An indicator and reagent. It has been used for several purposes including the determination of serum albumin concentrations
An indicator and reagent. It has been used in serum albumin determinations and as a pH indicator.
A major protein in the BLOOD. It is important in maintaining the colloidal osmotic pressure and transporting large organic molecules.
Method of analyzing chemicals using automation.
A species in the genus GARDNERELLA previously classified as Haemophilus vaginalis. This bacterium, also isolated from the female genital tract of healthy women, is implicated in the cause of bacterial vaginosis (VAGINOSIS, BACTERIAL).
The study of microorganisms such as fungi, bacteria, algae, archaea, and viruses.
Techniques used in microbiology.
Books used in the study of a subject that contain a systematic presentation of the principles and vocabulary of a subject.
Techniques used in studying bacteria.
An enzyme that catalyzes the conversion of L-alanine and 2-oxoglutarate to pyruvate and L-glutamate. (From Enzyme Nomenclature, 1992) EC 2.6.1.2.
Enzymes of the transferase class that catalyze the conversion of L-aspartate and 2-ketoglutarate to oxaloacetate and L-glutamate. EC 2.6.1.1.
A non-essential amino acid that occurs in high levels in its free state in plasma. It is produced from pyruvate by transamination. It is involved in sugar and acid metabolism, increases IMMUNITY, and provides energy for muscle tissue, BRAIN, and the CENTRAL NERVOUS SYSTEM.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
A subclass of enzymes of the transferase class that catalyze the transfer of an amino group from a donor (generally an amino acid) to an acceptor (generally a 2-keto acid). Most of these enzymes are pyridoxyl phosphate proteins. (Dorland, 28th ed) EC 2.6.1.
A family of compounds containing an oxo group with the general structure of 1,5-pentanedioic acid. (From Lehninger, Principles of Biochemistry, 1982, p442)
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Global conflict primarily fought on European continent, that occurred between 1914 and 1918.
Components of a cell produced by various separation techniques which, though they disrupt the delicate anatomy of a cell, preserve the structure and physiology of its functioning constituents for biochemical and ultrastructural analysis. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p163)
Elements of limited time intervals, contributing to particular results or situations.
A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.
Evaluation undertaken to assess the results or consequences of management and procedures used in combating disease in order to determine the efficacy, effectiveness, safety, and practicability of these interventions in individual cases or series.
That portion of the electromagnetic spectrum from the UHF (ultrahigh frequency) radio waves and extending into the INFRARED RAYS frequencies.
An allotropic form of carbon that is used in pencils, as a lubricant, and in matches and explosives. It is obtained by mining and its dust can cause lung irritation.
The spectrometric analysis of fluorescent X-RAYS, i.e. X-rays emitted after bombarding matter with high energy particles such as PROTONS; ELECTRONS; or higher energy X-rays. Identification of ELEMENTS by this technique is based on the specific type of X-rays that are emitted which are characteristic of the specific elements in the material being analyzed. The characteristic X-rays are distinguished and/or quantified by either wavelength dispersive or energy dispersive methods.
Usually a hydroxide of lithium, sodium, potassium, rubidium or cesium, but also the carbonates of these metals, ammonia, and the amines. (Grant & Hackh's Chemical Dictionary, 5th ed)
The application of electronic, computerized control systems to mechanical devices designed to perform human functions. Formerly restricted to industry, but nowadays applied to artificial organs controlled by bionic (bioelectronic) devices, like automated insulin pumps and other prostheses.
Carbonic acid calcium salt (CaCO3). An odorless, tasteless powder or crystal that occurs in nature. It is used therapeutically as a phosphate buffer in hemodialysis patients and as a calcium supplement.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
A method of measuring the effects of a biologically active substance using an intermediate in vivo or in vitro tissue or cell model under controlled conditions. It includes virulence studies in animal fetuses in utero, mouse convulsion bioassay of insulin, quantitation of tumor-initiator systems in mouse skin, calculation of potentiating effects of a hormonal factor in an isolated strip of contracting stomach muscle, etc.
An antineoplastic agent derived from BLEOMYCIN.
Water-soluble proteins found in egg whites, blood, lymph, and other tissues and fluids. They coagulate upon heating.
Any of numerous agile, hollow-horned RUMINANTS of the genus Capra, in the family Bovidae, closely related to the SHEEP.
Determination of parasite eggs in feces.
Diseases of the domestic or wild goat of the genus Capra.
A republic in southern Africa, the southernmost part of Africa. It has three capitals: Pretoria (administrative), Cape Town (legislative), and Bloemfontein (judicial). Officially the Republic of South Africa since 1960, it was called the Union of South Africa 1910-1960.
Infections by nematodes, general or unspecified.
State of the body in relation to the consumption and utilization of nutrients.
Diseases of domestic and mountain sheep of the genus Ovis.
An immunoglobulin fragment composed of one variable domain from an IMMUNOGLOBULIN HEAVY CHAIN or IMMUNOGLOBULIN LIGHT CHAIN.
Ruminant mammals of South America. They are related to camels.
Hoofed mammals with four legs, a big-lipped snout, and a humped back belonging to the family Camelidae.
Protein motif that contains a 33-amino acid long sequence that often occurs in tandem arrays. This repeating sequence of 33-amino acids was discovered in ANKYRIN where it is involved in interaction with the anion exchanger (ANION EXCHANGE PROTEIN 1, ERYTHROCYTE). Ankyrin repeats cooperatively fold into domains that mediate molecular recognition via protein-protein interactions.
The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
Persistent abnormal dilatation of the bronchi.
An autosomal recessive genetic disease of the EXOCRINE GLANDS. It is caused by mutations in the gene encoding the CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR expressed in several organs including the LUNG, the PANCREAS, the BILIARY SYSTEM, and the SWEAT GLANDS. Cystic fibrosis is characterized by epithelial secretory dysfunction associated with ductal obstruction resulting in AIRWAY OBSTRUCTION; chronic RESPIRATORY INFECTIONS; PANCREATIC INSUFFICIENCY; maldigestion; salt depletion; and HEAT PROSTRATION.
A species of gram-positive bacteria in the STREPTOCOCCUS MILLERI GROUP. It is commonly found in the oropharnyx flora and has a proclivity for abscess formation in the upper body and respiratory tract.
Material coughed up from the lungs and expectorated via the mouth. It contains MUCUS, cellular debris, and microorganisms. It may also contain blood or pus.
A species of gram-positive bacteria in the STREPTOCOCCUS MILLERI GROUP. It is the most frequently seen isolate of that group, has a proclivity for abscess formation, and is most often isolated from the blood, gastrointestinal, and urogenital tract.
A species of gram-positive bacteria in the STREPTOCOCCUS MILLERI GROUP. It is commonly found in the oropharynx flora and has a proclivity for abscess formation, most characteristically in the CENTRAL NERVOUS SYSTEM and LIVER.
Infections with bacteria of the genus PSEUDOMONAS.

Albumin as an outcome measure in haemodialysis in patients: the effect of variation in assay method. (1/23)

BACKGROUND: Serum/plasma albumin is an important predictor of future mortality/morbidity in haemodialysis (HD) patients and has been proposed as an important audit measure. Different methods of albumin assay give different results and the bias between methods may be greater in renal failure patients. METHODS: Albumin concentration in plasma was measured by three methods, two dye-binding methods (bromocresol green (BCG) and bromocresol purple (BCP)) and an immuno-turbidimetric (ITM) method, in 143 HD patients (group I) and 49 non-renal patients (group II). Comparisons were made between means, variation in differences across a range of albumin concentrations and on the percentage of patients within the normal range. RESULTS: In HD patients (group I), BCG over-estimated plasma albumin compared with the other two methods. The difference could be as much as 10 g/l and was more marked in hypoalbuminaemic patients. The BCP method gave results closer to the ITM method, particularly in HD patients. These differences were less marked in group II patients but both methods overestimated albumin compared with the ITM method. Using the BCG local laboratory normal range, 84% of HD patients had plasma albumin concentrations within the normal range but this fell to 57% if the BCP results were used. CONCLUSIONS: The method for determining albumin concentration has a marked effect on the results particularly in HD patients. BCG, the most commonly used method, gives higher results than other methods and correlates poorly with an immunological method. These differences make comparative audit between nephrology units difficult and have implications for other biochemical variables and other specialties.  (+info)

Conversion between bromcresol green- and bromcresol purple-measured albumin in renal disease. (2/23)

BACKGROUND: Albumin measured by a bromcresol purple dye-binding assay (Alb(BCP)) agrees more closely with the gold standard of immunonephelometry than does bromcresol green (Alb(BCG)) measurement. Both tests are in current clinical use. A method for converting between the two would be useful. METHODS: We measured albumin by bromcresol green and bromcresol purple in 535 patients, 155 of whom had renal disease. We randomly divided data from the patients with renal disease into two equal-sized sets, and used one set to derive, and the remaining set to validate, a regression equation relating the two values. RESULTS: The relationship Alb(BCG)=5.5+Alb(BCP) performed very well in both the renal patient validation set and in the data from 380 unselected in-patients and out-patients. Intraclass correlations for agreement between measured Alb(BCG) and predicted Alb(BCG) was 0.98 in both analyses. CONCLUSIONS: The ability to convert between these measurements will be of use in clinical situations where the absolute value of the serum albumin is important, when data from laboratories using different methodologies must be combined, and in the application of the Modification of Diet in Renal Disease formula to estimate glomerular filtration rate in patients whose albumin has been measured by bromcresol purple.  (+info)

Guidance for selecting the measurement conditions in the dye-binding method for determining serum protein: theoretical analysis based on the chemical equilibrium of protein error. (3/23)

A methodology for selecting the measurement conditions in the dye-binding method for determining serum protein has been studied by a theoretical calculation. This calculation was based on the fact that a protein error occurs because of a reaction between the side chains of a positively charged amino acid residue in a protein molecule and a dissociated dye anion. The calculated characteristics of this method are summarized as follows: (1) Although the reaction between the dye and the protein occurs up to about pH 12, a change in the color shade, called protein error, is observed only in a pH region restricted within narrow limits. (2) Although the apparent absorbance (the absorbance of the test solution measured against a reagent blank) is lower than the true absorbance indicated by the formed dye-protein complex, the apparent absorbance correlates with the true absorbance with a correlation coefficient of 1.0. (3) At a higher dye concentration, the calibration curve is more linear at a higher pH than at a lower pH. Most of these characteristics were similarly observed experimentally in the reactions of BPB, BCG and BCP with human and bovine albumins. It is concluded that in order to ensure the linearity of the calibration curve, the measurement should be performed at a higher dye concentration and sufficiently high pH where the detection sensitivity is satisfied.  (+info)

Correlation of the suicide phenomenon in Aeromonas species with virulence and enteropathogenicity. (4/23)

Certain strains of mesophilic Aeromonads like A. hydrophila, A. veronii biotype sobria and A. caviae when grown in broth containing 0.5% glucose, undergo growth inhibition concomitant with acetate accumulation. As these strains become nonviable after 24 h, this phenomenon is termed suicide. We investigated suicidal strains of Aeromonas species as means of understanding animal virulence and enteropathogenicity. Non suicidal strains of A. Hydrophila showed and overall 88.8% lethality rate and non suicidal strains of A. veronii biotype sobria showed 83.3% lethality rate and was nil for its suicidal part. Of the two suicidal A. caviae strains tested, none were lethal. The present data suggest that the suicide phenomenon may explain strain specific [A. veronii biotype sobria, A. hydrophila] and species specific [A. caviae] virulence and enteropathogenicity.  (+info)

Measurement of serum albumin by capillary zone electrophoresis, bromocresol green, bromocresol purple, and immunoassay methods. (5/23)

BACKGROUND/AIMS: The introduction of capillary zone electrophoresis (CZE) to this laboratory has highlighted discrepancies in albumin measured on an Abbott Aeroset by bromocresol green (BCG) and that calculated by CZE on the basis of total protein measured by Biuret. METHODS: This study examined differences in albumin estimation by CZE, Abbott Aeroset BCG, and Aeroset bromocresol purple (BCP), and compared these with albumin estimated by Beckman Array immunoassay. RESULTS: Altman and Bland analysis of results showed a positive bias of BCG with CZE (4.51 g/litre; 95% limits of agreement, 3.77 to 5.26; n = 72) and BCP (3.85 g/litre; 95% limits of agreement, -1.42 to 9.12; n = 72). CZE and BCP agreed closely (0.67 g/litre; 95% limits of agreement, -4.39 to 3.06; n = 72). Analysis of 57 of those samples in which BCG and CZE differed > or = 5 g/litre showed a positive bias of BCG with immunoassay (8.35 g/litre; 95% limits of agreement, 1.54 to 15.16; n =57), with good agreement between CZE and immunoassay (-0.44 g/litre; 95% limits of agreement, -2.82 to 1.94; n = 57). CONCLUSIONS: BCP is superior to BCG for the assay of albumin and has replaced BCG as the routine test for albumin in this laboratory.  (+info)

Identification of the human mitochondrial S-adenosylmethionine transporter: bacterial expression, reconstitution, functional characterization and tissue distribution. (6/23)

The mitochondrial carriers are a family of transport proteins that, with a few exceptions, are found in the inner membranes of mitochondria. They shuttle metabolites and cofactors through this membrane, and connect cytoplasmic functions with others in the matrix. SAM (S-adenosylmethionine) has to be transported into the mitochondria where it is converted into S-adenosylhomocysteine in methylation reactions of DNA, RNA and proteins. The transport of SAM has been investigated in rat liver mitochondria, but no protein has ever been associated with this activity. By using information derived from the phylogenetically distant yeast mitochondrial carrier for SAM and from related human expressed sequence tags, a human cDNA sequence was completed. This sequence was overexpressed in bacteria, and its product was purified, reconstituted into phospholipid vesicles and identified from its transport properties as the human mitochondrial SAM carrier (SAMC). Unlike the yeast orthologue, SAMC catalysed virtually only countertransport, exhibited a higher transport affinity for SAM and was strongly inhibited by tannic acid and Bromocresol Purple. SAMC was found to be expressed in all human tissues examined and was localized to the mitochondria. The physiological role of SAMC is probably to exchange cytosolic SAM for mitochondrial S-adenosylhomocysteine. This is the first report describing the identification and characterization of the human SAMC and its gene.  (+info)

Theoretical analysis concerning the characteristics of a dye-binding method for determining serum protein based on protein error of pH indicator: effect of buffer concentration of the color reagent on the color development. (7/23)

In the dye-binding method based on protein error of a pH indicator, the color development has been reported to be markedly affected by the buffer concentration of the color reagent. In this study, the author analyzed this phenomenon by a theoretical calculation based on the chemical equilibrium of protein error. The calculation was performed on the assumption that both the dissociated dye anion and the anion contained in the buffer solution react with protein, forming a dye-protein complex and an anion-protein complex, respectively. The calculated results were compared with those obtained by the experiments using bromophenol blue, bromocresol green and bromocresol purple that are employed widely for determining the human serum albumin concentration clinically. The calculated results of this method are summarized as follows: (1) the color development decreases with the increase in the concentration of the anion contained in the buffer solution; (2) the calibration curve is more linear in the higher concentration of the anion than in the lower one. These calculated results agreed well with the experimental ones. From these results, it was concluded that the change in the color development by the buffer concentration of the color reagent is due to the change in the concentration of the buffer anion.  (+info)

Measuring albumin and calcium in serum in a dual test with the Hitachi 704. (8/23)

We describe a method for simultaneously determining albumin, by using bromcresol purple, and calcium, by using Arsenazo III, in the same analytical cuvette on the Hitachi 704. Both assays agree well with accepted procedures. The standard curves for the albumin and calcium assays are linear from 0 to 60 g/L and 0 to 5.0 mmol/L, respectively. Calibration is stable for 7 days with use of open reagent in the instrument. Both assays are unaffected by hemoglobin less than or equal to 5 g/L and Intralipid less than or equal to 4 g/L; calcium is unaffected by bilirubin less than or equal to 600 mumol/L.  (+info)

Bromocresol Purple, sodium salt, pure, water soluble, indicator, ACROS Organics™ 100g; Glass bottle Bromocresol Purple, sodium salt, pure, water soluble,...
Bromocresol purple (BCP) or 5,5-dibromo-o-cresolsulfophthalein, is a pH indicator. In its sultone form, it has a pKa value of 6.3,[1] and is usually prepared as a 0.04% aqueous solution. Bromocresol purple (pH indicator) below pH 5.2 above pH 6.8 5.2 ⇌ 6.8 Uses Besides its primary function as an indicator, bromocresol purple is used in medical laboratories to measure albumin, and as an addition to acid stop baths used in photographic processing as an indicator that the bath has reached neutral pH and needs to be replaced. //////////////////////////////////////////////////////////////////// http://bmcinfectdis.biomedcentral.com/articles/10.1186/1471-2334-13-189 Strain isolation The swabs were inoculated onto MRS agar plates (Difco) and incubated at 37°C with partial CO2 tension for 48 h. Growth on MRS was observed for 21 of the 105 analysed samples, and one or more colonies were selected and picked for each of the 21 samples. All colonies were examined by Gram staining and catalase and ...
1. Anderson, N.L., et al. Cumitech 3B; Quality Systems in the Clinical Microbiology Laboratory, Coordinating ed., A.S. Weissfeld. American Society for Microbiology, Washington, D.C.. 2. Jorgensen., et al. Manual of Clinical Microbiology, American Society for Microbiology, Washington, D.C.. 3. Tille, P., et al. Bailey and Scotts Diagnostic Microbiology, C.V. Mosby Company, St. Louis, MO.. 4. Isenberg, H.D. Clinical Microbiology Procedures Handbook, Vol. I, II & III. American Society for Microbiology, Washington, D.C.. 5. Koneman, E.W., et al. Color Atlas and Textbook of Diagnostic Microbiology, J.B. Lippincott Company, Philadelphia, PA.. 6. Quality Assurance for Commercially Prepared Microbiological Culture Media, M22. Clinical and Laboratory Standards Institute (CLSI - formerly NCCLS), Wayne, PA.. ...
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Lab Reagents Elisa Reader Laboratories manufactures the elisa microplate reader reagents distributed by Genprice. The Elisa Microplate Reader reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. To purchase these products, for the MSDS, Data Sheet, protocol, storage conditions/temperature or for the concentration, please contact elisa reader. Other Elisa products are available in stock. Specificity: Elisa Category: Microplate Group: Reader Reader information ...
In the microplate based HTS field, low-volume assays, robotics, liquid handling and high-end microplate reader form a well tuned orchestra for time saving and cost efficient compound screening. To satisfy the ever-increasing demands of the HTS community, BMG LABTECH established the PHERAstar Plus multi-detection microplate reader.
MicroPlate Readers & Viewers - The MicroPlate Readers and Viewers are used to determine if there us any chemical or physical activities in a sample.
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Absorbance microplate readers and spectrophotometers for visible and UV-visible absorbance detection used for many research applications including ELISAs, nucleic acid and protein quantitation, and microbial growth.
BioTek 应用手册, 11/26 2007, Determining NADH Concentrations with Synergy™ 2 Multi-Mode Microplate Reader using Fluorescence or Absorbance
A microplate reader for detecting luminescence, florescence and absorbance, GloMax Discover offers advanced detection in a single, easy-to-use instrument. This ready-to-use multimode plate reader is developed to work seamlessly with Promega luminescence and fluorescence based detection reagents.
BMG LABTECH have developed a new gas ramping function that can fully manipulate the environment within a microplate reader, by mimicking in vitro hypoxia and ischaemia/reperfusion. Equipped with this unique feature, the CLARIOstar® with Atmospheric Control Unit (ACU) is the first plate reader that is able to rapidly return to physiological gas conditions upon active modification of oxygen (O2) and carbon dioxide (CO2) tensions within the reader, reproducing disease-specific settin
The Molecular Devices SpectraMax M2e Microplate Reader makes UV-Vis absorbance (200-1000 nm), fluorescence intensity (excitation 250-850 nm, emission 250-850 nm), chemiluminescence intensity (250-850 nm), and time-resolved fluorescence (50-1450 µsec, 200 µsec increments) measurements in 6, 12, 24, 48, 96, and 384 well microplates or in a cuvette.. ...
SpectraMax iD3 and iD5 are hybrid microplate readers with NFC functionality. The iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF, BRET, dual luciferase reporter assays with injectors, and western blot detection.
The Infinite F50 is Tecans next-generation, state-of-the-art 8-channel absorbance microplate reader that provides not only accurate, reproducible and fast measurements but sets a new standard in compact, innovative design.
BioTek 應用報告, 04/21 2003, Horseradish Peroxidase (HRP) Determination Using Amplex Red and Synergy HT Multi-Mode Microplate Reader
BioTek Note applicative, 01-Mar-05, FRET-Based Ion Channel, GPCR, and Kinase Assay Applications on the Synergy™ HT Multi-Detection Microplate Reader from BioTek
Read in this Application Note how a Microplate Reader from BMG LABTECH can measure the ENLITEN ATP Assay kit from Promega. Read more.
This webinar shows how multi-mode microplate readers are widely used in many different scientific fields including biomedical research, bioassay validation, quality control and drug discovery.
BioTek Notas de Aplicación, 21-May-08, Quantitation of Human TNF-a with a QuantiGlo Assay from R&D Systems Using BioTeks Synergy™ 2 Multi-Mode Microplate Reader - Luminescent ELISA Measurements
BioTek Notas de Aplicações, 21-May-08, Quantitation of Human TNF-a with a QuantiGlo Assay from R&D Systems Using BioTeks Synergy™ 2 Multi-Mode Microplate Reader - Luminescent ELISA Measurements
BioTek Application Notes, 28-Feb-05, Cholesterol Quantification Using a Fluorometric Assay and Synergy HT Multi-Mode Microplate Reader
In this application note, we examine how Molecular Devices SpectraMax i3x Multi- Mode Microplate Reader, in combination with CellTiter-Glo luminescent assays for cell viability and cytotoxicity.
BioTek 應用報告, 03/26 2007, cAMP and Tumor Necrosis Factor Quantification Using Synergy 2 Multi-Mode Microplate Reader and HTRF® Assays for High-Throughput Screening
BioTek アプリケーションノート, 01/15 2008, Monitoring the Growth of E. coli With Light Scattering Using the Synergy™ 4 Multi-Mode Microplate Reader with Hybrid Technology™
The Asys UVM340 Fastie-Ebert monochromator-based microplate reader has been introduced by !%Biochrom Ltd.%! The holographic grating enables users to s
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The Spark multimode microplate reader can effortlessly handle your routine or complex assay needs. Engineered in a modular, fully-upgradeable fashion.
Kevin Pfleger (University of Western Australia) talks about his experience with BRET-based ligand binding on BMG LABTECH Microplate readers. Watch here.
Microplate Reader, supplied by Tecan Systems, used in various techniques. Bioz Stars score: 99/100, based on 1406 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
BioTek Note applicative, 10-Dec-07, AlphaLISA Quantitation of Human Insulin Using a Hybrid Agilent BioTek Synergy 4 Multimode Microplate Reader
Wei Zhao, Rapid Detection of Aspergillus Fumigatus Growth Percentage by Microplate Reader, International Journal of Sciences 03(2019):1-4 DOI: 10.18483/ijSci. ...
BioTek Notes dapplication, 28-Feb-05, Cholesterol Quantification Using a Fluorometric Assay and Synergy HT Multi-Mode Microplate Reader
Ezhilkani Subbian wrote: , I am looking for a microplate reader with a small footprint that I can , use in a hood/chamber. If anyones used/seen any, please do let me know , the model/company - Thanks! to read what (absorbence, fluorescence (with or without polarisation or lifetime capability), chemoluminescence (with or without injection capability ...
Spetsiifiliste söötmete alla liigitatakse näiteks sporulatsioonisööde ning indikaatorsöötmed. Sporulatsioonisööde kujutab nälja tingimusi rakkudele. Toitainete puuduses läbivad diploidsed pärmirakud meioosi ning sporuleeruvad (spoor moodustades neli askospooriga eoskoti (eoskott). Diploidsed pagaripärmi rakud sporuleeruvad, kui nad satuvad lämmastiku- ja fermenteeritava süsiniku nälga. Pagaripärmi sporulatsioonisööde sisaldab 10 korda vähem pärmiekstrakti ja 40 korda vähem glükoosi kui rikas sööde. Indikaatorsöötmed võimaldavad kindlaks teha, kas antud tüvi on võimeline kasvama söötmesse lisatud suhkrul. Maltoosi indikaatorsööde võimaldab vahet teha tüvedel, mis fermenteerivad või ei fermenteeri maltoosi. Selleks kasutatakse rikkas söötmes süsiniku allikana maltoosi ning lisatakse värvainet bromo-kresool-punakaslilla (bromocresol purple). Maltoosi fermenteerivad rakud muudavad söötme pH-d ning koloonia ümber tekib kollane rõngas. Sööde ise aga on ...
BMG Labtech is a leading developer and a global manufacturer of microplate reader instrumentation with a wide range of measurement methods. Microplate readers are used in the pharmaceutical and biotech industries, as well as in academic research establishments, for both basic research analysis and High Throughput Screening. BMG Labtech focuses solely on microplate readers and offers the most diverse selection of optical detection systems in conjunction with integrated liquid handling equipment.. ...
The microplate extension HTS-XT is an external module that can be connected to different Bruker Optics FTIR spectrometers and allows the application of FTIR spectroscopy as method for high-throughput screening.
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Favorite Answer. tous les mots les mots exacts nimporte quels mots . afficher. By clicking Buy Now! Bromocresol Green Found 6 Item(s) Sort By Show Items Per Page Compare ( 0 ) Items Clear List View View Bromocresol Green Indicator CAS#:76-60-8 4 Product Choices Bromocresol Green… Calculate mean and standard deviation of. Faire correspondre . Bromocresol green turns one of three colors depending on the pH of the solution to which it is added. The Substance identity section is calculated from substance identification information from all ECHA databases. Contents. Buret clamp, double 1 Buret, Class A, 25-mL 1 Graduated cylinder varies1 Erlenmeyer flask, 250-mL 1 Funnel, Micro 1 Support Stand 1. 2 Answers. HACH US Chemistries CLAROS 800-227-4224. 0mL of aliquot of unknown with bromocresol green to a green endpoint Total, Calculate the hydrogen carbonate concentration, unknown mixture for each trial. Source(s): Have done the experiment. bromo cresol green. Bromocresol purples production and use ...
Different batches of bromocresol green were used in a study of several modifications of the bromocresol green method for serum albumin. No major batch variation was observed. Lyophilized animal sera and a certain batch of lyophilized human sera, however, responded differently from a pool of fresh human sera when the method was modified. These phenomena should be taken into accuont in calibration procedures and in quality assessment ...
The report generally describes sodium bromocresol green, examines its uses, production methods, patents. Sodium Bromocresol green market situation is
In this experiment, the OD value of different concentrations of Aspergillus fumigatus cultured for 48 hours was measured on a microplate reader using a 96-well plate. The absorbance-concentration curve was plotted according to the results, and the absorbance value was used instead of visual observation to quickly detect the growth of Aspergillus fumigatus. situation. The results showed that the OD value was between 0.05 and 0.06 when visually 50%, and 100% when the OD value was greater than 0.17. The use of the OD value can be a rapid method for observing the growth of Aspergillus fumigatus, which has the advantages of high efficiency, accuracy and high throughput compared with the visual method.. ...
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Make your classroom electrifying with activities and information spanning chemistry and physics content. Everything from equilibrium to electricity and reactions to rocketry at your fingertips.. ...
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As a preliminary test indicating infection, plucked hairs and skin and nail scrapings can be directly viewed under a microscope for detection of fungal elements. T. rubrum cannot be distinguished from other dermatophytes in this direct examination. It can be distinguished in vitro from other dermatophytes by means of characteristic micromorphology in culture, usually consisting of small, tear-drop-shaped microconidia, as well as its usual blood-red colony reverse pigmentation on most growth media. In addition, the Bromocresol purple (BCP) milk solid glucose agar test can be used to distinguish it. Different Trichophyton species release different amounts of ammonium ion, altering the pH of this medium. In this test, medium supporting T. rubrum remains sky blue, indicating neutral pH, until 7 to 10 days after inoculation.[6][9] In primary outgrowth on Sabouraud dextrose agar with cycloheximide and antibacterials, contaminating organisms may cause confusion, as T. rubrum colonies deprived of ...
Unit 8 Chemical Equilibrium Focusing on Acid-Base Systems unit 8Chemical Equilibrium Chemical Equilibrium Focusing on Acid Base Systems Equilibrium describes any condition or situation of balance. We recognize
Open in another window for ten minutes, the supernatant was put into check reagents and incubated at 95C within a drinking water shower for 40 a few minutes. 250 L of 0.4 M NaOH, the absorbance was measured at 450 nm using a microplate reader. Enzyme-linked immunosorbent assay (ELISA) Cells had been put into 500 L of frosty carbonate buffer (100 mM Na2 CO3, 50 mM NaCl with 11 pH.5) with protease inhibitors and homogenized by sonication. The cell lysate was centrifuged at 12,000 for 45 moments and the supernatant was utilized for dedication Limonin cost of caspase-3 content with the Caspase-3 ELISA kit (Abcam, Hong Kong, China) using a microplate reader. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining Cells were fixed with 4% paraformaldehyde at space temp (25C) for 20 moments. After washing three times with PBS, the cells were permeabilized with 1% Triton X-100 and clogged with 3% H2O2 at space temperature for ten minutes. After three washes with PBS, TdT enzyme ...
Scientists have discovered that when cells are confronted with an invading virus or bacteria or exposed to an irritating chemical, they protect themselves by going off their DNA recipe
View Notes - Equilibrium 2010 from CHEM 152 at Pima CC. Chemical Kinetics and Equilibrium Part 2: Chemical 2: Chemical Equilibrium David A. Katz Department of Chemistry Pima Community College Tucson,
In this paper we describe an approach for teaching the relation between chemical equilibrium and free energy that is, according to our experience, very efficient and enlightening. This approach has a strong visual appeal and can be used at different levels, from simple presentation of the results as graphs of free energy versus reaction mixture composition, up to full derivation of formulas to find the equations of the curves ...
A remarkable nano-confinement entropic effect on chemical equilibrium (NCECE) was predicted by us using a novel lattice-gas based statistical-mechanical modeling.
Toxicological Data on Ingredients: Methyl red LD50: Not available. Section 3: Composition / Information on Ingredients Methyl red sodium salt … Inoculate two tubes containing MR-VP Broth with a pure culture of the microorganisms under investigation. For manual titrations; indicator titrant or analyte change colour and this colour change is by far the most important method of end point detection, in such titrations. While odorless, old samples have a … Toxic gases are formed by mixing materials of this class with acids, aldehydes, amides, carbamates, cyanides, inorganic fluorides, halogenated organics, … SECTION 7: Handling and storage 7.1. IT IS GRADUALLY BEING REPLACED BY SULFONPHTHALEIN INDICATORS, SUCH AS BROMCRESOL GREEN , WHICH ARE MORE STABLE & EXHIBIT SHARPER CHANGE IN COLOR. Hazards Identification Emergency Overview----- WARNING! HMIS (0 to 4) WARNING! startxref H319-Causes serious eye irritation. Physical. Danger; Hazard Category. Oral carcinogenicity studies in rats were of a ...
specificalPrinciple of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- DbetaH antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- DbetaH antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the DbetaH amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of DbetaH can be calculated. ...
Lamin A is a component of the nuclear lamina mutated in a group of human inherited disorders known as laminopathies. B (MADB), atypical Werner syndrome (WS), familial partial lipodystrophies and metabolic laminopathies (De Sandre-Giovannoli construct. After discarding peptide and protein extra, the plates were blocked with PBS made up of 0.05% (v/v) Tween 20 and 1% (w/v) BSA for 1 hour at 37C. After washing, 100 L of Mouse monoclonal to ER immune serum diluted in PBS made up of 1% (w/v) BSA were added to each well and incubated at 37C for 1 hour. Plates were washed and an HRP-conjugated anti-rabbit antibody (Bio-Rad Laboratories) was added and incubated for 1 hour at 37C. The immune reaction was developed using 2,2-azinobis 3-ethylbenzthiazoline-6-sulfonic acid as substrate dissolved in a Colour buffer (50 mM of sodium citrate pH 3.0 with 1 l/mL AT7519 of H2O2). The absorbance at 405 nm was measured using a microplate reader (Bio-Rad Laboratories). Cell cultures Skin fibroblast cultures were ...
specificalFor samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE This AOAb ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human AOAb. This ELISA kit for research use only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAY AOAb ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AOAb antibody and an AOAb-HRP conjugate. The assay sample and buffer are incubated together with AOAb-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five time s. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is ...
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BioTeks Cytation™ Cell Imaging Multi-Mode Readers are the most advanced microplate readers on the market, delivering performance, versatility and reliability in a unique, patented design. Cytation combines automated digital upright and inverted widefield microscopy with multi-mode detection to capture both quantitative data and phenotypic information on a single platform. Cytations modularity and upgradability provides the ability to expand the system as your laboratorys applications increase. Cytation is truly ready for any assay.
TRANSIA PLATE Salmonella Gold is a sandwich enzyme immunoassay for the detection of Salmonella in food, feed and environmental samples. The test utilizes highly specific proprietary antibodies to create an antigen antibody complex which, if present, creates a color change reaction upon addition of the substrate which is read with a microplate reader. Standardized Protocol - Results in 24 hours : ...
Cytotoxicity assay. Cell viability was assessed with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (21). Cells were seeded into the 96-well microplates at 1 × 104 per well followed by IG-105 treatment at concentrations between 0 and 1 μmol/L for 72 h at 37°C. The supernatants were removed gently and cell viability was assessed. The plate was read in a microplate reader. The IC50 was determined in duplicates and each experiment was repeated at least three times under identical conditions. IC50 value was defined as the drug concentration that inhibits 50% cell growth compared with the untreated controls and calculated by regression analysis.. Pgp ATPase assay. Drug-stimulated activity of Pgp ATPase was detected by Pgp-Glo assay system (Promega). By following the user protocol provided by the vender, the activity of Pgp ATPase was measured in the presence or absence of 100 μmol/L Na3VO4, 200 μmol/L Verapamil (as a positive reference), 2 μmol/L vincristine, or 2 ...
The Human Plasminogen Activator Inhibitor-1 (PAI-1) ELISA research-use-only kit is to be used for the quantitative determination of human PAI-1 in samples (e.g., serum, EDTA and heparin plasma, cell culture supernatants, etc.) using 96-well plates and a microplate reader. The assay will recognize bo
BioTeks Synergy Neo2 Multi-Mode Microplate Reader offers even more powerful performance and assay flexibility for screening and core laboratories with an optional TRF laser. Along with dual photomultiplier tube (PMT) detection, the 337 nm laser provides the exceptional processing speed and maximum sensitivity necessary for high-quality time-resolved fluorescence (TRF) and time-resolved fluorescence resonance energy transfer (TR-FRET) results.
Assay Alkaline phosphatase in 1 hr in cell culture media, cell/tissue extracts, and biofluids with Alkaline Phosphatase Assay Kit (Fluorometric) ab83371. For microplate readers.
Assay Lipase activity in 1 hr in cell culture media, cell/tissue extracts, and biofluids with Lipase Activity Assay Kit III (Fluorometric) ab118969. For microplate readers.
BioAssay record AID 613065 submitted by ChEMBL: Activity of Staphylococcus aureus HMGR class II expressed in Escherichia coli BL21(DE3) using NADPH as substrate after 5 min by microplate reader.
To complement our Cary Eclipse fluorescence systems, we provide a portfolio of innovative fluorescence accessories. Increase the performance of your fluorescence analysis with polarizers and optical accessories, and boost your analytical capabilities with precise temperature probes or the Cary Eclipse Validation Package. For even more flexibility, we offer microplate readers, sample holders, single and multicell holders, and circulating water baths ...
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It is shown that chemical equilibrium is reached both in homogeneous and heterogeneous area of composition of reactive mixture. The liquid - liquid equilibrium data for the surface of chemical equilibrium were obtained. The thermodynamic constants of chemical equilibrium at 303.15 and 313.15 K were determined. © 2017 Elsevier B.V. ...
20 mg of the indicator bromcresol purple is added. Different types of bacteria can be differentiated on the agar by their color ... The results are scored as follows: purple slant/yellow butt = LSI Negative turbid, purple butt = LSI Positive black precipitate ... Bacteria able to decarboxylate lysine will leave the media purple colored. Bacteria producing hydrogen sulfide will appear ...
A portable fiber-optic pesticide biosensor based on immobilized cholinesterase and sol-gel entrapped bromcresol purple for in- ...
The indicator used is bromcresol purple, which in the presence of an alkaline environment will produce a purple color, while in ...
bromcresol green. *bromcresol purple. *brome grass. *brome mosaic virus. *brome mosaic virus group ...

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