Brachyspira hyodysenteriae: A species of anaerobic, spiral bacteria that was formerly classified as Serpulina hyodysenteriae and Treponema hyodysenteriae (and for a short while, Serpula hyodysenteriae). This organism is the agent of swine dysentery.Brachyspira: A genus of spiral bacteria of the family Brachyspiraceae.Spirochaetales Infections: Infections with bacteria of the order SPIROCHAETALES.Spirochaetales: An order of slender, flexuous, helically coiled bacteria, with one or more complete turns in the helix.Dysentery: Acute inflammation of the intestine associated with infectious DIARRHEA of various etiologies, generally acquired by eating contaminated food containing TOXINS, BIOLOGICAL derived from BACTERIA or other microorganisms. Dysentery is characterized initially by watery FECES then by bloody mucoid stools. It is often associated with ABDOMINAL PAIN; FEVER; and DEHYDRATION.Swine Diseases: Diseases of domestic swine and of the wild boar of the genus Sus.Desulfovibrionaceae Infections: Infections with bacteria of the family Desulfovibrionaceae.Treponemal Infections: Infections with bacteria of the genus TREPONEMA.Gram-Negative Bacterial Infections: Infections caused by bacteria that show up as pink (negative) when treated by the gram-staining method.Lawsonia Bacteria: A genus of gram-negative, obligate intracellular bacteria causing a proliferative enteritis in animals, especially pigs, deer, horses, and rabbits.Treponema: A genus of microorganisms of the order SPIROCHAETALES, many of which are pathogenic and parasitic for man and animals.Swine: Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).Spirochaetaceae: A family of spiral bacteria of the order SPIROCHAETALES.Carbadox: An antibacterial agent that has been used in veterinary practice for treating swine dysentery and enteritis and for promoting growth. However, its use has been prohibited in the UK following reports of carcinogenicity and mutagenicity. (From Martindale, The Extra Pharmacopoeia, 30th ed, p125)Spirochaeta: A genus of flexible, spiral rods found in hydrogen sulfide-containing mud, sewage, and polluted water. None of the species properly referred to in this genus are pathogenic.Intestinal Diseases: Pathological processes in any segment of the INTESTINE from DUODENUM to RECTUM.Cecum: The blind sac or outpouching area of the LARGE INTESTINE that is below the entrance of the SMALL INTESTINE. It has a worm-like extension, the vermiform APPENDIX.Methane: The simplest saturated hydrocarbon. It is a colorless, flammable gas, slightly soluble in water. It is one of the chief constituents of natural gas and is formed in the decomposition of organic matter. (Grant & Hackh's Chemical Dictionary, 5th ed)Mesna: A sulfhydryl compound used to prevent urothelial toxicity by inactivating metabolites from ANTINEOPLASTIC AGENTS, such as IFOSFAMIDE or CYCLOPHOSPHAMIDE.Diagnostic Tests, Routine: Diagnostic procedures, such as laboratory tests and x-rays, routinely performed on all individuals or specified categories of individuals in a specified situation, e.g., patients being admitted to the hospital. These include routine tests administered to neonates.Animal Testing Alternatives: Procedures, such as TISSUE CULTURE TECHNIQUES; mathematical models; etc., when used or advocated for use in place of the use of animals in research or diagnostic laboratories.Canada: The largest country in North America, comprising 10 provinces and three territories. Its capital is Ottawa.Animal Welfare: The protection of animals in laboratories or other specific environments by promoting their health through better nutrition, housing, and care.Animal Experimentation: The use of animals as investigational subjects.Animals, LaboratoryAnimal Use Alternatives: Alternatives to the use of animals in research, testing, and education. The alternatives may include reduction in the number of animals used, replacement of animals with a non-animal model or with animals of a species lower phylogenetically, or refinement of methods to minimize pain and distress of animals used.Animal Care Committees: Institutional committees established to protect the welfare of animals used in research and education. The 1971 NIH Guide for the Care and Use of Laboratory Animals introduced the policy that institutions using warm-blooded animals in projects supported by NIH grants either be accredited by a recognized professional laboratory animal accrediting body or establish its own committee to evaluate animal care; the Public Health Service adopted a policy in 1979 requiring such committees; and the 1985 amendments to the Animal Welfare Act mandate review and approval of federally funded research with animals by a formally designated Institutional Animal Care and Use Committee (IACUC).GermanyMaleatesReflex, Vestibulo-Ocular: A reflex wherein impulses are conveyed from the cupulas of the SEMICIRCULAR CANALS and from the OTOLITHIC MEMBRANE of the SACCULE AND UTRICLE via the VESTIBULAR NUCLEI of the BRAIN STEM and the median longitudinal fasciculus to the OCULOMOTOR NERVE nuclei. It functions to maintain a stable retinal image during head rotation by generating appropriate compensatory EYE MOVEMENTS.Cerebral Hemorrhage: Bleeding into one or both CEREBRAL HEMISPHERES including the BASAL GANGLIA and the CEREBRAL CORTEX. It is often associated with HYPERTENSION and CRANIOCEREBRAL TRAUMA.STAT3 Transcription Factor: A signal transducer and activator of transcription that mediates cellular responses to INTERLEUKIN-6 family members. STAT3 is constitutively activated in a variety of TUMORS and is a major downstream transducer for the CYTOKINE RECEPTOR GP130.Fibrinolytic Agents: Fibrinolysin or agents that convert plasminogen to FIBRINOLYSIN.Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.Iodide Peroxidase: A hemeprotein that catalyzes the oxidation of the iodide radical to iodine with the subsequent iodination of many organic compounds, particularly proteins. EC 1.11.1.8.Thyronines: A group of metabolites derived from THYROXINE and TRIIODOTHYRONINE via the peripheral enzymatic removal of iodines from the thyroxine nucleus. Thyronine is the thyroxine nucleus devoid of its four iodine atoms.Patents as Topic: Exclusive legal rights or privileges applied to inventions, plants, etc.EstersThyroxine: The major hormone derived from the thyroid gland. Thyroxine is synthesized via the iodination of tyrosines (MONOIODOTYROSINE) and the coupling of iodotyrosines (DIIODOTYROSINE) in the THYROGLOBULIN. Thyroxine is released from thyroglobulin by proteolysis and secreted into the blood. Thyroxine is peripherally deiodinated to form TRIIODOTHYRONINE which exerts a broad spectrum of stimulatory effects on cell metabolism.Triiodothyronine, Reverse: A metabolite of THYROXINE, formed by the peripheral enzymatic monodeiodination of T4 at the 5 position of the inner ring of the iodothyronine nucleus.Drug Carriers: Forms to which substances are incorporated to improve the delivery and the effectiveness of drugs. Drug carriers are used in drug-delivery systems such as the controlled-release technology to prolong in vivo drug actions, decrease drug metabolism, and reduce drug toxicity. Carriers are also used in designs to increase the effectiveness of drug delivery to the target sites of pharmacological actions. Liposomes, albumin microspheres, soluble synthetic polymers, DNA complexes, protein-drug conjugates, and carrier erythrocytes among others have been employed as biodegradable drug carriers.Veterinary Medicine: The medical science concerned with the prevention, diagnosis, and treatment of diseases in animals.Fascioliasis: Liver disease caused by infections with parasitic flukes of the genus FASCIOLA, such as FASCIOLA HEPATICA.Education, Veterinary: Use for general articles concerning veterinary medical education.Intestine, Large: A segment of the LOWER GASTROINTESTINAL TRACT that includes the CECUM; the COLON; and the RECTUM.Diarrhea: An increased liquidity or decreased consistency of FECES, such as running stool. Fecal consistency is related to the ratio of water-holding capacity of insoluble solids to total water, rather than the amount of water present. Diarrhea is not hyperdefecation or increased fecal weight.Ileitis: Inflammation of any segment of the ILEUM and the ILEOCECAL VALVE.Virginiamycin: A cyclic polypeptide antibiotic complex from Streptomyces virginiae, S. loidensis, S. mitakaensis, S. pristina-spiralis, S. ostreogriseus, and others. It consists of 2 major components, VIRGINIAMYCIN FACTOR M1 and virginiamycin Factor S1. It is used to treat infections with gram-positive organisms and as a growth promoter in cattle, swine, and poultry.Immunization: Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).Food Additives: Substances which are of little or no nutritive value, but are used in the processing or storage of foods or animal feed, especially in the developed countries; includes ANTIOXIDANTS; FOOD PRESERVATIVES; FOOD COLORING AGENTS; FLAVORING AGENTS; ANTI-INFECTIVE AGENTS (both plain and LOCAL); VEHICLES; EXCIPIENTS and other similarly used substances. Many of the same substances are PHARMACEUTIC AIDS when added to pharmaceuticals rather than to foods.Streptogramin Group B: A structural subclass of streptogramins. They are cyclic hexadepsipeptides with approximate molecular weight of 800 that contain a characteristic 3-hydroxy picolinoyl exocyclic component.Plectonema: A form-genus of CYANOBACTERIA in the order Oscillatoriales. They are non-heterocystous but may carry out NITROGEN FIXATION under microaerobic conditions. Some smaller members have undergone transfer to the form-genus Leptolyngbya.Streptogramins: A class of natural cyclic peptide antibiotics produced by certain subspecies of STREPTOMYCES. They include two structurally unrelated components, STREPTOGRAMIN GROUP A and STREPTOGRAMIN GROUP B, which generally act synergistically to inhibit bacterial growth.

Analysis of Serpulina hyodysenteriae strain variation and its molecular epidemiology using pulsed-field gel electrophoresis. (1/81)

Pulsed-field gel electrophoresis (PFGE) was applied as a molecular typing tool for the spirochaete Serpulina hyodysenteriae, the agent of swine dysentery. Analysis of a collection of 40 mainly Australian isolates, previously characterized by other methods, divided these into 23 PFGE types. This confirmed that there are many strains of the spirochaete in Australia. PFGE was more discriminatory for strain typing than both multilocus enzyme electrophoresis and serotyping. It had similar discriminatory power to restriction endonuclease analysis, but the results of PFGE were easier to interpret. When applied to 29 isolates collected from 4 farms over periods of up to 8 years, 2 PFGE patterns were found on 3 farms, and a single pattern on the other. In each case a new strain had apparently emerged as a variant of an original parent strain. PFGE was found to be a powerful technique for investigating the molecular epidemiology of swine dysentery outbreaks.  (+info)

A comparison of the morphologic effects of Serpulina hyodysenteriae or its beta-hemolysin on the murine cecal mucosa. (2/81)

Studies were carried out to compare the early morphologic changes in the cecal mucosa of mice either infected with Serpulina hyodysenteriae or exposed to the beta-hemolysin of S. hyodysenteriae. Sixty-five 12-24-week-old C3H/HeOuJ mice were infected with S. hyodysenteriae by gastric intubation. Two mice were necropsied every hour for 30 hours following infection. S. hyodysenteriae was isolated from the cecal contents of each mouse at all time points. Macroscopic lesions were first apparent at 14 hours postinfection (PI), and light microscopic lesions were first apparent at 10 hours PI, earlier than has been previously reported. Ultrastructural changes, first evident at 6 hours PI, included disarray and loss of microvilli and terminal web, with dilatation of intercellular spaces. Luminal bacteria were translocated through epithelial cells to the lamina propria, where capillaries exhibited changes indicative of increased permeability. In another experiment, solutions containing between 2,500 and 25,000 hemolytic units of purified S. hyodysenteriae hemolysin were placed within the lumen of surgically closed murine ceca (n = 10); ceca were collected for examination 3 hours following treatment. Ultrastructural changes consisted of loss of microvilli and terminal web and marked vacuolation and exfoliation of epithelial cells. Significant numbers of necrotic and apoptotic epithelial cells were present, and epithelial cells internalized moderate numbers of bacteria. The hemolysin of S. hyodysenteriae induces some of the same early ultrastructural changes in the cecal epithelium of mice as occur following infection with S. hyodysenteriae. Based on the observed bacterial translocation, luminal bacteria also appear to play a unique role in lesion development in this model.  (+info)

Isolation, oxygen sensitivity, and virulence of NADH oxidase mutants of the anaerobic spirochete Brachyspira (Serpulina) hyodysenteriae, etiologic agent of swine dysentery. (3/81)

Brachyspira (Serpulina) hyodysenteriae, the etiologic agent of swine dysentery, uses the enzyme NADH oxidase to consume oxygen. To investigate possible roles for NADH oxidase in the growth and virulence of this anaerobic spirochete, mutant strains deficient in oxidase activity were isolated and characterized. The cloned NADH oxidase gene (nox; GenBank accession no. U19610) on plasmid pER218 was inactivated by replacing 321 bp of coding sequence with either a gene for chloramphenicol resistance (cat) or a gene for kanamycin resistance (kan). The resulting plasmids, respectively, pCmDeltaNOX and pKmDeltaNOX, were used to transform wild-type B. hyodysenteriae B204 cells and generate the antibiotic-resistant strains Nox-Cm and Nox-Km. PCR and Southern hybridization analyses indicated that the chromosomal wild-type nox genes in these strains had been replaced, through allelic exchange, by the inactivated nox gene containing cat or kan. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western immunoblot analysis revealed that both nox mutant cell lysates were missing the 48-kDa Nox protein. Soluble NADH oxidase activity levels in cell lysates of Nox-Cm and Nox-Km were reduced 92 to 96% compared to the activity level in parent strain B204. In an aerotolerance test, cells of both nox mutants were at least 100-fold more sensitive to oxygen exposure than were cells of the wild-type parent strain B204. In swine experimental infections, both nox mutants were less virulent than strain B204 in that fewer animals were colonized by the mutant cells and infected animals displayed mild, transient signs of disease, with no deaths. These results provide evidence that NADH oxidase serves to protect B. hyodysenteriae cells against oxygen toxicity and that the enzyme, in that role, contributes to the pathogenic ability of the spirochete.  (+info)

Characterization of a periplasmic ATP-binding cassette iron import system of Brachyspira (Serpulina) hyodysenteriae. (4/81)

The nucleotide sequence of the pathogenic spirochete Brachyspira hyodysenteriae bit (for "Brachyspira iron transport") genomic region has been determined. The bit region is likely to encode an iron ATP-binding cassette transport system with some homology to those encountered in gram-negative bacteria. Six open reading frames oriented in the same direction and physically linked have been identified. This system possesses a protein containing ATP-binding motifs (BitD), two hydrophobic cytoplasmic membrane permeases (BitE and BitF), and at least three lipoproteins (BitA, BitB, and BitC) with homology to iron periplasmic binding proteins. These periplasmic binding proteins exhibit lipoprotein features. They are labeled by [(3)H]palmitate when tested in recombinant Escherichia coli, and their signal peptides are typical for substrates of the type II secretory peptidase. The FURTA system and Congo red assay indicate that BitB and BitC are involved in iron binding. The Bit system is detected only in B. hyodysenteriae and is absent from B. innocens and B. pilosicoli.  (+info)

Changes in bacterial community structure in the colon of pigs fed different experimental diets and after infection with Brachyspira hyodysenteriae. (5/81)

Bacterial communities in the large intestines of pigs were compared using terminal restriction fragment length polymorphism (T-RFLP) analysis targeting the 16S ribosomal DNA. The pigs were fed different experimental diets based on either modified standard feed or cooked rice supplemented with dietary fibers. After feeding of the animals with the experimental diets for 2 weeks, differences in the bacterial community structure in the spiral colon were detected in the form of different profiles of terminal restriction fragments (T-RFs). Some of the T-RFs were universally distributed, i.e., they were found in all samples, while others varied in distribution and were related to specific diets. The reproducibility of the T-RFLP profiles between individual animals within the diet groups was high. In the control group, the profiles remained unchanged throughout the experiment and were similar between two independent but identical experiments. When the animals were experimentally infected with Brachyspira hyodysenteriae, causing swine dysentery, many of the T-RFs fluctuated, suggesting a destabilization of the microbial community.  (+info)

The spirochete FlaA periplasmic flagellar sheath protein impacts flagellar helicity. (6/81)

Spirochete periplasmic flagella (PFs), including those from Brachyspira (Serpulina), Spirochaeta, Treponema, and Leptospira spp., have a unique structure. In most spirochete species, the periplasmic flagellar filaments consist of a core of at least three proteins (FlaB1, FlaB2, and FlaB3) and a sheath protein (FlaA). Each of these proteins is encoded by a separate gene. Using Brachyspira hyodysenteriae as a model system for analyzing PF function by allelic exchange mutagenesis, we analyzed purified PFs from previously constructed flaA::cat, flaA::kan, and flaB1::kan mutants and newly constructed flaB2::cat and flaB3::cat mutants. We investigated whether any of these mutants had a loss of motility and altered PF structure. As formerly found with flaA::cat, flaA::kan, and flaB1::kan mutants, flaB2::cat and flaB3::cat mutants were still motile, but all were less motile than the wild-type strain, using a swarm-plate assay. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analysis indicated that each mutation resulted in the specific loss of the cognate gene product in the assembled purified PFs. Consistent with these results, Northern blot analysis indicated that each flagellar filament gene was monocistronic. In contrast to previous results that analyzed PFs attached to disrupted cells, purified PFs from a flaA::cat mutant were significantly thinner (19.6 nm) than those of the wild-type strain and flaB1::kan, flaB2::cat, and flaB3::cat mutants (24 to 25 nm). These results provide supportive genetic evidence that FlaA forms a sheath around the FlaB core. Using high-magnification dark-field microscopy, we also found that flaA::cat and flaA::kan mutants produced PFs with a smaller helix pitch and helix diameter compared to the wild-type strain and flaB mutants. These results indicate that the interaction of FlaA with the FlaB core impacts periplasmic flagellar helical morphology.  (+info)

Antigen-specific proliferation of porcine CD8alphaalpha cells to an extracellular bacterial pathogen. (7/81)

A vaccine inducing protective immunity to a spirochaete-induced colitis of pigs predominantly stimulates expansion of CD8+ cells in vivo and in antigen-stimulated lymphocyte cultures. CD8+ cells, however, are rarely considered necessary for protection against extracellular bacterial pathogens. In the present study, pigs recovering from colitis resulting from experimental infection with Brachyspira (Serpulina) hyodysenteriae had increased percentages of peripheral blood CD4- CD8+ (alphaalpha-expressing) cells compared with non-infected pigs. CD8alphaalpha+ cells proliferated in antigen-stimulated cultures of peripheral blood mononuclear cells from B. hyodysenteriae-vaccinated pigs. Proliferating CD8alphaalpha+ cells consisted of CD4-, CD4+ and gammadelta T-cell receptor-positive cells. CD4- CD8alphabeta+ cells from vaccinated or infected pigs did not proliferate upon in vitro antigen stimulation. Of the CD8alphaalpha cells that had proliferated, flow cytometric analysis indicated that the majority of the CD4+ CD8+ cells were large (i.e. lymphoblasts) whereas the CD4- CD8+ cells were predominantly small. Addition of monoclonal antibodies (mAb) specific for either porcine major histocompatibility complex (MHC) class I or class II antigens diminished B. hyodysenteriae-specific proliferative responses whereas addition of mAb to porcine MHC II, but not porcine MHC I, reduced the CD8alphaalpha response. In vitro depletion of CD4+ cells by flow cytometric cell sorting diminished, but did not completely abrogate, the proliferative response of cells from vaccinated pigs to B. hyodysenteriae antigen stimulation. These results suggest that CD8alphaalpha cells are involved in recovery and possibly protection from a spirochaete-induced colitis of pigs; yet, this response appears to be partially dependent upon CD4+ cells.  (+info)

Cloning of a beta-hemolysin gene of Brachyspira (Serpulina) hyodysenteriae and its expression in Escherichia coli. (8/81)

Brachyspira (Serpulina) hyodysenteriae induces a mucohemorrhagic diarrheal disease in pigs. The production of a beta-hemolysin has been considered a major virulence attribute of this organism. Previous reports have failed to correlate a specific cloned gene sequence with a purified beta-hemolytic protein sequence. Thus, questions still remain concerning the structural gene sequence of the hemolysin. To answer this question unequivocally, the beta-hemolytic toxin was purified from extracts of log-phase spirochetes, and the N-terminal amino acid sequence was determined (K-D-V-V-A-N-Q-L-N-I-S-D-K) and compared with the translated sequences of previously cloned genes, tlyA to tlyC. The lack of homology between tlyA to tlyC translated sequences and the purified beta-hemolytic toxin sequence resulted in the study that is reported here. A degenerate probe was designed based on the N-terminal amino acid sequence of the purified beta-hemolysin and used to screen a B. hyodysenteriae genomic library. Three overlapping clones were identified, and one was sequenced to reveal an open reading frame coding for a putative 8.93-kDa polypeptide containing the N-terminal sequence of the purified beta-hemolysin. To distinguish this gene from the tlyA to tlyC genes, it has been designated hlyA. A hemolysis-negative Escherichia coli strains containing hlyA was beta-hemolytic on blood agar media. Also, the hemolytic activity of the recombinant protein had identical protease and lipase sensitivities and electrophoretic mobility to those of native B. hyodysenteriae beta-hemolysin. Based on sequence analysis, the translated protein had a pI of 4.3, an alpha-helical structure, and a phosphopantetheine binding motif. Hybridization analysis of genomic DNA indicated that the hlyA gene was present in B. hyodysenteriae and B. intermedia but was not detected in B. innocens, B. pilosicoli, or B. murdochii under high-stringency conditions. The location of hlyA on the chromosomal map was distinct from the locations of tlyA, tlyB, and tlyC.  (+info)

1992) Virulent Serpulina hyodysenteriae from a pig in a herd free of clinical swine dysentery. Veterinary Record, 131 (14). pp. 318-319. ...
For swine dysentery, which is caused by Brachyspira hyodysenteriae infection and is an economically important disease in intensive pig production systems worldwide, a perfect or error-free diagnostic test (gold standard) is not available. In the absence of a gold standard, Bayesian latent class modelling is a well-established methodology for robust diagnostic test evaluation. In contrast to risk factor studies in food animals, where adjustment for within group correlations is both usual and required for good statistical practice, diagnostic test evaluation studies rarely take such clustering aspects into account, which can result in misleading results. The aim of the present study was to estimate test accuracies of a PCR originally designed for use as a confirmatory test, displaying a high diagnostic specificity, and cultural examination for B. hyodysenteriae. This estimation was conducted based on results of 239 samples from 103 herds originating from routine diagnostic sampling. Using ...
A fluorescent-labelledin situ hybridization method targeting rRNA was devised to facilitate specific identification and diagnosis of diarrhoea and colitis in pigs caused by the genus Serpulina, as well as to distinguish the species Serpulina hyodysenteriae and Serpulina pilosicoli in formalin-fixed colon tissue sections. A genus-specific oligonucleotide probe SER1410 targeting the five species of porcine Serpulina was thus designed. Furthermore, species specific oligonucleotide probes (Hyo1210, Pilosi209 and Pilosi1405) were also designed to detect, identify and differentiate S. hyodysenteriae and S. pilosicoli. These probes clearly demonstrated and possessed the desired specificity, when evaluated by whole cell hybridization on five reference strains and 20 isolates covering the five species of porcine Serpulina. Furthermore, the oligonucleotide probes were specific when used both, for the detection of Serpulina isolates at genus level as well as for specific detection of S. hyodysenteriae and ...
Swine dysentery (SD) is an economically important diarrheal disease in pigs caused by different strongly hemolytic Brachyspira (B.) species, such as B. hyodysenteriae, B. suanatina and B. hampsonii. Possible associations of epidemiologic data, such as multilocus sequence types (STs) to virulence gene profiles and antimicrobial susceptibility are rather scarce, particularly for B. hyodysenteriae isolates from Germany. In this study, B. hyodysenteriae (n = 116) isolated from diarrheic pigs between 1990 and 2016 in Germany were investigated for their STs, susceptibility to the major drugs used for treatment of SD (tiamulin and valnemulin) and genes that were previously linked with virulence and encode for hemolysins (tlyA, tlyB, tlyC, hlyA, BHWA1_RS02885, BHWA1_RS09085, BHWA1_RS04705, and BHWA1_RS02195), outer membrane proteins (OMPs) (bhlp16, bhlp17 ...
BioAssay record AID 205190 submitted by ChEMBL: In vitro antibacterial activity against Serpulina (Treponema) hyodysenteriae was tested and is expressed as minimum inhibitory concentration: Value ranges from (50-100 ug/mL).
Valnemulin hydrochloride is a pleuromutilin antibiotic which inhibits protein synthesis in bacteria by binding the peptidyl transferase enzyme in the 50s ribosomal subunit. - Mechanism of Action & Protocol.
An experiment was conducted to study the effect of diets with contrasting fermentability in the large intestine on experimental infections with Brachyspira hyodysenteriae, the causative agent of swine dysentery, and the whip worm, Trichuris suis, in pigs. Two diets with organically grown ingredients were composed. Both diets were based on triticale and barley and supplemented with either rape seed cake (Diet 1) or dried chicory root and sweet lupins (Diet 2). The study had a three-factorial design, with eight groups of pigs receiving Diet I or Diet 2, +/- B. hyodysenteriae, and +/- T suis. Pigs fed Diet 2 and challenged with B. hyodysenteriae did not develop swine dysentery and B. hyodysenteriae was not demonstrated in any of the pigs during the study. In contrast, 94% of the B. hyodysenteriae challenged pigs fed Diet I showed clinical symptoms of swine dysentery and all the pigs were shedding B. hyodysenteriae in faeces at some points in time during the experiment. The number of T suis was ...
Methane is metabolized principally by methanotrophs and methanogens in the global carbon cycle. Methanotrophs consume methane as the only source of carbon, while methanogens produce methane as a metabolic byproduct. Methylotrophs, which are microorganisms that can obtain energy for growth by oxidizing one-carbon compounds, such as methanol and methane, are situated between methanotrophs and methanogens. Methanogens can obtain energy for growth by converting a limited number of substrates to methane under anaerobic conditions. Three types of methanogenic pathways are known: CO2 to methane [MD:M00567], methanol to methane [MD:M00356], and acetate to methane [MD:M00357]. Methanogens use 2-mercaptoethanesulfonate (CoM; coenzyme M) as the terminal methyl carrier in methanogenesis and have four enzymes for CoM biosynthesis [MD:M00358]. Coenzyme B-Coenzyme M heterodisulfide reductase (Hdr), requiring for the final reaction steps of methanogenic pathway, is divided into two types: cytoplasmic HdrABC in ...
Multilocus enzyme electrophoresis was used to determine genetic relationships amongst 32 intestinal spirochaetes (Serpulina spp.) isolated from rats (17), rheas (7), chickens, (4), ducks (2), a swan (1) and a flamingo (1). The strains were divided into 20 electrophoretic types (ETs), with a mean genetic diversity per locus of 0.62. The results were compared with those previously published for porcine intestinal spirochaetes. One strain from a healthy rat, and three rhea strains which were recovered from cases of necrotizing typhlitis, were grouped in the same ETs as certain porcine strains of Serpulina hyodysenteriae. The rhea strains could be differentiated from these by pulsed-field gel electrophoresis. Fifteen of the rat strains were genetically and phenotypically closely related. In contrast the avian strains were genetically more heterogeneous, with pathogenic isolates located in three different genetic groups.. ...
Forty-eight feeder pigs were used in an experiment to determine Virginiamycins effectiveness in preventing swine dysentery. Thirteen of 24 controls and one of 24 that received the antibiotic at either 25 or 50 grams per ton of feed died of swine dysentery or complications. Feed conversion ratio was markedly improved by the antibiotic. Virginiamycin was judged effective in preventing clinical cases of swine dysentery, but it has not been cleared for use in swine. Swine dysentery, also known as bloody dysentery, vibrionic dysentery, bloody scours, or black scours, is a infectious, enteric disease of swine. It is serious in many areas of the Midwest and is assumed to be present wherever swine are raised in the United States. For years the etiology of the condition was thought to be Vibrio coli; however, recent work has indicated Treponema hyodysenterrae-instead ...
AHDB Pork and the National Pig Association have issued a timely reminder about the importance of signing up to the Significant Disease Charter, after the recent re-emergence Swine Dysentery in Yorkshire.. Two new cases of Swine Dysentery have been detected on Yorkshire farms in August.. Outbreaks were identified on a rearing unit in North Yorkshire on August 5 and on a finishing unit near Driffield on August 22.. The cases were identified by clinical signs and subsequently confirmed by laboratory tests.. The previous two cases identified in the UK were also found in Yorkshire, in April 2016 and November 2015. No further details are being given of the most recently affected farms, except to members of the Significant Disease Charter, which enables farmers to share information on serious pig diseases.. The charter is run by AHDB Pork on behalf of the Pig Health and Welfare Council and is an extension of the swine dysentery charter.. AHDB Pork veterinary manager Martin Smith said: The aim of the ...
Losses associated with gastric ulceration might warrant an ongoing monitoring program.The first step of a monitoring program is to ensure that the herdsmen are familiar with the clinical signs of gastric ulceration. Blood loss into the gastrointestinal tract, the main clinical event in this condition, may cause anemia and melena. Anemia may be severe, resulting in a very pale, weak pig with rapid breathing. The feces are generally scant, black, and tarry. A number of infectious agents (eg, Lawsonia intracellularis, Salmonella serovars, and Brachyspira hyodysenteriae) may induce lesions resulting in blood loss into the intestines, but these conditions are associated with diarrhea, which is not the case with gastric ulcers. Pigs with extensive and severe erosive lesions of the pars oesophagea may continue to appear healthy if blood loss is minimal. Damaged tissue heals rapidly. If the opening of the oesophagus into the stomach is restricted because of scar tissue formation, pigs may be observed to ...
The results of the present study show that experimental swine dysentery induces detectable levels of some key cytokines in the blood and that they vary regarding to the stage of the disease in which they first appear. The experimental model was successful and the hall-marks for this disease, e.g. muco-haemorrhagic diarrhoea, the shedding of B. hyodysenteriae in the faeces and the results from the necropsies confirm that the animals were suffering from swine dysentery. In addition, the high bio security at the experimental facility and the absence of clinical signs of disease during the long acclimatisation period further underscores that no overt co-infection was present during the experimental period.. IL-1β is commonly referred to as an endogenous pyrogen and is generally associated with pyrexia. However, swine dysentery does not in general appear to induce fever and in the present study the only animal with an elevated body temperature was the one with the most severe signs of dysentery. ...
Farmers have been warned to be vigilant and maintain high biosecurity standards after another case of Swine Dysentery was detected on a farm in Yorkshire.. The case was identified by clinical signs and subsequently confirmed by laboratory tests on Monday, August 22.. No details are being given of the location of the affected farm, except to members of the Significant Disease Charter. It is the second case to be reported in Yorkshire in a few weeks. The disease was identified on a rearing unit in North Yorkshire on August 5. The previous two cases identified in the UK were also in Yorkshire, in April 2016 and November 2015.. AHDB Pork is advising producers to increase vigilance for the development of clinical signs of disease within their herd.. It is important that a heightened level of biosecurity and monitoring for clinical signs are observed over the next few weeks, a spokesperson added.. BIOSECURITY GUIDANCE. ...
Swine dysentery is still a problem in some herds and in some countries, but it can be treated, controlled and eliminated. Improved methods of diagnosis have allowed increasingly sophisticated certification for herds free from infection. Plus, strict biosecurity can be supplemented in many countries by supplies of dysentery-free breeding stock to ensure that production is not interrupted by the disease.
Learn about the veterinary topic of Swine Dysentery. Find specific details on this topic and related topics from the Merck Vet Manual.
Acetohalobium arabaticum strain DSM 5501 Alkaliphilus metalliredigens strain QYMF Alkaliphilus oremlandii strain OhILAs Anaerococcus prevotii strain ACS-065-V-Col13 Anaerococcus vaginalis strain ATCC 51170 Anaerofustis stercorihominis strain DSM 17244 Anaerostipes caccae strain DSM 14662 Anaerostipes sp. strain 3_2_56FAA Anaerotruncus colihominis strain DSM 17241 Bacteroides capillosus strain ATCC 29799 Bacteroides pectinophilus strain ATCC 43243 Brachyspira hyodysenteriae strain ATCC 49526; WA1 Brachyspira intermedia strain PWS/A Brachyspira pilosicoli strain 95/1000 Candidatus Arthromitus sp. SFB-mouse-Japan Carnobacterium sp. strain 17-4 Clostridium acetobutylicum strain ATCC 824 Clostridium asparagiforme strain DSM 15981 Clostridium bartlettii strain DSM 16795 Clostridium bolteae strain ATCC BAA-613 Clostridium botulinum A2 strain Kyoto Clostridium butyricum strain 5521 Clostridium cellulovorans strain 743B Clostridium cf. saccharolyticum strain K10 Clostridium citroniae strain WAL-17108 ...
Acetohalobium arabaticum strain DSM 5501 Alkaliphilus metalliredigens strain QYMF Alkaliphilus oremlandii strain OhILAs Anaerococcus prevotii strain ACS-065-V-Col13 Anaerococcus vaginalis strain ATCC 51170 Anaerofustis stercorihominis strain DSM 17244 Anaerostipes caccae strain DSM 14662 Anaerostipes sp. strain 3_2_56FAA Anaerotruncus colihominis strain DSM 17241 Bacteroides capillosus strain ATCC 29799 Bacteroides pectinophilus strain ATCC 43243 Brachyspira hyodysenteriae strain ATCC 49526; WA1 Brachyspira intermedia strain PWS/A Brachyspira pilosicoli strain 95/1000 Candidatus Arthromitus sp. SFB-mouse-Japan Carnobacterium sp. strain 17-4 Clostridium acetobutylicum strain ATCC 824 Clostridium asparagiforme strain DSM 15981 Clostridium bartlettii strain DSM 16795 Clostridium bolteae strain ATCC BAA-613 Clostridium botulinum A2 strain Kyoto Clostridium butyricum strain 5521 Clostridium cellulovorans strain 743B Clostridium cf. saccharolyticum strain K10 Clostridium citroniae strain WAL-17108 ...
Acetohalobium arabaticum strain DSM 5501 Alkaliphilus metalliredigens strain QYMF Alkaliphilus oremlandii strain OhILAs Anaerococcus prevotii strain ACS-065-V-Col13 Anaerococcus vaginalis strain ATCC 51170 Anaerofustis stercorihominis strain DSM 17244 Anaerostipes caccae strain DSM 14662 Anaerostipes sp. strain 3_2_56FAA Anaerotruncus colihominis strain DSM 17241 Bacteroides capillosus strain ATCC 29799 Bacteroides pectinophilus strain ATCC 43243 Brachyspira hyodysenteriae strain ATCC 49526; WA1 Brachyspira intermedia strain PWS/A Brachyspira pilosicoli strain 95/1000 Candidatus Arthromitus sp. SFB-mouse-Japan Carnobacterium sp. strain 17-4 Clostridium acetobutylicum strain ATCC 824 Clostridium asparagiforme strain DSM 15981 Clostridium bartlettii strain DSM 16795 Clostridium bolteae strain ATCC BAA-613 Clostridium botulinum A2 strain Kyoto Clostridium butyricum strain 5521 Clostridium cellulovorans strain 743B Clostridium cf. saccharolyticum strain K10 Clostridium citroniae strain WAL-17108 ...
Acetohalobium arabaticum strain DSM 5501 Alkaliphilus metalliredigens strain QYMF Alkaliphilus oremlandii strain OhILAs Anaerococcus prevotii strain ACS-065-V-Col13 Anaerococcus vaginalis strain ATCC 51170 Anaerofustis stercorihominis strain DSM 17244 Anaerostipes caccae strain DSM 14662 Anaerostipes sp. strain 3_2_56FAA Anaerotruncus colihominis strain DSM 17241 Bacteroides capillosus strain ATCC 29799 Bacteroides pectinophilus strain ATCC 43243 Brachyspira hyodysenteriae strain ATCC 49526; WA1 Brachyspira intermedia strain PWS/A Brachyspira pilosicoli strain 95/1000 Candidatus Arthromitus sp. SFB-mouse-Japan Carnobacterium sp. strain 17-4 Clostridium acetobutylicum strain ATCC 824 Clostridium asparagiforme strain DSM 15981 Clostridium bartlettii strain DSM 16795 Clostridium bolteae strain ATCC BAA-613 Clostridium botulinum A2 strain Kyoto Clostridium butyricum strain 5521 Clostridium cellulovorans strain 743B Clostridium cf. saccharolyticum strain K10 Clostridium citroniae strain WAL-17108 ...
Acetohalobium arabaticum strain DSM 5501 Alkaliphilus metalliredigens strain QYMF Alkaliphilus oremlandii strain OhILAs Anaerococcus prevotii strain ACS-065-V-Col13 Anaerococcus vaginalis strain ATCC 51170 Anaerofustis stercorihominis strain DSM 17244 Anaerostipes caccae strain DSM 14662 Anaerostipes sp. strain 3_2_56FAA Anaerotruncus colihominis strain DSM 17241 Bacteroides capillosus strain ATCC 29799 Bacteroides pectinophilus strain ATCC 43243 Brachyspira hyodysenteriae strain ATCC 49526; WA1 Brachyspira intermedia strain PWS/A Brachyspira pilosicoli strain 95/1000 Candidatus Arthromitus sp. SFB-mouse-Japan Carnobacterium sp. strain 17-4 Clostridium acetobutylicum strain ATCC 824 Clostridium asparagiforme strain DSM 15981 Clostridium bartlettii strain DSM 16795 Clostridium bolteae strain ATCC BAA-613 Clostridium botulinum A2 strain Kyoto Clostridium butyricum strain 5521 Clostridium cellulovorans strain 743B Clostridium cf. saccharolyticum strain K10 Clostridium citroniae strain WAL-17108 ...
Serpulina pilosicoli is an anaerobic spirochete which has been isolated from the colons of pigs with enteric disease. The clinical and pathologic features of experimental infections of conventional pigs (born by normal farrowing with a naturally acquired intestinal flora) with three strains of S. pilosicoli were determined in order to confirm the enteropathogenicity of this species. Strains were derived from the colons of British pigs with colitis and passaged 8 to 10 times during expansion and purification in vitro. Eighteen ten-week-old Large White-Landrace cross pigs were each inoculated once orally with 0.7 x 10(9) to 1.6 x 10(9) of one of three strains of S. pilosicoli. Six pigs were challenged with each strain. Control pigs were dosed with uninfected broth medium or with 1.8 x 10(7) cells of the nonpathogenic Serpulina innocens. Eight pigs (two to four per S. pilosicoli challenge group) developed soft or diarrheic feces (fecal dry matter | 24%) between 3 and 8 days after challenge, which persisted
Lineage: cellular organisms; Bacteria; Spirochaetes; Spirochaetia; Brachyspirales; Brachyspiraceae; Brachyspira; Brachyspira ...
The anaerobic intestinal spirochaete Brachyspira pilosicoli colonizes the large intestine of various species, including humans. In the colon this spirochaete can penetrate the overlying mucus layer, attach by one cell end to the underlying enterocytes, and initiate localized colitis and diarrhoea. The aim of this study was to investigate whether, as part of the colonization process, B. pilosicoli is attracted to mucin. Fifteen B. pilosicoli strains isolated from humans, pigs, chickens and dogs, and a control strain of Brachyspira hyodysenteriae, were analysed for their ability to enter solutions of hog gastric mucin in an in vitro capillary tube assay. No significant attraction was detected with 1 % mucin, but some strains started to enter a 2 % solution, and attraction then increased with increasing concentrations to peak at around 6-8 % mucin. A similar increase was seen with B. hyodysenteriae, although this activity peaked at 6 % mucin and then declined, suggesting that the two species have different
Strain 56-150T (= DSM 12563 = ATCC 51284 = CIP 105832) is the type strain of the species Brachyspira murdochii. This strain was first described as Serpulina murdochii [1,2], and later transferred to the genus Brachyspira [3]. The genus Brachyspira currently consists of seven species, with Brachyspira aalborgi as the type species [4,5]. The genus Brachyspira is the only genus in the not yet formally described family Brachyspiraceae [6,7]. The generic name derives from brachys, Greek for short, and spira, Latin for a coil, a helix, to mean a short helix [5]. The species name for B. murdochii derives from the city of Murdoch, in recognition of work conducted at Murdoch University in Western Australia, where the type strain was identified [1]. Some species of the genus Brachyspira cause swine dysentery and porcine intestinal spirochetosis. Swine dysentery is a severe, mucohemorrhagic disease that sometimes leads to death of the animals [1]. B. murdochii is generally not considered to be a ...
xml version=1.0 encoding=UTF-8?,,EVENT,,EVENTLOG,,PORTALID,sgm,/PORTALID,,SESSIONID,umSaePaxTlaYuCFfRHW3bvdj.x-sgm-live-02,/SESSIONID,,USERAGENT,CCBot/2.0 (http://commoncrawl.org/faq/),/USERAGENT,,IDENTITYID,guest,/IDENTITYID,,IDENTITY_LIST,guest,/IDENTITY_LIST,,IPADDRESS,107.20.115.174,/IPADDRESS,,EVENTTYPE,PERSONALISATION,/EVENTTYPE,,CREATEDON,1513121663173,/CREATEDON,,/EVENTLOG,,EVENTLOGPROPERTY,,ITEM_ID,http://sgm.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.45597-0,/ITEM_ID,,TYPE,favourite,/TYPE,,/EVENTLOGPROPERTY,,/EVENT ...
Dysentery Definition The term dysentery has been derived from two Greek words meaning ill and intestine. The meaning or definition of dysentery has been provided by medical professionals as an inflammatory disorder that affects the intestines, more specifically the colon and causes high fever, severe diarrhea and
BioAssay record AID 519633 submitted by ChEMBL: Ratio of Kcat to Km for Brachyspira pilosicoli beta-lactamase OXA-63 expressed in Escherichia coli BL21 (DE3) assessed as compound hydrolysis by spectrophotometry.
Synonyms for amebic dysentery in Free Thesaurus. Antonyms for amebic dysentery. 1 synonym for amebic dysentery: amoebic dysentery. What are synonyms for amebic dysentery?
Dysentery: | | Dysentery | | | |ICD|-|10| | | ... World Heritage Encyclopedia, the aggregation of the largest online encyclopedias available, and the most definitive collection ever assembled.
Semantic Scholar extracted view of [Application of media with synthomycine and ribanol to increase the inoculation capacity of dysentery bacteria]. by A I Grekova
There are three ways that our viewers can help us make "The Battle Against Dysentery" a rousing success. The first is to donate - the entire reason we do what we do is because we want to give two big fat checks to our charitable organizations. Every dollar you can spare helps us make those checks fatter. The second is to tell other people, and ask them to donate. Weve always believed that if all you can donate is a dollar, but you tell ten people who also donate a dollar, the kids get $11, and thats some powerful stuff. Third, you can watch the show - sure, were looking for donations, but were also hoping to entertain you. We think that you will really enjoy the show, and we look forward to sharing it with you as much as you do to seeing it every year. ...
Two more people were diagnosed with dysentery in Taichung City following an outbreak of the disease at Beitun Elementary School six days ago.
Learn more about Amoebic Dysentery at Grand Strand Medical Center DefinitionCausesRisk FactorsSymptomsDiagnosisTreatmentPreventionrevision ...
The amoeba that causes dysentery has a unique and gruesome way of attacking the gut: it gnaws away at the walls, ripping off chunks of living cells
In honey bees, dysentery is more of a disorder than a disease. There is no treatment as such but there are several ways to prevent it.
Medication, ORS solutions, and probiotics are some of the options available for amoebic dysentery treatment. Consult a doctor soon, as the disease can turn fatal.
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Mucoid or mucohemorrhagic diarrhea with loss of condition is suggestive. Inflammatory nodules, often with protruding parasites, in mucosa of large intestine. Signs similar to those of swine dysentery and they can occur together. No eggs apparent until 8 weeks post-infection. Early cases diagnosed by histopathology or mucosal scrapings ...
To better understand healthcare use for diarrhoea and dysentery in Nha Trang, Viet Nam, qualitative interviews with community residents and dysentery case studies were conducted. Findings were supplemented by a quantitative survey which asked respondents which healthcare provider their household members would use for diarrhoea or dysentery. A clear pattern of healthcare-seeking behaviours among 433 respondents emerged. More than half of the respondents self-treated initially. Medication for initial treatment was purchased from a pharmacy or with medication stored at home. Traditional home treatments were also widely used. If no improvement occurred or the symptoms were perceived to be severe, individuals would visit a healthcare facility. Private medical practitioners are playing a steadily increasing role in the Vietnamese healthcare system. Less than a quarter of diarrhoea patients initially used government healthcare providers at commune health centres, polyclinics, and hospitals, which are the only
Theres a place in a large city with very bad drinking water, and kids are always getting sick with dysentery. So you keep treating the dysentery, but meanwhile it would be much better to clean up the drinking water... The drugs work on the dysentery for about 48 hours, but youre NOT treating the problem. And the problem is NOT genetic. ...
Also called: Dysentery, The runs, The trots Diarrhea means that you have loose, watery stools more than three times in one day. You may also have c...
The term intestinal spirochetosis (IS) was coined in 1967 for an infection of the large bowel in which uncharacterized spirochetes were found attached by one end to the colonic epithelium to form a dense "false brush border" (11). Subsequently, there has been considerable controversy over the pathologic and epidemiologic significance of this colonization. Some investigators have reported symptoms such as rectal bleeding and/or diarrhea in patients with IS (6, 7, 9, 20, 30, 42), and in some cases clinical improvement has occurred after treatment with antibiotics which eliminated the spirochetes (7, 14, 29). Other investigators have been unable to relate the presence of intestinal spirochetes to gastrointestinal symptoms (3, 26, 31, 32).. Most studies of IS have involved histologic examination of biopsy material, without concurrent bacterial culture (17, 21, 24, 31, 42); a smaller number of studies have involved only fecal culture for spirochetes (3, 13, 18, 30, 33). One exception was a study by ...
Amoeba Dysentery directory ☆ Amoeba Dysentery manufacturers, suppliers ☆ Amoeba Dysentery buyers, importers, wholesalers, distributors
Thule Trail takes the classic Oregon Trail formula, strips out the wagon, strips out the dysentery, gives you a car with Thule gear, and creates an amazingly entertaining Flash game.. Road trip to the Atlantis Music Festival!. See if you have what it takes to navigate through nasty weather, road grub, car problems, and crazy drifters. There are plenty of mini-games to keep you entertained, a dry humor that will make you chuckle, and theyve even used 8-bit sound for extra authenticity.. Can you make it to Santa Barbara?. [Thule Trail]. [Via: Kotaku]. ...
Doctors for Breast Reconstruction in Delhi. Find Doctors Near You, Book Doctors Appointment, Consult Online, View Cost for Breast Reconstruction in Delhi | Lybrate
Dysentery is an intestinal inflammation, It is a type of gastroenteritis that results in diarrhea with blood. It can lead to mild or severe stomach cramps and severe diarrhea with mucus or blood in the feces. The mechanism is an inflammatory disorder of the intestine, especially of the colon.. Causes of Dysentery. It is caused by several types of infections such as bacteria, viruses, parasitic worms, or protozoa.. ...
inflammatory disorder of the intestine, usually caused by a bacterial, parasitic, or protozoan infection and resulting in pain, fever, and severe diarrhea, often accompanied by the frequent passage of feces, usually with blood and mucus. Dysentery is spread in food or water contaminated by feces. ...
Dabur Kutajghan Vati Sidhyog Sangraha Ayurvedic Treatment for Diarrhoea and Dysentery. Dabur Health Care Herbal Products in India. Online Dabur Ayurvedic Medicines.
My husband was diagnosed of dysentery pile in 2013 and it was really tough and heartbreaking for me because he was my all and the symptoms were terrible, he had difficulty eating, and he always complain of stomach pain. we tried various therapies prescribed by our neurologist but none could cure him. I searched for a cure and i saw a testimony by someone who was cured and so many other with similar body problem, and he left the contact of the doctor who had the cure to dysentery pile. I never imagined dysentery pile has a cure not until i contacted him and he assured me my husband will be fine. I got the herbal medication he recommended and my husband used it and in one months he was fully okay even up till this moment he is so full of life. dysentery pile has a cure and it is a herbal cure contact the doctor for more info on [email protected] on how to get the medication. Thanks for reading my story. Delete ...
Antidysenteric serum can be safely prepared in the horse by the method of three successive intravenous injections of living cultures or toxin with intervening rest periods of seven days.. When this method of immunization is employed, the specific antibodies responsible for agglutination and protection appear early and rise rapidly.. By inoculating alternately living dysentery bacilli belonging to the Shiga and Flexner groups a polyvalent serum of high titer may be prepared.. A polyvalent serum so produced should be suitable for the therapeutic treatment of acute bacillary dysentery, irrespective of the particular strain or strains of the dysentery bacillus causing the infection.. An effective antidysentery serum suitable for therapeutic employment in man can be prepared in the horse in about ten weeks.. ...
... and proposals of Brachyspira hyodysenteriae Comb. Nov., Brachyspira innocens Comb. Nov. and Brachyspira pilosicoli Comb. Nov". ... Both species however are classified as belonging to the genus Brachyspira, together with Brachyspira pilosicoli. They are ... nov., as Serpula hyodysenteriae comb. nov. and Serpula innocens comb. nov". International Journal of Systematic Bacteriology. ... Containing the Species Serpulina hyodysenteriae comb. nov. and Serpulina innocens comb. nov". International Journal of ...
... hyodysenteriae or Brachyspira pilosicoli by PCR. Brachyspira species previously capable of weak hemolysis only, like B. ... Antimicrobial susceptibility of porcine Brachyspira hyodysenteriae and Brachyspira pilosicoli isolated in Sweden between 1990 ... B. hyodysenteriae and B. pilosicoli are the classic Brachyspira gastrointestinal pathogens leading to diarrheal disease in ... Zmudzki J, Szczotka A, Nowak A, Strzelecka H, Grzesiak A, Pejsak Z.Antimicrobial susceptibility of Brachyspira hyodysenteriae ...
... , formerly known as Brachyspira hyodysenteriae and Treponema hyodysenteriae, is a species of bacteria. ... Harris DL; Glock RD; Christensen CR; Kinyon JM (January 1972). "Inoculation of pigs with Treponema hyodysenteriae (new species ...
The Brachyspira holin (B-Hol) Family (TC# 1.E.55) consists of several proteins from the GTA holin of Brachyspira hyodysenteriae ... a prophage-like gene transfer agent of Brachyspira hyodysenteriae". Journal of Bacteriology 187 (17): 5885-5892. doi:10.1128/JB ... Molecular and Cellular Biology portal As of this edit, this article uses content from "1.E.55 The Brachyspira holin (B-Hol) ... "1.E.55 The Brachyspira holin (B-Hol) Family". TCDB. Retrieved 2016-03-29. ...
For swine dysentery, which is caused by Brachyspira hyodysenteriae infection and is an economically important disease in ... For swine dysentery, which is caused by Brachyspira hyodysenteriae infection and is an economically important disease in ... Estimating diagnostic test accuracies for brachyspira hyodysenteriae accounting for the complexities of population structure in ... Estimating diagnostic test accuracies for brachyspira hyodysenteriae accounting for the complexities of population structure in ...
... of diets with contrasting fermentability in the large intestine on experimental infections with Brachyspira hyodysenteriae, the ... Pigs fed Diet 2 and challenged with B. hyodysenteriae did not develop swine dysentery and B. hyodysenteriae was not ... In contrast, 94% of the B. hyodysenteriae challenged pigs fed Diet I showed clinical symptoms of swine dysentery and all the ... hyodysenteriae only. The study showed that diets supplemented with highly fermentable carbohydrates from dried chicory roots ...
Fifteen B. pilosicoli strains isolated from humans, pigs, chickens and dogs, and a control strain of Brachyspira hyodysenteriae ... B. hyodysenteriae B204 showed a similar viscotaxis to PVP. Further studies are required to determine whether the in vitro ... A similar increase was seen with B. hyodysenteriae, although this activity peaked at 6 % mucin and then declined, suggesting ... These mucin concentrations were much higher than those used in previous experimental studies with Brachyspira species. The ...
Identification of Brachyspira hyodysenteriae and other pathogenic Brachyspira species in chickens from laying flocks with ... Hampson DJ and La T. Reclassification of Serpulina intermedia and Serpulina murdochii in the genus Brachyspira as Brachyspira ... with Brachyspira aalborgi as the type species [4,5]. The genus Brachyspira is the only genus in the not yet formally described ... Genome sequence of the pathogenic intestinal spirochaete Brachyspira hyodysenteriae reveals adapations to its lifestyle in the ...
Methane metabolism - Brachyspira hyodysenteriae WA1 [ Pathway menu , Organism menu , Pathway entry , Download KGML , Show ...
The most common aetiological agent is the anaerobic intestinal spirochaete Brachyspira hyodysenteriae.The related... ... Identification of Brachyspira hyodysenteriae and other pathogenic Brachyspira species in chickens from laying flocks with ... Development of a duplex PCR assay for the detection of Brachyspira hyodysenteriae and Brachyspira pilosicoli in pig feces. J ... although in some regions the related species Brachyspira hampsonii and Brachyspira suanatina also may cause SD [2]. Brachyspira ...
... being Brachyspira intermedia, Brachyspira pilosicoli, Brachyspira alvinipulli and Brachyspira hyodysenteriae, and one or more ... Diagnóstico de Brachyspira pilosicoli, Brachyspira hyodysenteriae e Brachyspira intermedia em aves de postura e matrizes de ... Diagnosis of Brachyspira pilosicoli, Brachyspira hyodysenteriae and Brachyspira intermedia in hens and laying hens in the ... INDEX TERMS: Brachyspira pilosicoli; Brachyspira hyodysenteriae; Brachyspira intermedia; hens; laying hens; Paraná; bacterial ...
Brachyspira (Serpulina)hyodysenteriae, the etiologic agent of swine dysentery, uses the enzyme NADH oxidase to consume oxygen. ... Brachyspira(Serpulina) hyodysenteriae cells colonize the oxygen-respiring mucosal tissues of the swine cecum and colon. During ... Results with B. hyodysenteriaeB204 (wild type) (A) and B. hyodysenteriae Nox-Km (nox mutant) (B) are shown. Two-microliter ... Derivation of B. hyodysenteriae nox mutants.Transformation of B. hyodysenteriae B204 cells by electroporation was based on ...
... hyodysenteriae farm strains. This study highlights the potential role of shipments in B. hyodysenteriae spread. Moreover, it ... The objective of this study was to investigate the role of slaughterhouse vehicles in spreading B. hyodysenteriae between ... hyodysenteriae genetic profiles. STVT 83-3, which seems to be the current dominant type in Italy, was identified in 56.25% of ... hyodysenteriae, followed by categories 4 and 3 with 85.7% and 83.3%, respectively. The results of MLST and MLVA suggest that ...
Changes in the content of neuropeptides in intestinal lymph nodes of pigs suffering from experimental Brachyspira ... hyodysenteriae infection , M. Lakomy, A. Winnicka, K. Wasowicz, J. Zmudzki, J. Kaleczyc, W. Sienkiewicz, P. Podlasz , ... in 4-months old pigs in which experimental enteritis was induced with Brachyspira hyodysenteriae infection, a statistically ... in the content of neuropeptides in intestinal lymph nodes of pigs suffering from experimental Brachyspira hyodysenteriae ...
Brachyspira hyodysenteriae, Brachyspira hyodysenteriae WA1, Brachyspira hyodysenteriae str. WA1, Brachyspira hyodysenteriae ... Brachyspira hyodysenteriae. NCBI taxonomy Id: 565034. Other names: B. hyodysenteriae WA1, ...
Antimicrobial susceptibility testing of Australian isolates of Brachyspira hyodysenteriae using a new broth dilution method ... The antimicrobial susceptibilities of 76 field isolates of Brachyspira hyodysenteriae from different states of Australia were ... 2002) Antimicrobial susceptibility testing of Australian isolates of Brachyspira hyodysenteriae using a new broth dilution ...
Animals , Swine Diseases/diagnosis , Gram-Negative Bacterial Infections , Brachyspira hyodysenteriae , Brachyspira , Sus scrofa ... Brachyspira hyodysenteriae / Brachyspira / Sus scrofa / Dysentery Clinical aspect: Diagnosis Limits: Animals Language: ... Disenteria Suína e Colite Espiroquetal são duas enfermidades importantes em suínos causados pela Brachyspira hyodysenteriae e ... Hibridização in situ fluorescente para diagnóstico de Brachyspira hyodysenteriae e B. pilosicoli -em suínos / Fluorescence in ...
Virulence of strong and weakly haemolytic strains of Brachyspira hyodysenteriae. . Biblioteca virtual para leer y descargar ... Virulence of strong and weakly haemolytic strains of Brachyspira hyodysenteriae - Descarga este documento en PDF. Documentación ...
Except for Brachyspira hyodysenteriae, the clinical significance of these in vitro activities is unknown. ... Tiamulin is active against Brachyspira hyodysenteriae. The antibacterial activity of tiamulin is due to its inhibitory effects ... Denagard Injection is effective for treatment of swine dysentery associated with Brachyspira hyodysenteriae susceptible to ...
Identification of the gene encoding BmpB, a 30 kDa outer envelope lipoprotein of Brachyspira (Serpulina) hyodysenteriae, and ... 2000) Identification of the gene encoding BmpB, a 30 kDa outer envelope lipoprotein of Brachyspira (Serpulina) hyodysenteriae, ... A gene encoding a 30 kDa outer envelope protein of the intestinal spirochaete Brachyspira (Serpulina) hyodysenteriae, was ... Sera from these mice and pigs recognised the 30 kDa lipoprotein in outer membrane preparations of B. hyodysenteriae, indicating ...
All eight genes previously associated with haemolysis in B. hyodysenteriae were present in the whBh. No consistent patterns of ... Typical strains of B. hyodysenteriae are strongly haemolytic on blood agar, and the haemolytic activity is believed to ... However, recently there have been reports of atypical weakly haemolytic isolates of B. hyodysenteriae (whBh). In this study, 34 ... hyodysenteriae. Another difference in this region was the presence of three CDSs in whBh that are pseudogenes in strongly ...
We evaluated the susceptibility of B. hyodysenteriae, B. hampsonii, Brachyspira pilosicoli, and Brachyspira murdochii to ... Outbreaks of swine dysentery, caused by Brachyspira hyodysenteriae and the recently discovered "Brachyspira hampsonii," have ... Antimicrobial susceptibility of porcine Brachyspira hyodysenteriae and Brachyspira pilosicoli isolated in Sweden between 1990 ... Multiresistant Brachyspira hyodysenteriae in a Dutch sow herd. Tijdschr Diergeneeskd 133:604-608. (In Dutch.). ...
An update of Brachyspira hyodysenteriae serotyping.. Res Vet Sci. 2017 111: 135-139.. Herbst W, Willems H. (2017). Detection of ... Phylogenetic diversity, antimicrobial susceptibility and virulence gene profiles of Brachyspira hyodysenteriae isolates from ... Isolation and antimicrobial susceptibility of Brachyspira species from feces of layer chickens in Germany. Tierarztl Prax Ausg ...
A water extract of Thyme inhibited Brachyspira hyodysenteriae, the causative agent of swine dysentery.Nov 30, 2016. ...
Genes and proteins of brachyspira hyodysenteriae and uses thereof. EP3147365A1. Apr 20, 2007. Mar 29, 2017. Intervet ... Genes and proteins of brachyspira hyodysenteriae and use of same for diagnosis and therapy. ... Sequences of brachyspira, immunogenic composition, methods for preparation and use thereof. EP2636743A1. Apr 20, 2007. Sep 11, ... Novel sequences of brachyspira, immunogenic compositions, methods for preparation and use thereof. ...
Brachyspira contains Hemolysin. The more ____ the bacteria, the more chance that it will be associated with bad disease ... What virulence factor is necessary for Brachyspira and allows the bacteria to move through intestinal mucosa? ... What are the differentials for Brachyspira Hyodysenteriea that are hard to differentiate? ... The more virulent the strain of Brachyspira, the more ____ on blood agar ...
Brachyspira hyodysenteriae ‎ *15:35, 13 August 2013 (diff , hist) . . (+14)‎ . . Body Odor ‎ (current) ...
Virulence of strong and weakly haemolytic strains of Brachyspira hyodysenteriae Maxime Mahu (UGent) , Nele De Pauw (UGent) , ... In vitro susceptibility of Brachyspira hyodysenteriae to organic acids and essential oil components Lien Vande Maele (UGent) , ... In vitro sensitivity of Brachyspira hyodysenteriae to organic acids and essential oil components Lien Vande Maele (UGent) , ... swine dysentery, brachyspira hyodysenteriae, swine-dysentery, swine, resistance, pigs, intermedia, identification, haemolysis, ...
Brachyspira hyodysenteriae ‎ (current) *15:13, 22 February 2016 (diff , hist) . . (+3)‎ . . Bifidobacterium breve ‎ (current) ...
  • For swine dysentery, which is caused by Brachyspira hyodysenteriae infection and is an economically important disease in intensive pig production systems worldwide, a perfect or error-free diagnostic test (''gold standard'') is not available. (uzh.ch)
  • The aim of the present study was to estimate test accuracies of a PCR originally designed for use as a confirmatory test, displaying a high diagnostic specificity, and cultural examination for B. hyodysenteriae. (uzh.ch)
  • These results provide evidence that NADH oxidase serves to protect B. hyodysenteriae cells against oxygen toxicity and that the enzyme, in that role, contributes to the pathogenic ability of the spirochete. (asm.org)
  • The enzyme may be important in early stages of the disease when cells first populate mucosal tissues or in later stages when oxygen-carrying erythrocytes enter the spirochete habitat and are possibly lysed by the B. hyodysenteriae hemolysin. (asm.org)
  • The essential causal agent is Brachyspira hyodysenteriae , an anaerobic spirochete that produces a hemolysin, although other organisms may contribute to the severity of lesions. (merckvetmanual.com)
  • For the most part, bacterial characteristics essential for B. hyodysenteriae colonization and pathogenesis have not been thoroughly investigated, although there is evidence that hemolytic activity ( 15 , 48 ) and bacterial motility and chemotaxis ( 18 , 26 , 32 ) are important contributing factors. (asm.org)
  • Additionally, since the latter part of 2008, there has been a concurrent increase in the number of cases with diarrhoea and lesions suggestive of SD from which a strongly β-haemolytic spirochaete was isolated that was not identified as B. hyodysenteriae by available laboratory methods (Figure 1). (thepigsite.com)
  • Brachyspira hyodysenteriae was isolated from two faecal samples from 14-week-old pigs with mucoid diarrhoea and wasting in the Thirsk region. (thepigsite.com)
  • The study had a three-factorial design, with eight groups of pigs receiving Diet I or Diet 2, +/- B. hyodysenteriae, and +/- T suis. (dtu.dk)
  • For the treatment and control of SD associated with Brachyspira hyodysenteriae when followed immediately by tylosin phosphate Type A medicated article in feed. (drugs.com)
  • The purified NADH oxidase of B. hyodysenteriae B204 is a flavin adenine dinucleotide-dependent, monomeric protein with an apparent molecular mass, based on gel migration, of 47 to 48 kDa ( 45 ). (asm.org)
  • Brachyspira hyodysenteriae colonizes the pig colon, resulting in mucoid hemorrhagic diarrhea and mucus layer changes. (gu.se)