Analytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesHealth Care
Branched DNA Signal Amplification AssayNucleic Acid Amplification TechniquesSelf-Sustained Sequence ReplicationSensitivity and Specificity
Branched DNA Signal Amplification Assay
A molecular probe technique that utilizes branched DNA (bDNA) as a means to amplify the hybridization signal. One end of the bDNA molecule is designed to bind a specific target, while the other end of the bDNA molecule contains many branches of DNA that are designed to bind a probe used for signal detection.
Nucleic Acid Amplification Techniques
Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.
Self-Sustained Sequence Replication
An isothermal in-vitro nucleotide amplification process. The process involves the concomitant action of a RNA-DIRECTED DNA POLYMERASE, a ribonuclease (RIBONUCLEASES), and DNA-DIRECTED RNA POLYMERASES to synthesize large quantities of sequence-specific RNA and DNA molecules.
Sensitivity and Specificity
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)

Multicenter comparison of Roche COBAS AMPLICOR MONITOR version 1.5, Organon Teknika NucliSens QT with Extractor, and Bayer Quantiplex version 3.0 for quantification of human immunodeficiency virus type 1 RNA in plasma. (1/39)

The performance and characteristics of Roche COBAS AMPLICOR HIV-1 MONITOR version 1.5 (CA MONITOR 1.5) UltraSensitive (usCA MONITOR 1. 5) and Standard (stCA MONITOR 1.5) procedures, Organon Teknika NucliSens HIV-1 RNA QT with Extractor (NucliSens), and Bayer Quantiplex HIV RNA version 3.0 (bDNA 3.0) were compared in a multicenter trial. Samples used in this study included 460 plasma specimens from human immunodeficiency virus (HIV) type 1 (HIV-1)-infected persons, 100 plasma specimens from HIV antibody (anti-HIV)-negative persons, and culture supernatants of HIV-1 subtype A to E isolates diluted in anti-HIV-negative plasma. Overall, bDNA 3.0 showed the least variation in RNA measures upon repeat testing. For the Roche assays, usCA MONITOR 1.5 displayed less variation in RNA measures than stCA MONITOR 1.5. NucliSens, at an input volume of 2 ml, showed the best sensitivity. Deming regression analysis indicated that the results of all three assays were significantly correlated (P < 0.0001). However, the mean difference in values between CA MONITOR 1.5 and bDNA 3.0 (0.274 log(10) RNA copies/ml; 95% confidence interval, 0.192 to 0.356) was significantly different from 0, indicating that CA MONITOR 1.5 values were regularly higher than bDNA 3.0 values. Upon testing of 100 anti-HIV-negative plasma specimens, usCA MONITOR 1.5 and NucliSens displayed 100% specificity, while bDNA 3.0 showed 98% specificity. NucliSens quantified 2 of 10 non-subtype B viral isolates at 1 log(10) lower than both CA MONITOR 1.5 and bDNA 3.0. For NucliSens, testing of specimens with greater than 1,000 RNA copies/ml at input volumes of 0.1, 0.2, and 2.0 ml did not affect the quality of results. Additional factors differing between assays included specimen throughput and volume requirements, limit of detection, ease of execution, instrument work space, and costs of disposal. These characteristics, along with assay performance, should be considered when one is selecting a viral load assay.  (+info)

Comparative performance of three viral load assays on human immunodeficiency virus type 1 (HIV-1) isolates representing group M (subtypes A to G) and group O: LCx HIV RNA quantitative, AMPLICOR HIV-1 MONITOR version 1.5, and Quantiplex HIV-1 RNA version 3.0. (2/39)

The performance of the LCx HIV RNA Quantitative (LCx HIV), AMPLICOR HIV-1 MONITOR version 1.5 (MONITOR v1.5), and Quantiplex HIV-1 RNA version 3.0 (bDNA v3.0) viral load assays was evaluated with 39 viral isolates (3 A, 7 B, 6 C, 4 D, 8 E, 4 F, 1 G, 4 mosaic, and 2 group O). Quantitation across the assay dynamic ranges was assessed using serial fivefold dilutions of the viruses. In addition, sequences of gag-encoded p24 (gag p24), pol-encoded integrase, and env-encoded gp41 were analyzed to assign group and subtype and to assess nucleotide mismatches at primer and probe binding sites. For group M isolates, quantification was highly correlated among all three assays. In contrast, only the LCx HIV assay reliably quantified group O isolates. The bDNA v3.0 assay detected but consistently underquantified group O viruses, whereas the MONITOR v1.5 test failed to detect group O viruses. Analysis of target regions revealed fewer primer or probe mismatches in the LCx HIV assay than in the MONITOR v1.5 test. Consistent with the high level of nucleotide conservation is the ability of the LCx HIV assay to quantify efficiently human immunodeficiency virus type 1 group M and the genetically diverse group O.  (+info)

Intra- and interlaboratory variabilities of results obtained with the Quantiplex human immunodeficiency virus type 1 RNA bDNA assay, version 3.0. (3/39)

Normal assay variation associated with bDNA tests for human immunodeficiency virus type 1 (HIV-1) RNA performed at two laboratories with different levels of test experience was investigated. Two 5-ml aliquots of blood in EDTA tubes were collected from each patient for whom the HIV-1 bDNA test was ordered. Blood was stored for no more than 4 h at room temperature prior to plasma separation. Plasma was stored at -70 degrees C until transported to the Central Pennsylvania Alliance Laboratory (CPAL; York, Pa.) and to the Hershey Medical Center (Hershey, Pa.) on dry ice. Samples were stored at < or =-70 degrees C at both laboratories prior to testing. Pools of negative (donor), low-HIV-1-RNA-positive, and high-HIV-1-RNA-positive plasma samples were also repeatedly tested at CPAL to determine both intra- and interrun variation. From 11 August 1999 until 14 September 2000, 448 patient specimens were analyzed in parallel at CPAL and Hershey. From 206 samples with results of > or =1,000 copies/ml at CPAL, 148 (72%) of the results varied by < or =0.20 log(10) when tested at Hershey and none varied by >0.50 log(10). However, of 242 specimens with results of <1,000 copies/ml at CPAL, 11 (5%) of the results varied by >0.50 log(10) when tested at Hershey. Of 38 aliquots of HIV-1 RNA pool negative samples included in 13 CPAL bDNA runs, 37 (97%) gave results of <50 copies/ml and 1 (3%) gave a result of 114 copies/ml. Low-positive HIV-1 RNA pool intrarun variation ranged from 0.06 to 0.26 log(10) while the maximum interrun variation was 0.52 log(10). High-positive HIV-1 RNA pool intrarun variation ranged from 0.04 to 0.32 log(10), while the maximum interrun variation was 0.55 log(10). In our patient population, a change in bDNA HIV-1 RNA results of < or =0.50 log(10) over time most likely represents normal laboratory test variation. However, a change of >0.50 log(10), especially if the results are >1,000 copies/ml, is likely to be significant.  (+info)

Quantitation of human immunodeficiency virus type 1 (HIV-1) RNA in cell-free cervicovaginal secretions: comparison of reverse transcription-PCR amplification (AMPLICOR HIV-A MONITOR 1.5) with enhanced-sensitivity branched-DNA assay (Quantiplex 3.0). (4/39)

Two commercially available hypersensitive assays for human immunodeficiency virus type 1 (HIV-1) RNA quantitation, AMPLICOR HIV-1 Monitor Test 1.5 and Quantiplex HIV RNA 3.0, were compared to detect and quantify HIV-1 RNA in the cell-free fraction of cervicovaginal secretions collected by vaginal washing. Three panel specimens were used: pooled cervicovaginal secretions spiked with HIV-1 subtype A or HIV-1 subtype B and cervicovaginal lavages from HIV-positive and HIV-negative women. Compared to the AMPLICOR HIV-1 Monitor Test 1.5 assay, the Quantiplex HIV-1 3.0 assay yielded higher estimates of HIV-1 RNA concentrations in several tested samples spiked with HIV-1 RNA subtype A, as well as subtype B, particularly samples containing low amounts of HIV-1 RNA. The sensitivity and specificity of the AMPLICOR HIV-1 Monitor Test 1.5 assay were 93 and 100%, respectively; the sensitivity and specificity of the Quantiplex HIV RNA 3.0 assay were 97 and 50%, respectively. In conclusion, in quantifying HIV-1 RNA in cervicovaginal secretions, the Quantiplex HIV RNA 3.0 may lack specificity, and the AMPLICOR HIV-1 Monitor Test 1.5 assay, although highly specific, may lack sensitivity.  (+info)

Comparative evaluation of the VERSANT HCV RNA 3.0, QUANTIPLEX HCV RNA 2.0, and COBAS AMPLICOR HCV MONITOR version 2.0 Assays for quantification of hepatitis C virus RNA in serum. (5/39)

A comparison of quantitative results expressed in hepatitis C virus (HCV) international units per milliliter, obtained from the VERSANT HCV RNA 3.0 (bDNA-3.0) assay, the QUANTIPLEX HCV RNA 2.0 (bDNA-2.0) assay, and the COBAS AMPLICOR HCV MONITOR version 2.0 (HCM-2.0) test was performed. A total of 168 patient specimens submitted to the Mayo Clinic Molecular Microbiology Laboratory for HCV quantification or HCV genotyping were studied. Of the specimens tested, 97, 88, and 79% yielded quantitative results within the dynamic range of the bDNA-3.0, bDNA-2.0, and HCM-2.0 assays, respectively. Overall, there was substantial agreement between the results generated by all three assays. A total of 15 out of 29 (52%) of the specimens determined to contain viral loads of <31,746 IU/ml by the bDNA-3.0 assay were categorized as containing viral loads within the range of 31,746 to 500,000 IU/ml by the bDNA-2.0 assay. Although substantial agreement was noted between the results generated by the bDNA-2.0 and bDNA-3.0 assays, a bias toward higher viral titer by the bDNA-2.0 assay was noted (P = 0.001). Likewise, although substantial agreement was noted between the results generated by the HCM-2.0 and bDNA-3.0 assays, a bias toward higher viral titer by the bDNA-3.0 assay was noted (P < or = 0.001). The discrepancy between the HCM-2.0 and bDNA-3.0 results was more pronounced when viral loads were >500,000 IU/ml and resulted in statistically significant differences (P < or = 0.001) in determining whether viral loads were above or below 800,000 IU/ml of HCV RNA, the proposed threshold value for tailoring the duration of combination therapy. The expression of quantitative values in HCV international units per milliliter was a strength of both the bDNA-3.0 and HCM-2.0 assays.  (+info)

Postnatal expression and induction by pregnenolone-16alpha-carbonitrile of the organic anion-transporting polypeptide 2 in rat liver. (6/39)

Newborn rats are more sensitive to the toxic effects of cardiac glycosides than are adult rats. This is associated with a decreased ability to remove cardiac glycosides from blood into the liver. Pregnenolone-16alpha-carbonitrile (PCN), a prototypical rodent CYP3A inducer and pregnane-X-receptor (PXR) ligand, stimulates the hepatic clearance of cardiac glycosides in newborn rats, which results in decreased toxicity of the cardiac glycosides. The mechanism responsible for this phenomenon is not clear; however, if elucidated, it would help in understanding and preventing potential drug-drug interactions. The recently cloned rat organic anion-transporting polypeptide 2 (oatp2) (Slc21a5) is a sinusoidal hepatic uptake transporter, with very high affinities for cardiac glycosides, and thus it was hypothesized that rat oatp2 increases during postnatal development and is inducible by PCN. In the present study, livers were removed from Sprague-Dawley rats from postnatal days (pnd) 0 to 45, in 5-day increments; as well as from pnd 10 to 90, in 10-day increments, after PCN (75 mg/kg i.p., for 4 days) or corn oil (vehicle for PCN) treatment. The protein and mRNA levels of rat oatp2 were determined by Western blot analysis and branched DNA signal amplification technique, respectively. Expression of rat oatp2 protein and mRNA increased gradually during postnatal development. PCN treatment increased liver to body weight ratio in both genders, and dramatically accelerated the maturation of hepatic oatp2 protein and mRNA levels. In summary, rat oatp2 undergoes age-dependent and chemical regulation during postnatal development, and is a potential target for drug-drug and age-drug interactions.  (+info)

Evaluation of the VERSANT HCV RNA 3.0 assay for quantification of hepatitis C virus RNA in serum. (7/39)

We assessed the performance of a new assay (VERSANT HCV RNA 3.0 [bDNA 3.0] assay [Bayer Diagnostics]) to quantitate HCV RNA levels and compared the results of the bDNA 3.0 assay to results of the Quantiplex HCV RNA 2.0 (bDNA 2.0) assay. Samples used in this study included 211 serum specimens from hepatitis C virus (HCV)-infected persons from two sites (Bordeaux and Marseille, France) with different genotypes; 383 serum specimens from HCV antibody-negative, HCV RNA-negative persons; and serial dilutions of World Health Organization (WHO) HCV RNA standard at a titer of 100,000 IU/ml. The specificity of the bDNA 3.0 assay was 98.2%. A high correlation was observed between expected and observed values in all dilutions of WHO standard (r = 0.9982), in serial dilutions of pooled samples (r = 0.9996), and in diluted sera from different HCV genotypes (r = 0.9930 to 0.9995). The standard deviations (SD) for the within-run and between-run reproducibility of the bDNA 3.0 assay were +info)

External quality assessment program for qualitative and quantitative detection of hepatitis C virus RNA in diagnostic virology. (8/39)

To assess the performance of laboratories in detecting and quantifying hepatitis C virus (HCV) RNA levels in HCV-infected patients, we distributed two proficiency panels for qualitative and quantitative HCV RNA testing. The panels were designed by the European Union Quality Control Concerted Action, prepared by Boston Biomedica Inc., and distributed in May 1999 (panel 1) and February 2000 (panel 2). Each panel consisted of two negative samples and six positive samples, with HCV RNA target levels from 200 to 500,000 copies/ml. Panel 1 had four samples with at least 50,000 copies/ml, and panel 2 had two samples with at least 50,000 copies/ml. Fifty-seven laboratories submitted 45 qualitative and 35 quantitative data sets on panel 1, and 81 laboratories submitted 75 qualitative and 48 quantitative data sets on panel 2. In both panels, about two-thirds of the qualitative data sets and >90% of the quantitative data sets were obtained with commercial assays. With each panel, two data sets gave one false-positive result, corresponding to false-positivity rates of 1.3% and 0.8% for panel 1 and panel 2, respectively. Samples containing at least 50,000 copies/ml were found positive in 97% and 99% of the cases with panel 1 and panel 2, respectively. In contrast, the positive samples containing < or =5,000 copies/ml were reported positive in only 71% and 77% of the cases with panel 1 and panel 2, respectively. Adequate or better scores on qualitative results (all results correct or only the low-positive samples missed) were obtained in 84% (panel 1) and 80% (panel 2) of the data sets. In the analysis of quantitative results, 60% (panel 1) and 73% (panel 2) of the data sets obtained an adequate or better score (> or =80% of the positive results within the range of the geometric mean +/- 0.5 log(10)). Our results indicate that considerable improvements in molecular detection and quantitation of HCV have been achieved, particularly through the use of commercial assays. However, the lowest detection levels of many assays are still too high, and further standardization is still needed. Finally, this study underlines the importance of proficiency panels for monitoring the quality of diagnostic laboratories.  (+info)

VERSANT HCV RNA 1.0 Assay (kPCR) - Siemens Healthineers ÖsterreichVERSANT HCV RNA 1.0 Assay (kPCR) - Siemens Healthineers Österreich
The VERSANT HCV RNA 1.0 Assay is a real-time kPCR assay for quantitative detection of human HCV RNA in plasma or serum of HCV-infected individuals.
https://www.healthcare.siemens.at/molecular-diagnostics/molecular-diagnostics-in-vitro-diagnostics/versant-hcv-rna-10-assay
VERSANT HCV RNA 1.0 Assay (kPCR) - Siemens Healthineers ÖsterreichVERSANT HCV RNA 1.0 Assay (kPCR) - Siemens Healthineers Österreich
The VERSANT® HCV RNA 1.0 Assay (kPCR)* is a real-time kinetic polymerase chain reaction (kPCR) assay for quantitative detection of human hepatitis C virus (HCV) RNA in plasma or serum of HCV-infected individuals. The assay is intended to be used in conjunction with clinical presentation and other laboratory markers of disease status to aid in the management of HCV-infected individuals undergoing antiviral therapy.. ...
https://www.healthcare.siemens.at/molecular-diagnostics/molecular-diagnostics-in-vitro-diagnostics/versant-hcv-rna-10-assay/technical-details
VERSANT HCV RNA 1.0 Assay (kPCR) - Siemens Healthineers SuisseVERSANT HCV RNA 1.0 Assay (kPCR) - Siemens Healthineers Suisse
HCV infection is one of the main causes of chronic liver disease worldwide.1 Fortunately, with detection and appropriate therapy, the majority of HCV-infected individuals can be cured. The European Association for the Study of the Liver (EASL) recommends response-guided therapy for HCV, which includes the monitoring of treatment efficacy using a real-time PCR-based assay, with a lower limit of detection of 10-20 IU/mL.2 The VERSANT® HCV RNA 1.0 Assay (kPCR)*, which has a limit of detection of 15 IU/mL, provides excellent precision across the dynamic range and 100 percent specificity. Additionally, the assay features technology that is compatible with all HCV genotypes, providing clinicians the information that they need to manage patient therapy.. 1. Lavanchy D. The global burden of hepatitis C. Liver Int 2009; 29:74-81 ...
https://www.healthcare.siemens.ch/fr/molecular-diagnostics/molecular-diagnostics-in-vitro-diagnostics/versant-hcv-rna-10-assay/features
Performance of point-of-care Xpert HIV-1 plasma viral load assay at a tertiary HIV care centre in Southern India. | Physicians...Performance of point-of-care Xpert HIV-1 plasma viral load assay at a tertiary HIV care centre in Southern India. | Physicians...
The performance of the point-of-care Xpert HIV-1 viral load assay was evaluated against the Abbott RealTime PCR m2000rt system. A total of 96 plasma specimens ranging from 2.5 log10 copies ml-1 to 4.99 log10 copies ml-1 and proficiency testing panel specimens were used. Precision and accuracy were checked using the Pearson correlation co-efficient test and Bland-Altman analysis.. RESULTS ...
https://www.physiciansweekly.com/performance-of-point-of-care-xpert-hiv-1-plasma-viral-load-assay-at-a-tertiary-hiv-care-centre-in-southern-india/
Difference between revisions of User:Jakob G. Wells - OpenWetWareDifference between revisions of User:Jakob G. Wells - OpenWetWare
PCR detection works by heating the DNA sample to about 110°C in order to split the DNA. Then the PCR cools off to 57°C in order for the primer to attach to the DNA strands. The PCR then heats to 72°C so the DNA strand can be re-written. The r17879961 cancer-associated sequence will produce a DNA signal because the reverse primer used, AACTCTTACACTCGATACAT will only attach if the DNA sample has the same coding with the cancer-associated sequence ACT. If the DNA sample does not have the cancer-associated sequence the primer will not attach and there will be no DNA signal.,br ...
https://openwetware.org/wiki/?title=User:Jakob_G._Wells&diff=656725&oldid=650250
Investigator Quantiplex Pro Kit - QIAGEN Online ShopInvestigator Quantiplex Pro Kit - QIAGEN Online Shop
Investigator Quantiplex Pro Kit, For quantification of human and male DNA with high sensitivity and a straightforward quality assessment
https://www.qiagen.com/au/shop/new-products/investigator-quantiplex-pro-kit/
VERSANT HCV Genotype 2.0 assay (LiPA) - Siemens Healthineers Česká republika
IVDD, CE marked. Product availability varies from country to country and is subject to local regulatory requirements.. VERSANT is a registered trademark of Siemens. All other marks are the property of their respective owners.. The products/features (mentioned herein) are not commercially available in all countries. Due to regulatory reasons their future availability cannot be guaranteed. Please contact your local Siemens organization for further details. ...
https://www.healthcare.siemens.cz/molecular-diagnostics/molecular-diagnostics-in-vitro-diagnostics/versant-hcv-genotype-2-assay-lipa/features
PRTG Network Monitor - Version HistoryPRTG Network Monitor - Version History
Since 1997, we offer monitoring solutions for businesses across all industries and all sizes, from SMB to large enterprises. We believe monitoring plays a vital part in reducing humankinds consumption of resources. Our products help our customers optimize their IT, OT and IoT infrastructures, and reduce their energy consumption or emissions - for our future and our environment.. ...
https://www.paessler.com/it/prtg/history/stable
VERSANT HCV Genotype Test - Siemens Healthineers Česká republika
The products/features (mentioned herein) are not commercially available in all countries. Due to regulatory reasons their future availability cannot be guaranteed. Please contact your local Siemens organization for further details. ...
https://www.healthcare.siemens.cz/molecular-diagnostics/molecular-diagnostics-systems/versant-hcv-genotype-2-products/assays
Lab using a new assay - Forum on Understanding Your Labs -- TheBody.comLab using a new assay - Forum on Understanding Your Labs -- TheBody.com
Likely a new method, although hard to be certain. This definitely happens when labs go to a new viral load assay. Depending on the technology used, some recalibration will be required, either up or...
http://www.thebody.com/Forums/AIDS/Labs/Q191359.html
The Review E-newsletter design by super things | ThemeForestThe Review E-newsletter design by super things | ThemeForest
Buy The Review E-newsletter design by super_things on ThemeForest. About The Review An elegant and clean layout, ideal for business and commercial use. Special Campaign Monitor version...
https://themeforest.net/item/the-review-enewsletter-design/551476?WT.ac=category_item&WT.seg_1=category_item&WT.z_author=super_things
One-Step TaqTicProbe qRT-PCR iCycler - Biocrede Inc.One-Step TaqTicProbe qRT-PCR iCycler - Biocrede Inc.
Product Description Convenient real-time RNA quantification in one EASY step. Biocredes One-Step TaqTicProbe qRT-PCR iCycler Kit uses a combination of high-qua
https://www.biocrede.com/products/one-step-taqticprobe-qrt-pcr-icycler
Branched DNA assay - WikipediaBranched DNA assay - Wikipedia
In biology, a branched DNA assay is a signal amplification assay (as opposed to a target amplification assay) that is used to detect nucleic acid molecules. A branched DNA assay begins with a dish or some other solid support (e.g., a plastic dipstick). The dish is peppered with small, single stranded DNA molecules (or chains) that stick up into the air. These are known as capture probe DNA molecules. Next, an extender DNA molecule is added. Each extender has two domains; one that hybridizes to the capture DNA molecule and one that hangs out in the air. The purpose of the extender is two-fold. First, it creates more available surface area for target DNA molecules to bind, and second, it allows the assay to be easily adapted to detect a variety of target DNA molecules. Once the capture and extender molecules are in place and they have hybridized, the sample can be added. Target molecules in the sample will bind to the extender molecule. This results in a base peppered with capture probes, ...
https://en.wikipedia.org/wiki/Branched_DNA_assay
Serval - Performance of five different assays for the quantification of viral load in persons infected with various subtypes of...Serval - Performance of five different assays for the quantification of viral load in persons infected with various subtypes of...
Five methods for the assessment of plasma viral load (VL) were evaluated in 103 seropositive patients infected with various subtypes of HIV-1. The methods included three RNA-based assays (Amplicor Monitor 1.5, Quantiplex version 2.0, NucliSens), one ultrasensitive reverse transcriptase (PERT) assay and one boosted p24 antigen (Ag) enzyme immunoassay (EIA). Subtyping was based on sequencing in env. The sensitivities were, in decreasing order, Amplicor | PERT | p24 Ag | NucliSens | Quantiplex. The low sensitivity of NucliSens was related to the missing of several non-B (A, E, F, G) or recombinant strains, whereas that of Quantiplex did not depend on subtype. In the 1 group O sample and 4 group M samples, only PERT assay or p24Ag EIA produced a positive result. In the quantitative range, correlation was best between Amplicor and Quantiplex (r = 0.8848), fair between Amplicor and NucliSens (r = 0.7064) or PERT assay (r = 0.7266), lowest between Amplicor and p24Ag EIA (r = 0.3989). Amplic
https://serval.unil.ch/fr/notice/serval:BIB_38A276F2AF1E
Branched-DNA Assay for Detection of themecA Gene in Oxacillin-Resistant and Oxacillin-Sensitive Staphylococci | Journal of...Branched-DNA Assay for Detection of themecA Gene in Oxacillin-Resistant and Oxacillin-Sensitive Staphylococci | Journal of...
In a previous study, we evaluated a mecA-specific assay that used a paramagnetic particle-labeled probe to separate the target-probe duplexes from solution and an acridinium ester-labeled probe to detect the hybridized mecA gene (11). However, the assay was limited by low sensitivity and high concentrations of organism were needed to obtain a valid result. The MecA bDNA assay affords a significant increase in sensitivity that is probably due to the inherent signal amplification properties of the assay. When the PCR was used as the gold standard, the bDNA assay was 100% sensitive and specific for both S. aureus and CNS.. Among 416 isolates, only 11 gave discrepant results between genotypic and phenotypic assays. Of these discrepant isolates, 10 contained themecA gene yet were phenotypically sensitive to oxacillin and 1 did not contain the mecA gene but was oxacillin resistant. Eight of these MecA+ isolates were later identified asS. epidermidis, and two were identified as S. hominis. The MecA− ...
https://jcm.asm.org/content/36/9/2640
VERSANT HIV-1 RNA 1.0 Assay (kPCR) - Siemens Healthineers SuisseVERSANT HIV-1 RNA 1.0 Assay (kPCR) - Siemens Healthineers Suisse
The VERSANT HIV-1 RNA 1.0 Assay (kPCR) provides efficient and reproducible extraction, amplification, and detection of HIV-1 RNA.
https://www.healthcare.siemens.ch/fr/molecular-diagnostics/molecular-diagnostics-in-vitro-diagnostics/versant-hiv-1-rna-1-assay/features
Versant 3100 Press Meets Printing Needs - XeroxVersant 3100 Press Meets Printing Needs - Xerox
This white paper can help you determine if the Versant 3100 Press can meet the printing needs of your business with its very advanced automation
https://www.xerox.com/en-us/digital-printing/insights/versant-3100-meets-printing-needs
Patent US6020132 - Method of analysis using signal amplification - Google PatentsPatent US6020132 - Method of analysis using signal amplification - Google Patents
A method for determining a molecule A comprising a sample of molecules capable of participating in formation of triplex structures is useful for sensitive determination. The principle can be used to determine any kind of analytes.
http://www.google.com/patents/US6020132?dq=6,370,566
Different Hepatitis C Virus (HCV) RNA Load Profiles Following Seroconversion among Injecting Drug Users without Correlation...Different Hepatitis C Virus (HCV) RNA Load Profiles Following Seroconversion among Injecting Drug Users without Correlation...
Since HCV infection often persists in association with chronic hepatitis, it is appropriate to consider the natural history of HCV. We must determine what factors determine the clinical outcome of HCV infection and whether normalization of liver enzymes, with or without the presence of HCV RNA, is of any importance. The diagnosis and monitoring of HCV infection have become easier and more accurate with the quantitative and qualitative amplification techniques now available. However, the clinical relevance of the presence of HCV RNA, in correlation with serum ALT levels and HCV genotypes, is still not fully understood (18, 19).. Our study shows a very high concordance, 98.9%, between the bDNA assay and our single-step RT-PCR. Both assays target the same highly conserved region of HCV. The bDNA assay is highly reproducible and is unaffected by the genotypic variability of HCV (8); it is therefore a useful tool for monitoring HCV RNA levels throughout the course of disease. Our longitudinal ...
https://jcm.asm.org/content/36/4/872
Point-of-care HIV viral load assays to simplify care | CAPRISA
In this study, Garrett et al report on their early clinical experience evaluating the point-of-care Xpert® HIV-1 VL assay, which is processed on the GeneXpert® System (Cepheid, Sunnydale, California, USA) against the gold-standard Roche Taqman version 2 assay (Roche Diagnostics, Switzerland). The Xpert® HIV-1 VL is a fully automated real-time molecular cartridge-based assay with a linear range of 40 to 10 million copies/ml of HIV RNA, and can be run in a clinical setting providing a result within 90 minutes.. Investigating samples from 42 women participating in the CAPRISA 002 Acute Infection Study, Xpert® HIV-1 VL correlated strongly with the Taqman assay across the VL spectrum (Spearman ?=0.94, p,0.001, Figure 1). A Bland-Altman plot showed a mean difference between Taqman and Xpert® results of -0.10 log copies/ml (95% limits of agreement -0.59 to 0.39) with slightly higher values on Xpert®. Importantly, only one woman was misclassified using a VL threshold of 1000 copies/ml, the current ...
https://www.caprisa.org/NewsAndEvents/Read/10288
Výsledky VaV - VUT v BrněVýsledky VaV - VUT v Brně
article{BUT115093, author=Karel {Sedlář} and Helena {Škutková} and Martin {Vítek} and Ivo {Provazník}, title=Set of rules for genomic signal downsampling, annote=Comparison and classification of organisms based on molecular data is an important task of computational biology, since at least parts of DNA sequences for many organisms are available. Unfortunately, methods for comparison are computationally very demanding, suitable only for short sequences. In this paper, we focus on the redundancy of genetic information stored in DNA sequences. We proposed rules for downsampling of DNA signals of cumulated phase. According to the length of an original sequence, we are able to significantly reduce the amount of data with only slight loss of original information. Dyadic wavelet transform was chosen for fast downsampling with minimum influence on signal shape carrying the biological information. We proved the usability of such new short signals by measuring percentage deviation of pairs of ...
https://www.vutbr.cz/vav/vysledky/detail?vav_id=115093
OC-034 Identifying Patients with Elevated Circulating Bacterial DNA May Reduce Infection and Death in Alcoholic Hepatitis | GutOC-034 Identifying Patients with Elevated Circulating Bacterial DNA May Reduce Infection and Death in Alcoholic Hepatitis | Gut
Results bDNA was quantified in 714 whole blood samples. There was no correlation between bDNA levels and baseline characteristics such as age and gender, nor with static markers of liver function such as MELD. Elevated bDNA levels were associated with development of infection within the first 7 days in patients treated with prednisolone (p = 0.001), and remained independently associated with the subsequent development of infection after multivariate analysis (p = 0.02). Importantly, bDNA levels were not associated with the development of infection in patients treated without prednisolone (p = 0.43).. Elevated bDNA levels were associated with death at 90 days (p = 0.05). Mortality in prednisolone treated patients was increased for patients with high bDNA compared to patients with low bDNA (hazard ratio 2; p = 0.02). Accordingly, patients with elevated bDNA were more likely to die if they were randomly allocated prednisolone therapy (OR 2.9, 95% CI 1.2-6.9, p = 0.02). We estimate that if patients ...
http://gut.bmj.com/content/65/Suppl_1/A20.2
Comparison of two methods for quantification of hepatitis B viral DNAComparison of two methods for quantification of hepatitis B viral DNA
These findings suggest that the recently developed cross-linking assay is more sensitive than the bDNA assay for the quantitative determination of HBV-DNA.
https://pubmed.ncbi.nlm.nih.gov/10355505/?dopt=Abstract
what do these blips mean? - Forum on Understanding Your Labs -- TheBody.comwhat do these blips mean? - Forum on Understanding Your Labs -- TheBody.com
You have made the correct diagnosis. Blipping is usually caused by issues associated with the viral load assay or more specifically with the blood collection tube that the sample is collected in. In...
http://www.thebody.com/Forums/AIDS/Labs/Q168601.html
outside front cover] A label-free and enzyme-free signal amplification strategy for a sensitive RNase H activity assay -...
Title: Alabel-free and enzyme-free signal amplification strategy for a sensitive RNase Hactivity assay Author: Chang Yeol Lee,‡Hyowon Jang,‡ Ki Soo Park* and Hyun Gyu Park* (‡: equal contribution) Journal: Nanoscale 2017, 9, 16149-16153 Abstra
http://cbe.kaist.ac.kr/insiter.php?design_file=983.php&article_num=75&PB_1423038331=11
outside front cover] A label-free and enzyme-free signal amplification strategy for a sensitive RNase H activity assay -...
Title: Alabel-free and enzyme-free signal amplification strategy for a sensitive RNase Hactivity assay Author: Chang Yeol Lee,‡Hyowon Jang,‡ Ki Soo Park* and Hyun Gyu Park* (‡: equal contribution) Journal: Nanoscale 2017, 9, 16149-16153 Abstra
http://cbe.kaist.ac.kr/insiter.php?design_file=983.php&article_num=75&PB_1423038331=10
VERSANT CT/GC DNA Test - Siemens Healthineers Argentina
Ready and easy-to-use VERSANT Urine Transport Kit (UTK) requires no manual pipetting, centrifugation, or off-line processing steps ...
https://www.healthcare.siemens.com.ar/molecular-diagnostics/molecular-diagnostics-in-vitro-diagnostics/versant-ct-gc-dna-1/features-benefits
Ecostead Versant - Alembic Studio
The project is comprised of a number of discrete and overlapping components- guest quarters, office space, a four seasons classroom, greenhouse, commercial kitchen, fitness room, and several gathering spaces. Heating and cooling for the home is provided by complementary systems, comprised of passive elements, active solar thermal and photovoltaic panels. Given the intrinsic permaculture component, it was decided to utilize only surface and rainwater for the landscaping; with a combination of ponds and cisterns, there is provision for over 170,000 gallons of water storage on the site.. #gallery-2 { margin: auto; } #gallery-2 .gallery-item { float: left; margin-top: 10px; text-align: center; width: 33%; } #gallery-2 img { border: 2px solid #cfcfcf; } #gallery-2 .gallery-caption { margin-left: 0; } /* see gallery_shortcode() in wp-includes/media.php */ ...
https://alembicstudio.com/projects/ecostead-versant/
Customized Assays - Microsynth - CHCustomized Assays - Microsynth - CH
Microsynth, a leading provider of molecular biology services, offers a broad range of PCR services and products. More than 20 years of experience with customer projects of any size, our in-depth understanding of DNA/RNA quantification as well as our in-house production facility for primer & probes qualify Microsynth as your partner of choice for the development and validation of any PCR-based assay. Microsynth is also a competent and reliable partner for the subsequent testing phase, regardless of scale.
https://www.microsynth.com/customized-assays.html
6 Ways BDNA Improves Your Cybersecurity Posture -- FCW6 Ways BDNA Improves Your Cybersecurity Posture -- FCW
To proactively address cybersecurity risk, federal agencies need comprehensive visibility into their IT environments and actionable data to prioritize risk-mitigation activities. These six capabilities will help do that.
https://fcw.com/whitepapers/2017/06/bdna-6-ways-improve-cybersecurity-posture-060117.aspx
Mechanisms of Cell Signaling | Medical Online Video Lecture | sqadia.comMechanisms of Cell Signaling | Medical Online Video Lecture | sqadia.com
Cell Biology Lecture on types of receptors and signaling, GPCR, Ach, signal amplification and transduction along with the cell surface receptors and their functions.
https://www.sqadia.com/programs/mechanism-of-cell-signaling
Evaluation of Versant hepatitis C virus genotype assay (LiPA) 2.0 | Read by QxMDEvaluation of Versant hepatitis C virus genotype assay (LiPA) 2.0 | Read by QxMD
Hepatitis C virus (HCV) genotyping is a tool used to optimize antiviral treatment regimens. The newly developed Versant HCV genotype assay (LiPA) 2.0 uses sequence information from both the 5 untranslated region and the core region, allowing distinction between HCV genotype 1 and subtypes c to l of genotype 6 and between subtypes a and b of genotype 1. HCV-positive samples were genotyped manually using the Versant HCV genotype assay (LiPA) 2.0 system according to the manufacturers instructions. For the comparison study, Versant HCV genotype assay (LiPA) 1.0 was used. In this study, 99.7% of the samples could be amplified, the genotype of 96.0% of samples could be determined, and the agreement with the reference method was 99.4% when a genotype was determined. The reproducibility study showed no significant differences in performance across sites (P = 0.43) or across lots (P = 0.88). In the comparison study, 13 samples that were uninterpretable or incorrectly genotyped with Versant HCV genotype ...
https://read.qxmd.com/read/18400913/evaluation-of-versant-hepatitis-c-virus-genotype-assay-lipa-2-0
Performance Comparison of Xpert HIV-1 Viral Load Assay and Roche Taqman and Abbott M2000 RT in Bamako, Mali | AbstractPerformance Comparison of Xpert HIV-1 Viral Load Assay and Roche Taqman and Abbott M2000 RT in Bamako, Mali | Abstract
Background: Routine monitoring of HIV-1 Viral Load (VL) is important in patients on Antiretroviral Therapy (ART) management. Access to HIV VL remains ..
https://www.hilarispublisher.com/abstract/performance-comparison-of-xpert-hiv1-viral-load-assay-and-roche-taqman-and-abbott-m2000-rt-in-bamako-mali-46953.html
Most recent papers with the keyword Tumor-derived exosomes | Read by QxMDMost recent papers with the keyword Tumor-derived exosomes | Read by QxMD
Tumor-derived exosomes (TEXs) are extracellular vesicles that are continuously released into the blood by tumor cells and carry specific surface markers of the original tumor cells. Substantial evidence has implicated TEXs as attractive diagnostic markers for cancer. However, the detection of TEXs in blood at an early tumor stage is challenging due to their very low concentration. Here, we established a method called PLA-RPA-TMA assay that allows TEXs to be detected with high sensitivity and specificity. Based on two proximity ligation assay (PLA) probes that recognize a biomarker on a TEX, we generated a unique surrogate DNA signal for the specific biomarker, which was synchronously amplified twice by recombinase polymerase amplification (RPA) coupled with transcription-mediated amplification (TMA), and then the products of the RPA-TMA reaction were quantitatively detected using a gold nanoparticle-based colorimetric assay ...
https://www.readbyqxmd.com/keyword/73941
Biological dynamics of viral load in hemodialysis patients with HCV infection: Analysis by branched -chain DNA (bDNA) signal...
Fingerprint Dive into the research topics of Biological dynamics of viral load in hemodialysis patients with HCV infection: Analysis by branched -chain DNA (bDNA) signal amplification assay. Together they form a unique fingerprint. ...
https://miami.pure.elsevier.com/en/publications/biological-dynamics-of-viral-load-in-hemodialysis-patients-with-h-2/fingerprints/
VERSANT Tissue Prep KitVERSANT Tissue Prep Kit
A informação que se segue destina-se, a profissionais de saúde, em conformidade com o disposto no art.º 3.º, n.º 1, alínea ggg) do Decreto-Lei n.º 176/2006, de 30 de Agosto (Estatuto do Medicamento). Se é um profissional de saúde clique OK, caso contrário clique Cancel. A identificação como profissional de saúde, para aceder à informação que se segue, é da sua exclusiva responsabilidade. ...
https://www.siemens-healthineers.com/pt/molecular-diagnostics/molecular-diagnostics-in-vitro-diagnostics/tissue-prep-reagents
Hepatitis C virus RNA quantification in right and left lobes of the liver in patients with chronic hepatitis C<...
TY - JOUR. T1 - Hepatitis C virus RNA quantification in right and left lobes of the liver in patients with chronic hepatitis C. AU - Idrovo, V.. AU - Dailey, P. J.. AU - Jeffers, Lennox J. AU - Coelho-Little, E.. AU - Bernstein, D.. AU - Bartholomew, M.. AU - Alvarez, L.. AU - Urdea, M. S.. AU - Collins, M. L.. AU - Schiff, Eugene R. PY - 1996/9/1. Y1 - 1996/9/1. N2 - Quantification of hepatitis C virus RNA in liver tissue is likely to be useful in the study of the natural history, pathogenesis, progression and treatment of hepatitis C virus-associated liver disease. Quantitative measurements of hepatitis C virus RNA in liver biopsy samples using the branched DNA (bDNA) signal amplification assay were carried out. The aims of this study were threefold: first, to assess the level of hepatitis C virus RNA in biopsy samples from the right and left lobes of the liver; second, to evaluate the correlation between hepatitis C virus RNA levels in serum and liver; and third, to investigate the ...
https://miami.pure.elsevier.com/en/publications/hepatitis-c-virus-rna-quantification-in-right-and-left-lobes-of-t
Utility of random amplification of polymorphic DNA assay and B...Utility of random amplification of polymorphic DNA assay and B...
Utility of random amplification of polymorphic DNA assay and BOX-A PCR in molecular characterization of Streptococcus pneumoniae isolates recovered from various
https://www.mysciencework.com/publication/show/utility-random-amplification-polymorphic-dna-assay-box-pcr-molecular-characterization-streptococcus-pneumoniae-isolates-d99b2093
16 x PERSONA Monitor Test Sticks for the NEW and OLD Monitor | Home Health UK16 x PERSONA Monitor Test Sticks for the NEW and OLD Monitor | Home Health UK
16 x PERSONA MONITOR TEST STICKS for the NEW and OLD Persona Monitor These Persona monitor sticks are for use with the NEW and the OLD Persona ovulation
https://homehealth-uk.com/all-products/16-x-persona-test-sticks-new-and-old-monitor/
Grand Colon : Versant W (Voie Normale) - Camptocamp.orgGrand Colon : Versant W (Voie Normale) - Camptocamp.org
Javais prévu daller vers la Croix de Belledonne, mais lenneigemment est encore énorme en cette fin mai. Et donc pour éviter une longue galère dans de la neige molle je me tourne vers le Grand Colon, plus proche et plus facile daccès ...
https://www.camptocamp.org/outings/221618/fr/grand-colon-versant-w-voie-normale
CFAS HBA1C - 3X2ml for Roche Cobas Integra 400 / 400+ - BiopromCFAS HBA1C - 3X2ml for Roche Cobas Integra 400 / 400+ - Bioprom
We Provide Technical & Scientific Support for all analyzers we trade. Ask us for a price or to send you the (manual). See all Roche Reagents
https://bioprom.gr/en/product/calibrator-cfas-hba1c-3x2ml/
Leader LV5700A - Service Monitors Other Types of EquipmentLeader LV5700A - Service Monitors Other Types of Equipment
Leader LV5700A The LV 5700A is a Multi SDI Monitor for HD/SD-SDI signals with an XGA TFT color LCD in an adjustable tilt front panel. The monitor tests 14 HD-SDI and SD-SDI formats with total digital processing c
https://www.testequipmentconnection.com/39605/Leader_LV5700A.php
Download Differential Diagnosis in Surgical Pathology: Expert Consult by Paolo Gattuso MD, Vijaya B. Reddy MD, Odile David MD,...Download Differential Diagnosis in Surgical Pathology: Expert Consult by Paolo Gattuso MD, Vijaya B. Reddy MD, Odile David MD,...
San Diego, CA. FDA-cleared TMA tests are available for the detection of Chlamydia trachomatis, Neisseria gonorrhoeae, and Mycobacterium tuberculosis (APTIMA CT, APTIMA GC, and, AMPLIFIED Mycobacterium tuberculosis Direct Test [MTD] assays, respectively) An FDA-approved TMA qualitative assay for hepatitis C virus (HCV) is also available (VERSANT HCV RNA [distributed by Siemens Healthcare Diagnostics, Deerfield, IL]) - Nucleic acid sequence-based amplification (NASBA) ◆ NASBA is an isothermal amplification technique and can be used for the amplification of a DNA or RNA target. Following the first PCR cycle, there is (theoretically) a per-cycle doubling in the number of copies of the PCR product. (Modified from Leonard DGB [ed]: Diagnostic Molecular Pathology. ) - Basic PCR method ◆ During the denaturation stage, sample specimen DNA is rendered single-stranded by heating to 94° to 98°C ◆ In the annealing step, oligonucleotide primers hybridize with the target sequences they have been ...
http://jakebellows.com/lib/differential-diagnosis-in-surgical-pathology-expert-consult-online-and-print
Evaluation of the Xpert HCV Viral Load Finger-Stick point-of-care assay<...Evaluation of the Xpert HCV Viral Load Finger-Stick point-of-care assay<...
TY - JOUR. T1 - Evaluation of the Xpert HCV Viral Load Finger-Stick point-of-care assay. AU - Lamoury, Francois M. J.. AU - Bajis, Sahar. AU - Hajarizadeh, Behzad. AU - Marshall, Alison D.. AU - Martinello, Marianne. AU - Ivanova, Elena. AU - Catlett, Beth. AU - Mowat, Yasmin. AU - Marks, Philippa. AU - Amin, Janaki. AU - Smith, Julie. AU - Ezard, Nadine. AU - Cock, Victoria. AU - Hayllar, Jeremy. AU - Persing, David H.. AU - Kleman, Marika. AU - Cunningham, Philip. AU - Dore, Gregory J.. AU - Applegate, Tanya L.. AU - Grebely, Jason. AU - LiveRLife Study Group. PY - 2018/5/25. Y1 - 2018/5/25. N2 - Point-of-care hepatitis C virus (HCV) RNA testing is advantageous, enabling diagnosis of active infection in a single visit. This study evaluated the sensitivity and specificity of the Xpert HCV Viral Load Finger-Stick assay (Xpert HCV VL FS) for HCV RNA detection (finger-stick) and the Xpert HCV Viral Load assay (plasma) compared with the Abbott RealTime HCV Viral Load assay by venepuncture. Plasma ...
https://researchers.mq.edu.au/en/publications/evaluation-of-the-xpert-hcv-viral-load-finger-stick-point-of-care
Misdiagnosis of HIV Infection | Annals of Internal Medicine | American College of PhysiciansMisdiagnosis of HIV Infection | Annals of Internal Medicine | American College of Physicians
When a test has more than two possible outcomes, its accuracy can be reported as pairs of sensitivity and specificity corresponding to each degree of abnormality. This approach, the basis for receiver-operating characteristic analysis, maximizes the use of diagnostic information (2). When the diagnostic threshold is set at a lower degree of abnormality, the sensitivity of the test tends to increase but its specificity tends to decrease. The opposite occurs when a higher diagnostic threshold is selected. In the case of HIV viral load assays, if more viral units must be detected to report the test result as abnormal, the specificity will increase. As noted by Rich and colleagues, the lowest reported plasma viral load during seroconversion is more than 17 times higher than the highest viral load detected in our three patients. Thus, for the diagnosis of acute infection, the threshold should probably be set much higher ...
http://annals.org/aim/article-abstract/712999/misdiagnosis-hiv-infection
Citizen News Service - CNS: Point-of-care HIV testing: Important cog in the wheel towards ending AIDS by 2030Citizen News Service - CNS: Point-of-care HIV testing: Important cog in the wheel towards ending AIDS by 2030
Dr Ellen Jo Baron, Emeritus Professor of Pathology at the Stanford University Medical Center and associated with Cepheid, gave the example of Cepheids Xpert HIV1 Viral Load assay which is strictly not a point-of-care test. But it can be done in a small laboratory that has necessary resources. It is fully automated, can identify all the sub-types and gives results in 90 minutes. Dr Baron shared that Cepheid has got a grant to create a finger-stick test for viral load testing, which is battery operated, portable, rugged, relatively affordable, can be run on omni instrument at remote sites. It is hoped to give results in less than 60 minutes, with an accuracy of 500 copies/ml ...
http://www.citizen-news.org/2016/10/point-of-care-hiv-testing-important-cog.html
KR101508835B1 - Power detecting circuit and rf signal amplification circuit having thereof - Google Patents
The present invention relates to a power detection circuit and an RF signal amplification circuit including the same. According to one embodiment of the present invention, there is provided a RF matching amplifier comprising: a coupling portion adjacent to an RF matching inductor and extracting induced power; A rectifying unit for rectifying and outputting a signal output from the coupling unit; A slope adjusting unit connected between an output terminal of the rectifying unit and the ground and changing an output signal of the output terminal of the rectifying unit according to a change in internal impedance to adjust a voltage slope for power detection; And a smoothing unit for smoothing the output signal of the output terminal of the rectifying unit into a DC voltage for power detection using a voltage slope. Further, an RF signal amplifying circuit having the RF signal amplifying circuit is proposed.
https://patents.google.com/patent/KR101508835B1/en
Bilirubin Linearity Verifier (Roche Cobas) - Randox Online StoreBilirubin Linearity Verifier (Roche Cobas) - Randox Online Store
Our Bilirubin verifier contains both Direct Bilirubin and Total Bilirubin so testing is fully covered. Dedicated for use on Roche Cobas systems, this verifier coming in a lyophilised format and spans five levels ensuring the instruments entire reportable range is measured. ...
https://store.randox.com/product/bilirubin-linearity-verifier/
Holter Monitor Test Los Angeles, Heart Check-Up Century City 90067
Make an appointment for the Holter Monitor Test with Dr. Roya Golshani if you have symptoms, such as irregular palpitations/ heartbeats, chest pain, and shortness of breath.
http://healthylawoman.com/holter-monitor.php
Growth Hormone in the Treatment of HIV-Associated Wasting - Full Text View - ClinicalTrials.govGrowth Hormone in the Treatment of HIV-Associated Wasting - Full Text View - ClinicalTrials.gov
Have clearly documented HIV infection, determined by the presence of HIV confirmed by one of the following: Western blot, immunofluorescence assay, HIV culture, polymerase chain reaction (PCR) amplification, branched DNA (bDNA) signal amplification or the presence of p24 antigen. These tests may have been performed at any time in the past, but the results must be available for review by Serono prior to entry into the study ...
https://clinicaltrials.gov/ct2/show/NCT00489528?term=%22+May+22%2C++2007%22%3A%22+June+22%2C++2007%22%5BFIRST-RECEIVED-DATE%5DAND+HIV%5BCONDITION%5D&rank=3
Growth Hormone in the Treatment of HIV-Associated Wasting - Full Text View - ClinicalTrials.govGrowth Hormone in the Treatment of HIV-Associated Wasting - Full Text View - ClinicalTrials.gov
Have clearly documented HIV infection, determined by the presence of HIV confirmed by one of the following: Western blot, immunofluorescence assay, HIV culture, polymerase chain reaction (PCR) amplification, branched DNA (bDNA) signal amplification or the presence of p24 antigen. These tests may have been performed at any time in the past, but the results must be available for review by Serono prior to entry into the study ...
https://clinicaltrials.gov/show/NCT00489528?order=51
Monitor Test Measurement | LORD Sensing SystemsMonitor Test Measurement | LORD Sensing Systems
MicroStrains cost-effective wireless sensing solutions enable a broad suite of monitoring, testing, and measurement applications. Our expertise in long-range wireless sensor communication, progressive power management, and cloud-based sensor data management enable accessible, long-term monitoring of machines, buildings, vehicles, bridges, wind turbines, conveyor belts and other high value assets.. With MicroStrains wireless sensor networks, structural health monitoring, condition based maintenance, and predictive maintenance can help ensure integrity, safety, and reliability of machines, buildings and structures and more while efficiently and cost-effectively assessing the long-term performance of structure and machine components and preventing costly repairs and downtime.. ...
https://www.microstrain.com/applications/monitor-test-measurement
Branched DNA assayBranched DNA assay
In biology, a branched DNA assay is a signal amplification assay (as opposed to a target amplification assay) that is used to ... "A branched DNA signal amplification assay for quantification of nucleic acid targets below 100 molecules/ml". Nucleic Acids ... amplifier The assay can be used to detect and quantify many types of RNA or DNA target. In the assay, branched DNA is mixed ... Several different short single-stranded DNA molecules (oligonucleotides) are used in a branched DNA-assay. The capture and ...
https://en.wikipedia.org/wiki/Branched_DNA_assay
List of MeSH codes (E05)List of MeSH codes (E05)
... branched dna signal amplification assay MeSH E05.393.525.640 - oligonucleotide array sequence analysis MeSH E05.393.525.680 - ... branched dna signal amplification assay MeSH E05.393.661.250 - heteroduplex analysis MeSH E05.393.661.475 - in situ ... comet assay MeSH E05.393.560.598 - micronucleus tests MeSH E05.393.600.300 - dna footprinting MeSH E05.393.620.311 - ligase ... colony-forming units assay MeSH E05.200.500.383.910 - tumor stem cell assay MeSH E05.200.500.385 - cytogenetic analysis MeSH ...
https://en.wikipedia.org/wiki/List_of_MeSH_codes_(E05)
In situ hybridizationIn situ hybridization
An alternative technology, branched DNA assay, can be used for RNA (mRNA, lncRNA, and miRNA ) in situ hybridization assays with ... Separate but compatible signal amplification systems enable the multiplex assays. The signal can be visualized using a ... can be used to visualize up to four targets in one assay, and it uses patented probe design and bDNA signal amplification to ... A fully assembled signal amplification structure "Tree" has 400 binding sites for the label probes. When all target-specific ...
https://en.wikipedia.org/wiki/In_situ_hybridization
TransfectionTransfection
Graham FL, van der Eb AJ (April 1973). "A new technique for the assay of infectivity of human adenovirus 5 DNA". Virology. 52 ( ... Dendrimer is a class of highly branched molecules based on various building blocks and synthesized through a convergent or a ... Inhibiting interferon signaling disrupts the positive-feedback loop that normally hypersensitizes cells exposed to exogenous ... allow episomal amplification of plasmids containing the viral EBV (293E) or SV40 (293T) origins of replication, greatly ...
https://en.wikipedia.org/wiki/Transfection
Precision diagnosticsPrecision diagnostics
NOTCH signaling pathway, T-cell Receptor (TCR) signaling pathways, and T-cell associated genes (Tet2, Dmnt3) were found to be ... DNA sequencing is an essential component of modern scientific translational research and the use of DNA sequencing in the ... NGS offers a complete perspective in decoding the genome over any other single gene assays. NGS-based molecular diagnostics ... Precision diagnostics is a branch of precision medicine where a patient's healthcare model is precisely managed and specific ...
https://en.wikipedia.org/wiki/Precision_diagnostics
Viral loadViral load
To be comparable the same test method (target amplification, probe specific amplification, or signal amplification) should be ... One commonly used method: The branched DNA (bDNA) method can use either DNA or RNA as the target nucleic acid. Short probes ... When an assay for measuring the infective virus particle is done (Plaque assay, Focus assay), viral titre often refers to the ... The probes are then amplified Signal amplification uses large amounts of signal bound to an unamplified target originally ...
https://en.wikipedia.org/wiki/Viral_load
Schistosoma mansoniSchistosoma mansoni
Extracellular signal-regulated kinase and protein kinase C both respond to changes in medium temperature and light levels, and ... Loop-mediated isothermal amplification (LAMP) are being studied as they are lower cost. LAMP testing is not commercially ... The digestive tube is composed of an esophagus, which divides in two branches (right and left) and that reunite in a single ... Adult worm antigens can be detected by indirect haemagglutination assays (IHAs). Polymerase chain reaction (PCR) is also used ...
https://en.wikipedia.org/wiki/Schistosoma_mansoni
Rolling circle replicationRolling circle replication
It has also been used as an on-chip signal amplification method for nucleic acid (for both DNA and RNA) microarray assay. In ... Bacteriophage T4 DNA replication intermediates include circular and branched circular concatemeric structures. These structures ... and Vent exo-DNA polymerase for DNA amplification, and T7 RNA polymerase for RNA amplification. Since Phi29 DNA polymerase has ... This amplification technique is named as Rolling circle amplification (RCA). Different from conventional DNA amplification ...
https://en.wikipedia.org/wiki/Rolling_circle_replication
Synthetic biologySynthetic biology
1988: First DNA amplification by the polymerase chain reaction (PCR) using a thermostable DNA polymerase is published in ... It is widely employed in screening assays. Studies have considered the components of the DNA transcription mechanism. One ... such as responding to environmental signals, decision making and communication. Three key components are involved: DNA, RNA and ... It is a branch of science that encompasses a broad range of methodologies from various disciplines, such as biotechnology, ...
https://en.wikipedia.org/wiki/Synthetic_biology
TPX2TPX2
... at DNA double strand breaks and association with the machinery of DNA damage response that controls the amplification of γ-H2AX ... The rate of branched formation is also enhanced in the presence of both components compared to Ran alone. The TPX2 region ... Pfleger CM, Kirschner MW (March 2000). "The KEN box: an APC recognition signal distinct from the D box targeted by Cdh1". Genes ... in vitro ubiquitination assays have shown that only the first 83 amino acids of the N-terminal region of TPX2 along with the ...
https://en.wikipedia.org/wiki/TPX2
HIVHIV
Although much less commonly available, nucleic acid testing (e.g., viral RNA or proviral DNA amplification method) can also ... Although IFA can be used to confirm infection in these ambiguous cases, this assay is not widely used. In general, a second ... Nef's function in non-pathogenic forms of SIV is to downregulate expression of inflammatory cytokines, MHC-1, and signals that ... these subtypes form branches in the phylogenetic tree representing the lineage of the M group of HIV-1. Co-infection with ...
https://en.wikipedia.org/wiki/HIV
Molecular biologyMolecular biology
The transformed DNA gets attached to the DNA of E.coli and radioactivity is only seen onto the bacteriophage's DNA. This ... the Lowry procedure and the biuret assay. Unlike the previous methods, the Bradford assay is not susceptible to interference by ... Other branches of biology are informed by molecular biology, by either directly studying the interactions of molecules in their ... A variety of systems, such as inducible promoters and specific cell-signaling factors, are available to help express the ...
https://en.wikipedia.org/wiki/Molecular_biology
List of RNA-Seq bioinformatics toolsList of RNA-Seq bioinformatics tools
Recent sequencing technologies normally require DNA samples to be amplified via polymerase chain reaction (PCR). Amplification ... generating signal tracks of mapped reads, and segmenting that signal into actively transcribed regions. In addition to the ... DGEclust is a Python package for clustering expression data from RNA-seq, CAGE and other NGS assays using a Hierarchical ... Does not use any splice-site-finding heuristics optimized for a single taxonomic branch, but rather finds optimally-scoring ...
https://en.wikipedia.org/wiki/List_of_RNA-Seq_bioinformatics_tools
Hydrogen peroxideHydrogen peroxide
... is a signaling molecule of plant defense against pathogens. Hydrogen peroxide has roles as a signalling ... The amount of hydrogen peroxide in biological systems can be assayed using a fluorometric assay. About 60% of the world's ... It has been proposed that the enantiospecific interactions of one rather than the other may have led to amplification of one ... The toxicity is due to oxidation of proteins, membrane lipids and DNA by the peroxide ions. The class of biological enzymes ...
https://en.wikipedia.org/wiki/Hydrogen_peroxide
Single-cell analysisSingle-cell analysis
Once converted, there is not enough cDNA to be sequenced so the same DNA amplification techniques discussed in single cell ... Single-cell transcriptomic assays have allowed reconstruction development trajectories. Branching of these trajectories ... and to measure changes in protein expression and cell signaling in response to cancer treatment. Mass Cytometry: rare metal ... Antibody-DNA quantification: another antibody-based method converts protein levels to DNA levels. The conversion to DNA makes ...
https://en.wikipedia.org/wiki/Single-cell_analysis
Biosearch TechnologiesBiosearch Technologies
Cook's role in providing him one of the first SAM I DNA synthesizers which was used in support of Kary Mullis' PCR research. ... The series of Black Hole Quencher dyes have no native fluorescence, high signal-to-noise ratios providing greater sensitivity, ... RealTimeDesign is meant to help scientists craft custom oligonucleotides, averaging 99% in amplification efficiency through a ... Continually expanding their genomics portfolio offerings, ranging from high quality PCR reagents, custom genotyping assays, ...
https://en.wikipedia.org/wiki/Biosearch_Technologies
BRCA1BRCA1
... directly binds to DNA, with higher affinity for branched DNA structures. This ability to bind to DNA contributes to its ... BRCA1 combines with other tumor suppressors, DNA damage sensors and signal transducers to form a large multi-subunit protein ... Other methods have been proposed: traditional quantitative PCR, multiplex ligation-dependent probe amplification (MLPA), and ... "Real-time PCR-based gene dosage assay for detecting BRCA1 rearrangements in breast-ovarian cancer families". Clin. Genet. 65 (2 ...
https://en.wikipedia.org/wiki/BRCA1
Spinocerebellar ataxia type 1Spinocerebellar ataxia type 1
Ataxin 1 is involved in a number of signaling pathways and its expression is controlled by signaling pathways. The MAPK/ERK ... The process that turns coded information in DNA into proteins requires two steps: transcription, in which DNA is used to ... Amplification is done using polymerase chain reactions or PCR. The choice of primers can allow either for a single gene to be ... For repeat lengths within the range where interruptions are relevant, assays like CE and PAGE will not determine if the strain ...
https://en.wikipedia.org/wiki/Spinocerebellar_ataxia_type_1
Haplogroup T-M184Haplogroup T-M184
Y-DNA), and later Bronze Age individuals, all of whom belonged to Haplogroup J (Y-DNA). In the ancient city of Ebla in Syria in ... As a primary branch of haplogroup LT (a.k.a. K1), the basal, undivergent haplogroup T* currently has the alternate phylogenetic ... Plus amplification kit validation and calculation of forensic parameters for two Austrian populations". Forensic Science ... "Neolithic patrilineal signals indicate that the Armenian plateau was repopulated by agriculturalists". European Journal of ...
https://en.wikipedia.org/wiki/Haplogroup_T-M184
HerbicideHerbicide
Many diagnostic tests have been developed, including glasshouse pot assays, petri dish assays and chlorophyll fluorescence. A ... Target-site resistance may also be caused by an over-expression of the target enzyme (via gene amplification or changes in a ... Alberto, Diana; Serra, Anne-Antonella; Sulmon, Cécile; Gouesbet, Gwenola; Couée, Ivan (2016). "Herbicide-related signaling in ... is part of the first step in the synthesis of the branched-chain amino acids (valine, leucine, and isoleucine). These ...
https://en.wikipedia.org/wiki/Herbicide
GenomicsGenomics
Chain-termination methods require a single-stranded DNA template, a DNA primer, a DNA polymerase, normal ... A major branch of genomics is still concerned with sequencing the genomes of various organisms, but the knowledge of full ... In turn, proteins make up body structures such as organs and tissues as well as control chemical reactions and carry signals ... US 20050100900, Kawashima EH, Farinelli L, Mayer P, "Method of nucleic acid amplification", published 12 May 2005, issued 26 ...
https://en.wikipedia.org/wiki/Genomics
Glossary of genetics (M−Z)Glossary of genetics (M−Z)
See also single-stranded DNA and single-stranded RNA. single-stranded DNA (ssDNA) Any DNA molecule that consists of a single ... molecular genetics A branch of genetics that employs methods and techniques of molecular biology to study the structure and ... Amplification by PCR has become a standard technique in virtually all molecular biology laboratories. polymorphism The regular ... The first codon translated by a ribosome from a mature messenger RNA transcript, used as a signal to initiate {gli synthesis. ...
https://en.wikipedia.org/wiki/Glossary_of_genetics_(M−Z)
PhytoplasmaPhytoplasma
TENGU contains a signal peptide at its N-terminus; after cleavage, the mature protein is only 38 amino acids in length. ... In addition, loop-mediated isothermal amplification (a sensitive, simple, and rapid diagnostic method) is now available as a ... Like other prokaryotes, phytoplasmic DNA is distributed throughout the cytoplasm, instead of being concentrated in a nucleus.[ ... "Phytoplasma induced free-branching in commercial poinsettia cultivars". Nature Biotechnology. 15 (2): 178-182. doi:10.1038/ ...
https://en.wikipedia.org/wiki/Phytoplasma
William N. RomWilliam N. Rom
... aldehydes are the predominant forces inducing DNA damage and inhibiting DNA repair. Proceedings of the National Academy of ... He was a Senior Investigator at Pulmonary Branch, National Heart Lung and Blood Institute (NHLBI), NIH (1983-1989). William N. ... He pioneered nuclear acid amplification for the detection of tuberculosis in the sputum and blood. He introduced ... Identification of patients with active pulmonary tuberculosis using a peripheral blood-based polymerase chain reaction assay. ...
https://en.wikipedia.org/wiki/William_N._Rom
MTOR inhibitorsMTOR inhibitors
Signal transduction, Tor signaling pathway, Human proteins, Antineoplastic drugs, Oncology, Cancer treatments, Drug discovery) ... Thus, this data is based on preclinical assays, based on in vitro cultured tumor cell lines, which suggest that the effects of ... Research on mTOR inhibition has been a growing branch in science and has promising results. In general, protein kinases are ... Lovejoy CA, Cortez D (September 2009). "Common mechanisms of PIKK regulation". DNA Repair. 8 (9): 1004-1008. doi:10.1016/j. ...
https://en.wikipedia.org/wiki/MTOR_inhibitors
Glossary of geneticsGlossary of genetics
amplicon Any DNA or RNA sequence or fragment that is the source and/or product of an amplification reaction. The term is most ... 3. A step in some experimental assays in which a single-stranded DNA or RNA preparation is added to an array surface and ... Each of these triplets codes for a particular amino acid or stop signal during protein synthesis. DNA and RNA molecules are ... Cytosine bonded to deoxyribose is known as deoxycytidine, which is the version used in DNA. cytogenetics The branch of genetics ...
https://en.wikipedia.org/wiki/Glossary_of_genetics
Single-cell transcriptomicsSingle-cell transcriptomics
Secondly, bulk assays fail to recognize whether a change in the expression profile is due to a change in regulation or ... Wildsmith, S. E.; Archer, G. E.; Winkley, A. J.; Lane, P. W.; Bugelski, P. J. (1 January 2001). "Maximization of signal derived ... Fluorescent dyes are used as reporter molecules to detect the PCR product and monitor the progress of the amplification - the ... Another control uses unique molecular identifiers (UMIs)-short DNA sequences (6-10nt) that are added to each cDNA before ...
https://en.wikipedia.org/wiki/Single-cell_transcriptomics
Polymerase chain reactionPolymerase chain reaction
PCR allows isolation of DNA fragments from genomic DNA by selective amplification of a specific region of DNA. This use of PCR ... PCR assays can be performed directly on genomic DNA samples to detect translocation-specific malignant cells at a sensitivity ... so successful amplification with an SNP-specific primer signals presence of the specific SNP in a sequence. See SNP genotyping ... University of Texas Medical Branch at Galveston. ISBN 978-0-9631172-1-2. PMID 21413270. Yeh, Sylvia H.; Mink, ChrisAnna M. ( ...
https://en.wikipedia.org/wiki/Polymerase_chain_reaction
Glossary of genetics (0-L)Glossary of genetics (0-L)
amplicon Any DNA or RNA sequence or fragment that is the source and/or product of an amplification reaction. The term is most ... 3. A step in some experimental assays in which a single-stranded DNA or RNA preparation is added to an array surface and ... Each of these triplets codes for a particular amino acid or stop signal during protein synthesis. DNA and RNA molecules are ... Cytosine bonded to deoxyribose is known as deoxycytidine, which is the version used in DNA. cytogenetics The branch of genetics ...
https://en.wikipedia.org/wiki/Glossary_of_genetics_(0–L)
MicrofluidicsMicrofluidics
... glucose and lactate assays), DNA analysis (e.g., polymerase chain reaction and high-throughput sequencing), proteomics, and in ... level amplification enzymatic assay. Tumor materials can directly be used for detection through microfluidic devices. To screen ... However, the RPS method does not work well for particles below 1 μm diameter, as the signal-to-noise ratio falls below the ... Konda A, Morin SA (June 2017). "Flow-directed synthesis of spatially variant arrays of branched zinc oxide mesostructures". ...
https://en.wikipedia.org/wiki/Microfluidics
DeCS - Termos NovosDeCS - Termos Novos
ENSAIO DE AMPLIFICAÇÃO DE SINAL DE DNA RAMIFICADO. BRANCHED DNA SIGNAL AMPLIFICATION ASSAY. ENSAYO DE AMPLIFICACION DE SEÑAL DE ... DNA CATALÍTICO. DNA, CATALYTIC. ADN CATALITICO. DNA ESPAÇADOR RIBOSSÔMICO. DNA, RIBOSOMAL SPACER. ESPACIADOR RIBOSOMICO DE ADN ... DNA INTERGÊNICO. DNA, INTERGENIC. ADN INTERGENICO. DOENÇAS TRANSMISSÍVEIS EMERGENTES. COMMUNICABLE DISEASES, EMERGING. ... PROTEIN SORTING SIGNALS. SEÑALES DE CLASIFICACION DE PROTEINA. SÍNDROME DE HERMANSKI-PUDLAK. HERMANSKI-PUDLAK SYNDROME. ...
https://decs.bvs.br/P/news2001pie.htm
Report of the NIH Panel to Define Principles of Therapy of HIV InfectionReport of the NIH Panel to Define Principles of Therapy of HIV Infection
HIV-1 QT assay, Organon Teknika) or signal amplification methods (e.g., branched DNA {bDNA}, Quantiplex (TM) HIV RNA bDNA assay ... Rapid and precise quantification of HIV-1 RNA in plasma using a branched DNA signal amplification assay. J Acquir Immune Defic ... Clinical evaluation of branched DNA signal amplification for quantifying HIV type 1 in human plasma. AIDS Res Hum Retroviruses ... Target amplification assays are more sensitive (400 copies HIV RNA/mL plasma) than the first generation bDNA assay (10,000 ...
https://wonder.cdc.gov/wonder/prevguid/m0052295/m0052295.asp
Modeling, Prediction, and in Vitro in Vivo Correlation of CYP3A4 Induction | Drug Metabolism & DispositionModeling, Prediction, and in Vitro in Vivo Correlation of CYP3A4 Induction | Drug Metabolism & Disposition
mRNA Analysis. CYP3A4 mRNA content was determined with branched DNA (b-DNA) signal amplification technology using the Panomics ... Simple assays, such as reporter gene assays, that detect binding of inducers to receptors such as PXR have been used for ... b-DNA, branched-DNA; GADPH, glyceraldehyde-3-phosphate dehydrogenase; AUC, area under the curve; RSS, residual sum of squares; ... The receptor complex binds to the DNA-responsive element (DNARE) upstream of the CYP3A4 gene and activates its promoter, ...
https://dmd.aspetjournals.org/content/36/11/2355?ijkey=7e81fba1b8604b2b1ac84fec314bf437285a461d&keytype2=tf_ipsecsha
DeCS - New terms
BRANCHED DNA SIGNAL AMPLIFICATION ASSAY. ENSAYO DE AMPLIFICACION DE SEÑAL DE ADN RAMIFICADO. ENSAIO DE AMPLIFICAÇÃO DE SINAL DE ... DNA, INTERGENIC. ADN INTERGENICO. DNA INTERGÊNICO. DNA, RIBOSOMAL SPACER. ESPACIADOR RIBOSOMICO DE ADN. DNA ESPAÇADOR ... NUCLEIC ACID AMPLIFICATION TECHNIQUES. TECNICAS DE AMPLIFICACION DE ACIDO NUCLEICO. TÉCNICAS DE AMPLIFICAÇÃO DE ÁCIDO NUCLÉICO ... XENOGRAFT MODEL ANTITUMOR ASSAYS. ENSAYOS ANTITUMOR POR MODELO DE XENOINJERTO. ENSAIOS ANTITUMORAIS MODELO DE XENOENXERTO. ...
https://decs2013.bvsalud.org/I/news2001iep.htm
DeCS - New terms
BRANCHED DNA SIGNAL AMPLIFICATION ASSAY. ENSAYO DE AMPLIFICACION DE SEÑAL DE ADN RAMIFICADO. ENSAIO DE AMPLIFICAÇÃO DE SINAL DE ... DNA, INTERGENIC. ADN INTERGENICO. DNA INTERGÊNICO. DNA, RIBOSOMAL SPACER. ESPACIADOR RIBOSOMICO DE ADN. DNA ESPAÇADOR ... NUCLEIC ACID AMPLIFICATION TECHNIQUES. TECNICAS DE AMPLIFICACION DE ACIDO NUCLEICO. TÉCNICAS DE AMPLIFICAÇÃO DE ÁCIDO NUCLÉICO ... XENOGRAFT MODEL ANTITUMOR ASSAYS. ENSAYOS ANTITUMOR POR MODELO DE XENOINJERTO. ENSAIOS ANTITUMORAIS MODELO DE XENOENXERTO. ...
https://decs2014.bvsalud.org/I/news2001iep.htm
DeCS - New termsDeCS - New terms
BRANCHED DNA SIGNAL AMPLIFICATION ASSAY. ENSAYO DE AMPLIFICACION DE SEÑAL DE ADN RAMIFICADO. ENSAIO DE AMPLIFICAÇÃO DE SINAL DE ... DNA, INTERGENIC. ADN INTERGENICO. DNA INTERGÊNICO. DNA, RIBOSOMAL SPACER. ESPACIADOR RIBOSOMICO DE ADN. DNA ESPAÇADOR ... NUCLEIC ACID AMPLIFICATION TECHNIQUES. TECNICAS DE AMPLIFICACION DE ACIDO NUCLEICO. TÉCNICAS DE AMPLIFICAÇÃO DE ÁCIDO NUCLÉICO ... XENOGRAFT MODEL ANTITUMOR ASSAYS. ENSAYOS ANTITUMOR POR MODELO DE XENOINJERTO. ENSAIOS ANTITUMORAIS MODELO DE XENOENXERTO. ...
https://decs.bvsalud.org/I/news2001iep.htm
DeCS - New terms
BRANCHED DNA SIGNAL AMPLIFICATION ASSAY. ENSAYO DE AMPLIFICACION DE SEÑAL DE ADN RAMIFICADO. ENSAIO DE AMPLIFICAÇÃO DE SINAL DE ... DNA, INTERGENIC. ADN INTERGENICO. DNA INTERGÊNICO. DNA, RIBOSOMAL SPACER. ESPACIADOR RIBOSOMICO DE ADN. DNA ESPAÇADOR ... NUCLEIC ACID AMPLIFICATION TECHNIQUES. TECNICAS DE AMPLIFICACION DE ACIDO NUCLEICO. TÉCNICAS DE AMPLIFICAÇÃO DE ÁCIDO NUCLÉICO ... XENOGRAFT MODEL ANTITUMOR ASSAYS. ENSAYOS ANTITUMOR POR MODELO DE XENOINJERTO. ENSAIOS ANTITUMORAIS MODELO DE XENOENXERTO. ...
https://decs2012.bvsalud.org/I/news2001iep.htm
DeCS - New terms
BRANCHED DNA SIGNAL AMPLIFICATION ASSAY. ENSAYO DE AMPLIFICACION DE SEÑAL DE ADN RAMIFICADO. ENSAIO DE AMPLIFICAÇÃO DE SINAL DE ... DNA, INTERGENIC. ADN INTERGENICO. DNA INTERGÊNICO. DNA, RIBOSOMAL SPACER. ESPACIADOR RIBOSOMICO DE ADN. DNA ESPAÇADOR ... NUCLEIC ACID AMPLIFICATION TECHNIQUES. TECNICAS DE AMPLIFICACION DE ACIDO NUCLEICO. TÉCNICAS DE AMPLIFICAÇÃO DE ÁCIDO NUCLÉICO ... XENOGRAFT MODEL ANTITUMOR ASSAYS. ENSAYOS ANTITUMOR POR MODELO DE XENOINJERTO. ENSAIOS ANTITUMORAIS MODELO DE XENOENXERTO. ...
https://decs2008.bvsalud.org/I/news2001iep.htm
DeCS - New terms
BRANCHED DNA SIGNAL AMPLIFICATION ASSAY. ENSAYO DE AMPLIFICACION DE SEÑAL DE ADN RAMIFICADO. ENSAIO DE AMPLIFICAÇÃO DE SINAL DE ... DNA, INTERGENIC. ADN INTERGENICO. DNA INTERGÊNICO. DNA, RIBOSOMAL SPACER. ESPACIADOR RIBOSOMICO DE ADN. DNA ESPAÇADOR ... NUCLEIC ACID AMPLIFICATION TECHNIQUES. TECNICAS DE AMPLIFICACION DE ACIDO NUCLEICO. TÉCNICAS DE AMPLIFICAÇÃO DE ÁCIDO NUCLÉICO ... XENOGRAFT MODEL ANTITUMOR ASSAYS. ENSAYOS ANTITUMOR POR MODELO DE XENOINJERTO. ENSAIOS ANTITUMORAIS MODELO DE XENOENXERTO. ...
https://decs2018.bvsalud.org/I/news2001iep.htm
DeCS - New terms
BRANCHED DNA SIGNAL AMPLIFICATION ASSAY. ENSAYO DE AMPLIFICACION DE SEÑAL DE ADN RAMIFICADO. ENSAIO DE AMPLIFICAÇÃO DE SINAL DE ... DNA, INTERGENIC. ADN INTERGENICO. DNA INTERGÊNICO. DNA, RIBOSOMAL SPACER. ESPACIADOR RIBOSOMICO DE ADN. DNA ESPAÇADOR ... NUCLEIC ACID AMPLIFICATION TECHNIQUES. TECNICAS DE AMPLIFICACION DE ACIDO NUCLEICO. TÉCNICAS DE AMPLIFICAÇÃO DE ÁCIDO NUCLÉICO ... XENOGRAFT MODEL ANTITUMOR ASSAYS. ENSAYOS ANTITUMOR POR MODELO DE XENOINJERTO. ENSAIOS ANTITUMORAIS MODELO DE XENOENXERTO. ...
https://decs2010.bvsalud.org/I/news2001iep.htm
DeCS - New terms
BRANCHED DNA SIGNAL AMPLIFICATION ASSAY. ENSAYO DE AMPLIFICACION DE SEÑAL DE ADN RAMIFICADO. ENSAIO DE AMPLIFICAÇÃO DE SINAL DE ... DNA, INTERGENIC. ADN INTERGENICO. DNA INTERGÊNICO. DNA, RIBOSOMAL SPACER. ESPACIADOR RIBOSOMICO DE ADN. DNA ESPAÇADOR ... NUCLEIC ACID AMPLIFICATION TECHNIQUES. TECNICAS DE AMPLIFICACION DE ACIDO NUCLEICO. TÉCNICAS DE AMPLIFICAÇÃO DE ÁCIDO NUCLÉICO ... XENOGRAFT MODEL ANTITUMOR ASSAYS. ENSAYOS ANTITUMOR POR MODELO DE XENOINJERTO. ENSAIOS ANTITUMORAIS MODELO DE XENOENXERTO. ...
https://decs2015.bvsalud.org/I/news2001iep.htm
DeCS - New terms
BRANCHED DNA SIGNAL AMPLIFICATION ASSAY. ENSAYO DE AMPLIFICACION DE SEÑAL DE ADN RAMIFICADO. ENSAIO DE AMPLIFICAÇÃO DE SINAL DE ... DNA, INTERGENIC. ADN INTERGENICO. DNA INTERGÊNICO. DNA, RIBOSOMAL SPACER. ESPACIADOR RIBOSOMICO DE ADN. DNA ESPAÇADOR ... NUCLEIC ACID AMPLIFICATION TECHNIQUES. TECNICAS DE AMPLIFICACION DE ACIDO NUCLEICO. TÉCNICAS DE AMPLIFICAÇÃO DE ÁCIDO NUCLÉICO ... XENOGRAFT MODEL ANTITUMOR ASSAYS. ENSAYOS ANTITUMOR POR MODELO DE XENOINJERTO. ENSAIOS ANTITUMORAIS MODELO DE XENOENXERTO. ...
https://decs2020.bvsalud.org/I/news2001iep.htm
DeCS - New terms
BRANCHED DNA SIGNAL AMPLIFICATION ASSAY. ENSAYO DE AMPLIFICACION DE SEÑAL DE ADN RAMIFICADO. ENSAIO DE AMPLIFICAÇÃO DE SINAL DE ... DNA, INTERGENIC. ADN INTERGENICO. DNA INTERGÊNICO. DNA, RIBOSOMAL SPACER. ESPACIADOR RIBOSOMICO DE ADN. DNA ESPAÇADOR ... NUCLEIC ACID AMPLIFICATION TECHNIQUES. TECNICAS DE AMPLIFICACION DE ACIDO NUCLEICO. TÉCNICAS DE AMPLIFICAÇÃO DE ÁCIDO NUCLÉICO ... XENOGRAFT MODEL ANTITUMOR ASSAYS. ENSAYOS ANTITUMOR POR MODELO DE XENOINJERTO. ENSAIOS ANTITUMORAIS MODELO DE XENOENXERTO. ...
https://decs2017.bvsalud.org/I/news2001iep.htm
DeCS - New terms
BRANCHED DNA SIGNAL AMPLIFICATION ASSAY. ENSAYO DE AMPLIFICACION DE SEÑAL DE ADN RAMIFICADO. ENSAIO DE AMPLIFICAÇÃO DE SINAL DE ... DNA, INTERGENIC. ADN INTERGENICO. DNA INTERGÊNICO. DNA, RIBOSOMAL SPACER. ESPACIADOR RIBOSOMICO DE ADN. DNA ESPAÇADOR ... NUCLEIC ACID AMPLIFICATION TECHNIQUES. TECNICAS DE AMPLIFICACION DE ACIDO NUCLEICO. TÉCNICAS DE AMPLIFICAÇÃO DE ÁCIDO NUCLÉICO ... XENOGRAFT MODEL ANTITUMOR ASSAYS. ENSAYOS ANTITUMOR POR MODELO DE XENOINJERTO. ENSAIOS ANTITUMORAIS MODELO DE XENOENXERTO. ...
https://decs2007.bvsalud.org/I/news2001iep.htm
DeCS - New terms
BRANCHED DNA SIGNAL AMPLIFICATION ASSAY. ENSAYO DE AMPLIFICACION DE SEÑAL DE ADN RAMIFICADO. ENSAIO DE AMPLIFICAÇÃO DE SINAL DE ... DNA, INTERGENIC. ADN INTERGENICO. DNA INTERGÊNICO. DNA, RIBOSOMAL SPACER. ESPACIADOR RIBOSOMICO DE ADN. DNA ESPAÇADOR ... NUCLEIC ACID AMPLIFICATION TECHNIQUES. TECNICAS DE AMPLIFICACION DE ACIDO NUCLEICO. TÉCNICAS DE AMPLIFICAÇÃO DE ÁCIDO NUCLÉICO ... XENOGRAFT MODEL ANTITUMOR ASSAYS. ENSAYOS ANTITUMOR POR MODELO DE XENOINJERTO. ENSAIOS ANTITUMORAIS MODELO DE XENOENXERTO. ...
https://decs2011.bvsalud.org/I/news2001iep.htm
DeCS - New terms
BRANCHED DNA SIGNAL AMPLIFICATION ASSAY. ENSAYO DE AMPLIFICACION DE SEÑAL DE ADN RAMIFICADO. ENSAIO DE AMPLIFICAÇÃO DE SINAL DE ... DNA, INTERGENIC. ADN INTERGENICO. DNA INTERGÊNICO. DNA, RIBOSOMAL SPACER. ESPACIADOR RIBOSOMICO DE ADN. DNA ESPAÇADOR ... NUCLEIC ACID AMPLIFICATION TECHNIQUES. TECNICAS DE AMPLIFICACION DE ACIDO NUCLEICO. TÉCNICAS DE AMPLIFICAÇÃO DE ÁCIDO NUCLÉICO ... XENOGRAFT MODEL ANTITUMOR ASSAYS. ENSAYOS ANTITUMOR POR MODELO DE XENOINJERTO. ENSAIOS ANTITUMORAIS MODELO DE XENOENXERTO. ...
https://decs2019.bvsalud.org/I/news2001iep.htm
DeCS - New terms
BRANCHED DNA SIGNAL AMPLIFICATION ASSAY. ENSAYO DE AMPLIFICACION DE SEÑAL DE ADN RAMIFICADO. ENSAIO DE AMPLIFICAÇÃO DE SINAL DE ... DNA, INTERGENIC. ADN INTERGENICO. DNA INTERGÊNICO. DNA, RIBOSOMAL SPACER. ESPACIADOR RIBOSOMICO DE ADN. DNA ESPAÇADOR ... NUCLEIC ACID AMPLIFICATION TECHNIQUES. TECNICAS DE AMPLIFICACION DE ACIDO NUCLEICO. TÉCNICAS DE AMPLIFICAÇÃO DE ÁCIDO NUCLÉICO ... XENOGRAFT MODEL ANTITUMOR ASSAYS. ENSAYOS ANTITUMOR POR MODELO DE XENOINJERTO. ENSAIOS ANTITUMORAIS MODELO DE XENOENXERTO. ...
https://decs2016.bvsalud.org/I/news2001iep.htm
DeCS - New terms
BRANCHED DNA SIGNAL AMPLIFICATION ASSAY. ENSAYO DE AMPLIFICACION DE SEÑAL DE ADN RAMIFICADO. ENSAIO DE AMPLIFICAÇÃO DE SINAL DE ... DNA, INTERGENIC. ADN INTERGENICO. DNA INTERGÊNICO. DNA, RIBOSOMAL SPACER. ESPACIADOR RIBOSOMICO DE ADN. DNA ESPAÇADOR ... NUCLEIC ACID AMPLIFICATION TECHNIQUES. TECNICAS DE AMPLIFICACION DE ACIDO NUCLEICO. TÉCNICAS DE AMPLIFICAÇÃO DE ÁCIDO NUCLÉICO ... XENOGRAFT MODEL ANTITUMOR ASSAYS. ENSAYOS ANTITUMOR POR MODELO DE XENOINJERTO. ENSAIOS ANTITUMORAIS MODELO DE XENOENXERTO. ...
https://decs2009.bvsalud.org/I/news2001iep.htm
Circovirus Hepatitis Infection in Heart-Lung Transplant Patient, France - Volume 29, Number 2-February 2023 - Emerging...
... which were revealed by a red signal (probe type 1 in the assay kit); a blue signal (probe type 6 in the kit) revealed a mix of ... We designed a cocktail of 12 custom-branched DNA probes to target HCirV-1 capsid genes and 20 probes to target Rep genes, ... and EBV DNA load using the bioMérieux EBV R-GENE kit. We performed real-time PCR amplification on an Applied Biosystems AB7500 ... We designed an assay and conducted qPCR to assess changes in the viral load in plasma and other biologic samples taken from the ...
https://wwwnc.cdc.gov/eid/article/29/2/22-1468
Circovirus Hepatitis Infection in Heart-Lung Transplant Patient, France - Volume 29, Number 2-February 2023 - Emerging...
... which were revealed by a red signal (probe type 1 in the assay kit); a blue signal (probe type 6 in the kit) revealed a mix of ... We designed a cocktail of 12 custom-branched DNA probes to target HCirV-1 capsid genes and 20 probes to target Rep genes, ... and EBV DNA load using the bioMérieux EBV R-GENE kit. We performed real-time PCR amplification on an Applied Biosystems AB7500 ... We designed an assay and conducted qPCR to assess changes in the viral load in plasma and other biologic samples taken from the ...
https://wwwnc.cdc.gov/eid/article/29/2/22-1468_article
Honyaku Star - Search: 幅Honyaku Star - Search: 幅
branched DNA signal amplification assay. *ランダム増幅多型DNA法. *random amplified polymorphic DNA technique ...
http://honyakustar.com/jp/%E5%B9%85
Prognostic significance of the mRNA expression of ERCC1, RRM1, TUBB3 and TYMS genes in patients with non-small cell lung cancerPrognostic significance of the mRNA expression of ERCC1, RRM1, TUBB3 and TYMS genes in patients with non-small cell lung cancer
Flagella M, Bui S, Zheng Z, et al: A multiplex branched DNA assay for parallel quantitative gene expression profiling. Anal ... then coupled with a fluorescence signal amplification system (8,9). The signals were detected using the Luminex® 200 system ( ... TUBB3 and TYMS using multiplex branched-DNA liquidchip technology. Multiplex branched-DNA liquidchip technology measures mRNA ... Signal amplification steps were performed through incubation with 100 µl pre-amplifier solution for 1 h at 50°C, followed by ...
https://www.spandidos-publications.com/10.3892/etm.2015.2636?text=fulltext
Cytomegalovirus (CMV) Retinitis Treatment & Management: Medical Care, Surgical Care, ConsultationsCytomegalovirus (CMV) Retinitis Treatment & Management: Medical Care, Surgical Care, Consultations
... is a ubiquitous DNA virus that infects the majority of the adult population. In the immunocompetent host, infection is ... Quantification of cytomegalovirus DNA in peripheral blood leukocytes by a branched-DNA signal amplification assay. J Clin ... Comparison of three assays for cytomegalovirus detection in AIDS patients at risk for retinitis. J Clin Microbiol. 2000 Feb. 38 ... Cytomegalovirus (CMV) blood DNA load, CMV retinitis progression, and occurrence of resistant CMV in patients with CMV retinitis ...
https://emedicine.staging.medscape.com/article/1227228-treatment
Hepatitis C Workup: Approach Considerations, Hepatitis C Antibody Test, Qualitative and Quantitative Assays for HCV RNA
Quantitative assays. Quantitative assays ascertain HCV RNA quantity in blood, using signal amplification (branched DNA [bDNA] ... Qualitative and Quantitative Assays for HCV RNA. Qualitative assays. Qualitative assays can be used to test for hepatitis C ... Versant HCV Genotyping Assay (INNO-LiPA) 2.0: Next-generation line-probe assay ... assay) or target amplification techniques (PCR, TMA). RT-PCR is more sensitive than bDNA testing. The HCV RNA level in blood ...
https://www.staging.medscape.com/answers/177792-3849/what-is-the-role-of-genotyping-in-the-workup-of-hepatitis-c-hep-c-infection
HIV Testing Overview: Overview, HIV Virion and Genome, HIV Testing RecommendationsHIV Testing Overview: Overview, HIV Virion and Genome, HIV Testing Recommendations
... and signal amplification (branched-chain DNA). [22] These assays are used for screening of blood products, therapeutic ... HIV-1 Nucleic Acid Amplification Techniques (NAATs). The detection of the proviral DNA is rarely used in clinical practice, the ... 22] has approved a qualitative transcription-mediated amplification/hybridization protection assay (TMA/HPA) [47] for the ... Screening Assays. The available screening assays rely on the detection of antibodies, the p24 antigen, or the viral nucleic ...
https://emedicine.staging.medscape.com/article/2061077-overview
MeSH BrowserMeSH Browser
Branched DNA Signal Amplification Assay [E05.601.300] * Immunoassay [E05.601.470] * Molecular Imaging [E05.601.555] * Single ...
https://meshb.nlm.nih.gov/record/ui?ui=D057054
Michael J. Powell | DiaCarta, Inc , USA | Biotechnology Summit 2021 | Allied AcademiesMichael J. Powell | DiaCarta, Inc , USA | Biotechnology Summit 2021 | Allied Academies
... most notably the development of branched DNA (bDNA) signal amplification and a novel somatic gene mutation Real-Time PCR based ... He was a pioneer and lead scientist and inventor of the electrochemiluminescence (ECL) assay technology and also developed ... a privately held company that commercialized a proprietary fluorescent live cell-based assay and diagnostic imaging technology ... assay technology called QClampTM for applications in the diagnosis of cancer and infectious diseases and the rapid detection of ...
https://biotechnology.alliedacademies.com/ocm/2021/michael-j-powell-diacarta-inc-usa
MeSH BrowserMeSH Browser
Branched DNA Signal Amplification Assay [E05.601.300] * Immunoassay [E05.601.470] * Molecular Imaging [E05.601.555] ... Random Amplified Polymorphic DNA Technic Previous Indexing. Polymerase Chain Reaction (1991-1995). See Also. DNA Fingerprinting ... Random Amplified Polymorphic DNA Technique Preferred Term Term UI T057049. Date01/01/1999. LexicalTag ACX. ThesaurusID NLM ( ... amplification with single primers of arbitrary sequence to generate strain-specific arrays of anonymous DNA fragments. RAPD ...
https://meshb-prev.nlm.nih.gov/record/ui?ui=D019105
Circovirus Hepatitis Infection in Heart-Lung Transplant Patient, France - Volume 29, Number 2-February 2023 - Emerging...
... which were revealed by a red signal (probe type 1 in the assay kit); a blue signal (probe type 6 in the kit) revealed a mix of ... We designed a cocktail of 12 custom-branched DNA probes to target HCirV-1 capsid genes and 20 probes to target Rep genes, ... and EBV DNA load using the bioMérieux EBV R-GENE kit. We performed real-time PCR amplification on an Applied Biosystems AB7500 ... We designed an assay and conducted qPCR to assess changes in the viral load in plasma and other biologic samples taken from the ...
https://wwwnc-origin.cdc.gov/eid/article/29/2/22-1468
PrimersNucleic acid hybridizPathwaysImmunoblot assayDiagnosticFluorescencePolymeraseSequencesGeneAlkaline phosphataseGenomeTumorEnzymeHybridizationDetectionProbesNUCLEASESAnalysesProliferationProteinsMolecularProteinFluorescentGenesLAMPPathwaySynthesisReceptorsRestrictionDouble strandedProbeRAPDExpressionDuplexMethylationRolesAntibodiesClinicalIncorporationDonorInvolvesHostInfectionSequencingEMERGINGPresentDetectBreastCellSpecificDomainControlBlood
Primers4
  • Technique that utilizes low-stringency polymerase chain reaction ( PCR ) amplification with single primers of arbitrary sequence to generate strain-specific arrays of anonymous DNA fragments. (nih.gov)
  • It also includes biotinylated β-globin primers as an internal control for sample amplification. (cdc.gov)
  • Three pairs of amplification primers required for this method were derived from a conserved DNA sequence unique to the P. vivax genome. (cdc.gov)
  • Primers for new LAMP assay (named as DS-LAMP) were designed from a histidine-tRNA ligase gene (EAMY_RS32025) of E. amylovora CFBP1430 genome. (ppjonline.org)
Nucleic acid hybridiz2
  • The multiplex branched-DNA technology is a sandwich nucleic acid hybridization method in which targets are captured through cooperative hybridization of multiple probes, then coupled with a fluorescence signal amplification system ( 8 , 9 ). (spandidos-publications.com)
  • This test, using Hybrid Capture 2 technology, is a nucleic acid hybridization microplate assay with signal amplification. (cdc.gov)
Pathways4
  • Development of activity-based probes for ubiquitin and ubiquitin-like protein signaling pathways. (viictr.org)
  • While the PI3 kinase signalling regulated pathways and genes have been comprehensively studied, its impact on the miRNome is yet to be explored. (biomedcentral.com)
  • Annexin V and TUNEL were used together with Western blotting to investigate the signaling pathways. (biomedcentral.com)
  • Biological DNA Sensor defines the meaning of DNA sensing pathways and demonstrates the importance of the innate immune responses induced by double stranded DNA (dsDNA) through its influencing functions in disease pathology and immune activity of adjuvants for vaccines. (mewsie.org)
Immunoblot assay2
  • Recombinant immunoblot assay. (cdc.gov)
  • The recombinant immunoblot assay is used to confirm HCV infection. (medscape.com)
Diagnostic5
  • Mike was previously a Founder of Odyssey Thera Inc., a privately held company that commercialized a proprietary fluorescent live cell-based assay and diagnostic imaging technology for the application in target validation and drug discovery. (alliedacademies.com)
  • The coding region of the UFGT gene is a source of diagnostic SNP markers that allow single-locus DNA genotyping for the assessment of cultivar identity and ancestry in grapevine (Vitis vinifera L. (biomedcentral.com)
  • The Institute for Clinical and Experimental Research ® is focused on the development of new diagnostic assays in a wide range of disciplines. (aruplab.com)
  • Loop-mediated isothermal amplification (LAMP) is known as the best on-site diagnostic, because it is fast, highly specific to a target, and less sensitive to inhibitors in samples. (ppjonline.org)
  • Molecular Diagnostic and Prognostication Assays for the Subtyping of Urinary Bladder Cancer Are on the Way to Illuminating Our Vision. (who.int)
Fluorescence6
  • Determination of HER2/neu status: a pilot study comparing HER2/neu dual in situ hybridization DNA probe cocktail assay performed on cell blocks to immunohistochemisty and fluorescence in situ hybridization performed on histologic specimens. (viictr.org)
  • Known fluorescence polymerase chain reaction (PCR) monitoring techniques include both strand specific and generic DNA intercalator techniques that can be used on a few second-generation PCR thermal cycling devices. (allindianpatents.com)
  • The precise form of the assays varies but often relies on fluorescence energy transfer or FET between two fluorescent moieties within the system in order to generate a signal indicative o-f the presence of the product of amplification. (allindianpatents.com)
  • The probe comprises a reactive molecule able to absorb fluorescence from or donate fluorescent energy to said DNA duplex binding agent. (allindianpatents.com)
  • Thus the signal changes in fluorescence from the reactive molecule, which are indicative of the formation or destabilisation of duplexes involving the probe, are preferably monitored. (allindianpatents.com)
  • The amplification was carried out at 64°C using SYBR Green or SYTO-9 intercalating dyes for 90 minutes with the tube scanner set to collect fluorescence signals at 1-minute intervals. (cdc.gov)
Polymerase2
  • This assay uses Roche Linear Array HPV Genotyping test that is based on HPV L1 consensus polymerase chain reaction (PCR) with biotinylated PGMY09/11 primer sets. (cdc.gov)
  • We analyzed the internal transcribed spacer 2 (ITS2) region of Biomphalaria ribosomal DNA (rDNA) using polymerase chain reaction amplification (PCR) and restriction fragment length polymorphism (RFLP). (biomedcentral.com)
Sequences7
  • Test to detect HCV RNA by amplification of viral genetic sequences. (cdc.gov)
  • Tests to detect HCV RNA concentration (viral load) by amplification of viral genetic sequences or by signal amplification. (cdc.gov)
  • The analysis of the chloroplast target regions proved the inadequacy of the DNA barcoding approach at the subspecies level, and hence further DNA genotyping analyses were targeted on the sequences of five nuclear single-copy genes amplified across all of the accessions. (biomedcentral.com)
  • An RLU measurement equal to or greater than the Cutoff Value indicates the presence of HPV DNA sequences in the specimen. (cdc.gov)
  • An RLU measurement less than the Cutoff Value indicates the absence of the specific HPV DNA sequences tested or HPV DNA levels below the detection limit of the assay. (cdc.gov)
  • Coupled with these devices have been a number of novel signal transducers, incorporation of modified nucleic acid capture sequences, and the development of new signal amplification strategies. (genalyte.com)
  • In these examples, multiplexing must typically be achieved spectrally, which places some constraints on the number of sequences that can be simultaneously assayed. (genalyte.com)
Gene4
  • The receptor complex binds to the DNA-responsive element (DNA RE ) upstream of the CYP3A4 gene and activates its promoter, leading to transcription of the CYP3A4 gene. (aspetjournals.org)
  • Variants were subjected to DNA-protein interaction analyses (electrophoretic mobility shift assay), reporter gene assays in breast cancer cell lines MDA134 and MDA157, and immunohistochemical analyses of breast tumors. (aacrjournals.org)
  • KEY MESSAGE: Overexpression of the tea plant gene CsbZIP18 in Arabidopsis impaired freezing tolerance, and CsbZIP18 is a negative regulator of ABA signaling and cold stress. (usda.gov)
  • Further experiments demonstrated that resveratrol had little effect on VACV early gene expression, while it suppressed VACV DNA synthesis, and subsequently post-replicative gene expression. (researchgate.net)
Alkaline phosphatase1
  • Immobilized hybrids are then reacted with alkaline phosphatase conjugated antibodies specific for the RNA: DNA hybrids, and detected with a chemiluminescent substrate. (cdc.gov)
Genome4
  • Dames S, Eilbeck K, Mao R A high-throughput next-generation sequencing assay for the mitochondrial genome. (aruplab.com)
  • The presence of genome-wide DNA hypermethylation is a hallmark of lower grade gliomas (LGG) with isocitrate dehydrogenase (IDH) mutations. (biomedcentral.com)
  • Limouse C, Jukam D, Smith OK, Fryer KA and Straight AF, 2020, Mapping Transcriptome-Wide and Genome-Wide RNA-DNA Contacts with Chromatin-Associated RNA Sequencing (ChAR-seq). (stanford.edu)
  • Selective Whole-Genome Amplification as a Tool to Enrich Specimens with Low Treponema pallidum Genomic DNA Copies for Whole-Genome Sequencing. (cdc.gov)
Tumor3
  • The mRNA expression of these genes was assessed in 72 tumor tissue samples obtained following surgery, using multiplex branched‑DNA technology. (spandidos-publications.com)
  • In the present study, multiplex branched-DNA liquidchip technology was used to analyze the mRNA expression of the ERCC1, RRM1, TUBB3 and TYMS genes in tumor tissue samples from patients with resected NSCLC prior to chemotherapy. (spandidos-publications.com)
  • PR is required for tumor growth in patient samples and sufficient to drive tumor growth and metastasis in ER signaling ablated tumor cells. (icr.ac.uk)
Enzyme6
  • The ribonucleoside-diphosphate reductase enzyme is essential for the production of deoxyribonucleotides prior to DNA synthesis. (spandidos-publications.com)
  • Thymidylate synthase (TYMS) is the enzyme used to generate thymidine monophosphate (dTMP), which is subsequently phosphorylated to thymidine triphosphate for use in DNA synthesis and repair. (spandidos-publications.com)
  • However, in clinical practice, the most common method for diagnosing established HIV infection is by performing a screening test (eg, enzyme-linked immunosorbent assay [ELISA]) and by confirming a positive result with a supplementary test. (medscape.com)
  • Electrochemiluminescent assays have also been developed that offer promising enzyme- and nucleic acid-based signal enhancement strategies. (genalyte.com)
  • Isoform switching in trout gills following salinity transfer suggests that the Na + /K + -ATPase α1a- andα1b-isoforms play different roles in freshwater and seawater acclimation, and that assays of Na + /K + -ATPase enzyme activity may not provide a complete picture of the role of this protein in seawater transfer. (biologists.com)
  • The amplification of the ITS2 region of Biomphalaria snails resulted in a 490 bp fragment and produced two profiles for each species after digestion with the restriction enzyme Hpa II. (biomedcentral.com)
Hybridization1
  • After amplification the samples are typed by hybridization to the typing strips followed by colorimetric detection. (cdc.gov)
Detection6
  • Zebra Tail Amplification: Accelerated Detection of Apoptotic Blunt-Ended DNA Breaks by In Situ Ligation. (viictr.org)
  • Detection and typing of HPV DNA in vaginal swabs (in conjunction with testing of NHANES sera for HPV antibody) will allow evaluation of trends in prevalence of type-specific HPV infection by age, sexual behavior, and race/ethnicity. (cdc.gov)
  • It uses chemiluminescence for the qualitative detection of eighteen types of human papillomavirus (HPV) DNA in cervical specimens. (cdc.gov)
  • To improve upon microarrays, which most commonly require labeling of the miRNA before detection, fluorescent assays today aim to use fluorescent reporters that bind to the miRNA target via a detection probe. (genalyte.com)
  • Our laboratory has recently developed a new platform called RealAmp, which combines loop-mediated isothermal amplification (LAMP) with a portable tube scanner real-time isothermal instrument for the rapid detection of malaria parasites. (cdc.gov)
  • The DS-LAMP amplified DNA (positive detection) only from genomic DNA of E. amylovora strains, not from either E. pyrifoliae (causing black shoot blight) or from Pseudomonas syringae pv. (ppjonline.org)
Probes2
  • A simple, but effective, FRET approach used a DNA strand-displacement scheme, where fluorescently tagged DNA capture probes were initially hybridized with a quencher functionalized compliment. (genalyte.com)
  • The use of a DNA duplex binding agent such as an intercalating dye and a probe which is singly labelled is advantageous in that these components are much more economical than other assays in which doubly labelled probes are required. (allindianpatents.com)
NUCLEASES1
  • Three nucleases involved in DNA end resection, MRE11, EXO1, and DNA2 are responsible for the degradation of nascent DNA strands. (nature.com)
Analyses1
  • Phylogenetic analyses showed that ArGR formed a distinct branch separate from the teleosts GRs. (biomedcentral.com)
Proliferation1
  • Proliferation assay, colony suppression assay and wound healing assay were carried out in pre-miR transfected cells to investigate its role in malignant transformation. (biomedcentral.com)
Proteins2
  • C) RNA immunoprecipitation assayed by real-time RT-PCR of wildtype and G1G2 mutant HRAS intron 5 RNA bound by hnRNP H and hnRNP F proteins. (bioz.com)
  • In S. cerevisiae it has been shown that, Alpelisib purchase using a two-hybrid assay, the four homologous proteins physically interact. (nartpathway.com)
Molecular4
  • A molecular inversion probe assay for detecting alternative splicing. (viictr.org)
  • Multigene assays for molecular subtypes and biomarkers can aid management of early invasive breast cancer. (icr.ac.uk)
  • Open chromatin assay points to differential accessibility of non-coding RNAs as an important source of epigenetic heterogeneity within individual tumors and between molecular subgroups. (biomedcentral.com)
  • Gliomas with IDH mutation exhibit global DNA hypermethylation and are subdivided into two distinct molecular subgroups: IDHmut-codel (hemizygous co-deletion of chromosome arms 1p/19q) and IDHmut-noncodel (without co-deletion of 1p/19q) gliomas [ 4 ]. (biomedcentral.com)
Protein6
  • The central HR protein, RAD51, forms helical nucleoprotein filaments on tracts of single-strand DNA (ssDNA), such as those formed by nucleolytic processing of the DNA ends at the sites of DNA double strand breaks (DSBs). (nature.com)
  • Once RAD51 filaments form on tracts of ssDNA, the protein alters the structure of the ssDNA, allowing the nucleoprotein filament to catalyze a homology search to identify an identical or nearly identical sequence in duplex DNA, and then carry out exchange of the bound ssDNA strand with the like strand of the homologous duplex 3 . (nature.com)
  • Parafusin is a phosphoglucomutase (PGM) paralog that acts as a signaling scaffold protein in calcium mediated exocytosis across many eukaryotes. (biorxiv.org)
  • Freiburg: fibrillin-1 waste DNA as a second role of fluctuation-dissipation protein. (erik-mill.de)
  • This inhibits the formation of thymidylate and purines and arrests DNA, RNA, and protein synthesis.Common toxicities include mucositis and myelosuppression. (medscape.com)
  • Pemetrexed disrupts the folate-dependant metabolic processes important for cell replication, inhibits the enzymes involved in folate metabolism and DNA synthesis, and inhibits protein synthesis. (medscape.com)
Fluorescent4
  • To provide the appropriate specificity, strategies have been designed to ensure that the fluorescent signal is "off" with no miRNA signal is present and "on" when the target is present. (genalyte.com)
  • When present, the target miRNA displaced the quenching strand and turned "on" the fluorescent signal. (genalyte.com)
  • The DNA duplex binding agent used m the assay is typically an intercalating dye, for example SYBRGreen such as SYBRGreen I, SYBRGold, ethidium bromide and YOPRO-1, which are themselves fluorescent. (allindianpatents.com)
  • The fluorescent signals produced by the molecules used as donor and/or acceptor can be represented as peaks within the visible spectrum. (allindianpatents.com)
Genes2
  • However, the presence of a PbGR2 splice variant that lacks this insert, as well as the loss of the exon encoding these amino acids in the genes encoding for other teleost GR2 suggests the selection of two receptors with different DNA-binding domain structures in teleosts. (biomedcentral.com)
  • At 2 and 4 weeks after injury, muscles were examined histologically and apoptosis was measured using TUNEL assay and PCR array for a range of apoptotic genes. (biomedcentral.com)
LAMP1
  • In this study, LAMP assay that gives more consistent results for on-site diagnosis of fire blight than the previous developed LAMP assays was developed. (ppjonline.org)
Pathway1
  • Basic region/leucine zipper (bZIP) transcription factors play important roles in the abscisic acid (ABA) signaling pathway and abiotic stress response in plants. (usda.gov)
Synthesis3
  • They interfere with DNA synthesis by blocking the methylation of deoxyuridylic acid. (medscape.com)
  • Vinca alkaloids act on the M and S phases of mitosis, inhibiting microtubule formation and inhibiting DNA/RNA synthesis. (medscape.com)
  • Anthracycline antineoplastics inhibit DNA and RNA synthesis by steric obstruction. (medscape.com)
Receptors1
  • To elucidate the contribution of CRL3-ABA-mediated responses, we attempted to find CRL3 substrate receptors involved in ABA signaling. (usda.gov)
Restriction1
Double stranded1
  • The family Mimiviridae is comprised of double stranded DNA viruses up to 750 nm of diameter with genomes containing up to 1.2 Mb. (biomedcentral.com)
Probe5
  • Specimens containing the target DNA hybridize with the HR or LR HPV RNA probe cocktail. (cdc.gov)
  • In this method, a DNA duplex binding agent and a probe specific for said target sequence, is added to the sample. (allindianpatents.com)
  • This mixture is then subjected to an amplification reaction in which target nucleic acid is amplified, and conditions are induced (either during or after the amplification process in which the probe hybridises to the target sequence. (allindianpatents.com)
  • As the probe hybridises to the target sequence, DNA duplex binding agent such as an intercalating dye is trapped between the strands. (allindianpatents.com)
  • The signal from the reactive molecule on the probe is a strand specific signal, indicative of the presence of target within the sample. (allindianpatents.com)
RAPD1
Expression1
  • Mechanistically, unique VTT expression of the protease ADAMTS18 is necessary for VEGFA signaling sequestration through limiting fibronectin accumulation. (icr.ac.uk)
Duplex1
  • DNA duplex binding agents, which may be used in the pxocessr are any entity which adheres or associates itself with DNft in duplex form and which is capable of acting as an energy donor or acceptor. (allindianpatents.com)
Methylation1
  • 2-HG competitively inhibits enzymes that use αKG as a cofactor, such as TET family of enzymes and Jmj-C domain containing histone demethylases, leading to DNA hypermethylation, and aberrant methylation of a number of histone marks along with impaired differentiation leading to an expansion of stem/progenitor cells [ 10 , 27 , 40 ]. (biomedcentral.com)
Roles2
  • Here, we use a separation-of-function allele of RAD51 that retains DNA binding, but not D-loop activity, to reveal mechanistic aspects of RAD51's roles in the response to replication stress. (nature.com)
  • In this chapter, we reflect on our early understanding of the immunogenic properties of dsDNA and give a chronological account of the journey we have taken to discover the individual cellular DNA sensors which have played important roles in mediating DNA induced inflammation. (mewsie.org)
Antibodies1
  • The resultant RNA: DNA hybrids are captured onto the surface of a microplate well coated with antibodies specific for RNA: DNA hybrids. (cdc.gov)
Clinical2
  • Downstream next-generation sequencing (NGS) of the syphilis spirochete Treponema pallidum subspecies pallidum (T. pallidum) is hindered by low bacterial loads and the overwhelming presence of background metagenomic DNA in clinical specimens. (cdc.gov)
  • The emergence of these two newly recognized tickborne infections as threats to human health is probably due to increased clinical cognizance, but as in other emerging tickborne infections, it is likely that the rapid increase in identified cases signals a true emergence of disease associated with a changing vector-host ecology. (cdc.gov)
Incorporation1
  • After intracellular metabolism to its active nucleotide, it inhibits ribonucleotide reductase and competes with deoxycytidine triphosphate for incorporation into DNA. (medscape.com)
Donor1
  • The residual risk of HCV declined over the last decade due to improved screening reagents, implementation of the nucleic acid amplification test, and tight application of strict donor selection procedures. (biomedcentral.com)
Involves1
  • Replication fork regression involves branch migration in the direction opposite to replication to form a Holliday junction-containing chicken foot structure. (nature.com)
Host3
  • E. amylovora causes wilting and blight on most of the above-ground parts of host plants, including flowers, leaves, fruits, and branches. (ppjonline.org)
  • Microbial and host DNA are potent stimulators of innate immune responses and have been implicated in both host defense and autoimmune diseases. (mewsie.org)
  • To date more than 10 immunological sensors of DNA have been proposed, and we are now beginning to understand the functions and mechanism of action of these proposed DNA sensors in host defense and diseases. (mewsie.org)
Infection1
  • These assays are 97% specific but cannot distinguish acute from chronic infection. (medscape.com)
Sequencing2
  • To address this question, we performed the first epigenetic profiling of single cells in a cohort of 5 gliomas with IDH1 mutation using single nucleus Assay for Transposase-Accessible Chromatin with high-throughput sequencing (snATAC-seq). (biomedcentral.com)
  • To address this question, we interrogated the accessible chromatin at the individual cell level in gliomas with IDH mutation using single nucleus Assay for Transposase-Accessible Chromatin with high-throughput sequencing (snATAC-seq) on a subset of 5 patient samples. (biomedcentral.com)
EMERGING1
  • Finally, we extend the discussion beyond herpesviruses, and propose important questions that need to be addressed in the emerging field of innate immune activation by DNA. (mewsie.org)
Present1
  • ArGR, PbGR1 and PbGR2 all possess the unique 9 amino acid insert between the zinc-fingers of the DNA-binding domain that is present in one of the teleost GR lineages (GR1), but not the other (GR2). (biomedcentral.com)
Detect1
  • When you lost out to be with level, you DETECT to check second of the DISSERTATION that it is down to two signals: What lets the base of PerlMonth fowl? (ligaya-technologies.com)
Breast1
  • Estrogen and progesterone receptor (ER, PR) signaling control breast development and impinge on breast carcinogenesis. (icr.ac.uk)
Cell2
  • The assay allows for the isolation of high quality RNA suitable for accurate RNA-Seq of heterogeneous rare cell populations. (oncotarget.com)
  • Here we report that perivascular LGR5+ villus tip telocytes (VTTs) are necessary for maintenance of villus tip endothelial cell polarization and fenestration by sequestering VEGFA signaling. (icr.ac.uk)
Specific1
  • Excision repair cross-complementing 1 (ERCC1) is a structure-specific DNA repair endonuclease responsible for the 5-incision during DNA excision repair. (spandidos-publications.com)
Domain2
  • A 9 amino acid insert in the DNA-binding domain emerged in basal ray-finned fish GRs. (biomedcentral.com)
  • ADAMTS18+ villus tip telocytes maintain a polarized VEGFA signaling domain and fenestrations in nutrient-absorbing intestinal blood vessels. (icr.ac.uk)
Control1
  • I'm Commander Ibad Khan, and I'm representing the Clinician Outreach and Communication Activity, COCA, with the Emergency Risk Communication Branch at the Centers for Disease Control and Prevention. (cdc.gov)
Blood1
  • Therefore, we propose a model in which LGR5+ ADAMTS18+ telocytes are necessary to maintain a "just-right" level and location of VEGFA signaling in intestinal villus blood vasculature to ensure on one hand the presence of sufficient endothelial fenestrae, while avoiding excessive leakiness of the vessels and destabilization of villus tip epithelial structures. (icr.ac.uk)
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EpCAM based capture detects and recovers circulating tumor cells from all subtypes of breast cancer except claudin-low |... (oncotarget.com)
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Immunochemistry and Molecular Biology in Cytological Diagnosis | Basicmedical Key (basicmedicalkey.com)
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Cytomegalovirus (CMV) Retinitis Guidelines: Guidelines Summary (emedicine.medscape.com)
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Cytomegalovirus (CMV) Retinitis: Practice Essentials, Background, Pathophysiology (emedicine.medscape.com)
Cytomegalovirus (CMV) Retinitis: Practice Essentials, Background, Pathophysiology
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Hepatitis C Workup: Approach Considerations, Hepatitis C Antibody Test, Qualitative and Quantitative Assays for HCV RNA (medscape.com)
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HIV Testing Overview: Overview, HIV Virion and Genome, HIV Testing Recommendations (medscape.com)