An indicator and reagent. It has been used in serum albumin determinations and as a pH indicator.
An indicator and reagent. It has been used for several purposes including the determination of serum albumin concentrations
Cresols, also known as hydroxytoluene, are a group of phenolic compounds including ortho-cresol, meta-cresol, and para-cresol, which differ in the position of the hydroxyl group on the benzene ring.
A major protein in the BLOOD. It is important in maintaining the colloidal osmotic pressure and transporting large organic molecules.
Method of analyzing chemicals using automation.

Albumin as an outcome measure in haemodialysis in patients: the effect of variation in assay method. (1/38)

BACKGROUND: Serum/plasma albumin is an important predictor of future mortality/morbidity in haemodialysis (HD) patients and has been proposed as an important audit measure. Different methods of albumin assay give different results and the bias between methods may be greater in renal failure patients. METHODS: Albumin concentration in plasma was measured by three methods, two dye-binding methods (bromocresol green (BCG) and bromocresol purple (BCP)) and an immuno-turbidimetric (ITM) method, in 143 HD patients (group I) and 49 non-renal patients (group II). Comparisons were made between means, variation in differences across a range of albumin concentrations and on the percentage of patients within the normal range. RESULTS: In HD patients (group I), BCG over-estimated plasma albumin compared with the other two methods. The difference could be as much as 10 g/l and was more marked in hypoalbuminaemic patients. The BCP method gave results closer to the ITM method, particularly in HD patients. These differences were less marked in group II patients but both methods overestimated albumin compared with the ITM method. Using the BCG local laboratory normal range, 84% of HD patients had plasma albumin concentrations within the normal range but this fell to 57% if the BCP results were used. CONCLUSIONS: The method for determining albumin concentration has a marked effect on the results particularly in HD patients. BCG, the most commonly used method, gives higher results than other methods and correlates poorly with an immunological method. These differences make comparative audit between nephrology units difficult and have implications for other biochemical variables and other specialties.  (+info)

Conversion between bromcresol green- and bromcresol purple-measured albumin in renal disease. (2/38)

BACKGROUND: Albumin measured by a bromcresol purple dye-binding assay (Alb(BCP)) agrees more closely with the gold standard of immunonephelometry than does bromcresol green (Alb(BCG)) measurement. Both tests are in current clinical use. A method for converting between the two would be useful. METHODS: We measured albumin by bromcresol green and bromcresol purple in 535 patients, 155 of whom had renal disease. We randomly divided data from the patients with renal disease into two equal-sized sets, and used one set to derive, and the remaining set to validate, a regression equation relating the two values. RESULTS: The relationship Alb(BCG)=5.5+Alb(BCP) performed very well in both the renal patient validation set and in the data from 380 unselected in-patients and out-patients. Intraclass correlations for agreement between measured Alb(BCG) and predicted Alb(BCG) was 0.98 in both analyses. CONCLUSIONS: The ability to convert between these measurements will be of use in clinical situations where the absolute value of the serum albumin is important, when data from laboratories using different methodologies must be combined, and in the application of the Modification of Diet in Renal Disease formula to estimate glomerular filtration rate in patients whose albumin has been measured by bromcresol purple.  (+info)

Guidance for selecting the measurement conditions in the dye-binding method for determining serum protein: theoretical analysis based on the chemical equilibrium of protein error. (3/38)

A methodology for selecting the measurement conditions in the dye-binding method for determining serum protein has been studied by a theoretical calculation. This calculation was based on the fact that a protein error occurs because of a reaction between the side chains of a positively charged amino acid residue in a protein molecule and a dissociated dye anion. The calculated characteristics of this method are summarized as follows: (1) Although the reaction between the dye and the protein occurs up to about pH 12, a change in the color shade, called protein error, is observed only in a pH region restricted within narrow limits. (2) Although the apparent absorbance (the absorbance of the test solution measured against a reagent blank) is lower than the true absorbance indicated by the formed dye-protein complex, the apparent absorbance correlates with the true absorbance with a correlation coefficient of 1.0. (3) At a higher dye concentration, the calibration curve is more linear at a higher pH than at a lower pH. Most of these characteristics were similarly observed experimentally in the reactions of BPB, BCG and BCP with human and bovine albumins. It is concluded that in order to ensure the linearity of the calibration curve, the measurement should be performed at a higher dye concentration and sufficiently high pH where the detection sensitivity is satisfied.  (+info)

Screening for albuminuria: a case for estimation of albumin in urine. (4/38)

The usefulness of bromcresol green for estimating albumin in urine was evaluated by comparison with the Laurell "rocket" technique. In contrast to the bromcresol green method applied for urinary albumin, rather doubtful results were obtained with conventional (Microzone) electrophoresis for albumin and with precipitation techniques for total protein estimation. Albumin estimation with bromcresol green is recommended as a more reliable substitute for total-protein estimations in urine. Limitations of bromcresol green are also pointed out.  (+info)

Expression of PGT in MDCK cell monolayers: polarized apical localization and induction of active PG transport. (5/38)

The PG transporter (PGT) is expressed in subapical vesicles in the kidney collecting duct. To gain insight into the possible function of the PGT in this tubule segment, we tagged rat PGT with green fluorescent protein at the COOH terminus and generated stable PGT-expressing Madin-Darby canine kidney cell lines. When grown on permeable filters, green fluorescent protein-PGT was expressed predominantly at the apical membrane. Although the basal-to-apical transepithelial flux of [(3)H]PGE(2) was little changed by PGT expression, the apical-to-basolateral flux was increased 100-fold compared with wild-type cells. Analysis of driving forces revealed that this flux represents PGT-mediated active transepithelial PGE(2) transport. We propose that endogenous PGT is exocytically inserted into the collecting duct apical membrane, where it could control the concentration of luminal PGs.  (+info)

Measurement of serum albumin by capillary zone electrophoresis, bromocresol green, bromocresol purple, and immunoassay methods. (6/38)

BACKGROUND/AIMS: The introduction of capillary zone electrophoresis (CZE) to this laboratory has highlighted discrepancies in albumin measured on an Abbott Aeroset by bromocresol green (BCG) and that calculated by CZE on the basis of total protein measured by Biuret. METHODS: This study examined differences in albumin estimation by CZE, Abbott Aeroset BCG, and Aeroset bromocresol purple (BCP), and compared these with albumin estimated by Beckman Array immunoassay. RESULTS: Altman and Bland analysis of results showed a positive bias of BCG with CZE (4.51 g/litre; 95% limits of agreement, 3.77 to 5.26; n = 72) and BCP (3.85 g/litre; 95% limits of agreement, -1.42 to 9.12; n = 72). CZE and BCP agreed closely (0.67 g/litre; 95% limits of agreement, -4.39 to 3.06; n = 72). Analysis of 57 of those samples in which BCG and CZE differed > or = 5 g/litre showed a positive bias of BCG with immunoassay (8.35 g/litre; 95% limits of agreement, 1.54 to 15.16; n =57), with good agreement between CZE and immunoassay (-0.44 g/litre; 95% limits of agreement, -2.82 to 1.94; n = 57). CONCLUSIONS: BCP is superior to BCG for the assay of albumin and has replaced BCG as the routine test for albumin in this laboratory.  (+info)

Theoretical analysis concerning the characteristics of a dye-binding method for determining serum protein based on protein error of pH indicator: effect of buffer concentration of the color reagent on the color development. (7/38)

In the dye-binding method based on protein error of a pH indicator, the color development has been reported to be markedly affected by the buffer concentration of the color reagent. In this study, the author analyzed this phenomenon by a theoretical calculation based on the chemical equilibrium of protein error. The calculation was performed on the assumption that both the dissociated dye anion and the anion contained in the buffer solution react with protein, forming a dye-protein complex and an anion-protein complex, respectively. The calculated results were compared with those obtained by the experiments using bromophenol blue, bromocresol green and bromocresol purple that are employed widely for determining the human serum albumin concentration clinically. The calculated results of this method are summarized as follows: (1) the color development decreases with the increase in the concentration of the anion contained in the buffer solution; (2) the calibration curve is more linear in the higher concentration of the anion than in the lower one. These calculated results agreed well with the experimental ones. From these results, it was concluded that the change in the color development by the buffer concentration of the color reagent is due to the change in the concentration of the buffer anion.  (+info)

Inhibitors of prostaglandin transport and metabolism augment protease-activated receptor-2-mediated increases in prostaglandin E2 levels and smooth muscle relaxation in mouse isolated trachea. (8/38)

Stimulants of protease-activated receptor-2 (PAR(2)), such as Ser-Leu-Ile-Gly-Arg-Leu-NH(2) (SLIGRL), cause airway smooth muscle relaxation via the release of the bronchodilatory prostanoid prostaglandin E(2) (PGE(2)). The principal aim of the current study was to determine whether compounds that inhibit PGE(2) reuptake by the prostaglandin transporter [bromocresol green and U46619 (9,11-dideoxy-9alpha,11alpha-methanoepoxy PGF2alpha) and PGE(2) metabolism by 15-hydroxyprostaglandin dehydrogenase (thiazolidenedione compounds rosiglitazone and ciglitazone) significantly enhanced the capacity of SLIGRL to elevate PGE(2) levels and produce relaxation in isolated segments of upper and lower mouse trachea. SLIGRL produced concentration-dependent increases in PGE(2) levels and smooth muscle relaxation, although both effects were significantly greater in lower tracheal segments than in upper tracheal segments. SLIGRL-induced increases in PGE(2) levels were significantly enhanced in the presence of ciglitazone and rosiglitazone, and these effects were not inhibited by GW9662 (2-chloro-5-nitrobenzanilide), a peroxisome proliferator-activated receptor-gamma antagonist. SLI-GRL-induced relaxation responses were also significantly enhanced by ciglitazone and rosiglitazone, whereas responses to isoprenaline, a PGE(2)-independent smooth muscle relaxant, were unaltered. Ciglitazone and rosiglitazone alone produced concentration-dependent increases in PGE(2) levels and smooth muscle relaxation, and these responses were inhibited by indomethacin, a cyclooxygenase inhibitor. Bromocresol green, an inhibitor of prostaglandin transport, significantly enhanced SLIGRL-induced increases in PGE(2) levels and relaxation. Immunohistochemical staining for 15-hydroxyprostaglandin dehydrogenase was relatively intense over airway smooth muscle, as was staining for the prostaglandin transporter over both airway smooth muscle and epithelium. In summary, inhibitors of PGE(2) reuptake and metabolism significantly potentiate PAR(2)-mediated increases in PGE(2) levels and smooth muscle relaxation in murine-isolated airways.  (+info)

Bromcresol green is a pH indicator dye that is commonly used in medical and laboratory settings to determine the acidity or alkalinity (pH level) of various substances. It has a green color in its unionized form, which appears at a pH range of 3.8 to 5.4. When the pH rises above 5.4, bromcresol green turns blue, indicating an alkaline environment.

In medical testing, bromcresol green is often used in urinalysis and other bodily fluid analysis to assess acid-base balance. It can also be used as a component of certain culture media for microbiological tests. However, it's worth noting that bromcresol green has been largely replaced by other pH indicators like phenolphthalein and methyl orange in many applications due to its lower sensitivity and specificity.

Bromcresol purple is a pH indicator dye that is commonly used in medical and laboratory settings to determine the acidity or alkalinity (pH level) of various substances. It is often used in clinical chemistry to monitor the pH of blood, urine, and other bodily fluids.

When added to a solution, bromcresol purple changes color depending on the pH of the solution. At a pH below 5.2, it appears yellow, while at a pH above 6.8, it turns purple. In between these values, it takes on various shades of greenish-blue, with the exact shade corresponding to the pH level of the solution.

Bromcresol purple is also used in some types of litmus paper and in certain medical tests, such as the bromcresol purple test for proteinuria (the presence of excess protein in the urine), where it can help detect changes in the pH of urine that may indicate kidney disease or other health problems.

Cresols are a group of chemical compounds that are phenolic derivatives of benzene, consisting of methyl substituted cresidines. They have the formula C6H4(OH)(\_3CH3). There are three isomers of cresol, depending on the position of the methyl group: ortho-cresol (m-cresol), meta-cresol (p-cresol), and para-cresol (o-cresol). Cresols are used as disinfectants, antiseptics, and preservatives in various industrial and commercial applications. They have a characteristic odor and are soluble in alcohol and ether. In medical terms, cresols may be used as topical antiseptic agents, but they can also cause skin irritation and sensitization.

Serum albumin is the most abundant protein in human blood plasma, synthesized by the liver. It plays a crucial role in maintaining the oncotic pressure or colloid osmotic pressure of blood, which helps to regulate the fluid balance between the intravascular and extravascular spaces.

Serum albumin has a molecular weight of around 66 kDa and is composed of a single polypeptide chain. It contains several binding sites for various endogenous and exogenous substances, such as bilirubin, fatty acids, hormones, and drugs, facilitating their transport throughout the body. Additionally, albumin possesses antioxidant properties, protecting against oxidative damage.

Albumin levels in the blood are often used as a clinical indicator of liver function, nutritional status, and overall health. Low serum albumin levels may suggest liver disease, malnutrition, inflammation, or kidney dysfunction.

"Autoanalysis" is not a term that is widely used in the medical field. However, in psychology and psychotherapy, "autoanalysis" refers to the process of self-analysis or self-examination, where an individual analyzes their own thoughts, feelings, behaviors, and experiences to gain insight into their unconscious mind and understand their motivations, conflicts, and emotional patterns.

Self-analysis can involve various techniques such as introspection, journaling, meditation, dream analysis, and reflection on past experiences. While autoanalysis can be a useful tool for personal growth and self-awareness, it is generally considered less reliable and comprehensive than professional psychotherapy or psychoanalysis, which involves a trained therapist or analyst who can provide objective feedback, interpretation, and guidance.

... bromcresol green MeSH D02.455.426.559.389.832.150 - bromcresol purple MeSH D02.455.426.559.389.832.180 - bromphenol blue MeSH ... bromcresol green MeSH D02.886.590.887.150 - bromcresol purple MeSH D02.886.590.887.180 - bromphenol blue MeSH D02.886.590.887. ... bromcresol green MeSH D02.755.239.195 - bromcresol purple MeSH D02.755.239.216 - butylated hydroxytoluene MeSH D02.755.239.327 ... lissamine green dyes MeSH D02.092.877.648 - oxyphenonium MeSH D02.092.877.674 - phenylammonium compounds MeSH D02.092.877.674. ...
A portable fiber-optic pesticide biosensor based on immobilized cholinesterase and sol-gel entrapped bromcresol purple for in- ... Fret sensor detected by means of time-resolved/nanotomography allophycocyanin-ConA/malachite green-Dextran, regarding Fret with ... made a NIR Fret sensor measuring both the time-resolved counts or by nanotomography of allophycocyanin-ConA/malachite green- ...
bromcresol green *Malaise. *budesonide *Malaise. *buprenorphine *Malaise. *bupropion *Malaise: postmarketing, rare *buspirone * ...
... bromcresol green MeSH D02.455.426.559.389.832.150 - bromcresol purple MeSH D02.455.426.559.389.832.180 - bromphenol blue MeSH ... bromcresol green MeSH D02.886.590.887.150 - bromcresol purple MeSH D02.886.590.887.180 - bromphenol blue MeSH D02.886.590.887. ... bromcresol green MeSH D02.755.239.195 - bromcresol purple MeSH D02.755.239.216 - butylated hydroxytoluene MeSH D02.755.239.327 ... lissamine green dyes MeSH D02.092.877.648 - oxyphenonium MeSH D02.092.877.674 - phenylammonium compounds MeSH D02.092.877.674. ...
Although BCP is structurally similar to the conventional Bromcresol green (BCG), its pH color change interval is higher (5.2 - ... At the reaction pH, the Bromcresol purple (BCP) in the Boehringer Mannheim Diagnostics (BMD) albumin system reagent binds ...
bromcresol green reagent (Smith, 1960),. 0·1% acridine in absolute ethanol followed by examination of papers under UV light ( ...
"Novel Fiber-Optic Biosensor Based on Immobilized Glutathione S-Trans-ferase and Sol-Gel Entrapped Bromcresol Green for the ...
Bromcresol Green (selective for Albumin), Fluorescamine (selective for primary amino groups) and the application of proteases. ...
D. Bromcresol green. Ans.B. 40. A solution of known concentration is the definition of a. A. Buffer solution.. B. Neutral ...
4.0 g/dl for the bromcresol green laboratory method) to ensure optimal nutrition and decrease risks of protein-energy wasting"[ ...
Brilliant Green Agar: selective medium for the isolation of Salmonella, except S. typhi.. Brilliant green is the selective ... Bromcresol purple is the indicator.. Simmons Citrate Agar: utilization of citrate as the sole source of carbon is indicated by ... Bromcresol purple is the pH indicator.. MacConkey Agar: an inhibitory and differential medium used to distinguish lactose- ... colonies appear green. Thayer-Martin Agar: chocolate agar supplemented with antibiotic inhibitors for selective isolation of ...
Cyclohehimide, ethanol or the dye brom-cresol-green were used as inhibitors and galactose as a selective carbon source. There ... The BIL glucose-cyclohehimide and BIL brom-cresol-green media favored isolation of more species diversity. The high frequency ...
Albumin standards and the measurement of serum albumin with bromcresol green. Clin Chim Acta. 1971;31(1):87-96. ... Albumin concentrations were determined according to the principles based on the bromocresol green reaction [31] respectively. ...
Bromcresol Green. *Bromcresol Purple. *Butylated Hydroxytoluene. *Creosote. *Cyclofenil. *Dinitrocresols. *Formocresols. * ...
Albumin standards and the measurement of serum albumin with bromcresol green. Clinica chimica acta, 31(1), 87-96. ...
It contains Bromcresol Green, a chemical ink that changes colour when applied to paper. A yellow dot indicates high acid ... content, green dot indicates some acid and blue dot indicates no acid. ...
Sebutkan fungsi dari Albumin Metode biuret dan bromcresol green Sebagai pengikat lemak dalam darah dan menjaga onkotik pd. SOAL ...
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The mechanism in unclear and could be due to pH changes altering binding of the bromcresol green dye to the protein. ... albumin + bromocrescol green pH 4.1 , blue-green complex. Units of measurement. Albumin concentration is measured in g/dL ( ... however fibrinogen and other acute phase proteins may bind to bromcresol green resulting in higher concentrations in heparin ... Bromocresol green (BCG) method: At an acidic pH of 4.1 albumin is significantly charged allowing it to bind to the anionic dye ...
BROM CRESOL GREEN 1988-04-01 * Brom-O-Cresol Sulfone Pthalein 2022-01-12 ...
... consists in titration of a test portion with a standard volumetric solution of sodium hydroxide in presence of bromcresol green ...
... measured by bromcresol green method) of 3.7 g/dL or less for men and 3.5 g/dL or less for women; and/or (3) evidence of ...
Leading products are thymolphthalein, o-Cresolphthalein, phenanthroline, bromophenol blue, bromocresol green, bromcresol purple ...
Subsequently, the diluted broth of number 58 and 103 were spread on the solid plates with bromcresol green, and 59 single ... The pH indicator (bromocresol green) assay in 24-well MTPs was a qualitative method for organic acid concentration in ... and 100 μl each of diluted suspension was inoculated into 24-well MTPs and cultured for 24 h with 60 mg/l of bromocresol green ... presenting good performances on the production of organic acid were further spread on the solid plates with bromocresol green ...
D2.455.426.559.389.657.239.180 Bromcresol Green D2.755.239.190 D2.455.426.559.389.657.239.190 Bromcresol Purple D2.755.239.195 ...
D2.455.426.559.389.657.239.180 Bromcresol Green D2.755.239.190 D2.455.426.559.389.657.239.190 Bromcresol Purple D2.755.239.195 ...
D2.455.426.559.389.657.239.180 Bromcresol Green D2.755.239.190 D2.455.426.559.389.657.239.190 Bromcresol Purple D2.755.239.195 ...
D2.455.426.559.389.657.239.180 Bromcresol Green D2.755.239.190 D2.455.426.559.389.657.239.190 Bromcresol Purple D2.755.239.195 ...
D2.455.426.559.389.657.239.180 Bromcresol Green D2.755.239.190 D2.455.426.559.389.657.239.190 Bromcresol Purple D2.755.239.195 ...
D2.455.426.559.389.657.239.180 Bromcresol Green D2.755.239.190 D2.455.426.559.389.657.239.190 Bromcresol Purple D2.755.239.195 ...
D2.455.426.559.389.657.239.180 Bromcresol Green D2.755.239.190 D2.455.426.559.389.657.239.190 Bromcresol Purple D2.755.239.195 ...
  • Albumin standards and the measurement of serum albumin with bromcresol green. (edu.ly)
  • Albumin is measured by its ability to bind to bromocresol green. (eclinpath.com)
  • There are also species peculiarities in the amount of bromocresol green that binds to albumin. (eclinpath.com)
  • At an acidic pH of 4.1 albumin is significantly charged allowing it to bind to the anionic dye bromocresol green forming a blue-green complex. (eclinpath.com)
  • The results indicated that although lysine and histidine residues were modified, such modification did not alter the functions or biological properties of albumin, such as binding to palmitic-acid or bromcresol-green. (cdc.gov)
  • Bromcresol purple is the pH indicator. (umd.edu)
  • The principle consists in titration of a test portion with a standard volumetric solution of sodium hydroxide in presence of bromcresol green as indicator. (iso.org)
  • DNase Test Agar, DNase Test Agar with Methyl Green and DNase Test Agar with Toluidine Blue are differential media used for the detection of deoxyribonuclease activity to aid in the identification of bacteria isolated from clinical specimens. (slideserve.com)
  • The HCl reagent is not needed to detect DNase activity on DNase Agar with Methyl Green. (slideserve.com)
  • A yellow dot indicates high acid content, green dot indicates some acid and blue dot indicates no acid. (archivalsurvival.com.au)
  • Methyl green forms a complex with intact (polymerized) DNAto form the green color of the medium. (slideserve.com)
  • DNase activity depolymerizes the DNA, breaking down the methyl green-DNA complex, which results in the formation of colorless zones around colonies of the test organism. (slideserve.com)
  • Bromcresol green and chlorphenol red papers can be used as criteria if the solution is allowed to creep into the paper so as to give an indicator zone ahead of the bleached zone. (sciencemadness.org)
  • A yellow dot indicates high acid content, green dot indicates some acid and blue dot indicates no acid. (archivalsurvival.com.au)
  • Influence of plasma protein on the inhibitory effects of indocyanine green and bromcresol green on pulmonary prostaglandin E1 extraction. (nih.gov)