Bordetella pertussis. Medical search

A species of gram-negative, aerobic bacteria that is the causative agent of WHOOPING COUGH. Its cells are minute coccobacilli that are surrounded by a slime sheath.

A genus of gram-negative, aerobic bacteria whose cells are minute coccobacilli. It consists of both parasitic and pathogenic species.

A suspension of killed Bordetella pertussis organisms, used for immunization against pertussis (WHOOPING COUGH). It is generally used in a mixture with diphtheria and tetanus toxoids (DTP). There is an acellular pertussis vaccine prepared from the purified antigenic components of Bordetella pertussis, which causes fewer adverse reactions than whole-cell vaccine and, like the whole-cell vaccine, is generally used in a mixture with diphtheria and tetanus toxoids. (From Dorland, 28th ed)

Infections with bacteria of the genus BORDETELLA.

A species of BORDETELLA that is parasitic and pathogenic. It is found in the respiratory tract of domestic and wild mammalian animals and can be transmitted from animals to man. It is a common cause of bronchopneumonia in lower animals.

A respiratory infection caused by BORDETELLA PERTUSSIS and characterized by paroxysmal coughing ending in a prolonged crowing intake of breath.

A set of BACTERIAL ADHESINS and TOXINS, BIOLOGICAL produced by BORDETELLA organisms that determine the pathogenesis of BORDETELLA INFECTIONS, such as WHOOPING COUGH. They include filamentous hemagglutinin; FIMBRIAE PROTEINS; pertactin; PERTUSSIS TOXIN; ADENYLATE CYCLASE TOXIN; dermonecrotic toxin; tracheal cytotoxin; Bordetella LIPOPOLYSACCHARIDES; and tracheal colonization factor.

One of the virulence factors produced by BORDETELLA PERTUSSIS. It is a multimeric protein composed of five subunits S1 - S5. S1 contains mono ADPribose transferase activity.

A species of BORDETELLA with similar morphology to BORDETELLA PERTUSSIS, but growth is more rapid. It is found only in the RESPIRATORY TRACT of humans.

Agents that cause agglutination of red blood cells. They include antibodies, blood group antigens, lectins, autoimmune factors, bacterial, viral, or parasitic blood agglutinins, etc.

One of the virulence factors produced by virulent BORDETELLA organisms. It is a bifunctional protein with both ADENYLYL CYCLASES and hemolysin components.

A species of BORDETELLA isolated from the respiratory tracts of TURKEYS and other BIRDS. It causes a highly contagious bordetellosis.

Vaccines that are produced by using only the antigenic part of the disease causing organism. They often require a "booster" every few years to maintain their effectiveness.

Immunoglobulins produced in a response to BACTERIAL ANTIGENS.

Cell-surface components or appendages of bacteria that facilitate adhesion (BACTERIAL ADHESION) to other cells or to inanimate surfaces. Most fimbriae (FIMBRIAE, BACTERIAL) of gram-negative bacteria function as adhesins, but in many cases it is a minor subunit protein at the tip of the fimbriae that is the actual adhesin. In gram-positive bacteria, a protein or polysaccharide surface layer serves as the specific adhesin. What is sometimes called polymeric adhesin (BIOFILMS) is distinct from protein adhesin.

An enzyme of the lyase class that catalyzes the formation of CYCLIC AMP and pyrophosphate from ATP. EC 4.6.1.1.

A transient increase in the number of leukocytes in a body fluid.

Substances, usually of biological origin, that cause cells or other organic particles to aggregate and stick to each other. They include those ANTIBODIES which cause aggregation or agglutination of particulate or insoluble ANTIGENS.

Proteins isolated from the outer membrane of Gram-negative bacteria.

Proteins found in any species of bacterium.

Combined vaccines consisting of DIPHTHERIA TOXOID; TETANUS TOXOID; and an acellular form of PERTUSSIS VACCINE. At least five different purified antigens of B. pertussis have been used in various combinations in these vaccines.

A vaccine consisting of DIPHTHERIA TOXOID; TETANUS TOXOID; and whole-cell PERTUSSIS VACCINE. The vaccine protects against diphtheria, tetanus, and whooping cough.

The top portion of the pharynx situated posterior to the nose and superior to the SOFT PALATE. The nasopharynx is the posterior extension of the nasal cavities and has a respiratory function.

Substances elaborated by bacteria that have antigenic activity.

Preparations of pathogenic organisms or their derivatives made nontoxic and intended for active immunologic prophylaxis. They include deactivated toxins. Anatoxin toxoids are distinct from anatoxins that are TROPANES found in CYANOBACTERIA.

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

The functional hereditary units of BACTERIA.

The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.

Excess of normal lymphocytes in the blood or in any effusion.

Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.

Deoxyribonucleic acid that makes up the genetic material of bacteria.

Administration of vaccines to stimulate the host's immune response. This includes any preparation intended for active immunological prophylaxis.

Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity.

Low-molecular-weight compounds produced by microorganisms that aid in the transport and sequestration of ferric iron. (The Encyclopedia of Molecular Biology, 1994)

The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.

The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.

Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).

The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.

Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (From Stedman, 26th ed)

Thin, hairlike appendages, 1 to 20 microns in length and often occurring in large numbers, present on the cells of gram-negative bacteria, particularly Enterobacteriaceae and Neisseria. Unlike flagella, they do not possess motility, but being protein (pilin) in nature, they possess antigenic and hemagglutinating properties. They are of medical importance because some fimbriae mediate the attachment of bacteria to cells via adhesins (ADHESINS, BACTERIAL). Bacterial fimbriae refer to common pili, to be distinguished from the preferred use of "pili", which is confined to sex pili (PILI, SEX).

The formaldehyde-inactivated toxin of Corynebacterium diphtheriae. It is generally used in mixtures with TETANUS TOXOID and PERTUSSIS VACCINE; (DTP); or with tetanus toxoid alone (DT for pediatric use and Td, which contains 5- to 10-fold less diphtheria toxoid, for other use). Diphtheria toxoid is used for the prevention of diphtheria; DIPHTHERIA ANTITOXIN is for treatment.

The cartilaginous and membranous tube descending from the larynx and branching into the right and left main bronchi.

A chronic inflammation in which the NASAL MUCOSA gradually changes from a functional to a non-functional lining without mucociliary clearance. It is often accompanied by degradation of the bony TURBINATES, and the foul-smelling mucus which forms a greenish crust (ozena).

Regulatory proteins that act as molecular switches. They control a wide range of biological processes including: receptor signaling, intracellular signal transduction pathways, and protein synthesis. Their activity is regulated by factors that control their ability to bind to and hydrolyze GTP to GDP. EC 3.6.1.-.

Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.

An ester formed between the aldehydic carbon of RIBOSE and the terminal phosphate of ADENOSINE DIPHOSPHATE. It is produced by the hydrolysis of nicotinamide-adenine dinucleotide (NAD) by a variety of enzymes, some of which transfer an ADP-ribosyl group to target proteins.

The tubular and cavernous organs and structures, by means of which pulmonary ventilation and gas exchange between ambient air and the blood are brought about.

Suspensions of attenuated or killed bacteria administered for the prevention or treatment of infectious bacterial disease.

Proteins from BACTERIA and FUNGI that are soluble enough to be secreted to target ERYTHROCYTES and insert into the membrane to form beta-barrel pores. Biosynthesis may be regulated by HEMOLYSIN FACTORS.

Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.

Antisera from immunized animals that is purified and used as a passive immunizing agent against specific BACTERIAL TOXINS.

An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.

In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.

An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.

Represents 15-20% of the human serum immunoglobulins, mostly as the 4-chain polymer in humans or dimer in other mammals. Secretory IgA (IMMUNOGLOBULIN A, SECRETORY) is the main immunoglobulin in secretions.

Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.

Role of antibodies against Bordetella pertussis virulence factors in adherence of Bordetella pertussis and Bordetella parapertussis to human bronchial epithelial cells. (1/1244)

Immunization with whole-cell pertussis vaccines (WCV) containing heat-killed Bordetella pertussis cells and with acellular vaccines containing genetically or chemically detoxified pertussis toxin (PT) in combination with filamentous hemagglutinin (FHA), pertactin (Prn), or fimbriae confers protection in humans and animals against B. pertussis infection. In an earlier study we demonstrated that FHA is involved in the adherence of these bacteria to human bronchial epithelial cells. In the present study we investigated whether mouse antibodies directed against B. pertussis FHA, PTg, Prn, and fimbriae, or against two other surface molecules, lipopolysaccharide (LPS) and the 40-kDa outer membrane porin protein (OMP), that are not involved in bacterial adherence, were able to block adherence of B. pertussis and B. parapertussis to human bronchial epithelial cells. All antibodies studied inhibited the adherence of B. pertussis to these epithelial cells and were equally effective in this respect. Only antibodies against LPS and 40-kDa OMP affected the adherence of B. parapertussis to epithelial cells. We conclude that antibodies which recognize surface structures on B. pertussis or on B. parapertussis can inhibit adherence of the bacteria to bronchial epithelial cells, irrespective whether these structures play a role in adherence of the bacteria to these cells. (+info)

Role of Bordetella pertussis virulence factors in adherence to epithelial cell lines derived from the human respiratory tract. (2/1244)

During colonization of the respiratory tract by Bordetella pertussis, virulence factors contribute to adherence of the bacterium to the respiratory tract epithelium. In the present study, we examined the roles of the virulence factors filamentous hemagglutinin (FHA), fimbriae, pertactin (Prn), and pertussis toxin (PT) in the adherence of B. pertussis to cells of the human bronchial epithelial cell line NCI-H292 and of the laryngeal epithelial cell line HEp-2. Using B. pertussis mutant strains and purified FHA, fimbriae, Prn, and PT, we demonstrated that both fimbriae and FHA are involved in the adhesion of B. pertussis to laryngeal epithelial cells, whereas only FHA is involved in the adherence to bronchial epithelial cells. For PT and Prn, no role as adhesion factor was found. However, purified PT bound to both bronchial and laryngeal cells and as such reduced the adherence of B. pertussis to these cells. These data may imply that fimbriae play a role in infection of only the laryngeal mucosa, while FHA is the major factor in colonization of the entire respiratory tract. (+info)

Characterization of human bactericidal antibodies to Bordetella pertussis. (3/1244)

The Bordetella pertussis BrkA protein protects against the bactericidal activity of complement and antibody; however, some individuals mount an immune response that overcomes this bacterial defense. To further characterize this process, the bactericidal activities of sera from 13 adults with different modes of exposure to B. pertussis (infected as adults, occupational exposure, immunized with an acellular vaccine, or no identified exposure) against a wild-type strain and a BrkA complement-sensitive mutant were evaluated. All of the sera killed the BrkA mutant, suggesting past exposure to B. pertussis or cross-reactive organisms. Several samples had no or minimal activity against the wild type. All of the sera collected from the infected and occupationally exposed individuals but not all of the sera from vaccinated individuals had bactericidal activity against the wild-type strain, suggesting that some types of exposure can induce an immune response that can overcome the BrkA resistance mechanism. Adsorbing serum with the wild-type strain removed the bactericidal antibodies; however, adsorbing the serum with a lipopolysaccharide (LPS) mutant or an avirulent (bvg mutant) strain did not always result in loss of bactericidal activity, suggesting that antibodies to either LPS or bvg-regulated proteins could be bactericidal. All the samples, including those that lacked bactericidal activity, contained antibodies that recognized the LPS of B. pertussis. Bactericidal activity correlated best with the presence of the immunoglobulin G3 (IgG3) antibodies to LPS, the IgG subtype that is most effective at fixing complement. (+info)

Maternal immunization. (4/1244)

Maternal immunization can enhance passive immunity of infants to pathogens that cause life-threatening illnesses. In most instances, immunization during pregnancy will provide important protection for the woman as well as for her offspring. The tetanus toxoid and influenza vaccines are examples of vaccines that provide a double benefit. Other vaccines under evaluation include those for respiratory syncytial virus, pneumococci, group B streptococci, and Haemophilus influenzae type b. Although most IgG antibody crosses the placenta in the third trimester, the process is time-dependent, dictating that immunization should be accomplished ideally at least 6 weeks prior to delivery. IgG1 antibodies are transferred preferentially. Maternal immunization has not interfered with active immunization of the infant. Inactivated vaccines administered in the third trimester of pregnancy pose no known risk to the woman or to her fetus. (+info)

Temporal trends in the population structure of Bordetella pertussis during 1949-1996 in a highly vaccinated population. (5/1244)

The population structure of Bordetella pertussis in The Netherlands in 5 successive periods, encompassing 1949-1996, was analyzed by DNA typing ("fingerprinting"). In 10 years following the introduction of wide-scale vaccination in 1953, a decrease in genotypic diversity (GD) was observed, suggesting clonal expansion of strains that were adapted to vaccine-induced immunity. In subsequent periods, GD increased to prevaccination levels, probably reflecting a gradual adaptation of the B. pertussis population involving many lineages. In the 1990s, GD decreased again. This decrease coincided with an antigenic shift in the surface protein pertactin. No evidence was found for changes in DNA types or GD in 1996, when a large pertussis epidemic occurred. Thus, gradual changes in the bacterial population previous to 1996 were probably the cause of the 1996 epidemic. The results herein suggest that vaccination has selected for strains that are adapted to a highly vaccinated population. Similar changes may have occurred in other countries, explaining the reemergence of pertussis in vaccinated populations. (+info)

Analysis with a combination of macrorestriction endonucleases reveals a high degree of polymorphism among Bordetella pertussis isolates in eastern France. (6/1244)

From 1990 to 1996, routine screening for whooping cough identified 399 patients with a calmodulin-dependent adenylate cyclase-positive test result and yielded 69 Bordetella pertussis isolates. None of the patients were fully vaccinated, and most were less than 6 months old. Analysis of total DNA by pulsed-field gel electrophoresis (PFGE) after XbaI, SpeI, or DraI macrorestriction yielded 19, 15, and 5 different patterns, respectively, whereas ribotyping failed to demonstrate any strain polymorphism. Discrimination among the isolates was improved by combining the PFGE profiles. Some patterns were more frequent, but the corresponding patients were not clearly epidemiologically related. The patterns for two strains obtained during a 3-month period from patients who were neighbors differed by the length of a single DNA fragment. These data strongly suggest that one type of isolate is widely spread throughout the world and is carried by individuals other than patients who develop a true illness. (+info)

Serum IgG antibody responses to pertussis toxin and filamentous hemagglutinin in nonvaccinated and vaccinated children and adults with pertussis. (7/1244)

Levels of IgG antibody to pertussis toxin (PT) and filamentous hemagglutinin (FHA) were measured in paired serum samples from 781 patients fulfilling at least one laboratory criterion for pertussis that was suggested by an ad hoc committee sponsored by the World Health Organization. The patients were participants or family members of participants in a double-blind efficacy trial of a monocomponent pertussis toxoid vaccine. Of 596 nonvaccinated children, 90% had significant (two-fold or more) rises in PT IgG and FHA IgG levels. Only 17 (32%) of 53 children previously vaccinated with three doses of pertussis toxoid had rises in PT IgG levels because they already had elevated PT IgG levels in their acute-phase serum samples. PT IgG and FHA IgG levels were significantly higher in acute-phase serum samples from 29 adults than in acute-phase serum samples from the nonvaccinated children. Nevertheless, significant rises in levels of PT IgG (79% of samples) and FHA IgG (90%) were demonstrated in adults. In conclusion, assay of PT IgG and FHA IgG in paired serum samples is highly sensitive for diagnosing pertussis in nonvaccinated individuals. Assay of PT IgG levels in paired sera is significantly less sensitive for diagnosis of pertussis for children vaccinated with pertussis toxoid. (+info)

The conserved lysine 860 in the additional fatty-acylation site of Bordetella pertussis adenylate cyclase is crucial for toxin function independently of its acylation status. (8/1244)

The Bordetella pertussis RTX (repeat in toxin family protein) adenylate cyclase toxin-hemolysin (ACT) acquires biological activity upon a single amide-linked palmitoylation of the epsilon-amino group of lysine 983 (Lys983) by the accessory fatty-acyltransferase CyaC. However, an additional conserved RTX acylation site can be identified in ACT at lysine 860 (Lys860), and this residue becomes palmitoylated when recombinant ACT (r-Ec-ACT) is produced together with CyaC in Escherichia coli K12. We have eliminated this additional acylation site by replacing Lys860 of ACT with arginine, leucine, and cysteine residues. Two-dimensional gel electrophoresis and microcapillary high performance liquid chromatography/tandem mass spectrometric analyses of mutant proteins confirmed that the two sites are acylated independently in vivo and that mutations of Lys860 did not affect the quantitative acylation of Lys983 by palmitoyl (C16:0) and palmitoleil (cis Delta9 C16:1) fatty-acyl groups. Nevertheless, even the most conservative substitution of lysine 860 by an arginine residue caused a 10-fold decrease of toxin activity. This resulted from a 5-fold reduction of cell association capacity and a further 2-fold reduction in cell penetration efficiency of the membrane-bound K860R toxin. These results suggest that lysine 860 plays by itself a crucial structural role in membrane insertion and translocation of the toxin, independently of its acylation status. (+info)

Pertussis (Whooping cough) Bordetella pertussis Plague Yersinia pestis Pneumococcal infection Streptococcus pneumoniae ...

https://en.wikipedia.org/wiki/List_of_infectious_diseases

Bordetella pertussis 1. She was a full professor of biomedicine at Mucosal Immunology and Vaccine Center in Gothenburg. ...

https://en.wikipedia.org/wiki/Teresa_Lagergård

... is an adenylate cyclase produced by Bordetella pertussis. Kessin RH, Franke J (April 1986). " ... Ladant D, Brezin C, Alonso JM, Crenon I, Guiso N (December 1986). "Bordetella pertussis adenylate cyclase. Purification, ... "Secreted adenylate cyclase of Bordetella pertussis: calmodulin requirements and partial purification of two forms". J. ...

https://en.wikipedia.org/wiki/Extracellular_adenylate_cyclase

In Bordetella pertussis, the infectious agent in childhood whooping cough, filamentous haemagglutinin (FHA) is a surface- ... "Filamentous hemagglutinin of Bordetella pertussis. A bacterial adhesin formed as a 50-nm monomeric rigid rod based on a 19- ... "Beta-helix model for the filamentous haemagglutinin adhesin of Bordetella pertussis and related bacterial secretory proteins". ... Inatsuka CS, Julio SM, Cotter PA (December 2005). "Bordetella filamentous hemagglutinin plays a critical role in ...

https://en.wikipedia.org/wiki/Haemagglutination_activity_domain

Diseases such as pertussis (or whooping cough) are caused by the bacteria Bordetella pertussis. This bacteria is marked by a ... Yeh, Sylvia H.; Mink, ChrisAnna M. (2012). "Bordetella pertussis and Pertussis (Whooping Cough)". Netter's Infectious Diseases ... The pertussis toxin is a protein exotoxin that binds to cell receptors by two dimers and reacts with different cell types such ... PCR is an important testing tool that can detect sequences within the gene for the pertussis toxin. Because PCR has a high ...

https://en.wikipedia.org/wiki/Polymerase_chain_reaction

2003). "Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella ... and Clinical Manifestations of Respiratory Infections Due to Bordetella pertussis and Other Bordetella Subspecies". Clinical ... 1991). "Effects of Bordetella pertussis infection on human respiratory epithelium in vivo and in vitro". Infection and Immunity ... Luker, K. E.; Collier, J. L.; Kolodziej, E. W.; Marshall, G. R.; Goldman, W. E. (1993). "Bordetella pertussis Tracheal ...

https://en.wikipedia.org/wiki/Tracheal_cytotoxin

Corynebacterium diphtheriae and Bordetella pertussis. ubiquitination and SUMOylation Various full-length, folded proteins can ...

https://en.wikipedia.org/wiki/Protein_primary_structure

Pertussis is caused by the bacterium Bordetella pertussis. It is spread easily through the coughs and sneezes of an infected ... Pertussis is caused by the bacterium Bordetella pertussis. It is an airborne disease (through droplets) that spreads easily ... Pertussis infection in these persons may be asymptomatic, or present as illness ranging from a mild cough to classic pertussis ... The pertussis vaccine became available in the 1940s. Play media The classic symptoms of pertussis are a paroxysmal cough, ...

https://en.wikipedia.org/wiki/Whooping_cough

Pertussis toxin is secreted by the gram-negative bacterium, Bordetella pertussis. Whooping cough is very contagious and cases ... Guiso N. 2009.Bordetella pertussis and pertussis vaccines. Clin. Infect. Dis. 49:1565-1569 Faruque, S. M.; Chowdhury, N; Khan, ... Carbonetti, N. H. (2010). "Pertussis toxin and adenylate cyclase toxin: Key virulence factors of Bordetella pertussis and cell ... The bacterium Bordetella pertussis was first identified as the cause of whooping cough and isolated by Jules Bordet and Octave ...

https://en.wikipedia.org/wiki/AB5_toxin

With Jules Bordet he isolated Bordetella pertussis in pure culture in 1906 and declared it as the cause of whooping cough. In ... He researched with Jules Bordet the Bordetella pertussis bacteria. At the age of 22, he obtained his doctorate at the ... The pertussis endotoxin. Etiologie de la coqueluche. Etat actuel de la question, 1909 - Etiology of pertussis. Current status ... Le Microbe de la coqueluche, 1906 - The microbe "pertussis". Note complémentaire sur le microbe de la coqueluche, 1906 - ...

https://en.wikipedia.org/wiki/Octave_Gengou

... is a highly immunogenic virulence factor of Bordetella pertussis, the bacterium that causes pertussis. Specifically, it is an ... PRN is purified from Bordetella pertussis and is used for the vaccine production as one of the important components of ... Emsley, P.; Charles, I. G.; Fairweather, N. F.; Isaacs, N. W. (1996). "Structure of Bordetella pertussis virulence factor P.69 ... Emsley P, Charles IG, Fairweather NF, Isaacs NW (May 1996). "Structure of Bordetella pertussis virulence factor P.69 pertactin ...

https://en.wikipedia.org/wiki/Pertactin

Bordetella pertussis. Bordetella bronchiseptica and Bordetella parapertussis, also able to cause pertussis-like symptoms, also ... Vaccination against Bordetella pertussis is used in infancy to prevent whooping cough. The recent switch from whole-cell ... Adenylate cyclase toxin is a virulence factor produced by some members of the genus Bordetella. Together with the pertussis ... Adenylate cyclase toxin from Bordetella pertussis is a 1706 amino acid residue long protein. The protein consists of three ...

https://en.wikipedia.org/wiki/Adenylate_cyclase_toxin

"Vaccine Development for the Control of Bordetella Pertussis Infections". Infection and Immunity. 86 (6): e00004-18. doi:10.1128 ...

https://en.wikipedia.org/wiki/List_Biological_Laboratories

"Interaction of human Tamm-Horsfall glycoprotein with Bordetella pertussis toxin". Microbiology. 148 (Pt 4): 1193-1201. doi: ...

https://en.wikipedia.org/wiki/Uromodulin

Bordetella pertussis, Haemophilus influenzae), and some respiratory tract and soft-tissue infections. The antimicrobial ... "Infantile hypertrophic pyloric stenosis after pertussis prophylaxis with erythromcyin: a case review and cohort study". Lancet ...

https://en.wikipedia.org/wiki/Macrolide

Bordetella pertussis is a Gram-negative coccobacillus responsible for causing whooping cough. Yersinia pestis, the bacteria ...

https://en.wikipedia.org/wiki/Coccobacillus

"Pertussis toxin and adenylate cyclase toxin provide a one-two punch for establishment of Bordetella pertussis infection of the ... "The adenylate cyclase toxin of Bordetella pertussis binds to target cells via the alpha(M)beta(2) integrin (CD11b/CD18)". J. ... "Structural basis for the interaction of Bordetella pertussis adenylyl cyclase toxin with calmodulin". EMBO J. 24 (18): 3190-201 ... Pertussis toxin Anthrax toxin PDB: 2COL; Guo Q, Shen Y, Lee YS, Gibbs CS, Mrksich M, Tang WJ (September 2005). " ...

https://en.wikipedia.org/wiki/CyaA

Gordon's work focused on pertussis cultures and virulence of the bacterium Bordetella pertussis. Gordon's analysis of pertussis ... Kendrick to support pertussis research at the Michigan Department of Health's Grand Rapids lab around 1944. In the early 1940s ... and the Grand Rapids Pertussis Trials, 1932-1939". Michigan Historical Review. 33 (1): 59-85. JSTOR 20174193. "Journal No. 62 ... doctors Pearl Kendrick and Grace Eldering with bacteriological virulence research leading to the creation of the pertussis ...

https://en.wikipedia.org/wiki/Loney_Gordon

Bordetella pertussis) 2014 Case Definition. Centers for Disease Control and Prevention. Retrieved December 16, 2015. CS1 maint ... CS1 maint: discouraged parameter (link) "CSTE Position Statement 13-ID-15". CDC (NNDSS) Diseases and Conditions - Pertussis / ...

https://en.wikipedia.org/wiki/Council_of_State_and_Territorial_Epidemiologists

Skerry C, Goldman WE, Carbonetti NH (February 2019). "Bordetella pertussis and Contributes to Sphingosine-1-Phosphate Receptor ... pneumonia associated with impaired bacterial clearance and more severe pulmonary inflammation following Bordetella pertussis ...

https://en.wikipedia.org/wiki/Peptidoglycan_recognition_protein_4

These adenylyl cyclases are toxins secreted by pathogenic bacteria such as Bacillus anthracis, Bordetella pertussis, ... "Structural basis for the interaction of Bordetella pertussis adenylyl cyclase toxin with calmodulin". The EMBO Journal. 24 (18 ...

https://en.wikipedia.org/wiki/Adenylyl_cyclase

Pertussis is caused by the bacterium Bordetella pertussis.[4] It is an airborne disease which spreads easily through the coughs ... Pertussis is caused by the bacterium Bordetella pertussis. It is an airborne disease (through droplets) that spreads easily ... and Clinical Manifestations of Respiratory Infections Due to Bordetella pertussis and Other Bordetella Subspecies". Clin ... Ebell, MH; Marchello, C; Callahan, M (2017). "Clinical Diagnosis of Bordetella Pertussis Infection: A Systematic Review". J Am ...

https://en.m.wikipedia.org/wiki/Whooping_cough

"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica". ...

https://en.wikipedia.org/wiki/Duncan_Maskell

Schnoeller, C; Roux, X; Sawant, D; Raze, D; Olszewska, W; Locht, C; Openshaw, PJ (2013). "Attenuated Bordetella Pertussis ...

https://en.wikipedia.org/wiki/Peter_Openshaw_(immunologist)

The C-terminal domain of the adenylate cyclase toxin (ACT or CyaA; TC# 1.C.11.1.4) of Bordetella pertussis forms a small cation ... Adenylate cyclase toxin (ACT or CyaA), is a primary virulence factor in Bordetella pertussis. CyaA is a multifunctional RTX ... "Membrane restructuring by Bordetella pertussis adenylate cyclase toxin, a member of the RTX toxin family". Journal of ... "The Adenylate Cyclase Toxin of Bordetella pertussis Binds to Target Cells via the αMβ2 Integrin (Cd11b/Cd18)". Journal of ...

https://en.wikipedia.org/wiki/RTX_toxin

"Mannose receptor and macrophage galactose-type lectin are involved in Bordetella pertussis mast cell interaction". Journal of ...

https://en.wikipedia.org/wiki/Pattern_recognition_receptor

Because pertussis is known to be caused by the bacterium Bordetella pertussis, vaccination is effective. A paper co-authored in ... July 2018). "Pertussis Immunisation in Pregnancy Safety (PIPS) Study: A retrospective cohort study of safety outcomes in ... "Pertussis (Whooping Cough)". Centers for Disease Control and Prevention. Retrieved 20 November 2020. Chisholm, Hannah; Howe, ... In 1916 Petousis-Harris and a team of researchers evaluated whether the switch from whole cell to acellular pertussis vaccine ...

https://en.wikipedia.org/wiki/Helen_Petousis-Harris

2003). "Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella ... and Bordetella. Achtman was one of the inventors of multilocus sequence typing. His research has been funded by the ...

https://en.wikipedia.org/wiki/Mark_Achtman

Bordetella pertussis, the causative bacterium of whooping cough, secretes its pertussis toxin partly through T4SS. Legionella ...

https://en.wikipedia.org/wiki/Bacterial_secretion_system

Bordetella pertussis, Fusobacterium sp., Corynebacterium diphtheriae, Treponema pallidum, and Neisseria gonorrhoeae. Anaerobic ...

https://en.wikipedia.org/wiki/Tonsillitis

Tako kot Bordetella pertussis tudi ta vrsta tvori od kalmodulina odvisni adenilat-ciklazni eksotoksin, imenovan edemski faktor ...

https://sl.wikipedia.org/wiki/Bacillus_anthracis

Bordetella pertussis/Bordetella parapertussis *Pertussis. γ. Enterobacteriales. (OX−). Lac+. *Klebsiella pneumoniae * ...

https://en.wikipedia.org/wiki/Urinary_Tract_Infections

gadā (Bordetella pertussis jeb Bordē-Žangū baktērija).[9] Slimības ierosinātājs ir Bordetella pertussis, nekustīga gramnegatīva ... Infant Pertussis Study Group. Transmission of Bordetella pertussis to young infants. Pediatr Infect Dis J., 2007 Apr;26(4):293- ... 21,0 21,1 21,2 ECDC Guidance and protocol for the serological diagnosis of human infection with Bordetella pertussis. 2012 Oct; ... Polymorphism of Bordetella pertussis Isolates Circulating for the Last 10 Years in France, Where a Single Effective Whole-Cell ...

https://lv.wikipedia.org/wiki/Garais_klepus

Bordetella pertussis. *Bradyrhizobium japonicum. *Burkholderia gladioli. C. *Campylobacter. *Campylobacterales. *Chlamydia ( ...

https://id.wikipedia.org/wiki/Kategori:Rintisan_bertopik_bakteri

Bordetella pertussis/Bordetella parapertussis *Pertussis. γ. Enterobacteriales. (OX−). Lac+. *Klebsiella pneumoniae * ...

https://en.wikipedia.org/wiki/Gonorrhea

Bordetella pertussis/Bordetella parapertussis *Pertussis. γ. Enterobacteriales. (OX−). Lac+. *Klebsiella pneumoniae * ...

https://en.wikipedia.org/wiki/Bacillary_dysentery

Bordetella pertussis, the causative agent of whooping cough, secretes the pertussis toxin partly through the type IV system. ...

https://en.wikipedia.org/wiki/Secrete

... small number of cases are due to high levels of air pollution or bacteria such as Mycoplasma pneumoniae or Bordetella pertussis ... small number of cases are due to high levels of air pollution or bacteria such as Mycoplasma pneumoniae or Bordetella pertussis ... An exception is when acute bronchitis is due to pertussis. Tentative evidence supports honey and pelargonium to help with ...

https://en.wikipedia.org/wiki/Bronchitus

Burkholderia pseudomallei (Melioidosis) · Burkholderia mallei (Glanders) · Burkholderia cepacia complex · Bordetella pertussis/ ...

https://ml.wikipedia.org/wiki/ടൈഫോയ്ഡ്

백일해(百日咳; 문화어: 백날기침; 영어: pertussis)는 전염성이 매우 높은 세균병이다.[1][2][3][4] 백일해균(학명: Bordetella pertussis)으로 일어나는 어린이의 호흡기 전염병으로서, 한번 걸리면 ... Top KA, Halperin SA (2017). 》Pertussis and other Bordetella infections》. Kasper DL, Fauci AS. 》Harrison's Infectious Diseases》 ... Carbonetti NH (June 2007). "Immunomodulation in the pathogenesis of Bordetella pertussis infection and disease". 》Curr Opin ... Carbonetti NH (June 2007). "Immunomodulation in the pathogenesis of Bordetella pertussis infection and disease". 》Current ...

https://ko.wikipedia.org/wiki/백일해

Bordetella pertussis. Whooping cough. DPT vaccine. Boostrix, Adacel, Daptacel, Infanrix, Tripedia, Kinrix, Pediarix, Pentacel, ...

https://en.wikipedia.org/wiki/List_of_vaccine_topics

Bordetella pertussis/Bordetella parapertussis *Pertussis. γ. Enterobacteriales. (OX−). Lac+. *Klebsiella pneumoniae * ...

https://en.wikipedia.org/wiki/Montezuma's_Revenge_

Je to jedna z mála známych baktérií, ktorá dokáže syntetizovať proteínovú kapsulu, Podobne ako Bordetella pertussis, formuje ...

https://sk.wikipedia.org/wiki/Bacillus_anthracis

Bordetella pertussis/Bordetella parapertussis *Pertussis. γ. Enterobacteriales. (OX-). Lac+. *Klebsiella pneumoniae * ...

https://kn.wikipedia.org/wiki/ಜಠರ/ಜಠರೀಯ_ಹುಣ್ಣು/ವ್ರಣ

വില്ലൻചുമയെ പ്രതിരോധിക്കുന്ന വാക്സിൻ ആണ് പെർട്ടുസ്സിസ് വാക്സിൻ(Pertussis vaccine).[1] ഇവ പ്രധാനമായും രണ്ടുതരത്തിലാണുള്ളത്: മുഴു ... Bordetella pertussis. Type. ?. Clinical data. MedlinePlus. a682198. Legal status. Legal status. *℞ (Prescription only) ...

https://ml.wikipedia.org/wiki/Pertussis_vaccine

Bordetella pertussis/Bordetella parapertussis *Pertussis. γ. Enterobacteriales. (OX−). Lac+. *Klebsiella pneumoniae * ...

https://en.wikipedia.org/wiki/Meningococcemia

Bordetella pertussis/Bordetella parapertussis *Pertussis. γ. Enterobacteriales. (OX−). Lac+. *Klebsiella pneumoniae * ...

https://en.wikipedia.org/wiki/Chromobacteriosis_infection

Bordetella pertussis/Bordetella parapertussis *Pertussis. γ. Enterobacteriales. (OX−). Lac+. *Klebsiella pneumoniae * ...

https://en.wikipedia.org/wiki/Timeline_of_plague

Corynebacterium diphtheriae and Bordetella pertussis.. *ubiquitination and SUMOylation. Various full-length, folded proteins ...

https://en.m.wikipedia.org/wiki/Protein_primary_structure

... as well as Bordetella pertussis which causes whooping cough. Other members of the class can infect plants, such as Burkholderia ...

https://en.wikipedia.org/wiki/Betaproteobacteria

Diseases such as pertussis (or whooping cough) are cause by the bacteria Bordetella pertussis. This bacteria is marked by a ... The pertussis toxin is a protein exotoxin that binds to cell receptors by two dimers and reacts with different cell types such ... PCR is very efficient for diagnosing pertussis when compared to culture.[34] ... PCR is an important testing tool that can detect the sequences that are within the pertussis toxin gene. This is because PCR ...

https://en.m.wikipedia.org/wiki/Polymerase_chain_reaction

Pertussis toxin (PTx) of Bordetella pertussis, formerly known as lymphocytosis-promoting factor, causes a decrease in the entry ...

https://en.m.wikipedia.org/wiki/Lymphocytes

Seperti Bordetella pertussis, ia membentuk eksotoksin adenilat siklase yang bergantung pada kalmodulin yang dikenal sebagai ...

https://id.m.wikipedia.org/wiki/Bacillus_anthracis

Burkholderia pseudomallei (Melioidosis) · Burkholderia mallei (Glanders) · Burkholderia cepacia complex · Bordetella pertussis/ ...

https://bn.wikipedia.org/wiki/কলেরা

Bordetella pertussis/Bordetella parapertussis *Pertussis. γ. Enterobacteriales. (OX−). Lac+. *Klebsiella pneumoniae * ...

https://en.wikipedia.org/wiki/List_of_parasites_of_humans

The 'a' in DTaP and Tdap stands for 'acellular,' meaning that the pertussis component contains only a part of the pertussis ... such as Bordetella bronchiseptica, has likely increased in recent years.[104] In some cases, most notably rabies, the parallel ... toxoids and pertussis (P) vaccine. Lower-case "d" and "p" denote reduced doses of diphtheria and pertussis used in the ... In the preparation for the 1990 Persian Gulf campaign, whole cell pertussis vaccine was used as an adjuvant for anthrax vaccine ...

https://en.m.wikipedia.org/wiki/Vaccine

위험 요인으로는 흡연, 먼지 및 기타 대기 오염에 대한 노출이 포함된다.[1] 폐렴미코플라스마(Mycoplasma pneumoniae) 나 백일해균(Bordetella pertussis)과 같은 박테리아나 고농도의 대기 오염에 ... Bordetella pertussis)과 같은 박테리아나 고농도의 대기 오염에 의한 경우도 소량 존재한다.[2][3]급성 기관지염의 치료에는 일반적으로 휴식, paracetamol (acetaminophen) 및 NSAIDs가 ...

https://ko.wikipedia.org/wiki/기관지염

Definition of Bordetella pertussis. Provided by Stedmans medical dictionary and Drugs.com. Includes medical terms and ... Bordetella pertussis. Definition: the bacterial species that is the causative agent of whooping cough, a respiratory tract ... is associated with production of pertussis toxin, a protein consisting of 5 B. subunits that bind the molecule to respiratory ...

https://www.drugs.com/dict/bordetella-pertussis.html

In addition, the B. pertussis ASR contains primers, a Texas Red-labeled probe and DNA for an internal control sequence. This ... pertussis ASR contains primers and a FAM-labeled probe that is designed to detect a 103 bp region of the IS481 gene. ... Bordetella pertussis Antibody, IgM. 5. Bordetella pertussis Antibody, IgG. 6. Bordetella pertussis by PCR. 7. Bordetella ... Bordetella pertussis DFA. 10. Bordetella pertussis Toxin Antibody, IgA. 11. Bordetella pertussis Toxin Antibody, IgG. ...

http://www.bio-medicine.org/medicine-products/Bordetella-pertussis-ASR-15009-1/

16: Pertussis Parkhill J, et al. (2003). "Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella ... Nieves DJ, Heininger U (2016). "Bordetella pertussis". Bordetella pertussis. Microbiology Spectrum. 4. pp. 311-339. doi:10.1128 ... Bordetella pertussis is a Gram-negative, aerobic, pathogenic, encapsulated coccobacillus of the genus Bordetella, and the ... "Bordetella pertussis epidemiology and evolution in the light of pertussis resurgence". Infection, Genetics and Evolution. 40: ...

https://en.wikipedia.org/wiki/Bordetella_pertussis

Bordetella pertussis pathogenesis: current and future challenges.. Melvin JA1, Scheller EV1, Miller JF2, Cotter PA1. ... a) Pertussis toxin (PT, PDB ID 1PRT), is an AB5-type toxin composed of one catalytic subunit (A subunit) and five membrane- ... b) Bordetella spp. fimbriae are type 1 pili. FimB is similar to chaperone proteins that traffic major fimbrial subunits (Fim2 ... c) Bordetella spp. adenylate cyclase toxin (ACT) is composed of two primary domains, a calmodulin-responsive adenylate cyclase ...

https://www.ncbi.nlm.nih.gov/pubmed/24608338

... , Whooping Cough Test, Pertussis Diagnostics, Pertussis Culture, Pertussis PCR, Bordatella PCR. ... Bordetella Pertussis Test. Bordetella Pertussis Test Aka: Bordetella Pertussis Test, Whooping Cough Test, Pertussis Diagnostics ... Bordetella Pertussis Test Colorimetric Capnometry Delta F508 Diffusing Capacity End-Tidal CO2 End-Tidal O2 Exercise Spirometry ... These images are a random sampling from a Bing search on the term "Bordetella Pertussis Test." Click on the image (or right ...

https://fpnotebook.com/lung/Lab/BrdtlPrtssTst.htm

Bordetella pertussis synonyms, Bordetella pertussis pronunciation, Bordetella pertussis translation, English dictionary ... definition of Bordetella pertussis. n. 1. A part that forms the outer edge of something. 2. A decorative strip around the edge ... Bordetella pertussis - definition of Bordetella pertussis by The Free Dictionary https://www.thefreedictionary.com/Bordetella+ ... Bordetella pertussis infections maybe a common cause of persistent cough in adults, said Dr.. B. Pertussis, Chronic Cough ...

https://www.thefreedictionary.com/Bordetella+pertussis

... ,ARUP Laboratories is a national reference laboratory and a worldwide leader in ... Bordetella pertussis Antibody, IgM. 5. Bordetella pertussis Antibody, IgG. 6. Bordetella pertussis by PCR. 7. Bordetella ... Bordetella pertussis ASR. 2. Bordetella pertussis Antibodies, IgA & IgG. 3. Bordetella pertussis Antibodies, IgG & IgM. 4. ... Bordetella pertussis DFA. 10. Bordetella pertussis Toxin Antibody, IgA. 11. Bordetella pertussis Toxin Antibody, IgG. ...

http://www.bio-medicine.org/medicine-products/Bordetella-pertussis-Antibodies--IgA--IgG---26-IgM-21816-1/

Inhibition of murine allergic airway disease by Bordetella pertussis.. Kim YS1, Kwon KS, Kim DK, Choi IW, Lee HK. ... pertussis (2 × 109 cells) and (b) Varying doses of B. pertussis DNA were administered i.p. 1 day before the first airway ... Ten µg of DNA was mixed with 10 U of CpG methylase for 16 hr or DNase I for 2 hr at 37°. One µg of B. pertussis DNA were ... P , 0·01 versus control **P , 0·05 versus B. pertussis DNA-treated group. (e) PT, LPS, and FHA were inactivated as described in ...

https://www.ncbi.nlm.nih.gov/pubmed/15270734?dopt=Abstract

Pertussis: Not Your Typical URI By Brit Long, MD & Alex Koyfman, MD ...

http://epmonthly.com/keyword/bordetella-pertussis/

Comparative genomics of the classical Bordetella subspecies: the evolution and exchange of virulence-associated diversity ...

http://www.genome.jp/dbget-bin/www_bget?gn:T02296

... tracto respiratorio que se presenta exclusivamente en los seres humanos y es causada por la bacteria gramnegativa Bordetella ... Bordetella Pertussis: Tos Ferina. La tos ferina es una enfermedad del tracto respiratorio que se presenta exclusivamente en los ... Bordetella pertussis. Contienen fimbrias, pilis y cápsula. Son aerobios estrictos. Citotoxina traqueal produce cilio-estasis. ... Transcript of Bordetella Pertussis: Tos Ferina. Son microorganismos cocobacilares gramnegativos.. Patogenia. Factores que ...

https://prezi.com/64px9zhvd6ex/bordetella-pertussis-tos-ferina/

However, a significant prevalence of Prn-deficient (Prn−) B. pertussis was observed in Japan. The Prn− isolate was first ... discovered in 1997, and 33 (27%) Prn− isolates were identified among 121 B. pertussis isolates collected from 1990 to 2009. ... is one of the major virulence factors of Bordetella pertussis, the etiological agent of whooping cough. ... Bordetella pertussis Is the Subject Area "Bordetella pertussis" applicable to this article? Yes. No. ...

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0031985

Helical structure of Bordetella pertussis fimbriae.. A C Steven, M E Bisher, B L Trus, D Thomas, J M Zhang, J L Cowell ... Helical structure of Bordetella pertussis fimbriae. Message Subject (Your Name) has forwarded a page to you from Journal of ... The helical structures of Bordetella pertussis fimbriae of serotypes 2 and 6 were determined by optical diffraction analysis of ...

https://jb.asm.org/content/167/3/968

... industrial applications and more information for Bordetella pertussis. ... Bordetella pertussis is BSL2 organism and should be considered a potential pathogen.. ... Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Alcaligenaceae; Bordetella. Industrial uses or economic ...

http://thelabrat.com/protocols/Bacterialspecies/Bordetellapertussis.shtml

Immunization with agglutinogen 2 protected mice against infection with 1.2.0 or 1.2.3 serotypes of B. pertussis, whereas ... The ability of purified serospecific agglutinogens from Bordetella pertussis to protect mice against intranasal infection has ... Serospecific protection of mice against intranasal infection with Bordetella pertussis Vaccine. 1989 Aug;7(4):321-4. doi: ... The ability of purified serospecific agglutinogens from Bordetella pertussis to protect mice against intranasal infection has ...

https://pubmed.ncbi.nlm.nih.gov/2573215/?dopt=Abstract

Genomic DNA from Bordetella pertussis Strain Tohama 1 TypeStrain=False Application: ... Bordetella pertussis (Bergey et al.) Moreno-Lopez (ATCC® BAA-589D-5™) Strain Designations: Genomic DNA from Bordetella ... Bordetella pertussis (Bergey et al.) Moreno-Lopez ATCC® BAA-589D-5™ dried At least 5 µg in 1X TE buffer. OD260/OD280: 1.6 to ... Quantitative Genomic DNA from Bordetella pertussis (ATCC® BAA-589DQ™) Add to frozen Specification range: ≥ 1 x 105 copies/µL. ...

https://www.atcc.org/en/Products/Cells_and_Microorganisms/Bacteria/Bacterial_Nucleic_Acids/BAA-589D-5.aspx

NAME: Bordetella pertussis. SYNONYM OR CROSS REFERENCE: Originally named Haemophilus pertussis F Footnote 1, Whooping cough ... CHARACTERISTICS: Bordetella pertussis are small, gram-negative, encapsulated, non-motile, coccobacilli with outer pili. They ... Pathogen Safety Data Sheets: Infectious Substances - Bordetella pertussis. PATHOGEN SAFETY DATA SHEET - INFECTIOUS SUBSTANCES. ... Wilson, K. E., Cassiday, P. K., Popovic, T., & Sanden, G. N. (2002). Bordetella pertussis isolates with a heterogeneous ...

https://www.canada.ca/en/public-health/services/laboratory-biosafety-biosecurity/pathogen-safety-data-sheets-risk-assessment/bordetella-pertussis.html

Abcam provides general protocols for Human Anti-Bordetella pertussis IgA ELISA Kit (ab108708). Please download our pdf protocol ...

http://www.abcam.com/human-bordetella-pertussis-iga-elisa-kit-ab108708-protocols.html

Human Bordetella pertussis IgG ELISA Kit is a sensitive immunoassay suitable for the quantification of Bordetella pertussis IgG ... Bordetella pertussis anti-IgG HRP Conjugate. Black cap. 1 x 20ml. Bordetella pertussis IgG Cut-off Control. Green cap. 1 x 3ml ... Human Anti-Bordetella pertussis IgG ELISA Kit. See all Bordetella pertussis IgG kits. ... ELISA - Human Anti-Bordetella pertussis IgG ELISA Kit (ab108709) Protein - Native Human IgG FC fragment protein (ab90285) SDS- ...

https://www.abcam.com/human-bordetella-pertussis-igg-elisa-kit-ab108709.html

Discover a faster, simpler path to publishing in a high-quality journal. PLOS ONE promises fair, rigorous peer review, broad scope, and wide readership - a perfect fit for your research every time. Learn More Submit Now ...

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0113322

Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica. ... Differential regulation of type III secretion and virulence genes in Bordetella pertussis and Bordetella bronchiseptica by a ... The BvgAS Regulon of Bordetella pertussis. Kyung Moon, Richard P. Bonocora, David D. Kim, Qing Chen, Joseph T. Wade, Scott ... The BvgAS Regulon of Bordetella pertussis. Kyung Moon, Richard P. Bonocora, David D. Kim, Qing Chen, Joseph T. Wade, Scott ...

https://mbio.asm.org/content/8/5/e01526-17

Bordetella pertussis. The whooping cough bacteria parasitise only humans. They cause a respiratory tract infection ... Keywords: bacteria, bacterial, bacteriology, bacterium, betaproteobacteria, bordetella pertussis, electron micrograph, infect, ... Bordetella pertussis. The whooping cough bacteria parasitise only humans. They cause a respiratory tract infection ...

http://www.sciencephoto.com/media/10915/view

Viable cell numbers (cfu/ml) of the B. pertussis strains and a rough strain of B. bronchiseptica (CSU-P-1) d … ... pertussis and one strain of B. parapertussis exposed to hyperimmune and pre-colostrum porcine serum was examined. ... The viability of four strains of Bordetella bronchiseptica, two strains of B. ... The viability of four strains of Bordetella bronchiseptica, two strains of B. pertussis and one strain of B. parapertussis ...

https://pubmed.ncbi.nlm.nih.gov/2010907/?dopt=Abstract

If Bordetella pertussis Antibody, IgA by ELISA is 1.2 U/mL or greater, then Bordetella pertussis IgA Immunoblot testing will be ... then Bordetella pertussis IgG Immunoblot testing will be added; If Bordetella pertussis Antibody, IgM by ELISA is 1.2 U/mL or ... BORDETELLA PERTUSSIS ANTIBODIES, IGA, IGG AND IGM BY ELISA WITH REFLEX TO IMMUNOBLOT. Mnemonic ... 86615 x3 Bordetella (IgA, IgG, IgM), if reflexed, add 86615 for each Bordetella Immunoblot ...

https://www.legacyhealth.org/for-health-professionals/refer-a-patient/laboratory-services/samaritan-health-services/bordetella-pertussis-antibodies-iga-igg-and-igm-by-elisa-with-reflex-to-immunoblot

Blue Cross Blue Shield of Massachusetts is a leading provider of quality health insurance for residents of Massachusetts. Our main goal is to make health care cost-effective by offering health insurance solutions for individuals, families, and large and small businesses at affordable rates.. Our Vision - Making Quality Health Care ...

http://www.ahealthyme.com/RelatedItems/RelatedDocuments.pg?d=&TypeId=167&ContentId=bordetella_pertussis_antibody&Category=Tests

Bordetella pertussis, the human pathogen of whooping cough, when grown at 22 degrees C is nonvirulent and unable to bind ... Adhesion of Bordetella pertussis to eukaryotic cells requires a time-dependent export and maturation of filamentous ... Adhesion of Bordetella pertussis to eukaryotic cells requires a time-dependent export and maturation of filamentous ... Adhesion of Bordetella pertussis to eukaryotic cells requires a time-dependent export and maturation of filamentous ...

https://www.pnas.org/content/90/19/9204?ijkey=e66a84158fe4a8fc38a33a301e6690f3c0058e58&keytype2=tf_ipsecsha

Pertussis toxin, the major virulence factor of Bordetella pertussis, is not produced by the closely related species Bordetella ... Bordetella parapertussis and Bordetella bronchiseptica contain transcriptionally silent pertussis toxin genes.. B Aricò, R ... Bordetella parapertussis and Bordetella bronchiseptica contain transcriptionally silent pertussis toxin genes.. B Aricò, R ... Bordetella parapertussis and Bordetella bronchiseptica contain transcriptionally silent pertussis toxin genes.. B Aricò, R ...

https://jb.asm.org/content/169/6/2847?ijkey=0bd68609364dfdcc7b0f6a4cc0d8a07d5b7838f7&keytype2=tf_ipsecsha

Bordetella pertussis. The micrograph shows the bacteriums surface covered in fine hairs called pili or frimbriae. Several ... Keywords: bacteria, bacterial, bacteriology, bacterium, betaproteobacteria, bordetella pertussis, electron micrograph, ... Caption: False colour transmission electron micrograph of the whooping cough bacterium, Bordetella pertussis. The micrograph ...

http://www.sciencephoto.com/media/10907/view

The toxin, known as pertussis toxin (or PTx), inhibits G protein coupling that regulates an adenylate cyclase-mediated conversion of ATP to cyclic AMP. (wikipedia.org)
These findings suggest that B. per- lia contains pertussis toxin (Ptx), pertactin (Prn), and fila- tussis not expressing Prn arose independently multiple mentous hemagglutinin (Fha). (cdc.gov)
How much (µg) pertussis toxin and filamentous hemaggluttinin are coated to the human ELISA IgG plates (per well)? (abcam.com)
Also, is the pertussis toxin the entire complex or just the S1 subunit? (abcam.com)
The pertussis toxin is the entire complex. (abcam.com)
Bordetella parapertussis and Bordetella bronchiseptica contain transcriptionally silent pertussis toxin genes. (asm.org)
Pertussis toxin, the major virulence factor of Bordetella pertussis, is not produced by the closely related species Bordetella parapertussis and Bordetella bronchiseptica. (asm.org)
Nucleotide sequencing of an EcoRI fragment of 5 kilobases comprising the regions homologous to the pertussis toxin genes shows that in this region, B. parapertussis and B. bronchiseptica are 98.5% and 96% homologous, respectively, to B. pertussis. (asm.org)
iii) the presence of pertussis toxin (PT)-, filamentous haemagglutinin (FHA)- or fimbriae (Fim)-deficient isolates. (eurosurveillance.org)
and shown to be a protective antigen in the mouse intracerebral protection assay when nonprotective levels of pertussis toxin were present ( 11 ). (asm.org)
Adenylate cyclase-hemolysin toxin (CyaA) produced from the human respiratory tract pathogen Bordetella pertussis requires fatty-acyl modification by CyaC-acyltransferase to become an active toxin. (sigmaaldrich.com)
Using data on 195 participants (≥7 years old) from an epidemiological study, we assessed the sensitivity, specificity, and performance (Youden index) for pertussis diagnosis of the pertussis toxin enzyme-linked immunosorbent assay (using single and paired serology) and of real-time PCR assays (using the IS 481 and ptxA -Pr targets). (asm.org)
Immunization with the Recombinant Cholera Toxin B Fused to Fimbria 2 Protein Protects against Bordetella pertussis Infection. (biomedsearch.com)
This study examined the immunogenic properties of the fusion protein fimbria 2 of Bordetella pertussis (Fim2)-cholera toxin B subunit (CTB) in the intranasal murine model of infection. (biomedsearch.com)
To this end B. pertussis Fim2 coding sequence was cloned downstream of the cholera toxin B subunit coding sequence. (biomedsearch.com)
Pertussis toxin CPTD enhanced aggregation of rabbit peritoneal and peripheral neutrophils and human peripheral neutrophils. (gla.ac.uk)
Studies on immunized children have suggested that high levels of circulating Abs against the B. pertussis virulence factors, pertussis toxin (PT), and pertactin may be important for protection ( 4 , 5 ). (jimmunol.org)
A characterisation has been made for the pertussis toxin (pt), the filamentery haemagglutinine (fha) and different lipopolysaccharides (lps). (rapidtest.com)
The use of purified materials confirmed that the presence of this pertussis toxin (PT) was responsible for the later peak in stimulation, whereas lipopolysaccharide (LPS) in combination with PT and also the filamentous haemagglutinin (FHA) could mimic the early peak of stimulation. (eurekamag.com)
The former document is a consensus document on laboratory guidance for the determination of serum Immunoglobulin G (IgG) anti-pertussis toxin antibodies to pertussis by enzyme-linked immunosorbent assay (ELISA) serology for European laboratories. (europa.eu)
Anti-pertussis toxin (PT), anti-filamentous hemagglutinin (FHA), and anti-pertactin (PRN) IgG titers were measured before primary vaccination, and before and after booster vaccination. (biomedcentral.com)
The quantitative determination of specific Immunoglobulin G antibodies to Bordetella pertussis toxin was done using a commercially available validated ELISA method. (biomedcentral.com)
Construction of Bordetella pertussis strains with enhanced production of genetically-inactivated Pertussis Toxin and Pertactin by unmarked allelic exchange. (openrepository.com)
Journal Article] Polymorphisms Influencing Expression of Dermonecrotic Toxin in Bordetella bronchiseptica. (nii.ac.jp)
PCR was done using oligonucleotide primers PTp1 and PTp2 which amplify a 191-base pair DNA fragment of pertussis toxin operon. (ovid.com)
Recombinant, genetically-detoxified adenylate cyclase toxin (CyaA) constructs from Bordetella pertussis have been developed as potential antigen delivery systems and as promising antigen candidates for inclusion in acellular pertussis vaccines. (synbiosis.com)
This Pertussis toxin, CAS 70323-44-3, catalyzes ADP-ribosylation of guanine nucleotide-binding regulatory proteins Gi, Go, and Gt. (emdmillipore.com)
Ross, P.J., Lavelle, E.C., Mills, K.H.G. and A.P. Boyd `Adenylate cyclase toxin from Bordetella pertussis synergises with lipopolysaccharide to promote innate IL-10 production and enhance the induction of Th2 and regulatory T cells? (tcd.ie)
Adenylate cyclase toxin (CyaA) from Bordetella pertussis can subvert host immune responses allowing bacterial colonization. (tcd.ie)
The adenylate cyclase toxin, along with several other B. pertussis virulence factors, undergo a phase shift between states of expression and repression. (openrepository.com)
They may include pertussis toxin (PT), filamentous hemagglutin (FHA), Prn, or Fim2 and Fim3. (biomedcentral.com)
This rose to 92 % (105/114) if only samples positive for both of the two targets used for the B. pertussis PCR (insertion element IS 481 and pertussis toxin promoter ptxP ) were analysed. (microbiologyresearch.org)
Sequence-based typing of the pertactin, pertussis toxin S1 subunit and pertussis promoter regions ( prn , ptxA and ptxP ) was attempted on 89 of the DNA extracts that had generated a full MLVA profile. (microbiologyresearch.org)
Pulsed-field gel electrophoresis, pertactin, pertussis toxin S1 subunit polymorphisms, and surfaceome analysis of vaccine and clinical Bordetella pertussis strains. (microbiologyresearch.org)
First report and detailed characterization of B. pertussis isolates not expressing pertussis toxin or pertactin. (microbiologyresearch.org)
Results for Bordetella culture and/or direct fluorescent antibody testing and a second LightCycler PCR assay (target, pertussis toxin gene) were compared to results of the LC-PCR-IS assay for 111 nasopharyngeal swabs submitted for pertussis testing. (elsevier.com)

Pertussis, also known as whooping cough , is a highly contagious respiratory disease. (cdc.gov)
After cough fits, someone with pertussis often needs to take deep breaths, which result in a "whooping" sound. (cdc.gov)
Bordetella pertussis is a Gram-negative, aerobic, pathogenic, encapsulated coccobacillus of the genus Bordetella, and the causative agent of pertussis or whooping cough. (wikipedia.org)
The paroxysmal cough precedes a crowing inspiratory sound characteristic of pertussis. (wikipedia.org)
Pertussis, also known as whooping cough, has recently re-emerged as a major public health threat despite high levels of vaccination against the aetiological agent Bordetella pertussis. (nih.gov)
Bordetella pertussis causes whooping cough, a severe respiratory tract infection in infants and children, and also infects adults. (nih.gov)
Bordetella pertussis was isolated from a patient with whooping cough. (thelabrat.com)
Originally named Haemophilus pertussis F Footnote 1 , Whooping cough Footnote 2 . (canada.ca)
B. pertussis is a respiratory pathogen that causes pertussis, also known as whooping cough, a localized infection of the ciliated epithelium of the bronchial tree Footnote 3 , Footnote 7 . (canada.ca)
The second stage is the paroxysmal stage, lasting for about 1-6 weeks, and is characterized by various pathognomonic symptoms of pertussis such as episodes of paroxysmal cough with a characteristic whooping sound. (canada.ca)
Atypical pertussis, a less severe respiratory illness which include prolonged, nonparoxysmal cough occurs mainly in older immunized children and adults Footnote 3 , Footnote 7 . (canada.ca)
Pertussis infection in infants may cause severe cough, choking, poor feeding, and apnea without paroxysmal cough Footnote 3 , Footnote 7 , Footnote 10 . (canada.ca)
False colour transmission electron micrograph (TEM) of a thin section of the whooping cough bacteria, Bordetella pertussis. (sciencephoto.com)
Bordetella pertussis, the human pathogen of whooping cough, when grown at 22 degrees C is nonvirulent and unable to bind eukaryotic cells. (pnas.org)
Bordetella pertussis, the main causative agent of whooping cough, is a reemerging pathogen, and recent vaccine-resistant strain outbreaks and emergence of macrolides-resistant strains in China raised new concerns for control of the disease. (medworm.com)
Infectious diseases such as smallpox, measles, diphtheria and pertussis (whooping cough) were once prevalent and killed indiscriminately. (medworm.com)
Pertussis or whooping cough is a highly contagious vaccine-preventable respiratory disease that resurged during the last decades in many countries. (medworm.com)
Despite high vaccination coverage, Bordetella pertussis the causative agent of whooping cough is still a health concern worldwide. (medworm.com)
For this study, we employed the same quantitative, immunofluorescent adherence assay to test the possibility that sera of patients recovering from naturally acquired whooping cough or immunized with pertussis vaccine may contain activity capable of interfering with this specific adherence. (asm.org)
Bordetella pertussis is the causative agent of the disease whooping cough. (asm.org)
Despite high pertussis vaccine coverage, reported cases of whooping cough (pertussis) have increased over the last decade in the United States and other developed countries. (pubmedcentralcanada.ca)
IMPORTANCE Whooping cough, primarily caused by Bordetella pertussis , has resurged in the United States even though the coverage with pertussis-containing vaccines remains high. (pubmedcentralcanada.ca)
Bordetella pertussis is the causative agent of whooping cough (pertussis), a respiratory disease with the highest morbidity and mortality in young infants. (pubmedcentralcanada.ca)
CyaA is crucial for colonization by Bordetella pertussis, the etiologic agent of whooping cough. (rcsb.org)
Immunization with whole cell pertussis vaccines (Pw) 3 is effective at preventing whooping cough in children. (jimmunol.org)
Whooping cough is a disease of the respiratory tracts which is caused by Bordetella pertussis bacteria. (rapidtest.com)
Pertussis or whooping cough is currently the most prevalent vaccine-preventable childhood disease despite >85% global vaccination coverage. (openrepository.com)
Bordetella pertussis is the etiological agent of whooping cough, a bacterial infection of especially children, which may be fatal without treatment. (biomedcentral.com)
In 2008 about 16 million cases of whooping cough-also designated as pertussis-resulting in almost 200,000 pediatric deaths were estimated by the World Health Organization, while for 2015 more than 60,000 deaths of infants were assumed [ 1 , 2 , 3 ]. (biomedcentral.com)
Bordetella pertussis , the gram-negative bacterium that causes whooping cough, still infects up to 40 million individuals each year and is responsible for around 300,000 annual deaths ( 27 ), despite a high worldwide vaccine coverage. (asm.org)
Since 2011, increases in the number of cases of pertussis, commonly known as whooping cough, have been repeatedly reported across the world, even in those with sustained high vaccination coverage. (europa.eu)
The guidance and protocol is intended for real-time PCR on DNA extracted from clinical specimens obtained from patients with suspected whooping cough (i.e. infection with Bordetella pertussis or B. parapertussis ). (europa.eu)
Both documents have been produced by the members of the European Bordetella expert group 'EUpert-Labnet' as part of "Coordination of activities for laboratory surveillance of whooping cough in Member States and EEA countries" project. (europa.eu)
We tried to identify a causative factor of Bordetella pertussis that causes paroxysmal coughing in the human infection, whooping cough. (nii.ac.jp)
For this purpose, we established an animal experimental model that replicates coughing similar to that observed in whooping cough by using B. bronchiseptica, which is closely related to B. pertussis. (nii.ac.jp)
Bordetella pertussis is the causative agent of whooping cough, a respiratory disease still considered as a major public health threat and for which recent re-emergence has been observed. (altmetric.com)
We have previously identified three novel loci that influence the severity of whooping cough by using recombinant congenic strains of mice: Bordetella pertussis susceptibility loci 1, 2, and 3 (Bps1, -2, and -3). (synbiosis.com)
The genetic composition of the bacterium causing whooping cough, Bordetella pertussis , has been investigated using microarray studies in order to examine potential genetic contributors to the disease re-emergence in the past decade. (biomedcentral.com)
B. pertussis are the bacteria that cause pertussis, or whooping cough. (baylorhealth.com)
Clinicians should consider pertussis in the differential diagnosis of persistent cough illness in people of all ages - even those previously immunised. (cancerscreening.gov.au)
Whooping cough, caused by the bacterium Bordetella pertussis , is a highly contagious respiratory disease that can cause serious illness or death among infants and young children. (cancerscreening.gov.au)
In April 1999, within a remote community in the Gascoyne region of WA, an elderly male with a history of chronic cough was diagnosed as having B. pertussis by IgA serology. (cancerscreening.gov.au)
On 24 November, after a woman and her daughter presented with a persistent cough, a local general practitioner concerned about pertussis contacted the Gascoyne Public Health Unit (PHU) and organised nasopharyngeal aspirates. (cancerscreening.gov.au)
Bordetella pertussis is the causative agent of human whooping cough (pertussis) and is particularly severe in infants. (biomedcentral.com)
Bordetella pertussis is the causative agent of whooping cough, a highly contagious disease of the respiratory tract in humans. (biomedcentral.com)
Mycoplasma pneumoniae and Chlamydophila pneumoniae (atypical pathogens) and Bordetella pertussis ('pertussis' or 'whooping cough') respiratory tract infections are often diagnosed clinically while waiting for laboratory results to come back, which take days. (isrctn.com)
Whooping cough (pertussis) is a consequence of upper respiratory tract colonisation by B. pertussis and is characterised by symptoms such as fever and coughing, which persist for more than a week. (fiocruz.br)
Pertussis is a highly infectious vaccine-preventable cough illness that continues to be a significant source of morbidity and mortality around the world. (asmscience.org)
Pertussis (whooping cough) is a severe respiratory disease in children caused by a bacterial infection with Bordetella pertussis - the mortality rate is 0.5% in infants under six months. (roche.com)
The disease pertussis, more commonly known as whooping cough, is caused by the gram negative bacterium Bordetella pertussis. (openrepository.com)
Bordetella pertussis is a causative agent of pertussis or whooping cough in humans. (biomedcentral.com)
Pertussis or whooping cough is an infectious respiratory disease caused by the bacterium Bordetella pertussis . (biomedcentral.com)

30% (96/320) of B. pertussis isolates did not express the (ACV) was developed in the 1980s. (cdc.gov)
Although ACV formulations differ in the number of sion of Prn in 16 (17%) isolates could not be determined component pertussis antigens, the vaccine used in Austra- at the sequence level. (cdc.gov)
Rapid Increase in Pertactin-deficient B. pertussis of age) and increased use of more sensitive diagnostic tests, A total of 126 additional isolates collected from Westmead such PCR ( 4 ). (cdc.gov)
In a dot enzyme immunoassay, HBp2 reacted with all B. pertussis strains and clinical isolates tested except for four atypical variant strains of the LOS B phenotype. (nih.gov)
High prevalence of pertactin (PRN)-deficient Bordetella pertussis isolates has been found in these countries. (eurosurveillance.org)
B. pertussis clinical isolates were obtained from different European countries during four periods (EUpert I-IV studies): 1998 to 2001 (n = 102), 2004 to 2005 (n = 154), 2007 to 2009 (n = 140) and 2012 to 2015 (n = 265). (eurosurveillance.org)
Although Bordetella pertussis is well known for its limited gene sequence variation, recent advances in long-read sequencing technology have begun to reveal genomic structural heterogeneity among otherwise indistinguishable isolates, even within geographically or temporally defined epidemics. (pubmedcentralcanada.ca)
We have compared rearrangements among complete genome assemblies from 257 B. pertussis isolates to examine the potential evolution of the chromosomal structure in a pathogen with minimal gene nucleotide sequence diversity. (pubmedcentralcanada.ca)
It is also required for the determination of the appropriate vaccine strain and the antimicrobial susceptibility of isolates in order to control the spread of pertussis ( 12 , 13 ). (pubmedcentralcanada.ca)
Bordetella pertussis isolates in Finland: serotype and fimbrial expression. (openrepository.com)
Bordetella pertussis isolates lacking pertactin, a key antigen component of the acellular pertussis vaccine, have been observed, suggesting that B. pertussis is losing pertactin in response to vaccine immunity. (asm.org)
Screening of 1,300 isolates from outbreak and surveillance studies (historical isolates collected from 1935 up to 2009, isolates from the 2010 California pertussis outbreak, U.S. isolates from routine surveillance between 2010-2012, and isolates from the 2012 Washington pertussis outbreak) by conventional PCR and later by Western blotting and prn sequencing analyses ultimately identified 306 pertactin-deficient isolates. (asm.org)
All Bordetella pertussis isolates were macrolide-resistant, harbored prn1/ptxP1/fim3-1 as previously reported and belonged to multilocus variable tandem repeat analysis type MLVA 195. (ovid.com)
Regions of difference (RDs) have been previously identified as clusters of genes flanked by insertion sequences which are variably present in different sets of isolates, and have also been shown to be potential markers of B. pertussis evolution. (biomedcentral.com)
A B. pertussis specific mPCR/RLB using 43 genes representing 30 RDs, was developed and used to characterise a set of 42 B. pertussis isolates. (biomedcentral.com)
We previously used single nucleotide polymorphisms (SNPs) to characterise an international collection of over 300 B. pertussis isolates. (biomedcentral.com)
These isolates were differentiated into 42 unique SNP profiles (SPs) and six distinct clusters (clusters I to VI) [ 14 ], with recently emerging isolates in cluster I. In this study, we used representative isolates of each of the 42 SPs to develop an inexpensive specific mPCR/RLB to further determine the genome variation in B. pertussis . (biomedcentral.com)
In an attempt to gain insight into the genomic make-up of B. pertussis over the last 60 years, we used an oligonucleotide DNA microarray to compare the genomic contents of a collection of 171 strains of B. pertussis isolates from different countries. (biomedcentral.com)
An overview of genomic contents of a large collection of isolates from different countries allowed us to derive a core genome and a phylogenetic structure of B. pertussis . (biomedcentral.com)
We examined the regulation of fimbrial expression in B. pertussis clinical isolates. (pasteur.fr)
Molecular typing of Bordetella pertussis is routinely performed on bacterial isolates, but not on DNA extracted from nasopharyngeal aspirates or pernasal swabs submitted for diagnostic real-time PCR (qPCR). (microbiologyresearch.org)
Comparison of molecular typing data from the 89 extracts with those from 111 contemporary bacterial isolates showed that the two sources yielded the same picture of the B. pertussis population [dominated by the MLVA-27 prn (2) ptxA (1) ptxP (3) clonal type]. (microbiologyresearch.org)
Subtractive hybridization was carried out to identify differences between the sequenced genome of Bordetella pertussis Tohama I and those of two recently collected isolates. (pasteur.fr)

2020 Sep 28;137:155313 Authors: da Silva Antunes R, Quiambao LG, Soldevila F, Sutherland A, Peters B, Sette A Abstract Bordetella Pertussis (BP) vaccine-induced immunity is waning worldwide despite excellent vaccine coverage. (medworm.com)

Whole-cell pertussis vaccines have been shown to selectively induce T helper 1 (Th1)-type responses in human and animals. (nih.gov)
Acellular vaccines against Bordetella pertussis were implementation of immunization with a whole-cell vaccine introduced in Australia in 1997. (cdc.gov)
Whole cell pertussis vaccines (wP) are effective, but reactogenic, and have been replaced in most developed countries by acellular pertussis vaccines (aP). (nih.gov)
Pertussis outbreaks have occurred in several industrialised countries using acellular pertussis vaccines (ACVs) since the 1990s. (eurosurveillance.org)
A resurgence of pertussis cases has been reported, particularly in countries using acellular vaccines with waning immunity and pathogen adaptation thought to be responsible. (medworm.com)
The mouse body weight gain test (MWGT), the leukocytosis-promoting test (LPT), and the histamine sensitization test (HIST) are done for the testing of the specific toxicity of pertussis vaccines. (omicsonline.org)
The introduction of vaccines against pertussis during the 1940s dramatically reduced the disease incidence in the United States. (pubmedcentralcanada.ca)
However, despite high or increasing coverage with pertussis-containing vaccines, the number of reported pertussis cases in the United States and many other developed countries has increased over the last decade, with notable recent epidemics ( 1 , - 3 ). (pubmedcentralcanada.ca)
Whole cell pertussis vaccines (Pw) induce Th1 responses and protect against Bordetella pertussis infection, whereas pertussis acellular vaccines (Pa) induce Ab and Th2-biased responses and also protect against severe disease. (jimmunol.org)
However, Pw have been associated with a number of local and systemic reactions, and although still used in developing countries, have been replaced in developed countries by acellular pertussis vaccines (Pa), prepared with highly purified Ags from Bordetella pertussis administered with alum as the adjuvant ( 1 , 2 ). (jimmunol.org)
Unlike other pertussis vaccines, BPZE1 has been shown to provide strong protection against infection by the causative agent of pertussis, Bordetella pertussis, in non-human primates. (openrepository.com)
Increasing FIM2/3 antigen-content improves efficacy of Bordetella pertussis vaccines in mice in vivo without altering vaccine-induced human reactogenicity biomarkers in vitro. (openrepository.com)
Bordetella pertussis fimbriae (Fim): relevance for vaccines. (openrepository.com)
In frame of studies to investigate putative effects of vaccination on host-pathogen interaction and clonal distribution of strains, in addition to Corynebacterium diphtheriae and Clostridium tetani toxoid vaccines, also whole-cell and acellular pertussis vaccines were analyzed by mass spectrometry. (biomedcentral.com)
LC-MS/MS spectra were generated and analyzed using B. pertussis genome data and proteins present in whole-cell and acellular pertussis vaccines were identified. (biomedcentral.com)
Whole-cell vaccines are suspensions of killed B. pertussis cells, while the acellular pertussis vaccines, which were developed to prevent unwanted local reactions of the whole-cell vaccine, contain only a limited number of purified components (for overview of different vaccine formulations see [ 3 ]. (biomedcentral.com)
The data represent proteomic analyses of commercially vaccines, an acellular and a whole cellular pertussis vaccine. (biomedcentral.com)
Bordetella pertussis vaccines are typically administered in combination with diphtheria and tetanus toxoid vaccines as DTP3 vaccines. (biomedcentral.com)
However, the immune mechanism of protection against B. pertussis and, consequently, the optimal way to administer pertussis vaccines, especially in young children, are still insufficiently understood. (asm.org)
After booster vaccination, no significant difference was observed between the two vaccines in antibody titers against pertussis antigens ( P = 0.53 for anti-PT IgG, P = 0.91 for anti-FHA IgG, P = 0.39 for anti-PRN IgG). (biomedcentral.com)
When mapped against the previously identified evolutionary relationships of the strains, the losses of two RDs - BP0910A - BP00930 and BP1948-BP1962 - were found to be associated with significant events in B. pertussis history: the loss of BP0910A - BP00930 coincided with introduction of whole cell vaccines in the 1950s while that of BP1948-BP1962 occurred after the introduction of acellular vaccines. (biomedcentral.com)
2 The school entry dose was introduced in August 1994 and acellular pertussis vaccines replaced whole cell ones for booster doses in Western Australia (WA) in February 1997 (Source: Perth Immunisation Clinic). (cancerscreening.gov.au)
Although immunisation with a cellular vaccine was effective in the late XX century, this type of vaccine has been replaced by acellular pertussis vaccines in several countries due to its side effects. (fiocruz.br)
Pertactin (Prn), fimbriae 2 (Fim2) and fimbriae 3 (Fim3) of B. pertussis are important virulence factors and immunogens which have been included in some acellular pertussis vaccines. (biomedcentral.com)
Our results indicated that the recombinant proteins still retain their immunological properties and highlighted the potential of the recombinant proteins for the future development of the B. pertussis vaccines. (biomedcentral.com)

Southern blot analysis indicates that strains of Bordetella bronchiseptica and Bordetella parapertussis have DNA homologous to vag-8 . (asm.org)
Strains of Bordetella were grown on Bordet-Gengou (BG) medium supplemented with 12% sheep blood or in cyclodextrin solid medium (CSM) ( 1 ). (asm.org)
The work was done with crude fractions and purified components of the bacterium and also whole-cells of mutant strains and of antigenically-modulated variants of B. pertussis. (gla.ac.uk)
The concentration of cefdinir was set by the MIC data from 50 clinical strains of M. catarrhalis and 40 clinical strains of B. pertussis . (pubmedcentralcanada.ca)
Also a change in the fimbrial serotype of B. pertussis strains was observed from serotype Fim2 predominantly found in unvaccinated populations to serotype Fim3 and serotype Fim2,3 in vaccinated populations [ 9 ]. (biomedcentral.com)
We therefore screened 11 inbred strains of mice for susceptibility to a pertussis infection after intranasal infection. (synbiosis.com)
By comparing the presence or absence of RDs, we aimed to determine the genomic variability of a diverse collection of B. pertussis strains and how they have changed over time. (biomedcentral.com)
The loss of BP1948-BP1962 also coincided with expansion of the most recent B. pertussis strains. (biomedcentral.com)
The mPCR/RLB assay offers an inexpensive and fast method of determining the gene content of B. pertussis strains and also confirms that gene losses are an ongoing feature of B. pertussis evolution. (biomedcentral.com)
Changes in the genomic content of circulating Bordetella pertussis strains isolated from the Netherlands, Sweden, Japan and Australia: adaptive evolution or drift? (biomedcentral.com)
The CGH microarray analysis estimated the core genome of B. pertussis , to consist of 3,281 CDSs that are conserved among all B. pertussis strains, and represent 84.8% of all CDSs found in the 171 B. pertussis strains. (biomedcentral.com)
CGH data also revealed that the genome size of B. pertussis strains is decreasing progressively over the past 60 years. (biomedcentral.com)
B. pertussis strains with the same gene content were found in several different countries. (biomedcentral.com)
However, geographic specificity of the B. pertussis strains was not observed. (biomedcentral.com)
The emergence of strains harbouring allelic variants of the antigens used in vaccine development is one of the possible causes of pertussis resurgence. (fiocruz.br)
2010). A recent study based on whole-genome analyses evaluated B. pertussis strains recovered worldwide, though no Brazilian strain was included. (fiocruz.br)
2014). Although there has been a lack of characterisation of strains circulating in Brazil, pertussis has also reemerged in this country. (fiocruz.br)

B. pertussis, B. parapertussis and B. bronchiseptica form a closely related phylogenetical group. (wikipedia.org)
Antiserum raised to a fusion of maltose binding protein to an N-terminal 60-kDa fragment of Vag8 recognizes the native 95-kDa protein in immunoblots of B. pertussis and B. bronchiseptica but not B. parapertussis . (asm.org)
The efficacy of a whole cell pertussis vaccine and fimbriae against Bordetella pertussis and Bordetella parapertussis infections in a respiratory mouse model. (rivm.nl)
Bordetella pertussis or B. parapertussis). (europa.eu)
28 grew B. pertussis and 9 grew B. parapertussis. (ovid.com)
The genus Bordetella consists of Gram-negative β -proteobacteria, including the three human pathogens Bordetella pertussis , Bordetella parapertussis and Bordetella bronchiseptica , which are considered the classical Bordetella species (van der Zee et al. (fiocruz.br)
Common targets for detection of B. pertussis and B. parapertussis are the insertion sequences (IS). (roche.com)
A rapid real-time multiplex PCR assay for detecting and differentiating Bordetella pertussis and Bordetella parapertussis in nasopharyngeal swabs was developed. (elsevier.com)
This assay (LC-PCR-IS) targets the insertion sequences IS481 and IS1001 of B. pertussis and B. parapertussis, respectively, and is performed using the LightCycler (Roche Molecular Biochemicals, Indianapolis, Ind. (elsevier.com)
Of the specimens, 12 were positive (9 B. pertussis and 3 B. parapertussis) and 68 specimens were negative by all methods. (elsevier.com)
All of these characteristics represent a significant improvement in the detection of B. pertussis and B. parapertussis in nasopharyngeal specimens. (elsevier.com)

The heterohybridoma cell line HBp2 secreting human monoclonal antibody (hMAb) directed against Bordetella pertussis was generated by fusing SP2/HPT heteromyeloma cells with human spleen lymphocytes, after in vitro stimulation for 6 days. (nih.gov)
Together these studies suggested that HBp2 is reactive with carbohydrate epitopes present on the LOS A. Binding assays with live bacteria demonstrated that hMAb HBp2 reacted with cell surface exposed epitopes on B. pertussis but the antibody did not bind significantly to the surface on intact B. bronchiseptica cells. (nih.gov)
Characterization of antibody inhibiting adherence of Bordetella pertussis to human respiratory epithelial cells. (asm.org)
Suppression of the antibody response occurred when pertussis cells were injected at the same time as an optimal immunizing dose of SIII. (jimmunol.org)
In contrast, the antibody response to high doses of SIII was enhanced by B. pertussis. (jimmunol.org)
However, when B. pertussis was administered with either optimal or high doses of SIII, 7S as well as 19S antibody against SIII was produced. (jimmunol.org)
Only one adult human serum, from a physician constantly working with B. pertussis, inhibited the mitogenic response to LPF and this serum was shown to contain precipitating antibody against LPF. (eurekamag.com)
The Diagnostic Automation Bordetella pertussis IgA Antibody ELISA Test Kit has been designed for the detection and the quantitative determination of specific IgA antibodies against Bordetella pertussis in serum and plasma. (rapidtest.com)
The Diagnostic Automation Bordetella pertussis IgA antibody test kit is based on the principle of the enzyme immunoassay (EIA). (rapidtest.com)
Antibody responses to Bordetella pertussis Fim2 or Fim3 following immunization with a whole-cell, two-component, or five-component acellular pertussis vaccine and following pertussis disease in children in Sweden in 1997 and 2007. (openrepository.com)
Antibody responses to individual Bordetella pertussis fimbrial antigen Fim2 or Fim3 following immunization with the five-component acellular pertussis vaccine or to pertussis disease. (openrepository.com)
The humoral side of the adaptive immunity was initially considered the main effector of protection against B. pertussis , but there appears to be no direct correlation between serum antibody titers and protection ( 6 , 7 ). (asm.org)
Using a murine respiratory challenge model we have previously demonstrated a role for Th1 cells in natural immunity against Bordetella pertussis , but could not rule out a role for antibody. (rupress.org)
You need info about Human Bordetella pertussis IgM antibody ELISA Kit or any other Gentaur produtct? (gentaurshop.com)

The complete B. pertussis genome of 4,086,186 base pairs was published in 2003. (wikipedia.org)
Bordetella pertussis strain Tohama I, complete genome. (atcc.org)
BX470248 Bordetella pertussis strain Tohama I, complete genome. (atcc.org)
These data illustrate that structural genome evolution in B. pertussis is not limited to reduction but also includes rearrangement. (pubmedcentralcanada.ca)
The chromosome of B. pertussis includes a large number of repetitive mobile genetic elements that obstruct genome analysis. (pubmedcentralcanada.ca)
Constant reshuffling of Bordetella pertussis genome organization was observed during evolution. (altmetric.com)
A repetitive insertion sequence, IS481, has been found to be present in up to 100 copies within the genome of B. pertussis. (loinc.org)
Here, the genome of a clinical Bordetella pertussis strain (Bz181) that was recently isolated in Brazil is reported. (fiocruz.br)
Here, we report the first B. pertussis genome sequence of a clinical strain isolated in the northeastern region of Brazil (Bz181), in 2013. (fiocruz.br)

Bordetella pertussis is the gram-negative coccobacil- doses of ACV at 2, 4, and 6 months of age, and a booster lus that causes the respiratory disease pertussis, also vaccination at 4 years of age. (cdc.gov)
Laboratory confirmation of pertussis is integral to surveillance, especially to monitor the effectiveness of vaccination strategies and inform any changes to national policies. (europa.eu)
The findings suggest that natural infection or vaccination are associated with the acquisition of serum activity inhibiting the adherence of B. pertussis to ciliated cells. (asm.org)
However, vaccination with BPZE1f3 provided significantly stronger protection against Fim3-only producing B. pertussis than vaccination with BPZE1, indicating that immune responses to fimbriae contribute to serotype-specific protection against B. pertussis infection. (openrepository.com)
These numbers of worldwide cases illustrate that pertussis is a continuing threat to children's health and vaccination is important to prevent this infection. (biomedcentral.com)
Tetanus-reduced dose diphtheria-acellular pertussis (Tdap) vaccination during adolescence was introduced in response to the resurgence of pertussis in various countries. (biomedcentral.com)
In Sri Lanka pertussis continues to circulate in the community and cases among adolescents and adults have been reported despite 95% coverage of the four dose pertussis vaccination during early childhood. (biomedcentral.com)
Therefore information on immunity to pertussis in the adolescent group is needed prior to considering vaccination schedule changes. (biomedcentral.com)
90%) in the incidence and mortality due to pertussis was observed following large-scale vaccination during the 1950s and 1960s [ 7 ]. (biomedcentral.com)
Prevention of pertussis outbreaks depends not only on high vaccination coverage among young children but also early diagnosis and management of cases and their contacts. (cancerscreening.gov.au)
In most industrialized countries, generalized vaccination with a whole cell pertussis vaccine (WCV) began between 1950 and 1960. (biomedcentral.com)
Immunization schedules and the type of vaccine used began to differ in each country, leading to disparities in pertussis vaccination histories. (biomedcentral.com)
In the Netherlands, pertussis vaccination, using a WCV produced by the Netherlands Vaccine Institute, was introduced in 1953. (biomedcentral.com)
This study indicated the existence of at least two major B. pertussis lineages related to the pre and post-vaccination periods (Harvill et al. (fiocruz.br)

It is caused by the bacterium Bordetella pertussis . (cdc.gov)
Bordetella pertussis, a human pathogenic bacterium, produces either one or two types of serologically distinct fimbriae, Fim2 and Fim3, as virulence factors. (pasteur.fr)

La tos ferina es una enfermedad del tracto respiratorio que se presenta exclusivamente en los seres humanos y es causada por la bacteria gramnegativa Bordetella pertusis. (prezi.com)
Here, we developed a scalable, rapid, high-throughput luminescence-based Bordetella growth inhibition assay (BGIA) to quantify surviving bacteria after treatment with anti-B. pertussis compounds. (medworm.com)
When fixed, paraffin-embedded sections of human cancers were re-hydrated, incubated with B. pertussis , and then stained with LPHA for β1,6-branched N-glycans, bacteria were seen attached specifically to the sugar-rich regions of the tumors. (aacrjournals.org)
Regarding safety, i.v.-injected B. pertussis showed no toxicity toward the mice after repeated injections of the highest dose of bacteria tested (5 × 10 8 cfu/mouse). (aacrjournals.org)
The induction of protective Th1 responses by previous infection with B. pertussis or by immunization with Pw has been associated with IL-12 production by macrophages or dendritic cells (DC), and this has been linked with LPS and active PT present in the live bacteria and residual endotoxin in Pw preparations ( 14 ). (jimmunol.org)
The cellular immune responses of Balb/c mice and Wistar rats immunized in hind footpads with intact killed Bordetella pertussis were found to differ from those of similar animals immunized with other bacteria including Bordetella bronchiseptica, Salmonella typhimurium and Escherichia coli. (eurekamag.com)
Whole cell pertussis vaccine (wP) which is composed of killed entire bacteria induces a broad immune response against many bacterial antigens (including PT, FHA). (biomedcentral.com)
The B. pertussis Bz181 strain is deposited in the Collection of Bacteria of the Environment and Health at the Oswaldo Cruz Foundation (Fiocruz), Brazil. (fiocruz.br)
Recipients of CD4+ T cells cleared the bacteria from the lungs within 20 days of challenge, at which time B. pertussis-specific antibodies in the serum were undetectable. (tcd.ie)
Typically current APVs are comprised of antigens directly purified from cultured B. pertussis bacteria. (biomedcentral.com)

Resurgence of pertussis in Europe. (microbiologyresearch.org)

Like B. bronchiseptica, B. pertussis is motile and expresses a flagellum-like structure. (wikipedia.org)
When examined for bactericidal activity in the presence of complement, hMAb HBp2 showed high lytic capability against B. pertussis while no killing was obtained against B. bronchiseptica. (nih.gov)
Journal Article] The bvg-repressed gene brtA, encoding biofilm-associated surface adhesin, is expressed during host infection by Bordetella bronchiseptica. (nii.ac.jp)
Journal Article] Detection of genes expressed in Bordetella bronchiseptica colonizing rat trachea by in vivo expressed-tag immunoprecipitation method. (nii.ac.jp)

This antigen merits further investigation as a potentially important component in human immunity to B. pertussis infection. (nih.gov)
Our findings also demonstrate that activation of innate immune cells through TLR4 helps to direct the induction of Th1 and Th-17 cells, which mediate protective cellular immunity to B. pertussis . (jimmunol.org)
However, despite a reduction in IFN-γ production, the rate of bacterial clearance is not significantly lower following B. pertussis challenge of naive or Pw-immunized IL-12p35-defective mice (M. T. Brady and K. H. G. Mills, unpublished observation), suggesting a redundant role for IL-12 in protective adaptive immunity to B. pertussis . (jimmunol.org)
Although the exact causative factors are yet to be defined for the persistent circulation of pertussis, it may be due to the following reasons: waning of immunity induced by immunization, better awareness of the disease, improvement of the diagnostic methods and adaptation of Bordetella pertussis to survive in vaccinated populations. (biomedcentral.com)
Even in highly immunised populations periodic pertussis outbreaks are inevitable reflecting a vaccine efficacy of about 80 per cent and waning immunity with increasing age. (cancerscreening.gov.au)
Reasons for the resurgence of reported pertussis may include molecular changes in the organism and increased awareness and diagnostic capabilities, as well as lessened vaccine efficacy and waning immunity. (asmscience.org)
Mills, K.H.G., Barnard, A.L., Watkins, J. and Redhead, K. `Cell mediated immunity to Bordetella pertussis: role of Th1 cells in bacterial clearance in a murine respiratory infection model? (tcd.ie)
A murine respiratory infection model was used to study the mechanism of protective immunity to Bordetella pertussis. (tcd.ie)
Although we do not rule out a contribution of mucosal immunoglobulin A, our findings suggest that cellular responses mediated by CD4+ Th1 cells play an important role in protective immunity to B. pertussis. (tcd.ie)

The hMAb HBp2, an IgM, reacted with untreated and proteinase K-treated B. pertussis outer membrane antigens, whereas the reactivity was lost when the antigen was treated with sodium periodate. (nih.gov)
A 96-well plate has been precoated with Bordetella pertussis antigens to bind cognate antibodies. (abcam.com)
B. pertussis invasion of SKmel-23 cells was inhibited by preincubation of cancer cells with glycosidase F, the plant lectins LPHA and TGP, human antibodies vs CD15 and CD11b, or the RGD tripeptide sequence-implying that the structural requirements for invasion included β1,6-branched N-glycans, Lewis × antigens, CD11b, and the sequence arg-gly-asp respectivelly. (aacrjournals.org)
Circulating mononuclear cells from B. pertussis -infected and from pertussis-vaccinated infants secrete high amounts of IFN-γ after in vitro stimulation by B. pertussis antigens, but with a large variation in the secreted IFN-γ levels between individuals. (asm.org)
T lymphocytes from both naturally infected children and from vaccinated subjects secrete IFN-γ in response to in vitro stimulation with B. pertussis antigens ( 15 , 16 , 22 ). (asm.org)
Therefore, we investigated here whether IL-10 secretion in infants vaccinated with an acellular vaccine against B. pertussis plays a role in the high interindividual variations of the specific IFN-γ responses to B. pertussis antigens. (asm.org)

Helical structure of Bordetella pertussis fimbriae. (asm.org)
The helical structures of Bordetella pertussis fimbriae of serotypes 2 and 6 were determined by optical diffraction analysis of electron micrographs of negatively stained paracrystalline bundles of purified fimbriae. (asm.org)
BPZE1 is a derivative of the B. pertussis strain Tohama I, which produces serotype 2 (Fim2) but not serotype 3 fimbriae (Fim3). (openrepository.com)
Fimbriae (Fim), also known as pili and agglutinogen, belong to bacterial adhesins which are expressed on the B. pertussis surface. (biomedcentral.com)

Infants below the age of 12 months have the highest incidence and mortality associated with pertussis. (canada.ca)
This study included 404 household contacts of 94 young infants (≤6 months of age) with laboratory-confirmed pertussis. (asm.org)
DAPTACEL is a vaccine indicated for active immunization against diphtheria, tetanus and pertussis as a five dose series in infants and children 6 weeks through 6 years of age (prior to 7 th birthday). (nih.gov)
This specific IFN-γ secretion is at least partially induced by interleukin-12 (IL-12), as suggested by the strong correlation between B. pertussis -specific IFN-γ and IL-12 secretion levels, both in infected and in vaccinated infants ( 15 , 16 ). (asm.org)
However, although most vaccinated infants develop specific Th1 responses, these responses are very heterogeneous, according to the type of pertussis vaccine, and they are sometimes lower than detectable levels in peripheral blood. (asm.org)
The susceptibility to and the severity of Bordetella pertussis infections in infants and children varies widely, suggesting that genetic differences between individuals influence the course of infection. (synbiosis.com)
Pertussis shows a significant mortality rate in infants and was one of the most frequent and severe disease in this group before the worldwide introduction of an immunisation programme in the 1950s. (fiocruz.br)
The most morbidity and mortality with pertussis infection is seen in infants too young to benefit from immunization. (asmscience.org)
2013. Characteristics of severe Bordetella pertussis infection among infants ≤90 days of age admitted to pediatric intensive care units-Southern California, September 2009-June 2011. (asmscience.org)
Severe pulmonary hypertension associated with shock and death in infants infected with Bordetella pertussis. (qxmd.com)
Pathology and pathogenesis of fatal Bordetella pertussis infection in infants. (qxmd.com)
Fatal pulmonary hypertension associated with pertussis in infants: does extracorporeal membrane oxygenation have a role? (qxmd.com)

The purified lymphocytosis promoting factor (LPF) from Bordetella pertussis was found to be a potent mitogen for peripheral blood lymphocytes (PBL) from normal adults as well as for cord blood lymphocytes. (eurekamag.com)
The in vitro effects of Bordetella pertussis lymphocytosis-promoting factor on murine lymphocytes. (rupress.org)
The mitogenic response of murine lymphocytes to the lymphocytosis-promoting factor of Bordetella pertussis has been shown to be due to activation of T cells. (rupress.org)

B. pertussis is of worldwide prevalence as it can exist as a noninvasive parasite of the respiratory tract of mammals, however, 20-40 million cases of pertussis, and 400,000 fatal cases, are reported each year Footnote 9 . (canada.ca)
In the United States, the rates of pertussis infection moderately increased between the years 1980 and 2000, although larger rate increases were observed in adolescent and adult cases of pertussis Footnote 2 , Footnote 9 . (canada.ca)
However pertussis continues to circulate in the communities and estimates from WHO suggest that in 2008 about 16 million cases of pertussis occurred worldwide, 95% of which were reported in developing countries [ 7 ]. (biomedcentral.com)
All 21 cases of pertussis among the group under 10 years of age were at least partially vaccinated. (cancerscreening.gov.au)

Sections were obtained from the lungs of mice receiving saline, an OVA-challenge, and an OVA-challenge and heat-killed B. pertussis (2 × 10 10 cells) 1 day before the first airway challenge. (nih.gov)
B. pertussis targeted A549 human lung carcinomas growing s.c. in nu / nu mice. (aacrjournals.org)
A 95-kDa protein-negative derivative of B. pertussis 18323 containing a deletion of vag-8 colonized mice as efficiently as the parent B. pertussis strain in a mouse aerosol model of pertussis. (asm.org)
Aerosol infection of mice with Bordetella pertussis. (asm.org)
Aerosol inhalation of Bordetella pertussis Tohama phase I resulted in a reproducible and uniform infection of mice (strain DDY or ICR). (asm.org)
Subsequent studies using a 30-min aerosol inoculation of ICR mice with 2 X 10(9) bacterial cells per ml showed: (i) B. pertussis cells reached a maximum of about 10(7) colony-forming units per lung 14 days after inhalation. (asm.org)
In addition, we identified a new effector function for IL-17, activating macrophage killing of B. pertussis , and this bactericidal activity was less efficient in macrophages from TLR4-defective mice. (jimmunol.org)
The effect of an i.p. injection of Bordetella pertussis on the primary humoral immune response in mice to the thymus-independent antigen SIII has been studied. (jimmunol.org)
In mice, recovery from infection is associated with the development of B. pertussis -specific Th1 cells, and the adoptive transfer of these Th1 cells from convalescent mice confers protection to the recipient mice ( 18 ). (asm.org)
Splenocytes from infected C3H/HeJ mice produced almost no interleukin-1β (IL-1β) and tumor necrosis factor alpha (TNF-α) upon ex vivo restimulation with B. pertussis compared to A/J mice and also showed a delayed gamma interferon (IFN-γ) production. (synbiosis.com)
Here we have demonstrated that B. pertussis respiratory infection of mice with targeted disruptions of the genes for the IFN-γ receptor resulted in an atypical disseminated disease which was lethal in a proportion of animals, and was characterized by pyogranulomatous inflammation and postnecrotic scarring in the livers, mesenteric lymph nodes and kidneys. (rupress.org)

Bacterial culture for diagnosing pertussis infection has high specificity but poor sensitivity and is slow. (asm.org)
This study described a pertussis outbreak caused by macrolide-resistant B. pertussis in a primary school and indicated that close contact of index case causes the bacterial transmission. (ovid.com)

Between the years 1998-2004, the incidence of pertussis as well as pertussis-related mortality declined significantly following introduction of the acellular pertussis vaccine (DTaP-IPV-Hib vaccine) in 1998 Footnote 7 . (canada.ca)
Extensive immunization of children reduced the incidence of serious disease and mortality caused by B. pertussis [ 7 ]. (biomedcentral.com)
From 1975 to 1985 a lower vaccine dose was used, which most likely lead to an increase in the incidence of pertussis between 1985 to 1987 [ 21 ]. (biomedcentral.com)
After restoring the vaccine to the normal dose, a lower incidence of pertussis was noted until 1996. (biomedcentral.com)
However, since 1996, the pertussis incidence has increased. (biomedcentral.com)

Since the introduction of the acellular pertussis vaccine, the number of reported pertussis cases has drastically decreased. (pubmedcentralcanada.ca)

In this Review, we describe the pathogenesis of this disease, with a focus on recent mechanistic insights into B. pertussis virulence-factor function. (nih.gov)

Depletion of NK cells results in disseminating lethal infection with Bordetella pertussis associated with a reduction of antigen-specific Th1 and enhancement of Th2, but not Tr1 cells. (openrepository.com)

Abcam's anti-Bordetella pertussis IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgG class antibodies against Bordetella pertussis in Human serum and plasma. (abcam.com)

The National Immunization Commission and the National Program for the Control of Vaccine-Preventable Diseases (Programa Nacional de Control de Enfermedades Inmunoprevenibles, ProNaCEI) updated the immunization policy in relation to Bordetella pertussis (BP) in 2009 in order to improve the control of this disease in accordance with international recommendations. (scielo.org.ar)
Thus pertussis remains a poorly controlled vaccine preventable diseases in the world. (biomedcentral.com)
In the 1990s, pertussis reemerged even in countries with highly vaccinated populations and it is currently the most prevalent vaccine-preventable disease, representing a public health threat even in developed countries (Hartzell & Blaylock 2014). (fiocruz.br)

The B. pertussis ASR contains primers and a FAM-labeled probe that is designed to detect a 103 bp region of the IS481 gene. (bio-medicine.org)
The S1 subunits of both species, when expressed in Escherichia coli, have the same ADP-ribosylating activity as the S1 subunit from B. pertussis, indicating that the mutations in the S1 gene described here do not affect its function. (asm.org)
All the 12 DNAs had the A2047G mutation of 23S rRNA gene of B. pertussis. (ovid.com)
A putative metallo-β-lactamase gene presenting all of the conserved zinc-binding motifs that characterise the catalytic site was identified, in addition to a multidrug efflux pump of the RND family that could confer resistance to erythromycin, which is the antibiotic of choice for treating pertussis disease. (fiocruz.br)
A 120 bp long fragment from the IS481 gene from B.pertussis is amplified with specific primers and detected with a R6G labeled hydrolysis probe. (roche.com)
The adenylate cyclase gene of Bordetella pertussis: Molecular cloning and transcriptional analysis. (openrepository.com)

SERION ELISA classic Bordetella pertussis IgG/IgM/IgA are quantitative and qualitative tests for the detection of human antibodies in serum or plasma against Bordetella pertussis. (qedbio.com)

Evaluation of paired sera from six children with culture-proven pertussis demonstrated that antiadherence activity appeared in serum during convalescence from disease. (asm.org)
Highly sensitive real-time PCR assays and single-serum pertussis serology have been developed to overcome these limitations, but there are few data available on the relative sensitivities and specificities of such assays for pertussis diagnosis. (asm.org)
Serological assays, using one or two serum samples, have been developed to improve the sensitivity of the pertussis diagnosis, but they have a lower specificity than culture ( 3 , 9 , 20 , 25 ). (asm.org)
Proliferation occurred in autologous plasma or fetal calf serum, regardless of previous exposure to pertussis infection or immunization. (eurekamag.com)
A binding between the IgA antibodies of the serum and the immobilized Bordetella antigen takes place. (rapidtest.com)

During 2008-2012, a such as high rates of fever and local reactions, and vari- large outbreak of pertussis occurred. (cdc.gov)
CONCLUSION: Testing patients with respiratory symptoms using mPCR can improve early diagnosis of pertussis, ensure proper treatment, and may help with outbreak control. (medworm.com)
A pertussis outbreak was studied in a primary school in Xi'an, China, in March 2016. (ovid.com)
ECDC is addressing the harmonisation and improvement of pertussis diagnosis for surveillance and outbreak detection/monitoring in order to assure quality and comparability of data. (europa.eu)
This report published in Communicable Diseases Intelligence Volume 24, No 12, December 2000 contains the findings of the investigation of an outbreak of pertussis in Western Australia in 1999. (cancerscreening.gov.au)
In late 1999, an outbreak of Bordetella pertussis occurred in a small town in North-West Western Australia. (cancerscreening.gov.au)
We undertook an investigation to describe the outbreak and to identify strategies to minimise the impact of future pertussis outbreaks in Australia. (cancerscreening.gov.au)
This paper describes the epidemiology of the outbreak and outlines strategies for minimising the impact of future pertussis epidemics. (cancerscreening.gov.au)
However, our data suggest that it is suitable for inclusion in molecular epidemiological studies of the B. pertussis population or as a tool in outbreak investigations. (microbiologyresearch.org)

Pertussis is caused by Bordetella pertussis , a Gram-negative, pleomorphic coccobacillus, which is an exclusive human pathogen. (biomedcentral.com)

The most specific tests for diagnosis of pertussis were single serology and ptxA -Pr PCR, and the most sensitive diagnostic tool was the combination of IS 481 PCR with single serology. (asm.org)
Culturing B. pertussis from clinical specimens is the "gold standard" for diagnosis of pertussis, although this remains an insensitive method ( 12 , 13 ). (pubmedcentralcanada.ca)

Here we report that B. pertussis uses similar mechanisms for invasion of a variety of human cancer cells in vitro , and exhibits potent, invasion-dependent, cytotoxicity. (aacrjournals.org)
In a 30′ in vitro invasion assay, B. pertussis invaded human SKmel-23 melanoma cells at 20-30 times the rate for normal human melanocytes and fibroblasts. (aacrjournals.org)
Primary immunization with B. pertussis was also shown to generate lymph node cells which responded in vitro to secondary challenge with B. pertussis cells, FHA or PT. (eurekamag.com)

Is leukocytosis a predictor of mortality in severe pertussis infection? (qxmd.com)

In this study, we investigated whether whole-cell B. pertussis could inhibit allergic airway reactions in a murine model of asthma induced by ovalbumin (OVA). (nih.gov)
This study was performed to evaluate the immunogenicity, safety, and protection efficacy against Bordetella pertussis of the new Tdap vaccine in a murine model. (biomedcentral.com)
Intranasal murine model of Bordetella pertussis infection: II. (microbiologyresearch.org)

The causative agent of pertussis was identified and isolated by Jules Bordet and Octave Gengou in 1906. (wikipedia.org)

They also provided nasopharyngeal aspirates or, in a few centers, swab samples for culture and/or PCR detection of Bordetella pertussis and an acute blood sample for detection of anti-PT immunoglobulin G (IgG). (asm.org)
However, the rate of detection of B. pertussis by this medium was lower than that achieved with other conventional media ( 10 ). (pubmedcentralcanada.ca)
Providing a result for the detection of respiratory viruses, Mycoplasma, Chlamydophila & Pertussis in the Medical Admission when the patient is first seen, using point of care testing may avoid the issues highlighted, provide better quality of care, allow for better use of resources and reduce inappropriate antibiotic use and the development of antibiotic resistance. (isrctn.com)

What are the organism-specific therapeutic regimens for Bordetella pertussis bronchitis? (medscape.com)
Bordetella pertussis is BSL2 organism and should be considered a potential pathogen. (thelabrat.com)
B. pertussis has a stronger affinity for Dacron™ than for plain cotton wool or for treated cotton wool and its use improves recovery of the organism. (cmft.nhs.uk)
Concurrent culturing is used to differentiate species from B. pertussis and confirm the organism, while targeted DNA testing allows preliminary results to be reported the same day that the specimen is received. (loinc.org)
Our results show that B. pertussis is a dynamic organism that continues to evolve. (biomedcentral.com)

The data presented here provide support for CTB-Fim2 as a promising recombinant antigen against Bordetella pertussis infection. (biomedsearch.com)

The polymerase chain reaction (PCR) was recently added to conventional culture and serology for the diagnosis of Bordetella pertussis infection in a large vaccine efficacy trial in Germany. (ovid.com)

Effectiveness of rapid multiplex polymerase chain reaction for early diagnosis and treatment of pertussis. (medworm.com)
As part of this project ECDC is publishing two complementary guidance and protocols on Bordetella pertussis , the first on serological diagnosis of human infection and the second on the use of RT-PCR (real time polymerase chain reaction) for diagnosis of bordetella infections. (europa.eu)
On 26 November, B. pertussis was confirmed in both cases by polymerase chain reaction (PCR). (cancerscreening.gov.au)

Construction and evaluation of Bordetella pertussis live attenuated vaccine strain BPZE1 producing Fim3. (openrepository.com)
Is the Sequenced Bordetella pertussis strain Tohama I representative of the species? (pasteur.fr)
We conclude that Tohama I strain is not representative of the B. pertussis species. (pasteur.fr)

In the intranasal challenge test, inoculated B. pertussis was eradicated 7 days after infection. (biomedcentral.com)

Inflammation and infections as risk to prevent and control pertussis, but in many coun- factors for ischemic stroke. (cdc.gov)
2005. Molecular pathogenesis, epidemiology, and clinical manifestations of respiratory infections due to Bordetella pertussis and other Bordetella subspecies. (asmscience.org)

Bordet-Gengou agar (BG agar) was the standard culture medium for isolation of B. pertussis but has the problem of low selectivity. (pubmedcentralcanada.ca)

Bordetella is a Gram-negative, pleomorphic, aerobic coccobacillus. (asmscience.org)

Pertussis has shown a striking resurgence in the United States, with a return to record numbers of reported cases as last observed in the 1950s. (asm.org)
Numerous studies have demonstrated that the B. pertussis population has changed since the 1950s. (biomedcentral.com)

Anatomy5

Organisms6

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Chemicals and Drugs29

Analytical, Diagnostic and Therapeutic Techniques and Equipment5

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Health Care2

Role of antibodies against Bordetella pertussis virulence factors in adherence of Bordetella pertussis and Bordetella parapertussis to human bronchial epithelial cells. (1/1244)

Role of Bordetella pertussis virulence factors in adherence to epithelial cell lines derived from the human respiratory tract. (2/1244)

Characterization of human bactericidal antibodies to Bordetella pertussis. (3/1244)

Maternal immunization. (4/1244)

Temporal trends in the population structure of Bordetella pertussis during 1949-1996 in a highly vaccinated population. (5/1244)

Analysis with a combination of macrorestriction endonucleases reveals a high degree of polymorphism among Bordetella pertussis isolates in eastern France. (6/1244)

Serum IgG antibody responses to pertussis toxin and filamentous hemagglutinin in nonvaccinated and vaccinated children and adults with pertussis. (7/1244)

The conserved lysine 860 in the additional fatty-acylation site of Bordetella pertussis adenylate cyclase is crucial for toxin function independently of its acylation status. (8/1244)

Bordetella pertussis

Bordetella

Pertussis Vaccine

Bordetella Infections

Bordetella bronchiseptica

Whooping Cough

Virulence Factors, Bordetella

Pertussis Toxin

Bordetella parapertussis

Hemagglutinins

Adenylate Cyclase Toxin

Bordetella avium

Vaccines, Acellular

Antibodies, Bacterial

Adhesins, Bacterial

Adenylate Cyclase

Leukocytosis

Agglutinins

Bacterial Outer Membrane Proteins

Bacterial Proteins

Diphtheria-Tetanus-acellular Pertussis Vaccines

Diphtheria-Tetanus-Pertussis Vaccine

Nasopharynx

Antigens, Bacterial

Toxoids

Molecular Sequence Data

Genes, Bacterial

Virulence

Lymphocytosis

Gene Expression Regulation, Bacterial

DNA, Bacterial

Vaccination

Bacterial Adhesion

Siderophores

Mice, Inbred BALB C

Immunoglobulin G

Base Sequence

Immunization

Amino Acid Sequence

Agglutination Tests

Fimbriae, Bacterial

Diphtheria Toxoid

Trachea

Rhinitis, Atrophic

GTP-Binding Proteins

Bacterial Toxins

Adenosine Diphosphate Ribose

Respiratory System

Bacterial Vaccines

Hemolysin Proteins

Lung

Antitoxins

Cyclic AMP

Polymerase Chain Reaction

Enzyme-Linked Immunosorbent Assay

Immunoglobulin A

DNA Transposable Elements

Role of antibodies against Bordetella pertussis virulence factors in adherence of Bordetella pertussis and Bordetella parapertussis to human bronchial epithelial cells. (1/1244)

Role of Bordetella pertussis virulence factors in adherence to epithelial cell lines derived from the human respiratory tract. (2/1244)

Characterization of human bactericidal antibodies to Bordetella pertussis. (3/1244)

Maternal immunization. (4/1244)

Temporal trends in the population structure of Bordetella pertussis during 1949-1996 in a highly vaccinated population. (5/1244)

Analysis with a combination of macrorestriction endonucleases reveals a high degree of polymorphism among Bordetella pertussis isolates in eastern France. (6/1244)

Serum IgG antibody responses to pertussis toxin and filamentous hemagglutinin in nonvaccinated and vaccinated children and adults with pertussis. (7/1244)

The conserved lysine 860 in the additional fatty-acylation site of Bordetella pertussis adenylate cyclase is crucial for toxin function independently of its acylation status. (8/1244)

Bordetella pertussis adenylate cyclase. Penetration into host...

Permalien vers Bordetella pertussis adenylate cyclase toxin. Structural and functional independence of the catalytic and...

The Effect of Bordetella Pertussis on the Antibody Response in Mice to Type III Pneumococcal Polysaccharide | The Journal of...

Virulence of Pertactin-Negative Bordetella pertussis Isolates from Infants, France - Volume 19, Number 3-March 2013 - Emerging...

Changes in the genomic content of circulating Bordetella pertussis strains isolated from the Netherlands, Sweden, Japan and...

The mitogenic effect of the lymphocytosis promoting factor from Bordetella pertussis on human lymphocytes

Extracellular cAMP formation from host cell ATP by Bordetella Pertussis adenylate cyclase | IRIS Università degli Studi del...

Welcome to CDC stacks | Population Diversity among Bordetella pertussis Isolates, United States, 1935-2009 - 18731 | Emerging...

Rapid Increase in Pertactin-deficient Bordetella pertussis Isolates, Australia - Volume 20, Number 4-April 2014 - Emerging...

0288 Complete genome sequence of a clinical Bordetella pertussis isolate from Brazil

Surveillance of circulating bordetella pertussis strains in Europe during 1998 to 2015<...

Detection of anti-pertussis toxin IgG in oral fluids for use in diagnosis and surveillance of Bordetella pertussis infection in...

The effects of Bordetella pertussis on dendritic cell imprinting of CD by Sana Waheed

Intracellular survival of virulent Bordetella pertussis in human polymorphonuclear leukocytes<...

Complete Genome Sequence of Bordetella pertussis CS, a Chinese Pertussis Vaccine Strain | Journal of Bacteriology