Borrelia Infections: Infections with bacteria of the genus BORRELIA.Borrelia burgdorferi: A specific species of bacteria, part of the BORRELIA BURGDORFERI GROUP, whose common name is Lyme disease spirochete.Borrelia: A genus of gram-negative, anaerobic, helical bacteria, various species of which produce RELAPSING FEVER in humans and other animals.Tick Infestations: Infestations with soft-bodied (Argasidae) or hard-bodied (Ixodidae) ticks.Lyme Disease: An infectious disease caused by a spirochete, BORRELIA BURGDORFERI, which is transmitted chiefly by Ixodes dammini (see IXODES) and pacificus ticks in the United States and Ixodes ricinis (see IXODES) in Europe. It is a disease with early and late cutaneous manifestations plus involvement of the nervous system, heart, eye, and joints in variable combinations. The disease was formerly known as Lyme arthritis and first discovered at Old Lyme, Connecticut.Ixodes: The largest genus of TICKS in the family IXODIDAE, containing over 200 species. Many infest humans and other mammals and several are vectors of diseases such as LYME DISEASE, tick-borne encephalitis (ENCEPHALITIS, TICK-BORNE), and KYASANUR FOREST DISEASE.Relapsing Fever: An acute infection characterized by recurrent episodes of PYREXIA alternating with asymptomatic intervals of apparent recovery. This condition is caused by SPIROCHETES of the genus BORRELIA. It is transmitted by the BITES of either the body louse (PEDICULUS humanus corporis), for which humans are the reservoir, or by soft ticks of the genus ORNITHODOROS, for which rodents and other animals are the principal reservoirs.Ticks: Blood-sucking acarid parasites of the order Ixodida comprising two families: the softbacked ticks (ARGASIDAE) and hardbacked ticks (IXODIDAE). Ticks are larger than their relatives, the MITES. They penetrate the skin of their host by means of highly specialized, hooked mouth parts and feed on its blood. Ticks attack all groups of terrestrial vertebrates. In humans they are responsible for many TICK-BORNE DISEASES, including the transmission of ROCKY MOUNTAIN SPOTTED FEVER; TULAREMIA; BABESIOSIS; AFRICAN SWINE FEVER; and RELAPSING FEVER. (From Barnes, Invertebrate Zoology, 5th ed, pp543-44)Arachnid Vectors: Members of the class Arachnida, especially SPIDERS; SCORPIONS; MITES; and TICKS; which transmit infective organisms from one host to another or from an inanimate reservoir to an animate host.Bacterial Outer Membrane Proteins: Proteins isolated from the outer membrane of Gram-negative bacteria.Bacterial Vaccines: Suspensions of attenuated or killed bacteria administered for the prevention or treatment of infectious bacterial disease.Lyme Disease Vaccines: Vaccines or candidate vaccines used to prevent LYME DISEASE.Lipoproteins: Lipid-protein complexes involved in the transportation and metabolism of lipids in the body. They are spherical particles consisting of a hydrophobic core of TRIGLYCERIDES and CHOLESTEROL ESTERS surrounded by a layer of hydrophilic free CHOLESTEROL; PHOSPHOLIPIDS; and APOLIPOPROTEINS. Lipoproteins are classified by their varying buoyant density and sizes.Antibodies, Bacterial: Immunoglobulins produced in a response to BACTERIAL ANTIGENS.Antigens, Bacterial: Substances elaborated by bacteria that have antigenic activity.Erythema Chronicum Migrans: A deep type of gyrate erythema that follows a bite by an ixodid tick; it is a stage-1 manifestation of LYME DISEASE. The site of the bite is characterized by a red papule that expands peripherally as a nonscaling, palpable band that clears centrally. This condition is often associated with systemic symptoms such as chills, fever, headache, malaise, nausea, vomiting, fatigue, backache, and stiff neck.Peromyscus: A genus of the subfamily SIGMODONTINAE consisting of 49 species. Two of these are widely used in medical research. They are P. leucopus, or the white-footed mouse, and P. maniculatus, or the deer mouse.Lyme Neuroborreliosis: Nervous system infections caused by tick-borne spirochetes of the BORRELIA BURGDORFERI GROUP. The disease may affect elements of the central or peripheral nervous system in isolation or in combination. Common clinical manifestations include a lymphocytic meningitis, cranial neuropathy (most often a facial neuropathy), POLYRADICULOPATHY, and a mild loss of memory and other cognitive functions. Less often more extensive inflammation involving the central nervous system (encephalomyelitis) may occur. In the peripheral nervous system, B. burgdorferi infection is associated with mononeuritis multiplex and polyradiculoneuritis. (From J Neurol Sci 1998 Jan 8;153(2):182-91)Mice, Inbred C3HDNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Flagellin: A protein with a molecular weight of 40,000 isolated from bacterial flagella. At appropriate pH and salt concentration, three flagellin monomers can spontaneously reaggregate to form structures which appear identical to intact flagella.Antigens, Surface: Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.Spirochaetales: An order of slender, flexuous, helically coiled bacteria, with one or more complete turns in the helix.Ornithodoros: A genus of softbacked TICKS, in the family ARGASIDAE, serving as the vector of BORRELIA, causing RELAPSING FEVER, and of the AFRICAN SWINE FEVER VIRUS.Disease Reservoirs: Animate or inanimate sources which normally harbor disease-causing organisms and thus serve as potential sources of disease outbreaks. Reservoirs are distinguished from vectors (DISEASE VECTORS) and carriers, which are agents of disease transmission rather than continuing sources of potential disease outbreaks.Tick-Borne Diseases: Bacterial, viral, or parasitic diseases transmitted to humans and animals by the bite of infected ticks. The families Ixodidae and Argasidae contain many bloodsucking species that are important pests of man and domestic birds and mammals and probably exceed all other arthropods in the number and variety of disease agents they transmit. Many of the tick-borne diseases are zoonotic.Histiocytic Sarcoma: Malignant neoplasms composed of MACROPHAGES or DENDRITIC CELLS. Most histiocytic sarcomas present as localized tumor masses without a leukemic phase. Though the biological behavior of these neoplasms resemble lymphomas, their cell lineage is histiocytic not lymphoid.Dog Diseases: Diseases of the domestic dog (Canis familiaris). This term does not include diseases of wild dogs, WOLVES; FOXES; and other Canidae for which the heading CARNIVORA is used.Borrelia burgdorferi Group: Gram-negative helical bacteria, in the genus BORRELIA, that are the etiologic agents of LYME DISEASE. The group comprises many specific species including Borrelia afzelii, Borellia garinii, and BORRELIA BURGDORFERI proper. These spirochetes are generally transmitted by several species of ixodid ticks.Dogs: The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)Facial NeoplasmsNymph: The immature stage in the life cycle of those orders of insects characterized by gradual metamorphosis, in which the young resemble the imago in general form of body, including compound eyes and external wings; also the 8-legged stage of mites and ticks that follows the first moult.Transaminases: A subclass of enzymes of the transferase class that catalyze the transfer of an amino group from a donor (generally an amino acid) to an acceptor (generally a 2-keto acid). Most of these enzymes are pyridoxyl phosphate proteins. (Dorland, 28th ed) EC 2.6.1.Membrane Proteins: Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Immunization, Passive: Transfer of immunity from immunized to non-immune host by administration of serum antibodies, or transplantation of lymphocytes (ADOPTIVE TRANSFER).Genetics, Population: The discipline studying genetic composition of populations and effects of factors such as GENETIC SELECTION, population size, MUTATION, migration, and GENETIC DRIFT on the frequencies of various GENOTYPES and PHENOTYPES using a variety of GENETIC TECHNIQUES.Algorithms: A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.Genetic Variation: Genotypic differences observed among individuals in a population.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Arthritis, Infectious: Arthritis caused by BACTERIA; RICKETTSIA; MYCOPLASMA; VIRUSES; FUNGI; or PARASITES.Gene Expression Regulation, Bacterial: Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.Genes, Bacterial: The functional hereditary units of BACTERIA.Gene Expression Profiling: The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.Bacterial Proteins: Proteins found in any species of bacterium.Robotics: The application of electronic, computerized control systems to mechanical devices designed to perform human functions. Formerly restricted to industry, but nowadays applied to artificial organs controlled by bionic (bioelectronic) devices, like automated insulin pumps and other prostheses.Menu PlanningVolleyball: A team sport in which two teams hit an inflated ball back and forth over a high net using their hands.Professional Corporations: Legally authorized corporations owned and managed by one or more professionals (medical, dental, legal) in which the income is ascribed primarily to the professional activities of the owners or stockholders.Immunoassay: A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.Amiodarone: An antianginal and class III antiarrhythmic drug. It increases the duration of ventricular and atrial muscle action by inhibiting POTASSIUM CHANNELS and VOLTAGE-GATED SODIUM CHANNELS. There is a resulting decrease in heart rate and in vascular resistance.

Specific antibodies reactive with the 22-kilodalton major outer surface protein of Borrelia anserina Ni-NL protect chicks from infection. (1/122)

An outer surface lipoprotein of 22 kDa was identified in the avian pathogen Borrelia anserina Ni-NL by using antibody preparations reactive with bacterial surface-exposed proteins. Amino acid sequence analysis of the 22-kDa protein demonstrated 90% identity with VmpA of B. turicatae, suggesting that the protein belongs to the family of 20-kDa outer surface proteins of the genus Borrelia. All of the 60 chicks intramuscularly treated with antibodies specifically reacting with the 22-kDa protein and infected with strain Ni-NL were completely protected from infection, since no spirochetemia was detected, and from death. Control chicks were treated with immune sera raised against apathogenic strain B. anserina Es, which expresses a prominent 20-kDa polypeptide that is also a member of the Vmp family but does not cross-react immunologically with the 22-kDa protein of the Ni-NL strain. These animals, infected with B. anserina Ni-NL, showed a high degree of spirochetemia 10 days after infection, and all died between 14 and 21 days after infection. The results showed that the 22-kDa surface protein of B. anserina Ni-NL is a determinant of the pathogenic potential of the strain and also confirmed that only strain-specific antibodies are protective against B. anserina infection.  (+info)

Comparative analysis and immunological characterization of the Borrelia Bdr protein family. (2/122)

Multiple circular and linear plasmids of Lyme disease and relapsing fever Borrelia spirochetes carry genes for members of the Bdr (Borrelia direct repeat) protein family. To define their common and divergent attributes, we first comprehensively compared the known homologs. Bdr proteins with predicted sizes ranging from 10.7 to 30. 6 kDa formed five homology groups, based on variable numbers of short direct repeats in a central domain and diverse N- and C-terminal domains. In a further characterization, Western blots were probed with rabbit antisera raised against either of two purified recombinant Bdr proteins from Borrelia burgdorferi B31. The results showed that antibodies cross-react and several Bdr paralogs 19.5 to 30.5 kDa in size are expressed by cultured strain B31 in a temperature-independent manner. In situ proteolysis, immunofluorescence, and growth inhibition assays indicated that Bdr proteins are not surface exposed. Distinct patterns of cross-reacting proteins of 17.5 to 33 kDa were also detected in other B. burgdorferi, Borrelia garinii, and Borrelia afzelii strains as well as in relapsing fever spirochetes Borrelia hermsii and Borrelia turicatae. Last, we examined whether these proteins are antibody targets during Lyme disease. Analysis of 47 Lyme disease patient sera by immunoblotting and enzyme-linked immunosorbent assays showed that 24 (51%) and 20 (43%), respectively, had detectable antibodies to one or more of the Bdr proteins. Together, these data indicate that Bdr proteins constitute a family of cross-reactive Borrelia proteins which are expressed in the course of Lyme disease and in vitro.  (+info)

Characterization of VspB of Borrelia turicatae, a major outer membrane protein expressed in blood and tissues of mice. (3/122)

Serotypes A and B of the relapsing fever spirochete Borrelia turicatae produce different disease manifestations in infected mice. Whereas serotype B causes more severe arthritis and reaches higher densities in the blood of mice than serotype A, serotype A invades the central nervous system earlier than serotype B during infection. These differences between serotypes A and B in mice are associated with the expression of different surface proteins, VspA and VspB, respectively, in the culture medium. To determine whether these proteins, in particular, VspB, are also expressed in vivo, scid mice infected with B. turicatae were studied. The expression of VspB by spirochetes in the blood was demonstrated in Coomassie blue-stained polyacrylamide gels and Western blots with a specific monoclonal antibody. Indirect immunofluorescence and immunoperoxidase studies confirmed the expression of VspB in the blood and also demonstrated VspB expression in the joints and heart. The gene for VspB was next identified and cloned by using partial amino acid sequencing, reverse transcriptase PCR, and a specific monoclonal antibody. The vspB gene encodes a protein of 216 amino acids that is 68% identical to VspA of B. turicatae and 44 to 56% identical to representative Vsp and OspC lipoproteins of other Borrelia spp. The processed VspB protein was distinguished from 26 other Vsp and OspC proteins by a high predicted isoelectric point at 9.39. The promoter region for vspB was similar to the promoter region for the vsp33 gene of Borrelia hermsii and for the ospC gene of Borrelia burgdorferi, two genes known to be environmentally regulated. These studies established that the virulence-associated VspB protein is expressed by spirochetes in the mouse and that VspB is a novel member of the Vsp-OspC family of proteins.  (+info)

Survey of three bacterial louse-associated diseases among rural Andean communities in Peru: prevalence of epidemic typhus, trench fever, and relapsing fever. (4/122)

Typhus and other louse-transmitted bacterial infections in Peruvian sierra communities are known to occur but have not recently been assessed. In this study, 194 of 1,280 inhabitants of four villages in Calca Province in the Urubamba Valley were included. Thirty-nine (20%) of the 194 volunteers had antibodies to Rickettsia prowazekii, whereas 24 (12%) had antibodies to Bartonella quintana and 2 against Borrelia recurrentis. There was a significant correlation between the presence of infesting ectoparasites and antibodies to R. prowazekii, as well as between antibodies to R. prowazekii and ectoparasite infestation and fever in the previous 6 months. The proportion of inhabitants infested with ectoparasites was significantly higher in the highest-altitude village than in the other three villages. Two volunteers' antibody levels suggested a recent typhus infection, but only B. quintana DNA was amplified from lice. Epidemic typhus remains extant in the area, and B. quintana infections were encountered and documented for the first time in South America.  (+info)

Successful in vitro cultivation of Borrelia duttonii and its comparison with Borrelia recurrentis. (5/122)

Borrelia duttonii, the cause of East African tick-borne relapsing fever, has until now been refractory to growth in laboratory media. This spirochaete has only be propagated in mice or by tissue culture, restricting both yield and purity of cells available for research. The successful isolation of five clinical isolates of B. duttonii from patients in Central Tanzania and their comparison with Borrelia recurrentis is reported. Electron microscopy revealed spirochaetal cells with pointed ends, a mean wavelength of 1.8 microns with an amplitude of 0.8 micron, similar to the findings for B. recurrentis. Cells contained 10 periplasmic flagella inserted at each end of the spirochaete, again comparable with the counts of 8-10 flagella found in B. recurrentis. PFGE revealed a chromosome of approximately 1 Mb, a large plasmid of approximately 200 kb, and a small plasmid of 11 kb in all strains of B. duttonii and in B. recurrentis. B. duttonii possessed a further 7-9 plasmids with sizes ranging from 20 to 90 kb. In two isolates of B. duttonii, the profiles were identical. In contrast, all 18 isolates of B. recurrentis fell into one of five plasmid patterns with 3-4 plasmids ranging from 25 to 61.5 kb in addition to those of 11 and 200 kb described above. Analysis of the SDS-PAGE profiles of B. duttonii strains revealed a high-molecular-mass band of 33.4-34.2 kDa in four strains (variable large protein, VLP) and a low-molecular-mass band of 22.3 kDa in the remaining strain (variable small protein, VSP). This resembles the protein profiles found in B. recurrentis. The G + C ratio of B. duttonii was 27.6 mol%. Nucleotide sequence of the rrs gene (16S rRNA) from four B. duttonii isolates revealed 100% identity among these strains and 99.7% homology with three strains deposited by others in GenBank. The rrs gene of eight representative clinical isolates of B. recurrentis confirmed their close similarity with B. duttonii.  (+info)

Borrelia isolates in Northern Colorado identified as Borrelia bissettii. (6/122)

Previous work described Borrelia burgdorferi sensu lato group DN127 as a new genospecies, Borrelia bissettii, and prompted the present study to identify the Borrelia spp. that exist in northern Colorado. To determine the genospecies present, we analyzed two specific intergenic spacer regions located between the 5S and 23S and the 16S and 23S ribosomal genes. Phylogenetic analysis of the derived sequences clearly demonstrated that these isolates, originating from rodents captured in the foothills of northern Colorado, diverged from B. burgdorferi sensu stricto by 5 to 5.5% and were members of the new genospecies B. bissettii.  (+info)

Serodiagnosis of Louse-Borne relapsing fever with glycerophosphodiester phosphodiesterase (GlpQ) from Borrelia recurrentis. (7/122)

Human louse-borne relapsing fever occurs in sporadic outbreaks in central and eastern Africa that are characterized by significant morbidity and mortality. Isolates of the causative agent, Borrelia recurrentis, were obtained from the blood of four patients during a recent epidemic of the disease in southern Sudan. The glpQ gene, encoding glycerophosphodiester phosphodiesterase, from these isolates was sequenced and compared with the glpQ sequences obtained from other relapsing-fever spirochetes. Previously we showed that GlpQ of Borrelia hermsii is an immunogenic protein with utility as a serological test antigen for discriminating tick-borne relapsing fever from Lyme disease. In the present work, we cloned and expressed the glpQ gene from B. recurrentis and used recombinant GlpQ in serological tests. Acute- and convalescent-phase serum samples obtained from 42 patients with louse-borne relapsing fever were tested with an indirect immunofluorescence assay (IFA) and an enzyme-linked immunosorbent assay (ELISA) that used whole cells of B. recurrentis and with immunoblotting to whole-cell lysates of the spirochete and Escherichia coli producing recombinant GlpQ. The geometric mean titers of the acute- and convalescent-phase serum samples measured by IFA were 1:83 and 1:575, respectively. The immunoblot analysis identified a high level of reactivity and seroconversion to GlpQ, and the assay was more sensitive than the whole-cell IFA and ELISA using purified, recombinant histidine-tagged GlpQ. Serum antibodies to GlpQ and other antigens persisted for 27 years in one patient. We conclude that assessment of anti-GlpQ antibodies will allow serological confirmation of louse-borne relapsing fever and determination of disease prevalence.  (+info)

Lone star tick-infecting borreliae are most closely related to the agent of bovine borreliosis. (8/122)

Although Borrelia theileri, the agent of bovine borreliosis, was described at the turn of the century (in 1903), its relationship with borreliae causing Lyme disease or relapsing fever remains undescribed. We tested the previously published hypothesis that spirochetes infecting Lone Star ticks (Amblyomma americanum) may comprise B. theileri by analyzing the 16S ribosomal DNAs (rDNAs) and flagellin genes of these spirochetes. B. theileri, the Amblyomma agent, and B. miyamotoi formed a natural group or clade distinct from but most closely related to that of the relapsing fever spirochetes. B. theileri and the Amblyomma agent were 97 and 98% similar at the nucleotide level within the analyzed portions of the 16S rDNA and the flagellin gene respectively, suggesting a recent divergence. The agent of bovine borreliosis might be explored as a surrogate antigen for the as-yet-uncultivatable Amblyomma agent in studies designed to explore the etiology of a Lyme disease-like infection associated with Lone Star ticks.  (+info)

A newly described illness of the southern United States, closely related to Lyme disease, caused by Borrelia lonestari. It is associated with the bite of the Lone Star Tick, Amblyomma americanum.
Although the Lone Star Tick (Amblyomma americanum) has been shown to transmit Lyme borreliosis (Borrelia americana and Borrelia andersonii), it can transmit other infections such as Ehrlichia and Tularemia.. In addition, victims bitten by the Lone Star Tick will occasionally develop a circular rash, similar to the rash of early Lyme disease. The rash may be accompanied by flu-like symptoms, and this condition has been named "Southern Tick-Associated Rash Illness" (STARI). STARI is treated with oral antibiotics. Newer research is now associating a Lone Star Tick bite to a mammalian meat allergy.. ...
Aim: Here we investigated the role of complement activation in phagocytosis and the release of cytokines and chemokines in response to two clinical isolates: Borrelia afzelii K78, which is resistant to complement-mediated lysis, and Borrelia garinii LU59, which is complement-sensitive.. Methods: Borrelia spirochetes were incubated in hirudin plasma, or hirudin-anticoagulated whole blood. Complement activation was measured as the generation of C3a and sC5b-9. Binding of the complement components C3, factor H, C4, and C4BP to the bacterial surfaces was analyzed. The importance of complement activation on phagocytosis, and on the release of cytokines and chemokines, was investigated using inhibitors acting at different levels of the complement cascade.. Results: 1) Borrelia garinii LU59 induced significantly higher complement activation than did Borrelia afzelii K78. 2) Borrelia afzelii K78 recruited higher amounts of factor H resulting in significantly lower C3 binding. 3) Both Borrelia strains ...
Would you have Transaminases increased when you have Borrelia infection? We study people who have Transaminases increased and Borrelia infection from FDA. Check out who they are, other conditions they have and drugs they take
Introduction: Lyme disease is a tickborne illness that generates controversy among medical providers and researchers. One of the key topics of debate is the existence of persistent infection with the Lyme spirochete, Borrelia burgdorferi, in patients who have been treated with recommended doses of antibiotics yet remain symptomatic. Persistent spirochetal infection despite antibiotic therapy has recently been demonstrated in non-human primates. We present evidence of persistent Borrelia infection despite antibiotic therapy in patients with ongoing Lyme disease symptoms. Materials & Methods: In this pilot study, culture of body fluids and tissues was performed in a randomly selected group of 12 patients with persistent Lyme disease symptoms who had been treated or who were being treated with antibiotics. Cultures were also performed on a group of 10 control subjects without Lyme disease. The cultures were subjected to corroborative microscopic, histopathological and molecular testing for Borrelia
Lyme Borreliosis is an infectious disease caused by the spirochete Borrelia burgdorferi that is transmitted through the bite of infected ticks. Both B cell-mediated humoral immunity and T cell immunity develop during natural Borrelia infection. However, compared with humoral immunity, the T cell response to Borrelia infection has not been well elucidated. In this study, a novel T cell-based assay was developed and validated for the sensitive detection of antigen-specific T cell response to B. burgdorferi. Using interferon-g as a biomarker, we developed a new enzyme-linked immunospot method (iSpot Lyme™) to detect Borrelia antigen-specific effector/memory T cells that were activated in vivo by exposing them to recombinant Borrelia antigens ex vivo. To test this new method as a potential laboratory diagnostic tool, we performed a clinical study with a cohort of Borrelia positive patients and healthy controls. We demonstrated that the iSpot Lyme assay has a significantly higher specificity and
Author Summary Lyme borreliosis displays multifaceted clinical manifestations caused by the Borrelia burgdorferi sensu lato complex. If insufficiently treated, infection may proceed to inflammatory complications of chronic infection. Th17-like cytokines, foremost IL-17 and IL-22, are crucial for host defense against extracellular bacteria. IL-17/IL-22 secretion by human leukocytes exposed to live Borreliae has not been analyzed. Here we report that B. burgdorferi-activated PBMC lack immediate IL-17 expression despite being highly activated and robust T cell-dependent production of IL-22 that to a large part is mediated by monocyte-derived IL-1. Early innate immunity may shape dermal infection, thus likely affecting bacterial dissemination. Specifically, insufficient neutrophil recruitment/function, supposedly due to insufficient early IL-17 production along with a lack of opsonizing antibodies, may favor the spread of B. burgdorferi. Indeed, neutrophilic inflammation, indicative of IL-17 bioactivity, is
Malaria is a common disease in tropical Africa, causing between 1.5 and 2.7 million deaths each year. As a rule, only the clinical symptoms are used for quick diagnosis in order to prescribe the proper treatment. However, this type of presumptive diagnosis is problematic, as there are other diseases that have the same symptoms, such as relapsing fever (an infection caused by bacteria of the Borrelia genus).. How the patient is affect by this was previously unknown, and, to study the phenomenon, an animal model was created for this type of double infection. It turned out that when both diseases occur at the same time the malaria is much milder whereas the Borrelia infection in turn is more serious, indeed, fatal. This is because the immune defense focuses on the malaria infection, which means that the relapsing fever can grow unhampered. Mice with double infection develop severe anemia and serious internal damage, above all in the spleen, which is important for the immune defense. Malaria can ...
Borrelia bacteria (Borrelia sp.), coloured transmission electron micrograph (TEM). These bacteria are pathogenic, causing diseases in humans and in animals. They are transmitted by arthropods such as ticks. Lyme disease and relapsing fever are two diseases that are caused by Borrelia bacteria. The bacteria are long, coiling rope-like structures. Magnification: x3550 when printed 10cm high. - Stock Image B220/1528
The aim was to find if there is variability in the production of specific antibodies by BALB/c mice to certain antigens of three individual Borrelia afzelii (dead cell suspension) strains originally isolated from different sources. Analysis of the borrelian proteins immunogenicity was performed...
Enzyme immunoassay for detection of IgG antibodies to Borrelia burgdorferi in human serum, plasma, synovial or cerebrospinal fluid and for the detection of intrathecal production of specific antibodies to Borrelia sp.
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AIM: To study the ability of OspC recombinant proteins from Borrelia garinii and Borrelia afzelii isolated in West Siberia to interact with serum antibodies from patients with tick-borne borreliosis (TBB). MATERIALS AND METHODS: Recombinant antigens OspC B. garinii and OspC B. afrelii, serum samples from patients with TBB were used as well as solid-phase enzyme immunoassay and parametric and non-parametric statistical methods. RESULTS: Higher antigenic activity of B. garinii OspC compared with OspC from B. afzelii was observed when these recombinant proteins were compared in enzyme immunoassay. Detection rate of class M and G immunoglobulins to B. garinii OspC in sera of patients with TBB was 60.5% and 70% respectively. CONCLUSION: Obtained results indicate high immunoreactivity of OspC recombinant proteins from B. garinii and B. afzelii and point to perspective of their combined use for serological diagnostics of TBB.
BACKGROUND: Morphea is an inflammatory autoimmune skin sclerosis of unknownetiology. A causative role of Borrelia burgdorferi infection has beencontroversially discussed, but no conclusive solution has yet been achieved. OBJECTIVE: Intrigued by 3 young patients with severe Borrelia-associated morpheaand high-titer antinuclear antibodies, we retrospectively examined therelationship between Borrelia exposure, serologic autoimmune phenomena and ageat disease onset in morphea patients. METHODS: In 90 morphea patients thepresence of Borrelia-specific serum antibodies was correlated to the age atdisease onset and the presence and titers of antinuclear antibodies. Patientswith active Borrelia infection or high-titer antinuclear antibodies due tosystemic sclerosis or lupus erythematosus served as controls. RESULTS: We observed a statistically highly significant association between morphea, serologic evidence of Borrelia infection, and high-titer antinuclear antibodies when disease onset was in childhood ...
Gelderblom H, Londoño D, Bai Y, Cabral ES, Quandt J, Hornung R, Martin R, Marques A, Cadavid D. High production of CXCL13 in blood and brain during persistent infection with the relapsing fever spirochete Borrelia turicatae. J Neuropathol Exp Neurol. 2007 Mar; 66(3):208-17 ...
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Borrelia afzelii NlpH protein: isolated from Borrelia afzelii; amino acid sequence in first source; NlpH protein also found in B. burgdorferi and B. garinii.; GenBank Y08413
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In 2001, Dr. Raphael Stricker, a San Francisco Hematologist, was largely responsible for identifying abnormal CD 57 cell number in chronic Lyme Disease patients. He and his associates documented that those patients exhibiting significant neurological symptoms had lower CD57 cell counts than those with musculoskeletal symptoms. Additionally, it was found that once the underlying Borrelia infection began to be treated, CD57 cell number increased. Dr. Strickers work suggests that decreased CD57 cells may reflect an immune defect induced by the Borrelia spirochete that allows infection to persist, or that a pre-existing immunodeficiency of natural killer cells predisposes patients to be more prone to contracting Lyme Disease ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
We recently compared diagnostic parameters of different commercial serological kits based on three different antigen types and correlated test results with the status of the patients Borrelia infection (1). We thank Lacout et al. for comments on our article expressed in their comment letter (2).. Their objections mostly concern controversial issues, such as persistent polymorphic symptomatology after tick-bite (PPSTB), posttreatment Lyme disease syndrome (PTLDS), persistent or chronic Borrelia disease, etc., discussed in laboratory and clinical diagnostics of Lyme borreliosis for more than 30 years. These are extensive issues, which, in our opinion, are outside the scope of the original communication, so in a brief comment, there is no space for a qualified discussion on this controversial topic.. The selection of patients and their inclusion in clinical groups were performed in accordance with the national (3) and European (4, 5) standards and case definitions. Only patients with a typical, ...
bacterial Vsp protein: from Borrelia turicatae expressed in blood & tissues of mice; amino acid sequence in first source; GenBank AF048952
Lyme disease is a bacterial (Borrelia) infection passed on through the bite of an infected tick. It has become the most common tick-borne infection in many parts of Europe and the USA.. Early signs may include a rash around the area of the bite (erythema migrans), with flu-like and/or neurological symptoms,* but symptoms can vary or not be apparent. Without prompt antibiotic treatment, however, permanent joint and/or nerve damage is a risk.. Higher incidence rates of Lyme disease in neighbouring countries have prompted fears that current estimates of the annual number of new cases in the UK (2000 to 3000), which are based on laboratory data for England and Wales, might be too low.. To explore this further, a team of researchers drew on anonymised medical information submitted by family doctors to the nationally representative Clinical Practice Research Datalink (CPRD) between 2001 and 2012.. This database covers around 8.4 million people from 658 general practices, equivalent to around 8% of the ...
Enzyme immunoassay for the detection of IgG antibodies to B. garinii in human serum, plasma, synovial or cerebrospinal fluid and for the detection of intrathecal production of specific antibodies to Borrelia sp. SmartEIA kit is specifically designed for automated analysis using the Agility instrument.
Most medical professionals, when speaking of Lyme disease, are typically referring to an outdated and over-simplified version of the disease that was once taught and believed that the main causative agent being dealt with was the borrelia spirochete. We now know there are many agents involved. Borreliosis Complex for Lyme disease is a better term than just Lyme disease. We now know there are many agents involved and much neuroendocrine involvement due to not only Borrelia, but viral activity and damage to the Methylation cycle (KREBS CYCLE). It is so good to see the two groups learning from one another. Initially the group of Lyme researchers was looking at only Borrelia the causative agent for Lyme disease but as time went on they discovered many other agents. The CFIDS doctors were looking at damage to the Methylation cycle, Diastolic Heart Dysfunction and so much more in their research endeavors. It appears one group was looking for the AGENTS and the other was looking for the DAMAGE. It is ...
Two horses at Lone Star Park have tested positive for Equine Piroplasmosis. In addition, a third horse that left Lone Star and had been shipped to New Mexico also tested positive.
Carman, R J. and Wright, D J., "Agglutinating antibody in murine borrelia duttoni infection. Abstr." (1982). Subject Strain Bibliography 1982. 1265 ...
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The yearly event benefiting the Lone Star Paralysis Foundation will showcase Austins most popular chefs as well as handcrafted cocktails from Patron Spirits Company, Treaty Oak Distillery, and The Tipsy ...
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Borrelia sp. phylogenetically different from Lyme disease- and relapsing fever-related Borrelia spp. in Amblyomma varanense from Python reticulatus. Crossref DOI link: https://doi.org/10.1186/S13071-016-1629-8 Published: 2016-12. Update policy: https://doi.org/10.1007/SPRINGER_CROSSMARK_POLICY. ...
Lone Star College System consists of five colleges, including LSC-CyFair, LSC-Kingwood, LSC-Montgomery, LSC-North Harris, and LSC-Tomball, six centers and Lone Star College-University Center.
To estimate the basic reproduction number (R(0)) of Borrelia lusitaniae and Borrelia afzelii, we formulated a mathematical model considering the interactions among the tick vector, vertebrate hosts, and pathogens in a 500-ha enclosed natural reserve on Le Cerbaie hills, Tuscany, central Italy. In the study area, Ixodes ricinus were abundant and were found infected by B. lusitaniae and B. afzelii. Lizards (Podarcis spp.) and mice (Apodemus spp.), respectively, are the reservoir hosts of these two Borrelia burgdorferi sensu lato (s.l.) genospecies and compete for immature ticks. B. lusitaniae R(0) estimation is in agreement with field observations, indicating the maintenance and diffusion of this genospecies in the study area, where lizards are abundant and highly infested by I. ricinus immature stages. In fact, B. lusitaniae shows a focal distribution in areas where the tick vector and the vertebrate reservoir coexist. Mouse population dynamics and their relatively low suitability as hosts for nymphs
Borrelia hermsii is the most common cause of tickborne relapsing fever in North America. DNA sequences of the 16S-23S rDNA noncoding intergenic spacer (IGS) region were determined for 37 isolates of this spirochete. These sequences distinguished the 2 genomic groups of B. hermsii identified previously with other loci. Multiple IGS genotypes were identified among isolates from an island, which suggested that birds might play a role in dispersing these spirochetes in nature. In support of this theory, all stages of the tick vector Ornithodoros hermsi fed successfully on birds in the laboratory and advanced in their life cycle. B. hermsii produced a detectable spirochetemia in 1 chicken inoculated subcutaneously. Additional work is warranted to explore the role of birds as enzootic hosts for this relapsing fever spirochete ...
Borrelia burgdorferi, the causative agent of Lyme disease, has long been known to be capable of forming aggregates and colonies. It was recently demonstrated that Borrelia burgdorferi aggregate formation dramatically changes the in vitro response to hostile environments by this pathogen. In this study, we investigated the hypothesis that these aggregates are indeed biofilms, structures whose resistance to unfavorable conditions are well documented. We studied Borrelia burgdorferi for several known hallmark features of biofilm, including structural rearrangements in the aggregates, variations in development on various substrate matrices and secretion of a protective extracellular polymeric substance (EPS) matrix using several modes of microscopic, cell and molecular biology techniques. The atomic force microscopic results provided evidence that multilevel rearrangements take place at different stages of aggregate development, producing a complex, continuously rearranging structure. Our results also
The Borrelia burgdorferi (Lyme) IgG ELISA Kit is intended for the measurement of IgG antibodies to Borrelia burgdorferi (Lyme) in a sample. This kit utilizes Borrelia sensu stricto ATCC 35211 complete antigen.
These findings support the hypothesis that there is an association between Borrelia burgdorferi infection and psychiatric morbidity. In countries where this infection is endemic, a proportion of psychiatric inpatients may be suffering from neuropathogenic effects of Borrelia burgdorferi.
I read Branda and Rosenbergs (1) editorial with great interest. Readers may be interested to know that my colleagues and I discovered Borrelia miyamotoi in Ixodes scapularis ticks more than 10 years ago and were able to show transmission by infected ticks to mice with subsequent antibody response and persistent infection (2). We also showed that this organism was widespread in I. scapularis, with a mean prevalence of about 2%. We then applied 5 unsuccessful times to the National Institutes of Health and the Centers for Disease Control and Prevention for funding to determine whether this organism infects humans and causes disease. The agencies cited lack of evidence for human infection as their reasoning for not funding our study, which was the objective of the proposal ...
Neighbor-joining phylogenetic tree (bootstrap value 250) showingclustering of the rrs gene between Borrelia duttonii/B. recurrentis andB. crocidurae.
vlp1B. recurrentis A1 is plasmid encoded and duplicated in isolate A1. A: Plasmid (lane 1 to 4) and chromosome-rich (lane 5 to 8) DNA were digested with EcoRI (
And [we] cant even make a [blood] smear with Borrelia burgdorferi and see the organism. Its there. But you dont see it. You cannot find this spirochete. Why not? After all, I have a sick person here. He is trembling all over. His synovial fluid is full of spirochetes. But when it comes to blood, its not there. So there is something associated with this organism that makes it different. Andy Wilson: Why is Borrelia burgdorferi so hard to find in the body and culture outside the body? Dr. Burgdorfer: Borrelia burgdorferi in the tissues of a patient is extremely difficult to demonstrate, because, first of all, you dont like somebody to take samples out of your brain [to look] for spirochetes. The same with other tissues. Every system in your body can be infected with spirochete. But to prove that is extremely difficult. It demands surgical work, which is very expensive Andy Wilson: Are you a believer in the idea of persistent Lyme infections? Dr. Burgdorfer: I am a believer in persistent ...
And [we] cant even make a [blood] smear with Borrelia burgdorferi and see the organism. Its there. But you dont see it. You cannot find this spirochete. Why not? After all, I have a sick person here. He is trembling all over. His synovial fluid is full of spirochetes. But when it comes to blood, its not there. So there is something associated with this organism that makes it different. Andy Wilson: Why is Borrelia burgdorferi so hard to find in the body and culture outside the body? Dr. Burgdorfer: Borrelia burgdorferi in the tissues of a patient is extremely difficult to demonstrate, because, first of all, you dont like somebody to take samples out of your brain [to look] for spirochetes. The same with other tissues. Every system in your body can be infected with spirochete. But to prove that is extremely difficult. It demands surgical work, which is very expensive Andy Wilson: Are you a believer in the idea of persistent Lyme infections? Dr. Burgdorfer: I am a believer in persistent ...
Relapsing Fever has other names including Relapsing fever borreliosis, louse-borne relapsing fever (LBRF, also known as epidemic relapsing fever), tick-borne relapsing fever (TBRF)
This listing is for an Adobe Pdf file of a crochet pattern for a Female Lone Star Tick. Please note that this is a pattern only, the finished item is not included.
There are 404 calories in 1 order (4.6 oz) of Lone Star Steakhouse & Saloon Baby Back Ribs Specialty. Youd need to walk 106 minutes to burn 404 calories. Visit CalorieKing to see calorie count and nutrient data for all portion sizes.
Find information about Lone Star College Tomball community health classes. Completion of an accredited nursing program or other medical training can qualify you to start working in a private hospital immediately.
The Ranchers Surprise Triplets (Lone Star Cowboy League: Multiple Blessings) by Linda Ford - book cover, description, publication history.
All organisms, from bacteria to humans, need to recognize where they are, and respond accordingly. A pathogen must sense its location in the inf...
Kan katten ha drabbats av borrelia? Av HemmetsJournal , Publicerad Artikel 1 av 10 Kan katten ha drabbats av borrelia? När den glomerulära proteinurin enbart består av albuminuri utan samtidigt läckage av större proteiner benämns tillståndet selektiv proteinuri. Hej. Har fått enalapril sandoz 5mg utskrivet,för att jag har protein i urinen. Har typ2 diabetes. Har väldigt svårt att hitta någon som helst information om att denna . Urinen kan också vara grumlig eller ha en obehaglig lukt. Om blåsan också har Självtestet påvisar protein, nitrit och/eller leukocyter i urinen. Leukocyter. For complete product information refer to the product insert. In a free catch urine sample - which I am assuming is what you are collecting - there is a protein chance you get bacteria or cells in the urine sample from the vagina or prepuce. Quote:Oh and kennel cough is actually a bacteria, one thing I did discover was acne instagram most control groups of dogs were given - at that weight class - they ...
Grill Master and World Travelled Chef Stuart Rackham joins us to tell us about some great grilling tips. Chef Rackham works for Lone Star steakhouse, which first opened in Winston-Salem, North Carolina in 1989. To get more information about Lone Star, you can check out their site at: http://www.
Your at home Lyme Disease test, testing for Borrelia levels in your blood. Order online, collect your samples from the comfort of home, return to the lab & secure results online | NL
The maintenance of Borrelia burgdorferi in a population of Peromyscus leucopus was investigated from 202 mark and recapture mice and 61 mice that were removed from a site in Baltimore County, Maryland. Borrelia burgdorferi infection was detected by culture and polymerase chain reaction (PCR) of ear tissue, and exposure to the spirochete was quantified by serology. Overall prevalence of B. burgdorferi, as determined by culture and PCR of ear tissue at first capture, was 25% in the longitudinal sample and 42% in the cross-sectional sample. Significantly more juvenile mice were captured in the longitudinal sample (18%) than in the cross-sectional sample (0%). Among 36 captured juvenile mice, only one was infected with B. burgdorferi; this contributed to a significant trend for infection with B. burgdorferi with age. Recovery from infection with B. burgdorferi was not detected among 77 mice followed for an average of 160 days. The incidence rate of infection with B. burgdorferi was 10 times greater in mice
Four patients who had received tick bites while visiting forests in Mexico had skin lesions that met the case definition of erythema migrans, or borrelial lymphocytoma. Clinical diagnosis was supported with histologic, serologic, and molecular tests. This study suggests the Borrelia burgdorferi infection is in Mexico.
Five persons contracted tick-borne relapsing fever after staying in a cabin in western Montana. Borrelia hermsii was isolated from the blood of two patients, and Ornithodoros hermsi ticks were collected from the cabin, the first demonstration of this bacterium and tick in Montana. Relapsing fever should be considered when patients who reside or have vacationed in western Montana exhibit a recurring febrile illness.
Borrelia burgdorferi is a pathogenic spirochete which cycles between its arthropod vector and vertebrate host. If transmitted to humans, B. burgdorferi causes Lyme disease, an infection which can impair different organs, such as the skin, joints, nervous system and heart. Alterations in protein expression due to the different environments Borrelia encounters during its complicated life cycle require advanced adaptation mechanisms. The outer surface-exposed proteins play a critical role in survival and pathogenesis of Borrelia in different hosts and tissues, being involved in avoiding the host immune response, adhesion to different tissues and nutrient acquisition. This thesis aimed to characterize integral outer membrane proteins which play a role in solute and nutrient uptake, and provides support for their role in the environmental adaptation of Borrelia.. In this thesis, three B. burgdorferi proteins, P13, BBA01 and P66, were shown to be porins, and characterized structurally and functionally ...
The bite of the Ixodes tick species is responsible for transmitting Borrelia miyamotoi, just as it is responsible for Lyme disease, babesiosis, and anaplasmosis.
The persistence of Borrelia burgdorferi in patients treated with antibiotics is described. The diagnosis of Lyme disease is based on clinical symptoms, epidemiology and specific IgG and IgM antibody titers to B. burgdorferi in serum. Antibiotic therapy may abrogate the antibody response to the infection as shown in our patients. B. burgdorferi may persist as shown by positive culture in MKP-medium; patients may have subclinical or clinical disease without diagnostic antibody titers to B. burgdorferi. We conclude that early stage of the disease as well as chronic Lyme disease with persistence of B. burgdorferi after antibiotic therapy cannot be excluded when the serum is negative for antibodies against B. burgdorferi ...
Borrelia burgdorferi ATCC ® 35210D-5™ Designation: Genomic DNA from Borrelia burgdorferi Strain B31 TypeStrain=True Application: Vector borne research
Metabolism:. This organism is an extracellular organism. It adapts to the host organism accordingly. The nutrient requirement of borrelia is very specific hence it makes the survival of this organism in vitro very difficult. It acts mainly by the regulation of the various lipoproteins present in the body.. The Lyme disease or the recurring fever that is caused due to borrelia mainly occur due to a tick or a flee bite. The life span of a tick is about 2 years. In order to survive it requires a blood meal. The blood meal helps the tick to mature. Spirochetes are acquired by the tick mostly in the larva stage. The blood meal in order to survive is acquired from mice and other types of rodents such as the white footed mice. The spirochetes then survive on the expense of the tick. The tick acts as the host. The spirochetes generally survive in the digestive track of the host through which it passes the disease to other animals and human beings.. Human beings are generally affected when they get ...
The persistence of Borrelia burgdorferi in patients treated with antibiotics is described. The diagnosis of Lyme disease is based on clinical symptoms, epidemiology and specific IgG and IgM antibody t
SCHERTZ - A Seattle-based pet food supplier has acquired wholesale pet product distributor Lone Star Pet Supply Inc. for an undisclosed sum, top officials at both companies said Friday. The buyer, Animal Supply Co., said in a statement that the purchase of Lone Star Pet will enable it to sell products from 300 manufacturers to 4,000 pet retail stores in 20 states, expanding its reach to six new states. Lone Star Pet now has 93 employees at its warehouse and offices on Triton Drive, and no layoffs are planned.
Biomedica offers ELISA kits for the diagnostic screening of Lyme Borreliosis - Borrelia recombinant IgG ELISA and Borrelia recombinant IgM ELISA. The combination of antigens used in both Borrelia assays detect antibodies directed against the different Borrelia species, pathogenic for humans, including B. burgdorferi senso strictu.
A Novel Laboratory Assessment for the Detection of Borrelia Burgdorferi by Chenggang Jin, MD, PhD, Pharmasan Labs, and Deanna J. Fall, BA, NeuroScience Inc. ** Townsend Letter, the Examiner of Alternative Medicine alternative medicine magazine articles are written by researchers, health practitioners and patients.
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Million Hearts is a national movement with an ambitious goal of preventing 1 million heart attacks and strokes over 5 years. The U.S. Department of Health and Human Services (HHS), a partner in Million Hearts, recently recognized Lone Star Circle of Care as a national Million Hearts leader based on the organizations…. ...
Opens the Highlight Feature Bar and highlights feature annotations from the FEATURES table of the record. The Highlight Feature Bar can be used to navigate to and highlight other features and provides links to display the highlighted region separately. Links in the FEATURES table will also highlight the corresponding region of the sequence. More... ...
Opens the Highlight Feature Bar and highlights feature annotations from the FEATURES table of the record. The Highlight Feature Bar can be used to navigate to and highlight other features and provides links to display the highlighted region separately. Links in the FEATURES table will also highlight the corresponding region of the sequence. More... ...
Because Lone Star® Hi Pro Plus® is concentrated nutrition, you can feed less than most other dog foods. Food requirements will vary depending on your dogs age, activity, and condition. Monitor your dogs weight. If you notice an overweight or underweight condition, vary the amount of Hi Pro Plus accordingly. Your dog will still be getting all of the nutrition he needs. Also, be sure to provide adequate fresh water in a clean bowl for your dog every day.. Lone Star Hi Pro Plus can be fed dry or mixed with water, milk, or broth. If mixed, we suggest starting with one part liquid to four parts Hi Pro Plus. Then adjust this ratio as preferred by your dog.. PUPPIES: Start puppies on Lone Star Hi Pro Plus as early as they begin to nibble on solid foods (usually 3 to 5 weeks of age). While they continue nursing, keep moistened Hi Pro Plus available at all times until your puppies are fully weaned at 6 to 8 weeks of age. After weaning, Hi Pro Plus may be fed moist or dry. Feed pups up to 6 months of ...
Nicień , Źródło: scienceblogs.com Patolog Dr Alan MacDonald wykrył nicienie podczas autopsji tkanek mózgu pacjentów chorych na borelioze Lyme, stwardnienie rozsiane, demencje, glejaka mózgu oraz chorobę Alzheimera. Dr MacDonald wykazał również iż krętki borrelia ukrywają się we wnętrzu pasożytniczych nicieni, powodując przewlekłe choroby mózgu ,znalazł on trzy szczepy bakterii borrelia żyjących w pasożytniczych nicieniach,…
Relapsing fever is a tick-borne bacterial disease caused by a number of species in the genus Borrelia. Persistence is a key component of the lifecycle of relapsing fever Borrelia as it improves the chance for tick acquisition ...
A simple method has been found that tells people who have become seriously ill after a tick bite once and for all whether they have bacteria in their blood.
Had you heard the news out of ASM2014 (American Society for Microbiology)? Kim Lewis from Northeastern has been doing research on what are called persisters (bacteria that can survive antibiotics without being killed). He had been granted about $6M in NIH (National Institute of Health) funding plus was supported by the Bill and Melinda Gates Foundation for chronic TB due to persisters. ...
Willy Burgdorfer, who discovered the Lyme spirochete which was later named for him (Borrelia burgdorferi, died this week at age 89.
By Robbie Blakeley, Contributing Sports Reporter RIO DE JANEIRO, BRAZIL - This week, we continue our series profiling Rio de Janeiros biggest football (soccer) clubs with the short story behind the founding of Botafogo. Known as Estrela Solitaria (The Lone Star), they were once Brazils most famous and successful
Christi Craddick was elected Texas Railroad Commissioner in 2012 and has held her seat for two terms. She works in Austin to assure the oil, gas, and railroad industries of Texas are operating at their top performance. Experiences in oil and gas law, in combination with a parent as a politician, has contributed to her knowledge of being an elected official. Born and raised in Midland, Texas, Christi grew up in the heat and surrounded by conservative republicans. Because of father, Tom Craddick, has been a Texas Congressman since before her parents were married, Christi was exposed to the life of a politician and the duties that comes with it. That never stopped her father from putting his family first, she says, and it was his example of family first that has inspired her to work hard but always come home to her family. Despite the time that requires her to work away from home, Christi places emphasis on time spent with her family. ...
Borrelia burgdorferi is a bacterial species of the spirochete class of the genus Borrelia. B. burgdorferi exists in North America and Europe and is the only causative agent of Lyme disease in the United States. Borrelia species are considered diderm (double-membrane) bacteria rather than Gram-positive or negative. Borrelia burgdorferi is named after the researcher Willy Burgdorfer, who first isolated the bacterium in 1982. Borrelia species in the species complex known to cause Lyme disease are collectively called Borrelia burgdorferi sensu lato (s.l.) not to be confused with this single species Borrelia burgdorferi sensu stricto in that complex which is responsible for all cases of Lyme disease in North America. B. burgdorferi resembles other spirochetes in that it has an outer membrane and inner membrane with a thin layer of peptidoglycan in between. However, the outer membrane lacks lipopolysaccharide. Its shape is a flat wave. It is about 0.3 μm wide and 5 to 20 μm in length. B. burgdorferi ...
Lyme disease, or borreliosis, is caused by spirochetal bacteria from the genus Borrelia, which has 52 known species. Three main species (Borrelia garinii, Borrelia afzelii, and Borrelia burgdorferi s.s.) are the main causative agents of the disease in humans, while a number of others have been implicated as possibly pathogenic. Borrelia species in the species complex known to cause Lyme disease are collectively called Borrelia burgdorferi sensu lato (s.l.) not to be confused with the single species in that complex Borrelia burgdorferi sensu stricto which is responsible for all cases of Lyme disease in North America. Borrelia are microaerophilic and slow-growing-the primary reason for the long delays when diagnosing Lyme disease-and have been found to have greater strain diversity than previously estimated. The strains differ in clinical symptoms and/or presentation as well as geographic distribution. Except for Borrelia recurrentis (which causes louse-borne relapsing fever and is transmitted by ...
Abstract In order to determine if Peromyscus spp. could become infected with the Lyme disease spirochete (Borrelia burgdorferi) by direct inoculation and to determine the duration of spirochetemia, 4 P. leucopus and 5 P. maniculatus were inoculated by the intramuscular, intraperitoneal, and subcutaneous routes with an isolate of B. burgdorferi obtained from the blood of a trapped wild P. leucopus from Camp McCoy, Wisconsin. All of the mice developed antibodies to B. burgdorferi which reached a peak indirect immunofluorescent (IFA) geometric mean antibody titer of 10 log2 21 days post-inoculation. B. burgdorferi was recovered from the blood of 1 P. maniculatus 21 days post-inoculation. One uninfected Peromyscus of each species was housed in the same cage with the infected Peromyscus as a contact control. Both of the contact controls developed IFA B. burgdorferi antibodies by day 14, indicating contact infection. To determine if B. burgdorferi was being transmitted by direct contact, 5 uninfected P.
Unrecognized and untreated Borrelia infection can progress from localized inflammation (erythema migrans) to early or late generalized stage within weeks to months. Meningoradiculitis, arthritis, multiple erythemas, myositis, and myocarditis of the early generalized stage have a good prognosis after antibiotic treatment, but late manifestations can progress to chronic disease. Phrenic nerve palsy, autonomic nervous system dysfunction and carditis with acute heart failure are among rare manifestations as well as late generalised stage with myelitis. We present a case of a patient with meningoradiculitis, autonomic nervous dysfunction, respiratory failure due to phrenic nerve palsy and acute heart failure with systolic myocardial dysfunction. The diagnosis of Borrelia infection was confirmed by positive serological testing, appropriate response to antibiotic therapy and exclusion of other diseases. Our case suggests that in unexplained respiratory failure and acute systolic myocardial dysfunction, ...
Borrelia burgdorferi sensu lato is a diverse bacterial group with a worldwide distribution of 20 named and 1 unnamed genospecies. B. burgdorferi sensu stricto seen in North America, B. afzelii and B. garinii in Europe, are the main genospecies (group 1) that cause Lyme disease. A second group of eight genospecies (B. bavariensis, B. bissettii, B. kurtenbachii, B. mayonii, B. miyamotoi, B lusitaniae, B spielmanii, B valisiana) have been shown to have a limited disease potential.
Borrelia burgdorferi, the Lyme disease agent, and other members of the spirochetal genus Borrelia have double-stranded linear plasmids in addition to supercoiled circular plasmids. The copy number relative to the chromosome was determined for 49- and 16-kb linear plasmids and a 27-kb circular plasmid of the type strain, B31, of B. burgdorferi. All three plasmids were present in low copy number, about one per chromosome equivalent, as determined by relative hybridizations of replicon-specific DNA probes. The low copy number of Borrelia plasmids suggests that initiation of DNA replication and partitioning are carefully controlled during the cell division cycle. The copy numbers of these three plasmids of strain B31 were unchanged after approximately 7,000 generations in continuous in vitro culture. A clone of B. burgdorferi B31 that did not contain the 16-kb linear plasmid was obtained after exposure of a culture to novobiocin, a DNA gyrase inhibitor. The plasmid-cured strain contains only one ...
Borrelia burgdorferi synthesizes an HtrA protease (BbHtrA) which is a surface-exposed, conserved protein within Lyme disease spirochetes with activity toward CheX and BmpD of Borrelia spp, as well as aggrecan, fibronectin and proteoglycans found in skin, joints and neural tissues of vertebrates. An antibody response against BbHtrA is observed in Lyme disease patients and in experimentally infected laboratory mice and rabbits. Given the surface location of BbHtrA on B. burgdorferi and its ability to elicit an antibody response in infected hosts, we explored recombinant BbHtrA as a potential vaccine candidate in a mouse model of tick-transmitted Lyme disease. We immunized mice with two forms of BbHtrA: the proteolytically active native form and BbHtrA ablated of activity by a serine to alanine mutation at amino acid 226 (BbHtrAS226A). Although inoculation with either BbHtrA or BbHtrAS226A produced high-titer antibody responses in C3H/HeJ mice, neither antigen was successful in protecting mice from ...
Relapsing fevers occur worldwide and are characterized by recurrent episodes of fever and spirochetemia. In central, eastern, and southern Africa, the disease is often caused by Borrelia duttonii, which is transmitted by the soft tick Ornithodoros moubata. We conducted a field investigation in September 1994 at a hospital in Mitwaba, southern Zaire, which was the only medical facility within 150 km. The introduction of a rapid blood-smear staining technique allowed us to demonstrate that 4.3%-7.4% of the 25-50 new outpatients seen each day had relapsing fever. Because of the absence of malaria in this area, these patients account for most of the febrile patients. The incidence of relapsing fever among all pregnant women in the maternity ward was estimated to be 6.4%, and this condition often led to maternal death or to spontaneous abortion. The 16S rRNA gene of B. dutonii was sequenced after the spirochete was isolated from patients blood samples and directly from Ornithodoros tick vectors. In ...
Infectious diseases such as malaria and relapsing fever borreliosis (RF), cause severe human mortality and morbidity in developing countries. Malaria, caused by Plasmodium spp. parasites, is estimated by the World Health Organization to cause 1.5-2.7 million deaths annually. RF, caused by Borrelia spirochetes, has the highest prevalence described for any bacterial disease in Africa, with infection outcomes ranging from asymptomatic to fatal. RF borreliosis manifests in humans as a recurring fever and with other symptoms very similar to those of malaria.. RF borreliosis has been regarded as a transient infection of the blood. However, B. duttonii exploits the brain as an immunoprivileged site escaping the host immune response while spirochetes in the blood are cleared. To investigate whether residual bacteria are dormant or actively dividing, mice with residual brain infection were administered ceftriaxone, a β-lactam antibiotic interfering with cell wall synthesis. Hence, it only affects ...
In this study, more than 3400 short-term B. burgdorferi T cell lines generated from subjects with chronic B. burgdorferi infection were compared with naive B. burgdorferi-reactive T cell lines from normal individuals. Chronic B. burgdorferi infection was associated with high frequencies of B. burgdorferi-reactive T cells secreting high amounts of IFN-γ and IL-10, which was induced by IL-12 secreted by PBMC. T cell secretion of IFN-γ and IL-10 was suppressed in the presence of neutralizing Abs to IL-12. The presence of IFN-γ/IL-10 secretion was observed only in B. burgdorferi- and not in TT-specific T cell lines. These data demonstrate that chronic B. burgdorferi infection causes IL-12 secretion that induces a population of T cells characterized by secretion of both IFN-γ and IL-10.. The significance of the cellular immune response to B. burgdorferi in Lyme disease has been investigated earlier. A strong T cell response to B. burgdorferi was detected early in the course of illness, often ...
Bacteria produce only two types of toxins: endotoxins, which are non-secreted lipopolysaccharides (LPSs) that make up a large part of the cell wall of gram-negative bacteria; and, exotoxins that are secreted by some gram-positive bacteria and a few strains of gram-negative bacteria.. At one time, Borrelia burgdorferi, was thought to possess an endotoxin since a product isolated from B. burgdorferi was reported to be pyrogenic for rabbits, mitogenic for human mononuclear cells and mouse spleen cells, capable of clotting limulus lysate (a diagnostic test for LPS), and cytotoxic for mouse macrophages; these are properties generally ascribed to bacterial LPS (1). However, subsequent studies revealed the absence of lipid A and other chemical structures characteristic of classic gram-negative endotoxins (2). Although B. burgdorferi does not produce an endotoxin, it does possess lipoproteins that interact with Toll-like receptors (TLRs) on the surface of mammalian cells that comprise the innate immune ...
Borrelia burgdorferi is the causative agent of Lyme disease in North America and is transmitted by ticks of the genus Ixodes. It is a highly invasive spirochete...
The Borrelia burgdorferi bacteria causes Lyme disease, but many people continue to suffer even when all the B. burgodorferi are dead - a previously unexplained phenomenon called chronic Lyme disease…
Lone star ticks, (Amblyoma americanum). are taking over. They now comprise more than 80% of the small black legged, hard bodied ticks found in the D.C metro area and elsewhere. These guys are very aggressive. They may be hard to distinguish from their deer tick (Ixodes scapularis) colleagues. Adult females are easy to spot: white spot on top. The shape and coloration is somewhat different. Take a careful look with a magnifying glass and compare to pix on google images. The CDC party line is: Lone stars do not transmit Lyme; they transmit STARI which is a mild disease and easy to treat. The CDC website states it is unknown wich bacteria causes the syndrome. The CDC website says Borrelia lonstari was a suspect but "further research" showed this not to be the case. This "definitive" research is the product of a small study published by Gary Wormser (name familiar) in 2005. Thirty EM rashes were examined for B. burdorferi (classic Lyme) and B. lonestari. Wormser did not find the genetic signature of ...
Antigens, Bacterial, CHEMISTRY, GENETICS, Bacterial Outer Membrane Proteins, CHEMISTRY, GENETICS, Borrelia burgdorferi, CHEMISTRY, IMMUNOLOGY, Carbon Isotopes, Escherichia coli, GENETICS, Nitrogen Isotopes, Nuclear Magnetic Resonance, Biomolecular, Protons, Recombinant Proteins, CHEMISTRY, Solutions, Support, U.S. Govt, P.H.S ...
Borrelia burgdorferi ATCC ® 35210™ Designation: B31 TypeStrain=True Application: Emerging infectious disease research Vector borne research
Quidel Corporation, a provider of rapid diagnostic testing solutions, cellular-based virology assays and molecular diagnostic systems, announced today that it has received CE Mark to market Quidels Sofia 2 Lyme+ FIA to be used with the Sofia 2 Fluorescent Immunoassay Analyzer for the rapid differential detection of human IgM and IgG antibodies to Borrelia burgdorferi, Borrelia garinii, and Borrelia afzelii from serum and plasma specimens.. ...
Borrelia burgdorferi, the Lyme disease-causing spirochete, is often found associated with host connective tissue, where it interacts with components of the extracellular matrix, including fibronectin. BBK32 is a surface-expressed lipoprotein with fibronectin-binding ability of Borrelia burgdorferi. A fragment of the bbk32 gene of Borrelia afzelii strain ACAI encoding the N-terminus of the protein including the fibronectin-binding domain (designated BS4 in this study) was cloned end expressed in Echerichia coli under the control of arabinose promoter as six histidine-tagged protein. Expression for the target protein showed that BS4 was accumulated both in soluble and insoluble forms. The molecular weight of the recombinant protein was estimated by SDS-PAGE to be 35 kDa including the six histidine tag. The expressed protein was purified by Ni2+ affinity chromatography under denaturing conditions. The purified BS4 recombinant protein was evaluated as an antigen in the serology of Lyme disease. ...
Borrelia has also been isolated in Mongolia. In Europe, Lyme disease is caused by infection with one or more pathogenic ... Borrelia burgdorferi and Borrelia mayonii are the cause. In Europe and Asia, the bacteria Borrelia afzelii and Borrelia garinii ... Within days to weeks after the onset of local infection, the Borrelia bacteria may begin to spread through the bloodstream. EM ... The diagnosis of neurologic infection by Borrelia should not be excluded solely on the basis of normal routine CSF or negative ...
1989). "Survival of Borrelia burgdorferi in antibiotically treated patients with Lyme borreliosis". Infection. 17 (6): 355-9. ... Borrelia Borrelia burgdorferi B31 Genome Page Borrelia garinii PBi Genome Page Borrelia afzelli PKo Genome Page Schwan TG, ... Three main species (Borrelia garinii, Borrelia afzelii, and Borrelia burgdorferi s.s.) are the main causative agents of the ... "Expanded diversity among Californian Borrelia isolates and description of Borrelia bissettii sp. nov. (formerly Borrelia group ...
In 1987, LS linked with Borrelia infection. Lichen sclerosus et atrophicus was first described in 1887 by Dr. Hallopeau. Since ... Eisendle, K; Grabner, TG; Kutzner, H (2008). "Possible Role of Borrelia burgdorferi Sensu Lato Infection in Lichen Sclerosus". ... A link with Lyme Disease is shown by the presence of Borrelia burgdorferi in LSA biopsy tissue. Since LS in females is ... Yashar, S; Han, KF; Haley, JC (2004). "Lichen sclerosus-lichen planus overlap in a patient with hepatitis C virus infection". ...
"Borrelia burgdorferi OspC Protein Required Exclusively in a Crucial Early Stage of Mammalian Infection". Infection and Immunity ... "Temporal Analysis of the Antigenic Composition of Borrelia burgdorferi during Infection in Rabbit Skin". Infection and Immunity ... "Innate immunity networks during infection with Borrelia burgdorferi". Critical Reviews in Microbiology. 42 (2): 233-244. doi: ... OspA from Borrelia burgdorferi is an unusual outer surface protein, it has two globular domains which are connected with a ...
It is caused by infection with Borrelia burgdorferi. Types include: Acrodermatitis enteropathica Acropustulosis Acrodermatitis ... It may also be associated with hepatitis B and other viral infections. The lesions appear as small coppery-red, flat-topped ...
Eskow E, Rao RV, Mordechai E (Sep 2001). "Concurrent infection of the central nervous system by Borrelia burgdorferi and ... Infection cycle[edit]. The currently accepted model explaining the infection cycle holds that the transmitting vectors are ... Bartonella infections are remarkable in the wide range of symptoms they can produce. The course of the diseases (acute or ... Immediately after infection, the bacteria colonize a primary niche, the endothelial cells. Every five days, some of the ...
James AM, Liveris D, Wormser GP, Schwartz I, Montecalvo MA, Johnson BJ (June 2001). "Borrelia lonestari infection after a bite ... Borrelia Zoonosis Schulze TL, Bowen GS, Bosler EM, et al. (May 1984). "Amblyomma americanum: a potential vector of Lyme disease ... Infections are treated with antibiotics, particularly doxycycline, and the acute symptoms appear to respond to these drugs. No ... It is spread by tick bites, but the organism that causes the infection is unknown. This illness is a tick-borne disease carried ...
Such target lesions (bull's-eye rashes) are characteristic of Borrelia infections, and no other pathogens are known that cause ... "Borrelia lonestari infection after a bite by an Amblyomma americanum tick". J. Infect. Dis. 183 (12): 1810-4. doi:10.1086/ ... but rather an actual skin infection of one of the Lyme bacteria species from the genus Borrelia. "Erythema migrans is the only ... The EM rash occurs in 80% to 90% of those infected with Borrelia. A systematic review of the medical literature showed 80% of ...
"First record of natural infection with Borrelia in Ornithodoros (Ornithodoros) savignyi. Reservoir potential and specificity of ... It is also a carrier of Borrelia, a spirochaete bacterium, and is a carrier of a flavivirus causing Alkhurma hemorrhagic fever ... the tick to Borrelia". Journal of the Egyptian Society of Parasitology. 30 (3): 765-80. PMID 11198375. Charrel, RN; Fagbo, S; ...
... while the bacterial infections include Mycoplasma pneumoniae, Borrelia burgdorferi, Leptospira, and beta-hemolytic Streptococci ... For a rubella infection, the risk is 1 per 5,000 cases. Some early vaccines, later shown to have been contaminated with host ... Viral infections thought to induce ADEM include influenza virus, enterovirus, measles, mumps, rubella, varicella zoster, ... It is often triggered after the patient has received a viral infection or, perhaps exceedingly rarely specific non-routine ...
2004) Borrelia burgdorferi infection in a natural population of Peromyscus leucopus mice: a longitudinal study in an area where ... 2001) Effects of acorn production and mouse abundance on abundance and Borrelia burgdorferi infection prevalence of nymphal ... Hofmeister EK, Ellis BA, Glass GE & Childs JE (1999) Longitudinal study of infection with Borrelia burgdorferi in a population ... Systemic disease in Peromyscus leucopus associated with Borrelia burgdorferi infection. Am J Trop Med Hyg ; 42:254-259 ...
"Borrelia burgdorferi central nervous system infection presenting as an organic schizophrenialike disorder". Biol. Psychiatry. ... infectious and postinfectious syndromes, including infections causing delirium, viral encephalitis, HIV/AIDS,[34] malaria,[35] ... Steinberg, D.; S.R. Hirsch; S.D. Marston; K. Reynolds; R.N. Sutton (May 1972). "Influenza infection causing manic psychosis". ... Full blood count including ESR to rule out a systemic infection or chronic disease, and ...
"Borrelia burgdorferi central nervous system infection presenting as an organic schizophrenialike disorder". 1999년 3월 15일.. ...
Lyme disease; is a bacterial infection caused by Borrelia burgdorferi, a spirochete bacterium transmitted by the Deer tick ( ... Depression after infection with West Nile virus Murray KO, Resnick M, Miller V. Depression after infection with West Nile virus ... Humans can be infected in 3 different ways: ingestion of tissue cysts, ingestion of oocysts, or in utero infection with ... CS1 maint: Multiple names: authors list (link) Carruthers VB, Suzuki Y (May 2007). "Effects of Toxoplasma gondii Infection on ...
"Coiling phagocytosis is the preferential phagocytic mechanism for Borrelia burgdorferi". Infection and Immunity. 60 (10): 4205- ... In the United States, about 2 infections with L. pneumophila appear per 100,000 residents per year[not in citation given]. The ... Michod, RE; Bernstein, H; Nedelcu, AM (2008). "Adaptive value of sex in microbial pathogens" (PDF). Infection, Genetics and ... Infection and Immunity. 81 (7): 2598-2605. doi:10.1128/IAI.00296-13. PMC 3697626 . PMID 23649096. Creasey, E. A.; Isberg, R. R ...
Lyme disease, caused by chronic Borrelia burgdorferi infection, is a common cause of facial nerve paralysis in endemic areas. ... Otitis media is an infection in the middle ear, which can spread to the facial nerve and inflame it, causing compression of the ... There is no known cause of Bell's palsy, although it has been associated with herpes simplex infection. Bell's palsy may ...
... neuroborreliosis is caused by a systemic infection of spirochetes of the genus Borrelia. Symptoms of the disease include ... The microbiological progression of the disease is similar to that of neurosyphilis, another spirochetal infection. ... From Infection to Inflammation". Molecular Medicine. 14 (3-4): 205-12. doi:10.2119/2007-00091.Rupprecht (inactive 2017-01-15). ... but antibiotic sensitivities of prevailing European and US isolates of Borrelia burgdorferi tend to be identical. However, ...
The disease is caused by an infection of Borrelia burgdorferi, a tick-borne spirochete bacterium also responsible for causing ...
Faulde MK, Robbins RG (February 2008). "Tick infestation risk and Borrelia burgdorferi s.l. infection-induced increase in host- ... There is some evidence that Ixodes ricinus infected with Borrelia burgdorferi may become more efficient at infestation. Home ...
This progressive skin process is due to the effect of continuing active infection with the spirochete Borrelia afzelii, which ... B. afzelii may not be the exclusive etiologic agent of ACA; Borrelia garinii has also been detected. The rash caused by ACA is ...
It is possible for a tick to carry and transmit one of the co-infections and not Borrelia, making diagnosis difficult and often ... Transtadial (between tick stages) passage of Borrelia burgdorferi is common. Vertical passage (from mother to egg) of Borrelia ... It can also transmit other Borrelia species, including Borrelia miyamotoi. Ticks that transmit B. burgdorferi to humans can ... One of the keys of the success of I. scapularis as a Borrelia vector relies on its ability to limit the proliferation of the ...
"5-Lipoxygenase negatively regulates Th1 response during Brucella abortus infection in mice". Infection and Immunity. 83 (3): ... Borrelia burgdorferi, and Paracoccidioides brasiliensis. In a model of cecum perforation-induced sepsis, ALOX5 gene knockout ... Alox5 Gene knockout mice are more susceptible to the development and pathological complications of experimental infection with ... Basil MC, Levy BD (2016). "Specialized pro-resolving mediators: endogenous regulators of infection and inflammation". Nature ...
Ledum was able to almost completely inactivate the tick-borne bacterial infection caused by Borrelia, involved in the ...
He believed Borrelia burgdorferi was a persistent infection, and that the current serological testing methodologies needed to ... As a research subject for his thesis he chose to study the development of the African relapsing fever spirochete, Borrelia ... He discovered the bacterial pathogen that causes Lyme disease, a spirochete named Borrelia burgdorferi in his honor. He was ... The agent was named after him - Borrelia burgdorferi. Throughout his career, Burgdorfer participated in a number of World ...
... antibody production and long-term protection after bacterial infection such as Borrelia hermsii and Streptococcus pneumoniae. ...
It is caused by bacteria of the genus Borrelia. The disease is carried by ticks which are parasitic on mammals such as mice and ... It is the most common tick-borne infection in the United States. Although Allen Steere realized in 1978 that Lyme disease was a ...
Borrelia Infections. Gram-Negative Bacterial Infections. Bacterial Infections. Tick-Borne Diseases. Skin Diseases, Bacterial. ... Spirochaetales Infections. Skin Diseases, Infectious. Infection. Tongue Diseases. Mouth Diseases. Stomatognathic Diseases. ...
Reactivation of Borrelia infection in birds.. Gylfe A1, Bergström S, Lundström J, Olsen B. ...
55 Studies found for: lyme AND Borrelia Infections. Also searched for Lyme Disease, Infectious, and Borreliosis. See Search ... NGS detection of Borrelia burgdorferi DNA in blood compared to serology. *Changes in NGS detection of Borrelia burgdorferi DNA ... Detection of Borrelia Bacteria in Early Stage Lyme Borreliosis Using the T2Lyme Panel. *Lyme Disease ... A Multicenter Study to Evaluate a Borrelia Diagnostic Test in Subjects With Early Stage or Late Stage Lyme Disease. *Lyme ...
This data illustrate the potent, if temporal, immune suppression induced by Borrelia-infection. Collectively, the data reveal a ... Moreover, influenza vaccine antigens, when applied during Borrelia-infection, failed to induce strong antibody responses and ... This inability to induce long-term immunity was not due to the nature of Borrelia antigens, as even T-dependent antigens of ... We show here that this is because the immune system of the Borrelia-infected host generates only short-lived, structurally ...
... 12.07.2007. Borrelia burgdorferi seem to be particularly ... However Gerber says that "the findings in the present study are unique as infections with B. burgdorferi are not causing ... The biological reasons behind infection with the bacteria and the consequences for Bernese Mountain Dogs are currently unknown. ... burgdorferi infection may mean it coincides with glomerulonephritis without being the cause of the disease. ...
In view of a lack of standardisation of borrelia serology, the clinician must expect false negative, and--even more so--false- ... The laboratory diagnosis of Borrelia burgdorferi infection.. By Vogt A • ProHealth.com • April 10, 1990 ... In view of a lack of standardisation of borrelia serology, the clinician must expect false negative, and-even more so-false- ...
All patientswere treated for Borrelia infection and all of them developed psychotic disorders. History of each patient was ... Psychosis in Borrelia burgdorferi infection - part II: case reports. Psychiatr Pol. 2019 Jun 30;53(3):641-653 Authors: ... Psychosis in Borrelia burgdorferi infection - part II: case reports. Psychiatr Pol. 2019 Jun 30;53(3):641-653 Authors: ... All patientswere treated for Borrelia infection and all of them developed psychotic disorders. History of each patient was ...
Rather than defining a set disease pattern, however, one should, more logically, conceptualize a progressive infection that may ... Erythema migrans, the earliest and most easily recognized manifestation of B burgdorferi infection, is an expanding annular ... Invasion of the central nervous system occurs as the infection disseminates hematogenously, with encephalitis, myelitis and ... Early antibiotic treatment of the infection is highly effective. ... caused by the spirochete Borrelia burgdorferi, has classically ...
Lyme arthritis provides a mouse model for exploring the development of pathology following infection of C3H mice with Borrelia ... Borrelia burgdorferi infection induces lipid mediator production during Lyme arthritis. Biochimie. 2017 Jun 16;: Authors: Brown ... Borrelia burgdorferi infection induces lipid mediator production during Lyme arthritis. Biochimie. 2017 Jun 16;: Authors: Brown ... More News: Arthritis , Autoimmune Disease , Biochemistry , Borrelia , Cancer , Cancer & Oncology , Lyme Disease , Pathology , ...
Borrelia burgdorferi σ54 is required for mammalian infection and vector transmission but not for tick colonization. Mark A. ... Borrelia burgdorferi σ54 is required for mammalian infection and vector transmission but not for tick colonization ... Borrelia burgdorferi σ54 is required for mammalian infection and vector transmission but not for tick colonization ... Borrelia burgdorferi σ54 is required for mammalian infection and vector transmission but not for tick colonization ...
We confirmed infection of 2 patients with Borrelia miyamotoi in Japan by retrospective surveillance of Lyme disease patients ... Krause PJ, Narasimhan S, Wormser GP, Rollend L, Fikrig E, Lepore T, Human Borrelia miyamotoi infection in the United States. N ... Saito K, Ito T, Asashima N, Ohno M, Nagai R, Fujita H, Case report: Borrelia valaisiana infection in a Japanese man associated ... Chowdri HR, Gugliotta JL, Berardi VP, Goethert HK, Molloy PJ, Sterling SL, Borrelia miyamotoi infection presenting as human ...
Borrelia burgdorferi expression of the bba64, bba65, bba66, and bba73 genes in tissues during persistent infection in mice.. ... Borrelia burgdorferi, the etiological agent of Lyme disease in humans, is vectored between mammalian hosts in nature by Ixodes ... bba64 expression was highly upregulated in bladder, heart, and spleen tissues throughout the infection period, contrasting with ... bba65, bba66, and bba73 demonstrated upregulated differential expression in various tissues over 1 year post-infection. These ...
The presence of B. miyamotoi in the patients blood was confirmed by PCR, and phylogenetic analysis identified an infection ... We report a human case of Borrelia miyamotoi infection diagnosed in Austria. Spirochetes were detected in Giemsa-stained blood ... Borrelia miyamotoi infection in nature and in humans. Clin Microbiol Infect. 2015;21:631-9. DOIPubMed ... We report a human case of Borrelia miyamotoi infection diagnosed in Austria. Spirochetes were detected in Giemsa-stained blood ...
After infection and distribution by the bloodstream, relapsing fever episodes occur in humans. Since no reliable in vivo tools ... With the successful infection model presented here, further studies are conceivable in order to gain advanced insights into the ... Current migration of vast numbers of individuals from this area increases the likelihood that B. persica infections will be ... of new rapid diagnostic approaches to initiate or monitor treatment regimes in order to clear or prevent the infection with B. ...
... referred to our Department for the serologic diagnosis of Borrelia infection. The patients sera were tested for Borrelia- ... Antinuclear antibodies are not increased in the early phase of Borrelia infection. Posted By wp_admin. On January 1, 2004 @ 12: ... In the literature, there are case reports suggesting that Borrelia burgdorferi infection may induce autoimmune diseases ... we conclude that there is no increase in the frequency of antinuclear antibodies in the early phase of Borrelia infection. ...
This study suggests the Borrelia burgdorferi infection is in Mexico. ... Molecular evidence for Borrelia infection. A) PCR for fla gene from Borrelia burgdorferi sensu lato; B) Southern blot assay ... Molecular evidence for Borrelia infection. A) PCR for fla gene from Borrelia burgdorferi sensu lato; B) Southern blot assay ... Serological evidences suggesting the presence of Borrelia burgdorferi infection in Mexico. Arch Med Res. 1999;30:64-8. DOI ...
... revealed infections in dusky-footed woodrats, brush mice, and California mice. Phylogenetic analyses suggest a single, well- ... Surveillance to investigate the wildlife-vector transmission cycle of the human pathogen Borrelia miyamotoi in California, USA ... Krause PJ, Narasimhan S, Wormser GP, Rollend L, Fikrig E, Lepore T, et al. Human Borrelia miyamotoi infection in the United ... Krause PJ, Carroll M, Fedorova N, Brancato J, Dumouchel C, Akosa F, et al. Human Borrelia miyamotoi infection in California: ...
PCR in laboratory diagnosis of human Borrelia burgdorferi infections. Message Subject (Your Name) has forwarded a page to you ... PCR in laboratory diagnosis of human Borrelia burgdorferi infections.. B L Schmidt ... Direct demonstration of Borrelia burgdorferi by culture may require weeks, while enzyme-linked immunosorbent assays for antigen ... This review is restricted to applications of PCR methods in the diagnosis of human B. burgdorferi infections. In the first ...
... organisms in rabbit skin were assessed by real-time PCR over the first 3 weeks of infection. Maximal numbers were found at day ... specific antisera or by matching protein spots to mass spectrometry-identified protein spots from in vitro-cultivated Borrelia ... The numbers of host-adapted Borrelia burgdorferi (HAB) ... antigenic composition of Borrelia burgdorferi during infection ... The numbers of host-adapted Borrelia burgdorferi (HAB) organisms in rabbit skin were assessed by real-time PCR over the first 3 ...
B. miyamatoi infection should now be considered among differential diagnoses for patients from the midwestern United States who ... We confirmed Borrelia miyamotoi infection in 7 patients who had contracted an illness while near La Crosse, Wisconsin, USA, an ... Sato K, Takano A, Konnai S, Nakao M, Ito T, Koyama K, Human infections with Borrelia miyamotoi, Japan. Emerg Infect Dis. 2014; ... Narasimhan S, Wormser GP, Rollend L, Fikrig E, Lepore T, Human Borrelia miyamotoi infection in the United States. N Engl J Med ...
We conducted an investigation of Borrelia miyamotoi infections in humans and ticks in northeastern China. Of 984 patients ... Borrelia miyamotoi Infections in Humans and Ticks, Northeastern China Bao-Gui Jiang1, Na Jia1, Jia-Fu Jiang1, Yuan-Chun Zheng1 ... Epidemiologic, clinical, and laboratory testing features of 14 patients with Borrelia miyamotoi infections, northeastern China ... Borrelia miyamotoi Infections in Humans and Ticks, Northeastern China. ...
Mixed infection of different Borrelia species among Apodemus speciosus mice in Hokkaido, Japan. J Clin Microbiol. 1995;33:490-2 ... Simultaneous Infection of Ixodes ricinus Nymphs by Two Borrelia burgdorferi Sensu Lato Species: Possible Implications for ... Simultaneous Infection of Ixodes ricinus Nymphs by Two Borrelia burgdorferi Sensu Lato Species: Possible Implications for ... Simultaneous Infection of Ixodes ricinus Nymphs by Two Borrelia burgdorferi Sensu Lato Species: Possible Implications for ...
We conducted an investigation of Borrelia miyamotoi infections in humans and ticks in northeastern China. Of 984 patients ... Sato K, Takano A, Konnai S, Nakao M, Ito T, Koyama K, et al. Human infections with Borrelia miyamotoi, Japan. Emerg Infect Dis ... Krause PJ, Narasimhan S, Wormser GP, Rollend L, Fikrig E, Lepore T, et al. Human Borrelia miyamotoi infection in the United ... Borrelia miyamotoi infection in nature and in humans. Clin Microbiol Infect. 2015;21:631-9. DOIPubMed ...
... revealed infections in dusky-footed woodrats, brush mice, and California mice. Phylogenetic analyses suggest a single, well- ... Surveillance to investigate the wildlife-vector transmission cycle of the human pathogen Borrelia miyamotoi in California, USA ... Borrelia miyamotoi Infections in Small Mammals, California, USA Daniel J. Salkeld1. , Nathan C. Nieto1, Denise L. Bonilla2, ... Borrelia miyamotoi Infections in Small Mammals, California, USA. ... Numbers of mammals captured and tested for Borrelia spp., by ...
Culture and sequencing of spirochetes from peripheral blood diagnosed Borrelia turicatae infection. The patient recovered after ... Spirochetemia caused by Borrelia turicatae infection in 3 dogs in Texas. Vet Clin Pathol. 2007;36:212-6. DOIPubMed ... Diagnosis and Management of Borrelia turicatae Infection in Febrile Soldier, Texas, USA On This Page ... Diagnosis and Management of Borrelia turicatae Infection in Febrile Soldier, Texas, USA. Emerging Infectious Diseases. 2017;23( ...
  • Individual proteins were identified using either specific antisera or by matching protein spots to mass spectrometry-identified protein spots from in vitro-cultivated Borrelia. (prohealth.com)
  • Distinct regulatory pathways control expression of Borrelia burgdorferi infection-associated OspC and Erp surface proteins. (prohealth.com)
  • Antibodies to the outer surface proteins (Osps) A, B, and C of the spirochete Borrelia burgdorferi can prevent infection in animal models of Lyme borreliosis. (asm.org)
  • Previously, we had identified non-OspA-OspB surface proteins of Borrelia burgdorferi that are targeted by the antibody-dependent complement-mediated killing mechanism. (asm.org)
  • In a further characterization, Western blots were probed with rabbit antisera raised against either of two purified recombinant Bdr proteins from Borrelia burgdorferi B31. (asm.org)
  • The expression of antigenically distinct variable-surface proteins allows relapsing fever Borrelia to evade host defenses and repopulate the bloodstream ( 63 , 69 ). (asm.org)
  • The dominant proteins (41, 39, and 20 to 24 kDa) recognized by antibody in immune sera during early infection are presumed to be flagellin, P39 (BmpA), and OspC, since antisera to recombinant forms of these proteins react in parallel on immunoblots with immune sera, and immune sera are reactive to these recombinant proteins ( 7 , 10 ). (asm.org)
  • In our quest to link infectivity to any of the plasmids, we determined, along with others, that two linear plasmids, 25 and 28 kb in size (designated lp25 and lp28-1, respectively), were required for maximal infectivity in the mouse model of infection, suggesting that these plasmids encode proteins important for survival of B. burgdorferi in the mammalian host ( 17 , 27 ). (asm.org)
  • Heterogeneity of outer membrane proteins in Borrelia burgdorferi: comparison of osp operons of three isolates of different geographic origins. (asm.org)
  • Previous reports indicated a correlation between loss of plasmids and decreased infectivity of Borrelia burgdorferi strain B31, suggesting that plasmids may encode proteins that are required for pathogenesis. (asm.org)
  • The adenine-thymine rich hairpin loops of the Borrelia bacteria range in size from 5 kilobase pairs (kb) to over 200 kb and contain the genes responsible for producing a group of major surface proteins, or antigens, on the bacteria that allow it to evade the immune response of its infected host. (wikipedia.org)
  • The aim of the present study was to analyze and compare Borrelia strains isolated from two different specimens obtained simultaneously from individual patients with Lyme borreliosis. (asm.org)
  • Analysis of borrelial chromosomal or plasmid DNA, as performed by genotypic methods, provides more precise information on the genetic relationships among Borrelia strains ( 2 , 5 , 7 , 22 , 33 - 35 , 42 , 47 ). (asm.org)
  • Borrelia strains and growth conditions. (asm.org)
  • Consistent with this notion, the terminal complement components (C5b, C6, C7, C8, and C9) are deposited more efficiently and abundantly on the surface of serum-sensitive strains of Borrelia spp. (asm.org)
  • Lice that feed on infected humans acquire the Borrelia organisms that then multiply in the gut of the louse. (wikipedia.org)
  • Global prevalence or incidence of coinfection among humans is unknown, but it is thought to be commonplace, sometimes more common than single infection. (wikipedia.org)
  • Cat fleas can transmit other parasites and infections to dogs and cats and also to humans. (wikipedia.org)
  • Y. enterocolitica infection causes the disease yersiniosis, which is an animal-borne disease occurring in humans, as well as in a wide array of animals such as cattle, deer, pigs, and birds. (wikipedia.org)
  • Acute Y. enterocolitica infections usually lead to mild self-limiting enterocolitis or terminal ileitis and adenitis in humans. (wikipedia.org)
  • As lp's and the chromosome appear to have exchanged genetic information ( 21 ) and some of the cp's carry genes usually found on chromosomes of other prokaryotes ( 36 ), Borrelia plasmids have been likened to minichromosomes ( 6 ). (asm.org)
  • In this study, we characterized seven members of the cp32/18 family of supercoiled plasmids in Borrelia burgdorferi 297. (asm.org)
  • The loss of these plasmids either eliminates infectivity (lp25) or significantly increases the 50% infective dose during a 2-week infection period (lp28-1). (asm.org)
  • In aim 1 we will focus on two Borrelia plasmids (lp25 and lp28-4) required for tick infection. (grantome.com)
  • Infected mice develop a reliable inflammatory arthritis of the ankle joint with severity that typically peaks around two to three weeks post-infection and then undergoes spontaneous resolution. (medworm.com)
  • Borrelia burgdorferi expression of the bba64, bba65, bba66, and bba73 genes in tissues during persistent infection in mice. (nih.gov)
  • On days 21 or 28 after infection (AI) larval I. dammini were fed on the deer mice. (ajtmh.org)
  • The maintenance of Borrelia burgdorferi in a population of Peromyscus leucopus was investigated from 202 mark and recapture mice and 61 mice that were removed from a site in Baltimore County, Maryland. (ajtmh.org)
  • Recovery from infection with B. burgdorferi was not detected among 77 mice followed for an average of 160 days. (ajtmh.org)
  • We have previously demonstrated that immune serum from mice infected with B. burgdorferi N40 can also prevent challenge infection and induce disease regression in infected mice. (asm.org)
  • In both active and passive immunization studies, Osp C (N40) antiserum failed to protect mice from challenge infection with cultured organisms. (asm.org)
  • OspA and OspB were not found in the tissues of SCID mice 17 days after infection. (asm.org)
  • Although mice develop acute arthritis in response to Bb infection, the joint inflammation clears after 2 wk, despite continuous infection, only very rarely presenting with chronic Lyme arthritis. (prohealth.com)
  • Recombinant IL-4 treatment augments resistance to Borrelia burgdorferi infections in both normal susceptible and antibody-deficient susceptible mice. (prohealth.com)
  • We also show that the inherent susceptibility of Ab-deficient, C57BL/6-IgM knockout (B6-MKO) mice to Rh infection is intermediate relative to C57BL/6 severe combined immunodeficient (B6-SCID) mice (susceptible) or normal C57BL/6 mice (resistant), confirming the importance of both Ab-dependent and Ab-independent, T cell-dependent immune mechanisms in control of Bb infections. (prohealth.com)
  • Therefore, MyD88 −/− mice provided a unique opportunity to study the effect of nonantibody, innate host defenses to relapsing fever Borrelia . (asm.org)
  • however, by 6 days of infection antibodies were produced in MyD88 −/− mice that could clear spirochetemia in scid mice. (asm.org)
  • While these results indicated that the production of IgM was delayed in MyD88 −/− mice, they also point to a second, antibody-independent role for MyD88 signaling in host defense to relapsing fever Borrelia . (asm.org)
  • Immunoglobulin M (IgM) antibody plays a critical role in the host defense to relapsing fever Borrelia , as indicated by studies using scid , RAG −/− , B-cell-deficient, and surface IgM −/− mice ( 4 , 18 , 21 ). (asm.org)
  • The production of antibodies and clearance of relapsing fever Borrelia notably occurs in the absence of T cells, as demonstrated in thymectomized mice and T-cell receptor −/− mice ( 4 , 53 ). (asm.org)
  • During an experimental infection in mice, variable regions within the vlsE cassette undergo recombination with the vls silent cassettes via a gene conversion mechanism ( 32 ). (asm.org)
  • A previous study in this laboratory demonstrated that active immunization with a recombinant form of OspC protected mice against a challenge infection administered by tick bite ( 7 ). (asm.org)
  • It was also observed in that study that other mice remained unprotected from the challenge infection even though they harbored OspC antibodies. (asm.org)
  • Our previous studies determined that infected interlukin-10 −/− (IL-10 −/− ) mice show significantly lower B. burgdorferi levels than wild-type (B6) mice and that IL-10 inhibits innate immune responses critical for controlling B. burgdorferi infection. (asm.org)
  • OspA/B are essential for colonization of the tick midgut but are not necessary for infection of mice ( 44 ). (asm.org)
  • A microarray analysis of 137 lipoprotein genes revealed that many additional genes in addition to ospC became downregulated in immunocompetent mice by several weeks after infection, but not in SCID mice ( 26 ). (asm.org)
  • Ehrlichia -infected I. scapularis nymphs were fed upon Borrelia -infected mice, and Borrelia -infected I. scapularis nymphs were fed upon Ehrlichia -infected mice. (asm.org)
  • Further experiments showed the bba64 mutant was not culturable from mouse skin taken at the nymphal bite site and was unable to establish infection in MyD88-deficient mice via tick infestation. (cdc.gov)
  • The population dynamics of Borrelia burgdorferi were quantified by real-time PCR targeting the flaB gene in skin (inoculation site, noninoculation site, and ear), heart (heart base and ventricle), quadriceps muscle, and the tibiotarsal joint at 1, 2, 4, 6, and 8 weeks after intradermal inoculation in C3H and C3H-scid mice. (elsevier.com)
  • Gene transcription was likewise higher in C3H-scid mice than in C3H mice, particularly at 4 or more weeks of infection. (elsevier.com)
  • 1991) Kinetics of Borrelia burgdorferi dissemination and evolution of disease after intradermal inoculation of mice. (wikipedia.org)
  • 2004) Borrelia burgdorferi infection in a natural population of Peromyscus leucopus mice: a longitudinal study in an area where Lyme borreliosis is highly endemic. (wikipedia.org)
  • 1988) The urinary bladder, a consistent source of Borrelia burgdorferi in experimentally infected white-footed mice ( Peromyscus leucopus). (wikipedia.org)
  • However, little is known regarding the identity and/or function of B. burgdorferi genes expressed during colonization of tissues during mammalian infection. (nih.gov)
  • Experimental evidence has shown that a group of genes (formerly classified as paralogous gene family 54) contiguously localized on the 54-kilobase linear plasmid of B. burgdorferi, are among the most highly regulated by in vitro conditions resembling mammalian infection. (nih.gov)
  • Borrelia burgdorferi has developed efficient mechanisms for evading the innate immune response during mammalian infection and has been shown to be resistant to the complement-mediated bactericidal activity of human serum. (asm.org)
  • It has been suggested by several labs, including our own, that B. burgdorferi binds FH/FHL-1 on its surface to inhibit the activation of the alternative pathway of complement and prevent its destruction during the earliest stages of mammalian infection ( 4 , 10 , 40 , 48 , 71 ). (asm.org)
  • however, the IgM response to persistent infection is essentially normal. (asm.org)
  • One of the key topics of debate is the existence of persistent infection with the Lyme spirochete, Borrelia burgdorferi , in patients who have been treated with recommended doses of antibiotics yet remain symptomatic. (preprints.org)
  • He believed Borrelia burgdorferi was a persistent infection, and that the current serological testing methodologies needed to be "started over from scratch, with people who don't know beforehand the results of their research. (wikipedia.org)
  • However, the assumption that there is a persistent infection is not supported by high quality clinical evidence, and the use of long term antibiotics is not recommended. (wikipedia.org)
  • The currently accepted model explaining the infection cycle holds that the transmitting vectors are blood-sucking arthropods and the reservoir hosts are mammals. (wikipedia.org)
  • Therefore, infection should be avoided by preventing arthropod vectors from feeding on the blood of their preferred hosts. (wikipedia.org)
  • This unit provides a chronological in‐depth description of all protocols needed for quantitative reverse transcription-PCR (Q‐RT‐PCR) analysis of Borrelia burgdorferi gene expression within infected mouse tissues. (currentprotocols.com)
  • The presence of B. miyamotoi in the patient's blood was confirmed by PCR, and phylogenetic analysis identified an infection with a strain from Europe. (cdc.gov)
  • In some cases, amplified DNA products were digested with specific restriction enzymes to confirm the typing of the Borrelia strain. (cdc.gov)