., May 6 1976, In: New England Journal of Medicine. 294, 19, p. 1019-1025 7 p.. Research output: Contribution to journal › Article › peer-review ...
... using 5-bromodeoxycytidine as the selective agent. A hybrid line between this double mutant and a human diploid fibroblast was ... using 5-bromodeoxycytidine as the selective agent. A hybrid line between this double mutant and a human diploid fibroblast was ... using 5-bromodeoxycytidine as the selective agent. A hybrid line between this double mutant and a human diploid fibroblast was ... using 5-bromodeoxycytidine as the selective agent. A hybrid line between this double mutant and a human diploid fibroblast was ...
bromodeoxycytidine in the mouse and rat. Cancer Res 1962, 22:254-265. 12. Matiašová A, Ševc J, Mikeš J, Jendželovský R, ...
Effects of 5-bromodeoxycytidine in Escherichia coli and bacteriophage. To address its role in myocardial metabolism, we ...
... bromodeoxycytidine), hormones (adrenocorticosteroids, prednisone, dexamethasone, aminoglutethimide), progestins ( ...
revised radiosensitization of rat RT2 psychedelics with bromo- deoxycytidine and small Gwneric using the Purchase v-tada super ...
... bromodeoxycytidine, fluorodeoxyuridine (FudR), hydroxyurea, cisplatin, and therapeutically effective analogs and derivatives of ...
... bromodeoxycytidine, fluorodeoxyuridine (FudR), hydroxyurea, cisplatin, and therapeutically effective analogs and derivatives of ...
A dideoxynucleoside compound in which the 3-hydroxy group on the sugar moiety has been replaced by a hydrogen. This modification prevents the formation of phosphodiester linkages which are needed for the completion of nucleic acid chains. The compound is a potent inhibitor of HIV replication at low concentrations, acting as a chain-terminator of viral DNA by binding to reverse transcriptase. Its principal toxic side effect is axonal degeneration resulting in peripheral neuropathy ...
MeSH D13.570.230.329.100 --- bromodeoxycytidine * MeSH D13.570.230.329.950 --- zalcitabine * MeSH D13.570.230.329.950.500 --- ...
Humans , Animals , Sister Chromatid Exchange , Bromodeoxycytidine , Bromodeoxyuridine/pharmacology , Fluorescent Antibody ...
Humans , Animals , Sister Chromatid Exchange , Bromodeoxycytidine , Bromodeoxyuridine/pharmacology , Fluorescent Antibody ...
BrdU is commonly used in the detection of proliferating cells in living tissues.[1] 5-Bromodeoxycytidine is deaminated to form ...

No FAQ available that match "bromodeoxycytidine"