An indicator and reagent. It has been used for several purposes including the determination of serum albumin concentrations
An indicator and reagent. It has been used in serum albumin determinations and as a pH indicator.
Cresols, also known as hydroxytoluene, are a group of phenolic compounds including ortho-cresol, meta-cresol, and para-cresol, which differ in the position of the hydroxyl group on the benzene ring.
A major protein in the BLOOD. It is important in maintaining the colloidal osmotic pressure and transporting large organic molecules.
Method of analyzing chemicals using automation.
An enzyme that catalyzes the conversion of L-alanine and 2-oxoglutarate to pyruvate and L-glutamate. (From Enzyme Nomenclature, 1992) EC 2.6.1.2.
Enzymes of the transferase class that catalyze the conversion of L-aspartate and 2-ketoglutarate to oxaloacetate and L-glutamate. EC 2.6.1.1.
A non-essential amino acid that occurs in high levels in its free state in plasma. It is produced from pyruvate by transamination. It is involved in sugar and acid metabolism, increases IMMUNITY, and provides energy for muscle tissue, BRAIN, and the CENTRAL NERVOUS SYSTEM.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
A subclass of enzymes of the transferase class that catalyze the transfer of an amino group from a donor (generally an amino acid) to an acceptor (generally a 2-keto acid). Most of these enzymes are pyridoxyl phosphate proteins. (Dorland, 28th ed) EC 2.6.1.
A family of compounds containing an oxo group with the general structure of 1,5-pentanedioic acid. (From Lehninger, Principles of Biochemistry, 1982, p442)
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Albumin as an outcome measure in haemodialysis in patients: the effect of variation in assay method. (1/23)

BACKGROUND: Serum/plasma albumin is an important predictor of future mortality/morbidity in haemodialysis (HD) patients and has been proposed as an important audit measure. Different methods of albumin assay give different results and the bias between methods may be greater in renal failure patients. METHODS: Albumin concentration in plasma was measured by three methods, two dye-binding methods (bromocresol green (BCG) and bromocresol purple (BCP)) and an immuno-turbidimetric (ITM) method, in 143 HD patients (group I) and 49 non-renal patients (group II). Comparisons were made between means, variation in differences across a range of albumin concentrations and on the percentage of patients within the normal range. RESULTS: In HD patients (group I), BCG over-estimated plasma albumin compared with the other two methods. The difference could be as much as 10 g/l and was more marked in hypoalbuminaemic patients. The BCP method gave results closer to the ITM method, particularly in HD patients. These differences were less marked in group II patients but both methods overestimated albumin compared with the ITM method. Using the BCG local laboratory normal range, 84% of HD patients had plasma albumin concentrations within the normal range but this fell to 57% if the BCP results were used. CONCLUSIONS: The method for determining albumin concentration has a marked effect on the results particularly in HD patients. BCG, the most commonly used method, gives higher results than other methods and correlates poorly with an immunological method. These differences make comparative audit between nephrology units difficult and have implications for other biochemical variables and other specialties.  (+info)

Conversion between bromcresol green- and bromcresol purple-measured albumin in renal disease. (2/23)

BACKGROUND: Albumin measured by a bromcresol purple dye-binding assay (Alb(BCP)) agrees more closely with the gold standard of immunonephelometry than does bromcresol green (Alb(BCG)) measurement. Both tests are in current clinical use. A method for converting between the two would be useful. METHODS: We measured albumin by bromcresol green and bromcresol purple in 535 patients, 155 of whom had renal disease. We randomly divided data from the patients with renal disease into two equal-sized sets, and used one set to derive, and the remaining set to validate, a regression equation relating the two values. RESULTS: The relationship Alb(BCG)=5.5+Alb(BCP) performed very well in both the renal patient validation set and in the data from 380 unselected in-patients and out-patients. Intraclass correlations for agreement between measured Alb(BCG) and predicted Alb(BCG) was 0.98 in both analyses. CONCLUSIONS: The ability to convert between these measurements will be of use in clinical situations where the absolute value of the serum albumin is important, when data from laboratories using different methodologies must be combined, and in the application of the Modification of Diet in Renal Disease formula to estimate glomerular filtration rate in patients whose albumin has been measured by bromcresol purple.  (+info)

Guidance for selecting the measurement conditions in the dye-binding method for determining serum protein: theoretical analysis based on the chemical equilibrium of protein error. (3/23)

A methodology for selecting the measurement conditions in the dye-binding method for determining serum protein has been studied by a theoretical calculation. This calculation was based on the fact that a protein error occurs because of a reaction between the side chains of a positively charged amino acid residue in a protein molecule and a dissociated dye anion. The calculated characteristics of this method are summarized as follows: (1) Although the reaction between the dye and the protein occurs up to about pH 12, a change in the color shade, called protein error, is observed only in a pH region restricted within narrow limits. (2) Although the apparent absorbance (the absorbance of the test solution measured against a reagent blank) is lower than the true absorbance indicated by the formed dye-protein complex, the apparent absorbance correlates with the true absorbance with a correlation coefficient of 1.0. (3) At a higher dye concentration, the calibration curve is more linear at a higher pH than at a lower pH. Most of these characteristics were similarly observed experimentally in the reactions of BPB, BCG and BCP with human and bovine albumins. It is concluded that in order to ensure the linearity of the calibration curve, the measurement should be performed at a higher dye concentration and sufficiently high pH where the detection sensitivity is satisfied.  (+info)

Correlation of the suicide phenomenon in Aeromonas species with virulence and enteropathogenicity. (4/23)

Certain strains of mesophilic Aeromonads like A. hydrophila, A. veronii biotype sobria and A. caviae when grown in broth containing 0.5% glucose, undergo growth inhibition concomitant with acetate accumulation. As these strains become nonviable after 24 h, this phenomenon is termed suicide. We investigated suicidal strains of Aeromonas species as means of understanding animal virulence and enteropathogenicity. Non suicidal strains of A. Hydrophila showed and overall 88.8% lethality rate and non suicidal strains of A. veronii biotype sobria showed 83.3% lethality rate and was nil for its suicidal part. Of the two suicidal A. caviae strains tested, none were lethal. The present data suggest that the suicide phenomenon may explain strain specific [A. veronii biotype sobria, A. hydrophila] and species specific [A. caviae] virulence and enteropathogenicity.  (+info)

Measurement of serum albumin by capillary zone electrophoresis, bromocresol green, bromocresol purple, and immunoassay methods. (5/23)

BACKGROUND/AIMS: The introduction of capillary zone electrophoresis (CZE) to this laboratory has highlighted discrepancies in albumin measured on an Abbott Aeroset by bromocresol green (BCG) and that calculated by CZE on the basis of total protein measured by Biuret. METHODS: This study examined differences in albumin estimation by CZE, Abbott Aeroset BCG, and Aeroset bromocresol purple (BCP), and compared these with albumin estimated by Beckman Array immunoassay. RESULTS: Altman and Bland analysis of results showed a positive bias of BCG with CZE (4.51 g/litre; 95% limits of agreement, 3.77 to 5.26; n = 72) and BCP (3.85 g/litre; 95% limits of agreement, -1.42 to 9.12; n = 72). CZE and BCP agreed closely (0.67 g/litre; 95% limits of agreement, -4.39 to 3.06; n = 72). Analysis of 57 of those samples in which BCG and CZE differed > or = 5 g/litre showed a positive bias of BCG with immunoassay (8.35 g/litre; 95% limits of agreement, 1.54 to 15.16; n =57), with good agreement between CZE and immunoassay (-0.44 g/litre; 95% limits of agreement, -2.82 to 1.94; n = 57). CONCLUSIONS: BCP is superior to BCG for the assay of albumin and has replaced BCG as the routine test for albumin in this laboratory.  (+info)

Identification of the human mitochondrial S-adenosylmethionine transporter: bacterial expression, reconstitution, functional characterization and tissue distribution. (6/23)

The mitochondrial carriers are a family of transport proteins that, with a few exceptions, are found in the inner membranes of mitochondria. They shuttle metabolites and cofactors through this membrane, and connect cytoplasmic functions with others in the matrix. SAM (S-adenosylmethionine) has to be transported into the mitochondria where it is converted into S-adenosylhomocysteine in methylation reactions of DNA, RNA and proteins. The transport of SAM has been investigated in rat liver mitochondria, but no protein has ever been associated with this activity. By using information derived from the phylogenetically distant yeast mitochondrial carrier for SAM and from related human expressed sequence tags, a human cDNA sequence was completed. This sequence was overexpressed in bacteria, and its product was purified, reconstituted into phospholipid vesicles and identified from its transport properties as the human mitochondrial SAM carrier (SAMC). Unlike the yeast orthologue, SAMC catalysed virtually only countertransport, exhibited a higher transport affinity for SAM and was strongly inhibited by tannic acid and Bromocresol Purple. SAMC was found to be expressed in all human tissues examined and was localized to the mitochondria. The physiological role of SAMC is probably to exchange cytosolic SAM for mitochondrial S-adenosylhomocysteine. This is the first report describing the identification and characterization of the human SAMC and its gene.  (+info)

Theoretical analysis concerning the characteristics of a dye-binding method for determining serum protein based on protein error of pH indicator: effect of buffer concentration of the color reagent on the color development. (7/23)

In the dye-binding method based on protein error of a pH indicator, the color development has been reported to be markedly affected by the buffer concentration of the color reagent. In this study, the author analyzed this phenomenon by a theoretical calculation based on the chemical equilibrium of protein error. The calculation was performed on the assumption that both the dissociated dye anion and the anion contained in the buffer solution react with protein, forming a dye-protein complex and an anion-protein complex, respectively. The calculated results were compared with those obtained by the experiments using bromophenol blue, bromocresol green and bromocresol purple that are employed widely for determining the human serum albumin concentration clinically. The calculated results of this method are summarized as follows: (1) the color development decreases with the increase in the concentration of the anion contained in the buffer solution; (2) the calibration curve is more linear in the higher concentration of the anion than in the lower one. These calculated results agreed well with the experimental ones. From these results, it was concluded that the change in the color development by the buffer concentration of the color reagent is due to the change in the concentration of the buffer anion.  (+info)

Measuring albumin and calcium in serum in a dual test with the Hitachi 704. (8/23)

We describe a method for simultaneously determining albumin, by using bromcresol purple, and calcium, by using Arsenazo III, in the same analytical cuvette on the Hitachi 704. Both assays agree well with accepted procedures. The standard curves for the albumin and calcium assays are linear from 0 to 60 g/L and 0 to 5.0 mmol/L, respectively. Calibration is stable for 7 days with use of open reagent in the instrument. Both assays are unaffected by hemoglobin less than or equal to 5 g/L and Intralipid less than or equal to 4 g/L; calcium is unaffected by bilirubin less than or equal to 600 mumol/L.  (+info)

Bromcresol purple is a pH indicator dye that is commonly used in medical and laboratory settings to determine the acidity or alkalinity (pH level) of various substances. It is often used in clinical chemistry to monitor the pH of blood, urine, and other bodily fluids.

When added to a solution, bromcresol purple changes color depending on the pH of the solution. At a pH below 5.2, it appears yellow, while at a pH above 6.8, it turns purple. In between these values, it takes on various shades of greenish-blue, with the exact shade corresponding to the pH level of the solution.

Bromcresol purple is also used in some types of litmus paper and in certain medical tests, such as the bromcresol purple test for proteinuria (the presence of excess protein in the urine), where it can help detect changes in the pH of urine that may indicate kidney disease or other health problems.

Bromcresol green is a pH indicator dye that is commonly used in medical and laboratory settings to determine the acidity or alkalinity (pH level) of various substances. It has a green color in its unionized form, which appears at a pH range of 3.8 to 5.4. When the pH rises above 5.4, bromcresol green turns blue, indicating an alkaline environment.

In medical testing, bromcresol green is often used in urinalysis and other bodily fluid analysis to assess acid-base balance. It can also be used as a component of certain culture media for microbiological tests. However, it's worth noting that bromcresol green has been largely replaced by other pH indicators like phenolphthalein and methyl orange in many applications due to its lower sensitivity and specificity.

Cresols are a group of chemical compounds that are phenolic derivatives of benzene, consisting of methyl substituted cresidines. They have the formula C6H4(OH)(\_3CH3). There are three isomers of cresol, depending on the position of the methyl group: ortho-cresol (m-cresol), meta-cresol (p-cresol), and para-cresol (o-cresol). Cresols are used as disinfectants, antiseptics, and preservatives in various industrial and commercial applications. They have a characteristic odor and are soluble in alcohol and ether. In medical terms, cresols may be used as topical antiseptic agents, but they can also cause skin irritation and sensitization.

Serum albumin is the most abundant protein in human blood plasma, synthesized by the liver. It plays a crucial role in maintaining the oncotic pressure or colloid osmotic pressure of blood, which helps to regulate the fluid balance between the intravascular and extravascular spaces.

Serum albumin has a molecular weight of around 66 kDa and is composed of a single polypeptide chain. It contains several binding sites for various endogenous and exogenous substances, such as bilirubin, fatty acids, hormones, and drugs, facilitating their transport throughout the body. Additionally, albumin possesses antioxidant properties, protecting against oxidative damage.

Albumin levels in the blood are often used as a clinical indicator of liver function, nutritional status, and overall health. Low serum albumin levels may suggest liver disease, malnutrition, inflammation, or kidney dysfunction.

"Autoanalysis" is not a term that is widely used in the medical field. However, in psychology and psychotherapy, "autoanalysis" refers to the process of self-analysis or self-examination, where an individual analyzes their own thoughts, feelings, behaviors, and experiences to gain insight into their unconscious mind and understand their motivations, conflicts, and emotional patterns.

Self-analysis can involve various techniques such as introspection, journaling, meditation, dream analysis, and reflection on past experiences. While autoanalysis can be a useful tool for personal growth and self-awareness, it is generally considered less reliable and comprehensive than professional psychotherapy or psychoanalysis, which involves a trained therapist or analyst who can provide objective feedback, interpretation, and guidance.

Alanine transaminase (ALT) is a type of enzyme found primarily in the cells of the liver and, to a lesser extent, in the cells of other tissues such as the heart, muscles, and kidneys. Its primary function is to catalyze the reversible transfer of an amino group from alanine to another alpha-keto acid, usually pyruvate, to form pyruvate and another amino acid, usually glutamate. This process is known as the transamination reaction.

When liver cells are damaged or destroyed due to various reasons such as hepatitis, alcohol abuse, nonalcoholic fatty liver disease, or drug-induced liver injury, ALT is released into the bloodstream. Therefore, measuring the level of ALT in the blood is a useful diagnostic tool for evaluating liver function and detecting liver damage. Normal ALT levels vary depending on the laboratory, but typically range from 7 to 56 units per liter (U/L) for men and 6 to 45 U/L for women. Elevated ALT levels may indicate liver injury or disease, although other factors such as muscle damage or heart disease can also cause elevations in ALT.

Aspartate aminotransferases (ASTs) are a group of enzymes found in various tissues throughout the body, including the heart, liver, and muscles. They play a crucial role in the metabolic process of transferring amino groups between different molecules.

In medical terms, AST is often used as a blood test to measure the level of this enzyme in the serum. Elevated levels of AST can indicate damage or injury to tissues that contain this enzyme, such as the liver or heart. For example, liver disease, including hepatitis and cirrhosis, can cause elevated AST levels due to damage to liver cells. Similarly, heart attacks can also result in increased AST levels due to damage to heart muscle tissue.

It is important to note that an AST test alone cannot diagnose a specific medical condition, but it can provide valuable information when used in conjunction with other diagnostic tests and clinical evaluation.

Alanine is an alpha-amino acid that is used in the biosynthesis of proteins. The molecular formula for alanine is C3H7NO2. It is a non-essential amino acid, which means that it can be produced by the human body through the conversion of other nutrients, such as pyruvate, and does not need to be obtained directly from the diet.

Alanine is classified as an aliphatic amino acid because it contains a simple carbon side chain. It is also a non-polar amino acid, which means that it is hydrophobic and tends to repel water. Alanine plays a role in the metabolism of glucose and helps to regulate blood sugar levels. It is also involved in the transfer of nitrogen between tissues and helps to maintain the balance of nitrogen in the body.

In addition to its role as a building block of proteins, alanine is also used as a neurotransmitter in the brain and has been shown to have a calming effect on the nervous system. It is found in many foods, including meats, poultry, fish, eggs, dairy products, and legumes.

Sensitivity and specificity are statistical measures used to describe the performance of a diagnostic test or screening tool in identifying true positive and true negative results.

* Sensitivity refers to the proportion of people who have a particular condition (true positives) who are correctly identified by the test. It is also known as the "true positive rate" or "recall." A highly sensitive test will identify most or all of the people with the condition, but may also produce more false positives.
* Specificity refers to the proportion of people who do not have a particular condition (true negatives) who are correctly identified by the test. It is also known as the "true negative rate." A highly specific test will identify most or all of the people without the condition, but may also produce more false negatives.

In medical testing, both sensitivity and specificity are important considerations when evaluating a diagnostic test. High sensitivity is desirable for screening tests that aim to identify as many cases of a condition as possible, while high specificity is desirable for confirmatory tests that aim to rule out the condition in people who do not have it.

It's worth noting that sensitivity and specificity are often influenced by factors such as the prevalence of the condition in the population being tested, the threshold used to define a positive result, and the reliability and validity of the test itself. Therefore, it's important to consider these factors when interpreting the results of a diagnostic test.

Transaminases, also known as aminotransferases, are a group of enzymes found in various tissues of the body, particularly in the liver, heart, muscle, and kidneys. They play a crucial role in the metabolism of amino acids, the building blocks of proteins.

There are two major types of transaminases: aspartate aminotransferase (AST) and alanine aminotransferase (ALT). Both enzymes are normally present in low concentrations in the bloodstream. However, when tissues that contain these enzymes are damaged or injured, such as during liver disease or muscle damage, the levels of AST and ALT in the blood may significantly increase.

Measurement of serum transaminase levels is a common laboratory test used to assess liver function and detect liver injury or damage. Increased levels of these enzymes in the blood can indicate conditions such as hepatitis, liver cirrhosis, drug-induced liver injury, heart attack, and muscle disorders. It's important to note that while elevated transaminase levels may suggest liver disease, they do not specify the type or cause of the condition, and further diagnostic tests are often required for accurate diagnosis and treatment.

Alpha-ketoglutaric acid, also known as 2-oxoglutarate, is not an acid in the traditional sense but is instead a key molecule in the Krebs cycle (citric acid cycle), which is a central metabolic pathway involved in cellular respiration. Alpha-ketoglutaric acid is a crucial intermediate in the process of converting carbohydrates, fats, and proteins into energy through oxidation. It plays a vital role in amino acid synthesis and the breakdown of certain amino acids. Additionally, it serves as an essential cofactor for various enzymes involved in numerous biochemical reactions within the body. Any medical conditions or disorders related to alpha-ketoglutaric acid would typically be linked to metabolic dysfunctions or genetic defects affecting the Krebs cycle.

The liver is a large, solid organ located in the upper right portion of the abdomen, beneath the diaphragm and above the stomach. It plays a vital role in several bodily functions, including:

1. Metabolism: The liver helps to metabolize carbohydrates, fats, and proteins from the food we eat into energy and nutrients that our bodies can use.
2. Detoxification: The liver detoxifies harmful substances in the body by breaking them down into less toxic forms or excreting them through bile.
3. Synthesis: The liver synthesizes important proteins, such as albumin and clotting factors, that are necessary for proper bodily function.
4. Storage: The liver stores glucose, vitamins, and minerals that can be released when the body needs them.
5. Bile production: The liver produces bile, a digestive juice that helps to break down fats in the small intestine.
6. Immune function: The liver plays a role in the immune system by filtering out bacteria and other harmful substances from the blood.

Overall, the liver is an essential organ that plays a critical role in maintaining overall health and well-being.

20 mg of the indicator bromcresol purple is added. Different types of bacteria can be differentiated on the agar by their color ... The results are scored as follows: purple slant/yellow butt = LSI Negative turbid, purple butt = LSI Positive black precipitate ... Bacteria able to decarboxylate lysine will leave the media purple colored. Bacteria producing hydrogen sulfide will appear ...
A portable fiber-optic pesticide biosensor based on immobilized cholinesterase and sol-gel entrapped bromcresol purple for in- ...
... bromcresol green MeSH D02.455.426.559.389.832.150 - bromcresol purple MeSH D02.455.426.559.389.832.180 - bromphenol blue MeSH ... bromcresol green MeSH D02.886.590.887.150 - bromcresol purple MeSH D02.886.590.887.180 - bromphenol blue MeSH D02.886.590.887. ... bromcresol green MeSH D02.755.239.195 - bromcresol purple MeSH D02.755.239.216 - butylated hydroxytoluene MeSH D02.755.239.327 ...
bromocresol purple, 0.02 g/L meat extract, 1 g/L peptone from meat, 10 g/L sodium chloride, 5 g/L ... bromo creol purple, 0.02 g/L , dextrose, 1 g/L , L-lysine hydrochloride, 5 g/L , peptic digest of animal tissue, 5 g/L , yeast ... bromocresol purple, 4 mg/L , meat extract, 3.0 g/L , peptone, 10.0 g/L , sodium chloride, 5.0 g/L , sucrose, 10.0 g/L ... bromocresol purple, 0.02 g/L , meat extract, 1 g/L , peptone from meat, 10 g/L , sodium chloride, 5 g/L ...
20 mg of the indicator bromcresol purple is added. Different types of bacteria can be differentiated on the agar by their color ... The results are scored as follows: purple slant/yellow butt = LSI Negative turbid, purple butt = LSI Positive black precipitate ... Bacteria able to decarboxylate lysine will leave the media purple colored. Bacteria producing hydrogen sulfide will appear ...
Bromcresol purple. 5.2-6.8. 1 drop 0.1% aq. soln.. yellow. purple. Chlorphenol red. 5.4-6.8. 1 drop 0.1% aq. soln.. yellow. red ...
The method to measure albumin concentration utilizes the dye bromcresol purple (BCP). When the dye binds selectively with ... Divalent copper reacts in alkaline solution with protein peptide bonds to form the characteristic purple-colored biuret complex ...
Serum albumin (CV , 2.0%) was estimated using the bromcresol purple method. Lipids [total cholesterol (CV , 3.0%), HDL (CV , ...
The improved method utilizes bromcresol purple that forms a colored complex specifically with albumin. The intensity of the ... Pooled plasma samples (EDTA) show high albumin concentrations with the bromcresol purple assay? What could be interfering with ... DIAG-250 (QuantiChrom BCG Albumin Assay Kit) uses bromcresol green, a dye that is more (about 2-fold) sensitive than bromcresol ... purple used in the BCP albumin assay. BCG is not absolutely specific for albumin, however, it also reacts with α1- and α2- ...
... colorimetyl bromcresol purple) were measured by an autoanalyzer (Beckman Coluter Synchron system, Lx-20, Fullerton, CA, USA). ...
Bromcresol purple. 0.02 g. Distilled water. 1 liter. Dispense 4 ml portions into 13 × 100 mm tubes. Autoclave 15 min at 121°C. ...
Chinas leading Bromocresol Purple BioReagent product, with strict quality control Indicator Bromocresol Purple BioReagent ... High quality CAS 115-40-2 Bromocresol Purple BioReagent Suitable For Indicator, Dye Content 90% from China, ... factories, producing high quality 90% Bromocresol Purple BioReagent products. ... Bromcresol Purple (115-40-2). Hazard Codes. Xi,Xn. Risk Statements. 36/37/38-10-20/21/22. ...
Ketik: Order / / Indikator BromCresol Purple@500ml / Nama anda / Alamat Pengiriman Lihat Detail ...
The medium contains glucose and the pH indicating dye bromcresol purple. In late, Think Music had purchased the audio rights ...
Acidometric analyte detection was performed with bromcresol purple as pH indicator and spectrophotometry. The TMV-assisted ...
Conversion between bromcresol green‐ and bromcresol purple‐measured albumin in renal disease Journal Articles ...
A portable fiber-optic pesticide biosensor based on immobilized cholinesterase and sol-gel entrapped bromcresol purple for in- ...
Reduced pH causes a colour change in bromcresol purple indicator from purple to yellow. ... Dextrose, a fermentable carbohydrate, and bromcresol purple, a colour indicator, are both components of the Quadri formula. ... The pH indicator bromocresol purple changes colour from purple to yellow when exposed to an acidic environment. ... The medias pH changes, transforming the colour from purple to yellow.. Objective of Salt Tolerance Test. *This test is used to ...
D2.455.426.559.389.657.239.180 Bromcresol Green D2.755.239.190 D2.455.426.559.389.657.239.190 Bromcresol Purple D2.755.239.195 ...
D2.455.426.559.389.657.239.180 Bromcresol Green D2.755.239.190 D2.455.426.559.389.657.239.190 Bromcresol Purple D2.755.239.195 ...
Bromcresol purple. 0.015 g. Agar. 15.0 g. Distilled water (deionized). 1 liter. ...
3.5 g/dL by bromcresol green or 3.2 g/ dL by bromcresol purple laboratory methods (Table 4). ...
If the bacteria acidify the carbohydrate, the pH will change and the indicator (brom cresol purple) will turn yellow. ... Heart Infusion broth with 1% carbohydrate and 0.16 brom cresol purple indicator. Most of the carbohydrate broths are ... To make the test easier to read we add 0.5% dextrose and brom cresol purple indicator. ... A deep purple to black or bluish color of the agar indicates that starch has not been hydrolyzed and thus a negative test. For ...
Bromcresol Purple. BCP. Brophy College Preparatory BCP. Brown Chitin Protector BCP. Budget Change Proposal. ...
BrCP, bromcresol purple), and 0.1 m. m. HgCl2. The data expressed as the percentage of inhibition are means ± S.D. of at least ... bromcresol purple, hemicholinium-3, quinine, carboxyatractyloside, and bongkrekic acid were significant (p , 0.01, one-way ...
... indigo carmine or bromcresol purple. Further additional data showed that the chromogenic albumin indicator BCG in combination ... whereas the chromogenic albumin indicator bromcresol green (BCG) was released continuously. This had been conclusively shown in ...
D2.455.426.559.389.657.239.180 Bromcresol Green D2.755.239.190 D2.455.426.559.389.657.239.190 Bromcresol Purple D2.755.239.195 ...
Bromcresol Purple Preferred Term Term UI T005578. Date01/01/1999. LexicalTag NON. ThesaurusID ... Bromcresol Purple Preferred Concept UI. M0002932. Registry Number. 201C22C3EC. Related Numbers. 115-40-2. Scope Note. An ... Bromcresol Purple. Tree Number(s). D02.455.426.559.389.657.239.195. D02.455.426.559.389.832.150. D02.886.590.887.150. Unique ID ... Bromocresol Purple Term UI T005579. Date10/09/1986. LexicalTag NON. ThesaurusID ...
Bromcresol Purple Preferred Term Term UI T005578. Date01/01/1999. LexicalTag NON. ThesaurusID ... Bromcresol Purple Preferred Concept UI. M0002932. Registry Number. 201C22C3EC. Related Numbers. 115-40-2. Scope Note. An ... Bromcresol Purple. Tree Number(s). D02.455.426.559.389.657.239.195. D02.455.426.559.389.832.150. D02.886.590.887.150. Unique ID ... Bromocresol Purple Term UI T005579. Date10/09/1986. LexicalTag NON. ThesaurusID ...
Growth characteristics on Lowenstein-Jensen and bromcresol purple slants.. ‡Growth in presence of TCH.. §All 8 strains showed ...
Bromocresol Purple. Purple, Bromcresol. Purple, Bromocresol. Tree number(s):. D02.455.426.559.389.657.239.195. D02.455.426.559. ... Bromcresol Purple - Preferred Concept UI. M0002932. Scope note. An indicator and reagent. It has been used for several purposes ...
N0000166612 Brocresine N0000166197 Bromates N0000166922 Bromazepam N0000166615 Bromcresol Green N0000166613 Bromcresol Purple ...
Bromcresol purple binds with HSA forming colored complex with absorbance at 600 nm. Intensity of absorbance is proportional to ... The copper ions in alkaline biuret reagent react with peptide bonds of serum proteins to form a purple color. The intensity of ... paper strips are coated by reagents that become purple by action of nitrite. Many types of bacteria are producing nitrite and ... fructosamine (glycated serum protein) reduces nitroblue tetrazolium under appropriate conditions forming a purple colored ...
9 bromcresol green (BCG), and 12 bromcresol purple (BCP) methods. RESULTS: Two of 3 immunochemical procedures, 5 of 9 BCG, and ...
INDICATORS AND REAGENTS BROMCRESOL GREEN INDICATORS AND REAGENTS BROMCRESOL PURPLE INDICATORS AND REAGENTS BROMPHENOL BLUE ...
of bromcresol purple desoxycholate broth and incubated overnight. With a capillary pipette each of three tubes containing a ...
D2.455.426.559.389.657.239.180 Bromcresol Green D2.755.239.190 D2.455.426.559.389.657.239.190 Bromcresol Purple D2.755.239.195 ...
Quantitative measurement of mass concentration of albumin in serum or plasma specimen using bromcresol green technique (. ... Mass concentration of albumin in plasma by bromocresol purple dye binding method (. 1106251000000101. ) ... Mass concentration of albumin in serum by bromocresol purple dye binding method (. 1106361000000107. ) ...
... bromcresol green BCG,bromocresol green BDGF-0,bone-derived growth factor-0 BHT,beta-hydroxytheophylline BHT,borderline ... purple membrane PM,pyridoxamine PMN,peripheral blood granulocyte PMN,poly-morphonuclear PMN,poly-morphonuclear leucocyte PMN, ...
... bromcresol green BCG,bromocresol green BDGF-0,bone-derived growth factor-0 BHT,beta-hydroxytheophylline BHT,borderline ... purple membrane PM,pyridoxamine PMN,peripheral blood granulocyte PMN,poly-morphonuclear PMN,poly-morphonuclear leucocyte PMN, ...
  • The improved method utilizes bromcresol purple that forms a colored complex specifically with albumin. (bioassaysys.com)
  • DIAG-250 (QuantiChrom BCG Albumin Assay Kit) uses bromcresol green, a dye that is more (about 2-fold) sensitive than bromcresol purple used in the BCP albumin assay. (bioassaysys.com)
  • Pooled plasma samples (EDTA) show high albumin concentrations with the bromcresol purple assay? (bioassaysys.com)
  • Dextrose, a fermentable carbohydrate , and bromcresol purple, a colour indicator, are both components of the Quadri formula. (microbiologynote.com)
  • Additionally, 20 mg of the indicator bromcresol purple is added. (wikipedia.org)
  • Acidometric analyte detection was performed with bromcresol purple as pH indicator and spectrophotometry. (fh-aachen.de)
  • For this reason, a broth called brain-heart infusion (BHI) is employed, and it is augmented with 6.5% sodium chloride and bromocresol purple, which acts as a pH indicator. (microbiologynote.com)
  • Reduced pH causes a colour change in bromcresol purple indicator from purple to yellow. (microbiologynote.com)
  • The pH indicator bromocresol purple changes colour from purple to yellow when exposed to an acidic environment. (microbiologynote.com)
  • Bromocresol Purple has been used for staining of Saccharomyces cerevisiae. (yunbangpharm.com)
  • The results are scored as follows: purple slant/yellow butt = LSI Negative turbid, purple butt = LSI Positive black precipitate = H2S Positive Atlas RM, Snyder JW (2006). (wikipedia.org)
  • The media's pH changes, transforming the colour from purple to yellow. (microbiologynote.com)
  • Glucose usage and acid generation may also cause a shift in colour, from purple to yellow. (microbiologynote.com)
  • Growth characteristics on Lowenstein-Jensen and bromcresol purple slants. (cdc.gov)
  • containing three millilitersof Bromcresol purple broth. (nih.gov)
  • In this presentation 0.3 ml. of each of the carbohydrates, glycogen 5%, starch 2% and dextrose 10% are added to tubes containing 3 ml. of bromcresol purple desoxycholate broth and incubated overnight. (nih.gov)
  • However, on bromcresol purple medium, classic M. tuber- of M. bovis . (cdc.gov)
  • If the bacteria acidify the carbohydrate, the pH will change and the indicator (brom cresol purple) will turn yellow. (cdc.gov)