Bordetella pertussis: A species of gram-negative, aerobic bacteria that is the causative agent of WHOOPING COUGH. Its cells are minute coccobacilli that are surrounded by a slime sheath.Bordetella: A genus of gram-negative, aerobic bacteria whose cells are minute coccobacilli. It consists of both parasitic and pathogenic species.Pertussis Vaccine: A suspension of killed Bordetella pertussis organisms, used for immunization against pertussis (WHOOPING COUGH). It is generally used in a mixture with diphtheria and tetanus toxoids (DTP). There is an acellular pertussis vaccine prepared from the purified antigenic components of Bordetella pertussis, which causes fewer adverse reactions than whole-cell vaccine and, like the whole-cell vaccine, is generally used in a mixture with diphtheria and tetanus toxoids. (From Dorland, 28th ed)Bordetella Infections: Infections with bacteria of the genus BORDETELLA.Bordetella bronchiseptica: A species of BORDETELLA that is parasitic and pathogenic. It is found in the respiratory tract of domestic and wild mammalian animals and can be transmitted from animals to man. It is a common cause of bronchopneumonia in lower animals.Whooping Cough: A respiratory infection caused by BORDETELLA PERTUSSIS and characterized by paroxysmal coughing ending in a prolonged crowing intake of breath.Virulence Factors, Bordetella: A set of BACTERIAL ADHESINS and TOXINS, BIOLOGICAL produced by BORDETELLA organisms that determine the pathogenesis of BORDETELLA INFECTIONS, such as WHOOPING COUGH. They include filamentous hemagglutinin; FIMBRIAE PROTEINS; pertactin; PERTUSSIS TOXIN; ADENYLATE CYCLASE TOXIN; dermonecrotic toxin; tracheal cytotoxin; Bordetella LIPOPOLYSACCHARIDES; and tracheal colonization factor.Pertussis Toxin: One of the virulence factors produced by BORDETELLA PERTUSSIS. It is a multimeric protein composed of five subunits S1 - S5. S1 contains mono ADPribose transferase activity.Bordetella parapertussis: A species of BORDETELLA with similar morphology to BORDETELLA PERTUSSIS, but growth is more rapid. It is found only in the RESPIRATORY TRACT of humans.Hemagglutinins: Agents that cause agglutination of red blood cells. They include antibodies, blood group antigens, lectins, autoimmune factors, bacterial, viral, or parasitic blood agglutinins, etc.Adenylate Cyclase Toxin: One of the virulence factors produced by virulent BORDETELLA organisms. It is a bifunctional protein with both ADENYLYL CYCLASES and hemolysin components.Bordetella avium: A species of BORDETELLA isolated from the respiratory tracts of TURKEYS and other BIRDS. It causes a highly contagious bordetellosis.Vaccines, Acellular: Vaccines that are produced by using only the antigenic part of the disease causing organism. They often require a "booster" every few years to maintain their effectiveness.Antibodies, Bacterial: Immunoglobulins produced in a response to BACTERIAL ANTIGENS.Adhesins, Bacterial: Cell-surface components or appendages of bacteria that facilitate adhesion (BACTERIAL ADHESION) to other cells or to inanimate surfaces. Most fimbriae (FIMBRIAE, BACTERIAL) of gram-negative bacteria function as adhesins, but in many cases it is a minor subunit protein at the tip of the fimbriae that is the actual adhesin. In gram-positive bacteria, a protein or polysaccharide surface layer serves as the specific adhesin. What is sometimes called polymeric adhesin (BIOFILMS) is distinct from protein adhesin.Adenylate Cyclase: An enzyme of the lyase class that catalyzes the formation of CYCLIC AMP and pyrophosphate from ATP. EC 4.6.1.1.Leukocytosis: A transient increase in the number of leukocytes in a body fluid.Agglutinins: Substances, usually of biological origin, that cause cells or other organic particles to aggregate and stick to each other. They include those ANTIBODIES which cause aggregation or agglutination of particulate or insoluble ANTIGENS.Bacterial Outer Membrane Proteins: Proteins isolated from the outer membrane of Gram-negative bacteria.Bacterial Proteins: Proteins found in any species of bacterium.Diphtheria-Tetanus-acellular Pertussis Vaccines: Combined vaccines consisting of DIPHTHERIA TOXOID; TETANUS TOXOID; and an acellular form of PERTUSSIS VACCINE. At least five different purified antigens of B. pertussis have been used in various combinations in these vaccines.Diphtheria-Tetanus-Pertussis Vaccine: A vaccine consisting of DIPHTHERIA TOXOID; TETANUS TOXOID; and whole-cell PERTUSSIS VACCINE. The vaccine protects against diphtheria, tetanus, and whooping cough.Nasopharynx: The top portion of the pharynx situated posterior to the nose and superior to the SOFT PALATE. The nasopharynx is the posterior extension of the nasal cavities and has a respiratory function.Antigens, Bacterial: Substances elaborated by bacteria that have antigenic activity.Toxoids: Preparations of pathogenic organisms or their derivatives made nontoxic and intended for active immunologic prophylaxis. They include deactivated toxins. Anatoxin toxoids are distinct from anatoxins that are TROPANES found in CYANOBACTERIA.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Genes, Bacterial: The functional hereditary units of BACTERIA.Virulence: The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.Lymphocytosis: Excess of normal lymphocytes in the blood or in any effusion.Gene Expression Regulation, Bacterial: Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Vaccination: Administration of vaccines to stimulate the host's immune response. This includes any preparation intended for active immunological prophylaxis.Bacterial Adhesion: Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity.Siderophores: Low-molecular-weight compounds produced by microorganisms that aid in the transport and sequestration of ferric iron. (The Encyclopedia of Molecular Biology, 1994)Mice, Inbred BALB CImmunoglobulin G: The major immunoglobulin isotype class in normal human serum. There are several isotype subclasses of IgG, for example, IgG1, IgG2A, and IgG2B.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Immunization: Deliberate stimulation of the host's immune response. ACTIVE IMMUNIZATION involves administration of ANTIGENS or IMMUNOLOGIC ADJUVANTS. PASSIVE IMMUNIZATION involves administration of IMMUNE SERA or LYMPHOCYTES or their extracts (e.g., transfer factor, immune RNA) or transplantation of immunocompetent cell producing tissue (thymus or bone marrow).Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Agglutination Tests: Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (From Stedman, 26th ed)Fimbriae, Bacterial: Thin, hairlike appendages, 1 to 20 microns in length and often occurring in large numbers, present on the cells of gram-negative bacteria, particularly Enterobacteriaceae and Neisseria. Unlike flagella, they do not possess motility, but being protein (pilin) in nature, they possess antigenic and hemagglutinating properties. They are of medical importance because some fimbriae mediate the attachment of bacteria to cells via adhesins (ADHESINS, BACTERIAL). Bacterial fimbriae refer to common pili, to be distinguished from the preferred use of "pili", which is confined to sex pili (PILI, SEX).Diphtheria Toxoid: The formaldehyde-inactivated toxin of Corynebacterium diphtheriae. It is generally used in mixtures with TETANUS TOXOID and PERTUSSIS VACCINE; (DTP); or with tetanus toxoid alone (DT for pediatric use and Td, which contains 5- to 10-fold less diphtheria toxoid, for other use). Diphtheria toxoid is used for the prevention of diphtheria; DIPHTHERIA ANTITOXIN is for treatment.Trachea: The cartilaginous and membranous tube descending from the larynx and branching into the right and left main bronchi.Rhinitis, Atrophic: A chronic inflammation in which the NASAL MUCOSA gradually changes from a functional to a non-functional lining without mucociliary clearance. It is often accompanied by degradation of the bony TURBINATES, and the foul-smelling mucus which forms a greenish crust (ozena).GTP-Binding Proteins: Regulatory proteins that act as molecular switches. They control a wide range of biological processes including: receptor signaling, intracellular signal transduction pathways, and protein synthesis. Their activity is regulated by factors that control their ability to bind to and hydrolyze GTP to GDP. EC 3.6.1.-.Bacterial Toxins: Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.Adenosine Diphosphate Ribose: An ester formed between the aldehydic carbon of RIBOSE and the terminal phosphate of ADENOSINE DIPHOSPHATE. It is produced by the hydrolysis of nicotinamide-adenine dinucleotide (NAD) by a variety of enzymes, some of which transfer an ADP-ribosyl group to target proteins.Respiratory System: The tubular and cavernous organs and structures, by means of which pulmonary ventilation and gas exchange between ambient air and the blood are brought about.Bacterial Vaccines: Suspensions of attenuated or killed bacteria administered for the prevention or treatment of infectious bacterial disease.Hemolysin Proteins: Proteins from BACTERIA and FUNGI that are soluble enough to be secreted to target ERYTHROCYTES and insert into the membrane to form beta-barrel pores. Biosynthesis may be regulated by HEMOLYSIN FACTORS.Lung: Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.Antitoxins: Antisera from immunized animals that is purified and used as a passive immunizing agent against specific BACTERIAL TOXINS.Cyclic AMP: An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Enzyme-Linked Immunosorbent Assay: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.Immunoglobulin A: Represents 15-20% of the human serum immunoglobulins, mostly as the 4-chain polymer in humans or dimer in other mammals. Secretory IgA (IMMUNOGLOBULIN A, SECRETORY) is the main immunoglobulin in secretions.DNA Transposable Elements: Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.

Role of antibodies against Bordetella pertussis virulence factors in adherence of Bordetella pertussis and Bordetella parapertussis to human bronchial epithelial cells. (1/1244)

Immunization with whole-cell pertussis vaccines (WCV) containing heat-killed Bordetella pertussis cells and with acellular vaccines containing genetically or chemically detoxified pertussis toxin (PT) in combination with filamentous hemagglutinin (FHA), pertactin (Prn), or fimbriae confers protection in humans and animals against B. pertussis infection. In an earlier study we demonstrated that FHA is involved in the adherence of these bacteria to human bronchial epithelial cells. In the present study we investigated whether mouse antibodies directed against B. pertussis FHA, PTg, Prn, and fimbriae, or against two other surface molecules, lipopolysaccharide (LPS) and the 40-kDa outer membrane porin protein (OMP), that are not involved in bacterial adherence, were able to block adherence of B. pertussis and B. parapertussis to human bronchial epithelial cells. All antibodies studied inhibited the adherence of B. pertussis to these epithelial cells and were equally effective in this respect. Only antibodies against LPS and 40-kDa OMP affected the adherence of B. parapertussis to epithelial cells. We conclude that antibodies which recognize surface structures on B. pertussis or on B. parapertussis can inhibit adherence of the bacteria to bronchial epithelial cells, irrespective whether these structures play a role in adherence of the bacteria to these cells.  (+info)

Role of Bordetella pertussis virulence factors in adherence to epithelial cell lines derived from the human respiratory tract. (2/1244)

During colonization of the respiratory tract by Bordetella pertussis, virulence factors contribute to adherence of the bacterium to the respiratory tract epithelium. In the present study, we examined the roles of the virulence factors filamentous hemagglutinin (FHA), fimbriae, pertactin (Prn), and pertussis toxin (PT) in the adherence of B. pertussis to cells of the human bronchial epithelial cell line NCI-H292 and of the laryngeal epithelial cell line HEp-2. Using B. pertussis mutant strains and purified FHA, fimbriae, Prn, and PT, we demonstrated that both fimbriae and FHA are involved in the adhesion of B. pertussis to laryngeal epithelial cells, whereas only FHA is involved in the adherence to bronchial epithelial cells. For PT and Prn, no role as adhesion factor was found. However, purified PT bound to both bronchial and laryngeal cells and as such reduced the adherence of B. pertussis to these cells. These data may imply that fimbriae play a role in infection of only the laryngeal mucosa, while FHA is the major factor in colonization of the entire respiratory tract.  (+info)

Characterization of human bactericidal antibodies to Bordetella pertussis. (3/1244)

The Bordetella pertussis BrkA protein protects against the bactericidal activity of complement and antibody; however, some individuals mount an immune response that overcomes this bacterial defense. To further characterize this process, the bactericidal activities of sera from 13 adults with different modes of exposure to B. pertussis (infected as adults, occupational exposure, immunized with an acellular vaccine, or no identified exposure) against a wild-type strain and a BrkA complement-sensitive mutant were evaluated. All of the sera killed the BrkA mutant, suggesting past exposure to B. pertussis or cross-reactive organisms. Several samples had no or minimal activity against the wild type. All of the sera collected from the infected and occupationally exposed individuals but not all of the sera from vaccinated individuals had bactericidal activity against the wild-type strain, suggesting that some types of exposure can induce an immune response that can overcome the BrkA resistance mechanism. Adsorbing serum with the wild-type strain removed the bactericidal antibodies; however, adsorbing the serum with a lipopolysaccharide (LPS) mutant or an avirulent (bvg mutant) strain did not always result in loss of bactericidal activity, suggesting that antibodies to either LPS or bvg-regulated proteins could be bactericidal. All the samples, including those that lacked bactericidal activity, contained antibodies that recognized the LPS of B. pertussis. Bactericidal activity correlated best with the presence of the immunoglobulin G3 (IgG3) antibodies to LPS, the IgG subtype that is most effective at fixing complement.  (+info)

Maternal immunization. (4/1244)

Maternal immunization can enhance passive immunity of infants to pathogens that cause life-threatening illnesses. In most instances, immunization during pregnancy will provide important protection for the woman as well as for her offspring. The tetanus toxoid and influenza vaccines are examples of vaccines that provide a double benefit. Other vaccines under evaluation include those for respiratory syncytial virus, pneumococci, group B streptococci, and Haemophilus influenzae type b. Although most IgG antibody crosses the placenta in the third trimester, the process is time-dependent, dictating that immunization should be accomplished ideally at least 6 weeks prior to delivery. IgG1 antibodies are transferred preferentially. Maternal immunization has not interfered with active immunization of the infant. Inactivated vaccines administered in the third trimester of pregnancy pose no known risk to the woman or to her fetus.  (+info)

Temporal trends in the population structure of Bordetella pertussis during 1949-1996 in a highly vaccinated population. (5/1244)

The population structure of Bordetella pertussis in The Netherlands in 5 successive periods, encompassing 1949-1996, was analyzed by DNA typing ("fingerprinting"). In 10 years following the introduction of wide-scale vaccination in 1953, a decrease in genotypic diversity (GD) was observed, suggesting clonal expansion of strains that were adapted to vaccine-induced immunity. In subsequent periods, GD increased to prevaccination levels, probably reflecting a gradual adaptation of the B. pertussis population involving many lineages. In the 1990s, GD decreased again. This decrease coincided with an antigenic shift in the surface protein pertactin. No evidence was found for changes in DNA types or GD in 1996, when a large pertussis epidemic occurred. Thus, gradual changes in the bacterial population previous to 1996 were probably the cause of the 1996 epidemic. The results herein suggest that vaccination has selected for strains that are adapted to a highly vaccinated population. Similar changes may have occurred in other countries, explaining the reemergence of pertussis in vaccinated populations.  (+info)

Analysis with a combination of macrorestriction endonucleases reveals a high degree of polymorphism among Bordetella pertussis isolates in eastern France. (6/1244)

From 1990 to 1996, routine screening for whooping cough identified 399 patients with a calmodulin-dependent adenylate cyclase-positive test result and yielded 69 Bordetella pertussis isolates. None of the patients were fully vaccinated, and most were less than 6 months old. Analysis of total DNA by pulsed-field gel electrophoresis (PFGE) after XbaI, SpeI, or DraI macrorestriction yielded 19, 15, and 5 different patterns, respectively, whereas ribotyping failed to demonstrate any strain polymorphism. Discrimination among the isolates was improved by combining the PFGE profiles. Some patterns were more frequent, but the corresponding patients were not clearly epidemiologically related. The patterns for two strains obtained during a 3-month period from patients who were neighbors differed by the length of a single DNA fragment. These data strongly suggest that one type of isolate is widely spread throughout the world and is carried by individuals other than patients who develop a true illness.  (+info)

Serum IgG antibody responses to pertussis toxin and filamentous hemagglutinin in nonvaccinated and vaccinated children and adults with pertussis. (7/1244)

Levels of IgG antibody to pertussis toxin (PT) and filamentous hemagglutinin (FHA) were measured in paired serum samples from 781 patients fulfilling at least one laboratory criterion for pertussis that was suggested by an ad hoc committee sponsored by the World Health Organization. The patients were participants or family members of participants in a double-blind efficacy trial of a monocomponent pertussis toxoid vaccine. Of 596 nonvaccinated children, 90% had significant (two-fold or more) rises in PT IgG and FHA IgG levels. Only 17 (32%) of 53 children previously vaccinated with three doses of pertussis toxoid had rises in PT IgG levels because they already had elevated PT IgG levels in their acute-phase serum samples. PT IgG and FHA IgG levels were significantly higher in acute-phase serum samples from 29 adults than in acute-phase serum samples from the nonvaccinated children. Nevertheless, significant rises in levels of PT IgG (79% of samples) and FHA IgG (90%) were demonstrated in adults. In conclusion, assay of PT IgG and FHA IgG in paired serum samples is highly sensitive for diagnosing pertussis in nonvaccinated individuals. Assay of PT IgG levels in paired sera is significantly less sensitive for diagnosis of pertussis for children vaccinated with pertussis toxoid.  (+info)

The conserved lysine 860 in the additional fatty-acylation site of Bordetella pertussis adenylate cyclase is crucial for toxin function independently of its acylation status. (8/1244)

The Bordetella pertussis RTX (repeat in toxin family protein) adenylate cyclase toxin-hemolysin (ACT) acquires biological activity upon a single amide-linked palmitoylation of the epsilon-amino group of lysine 983 (Lys983) by the accessory fatty-acyltransferase CyaC. However, an additional conserved RTX acylation site can be identified in ACT at lysine 860 (Lys860), and this residue becomes palmitoylated when recombinant ACT (r-Ec-ACT) is produced together with CyaC in Escherichia coli K12. We have eliminated this additional acylation site by replacing Lys860 of ACT with arginine, leucine, and cysteine residues. Two-dimensional gel electrophoresis and microcapillary high performance liquid chromatography/tandem mass spectrometric analyses of mutant proteins confirmed that the two sites are acylated independently in vivo and that mutations of Lys860 did not affect the quantitative acylation of Lys983 by palmitoyl (C16:0) and palmitoleil (cis Delta9 C16:1) fatty-acyl groups. Nevertheless, even the most conservative substitution of lysine 860 by an arginine residue caused a 10-fold decrease of toxin activity. This resulted from a 5-fold reduction of cell association capacity and a further 2-fold reduction in cell penetration efficiency of the membrane-bound K860R toxin. These results suggest that lysine 860 plays by itself a crucial structural role in membrane insertion and translocation of the toxin, independently of its acylation status.  (+info)

Bordetella pertussis adenylate cyclase. Penetration into host cells.: Exposure of Chinese hamster ovary, mouse adrenal cortex tumor (Y-1), THP-1 and U-937 cells
The effect of an i.p. injection of Bordetella pertussis on the primary humoral immune response in mice to the thymus-independent antigen SIII has been studied. Suppression of the antibody response occurred when pertussis cells were injected at the same time as an optimal immunizing dose of SIII. In contrast, the antibody response to high doses of SIII was enhanced by B. pertussis.. When SIII alone was injected, only 19S antibody was detected. However, when B. pertussis was administered with either optimal or high doses of SIII, 7S as well as 19S antibody against SIII was produced.. ...
Bordetella pertussis isolates that do not express pertactin (PRN) are increasing in regions where acellular pertussis vaccines have been used for >7 years. We analyzed data from France and compared clinical symptoms among infants <6 months old infected by PRN-positive or PRN-negative isolates. No major clinical differences were found between the 2 groups.
Bordetella pertussis is the causative agent of human whooping cough (pertussis) and is particularly severe in infants. Despite worldwide vaccinations, whooping cough remains a public health problem. A significant increase in the incidence of whooping cough has been observed in many countries since the 1990s. Several reasons for the re-emergence of this highly contagious disease have been suggested. A particularly intriguing possibility is based on evidence indicating that pathogen adaptation may play a role in this process. In an attempt to gain insight into the genomic make-up of B. pertussis over the last 60 years, we used an oligonucleotide DNA microarray to compare the genomic contents of a collection of 171 strains of B. pertussis isolates from different countries. The CGH microarray analysis estimated the core genome of B. pertussis, to consist of 3,281 CDSs that are conserved among all B. pertussis strains, and represent 84.8% of all CDSs found in the 171 B. pertussis strains. A total of 64
Morse, J.H.; Kong, A.S.; Lindenbaum, J.; Morse, S.I., 1977: The mitogenic effect of the lymphocytosis promoting factor from Bordetella pertussis on human lymphocytes
The effect of exogenously added adenylate cyclase from Bordetella pertussis (strain 114) has been investigated in Y-1 mouse adrenal tumor, chinese hamster ovary (CHO) and several other cells. A partially purified adenylate cyclase was found not to enter cells but, nevertheless, produced large amounts of cAMP in the medium. We could show that this resulted from release of ATP (and not larger molecules). The ATP released by the cells could be (1) directly measured and was replenished after each change of medium; (2) was reciprocally related to the cAMP produced; and (3) was competed for by ATPases present in added serum or by hexokinase and, less effectively, by exoenzymes on the cell surface. The extent of ATP leakage varied widely between different cell lines, being marked in CHO and Y-1 adrenal cells but negligible in transformed lymphocyte lines. The uncertainty of the origin of cAMP found in media of cultured cells requires separate analysis of cell and medium cAMP and an assessment of ATP ...
Since the 1980s, pertussis notifications in the United States have been increasing. To determine the types of Bordetella pertussis responsible for these increases, we divided 661 B. pertussis isolates collected in the United States during 1935-2009 into 8 periods related to the introduction of novel vaccines or changes in vaccination schedule. B. pertussis diversity was highest from 1970-1990 (94%) but declined to ≈ 70% after 1991 and has remained constant. During 2006-2009, 81.6% of the strains encoded multilocus sequence type prn2-ptxP3-ptxS1A-fim3B, and 64% were multilocus variable number tandem repeat analysis type 27. US trends were consistent with those seen internationally; emergence and predominance of the fim3B allele was the only molecular characteristic associated with the increase in pertussis notifications. Changes in the vaccine composition and schedule were not the direct selection pressures that resulted in the allele changes present in the current B. pertussis population ...
Acellular vaccines against Bordetella pertussis were introduced in Australia in 1997. By 2000, these vaccines had replaced whole-cell vaccines. During 2008-2012, a large outbreak of pertussis occurred. During this period, 30% (96/320) of B. pertussis isolates did not express the vaccine antigen pertactin (prn). Multiple mechanisms of prn inactivation were documented, including IS481 and IS1002 disruptions, a variation within a homopolymeric tract, and deletion of the prn gene. The mechanism of lack of expression of prn in 16 (17%) isolates could not be determined at the sequence level. These findings suggest that B. pertussis not expressing prn arose independently multiple times since 2008, rather than by expansion of a single prn-negative clone. All but 1 isolate had ptxA1, prn2, and ptxP3, the alleles representative of currently circulating strains in Australia. This pattern is consistent with continuing evolution of B. pertussis in response to vaccine selection pressure.
Bordetella pertussis infection is being increasingly recognized as a cause of prolonged, distressing cough (without whooping symptoms) in children and young adults. Diagnosis of infection in this population is important for treatment and surveillance purposes, and may also prove useful in reducing transmission to unvaccinated babies, for whom disease can be fatal. Serum IgG titres against pertussis toxin (PT) are routinely used as a marker of recent or persisting B. pertussis infection. However, collection of serum from young children is difficult, and compliance amongst these subjects to give samples is low. To circumvent these problems, an IgG-capture ELISA capable of detecting anti-PT IgG in oral fluid was devised. The assay was evaluated by comparison to a serum ELISA, using 187 matched serum and oral fluid samples from children (aged 5-16 years) with a history of prolonged coughing, whose serum anti-PT titre had already been determined (69 seropositive, 118 seronegative). The results showed that,
Bordetella pertussis is an aerobic gram-negative bacterial pathogen that causes the human respiratory disease whooping cough. Despite widespread vaccination, whooping cough is reemerging due to decreased vaccine efficacy. One of the hallmarks of infection is lymphocytosis, which is induced by the pertussis toxin. Lymphocytes such as CD4+ T cells navigate to infected tissues through surface-trafficking molecules, which are imprinted during their interaction with tissue-associated dendritic cells. We hypothesized that the pertussis toxin affects the imprinting process resulting in altered expression of trafficking molecules on CD4+ T cells. We tested this hypothesis using a mouse model of infection. Imprinting levels on CD4+ T cells were compared to Bordetella parapertussis, a related strain that lacks pertussis toxin. Our results indicated that 5 days post-infection, the percentage of lung dendritic cells increased and adopted a mature phenotype (displaying an increased capability to migrate and present
Bordetella pertussis is a strict human pathogen that is the causative agent of pertussis (whooping cough). Despite widespread immunization with pertussis vaccines, many countries still report outbreaks (1, 12, 14). Resurgence of pertussis has been recently observed in some countries with high vaccination coverage (4, 8, 10). It is suggested that pathogen adaptation may play a role in the reemergence of pertussis (10, 15, 16). In this present work, the complete genome sequence of B. pertussis strain CS, which was isolated from an infant patient in 1951 in Beijing and widely used as a vaccine strain for production of an acellular pertussis vaccine in China, was determined and compared with the published genome of Tohama I (11).. Whole-genome sequencing of B. pertussis CS was performed with a combined strategy of the Sanger shotgun approach (6) and 454 fragment sequencing technology (9). A total of 329,480 reads, giving 28-fold coverage of the genome, were generated using the GS FLX system (454 ...
Bordetella pertussis ATCC ® BAA-589D-5™ Designation: Genomic DNA from Bordetella pertussis Strain Tohama 1 TypeStrain=False Application:
The Global and Chinese Bordetella Pertussis Industry, 2011-2021 Market Research Report is a professional and in-depth study on the current state of the global Bordetella Pertussis industry with a focus on the Chinese market. The report provides key statistics on the market status of the Bordetella Pertussis manufacturers and is a valuable source of guidance and direction for companies and individuals interested in the industry.. Complete report of 150 pages published in Jan 2016 is available at http://www.market-research-reports.com/434778-bordetella-pertussis-industry.. Firstly, the report provides a basic overview of the industry including its definition, applications and manufacturing technology. Then, the report explores the international and Chinese major industry players in detail. In this part, the report presents the company profile, product specifications, capacity, production value, and 2011-2016 market shares for each company. Through the statistical analysis, the report depicts the ...
BACKGROUND: Each year, Bordetella pertussis infection causes an estimated 294,000 deaths worldwide, primarily among young, nonvaccinated children. Approximately 90% of all deaths due to pertussis in the Unites States occur in young infants. These children often develop intractable pulmonary hypertension; however, the pathophysiologic mechanism responsible for this complication has not been well characterized, and there have been no detailed descriptions of the pathology of this disease since the 1940s.. METHODS: Respiratory tissue samples obtained at autopsy from 15 infants aged ,or=4 months who had polymerase chain reaction- or culture-confirmed B. pertussis pneumonia were evaluated by multiple histochemical stains, immunohistochemical evaluation, and electron microscopic examination.. RESULTS: The pulmonary histopathologic examination of the samples revealed a descending infection dominated by necrotizing bronchiolitis, intra-alveolar hemorrhage, and fibrinous edema. All samples had marked ...
Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S.
In order to achieve batch-to-batch consistency of whole-cell pertussis vaccines, properties relevant for protection and safety should be characterised. Therefore, ELISAs to quantify pertussis toxin (PT), filamentous haemagglutinin (FHA), 92 kD outer membrane protein (92 kD-OMP) and pertactin (PRN) in Bordetella pertussis (B. pertussis) suspensions were developed. In this paper the influence of the bacterial growth stage on antigen production and antigen release into the supernatant was studied for pertussis strains 134, 509 and CS. The levels of cell-associated and free antigens during growth were strongly strain and antigen dependent. Because of this, the proportion of cell-associated antigens changed during cultivation for all three strains. Substantial amounts of PT and PRN were released into the supernatant, while little free FHA and 92 kD-OMP were found. The amount of cell-associated FHA declined rapidly during growth, whereas cell-associated 92 kD-OMP contents increased. These findings ...
OBJECTIVE: To study the clinical presentation of culture-confirmed pertussis in children and their contacts with cough illnesses in an outpatient setting. METHODOLOGY: In conjunction with a large pertussis vaccine efficacy trial in Germany, a central laboratory to isolate Bordetella species from nasopharyngeal specimens was established in Erlangen in October 1990. Pediatricians in private practices in southern Germany, the Saar region, and Berlin were encouraged to obtain nasopharyngeal specimens and clinical characteristics from patients with cough illnesses ,/=7 days duration. Bordetella species were isolated by use of calcium alginate swabs, Regan-Lowe agar, and modified Stainer-Scholte broth. Clinical characteristics were determined by initial and follow-up questionnaires. RESULTS: From October 1990 to September 1996, 20 972 specimens were submitted, and B pertussis was isolated in 2592 instances (12.4%). Of the culture-proven cases, 50.7% were female, and the age range was 6 days to 41 ...
Background. Acellular pertussis (aP) booster immunizations have been recommended for adolescents and older persons to enhance long-term protection and to possibly reduce community transmission of infections.. Methods. This was a multicenter, randomized, double-blind vaccine trial in which one-half of the subjects received aP vaccine and one-half received hepatitis A vaccine (control subjects). All subjects were observed for almost 2 years for cough illnesses, and all underwent microbiologic and serologic studies for detection of pertussis infection. Immunoglobulin G (IgG) and immunoglobulin A (IgA) antibodies to pertussis toxin, filamentous hemagglutinin, pertactin, and fimbriae 2/3 were measured by enzyme-linked immunosorbent assay in serum samples obtained 1 and 12 months after immunization. Infection rates were determined with a variety of serologic criteria for control and vaccinated subjects. The incidence of prolonged cough illness was ascertained for subjects with and subjects without ...
GENTILE, Ángela et al. Cost of Bordetella pertussis illness in tertiary hospitals in Argentina. Arch. argent. pediatr. [online]. 2013, vol.111, n.4, pp.295-302. ISSN 0325-0075. http://dx.doi.org/10.5546/aap.2013.295.. The National Immunization Commission and the National Program for the Control of Vaccine-Preventable Diseases (Programa Nacional de Control de Enfermedades Inmunoprevenibles, ProNaCEI) updated the immunization policy in relation to Bordetella pertussis (BP) in 2009 in order to improve the control of this disease in accordance with international recommendations. To evaluate the fnancial impact of this new immunization policy, we must frst know the cost on the health system of having a hospitalized or outpatient child infected with BP. The objective of this study was to describe the profle of costs of hospitalized or outpatient children with laboratory-confrmed BP infection in three hospitals of Argentina. This was a prospective study of the cost of BP in the period between December ...
ID BOPER1_1_PE1000 STANDARD; PRT; 266 AA. AC BOPER1_1_PE1000; Q7VZ03; DT 00-JAN-0000 (Rel. 1, Created) DT 00-JAN-0000 (Rel. 2, Last sequence update) DT 00-JAN-0000 (Rel. 3, Last annotation update) DE SubName: Full=Competence lipoprotein; (BOPER1_1.PE1000). GN Name=comL; OrderedLocusNames=BP1146; OS BORDETELLA PERTUSSIS TOHAMA I. OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; OC Alcaligenaceae; Bordetella. OX NCBI_TaxID=257313; RN [0] RP -.; RG -.; RL -.; CC -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS BOPER1_1.PE1000. CC Bordetella pertussis Tohama I, complete genome. CC complete sequence. CC -!- ANNOTATIONS ORIGIN:Q7VZ03_BORPE CC -!- GENE_FAMILY: HOG000260923 [ FAMILY / ALN / TREE ] DR UniProtKB/Swiss-Prot; Q7VZ03; -. DR EMBL; BX640414; CAE41443.1; -; Genomic_DNA. DR RefSeq; NP_879922.1; NC_002929.2. DR GeneID; 2665391; -. DR GenomeReviews; BX470248_GR; BP1146. DR KEGG; bpe:BP1146; -. DR OMA; IHVADYY; -. DR PhylomeDB; Q7VZ03; -. DR ProtClustDB; CLSK920261; -. DR GO; ...
What is pertussis (whooping cough)? Pertussis (also called whooping cough) is a disease caused by the bacteria Bordetella pertussis that spreads from person-to-person with close contact. It may cause severe coughing fits which can affect breathing. Pertussis is often milder in older children and adults, but can cause serious problems in infants. Pertussis can lead to pneumonia, convulsions, inflammation of the brain and sometimes death. Most of these serious problems occur in infants who are less than one year old. Who can get pertussis? Pertussis can occur in any age group; however, pertussis is more common among infants since they are too young to have full protection from the vaccine. Pertussis is also more common in adolescents and adults who have lost the protection they got from vaccination or illness in childhood. How is pertussis spread? Pertussis is spread from one person to another through respiratory droplets from the nose or throat of an infected person by coughing or sneezing, and ...
Mooi FR; van Loo IHM; van Gent M; He Q; Bart MJ; Heuvelman KJ; de Greeff SC; Diavatopoulos D; Teunis P; Nagelkerke N; Mertsola J (2009 ...
... ,ARUP Laboratories is a national reference laboratory and a worldwide leader in innovative laboratory research and development. ARUP offers an extensive test menu of highly complex and unique medical tests in clinical and anatomic pathology. Owned by the University of Utah, ARUP Laboratories client,medicine,medical supply,medical supplies,medical product
Despite the availability of highly effective vaccines, Bordetella pertussis incidence has been rapidly rising in highly vaccinated populations. Recent outbreaks have received media attention, feeding concerns about the emergence of dangerous new strains with increased virulence or that escape vaccine-induced immunity. To accelerate the study of this reemerging pathogen, we sequenced the genomes of 28 B. pertussis strains isolated during outbreaks from 2010 through 2012, making both strains and sequence data available to the scientific community ...
IDENTIFICATION AND USE: a simple chemically defined medium for the production of phase 1 Bordetella Pertussis described consisting of sodium glutamate, proline, cystine, salts, and growth factors, which is suitable for the large-scale production of phase 1 bordetella pertussis. The cultures were detoxified by the addition of 0.14% formalin. The acellular pertussis antigens (PT, FHA, and pertactin) are isolated from Bordetella pertussis culture grown in modified Stainer-Scholte liquid medium. PT and FHA are isolated from the fermentation broth; pertactin is extracted from the cells by heat treatment and flocculation. The antigens are purified in successive chromatographic and precipitation steps. PT is detoxified using glutaraldehyde and formaldehyde. FHA and pertactin are treated with formaldehyde. Each antigen is individually adsorbed onto aluminum hydroxide. Each 0.5-mL dose is formulated to contain 5 Lf of tetanus toxoid, 2.5 Lf of diphtheria toxoid, 8 mcg of inactivated PT, 8 mcg of FHA, and ...
Bordetella pertussis, the human pathogen of whooping cough, when grown at 22 degrees C is nonvirulent and unable to bind eukaryotic cells. In response to a temperature shift to 37 degrees C, the bacterium acquires the ability to bind eukaryotic cells in a time-dependent fashion. By studying in vitro the temperature-induced transition, from the nonvirulent to the virulent state, we found that binding to CHO cells is mediated by the Arg-Gly-Asp-containing domain of filamentous hemagglutinin (FHA), a protein with multiple binding specificities. This protein is synthesized as a 367-kDa polypeptide within 10 min after temperature shift, but requires 2 hr before it is detected on the bacterial cell surface and starts to bind CHO cells. Mutations affecting the cell surface export of FHA abolish bacterial adhesion to CHO cells, while mutations in the outer membrane protein pertactin strongly reduce binding. This suggests that multiple chaperon proteins are required for a correct function of FHA. ...
... ,The B. pertussis ASR contains primers and a FAM-labeled probe that is designed to detect a 103 bp region of the IS481 gene. In addition, the B. pertussis ASR contains primers, a Texas Red-labeled probe and DNA for an internal control sequence. This ASR requires an instrument that can detect FAM and,medicine,medical supply,medical supplies,medical product
Pertussis (whooping cough) is a disease of uncontrollable coughing as a result of infection caused by bacteria Bordetella pertussis Types of food to prevent and treat Pertussis 1. Green tea and black tea In the study to evaluate the efficacy of anti bactericidal activity of tea and catechins against Bordetella pertussis, indicated that pu-erh tea …. ...
Pertussis toxin (PT) is a protein-based AB5-type exotoxin produced by the bacterium Bordetella pertussis, which causes whooping cough. PT is involved in the colonization of the respiratory tract and the establishment of infection. Research suggests PT may have a therapeutic role in treating a number of common human ailments, including hypertension, viral inhibition, and autoimmune inhibition. PT clearly plays a central role in the pathogenesis of pertussis although this was discovered only in the early 1980s. The appearance of pertussis is quite recent, compared with other epidemic infectious diseases. The earliest mention of pertussis, or whooping cough, is of an outbreak in Paris in 1414. This was published in Moultons The Mirror of Health, in 1640. Another epidemic of pertussis took place in Paris in 1578 and was described by a contemporary observer, Guillaume de Baillou. Pertussis was well known throughout Europe by the middle of the 18th century. Jules Bordet and Octave Gengou described in ...
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Banked acute-phase and convalescent-phase serum samples from a previous study of respiratory illness in university students were examined for significant (≥2-fold) increases in ELISA titers of IgA and IgG antibody to Bordetella pertussis filamentous hemagglutinin, pertactin, and fimbriae-2 and ≥4-fold titer increases to agglutinogens by agglutination. ELISA titers of antibody to pertussis toxin could not be determined because of technical problems. Chlamydia pneumoniae infections were diagnosed by culture or by a ≥4-fold increase in immunofluorescence assay titer or a single high titer (≥512). Mycoplasma pneumoniae, influenza A and B, adenovirus, and respiratory syncytial virus infections were diagnosed by ≥4-fold increases in complement fixation titer or a single high titer (≥64). There were 319 subjects with cough of ≥5 days duration, and of these, 47 (15%) had significant increases in antibody to B. pertussis antigens; 26 (8%) had significant increases to fimbriae-2 or ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Escaneado de imágenes de microscopio electrónico de Bordetella pertussis - Gram-negativa, aerobia, no móviles, cocobacilos procariotas (bacterias) que causa la tosferina o pertussis. Sobre la técnica de imagen: Las tecnologías modernas tales como la microscopía electrónica puede dar detalles más finos de las bacterias que la microscopía óptica (luz) e incluso puede ser utilizado para mostrar las características internas. Las micrografías electrónicas son imágenes en blanco y negro generados por los rayos de alta energía de electrones. Las micrografías a veces se les da un color falso para que sean visualmente atractivo (en este caso, se utilizó un tinte verde).. ...
False colour transmission electron micrograph of the whooping cough bacterium, Bordetella pertussis. The micrograph shows the bacteriums surface covered in fine hairs called pili or frimbriae. Several types of pili have been identified, based on shape & function. Generally, pili cause bacteria to stick together & attach to foreign cells in the body. The fragments around the bacterium are bits of the growth medium. The whooping cough bacteria parasitise only humans, causing a respiratory tract infection characterised by fits of coughing that end in loud inspiratory whoops. It is potentially fatal in infancy. Magnification: X 20,600 at 35mm size. Original is bw print b220/213. - Stock Image B220/0213
False colour transmission electron micrograph (TEM) of a thin section of the whooping cough bacteria, Bordetella pertussis. The whooping cough bacteria parasitise only humans. They cause a respiratory tract infection characterised by fits of coughing that end in loud inspiratory whoops. The infection is usually contracted in childhood and is potentially fatal in infancy. Adults are sometimes affected. The infection damages the epithelium lining the trachea and bronchi, impairing the beat of the cilia that keep the airways clean. Antibiotics are of very limited effect in treatment. Magnification: X 8800 at 35mm size. - Stock Image B220/0221
ICD-10 A37.00 is whooping cough due to bordetella pertussis without pneumonia (A3700). This code is grouped under diagnosis codes for certain infectious and parasitic diseases.
BD Difco™ Fluorescent Antibody Reagents FA Bordetella Pertussis; 5mL BD Difco™ Fluorescent Antibody Reagents Antibody Binding Proteins and...
Catalytic and Structural Insights into Toxin Activation by the Bordetella pertussis CyaC-Acyltransferase av Dr. Niramon Thamwiriyasati Burapha University (BUU), Faculty of Allied Health Sciences, Chonburi, Thailand.
There was a report out yesterday that the cases of whooping cough (Bordetella pertussis) in Minnesota have doubled from last month and by May, have exceeded all cases reported there last year. There have been 700 cases so far this … Continue reading →. ...
Biohazard level, growth media and temperature, gram stain, industrial applications and more information for Bordetella pertussis.
Pertussis, also known as whooping cough, is a highly contagious respiratory disease. It is caused by the bacterium Bordetella pertussis.. Pertussis is known for uncontrollable, violent coughing which often makes it hard to breathe. After fits of many coughs, someone with pertussis often needs to take deep breathes which result in a "whooping" sound. Pertussis most commonly affects infants and young children and can be fatal, especially in babies less than 1 year of age.. The best way to protect against pertussis is immunization. ...
Pertussis is a highly contagious infection of the respiratory tract. It is caused by the bacterium Bordetella pertussis ( a gram-negative coccobacillus). Whooping cough gets its name from the whooping sound the child makes when trying to draw a breath after a coughing spell. The incubation period is thought to be 7-10 days (range 4-21 days). Pertussis is transmitted from person to person via aerosolized droplets produced from a cough or sneeze or by direct contact with secretions from the respiratory tract of infectious individuals. Individuals with pertussis are most infectious (contagious) during the catarrhal period when the person has symptoms similar to a head cold, and the first two weeks after the onset of the cough (i.e. approximately 21 days total). Some individuals, such as infants who remain culture-positive for several weeks, may be infectious for a longer period.. Children who are too young to be fully vaccinated and those who have not completed the primary vaccination series are at ...
B. pertussis, the organism that causes pertussis, elaborates multiple toxins, including tracheal cytotoxin, which damages the respiratory epithelial tissue in vitro (24), and pertussis toxin, which has systemic effects (e.g., promoting lymphocytosis) (25). Illnesses caused by other species of Bordetella are not considered preventable by available pertussis vaccines (26,27). Clinical Features B. pertussis infections and reinfections among adults and adolescents can be asymptomatic or range from a mild cough illness to the severe, prolonged cough illness of classic pertussis (28). The clinical presentation of pertussis can be similar to that for respiratory illness caused by B. parapertussis, B. bronchiseptica, B. holmseii, Mycoplasma pneumoniae, Chlamydia (Chlamydophila) pneumoniae, and multiple viral agents (e.g., adenovirus, parainfluenza virus, human metapneumovirus, influenza virus, rhinovirus, and coronavirus). The incubation period for pertussis typically is 7--10 days (range: 5--21 days) ...
Pertussis (also known as whooping cough) is an upper respiratory tract infection (URTI) characterised by a severe cough. Bordetella pertussis is the typical aetiological agent. [1] Mattoo S, Cherry JD. Molecular pathogenesis, epidemiology, and clinical manifestations of respiratory infections due to Bordetella pertussis and other Bordetella subspecies. Clin Microbiol Rev. 2005;18:326-382. http://www.ncbi.nlm.nih.gov/pubmed/15831828?tool=bestpractice.com [2] Kretsinger K, Broder KR, Cortese MM, et al. Preventing tetanus, diphtheria, and pertussis among adults: use of tetanus toxoid, reduced diphtheria toxoid and acellular pertussis vaccine. MMWR Recomm Rep. 2006;55:1-33. http://www.cdc.gov/mmwr/preview/mmwrhtml/rr5517a1.htm http://www.ncbi.nlm.nih.gov/pubmed/17167397?tool=bestpractice.com [3] Cortese MM, Baughman AL, Brown K, et al. A new age in pertussis prevention new opportunities through adult vaccination. Am J Prev Med. 2007;32:177-185. ...
Pertussis, also known as whooping cough, is an acute respiratory infectious disease caused by a bacteria called Bordetella Pertussis. The characteristic symptoms are paroxysmal cough, inspiratory wheezing and post-tussive vomiting. Following the inhalation of respiratory secretions from an infected individual, bacteria enter the upper respiratory tract and adhere to epithelial cells. Several adhesion factors have been implicated: the filamentous hemagglutinin (FHA), fimbriae, and pertactin (Prn). Pertussis toxin (Ptx) and adenylate cyclase toxin (ACT) have been identified so far as major protein toxins of B. pertussis. PTX is a hexameric AB5-type exotoxin. Catalytic A subunit catalyzes the ADP-ribosylation of the Gi subunits of the heterotrimeric G protein, then inhibits multiple downstream pathways. ACT is able to penetrate the cytoplasmic membrane of host cells and becomes activated through the cleavage and the binding of calmodulin (CaM). Activated ACT converts ATP to cyclic AMP and subverts ...
Whooping cough or Pertussis is a respiratory tract infection that induces violent coughing. Its caused by the bacteria Bordetella pertussis and goes lungs.
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The determination of the genome sequences of B. pertussis, B. parapertussis, and B. bronchiseptica was completed and the sequences were reported in 2003 by Parkhill et al. (18). We utilized early (August 2001) assemblies of these genome sequences to search for PCR target sequences that might be specific to B. pertussis. Two regions with significant sequence divergence were identified and utilized for the design of the BP283 and BP485 real-time PCR assays. Notably, the diagnostic potential of the region used for the design of our BP485 assay was also predicted previously by others using microarray-based comparative genome hybridization (4). Similarly, previous work using representational difference analysis described the genome region encompassing the BP283 target sequence as being specific to B. pertussis (15). Both assays demonstrated excellent sensitivities and specificities when applied to clinical isolates and nasopharyngeal specimens. In contrast to the IS481 assay, the BP283 and BP485 ...
Bordetella pertussis toxin IgG for suspected cases over 12 months of age with cough ,14 days duration and who have not been immunised against pertussis in the past year. The IgG test can not be used to determine immunity as there are currently no agreed correlated of protection. Bordetella pertussis DNA for suspected cases with cough ,21 days duration and within 48 hours of antibiotic therapy.. ...
Pertussis is an acute respiratory infection caused by Bordetella pertussis. Rates of recent B. pertussis infection between 8%--26% have been reported among adults with cough illness of at least 5 days duration who sought medical care. The CDC recommends vaccinating patients aged 15 to 64 years old, once in 10 years. Although acellular vaccines such as BOOSTRIX have been evaluated in healthy population, the safety and efficacy of this vaccine in patients suffering from rheumatic diseases have not been established.. Study population : 50 Rheumatoid Arthritis (RA) patients and 5 healthy controls. Evaluation : the evaluation will be performed on week 0 and 4-6 weeks later. In terms of safety, the patients will be evaluated according to the Disease Activity Index (DAS). Blood will be drawn at each visit at tested for humoral response to tetanus and pertussis. ...
Pertussis is an acute respiratory infection caused by Bordetella pertussis. Rates of recent B. pertussis infection between 8%--26% have been reported among adults with cough illness of at least 5 days duration who sought medical care. The CDC recommends vaccinating patients aged 15 to 64 years old, once in 10 years. Although acellular vaccines such as BOOSTRIX have been evaluated in healthy population, the safety and efficacy of this vaccine in patients suffering from rheumatic diseases have not been established.. Study population : 50 Rheumatoid Arthritis (RA) patients and 5 healthy controls. Evaluation : the evaluation will be performed on week 0 and 4-6 weeks later. In terms of safety, the patients will be evaluated according to the Disease Activity Index (DAS). Blood will be drawn at each visit at tested for humoral response to tetanus and pertussis. ...
BackgroundThe objective of the study was to analyse the evolution of Bordetella pertussis population and the influence of herd immunity in different areas of the world where newborns and infants are highly vaccinated.MethodologyThe analysis was performed using DNA microarray on 15 isolates, PCR on 111 isolates as well as GS-FLX sequencing technology on 3 isolates and the B. pertussis reference strain, Tohama I.Principal FindingsOur analyses demonstrate that the current circulating isolates are continuing to lose genetic material as compared to isolates circulating during the pre-vaccine era whatever the area of the world considered. The lost genetic material does not seem to be important for virulence. Our study confirms that the use of whole cell vaccines has led to the control of isolates that were similar to vaccine strains. GS-FLX sequencing technology shows that current isolates did not acquire any additional material when compared with vaccine strains or with isolates of the pre-vaccine era and
La tos ferina es una enfermedad del tracto respiratorio que se presenta exclusivamente en los seres humanos y es causada por la bacteria gramnegativa Bordetella pertusis.
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Photo of Bordetella Pertussis, Photo of Pertussis in a newborn baby, Photo of Pertussis in a Child, Photo of Pertussis in an Adult, Photo of Thick Respiratory Secretions in a Pertussis Patient, X-Ray Photo of Pneumonia Complication due to Pertussis.
2 PULSED-FIELD GEL ELECTROPHORESIS (PFGE) (a) Basic principles A solution to the problems of analysis caused by complex chromosomal fingerprint patterns is to use a rare-cutting restriction endonuclease to generate only a limited number of large DNA fragments. As stated in the previous section, such large fragments cannot be separated readily by conventional agarose gel electrophoresis. The only technique available currently that is capable of separating DNA molecules in the 50 Analysis of chromosomal DNA 45 kb-12 Mb range physically is pulsed-field gel electrophoresis (PFGE), first described by Schwartz and Cantor (1984). 345-76. B. I. (1989) Serotyping Bordetella pertussis strains. Vaccine, 7, 491-4. , Polveroni, G. and Abadie, G. (1988) Serotyping of Pasteurella haemolytica - comparison and adjustment of antigenic extracts and techniques. Revue de Medecine Veterinaire, 139, 719-22. , Nicklon, S. R. (1977) DNA sequencing with chainterminating inhibitors. Proceedings of the National Academy ...
CBER, FDA. A postdoctoral fellow position is immediately available within the Laboratory of Respiratory Pathogens at the FDAs Center for Biologics Evaluation & Research in Silver Spring, Maryland. The candidate will have the opportunity to participate in a well-funded research program utilizing the baboon model of pertussis to advance our understanding of pertussis disease and immunology and will be expected to conduct independent research with a focus on pathogenesis and the host response to Bordetella pertussis infection. ...
What is pertussis?. Pertussis (also called "whooping cough") is a respiratory illness caused by bacteria that is easily spread from person to person. A person with pertussis can have severe coughing spasms that last for weeks. Is pertussis dangerous?. Pertussis is usually mild in older children and adults, but can be dangerous for infants and young children. Although rare, pertussis can cause serious health and breathing problems such as pneumonia, seizures, and swelling of the brain (encephalopathy), especially among infants less than six months of age. How is it spread?. The bacteria that causes pertussis lives in the nose, mouth and throat and is sprayed into the air when an infected person sneezes, cough or talks. People nearby can then breathe in the germs. Spread of pertussis occurs by droplets or direct contact with mucus or saliva from an infected person. People with pertussis can spread the disease starting two weeks before until three weeks after their cough starts. However, treatment ...
Background: Re-emergence of pertussis has been reported in Iran despite a high rate of vaccination coverage. Low efficacy of the vaccine might be due
Abcam provides general protocols for Human Anti-Bordetella pertussis IgA ELISA Kit (ab108708). Please download our pdf protocol booklet
11/2017 There have been some unexpected changes to the OpenWetWare platform, affecting the formatting of the Banta Lab Website. We are currently working on addressing these issues. A new website is under development and should be launched soon. 10/2017 The Banta Lab is pleased to welcome another new graduate student into the lab group. Nadim Massad has joined the research group this semester. 9/2017 A paper entitled "Block V RTX domain of adenylate cyclase from Bordetella pertussis: A conformationally dynamic scaffold for protein engineering applications" by Bulutoglu and Banta was published in Toxins as an Invited Review for the Special Issue: Adenylate Cyclase (CyaA) Toxin 9/2017 A paper entitled "Catch and release: Engineered allosterically-regulated β-roll peptides enable on/off biomolecular recognition" by Bulutoglu, Dooley, Szilvay, Blenner and Banta was published in ACS Synthetic Biology. 9/2017 A paper entitled "Block V RTX domain of adenylate cyclase from Bordetella pertussis: A ...
11/2017 There have been some unexpected changes to the OpenWetWare platform, affecting the formatting of the Banta Lab Website. We are currently working on addressing these issues. A new website is under development and should be launched soon. 10/2017 The Banta Lab is pleased to welcome another new graduate student into the lab group. Nadim Massad has joined the research group this semester. 9/2017 A paper entitled "Block V RTX domain of adenylate cyclase from Bordetella pertussis: A conformationally dynamic scaffold for protein engineering applications" by Bulutoglu and Banta was published in Toxins as an Invited Review for the Special Issue: Adenylate Cyclase (CyaA) Toxin 9/2017 A paper entitled "Catch and release: Engineered allosterically-regulated β-roll peptides enable on/off biomolecular recognition" by Bulutoglu, Dooley, Szilvay, Blenner and Banta was published in ACS Synthetic Biology. 9/2017 A paper entitled "Block V RTX domain of adenylate cyclase from Bordetella pertussis: A ...
B pertussis produces numerous virulence factors, including toxins and attachment agents, many of which are antigenic and included in the acellular vaccine. The link of each virulence factor to clinical illness has been difficult to elucidate due to lack of an animal model for experimentation. However, a recently developed model in infant baboons has the potential to address unanswered questions. The bacteria attach to ciliated epithelial cells of the respiratory tract, induce ciliary paralysis and local inflammation, and thicken and decrease clearance of secretions. B pertussis is not invasive. Pertussis toxin, necessary but not sufficient to cause clinical pertussis, is secreted by the bacteria and affects G-protein function, which prevents migration of lymphocytes to the area of infection, and inhibits the function of neutrophils, macrophages, monocytes, and lymphocytes. Adenylate cyclase toxin invades phagocytes and induces high levels of cyclic adenosine monophosphate (AMP), which impairs ...
Diagnosis Code A37.00 information, including descriptions, synonyms, code edits, diagnostic related groups, ICD-9 conversion and references to the diseases index.
Specimen Collection: Infectious material should be submitted directly to the laboratory without delay and protected from excessive heat and cold. If possible, it is recommended to inoculate the plate at the patients bedside. If there is to be a delay in processing, the specimen should be inoculated onto an appropriate transport medium and refrigerated until inoculation. Consult listed references for information on specimen collection. (1-5) Refer to the keywords "Specimen Collection and Transport" on the Hardy Diagnostics Technical Document website for more information.. Method of Use for Regan-Lowe Deep (Cat. no. Q32): The medium should be brought to room temperature prior to inoculation. Providing the physical nature of the specimen is suitable for a stab inoculation, submerge the specimen into the medium. If using a swab, the tip must be submerged well into the medium. Break or cut any portion of the swab that is protruding from the tube. Tighten the cap and deliver immediately to the ...
The CPT codes provided are based on AMA guidelines and are for informational purposes only. CPT coding is the sole responsibility of the billing party. Please direct any questions regarding coding to the payer being billed ...
Diagnosing pertussis in the early stages can be difficult, because early signs and symptoms are often nonspecific. Polymerase chain reaction (PCR) testing, a technique used to detect DNA sequences specific for Bordetella pertussis, is used for diagnosis; however, results should be cautiously interpreted, because they can often be false positive or false negative. The Centers for Disease Control and Prevention (CDC) has developed best practices to help optimize the use of PCR testing for the diagnosis of pertussis.
Pertactin is a virulence factor for B. pertussis which is an adaptation of the organism that enables it to colonise its host. Pertactin is an adhesin that promotes adhesion of B. pertussis bacteria to the tracheal epithelium of humans. Pertactin (Prn) is one of the antigens in the acellular vaccine along with inactivated pertussis toxin (PT), filamentous hemagglutinin (FHA) and fimbriae types 2 and 3 (Fim). The U.S. group, as reported in NEJM, found both insertions and stop codons that prevented the B. pertussis bacterium from expressing pertactin although the virulence of the pertactin negative strains was the same as pertactin positive strains ...
... , also commonly called whooping cough, is a bacterial infection of the respiratory tract caused by Bordetella pertussis.
ECDC is addressing the harmonisation and improvement of pertussis diagnosis for surveillance and outbreak detection/monitoring in order to assure quality and comparability of data. The guidance and protocol are intended for real-time PCR on DNA extracted from clinical specimens obtained from patients with suspected whooping cough (i.e. Bordetella pertussis or B. parapertussis). ...
ECDC is addressing the harmonisation and improvement of pertussis diagnosis for surveillance and outbreak detection/monitoring in order to assure quality and comparability of data. The guidance and protocol are intended for real-time PCR on DNA extracted from clinical specimens obtained from patients with suspected whooping cough (i.e. Bordetella pertussis or B. parapertussis). ...
(CIDRAP News) - Researchers in other countries have found evidence that circulating strains of Bordetella pertussis have adapted to the acellular vaccine, and researchers today reported similar findings for the first time in US kids, based on genetic analysis of isolates from hospitalized children.
Pertussis (also known as whooping cough) continues to be a global health problem with an estimated 45 million cases annually and 300,00 deaths, which occur most...
Whooping Cough- Clinical and epidemiological aspect of pertussis, including current trends in spread and surveillance in the United States. Details the major issues associated with vaccination in children, adolescents, and adults. Identify the clinical features and discuss medical management of Bordetella pertussis. Outline the epidemiologic features of pertussis, including occurrence, transmission, and communicability. Review current…
Although a vaccine has been developed against whooping cough, which is routinely given to children in the first year of life, cases of the disease still occur, especially in infants younger than 6 months of age.. According to the CDC, there has been a dramatic increase in the number of cases of pertussis since the 1980s, especially in preteens and teens 10 to19 years of age and in babies less than 5 months of age. The CDC recommends that children receive five DTaP shots for maximum protection against pertussis. A DTaP shot is a combination vaccine that protects against three diseases: diphtheria, tetanus, and pertussis. The first three shots are given at 2, 4, and 6 months of age. Between 15 and 18 months of age, the fourth shot is given, and a fifth shot when a child enters school at 4 to 6 years of age. At regular checkups for 11- or 12-year-olds, a preteen should get a dose of Tdap. The Tdap booster contains tetanus, diphtheria, and pertussis. If an adult did not get a Tdap as a preteen or ...
CIDRAP News) - Researchers in other countries have found evidence that circulating strains of Bordetella pertussis have adapted to the acellular vaccine, and researchers today reported similar findings for the first time in US kids, based on genetic analysis of isolates from hospitalized children. ...
Micro-landscape view of respiratory cells, accumulating mucus, and Bordetella pertussis bacteria releasing toxins. A coughing event is occurring here, displacing mucus, the bacteria, and debris. Also known as whooping cough, pertussis is a highly contagious airborne disease.
This evaluation identified an increase in incidence rates and risk ratios of reported pertussis in the 6 years after receipt of the fifth DTaP dose, strongly suggesting waning of vaccine-induced immunity. Despite considerably different rates of pertussis in Minnesota and Oregon during the period of investigation, similar trends of increase in risk ratios were observed in both states.. The level of protection immediately after completion of the childhood DTaP series is high, with postlicensure vaccine effectiveness estimates ranging from 88.7% to 97%.15,16 However, recent studies as well as our investigation demonstrate that protection from the DTaP series begins to wane after vaccination, contributing to the accumulation of vaccinated individuals who are still susceptible to disease.15,17-19 Assuming a constant attack rate of pertussis across age groups, this growing pool of susceptible persons helps to explain the emergence of an increased burden of disease among 7- to 10-year-olds, a group ...
Geduhn, Jens, Dove, Stefan, Shen, Yuequan, Tang, Wei-Jen, König, Burkhard und Seifert, Roland (2011) Bis-Halogen-Anthraniloyl-Substituted Nucleoside 5-triphosphates as Potent and Selective Inhibitors of Bordetella pertussis CyaA. The Journal of pharmacology and experimental therapeutics 336 (1), S. 104-115 ...
z angličtiny preložil Ing. Marián Fillo.. Správy o epidémiách čierneho kašľa v Kalifornii1, 2 a iných štátoch toto leto nie sú ničím novým. Každých 4-5 rokov - bez ohľadu na percento zaočkovanosti - je hlásený nárast čierneho kašľa.. Čierny kašeľ je respiračným ochorením (t.j. ochorením dýchacích ciest). Toxíny v baktérii Bordetella pertussis stimulujú produkciu veľkého množstva hustého lepkavého hlienu, ktorý môže upchať dýchacie cesty malých bábätiek a detí, čo im sťaží dýchanie a spôsobuje vracanie, škrtenie a vyludzovanie pískavého zvuku3, keď sa snažia dýchať.. Jestvuje nebunková (acelulárna) vakcína proti čiernemu kašľu - DTaP - ktorá bola schválená pre deti v USA v roku 1996.4 DTaP nahradila staršiu celobunkovú vakcínu proti čiernemu kašľu - DPT - ktorá často vyvolávala negatívne reakcie a bola spojená s viacerými prípadmi vysokej horúčky, kolapsu/šoku, kŕčov, zápalov mozgu a trvalého poškodenia ...
Si los resultados obtenidos permiten establecer de forma provisional el diagnóstico, ha de indicarse el tratamiento pertinente. En caso de no obtener el diagnóstico o bien cuando la tos persiste a pesar del tratamiento, el enfermo debe remitirse al especialista. I Las fases II y III deben reservarse, por su complejidad, a centros especializados y al ámbito hospitalario. Así, si la tos persiste a pesar del tratamiento han de realizarse pruebas más específicas del asma, como un test de broncoprovocación con metacolina o histamina, el estudio de un esputo inducido o el recuento de los eosinófilos del esputo. Los agentes más frecuentemente implicados son Mycoplasma spp. y Bordetella pertussis. La tos persiste hasta en la mitad de los pacientes que sufren la infección. Se recomiendan los broncodilatadores o los esteroides si la tos afecta a la calidad de vida del individuo. Exposición ocupacional Hay que investigar la exposición a los irritantes inhalados de bajo peso molecular, al ...
PERTUSSIS - Pertussis (whooping cough) may be mild or serious and is easily passed from person to person. Pertussis can cause spells of coughing and choking that make it hard to eat, drink or breathe. The coughing can last for weeks. Pertussis is most dangerous to babies under one year old. Babies with pertussis are so sick that nearly half must go to the hospital. About one baby in 100 with pertussis either dies or is left with permanent brain injury. Serious illness is less likely in older children and adults ...
February 4, 2009) Overall, Michigan saw a significant increase in reported cases of pertussis (Whooping Cough) in the second half of 2008 compared with the first half of the year. As of the end of December, a total of 307 cases had been reported in the state, although that number is subject to change as work continues to finalize case investigations. More than twice as many cases (210) were reported July through December compared to the first half of the year. Most cases of pertussis are preventable with immunization, said Joshua Meyerson, MD, Medical Director for the Health Department of Northwest Michigan. About six cases of pertussis are reported in the Health Department s four-county Health District each year, he said.. The single most important thing parents can do to protect their children from vaccine-preventable illnesses like pertussis is to get their infants vaccinated and follow the schedule for booster doses as their babies grow. We may not see very many cases of Whooping Cough but ...
In a nonoutbreak setting, data to determine the diagnostic usefulness of symptoms classically associated with pertussis are limited and of relatively weak quality. The presence or absence of posttussive emesis or inspiratory whoop modestly change the likelihood of pertussis; therefore, clinicians mu …
Nobody means to put their child at risk. Thats why its so devastating when a child becomes ill or dies from pertussis," Dolan said. "When the statistics were in a young child and when they were able to identify where the source came from, 80 percent of the time, it came from a family member.". ...
The Livingston County Health Center is encouraging local residents to take advantage of numerous opportunities to get vaccinated against tetanus, diphtheria and pertussis in July.
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Diagnozes apstiprinošas metodes izvēle ir atkarīga no slimības ilguma kopš sākušās klepus lēkmes, pacienta vecuma un laika pēc pēdējās vakcinācijas vai revakcinācijas. B. pertussis izolēšana no klīniskā parauga tiek uzskatīta par diagnostikas zelta standartu (pamatojoties uz metodes augsto specifiskumu). B. pertussis nukleīnskābju (DNS) noteikšanas metode ar polimerāzes ķēdes reakciju (PĶR). Šai metodei ir augsts specifiskums un augsta jutība. Abas minētās metodes ir neaizvietojamas gadījumos, kad seroloģiskie testi var būt nepārliecinoši un grūti interpretējami, - jaundzimušajiem, zīdaiņiem līdz 12 mēnešu vecumam un nesen vakcinētiem vai revakcinētiem bērniem, pusaudžiem vai pieaugušajiem (ja pēc vakcinācijas ir pagājuši mazāk nekā 12 mēneši). Abas metodes ir kvalitatīvas, un rezultāts interpretējams kā pozitīvs vai negatīvs. B. pertussis specifisko IgG-anti-PT noteikšana (IgG klases antivielas pret B. pertussis toksīnu). ...
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The Sanger Institute has been funded by the Wellcome Trust to sequence the genomes of Bordetella pertussis strain Tohama I, B. parapertussis strain 12822 and B. bronchiseptica strain RB50 in collaboration with Duncan Maskell and Andrew Preston of the Centre for Veterinary Science, Dept. of Clinical Veterinary medicine, The University of Cambridge. The sequences and analysis are described in: Parkhill et al (2003) Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica. Nature Genetics 35 32-40 (DOI: 10.1038/Ng1227), and have been submitted to EMBL/GenBank with the accession numbers: BX470248 (B. pertussis), BX470249 (B. parapertussis) and BX470250 (B. bronchiseptica). The three sequenced Bordetella strains have been deposited with the ATCC and NCTC under the following accession numbers: Bordetella parapertussis 12822: ATCC BAA-587, NCTC 13253 Bordetella bronchiseptica RB50: ATCC BAA-588, NCTC 13252 Bordetella pertussis Tohama ...
Gearing, A.J.; Bird, C.; Wadha, M.; Redhead, K., 1987: The primary and secondary cellular immune responses to whole cell Bordetella pertussis vaccine and its components
The effect of an extract of histamine-sensitizing factor (HSF) of Bordetella pertussis on the immune response of different strains of mice to ovalbumin (OA) was investigated with regard to optimal dose of antigen and adjuvant. It was observed that all strains of mice treated with HSF during immunization with OA demonstrated enhanced production of hemagglutinating antibodies, as compared to animals treated with antigen alone. This enhancement was generally not as great as that demonstrated when Al(OH)3 was the adjuvant. HSF also stimulated a reaginic antibody response (IgE) to OA, but not in all strains of mice. In reagin responders optimal responses were observed with high doses of both antigen and adjuvant, whereas low doses of both produced little or no response. Maximal reagin production occurred usually 14-28 days after immunization and persisted for long periods of time. An anamnestic reagin response was elicited upon secondary immunization with antigen alone, not only in mice immunized ...
Looking for online definition of Bordet-Gengou phenomenon in the Medical Dictionary? Bordet-Gengou phenomenon explanation free. What is Bordet-Gengou phenomenon? Meaning of Bordet-Gengou phenomenon medical term. What does Bordet-Gengou phenomenon mean?
Adenylate cyclase toxin (CyaA) from Bordetella pertussis can subvert host immune responses allowing bacterial colonization. Here we have examined its adjuvant and immunomodulatory properties and the possible contribution of lipopolysaccharide (LPS), known to be present in purified CyaA preparations. CyaA enhanced antigen-specific interleukin-5 (IL-5) and IL-10 production and immunoglobulin G1 antibodies to coadministered antigen in vivo. Antigen-specific CD4+-T-cell clones generated from mice immunized with antigen and CyaA had cytokine profiles characteristic of Th2 or type 1 regulatory T (Tr1) cells. Since innate immune cells direct the induction of T-cell subtypes, we examined the influence of CyaA on activation of dendritic cells (DC) and macrophages. CyaA significantly augmented LPS-induced IL-6 and IL-10 and inhibited LPS-driven tumor necrosis factor alpha and IL-12p70 production from bone marrow-derived DC and macrophages. CyaA also enhanced cell surface expression of CD80, CD86, and ...
Pertussis Vaccine: A suspension of killed Bordetella pertussis organisms, used for immunization against pertussis (WHOOPING COUGH). It is generally used in a mixture with diphtheria and tetanus toxoids (DTP). There is an acellular pertussis vaccine prepared from the purified antigenic components of Bordetella pertussis, which causes fewer adverse reactions than whole-cell vaccine and, like the whole-cell vaccine, is generally used in a mixture with diphtheria and tetanus toxoids. (From Dorland, 28th ed)
Since their introduction in the 1940s and 1950s, pertussis vaccines (mostly in combination with diphtheria and tetanus toxoids as diphtheria, tetanus, and pertussis vaccines) have been very efficient in reducing pertussis mortality and morbidity in infants and young children. WHO estimates suggest that between 1999 and 2014, more than 100 000 infant deaths could have been averted mainly by increased coverage of pertussis vaccination.1 Pertussis vaccines come in two varieties: one is made of whole-cell killed Bordetella pertussis cells, consequently called whole-cell pertussis vaccine, and the other is made from one to five purified and partly chemically inactivated bacterial virulence factors, consequently called acellular pertussis vaccine. ...
The adenylate cyclase toxin (AC toxin) is necessary for disease caused by Bordetella pertussis, which has reemerged in the United States over the last two decad...
Despite an increased proportion of Bordetella pertussis isolates lacking pertactin, vaccine effectiveness (VE) is still high in Vermont for the five-dose diphtheria, tetanus, and acellular pertussis vaccine (DTaP) series and the tetanus, diphtheria, and acellular pertussis vaccine (Tdap).
Reassessment of the role of whole-cell pertussis vaccine as a cause of permanent neurologic damage is necessitated by the 10-year follow-up of the National Childhood Encephalopathy Study (NCES) in Great Britain. The findings of this study demonstrate that infants and young children with serious acute neurologic disorders are at an increased risk of later neurologic impairment or death, irrespective of the initial precipitating event. The results, however, do not establish a causal relationship between pertussis vaccination and chronic neurologic abnormalities. The Academy reaffirms its earlier conclusion that whole-cell pertussis vaccine has not been proven to be a cause of brain damage and continues to recommend pertussis vaccination in accordance with the guidelines in the 1994 Red Book.. ...
Using antisera raised against serotype 2 and 3 fimbrial subunits from Bordetella pertussis, serologically related polypeptides were detected in Bordetella bronchiseptica, Bordetella parapertussis and Bordetella avium strains. The two B. pertussis fimbrial subunits, and three of the serologically related B. bronchiseptica polypeptides, were shown to be very similar in amino ... read more acid composition and N-terminal amino acid sequence. Homology was observed between the N-termini of these polypeptides, and fimbrial subunits from Escherichia coli, Haemophilus influenzae and Proteus mirabilis. A synthetic oligonucleotide probe, derived from the N-terminal sequence of the B. pertussis serotype 2 fimbrial subunit, was used to identify fimbrial genes in genomic Southern blots. The results suggested the presence of multiple fimbrial subunit genes in B. pertussis, B. bronchiseptica and B. parapertussis. The DNA probe was used to clone one of the three tentative fimbrial subunit genes detected in B. ...
Swelling involving the entire thigh or upper arm has been reported after booster doses of different acellular pertussis vaccines. Swelling of the entire thigh was reported among recipients of a booster dose of JNIH-6 (a two-component acellular pertussis vaccine produced by Biken [Japan] and comparable to the acellular pertussis component contained in Tripedia). During a study performed in Sweden during the 1980s, children who had previously received two or three doses of Biken acellular pertussis vaccine at age 6--8 months received a booster dose deep subcutaneously of the same vaccine at age 2 years. Certain children experienced substantial local reactions, including swelling of the entire thigh (16), although administration of vaccine subcutaneously could have influenced reaction rates in that study. Occurrence of extensive swelling involving the entire thigh of vaccinated children was reported among DTaP recipients in an open-label safety study in Germany during April 1993--November 1994, in ...
Bordetella is a genus of small (0.2 - 0.7 µm), Gram-negative coccobacilli of the phylum Proteobacteria. Bordetella species, with the exception of B. petrii, are obligate aerobes, as well as highly fastidious, or difficult to culture. All species can infect humans. The first three species to be described (B. pertussis, B. parapertussis, B. bronchiseptica,); are sometimes referred to as the classical species. One of these (B. bronchiseptica) is also motile. B. pertussis and occasionally B. parapertussis cause pertussis or whooping cough in humans, and some B. parapertussis strains can colonise sheep. B. bronchiseptica rarely infects healthy humans, though disease in immunocompromised patients has been reported. B. bronchiseptica causes several diseases in other mammals, including kennel cough and atrophic rhinitis in dogs and pigs, respectively. Other members of the genus cause similar diseases in other mammals, and in birds (B. hinzii, B. avium). The Bordetella genus is named after Jules ...
Tetanus, diphtheria, and pertussis are serious diseases caused by bacteria.. Tetanus (lockjaw) causes painful tightening of the muscles, usually all over the body. It can lead to "locking" of the jaw so the victim cannot open the mouth or swallow. Tetanus leads to death in about 1 out of 10 cases.. Diphtheria causes a thick coating in the nose, throat, and airways. It can lead to breathing problems, paralysis, heart failure, or death.. Pertussis (whooping cough) causes coughing so severe that it interferes with eating, drinking, or breathing. These spells can last for weeks and can lead to pneumonia, seizures (convulsions), brain damage, and death.. Diphtheria and pertussis are spread from person to person. Tetanus enters the body through a cut or wound.. The diphtheria, tetanus acellular, and pertussis adult vaccine (also called Tdap) is used to help prevent these diseases in people who are at least 10 years old. Most people in this age group require only one Tdap shot for protection against ...
Respiratory infections are common causes of morbidity and mortality. Chlamydia pneumoniae, Mycoplasma pneumoniae and Bordetella pertussis cause respiratory infection, often with similar symptoms. Molecular diagnostic methods are preferred since these bacteria are difficult to culture. The aim of this thesis was to evaluate and improve the diagnostics and knowledge of the epidemiology of these bacteria.. A real-time polymerase chain reaction (PCR) method targeting the IS481 element present in the genome of B. pertussis was compared to culture and serology results, and a duplex real-time PCR method was constructed for detecting C. pneumoniae and M. pneumoniae, which was compared to two endpoint PCR methods. Both real-time PCR methods showed high sensitivity and specificity.. Typing of 624 M. pneumoniae samples, collected from 1996 to 2017 from four counties, was performed by P1 typing and multiple-locus variable number tandem repeat analysis (MLVA). A polyclonal distribution of strains was seen ...
Achoo!. Feel like a sneeze or cough coming on? Cover it in a cloth or tissue paper, or even your sleeves, and wash your hands, admonishes the Centers for Disease Control and Prevention (CDC) - and for good reasons, too! Microbial pathogenic agents of a variety of respiratory illnesses, both viral [ranging from the common cold (rhinovirus); influenza (orthomyxovirus); parainfluenza viruses, respiratory syncytial virus (RSV), and human metapneumovirus (all paramyxoviruses); severe acute respiratory syndrome (SARS-Coronavirus)] as well as bacterial [such as those responsible for pneumonia (Streptococcus pneumoniae), whooping cough (Bordetella pertussis), and tuberculosis (Mycobacterium tuberculosis)] are often transmitted by cough, sneeze, and/or unclean hands/palms carrying these germs on their surfaces.. Continue reading. ...
With the pertussis outbreak in California nearing a 60-year high in the number of cases reported, the CDCs Advisory Committee on Immunization Practices, or ACIP, has voted to recommend the off-label use of tetanus, diphtheria and acellular pertussis, or Tdap, vaccine in two specific patient groups.
Bordetella pertussis/Bordetella parapertussis *Pertussis. γ. Enterobacteriales. (OX−). Lac+. *Klebsiella pneumoniae * ...
Bordetella pertussis, pneumococci, diphtheria, and rubella.[10] ...
위험 요인으로는 흡연, 먼지 및 기타 대기 오염에 대한 노출이 포함된다.[1] 폐렴미코플라스마(Mycoplasma pneumoniae) 나 백일해균(Bordetella pertussis)과 같은 박테리아나 고농도의 대기 오염에 ... Bordetella pertussis)과 같은 박테리아나 고농도의 대기 오염에 의한 경우도 소량 존재한다.[2][3]급성 기관지염의 치료에는 일반적으로 휴식, paracetamol (acetaminophen) 및 NSAIDs가 ...
čierny kašeľ - Bordetella pertussis. *tuberkulóza (TBC) - Mycobacterium tuberculosis. *záškrt - Corynebacterium diphtheriae. * ...
Corynebacterium diphtheriae and Bordetella pertussis.. *ubiquitination and SUMOylation. Various full-length, folded proteins ...
The 'a' in DTaP and Tdap stands for 'acellular,' meaning that the pertussis component contains only a part of the pertussis ... such as Bordetella bronchiseptica, has likely increased in recent years.[104] In some cases, most notably rabies, the parallel ... toxoids and pertussis (P) vaccine. Lower-case "d" and "p" denote reduced doses of diphtheria and pertussis used in the ... In the preparation for the 1990 Persian Gulf campaign, whole cell pertussis vaccine was used as an adjuvant for anthrax vaccine ...
Pertussis toxin (PTx) of Bordetella pertussis, formerly known as lymphocytosis-promoting factor, causes a decrease in the entry ...
Bordetella pertussis/Bordetella parapertussis *Pertussis. γ. Enterobacteriales. (OX−). Lac+. *Klebsiella pneumoniae * ...
Bordetella pertussis/Bordetella parapertussis *Pertussis. γ. Enterobacteriales. (OX−). Lac+. *Klebsiella pneumoniae * ...
Bordetella pertussis. *Legionella pneumophila. *Pasteurella multocida. Anaerobic Gram-positive bacteria *Clostridium ...
... and Bordetella pertussis may help prevent it. ...
Bordetella pertussis/Bordetella parapertussis *Pertussis. γ. Enterobacteriales. (OX−). Lac+. *Klebsiella pneumoniae * ...
... as well as Bordetella pertussis which causes whooping cough. Other members of the class can infect plants, such as Burkholderia ...
Diseases such as pertussis (or whooping cough) are cause by the bacteria Bordetella pertussis. This bacteria is marked by a ... The pertussis toxin is a protein exotoxin that binds to cell receptors by two dimers and reacts with different cell types such ... PCR is very efficient for diagnosing pertussis when compared to culture.[34] ... PCR is an important testing tool that can detect the sequences that are within the pertussis toxin gene. This is because PCR ...
Bordetella pertussis/Bordetella parapertussis *Pertussis. γ. Enterobacteriales. (OX-). Lac+. *Klebsiella pneumoniae * ...
Bordetella pertussis/Bordetella parapertussis *Pertussis. γ. Enterobacteriales. (OX−). Lac+. *Klebsiella pneumoniae * ...
... small number of cases are due to high levels of air pollution or bacteria such as Mycoplasma pneumoniae or Bordetella pertussis ... small number of cases are due to high levels of air pollution or bacteria such as Mycoplasma pneumoniae or Bordetella pertussis ... An exception is when acute bronchitis is due to pertussis. Tentative evidence supports honey and pelargonium to help with ...
The pertussis toxin (also an AB5 protein) produced by Bordetella pertussis acts in a similar manner with the exception that it ...
Bordetella pertussis/Bordetella parapertussis *Pertussis. γ. Enterobacteriales. (OX−). Lac+. *Klebsiella pneumoniae * ...
Bordetella pertussis. *Bradyrhizobium japonicum. *Burkholderia gladioli. C. *Campylobacter. *Campylobacterales. *Chlamydia ( ...
Pertussis is caused by the bacterium Bordetella pertussis.[4] It is an airborne disease which spreads easily through the coughs ... Pertussis is caused by the bacterium Bordetella pertussis. It is an airborne disease (through droplets) that spreads easily ... and Clinical Manifestations of Respiratory Infections Due to Bordetella pertussis and Other Bordetella Subspecies". Clin ... Ebell, MH; Marchello, C; Callahan, M (2017). "Clinical Diagnosis of Bordetella Pertussis Infection: A Systematic Review". J Am ...
Bordetella pertussis, the causative agent of whooping cough, secretes the pertussis toxin partly through the type IV system. ...
Bordetella pertussis/Bordetella parapertussis *Pertussis. γ. Enterobacteriales. (OX−). Lac+. *Klebsiella pneumoniae * ...
Pertussis (Whooping cough) Bordetella pertussis Plague Yersinia pestis Pneumococcal infection Streptococcus pneumoniae ...
gadā (Bordetella pertussis jeb Bordē-Žangū baktērija).[9] Slimības ierosinātājs ir Bordetella pertussis, nekustīga gramnegatīva ... Infant Pertussis Study Group. Transmission of Bordetella pertussis to young infants. Pediatr Infect Dis J., 2007 Apr;26(4):293- ... 21,0 21,1 21,2 ECDC Guidance and protocol for the serological diagnosis of human infection with Bordetella pertussis. 2012 Oct; ... Polymorphism of Bordetella pertussis Isolates Circulating for the Last 10 Years in France, Where a Single Effective Whole-Cell ...
Bordetella pertussis. Whooping cough. DPT vaccine. Boostrix, Adacel, Daptacel, Infanrix, Tripedia, Kinrix, Pediarix, Pentacel, ...
Bordetella pertussis/Bordetella parapertussis *Pertussis. γ. Enterobacteriales. (OX−). Lac+. *Klebsiella pneumoniae * ...
Burkholderia pseudomallei (Melioidosis) · Burkholderia mallei (Glanders) · Burkholderia cepacia complex · Bordetella pertussis/ ...
Genomic DNA from Bordetella pertussis Strain Tohama 1 TypeStrain=False Application: ... Bordetella pertussis (Bergey et al.) Moreno-Lopez (ATCC® BAA-589D-5™) Strain Designations: Genomic DNA from Bordetella ... Bordetella pertussis (Bergey et al.) Moreno-Lopez ATCC® BAA-589D-5™ dried At least 5 µg in 1X TE buffer. OD260/OD280: 1.6 to ... Quantitative Genomic DNA from Bordetella pertussis (ATCC® BAA-589DQ™) Add to frozen Specification range: ≥ 1 x 105 copies/µL. ...
Bordetella pertussis is the causative agent of pertussis. Here, we report the genome sequence of Bordetella pertussis strain CS ... Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica. ... Complete Genome Sequence of Bordetella pertussis CS, a Chinese Pertussis Vaccine Strain Shumin Zhang, Yinghua Xu, Zhemin Zhou, ... Bordetella pertussis is a strict human pathogen that is the causative agent of pertussis (whooping cough). Despite widespread ...
The Effect of Bordetella Pertussis on the Antibody Response in Mice to Type III Pneumococcal Polysaccharide. Ah Swee Kong and ... The Effect of Bordetella Pertussis on the Antibody Response in Mice to Type III Pneumococcal Polysaccharide ... The effect of an i.p. injection of Bordetella pertussis on the primary humoral immune response in mice to the thymus- ... The Effect of Bordetella Pertussis on the Antibody Response in Mice to Type III Pneumococcal Polysaccharide ...
The purified lymphocytosis promoting factor (LPF) from Bordetella pertussis was found to be a potent mitogen for peripheral ... The mitogenic effect of the lymphocytosis promoting factor from Bordetella pertussis on human lymphocytes. ... The mitogenic effect of the lymphocytosis promoting factor from Bordetella pertussis on human lymphocytes. Journal of Clinical ... Only one adult human serum, from a physician constantly working with B. pertussis, inhibited the mitogenic response to LPF and ...
Influence of bordetella pertussis on the lymphatic tissue of mice part 8 the influence of bordetella pertussis on the primary ... Barry, E.M.; Levine, M.M., 1998: Immune responses to the Bordetella pertussis antigens pertactin and pertussis toxin expressed ... to Bordetella pertussis antigens in children with a history of pertussis infection and in recipients of an acellular pertussis ... and agglutinogens 2 and 3 after infection with Bordetella pertussis and immunization with whole-cell pertussis vaccine. Class ...
... pertussis strains, and represent 84.8% of all CDSs found in the 171 B. pertussis strains. A total of 64 regions of difference ... pertussis isolates from different countries. The CGH microarray analysis estimated the core genome of B. pertussis, to consist ... pertussis. Our results show that B. pertussis is a dynamic organism that continues to evolve. ... In an attempt to gain insight into the genomic make-up of B. pertussis over the last 60 years, we used an oligonucleotide DNA ...
... including the three human pathogens Bordetella pertussis, Bordetella parapertussis an... ... The genus Bordetella consists of Gram-negative β-proteobacteria, ... Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica. ... including the three human pathogens Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica, which are ...
Ross, P.J., Lavelle, E.C., Mills, K.H.G. and A.P. Boyd `Adenylate cyclase toxin from Bordetella pertussis synergises with ... Adenylate cyclase toxin from Bordetella pertussis synergises with lipopolysaccharide to promote innate IL-10 production and ... Adenylate cyclase toxin (CyaA) from Bordetella pertussis can subvert host immune responses allowing bacterial colonization. ... Adenylate Cyclase Toxin from Bordetella pertussis Synergizes with Lipopolysaccharide To Promote Innate Interleukin-10 ...
Fatal Bordetella pertussis infection: report of two cases with novel pathologic findings. R Hackman, D G Perrin, M Karmali, E ... Pathology and pathogenesis of fatal Bordetella pertussis infection in infants. Christopher D Paddock, Gary N Sanden, James D ... Integrin-mediated localization of Bordetella pertussis within macrophages: role in pulmonary colonization. K Saukkonen, C ... BACKGROUND: Each year, Bordetella pertussis infection causes an estimated 294,000 deaths worldwide, primarily among young, ...
Imprinting levels on CD4+ T cells were compared to Bordetella parapertussis, a related strain that lacks pertussis toxin. Our ... One of the hallmarks of infection is lymphocytosis, which is induced by the pertussis toxin. Lymphocytes such as CD4+ T cells ... However, 25 days post-infection with B. pertussis, dendritic cells continued to express elevated levels of MHC class II, and ... We hypothesized that the pertussis toxin affects the imprinting process resulting in altered expression of trafficking ...
Bordetella pertussis adenylate cyclase. Penetration into host cells.: Exposure of Chinese hamster ovary, mouse adrenal cortex ... THP-1 and U-937 cells and human erythrocytes to adenylate-cyclase-containing urea extracts of Bordetella pertussis (strain 114 ... Anti-(B. pertussis) antibodies inhibit cyclase activity and prevent further cAMP accumulation after 10 min in cells previously ... pertussis organisms. We conclude that entry of the cyclase into cells is not by receptor-mediated endocytosis. ...
Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica. B. pertussis, and occasionally B. parapertussis, ... Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica.. ... Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica. ... NCTC 13253 Bordetella bronchiseptica RB50: ATCC BAA-588, NCTC 13252 Bordetella pertussis Tohama I: ATCC BAA-589, NCTC 13251 ...
9.1 Bordetella Pertussis Industry News. 9.2 Bordetella Pertussis Industry Development Challenges. 9.3 Bordetella Pertussis ... 1.1 Brief Introduction of Bordetella Pertussis. 1.2 Development of Bordetella Pertussis Industry. 1.3 Status of Bordetella ... 8.3 Effects to Bordetella Pertussis Industry. Chapter Nine Market Dynamics of Bordetella Pertussis Industry. ... 4.5 2011-2016 Chinese Import and Export of Bordetella Pertussis. Chapter Five Market Status of Bordetella Pertussis Industry. ...
... Academic Article ... The effect of an extract of histamine-sensitizing factor (HSF) of Bordetella pertussis on the immune response of different ...
The effect of exogenously added adenylate cyclase from Bordetella pertussis (strain 114) has been investigated in Y-1 mouse ... The effect of exogenously added adenylate cyclase from Bordetella pertussis (strain 114) has been investigated in Y-1 mouse ... Extracellular cAMP formation from host cell ATP by Bordetella Pertussis adenylate cyclase. ... Extracellular cAMP formation from host cell ATP by Bordetella Pertussis adenylate cyclase ...
Serum IgG titres against pertussis toxin (PT) are routinely used as a marker of recent or persisting B. pertussis infection. ... for pertussis amongst children with chronic coughs when used as a surrogate for the serum ELISA (assuming disease prevalence of ... Bordetella pertussis infection is being increasingly recognized as a cause of prolonged, distressing cough (without whooping ... 12-37%). This oral fluid ELISA will greatly assist in the convenience of B. pertussis disease diagnosis and surveillance. © ...
There is an acellular pertussis vaccine prepared from the purified antigenic components of Bordetella pertussis, which causes ... A suspension of killed Bordetella pertussis organisms, used for immunization against pertussis (WHOOPING COUGH). It is ... Pertussis Vaccine. Subscribe to New Research on Pertussis Vaccine A suspension of killed Bordetella pertussis organisms, used ... 08/01/1998 - "The efficacy of a whole cell pertussis vaccine and fimbriae against Bordetella pertussis and Bordetella ...
... the US replaced whole cell pertussis vaccines with acellular pertussis vaccines over safety concerns. A research letter to ... of the American Medical Association reports that the switch might be responsible for a recent rising number of pertussis cases ... "possible explanations for our findings could include antigenic shifts in circulating Bordetella pertussisstrains or the ... In the 1990s, the US replaced whole cell pertussis vaccines with acellular pertussis vaccines over safety concerns. A research ...
Bordetella parapertussis, , , ... World Heritage Encyclopedia, the aggregation of the largest online encyclopedias available, ... "The O Antigen Enables Bordetella parapertussis To Avoid Bordetella pertussis-Induced Immunity". Infection and Immunity 75 (10 ... "Clinical characteristics of illness caused by Bordetella parapertussis compared with illness caused by Bordetella pertussis". ... Bordetella parapertussis is a small Gram-negative bacterium of the genus Bordetella that is adapted to colonise the mammalian ...
Ca2 Influx and Tyrosine Kinases Trigger Bordetella Adenylate Cyclase Toxin ACT Endocytosis. Cell Physiology and Expression of ... Humans infected with Bordetella pertussis, the whooping cough bacterium, show evidences of impaired host defenses. This ... Ca2 Influx and Tyrosine Kinases Trigger Bordetella Adenylate Cyclase Toxin ACT Endocytosis. Cell Physiology and Expression of ... Ca2 Influx and Tyrosine Kinases Trigger Bordetella Adenylate Cyclase Toxin ACT Endocytosis. Cell Physiology and Expression of ...
... acellular pertussis vaccines (diphtheria-tetanus-acellular pertussis; DTaP) replaced whole cell vaccin ... Newly emerging clones of Bordetella pertussis carrying prn2 and ptxP3 alleles implicated in Australian pertussis epidemic in ... Newly emerging clones of Bordetella pertussis carrying prn2 and ptxP3 alleles implicated in Australian pertussis epidemic in ... Possible explanations for our findings could include antigenic shifts in circulating Bordetella pertussis strains2 or the ...
Bordetella pertussis / Streptococcus / Listeria Monocytogenes / Yersinia enterocolitica / Mycobacterium smegmatis / ...
This disease is similar to whooping cough caused by the related pathogen of humans, B. pertussis. Though the mortality rate for ... In a related manner, Bordetella avium is a Gram negative species of bacteria that causes bordetellosis in poultry. ... This disease is similar to whooping cough caused by the related pathogen of humans, B. pertussis. Though the mortality rate for ... In a related manner, Bordetella avium is a Gram negative species of bacteria that causes bordetellosis in poultry. ...
... vaccination.1 Pertussis vaccines come in two varieties: one is made of whole-cell killed Bordetella pertussis cells, ... Comment] Acellular pertussis vaccines: where to go to?. Since their introduction in the 1940s and 1950s, pertussis vaccines ( ... Results of our trial led to the licensure of new acellular pertussis vaccines containing genetically inactivated pertussis ... The new TdaP(PTgen/FHA) vaccine is safe and induces higher pertussis responses 28 days after vaccination than does the ...
A37.11 ICD 10 CM Code for Whooping cough due to Bordetella parapertussis with pneumonia, Convert ICD 10 CM code A37.11 to ICD 9 ... Whooping cough due to Bordetella pertussis. A37.1. Whooping cough due to Bordetella parapertussis ...
  • The cellular immune responses of Balb/c mice and Wistar rats immunized in hind footpads with intact killed Bordetella pertussis were found to differ from those of similar animals immunized with other bacteria including Bordetella bronchiseptica, Salmonella typhimurium and Escherichia coli. (eurekamag.com)
  • Exposure of Chinese hamster ovary, mouse adrenal cortex tumor (Y-1), THP-1 and U-937 cells and human erythrocytes to adenylate-cyclase-containing urea extracts of Bordetella pertussis (strain 114) organisms promotes the formation of large concentrations of intracellular cAMP. (mysciencework.com)
  • The effect of exogenously added adenylate cyclase from Bordetella pertussis (strain 114) has been investigated in Y-1 mouse adrenal tumor, chinese hamster ovary (CHO) and several other cells. (unimol.it)
  • Anti-(B. pertussis) antibodies inhibit cyclase activity and prevent further cAMP accumulation after 10 min in cells previously exposed to urea extract. (mysciencework.com)
  • From 1975 to 1985 a lower vaccine dose was used, which most likely lead to an increase in the incidence of pertussis between 1985 to 1987 [ 21 ]. (biomedcentral.com)
  • Compared with whole cell vaccines, acellular vaccines resulted in approximately a 600% increase in incidence rate of pertussis looking at reporting rates in Australian children between 1999 and 2011. (thedoctorschannel.com)
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