A species of DELTAPAPILLOMAVIRUS infecting cattle.
A type of XIPAPILLOMAVIRUS causing alimentary carcinoma in cattle. It is related to Bovine papillomavirus 3.
Neoplasms of the skin and mucous membranes caused by papillomaviruses. They are usually benign but some have a high risk for malignant progression.
A family of small, non-enveloped DNA viruses infecting birds and most mammals, especially humans. They are grouped into multiple genera, but the viruses are highly host-species specific and tissue-restricted. They are commonly divided into hundreds of papillomavirus "types", each with specific gene function and gene control regions, despite sequence homology. Human papillomaviruses are found in the genera ALPHAPAPILLOMAVIRUS; BETAPAPILLOMAVIRUS; GAMMAPAPILLOMAVIRUS; and MUPAPILLOMAVIRUS.
Products of viral oncogenes, most commonly retroviral oncogenes. They usually have transforming and often protein kinase activities.
A type of ALPHAPAPILLOMAVIRUS especially associated with malignant tumors of the CERVIX and the RESPIRATORY MUCOSA.
ONCOGENE PROTEINS from papillomavirus that deregulate the CELL CYCLE of infected cells and lead to NEOPLASTIC CELL TRANSFORMATION. Papillomavirus E7 proteins have been shown to interact with various regulators of the cell cycle including RETINOBLASTOMA PROTEIN and certain cyclin-dependent kinase inhibitors.
Vaccines or candidate vaccines used to prevent PAPILLOMAVIRUS INFECTIONS. Human vaccines are intended to reduce the incidence of UTERINE CERVICAL NEOPLASMS, so they are sometimes considered a type of CANCER VACCINES. They are often composed of CAPSID PROTEINS, especially L1 protein, from various types of ALPHAPAPILLOMAVIRUS.
Deoxyribonucleic acid that makes up the genetic material of viruses.
A type of human papillomavirus especially associated with malignant tumors of the genital and RESPIRATORY MUCOSA.
Infections produced by oncogenic viruses. The infections caused by DNA viruses are less numerous but more diverse than those caused by the RNA oncogenic viruses.
A type of ALPHAPAPILLOMAVIRUS causing recurrent respiratory PAPILLOMATOSIS; GENITAL WARTS; and other neoplasms.
Tumors or cancer of the UTERINE CERVIX.
Proteins found in any species of virus.
An inheritable change in cells manifested by changes in cell division and growth and alterations in cell surface properties. It is induced by infection with a transforming virus.
A circumscribed benign epithelial tumor projecting from the surrounding surface; more precisely, a benign epithelial neoplasm consisting of villous or arborescent outgrowths of fibrovascular stroma covered by neoplastic cells. (Stedman, 25th ed)
The type species of KAPPAPAPILLOMAVIRUS. It is reported to occur naturally in cottontail rabbits in North America.
A genus of DNA viruses in the family PAPILLOMAVIRIDAE causing fibropapillomas in their respective ungulate hosts. Species infected include cattle, European elk, deer, and sheep.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
Benign epidermal proliferations or tumors; some are viral in origin.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
The functional hereditary units of VIRUSES.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A genus of DNA viruses in the family PAPILLOMAVIRIDAE. They preferentially infect the anogenital and ORAL MUCOSA in humans and primates, causing both malignant and benign neoplasms. Cutaneous lesions are also seen.
A type of ALPHAPAPILLOMAVIRUS usually associated with GENITAL WARTS; and LARYNGEAL NEOPLASMS.
Any of the processes by which cytoplasmic factors influence the differential control of gene action in viruses.
Established cell cultures that have the potential to propagate indefinitely.
The process by which a DNA molecule is duplicated.
Diseases of domestic cattle of the genus Bos. It includes diseases of cows, yaks, and zebus.
A malignancy arising in uterine cervical epithelium and confined thereto, representing a continuum of histological changes ranging from well-differentiated CIN 1 (formerly, mild dysplasia) to severe dysplasia/carcinoma in situ, CIN 3. The lesion arises at the squamocolumnar cell junction at the transformation zone of the endocervical canal, with a variable tendency to develop invasive epidermoid carcinoma, a tendency that is enhanced by concomitant human papillomaviral infection. (Segen, Dictionary of Modern Medicine, 1992)
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Diseases of domestic and wild horses of the species Equus caballus.
Proteins that form the CAPSID of VIRUSES.
Sexually transmitted form of anogenital warty growth caused by the human papillomaviruses.
A type of ALPHAPAPILLOMAVIRUS associated with high risk for anogenital neoplasms.
The neck portion of the UTERUS between the lower isthmus and the VAGINA forming the cervical canal.
Eukaryotic cell line obtained in a quiescent or stationary phase which undergoes conversion to a state of unregulated growth in culture, resembling an in vitro tumor. It occurs spontaneously or through interaction with viruses, oncogenes, radiation, or drugs/chemicals.
The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.
A PDGF receptor that binds specifically to the PDGF-B chain. It contains a protein-tyrosine kinase activity that is involved in SIGNAL TRANSDUCTION.
Collection of pooled secretions of the posterior vaginal fornix for cytologic examination.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A unique DNA sequence of a replicon at which DNA REPLICATION is initiated and proceeds bidirectionally or unidirectionally. It contains the sites where the first separation of the complementary strands occurs, a primer RNA is synthesized, and the switch from primer RNA to DNA synthesis takes place. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Cis-acting DNA sequences which can increase transcription of genes. Enhancers can usually function in either orientation and at various distances from a promoter.
Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Cell changes manifested by escape from control mechanisms, increased growth potential, alterations in the cell surface, karyotypic abnormalities, morphological and biochemical deviations from the norm, and other attributes conferring the ability to invade, metastasize, and kill.
Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.
DNA probes specific for the identification of human papilloma virus.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
The complete genetic complement contained in a DNA or RNA molecule in a virus.
The outer protein protective shell of a virus, which protects the viral nucleic acid.
Pathological processes of the UTERINE CERVIX.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
Proteins transcribed from the E2 region of ADENOVIRUSES. Several of these are required for viral DNA replication.
Ruminants of the family Bovidae consisting of Bubalus arnee and Syncerus caffer. This concept is differentiated from BISON, which refers to Bison bison and Bison bonasus.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Large, hoofed mammals of the family EQUIDAE. Horses are active day and night with most of the day spent seeking and consuming food. Feeding peaks occur in the early morning and late afternoon, and there are several daily periods of rest.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Specific receptors on cell membranes that react with PLATELET-DERIVED GROWTH FACTOR, its analogs, or antagonists. The alpha PDGF receptor (RECEPTOR, PLATELET-DERIVED GROWTH FACTOR ALPHA) and the beta PDGF receptor (RECEPTOR, PLATELET-DERIVED GROWTH FACTOR BETA) are the two principle types of PDGF receptors. Activation of the protein-tyrosine kinase activity of the receptors occurs by ligand-induced dimerization or heterodimerization of PDGF receptor types.
A family of hoofed MAMMALS consisting of HORSES, donkeys, and zebras. Members of this family are strict herbivores and can be classified as either browsers or grazers depending on how they feed.
A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Immunoglobulins produced in response to VIRAL ANTIGENS.
The infective system of a virus, composed of the viral genome, a protein core, and a protein coat called a capsid, which may be naked or enclosed in a lipoprotein envelope called the peplos.
Methods for detecting or typing the DNA of an ALPHAPAPILLOMAVIRUS in biological tissues and fluids.
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
Ribonucleic acid that makes up the genetic material of viruses.
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
Abnormal development of immature squamous EPITHELIAL CELLS of the UTERINE CERVIX, a term used to describe premalignant cytological changes in the cervical EPITHELIUM. These atypical cells do not penetrate the epithelial BASEMENT MEMBRANE.
Cancers or tumors of the PENIS or of its component tissues.
Cytological preparation of cells collected from a mucosal surface and stained with Papanicolaou stain.
Tumors or cancer of the VULVA.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
Processes that stimulate the GENETIC TRANSCRIPTION of a gene or set of genes.
Serum albumin from cows, commonly used in in vitro biological studies. (From Stedman, 25th ed)
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
Suspensions of attenuated or killed viruses administered for the prevention or treatment of infectious viral disease.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
A species of VARICELLOVIRUS that causes INFECTIOUS BOVINE RHINOTRACHEITIS and other associated syndromes in CATTLE.
A carcinoma derived from stratified SQUAMOUS EPITHELIAL CELLS. It may also occur in sites where glandular or columnar epithelium is normally present. (From Stedman, 25th ed)
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
An idiopathic systemic inflammatory granulomatous disorder comprised of epithelioid and multinucleated giant cells with little necrosis. It usually invades the lungs with fibrosis and may also involve lymph nodes, skin, liver, spleen, eyes, phalangeal bones, and parotid glands.
Genes whose gain-of-function alterations lead to NEOPLASTIC CELL TRANSFORMATION. They include, for example, genes for activators or stimulators of CELL PROLIFERATION such as growth factors, growth factor receptors, protein kinases, signal transducers, nuclear phosphoproteins, and transcription factors. A prefix of "v-" before oncogene symbols indicates oncogenes captured and transmitted by RETROVIRUSES; the prefix "c-" before the gene symbol of an oncogene indicates it is the cellular homolog (PROTO-ONCOGENES) of a v-oncogene.
Tumors or cancer of the OROPHARYNX.
Tumors or cancer of the ANAL CANAL.
The examination, therapy or surgery of the cervix and vagina by means of a specially designed endoscope introduced vaginally.
Nucleic acid sequences involved in regulating the expression of genes.
An autosomal recessive trait with impaired cell-mediated immunity. About 15 human papillomaviruses are implicated in associated infection, four of which lead to skin neoplasms. The disease begins in childhood with red papules and later spreads over the body as gray or yellow scales.
Genes which regulate or circumscribe the activity of other genes; specifically, genes which code for PROTEINS or RNAs which have GENE EXPRESSION REGULATION functions.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
A genus of DNA viruses in the family PAPILLOMAVIRIDAE, which cause cutaneous lesions in humans. They are histologically distinguishable by intracytoplasmic INCLUSION BODIES which are species specific.
A genus of DNA viruses in the family PAPILLOMAVIRIDAE, causing cutaneous lesions in humans. Infections exist in latent form in the general population and are activated under conditions of IMMUNOSUPPRESSION.
The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).
CELL LINES derived from the CV-1 cell line by transformation with a replication origin defective mutant of SV40 VIRUS, which codes for wild type large T antigen (ANTIGENS, POLYOMAVIRUS TRANSFORMING). They are used for transfection and cloning. (The CV-1 cell line was derived from the kidney of an adult male African green monkey (CERCOPITHECUS AETHIOPS).)
Process of generating a genetic MUTATION. It may occur spontaneously or be induced by MUTAGENS.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.
Tumors or cancer of the SKIN.
A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
Tumors or cancer of the VAGINA.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
Biochemical identification of mutational changes in a nucleotide sequence.
Tumors or cancer of the PALATINE TONSIL.
CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.
Cell lines whose original growing procedure consisted being transferred (T) every 3 days and plated at 300,000 cells per plate (J Cell Biol 17:299-313, 1963). Lines have been developed using several different strains of mice. Tissues are usually fibroblasts derived from mouse embryos but other types and sources have been developed as well. The 3T3 lines are valuable in vitro host systems for oncogenic virus transformation studies, since 3T3 cells possess a high sensitivity to CONTACT INHIBITION.
Proteins that catalyze the unwinding of duplex DNA during replication by binding cooperatively to single-stranded regions of DNA or to short regions of duplex DNA that are undergoing transient opening. In addition DNA helicases are DNA-dependent ATPases that harness the free energy of ATP hydrolysis to translocate DNA strands.
A species of POLYOMAVIRUS originally isolated from Rhesus monkey kidney tissue. It produces malignancy in human and newborn hamster kidney cell cultures.
Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.
The type species of DELTARETROVIRUS that causes a form of bovine lymphosarcoma (ENZOOTIC BOVINE LEUKOSIS) or persistent lymphocytosis.
Viral proteins that are components of the mature assembled VIRUS PARTICLES. They may include nucleocapsid core proteins (gag proteins), enzymes packaged within the virus particle (pol proteins), and membrane components (env proteins). These do not include the proteins encoded in the VIRAL GENOME that are produced in infected cells but which are not packaged in the mature virus particle,i.e. the so called non-structural proteins (VIRAL NONSTRUCTURAL PROTEINS).
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Sites on an antigen that interact with specific antibodies.

Induction of autoantibodies to mouse CCR5 with recombinant papillomavirus particles. (1/561)

The vertebrate immune system has evolved to respond vigorously to microbial infection but to ignore self-antigens. Evidence has emerged that B cell responses to viruses are initiated by immune recognition of ordered arrays of antigen on the viral surface. To test whether autoantibodies against a self-antigen can be induced by placing it in a context that mimics the ordered surface of a viral particle, a peptide representing an extracellular loop of the mouse chemokine receptor CCR5 was incorporated into an immunodominant site of the bovine papillomavirus virus L1 coat protein, which self-assembles into virus-like particles. Mice inoculated with chimeric L1-CCR5 particles generated autoantibodies that bound to native mouse CCR5, inhibited binding of its ligand RANTES, and blocked HIV-1 infection of an indicator cell line expressing a human-mouse CCR5 chimera. These results suggest a general method for inducing autoantibodies against self-antigens, with diverse potential basic research and clinical applications.  (+info)

A mutational analysis of the transforming functions of the E8 protein of bovine papillomavirus type 4. (2/561)

The E8 protein of BPV-4 contributes to transformation of primary bovine cells (PalFs) by inducing anchorage-independent growth and by down-regulating gap junction intercellular communication, likely due to its binding to 16K ductin. We show here that, in addition, E8 confers on PalF cells the ability to grow in low serum and to escape from contact inhibition (focus formation). E8 also transactivates an exogenous human cyclin A gene promoter, suggesting that overexpression of cyclin A is responsible for the transformed phenotype. Mutant forms of E8 were generated to establish whether the transforming functions of the protein could be segregated. Mutations were introduced both in the hydrophobic domain and in the hydrophilic C-terminal "tail", and chimeras with BPV-1 E5 were constructed. Cells expressing either wild-type E8 or mutant forms were analyzed for their ability to grow in low serum and in suspension and to form foci. Wild-type E8 and its mutants were also analyzed for their ability to transactivate the cyclin A promoter. We show here that the transforming functions of E8 can be segregated and that both the hydrophilic C-terminal tail and the residue at position 17 in the hydrophobic domain are crucial for E8 functions and for the transactivation of the cyclin A promoter. These results support the hypothesis that the different aspects of cellular transformation brought about by E8 might be due to interaction with different cellular targets. They suggest that E8 might function differently from BPV-1 E5 and demonstrate that the separate domains of E5 and E8 are not functionally interchangeable.  (+info)

Long-term episomal maintenance of bovine papillomavirus type 1 plasmids is determined by attachment to host chromosomes, which Is mediated by the viral E2 protein and its binding sites. (3/561)

Papillomavirus genomes are stably maintained as extrachromosomal nuclear plasmids in dividing host cells. To address the mechanisms responsible for stable maintenance of virus, we examined nuclear compartmentalization of plasmids containing the full-length upstream regulatory region (URR) from the bovine papillomavirus type 1 (BPV1) genome. We found that these plasmids are tightly associated with the nuclear chromatin both in the stable cell lines that maintain episomal copies of the plasmids and in transiently transfected cells expressing the viral E1 and E2 proteins. Further analysis of viral factors revealed that the E2 protein in trans and its multiple binding sites in cis are both necessary and sufficient for the chromatin attachment of the plasmids. On the other hand, the BPV1 URR-dependent plasmid replication and chromatin attachment processes are clearly independent of each other. The ability of the plasmids to stably maintain episomes correlates clearly with their chromatin association function. These data suggest that viral E2 protein-mediated attachment of BPV1 genomes to the host cell chromatin could provide a mechanism for the coupling of viral genome multiplication and partitioning to the host cell cycle during viral latent infection.  (+info)

Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication. (4/561)

The bovine papillomavirus type 1 (BPV-1) E2 protein is the master regulator of papillomavirus replication and transcription. We have raised a panel of monoclonal antibodies (MAbs) against the BPV-1 E2 protein and used them to probe the structure and function of the protein. Five MAbs reacted with linear epitopes, and four MAbs recognized conformation-dependent epitopes which mapped within the C-terminal DNA-binding and dimerization domain. MAb 1E2 was able to recognize the replication- and transactivation-defective but not the competent conformation of the transactivation domain of the E2 protein. MAb 5H4 prevented efficiently the formation of E2-DNA as well as E2-dependent E1-E2-origin complexes and also dissociated preformed complexes in a concentration-dependent manner. Cotransfection of several MAbs with the BPV-1 minimal origin plasmid pUCAlu into CHO4.15 cells resulted in a dose-dependent inhibition of replication. Inhibition of replication by MAb 5H4 and the Fab' fragment of 5H4 correlated with their ability to dissociate the E2 protein from the DNA. MAb 3F12 and MAbs 1H10 and 1E4, directed against the hinge region, were also capable of inhibiting BPV-1 origin replication in CHO4.15 cells. However, the Fab' fragments of 1H10 and 3F12 had no effect in the transient replication assay. These data suggest that MAbs directed against the hinge region sterically hinder the inter- or intramolecular interactions required for the replication activity of the E2 protein.  (+info)

Papillomavirus capsid protein expression level depends on the match between codon usage and tRNA availability. (5/561)

Translation of mRNA encoding the L1 and L2 capsid proteins of papillomavirus (PV) is restricted in vivo to differentiated epithelial cells, although transcription of the L1 and L2 late genes occurs more widely. The codon composition of PV late genes is quite different from that of most mammalian genes. To test the possibility that PV late gene codon composition determines the efficiency of PV late gene expression in some cell types, synthetic bovine papillomavirus type 1 (BPV1) late genes were constructed with codon composition modified to resemble the typical mammalian gene. Expression of these genes from a strong promoter in Cos-1 cells was compared with expression of wild-type BPV1 late genes from the same promoter. Both unmodified and modified PV late genes were transcribed in Cos-1 cells, but only the codon-modified genes were translated. In vitro translation of wild-type but not synthetic BPV1 L1 mRNA was markedly enhanced by addition of aminoacyl-tRNAs. Codon composition thus limits BPV1 late gene translation in Cos-1 cells, and this limitation can be overcome by modification of the codon composition of the genes or by provision of excess tRNA. Replacement of codons in the green fluorescent protein (gfp) gene with those frequently used in PV late genes did not alter gfp transcription in Cos-1 cells but almost abolished translation, supporting the hypothesis that the observed differences in efficiency of translation of modified and unmodified PV capsid genes were related to codon usage rather than mRNA structure. As tRNA populations vary within and between tissues in the same eukaryotic organism, we speculate that matching of tRNA availability to codon usage may be one determinant of the restriction of expression of PV late genes to differentiated epithelium.  (+info)

Nucleotides 1506-1625 of bovine papillomavirus type 1 genome can enhance DNA packaging by L1/L2 capsids. (6/561)

We have previously described a DNA-packaging assay using bovine papillomavirus type 1 (BPV-1) virus-like particles (VLPs) and have identified a region of the BPV genome that assists in packaging. In this study, we identify a specific BPV sequence involved in DNA packaging by BPV-1 VLPs. In the initial screening of BPV-1 genomic sequences essential for DNA packaging, we observed that a plasmid with deletions between nucleotides (nt) 948 and 2113 failed to be packaged into BPV-1 VLPs. However, plasmids containing nt 948 to 2113 were efficiently packaged, suggesting that this 1.2-kb fragment contains a packaging enhancement sequence (PES). Further mapping of the BPV-1 genome showed that this packaging sequence lies between nt 1506 and 1625. Furthermore, this packaging sequence is also recognized by HPV6b VLPs, suggesting that a common packaging mechanism may be used by the two papillomavirus types. Given the phylogenetic difference between these two viral types, it is likely that other papillomavirus types may also use the same packaging mechanism. Identification of the PES has allowed a minimal viral genome sequence to be used in the packaging assay, improving the usefulness of the assay in studying the process of papillomavirus DNA encapsidation.  (+info)

Papillomavirus E2 induces p53-independent apoptosis in HeLa cells. (7/561)

We have previously shown that expression of the papillomavirus E2 protein in HeLa cells induces p53 accumulation and causes both cell cycle arrest and apoptosis. In contrast to growth arrest, onset of apoptosis was not correlated with an increase of p53 transcriptional activity. In the present study, we conducted biochemical and genetic experiments in order to determine whether E2-induced apoptosis was independent of p53 induction. We showed that E2 did not alter the transcription of Bax, a known p53-activated cell death inducer. The time course of apoptotic cell death preceded p53 induction by several hours. Overexpression of the HPV18 E6 oncogene prevented E2-mediated p53 accumulation, but did not alter the rate of cell death. Finally, point mutants of the HPV18 E2 transactivation domain induced apoptosis, although they were unable to induce high p53 accumulation or cell cycle arrest. In addition, the results obtained with these mutants indicated that both transcriptional activation and replication functions of E2 were dispensable for the induction of cell death. These observations show that E2-induced apoptosis is an early event, independent of p53 accumulation and unrelated to downstream p53-dependent transcriptional events.  (+info)

An enhanced epithelial response of a papillomavirus promoter to transcriptional activators. (8/561)

Mucosal epitheliotropic papillomaviruses have a similar long control region (LCR) organization: a promoter region, an enhancer region, and a highly conserved distribution of E2 DNA binding sites. The enhancer of these viruses is epithelial-specific, as it fails to activate transcription from heterologous promoters in nonepithelial cell types (Gloss, B., Bernard, H. U., Seedorf, K., and Klock, G. (1987) EMBO J. 6, 3735-3743; Morgan, I. M., Grindlay, G. J., and Campo, M. S. (1999) J. Gen. Virol. 80, 23-27). Studies on E2 transcriptional regulation of the human mucosal epitheliotropic papillomaviruses have been hindered by poor access to the natural target cell type and by the observation that some of the human papillomavirus promoters, including human papillomavirus-16, are repressed in immortalized epithelial cells. Here we present results using the bovine papillomavirus-4 (BPV-4) LCR and a bovine primary cell system as a model to study the mechanism of E2 transcriptional regulation of mucosal epitheliotropic papillomaviruses and the cell type specificity of this regulation. E2 up-regulates transcription from the BPV-4 LCR preferentially in epithelial cells (Morgan, I. M., Grindlay, G. J., and Campo, M. S. (1998) J. Gen. Virol. 79, 501-508). We demonstrate that the epithelial-specific enhancer element of the BPV-4 LCR is not required for the enhanced activity of E2 in epithelial cells and that the BPV-4 promoter is more responsive, not only to E2, but to other transcriptional activators in epithelial cells. This is the first time a level of epithelial specificity has been shown to reside in a papillomavirus promoter region.  (+info)

Bovine Papilloma Virus Type 1 E2 Monoclonal Antibody from Invitrogen for Immunofluorescence, Immunocytochemistry and ELISA applications. This antibody reacts with Virus samples. Clone: 3E8. Supplied as 100 ug purified antibody (1 mg/ml) in PBS with 50% glycerol and no preservative.
Mouse monoclonal antibody raised against Bovine papillomavirus type 1 E2. Recombinant protein corresponding to full length Bovine Papillomavirus type-1 transactivator protein E2. (MAB7861) - Products - Abnova
Samples of neoplastic and normal urothelium were obtained from cows originating from areas of southern Italy, a region in which chronic enzootic haematuria is endemic and bracken fern infestation is widespread. Specimens were analysed for bovine papillomavirus type 2 (BPV-2) DNA, BPV-2 E5 expression and telomerase activity. A total of 46 of 60 tumours and 17 of 34 normal bladder mucosa samples harboured BPV-2 DNA. Analysis of a subset of samples showed E5 protein expression and telomerase activity in tumour tissue only. No normal samples positive for BPV DNA showed E5 protein expression or telomerase activity, suggesting the presence of DNA in a latent state. Taken together, these data on naturally occurring bovine bladder tumours corroborate the hypothesis of their virus origin.
Bovine papillomavirus type 1 oncogene protein E5: 44-amino acid protein; a homo-dimeric, transmembrane protein that transforms cells by activating the platelet-derived growth factor ss receptor in a ligand-independent fashion and induces cellular DNA synthesis
In human cancer cells, BAG3 protein is known to sustain cell survival. Here, for the first time, we demonstrated the expression of BAG3 protein in equine sarcoids in vivo as well as in an in vitro model of sarcoid-derived equine fibroblasts. Evidence of a possible involvement of BAG3 in equine sarcoid carcinogenesis was obtained by immunohistochemistry analysis of tumour samples. We found that the most of tumour samples stained positive for BAG3, even though to a different grade, while normal dermal fibroblasts from a healthy horse displayed very weak staining pattern for BAG3 expression. By siRNA technology, we demonstrated the role of BAG3 in counteracting basal as well as chemical-triggered pro-death signals. BAG3 down-modulation in EqSO4b, a sarcoid-derived fully transformed cell line harbouring bovine papilloma virus (BPV)-1 genome, promotes cell death and cell cycle arrest in G0/G1. In addition, we found that BAG3 silencing sensitized cells to phenethylisothiocyanate (PEITC), a promising ...
The replication initiator protein E1 binds to the origin of replication of bovine papillomavirus in several forms. E1 can bind to its recognition sequence as a monomer together with the viral transcription factor E2, or as a trimeric E1 complex. The trimerization of E1 is mediated by the sequence-specific binding of E1 to DNA, and results in an E1 complex that is linked topologically to the DNA because the three molecules of E1 form a ring-like structure that encircles the DNA. These results demonstrate that E1 utilizes unusual mechanisms for sequence-specific binding to DNA and for the generation of a structure that encircles the DNA. We believe that these forms of E1 bound to the origin of replication represent intermediates in a transition in the function of E1, from a sequence-specific origin of replication recognition protein to a form of E1 that is competent for the initiation of viral DNA replication.. ...
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In this report, we show that Cox-2 is essential for optimal humoral immune responses against HPV type 16. These new findings reveal that Cox-2 deficiency impairs neutralizing Ab production following HPV 16 VLP vaccination. These data have important implications regarding the use of NSAIDs and Cox-2-selective inhibitors during vaccination. Drugs that inhibit Cox-2 are widely used for relief of symptoms of pain and inflammation associated with vaccination and other inflammatory conditions. Our findings reveal that the combination of reduced class switch recombination, B cell differentiation to ASCs and Ig secretion are responsible for impaired Ab production by HPV 16 VLP-vaccinated Cox-2−/− mice. The defect in Ab production by Cox-2−/− mice resulted in a dramatic reduction in VLP-specific neutralizing Ab titers (Fig. 5⇑). We demonstrated that Cox-2 is required for B cells to optimally differentiate into HPV 16 IgG-secreting cells by using an Ag-specific ELISPOT assay that detects the ...
In vitro replication of papillomavirus DNA has been carried out with a combination of purified proteins and partially purified extracts made from human cells. DNA synthesis requires the viral E1 protein and the papillomavirus origin of replication. The E2 protein stimulates DNA synthesis in a binding site-independent manner. Papillomavirus DNA replication is also dependent on the cellular factors replication protein A, replication factor C, and proliferating-cell nuclear antigen as well as a phosphocellulose column fraction (IIA). Fraction IIA contains DNA polymerase alpha-primase and DNA polymerase delta. Both of these polymerases are essential for papillomavirus DNA replication in vitro. However, unlike the case with T-antigen-dependent replication from the simian virus 40 origin, purified DNA polymerase alpha-primase and delta cannot efficiently replace fraction IIA in the replication reaction. Hence, additional cellular factors seem to be required for papillomavirus DNA replication. ...
Sarcoids were diagnosed in two captive zebras from different facilities. Zebra 1 (Equus burchellii boehmi) was a 4.5-yr-old, captive-born male that presented with a 9- by 7-cm inguinal mass. Seven months after surgical excision of the inguinal mass, the zebra presented with a similar lesion in the right upper eyelid that has relapsed repeatedly and has not responded to treatment including local cisplatin injections and cryosurgery. Zebra 2 (of undetermined taxon) was housed at a private wild animal farm. The zebra presented with a single, raised, 2.5- by 2.0- by 2.0-cm, ulcerated mass on the nose, and surgical excision was curative. Histologically, the three masses consisted of a dermal, compact, nonencapsulated, poorly demarcated neoplasm composed of well-differentiated spindle cells arranged in streams and whorls and accompanied by moderate epidermal hyperplasia with long rete pegs. On the basis of the morphologic resemblance to the unique equine cutaneous neoplasm, sarcoid was diagnosed. ...
To study the interaction of E2 with specific regions of the mitotic chromosome in more detail, we developed a method to spread the mitotic chromosomes in situ that was compatible with indirect immunofluorescence for the E2 protein. This method was adapted from that of Smith et al., who used it to localize tankyrase on human telomeres (27). This technique resulted in well-spread mitotic chromosomes and demonstrated that the wild-type E2-TA was localized on individual chromosomes as speckles, whereas E2-TR was excluded from chromosomes. This technique disrupted the perichromosomally associated proteins Ki67 and topoisomerase II but did not affect the chromosomal association of phosphorylated histone H3 (data not shown). This indicates that the E2 protein is tightly bound to mitotic chromosomes. In fact, in other studies from our laboratory we find that E2-TA forms a very stable complex with mitotic chromosomes that is resistant to high salt (M. McPhillips, K. Ozato, and A. A. McBride, submitted ...
The epidemiology of cervical cancer indicates the presence of a sexually transmitted risk factor, attributable at least in part to infection with human papillomavirus (HPV) type 16 or 18. We performed a seroepidemiological study of HPV and cervical cancer in the counties of Västerbotten and Norrbotten in Northern Sweden, a low-risk area for cervical cancer. Sera from 94 cases of incident cervical cancer were matched against 188 age- and sex-matched controls derived from a population-based blood bank. IgG and IgA antibodies were measured against a panel of 12 antigens derived from HPV types 6, 11,1 6, or 18, as well as against Herpes simplex virus type 1 and 2, Chlamydia trachomatis, cytomegalovirus, Epstein-Barr virus, and bovine papillomavirus. Significantly increased relative risks (RRs) were found for IgG to HPV 16- or 18-derived antigens from the L1 (RR = 3.1), E2 (RRs = 2.8 and 9.2), and E7 (RRs = 3.8 and 2.7) open reading frames and for IgA to HPV 16-derived antigens from the E2 (RR = ...
The goal of stable, long-term transfection is to isolate and propagate individual clones containing transfected DNA that has integrated into the cellular genome. Distinguishing nontransfected cells from those that have taken up exogenous DNA involves selective screening. This screening can be accomplished by drug selection when an appropriate drug-resistance marker is included in the transfected DNA. Alternatively, morphological transformation can be used as a selectable trait in certain cases. For example, bovine papilloma virus vectors produce a morphological change in transfected mouse CI127 cells.. Before using a particular drug for selection purposes, you will need to determine the amount of drug necessary to kill untransfected cells. This may vary greatly among cell types. Consult Current Protocols in Molecular Biology for additional information about designing experiments to test various drug concentrations and determine the amount needed to select resistant clones (i.e., generate a kill ...
Wolf, M, Garcea RL, Grigorieff N, Harrison SC. 2010. Subunit interactions in bovine papillomavirus. Proc Natl Acad Sci U S A. 107(14):6298-6303. Abstract ...
Bovine papilloma antigen detected by fluorescent antibody was correlated with the appearance of infectious virus and with epithelial hyperplasia and hyperkeratinization in the developing bovine cutaneous papilloma. Neither antigen nor infectious virus was found in the earliest stages of growth characterized by connective tissue proliferation and by little or no epithelial change. The cellular localization of papilloma virus antigen in nuclear material of the superficial epithelium was the same when first observed as it was in the well developed wart. Regression of the warts was accompanied by a decrease in specific antigen. Marked variations in susceptibility of individual calves to bovine papilloma virus were observed, as well as differences in the histologic development of warts produced by virus administered intradermally and by scarification.. ...
Seventy six formalin-fixed paraffin-embedded sarcoids from 62 Australian horses, collected over a ten year period, were examined for the presence of genomic sequences from bovine papillomavirus 1 and 2 (BPV1, BPV2) with the polymerase chain reaction (PCR). Sequences that could be amplified by primers specific for BPV1 and BPV2 were present in 56 of the 76 sarcoids (73%). A restriction site present in BPV1 and absent from BPV2 was detected in 28 of 34 amplified products that were treated with endonuclease ...
Koch, C; Ramsauer, A S; Drögemüller, M; Ackermann, M; Gerber, V; Tobler, K (2018). Genomic comparison of bovine papillomavirus 1 isolates from bovine, equine and asinine lesional tissue samples. Virus Research, 244:6-12.. Kiefer, C; Tobler, K; Ramsauer, A C; Biegel, U; Kuehn, N; Ruetten, M (2017). Feline sarcoid in a 1-year-old domestic short-haired cat caused by bovine papillomavirus type 14 in Switzerland. Schweizer Archiv für Tierheilkunde, 159(9):487-491.. Lange, C E; Tobler, K; Lehner, A; Grest, P; Welle, M M; Schwarzwald, C C; Favrot, C (2013). EcPV2 DNA in equine papillomas and in situ and invasive squamous cell carcinomas supports papillomavirus etiology. Veterinary Pathology, 50(4):686-692.. Lange, C E; Vetsch, E; Ackermann, M; Favrot, C; Tobler, K (2013). Four novel papillomavirus sequences support a broad diversity among equine papillomaviruses. Journal of General Virology, 94(Pt 6):1365-1372.. Lange, C E; Tobler, K; Lehner, A; Vetsch, E; Favrot, C (2012). A case of a canine ...
TY - JOUR. T1 - Molecular cloning, analysis, and chromosomal localization of a mouse genomic sequence related to the human papillomavirus type 18 E5 region. AU - Kahn, Tomas. AU - Friesl, Holger. AU - Copeland, Neal G.. AU - Gilbert, Debra J.. AU - Jenkins, Nancy A.. AU - Gissmann, Lutz. AU - Kramer, Judith. AU - Hausen, Harald Zur. PY - 1992/1/1. Y1 - 1992/1/1. N2 - The E5 open reading frame (ORF) from bovine papillomavirus type 1 (BPV 1) as well as the E5 ORFs from human papillomaviruses (HPV) type 6 and type 16 have been reported to transform immortalized rodent cells. In an analysis of murine and human tumors for the presence of putative papillomavirus-related sequences, we cloned amplified cellular sequences from the mouse cell line Eb that cross-hybridized with the E5 ORF of HPV 18. A 2.1-kb fragment termed HC1 was sequenced. In normal murine cells, it was present as a single-copy genomic sequence located on chromosome 8. A region of 213 nucleotides corresponded to the E5 gene (HC1 E5), ...
The role of enzymes involved in polycomb repression of gene transcription has been studied extensively in human cancer. Polycomb repressive complexes mediate oncogene-induced senescence, a principal innate cell-intrinsic tumor suppressor pathway that thwarts expansion of cells that have suffered oncogenic hits. Infections with human cancer viruses including human papillomaviruses (HPVs) and Epstein-Barr virus can trigger oncogene-induced senescence, and the viruses have evolved strategies to abrogate this response in order to establish an infection and reprogram their host cells to establish a long-term persistent infection. As a consequence of inhibiting polycomb repression and evading oncogene induced-senescence, HPV infected cells have an altered epigenetic program as evidenced by aberrant homeobox gene expression. Similar alterations are frequently observed in non-virus associated human cancers and may be harnessed for diagnosis and therapy.
Human Papilloma virus type 16 E6 antibody for WB. Anti-Human Papilloma virus type 16 E6 pAb (GTX132686) is tested in Human papillomavirus samples. 100% Ab-Assurance.
Introduction Bovine papillomavirus (BPV) is the etiological agent of bovine papillomatosis, infectious and neoplastic disease, characterized by the presence of multiple papillomas that can regress spontaneously or to persist and progress to malignancies when in association with environmental cofactors. Although recognized that the BPV can induce DNA damages, the viral role following cancer initiation remains unresolved. Based on this, we stablished cell lines derived from cutaneous papilloma, fibropapilloma and esophageal carcinoma to study the BPV action on epithelial-mesenchymal transition (EMT). Our results showed strong evidences that the virus action can contribute to EMT and, therefore, metastasis. Aim In this study, we analyzed the expression levels of the EMT markers (cytokeratin 10, STAT3 Y705, Oct-3/4 and vimentin) in paraffin-embed samples, using the same tissues that originated the cell lines previous studied, aiming to validate the results observed using cell lines. Material and Methods
Learn about the veterinary topic of Equine Sarcoids. Find specific details on this topic and related topics from the Merck Vet Manual.
The skin of animals of a laboratory strain of Mastomys natalensis carrying endogenous, latent papillomavirus genomes was irritated by scratching with glasspaper. Hyperproliferation of the epidermis and amplification of viral DNA followed this treatment, and in approximately 27% of the animals virus-producing papillomas were induced.
These benign but bothersome tumors are as individual as the array of potential treatments. Theyre the most common type of skin tumor in horses, and theyre usually not all that alarming-but although theyre categorized as benign, sarcoids have plenty of troublesome potential.. The thing about sarcoids is, theyre devilishly difficult to defeat. Although they seem to have a common cause-a virus called bovine papilloma, which causes warts in cows-they are masters of disguise, taking any number of different forms and casually defying any kind of easy cure. Not all sarcoids need curing, of course. When they appear in non-troublesome places on the horses skin, and just sit there doing nothing in particular, they are better left alone. But sarcoids that sprout in more troublesome locations-such as the eyelid, the ear, or any spot where tack or equipment might rub or chafe-can cause more than their fair share of grief and need to be dealt with.. Research has been done on sarcoid tumors in horses and ...
BpV(HOpic) is a potent and selective inhibitor of PTEN with an IC50 of 14 nM. Nanocarrier-BpV(HOpic) has neuroprotective activity. - Mechanism of Action & Protocol.
Full Text AI-92-08 PAPILLOMAVIRUS IN VITRO CELL CULTURE SYSTEMS NIH GUIDE, Volume 21, Number 36, October 9, 1992 RFA: AI-92-08 P.T. 34 Keywords: Viral Studies (Virology) Cell Lines Chemotherapeutic Agents National Institute of Allergy and Infectious Diseases Letter of Intent Receipt Date: November 6, 1992 Application Receipt Date: December 10, 1992 PURPOSE The Antiviral Research Branch of the Division of Microbiology and Infectious Diseases (DMID), National Institute of Allergy and Infectious Diseases (NIAID), invites Cooperative Agreement applications from organizational entities willing to participate, with the assistance of the NIAID, in furthering innovative in vitro approaches to the study of papillomavirus infections and their therapeutic control. The goals of this solicitation are to stimulate the use of in vitro papillomavirus replication systems for research on (1) the events of papillomavirus replication and pathogenesis and (2) the antiviral potential of experimental therapeutic ...
La raison pour laquelle ta gynéco en a parlé p- e, c est que les femmes qui veulent tomber enceinte doivent souvent passer un papillomavirus tomber enceinte d infection de chlamydia et autre, c est une contrôle, c est normal. Cette bactérie est responsable de l urétrite à Chlamydia ou chlamydiosemaladie sexuellement transmissible qui est la plus fréquente en Amérique latine et aux États- Unis après le Papillomavirus 50 papillomavirus empeche de tomber enceinte plus fréquente que la gonorrhée, elle- même plus fréquente avoir le papillomavirus enceinte la syphilis.
Orplatna devait être papillomavirus traitement alternatif remede naturel contre le papillomavirus naturel pour traiter le cancer de la remede naturel contre le papillomavirus. Traducerile vulgare sau colocviale sunt în general marcate cu roșu sau cu portocaliu. Specialistul gastroenterolog Silva Cesar Pedrazas a acceptat invitația noastră și ne- a explicat, pe înțelesul tuturor, cât de importantă este colonoscopia remede papillomavirus homme sănătatea noastră.
A total number of 159 equine cases in different regions of Egypt were surveyed. The survey consisted of 83 horses and 76 donkeys of different ages affected wit...
Infuzie din rădăcină - se prepara din o linguriţă de rădăcină la o cană cu apă, se pot zenzero e papillomavirus 2 căni pe zi. Cancerul zodia hpv high risk who, squamous papilloma virus chello webmail. Rahim Ağzı Kanseri Nedir?
HPV has been linked with an increased risk of cardiovascular disease. Papillomavirus humain oncogene, Non hodgkins lymphoma cancer Comprimate antiparazitare de la demodex Anumite tulpini infectează colul uterin și au fost implicate ca o cauză a cancerului de col uterin HPV. In faza IE sunt activate un numar foarte restans de gene virale IE, care vor codifica sinteza unor proteine virale functionale ce vor activa transcrierea genelor E.
Nu sunt selectate sau validate papillomavirus homme verrue, așadar pot conține termeni inadecvați. Ce este pre-cancer si ce inseamna CIN?
[196 Pages Report] Check for Discount on Global Papillomavirus Diagnostics Market Country Shares and Segment Forecasts 2017-2021: Emerging Opportunities and Growth Strategies for Suppliers report by Venture Planning Group. The report presents a detailed analysis of the Papillomavirus diagnostics...
Din pacate aceste forme nu determina semne sau simptome decat in stadii tardive ale cancerelor respective, ceea ce le face sa fie dificil de tratat si vindecat.
CONICET Digital, el repositorio institucional del CONICET, un servicio gratuito para acceder a la producción científico-tecnológica de investigadores, becarios y demás personal del CONICET.
Un alt cancer produs de infectia cu HPV este cancerul vaginal care papillomavirus femme transmission cel mai des la femeile de peste 60 de ani. Cnd este superficial este de culoare roie-albstrie; localizarea profund este pe bulbi sau corpii cavernoi clitoridieni.
L hépatite B est provoquée par un virus qui s attaque au foie. Atunci îi putem mulțumi iubitului tău pentru asta. Cest linterrogatoire du patient qui permet dorienter le diagnostic.
He who smokes is unfit for the practice of Karma Yoga. He becomes dull when he cannot get a whiff of smoke. He cannot work in the absence of cigarettes. He wastes his money that can be very well utilised in the service of others. A Karma Yogi should be strictly free from the evil habit of smoking.. Smoking is an evil habit. Smokers bring a little bit of philosophy and medical opinion in support of their principles. They say: Smoking keeps my bowels free. I get a good motion in the morning. It is very exhilarating to the lungs, brain and the heart. When I sit for meditation after a smoke, I meditate better. Why should I give it up? Very sound philosophy indeed! They bring in ingenious arguments to support their evil habit. They cannot get rid of this evil habit. They are heavy smokers who can finish a packet of cigarettes within a few hours. This habit starts from early boyhood. A little boy removes a cigarette from his elder brothers pocket and tries a first smoke. He gets a little tickling ...
A highly sensitive, automated, purely add-on, high-throughput pseudovirion-based neutralization assay (HT-PBNA) with excellent repeatability and run-to-run reproducibility was developed for human papillomavirus types (HPV) 16, 18, 31, 45, 52, 58 and bovine papillomavirus type 1. Preparation of 384 well assay plates with serially diluted sera and the actual cell-based assay are separated in time, therefore batches of up to one hundred assay plates can be processed sequentially. A mean coefficient of variation (CV) of 13% was obtained for anti-HPV 16 and HPV 18 titers for a standard serum tested in a total of 58 repeats on individual plates in seven independent runs. Natural antibody response was analyzed in 35 sera from patients with HPV 16 DNA positive cervical intraepithelial neoplasia grade 2+ lesions. The new HT-PBNA is based on Gaussia luciferase with increased sensitivity compared to the previously described manual PBNA (manPBNA) based on secreted alkaline phosphatase as reporter. Titers ...
An avian papillomavirus genome has been cloned from a cutaneous exophytic papilloma from an African grey parrot (Psittacus erithacus). The nucleotide sequence, genome organization, and phylogenetic position of the Psittacus erithacus papillomavirus (PePV) were determined. This PePV sequence represents the first complete avian papillomavirus genome defined. The PePV genome (7304 basepairs) differs from other papillomaviruses, in that it has a unique organization of the early protein region lacking classical E6 and E7 open reading frames. Phylogenetic comparison of the PePV sequence with partial E1 and L1 sequences of the chaffinch (Fringilla coelebs) papillomavirus (FPV) reveals that these two avian papillomaviruses form a monophyletic cluster with a common branch that originates near the unresolved center of the papillomavirus evolutionary tree. The PePV genome has a unique layout of the early protein region which represents a novel prototypic genomic organization for avian papillomaviruses. The close
The precise aetiology of enzootic haematuria in cattle remains unknown. The involvement of bracken fern (Pteridium aquilinium) appears certain because of the close association between bracken fern infested farms and enzootic haematuria. Several toxic principles have been identified but the main carcinogenic element remains to be conclusively demonstrated. More recently, bovine papilloma virus has been implicated in the aetiology of enzootic haematuria. Its possible interaction with bracken fern carcinogen is discussed.. ...
In 2011, Rock had a sarcoid on his neck in front of the withers where there was a lot of fatty tissue and the skin was loose enough to pull the sarcoid away from the body. So, we shaved his neck and removed the sarcoid with surgery. We then had it biopsied to find it was not a serious sarcoid (Better to be safe than sorry!) and it eventually just went away. In the eighties, if we removed a sarcoid, it would have had a follow-up of injections to be completely rid of it. In the nineties, veterinarians discovered another way to treat sarcoids that involved taking a piece of the biopsied sarcoid and reintroducing it as an implant in the neck to prompt an immunity response. Before he could remove one of three sarcoids the from Lucky Three Eclipse, he rubbed one and tore it open. Before we had the chance to biopsy one of the sarcoids for an implant, as if a miracle, his immune system was stimulated by HIM, kicked in and all three sarcoids just disappeared…and no, they were not anything else.. ...
În plus, infecțiile cu HPV 16 și 18 sunt o cauză a unui tip unic de cancer orofaringian. Anumite tulpini infectează colul uterin și au fost implicate ca o cauză a cancerului de col uterin HPV.
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Beginning in 2012, our understanding of human papillomavirus (HPV) subcellular trafficking has undergone a drastic paradigm shift. Work from multiple laboratories has revealed that HPV has evolved a unique means to deliver its viral genome (vDNA) to the cell nucleus, relying on a myriad of host cell proteins and processes. The major breakthrough finding from these recent endeavors was the realization of L2-dependent utilization of cellular sorting factors for the retrograde transport of vDNA away from degradative endo/lysosomal compartments to the Golgi, prior to mitosis-dependent nuclear accumulation of L2/vDNA. An overview of current models of HPV entry, subcellular trafficking, and the role of L2 during initial infection is provided below, highlighting unresolved questions and gaps in knowledge.
Wart under foot hurts hpv skin warts treatment, papilloma virus vaccinazione maschi cancer pulmonar y tabaquismo. Prezentarea în ordine alfabeticã, com- În fine, veþi gãsi de asemenea: pletatã de douã indexuri - unul pe ca- - un mic vaccin papillomavirus et maladie de verneuil ilustrat al celor mai tegorii de boli ºi unul general, situate la frecvente afecþiuni ale pielii semnala- sfîrºitul cãrþii - faciliteazã accesul la rea se face printr-un D urmat de informaþia doritã.
Hargrove, Stacy A.; Work, Thierry M.; Brunson, Shandell; Foley, Allen Michael; Balazs, George H.; Girard, Alexandre; Stacy, Brian A. (Brian Adams); Diez, Carlos; Baptistotte, Cecilia; Limpus, Colin James; Walsh, Daniel; Lynch, Jennifer; Jones, Karina; Ehrhart, Llewellyn; Chaloupka, Milani; Van Houtan, Kyle Schuyler, 1974-; Murakawa, Shawn K. K ...
Bakgrunn: Infeksjon med humant papillomavirus er den vanligste seksuelt overførbare infeksjonen både hos kvinner og menn. Det er anslått at opptil 20 % av den seksuelt aktive befolkningen på et hvert tidspunkt er smittet, og at over 70 % av befolkningen vil få en HPV- infeksjon i løpet av sitt seksuelt aktive liv. Høsten 2009 ble vaksinen mot humant papilloma virus (HPV) innført i barnevaksinasjonsprogrammet for jenter på 7. klassetrinn. Som for alle vaksiner i barnevaksinasjonsprogrammet er dette et tilbud man kan velge å ta i mot. Siden denne vaksinen er ny, vil det være usikkerhet knyttet til i hvor stor grad foreldrene vil tillate at vaksinen anvendes på døtrene. I denne forbindelse har jeg valgt å undersøke vurderinger foreldrene gjør for å bestemme seg om de skal la døtrene sine vaksineres eller ikke. Material og metode: Denne oppgaven er et litteraturstudium. I utarbeidelsen har jeg brukt søkeportalen PubMed. I samarbeid med veileder har jeg funnet de mest relevante ...
The aim of this study was to investigate microRNA (miRNA) differential expression in the two most common equine skin tumours, equine sarcoid (ES) and squamous cell carcinoma (SCC), and its potential influence on the tumour microenvironment at post‐transcriptional level. We investigated miRNA fingerprints in four subgroups: mild (ESM) and aggressive (ESA) ES and ocular SCC (oSCC) and genital SCC (g ...
Parce que de nombreux organismes sont situés dans le bassin, la chirurgie pour enlever les tumeurs de grande taille nécessitent la dépose de ces organes.
Infecţia cu virusul papiloma uman HPV este principala cauză. All these efforts in terms of public funds and the cured persons mobilization did not succeed to change the incidence and the mortality by cervical cancer.
Apr 1 ; 7 E6 binds to p53 via a cellular ubiquitin ligase named E6AP, so that it becomes ubiquitinated, leading to degradation and down-regulation of pathways involved in cycle arrest and apoptosis.
mouse C9orf10 protein: An accessory role of C9orf10 for Puralpha in a limited brain region in addition to other possible functions that have not yet been determined.
A public health laboratory in Nevada has reported the first confirmed coronavirus reinfection in the United States, and the first in the world known to have brought on severe symptoms.. The first three confirmed reinfections in the world were reported this week, one in Hong Kong and two in Europe, all mild.. Reinfection does not surprise researchers, given the millions of cases around the world, but it is not yet clear if such cases - particularly severe ones - are anomalies or will prove common.. The patient is a 25-year-old man in Reno who apparently experienced a second bout of infection just 48 days after his first, according to health officials in Nevada.. Experts have said that even low levels of antibodies and T cells in response to infection should last for a few months and provide some protection against the virus, which appears to have been borne out in the other confirmed reinfections.. The patient in Nevada had a sore throat, cough, nausea and diarrhea starting on March 25. He tested ...
1980 Law, M. F.; Lowy, D. R.; Dvoretzky, I.; Howley, P. M. (1981). "Mouse cells transformed by bovine papillomavirus contain ... 1995 Tong, X.; Howley, P. M. (1997). "The bovine papillomavirus E6 oncoprotein interacts with paxillin and disrupts the actin ... 1982 Yang, Y. C.; Okayama, H.; Howley, P. M. (1985). "Bovine papillomavirus contains multiple transforming genes". Proceedings ... "In vitro tumorigenic transformation by a defined sub-genomic fragment of bovine papilloma virus DNA". Nature. 287 (5777): 72-74 ...
Schiller, J. T.; Vass, W. C.; Vousden, K. H.; Lowy, D. R. (1986). "E5 open reading frame of bovine papillomavirus type 1 ... Lechner, M. S.; Mack, D. H.; Finicle, A. B.; Crook, T.; Vousden, K. H.; Laimins, L. A. (1992). "Human papillomavirus E6 ... Vousden's early work focused on the molecular biology of human papillomaviruses (HPVs), which are associated with cervical ... she led the Human Papillomavirus Group at the Ludwig Institute for Cancer Research, London, UK. In 1995, she joined the ...
Cattle serve as natural hosts and it is one of the bovine papillomaviruses. There are currently two species in this genus, ... Bovine serve as the natural host. Transmission routes are contact. Van Doorslaer, K; Chen, Z; Bernard, HU; Chan, PKS; DeSalle, ... Group: dsDNA Order: Zurhausenvirales Family: Papillomaviridae Genus: Epsilonpapillomavirus Epsilonpapillomavirus 1 ... including the type species Epsilonpapillomavirus 1. Diseases associated with this genus include: fibropapillomas and true ...
Crystal structure at 1.7 Å of the bovine papillomavirus-1 E2 DMA-binding domain bound to its DNA target. Nature 359, 505-512 (8 ... His interests are cervical cancer, papillomaviruses and epithelia cells. His highest cited paper is Human papillomavirus ... Pathogenesis of Human Papillomaviruses in Differentiating Epithelia. Microbiol. Mol. Biol. Rev. June 2004 vol. 68 no. 2 362-372 ... Biosynthesis of human papillomavirus from a continuous cell line upon epithelial differentiation. Science Vol. 257, Iss. 5072 ...
Vande Pol SB, Brown MC, Turner CE (January 1998). "Association of Bovine Papillomavirus Type 1 E6 oncoprotein with the focal ... 293 (1): 38-52. doi:10.1016/j.ydbio.2005.12.040. PMID 16533505. Gehmlich K, Pinotsis N, Hayess K, van der Ven PF, Milting H, El ... 513 (1): 114-8. doi:10.1016/s0014-5793(01)03244-6. PMID 11911889. S2CID 26269466. Salgia R, Li JL, Lo SH, Brunkhorst B, Kansas ... 4 (1): 146-52. doi:10.4161/cam.4.1.10973. PMC 2852571. PMID 20139696. Wood CK, Turner CE, Jackson P, Critchley DR (February ...
"Roles of the hinge region and the DNA binding domain of the bovine papillomavirus type 1 E2 protein in initiation of DNA ... 75 (1): 128-37. doi:10.1086/422196. PMC 1181996. PMID 15162323. Qamar R, Ayub Q, Mohyuddin A, Helgason A, Mazhar K, Mansoor A, ... 54 (1): 47-55. doi:10.1038/jhg.2008.2. PMID 19158816. S2CID 22162114. Qamar R, Ayub Q, Mohyuddin A, Helgason A, Mazhar K, ... ISBN 978-1-4613-6914-1. Baig MM, Khan AA, Kulkarni KM (September 2004). "Mitochondrial DNA diversity in tribal and caste groups ...
Several studies have found an association between the presence of Bovine papillomavirus-1 and 2 and associated viral growth ... "Association of bovine papillomavirus with the equine sarcoid". Journal of General Virology. 84 (5): 1055-1062. doi:10.1099/vir. ... "Intralesional bovine papillomavirus DNA loads reflect severity of equine sarcoid disease". Equine Veterinary Journal. 42 (4): ... Equine papillomavirus-2 has also been found within penile SCCs, but has not been determined to cause SCC. Before treatment of ...
The E5 protein of some animal papillomavirus types (mainly bovine papillomavirus type 1) functions as an oncogene primarily by ... Only a few papillomavirus types encode a short protein from the E8 gene. In the case of BPV-4 (papillomavirus genus Xi), the E8 ... Jackson ME, Pennie WD, McCaffery RE, Smith KT, Grindlay GJ, Campo MS (1991). "The B subgroup bovine papillomaviruses lack an ... Inter-species transmission has also been documented for bovine papillomavirus (BPV) type 1. In its natural host (cattle), BPV-1 ...
With Bill, she and her colleagues investigated in detail the biology of bovine papillomaviruses, especially BPV-1, -2 and -4, ... bovine papillomavirus type 4 (BPV-4). In older cattle, he noted that cancers could develop from existing papillomas and ... These results laid the conceptual framework for the production of vaccines against the subtypes of human papillomaviruses that ... HTLV-1), and later human immunodeficiency virus (HIV). With substantial funding from cancer charities, Bill then recruited a ...
Battlefield Planning Visualization Bovine papillomavirus BQ (s) British Antarctic Territory (former ISO 3166 digram; merged ... Bovine Spongiform Encephalopathy, better known as mad cow disease BSE - (i) Buku Sekolah Elektronik (screw thread), BSF - (i) ... Bovine somatotropin (i) British Summer Time BSW - (i) British Standard Whitworth (screw thread) BSX - (i) Bendigo Stock ... ISO 639-1 code) Ba - (s) Barium BA - (i) Bachelor of Arts (s) Bahrain (FIPS 10-4 country code) Bosnia and Herzegovina (ISO 3166 ...
Similar papillomaviruses of ungulates (e.g. deer papillomavirus, European elk papillomavirus, ovine papillomavirus 1,2) are ... Campo, MS (2006). "Bovine papillomavirus: old system, new lessons?". In Campo, MS (ed.). Papillomavirus Research: From Natural ... Chambers G, Ellsmore VA, O'Brien PM, Reid SW, Love S, Campo MS, Nasir L (2003). "Association of bovine papillomavirus with the ... Bovine papillomaviruses (BPV) are a paraphyletic group of DNA viruses of the subfamily Firstpapillomavirinae of ...
Allshire, Robin Campbell (1985). Construction and analysis of vectors based on bovine papilloma virus (PhD thesis). University ... 21 (1 Suppl): 5-9. doi:10.1038/4429. PMID 9915493. S2CID 2690775. Southern, E. (1979). "[9] Gel electrophoresis of restriction ...
"Bovine leukemia virus linked to breast cancer but not coinfection with human papillomavirus: Case-control study of women in ... Bovine leukemia virus (BLV) is a retrovirus which causes enzootic bovine leukosis in cattle. It is closely related to the human ... Bovine leukaemia virus RNA packaging signal "ICTV Taxonomy history: Bovine leukemia virus". International Committee on Taxonomy ... Meas S, Usui T, Ohashi K, Sugimoto C, Onuma M (January 2002). "Vertical transmission of bovine leukemia virus and bovine ...
... papillomavirus MeSH B04.280.535.600.650 - papillomavirus, bovine MeSH B04.280.535.600.660 - papillomavirus, cottontail rabbit ... papillomavirus MeSH B04.909.204.210.655.600.650 - papillomavirus, bovine MeSH B04.909.204.210.655.600.660 - papillomavirus, ... papillomavirus MeSH B04.909.574.204.655.600.650 - papillomavirus, bovine MeSH B04.909.574.204.655.600.660 - papillomavirus, ... papillomavirus MeSH B04.909.624.600.650 - papillomavirus, bovine MeSH B04.909.624.600.660 - papillomavirus, cottontail rabbit ...
Fibropapillomas are present in other animal groups, but are caused by different viruses, for example the bovine papillomavirus ... 2012). "A Histopathological, Immunohistochemical and Molecular Study of Cutaneous Bovine Papillomatosis". Kafkas Univ Vet Fak ... 7 (1): 114-125. doi:10.1016/j.hal.2007.06.001. Tan, M. T.; Yildirim, Y.; Sozmen, M.; Bilge-Dagalp, S.; Yilmaz, V.; et al. ( ... 41 (1): 29-41. doi:10.7589/0090-3558-41.1.29. PMID 15827208. Quackenbush, S. L.; Casey, R. N.; Murcek, R. J.; Paul, T. A.; Work ...
You J, Croyle JL, Nishimura A, Ozato K, Howley PM (Apr 2004). "Interaction of the bovine papillomavirus E2 protein with Brd4 ... Abbate EA, Voitenleitner C, Botchan MR (Dec 2006). "Structure of the papillomavirus DNA-tethering complex E2:Brd4 and a peptide ... Baxter MK, McPhillips MG, Ozato K, McBride AA (Apr 2005). "The mitotic chromosome binding activity of the papillomavirus E2 ... Schweiger MR, You J, Howley PM (May 2006). "Bromodomain protein 4 mediates the papillomavirus E2 transcriptional activation ...
"Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication". Journal of ... Kaldalu N; Lepik D; Kristjuhan A; Ustav M. (2000). "Monitoring and purification of proteins using bovine papillomavirus E2 ... Icosagen's main products are: FITkit (latex allergen test) and FITkit testing service [1] E2Tag epitope tagging technology MAbs ...
... worked with Daniel DiMaio on the interactions between platelet-derived growth factor receptors and the bovine papillomavirus E5 ... 231 (1): 265-278. doi:10.1006/dbio.2000.0135. ISSN 0012-1606. PMID 11180967. LaFever, Leesa; Drummond-Barbosa, Daniela (2005-08 ...
Pseudocowpox Warts caused by bovine papillomavirus Teat-end hyperkeratosis Dermatitis Frostbite Udder sores or necrotic ... ISBN 978-0-13-046256-5. Ruegg, Pamela L. "Diseases of Bovine Teats and Skin - Reproductive System". Merck Veterinary Manual. ... ISBN 978-1-4615-4937-6. "With the Wild Things - Transcripts". Digitalcollections.fiu.edu. Archived from the original on 2013-03 ... Rowen D. Frandson; W. Lee Wilke; Anna Dee Fails (1 April 2013), Anatomy and Physiology of Farm Animals, John Wiley & Sons, pp. ...
Construction and analysis of vectors based on bovine papilloma virus (PhD thesis). University of Edinburgh. hdl:1842/11176. ... obtained his PhD in 1985 under the guidance of Chris Bostock and Edwin Southern investigating the use of bovine papillomavirus ... 76 (1): 157-69. doi:10.1016/0092-8674(94)90180-5. PMID 8287474. Allshire, R. C; Nimmo, E. R; Ekwall, K; Javerzat, J. P; ...
deer papillomavirus. *European elk papillomavirus. *bovine papillomavirus - 1. *Epsilonpapillomavirus *bovine papillomavirus - ... Human papillomavirus, HPV findes i over hundrede forskellige arter. De kan bl.a være årsag til vorter, kønsvorter og ... Man regner med at HPV er den mest almindeligt seksuelt overførte virus.[1] ...
The bacterium has high correlations with the Human Papillomavirus (HPV).[citation needed] It has also been linked to ... bovine) during the Cretaceous period. It was found that the most closely related species strain of Ureaplasma to Ureaplasma ... 234 (1): 100-9107. doi:10.1002/jcp.26952. PMID 30078192. Hillitt, K. L.; Jenkins, R. E.; Spiller, O. B.; Beeton, M. L. (2017 ... urealyticum was Ureaplasma diversum (isolated from bovine). U. urealyticum can cause urethritis and may cause bacterial ...
"Human Papillomavirus (HPV) and Cancer , CDC". 2019-08-21. Münger K, Baldwin A, Edwards KM, Hayakawa H, Nguyen CL, Owens M, et ... Buehring GC, Shen HM, Jensen HM, Jin DL, Hudes M, Block G (2 September 2015). "Exposure to Bovine Leukemia Virus Is Associated ... In Western developed countries, human papillomavirus (HPV), hepatitis B virus (HBV) and hepatitis C virus (HCV) are the most ... Closely related to human T-cell leukemia virus, is another deltaretrovirus, bovine leukemia virus (BLV), which recently has met ...
The best-studied example is the association between Human papillomavirus and cervical cancer: almost all cases of cervical ... Creutzfeldt-Jakob disease and bovine spongiform encephalopathy ("mad cow disease"), are caused by prions, hepatitis D is due to ... Harald zur Hausen had shown that two strains of Human papillomavirus (HPV) cause most cases of cervical cancer. Two vaccines ... "1 Moved , Prion Diseases , CDC". Archived from the original on 2010-03-04. Retrieved 2017-09-17. Dimmock NJ, Easton AJ, Leppard ...
See also: Papillomavirus and Bovine papillomavirus. This section is empty. You can help by adding to it. (December 2017) ... Warts are caused by the human papilloma virus (HPV). There are about 130 known types of human papilloma viruses.[8] HPV infects ... a b Human Papillomavirus Archived 2015-08-23 at the Wayback Machine.. Public Health Agency of Canada ... Warts are caused by infection with a type of human papillomavirus (HPV).[1] Factors that increase the risk include use of ...
Such benefits include male insects transferring nutrients to females via their ejaculate; in both humans and bovines, the fluid ... This includes transmission risk for sexually transmitted diseases such as human papillomavirus (HPV) or herpes, especially for ... Retrieved 1 December 2016. Rafik, Amal (2013-03-26). Passion Masters: Sex Secrets of a Forbidden Cult. Google Books: Blue Moon ... ISBN 978-1-4615-4937-6. Dewsbury, Donald A. (1972). "Patterns of Copulatory Behavior in Male Mammals". The Quarterly Review of ...
... , Benes L. Trus & Stephen C. Harrison (2002). An atomic model of the papillomavirus capsid. EMBO J., 21, 4754-4762 ... Yue Li, Jimin Wang, Ryuta Kanai & Yorgo Modis (2013). Crystal structure of glycoprotein E2 from bovine viral diarrhea virus. ... IL-13Rα2 uses TMEM219 in chitinase 3-like-1-induced signaling and effector responses. Nat. Commun., 7, 12752. Shmuel Willensky ... Crystal structure of dengue type 1 envelope protein in the postfusion conformation and its implication for receptor binding, ...
The human papillomavirus vaccine is recommended in the U.S. (as of 2011) and UK (as of 2009). Vaccine recommendations for the ... Bovine herpesvirus 1 DIVA vaccines are also widely used in practice.[citation needed] Considerable efforts are ongoing to apply ... "HPV Vaccine , Human Papillomavirus , CDC". www.cdc.gov. 2019-05-13. Retrieved 2019-06-14. Williamson, E. D.; Eley, S. M.; ... In the case of a few relatively new vaccines such as the human papillomavirus vaccine, the patents may impose an additional ...
"Human papillomavirus infection in head and neck cancer: the role of the secretory leukocyte protease inhibitor". Oncology ... crystal structure of the acid-stable proteinase inhibitor from human mucous secretions analysed in its complex with bovine ... it has been shown that there is an inverse correlation between the levels of SLPI and high-risk Human Papillomavirus (HPV) ... "The role of the antileukoprotease SLPI in smoking-induced human papillomavirus-independent head and neck squamous cell ...
The human papillomavirus vaccine is recommended in the U.S. (as of 2011)[63] and UK (as of 2009).[64] Vaccine recommendations ... Bovine herpesvirus 1 DIVA vaccines are also widely used in practice. Other DIVA vaccines (under development). Scientists have ... "HPV Vaccine , Human Papillomavirus , CDC". www.cdc.gov. 2019-05-13. Retrieved 2019-06-14.. ... In the case of a few relatively new vaccines such as the human papillomavirus vaccine, the patents may impose an additional ...
One area of particular interest is the study of human papilloma viruses (HPV) and their role in cervical cancers. Researchers ... Having finally lost completely its virulence, the bovine tuberculosis germ grown with their method was the principal ... he focused on creating a vaccine using the bacillus responsible for bovine tuberculosis, very similar to the human one, as it ... In addition to the isolation of HIV-1 and HIV-2, in the recent past researchers at the Institut Pasteur have developed a test ...
Cervical cancer, vulvar cancer & Genital warts (condyloma), Penile cancer, Anal cancer (Human papillomavirus (HPV)) ... Wart (caused by the Human papillomavirus; also similar in appearance to molluscum) ... 7 (1): 57-62. PMID 10359957.. *^ a b Torbeck R, Pan M, DeMoll E, Levitt J (June 2014). "Cantharidin: a comprehensive review of ... 132 (1): 1-3. doi:10.1542/peds.2013-0116. PMID 23796740.. *^ DailyMed. Aldara (imiquimod) Cream for Topical use (Prescribing ...
Cervical cancer, vulvar cancer & Genital warts (condyloma), Penile cancer, Anal cancer (Human papillomavirus (HPV)) ... 75 (1): 93-100. doi:10.1002/jmv.20242. PMID 15543582.. *^ a b Minhas, V; Crabtree, K. L.; Chao, A; m'Soka, T. J.; Kankasa, C; ... 12 (1): 47-56. doi:10.1097/COH.0000000000000330. PMID 27662501.. *^ Kaposi, M (1872). "Idiopathisches multiples Pigmentsarkom ... This virus causes Kaposi's sarcoma, a cancer commonly occurring in AIDS patients,[1] as well as primary effusion lymphoma,[2] ...
人類乳突病毒(Human Papillomavirus,HPV)是一种DNA病毒,属于乳头瘤病毒科乳头瘤病毒属。該類病毒感染人體的表皮與黏膜組織,目前約有170种類型的HPV被判別出來,有些时候HPV入侵人體後會引起疣甚至癌症,但大多数时候則沒有任何临床 ... Bovine papular stomatitis(英语:Bovine papular stomatitis). *Pseudocowpox(英
Schiffman M, Castle PE, Jeronimo J, Rodriguez AC, Wacholder S. Human papillomavirus and cervical cancer. The Lancet. 2007;370( ... bovine spongiform encephalopathy ("mad cow" disease) in cattle, and chronic wasting disease in deer; in humans, prionic ... de Villiers EM, Fauquet C, Broker TR, Bernard HU, zur Hausen H. Classification of papillomaviruses. Virology. 2004;324(1):17-27 ... Human papillomaviruses are an established cause of cancers of cervix, skin, anus, and penis.[182] Within the Herpesviridae, ...
Human papillomavirus (HPV). *Genital warts (condyloma). *Hepatitis B (Hepatitis B virus). *Herpes simplex *HSV-1 & HSV-2 ... 978-1-59745-040-9. .. *^ a b c d Leone P (2005). "Reducing the risk of transmitting genital herpes: advances in understanding ... 350 (1): 11-20. doi:10.1056/NEJMoa035144. PMID 14702423.. *^ a b Wald A, Langenberg AG, Link K, Izu AE, Ashley R, Warren T, ... 186 (Suppl 1): S3-28. doi:10.1086/343739. PMID 12353183.. *^ Xu, Fujie; Fujie Xu; Maya R. Sternberg; Benny J. Kottiri; ...
Human papillomavirus (HPV) infection One of the Human papillomaviruses Human parainfluenza virus infection Human parainfluenza ... Bovine spongiform encephalopathy. *Camel spongiform encephalopathy. *Scrapie. *Chronic wasting disease. *Transmissible mink ... Cervical cancer, vulvar cancer & Genital warts (condyloma), Penile cancer, Anal cancer (Human papillomavirus (HPV)) ... Dengue viruses (DEN-1, DEN-2, DEN-3 and DEN-4) - Flaviviruses ... Herpes simplex virus 1 and 2 (HSV-1 and HSV-2) Histoplasmosis ...
Bovine herpesvirus 1 DIVA vaccines are also widely used in practice.[citation needed] Considerable efforts are ongoing to apply ... The human papillomavirus vaccine is recommended in the U.S. (as of 2011)[87] and UK (as of 2009).[88] Vaccine recommendations ... "HPV Vaccine , Human Papillomavirus , CDC". www.cdc.gov. 2019-05-13. Retrieved 2019-06-14.. ... In the case of a few relatively new vaccines such as the human papillomavirus vaccine, the patents may impose an additional ...
... is an infection by human papillomavirus (HPV).[4] Most HPV infections cause no symptoms and ... An HPV infection is caused by human papillomavirus, a DNA virus from the papillomavirus family, of which over 170 types are ... "Pink Book (Human Papillomavirus)" (PDF). CDC.gov. Archived (PDF) from the original on 21 March 2017. Retrieved 18 April 2017.. ... "Human Papillomavirus". Medscape. Archived from the original on 29 November 2016.. *^ "Photos of larynx Papillomas - Voice ...
Lebwohl MG; Rosen; Stockfleth (November 2010). "The role of human papillomavirus in common skin conditions: current viewpoints ... B virus infection Boston exanthem disease Bovine papular stomatitis Bowenoid papulosis Buffalopox Butcher's wart Chikungunya ... ISBN 1-4051-3130-6. Paus R, Cotsarelis G (1999). "The biology of hair follicles". N Engl J Med. 341 (7): 491-7. doi:10.1056/ ... ISBN 978-1-4160-3185-7. Lippens S, Hoste E, Vandenabeele P, Agostinis P, Declercq W (April 2009). "Cell death in the skin". ...
... the use of less abundant bovine ribs may indicate a specific preference for bovine ribs. Potential lissoirs have also been ... The modern human papillomavirus variant 16A may descend from Neanderthal introgression. A Neanderthal at Cueva del Sidrón, ... "Transmission between archaic and modern human ancestors during the evolution of the oncogenic human papillomavirus 16". ... Teshik-Tash 1 from Uzbekistan was associated with a circle of ibex horns, and a limestone slab argued to have supported the ...
If biotin was used as a probe label, non-specific binding sites must first be blocked using bovine serum albumin (BSA). Then, ... Apart from cancers, CISH has also been shown to be useful in detecting human papillomavirus infections. SISH uses a similar ... doi:10.1007/978-1-60761-789-1_1. ISBN 978-1-60761-788-4. PMID 20809300. Lambros, M. B.; Simpson, P. T.; Jones, C; Natrajan, R; ... 50 (1): 26-30. doi:10.2302/kjm.50.26. PMID 11296661. Darouich, S; Popovici, C; Missirian, C; Moncla, A (2012). "Use of DOP-PCR ...
3.D.1 The H+ or Na+-translocating NADH Dehydrogenase ("complex I") family 3.D.2 The Proton-translocating Transhydrogenase (PTH ... The upper level of classification and a few examples of proteins with known 3D structure: 1.A.1 Voltage-gated ion channel ... Saier, M. H.; Yen, M. R.; Noto, K.; Tamang, D. G.; Elkan, C. (1 January 2009). "The Transporter Classification Database: recent ... Saier, M. H.; Tran, C. V.; Barabote, R. D. (1 January 2006). "TCDB: the Transporter Classification Database for membrane ...
Specific chromosomal abnormalities characterize fibrosarcomas of bovine papillomavirus type 1 transgenic mice. V Lindgren, M ... In the BPV1.69 line of transgenic mice, the bovine papillomavirus type 1 genome elicits both benign dermal fibroblastic ... Specific chromosomal abnormalities characterize fibrosarcomas of bovine papillomavirus type 1 transgenic mice ... Specific chromosomal abnormalities characterize fibrosarcomas of bovine papillomavirus type 1 transgenic mice ...
To investigate the efficiency of encapsidation of plasmid by papillomavirus virus-like particles (PV VLPs), and the infectivity ... of the resultant PV pseudovirions, Cos-1 cells were transfected with... ... Efficiency of delivery of DNA to cells by bovine papillomavirus type-1 L1/L2 pseudovirions. ... To investigate the efficiency of encapsidation of plasmid by papillomavirus virus-like particles (PV VLPs), and the infectivity ...
Bovine Papilloma Virus Type 1 E2 Monoclonal Antibody from Invitrogen for Immunofluorescence, Immunocytochemistry and ELISA ... Supplied as 100 ug purified antibody (1 mg/ml) in PBS with 50% glycerol and no preservative. ... Cite Bovine Papilloma Virus Type 1 E2 Monoclonal Antibody (3E8). The following antibody was used in this experiment: Bovine ... View (7) other Bovine Papilloma Virus Type 1 E2 antibodies Invitrogen Bovine Papilloma Virus Type 1 E2 Monoclonal Antibody (3E8 ...
Anogenital type human papillomavirus (HPV) E6 oncoproteins and E6 from bovine papillomavirus type 1 (BE6) interact with ... Papillomavirus E6 proteins are adapters that change the function of cellular regulatory proteins. The bovine papillomavirus ... Transformation by Bovine Papillomavirus Type 1 E6 Requires Paxillin. Ramon Wade, Nicole Brimer, Scott Vande Pol ... Transformation by Bovine Papillomavirus Type 1 E6 Requires Paxillin Message Subject (Your Name) has forwarded a page to you ...
Bovine papillomavirus type 1 oncogene protein E5: 44-amino acid protein; a homo-dimeric, transmembrane protein that transforms ... Bovine papillomavirus type 1 oncogene protein E5. Subscribe to New Research on Bovine papillomavirus type 1 oncogene protein E5 ... oncogene protein E5, Bovine papillomavirus type 1; E5 protein, Bovine papilloma virus type 1; E5 transforming protein, BPV-1; v ...
Recombinant protein corresponding to full length Bovine Papillomavirus type-1 transactivator protein E2. (MAB7861) - Products ... Mouse monoclonal antibody raised against Bovine papillomavirus type 1 E2. ... Bovine Papillomavirus type 1 E2-protein. Mapped to amino acids 199-208 (SSTSSDFRDR). ... Recombinant protein corresponding to full length Bovine Papillomavirus type-1 transactivator protein E2. ...
Bovine papillomavirus type 1 genomes and the E2 transactivator protein are closely associated with mitotic chromatin. J. Virol. ... In bovine papillomavirus type 1 (BPV1), the E2 gene encodes three different polypeptides (22). The largest protein, expressed ... Conditional Mutations in the Mitotic Chromosome Binding Function of the Bovine Papillomavirus Type 1 E2 Protein. Peng-Sheng ... Bovine papillomavirus mutant temperature sensitive for transformation, replication, and transactivation. EMBO J.7:1197-1204. ...
Campo MS: Bovine papillomavirus: Old system, new lessons?. Papillomavirus Research: From Natural History to Vaccines and Beyond ... Bovine papillomavirus type 1 infects not only cattle but also equids and is a causative factor in the pathogenesis of commonly ... Bogaert L, Van Poucke M, De Baere C, Dewulf J, Peelman L, Ducatelle R, Gasthuys F, Martens A: Bovine papillomavirus load and ... Equine sarcoids: Bovine Papillomavirus type 1 transformed fibroblasts are sensitive to cisplatin and UVB induced apoptosis and ...
Similar papillomaviruses of ungulates (e.g. deer papillomavirus, European elk papillomavirus, ovine papillomavirus 1,2) are ... Campo, MS (2006). "Bovine papillomavirus: old system, new lessons?". In Campo, MS (ed.). Papillomavirus Research: From Natural ... Chambers G, Ellsmore VA, OBrien PM, Reid SW, Love S, Campo MS, Nasir L (2003). "Association of bovine papillomavirus with the ... Bovine papillomaviruses (BPV) are a paraphyletic group of DNA viruses of the subfamily Firstpapillomavirinae of ...
Bovine papillomavirus type 1 (BPV-1) activity is necessary for the transformation phenotype of equine fibroblasts. Among the ... Animals, Binding Sites, Bovine papillomavirus 1, Cell Line, Gene Expression Profiling, Horses, Host-Pathogen Interactions, ... Finally, BPV-1 E5, E6 and E7 proteins increased MMP-1 mRNA and protein expression. In conclusion, the expression of MMP-1 can ... c-Jun and Fra-1, two components of the AP-1 transcription factor complex, were overexpressed and activated by BPV-1 in equine ...
Association with bovine papillomavirus (BPV) type 1, as it occurs in horses, was demonstrated by polymerase chain reaction, ... Sarcoids in captive zebras (Equus burchellii): association with bovine papillomavirus type 1 infection. ... Sarcoids in captive zebras (Equus burchellii): association with bovine papillomavirus type 1 infection.. ... Zebra 1 (Equus burchellii boehmi) was a 4.5-yr-old, captive-born male that presented with a 9- by 7-cm inguinal mass. Seven ...
The three-dimensional structure of bovine papillomavirus has been determined to 9 A resolution by reconstruction of high ... Novel structural features of bovine papillomavirus capsid revealed by a three-dimensional reconstruction to 9 A resolution Nat ... The three-dimensional structure of bovine papillomavirus has been determined to 9 A resolution by reconstruction of high ... B L Trus 1 , R B Roden, H L Greenstone, M Vrhel, J T Schiller, F P Booy ...
... was developed to investigate the effect of bovine papillomavirus E1 protein on host cell growth. Expression of E1 in CE1 cells ... Bovine papillomavirus 1 / genetics, metabolism*. Cattle. Cell Cycle / physiology*. Cell Division. Cell Line, Transformed. DNA- ... A stable E1-expressing cell line, CE1, was developed to investigate the effect of bovine papillomavirus E1 protein on host cell ... 0/DNA-Binding Proteins; 0/E1 protein, Bovine papillomavirus; 0/Recombinant Fusion Proteins; 0/Viral Proteins; 50-02-2/ ...
Bovine papillomavirus type 1 (BPV-1) and less commonly BPV-2 are now widely recognized as the causative agents of the disease. ... Knockdown of BPV-1 oncogene expression by small interfering RNA reversed the transformed phenotype of sarcoid fibroblasts. ... However, it remains unclear to what extent BPV-1 proteins are involved in the transformation of equine cells. To address this ... Together, these observations strongly suggest that BPV-1 proteins play indispensable roles in the transformation of equine ...
... a sarcoid-derived fully transformed cell line harbouring bovine papilloma virus (BPV)-1 genome. Evidence of a possible ... From: BAG3 protects Bovine Papillomavirus type 1-transformed equine fibroblasts against pro-death signals ...
... Lies ... "A Novel Murine Model for Evaluating Bovine Papillomavirus Prophylactics/therapeutics for Equine Sarcoid-like Tumours." Journal ... "A Novel Murine Model for Evaluating Bovine Papillomavirus Prophylactics/therapeutics for Equine Sarcoid-like Tumours." JOURNAL ... Equine sarcoids are highly recurrent bovine papillomavirus (BPV)-induced fibroblastic neoplasms that are the most common skin ...
Fingerprint Dive into the research topics of Mutational analysis of transcriptional activation by the bovine papillomavirus ... Mutational analysis of transcriptional activation by the bovine papillomavirus type 1 E6. ...
There is still controversy in the scientific field about whether certain types of cutaneous human papillomaviruses (HPVs) are ... There is still controversy in the scientific field about whether certain types of cutaneous human papillomaviruses (HPVs) are ... to provide reasonable arguments for public health institutions to classify at least certain cutaneous HPVs as group 1 ... to provide reasonable arguments for public health institutions to classify at least certain cutaneous HPVs as group 1 ...
bovine papillomavirus type 1 (synthetic). Aethiops sabeus (African Green Monkey). No. SIE. HTML , XML. ... human papillomavirus (HPV-16). Homo sapiens. Yes. UE. HTML , XML. 147. E2 (16 E2-dm). Wang 2003. human papillomavirus (HPV-16) ... human papillomavirus (HPV-16). Homo sapiens. No. SIE. HTML , XML. 149. L2h. Leder 2001. human papillomavirus (HPV-16). Homo ... Human papillomavirus (HPV). Solanum tuberosum. No. UE. HTML , XML. 268. L1s. Warzecha 2003. Human papillomavirus (HPV). Solanum ...
Bovine immunodeficiency virus. Homo sapiens. No. SIE. HTML , XML. 40. L1 (HB PV L1). Gu 2004. Bovine papillomavirus type 1. ... human papillomavirus (HPV-16). Homo sapiens. Yes. SIE. HTML , XML. 187. E7. Kim MS 2005. human papillomavirus (HPV-16). Mus ... human papillomavirus (HPV-16). Homo sapiens. Yes. IE. HTML , XML. 182. E1 (16 E1-dm). Wang 2003. human papillomavirus (HPV-16) ... Human papillomavirus. Homo sapiens. Yes. SIE. HTML , XML. 174. E7h. Cheung 2004. Human papillomavirus. Mus musculus. Unknown. ...
An existing bovine papilloma virus-based expression vector, pBV-1MTHA, was modified to allow transformed X63Ag8-653 myeloma ... An existing bovine papilloma virus-based expression vector, pBV-1MTHA, was modified to allow transformed X63Ag8-653 myeloma ...
BAG3 down-modulation in EqSO4b, a sarcoid-derived fully transformed cell line harbouring bovine papilloma virus (BPV)-1 genome ... BAG3 down-modulation in EqSO4b, a sarcoid-derived fully transformed cell line harbouring bovine papilloma virus (BPV)-1 genome ... BAG3 protects Bovine Papillomavirus type 1-transformed equine fibroblasts against pro-death signals. ... BAG3 protects Bovine Papillomavirus type 1-transformed equine fibroblasts against pro-death signals ...
Intranuclear inclusions in renal tubular epithelium in immunodeficient mice stain with antibodies for bovine papillomavirus ... Immunohistochemistry using polyclonal anti-bovine papillomavirus type 1 (BPV-1) L1 antibody, Camvir monoclonal anti- ... Intranuclear inclusions in renal tubular epithelium in immunodeficient mice stain with antibodies for bovine papillomavirus ... papillomavirus antibody (directed against the seven amino acids GFGAMDF found in human papillomavirus (HPV) 16 L1 protein), a ...
Tammur, J., Sibul, H., Ustav, E. et al. Erratum to: A bovine papillomavirus-1 based vector restores the function of the low- ... Erratum to: A bovine papillomavirus-1 based vector restores the function of the low-density lipoprotein receptor in the ... Tammur J, Sibul H, Ustav E, Ustav M, Metspalu A: A bovine papillomavirus-1 based vector restores the function of the low- ... In the original article [1], the source of the ldlA7 cell line was not stated. This cell line was generously provided by Dr. ...
Fingerprint Dive into the research topics of Interaction with CBP/p300 enables the bovine papillomavirus type 1 E6 oncoprotein ... The E6 oncoprotein of bovine papillomavirus type 1 (BPV-1) can transform cells independently of p53 degradation. The precise ... N2 - The E6 oncoprotein of bovine papillomavirus type 1 (BPV-1) can transform cells independently of p53 degradation. The ... AB - The E6 oncoprotein of bovine papillomavirus type 1 (BPV-1) can transform cells independently of p53 degradation. The ...
Bovine papillomavirus type-1 (BPV-1)-derived vectors could be suitable for gene therapy due to their episomal maintenance at ... The constructed BPV-1 based vector p3.7LDL produced functionally active LDL receptors in the LDLR-deficient cell line CHO-ldlA7 ... We constructed the BPV-1 based vector harbouring the human low-density lipoprotein receptor (LDLR) gene cDNA and tested its ... The rationale of using bovine papillomavirus-1 (BPV-1) derived vectors in gene therapy protocols lies in their episomal ...
From Human papillomavirus type 16.. E6. P06931. 3. EBI-8516738,EBI-7281937. From Bovine papillomavirus type 1. ... "Interaction of oncogenic papillomavirus E6 proteins with fibulin-1.". Du M., Fan X., Hong E., Chen J.J.. Biochem. Biophys. Res ... Microbial infection) Interacts with human papillomavirus/HPV type 16, 18 and 31 proteins E6.1 Publication. ,p>Manually curated ... Cited for: INTERACTION WITH HIGH-RISK HUMAN PAPILLOMAVIRUSES E6 PROTEIN (MICROBIAL INFECTION), INHIBITION OF E6-MEDIATED ...
Keywords: papillomavirus, peripheral blood lymphocytes, tumor cell cultures, chromosome aberrations, genomic instability ... Chromosome Aberrations as a Biomarker for Genomic Instability in Cell Cultures Originated From Bovines, Canines and Equines ... Chromosomal abnormalities or aberrations were investigated in ten bovines, three canines and three equines presenting cutaneous ... Canines and Equines Infected With Papillomavirus. International Journal of Applied Sciences and Biotechnology, 4(1), 104-112. ...
Bovine papilloma virus (BPV-1);. Epstein-Barr virus (phEBO; pREP- derived, and p205);. ... fetal bovine serum (FBS, Gibco-BRL), 2 mM glutamine, 100 U/ml penicillin and 100 μg/ml streptomycin at 37 C. in 5% CO2. Full ... 1: 27-31 (1995); Kyriakides et al., J. Cell Biol. 140: 419-430 (1998)]; and integrins αvβ5 and αvβ3 [Brooks et al., Science 264 ... Overexpression of syndecan-1 in 3T3 cells led to inhibition of bFGF-induced growth [Mali et al., J. Biol. Chem. 268: 24215- ...
Differential expression of microRNAs in bovine papillomavirus type 1 transformed equine cells. ... The impact of tick-borne pathogen infection in Indian bovines is determined by host type but not the genotype of Theileria ... Characterization of HSP90 isoforms in transformed bovine leukocytes infected with Theileria annulata. ... 2018 Nov 1;144:274-284. doi: 10.1016/j.watres.2018.07.039. Epub 2018 Jul 17. ...
  • To investigate the efficiency of encapsidation of plasmid by papillomavirus virus-like particles (PV VLPs), and the infectivity of the resultant PV pseudovirions, Cos-1 cells were transfected with an 8-kb plasmid incorporating a green fluorescent protein (GFP) reporter gene (pGSV), and infected with bovine PV (BPV-1) L1/L2 recombinant vaccinia virus to produce BPV1 pseudovirions. (springer.com)
  • This antibody reacts with BPV type 1 E2 protein and has been mapped to amino acids 310-410. (thermofisher.com)
  • The bovine papillomavirus type 1 E6 (BE6) binds to LXXLL peptide sequences termed LD motifs (consensus sequence LDXLLXXL) on the cellular protein paxillin that is a substrate of Src and focal adhesion kinases. (asm.org)
  • Recombinant protein corresponding to full length Bovine Papillomavirus type-1 transactivator protein E2. (abnova.com)
  • Bovine Papillomavirus type 1 E2-protein. (abnova.com)
  • Spastin, the most commonly mutated protein in hereditary spastic paraplegia interacts with Reticulon 1 an endoplasmic reticulum protein. (abnova.com)
  • The papillomavirus E2 protein is required for viral transcriptional regulation, DNA replication and genome segregation. (asm.org)
  • The X-ray crystal structure of the transactivation domain of the human papillomavirus type 16 (HPV16) E2 protein was recently determined and was found to consist of an cashew-shaped domain made up of two regions ( 4 ). (asm.org)
  • Upregulation of equine matrix metalloproteinase 1 by bovine papillomavirus type 1 is through the transcription factor activator protein-1. (ucl.ac.uk)
  • A putative activator protein-1 (AP-1)-binding site was demonstrated to be crucial for upregulated MMP-1 promoter activity by BPV-1. (ucl.ac.uk)
  • Finally, BPV-1 E5, E6 and E7 proteins increased MMP-1 mRNA and protein expression. (ucl.ac.uk)
  • The bovine papillomavirus E1 protein alters the host cell cycle and growth properties. (biomedsearch.com)
  • A stable E1-expressing cell line, CE1, was developed to investigate the effect of bovine papillomavirus E1 protein on host cell growth. (biomedsearch.com)
  • The E7 protein of cutaneous human papillomavirus type 8 causes invasion of human keratinocytes into the dermis in organotypic cultures of skin. (microbiologyresearch.org)
  • The comparison of the CBP/p300-binding properties of BPV-1 E6 mutants previously characterized in transcription and transformation studies suggests (i) that the E6-CBP/p300 interaction may be necessary, but not sufficient, for cell transformation, and (ii) that the transcriptional activator function, inherent to the E6 protein, is not derived from forming a complex with CBP/p300. (elsevier.com)
  • In order to facilitate the study of potential equine sarcoid prophylactics or therapeutics, which can be a slow and costly process in equines, a murine model for BPV-1 protein-expressing equine sarcoid-like tumours was developed in mice through stable transfection of BPV-1 E5 and E6 in a murine fibroblast tumour cell line (K-BALB). (ugent.be)
  • The E1 protein of bovine papilloma virus 1 is an ATP-dependent DNA helicase. (springer.com)
  • TNV/p28 tev , p18 6Drev , Tat1-Rev2, Tat^8c, p17 tev , or Ref) are the result of alternative splicing events, Tat-T/Vpt is produced upon programmed ribosomal frameshifting, and a Rev1-Vpu fusion protein is expressed due to a nucleotide polymorphism that is unique to certain HIV-1 clade A and C strains. (frontiersin.org)
  • The paragon of such a multitasking or moonlighting protein is HIV-1 Nef, which has been described to downmodulate a variety of surface receptors including CD4, MHC class I, CD28, and CXCR4, counteracts the host restriction factors SERINC3/5, and upregulates the invariant chain/CD74 to suppress antigen presentation ( Pereira and daSilva, 2016 ). (frontiersin.org)
  • Interacts with bovine papillomavirus type 1 regulatory protein E2 and human herpes virus-8 (HHV-8) protein LANA. (rcsb.org)
  • In this study, early (E) and late (L) protein expression of bovine papillomavirus type 2 (BPV-2) in the urothelium of the urinary bladder is described in cows and water buffaloes suffering from naturally occurring papillomavirus-associated urothelial bladder tumors. (nih.gov)
  • The major capsid protein, L1, believed to be only expressed in productive papillomavirus infection was detected by Western blot analysis. (nih.gov)
  • The CAMVIR-1 antibody was raised against the major capsid protein L1 of human papillomavirus type 16, using a recombinant vaccinia virus that expresses the L1 protein. (abcam.com)
  • Naturally-occurring variants of Human papillomavirus (HPV) 58 have been defined as lineages and sublineages but little is known about the impact of this diversity on protein function. (bioportfolio.com)
  • The L1 capsid protein of papillomaviruses ( 45 ) expressed in yeast ( 15 , 36 ), insect cells ( 14 , 18 ), or mammalian cells ( 45 ) self assembles into virus-like particles (VLPs) that are composed of 72 pentamers of L1 in a T=7 icosahedral structure ( 1 ). (asm.org)
  • AU1 is a peptide tag with the amino acid sequence DTYRYI, which is derived from the bovine papillomavirus-1 (BPV-1) major capsid protein. (biolegend.com)
  • The bovine papilloma virus E2 protein is the master regulator of papillomavirus replication and transcription. (abcam.cn)
  • 9 different monoclonal antibodies to BPV type 1 E2 protein. (icosagen.com)
  • Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication, J Virol. (icosagen.com)
  • Characterization of the functional activities of the bovine papillomavirus type 1 E2 protein single-chain heterodimers, J Virol. (icosagen.com)
  • Chang OK, Lee BO, Humbert G, Saulnier F. Nutritional advantage of protein fraction in equine milk as compared to bovine milk. (cavalor.com)
  • It has been shown that transforming proteins E5, E6 and E7 are not required whereas E1 and E2 are essential and sufficient for BPV-1 replication [ 14 ]. (biomedcentral.com)
  • 15 ] have demonstrated that a BPV-1-based expression vector B45-Neo containing only E1, E2, a replication origin and an upstream regulatory region (URR). (biomedcentral.com)
  • Crystal structures of two intermediates in the assembly of the papillomavirus replication initiation complex. (cathdb.info)
  • A better understanding of the expression and activity of these non-canonical viral proteins will help to dissect their potential role in viral replication and reveal how HIV-1 optimized the coding potential of its genes. (frontiersin.org)
  • Several antivirals have been identified with the ability to inhibit HSV-1 replication in human cells to a greater or lesser degree, most of which are nucleoside analogues that unfortunately exhibit teratogenic potential, embryotoxicity, carcinogenic or antip. (medworm.com)
  • Individual papillomavirus types tend to be highly adapted to replication in a single animal species. (wikidoc.org)
  • This work also confirms the close proximity of a replication origin and a promoter, with both functions being located within the 1-kb proximal region of the ardC actin gene. (asm.org)
  • Finally, replication of the target DNA is a prerequisite for efficient demethylation since only plasmids that carry the bovine papilloma virus sequences which support plasmid replication after the midblastula transition are demethylated. (deepdyve.com)
  • Random-Choice Replication of Extrachromosomal Bovine Papillomavirus (BPV) Molecules in Heterogeneous, Clonally Derived BPV-Infected Cell Lines," J. Virol. (patentgenius.com)
  • The replication of papillomavirus genomes in yeast is not limited to HPV16. (unl.edu)
  • The episomal replication of papillomavirus genomes in yeast provides a genetically manipulatable system in which to investigate cellular factors required for episomal replication and may provide a novel means for generating infectious papillomavirus. (unl.edu)
  • K. N. Zhao and I. H. Frazer, " Saccharomyces cerevisiae is permissive for replication of bovine papillomavirus type 1," Journal of Virology , vol. 76, no. 23, pp. 12265-12273, 2002. (hindawi.com)
  • The major focus of my laboratory is in understanding the molecular machines that make up the DNA replication forks of the small human DNA viruses, polyoma- and papillomaviruses. (buffalo.edu)
  • Sarcoids in captive zebras (Equus burchellii): association with bovine papillomavirus type 1 infection. (oregonstate.edu)
  • The impact of tick-borne pathogen infection in Indian bovines is determined by host type but not the genotype of Theileria annulata. (nih.gov)
  • Many studies have suggested a possible link between breast cancer pathogenesis and viral infection, particularly mouse mammary tumour virus, simian virus 40, Epstein-Barr virus, and human papillomavirus (HPV). (springer.com)
  • Publication date: Available online 25 May 2019Source: Antiviral ResearchAuthor(s): B. Praena, R. Bello-Morales, F. de Castro, J.A. López-GuerreroAbstractHerpes simplex virus type 1 (HSV-1) is a ubiquitous infectious agent that can establish latency in neurons, and in some cases, viral retrograde transport results in infection of the central nervous system (CNS). (medworm.com)
  • Productive infection of bovine papillomavirus type 2 in the urothelial cells of naturally occurring urinary bladder tumors in cattle and water buffaloes. (nih.gov)
  • Clinical aspects of HPV infection are outlined in the main article human papillomavirus . (wikidoc.org)
  • [3] Most papillomavirus types are adapted to infection of particular body surfaces. (wikidoc.org)
  • However, the authors note that the chimpanzee-specific papillomavirus sequence could have been the result of surface contamination of the zookeeper's skin, as opposed to productive infection. (wikidoc.org)
  • BPV-1 infection of horses, which are an incidental host for the virus, can lead to the development of benign tumors known as sarcoids . (wikidoc.org)
  • Persistent infection by bovine papillomavirus. (microbiologyresearch.org)
  • Latent papillomavirus infection in cattle. (microbiologyresearch.org)
  • Human papillomavirus infection is associated with the development of malignant and benign neoplasms. (bioportfolio.com)
  • The purpose of this study is to determine the prevalence and type distribution of genital human papillomavirus (HPV) infection in Tanzanian men. (bioportfolio.com)
  • Fewer topics in obstetrics and virology will generate stronger opinions backed only by anecdotal data than the issue of human papillomavirus (HPV) infection during pregnancy. (springer.com)
  • Few consensus opinions among these diverse disciplines exist, although most would agree that (1) HPV genital infections in women (and men) have increased dramatically in the past two decades, (2) our ability to detect smaller amounts of HPV DNA continues to improve, and (3) laryngeal papillomatosis (by far the worst neonatal "outcome" of maternal HPV infection) is an uncommon but potentially devastating problem best treated by prevention, if possible. (springer.com)
  • Prevalence of papillomavirus infection in col- poscopically directed cervical biopsy specimens in 1972 and 1982. (springer.com)
  • Genital human papillomavirus infection. (springer.com)
  • The major factor contributing to the development of cervical cancer is persistent infection with high-risk human papillomaviruses (HPVs) [ 1 , 2 ]. (medsci.org)
  • Several studies have shown that immunization with VLPs induces neutralizing antibodies and protects against experimental papillomavirus infection in rabbits ( 5 ), dogs ( 39 ), and cows ( 17 ). (asm.org)
  • Human papillomavirus is one of the most common causes of sexually transmitted infection in the world and can also cause cancer. (caister.com)
  • Infectivity studies of transgenic Arabidopsis showed that overexpression of CYCD3;1 or E2FB, both of which promote the mitotic cell cycle, strongly impaired CaLCuV infection. (plantphysiol.org)
  • The epidemiology of cervical cancer indicates the presence of a sexually transmitted risk factor, attributable at least in part to infection with human papillomavirus (HPV) type 16 or 18. (aacrjournals.org)
  • Papillomavirus E6 proteins are adapters that change the function of cellular regulatory proteins. (asm.org)
  • It localizes to focal adhesions and associates with focal adhesion proteins implicated in the regulation of cell attachment, spreading, and migration, including focal adhesion kinase (FAK), GIT1, PAK, Src, and Crk (reviewed in reference 6 and illustrated in Fig. 1 ). (asm.org)
  • However, it is not yet known how BPV-1 proteins regulate equine MMP-1 expression. (ucl.ac.uk)
  • BPV-1 E6 and E7 proteins increased MMP-1 promoter activity, and inhibition of BPV-1 gene expression by small interfering RNA significantly reduced the promoter activity. (ucl.ac.uk)
  • However, it remains unclear to what extent BPV-1 proteins are involved in the transformation of equine cells. (microbiologyresearch.org)
  • Together, these observations strongly suggest that BPV-1 proteins play indispensable roles in the transformation of equine fibroblasts. (microbiologyresearch.org)
  • These data also suggest that BPV-1 proteins are potential drug targets for equine sarcoid therapy. (microbiologyresearch.org)
  • Cooperative transformation and coexpression of bovine papillomavirus type 1 E5 and E7 proteins. (microbiologyresearch.org)
  • Here it is shown that BPV-1 E6 interacts with CBP/p300 in the same way as described for the E6 proteins of oncogenic human papillomaviruses. (elsevier.com)
  • Like equine sarcoids, these murine tumour cells (BPV-KB) were of fibroblast origin, were tumorigenic and expressed BPV-1 proteins. (ugent.be)
  • DiMaio D, Mattoon D. Mechanisms of cell transformation by papillomavirus E5 proteins. (springer.com)
  • In total, HIV-1 expresses 16 canonical proteins from only nine genes within its 10 kb genome. (frontiersin.org)
  • In addition to the canonically expressed proteins, a growing number of publications describe the existence of non-canonical fusion proteins in HIV-1 infected cells. (frontiersin.org)
  • The goal of this review is to provide an overview of previously described HIV-1 fusion proteins and to summarize our current knowledge of their expression patterns and putative functions. (frontiersin.org)
  • In addition, fusion proteins and DNA conjugates which contain a type 1 interferon/CD40 agonist/antigen. (google.com.au)
  • In addition, fusion proteins and DNA conjugates which contain a type 1 interferon/CD40 agonist/antigen combination are provided. (google.com.au)
  • ABSTRACT Papillomavirus E6 oncoproteins transform mammalian cells through interaction with cellular proteins. (semanticscholar.org)
  • The papillomavirus E2 proteins bind to Brd4, and some utilize this interaction in tethering the viral genomes to mitotic chromosomes ( 1 , 3 , 57 , 59 ). (asm.org)
  • Impact of Naturally Occurring Variation in the Human Papillomavirus (HPV) 58 Capsid Proteins on Recognition by Type-Specific Neutralizing Antibodies. (bioportfolio.com)
  • The present invention relates to polypeptides that immunologically mimic papillomavirus latent proteins and to antibodies and monoclonal antibodies that immunoreact with papillomavirus latent proteins. (freepatentsonline.com)
  • The transactivation domain contains many residues that are invariant among all papillomavirus E2 sequences examined. (asm.org)
  • The transactivation domain contains many residues that are invariant among all papillomavirus E2 sequences examined thus far. (asm.org)
  • Insertion of DNA sequences at a unique restriction enzyme site engineered for vector purposes into the genome of herpes simplex virus type 1, pp. 2931-2939. (patentgenius.com)
  • [7] Although a wide variety of papillomavirus sequences were identified in the study, the authors found little evidence for inter-species transmission. (wikidoc.org)
  • Genomic sequences of bovine papillomaviruses in formalin-fixed sarcoids from Australian horses revealed by polymerase chain reaction. (microbiologyresearch.org)
  • Four novel papillomavirus sequences support a broad diversity among equine papillomaviruses. (uzh.ch)
  • Identification of two novel equine papillomavirus sequences suggests three genera in one cluster. (uzh.ch)
  • N.Bloch N.BlochNatalieN.Bloch N.BlochNataliehttps://api.elsevier.com/content/author/author_id/6701420212 (1994) Genomic sequences of bovine papillomaviruses in formalin-fixed sarcoids from Australian horses revealed by polymerase chain reaction. (edu.au)
  • Seventy six formalin-fixed paraffin-embedded sarcoids from 62 Australian horses, collected over a ten year period, were examined for the presence of genomic sequences from bovine papillomavirus 1 and 2 (BPV1, BPV2) with the polymerase chain reaction (PCR). (edu.au)
  • The specificity of the types differs: BPV-1 infects paragenital areas, including penis, teats and udders BPV-2 infects skin, alimentary canal and urinary bladder Xipapillomavirus or epitheliotropic BPVs (formerly known as subgroup B), including types 3, 4 and 6, have a smaller genome of around 7.3 kb and are unique among papillomaviruses in lacking the E6 oncoprotein. (wikipedia.org)
  • Presence of bovine papillomavirus type 2 DNA and expression of the viral oncoprotein E5 in naturally occurring urinary bladder tumours in cows. (microbiologyresearch.org)
  • Bovine papillomavirus E5 oncoprotein binds to the activated form of the platelet-derived growth factor β receptor in naturally occurring bovine urinary bladder tumours. (microbiologyresearch.org)
  • The E6 oncoprotein of bovine papillomavirus type 1 (BPV-1) can transform cells independently of p53 degradation. (elsevier.com)
  • In all tumor cases, the BPV-2 E5 oncoprotein was detected by immunoprecipitation (Figure 1). (nih.gov)
  • In bovine papillomavirus type 1 (BPV1), the E2 gene encodes three different polypeptides ( 22 ). (asm.org)
  • Two horses demonstrated multiple lesions: the sarcoids of one of these contained only BPV1 DNA and those of the other contained three types of bovine Deltapapillomavirus (BPV1, 2, and 13). (scielo.br)
  • Journal of Virology, 83(1):408-419. (uzh.ch)
  • Genomic comparison of bovine papillomavirus 1 isolates from bovine, equine and asinine lesional tissue samples. (uzh.ch)
  • Entire genomic sequence of novel canine papillomavirus type 13. (uzh.ch)
  • 4. The immunostimulatory regimen of claim 1 wherein the an agonistic CD40L polypeptide or CD40 agonistic antibody or antibody fragment, type 1 interferon and viral antigen are administered as discrete polypeptides. (google.com.au)
  • 8. The immunostimulatory regimen of claim 2 wherein the an agonistic CD40L polypeptide or CD40 agonistic antibody or antibody fragment, type 1 interferon and viral antigen are administered as discrete compounds in combination. (google.com.au)
  • Papillomaviruses normally replicate in stratified squamous epithelial tissues of their mammalian hosts, in which the viral genome is found as a nuclear plasmid. (unl.edu)
  • In conclusion, the expression of MMP-1 can be enhanced by BPV-1 oncoproteins E6 and E7 through the AP-1 transcription factor and by E5 via an indirect mechanism. (ucl.ac.uk)
  • These findings shed light on the mechanism of BPV-1-mediated equine fibroblast infiltration and indicate that both BPV-1 oncoproteins and AP-1 could be potential targets for equine sarcoid therapy. (ucl.ac.uk)
  • Expression of platelet-derived growth factor- β receptor and bovine papillomavirus E5 and E7 oncoproteins in equine sarcoid. (microbiologyresearch.org)
  • Papillomavirus research has been revolutionised in recent years with the advent of new technologies such as organotypic raft cultures, virus-like particles and transgenic mice. (caister.com)
  • Figure 1 (left): Transmission Electron Microscopy image of empty non-infectious Bovine papillomavirus virus-like particles produced at the James Hutton Institute using plant expression systems. (hutton.ac.uk)
  • Danos O, Engel LW, Chen EY, Yaniv M, Howley PM. Comparative analysis of the human type la and bovine type 1 papilloma-virus genomes. (springer.com)
  • As observed by Robbins et al 1 and Plotkin, 2 almost all current vaccines work by the induction of antibodies in serum or on the mucosa (by local production or transudation from serum) to block adherence of pathogens to epithelial cells or interfere with microbial invasion of the bloodstream. (aappublications.org)
  • Detection of antibodies against epidermodysplasia verruciformis-associated canine Papillomavirus 3 in sera of dogs from Europe and Africa by enzyme-linked immunosorbent assay. (uzh.ch)
  • We describe here a new multiplexed assay, by using the Luminex Laboratory MultiAnalyte Profiling (LabMAP3) assay system, that can simultaneously quantitate neutralizing antibodies to human papillomavirus types 6, 11, 16, and 18 in 50 μl of serum. (asm.org)
  • IgG and IgA antibodies were measured against a panel of 12 antigens derived from HPV types 6, 11,1 6, or 18, as well as against Herpes simplex virus type 1 and 2, Chlamydia trachomatis , cytomegalovirus, Epstein-Barr virus, and bovine papillomavirus. (aacrjournals.org)
  • Antibodies against cytomegalovirus, Herpes simplex virus type 2, Epstein-Barr virus, or bovine papillomavirus were, on their own, not significantly associated with cervical cancer, but seropositivity against multiple infections was associated with a successively increased relative risk. (aacrjournals.org)
  • BPVs have been used as a model for studying papillomavirus molecular biology and for dissecting the mechanisms by which this group of viruses cause cancer. (wikipedia.org)
  • Molecular mechanisms of transformation by the human papillomaviruses. (springer.com)
  • The research identified papillomavirus oncogenes and the molecular mechanisms of their damaging effects. (wikipedia.org)
  • This work is considered fundamental for the understanding of the pathogenesis of papillomaviruses at the molecular level. (wikipedia.org)
  • In this timely book leading scientists review current aspects of papillomavirus research providing a fascinating insight into papillomavirus molecular biology, interactions with the host, immunology and vaccine development. (caister.com)
  • A novel short peptide is a specific inhibitor of the human immunodeficiency virus type 1 integrase," Journal of Molecular Biology , vol. 318, no. 1, pp. 45-58, 2002. (hindawi.com)
  • The following antibody was used in this experiment: Bovine Papilloma Virus Type 1 E2 Monoclonal Antibody (3E8) from Thermo Fisher Scientific, catalog # MA1-71536, RRID AB_962047. (thermofisher.com)
  • Mouse monoclonal antibody raised against Bovine papillomavirus type 1 E2. (abnova.com)
  • Specific levels of antibody have been correlated with protection against many diseases (Table 1 ). (aappublications.org)
  • 22. The immunostimulatory regimen of claim 1 wherein the CD40 agonistic antibody or fragment is an agonistic chimeric, human, or humanized anti-CD40 antibody or an agonistic CD40 antibody fragment which is selected from a Fab, F(ab′) 2 , Fd, and a Fv. (google.com.au)
  • The broad spectrum HPV antibody was produced (1H8) against SDS-disrupted bovine papillomavirus type 1 (BPV-1) and used to identify the product of the L1 open reading frame (ORF) of BPV-1. (abcam.com)
  • The antibody was tested with ELISA and with an immunofluorescent technique and detected HPV-1, 6, 11, 16, 18, and 31 in formalin-fixed paraffin embedded biopsy specimens. (abcam.com)
  • 15. A purified antibody, capable of selectively binding to a CPU mutant polypeptide according to claim 1. (freepatentsonline.com)
  • Several different methods have been developed to quantitate neutralizing antibody responses to human papillomaviruses (HPVs), including in vivo neutralization assays, in vitro pseudoneutralization assays, competitive radioimmunoassays (cRIAs), and enzyme-linked immunosorbent assays. (asm.org)
  • Antibody concentration of 1 µg/ml was used. (icosagen.com)
  • In vivo experiments should reveal, whether 1-5% transfection efficiency obtained in the current work is sufficient to bring about detectable and clinically significant lowering of the amount of circulating LDL cholesterol particles. (biomedcentral.com)
  • As an initial investigation of the preclinical potential of this tumour model for equine sarcoids prophylactics, mice were immunized with BPV-1 E5E6 Venezuelan equine encephalitis virus replicon particles, prior to BPV-KB challenge, which resulted in an increased tumour-free period compared with controls, indicating that the BPV-KB murine model may be a valuable preclinical alternative to equine clinical trials. (ugent.be)
  • human papillomavirus (HPV) types in cervical cancer are HPV16 and HPV18. (bioportfolio.com)
  • Human papillomavirus type 16 (HPV16) is a high-risk HPV type and a potent carcinogen. (medsci.org)
  • In this study, we describe experiments investigating the capacity of the human papillomavirus type 16 (HPV16) genome to replicate in yeast ( Saccharomyces cerevisiae ). (unl.edu)
  • Laboratory tests were used to detect nucleic acid of papillomaviruses, polyomaviruses, circoviruses and anelloviruses (4/6 mice), a specific PCR was used to detect murine polyomavirus (1/6), and a panel of serological tests was used to detect seroconversion to major murine pathogens (1/6). (edu.au)
  • Apart from human papillomaviruses, the laboratory team focuses on human polyomaviruses and other small DNA viruses (anelloviruses or human bocavirus). (cuni.cz)
  • human papillomavirus (HPV) results in a vast number of human cancers, and the human polyomaviruses JC and BK cause serious disease and death in immunocompromised patients. (buffalo.edu)
  • RATIONALE: Vaccines made from human papillomavirus may help the body build an effective immune response to kill HIV cells. (bioportfolio.com)
  • Different livestock vaccines protect against bovine papilloma virus, foot rot, liver abscesses, bovine pinkeye and blackleg and many others. (allivet.com)
  • As with rabbit papillomaviruses, some HPV types can cause cancer, most notably cervical cancer . (wikidoc.org)
  • This randomized phase III trial studies how well human papillomavirus (HPV) vaccine therapy works in reducing high-grade cervical lesions in patients with human immunodeficiency virus (HIV. (bioportfolio.com)
  • Analysis of individual human papillomavirus types in cervical neoplasia: A possible role for type 18 in rapid progression. (springer.com)
  • Increased frequency of detection of human papillomavirus DNA in exfoliated cervical cells during pregnancy. (springer.com)
  • In situ hybridization detection of human papillomavirus DNAs and messenger RNAs in genital condylomas and a cervical carcinoma. (springer.com)
  • Yao Y, Yan Z, Dai S, Li C, Yang L, Liu S, Zhang X, Shi L, Yao Y. Human Papillomavirus Type 16 E1 Mutations Associated with Cervical Cancer in a Han Chinese Population. (medsci.org)
  • Human papillomaviruses (HPVs) are double-stranded DNA viruses that infect epithelial cells and are significantly associated with low-grade cervical intraepithelial neoplasia, genital condyloma, and cervical cancer ( 23 ). (asm.org)
  • The link between human papillomaviruses and human cervical cancers has long been established. (pubmedcentralcanada.ca)
  • HPVs were first associated with cervical cancer due to the detection of HPV DNA in tumor biopsies [ 1 ]. (pubmedcentralcanada.ca)
  • Bovine papillomavirus type 1 infects not only cattle but also equids and is a causative factor in the pathogenesis of commonly occurring equine sarcoid tumours. (biomedcentral.com)
  • Bovine papillomaviruses (BPV) are a paraphyletic group of DNA viruses of the subfamily Firstpapillomavirinae of Papillomaviridae that are common in cattle. (wikipedia.org)
  • Infectious cattle cutaneous papillomatosis or warts are commonly regarded as either hyperplasia or benign neoplastic conditions caused by bovine papilloma virus (Boiron et al. (thefreelibrary.com)
  • Table 1 and Table 2 report histological diagnosis of urothelial tumors of the urinary bladder of cattle and buffaloes, respectively, performed according to recent morphological criteria [22]. (nih.gov)
  • [9] In its natural host (cattle), BPV-1 induces large fibrous skin warts. (wikidoc.org)
  • Bovine papillomavirus (BPV) induces exophytic papillomas or 'warts'and fibropapillomas in cattle 3 and has been associated with malignant transformation to carcinoma of the urinary bladder and upper alimentary tract in cattle experimentally fed bracken fern ( Pteridium aquilinum ) 28 . (scielo.org.za)
  • Papillomaviruses are a diverse group of DNA-based viruses that infect the skin and mucous membranes of humans and a variety of animals. (thermofisher.com)
  • It is now recognized that papillomaviruses are a diverse group of non- enveloped DNA viruses that infect animals ranging from birds to manatees . (wikidoc.org)
  • Papillomaviruses are oncogenic DNA tumour viruses that infect humans and animals. (caister.com)
  • Equine sarcoids are highly recurrent bovine papillomavirus (BPV)-induced fibroblastic neoplasms that are the most common skin tumours in horses. (ugent.be)
  • The bovine papillomavirus (BPV) types 1 and 2 may be involved in the pathogenesis of sarcoids, and probably the recently described BPV type (BPV13) might be associated with the pathogenesis of this lesion. (scielo.br)
  • Detection of bovine papillomavirus DNA on the normal skin and in the habitual surroundings of horses with and without equine sarcoids. (microbiologyresearch.org)
  • Bovine papillomavirus DNA in neoplastic and nonneoplastic tissues obtained from horses with and without sarcoids in the western United States. (microbiologyresearch.org)
  • Bovine papillomavirus (BPV), primarily types 1 and 2, is now considered the main etiologic agent of equine sarcoids. (merckvetmanual.com)
  • Glycoprotein D of bovine herpesvirus 5 (BoHV-5) confers an extended host range to BoHV-1 but does not contribute to invasion of the brain. (uzh.ch)
  • Cloning of Bovine herpesvirus type 1 and type 5 as infectious bacterial artifical chromosomes. (uzh.ch)
  • There is still controversy in the scientific field about whether certain types of cutaneous human papillomaviruses (HPVs) are causally involved in the development of non-melanoma skin cancer (NMSC). (frontiersin.org)
  • Clear scientific evidence about the etiology and underlying mechanisms involved in NMSC development is fundamental to provide reasonable arguments for public health institutions to classify at least certain cutaneous HPVs as group 1 carcinogens. (frontiersin.org)
  • However, human papillomaviruses (HPVs) are now being detected in another type of cancer, not previously associated with this virus, head and neck squamous cell carcinoma (HNSCC). (pubmedcentralcanada.ca)
  • One major advantage of HIV-1 and related retroviruses compared to their host species is certainly their high mutation rate that allows them to quickly adapt to an ever-changing environment. (frontiersin.org)
  • Howley and his co-workers created gene maps of many species of papillomaviruses and analyzed their transcription patterns and systems of transcription regulation. (wikipedia.org)
  • Inter-species transmission has also been documented for bovine papillomavirus (BPV) type 1. (wikidoc.org)
  • Transmissible spongiform encephalopathies (TSEs) or prion diseases are invariably lethal neurodegenerative diseases afflicting a wide variety of species, including humans [ 1 ]. (intechopen.com)
  • BPV-1 has recently been detected in several common fly species (eg, house fly and stable fly), and because there is an apparent predilection for sarcoid development at wound sites, it has been proposed that flies may act as vectors as they move between wound sites on different horses. (merckvetmanual.com)
  • Skin lesions associated with papillomaviruses have been reported in many animal species and man. (scielo.org.za)
  • Bovine papillomavirus (BVP) affects mainly the epidermis, but also the dermis in several species including bovine, the best-known example being equine sarcoid, which is associated with BVP types 1 and 2. (scielo.org.za)
  • In this study we show that in equine fibroblasts, BPV-1 sensitises cells to cisplatin-induced and UVB-induced apoptosis, a known cofactor for papillomavirus associated disease, however BPV-1 transformed fibroblasts show increased clonogenic survival, which may potentially limit the therapeutic effects of repeated cisplatin treatment. (biomedcentral.com)
  • Bovine papillomavirus type 1 (BPV-1) activity is necessary for the transformation phenotype of equine fibroblasts. (ucl.ac.uk)
  • Among the many changes induced by BPV-1, matrix metalloproteinase 1 (MMP-1) upregulation contributes to the invasiveness of equine fibroblasts. (ucl.ac.uk)
  • c-Jun and Fra-1, two components of the AP-1 transcription factor complex, were overexpressed and activated by BPV-1 in equine fibroblasts. (ucl.ac.uk)
  • however, unlike other papillomaviruses, they cause proliferation of both keratinocytes and fibroblasts, causing benign fibropapillomas involving both the epithelium and the underlying dermis. (wikipedia.org)
  • Knockdown of BPV-1 oncogene expression by small interfering RNA reversed the transformed phenotype of sarcoid fibroblasts. (microbiologyresearch.org)
  • An existing bovine papilloma virus-based expression vector, pBV-1MTHA, was modified to allow transformed X63Ag8-653 myeloma cells, NIH 3T3 fibroblasts and C127 mammary tumor cells to stably carry multiple copies of the vector, to express the inserted cDNA encoding a single interleukin constitutively, and to secrete the interleukin in high quantities. (nih.gov)
  • Bovine Papilloma Virus Plasmids Replicate Randomly in Mouse Fibroblasts throughout S Phase of the Cell Cycle," Cell 50:59-68 (1987). (patentgenius.com)
  • Giraffe 1 had scattered, and Giraffe 2 numerous, large, anaplastic, at times indistinctly multinucleated dermal fibroblasts with bizarre nuclei within the sarcoid-like lesions, which were BPV-1 positive in Giraffe 1 and BPV-1 and -2 positive in Giraffe 2 by RT-PCR. (scielo.org.za)
  • The 1-kb DNA fragment upstream of the ardC actin gene of Physarum polycephalum promotes the transcription of a reporter gene either in a transient-plasmid assay or as an integrated copy in an ectopic position, defining this region as the transcriptional promoter of the ardC gene ( PardC ). (asm.org)
  • The papillomavirus E2 transcriptional activation functions are also mediated through Brd4 ( 35 , 41 , 42 ). (asm.org)
  • Papillomavirus are small DNA viruses that cause persistent epithelial lesions called papillomas ( 15 ). (asm.org)
  • Template:Taxobox group i entry Template:Taxobox familia entry Template:Taxobox end placement Template:Taxobox section subdivision Template:Taxobox image Template:Taxobox end Papillomaviruses were first identified in the early 20th century, when it was shown that skin warts , or papillomas , could be transmitted between individuals by a filterable infectious agent. (wikidoc.org)
  • EcPV2 DNA in equine papillomas and in situ and invasive squamous cell carcinomas supports papillomavirus etiology. (uzh.ch)
  • Canine inverted papillomas associated with DNA of four different papillomaviruses. (uzh.ch)
  • High prevalence of bovine papillomaviral DNA in the normal skin of equine sarcoid-affected and healthy horses. (microbiologyresearch.org)
  • Schneider A, Hotz M, Gissmann L. Increased prevalence of human papillomaviruses in the lower genital tract of pregnant women. (springer.com)
  • Arranged in three different reading frames, it contains only nine canonical genes (Figure 1 ). (frontiersin.org)
  • O segundo par de primers utilizado é complementar a sequência dos genes E5 e L2 de BPVs 1, 2 e 13. (scielo.br)
  • This study established a PCR protocol allowing detection of ≤10 copies of the BPV-1/-2 genes E5 and L1. (microbiologyresearch.org)
  • this interaction is required for cellular transformation by BE6 ( 1 , 30 ). (asm.org)
  • The development of a bovine papillomavirus (BPV) vaccine is an outstanding challenge. (bireme.br)
  • The agricultural significance of BPV-1 spurred a successful effort to develop a vaccine against the virus. (wikidoc.org)
  • Clinical and Vaccine Immunology : CVI, 16(1):66-72. (uzh.ch)
  • An effective HPV vaccine will most likely require several distinct genotypes to protect against multiple cancer causing papillomaviruses. (asm.org)
  • Essential reading for scientists and researchers working on papillomavirus and a recommended text for anyone involved with antiviral drug and vaccine development. (caister.com)
  • The rationale of using bovine papillomavirus-1 (BPV-1) derived vectors in gene therapy protocols lies in their episomal maintenance at intermediate to high copy number, and stable, high-level expression of the gene products. (biomedcentral.com)
  • We constructed the BPV-1 based vector harbouring the human low-density lipoprotein receptor (LDLR) gene cDNA and tested its ability to restore the function of the LDLR in the receptor-deficient cell line CHO-ldlA7. (biomedcentral.com)
  • Expression of Hepatitis B virus S gene by herpes simplex virus type 1 vectors carrying .alpha. (patentgenius.com)
  • A novel method for converting common restriction enzymes into rare cutters: integration host factor-mediated Achilles' cleavage (IHF-AC), " Gene 110:1-7 (1992. (patentgenius.com)
  • L1 could not be detected in any PBMC DNA, suggesting either deletion or interruption of this gene in PBMCs of BPV-1/-2-infected equines. (microbiologyresearch.org)
  • 2000 Jan 1;81(11):2617-2623. (elsevier.com)
  • For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml (1:1000 - 1:2000 dilution). (biolegend.com)
  • Use of polymerase chain reaction amplification for the detection of genital human papillomaviruses. (springer.com)
  • Two 5 μm sections of formalin-fixed paraffin-embedded (FFPE) tissue samples of 50 neuro-endorcrinal tumor from different entities ( Table 1 ), collected between 1997 and 2008, were obtained from the tissue bank of the National Center for Tumor Diseases (NCT) Heidelberg. (aacrjournals.org)
  • Association with bovine papillomavirus (BPV) type 1, as it occurs in horses, was demonstrated by polymerase chain reaction, nucleic acid sequencing, and in situ hybridization (ISH) on paraffin-embedded tissues from the inguinal mass of zebra 1. (oregonstate.edu)
  • 2 . The method of claim 1 , wherein said nucleic acid molecules of step (a) are double-stranded RNA molecules or encode double-stranded RNA molecules. (google.com)
  • 3 . The method of claim 1 , wherein each nucleic acid molecule to be introduced is contained in a vector. (google.com)
  • 12 . The method of claim 1 , wherein said affixed plurality of nucleic acid molecules form an array of nucleic acid molecules and wherein said cells into which the nucleic acid molecules are introduced form an array of cells comprising the nucleic acid molecules. (google.com)
  • 10. A nucleic acid molecule encoding a polypeptide according to claim 1. (freepatentsonline.com)
  • 11. A nucleic acid molecule encoding a polypeptide according to claim 1 and a CPU prepro sequence. (freepatentsonline.com)
  • Equine connective tissue tumors contain unintegrated bovine papilloma virus DNA. (microbiologyresearch.org)
  • The cytoskeleton plays an important role in abnormal growth, invasion, and metastasis, which are characteristics of malignant tumors ( 1 , 2 ). (aacrjournals.org)
  • A type of human papillomavirus especially associated with malignant tumors of the genital and RESPIRATORY MUCOSA. (bioportfolio.com)
  • 2. Phylogeny and Taxonomy of Papillomaviruses. (caister.com)
  • BPV-4 causes squamous cell carcinomas of the alimentary tract, and BPV-1/2 causes carcinomas and haemangioendotheliomas of the urinary bladder, in both cases in animals that have fed on bracken (Pteridium aquilinum). (wikipedia.org)
  • The ISH for BPV type 1 DNA intensely stained the nuclei of neoplastic mesenchymal spindle cells. (oregonstate.edu)
  • Experimental research of immunotherapeutic protocols is carried out on murine models of tumours in which human papillomaviruses are known to play a role. (cuni.cz)
  • Papillomaviruses (PVs) are highly epitheliotropic as they usually establish productive infections within squamous epithelia of the skin, the anogenital tract and the oral cavity. (nih.gov)
  • This study shows that both active and productive infections by BPV-2 in the urothelium of the bovine and bubaline urinary bladder can occur in vivo. (nih.gov)
  • The human papillomaviruses (HPV) came into the play as members of a family of animal tumor viruses and because of their known association with genital infections. (caister.com)