A BRAIN-specific hyalectin that may play a role in terminally differentiating NEURONS. It is found highly overexpressed in primary BRAIN TUMORS and in experimental models of GLIOMA.
Proteoglycans consisting of proteins linked to one or more CHONDROITIN SULFATE-containing oligosaccharide chains.
A class of animal lectins that bind to carbohydrate in a calcium-dependent manner. They share a common carbohydrate-binding domain that is structurally distinct from other classes of lectins.
An extracellular endopeptidase which excises a block of peptides at the amino terminal, nonhelical region of the procollagen molecule with the formation of collagen. Absence or deficiency of the enzyme causes accumulation of procollagen which results in the inherited connective tissue disorder--dermatosparaxis. EC 3.4.24.14.
HYALURONAN-containing proteoglycans found in the EXTRACELLULAR MATRIX of a variety of tissues and organs. Several versican isoforms exist due to multiple ALTERNATIVE SPLICING of the versican MESSENGER RNA.
A family of polypeptides purified from snake venoms, which contain the arginine-glycine-aspartic acid (RGD) sequence. The RGD tripeptide binds to integrin receptors and thus competitively inhibits normal integrin-ligand interactions. Disintegrins thus block adhesive functions and act as platelet aggregation inhibitors.
A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere.
A plastic surgical operation on the nose, either reconstructive, restorative, or cosmetic. (Dorland, 28th ed)
Macromolecular organic compounds that contain carbon, hydrogen, oxygen, nitrogen, and usually, sulfur. These macromolecules (proteins) form an intricate meshwork in which cells are embedded to construct tissues. Variations in the relative types of macromolecules and their organization determine the type of extracellular matrix, each adapted to the functional requirements of the tissue. The two main classes of macromolecules that form the extracellular matrix are: glycosaminoglycans, usually linked to proteins (proteoglycans), and fibrous proteins (e.g., COLLAGEN; ELASTIN; FIBRONECTINS; and LAMININ).
A non-vascular form of connective tissue composed of CHONDROCYTES embedded in a matrix that includes CHONDROITIN SULFATE and various types of FIBRILLAR COLLAGEN. There are three major types: HYALINE CARTILAGE; FIBROCARTILAGE; and ELASTIC CARTILAGE.
A protective layer of firm, flexible cartilage over the articulating ends of bones. It provides a smooth surface for joint movement, protecting the ends of long bones from wear at points of contact.
Glycoproteins found on the surfaces of cells, particularly in fibrillar structures. The proteins are lost or reduced when these cells undergo viral or chemical transformation. They are highly susceptible to proteolysis and are substrates for activated blood coagulation factor VIII. The forms present in plasma are called cold-insoluble globulins.
A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).
Benign and malignant central nervous system neoplasms derived from glial cells (i.e., astrocytes, oligodendrocytes, and ependymocytes). Astrocytes may give rise to astrocytomas (ASTROCYTOMA) or glioblastoma multiforme (see GLIOBLASTOMA). Oligodendrocytes give rise to oligodendrogliomas (OLIGODENDROGLIOMA) and ependymocytes may undergo transformation to become EPENDYMOMA; CHOROID PLEXUS NEOPLASMS; or colloid cysts of the third ventricle. (From Escourolle et al., Manual of Basic Neuropathology, 2nd ed, p21)
A natural high-viscosity mucopolysaccharide with alternating beta (1-3) glucuronide and beta (1-4) glucosaminidic bonds. It is found in the UMBILICAL CORD, in VITREOUS BODY and in SYNOVIAL FLUID. A high urinary level is found in PROGERIA.
Acidic sulfated integral membrane glycoproteins expressed in several alternatively spliced and variable glycosylated forms on a wide variety of cell types including mature T-cells, B-cells, medullary thymocytes, granulocytes, macrophages, erythrocytes, and fibroblasts. CD44 antigens are the principle cell surface receptors for hyaluronate and this interaction mediates binding of lymphocytes to high endothelial venules. (From Abbas et al., Cellular and Molecular Immunology, 2d ed, p156)
Neoplasms of the intracranial components of the central nervous system, including the cerebral hemispheres, basal ganglia, hypothalamus, thalamus, brain stem, and cerebellum. Brain neoplasms are subdivided into primary (originating from brain tissue) and secondary (i.e., metastatic) forms. Primary neoplasms are subdivided into benign and malignant forms. In general, brain tumors may also be classified by age of onset, histologic type, or presenting location in the brain.
Libraries in which a major proportion of the resources are available in machine-readable format, rather than on paper or MICROFORM.
The systematic study of the structure and function of the complete set of glycans (the glycome) produced in a single organism and identification of all the genes that encode glycoproteins.
Heteropolysaccharides which contain an N-acetylated hexosamine in a characteristic repeating disaccharide unit. The repeating structure of each disaccharide involves alternate 1,4- and 1,3-linkages consisting of either N-acetylglucosamine or N-acetylgalactosamine.
Books used in the study of a subject that contain a systematic presentation of the principles and vocabulary of a subject.
Derivatives of chondroitin which have a sulfate moiety esterified to the galactosamine moiety of chondroitin. Chondroitin sulfate A, or chondroitin 4-sulfate, and chondroitin sulfate C, or chondroitin 6-sulfate, have the sulfate esterified in the 4- and 6-positions, respectively. Chondroitin sulfate B (beta heparin; DERMATAN SULFATE) is a misnomer and this compound is not a true chondroitin sulfate.
Inorganic salts of sulfuric acid.
Individuals enrolled in a school or formal educational program.
Copies of a work or document distributed to the public by sale, rental, lease, or lending. (From ALA Glossary of Library and Information Science, 1983, p181)
Individuals enrolled in a school of medicine or a formal educational program in medicine.
The profession of writing. Also the identity of the writer as the creator of a literary production.
"The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.

The proteoglycan lectin domain binds sulfated cell surface glycolipids and promotes cell adhesion. (1/43)

The lecticans are a group of chondroitin sulfate proteoglycans characterized by the presence of C-type lectin domains. Despite the suggestion that their lectin domains interact with carbohydrate ligands, the identity of such ligands has not been elucidated. We previously showed that brevican, a nervous system-specific lectican, binds the surface of B28 glial cells (Yamada, H., Fredette, B., Shitara, K., Hagihara, K., Miura, R., Ranscht, B., Stallcup, W. B., and Yamaguchi, Y. (1997) J. Neurosci. 17, 7784-7795). In this paper, we demonstrate that two classes of sulfated glycolipids, sulfatides and HNK-1-reactive sulfoglucuronylglycolipids (SGGLs), act as cell surface receptors for brevican. The lectin domain of brevican binds sulfatides and SGGLs in a calcium-dependent manner as expected of a C-type lectin domain. Intact, full-length brevican also binds both sulfatides and SGGLs. The lectin domain immobilized as a substrate supports adhesion of cells expressing SGGLs or sulfatides, which was inhibited by monoclonal antibodies against these glycolipids or by treatment of the substrate with SGGLs or sulfatides. Our findings demonstrate that the interaction between the lectin domains of lecticans and sulfated glycolipids comprises a novel cell substrate recognition system, and suggest that lecticans in extracellular matrices serve as substrate for adhesion and migration of cells expressing these glycolipids in vivo.  (+info)

Bovine CNS myelin contains neurite growth-inhibitory activity associated with chondroitin sulfate proteoglycans. (2/43)

The absence of fiber regrowth in the injured mammalian CNS is influenced by several different factors and mechanisms. Besides the nonconducive properties of the glial scar tissue that forms around the lesion site, individual molecules present in CNS myelin and expressed by oligodendrocytes, such as NI-35/NI-250, bNI-220, and myelin-associated glycoprotein (MAG), have been isolated and shown to inhibit axonal growth. Here, we report an additional neurite growth-inhibitory activity purified from bovine spinal cord myelin that is not related to bNI-220 or MAG. This activity can be ascribed to the presence of two chondroitin sulfate proteoglycans (CSPGs), brevican and the brain-specific versican V2 splice variant. Neurite outgrowth of neonatal cerebellar granule cells and of dorsal root ganglion neurons in vitro was strongly inhibited by this myelin fraction enriched in CSPGs. Immunohistochemical staining revealed that brevican and versican V2 are present on the surfaces of differentiated oligodendrocytes. We provide evidence that treatment of oligodendrocytes with the proteoglycan synthesis inhibitors beta-xylosides can strongly influence the growth permissiveness of oligodendrocytes. beta-Xylosides abolished cell surface presentation of brevican and versican V2 and reversed growth cone collapse in encounters with oligodendrocytes as demonstrated by time-lapse video microscopy. Instead, growth cones were able to grow along or even into the processes of oligodendrocytes. Our results strongly suggest that brevican and versican V2 are additional components of CNS myelin that contribute to its nonpermissive substrate properties for axonal growth. Expression of these CSPGs on oligodendrocytes may indicate that they participate in the restriction of structural plasticity and regeneration in the adult CNS.  (+info)

The chondroitin sulfate proteoglycans neurocan and phosphacan are expressed by reactive astrocytes in the chronic CNS glial scar. (3/43)

Chondroitin sulfate proteoglycans (CS-PGs) expressed by reactive astrocytes may contribute to the axon growth-inhibitory environment of the injured CNS. The specific potentially inhibitory CS-PGs present in areas of reactive gliosis, however, have yet to be thoroughly examined. In this study, we used immunohistochemistry, combined immunohistochemistry-in situ hybridization, immunoblot analysis, and reverse transcription-PCR to examine the expression of specific CS-PGs by reactive astrocytes in an in vivo model of reactive gliosis: that is, the glial scar, after cortical injury. Neurocan and phosphacan can be localized to reactive astrocytes 30 d after CNS injury, whereas brevican and versican are not expressed in the chronic glial scar. Neurocan is also expressed by astrocytes in primary cell culture. Relative to the amount present in cultured astrocytes or uninjured cortex, neurocan expression increases significantly in the glial scar resulting from cortical injury, including the re-expression of the neonatal isoform of neurocan. In contrast, phosphacan protein levels are decreased in the glial scar compared with the uninjured brain. Because these CS-PGs are capable of inhibiting neurite outgrowth in vitro, our data suggest that phosphacan and neurocan in areas of reactive gliosis may contribute to axonal regenerative failure after CNS injury.  (+info)

A peptide mimic of E-selectin ligand inhibits sialyl Lewis X-dependent lung colonization of tumor cells. (4/43)

Selectins bind to carbohydrate ligands in a calcium-dependent manner and play critical roles in host defense and possibly in tumor metastasis. To isolate peptides that mimic E-selectin ligands, we screened a phage peptide library using E-selectin as a target molecule. This attempt unexpectedly failed, probably because the binding affinity of E-selectin to its ligand is low. We then took an approach that is analogous to the isolation of anti-idiotype antibodies and were able to isolate peptides that bound to anticarbohydrate antibodies recognizing E-selectin ligands. These peptides, enriched for their binding to anti-Lewis A antibody, were found to bind to E-, P- and L-selectins in a calcium-dependent manner. Phage harboring the identified peptide IELLQAR and synthetic peptides having the same sequence inhibited the binding of sialyl Lewis X or sialyl Lewis A oligosaccharides to E-selectin. The adhesion of HL-60 and B16 melanoma cells expressing sialyl Lewis X to E-selectin was also inhibited by the phage-displaying IELLQAR peptide. Moreover, i.v. injected IELLQAR peptide inhibited the lung colonization of mouse B16 melanoma and human lung tumor cells expressing sialyl Lewis X. These results demonstrate that it is possible to isolate peptides mimicking carbohydrate ligands by screening the peptides for binding to anticarbohydrate antibodies and then using them to inhibit carbohydrate-dependent experimental tumor metastasis.  (+info)

Brain-enriched hyaluronan binding (BEHAB)/brevican cleavage in a glioma cell line is mediated by a disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS) family member. (5/43)

Brain-enriched hyaluronan binding (BEHAB)/brevican is a brain-specific extracellular matrix protein containing a cleavage site between Glu(395)-Ser(396), which bears remarkable homology to the "aggrecanase" site in the cartilage proteoglycan aggrecan. Expression of BEHAB/brevican is dramatically increased in human gliomas, notoriously invasive tumors. Recently, we showed that the rat 9L gliosarcoma cell line, which does not express BEHAB/brevican and forms non-invasive tumors when grown as intracranial grafts, can form invasive tumors when transfected with a 5' cDNA fragment of BEHAB/brevican, but not when transfected with the full-length cDNA. In marked contrast, the highly invasive CNS-1 glioma cell line expresses and cleaves BEHAB/brevican protein when grown as an intracranial graft. These results suggest that both synthesis and cleavage of BEHAB/brevican protein may play a role in the invasiveness of gliomas. We report here, using an antibody developed to the neoepitope created by BEHAB/brevican cleavage at the Glu(395)-Ser(396) site, that the CNS-1 cells are able to cleave the protein in vitro. We characterized the CNS-1-derived cleavage activity by assaying its ability to cleave BEHAB/brevican proteoglycan, and determined that the enzyme is a constitutively expressed, secreted activity. Using a variety of protease inhibitors, reverse transcriptase-polymerase chain reaction, and specific antibodies, we determined that this activity is likely to be a member of the ADAMTS family of metalloproteinases, specifically ADAMTS4. These results suggest a novel function for ADAMTS family members in BEHAB/brevican cleavage and glioma and indicate that inhibition of ADAMTS in glioma may provide a novel therapeutic strategy.  (+info)

Brevican is degraded by matrix metalloproteinases and aggrecanase-1 (ADAMTS4) at different sites. (6/43)

Brevican is a member of the lectican family of chondroitin sulfate proteoglycans that is predominantly expressed in the central nervous system. The susceptibility of brevican to digestion by matrix metalloproteinases (MMP-1, -2, -3, -7, -8, -9, -10, and -13 and membrane type 1 and 3 MMPs) and aggrecanase-1 (ADAMTS4) was examined. MMP-1, -2, -3, -7, -8, -10, and -13 degraded brevican into a few fragments with similar molecular masses, whereas the degradation products of aggrecanase-1 had apparently different sizes. NH(2)-terminal sequence analyses of the digestion fragments revealed that cleavages of the brevican core protein by these metalloproteinases occurred commonly within the central non-homologous domain. MMP-1, -2, -3, -7, -8, -10, and -13 preferentially attacked the Ala(360)-Phe(361) bond, whereas aggrecanase-1 cleaved the Glu(395)-Ser(396) bond, which are similar to the cleavage sites observed with cartilage proteoglycan (aggrecan) for the MMPs and aggrecanase-1, respectively. These data demonstrate that MMP-1, -2, -3, -7, -8, -10, and -13 and aggrecanase-1 digest brevican in a similar pattern to aggrecan and suggest that they may be responsible for the physiological turnover and pathological degradation of brevican.  (+info)

Xenopus brevican is expressed in the notochord and the brain during early embryogenesis. (7/43)

A complete cDNA encoding the Xenopus laevis homologue of the aggrecan/versican family member, brevican (Xbcan) was cloned from an embryonic stage 42 cDNA library. In the deduced amino acid sequence, 1152 in length, similarity to the hyaluronan-binding (link) domains of brevicans from other species were present in the N-terminal region as well as EGF-, lectin- and complement regulatory protein-like domains in the C-terminal part, the latter three being characteristic for brevican found within the extracellular matrix (J. Biol. Chem. 269 (1994) 10119). Indeed, Xbcan was secreted into the extracellular space as a soluble protein when expressed in oocytes. No cDNAs encoding a GPI-anchored bcan variant could be isolated from that cDNA library. During embryonic development, the expression of this gene was first observed in the notochord of neurula stage embryos. In addition to this, in tailbuds, Xbcan was also found to be expressed within the fifth and sixth rhombomere of the hindbrain. In tadpole stage embryos, expression was furthermore observed in periventricular regions of the developing brain and the rostral part of the spinal cord.  (+info)

Intact aggrecan and fragments generated by both aggrecanse and metalloproteinase-like activities are present in the developing and adult rat spinal cord and their relative abundance is altered by injury. (8/43)

Aggrecan is a large proteoglycan (PG) that has been grouped with different PG families on the basis of its physical characteristics. These families include the chondroitin sulfate PGs, which appear to inhibit the migration of cells and axons during development. Although aggrecan has been studied primarily in cartilage, in the present study, tissue samples from developing, mature, and injured-adult rat spinal cords were used to determine whether aggrecan is present in the mammalian spinal cord. By the use of Western blot analysis, tissues were probed with aggrecan-specific antibodies (ATEGQV, TYKHRL, and LEC-7) and aggrecan-specific neoepitope antibodies (NITEGE, FVDIPEN, and TFKEEE) to identify full-length aggrecan and several fragments. Unlike many other aggrecan gene family members, aggrecan species were similar in embryonic day 14, postnatal day 1, and adult spinal cords. Spinal cord injury caused significant decreases in aggrecan. Partial recovery in some aggrecan species was evident by 2 weeks after injury. The presence of specific aggrecan neoepitopes suggested that aggrecan is cleaved in the spinal cord by both a disintegrin and metalloproteinase thrombospondin (also known as aggrecanase) and metalloproteinase-like activities. Many aggrecan species found in the spinal cord were similar to species in cartilage. Additional antibodies were used to identify two other aggrecan gene family members, neurocan and brevican, in the adult spinal cord. These studies present novel information on the aggrecan core protein species and enzymes involved in aggrecan cleavage in vivo in the rat spinal cord throughout development and after injury. They also provide the basis for investigating the function of aggrecan in the spinal cord.  (+info)

We show here that, within the brain, invasive ability can be conferred on a noninvasive glioma cell line by expression of an N-terminal fragment of the BEHAB/brevican protein but not by expression of the full-length protein. Because partial proteolytic cleavage of the BEHAB/brevican protein occurs endogenously in both human and rodent invasive brain tumors, these results provide an explanation for glioma cell motility in the adult human brain. In addition, they suggest new therapeutic possibilities for human brain tumor.. Many studies have suggested a central role for extracellular matrix proteins in tumor growth and motility (Paulus et al., 1996; Haugland et al., 1997; Merzak and Pilkington, 1997). HA and HA-binding proteins have been implicated in tumor metastasis (Knudson and Knudson, 1993;Entwistle et al., 1996). For example, the HA-binding protein RHAMM increases the metastatic potential of fibroblast cell lines (Hall et al., 1995). Our present results provide evidence that expression of ...
A disintegrin and metalloproteinase with thrombospondin motifs 4 is an enzyme that in humans is encoded by the ADAMTS4 gene.This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene lacks a C-terminal TS motif. It can degrade aggrecan, a major proteoglycan of cartilage, brevican, a brain-specific extracellular matrix protein, neurocan and versican. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma ...
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모체의 혈청 내 호모시스테인 수준은 태아 성장에 중요한 요인으로 부각되고 있으며 영아의 정상적인 발달과 성장을 위하여 비타민 B₂, 비타민 B_(6), 엽산, 비타민 B_(12) 영양상태가 중요한 것으로 알려져 왔다. 본 연구에서는 생후 12개월 영아의 성장에 관련성이 있을 것이라 사료되는 모체의 임신 중반기의 혈청 호모시스테인 수치와 혈청 비타민 B군 수준, 영아의 비타민 B군 섭취상태에 따른 영아의 성장 발달을 살펴보았다. 또한 영아의 수유상황과 보충제 복용유무, 질병실태에 따른 영아의 영양섭취상태와 성장을 살펴보았다. 이 연구는 전향적 코호트 연구로서 기존에 E 대학 병원 산부인과에서 임신 24-28주의 임산부 308명을 대상으로 이루어졌다. 이들에게서 태어난 영아들 중 생후 1년째의 영아 110명을 대상으로 신체 계측을 실시하고, 식이섭취조사와 ...
Hyaluronan and proteoglycan link protein 2 (HAPLN2) also known as brain link protein 1 (BRAL1) is a protein that in humans is encoded by the HAPLN2 gene. HAPLN1 codes for a related link protein that is expressed in cartilage while Bral1 is expressed in brain. Bral1 interacts with versican and brevican in nodes of Ranvier. In mice with reduced Bralp1 expression the extracellular matrix at nodes of Ranvier is disrupted and action potential conduction is abnormal. GRCh38: Ensembl release 89: ENSG00000132702 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000004894 - Ensembl, May 2017 Human PubMed Reference:. Mouse PubMed Reference:. Entrez Gene: hyaluronan and proteoglycan link protein 2. Bekku Y, Vargová L, Goto Y, Vorísek I, Dmytrenko L, Narasaki M, Ohtsuka A, Fässler R, Ninomiya Y, Syková E, Oohashi T (Feb 2010). Bral1: its role in diffusion barrier formation and conduction velocity in the CNS. The Journal of Neuroscience. 30 (8): 3113-23. doi:10.1523/JNEUROSCI.5598-09.2010. ...
Continent catheterizable reconstruction: One of a group of internal reservoirs or new bladders (neobladders) that are not attached to the urethra. Instead, it is emptied through catheterization, usually through a special attachment to the skin that is similar to but smaller than the stoma for an ileal conduit. The continent reservoir is formed from a piece of small intestine to hold urine after the bladder has been removed. Continent reconstructions are often referred to by names given to them at the institution where the particular type of reconstruction was developed, examples are Indiana Pouch and Continent Cutaneous Pouch ...
Blog on HAPLN1 cdna clone product: The HAPLN1 hapln1 (Catalog #MBS1270699) is a cDNA Clone and is intended for research purposes only. The p...
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Comparative studies on the distribution of hyaluronidase-sensitive ECM glycosaminoglycans in wild-type and reeler mice suggest a role for HA-associated cues in the formation of neocortical and hippocampal lamination (Derer and Nakanishi, 1983; Nakanishi, 1983). A subset of HA-associated proteoglycans, the chondroitinsulfate (CS) proteoglycans (CSPGs), are distributed with layer specificity in the developing cortex and are colocalized with growing fiber tracts (Pearlman and Sheppard, 1996). Analysis of the distribution of ECM components in the reeler mouse brain indicates an important function of CSPGs in the formation of cortical lamination (Sheppard and Pearlman, 1997). CSPGs were also shown to be involved in cell-cell adhesion and cell substrate adhesion during development (Hernon and Lander, 1990; Faissner and Steindler, 1995; Margolis and Margolis, 1997; Rauch, 1997; Yamada et al., 1997). A number of HA-binding proteoglycans, including aggrecan, versican, neurocan and brevican have been ...
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As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Buy our Recombinant Human HAPLN1 protein (denatured). Ab180280 is a full length protein produced in Escherichia coli and has been validated in SDS-PAGE. Abcam…
经过群里指导,换了一张250g(双任务A+B)的机械进行测试,发现速度能跑上去了。经过我的研究,发现跑的流量基本全是B任务的 ...
经过群里指导,换了一张250g(双任务A+B)的机械进行测试,发现速度能跑上去了。经过我的研究,发现跑的流量基本全是B任务的 ...
The CD44 antigens are transmembrane glycoproteins and members of the hyaladherin family of hyaluronan-binding proteins. Multiple CD44 isoforms have been described, the predominant form being CD44S, a glycoprotein of 85 kDa. CD44 is present on most cells or tissues, but not on platelets, hepatocytes, cardiac muscle, kidney tubular epithelium, testis and skin portions. The human blood group antigens Ina/b reside on CD44. *Alexa Fluor and Pacific Blue are registered trademarks of Molecular Probes, Inc.
Monoclonal antibody against neurocan receptor expressed by for use in Immunohistochemistry, Immunoprecipitation, Western Blot against Chicken, Human, Mouse, Rat
Complete information for HAPLN3 gene (Protein Coding), Hyaluronan And Proteoglycan Link Protein 3, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Perineuronal nets (PNNs) are specialized substructures of the neural extracellular matrix (ECM) which envelop the cell soma and proximal neurites of particular sets of neurons with apertures at sites of synaptic contact. Previous studies have shown that PNNs are enriched with chondroitin sulfate proteoglycans (CSPGs) and hyaluronan, however, a complete understanding of their precise molecular composition has been elusive. In addition, identifying which specific PNN components are critical to the formation of this structure has not been demonstrated. Previous work in our laboratory has demonstrated that the CSPG, aggrecan, is a key activity-dependent component of PNNs in vivo. In order to assess the contribution of aggrecan to PNN formation, we utilized cartilage matrix deficiency (cmd) mice, which lack aggrecan. Herein, we utilized an in vitro model, dissociated cortical culture, and an ex vivo model, organotypic slice culture, to specifically investigate the role aggrecan plays in PNN ...
Alterations in the structure and physiology of interneurons in the prefrontal cortex (PFC) are important factors in the etiopathology of different psychiatric disorders. Among the interneuronal subpopulations, parvalbumin (PV) expressing cells appear to be specially affected. Interestingly, during development and adulthood the connectivity of these interneurons is regulated by the presence of perineuronal nets (PNNs), specialized regions of the extracellular matrix, which are frequently surrounding PV expressing neurons. Previous reports have found anomalies in the density of PNNs in the PFC of schizophrenic patients. However, although some studies have described alterations in PNNs in some extracortical regions of bipolar disorder patients, there are no studies focusing on the prefrontocortical PNNs of bipolar or major depression patients. For this reason, we have analyzed the density of PNNs in post-mortem sections of the dorsolateral PFC (DLPFC) from the Stanley Neuropathology Consortium, which
Glioblastoma multiforme (GBM) are highly infiltrative, aggressive and lethal primary brain tumors in adults that are resistant to conventional treatments. A major clinical obstacle in the treatment of GBMs is the ability of glioma cells to invade the surrounding brain parenchyma, preventing their complete surgical resection. Several studies have shown that invasive glioma stem cells (GSCs) extend beyond the surgically resected regions. These invasive cells are the cause for tumor recurrence and poor prognosis in GBM patients. The molecular and cellular mechanism involved in the regulation of the switch from proliferation to invasion/migration in GBM is poorly understood. We investigated the role of Olig2, a Central Nervous System (CNS) specific transcription factor, in promoting the switch from proliferating to invading glioma cell. Genetically relevant murine glioma model as well as patient-derived glioma stem cells (GSCs) were utilized to identify the effect of Olig2 on glioma invasion. ...
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TY - JOUR. T1 - Characterization of dermacan, a novel zebrafish lectican gene, expressed in dermal bones. AU - Kang, Jeong Suk. AU - Oohashi, Toshitaka. AU - Kawakami, Yasuhiko. AU - Bekku, Yoko. AU - Izpisúa Belmonte, Juan Carlos. AU - Ninomiya, Yoshifumi. PY - 2004/3. Y1 - 2004/3. N2 - We report here the isolation and characterization of a cDNA encoding zebrafish dermacan, a novel member of hyaluronan (HA)-binding proteoglycans, which was termed after its characteristic expression in the zebrafish dermal bones. The deduced protein sequence shares the typical modular elements of lecticans. Sequence comparison covering the C-terminal globular domain demonstrated that dermacan shows high homology with zebrafish versican but is distinct from any other identified lecticans. Genomic DNA analysis demonstrated that dermacan and versican were encoded by distinct genes in the zebrafish genome. The expression of dermacan is initiated in the sclerotome and cephalic paraxial mesoderm at 16 h ...
Versican, also called PG-M, is a lectican family proteoglycan that is present in the fetal, neonatal, and adult dermis in humans. It is a member of a family of large, aggregating proteoglycans that primarily bear chondroitin sulfate glycosaminoglycan side chains.Versican interacts with both fibrillin-1 and isolated microfibrils. Versican binds fibulin-1 and -2 via its lectin-like domain and localizes to cutaneous microfibrils. Versican also binds to hyaluronic acid (HA) via its N-terminal region and HA is co-distributed with elastic fibers in the dermis. Therefore, by binding to fibrillin microfibrils and HA, versican can impart viscous properties to cutaneous microfibrils. The loss of the HA-binding ability of versican followed by HA exclusion may be responsible for the pathological and phenotypical changes observed in solar elastosis.. Keiko Hasegawa, Masahiko Yoneda, Hiroko Kuwabara, Osamu Miyaishi, Naoki Itano, Akiko Ohno, Masahiro Zako and Zenzo Isogai (2007). Versican, a Major ...
Neurocan core protein is a protein that in humans is encoded by the NCAN gene. Neurocan is a member of the lectican / chondroitin sulfate proteoglycan protein families and consists of neurocan core protein and chondroitin sulfate. It is thought to be involved in the modulation of cell adhesion and migration. Neurocan is a significant component of the extracellular matrix, and its levels are modulated by a variety of factors, but mice in which the NCAN gene has been knocked out show no easily observable defects in brain development or behavior. However, a genome-wide association study published in 2011 identified Neurocan as a susceptibility factor for bipolar disorder. A more comprehensive study published in 2012 confirmed that association. The 2012 study examined correlations between NCAN alleles and various symptoms of bipolar disorder, and also examined the behavior of NCAN knockout mice. In the human subjects, it was found that NCAN genotype was strongly associated with manic symptoms but ...
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Corroborating previous research, the new study, which was published online in Anticancer Research, found that low pre-treatment serum testosterone predicted poor overall, cancer-specific, and progression-free survival after primary ADT. The median serum testosterone level was 397 ng/dL. The median overall, cancer-specific, and progression-free survival rates were 68.1, 68.9, and 23.2 months, respectively. Men with higher testosterone levels in the second to fourth quartiles showed similar survival ...
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The company is looking to expand the label of Cysview to include its use in the outpatient setting to detect the recurrence of bladder cancer using a flexible cystoscope, the detection of carcinoma in situ (CIS) and the repeat administration of Cysview. The filing is a combination drug-device application, with the KARL STORZ D-LIGHT C PDD Flexible Videoscope System.. We are delighted to see the FDA expedite the review for this sNDA as it will offer patients improved surveillance of their Non-Muscle Invasive Bladder Cancer (NMIBC), commented Andrea Maddox-Smith CEO, Bladder Cancer Advocacy Network (BCAN). BCAN is the only national advocacy organization devoted to advancing bladder cancer research and supporting those impacted by the disease.. We look forward to hearing a decision from the FDA early next year on the US Cysview® label expansion to include patients undergoing surveillance cystoscopy using a flexible scope. The sNDA also includes detection of CIS and to allow for repeated use in ...
In the rodent model of temporal lobe epilepsy, there is extensive synaptic reorganization within the hippocampus following a single prolonged seizure event, after which animals eventually develop epilepsy. The perineuronal net (PN), a component of the neural extracellular matrix (ECM), primarily surrounds inhibitory interneurons and, under normal conditions, restricts synaptic reorganization. The objective of the current study was to explore the effects of status epilepticus (SE) on PNs in the adult hippocampus. The aggrecan component of the PN was studied, acutely (48 h post-SE), sub-acutely (1 week post-SE) and during the chronic period (2 months post-SE). Aggrecan expressing PNs decreased by 1 week, likely contributing to a permissive environment for neuronal reorganization, and remained attenuated at 2 months. The SE-exposed hippocampus showed many PNs with poor structural integrity, a condition rarely seen in controls. Additionally, the decrease in the aggrecan component of the PN was ...
MECHANISM OF HYALURONAN BINDING AND DEGRADATION: STRUCTURE OF STREPTOCOCCUS PNEUMONIAE HYALURONATE LYASE IN COMPLEX WITH HYALURONIC ACID DISACCHARIDE AT 1.7 A ...
OPC bold driver CC grad. desc. CC bold driver ECOC BCH grad. desc. ECOC BCH bold driver (a) (b) Figure 1: Comparison of performances of different decomposition methods on glass (a) and optdigits (b) data sets of the UCI. Table 4: Standard PWC (left) and CC (right) decomposition matrices. 0 B B B B B @ +1 1 0 0 +1 0 1 0 +1 0 0 1 0 +1 1 0 0 +1 0 1 0 0 +1 1 1 C C C C C A 0 B B B B B @ +1 +1 1 1 +1 1 +1 ...
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This work is supported by NSF grant nos. IIS-0612049, CNS-0916908 and CNS-0615277, a grant from the SRC under task no. 1607, and grants from NVIDIA Research and NEC labs. ...
This work is supported by NSF grant nos. IIS-0612049, CNS-0916908 and CNS-0615277, a grant from the SRC under task no. 1607, and grants from NVIDIA Research and NEC labs. ...
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Project Description: Grade IV astrocytomas known as glioblastoma multiforme (GBM) are the most aggressive primary brain tumors, with a median survival rate of 15 months after diagnosis. Current methodologies for diagnosis include time-consuming histopathological reviews of biopsied brain tumor tissue to determine malignancy, which can delay early diagnosis and treatment. We propose to use microfluidics in combination with labeled magnetic beads to separate invasive glioma cells from other healthy neural tissue within a few hours. A strategy that combines rapid cell sorting with subsequent cell culture of a pure population of the patients glioma cells could not only speed up time to diagnosis, but also enhance personalized treatment. We hypothesize that incubating magnetic beads labeled with anti-epithelial cell adhesion molecule (epCAM) antibody will allow for the selective separation of glioma cells from other neural tissue cell types from co-culture, as judged by recent evidence that glioma ...
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The peptidoglycan monomers are synthesized in the cytosol and are then attached to a membrane carrier bactoprenol. Bactoprenol transports peptidoglycan monomers across the cell membrane where they are inserted into the existing peptidoglycan.[6] In the first step of peptidoglycan synthesis, glutamine, which is an amino acid, donates an amino group to a sugar, fructose 6-phosphate. This turns fructose 6-phosphate into glucosamine-6-phosphate. In step two, an acetyl group is transferred from acetyl CoA to the amino group on the glucosamine-6-phosphate creating N-acetyl-glucosamine-6-phosphate.[7] In step three of the synthesis process, the N-acetyl-glucosamine-6-phosphate is isomerized, which will change N-acetyl-glucosamine-6-phosphate to N-acetyl-glucosamine-1-phosphate.[7] In step 4, the N-acetyl-glucosamine-1-phosphate, which is now a monophosphate, attacks UTP. Uridine triphosphate, which is a pyrimidine nucleotide, has the ability to act as an energy source. In this particular reaction, ...
... s (/ˈmjuːsɪn/) are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by epithelial tissues in most animals.[1] Mucins' key characteristic is their ability to form gels; therefore they are a key component in most gel-like secretions, serving functions from lubrication to cell signalling to forming chemical barriers.[1] They often take an inhibitory role.[1] Some mucins are associated with controlling mineralization, including nacre formation in mollusks,[2] calcification in echinoderms[3] and bone formation in vertebrates.[4] They bind to pathogens as part of the immune system. Overexpression of the mucin proteins, especially MUC1, is associated with many types of cancer.[5] Although some mucins are membrane-bound due to the presence of a hydrophobic membrane-spanning domain that favors retention in the plasma membrane, most mucins are secreted as principal components of mucus by mucous membranes or are secreted to become a component of saliva. ...
brevican, 145kDa keratan sulfate fibromodulin, 42 kDa. lumican, 38 kDa Certain members are considered members of the "small ...
... (ORM) or alpha-1-acid glycoprotein (α1AGp,[1] AGP or AAG) is an acute phase (acute phase protein) plasma alpha-globulin glycoprotein and is modulated by two polymorphic genes. It is synthesized primarily in hepatocytes and has a normal plasma concentration between 0.6-1.2 mg/mL (1-3% plasma protein).[2] Plasma levels are affected by pregnancy, burns, certain drugs, and certain diseases, particularly HIV.[2] The only established function of ORM is to act as a carrier of basic and neutrally charged lipophilic compounds. In medicine, it is known as the primary carrier of basic (positively charged) drugs (whereas albumin carries acidic (negatively charged) and neutral drugs), steroids, and protease inhibitors.[2][3] Aging causes a small decrease in plasma albumin levels; if anything, there is a small increase in alpha-1-acid glycoprotein. The effect of these changes on drug protein binding and drug delivery, however, appear to be minimal.[4] AGP shows a complex interaction with thyroid ...
... s are proteins which contain oligosaccharide chains (glycans) covalently attached to amino acid side-chains. The carbohydrate is attached to the protein in a cotranslational or posttranslational modification. This process is known as glycosylation. Secreted extracellular proteins are often glycosylated. Carbohydrates are attached to some proteins to form glycoproteins. In proteins that have segments extending extracellularly, the extracellular segments are also often glycosylated. Glycoproteins are also often important integral membrane proteins, where they play a role in cell-cell interactions. It is important to distinguish endoplasmic reticulum-based glycosylation of the secretory system from reversible cytosolic-nuclear glycosylation. Glycoproteins of the cytosol and nucleus can be modified through the reversible addition of a single GlcNAc residue that is considered reciprocal to phosphorylation and the functions of these are likely to be additional regulatory mechanism that ...
4-1BB is a type 2 transmembrane glycoprotein receptor belonging to the TNF superfamily, expressed on activated T Lymphocytes.[1] 4-1BBL (4-1BB ligand) is found on APCs (antigen presenting cells) and binds to 4-1BB. ...
... is a multistructural and multifunctional cell surface molecule involved in cell proliferation, cell differentiation, cell migration, angiogenesis, presentation of cytokines, chemokines, and growth factors to the corresponding receptors, and docking of proteases at the cell membrane, as well as in signaling for cell survival. All these biological properties are essential to the physiological activities of normal cells, but they are also associated with the pathologic activities of cancer cells. Experiments in animals have shown that targeting of CD44 by antibodies, antisense oligonucleotides, and CD44-soluble proteins markedly reduces the malignant activities of various neoplasms, stressing the therapeutic potential of anti-CD44 agents. High levels of the adhesion molecule CD44 on leukemic cells are essential to generate leukemia.[24] Furthermore, because alternative splicing and posttranslational modifications generate many different CD44 sequences, including, perhaps, tumor-specific ...
Talk:Brevican. *Talk:Bridge locus. *Talk:British Neuroscience Association. *Talk:Brodmann area 12 ...
بريفيكان‏ (Brevican) هوَ بروتين يُشَفر بواسطة جين بريفيكان في الإنسان.[1][2][3][4][5] ... "Entrez Gene: BCAN brevican". مؤرشف من الأصل في 05 ديسمبر 2010.. الوسيط ,CitationClass=. تم تجاهله (مساعدة). ... BCAN, BEHAB, CSPG7, Brevican. معرفات خارجية. الوراثة المندلية البشرية عبر الإنترنت 600347 MGI: MGI:1096385 HomoloGene: 7244 ... Dong Y, Han X, Xue Y, Dong B, Guo X, Hu G, Zhu C, Lu Y (Feb 2004). "Secreted brevican mRNA is expressed in the adult rat ...
Tangye SG، Nichols KE، Hare NJ، van de Weerdt BC (2003). "Functional requirements for interactions between CD84 and Src homology 2 domain-containing proteins and their contribution to human T cell activation". J. Immunol. 171 (5): 2485-95. PMID 12928397. doi:10.4049/jimmunol.171.5.2485. ...
Vanhalst K، Kools P، Vanden Eynde E، van Roy F (2001). "The human and murine protocadherin-beta one-exon gene families show high evolutionary conservation, despite the difference in gene number". FEBS Lett. 495 (1-2): 120-5. PMID 11322959. doi:10.1016/S0014-5793(01)02372-9. ...
Schäfer BW، Wicki R، Engelkamp D، Mattei MG، Heizmann CW (1995). "Isolation of a YAC clone covering a cluster of nine S100 genes on human chromosome 1q21: rationale for a new nomenclature of the S100 calcium-binding protein family". Genomics. 25 (3): 638-43. PMID 7759097. doi:10.1016/0888-7543(95)80005-7. ...
Nagase T، Ishikawa K، Kikuno R، وآخرون. (2000). "Prediction of the coding sequences of unidentified human genes. XV. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.". DNA Res. 6 (5): 337-45. PMID 10574462. doi:10.1093/dnares/6.5.337. الوسيط ...
Page AB، Owen CR، Kumar R، Miller JM، Rafols JA، White BC، DeGracia DJ، Krause GS (Jul 2003). "Persistent eIF2alpha(P) is colocalized with cytoplasmic cytochrome c in vulnerable hippocampal neurons after 4 hours of reperfusion following 10-minute complete brain ischemia". Acta Neuropathologica. 106 (1): 8-16. PMID 12687390. doi:10.1007/s00401-003-0693-2. الوسيط ...
de Graaf K، Hekerman P، Spelten O، وآخرون. (2004). "Characterization of cyclin L2, a novel cyclin with an arginine/serine-rich domain: phosphorylation by DYRK1A and colocalization with splicing factors". J. Biol. Chem. 279 (6): 4612-24. PMID 14623875. doi:10.1074/jbc.M310794200. ...
Neely GG، Hess A، Costigan M، Keene AC، Goulas S، Langeslag M، Griffin RS، Belfer I، Dai F، Smith SB، Diatchenko L، Gupta V، Xia CP، Amann S، Kreitz S، Heindl-Erdmann C، Wolz S، Ly CV، Arora S، Sarangi R، Dan D، Novatchkova M، Rosenzweig M، Gibson DG، Truong D، Schramek D، Zoranovic T، Cronin SJ، Angjeli B، Brune K، Dietzl G، Maixner W، Meixner A، Thomas W، Pospisilik JA، Alenius M، Kress M، Subramaniam S، Garrity PA، Bellen HJ، Woolf CJ، Penninger JM (2010). "A genome-wide Drosophila screen for heat nociception identifies α2δ3 as an evolutionarily conserved pain gene". Cell. 143 (4): 628-38. PMC 3040441 ...
Robinson NA، Lapic S، Welter JF، Eckert RL (1997). "S100A11, S100A10, annexin I, desmosomal proteins, small proline-rich proteins, plasminogen activator inhibitor-2, and involucrin are components of the cornified envelope of cultured human epidermal keratinocytes.". J. Biol. Chem. 272 (18): 12035-46. PMID 9115270. doi:10.1074/jbc.272.18.12035. ...
Fukuda A، Tokonabe S، Hamada M، Matsumoto M، Tsukui T، Nogi Y، Hisatake K (April 2003). "Alleviation of PC4-mediated transcriptional repression by the ERCC3 helicase activity of general transcription factor TFIIH". The Journal of Biological Chemistry. 278 (17): 14827-31. PMID 12590132. doi:10.1074/jbc.M213172200. ...
Kumanogoh A، Marukawa S، Suzuki K، Takegahara N، Watanabe C، Ch'ng E، Ishida I، Fujimura H، Sakoda S، Yoshida K، Kikutani H (2002). "Class IV semaphorin Sema4A enhances T-cell activation and interacts with Tim-2.". Nature. 419 (6907): 629-33. PMID 12374982. doi:10.1038/nature01037. ...
Ciosk R، Shirayama M، Shevchenko A، Tanaka T، Toth A، Shevchenko A، Nasmyth K (2000). "Cohesin's binding to chromosomes depends on a separate complex consisting of Scc2 and Scc4 proteins". Molecular Cell. 5 (2): 243-54. PMID 10882066. doi:10.1016/S1097-2765(00)80420-7. .mw-parser-output cite.citation{font-style:inherit}.mw-parser-output .citation q{quotes:"\"""\"""'""'"}.mw-parser-output .citation .cs1-lock-free a{background:url("//upload.wikimedia.org/wikipedia/commons/thumb/6/65/Lock-green.svg/9px-Lock-green.svg.png")no-repeat;background-position:right .1em center}.mw-parser-output .citation .cs1-lock-limited a,.mw-parser-output .citation .cs1-lock-registration a{background:url("//upload.wikimedia.org/wikipedia/commons/thumb/d/d6/Lock-gray-alt-2.svg/9px-Lock-gray-alt-2.svg.png")no-repeat;background-position:right .1em center}.mw-parser-output .citation .cs1-lock-subscription ...
Dias Neto E, Correa RG, Verjovski-Almeida S, Briones MR, Nagai MA, da Silva W, Zago MA, Bordin S, Costa FF, Goldman GH, Carvalho AF, Matsukuma A, Baia GS, Simpson DH, Brunstein A, de Oliveira PS, Bucher P, Jongeneel CV, O'Hare MJ, Soares F, Brentani RR, Reis LF, de Souza SJ, Simpson AJ (2000). "Shotgun sequencing of the human transcriptome with ORF expressed sequence tags". Proc. Natl. Acad. Sci. U.S.A. 97 (7): 3491-6. doi:10.1073/pnas.97.7.3491. PMC 16267. PMID 10737800 ...
Pratama A، Ramiscal RR، Silva DG، Das SK، Athanasopoulos V، Fitch J، Botelho NK، Chang PP، Hu X، Hogan JJ، Maña P، Bernal D، Korner H، Yu D، Goodnow CC، Cook MC، Vinuesa CG (April 2013). "Roquin-2 shares functions with its paralog Roquin-1 in the repression of mRNAs controlling T follicular helper cells and systemic inflammation". Immunity. 38 (4): 669-80. PMID 23583642. doi:10.1016/j.immuni.2013.01.011. ...
Colomer V، Engelender S، Sharp AH، Duan K، Cooper JK، Lanahan A، Lyford G، Worley P، Ross CA (September 1997). "Huntingtin-associated protein 1 (HAP1) binds to a Trio-like polypeptide, with a rac1 guanine nucleotide exchange factor domain". Hum. Mol. Genet. 6 (9): 1519-25. PMID 9285789. doi:10.1093/hmg/6.9.1519. .mw-parser-output cite.citation{font-style:inherit}.mw-parser-output .citation q{quotes:"\"""\"""'""'"}.mw-parser-output .citation .cs1-lock-free a{background:url("//upload.wikimedia.org/wikipedia/commons/thumb/6/65/Lock-green.svg/9px-Lock-green.svg.png")no-repeat;background-position:right .1em center}.mw-parser-output .citation .cs1-lock-limited a,.mw-parser-output .citation .cs1-lock-registration a{background:url("//upload.wikimedia.org/wikipedia/commons/thumb/d/d6/Lock-gray-alt-2.svg/9px-Lock-gray-alt-2.svg.png")no-repeat;background-position:right .1em center}.mw-parser-output .citation .cs1-lock-subscription ...
Matsushita M; Yamadori T; Kato S; Takemoto Y; Inazawa J; Baba Y; Hashimoto S; Sekine S; Arai S; Kunikata T; Kurimoto M; Kishimoto T; Tsukada S (June 1998). "Identification and characterization of a novel SH3-domain binding protein, Sab, which preferentially associates with Bruton's tyrosine kinase (BtK)". Biochem Biophys Res Commun. 245 (2): 337-43. doi:10.1006/bbrc.1998.8420. PMID 9571151 ...
Mori K، Kato H (2002). "A putative nuclear receptor coactivator (TMF/ARA160) associates with hbrm/hSNF2 alpha and BRG-1/hSNF2 beta and localizes in the Golgi apparatus". FEBS Lett. 520 (1-3): 127-32. PMID 12044884. doi:10.1016/S0014-5793(02)02803-X. ...
Ishikawa K، Nagase T، Suyama M، وآخرون. (1998). "Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro.". DNA Res. 5 (3): 169-76. PMID 9734811. doi:10.1093/dnares/5.3.169. ...
Lucas FR، Goold RG، Gordon-Weeks PR، Salinas PC (1998). "Inhibition of GSK-3beta leading to the loss of phosphorylated MAP-1B is an early event in axonal remodelling induced by WNT-7a or lithium". J. Cell Sci. 111 ( Pt 10) (10): 1351-61. PMID 9570753. ...
Loyet KM، Kowalchyk JA، Chaudhary A، وآخرون. (1998). "Specific binding of phosphatidylinositol 4,5-bisphosphate to calcium-dependent activator protein for secretion (CAPS), a potential phosphoinositide effector protein for regulated exocytosis.". J. Biol. Chem. 273 (14): 8337-43. PMID 9525942. doi:10.1074/jbc.273.14.8337. الوسيط ...
Nagase T، Seki N، Ishikawa K، وآخرون. (1997). "Prediction of the coding sequences of unidentified human genes. VI. The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain". DNA Res. 3 (5): 321-9, 341-54. PMID 9039502. doi:10.1093/dnares/3.5.321. ...
Nagase T، Ishikawa K، Nakajima D، Ohira M، Seki N، Miyajima N، Tanaka A، Kotani H، Nomura N، Ohara O (Apr 1997). "Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Research. 4 (2): 141-50. PMID 9205841. doi:10.1093/dnares/4.2.141. ...
In 9L-BEHAB/brevican tumors (lane 1), immunoreactivity for BEHAB/brevican is at 140 kDa. No evidence of a cleavage product is ... One set of transfectants, 9L-BEHAB/brevican, carried a construct encoding full-length BEHAB/brevican (amino acids 1-883), and a ... To study the protein product of the BEHAB/brevican mRNA, we examined BEHAB/brevican protein expression in experimental rodent ... BEHAB/brevican mRNA is detected. By contrast, BEHAB/brevican is undetectable in tumors that are not of glial origin, including ...
Brevican core protein, Brain-enriched hyaluronan-binding protein, BEHAB, Chondroitin sulfate proteoglycan 7, BCAN, BEHAB, CSPG7 ...
The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system ... It can degrade aggrecan, a major proteoglycan of cartilage, brevican, a brain-specific extracellular matrix protein, neurocan ...
Gavin Wrights lab contains the insert Brevican mouse monoclonal antibody and is published in BMC Biol. 2010 . 8():76. This ... SI10-Brevican was a gift from Gavin Wright (Addgene plasmid # 46300 ; http://n2t.net/addgene:46300 ; RRID:Addgene_46300) ...
Brevican: A BRAIN-specific hyalectin that may play a role in terminally differentiating NEURONS. It is found highly ... Brevican. Subscribe to New Research on Brevican A BRAIN-specific hyalectin that may play a role in terminally differentiating ... 01/01/2012 - "The aim of this study was to examine whether brevican is a predictor of glioma and its roles in glioma cell ... 01/01/2012 - "Moreover, the role of brevican in the growth and progression of glioma was demonstrated by in vivo studies. ". 01 ...
Compare and order Brevican ELISA Kits. View citations, images, detection ranges, sensitivity, prices and more. Recommended ... brevican core protein , brevican , brevican core protein-like , brevican soluble core protein , Brevican core protein , brain- ... Search Brevican ELISA Kits for other reactivities: Guinea Pig,. Pig (Porcine),. Dog (Canine),. Goat,. Cow (Bovine),. Chicken,. ... Images for product: Brevican (BCAN) ELISA Kit Diagramm of the ELISA kit to detect Human BCANwith the optical density on the x- ...
In contrast to the other family members, brevican occurs both as soluble isoforms secreted into the extracellular space and ... Brevican is a member of the aggrecan/versican family of proteoglycans. ... Brevican is a member of the aggrecan/versican family of proteoglycans. In contrast to the other family members, brevican occurs ... sensitive brevican immunoreactivity. During ontogenetic development, both brevican transcripts are generally up-regulated. ...
Exogenously added brevican binds to the surfaces of rat neural cell lines. A, Exogenously added brevican and brevican core ... Brevican was first cloned from adult bovine brain (Yamada et al., 1994). Since then, rat brevican (Yamada et al., 1995) and a ... Because the brevican core protein contains a hyaluronan-binding domain at its N terminal, it is conceivable that brevican binds ... 9C). The inhibitory effect of brevican was also apparent in a substrate prepared from 50 μg/ml brevican and 50 μg/ml laminin, ...
Brevican It is a Custom assay which can detect BCAN / Brevican down to 0.313 ng/ml. ... Brevican ELISA Kit LS-F41799 is a 96-Well enzyme-linked immunosorbent assay for the Quantitative detection of Porcine BCAN / ... It is based upon a Custom assay principle and can be used to detect levels of BCAN / Brevican as low as 0.188 nanograms per ... LS-F41799 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Porcine BCAN / Brevican. ...
BEHAB/brevican has been cloned from bovine, mouse and rat. Two isoforms have been reported: a f … ... brevican, a brain-specific member of the lectican family of chondroitin sulfate proteoglycans (CSPGs), may play a role in both ... First, BEHAB/brevican maps to human chromosome 1q31. Second, we report the sequence of both isoforms of human BEHAB/brevican. ... BEHAB/brevican has been cloned from bovine, mouse and rat. Two isoforms have been reported: a full-length isoform that is ...
Fusion protein amino acids 219-655 of rat Brevican (also known as Brevican core protein, Brainenriched hyaluronan-binding ... Brevican is involved in the development and formation of the brain extracellular matrix and may be involved in malignancy of ... Brevican core protein, Chondroitin sulfate proteoglycan 7 or Brain-enriched hyaluronan-binding protein is encoded by the gene ... Brevican is developmentally regulated and specifically expressed in the central nervous system. Diseases associated with this ...
Expression of brevican in mouse tooth. Bcan. Species: mouse Location in mouse genome: chromosome 3, 90072619 - 90091338 (UCSC, ...
Selective cleavage of the Glu395-Ser396 bond of brevican, one of the major proteoglycans in adult brain tissues, is thought to ... keywords = "ADAMTS, Brevican, Digestion, Glioma, Invasion",. author = "Mitsutoshi Nakada and Hisashi Miyamori and Daisuke Kita ... N2 - Selective cleavage of the Glu395-Ser396 bond of brevican, one of the major proteoglycans in adult brain tissues, is ... AB - Selective cleavage of the Glu395-Ser396 bond of brevican, one of the major proteoglycans in adult brain tissues, is ...
IMAGE: Brevican (in green) forms a meshwork in hippocampus. In this image, Brevican is expressed by two Parvalbumin cells (in ... In this new study, the researchers found that one of these proteins called Brevican, which is also one of the most abundant ... These novel findings show that Brevican is dynamically regulated by experiences coming from the environment and is ... found that this adaptability is shaped by a specific protein called Brevican. Moreover, loss of this protein leads to deficits ...
In the brevican-EGFP fusion construct, EGFP was fused to the C terminus of the full-length cDNA of rat brevican. We introduced ... The brevican-based ECM interacts with NF-186. To determine if NF-186 and/or NrCAM interact with brevican, we expressed each CAM ... Brevican interacts with NF-186. (A-D) Soluble GFP-brevican (green) binds to COS-7 cells transfected with HA-tagged NF-186 (A [ ... The view that brevican is a ligand for NF-186 is consistent with the fact that brevican binds strongly to fibronectin type III ...
Brevican. A BRAIN-specific hyalectin that may play a role in terminally differentiating NEURONS. It is found highly ...
Brevican. A BRAIN-specific hyalectin that may play a role in terminally differentiating NEURONS. It is found highly ...
Neurocan and brevican are expressed predominantly in brain tissue. Versican and aggrecan are anchored to hyaluronan in tissues ... brevican, and aggrecan), and each contains a contiguous homologous link molecule (Figure 15.3). Versican is a major component ... by similar mechanisms, and it is likely that neurocan and brevican are organized similarly. Thus, hyaluronan acts as a scaffold ...
DE RecName: Full=Brevican core protein; DE Flags: Precursor; GN Name=Bcan; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; ... FT SIGNAL 1 22 {ECO:0000255}. FT CHAIN 23 883 Brevican core protein. FT /FTId=PRO_0000017512. FT DOMAIN 35 154 Ig-like V-type. ... "Sequence and chromosomal localization of the mouse brevican gene."; RL Genomics 44:15-21(1997). RN [2] RP NUCLEOTIDE SEQUENCE [ ... CC {ECO:0000305}. CC -!- WEB RESOURCE: Name=Functional Glycomics Gateway - Glycan Binding; CC Note=Brevican; CC URL="http://www ...
Brevican. NM_021948. NM_198427. Gene Info. BCAP31. B-cell receptor-associated protein 31. NM_001139457. NM_001256447. NM_005745 ...
BEHAB/brevican requires ADAMTS-mediated proteolytic leavage to promote glioma invasion. J Neurooncol. 2008;88:261-72. ...
Brevican (BCAN) production by scar-forming astrocytes and non-astrocyte cells. Images show individual fluorescence channels of ...
Human glioblastomas overexpress ADAMTS-5 that degrades brevican. Acta Neuropathol. (2005) 110:239-46. doi: 10.1007/s00401-005- ... In fact, the presence of both enzymes increases the invasive potential of glioblastoma cancer cells through brevican ...
Brevican knockdown reduces late-stage glioma tumor aggressiveness. . J Neurooncol. 120(1):63-72 (2014). ...
The brain chondroitin sulfate proteoglycan brevican associates with astrocytes ensheathing cerebellar glomeruli and inhibits ...
Brevican. N294A/6. Neuroligin-1. N97A/31. NrCAM. N343/26. SynCAM4. N244/5. ...
2012) Brevican: A key proteoglycan in the perisynaptic extracellular matrix of the brain. Int J Biochem Cell Biol 44(7):1051- ... Hyaluronan, secreted by membrane-bound HA synthase (HAS), binds to members of the lectican family (aggrecan, brevican, versican ...
BCAN (brevican). Need_an_author 2013-05. -. 54150. BCAP29. 7q22.3. 7. BCAP29 (B cell receptor associated protein 29). Need_an_ ...
21) Brevican (BCAN, BEHAB, Genbank accession no. AF229053). Gary S. C., et al Gene 256, 139-147, 2000; Clark H. F., et al ... Brevican; (22) EphB2R; (23) ASLG659; (24) PSCA; (25) GEDA; (26) BAFF-R (B cell-activating factor receptor, BLyS receptor 3, BR3 ...
Seidenbecher, C. I., Richter, K., Rauch, U., Fassler, R., Garner, C. C., and Gundelfinger, E. D. (1995). Brevican, a ... and the extracellular matrix protein Brevican (Seidenbecher et al., 1995). In the following years, interaction studies revealed ...
Brevican is secreted from astrocytes and neurons as a 145 kD core protein that bears up to three, covalently-linked, CS chains ... Brevican is the most abundant chondroitin sulfate proteoglycan in the extracellular matrix of the adult brain. It is a member ...
  • This function is typical of the large aggregating proteoglycans: aggrecan, versican , brevican, and neurocan. (bionity.com)
  • Aggrecan is a member of large, aggregating proteoglycans (also including, versican, brevican and neurocan) found in articular cartilage. (novusbio.com)
  • ADAMTS5 is an extracellular matrix (ECM) degrading enzyme that show proteolytic activity toward the hyalectan group of chondroitin sulfate proteoglycans (CSPGs) including aggrecan, versican, brevican and neurocan. (uniprot.org)
  • Jones LL, Margolis RU, Tustynski MH (2003) The chondroitin sulfate proteoglycans neurocan, brevican, phosphacan, and versican are differenzially regulated following spinal cord injury. (springer.com)
  • In cooperation with Prof. Uwe Rauch from Lund University in Sweden, Bochum's biologists examined cells from the brains of two mouse species: a species with a normal extracellular matrix and a species which lacked four components of the extracellular matrix due to genetic manipulation, namely the molecules tenascin-C, tenascin-R, neurocan and brevican. (bio-medicine.org)
  • Brevican, neurocan, tenascin-C and versican are mainly responsible for the invasiveness of low-grade astrocytoma , PATHOLOGY AND ONCOLOGY RESEARCH 18: (2) pp. 413-420. (doktori.hu)
  • Neurofascin isoform 186 from mouse brain was shown to carry O-mannosyl glycans as well as the lecticans brevican, neurocan and versican from murine and bovine brain. (uni-koeln.de)
  • The degradation activity of ADAMTS-4 was estimated for the core proteins of chondroitin sulfate proteoglycans, that is, brevican, neurocan and phosphacan. (biomedcentral.com)
  • Some extracellular matrix molecules (tenascin-C, DSD- 1 -proteoglycan, neurocan, and brevican) were upregulated within the denervated outer molecular layer after lesion of the entorhinal cortex, suggesting a similar role after lesion. (iospress.com)
  • Brevican is a member of the aggrecan/versican family of proteoglycans. (nih.gov)
  • We also assessed the expression of the ADAMTS substrates aggrecan, brevican, and versican in these neoplasms. (bireme.br)
  • It is a member of matrix metalloproteinase (MMP) family in the ECM which breaks substrates such as aggrecan, versican, brevican, nidogen , and procollagen. (thefreedictionary.com)
  • Brevican is a nervous system-specific chondroitin sulfate proteoglycan that belongs to the aggrecan family and is one of the most abundant chondroitin sulfate proteoglycans in adult brain. (jneurosci.org)
  • BEHAB (Brain Enriched HyAluronan Binding)/brevican, a brain-specific member of the lectican family of chondroitin sulfate proteoglycans (CSPGs), may play a role in both brain development and human glioma. (nih.gov)
  • Brevican core protein, Chondroitin sulfate proteoglycan 7 or Brain-enriched hyaluronan-binding protein is encoded by the gene BCAN and is a member of the lectican family of chondroitin sulfate proteoglycans. (antibodiesinc.com)
  • Selective cleavage of the Glu 395 -Ser 396 bond of brevican, one of the major proteoglycans in adult brain tissues, is thought to be important for glioma cell invasion. (elsevier.com)
  • LS-F41799 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Quantitative detection of Porcine BCAN / Brevican. (lsbio.com)
  • It is based upon a Custom assay principle and can be used to detect levels of BCAN / Brevican as low as 0.188 nanograms per millilter. (lsbio.com)
  • 2012) reported that this disorder in Cavalier King Charles Spaniels is due to a 15.7kb deletion in the BCAN gene, which encodes brain-specific extracellular matrix proteoglycan brevican. (omia.org)
  • neurofascin-186 [NF-186] and neuron glia-related CAM [NrCAM]), cytoskeletal proteins (ankyrinG and βIV spectrin), and the extracellular chondroitin-sulfate proteoglycan brevican. (rupress.org)
  • BEHAB/brevican has been cloned from bovine, mouse and rat. (nih.gov)
  • Here, we report the characterization of BEHAB/brevican isoforms in human brain. (nih.gov)
  • First, BEHAB/brevican maps to human chromosome 1q31. (nih.gov)
  • Second, we report the sequence of both isoforms of human BEHAB/brevican. (nih.gov)
  • The deduced protein sequence of full-length, secreted human BEHAB/brevican is 89.7, 83.3 and 83.2% identical to bovine, mouse and rat homologues, respectively. (nih.gov)
  • Third, by RNase protection analysis (RPA) we show the developmental regulation of BEHAB/brevican isoforms in normal human cortex. (nih.gov)
  • Fourth, we confirm and extend previous studies from our laboratory, here demonstrating the upregulation of BEHAB/brevican mRNA in human glioma quantitatively. (nih.gov)
  • In contrast to the developmental regulation of BEHAB/brevican, where only the secreted isoform is regulated, both isoforms are increased in parallel in human glioma. (nih.gov)
  • The distinct patterns of regulation of expression of the two isoforms suggest distinct mechanisms of regulation of BEHAB/brevican during development and in glioma. (nih.gov)
  • Here, using antisera to peptides in the predicted N-terminal and C-terminal proteolytic fragments, we demonstrate that the BEHAB/brevican protein is cleaved in invasive human and rodent gliomas. (jneurosci.org)
  • A role for this protein in glioma cell invasion was tested by transfecting a noninvasive cell line with the BEHAB/brevican gene. (jneurosci.org)
  • The noninvasive 9L glioma cell was transfected with either full-length BEHAB/brevican or the HABD and tested for invasion in in vitro and in vivo invasion assays. (jneurosci.org)
  • In every one of over 40 surgical samples of human glioma assayed to date, including oligodendroglioma, all grades of astrocytoma, and gliosarcoma, BEHAB/brevican mRNA is detected. (jneurosci.org)
  • By contrast, BEHAB/brevican is undetectable in tumors that are not of glial origin, including CNS lymphoma, meningioma, and carcinomas of the lung, colon, and breast, in either primary locations or as metastases to the brain. (jneurosci.org)
  • Binding assays with exogenously added brevican revealed that primary astrocytes and several immortalized neural cell lines have cell surface binding sites for brevican core protein. (jneurosci.org)
  • These cell surface brevican binding sites recognize the C-terminal portion of the core protein and are independent of cell surface hyaluronan. (jneurosci.org)
  • These results indicate that brevican is synthesized by astrocytes and retained on their surface by an interaction involving its core protein. (jneurosci.org)
  • These studies demonstrated that brevican is produced by astrocytes that form the neuroglial sheaths around cerebellar glomeruli and is bound to astrocytic surfaces by the interaction with "receptors" that recognizes its core protein. (jneurosci.org)
  • In their new study, the multidisciplinary team of researchers led by the Centre for Developmental Neurobiology (CDN) and MRC Centre for Neurodevelopmental Disorders (MRC CNDD) at the Institute of Psychiatry, Psychology & Neuroscience, found that this adaptability is shaped by a specific protein called Brevican. (eurekalert.org)
  • Brevican is secreted from astrocytes and neurons as a 145 kD core protein that bears up to three, covalently-linked, CS chains. (thermofisher.com)
  • Deficiency for a link protein Bral2, associated with a disruption of brevican-based axonal coats (arrow), significantly affect tissue diffusivity in the thalamic ventroposteromedial nucleus in the aged but not in the young mice. (cas.cz)
  • To gain insights into the role of brevican in brain development, we investigated its spatiotemporal expression, cell surface binding, and effects on neurite outgrowth, using rat cerebellar cortex as a model system. (jneurosci.org)
  • In this paper, we explored the physiological role of brevican by investigating its spatiotemporal expression, cell surface expression, and effects on neurite outgrowth. (jneurosci.org)
  • The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma. (genetex.com)
  • These results demonstrate for the first time that ADAMTS-5 is capable of degrading brevican and is overexpressed in glioblastoma cells, and suggest that ADAMTS-5 may play a role in glioma cell invasion through the cleavage of brevican. (elsevier.com)
  • M. G. Hamel, J. Mayer, and P. E. Gottschall, "Altered production and proteolytic processing of brevican by transforming growth factor β in cultured astrocytes," Journal of Neurochemistry , vol. 93, no. 6, pp. 1533-1541, 2005. (hindawi.com)
  • Immunoelectron microscopy revealed that brevican is localized in close association with the surface of astrocytes that form neuroglial sheaths of cerebellar glomeruli where incoming mossy fibers interact with dendrites and axons from resident neurons. (jneurosci.org)
  • In situ hybridization showed that brevican is synthesized by these astrocytes themselves. (jneurosci.org)
  • In primary cultures of cerebellar astrocytes, brevican is detected on the surface of these cells. (jneurosci.org)
  • In 293T transfectants expressing ADAMTS-4 or ADAMTS-5, brevican was cleaved into two major fragments in an identical pattern, but no such degradation was observed with ADAMTS-1 transfectants. (elsevier.com)
  • This expression pattern is further confirmed by reverse transcriptase-polymerase chain reaction (RT-PCR) experiments with RNA from different glial cell cultures, and by biochemical data demonstrating that the crude membrane fraction from isolated optic nerve contains high amounts of phosphatidylinositol-specific phospholipase C (PI-PLC)-sensitive brevican immunoreactivity. (nih.gov)
  • Immunoreactivity of brevican occurs predominantly in the protoplasmic islet in the internal granular layer after the third postnatal week. (jneurosci.org)
  • Whereas the 3.6-kb transcript encoding secreted brevican displays a widespread distribution in grey matter structures, including cerebellar and cerebral cortex, hippocampus and thalamic nuclei with silver grains accumulating over neuronal cell bodies, the smaller transcript (3.3 kb) encoding GPI-anchored isoforms appears to be largely confined to white matter tracts and diffusely distributed glial cells. (nih.gov)
  • RT "Sequence and chromosomal localization of the mouse brevican gene. (genome.jp)
  • This late appearance of GPI-linked brevican, its predominant expression in glial cells and its tight association with brain myelin fractions suggest a functional role in neuroglia. (nih.gov)
  • In this image, Brevican is expressed by two Parvalbumin cells (in red and red+blue). (eurekalert.org)
  • Purified brevican inhibits neurite outgrowth from cerebellar granule neurons in vitro , an activity that requires chondroitin sulfate chains. (jneurosci.org)
  • In contrast to the other family members, brevican occurs both as soluble isoforms secreted into the extracellular space and membrane-bound isoforms which are anchored to the cell surface via a glycosylphosphatidylinositol (GPI) moiety. (nih.gov)
  • reported that in vitro a specialized brevican-containing matrix surrounds the AIS. (rupress.org)
  • However, the expression of glypiated brevican is delayed by about 1 week, compared with the expression of the secreted isoform. (nih.gov)
  • In this new study, the researchers found that one of these proteins called Brevican, which is also one of the most abundant proteins found in the brain, influences neuronal plasticity, orchestrating a dedicated molecular program in response to changes from the environment. (eurekalert.org)
  • Temporally, the expression of brevican coincides with the maturation of glomeruli. (jneurosci.org)