Bovine papillomavirus 1: A species of DELTAPAPILLOMAVIRUS infecting cattle.Bovine papillomavirus 4: A type of XIPAPILLOMAVIRUS causing alimentary carcinoma in cattle. It is related to Bovine papillomavirus 3.Papillomavirus Infections: Neoplasms of the skin and mucous membranes caused by papillomaviruses. They are usually benign but some have a high risk for malignant progression.Papillomaviridae: A family of small, non-enveloped DNA viruses infecting birds and most mammals, especially humans. They are grouped into multiple genera, but the viruses are highly host-species specific and tissue-restricted. They are commonly divided into hundreds of papillomavirus "types", each with specific gene function and gene control regions, despite sequence homology. Human papillomaviruses are found in the genera ALPHAPAPILLOMAVIRUS; BETAPAPILLOMAVIRUS; GAMMAPAPILLOMAVIRUS; and MUPAPILLOMAVIRUS.Oncogene Proteins, Viral: Products of viral oncogenes, most commonly retroviral oncogenes. They usually have transforming and often protein kinase activities.Human papillomavirus 16: A type of ALPHAPAPILLOMAVIRUS especially associated with malignant tumors of the CERVIX and the RESPIRATORY MUCOSA.Papillomavirus E7 Proteins: ONCOGENE PROTEINS from papillomavirus that deregulate the CELL CYCLE of infected cells and lead to NEOPLASTIC CELL TRANSFORMATION. Papillomavirus E7 proteins have been shown to interact with various regulators of the cell cycle including RETINOBLASTOMA PROTEIN and certain cyclin-dependent kinase inhibitors.Papillomavirus Vaccines: Vaccines or candidate vaccines used to prevent PAPILLOMAVIRUS INFECTIONS. Human vaccines are intended to reduce the incidence of UTERINE CERVICAL NEOPLASMS, so they are sometimes considered a type of CANCER VACCINES. They are often composed of CAPSID PROTEINS, especially L1 protein, from various types of ALPHAPAPILLOMAVIRUS.DNA, Viral: Deoxyribonucleic acid that makes up the genetic material of viruses.Human papillomavirus 18: A type of human papillomavirus especially associated with malignant tumors of the genital and RESPIRATORY MUCOSA.Tumor Virus Infections: Infections produced by oncogenic viruses. The infections caused by DNA viruses are less numerous but more diverse than those caused by the RNA oncogenic viruses.Human papillomavirus 11: A type of ALPHAPAPILLOMAVIRUS causing recurrent respiratory PAPILLOMATOSIS; GENITAL WARTS; and other neoplasms.Uterine Cervical Neoplasms: Tumors or cancer of the UTERINE CERVIX.Viral Proteins: Proteins found in any species of virus.Cell Transformation, Viral: An inheritable change in cells manifested by changes in cell division and growth and alterations in cell surface properties. It is induced by infection with a transforming virus.Papilloma: A circumscribed benign epithelial tumor projecting from the surrounding surface; more precisely, a benign epithelial neoplasm consisting of villous or arborescent outgrowths of fibrovascular stroma covered by neoplastic cells. (Stedman, 25th ed)Cottontail rabbit papillomavirus: The type species of KAPPAPAPILLOMAVIRUS. It is reported to occur naturally in cottontail rabbits in North America.Deltapapillomavirus: A genus of DNA viruses in the family PAPILLOMAVIRIDAE causing fibropapillomas in their respective ungulate hosts. Species infected include cattle, European elk, deer, and sheep.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Warts: Benign epidermal proliferations or tumors; some are viral in origin.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.Genes, Viral: The functional hereditary units of VIRUSES.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Alphapapillomavirus: A genus of DNA viruses in the family PAPILLOMAVIRIDAE. They preferentially infect the anogenital and ORAL MUCOSA in humans and primates, causing both malignant and benign neoplasms. Cutaneous lesions are also seen.Human papillomavirus 6: A type of ALPHAPAPILLOMAVIRUS usually associated with GENITAL WARTS; and LARYNGEAL NEOPLASMS.Gene Expression Regulation, Viral: Any of the processes by which cytoplasmic factors influence the differential control of gene action in viruses.Cell Line: Established cell cultures that have the potential to propagate indefinitely.DNA Replication: The process by which a DNA molecule is duplicated.Cattle Diseases: Diseases of domestic cattle of the genus Bos. It includes diseases of cows, yaks, and zebus.Cervical Intraepithelial Neoplasia: A malignancy arising in uterine cervical epithelium and confined thereto, representing a continuum of histological changes ranging from well-differentiated CIN 1 (formerly, mild dysplasia) to severe dysplasia/carcinoma in situ, CIN 3. The lesion arises at the squamocolumnar cell junction at the transformation zone of the endocervical canal, with a variable tendency to develop invasive epidermoid carcinoma, a tendency that is enhanced by concomitant human papillomaviral infection. (Segen, Dictionary of Modern Medicine, 1992)Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Horse Diseases: Diseases of domestic and wild horses of the species Equus caballus.Capsid Proteins: Proteins that form the CAPSID of VIRUSES.Condylomata Acuminata: Sexually transmitted form of anogenital warty growth caused by the human papillomaviruses.Human papillomavirus 31: A type of ALPHAPAPILLOMAVIRUS associated with high risk for anogenital neoplasms.Cervix Uteri: The neck portion of the UTERUS between the lower isthmus and the VAGINA forming the cervical canal.Cell Line, Transformed: Eukaryotic cell line obtained in a quiescent or stationary phase which undergoes conversion to a state of unregulated growth in culture, resembling an in vitro tumor. It occurs spontaneously or through interaction with viruses, oncogenes, radiation, or drugs/chemicals.Virus Replication: The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.Receptor, Platelet-Derived Growth Factor beta: A PDGF receptor that binds specifically to the PDGF-B chain. It contains a protein-tyrosine kinase activity that is involved in SIGNAL TRANSDUCTION.Vaginal Smears: Collection of pooled secretions of the posterior vaginal fornix for cytologic examination.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Replication Origin: A unique DNA sequence of a replicon at which DNA REPLICATION is initiated and proceeds bidirectionally or unidirectionally. It contains the sites where the first separation of the complementary strands occurs, a primer RNA is synthesized, and the switch from primer RNA to DNA synthesis takes place. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)Enhancer Elements, Genetic: Cis-acting DNA sequences which can increase transcription of genes. Enhancers can usually function in either orientation and at various distances from a promoter.Repressor Proteins: Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Cell Transformation, Neoplastic: Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.Keratinocytes: Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.DNA Probes, HPV: DNA probes specific for the identification of human papilloma virus.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Genome, Viral: The complete genetic complement contained in a DNA or RNA molecule in a virus.Capsid: The outer protein protective shell of a virus, which protects the viral nucleic acid.Uterine Cervical Diseases: Pathological processes of the UTERINE CERVIX.Recombinant Fusion Proteins: Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.Adenovirus E2 Proteins: Proteins transcribed from the E2 region of ADENOVIRUSES. Several of these are required for viral DNA replication.Buffaloes: Ruminants of the family Bovidae consisting of Bubalus arnee and Syncerus caffer. This concept is differentiated from BISON, which refers to Bison bison and Bison bonasus.Transfection: The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.Horses: Large, hoofed mammals of the family EQUIDAE. Horses are active day and night with most of the day spent seeking and consuming food. Feeding peaks occur in the early morning and late afternoon, and there are several daily periods of rest.Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Receptors, Platelet-Derived Growth Factor: Specific receptors on cell membranes that react with PLATELET-DERIVED GROWTH FACTOR, its analogs, or antagonists. The alpha PDGF receptor (RECEPTOR, PLATELET-DERIVED GROWTH FACTOR ALPHA) and the beta PDGF receptor (RECEPTOR, PLATELET-DERIVED GROWTH FACTOR BETA) are the two principle types of PDGF receptors. Activation of the protein-tyrosine kinase activity of the receptors occurs by ligand-induced dimerization or heterodimerization of PDGF receptor types.Equidae: A family of hoofed MAMMALS consisting of HORSES, donkeys, and zebras. Members of this family are strict herbivores and can be classified as either browsers or grazers depending on how they feed.Open Reading Frames: A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Antibodies, Viral: Immunoglobulins produced in response to VIRAL ANTIGENS.Virion: The infective system of a virus, composed of the viral genome, a protein core, and a protein coat called a capsid, which may be naked or enclosed in a lipoprotein envelope called the peplos.Human Papillomavirus DNA Tests: Methods for detecting or typing the DNA of an ALPHAPAPILLOMAVIRUS in biological tissues and fluids.Nucleic Acid Hybridization: Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)RNA, Viral: Ribonucleic acid that makes up the genetic material of viruses.Trans-Activators: Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Restriction Mapping: Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.Uterine Cervical Dysplasia: Abnormal development of immature squamous EPITHELIAL CELLS of the UTERINE CERVIX, a term used to describe premalignant cytological changes in the cervical EPITHELIUM. These atypical cells do not penetrate the epithelial BASEMENT MEMBRANE.Penile Neoplasms: Cancers or tumors of the PENIS or of its component tissues.Papanicolaou Test: Cytological preparation of cells collected from a mucosal surface and stained with Papanicolaou stain.Vulvar Neoplasms: Tumors or cancer of the VULVA.Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.Transcriptional Activation: Processes that stimulate the GENETIC TRANSCRIPTION of a gene or set of genes.Serum Albumin, Bovine: Serum albumin from cows, commonly used in in vitro biological studies. (From Stedman, 25th ed)HeLa Cells: The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.Viral Vaccines: Suspensions of attenuated or killed viruses administered for the prevention or treatment of infectious viral disease.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Herpesvirus 1, Bovine: A species of VARICELLOVIRUS that causes INFECTIOUS BOVINE RHINOTRACHEITIS and other associated syndromes in CATTLE.Carcinoma, Squamous Cell: A carcinoma derived from stratified SQUAMOUS EPITHELIAL CELLS. It may also occur in sites where glandular or columnar epithelium is normally present. (From Stedman, 25th ed)Genetic Vectors: DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Sarcoidosis: An idiopathic systemic inflammatory granulomatous disorder comprised of epithelioid and multinucleated giant cells with little necrosis. It usually invades the lungs with fibrosis and may also involve lymph nodes, skin, liver, spleen, eyes, phalangeal bones, and parotid glands.Oncogenes: Genes whose gain-of-function alterations lead to NEOPLASTIC CELL TRANSFORMATION. They include, for example, genes for activators or stimulators of CELL PROLIFERATION such as growth factors, growth factor receptors, protein kinases, signal transducers, nuclear phosphoproteins, and transcription factors. A prefix of "v-" before oncogene symbols indicates oncogenes captured and transmitted by RETROVIRUSES; the prefix "c-" before the gene symbol of an oncogene indicates it is the cellular homolog (PROTO-ONCOGENES) of a v-oncogene.Oropharyngeal Neoplasms: Tumors or cancer of the OROPHARYNX.Anus Neoplasms: Tumors or cancer of the ANAL CANAL.Colposcopy: The examination, therapy or surgery of the cervix and vagina by means of a specially designed endoscope introduced vaginally.Regulatory Sequences, Nucleic Acid: Nucleic acid sequences involved in regulating the expression of genes.Epidermodysplasia Verruciformis: An autosomal recessive trait with impaired cell-mediated immunity. About 15 human papillomaviruses are implicated in associated infection, four of which lead to skin neoplasms. The disease begins in childhood with red papules and later spreads over the body as gray or yellow scales.Genes, Regulator: Genes which regulate or circumscribe the activity of other genes; specifically, genes which code for PROTEINS or RNAs which have GENE EXPRESSION REGULATION functions.Cricetinae: A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.Gammapapillomavirus: A genus of DNA viruses in the family PAPILLOMAVIRIDAE, which cause cutaneous lesions in humans. They are histologically distinguishable by intracytoplasmic INCLUSION BODIES which are species specific.Betapapillomavirus: A genus of DNA viruses in the family PAPILLOMAVIRIDAE, causing cutaneous lesions in humans. Infections exist in latent form in the general population and are activated under conditions of IMMUNOSUPPRESSION.Neutralization Tests: The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).COS Cells: CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)Mutagenesis: Process of generating a genetic MUTATION. It may occur spontaneously or be induced by MUTAGENS.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.DNA, Recombinant: Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.Skin Neoplasms: Tumors or cancer of the SKIN.Blotting, Southern: A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.DNA Primers: Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.DNA Restriction Enzymes: Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.Vaginal Neoplasms: Tumors or cancer of the VAGINA.Fibroblasts: Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.DNA Mutational Analysis: Biochemical identification of mutational changes in a nucleotide sequence.Tonsillar Neoplasms: Tumors or cancer of the PALATINE TONSIL.CHO Cells: CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.3T3 Cells: Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.DNA Helicases: Proteins that catalyze the unwinding of duplex DNA during replication by binding cooperatively to single-stranded regions of DNA or to short regions of duplex DNA that are undergoing transient opening. In addition DNA helicases are DNA-dependent ATPases that harness the free energy of ATP hydrolysis to translocate DNA strands.Simian virus 40: A species of POLYOMAVIRUS originally isolated from Rhesus monkey kidney tissue. It produces malignancy in human and newborn hamster kidney cell cultures.Mutagenesis, Site-Directed: Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.Leukemia Virus, Bovine: The type species of DELTARETROVIRUS that causes a form of bovine lymphosarcoma (ENZOOTIC BOVINE LEUKOSIS) or persistent lymphocytosis.Viral Structural Proteins: Viral proteins that are components of the mature assembled VIRUS PARTICLES. They may include nucleocapsid core proteins (gag proteins), enzymes packaged within the virus particle (pol proteins), and membrane components (env proteins). These do not include the proteins encoded in the VIRAL GENOME that are produced in infected cells but which are not packaged in the mature virus particle,i.e. the so called non-structural proteins (VIRAL NONSTRUCTURAL PROTEINS).Cell Nucleus: Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Epitopes: Sites on an antigen that interact with specific antibodies.

Induction of autoantibodies to mouse CCR5 with recombinant papillomavirus particles. (1/561)

The vertebrate immune system has evolved to respond vigorously to microbial infection but to ignore self-antigens. Evidence has emerged that B cell responses to viruses are initiated by immune recognition of ordered arrays of antigen on the viral surface. To test whether autoantibodies against a self-antigen can be induced by placing it in a context that mimics the ordered surface of a viral particle, a peptide representing an extracellular loop of the mouse chemokine receptor CCR5 was incorporated into an immunodominant site of the bovine papillomavirus virus L1 coat protein, which self-assembles into virus-like particles. Mice inoculated with chimeric L1-CCR5 particles generated autoantibodies that bound to native mouse CCR5, inhibited binding of its ligand RANTES, and blocked HIV-1 infection of an indicator cell line expressing a human-mouse CCR5 chimera. These results suggest a general method for inducing autoantibodies against self-antigens, with diverse potential basic research and clinical applications.  (+info)

A mutational analysis of the transforming functions of the E8 protein of bovine papillomavirus type 4. (2/561)

The E8 protein of BPV-4 contributes to transformation of primary bovine cells (PalFs) by inducing anchorage-independent growth and by down-regulating gap junction intercellular communication, likely due to its binding to 16K ductin. We show here that, in addition, E8 confers on PalF cells the ability to grow in low serum and to escape from contact inhibition (focus formation). E8 also transactivates an exogenous human cyclin A gene promoter, suggesting that overexpression of cyclin A is responsible for the transformed phenotype. Mutant forms of E8 were generated to establish whether the transforming functions of the protein could be segregated. Mutations were introduced both in the hydrophobic domain and in the hydrophilic C-terminal "tail", and chimeras with BPV-1 E5 were constructed. Cells expressing either wild-type E8 or mutant forms were analyzed for their ability to grow in low serum and in suspension and to form foci. Wild-type E8 and its mutants were also analyzed for their ability to transactivate the cyclin A promoter. We show here that the transforming functions of E8 can be segregated and that both the hydrophilic C-terminal tail and the residue at position 17 in the hydrophobic domain are crucial for E8 functions and for the transactivation of the cyclin A promoter. These results support the hypothesis that the different aspects of cellular transformation brought about by E8 might be due to interaction with different cellular targets. They suggest that E8 might function differently from BPV-1 E5 and demonstrate that the separate domains of E5 and E8 are not functionally interchangeable.  (+info)

Long-term episomal maintenance of bovine papillomavirus type 1 plasmids is determined by attachment to host chromosomes, which Is mediated by the viral E2 protein and its binding sites. (3/561)

Papillomavirus genomes are stably maintained as extrachromosomal nuclear plasmids in dividing host cells. To address the mechanisms responsible for stable maintenance of virus, we examined nuclear compartmentalization of plasmids containing the full-length upstream regulatory region (URR) from the bovine papillomavirus type 1 (BPV1) genome. We found that these plasmids are tightly associated with the nuclear chromatin both in the stable cell lines that maintain episomal copies of the plasmids and in transiently transfected cells expressing the viral E1 and E2 proteins. Further analysis of viral factors revealed that the E2 protein in trans and its multiple binding sites in cis are both necessary and sufficient for the chromatin attachment of the plasmids. On the other hand, the BPV1 URR-dependent plasmid replication and chromatin attachment processes are clearly independent of each other. The ability of the plasmids to stably maintain episomes correlates clearly with their chromatin association function. These data suggest that viral E2 protein-mediated attachment of BPV1 genomes to the host cell chromatin could provide a mechanism for the coupling of viral genome multiplication and partitioning to the host cell cycle during viral latent infection.  (+info)

Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication. (4/561)

The bovine papillomavirus type 1 (BPV-1) E2 protein is the master regulator of papillomavirus replication and transcription. We have raised a panel of monoclonal antibodies (MAbs) against the BPV-1 E2 protein and used them to probe the structure and function of the protein. Five MAbs reacted with linear epitopes, and four MAbs recognized conformation-dependent epitopes which mapped within the C-terminal DNA-binding and dimerization domain. MAb 1E2 was able to recognize the replication- and transactivation-defective but not the competent conformation of the transactivation domain of the E2 protein. MAb 5H4 prevented efficiently the formation of E2-DNA as well as E2-dependent E1-E2-origin complexes and also dissociated preformed complexes in a concentration-dependent manner. Cotransfection of several MAbs with the BPV-1 minimal origin plasmid pUCAlu into CHO4.15 cells resulted in a dose-dependent inhibition of replication. Inhibition of replication by MAb 5H4 and the Fab' fragment of 5H4 correlated with their ability to dissociate the E2 protein from the DNA. MAb 3F12 and MAbs 1H10 and 1E4, directed against the hinge region, were also capable of inhibiting BPV-1 origin replication in CHO4.15 cells. However, the Fab' fragments of 1H10 and 3F12 had no effect in the transient replication assay. These data suggest that MAbs directed against the hinge region sterically hinder the inter- or intramolecular interactions required for the replication activity of the E2 protein.  (+info)

Papillomavirus capsid protein expression level depends on the match between codon usage and tRNA availability. (5/561)

Translation of mRNA encoding the L1 and L2 capsid proteins of papillomavirus (PV) is restricted in vivo to differentiated epithelial cells, although transcription of the L1 and L2 late genes occurs more widely. The codon composition of PV late genes is quite different from that of most mammalian genes. To test the possibility that PV late gene codon composition determines the efficiency of PV late gene expression in some cell types, synthetic bovine papillomavirus type 1 (BPV1) late genes were constructed with codon composition modified to resemble the typical mammalian gene. Expression of these genes from a strong promoter in Cos-1 cells was compared with expression of wild-type BPV1 late genes from the same promoter. Both unmodified and modified PV late genes were transcribed in Cos-1 cells, but only the codon-modified genes were translated. In vitro translation of wild-type but not synthetic BPV1 L1 mRNA was markedly enhanced by addition of aminoacyl-tRNAs. Codon composition thus limits BPV1 late gene translation in Cos-1 cells, and this limitation can be overcome by modification of the codon composition of the genes or by provision of excess tRNA. Replacement of codons in the green fluorescent protein (gfp) gene with those frequently used in PV late genes did not alter gfp transcription in Cos-1 cells but almost abolished translation, supporting the hypothesis that the observed differences in efficiency of translation of modified and unmodified PV capsid genes were related to codon usage rather than mRNA structure. As tRNA populations vary within and between tissues in the same eukaryotic organism, we speculate that matching of tRNA availability to codon usage may be one determinant of the restriction of expression of PV late genes to differentiated epithelium.  (+info)

Nucleotides 1506-1625 of bovine papillomavirus type 1 genome can enhance DNA packaging by L1/L2 capsids. (6/561)

We have previously described a DNA-packaging assay using bovine papillomavirus type 1 (BPV-1) virus-like particles (VLPs) and have identified a region of the BPV genome that assists in packaging. In this study, we identify a specific BPV sequence involved in DNA packaging by BPV-1 VLPs. In the initial screening of BPV-1 genomic sequences essential for DNA packaging, we observed that a plasmid with deletions between nucleotides (nt) 948 and 2113 failed to be packaged into BPV-1 VLPs. However, plasmids containing nt 948 to 2113 were efficiently packaged, suggesting that this 1.2-kb fragment contains a packaging enhancement sequence (PES). Further mapping of the BPV-1 genome showed that this packaging sequence lies between nt 1506 and 1625. Furthermore, this packaging sequence is also recognized by HPV6b VLPs, suggesting that a common packaging mechanism may be used by the two papillomavirus types. Given the phylogenetic difference between these two viral types, it is likely that other papillomavirus types may also use the same packaging mechanism. Identification of the PES has allowed a minimal viral genome sequence to be used in the packaging assay, improving the usefulness of the assay in studying the process of papillomavirus DNA encapsidation.  (+info)

Papillomavirus E2 induces p53-independent apoptosis in HeLa cells. (7/561)

We have previously shown that expression of the papillomavirus E2 protein in HeLa cells induces p53 accumulation and causes both cell cycle arrest and apoptosis. In contrast to growth arrest, onset of apoptosis was not correlated with an increase of p53 transcriptional activity. In the present study, we conducted biochemical and genetic experiments in order to determine whether E2-induced apoptosis was independent of p53 induction. We showed that E2 did not alter the transcription of Bax, a known p53-activated cell death inducer. The time course of apoptotic cell death preceded p53 induction by several hours. Overexpression of the HPV18 E6 oncogene prevented E2-mediated p53 accumulation, but did not alter the rate of cell death. Finally, point mutants of the HPV18 E2 transactivation domain induced apoptosis, although they were unable to induce high p53 accumulation or cell cycle arrest. In addition, the results obtained with these mutants indicated that both transcriptional activation and replication functions of E2 were dispensable for the induction of cell death. These observations show that E2-induced apoptosis is an early event, independent of p53 accumulation and unrelated to downstream p53-dependent transcriptional events.  (+info)

An enhanced epithelial response of a papillomavirus promoter to transcriptional activators. (8/561)

Mucosal epitheliotropic papillomaviruses have a similar long control region (LCR) organization: a promoter region, an enhancer region, and a highly conserved distribution of E2 DNA binding sites. The enhancer of these viruses is epithelial-specific, as it fails to activate transcription from heterologous promoters in nonepithelial cell types (Gloss, B., Bernard, H. U., Seedorf, K., and Klock, G. (1987) EMBO J. 6, 3735-3743; Morgan, I. M., Grindlay, G. J., and Campo, M. S. (1999) J. Gen. Virol. 80, 23-27). Studies on E2 transcriptional regulation of the human mucosal epitheliotropic papillomaviruses have been hindered by poor access to the natural target cell type and by the observation that some of the human papillomavirus promoters, including human papillomavirus-16, are repressed in immortalized epithelial cells. Here we present results using the bovine papillomavirus-4 (BPV-4) LCR and a bovine primary cell system as a model to study the mechanism of E2 transcriptional regulation of mucosal epitheliotropic papillomaviruses and the cell type specificity of this regulation. E2 up-regulates transcription from the BPV-4 LCR preferentially in epithelial cells (Morgan, I. M., Grindlay, G. J., and Campo, M. S. (1998) J. Gen. Virol. 79, 501-508). We demonstrate that the epithelial-specific enhancer element of the BPV-4 LCR is not required for the enhanced activity of E2 in epithelial cells and that the BPV-4 promoter is more responsive, not only to E2, but to other transcriptional activators in epithelial cells. This is the first time a level of epithelial specificity has been shown to reside in a papillomavirus promoter region.  (+info)

Bovine Papilloma Virus Type 1 E2 Monoclonal Antibody from Invitrogen for Immunofluorescence, Immunocytochemistry and ELISA applications. This antibody reacts with Virus samples. Clone: 3E8. Supplied as 100 ug purified antibody (1 mg/ml) in PBS with 50% glycerol and no preservative.
Mouse monoclonal antibody raised against Bovine papillomavirus type 1 E2. Recombinant protein corresponding to full length Bovine Papillomavirus type-1 transactivator protein E2. (MAB7861) - Products - Abnova
Samples of neoplastic and normal urothelium were obtained from cows originating from areas of southern Italy, a region in which chronic enzootic haematuria is endemic and bracken fern infestation is widespread. Specimens were analysed for bovine papillomavirus type 2 (BPV-2) DNA, BPV-2 E5 expression and telomerase activity. A total of 46 of 60 tumours and 17 of 34 normal bladder mucosa samples harboured BPV-2 DNA. Analysis of a subset of samples showed E5 protein expression and telomerase activity in tumour tissue only. No normal samples positive for BPV DNA showed E5 protein expression or telomerase activity, suggesting the presence of DNA in a latent state. Taken together, these data on naturally occurring bovine bladder tumours corroborate the hypothesis of their virus origin.
Bovine papillomavirus type 1 oncogene protein E5: 44-amino acid protein; a homo-dimeric, transmembrane protein that transforms cells by activating the platelet-derived growth factor ss receptor in a ligand-independent fashion and induces cellular DNA synthesis
In human cancer cells, BAG3 protein is known to sustain cell survival. Here, for the first time, we demonstrated the expression of BAG3 protein in equine sarcoids in vivo as well as in an in vitro model of sarcoid-derived equine fibroblasts. Evidence of a possible involvement of BAG3 in equine sarcoid carcinogenesis was obtained by immunohistochemistry analysis of tumour samples. We found that the most of tumour samples stained positive for BAG3, even though to a different grade, while normal dermal fibroblasts from a healthy horse displayed very weak staining pattern for BAG3 expression. By siRNA technology, we demonstrated the role of BAG3 in counteracting basal as well as chemical-triggered pro-death signals. BAG3 down-modulation in EqSO4b, a sarcoid-derived fully transformed cell line harbouring bovine papilloma virus (BPV)-1 genome, promotes cell death and cell cycle arrest in G0/G1. In addition, we found that BAG3 silencing sensitized cells to phenethylisothiocyanate (PEITC), a promising ...
The replication initiator protein E1 binds to the origin of replication of bovine papillomavirus in several forms. E1 can bind to its recognition sequence as a monomer together with the viral transcription factor E2, or as a trimeric E1 complex. The trimerization of E1 is mediated by the sequence-specific binding of E1 to DNA, and results in an E1 complex that is linked topologically to the DNA because the three molecules of E1 form a ring-like structure that encircles the DNA. These results demonstrate that E1 utilizes unusual mechanisms for sequence-specific binding to DNA and for the generation of a structure that encircles the DNA. We believe that these forms of E1 bound to the origin of replication represent intermediates in a transition in the function of E1, from a sequence-specific origin of replication recognition protein to a form of E1 that is competent for the initiation of viral DNA replication.. ...
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In this report, we show that Cox-2 is essential for optimal humoral immune responses against HPV type 16. These new findings reveal that Cox-2 deficiency impairs neutralizing Ab production following HPV 16 VLP vaccination. These data have important implications regarding the use of NSAIDs and Cox-2-selective inhibitors during vaccination. Drugs that inhibit Cox-2 are widely used for relief of symptoms of pain and inflammation associated with vaccination and other inflammatory conditions. Our findings reveal that the combination of reduced class switch recombination, B cell differentiation to ASCs and Ig secretion are responsible for impaired Ab production by HPV 16 VLP-vaccinated Cox-2−/− mice. The defect in Ab production by Cox-2−/− mice resulted in a dramatic reduction in VLP-specific neutralizing Ab titers (Fig. 5⇑). We demonstrated that Cox-2 is required for B cells to optimally differentiate into HPV 16 IgG-secreting cells by using an Ag-specific ELISPOT assay that detects the ...
In vitro replication of papillomavirus DNA has been carried out with a combination of purified proteins and partially purified extracts made from human cells. DNA synthesis requires the viral E1 protein and the papillomavirus origin of replication. The E2 protein stimulates DNA synthesis in a binding site-independent manner. Papillomavirus DNA replication is also dependent on the cellular factors replication protein A, replication factor C, and proliferating-cell nuclear antigen as well as a phosphocellulose column fraction (IIA). Fraction IIA contains DNA polymerase alpha-primase and DNA polymerase delta. Both of these polymerases are essential for papillomavirus DNA replication in vitro. However, unlike the case with T-antigen-dependent replication from the simian virus 40 origin, purified DNA polymerase alpha-primase and delta cannot efficiently replace fraction IIA in the replication reaction. Hence, additional cellular factors seem to be required for papillomavirus DNA replication. ...
The epidemiology of cervical cancer indicates the presence of a sexually transmitted risk factor, attributable at least in part to infection with human papillomavirus (HPV) type 16 or 18. We performed a seroepidemiological study of HPV and cervical cancer in the counties of Västerbotten and Norrbotten in Northern Sweden, a low-risk area for cervical cancer. Sera from 94 cases of incident cervical cancer were matched against 188 age- and sex-matched controls derived from a population-based blood bank. IgG and IgA antibodies were measured against a panel of 12 antigens derived from HPV types 6, 11,1 6, or 18, as well as against Herpes simplex virus type 1 and 2, Chlamydia trachomatis, cytomegalovirus, Epstein-Barr virus, and bovine papillomavirus. Significantly increased relative risks (RRs) were found for IgG to HPV 16- or 18-derived antigens from the L1 (RR = 3.1), E2 (RRs = 2.8 and 9.2), and E7 (RRs = 3.8 and 2.7) open reading frames and for IgA to HPV 16-derived antigens from the E2 (RR = ...
Wolf, M, Garcea RL, Grigorieff N, Harrison SC. 2010. Subunit interactions in bovine papillomavirus. Proc Natl Acad Sci U S A. 107(14):6298-6303. Abstract ...
Bovine papilloma antigen detected by fluorescent antibody was correlated with the appearance of infectious virus and with epithelial hyperplasia and hyperkeratinization in the developing bovine cutaneous papilloma. Neither antigen nor infectious virus was found in the earliest stages of growth characterized by connective tissue proliferation and by little or no epithelial change. The cellular localization of papilloma virus antigen in nuclear material of the superficial epithelium was the same when first observed as it was in the well developed wart. Regression of the warts was accompanied by a decrease in specific antigen. Marked variations in susceptibility of individual calves to bovine papilloma virus were observed, as well as differences in the histologic development of warts produced by virus administered intradermally and by scarification.. ...
Seventy six formalin-fixed paraffin-embedded sarcoids from 62 Australian horses, collected over a ten year period, were examined for the presence of genomic sequences from bovine papillomavirus 1 and 2 (BPV1, BPV2) with the polymerase chain reaction (PCR). Sequences that could be amplified by primers specific for BPV1 and BPV2 were present in 56 of the 76 sarcoids (73%). A restriction site present in BPV1 and absent from BPV2 was detected in 28 of 34 amplified products that were treated with endonuclease ...
Koch, C; Ramsauer, A S; Drögemüller, M; Ackermann, M; Gerber, V; Tobler, K (2018). Genomic comparison of bovine papillomavirus 1 isolates from bovine, equine and asinine lesional tissue samples. Virus Research, 244:6-12.. Kiefer, C; Tobler, K; Ramsauer, A C; Biegel, U; Kuehn, N; Ruetten, M (2017). Feline sarcoid in a 1-year-old domestic short-haired cat caused by bovine papillomavirus type 14 in Switzerland. Schweizer Archiv für Tierheilkunde, 159(9):487-491.. Lange, C E; Tobler, K; Lehner, A; Grest, P; Welle, M M; Schwarzwald, C C; Favrot, C (2013). EcPV2 DNA in equine papillomas and in situ and invasive squamous cell carcinomas supports papillomavirus etiology. Veterinary Pathology, 50(4):686-692.. Lange, C E; Vetsch, E; Ackermann, M; Favrot, C; Tobler, K (2013). Four novel papillomavirus sequences support a broad diversity among equine papillomaviruses. Journal of General Virology, 94(Pt 6):1365-1372.. Lange, C E; Tobler, K; Lehner, A; Vetsch, E; Favrot, C (2012). A case of a canine ...
The role of enzymes involved in polycomb repression of gene transcription has been studied extensively in human cancer. Polycomb repressive complexes mediate oncogene-induced senescence, a principal innate cell-intrinsic tumor suppressor pathway that thwarts expansion of cells that have suffered oncogenic hits. Infections with human cancer viruses including human papillomaviruses (HPVs) and Epstein-Barr virus can trigger oncogene-induced senescence, and the viruses have evolved strategies to abrogate this response in order to establish an infection and reprogram their host cells to establish a long-term persistent infection. As a consequence of inhibiting polycomb repression and evading oncogene induced-senescence, HPV infected cells have an altered epigenetic program as evidenced by aberrant homeobox gene expression. Similar alterations are frequently observed in non-virus associated human cancers and may be harnessed for diagnosis and therapy.
Human Papilloma virus type 16 E6 antibody for WB. Anti-Human Papilloma virus type 16 E6 pAb (GTX132686) is tested in Human papillomavirus samples. 100% Ab-Assurance.
Introduction Bovine papillomavirus (BPV) is the etiological agent of bovine papillomatosis, infectious and neoplastic disease, characterized by the presence of multiple papillomas that can regress spontaneously or to persist and progress to malignancies when in association with environmental cofactors. Although recognized that the BPV can induce DNA damages, the viral role following cancer initiation remains unresolved. Based on this, we stablished cell lines derived from cutaneous papilloma, fibropapilloma and esophageal carcinoma to study the BPV action on epithelial-mesenchymal transition (EMT). Our results showed strong evidences that the virus action can contribute to EMT and, therefore, metastasis. Aim In this study, we analyzed the expression levels of the EMT markers (cytokeratin 10, STAT3 Y705, Oct-3/4 and vimentin) in paraffin-embed samples, using the same tissues that originated the cell lines previous studied, aiming to validate the results observed using cell lines. Material and Methods
The skin of animals of a laboratory strain of Mastomys natalensis carrying endogenous, latent papillomavirus genomes was irritated by scratching with glasspaper. Hyperproliferation of the epidermis and amplification of viral DNA followed this treatment, and in approximately 27% of the animals virus-producing papillomas were induced.
These benign but bothersome tumors are as individual as the array of potential treatments. Theyre the most common type of skin tumor in horses, and theyre usually not all that alarming-but although theyre categorized as benign, sarcoids have plenty of troublesome potential.. The thing about sarcoids is, theyre devilishly difficult to defeat. Although they seem to have a common cause-a virus called bovine papilloma, which causes warts in cows-they are masters of disguise, taking any number of different forms and casually defying any kind of easy cure. Not all sarcoids need curing, of course. When they appear in non-troublesome places on the horses skin, and just sit there doing nothing in particular, they are better left alone. But sarcoids that sprout in more troublesome locations-such as the eyelid, the ear, or any spot where tack or equipment might rub or chafe-can cause more than their fair share of grief and need to be dealt with.. Research has been done on sarcoid tumors in horses and ...
Full Text AI-92-08 PAPILLOMAVIRUS IN VITRO CELL CULTURE SYSTEMS NIH GUIDE, Volume 21, Number 36, October 9, 1992 RFA: AI-92-08 P.T. 34 Keywords: Viral Studies (Virology) Cell Lines Chemotherapeutic Agents National Institute of Allergy and Infectious Diseases Letter of Intent Receipt Date: November 6, 1992 Application Receipt Date: December 10, 1992 PURPOSE The Antiviral Research Branch of the Division of Microbiology and Infectious Diseases (DMID), National Institute of Allergy and Infectious Diseases (NIAID), invites Cooperative Agreement applications from organizational entities willing to participate, with the assistance of the NIAID, in furthering innovative in vitro approaches to the study of papillomavirus infections and their therapeutic control. The goals of this solicitation are to stimulate the use of in vitro papillomavirus replication systems for research on (1) the events of papillomavirus replication and pathogenesis and (2) the antiviral potential of experimental therapeutic ...
A total number of 159 equine cases in different regions of Egypt were surveyed. The survey consisted of 83 horses and 76 donkeys of different ages affected wit...
[196 Pages Report] Check for Discount on Global Papillomavirus Diagnostics Market Country Shares and Segment Forecasts 2017-2021: Emerging Opportunities and Growth Strategies for Suppliers report by Venture Planning Group. The report presents a detailed analysis of the Papillomavirus diagnostics...
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L hépatite B est provoquée par un virus qui s attaque au foie. Atunci îi putem mulțumi iubitului tău pentru asta. Cest linterrogatoire du patient qui permet dorienter le diagnostic.
A highly sensitive, automated, purely add-on, high-throughput pseudovirion-based neutralization assay (HT-PBNA) with excellent repeatability and run-to-run reproducibility was developed for human papillomavirus types (HPV) 16, 18, 31, 45, 52, 58 and bovine papillomavirus type 1. Preparation of 384 well assay plates with serially diluted sera and the actual cell-based assay are separated in time, therefore batches of up to one hundred assay plates can be processed sequentially. A mean coefficient of variation (CV) of 13% was obtained for anti-HPV 16 and HPV 18 titers for a standard serum tested in a total of 58 repeats on individual plates in seven independent runs. Natural antibody response was analyzed in 35 sera from patients with HPV 16 DNA positive cervical intraepithelial neoplasia grade 2+ lesions. The new HT-PBNA is based on Gaussia luciferase with increased sensitivity compared to the previously described manual PBNA (manPBNA) based on secreted alkaline phosphatase as reporter. Titers ...
The precise aetiology of enzootic haematuria in cattle remains unknown. The involvement of bracken fern (Pteridium aquilinium) appears certain because of the close association between bracken fern infested farms and enzootic haematuria. Several toxic principles have been identified but the main carcinogenic element remains to be conclusively demonstrated. More recently, bovine papilloma virus has been implicated in the aetiology of enzootic haematuria. Its possible interaction with bracken fern carcinogen is discussed.. ...
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Beginning in 2012, our understanding of human papillomavirus (HPV) subcellular trafficking has undergone a drastic paradigm shift. Work from multiple laboratories has revealed that HPV has evolved a unique means to deliver its viral genome (vDNA) to the cell nucleus, relying on a myriad of host cell proteins and processes. The major breakthrough finding from these recent endeavors was the realization of L2-dependent utilization of cellular sorting factors for the retrograde transport of vDNA away from degradative endo/lysosomal compartments to the Golgi, prior to mitosis-dependent nuclear accumulation of L2/vDNA. An overview of current models of HPV entry, subcellular trafficking, and the role of L2 during initial infection is provided below, highlighting unresolved questions and gaps in knowledge.
Hargrove, Stacy A.; Work, Thierry M.; Brunson, Shandell; Foley, Allen Michael; Balazs, George H.; Girard, Alexandre; Stacy, Brian A. (Brian Adams); Diez, Carlos; Baptistotte, Cecilia; Limpus, Colin James; Walsh, Daniel; Lynch, Jennifer; Jones, Karina; Ehrhart, Llewellyn; Chaloupka, Milani; Van Houtan, Kyle Schuyler, 1974-; Murakawa, Shawn K. K ...
Bakgrunn: Infeksjon med humant papillomavirus er den vanligste seksuelt overførbare infeksjonen både hos kvinner og menn. Det er anslått at opptil 20 % av den seksuelt aktive befolkningen på et hvert tidspunkt er smittet, og at over 70 % av befolkningen vil få en HPV- infeksjon i løpet av sitt seksuelt aktive liv. Høsten 2009 ble vaksinen mot humant papilloma virus (HPV) innført i barnevaksinasjonsprogrammet for jenter på 7. klassetrinn. Som for alle vaksiner i barnevaksinasjonsprogrammet er dette et tilbud man kan velge å ta i mot. Siden denne vaksinen er ny, vil det være usikkerhet knyttet til i hvor stor grad foreldrene vil tillate at vaksinen anvendes på døtrene. I denne forbindelse har jeg valgt å undersøke vurderinger foreldrene gjør for å bestemme seg om de skal la døtrene sine vaksineres eller ikke. Material og metode: Denne oppgaven er et litteraturstudium. I utarbeidelsen har jeg brukt søkeportalen PubMed. I samarbeid med veileder har jeg funnet de mest relevante ...
The aim of this study was to investigate microRNA (miRNA) differential expression in the two most common equine skin tumours, equine sarcoid (ES) and squamous cell carcinoma (SCC), and its potential influence on the tumour microenvironment at post‐transcriptional level. We investigated miRNA fingerprints in four subgroups: mild (ESM) and aggressive (ESA) ES and ocular SCC (oSCC) and genital SCC (g ...
mouse C9orf10 protein: An accessory role of C9orf10 for Puralpha in a limited brain region in addition to other possible functions that have not yet been determined.
We have sequenced 1730 bp of human papilloma virus type 18 (HPV 18) DNA containing the open reading frames (ORF) E6, E7, the N-terminal part of E1 and, additionally, 120 bp of the N-terminal part of L1. Based on these sequencing data, together with the human papilloma virus type 16 (HPV 16) DNA sequence published recently, we identified and cloned the ORF E6, E7, E1 and L1 of HPV 18 and the ORF E6, E7, E1, E4, E5, L2 and L1 of HPV 16 into prokaryotic expression vectors. The expression system used provides fusions to the N-terminal part of the MS2 polymerase gene controlled by the heat-inducible lambda PL promoter. Using the purified fusion proteins as immunogens we raised antisera against the proteins encoded by the ORF E6, E7 and E1 of HPV 18 as well as those encoded by the ORF E6, E7, E4 and L1 of HPV 16. By Western blot analysis we could show that the E7 gene product is the most abundant protein in cell lines containing HPV 16 or HPV 18 DNA. It is a cytoplasmic protein of 15 kd in the SiHa and the
High-risk human papillomavirus type 16 (HPV16) is a risk factor for cervical cancer. Previous studies suggest that polymorphisms in the E6 gene or the long control region(LCR)of HPV16 may alter the oncogenic potential of the virus. The aims of this study were to investigate the genetic variations of HPV16 E6 gene and LCR in isolates from Chinese population and correlation of the E6 and LCR polymorphisms with disease status of infected patients. HPV16 positive endocervical specimens were collected from 304 women living in Northeast of China. Sequences of E6 gene and LCR were analyzed by PCR-sequencing. Two lineages were found in the populations, including EUR lineage and As lineage. Based on the HPV16 prototype, the most frequent variation in the E6 gene was T178A/G (48.7%), followed by mutations of G94A (12.2%) and T350G (9.9%). The rank orders of incidence of E6 variations in amino acid were as follows: D25E (46.3%), L83V (9.9%) and H78Y (4.3%). Nucleotide variations in LCR were found in all the 304
MmuPV1, a papillomavirus that infects laboratory mice (Mus musculus), is discovered to be sexually transmitted, providing a new animal virus model to study sexually transmitted human papillomaviruses (HPVs).
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I take sarcoids very seriously for one reason alone; they are a type of skin tumour/cancer. I frequently come across clients who have been told in the past to either leave a sarcoid, or try a treatment often purchased off the internet. At this point, I usually ask the client how they would approach treatment of skin cancer if they had been diagnosed with the condition by their doctor… leave it to see how it grows, ignore it, go online and buy a cream which claims to cure a multitude of aliments, or go to their doctor and speak to an oncologist (a doctor that specialises in cancer therapy) and treat accordingly?. No matter how small a sarcoid is, once found contact your vet and treat it immediately. Trust me, it will not only save your horse but save you time, money and heartache in the long run. It is my personal opinion that a sarcoid should never be ignored or left.. Continued below…. ...
The expression of the Papillomavirus genome is complex because there are multiple promoters, alternative splicing patterns, and a link between differentiation and gene expression. Only one strand of the genome is transcribed, producing two classes of proteins: the non-structural regulatory proteins, known as early proteins, and the structural proteins L1 and L2, known as late proteins. The transformation process is very complex and depends largely on the early gene products. The transferring proteins appear to vary from one virus type to another and the function and mechanism of these transforming proteins are still not clear. The general principle appears to be that two or more early proteins co-operate to give a transforming phenotype.The most confusing thing is that in most cases, all or part of the Papilloma genome including the putative "transforming genes" is maintained in the tumour cells, whereas in other cases, the virus DNA may be lost after transformation. BPV-4 is an example of one ...
マウス・モノクローナル抗体 ab2417 適用: WB,ELISA,IHC-P…HPV抗体一覧…画像、プロトコール、文献などWeb上の情報が満載のアブカムの Antibody 製品。国内在庫と品質保証制度も充実。
Human papillomavirus type 11 L1 protein, Human papillomavirus type 16 L1 protein, Human papillomavirus type 18 L1 protein, Human papillomavirus type 31 L1 protein, Human papillomavirus type 33 L1 protein, Human papillomavirus type 45 L1 protein, Human papillomavirus type 52 L1 protein, Human papillomavirus type 6 L1 protein, Human papillomavirus type 58 L1 ...
The activity and epithelial tropism of the human papillomavirus type 18 P105 early promoter, which directs the synthesis of the E6 and E7 transforming genes, are controlled by cis elements included in the viral long control region. To identify potential cellular regulators of this promoter, we mutagenized one or both of the 5-TGACTAA-3 cis elements capable of interacting with the AP1 transcription factor, which is composed either of homodimers or heterodimers of the Jun products or of heterodimers of Jun and Fos. Mutation of both elements completely abolished P105 promoter activity in human keratinocytes. We show that either AP1 site can interact efficiently in vitro with any of the three different Jun products as heterodimers with c-Fos. However, in nuclear extracts prepared from human keratinocytes, JunB was the predominant Jun component bound to the DNA probe containing this cis element. These results implicate JunB as an important factor in human papillomavirus type 18 transcription in ...
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p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
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Prevalentemente attraverso i rapporti sessuali. Forma infiltrativa propagarea cancerului se face endocevical Tratament 2 Stadii preinvazive a.
Pour le Grand Public  La Fin d'un Cancer ?Qu'est-ce qu'un papillomavirus ou HPV ?Tous les droits d'auteur seront reversés à l'association 1000 Femmes...
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MKlp2 is a kinesin-like motor protein of the central mitotic spindle required for completion of cytokinesis. Papillomavirus E2 is a sequence specific DNA binding protein that regulates viral transcription and replication and is responsible for partitioning viral episomes into daughter cells during cell division. We demonstrate that MKlp2 specifically associates with the E2 protein during mitosis. Using chromatin immunoprecipitation, we show viral genomes are in complex with MKlp2 only within this stage of cell cycle. By immunofluorescence, a subpopulation of papillomavirus E2 colocalizes with MKlp2 in the midbody/midplate during late mitosis. We conclude that during specific stages of mitosis, the papillomavirus E2 protein binds to MKlp2, and infer that association with this motor protein ensures viral genome partitioning during cytokinesis.
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Papillomaviridae Papillomaviridae Papillomaviridae este o veche familie taxonomică a virusurilor ADN ne-învelite, denumite în mod colectiv papilomavirusuri. Apasă pentru a vedea traducerea automată a definiției în Română. Cnd sunt zgriate ele pot sngera.
The Papillomavirus HPV is only one of pretty much the most intensive explicitly shipped illnesses venereal afflictions in the United States and probably on the planet furthermore. Previous investigation study studies demonstrate that the gathering of people affected by this disease in the USA independent from anyone else may be between 15 to 20 zillion, which permit by a type of requests. Two or three other appearance into demonstrate the basic truth that more than 80 % of females will without a doubt contract this issue a long time before they accomplish 50 years obsolete. This virus is add-on among the critical take a gander at the progression of cervical malignant growths cell material, an infirmity that is typically destructive. There exists more than 100 tension and stress and nervousness in the person Papillomavirus HPV, a large number of them low-carcinogenic. Related with 30 of these is acquired with sexual reach. They have really been seen that any kind of kind of adult containing as a ...
Recombinant HPV-11 antigen is a 58.1kDa protein covering the full length of HPV-11 major capsid, its N-terminus is fused with a GST tag, having a total molecular weight of 84kDa. The HPV-11 was purified by proprietary chromatographic technique. ...
Schiller, J. T.; Vass, W. C.; Vousden, K. H.; Lowy, D. R. (1986). "E5 open reading frame of bovine papillomavirus type 1 ... Lechner, M. S.; Mack, D. H.; Finicle, A. B.; Crook, T.; Vousden, K. H.; Laimins, L. A. (1992). "Human papillomavirus E6 ... Vousden's early work focused on the molecular biology of human papillomaviruses (HPVs), which are associated with cervical ... she led the Human Papillomavirus Group at the Ludwig Institute for Cancer Research, London, UK. In 1995, she joined the ...
Crystal structure at 1.7 Å of the bovine papillomavirus-1 E2 DMA-binding domain bound to its DNA target. Nature 359, 505-512 ( ... His interests are cervical cancer, papillomaviruses and epithellia cells. His highest cited paper is Human papillomavirus ... Pathogenesis of Human Papillomaviruses in Differentiating Epithelia. Microbiol. Mol. Biol. Rev. June 2004 vol. 68 no. 2 362-372 ... Biosynthesis of human papillomavirus from a continuous cell line upon epithelial differentiation. Science Vol. 257, Iss. 5072 ...
Vande Pol SB, Brown MC, Turner CE (Jan 1998). "Association of Bovine Papillomavirus Type 1 E6 oncoprotein with the focal ... 293 (1): 38-52. doi:10.1016/j.ydbio.2005.12.040. PMID 16533505. Gehmlich K, Pinotsis N, Hayess K, van der Ven PF, Milting H, El ... 513 (1): 114-8. doi:10.1016/s0014-5793(01)03244-6. PMID 11911889. Salgia R, Li JL, Lo SH, Brunkhorst B, Kansas GS, Sobhany ES, ... 4 (1): 146-52. doi:10.4161/cam.4.1.10973. PMC 2852571 . PMID 20139696. Check date values in: ,date= (help) Wood CK, Turner CE, ...
Several studies have found an association between the presence of Bovine papillomavirus-1 and 2 and associated viral growth ... "Association of bovine papillomavirus with the equine sarcoid". Journal of General Virology. 84 (5): 1055-1062. doi:10.1099/vir. ... "Intralesional bovine papillomavirus DNA loads reflect severity of equine sarcoid disease". Equine Veterinary Journal. 42 (4): ... Equine papillomavirus-2 has also been found within penile SCCs, but has not been determined to cause SCC. Before treatment of ...
2001), "Roles of the hinge region and the DNA binding domain of the bovine papillomavirus type 1 E2 protein in initiation of ... 201-228, ISBN 1-4020-5561-7 Hemphill, B.E.; Lukacs, J.R.; Kennedy, K.A.R. (1991). "Biological Adaptations and Affinities of ... 15 (1): 121-6. doi:10.1038/sj.ejhg.5201726. PMC 2588664 . PMID 17047675. Sengupta et al. (2005) Underhill, Peter A; et al. ( ... 54 (1): 47-55. doi:10.1038/jhg.2008.2. PMID 19158816. Mirabal, Sheyla; et al. (2009). "Y-Chromosome distribution within the geo ...
Similar papillomaviruses of ungulates (e.g. deer papillomavirus, European elk papillomavirus, ovine papillomavirus 1,2) are ... "Bovine papillomavirus: old system, new lessons?". In Campo, MS. Papillomavirus Research: From Natural History to Vaccine and ... Bovine papillomavirus (BPV) is a group of DNA viruses of the family Papillomaviridae that are common in cattle. Infection ... Chambers G, Ellsmore VA, O'Brien PM, Reid SW, Love S, Campo MS, Nasir L (2003). "Association of bovine papillomavirus with the ...
... papillomavirus MeSH B04.909.204.210.655.600.650 --- papillomavirus, bovine MeSH B04.909.204.210.655.600.660 --- papillomavirus ... papillomavirus MeSH B04.909.574.204.655.600.650 --- papillomavirus, bovine MeSH B04.909.574.204.655.600.660 --- papillomavirus ... papillomavirus MeSH B04.280.535.600.650 --- papillomavirus, bovine MeSH B04.280.535.600.660 --- papillomavirus, cottontail ... papillomavirus MeSH B04.909.624.600.650 --- papillomavirus, bovine MeSH B04.909.624.600.660 --- papillomavirus, cottontail ...
Fibropapillomas are present in other animal groups, but are caused by different viruses, for example the bovine papillomavirus ... 2012). "A Histopathological, Immunohistochemical and Molecular Study of Cutaneous Bovine Papillomatosis" (PDF). Kafkas Univ Vet ... 41 (1): 29-41. doi:10.7589/0090-3558-41.1.29. Quackenbush, S. L.; Casey, R. N.; Murcek, R. J.; Paul, T. A.; Work, T. M.; et al ... 7 (1): 114-125. doi:10.1016/j.hal.2007.06.001. Tan, M. T.; Yildirim, Y.; Sozmen, M.; Bilge-Dagalp, S.; Yilmaz, V.; et al. ( ...
You J, Croyle JL, Nishimura A, Ozato K, Howley PM (Apr 2004). "Interaction of the bovine papillomavirus E2 protein with Brd4 ... Abbate EA, Voitenleitner C, Botchan MR (Dec 2006). "Structure of the papillomavirus DNA-tethering complex E2:Brd4 and a peptide ... Baxter MK, McPhillips MG, Ozato K, McBride AA (Apr 2005). "The mitotic chromosome binding activity of the papillomavirus E2 ... Schweiger MR, You J, Howley PM (May 2006). "Bromodomain protein 4 mediates the papillomavirus E2 transcriptional activation ...
"Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication". Journal of ... Kaldalu N; Lepik D; Kristjuhan A; Ustav M. (2000). "Monitoring and purification of proteins using bovine papillomavirus E2 ... Icosagen's main products are: FITkit (latex allergen test) and FITkit testing service [1] E2Tag epitope tagging technology MAbs ...
Pseudocowpox Warts caused by bovine papillomavirus Teat-end hyperkeratosis Dermatitis Frostbite Udder sores or necrotic ... ISBN 978-0-13-046256-5. Ruegg, Pamela L. "Diseases of Bovine Teats and Skin - Reproductive System". Merck Veterinary Manual. ... Rowen D. Frandson; W. Lee Wilke; Anna Dee Fails (1 April 2013), Anatomy and Physiology of Farm Animals, John Wiley & Sons, pp. ... 449-451, ISBN 978-1-118-68601-0 "Mammalian Milk". www.earthlife.net. Retrieved 12 August 2017. "Teat Structure Chart". American ...
The E5 protein of some animal papillomavirus types (mainly bovine papillomavirus type 1) functions as an oncogene primarily by ... Only a few papillomavirus types encode a short protein from the E8 gene. In the case of BPV-4 (papillomavirus genus Xi), the E8 ... Jackson ME, Pennie WD, McCaffery RE, Smith KT, Grindlay GJ, Campo MS (1991). "The B subgroup bovine papillomaviruses lack an ... Coggins LW, Ma JQ, Slater AA, Campo MS (1985). "Sequence homologies between bovine papillomavirus genomes mapped by a novel low ...
The best studied example is the association between Human papillomavirus and cervical cancer: almost all cases of cervical ... Creutzfeldt-Jakob disease and bovine spongiform encephalopathy ("mad cow disease"), are caused by prions, hepatitis D is due to ... Harald zur Hausen had shown that two strains of Human papillomavirus (HPV) cause most cases of cervical cancer. Two vaccines ... 100 (1): 119-46. PMID 17592822. Van Epps HL (2005). "Peyton Rous: Father of the tumor virus" (PDF). Journal of Experimental ...
See also: Papillomavirus and Bovine papillomavirus. This section is empty. You can help by adding to it. (December 2017) ... Warts are caused by the human papilloma virus (HPV). There are about 130 known types of human papilloma viruses.[8] HPV infects ... a b Human Papillomavirus Archived 2015-08-23 at the Wayback Machine.. Public Health Agency of Canada ... Warts are caused by infection with a type of human papillomavirus (HPV).[1] Factors that increase the risk include use of ...
The human papillomavirus vaccine is recommended in the U.S. (as of 2011) and UK (as of 2009). Vaccine recommendations for the ... Bovine herpesvirus 1 DIVA vaccines are also widely used in practice. Scientists have put and still, are putting much effort in ... In the case of a few relatively new vaccines such as the human papillomavirus vaccine, the patents may impose an additional ... Chang Y, Brewer NT, Rinas AC, Schmitt K, Smith JS (July 2009). "Evaluating the impact of human papillomavirus vaccines". ...
Such benefits include male insects transferring nutrients to females via their ejaculate; in both humans and bovines the fluid ... This includes transmission risk for sexually transmitted diseases such as human papillomavirus (HPV) or herpes, especially for ... Retrieved 1 December 2016. Rafik, Amal. Passion Masters: Sex Secrets of a Forbidden Cult. Google Books: Blue Moon Books. ISBN ... ISBN 1-4179-1276-6. Retrieved November 9, 2013. Meyer, Marvin (2007). "The Testimony of Truth". The Nag Hammadi Scriptures. ...
Bat mastadenovirus A Bat mastadenovirus B Bovine mastadenovirus A Bovine mastadenovirus B Bovine mastadenovirus C Canine ... de Villiers EM, Fauquet C, Broker TR, Bernard HU, zur Hausen H. Classification of papillomaviruses. Virology. 2004;324(1):17-27 ... Bovine rhinitis A virus Bovine rhinitis B virus Equine rhinitis A virus Foot-and-mouth disease virus Genus: Aquamavirus ... Bovine leukemia virus Primate T-lymphotropic virus 1 Primate T-lymphotropic virus 2 Primate T-lymphotropic virus 3 Genus: ...
The human papillomavirus vaccine is recommended in the U.S. (as of 2011)[63] and UK (as of 2009).[64] Vaccine recommendations ... Bovine herpesvirus 1 DIVA vaccines are also widely used in practice. Other DIVA vaccines (under development). Scientists have ... "HPV Vaccine , Human Papillomavirus , CDC". www.cdc.gov. 2019-05-13. Retrieved 2019-06-14.. ... In the case of a few relatively new vaccines such as the human papillomavirus vaccine, the patents may impose an additional ...
"Human papillomavirus infection in head and neck cancer: the role of the secretory leukocyte protease inhibitor". Oncology ... crystal structure of the acid-stable proteinase inhibitor from human mucous secretions analysed in its complex with bovine ... it has been shown that there is an inverse correlation between the levels of SLPI and high-risk Human Papillomavirus (HPV) ... "The role of the antileukoprotease SLPI in smoking-induced human papillomavirus-independent head and neck squamous cell ...
One area of particular interest is the study of human papilloma viruses (HPV) and their role in cervical cancers. Researchers ... Having finally lost completely its virulence, the bovine tuberculosis germ grown with their method was the principal ... he focused on creating a vaccine using the bacillus responsible for bovine tuberculosis, very similar to the human one, as it ... In addition to the isolation of HIV-1 and HIV-2, in the recent past researchers at the Institut Pasteur have developed a test ...
If biotin was used as a probe label, non-specific binding sites must first be blocked using bovine serum albumin (BSA). Then, ... Apart from cancers, CISH has also been shown to be useful in detecting human papillomavirus infections. SISH uses a similar ... doi:10.1007/978-1-60761-789-1_1. ISBN 978-1-60761-788-4. PMID 20809300. Lambros, M. B.; Simpson, P. T.; Jones, C; Natrajan, R; ... 50 (1): 26-30. doi:10.2302/kjm.50.26. PMID 11296661. Darouich, S; Popovici, C; Missirian, C; Moncla, A (2012). "Use of DOP-PCR ...
... is an infection by human papillomavirus (HPV).[4] Most HPV infections cause no symptoms and ... An HPV infection is caused by human papillomavirus, a DNA virus from the papillomavirus family, of which over 170 types are ... "Pink Book (Human Papillomavirus)" (PDF). CDC.gov. Archived (PDF) from the original on 21 March 2017. Retrieved 18 April 2017.. ... "Human Papillomavirus". Medscape. Archived from the original on 29 November 2016.. *^ "Photos of larynx Papillomas - Voice ...
a b Human Papillomavirus at eMedicine *^ Egawa K, Kitasato H, Honda Y, Kawai S, Mizushima Y, Ono T (1998). "Human ... They are caused by the human papillomavirus (HPV).[2] A break in the skin is required for infection to occur.[2] Risk factors ... "Human Papillomaviruses Compendium" (PDF). Los Alamos National Laboratory. Retrieved 2013-02-05.. ... Plantar warts are benign epithelial tumors generally caused by infection by human papillomavirus types 1, 2, 4, 60, or 63,[5] ...
Retrieved 1 March 2016. Saier, M. H.; Yen, M. R.; Noto, K.; Tamang, D. G.; Elkan, C. (1 January 2009). "The Transporter ... Retrieved 1 March 2016. [1] [2] [3] [4] Transporter Classification Database List at qmul.ac.uk Saier MH, Tran CV, Barabote RD ( ... 3.D.1 The H+ or Na+-translocating NADH Dehydrogenase ("complex I") family 3.D.2 The Proton-translocating Transhydrogenase (PTH ... Retrieved 1 March 2016. Saier, M. H.; Tran, C. V.; Barabote, R. D. (1 January 2006). "TCDB: the Transporter Classification ...
The polyoma and papillomaviruses appear to have evolved from single-stranded DNA viruses and ultimately from plasmids. The ... includes the species bovine stool associated circular virus and chimpanzee stool associated circular virus. The closest ... 158 (1): 283-9. doi:10.1007/s00705-012-1470-0. PMID 22972681. Kim AR, Chung HC, Kim HK, Kim EO, Nguyen VG, Choi MG, Yang HJ, ... 1 (4): e00558-13. doi:10.1128/genomeA.00558-13. PMC 3738887 . PMID 23929471. Cheung, A. K.; Ng, T. F.-f.; Lager, K. M.; Alt, D ...
One area of particular interest is the study of human papilloma viruses (HPV) and their role in cervical cancers. Researchers ... Having finally lost completely its virulence, the bovine tuberculosis germ grown with their method was the principal ... he focused on creating a vaccine using the bacillus responsible for bovine tuberculosis, very similar to the human one, as it ... In addition to the isolation of HIV-1 and HIV-2, in the recent past researchers at the Institut Pasteur have developed a test ...
To investigate the efficiency of encapsidation of plasmid by papillomavirus virus-like particles (PV VLPs), and the infectivity ... of the resultant PV pseudovirions, Cos-1 cells were transfected with... ... Efficiency of delivery of DNA to cells by bovine papillomavirus type-1 L1/L2 pseudovirions. ... To investigate the efficiency of encapsidation of plasmid by papillomavirus virus-like particles (PV VLPs), and the infectivity ...
Bovine Papilloma Virus Type 1 E2 Monoclonal Antibody from Invitrogen for Immunofluorescence, Immunocytochemistry and ELISA ... Supplied as 100 ug purified antibody (1 mg/ml) in PBS with 50% glycerol and no preservative. ... Cite Bovine Papilloma Virus Type 1 E2 Monoclonal Antibody (3E8). The following antibody was used in this experiment: Bovine ... View (7) other Bovine Papilloma Virus Type 1 E2 antibodies Invitrogen Bovine Papilloma Virus Type 1 E2 Monoclonal Antibody (3E8 ...
Anogenital type human papillomavirus (HPV) E6 oncoproteins and E6 from bovine papillomavirus type 1 (BE6) interact with ... Papillomavirus E6 proteins are adapters that change the function of cellular regulatory proteins. The bovine papillomavirus ... Transformation by Bovine Papillomavirus Type 1 E6 Requires Paxillin. Ramon Wade, Nicole Brimer, Scott Vande Pol ... Transformation by Bovine Papillomavirus Type 1 E6 Requires Paxillin Message Subject (Your Name) has forwarded a page to you ...
Bovine papillomavirus type 1 oncogene protein E5: 44-amino acid protein; a homo-dimeric, transmembrane protein that transforms ... Bovine papillomavirus type 1 oncogene protein E5. Subscribe to New Research on Bovine papillomavirus type 1 oncogene protein E5 ... oncogene protein E5, Bovine papillomavirus type 1; E5 protein, Bovine papilloma virus type 1; E5 transforming protein, BPV-1; v ...
Recombinant protein corresponding to full length Bovine Papillomavirus type-1 transactivator protein E2. (MAB7861) - Products ... Mouse monoclonal antibody raised against Bovine papillomavirus type 1 E2. ... Bovine Papillomavirus type 1 E2-protein. Mapped to amino acids 199-208 (SSTSSDFRDR). ... Recombinant protein corresponding to full length Bovine Papillomavirus type-1 transactivator protein E2. ...
Transcriptional and replicational activation functions in the bovine papillomavirus type 1 E2 protein are encoded by different ... Transcriptional and replicational activation functions in the bovine papillomavirus type 1 E2 protein are encoded by different ... Transcriptional and replicational activation functions in the bovine papillomavirus type 1 E2 protein are encoded by different ... Transcriptional and replicational activation functions in the bovine papillomavirus type 1 E2 protein are encoded by different ...
Campo MS: Bovine papillomavirus: Old system, new lessons?. Papillomavirus Research: From Natural History to Vaccines and Beyond ... Bovine papillomavirus type 1 infects not only cattle but also equids and is a causative factor in the pathogenesis of commonly ... Bogaert L, Van Poucke M, De Baere C, Dewulf J, Peelman L, Ducatelle R, Gasthuys F, Martens A: Bovine papillomavirus load and ... Equine sarcoids: Bovine Papillomavirus type 1 transformed fibroblasts are sensitive to cisplatin and UVB induced apoptosis and ...
Bovine papillomavirus type 1 (BPV-1) activity is necessary for the transformation phenotype of equine fibroblasts. Among the ... Animals, Binding Sites, Bovine papillomavirus 1, Cell Line, Gene Expression Profiling, Horses, Host-Pathogen Interactions, ... Finally, BPV-1 E5, E6 and E7 proteins increased MMP-1 mRNA and protein expression. In conclusion, the expression of MMP-1 can ... c-Jun and Fra-1, two components of the AP-1 transcription factor complex, were overexpressed and activated by BPV-1 in equine ...
Similar papillomaviruses of ungulates (e.g. deer papillomavirus, European elk papillomavirus, ovine papillomavirus 1,2) are ... "Bovine papillomavirus: old system, new lessons?". In Campo, MS. Papillomavirus Research: From Natural History to Vaccine and ... Bovine papillomavirus (BPV) is a group of DNA viruses of the family Papillomaviridae that are common in cattle. Infection ... Chambers G, Ellsmore VA, OBrien PM, Reid SW, Love S, Campo MS, Nasir L (2003). "Association of bovine papillomavirus with the ...
Bovine papillomavirus type 1 (BPV-1) and less commonly BPV-2 are now widely recognized as the causative agents of the disease. ... Knockdown of BPV-1 oncogene expression by small interfering RNA reversed the transformed phenotype of sarcoid fibroblasts. ... However, it remains unclear to what extent BPV-1 proteins are involved in the transformation of equine cells. To address this ... Together, these observations strongly suggest that BPV-1 proteins play indispensable roles in the transformation of equine ...
bovine papillomavirus type 1 (synthetic). Aethiops sabeus (African Green Monkey). No. SIE. HTML , XML. ... human papillomavirus (HPV-16). Homo sapiens. Yes. UE. HTML , XML. 147. E2 (16 E2-dm). Wang 2003. human papillomavirus (HPV-16) ... human papillomavirus (HPV-16). Homo sapiens. No. SIE. HTML , XML. 149. L2h. Leder 2001. human papillomavirus (HPV-16). Homo ... Human papillomavirus (HPV). Solanum tuberosum. No. UE. HTML , XML. 268. L1s. Warzecha 2003. Human papillomavirus (HPV). Solanum ...
Bovine immunodeficiency virus. Homo sapiens. No. SIE. HTML , XML. 40. L1 (HB PV L1). Gu 2004. Bovine papillomavirus type 1. ... human papillomavirus (HPV-16). Homo sapiens. Yes. SIE. HTML , XML. 187. E7. Kim MS 2005. human papillomavirus (HPV-16). Mus ... human papillomavirus (HPV-16). Homo sapiens. Yes. IE. HTML , XML. 182. E1 (16 E1-dm). Wang 2003. human papillomavirus (HPV-16) ... Human papillomavirus. Homo sapiens. Yes. SIE. HTML , XML. 174. E7h. Cheung 2004. Human papillomavirus. Mus musculus. Unknown. ...
BAG3 down-modulation in EqSO4b, a sarcoid-derived fully transformed cell line harbouring bovine papilloma virus (BPV)-1 genome ... BAG3 down-modulation in EqSO4b, a sarcoid-derived fully transformed cell line harbouring bovine papilloma virus (BPV)-1 genome ... BAG3 protects Bovine Papillomavirus type 1-transformed equine fibroblasts against pro-death signals. ... BAG3 protects Bovine Papillomavirus type 1-transformed equine fibroblasts against pro-death signals ...
Intranuclear inclusions in renal tubular epithelium in immunodeficient mice stain with antibodies for bovine papillomavirus ... Immunohistochemistry using polyclonal anti-bovine papillomavirus type 1 (BPV-1) L1 antibody, Camvir monoclonal anti- ... Intranuclear inclusions in renal tubular epithelium in immunodeficient mice stain with antibodies for bovine papillomavirus ... papillomavirus antibody (directed against the seven amino acids GFGAMDF found in human papillomavirus (HPV) 16 L1 protein), a ...
Fingerprint Dive into the research topics of Interaction with CBP/p300 enables the bovine papillomavirus type 1 E6 oncoprotein ... The E6 oncoprotein of bovine papillomavirus type 1 (BPV-1) can transform cells independently of p53 degradation. The precise ... N2 - The E6 oncoprotein of bovine papillomavirus type 1 (BPV-1) can transform cells independently of p53 degradation. The ... AB - The E6 oncoprotein of bovine papillomavirus type 1 (BPV-1) can transform cells independently of p53 degradation. The ...
bovine papillomavirus type 1 (synthetic). Aethiops sabeus (African Green Monkey). No. SIE. HTML , XML. ... Human papillomavirus. Mus musculus. Unknown. NR. HTML , XML. 30. L1h. Cheung 2004. Human papillomavirus. Homo sapiens. Yes. SIE ... human papillomavirus (HPV-16). Homo sapiens. Yes. UE. HTML , XML. 234. E2 (16 E2-dm). Wang 2003. human papillomavirus (HPV-16) ... human papillomavirus (HPV-16). Homo sapiens. No. SIE. HTML , XML. 127. L1h. Leder 2001. human papillomavirus (HPV-16). Homo ...
bovine papillomavirus type 1 (synthetic). Aethiops sabeus (African Green Monkey). No. SIE. HTML , XML. ... Canine oral papillomavirus. Canis familiaris. Unknown. IE. HTML , XML. 37. E7. Kim MS 2005. human papillomavirus (HPV-16). Mus ... Human papillomavirus (HPV). Nicotiana tabacum. No. EE. HTML , XML. 133. L1h. Leder 2001. human papillomavirus (HPV-16). Homo ... Human papillomavirus (HPV). Solanum tuberosum. No. UE. HTML , XML. 135. L1p. Leder 2001. human papillomavirus (HPV-16). Homo ...
From Human papillomavirus type 16.. E6. P06931. 3. EBI-8516738,EBI-7281937. From Bovine papillomavirus type 1. ... "Interaction of oncogenic papillomavirus E6 proteins with fibulin-1.". Du M., Fan X., Hong E., Chen J.J.. Biochem. Biophys. Res ... Microbial infection) Interacts with human papillomavirus/HPV type 16, 18 and 31 proteins E6.1 Publication. ,p>Manually curated ... Cited for: INTERACTION WITH HIGH-RISK HUMAN PAPILLOMAVIRUSES E6 PROTEIN (MICROBIAL INFECTION), INHIBITION OF E6-MEDIATED ...
The three-dimensional structure of bovine papillomavirus has been determined to 9 A resolution by reconstruction of high ... Novel structural features of bovine papillomavirus capsid revealed by a three-dimensional reconstruction to 9 A resolution Nat ... The three-dimensional structure of bovine papillomavirus has been determined to 9 A resolution by reconstruction of high ... B L Trus 1 , R B Roden, H L Greenstone, M Vrhel, J T Schiller, F P Booy ...
... was developed to investigate the effect of bovine papillomavirus E1 protein on host cell growth. Expression of E1 in CE1 cells ... Bovine papillomavirus 1 / genetics, metabolism*. Cattle. Cell Cycle / physiology*. Cell Division. Cell Line, Transformed. DNA- ... A stable E1-expressing cell line, CE1, was developed to investigate the effect of bovine papillomavirus E1 protein on host cell ... 0/DNA-Binding Proteins; 0/E1 protein, Bovine papillomavirus; 0/Recombinant Fusion Proteins; 0/Viral Proteins; 50-02-2/ ...
Differential expression of microRNAs in bovine papillomavirus type 1 transformed equine cells. ... The impact of tick-borne pathogen infection in Indian bovines is determined by host type but not the genotype of Theileria ... Characterization of HSP90 isoforms in transformed bovine leukocytes infected with Theileria annulata. ... 2018 Nov 1;144:274-284. doi: 10.1016/j.watres.2018.07.039. Epub 2018 Jul 17. ...
Nuclear import of bovine papillomavirus type 1 E1 protein is mediated by multiple alpha importins and is negatively regulated ... Identification of a nuclear export signal sequence for bovine papillomavirus E1 protein. ... Proteins of the PIAS family enhance the sumoylation of the papillomavirus E1 protein. ... 2011 Jun;158(1-2):12-27. doi: 10.1016/j.virusres.2011.02.017. Epub 2011 Mar 3. ...
Bovine papillomavirus (BPV) is perhaps the most extensively studied animal papillomavirus. In cattle BPVs induce benign tumours ... Bovine papillomaviruses: their role in the aetiology of cutaneous tumours of bovids and equids.. Nasir L1, Campo MS. ... The purpose of this review is to discuss the biology of BPV, the biology of bovine tumours and equine sarcoids, and present the ... Only limited information on the clinical or pathological aspects of either bovine or equine tumours will be provided as this ...
... Lies ... "A Novel Murine Model for Evaluating Bovine Papillomavirus Prophylactics/therapeutics for Equine Sarcoid-like Tumours." Journal ... "A Novel Murine Model for Evaluating Bovine Papillomavirus Prophylactics/therapeutics for Equine Sarcoid-like Tumours." JOURNAL ... Equine sarcoids are highly recurrent bovine papillomavirus (BPV)-induced fibroblastic neoplasms that are the most common skin ...
bovine papillomavirus type 1 (synthetic). Aethiops sabeus (African Green Monkey). No. SIE. HTML , XML. ... human papillomavirus type 16 (HPV16). Homo sapiens. Yes. SIE. HTML , XML. 145. hu-mE7. Zhao 2002. Human papillomavirus (HPV). ... human papillomavirus (HPV-16). Homo sapiens. Yes. UE. HTML , XML. 204. E2 (16 E2-dm). Wang 2003. human papillomavirus (HPV-16) ... human papillomavirus (HPV-16). Homo sapiens. No. SIE. HTML , XML. 94. L1h. Leder 2001. human papillomavirus (HPV-16). Homo ...
  • Presence of bovine papillomavirus type 2 DNA and expression of the viral oncoprotein E5 in naturally occurring urinary bladder tumours in cows. (microbiologyresearch.org)
  • Many studies have suggested a possible link between breast cancer pathogenesis and viral infection, particularly mouse mammary tumour virus, simian virus 40, Epstein-Barr virus, and human papillomavirus (HPV). (springer.com)
  • 4. The immunostimulatory regimen of claim 1 wherein the an agonistic CD40L polypeptide or CD40 agonistic antibody or antibody fragment, type 1 interferon and viral antigen are administered as discrete polypeptides. (google.com.au)
  • 8. The immunostimulatory regimen of claim 2 wherein the an agonistic CD40L polypeptide or CD40 agonistic antibody or antibody fragment, type 1 interferon and viral antigen are administered as discrete compounds in combination. (google.com.au)
  • Publication date: Available online 25 May 2019Source: Antiviral ResearchAuthor(s): B. Praena, R. Bello-Morales, F. de Castro, J.A. López-GuerreroAbstractHerpes simplex virus type 1 (HSV-1) is a ubiquitous infectious agent that can establish latency in neurons, and in some cases, viral retrograde transport results in infection of the central nervous system (CNS). (medworm.com)
  • The papillomavirus E2 proteins bind to Brd4, and some utilize this interaction in tethering the viral genomes to mitotic chromosomes ( 1 , 3 , 57 , 59 ). (asm.org)
  • Herpes simplex virus type 1 ICP0 plays a critical role in the de novo synthesis of infectious virus following transfection of viral DNA. (asm.org)
  • In HIV-1 infection, unspliced and singly spliced viral mRNA is not transported into the cytoplasm unless the posttranscriptional regulator Rev is present ( 21 , 23 , 38 ). (asm.org)
  • We have examined the roles of these four E2 sites in the viral life cycle of HPV type 31 (HPV31) by using recently developed methods for the biosynthesis of papillomaviruses from transfected DNA templates (M. G. Frattini et al. (asm.org)
  • We have demonstrated an important role of pX ORF I expression, which encodes p12 I , in establishment of HTLV-1 infection in a rabbit model and for optimal viral infectivity in quiescent primary lymphocytes. (asm.org)
  • Additionally, we also demonstrated that ORF I expression is required for optimal viral infectivity in quiescent primary lymphocytes, suggesting a role of p12 I in T-lymphocyte activation ( 1 ). (asm.org)
  • Several studies have found an association between the presence of Bovine papillomavirus-1 and 2 and associated viral growth proteins in skin cells with sarcoid formation, but the exact mechanism that controls or induces epidermal proliferation remains unknown. (wikipedia.org)
  • The human immunodeficiency virus type 1 (HIV-1) glycoprotein gp160 is processed into the transmembrane subunit (TM) gp41 and the nonconvalently linked gp120 glycoprotein, which binds to the CD4 receptor and chemokine coreceptor molecules. (asm.org)
  • In total, HIV-1 expresses 16 canonical proteins from only nine genes within its 10 kb genome. (frontiersin.org)
  • Arranged in three different reading frames, it contains only nine canonical genes (Figure 1 ). (frontiersin.org)
  • O segundo par de primers utilizado é complementar a sequência dos genes E5 e L2 de BPVs 1, 2 e 13. (scielo.br)
  • Papillomaviruses (PVs) are highly epitheliotropic as they usually establish productive infections within squamous epithelia of the skin, the anogenital tract and the oral cavity. (nih.gov)
  • This study shows that both active and productive infections by BPV-2 in the urothelium of the bovine and bubaline urinary bladder can occur in vivo. (nih.gov)
  • As observed by Robbins et al 1 and Plotkin, 2 almost all current vaccines work by the induction of antibodies in serum or on the mucosa (by local production or transudation from serum) to block adherence of pathogens to epithelial cells or interfere with microbial invasion of the bloodstream. (aappublications.org)
  • Detection of antibodies against epidermodysplasia verruciformis-associated canine Papillomavirus 3 in sera of dogs from Europe and Africa by enzyme-linked immunosorbent assay. (uzh.ch)
  • Characterization of a herpes simplex virus type 2 75,000-molecular-weight glycoprotein antigenically related to herpes simplex virus type 1 glycoprotein C. (asm.org)
  • Like equine sarcoids, these murine tumour cells (BPV-KB) were of fibroblast origin, were tumorigenic and expressed BPV-1 proteins. (ugent.be)
  • As an initial investigation of the preclinical potential of this tumour model for equine sarcoids prophylactics, mice were immunized with BPV-1 E5E6 Venezuelan equine encephalitis virus replicon particles, prior to BPV-KB challenge, which resulted in an increased tumour-free period compared with controls, indicating that the BPV-KB murine model may be a valuable preclinical alternative to equine clinical trials. (ugent.be)
  • The bovine papillomavirus (BPV) types 1 and 2 may be involved in the pathogenesis of sarcoids, and probably the recently described BPV type (BPV13) might be associated with the pathogenesis of this lesion. (scielo.br)
  • Overlapping peptides comprising amino acids (aa) 2 to 22 (hereafter, chimera or peptide 2-22), 13 to 107, 18 to 31, 17 to 36, 35 to 75, 75 to 112, 115 to 154, 149 to 175, and 172 to 200 of HPV type 16 (HPV16) L2 were genetically engineered into the DE surface loop of bovine papillomavirus type 1 L1 VLP. (nih.gov)
  • BPV-4 causes squamous cell carcinomas of the alimentary tract, and BPV-1/2 causes carcinomas and haemangioendotheliomas of the urinary bladder, in both cases in animals that have fed on bracken (Pteridium aquilinum). (wikipedia.org)
  • EcPV2 DNA in equine papillomas and in situ and invasive squamous cell carcinomas supports papillomavirus etiology. (uzh.ch)