Bone Matrix: Extracellular substance of bone tissue consisting of COLLAGEN fibers, ground substance, and inorganic crystalline minerals and salts.Bone and Bones: A specialized CONNECTIVE TISSUE that is the main constituent of the SKELETON. The principle cellular component of bone is comprised of OSTEOBLASTS; OSTEOCYTES; and OSTEOCLASTS, while FIBRILLAR COLLAGENS and hydroxyapatite crystals form the BONE MATRIX.Bone Demineralization Technique: Removal of mineral constituents or salts from bone or bone tissue. Demineralization is used as a method of studying bone strength and bone chemistry.Bone Remodeling: The continuous turnover of BONE MATRIX and mineral that involves first an increase in BONE RESORPTION (osteoclastic activity) and later, reactive BONE FORMATION (osteoblastic activity). The process of bone remodeling takes place in the adult skeleton at discrete foci. The process ensures the mechanical integrity of the skeleton throughout life and plays an important role in calcium HOMEOSTASIS. An imbalance in the regulation of bone remodeling's two contrasting events, bone resorption and bone formation, results in many of the metabolic bone diseases, such as OSTEOPOROSIS.Bone Resorption: Bone loss due to osteoclastic activity.Extracellular Matrix: A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere.Bone Density: The amount of mineral per square centimeter of BONE. This is the definition used in clinical practice. Actual bone density would be expressed in grams per milliliter. It is most frequently measured by X-RAY ABSORPTIOMETRY or TOMOGRAPHY, X RAY COMPUTED. Bone density is an important predictor for OSTEOPOROSIS.Bone Development: The growth and development of bones from fetus to adult. It includes two principal mechanisms of bone growth: growth in length of long bones at the epiphyseal cartilages and growth in thickness by depositing new bone (OSTEOGENESIS) with the actions of OSTEOBLASTS and OSTEOCLASTS.Osteogenesis: The process of bone formation. Histogenesis of bone including ossification.Bone Regeneration: Renewal or repair of lost bone tissue. It excludes BONY CALLUS formed after BONE FRACTURES but not yet replaced by hard bone.Calcification, Physiologic: Process by which organic tissue becomes hardened by the physiologic deposit of calcium salts.Osteoblasts: Bone-forming cells which secrete an EXTRACELLULAR MATRIX. HYDROXYAPATITE crystals are then deposited into the matrix to form bone.Bone Transplantation: The grafting of bone from a donor site to a recipient site.Bone Neoplasms: Tumors or cancer located in bone tissue or specific BONES.Bone Substitutes: Synthetic or natural materials for the replacement of bones or bone tissue. They include hard tissue replacement polymers, natural coral, hydroxyapatite, beta-tricalcium phosphate, and various other biomaterials. The bone substitutes as inert materials can be incorporated into surrounding tissue or gradually replaced by original tissue.Osteocytes: Mature osteoblasts that have become embedded in the BONE MATRIX. They occupy a small cavity, called lacuna, in the matrix and are connected to adjacent osteocytes via protoplasmic projections called canaliculi.Bone Marrow: The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.Osteocalcin: Vitamin K-dependent calcium-binding protein synthesized by OSTEOBLASTS and found primarily in BONES. Serum osteocalcin measurements provide a noninvasive specific marker of bone metabolism. The protein contains three residues of the amino acid gamma-carboxyglutamic acid (Gla), which, in the presence of CALCIUM, promotes binding to HYDROXYAPATITE and subsequent accumulation in BONE MATRIX.Extracellular Matrix Proteins: Macromolecular organic compounds that contain carbon, hydrogen, oxygen, nitrogen, and usually, sulfur. These macromolecules (proteins) form an intricate meshwork in which cells are embedded to construct tissues. Variations in the relative types of macromolecules and their organization determine the type of extracellular matrix, each adapted to the functional requirements of the tissue. The two main classes of macromolecules that form the extracellular matrix are: glycosaminoglycans, usually linked to proteins (proteoglycans), and fibrous proteins (e.g., COLLAGEN; ELASTIN; FIBRONECTINS; and LAMININ).Bone Marrow Cells: Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.Integrin-Binding Sialoprotein: A highly glycosylated and sulfated phosphoprotein that is found almost exclusively in mineralized connective tissues. It is an extracellular matrix protein that binds to hydroxyapatite through polyglutamic acid sequences and mediates cell attachment through an RGD sequence.Osteoclasts: A large multinuclear cell associated with the BONE RESORPTION. An odontoclast, also called cementoclast, is cytomorphologically the same as an osteoclast and is involved in CEMENTUM resorption.Osteopontin: A negatively-charged extracellular matrix protein that plays a role in the regulation of BONE metabolism and a variety of other biological functions. Cell signaling by osteopontin may occur through a cell adhesion sequence that recognizes INTEGRIN ALPHA-V BETA-3.Bone Diseases: Diseases of BONES.Sialoglycoproteins: Glycoproteins which contain sialic acid as one of their carbohydrates. They are often found on or in the cell or tissue membranes and participate in a variety of biological activities.Skull: The SKELETON of the HEAD including the FACIAL BONES and the bones enclosing the BRAIN.Matrix Metalloproteinase 2: A secreted endopeptidase homologous with INTERSTITIAL COLLAGENASE, but which possesses an additional fibronectin-like domain.Matrix Metalloproteinases: A family of zinc-dependent metalloendopeptidases that is involved in the degradation of EXTRACELLULAR MATRIX components.Fractures, Bone: Breaks in bones.Femur: The longest and largest bone of the skeleton, it is situated between the hip and the knee.Collagen: A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).Bone Morphogenetic Proteins: Bone-growth regulatory factors that are members of the transforming growth factor-beta superfamily of proteins. They are synthesized as large precursor molecules which are cleaved by proteolytic enzymes. The active form can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins.Alkaline Phosphatase: An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.1.Osseointegration: The growth action of bone tissue as it assimilates surgically implanted devices or prostheses to be used as either replacement parts (e.g., hip) or as anchors (e.g., endosseous dental implants).Tibia: The second longest bone of the skeleton. It is located on the medial side of the lower leg, articulating with the FIBULA laterally, the TALUS distally, and the FEMUR proximally.X-Ray Microtomography: X-RAY COMPUTERIZED TOMOGRAPHY with resolution in the micrometer range.Bone Diseases, MetabolicBone Cysts: Benign unilocular lytic areas in the proximal end of a long bone with well defined and narrow endosteal margins. The cysts contain fluid and the cyst walls may contain some giant cells. Bone cysts usually occur in males between the ages 3-15 years.Periosteum: Thin outer membrane that surrounds a bone. It contains CONNECTIVE TISSUE, CAPILLARIES, nerves, and a number of cell types.Nuclear Matrix: The residual framework structure of the CELL NUCLEUS that maintains many of the overall architectural features of the cell nucleus including the nuclear lamina with NUCLEAR PORE complex structures, residual CELL NUCLEOLI and an extensive fibrogranular structure in the nuclear interior. (Advan. Enzyme Regul. 2002; 42:39-52)Bone Morphogenetic Protein 2: A potent osteoinductive protein that plays a critical role in the differentiation of osteoprogenitor cells into OSTEOBLASTS.Bone Marrow Transplantation: The transference of BONE MARROW from one human or animal to another for a variety of purposes including HEMATOPOIETIC STEM CELL TRANSPLANTATION or MESENCHYMAL STEM CELL TRANSPLANTATION.Haversian System: A circular structural unit of bone tissue. It consists of a central hole, the Haversian canal through which blood vessels run, surrounded by concentric rings, called lamellae.Implants, Experimental: Artificial substitutes for body parts and materials inserted into organisms during experimental studies.Parietal Bone: One of a pair of irregularly shaped quadrilateral bones situated between the FRONTAL BONE and OCCIPITAL BONE, which together form the sides of the CRANIUM.Tomography, X-Ray: Tomography using x-ray transmission.Osteonectin: Non-collagenous, calcium-binding glycoprotein of developing bone. It links collagen to mineral in the bone matrix. In the synonym SPARC glycoprotein, the acronym stands for Secreted Protein, Acidic and Rich in Cysteine.Cartilage: A non-vascular form of connective tissue composed of CHONDROCYTES embedded in a matrix that includes CHONDROITIN SULFATE and various types of FIBRILLAR COLLAGEN. There are three major types: HYALINE CARTILAGE; FIBROCARTILAGE; and ELASTIC CARTILAGE.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Matrix Metalloproteinase 1: A member of the metalloproteinase family of enzymes that is principally responsible for cleaving FIBRILLAR COLLAGEN. It can degrade interstitial collagens, types I, II and III.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Ossification, Heterotopic: The development of bony substance in normally soft structures.Collagen Type I: The most common form of fibrillar collagen. It is a major constituent of bone (BONE AND BONES) and SKIN and consists of a heterotrimer of two alpha1(I) and one alpha2(I) chains.Matrix Metalloproteinase Inhibitors: Compounds that inhibit the enzyme activity or activation of MATRIX METALLOPROTEINASES.Diphosphonates: Organic compounds which contain P-C-P bonds, where P stands for phosphonates or phosphonic acids. These compounds affect calcium metabolism. They inhibit ectopic calcification and slow down bone resorption and bone turnover. Technetium complexes of diphosphonates have been used successfully as bone scanning agents.Decalcification Technique: Removal of minerals from bones during bone examination.Osteoporosis: Reduction of bone mass without alteration in the composition of bone, leading to fractures. Primary osteoporosis can be of two major types: postmenopausal osteoporosis (OSTEOPOROSIS, POSTMENOPAUSAL) and age-related or senile osteoporosis.Ricinus: A plant genus of the family EUPHORBIACEAE, order Euphorbiales, subclass Rosidae. The seed of Ricinus communis L. is the CASTOR BEAN which is the source of CASTOR OIL; RICIN; and other lectins.Osteolysis: Dissolution of bone that particularly involves the removal or loss of calcium.Matrix Metalloproteinase 3: An extracellular endopeptidase of vertebrate tissues similar to MATRIX METALLOPROTEINASE 1. It digests PROTEOGLYCAN; FIBRONECTIN; COLLAGEN types III, IV, V, and IX, and activates procollagenase. (Enzyme Nomenclature, 1992)Fracture Healing: The physiological restoration of bone tissue and function after a fracture. It includes BONY CALLUS formation and normal replacement of bone tissue.Mandible: The largest and strongest bone of the FACE constituting the lower jaw. It supports the lower teeth.Bone Morphogenetic Protein 3: A bone morphogenetic protein that is found at high concentrations in a purified osteoinductive protein fraction from BONE. Bone morphogenetic protein 3 is referred to as osteogenin, however it may play a role in variety of developmental processes.Durapatite: The mineral component of bones and teeth; it has been used therapeutically as a prosthetic aid and in the prevention and treatment of osteoporosis.Minerals: Native, inorganic or fossilized organic substances having a definite chemical composition and formed by inorganic reactions. They may occur as individual crystals or may be disseminated in some other mineral or rock. (Grant & Hackh's Chemical Dictionary, 5th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Humerus: Bone in humans and primates extending from the SHOULDER JOINT to the ELBOW JOINT.Tissue Scaffolds: Cell growth support structures composed of BIOCOMPATIBLE MATERIALS. They are specially designed solid support matrices for cell attachment in TISSUE ENGINEERING and GUIDED TISSUE REGENERATION uses.Transforming Growth Factor beta: A factor synthesized in a wide variety of tissues. It acts synergistically with TGF-alpha in inducing phenotypic transformation and can also act as a negative autocrine growth factor. TGF-beta has a potential role in embryonal development, cellular differentiation, hormone secretion, and immune function. TGF-beta is found mostly as homodimer forms of separate gene products TGF-beta1, TGF-beta2 or TGF-beta3. Heterodimers composed of TGF-beta1 and 2 (TGF-beta1.2) or of TGF-beta2 and 3 (TGF-beta2.3) have been isolated. The TGF-beta proteins are synthesized as precursor proteins.Microscopy, Electron, Scanning: Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.Diaphyses: The shaft of long bones.Tissue Engineering: Generating tissue in vitro for clinical applications, such as replacing wounded tissues or impaired organs. The use of TISSUE SCAFFOLDING enables the generation of complex multi-layered tissues and tissue structures.Immunohistochemistry: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.Temporal Bone: Either of a pair of compound bones forming the lateral (left and right) surfaces and base of the skull which contains the organs of hearing. It is a large bone formed by the fusion of parts: the squamous (the flattened anterior-superior part), the tympanic (the curved anterior-inferior part), the mastoid (the irregular posterior portion), and the petrous (the part at the base of the skull).Alendronate: A nonhormonal medication for the treatment of postmenopausal osteoporosis in women. This drug builds healthy bone, restoring some of the bone loss as a result of osteoporosis.Biocompatible Materials: Synthetic or natural materials, other than DRUGS, that are used to replace or repair any body TISSUES or bodily function.Core Binding Factor Alpha 1 Subunit: A transcription factor that dimerizes with CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. It contains a highly conserved DNA-binding domain known as the runt domain and is involved in genetic regulation of skeletal development and CELL DIFFERENTIATION.Cathepsin K: A cysteine protease that is highly expressed in OSTEOCLASTS and plays an essential role in BONE RESORPTION as a potent EXTRACELLULAR MATRIX-degrading enzyme.Osteogenesis Imperfecta: COLLAGEN DISEASES characterized by brittle, osteoporotic, and easily fractured bones. It may also present with blue sclerae, loose joints, and imperfect dentin formation. Most types are autosomal dominant and are associated with mutations in COLLAGEN TYPE I.Calcium Phosphates: Calcium salts of phosphoric acid. These compounds are frequently used as calcium supplements.Stress, Mechanical: A purely physical condition which exists within any material because of strain or deformation by external forces or by non-uniform thermal expansion; expressed quantitatively in units of force per unit area.RANK Ligand: A transmembrane protein belonging to the tumor necrosis factor superfamily that specifically binds RECEPTOR ACTIVATOR OF NUCLEAR FACTOR-KAPPA B and OSTEOPROTEGERIN. It plays an important role in regulating OSTEOCLAST differentiation and activation.Mesenchymal Stromal Cells: Bone-marrow-derived, non-hematopoietic cells that support HEMATOPOETIC STEM CELLS. They have also been isolated from other organs and tissues such as UMBILICAL CORD BLOOD, umbilical vein subendothelium, and WHARTON JELLY. These cells are considered to be a source of multipotent stem cells because they include subpopulations of mesenchymal stem cells.Time Factors: Elements of limited time intervals, contributing to particular results or situations.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Etidronic Acid: A diphosphonate which affects calcium metabolism. It inhibits ectopic calcification and slows down bone resorption and bone turnover.Alveolar Process: The thickest and spongiest part of the maxilla and mandible hollowed out into deep cavities for the teeth.Parathyroid Hormone: A polypeptide hormone (84 amino acid residues) secreted by the PARATHYROID GLANDS which performs the essential role of maintaining intracellular CALCIUM levels in the body. Parathyroid hormone increases intracellular calcium by promoting the release of CALCIUM from BONE, increases the intestinal absorption of calcium, increases the renal tubular reabsorption of calcium, and increases the renal excretion of phosphates.Matrix Metalloproteinase 13: A secreted matrix metalloproteinase that plays a physiological role in the degradation of extracellular matrix found in skeletal tissues. It is synthesized as an inactive precursor that is activated by the proteolytic cleavage of its N-terminal propeptide.Awards and PrizesBiomechanical Phenomena: The properties, processes, and behavior of biological systems under the action of mechanical forces.Mice, Inbred C57BLMatrix Metalloproteinase 14: A transmembrane domain-containing matrix metalloproteinase. It is synthesized as an inactive zymogen that is activated by the action of PROPROTEIN CONVERTASES such as FURIN. Matrix metalloproteinase 14 plays a direct role in the cleavage of proteins in the pericellular environment. In addition, it can function indirectly by enzymatically activating the proprotein form of MATRIX METALLOPROTEINASE 15.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Matrix Metalloproteinase 7: The smallest member of the MATRIX METALLOPROTEINASES. It plays a role in tumor progression.Alveolar Bone Loss: Resorption or wasting of the tooth-supporting bone (ALVEOLAR PROCESS) in the MAXILLA or MANDIBLE.Disease Models, Animal: Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.Hydroxyproline: A hydroxylated form of the imino acid proline. A deficiency in ASCORBIC ACID can result in impaired hydroxyproline formation.Bone Cements: Adhesives used to fix prosthetic devices to bones and to cement bone to bone in difficult fractures. Synthetic resins are commonly used as cements. A mixture of monocalcium phosphate, monohydrate, alpha-tricalcium phosphate, and calcium carbonate with a sodium phosphate solution is also a useful bone paste.Osteopetrosis: Excessive formation of dense trabecular bone leading to pathological fractures; OSTEITIS; SPLENOMEGALY with infarct; ANEMIA; and extramedullary hemopoiesis (HEMATOPOIESIS, EXTRAMEDULLARY).Models, Animal: Non-human animals, selected because of specific characteristics, for use in experimental research, teaching, or testing.Porosity: Condition of having pores or open spaces. This often refers to bones, bone implants, or bone cements, but can refer to the porous state of any solid substance.Wound Healing: Restoration of integrity to traumatized tissue.Polyurethanes: A group of thermoplastic or thermosetting polymers containing polyisocyanate. They are used as ELASTOMERS, as coatings, as fibers and as foams.Fibronectins: Glycoproteins found on the surfaces of cells, particularly in fibrillar structures. The proteins are lost or reduced when these cells undergo viral or chemical transformation. They are highly susceptible to proteolysis and are substrates for activated blood coagulation factor VIII. The forms present in plasma are called cold-insoluble globulins.Bone Density Conservation Agents: Agents that inhibit BONE RESORPTION and/or favor BONE MINERALIZATION and BONE REGENERATION. They are used to heal BONE FRACTURES and to treat METABOLIC BONE DISEASES such as OSTEOPOROSIS.Mice, Knockout: Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.Osteosarcoma: A sarcoma originating in bone-forming cells, affecting the ends of long bones. It is the most common and most malignant of sarcomas of the bones, and occurs chiefly among 10- to 25-year-old youths. (From Stedman, 25th ed)Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Osteoprotegerin: A secreted member of the TNF receptor superfamily that negatively regulates osteoclastogenesis. It is a soluble decoy receptor of RANK LIGAND that inhibits both CELL DIFFERENTIATION and function of OSTEOCLASTS by inhibiting the interaction between RANK LIGAND and RECEPTOR ACTIVATOR OF NUCLEAR FACTOR-KAPPA B.Calcinosis: Pathologic deposition of calcium salts in tissues.Lumbar Vertebrae: VERTEBRAE in the region of the lower BACK below the THORACIC VERTEBRAE and above the SACRAL VERTEBRAE.Glycoproteins: Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.Phosphorus: A non-metal element that has the atomic symbol P, atomic number 15, and atomic weight 31. It is an essential element that takes part in a broad variety of biochemical reactions.Spine: The spinal or vertebral column.Spinal Fusion: Operative immobilization or ankylosis of two or more vertebrae by fusion of the vertebral bodies with a short bone graft or often with diskectomy or laminectomy. (From Blauvelt & Nelson, A Manual of Orthopaedic Terminology, 5th ed, p236; Dorland, 28th ed)Bone Morphogenetic Protein 7: A bone morphogenetic protein that is widely expressed during EMBRYONIC DEVELOPMENT. It is both a potent osteogenic factor and a specific regulator of nephrogenesis.Matrix Metalloproteinases, Membrane-Associated: Matrix metalloproteinases that are associated with the CELL MEMBRANE, either through transmembrane domains or GLYCOSYLPHOSPHATIDYLINOSITOL ANCHORS. Membrane-type matrix metalloproteinases may act within the pericellular environment to influence the process of CELL MIGRATION.

Noncollagenous bone matrix proteins, calcification, and thrombosis in carotid artery atherosclerosis. (1/363)

Advanced atherosclerosis is often associated with dystrophic calcification, which may contribute to plaque rupture and thrombosis. In this work, the localization and association of the noncollagenous bone matrix proteins osteonectin, osteopontin, and osteocalcin with calcification, lipoproteins, thrombus/hemorrhage (T/H), and matrix metalloproteinases (MMPs) in human carotid arteries from endarterectomy samples have been determined. According to the recent American Heart Association classification, 6 of the advanced lesions studied were type V (fibroatheroma) and 16 type VI (complicated). Osteonectin, osteocalcin, and osteopontin were identified by monoclonal antibodies IIIA(3)A(8), G12, and MPIIIB10(1) and antiserum LF-123. Apolipoprotein (apo) AI, B, and E; lipoprotein(a); fibrinogen; fibrin; fragment D/D-dimer; MMP-2 (gelatinase A); and MMP-3 (stromelysin-1) were identified with previously characterized antibodies. Calcium phosphate deposits (von Kossa's stain) were present in 82% of samples (3 type V and 15 type VI). Osteonectin was localized in endothelial cells, SMCs, and macrophages and was associated with calcium deposits in 33% of type V and 88% of type VI lesions. Osteopontin was distributed similarly to osteonectin and was associated with calcium deposits in 50% of type V and 94% of type VI lesions. Osteocalcin was localized in large calcified areas only (in 17% of type V and 38% of type VI lesions). ApoB colocalized with cholesterol crystals and calcium deposits. Lipoprotein(a) was localized in the intima, subintima, and plaque shoulder. Fibrin (T/H) colocalized with bone matrix proteins in 33% of type V and 69% of type VI lesions. MMP-3 was cytoplasmic in most cells and colocalized with calcium and fibrin deposits. MMP-2 was less often associated with calcification. The results of this study show that osteonectin, osteopontin, and osteocalcin colocalized with calcium deposits with apoB, fibrin, and MMP-3 in advanced, symptomatic carotid lesions. These data suggest that the occurrence of T/H might contribute to dystrophic arterial calcification in the progression and complications of atherosclerosis.  (+info)

Xenogenic demineralized bone matrix: osteoinduction and influence of associated skeletal defects in heterotopic bone formation in rats. (2/363)

Demineralized bone matrix (DBM) was ectopically implanted in 36 male Wistar rats. In 18 of the animals a bone defect in the femoral condyles was also created: the left was filled with DBM and the right was left empty as a control. The animals were killed after 2, 4 and 6 weeks and new bone was histologically evaluated, comparing ectopic bone formation with or without distant bone injury. Results showed: (1) osteoinductivity of xenogenic DBM, and (2) earlier mineralization of ectopically implanted DBM in the group with associated skeletal injury. Our results show that xenogenic bone matrix acts as an osteoinductive material and that skeletal injury improves osteogenesis at distant sites.  (+info)

Changes in the orientation of collagen fibers on the superficial layer of the mouse tibial bone after denervation: scanning electron microscopic observations. (3/363)

This study was undertaken to evaluate the relationship between the mechanical stress loaded onto the bone and the orientation of collagen fibers formed by osteoblasts. The femoral, obturator, and sciatic nerves in the left posterior legs of 7-week-old mice were exposed and electroscissored to reduce the mechanical stress loaded onto the leg. Four weeks after operation, the tibial bones in the control and denervated legs were removed and observed by scanning electron microscopy (SEM) after NaOCl treatment. In the control right tibia, collagen fibers on the superficial bone matrix tended to be arranged parallel to the longitudinal axis of the bone. However, the arrangement of collagen fibers in the left tibia, which were immobilized for 4 weeks by denervation, was disorganized and ran in random directions. The findings suggest that the direction of collagen fibers in the bone changes in response to the mechanical stress loaded onto the bone, probably due to changes in the activity of osteoblasts in the denervated leg.  (+info)

Bone morphogenetic proteins in human bone regeneration. (4/363)

Recently, the first clinical reports on bone regeneration by two recombinant human bone morphogenetic proteins (rhBMPs), BMP-2 and BMP-7 (also named osteogenic protein-1, OP-1) have been published (1-4). Although both BMPs were able to support bone regeneration, a significant variation in individual response was observed with both proteins. Animal studies and laboratory experiments reveal a number of conditions that influence the osteoinductivity of BMP, such as BMP concentration, carrier properties and influence of local and systemic growth factors and hormones. In this paper, these studies and the clinical reports are reviewed, and the conditions that modulate the BMP-dependent osteoinduction are discussed. The information may provide clues as to how the performance of recombinant human BMP as bone-graft substitute in humans can be improved.  (+info)

The cell biology of osteoclast function. (5/363)

Osteoclasts are multinucleated cells responsible for bone resorption. They have developed an efficient machinery for dissolving crystalline hydroxyapatite and degrading organic bone matrix rich in collagen fibers. When initiating bone resorption, osteoclasts become polarized, and three distinct membrane domains appear: a ruffled border, a sealing zone and a functional secretory domain. Simultaneously, the cytoskeleton undergoes extensive re-organisation. During this process, the actin cytoskeleton forms an attachment ring at the sealing zone, the membrane domain that anchors the resorbing cell to bone matrix. The ruffled border appears inside the sealing zone, and has several characteristics of late endosomal membrane. Extensive vesicle transport to the ruffled border delivers hydrochloric acid and proteases to an area between the ruffled border and the bone surface called the resorption lacuna. In this extracellular compartment, crystalline hydroxyapatite is dissolved by acid, and a mixture of proteases degrades the organic matrix. The degradation products of collagen and other matrix components are endocytosed, transported through the cell and exocytosed through a functional secretory domain. This transcytotic route allows osteoclasts to remove large amounts of matrix-degradation products without losing their tight attachment to underlying bone. It also facilitates further processing of the degradation products intracellularly during the passage through the cell.  (+info)

109Cd K x ray fluorescence measurements of tibial lead content in young adults exposed to lead in early childhood. (6/363)

OBJECTIVES: Tibia lead measurements were performed in a population of 19-29 year old people who had been highly exposed to lead in childhood to find whether lead had persisted in the bone matrix until adulthood. METHODS: (109)Cd K x ray fluorescence was used to measure the tibia lead concentrations of 262 exposed subjects and 268 age and sex matched controls. Questionnaire data allowed a years of residence index to be calculated for exposed subjects. A cumulative blood lead index was calculated from the time weighted integration of available data of blood lead. RESULTS: The mean (SEM) difference between exposed and control men was 4.51 (0.35) micrograms Pb/g bone mineral, and between exposed and control women was 3.94 (0. 61) micrograms Pb/g bone mineral. Grouped mean bone lead concentrations of exposed subjects were predicted best by age. When exposed and control subjects' data were combined, grouped mean bone lead concentrations were predicted best by cumulative blood lead index. The years of residence index was neither a good predictor of bone lead concentrations for exposed subjects nor for exposed and control subjects combined. Finally, exposed subjects had increased current blood lead concentrations that correlated significantly with bone lead values. CONCLUSION: Bone lead concentrations of exposed subjects were significantly increased compared with those of control subjects. Lead from exposure in early childhood had persisted in the bone matrix until adulthood. Exposed subjects had increased blood lead concentrations compared with controls. Some of this exposure could be related to ongoing exposure. However, some of the increase in blood lead concentration in adult exposed subjects seemed to be a result of endogenous exposure from increased bone lead stores. The endogenous exposure relation found for men was consistent with reported data, but the relation found for women was significantly lower. Further research is needed to find whether the observed differences are due to sex, or pregnancy and lactation.  (+info)

The roles of annexins and types II and X collagen in matrix vesicle-mediated mineralization of growth plate cartilage. (7/363)

Annexins II, V, and VI are major components of matrix vesicles (MV), i.e. particles that have the critical role of initiating the mineralization process in skeletal tissues. Furthermore, types II and X collagen are associated with MV, and these interactions mediated by annexin V stimulate Ca(2+) uptake and mineralization of MV. However, the exact roles of annexin II, V, and VI and the interaction between annexin V and types II and X collagen in MV function and initiation of mineralization are not well understood. In this study, we demonstrate that annexin II, V, or VI mediate Ca(2+) influx into phosphatidylserine (PS)-enriched liposomes, liposomes containing lipids extracted from authentic MV, and intact authentic MV. The annexin Ca(2+) channel blocker, K-201, not only inhibited Ca(2+) influx into fura-2-loaded PS-enriched liposomes mediated by annexin II, V, or VI, but also inhibited Ca(2+) uptake by authentic MV. Types II and X collagen only bound to liposomes in the presence of annexin V but not in the presence of annexin II or VI. Binding of these collagens to annexin V stimulated its Ca(2+) channel activities, leading to an increased Ca(2+) influx into the liposomes. These findings indicate that the formation of annexin II, V, and VI Ca(2+) channels in MV together with stimulation of annexin V channel activity by collagen (types II and X) binding can explain how MV are able to rapidly take up Ca(2+) and initiate the formation of the first crystal phase.  (+info)

Localization of alkaline phosphatase and osteopontin during matrix mineralization in the developing cartilage of coccygeal vertebrae. (8/363)

We observed the manner in which alkaline phosphatase (ALPase) and osteopontin were localized in the cartilage and intramembranous bone of coccygeal vertebrae during matrix mineralization, shedding considerable light on the manner in which they develop. In the cartilage matrix of coccygeal vertebrae, we observed the localization of ALPase activity in the boundary of the proliferative and the hypertrophic zones. Granular nodules of mineralization were consistently found in the boundary of both zones, and increased in size when close to the hypertrophic zone. While osteopontin was rarely present in the early stages of mineralization, its localization along the margins of mineralized matrices in the hypertrophic zone was prominent. In contrast to cartilage, mineralized nodules in the intramembranous bone in the mid-portion of the vertebra displayed osteopontin-immunoreactivity, indicating its early synthesis and subsequent accumulation to early-stage mineralized nodules. When blood vessels, accompanied by osteoblastic and osteoclastic cell populations, invaded the cartilage, osteopontin was localized in the lower region of the hypertrophic zone, despite its maintaining the localization of ALPase and early-stage mineralization. Thus, our investigation demonstrated ALPase activity consistent with early-stage mineralization in the cartilage matrix. However, the fact that osteopontin-localization could not be pinpointed might account for its multifunctionality as concerns both the regulation of mineralization and the attachment of migrating osteogenic and osteoclastic cells to the mineralized matrix.  (+info)

Demineralized bone matrix fibers and a demineralized bone matrix composition are provided. The demineralized bone matrix fibers have an average fiber length in the range from about 250 μm to about 2 mm and an aspect ratio of greater than about 4. The demineralized bone matrix composition includes demineralized bone matrix fibers and a biocompatible liquid in an amount to produce a coherent, formable mass. The formable mass retains its cohesiveness when immersed in a liquid. Methods for making the demineralized bone matrix fibers and composition are also provided.
A comparison of bone matrix and tendon with particular reference to glycoprotein content.: Bone matrix and tendon are compared in terms of their carbohydrate an
Inhibition of PPAR gamma 2 by BADGE and Vitamin D in Male Mice Increases Osteoblastogenesis and Inhibits Bone Matrix Mineralization Leading to Osteomalacia. Conference Proceedings ...
Today, DePuy Synthes*, in collaboration with LifeNet Health , launches ViviGen Formable Cellular Bone Matrix**, a second generation cellular allograft used to assist in the formation
The extracellular matrix (ECM) has been viewed as a static three dimensional scaffold that supports cells and tissues. However, our recent molecular imaging stu...
DePuy Synthes, part of J&J, announced the release of its CONFORM SHEET demineralized cancellous bone scaffold thats designed for implantation during
The spines in Group I demonstrated higher rates of radiographically evident fusion at eight weeks than did the spines in Group III or Group IV (p , 0.05). Manual testing of the spines at four weeks revealed variable fusion rates (five of six in Group I, two of six in Group II, and none of six in Group Ill). At eight weeks, all six spines in Group I, three of the six in Group II, and no spine in Group III or IV had fused. Histologic analysis of the spines in Groups I, II, and III demonstrated varying amounts of residual demineralized bone matrix and new bone formation. Group-1 spines demonstrated the most new bone formation. ...
Hi I have some skeletal animation that is driven in the vertex shader, each vertex has a selection of bones that influence it and a bone matrix is passed representing all the bones that can influence and the eventual result is a perfect skeleton. This is a simple matrix multiplication in the shader. Id like to know when my mouse cursor is selecting any of the bone meshes that are drawn as part of the skeleton. Because all the grunt work is done in the shader I dont know the model matrix
The radioactively labelled constituents present in bone matrix were compared 12 days after injection of either [(14)C]glucosamine or plasma [(14)C]glycoprotein. Both precursors are utilized in the synthesis of organic matrix by bone tissue. Cortical bone from animals injected with [(14)C]glucosamine contains radioactivity derived from glucosamine and plasma glycoproteins and all glycoprotein fractions are labelled. Plasma [(14)C]glycoprotein labels the less acidic glycoproteins to a greater extent than the more acidic components. An antibody has been raised against the less-acidic-glycoprotein fraction of bone. The latter contains a glycoprotein of alpha-mobility that appears to be concentrated specifically in bone tissue and which is present also in plasma. This alpha-glycoprotein accounts for a large proportion of the components labelled and retained in bone matrix after [(14)C]glucosamine injection.
TY - JOUR. T1 - The effects of cytokines and growth factors on osteoblastic cells. AU - Mundy, G. R.. AU - Boyce, B.. AU - Hughes, D.. AU - Wright, K.. AU - Bonewald, L.. AU - Dallas, S.. AU - Harris, S.. AU - Ghosh-Choudhury, N.. AU - Chen, D.. AU - Dunstan, C.. AU - Izbicka, E.. AU - Yoneda, T.. PY - 1995/8. Y1 - 1995/8. N2 - In this short review, some regulatory mechanisms that are involved in the control of normal bone formation are proposed, based on several in vivo and in vitro models our group has utilized recently to study osteoblast differentiation and mineralized bone matrix formation. Of course, these proposals must be assessed in the light of the limitations of the models, which probably represent a simplification of the complex and different ways in which normal mammalian bone is formed at different sites. Nevertheless, it is likely that the same general types of control mechanisms are active in each of the different types of bone formation. In adult humans, bone formation ...
It is not possible to say if this is a direct effect of estrogens on bone or the result of secondary effects but it suggests that in transsexuals receiving high dose estrogen therapy there is a decrease in bone matrix formation.
A demineralized bone matrix is produced by a process in which a bone body is placed in a first processing solution comprising an acid to demineralize the bone body. The bone body is periodically removed from the first solution at specific time intervals to perform at least one test, such as a compression test, on a mechanical property of the bone body. When the test yields a desired result, the bone body is exposed to a second processing solution that is less acidic than the first, thus minimizing the exposure of the bone body to the harsh acidic conditions of the demineralization phase of the process.
This animal experiment compared the regenerative processes within defined bony defects of the porcine skull after delivery of routinely utilized bone graft materials: anorganic bone matrix (ABM) and an identical ABM carrying the cell binding peptide P-15. Particulated autogenous bone was used as a c …
Osteoblasts are responsible for deposition of the mineralized bone matrix. Osteoblasts attach to the surface of the bone matrix, proliferate, and secrete type I collagen and bone-specific non-collagenous proteins such as osteocalcin. These proteins are crucial for mineralization of the bone matrix ...
Results: At the time of sacrifice, statistically significant differences between the four types of defects in the total area of bone regeneration, percentage of neoformed bone matrix, medullary space, or contact between particulate biomaterial and neoformed bone matrix were not found. All defects showed a significant increase in neoformed bone matrix as sacrifice was delayed, but a uniform pattern was not followed. Only defects treated with ß-TCP-Fn-ADSCs showed a significant increase in the bone regeneration area when animals sacrificed at 3 months were compared to those sacrificed at 1 month (P = .006 ...
August 18, 2008 - ETEX Corp. launched EquivaBone, a bone graft substitute that remodels and is replaced with new bone during the healing process.. EquivaBone is a proprietary combination of osteoinductive Demineralized Bone Matrix (DBM) and ETEXs osteoconductive nanocrystalline calcium phosphate. ETEX now offers reportedly the first combination product that is both moldable and injectable and sets hard once implanted. In addition, osteoinductivity of the DBM is certified after sterilization, by lot, for the entirety of shelf life.. Both trauma and spine fusion data were included in the 510k submission which received FDA clearance on April 28, 2008. EquivaBone is intended for use in filling bone voids or defects of the skeletal system (including extremities, pelvis, and spine) that are not intrinsic to the stability of the bone structure.. For more information: www.etexcorp.com. ...
1 uM dexamethasone, 10 mM B glycerolphosphate and 50 uM ascorbic new post acid for 14 days. The in duction medium was changed every 3 days, and the bone matrix mineralization was evaluated by Alizarin red S staining. The ARS was ex tracted by adding 10% cetylpyridinium chloride in 8 mM Na2HPO4 and 1. 5 mM KH2PO4 and the absorbance was mea sured by SpectraMax 190 ELISA plate reader at 550 nm. Cell proliferation assay To evaluate the cell proliferation, MTT 2,5 diphenyl 2H tetrazoliumbromide assay was performed as described previously. Briefly, cells were seeded at the density of 1. 5 103 cells/well in 96 well plate and cultured without or with various concentrations of OGT2115. Cells were analyzed every two days by adding Inhibitors,Modulators,Libraries 10 uL of the MTT to each well and the cells were continued to culture for 4 hr.. After the incubation, the supernatant was discarded and 100 uL of dimethyl sulfoxide was added to each well to dissolve the formazan. The number of cells was ...
... ,Demineralized bone matrix dispensed in a syringe. DBM will be free of foreign matter. Demineralized bone matrix particle size 125 to 850 . Glycerol 70-88% by weight. Calcium content less than 8%.,medicine,medical supply,medical supplies,medical product
The miniaturization of the current technology of storage media is hindered by fundamental limits of quantum mechanics. A new approach consists in using so-called spin-crossover molecules as the smallest possible storage unit. Similar to normal hard drives, these special molecules can save information via their magnetic state. A research team from Kiel University has now managed to successfully place a new class of spin-crossover molecules onto a surface and to improve the molecules storage capacity. The storage density of conventional hard drives could therefore theoretically be increased by more than one hundred fold. The study has been published in the scientific journal Nano Letters. ...
90% of bone matrix proteins are made of collagen, which is crucial for bone strength just as much as calcium. Read about vegetarian protein sources here!
Affiliation:Okayama University Dental School,歯学部,助教授, Research Field:Morphological basic dentistry, Keywords:Immunohistochemistry,免疫組織化学,骨芽細胞,初期石灰化,歯原性腫瘍,Cementum,Bone matrix protein,Pathogenesis,口腔粘膜上皮,コラーゲン, # of Research Projects:6, # of Research Products:0
Matrix OI is a compressible stem cell containment matrix that is derived from 100% human bone. Matrix OI is 100% lot to lot tested for osteoinductivity post sterilization to ensure the presence of bone morphogenic protiens and growth factors. BMPs promote mesenchymal (BMA) cells to differentiate into chondrocytes and osteoblasts that lead to bone formation. Preserved native BMPs provide for an unparalleled osteoactive grafting material. Matrix OI can be hydrated with the patients own stem cells, BMA, growth factors, PRP or with an antibiotic solution.. Matrix OI is indicated for use in spine surgeries, scoliosis, lateral gutters, orthopedics, bone voids, reconstruction, CMF, non-unions, foot and ankle, and dental procedures. When hydrated, matrix OI is a compressable scaffold that will contour with the defect.. ...
View indications, safety, and warnings for Grafton Bone Graft, a demineralized bone matrix (DBM), used with oral-maxillofacial surgery.
Filion TM, Li X, Mason-Savas A, Kreider JM, Goldstein SA, Ayers DC, Song J. Elastomeric osteoconductive synthetic scaffolds with acquired osteoinductivity expedite the repair of critical femoral defects in rats. Tissue Eng Part A. 2011 Feb; 17(3-4):503-11 ...
TY - JOUR. T1 - The role of a composite, demineralized bone matrix and bone marrow in the treatment of osseous defects. AU - Tiedeman, J. J.. AU - Garvin, K. L.. AU - Kile, T. A.. AU - Connolly, J. F.. PY - 1995/1/1. Y1 - 1995/1/1. N2 - The efficacy of demineralized bone matrix (DBM) used alone and with bone marrow as a graft material in the treatment of osseous defects was evaluated in 48 patients. Of these 48 patients, 39 were available to follow up and review. Follow up averaged 19 months for all patients. In the entire series, 30 of 39 patients demonstrated osseous union for a 77% success rate. Patients with fracture nonunion represented the most recalcitrant group clinically, with union achieved in only 61% of these cases. Overall, the 39 patients grafted with DBM demonstrated healing that was comparable to results achieved with standard lilac crest bone graft. The results indicate the DBM and marrow composite grafting is a suitable alternative to autologous iliac crest bone graft for use ...
DOI: 10.11607/prd.00.0510 The effect of the pure-phase beta-tricalcium phosphate (beta-TCP) Cerasorb on bone regeneration was evaluated in hollow titanium cylinders implanted in the posterior jaws of five volunteers. Beta-TCP particles were inserted inside the cylinders and harvested 6 months after placement. The density of the newly formed bone inside the bone-growing chambers measured 27.84% 24.67% in test and 17.90% 4.28% in control subjects, without a statistically significant difference. Analysis of the histologic specimens revealed that the density of the regenerated bone was related to the density of the surrounding bone. The present study demonstrates the spontaneous healing of infrabony artificial defects, 2.5 mm diameter, in the jaw. The pure beta-TCP was resorbed simultaneously with new bone formation, without interference with the bone matrix formation. (Int J Periodontics Restorative Dent 2003;23:69 77 ...
The aim here was to explore a new graft material that excludes the need to harvest autogenous bone from patients. Forty-two critical-size (10 x 15 mm) defects were created in rabbit mandibles bilaterally. Five groups of six defects each were grafted with autogenous endochondral (EC) bone, autogenous intramembranous (IM) bone, fresh-frozen allogeneic IM bone only, fresh-frozen allogeneic IM bone and demineralized bone matrix powder prepared from intramembranous bone (DBM IM) only, and fresh-frozen allogeneic IM bone and basic fibroblast growth factor (bFGF) mixed with DBM IM powder. The remaining defects were used as controls. Three weeks after surgery, the defects were retrieved for histological analysis. The amount of new bone formation was quantified by image analysis. No bone formed across the defect in the controls; 224% more new bone formed in defects grafted with composite allogeneic IM bone/DBM IM than in those grafted with allogeneic IM bone alone (p < 0.001); 550% more new bone was ...
The highly intertwined, cross-linked structure of collagen makes it reasonable that fragments of this molecule could survive for 68 million years. Even if the individual protein strands break down, the fiber would still remain largely intact because of all the association points. Once the protein strand breaks, the fragments are held in close proximity by the contact points. This forced closeness allows for broken strands to occasionally rejoin and reform the original protein. If the broken strands were not held juxtaposed to each other, the fragments would diffuse away from each other, thus, preventing the reversal of the degradation process.. Finally, collagens association with the bone matrix provides added stability to the collagen proteins. Within the bone matrix, collagen fibers adsorb to the mineral component of bone. The contact with the surface protects the protein and keeps the pieces of collagen juxtaposed whenever the protein strands break.. Collagens abundance further explains its ...
Remodeling a kitchen to add additional cabinets to existing ones also means adding extra support. In a similar respect, bone remodeling alters bone structure without adversely affecting bone strength. What divalent cation is required to create additional bone matrix ...
Health, ...A study conducted at the University of Granada hasdemonstrated that ib...In vitro tests demonstrated that unlike other NSAIDs when a therapeuti...Osteoblast cells are bone cells that synthesize the bone matrix. Conse...Positive Results ...,A,study,demonstrates,that,ibuprofen,improves,bone,repair,after,surgery,or,a,fracture,medicine,medical news today,latest medical news,medical newsletters,current medical news,latest medicine news
You must limit the use of your damaged limb. Relax and accept that you cannot do the things you used to normally do. If you do, try to do things too soon, you will possibly lengthen the time it takes to get back to normal. But, right now, lets talk about how you might help to speed your recovery time. Because bone is about 70% mineral content, it is high in phosphorus, magnesium, silicon, zinc, and of course, calcium, along with some other minerals. Bone is also very high in protein content. A high-protein diet will provide some of the building blocks needed to synthesize a new bone matrix.. Plant-based proteins are recommended, such as nuts, oats, and quinoa, instead of animal and dairy proteins which contain more fat. Amino acids found in protein, such as lysine, arginine, glycine, cysteine and glutamine are responsible for the absorption of calcium and the regeneration of bone tissue. Anti-oxidants, like vitamins E, C and K, may aid in fracture healing by suppressing inflammation. By adding ...
BMP3 Human Recombinant produced in E.coli is a non-glycosylated disulfide linked homodimer containing 2 chains of 110 amino acids.
Fiat is close to launching the U.S. spec Fiat 500 Abarth, and this first teaser video tries to position the car as a rebel and a bad boy.
The Fiat 500 is not new news, but stop! (collaborate and listen) there is something in this certain 500 that makes it harp back to its predecessor more than
阿伏苯宗 (英語:Avobenzone,商品名: Parsol 1789, Eusolex 9020, Escalol 517 等。INCI名稱:Butyl Methoxydibenzoylmethane) 阿伏苯宗為USAN名稱。為一使用於防曬產品中的脂溶性成分,能夠吸收所有波段的 UVA(約 315至400奈米),是二苯甲酰甲烷(DBM)的衍生物。其中,阿伏苯宗對於波長 357奈米的 UVA吸收率最高[1]。. 因為相較於其他化學防曬劑它能夠吸收相當大範圍波段的紫外光,因此被廣泛地使用於許多標榜廣譜防曬產品中。. ...
TY - JOUR. T1 - The mechanisms of uremic serum-induced expression of bone matrix proteins in bovine vascular smooth muscle cells. AU - Chen, N. X.. AU - Duan, D.. AU - ONeill, K. D.. AU - Wolisi, G. O.. AU - Koczman, J. J.. AU - LaClair, R.. AU - Moe, S. M.. PY - 2006/9/1. Y1 - 2006/9/1. N2 - We have previously found that uremic human serum upregulates RUNX2 in vascular smooth muscle cells (VSMCs), and that RUNX2 is upregulated in areas of vascular calcification in vivo. To confirm the role of RUNX2, we transiently transfected a dominant-negative RUNX2 (ΔRUNX2) construct in bovine vascular smooth muscle cells (BVSMCs). Blocking RUNX2 transcriptional activity significantly decreased uremic serum induced alkaline phosphatase (ALP) activity (268 ± 34 vs 188 ± 9.5 U/g protein, P , 0.05) and osteocalcin expression (172 ± 17 vs 125 ± 9 ODU, P , 0.05). To determine the mechanism by which uremic serum upregulates RUNX2, we examined cell signaling pathways. BVSMCs were incubated in the presence or ...
The degree of mineralization of bone matrix is an important factor in determining the mechanical competence of bone. The remodeling and modeling activities of bone cells together with the time course of mineralization of newly formed bone matrix generate a characteristic bone mineralization density distribution (BMDD). In this study we investigated the biological variance of the BMDD at the micrometer level, applying a quantitative backscattered electron imaging (qBEI) method. We used the mean calcium concentration (CaMean), the most frequent calcium concentration (CaPeak), and full width at half maximum (CaWidth) to characterize the BMDD. In none of the BMDD parameters were statistically significant differences found due to ethnicity (15 African-American vs. 27 Caucasian premenopausal women), skeletal site variance (20 ilium, 24 vertebral body, 13 patella, 13 femoral neck, and 13 femoral head), age (25 to 95 years), or gender. Additionally, the interindividual variance of CaMean and CaPeak, ...
Eur J Oral Implantol. 2013 Spring;6(1):27-37. Autogenous bone versus deproteinised bovine bone matrix in 1-stage lateral sinus floor elevation in the severely atrophied maxilla: a randomised controlled trial. Merli M1, Moscatelli M, Mariotti G, Rotundo R, Nieri M. Abstract PURPOSE: To compare 100% deproteinised bovine bone matrix (DBBM) grafts (test group) with 100% autogenous bone ...
Increases in PTH concentration in blood result in mobilization of calcium phosphate from the bone matrix due to increased osteoclastic activity. The initial phase is seen within 1-2 hr and results from activation of preformed osteoclasts already present on the bone surface. A later and more pronounced phase of the response to PTH becomes evident after about 12 hr and is characterized by widespread resorption of both mineral and organic components of bone matrix, particularly in trabecular bone. Evidence of osteoclastic activity is reflected not only by calcium phosphate mobilization, but also by increased urinary excretion of hydroxy-proline and other products of collagen breakdown.. Although activity of all bone cell types is affected by PTH, only cells of osteoblastic lineage express receptors for PTH. Osteoclasts are thus not direct targets for PTH. Activation, differentiation, and recruitment of osteoclasts in response to PTH depends on increased expression of at least two cytokines by cells ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Orthoplex Mineral Matrix 60 Tablets offers relief of muscular cramps and spasms. Orthoplex Mineral Matrix contains minerals that support bone matrix & health
Background and Objectives Monocytes can give rise to different cell types including osteoclasts (OC), which play an important role in maintaining bone homeostasis by resorbing bone matrix. Circulating monocytes consist of at least two main functional subsets of immune cells, Ly6Chigh and Ly6Clow monocytes, arising from a common progenitor in the bone marrow. Excessive and prolonged activation of inflammatory LyC6high monocytes is a hallmark of many inflammatory diseases including arthritis. Among key molecular rheostats, micro (mi) RNAs are a class of regulatory RNAs that control basic biological functions and orchestrate inflammatory responses. Among master miRNAs of innate immunity, miR-146a exerts a negative retro-control on inflammation transduction signals and inhibits osteoclastogenesis. Despite aberrant increased expression in rheumatoid arthritis (RA), miR-146a is unable to properly down regulate inflammation, leading to prolonged TNF production and increased OC, two arthritis hallmarks. ...
Fiat 131 U1112 OBD-II Diagnostic Network (U) Trouble Code Information Page. Reason for Fiat 131 U1112 and find the solution for the code Fiat 131 U1112 , well give to how to fix Fiat 131 U1112 trouble code problem.
The head of Fiat SpA said it had more than a 50 percent chance of succeeding in its bid for Opel, adding that the other contenders did not have the expertise to save the struggling car maker, according to a newspaper report.
Italys Fiat now owns most of Chryslers assets. The deal was closed today, saving the troubled U.S. automaker from liquidation.
... , Ramón Fuentes, Diego Saravia, Alain Arias1, Ruth Prieto, Fernando Dias
Serum calcium levels are tightly controlled by an integrated hormone-controlled system that involves active vitamin D [1,25(OH)2D], which can elicit calcium mobilization from bone when intestinal calcium absorption is decreased. The skeletal adaptations, however, are still poorly characterized. To gain insight into these issues, we analyzed the consequences of specific vitamin D receptor (Vdr) inactivation in the intestine and in mature osteoblasts on calcium and bone homeostasis. We report here that decreased intestinal calcium absorption in intestine-specific Vdr knockout mice resulted in severely reduced skeletal calcium levels so as to ensure normal levels of calcium in the serum. Furthermore, increased 1,25(OH)2D levels not only stimulated bone turnover, leading to osteopenia, but also suppressed bone matrix mineralization. This resulted in extensive hyperosteoidosis, also surrounding the osteocytes, and hypomineralization of the entire bone cortex, which may have contributed to the ...
0061]In certain embodiments, a device of the invention will include one or more substances, additional to the osteoinductive DBM material that induces or generates the formation of bone. Suitable osteogenic materials may include a growth factor that is effective in inducing formation of bone. Desirably, the growth factor will be from a class of proteins known generally as bone morphogenic proteins (BMPs), and may in certain embodiments be recombinant human (rh) BMPs. These BMP proteins, which are known to have osteogenic, chondrogenic and other growth and differentiation activities, include rhBMP-2, rhBMP-3, rhBMP4 (also referred to as rhBMP-2B), rhBMP-5, rhBMP-6, rhBMP-7 (rhOP-1), rhBMP-8, rhBMP-9, rhBMP-12, rhBMP-13, rhBMP-15, rhBMP-16, rhBMP-17, rhBMP-18, rhGDF-1, rhGDF-3, rhGDF-5, rhGDF-6, rhGDF-7, rhGDF-8, rhGDF-9, rhGDF-10, rhGDF-11, rhGDF-12, rhGDF-14. For example, BMP-2, BMP-3, BMP-4, BMP-5, BMP-6 and BMP-7, disclosed in U.S. Pat. Nos. 5,108,922; 5,013,649; 5,116,738; 5,106,748; ...
The bone matrix and tooth enamel are the hardest substances in the body, and both are formed through the mineralization of proteins secreted by osteoblasts and ameloblasts, respectively. One of the primary components of the bone matrix is collagen, which provides strength, as well as acts as a substrate for mineralization. Amelogenin is the main extracellular matrix protein secreted by ameloblasts and is important for both the inclusion of minerals and the proper structure of tooth enamel. Defects in the secretion or structure of these extracellular matrices compromise the strength and durability of the tissues. Two studies reveal distinct mechanisms of secretory dysregulation that affect proper mineralization of these tissues. Zhao et al. found that the trimeric intracellular cation channel Tric-b, which localizes to the endoplasmic reticulum (ER), was important to secretion of collagen by osteoblasts in mice. Mutations in TMEM38b, the human homolog of Tric-b, are associated with osteogenesis ...
The purpose of this study is to determine if Ixmyelocel-T grafting with demineralized bone matrix bound in autologous plasma after core decompression surgery is superior to core decompression with demineralized bone matrix bound in autologous plasma in preventing progression of osteonecrosis to a more severe disease stage (Stage II to III or higher) from the time of surgery until 24 months later, in patients with University of Pennsylvania (UPenn) Stage IIB or C disease at diagnosis ...
Our main finding was that the AMIC technique with MaioRegen implant failed in 31% of our patients who required re-intervention with implantation of ankle prostheses, although we found a progressive significant improvement of AOFAS, VAS and MOCART scores overall.. It is not easy to compare our results to those of previous studies focusing on other surgical treatments of OLTs, since different criteria for considering successful outcomes can be encountered. Chuckpaiwong et al. studied 105 patients with OLT treated with arthroscopic debridement with osteochondral bone stimulation (microfracture) [23]. They observed a successful rate of 70% with all unsuccessful treatments involving patients with lesions greater than 15 mm [23]. Ferkel and colleagues evaluated the long-term (average follow-up: 71 months) results of patients with chronic symptomatic OLT treated predominantly by arthroscopic excision and drilling ankle. They reported good to excellent results in 64% of patients based on the modified ...
Bone is the most common site of metastasis for breast cancer, however the reasons for this remain unclear. We hypothesise that under certain conditions mammary cells possess osteomimetic capabilities that may allow them to adapt to, and flourish within, the bone microenvironment. Mammary cells are known to calcify within breast tissue and we have recently reported a novel in vitro model of mammary mineralization using murine mammary adenocarcinoma 4T1 cells. In this study, the osteomimetic properties of the mammary adenocarcinoma cell line and the conditions required to induce mineralization were characterized extensively. It was found that exogenous organic phosphate and inorganic phosphate induce mineralization in a dose dependent manner in 4T1 cells. Ascorbic acid and dexamethasone alone have no effect. 4T1 cells also show enhanced mineralization in response to bone morphogenetic protein 2 in the presence of phosphate supplemented media. The expression of several bone matrix proteins were monitored
Studies of biological systems typically require the application of several complementary methods able to yield statistically-relevant results at a unique level of sensitivity. Combined X-ray fluorescence and ptychography offer excellent elemental and structural imaging contrasts at the nanoscale. They enable a robust correlation of elemental distributions with respect to the cellular morphology. Here we extend the applicability of the two modalities to higher X-ray excitation energies, permitting iron mapping. Using a long-range scanning setup, we applied the method to two vital biomedical cases. We quantified the iron distributions in a population of macrophages treated with Mycobacterium-tuberculosis-targeting iron-oxide nanocontainers. Our work allowed to visualize the internalization of the nanocontainer agglomerates in the cytosol. From the iron areal mass maps, we obtained a distribution of antibiotic load per agglomerate and an average areal concentration of nanocontainers in the agglomerates. In
Iron is an essential content that helps the body to protect against Anemia, raising body metabolism that leads to weight loss.. Pregnant woman is advised to take palak by the doctors for babys health and breast feeding. The folate in pasalai keerai enhances the healthy growth of nervous system, lungs growth in the fetus.. To protect the bones from osteoporosis, doctors recommend to take palak at least one a week. The minerals like magnesium, copper, zinc, manganese and phosphorus creates strong bones and vitamin K helps to retain calcium in bone matrix and helps in bone mineralization.. Indian spinach is good for diabetes. It acts as a agent to destroy the unwanted toxic substances in the stomach and it helps to reduce the blood sugar levels by excess urination. The magnesium is the needed mineral to maintain the blood sugar level from too much fluctuation. The best medicine for constipation.. The cancer preventing components like fiber, chlorophylin, tocopherol, folate, Vitamin A, Vitamin C ...
Podosome rings generate forces that drive saltatory osteoclast migration. Shiqiong Hu , Emmanuelle Planus , Dan Georgess, Christophe Place , Xianghui Wang , Corinne Albiges-Rizo , Pierre Jurdic, and Jean-Christophe G minard. Mol Biol Cell. 2011 Jul 7. [Epub ahead of print] Osteoclasts are huge cells containing several nuclei formed by fusion of mononucleated cells belonging to the monocyte/macrophage lineage. They are found on the bone surface, where they remove the old bone matrix in order to allow bone rejuvenation by osteoblasts. In order to adhere to their substrates, to migrate and to resorb, they are equipped of structures called podosomes, which are loosely organized on artificial substrates (plastic or glass) but condensed on calcium apatite mineral. Podosomes are made of polymerized actin organized as dense dots, and are surrounded by a loose network of polymerized actin. They are very dynamic structures with a short life-time (2 to 3 minutes), that self organize with different patterns ...
In our investigation of the prognostic value of serum BSP in patients with primary breast cancer, we were able to show that patients with BSP values in excess of two SDs of the normal value for postmenopausal patients (,24 ng/ml) were at an enormous risk of developing skeletal metastases in the first 2-3 years after the diagnosis of disease. This is in keeping with previous immunohistochemical studies indicating an unfavorable prognosis for women with breast cancer in which the primary tumor demonstrated positive immunoreactivity for BSP (14 , 15 , 20 , 21) . The significance of the present observations, however, lies in the use of a specific and sensitive RIA that is simple to use in clinical routine and functions without the subjective assessment of immunohistochemical staining. The method also offers the possibility of carrying out follow-up determinations.. BSP, a phosphorylated glycoprotein with a molecular weight of about Mr 70,000-80,000, accounts for ∼10% of noncollagenous bone matrix ...
Lipids quantification in decellularized bovine bone.Quantification analysis of residual lipid content in bone samples DBS1, DBS2, DBS3, and DBS4 compared to NBS
The largest factory of DBM in china,stable quality of DBM in china.low price DBM in china.Appearance:white,light yellow crystalline powder.Melting point 76-80DC.Content:99%min.loss on drying 0.5% max.Ash 0.1%max.packing:25kgs.It is one new auxiliary heat stabilizer and non-toxic.it is high translucent and tasteless.it can improve the innitial tinctorial effect ability,transparency,long term stability for pvc.. ...
Fiat Chrysler is recalling over 1.3 million vehicles worldwide to fix malfunctioning alternators that can cause engines to stall or a wiring problem that can make air bags deploy unexpectedly.The wiring recall covers nearly 771,000 Dodge Journey and Fiat F
A unicameral bone cyst, also known as a simple bone cyst, is a cavity filled with a yellow-colored fluid. It is considered to be benign since it does not spread beyond the bone. Unicameral bone cysts can be classified into two categories: active and latent. An active cyst is adjacent to the epiphyseal plate and tends to grow until it fills the entire diaphysis, the shaft, of the bone; depending on the invasiveness of the cyst, it can cause a pathological fracture or even destroy the epiphyseal plate leading to the permanent shortening of the bone. A latent cyst is located away from the epiphyseal plate and is more likely to heal with treatment. It is typically diagnosed in children from the aged 5 to 15. Although unicameral bone cysts can form in any bone structure, it is predominantly found in the proximal humerus and proximal femur; additionally, it affects males twice as often as females. Most unicameral bone cysts do not cause any symptoms and are discovered as accidental findings on ...
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
A unicameral bone cyst (UBC) is a common, benign, fluid-filled lesion found almost exclusively in children. Much has been written about the diagnosis and management of these lesions, and evidence of a variety of successful treatment strategies can be found in the literature.
In this study, we showed that TGF-β signaling in prostatic fibroblasts contributed to subsequent prostate cancer bone metastasis through paracrine interactions. Conditioned medium prepared from koPFs promoted the early development and growth of prostate cancer mixed bone lesions in the tibiae of mice. This effect was, in part, mediated by elevated expression of cytokines CXCL16 and CXCL1 present in the koPFCM, which stimulated prostate cancer cell adhesion to the bone matrix and induced proliferation at an early stage.. It is well established that stromal TGF-β signaling is important for prostate tissue development as well as prostate cancer initiation and progression (9, 26-29). TGF-β is abundant in the bone matrix, and that the release of TGF-β from the matrix is a critical component for the "vicious cycle" of osteoclastic bone metastasis (12). Inhibition of TGF-β with small-molecule inhibitors or neutralizing antibodies has previously been shown to decrease osteolytic bone lesion ...
The most promising attempts to achieve bone regeneration artificially are based on the application of mediators such as bone morphogenetic proteins (BMPs) directly to the deficient tissue site. BMPs, as promoters of the regenerative process, have the ability to induce de novo bone formation in various tissues, and many animal models have demonstrated their high potential for ectopic and orthotopic bone formation. However, the biological activity of the soluble factors that promote bone formation in vivo is limited by diffusion and degradation, leading to a short half-life. Local delivery remains a problem in clinical applications. Several materials, including hydroxyapatite, tricalcium phosphate, demineralised bone matrices, poly-lactic acid homo- and heterodimers, and collagen have been tested as carriers and delivery systems for these factors in a sustained and appropriate manner. Unfortunately these delivery vehicles often have limitations in terms of biodegradability, inflammatory and immunological
Which weakens the subperiosteal resection with yellow-green. Bd_bcy adulthood, and health experts. Remains asymptomatic asymptomatic 2011... Blog.cz - Stačí otevřít a budeš v obraze.
For example, inhomogeneous deformation at the lamellae may impinge on osteocyte processes and thus trigger the osteocytes to initiate bone formation or remodelling (4.2.3). Motion at the cement lines was observed and it was suggested that such motion could act as a passive mechanism by which bones symmetry axes may become aligned to the direction of time averaged principal stresses (2.9.9). Stress on bone may induce flow of fluid in channels, e.g. canaliculi, and such flow could play a role in the nutrition and waste elimination of osteocytes, which may be significant in bone remodeling (4.2.4). In a related vein, theoretical arguments have been presented in support of the hypothesis that bone cells are directly sensitive to hydrostatic pressure transmitted to them from the bone matrix via the tissue fluid (4.2.5). Although no experimental test of this direct pressure hypothesis has been published, we observe with interest that direct hydrostatic pressure has recently been observed to alter the ...
Whole Bone Extract (Bone Matrix, Bone Marrow, Cartilage, Collagen) Pasture Raised In New Zealand Grass-fed & Grass-Finished Hormone, Pesticide & GMO Free 100% Freeze Dried Non-Defatted, Left As Organs Exist In Nature Third Party Tested For Purity Other Ingredients: Beef Gelatin Capsules, Organic Rice Concentrate, Silica To know more about the wise ways of our early ancestors, go to Ancestral Supplements (or) visit our page dedicated to all things Grass Fed Bone Marrow-related.
The North American Spine Society has opened a 30-day public comment period for the recently developed draft coverage recommendations on allograft and demineralized bone matrix...
Fiat Detroit - 28 images - Crossing The Country In Our Four Seasons 2016 Fiat 500x, Detroit 2016 Fiat 124 Spider Gtspirit, Naias 2013 Fiat Abarth Live From Detroit Autoedizione, Fiat 500 Al Salone Di Detroit 2011 10 14, Fiat 174 Freakout 2016 Rolls Into Detroit
Fiat Bravo 1.4 16V 2008|2017. Fiat Bravo 1.4 2007|2017. Fiat Bravo 1.4 LPG 2008|2014. Fiat Bravo 1.4 T-Jet 2007|2017. Fiat Bravo 1.6 D Multijet 2007|2017. Fiat Bravo 1.6 D Multijet 2008|2017. Fiat Bravo 1.9 D Multijet 2006|2017. | eBay!
Having spent the best part of the 1990s living on a drip, Fiat has embarked on a bold mission to resuscitate a bankrupt Chrysler and take over the European operations of its Detroit rival General Motors.
Fiat has officially gained full control of Chrysler after purchasing the remaining stake for over $4 billion. CNBCs Phil LeBeau reports.
Opening of the conference and Honoring of the Shortlisted Nominees of the FIAT/IFTA Archive Achievement Awards at The Promised Land 18:30 to 20:00 ...
Produse Cosmetice - Matrix. Cumpara online de pe Esteto.ro produse din categoria Produse Cosmetice marca Matrix la cele mai bune preturi. Livrare rapida prin curier.
Unicameral bone cysts (UBC), also known as simple bone cysts, are common benign non-neoplastic lucent bony lesions that are seen mainly in childhood and typically remain asymptomatic. They account for the S (simple bone cyst) in FEGNOMASHIC, the ...
Segments. The global bone graft substitute market has been segmented on the basis of type and application. Based on type, the market has been segmented as autograft, demineralized bone matrix, synthetic bone grafts, bone morphogenetic protein, and others. Based on the application, the market has been segmented as a spinal fusion, joint reconstruction, long bone, dental, foot & ankle, and others.. Browse Full Report Details @ https://www.marketresearchfuture.com/reports/bone-graft-substitutes-market. Regional analysis. The US accounts for the significant market share due to extensive use of medications and greater expenditure on health care. The fastest uptake of new technology and devices in the US is also an important driver of the global bone graft substitute market. The concentration of the major medical device manufacturers in the developed regions is also adding fuel to the market.. Europe is the second largest market due to a high disposable income and rising awareness regarding bone graft ...
A bone graft substitute composition can include essentially of calcium sulfate, a mixing solution, and a plasticizing substance. A bone graft substitute composition can include calcium sulfate, demineralized bone matrix, cancellous bone, a plasticizing substance, and a mixing solution.
OBQ08.63) A 9-year-old child presents to your office after he felt a painful snap in his arm while throwing a baseball. He is otherwise in good health and has no family history of disease. A radiograph of his arm is shown in Figure A. What is the next step in your management? Review Topic , Tested Concept ...
The majority of bone density, up to 90%, is formed by age 18 in women and age 20 in men. There are two types of cells which form and maintain healthy bone, osteoblasts are responsible for laying down healthy bone matrix which then undergoes mineralization with the aid of calcium, which adds strength. Osteoclasts are cells which are responsible for resorbing older bone, in so doing, the osteoclasts ensure that remaining bone is healthy. In the early years of our lives, the activity of osteoblasts are balanced with osteoclasts. In osteoporosis, the osteoblasts have slowed in their formation of bone, however resorption of bone has continued at its usual rate. Because of this imbalance, bone slowly thins placing a woman at risk of a break or fracture. A number of factors can influence the balance and imbalance of this process:. ...
A high concentration of PTH can promote the transformation of preosteoclasts and stromal cells into osteoclasts. Highly active osteoclasts dissolve bone minerals by secreting acid onto the isolated surface, and simultaneously break down the bone matrix by secreting collagenase and hydrolase. Increased levels of glycylproline, the end product of collagen metabolism, were found when bone collagen was disrupted. Glycylproline can be further digested by prolidase to glycine and proline, and we found decreased glycylproline and increased glycine levels in urine collected from the LCG rats. Glycine is involved in the synthesis of collagen. Phenylacetylglycine is a glycine conjugate of phenylacetic acid. Decreased glycylproline and increased glycine and phenylacetylglycine levels in urine suggested disruption of bone collagen. The results of our study suggest that glycylproline, glycine, and phenylacetylglycine could be involved in the metabolic pathway of the degradation and biosynthesis of bone ...
The Soft Tissue in Dinosaur Bone - The soft tissue in dinosaur bone would have carried blood vessels, bone matrix and osteocytes. Find out more about the soft tissue in dinosaur bone.
The new Accell Evo3 contains more of the patented Accell Bone Matrix than Integras previous generation Accell products. The optimised platform of Accell Evo3 enables the bone healing process to take advantage of the naturally available bone proteins found in DBM and ABM. Accell Evo3 is marketed in combination with a new custom designed open bore syringe.. Accell Evo3 may be used as a bone graft extender in the spine, extremities and pelvis or as bone void filler for the extremities and pelvis, and may replace the need to harvest bone graft material from the iliac crest, thus sparing the patient additional surgery and post-operative pain.. ...
The role of collagen fibres in biological bone-bonding of crystalline hydroxyapalite implant was analyzed. The mechanism involved interdigitation of collagen fibres into the surface layers of implant material. It was found that the bonding zone consists of nano-sized crystallites and organic bone matrix of collagen fibres. It was also found that the incorporation of collagen fibres with hydroxyapatite was necessary in the toughening and strengthening of bone bonding ...
Food Science. Macro- and micronutrients contribute to skeletal health by supporting bone matrix production and mineralization. Of these, calcium, vitamin D and proteins are the most important nutrients for supporting the skeleton (Heaney 1995; Bonjour 2005).. However, because there is very little boron in the soil, Americans and many people in other developed countries, suffer with osteoporosis, even though the dietary intake of calcium and vitamin D is adequate according to the recommended daily allowances (RDA).. Three dietary factors, independent of calcium and/or vitamin D, strongly influence bone and mineral homeostasis and long-term bone health. These are carbohydrate-bound boron, as found in Fructo Borate and No 7. Systemic Booster; polyphenol rich foods as found in Phyto Power and No. 7 Systemic Booster; and prebiotic and probiotic as found in No. 7 Systemic Booster. No 7 also contains supernatant (rich nutients), vitamin D3, and more (for more on nutrients, see McCabe, 2015; Sacco, ...
2016) Treatment of articular cartilage defects with microfracture and autologous matrix-induced chondrogenesis leads to extensive subchondral bone cyst formation in a sheep model. The American Journal of Sports Medicine, 44 (10). pp. 2629-2643. Smith, R.L., Murphy, D.J., Day, R.E. and Lester, G.D. ...
Many bone cysts will eventually heal without the need for treatment and wont cause long-term problems - particularly unicameral bone cysts in children, which will usually disappear by the time they stop growing.. If a bone cyst does not get better, or if treatment is recommended to help reduce the risk of a fracture, several options may be available.. For example, steroid medication can be injected into the bone to encourage the cyst to heal. If this doesnt help, surgery may be needed to drain the fluid and fill the hole with chips of bone.. There is a significant chance of the cyst recurring after treatment, so you may need to have regular X-rays for a few years afterwards to look for signs of the condition returning.. Read more about treating bone cysts.. ...
What does Bone-Up® do? BONE-UP® provides ossein hydroxyapatite complex (OHC) (from free-range Australian/New Zealand calves), which includes the superior combination of the inorganic calcium lattice of microcrystalline hydroxyapatite (MCHA) within an organic protein milieu rich in naturally occurring growth factors. The OHC is combined with vitamin D3 and vitamin K2 as MK-7 (a more bioavailable form of vitamin K) to support the deposition of calcium into the bones as well as to assist in building up the organic bone matrix.* Potassium citrate is also added for optimal osteo support. HEALTH CLAIM: Adequate calcium and vitamin D as part of a healthful diet, along with physical activity, may reduce the risk of osteoporosislater in life. For best results, use with Jarrow FORMULAS® JarroSil®, the biologically Activated Silicon®. Suggested Usage Take 3 capsules twice per day with meals, preferably dividing the number of capsules equally with each meal (for a total of 6) to facilitate
Source Naturals N-A-G, N-Acetyl Glucosamine forms the basis of complex molecular structures which are key parts of the connective tissues and mucous membranes of the body - tendons, ligaments, cartilage, bone matrix, skin, joint fluid, and intestinal lining.
Xenogenic graft of bone origin has been used to favor bone regeneration and toreduce autogenous bone needs. The purpose of this study was to evaluate thebone repair induced by particulate bovine organic bone grafted in rabbitcalvaria. Two standardized bone defects were prepared in both parietal of 20adult rabbits. The defects were filled by clot, autograft, medular organic bovinebone or cortical organic bovine bone. Thirty days following surgery animalswere killed; the calvaria were removed, fixed in formol, demineralized in EDTAand embedded in paraffin. Histological sections were stained in HE or MalloryTrichrome and analyzed by light microscope. On group filled with clot only thehalf of the defect was repaired by new bone tissue, which was formed fromlesion border, central area presented cellular connective tissue. In defects filledwith autograft almost all defect was repaired by bone tissue. Defects graftedwith medular or cortical bovine bone exhibited near ¼ of defect filled by bonetissue, ...
/PRNewswire/ -- BME, Inc. (BioMedical Enterprises, Inc., www.bme-tx.com), the leader and innovator of Nitinol shape memory bone fixation systems, announces the...
The 2012 Fiat 500 Sports comes in a high-gloss Brilliant Red, among other choices, that truly befits its flashy urban style and surprising small car performance.
High Barrier Silicon Schottky Diodes: MACOMs MSS50,000 Series of Schottky diodes are fabricated on N-Type epitaxial substrates using proprietary processes that yield the highest FCOs in the industry. Optimum mixer performance is obtained with LO power of +2 dBm to +8 dBm per diode.
The calvaria is the upper part of the skull. Its made up of four main bones, and varies in shape from person to person, with some...
The calvaria is the upper part of the skull. Its made up of four main bones, and varies in shape from person to person, with some...
How to choose an anterior matrix part 3 An article by Monaldo Saracinelli Recently, with the Styleitaliano consultation, a very well-known company has introduce...
i need to write a code that solve this issue... if the number of circulating was 4 then.... if the matrix first was : 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4
PsndCsrV writes in with a review of the new Matrix movie, below. Rottentomatoes says that the reviews overall are more favorable than un-. Ebert likes it well enough....
Multipotent adult progenitor cells are a recently described population of stem cells derived from the bone marrow stroma. multipotent adult progenitor cells along mesodermal lineages and exhibited the enhanced expression of alkaline phosphatase and production of calcium-containing mineral debris by multipotent adult progenitor cells, necessary precursors for osteogenesis. In combination with a demineralized bone matrix scaffold, multipotent adult progenitor cells exhibited enhanced revascularization and new bone formation in vivo in an orthotopic defect model when compared to mesenchymal stem cells on demineralized bone matrix or demineralized bone matrixConly control groups. The potent combination of angiogenic and osteogenic properties provided. ...
TY - JOUR. T1 - Uremia induces the osteoblast differentiation factor Cbfa1 in human blood vessels. AU - Moe, Sharon. AU - Duan, Danxia. AU - Doehle, Brian P.. AU - ONeill, Kalisha D.. AU - Chen, Xuening (Neal). PY - 2003/3/1. Y1 - 2003/3/1. N2 - Background. Bone matrix proteins are expressed in calcified arteries from dialysis patients, suggesting that vascular smooth muscle cells (VSMCs) may transform to osteoblast-like cells. One of the key transcriptional regulators of osteoblast differentiation is Cbfa1. Thus, we hypothesized that this may be a key factor in arterial calcification. Methods. To test this hypothesis, we examined sections of the inferior epigastric artery from uremic patients for the presence of Cbfa1 and type I collagen and osteopontin by in situ hybridization and immunostaining. We also examined the effect of pooled uremic sera from dialysis patients on the expression of Cbfa1 by reverse transcription-polymerase chain reaction (RT-PCR) in bovine VSMCs in vitro. Results. ...
Pharmacological Action:. Raloxifene : Raloxifene binds to estrogen receptors, resulting in differential expression of multiple estrogen-regulated genes in different tissues. Raloxifene produces estrogen-like effects on bone, reducing resorption of bone and increasing bone mineral density in postmenopausal women, thus slowing the rate of bone loss. The maintenance of bone mass by raloxifene and estrogens is, in part, through the regulation of the gene-encoding transforming growth factor-β3 (TGF-β3), which is a bone matrix protein with antiosteoclastic properties. Raloxifene activates TGF-β3 through pathways that are estrogen receptor-mediated but involve DNA sequences distinct from the estrogen response element. The drug also binds to the estrogen receptor and acts as an estrogen agonist in preosteoclastic cells, which results in the inhibtion of their proliferative capacity. This inhibition is thought to contribute to the drugs effect on bone resorption. Other mechanisms include the ...
Fujisawa R (Mar 2002). "[Recent advances in research on bone matrix proteins]". Nihon Rinsho. Japanese Journal of Clinical ... Lumachi F, Ermani M, Camozzi V, Tombolan V, Luisetto G (Sep 2009). "Changes of bone formation markers osteocalcin and bone- ... Lumachi F, Camozzi V, Tombolan V, Luisetto G (Sep 2009). "Bone mineral density, osteocalcin, and bone-specific alkaline ... or bone-building, by nature. It is also implicated in bone mineralization and calcium ion homeostasis. Osteocalcin acts as a ...
... research suggests it plays a role in anchoring osteoclasts to the mineral matrix of bones. The organic part of bone is about 20 ... OPN expression in bone predominantly occurs by osteoblasts and osteocyctes (bone-forming cells) as well as osteoclasts (bone- ... Along with its role in the regulation of normal mineralization within the extracellular matrices of bones and teeth, OPN is ... McKee MD, Addison WN, Kaartinen MT (2005). "Hierarchies of extracellular matrix and mineral organization in bone of the ...
Takeuchi Y, Kodama Y, Matsumoto T (Dec 1994). "Bone matrix decorin binds transforming growth factor-beta and enhances its ... Takeuchi Y, Kodama Y, Matsumoto T (Dec 1994). "Bone matrix decorin binds transforming growth factor-beta and enhances its ... Fujisawa R (Mar 2002). "[Recent advances in research on bone matrix proteins]". Nihon Rinsho. Japanese Journal of Clinical ... bone sialoproteins I and II, and osteonectin from the mineral compartment of developing human bone". The Journal of Biological ...
"Bone regeneration mediated by biomimetic mineralization of a nanofiber matrix". Biomaterials. 31 (23): 6004. doi:10.1016/j. ... with potential applications in bone and cartilage regeneration, angiogenesis for ischemia or peripheral artery disease, cancer ... amphiphiles with the ability to self-assemble into nanoscale filaments that mimic components of the extracellular matrix. ...
2005). "Matrix extracellular phosphoglycoprotein (MEPE) is highly expressed in osteocytes in human bone". J. Bone Miner. Metab ... MacDougall M, Simmons D, Gu TT, Dong J (2003). "MEPE/OF45, a new dentin/bone matrix protein and candidate gene for dentin ... "Entrez Gene: MEPE matrix, extracellular phosphoglycoprotein with ASARM motif (bone)". Petersen DN, Tkalcevic GT, Mansolf AL, ... Matrix extracellular phosphoglycoprotein (Osteoblast/osteocyte factor 45) is a protein that in humans is encoded by the MEPE ...
Zylberberg, L.; Laurin, M. (2011). "Analysis of fossil bone organic matrix by transmission electron microscopy". Comptes Rendus ... Bone. 46 (3): 827-834. doi:10.1016/j.bone.2009.10.035. ISSN 8756-3282. Guillerminet, F.; Fabien-Soulé, V.; Even, P. C.; Tomé, D ... bone (main component of the organic part of bone) Type II: cartilage (main collagenous component of cartilage) Type III: ... Benhamou, C.-L.; Roux, C.; Blais, A. (2012-07-01). "Hydrolyzed collagen improves bone status and prevents bone loss in ...
They received $100.000 for their Osteogenic Bone Matrix (OBM) innovation. Pires is a board member of the Licensing Executive ...
Osteopontin is involved in mineralization in the extracellular matrices of bones and teeth. ...
... is an acidic extracellular matrix glycoprotein that plays a vital role in bone mineralization, cell-matrix ... Osteonectin is also detectable in osteoid, bone matrix proper, and dentin. Osteonectin has been localized in a variety of ... Osteonectin is a glycoprotein in the bone that binds calcium. It is secreted by osteoblasts during bone formation, initiating ... and regulation of the major noncollagenous matrix proteins of bone". Clinical Orthopaedics and Related Research (281): 275-94. ...
Sulfatases are essential for the correct composition of bone and cartilage matrix. Deficiencies in ARSE are associated with X- ... linked recessive chondrodysplasia punctata, a disease characterized by abnormalities in cartilage and bone development. GRCh38 ...
Extensive bone remodeling has occurred in the internal matrix of the rib. The medullary cavity, which houses the marrow, is ... On the outer surface of the bone, osteons have almost completely replaced the interior of the bone; the layered growth of the ... The supraoccipital bone, which forms the top portion of the back of the skull, is flat and nearly vertical, as is the case in ... Among the metacarpal bones of the hand, the second is curved, the third is straight, and the fourth is strongly curved. Like ...
Medical science now uses corallines in the preparation of dental bone implants. The cell fusions provide the matrix for the ... regeneration of bone tissue. Since coralline algae contain calcium carbonate, they fossilize fairly well. They are particularly ...
Takeuchi Y, Kodama Y, Matsumoto T (Dec 1994). "Bone matrix decorin binds transforming growth factor-beta and enhances its ...
Enamel Matrix Protein These agents not only help improve wound healing but also stimulate regeneration of the lost bone, ... Rathe, F; Junker, R; Chesnutt, B. M.; Jansen, J. A. (2009). "The effect of enamel matrix derivative (Emdogain) on bone ... PGE2 upregulates bone resorption by osteoclasts and their levels are higher in patients with periodontal disease than in ... Growth Factors Bone Morphogenic Proteins Michael G. Newman; Henry Takei; Perry R. Klokkevold; Fermin A. Carranza (14 February ...
... is a bone-like matrix that is porous and yellow-hued material. It is made up of 70% inorganic materials (mainly ... Matrix metalloproteinases (MMPs) and their specific tissue inhibitors (TIMPs) in mature human odontoblasts and pulp tissue. ... It is similar to osteoid in bone and is thickest when dentinogenesis is occurring. Secondary dentin is formed after root ... Cells and Extracellular Matrices of Dentin and Pulp: A Biological Basis For Repair and Tissue Engineering, M. Goldberg and A.J ...
2004). "Dentin matrix protein 1 is expressed in human lung cancer". J. Bone Miner. Res. 18 (8): 1506-12. doi:10.1359/jbmr. ... 2004). "Bone morphogenetic protein-1/Tolloid-like proteinases process dentin matrix protein-1". J. Biol. Chem. 279 (2): 980-6. ... 2006). "A chondroitin sulfate chain attached to the bone dentin matrix protein 1 NH2-terminal fragment". J. Biol. Chem. 281 (12 ... Dentin matrix acidic phosphoprotein 1 is a protein that in humans is encoded by the DMP1 gene. Dentin matrix acidic ...
This sealing zone becomes the site of osteoclast attachment to the bone matrix. Inhibition of bone resorption through drug ... Osteoclasts are large, multinucleated bone cells that conduct the process of bone resorption. In this remodeling process, ... Linder, S (March 2007). "The matrix corroded: podosomes and invadopodia in extracellular matrix degradation". Trends in Cell ... Upon initiation of bone resorption, the band of podosomes disassembles leaving behind a mesh primarily composed of F-actin ...
This matrix can be liquid or rigid. For example, blood contains plasma as its matrix and bone's matrix is rigid. Connective ... Blood, bone, tendon, ligament, adipose and areolar tissues are examples of connective tissues. One method of classifying ... A tissue is an ensemble of similar cells and their extracellular matrix from the same origin that together carry out a specific ... Mineralized tissues are biological tissues that incorporate minerals into soft matrices. Such tissues may be found in both ...
Flexible, bifurcating blood vessels and fibrous but elastic bone matrix tissue were recognized. In addition, microstructures ... Bucky also has a nearly complete set of gastralia, or belly ribs, and an ulna, or lower arm bone. As of now, 101 bones, or ... A piece of bone is missing at the rear, and the skull also bears a hole 1 inch wide, probably made by another Tyrannosaurus. ... A large bone was found in the riverbank and shown to their teacher. Soon afterward, the Royal Tyrrell Museum was contacted, and ...
... homeobox genes and extracellular matrix". Matrix Biol. 14 (8): 599-606. doi:10.1016/S0945-053X(05)80024-1. PMID 9057810. ... GDF6 interacts with bone morphogenetic proteins (BMPs) to form heterodimers that may work to regulate neural induction and ... GDF6 has been shown to play an important role in the patterning of the epidermis and bone and joint formation. GDF6 induces ... 2009). "BMP-13 Emerges as a Potential Inhibitor of Bone Formation". Int. J. Biol. Sci. 5 (2): 192-200. doi:10.7150/ijbs.5.192. ...
"Enhanced activity of demineralised bone matrix augmented with xenogeneic bone morphogenetic protein complex in rats". SADJ. 67 ... Enhanced activity of demineralised bone matrix augmented with xenogeneic bone morphogenetic protein complex in rats. (2012 ... bone morphogenetic protein-7) to initiate rapid bone formation". J Bone Miner Res. 12: 1584-95. doi:10.1359/jbmr.1997.12. ... Together with Nuno Pires he won the prize for the Altis Osteogenic Bone Matrix. Duneas and Pires received USD 100.000. Limited ...
... but no matrix. The osteoclasts consume the trabecular bone that osteoblasts lay down and this front of reparative bone ... Hemosiderin deposition is not a distinctive feature of brown tumors; it may also be seen in giant cell tumors of the bone. ... The brown tumor is a bone lesion that arises in settings of excess osteoclast activity, such as hyperparathyroidism. It is not ... It most commonly affects the maxilla and mandible, though any bone may be affected. Brown tumours are radiolucent on x-ray. ...
... homeobox genes and extracellular matrix". Matrix Biol. 14 (8): 599-606. doi:10.1016/S0945-053X(05)80024-1. PMID 9057810. Luyten ... The protein encoded by this gene is closely related to the bone morphogenetic protein (BMP) family and is a member of the TGF- ... Ducy P, Karsenty G (2000). "The family of bone morphogenetic proteins". Kidney Int. 57 (6): 2207-14. doi:10.1046/j.1523- ... New members of the transforming growth factor-beta superfamily predominantly expressed in long bones during human embryonic ...
Fujisawa R (2002). „[Recent advances in research on bone matrix proteins]". Nippon Rinsho. 60 Suppl 3: 72-8. PMID 11979972.. ... 2009). „Changes of bone formation markers osteocalcin and bone-specific alkaline phosphatase in postmenopausal women with ... Lumachi F, Camozzi V, Tombolan V, Luisetto G (2009). „Bone mineral density, osteocalcin, and bone-specific alkaline phosphatase ... "J. Bone Miner. Res. 24 (12): 2039-49. PMC 2791518 . PMID 19453261. doi:10.1359/jbmr.090524.. CS1 одржавање: Експлицитна ...
"The enhancement of bone regeneration by gene activated matrix encoding for platelet derived growth factor". Biomaterials. 35 (2 ... In experiments, it new bone fully covered skull wounds in test animals and stimulated growth in human bone marrow stromal cells ... A non-viral PDGF "bio patch" can regenerate missing or damaged bone by delivering DNA in a nano-sized particle directly into ... Repairing bone fractures, fixing craniofacial defects and improving dental implants are among potential uses. The patch employs ...
Coluzzi F, Mandatori I, Mattia C (September 2011). "Emerging therapies in metastatic bone pain". Expert Opin Emerg Drugs. 16 (3 ... In particular, abnormal interactions between epithelial cells and the extracellular matrix are associated with the over- ...
bone matrix synonyms, bone matrix pronunciation, bone matrix translation, English dictionary definition of bone matrix. n. A ... structural unit of bone consisting of a haversian canal and corresponding lamellae of compact bone. Also called haversian ... Bone matrix - definition of bone matrix by The Free Dictionary https://www.thefreedictionary.com/bone+matrix ... demineralized bone matrix allografts, and bone graft substitutes - bone morphogenic proteins and synthetic bone graft extenders ...
The matrix is manufactured from protein-extracted bone powder treated with certain swelling agents to increase its surface area ... The matrix may be combined with osteogenic protein to induce reliably and reproducibly endochondral bone formation. It also can ... Disclosed is a matrix material for implantation in a mammalian host comprising biocompatible mineral-free type I bone collagen ... in rat bone matrix over that of bovine bone matrix. It has been discovered that increasing porosity helps entrap protein, and ...
Demineralized bone matrix (DBM) is allograft bone that has had the inorganic mineral removed, leaving behind the organic " ... It was first discovered by Marshall Urist in 1965 that the removal of the bone mineral exposes more biologically active bone ... Graham, S.M., et al., Biological therapy of bone defects: the immunology of bone allo-transplantation. Expert Opinion on ... The success of a bone graft is determined by its ability to recruit host cells to the site of the graft and modulate their ...
... Yoshinobu Uchihara,1 Manabu Akahane,2 Takamasa ... C. Maniatopoulos, J. Sodek, and A. H. Melcher, "Bone formation in vitro by stromal cells obtained from bone marrow of young ... "Osteogenic matrix sheet-cell transplantation using osteoblastic cell sheet resulted in bone formation without scaffold at an ... "Bone marrow-derived mesenchymal cells can rescue osteogenic capacity of devitalized autologous bone," Journal of Tissue ...
... Andreas O. Parashis,1,2 Charalampos J. ... and histological outcomes following use of a bilayer xenogeneic collagen matrix (XCM) in combination with freeze-dried bone ... The XCM was adapted to cover the defect and 2-3 mm of adjacent bone and flaps were repositioned. Healing was uneventful in all ... Exposed matrix portions were slowly vascularized and replaced by mature keratinized tissue within 2-3 months. Radiographic and ...
Make sure you apply the bones offset matrix to the bone before you generate a bounding area around it. Then apply the bones ... The offset matrix should orientate the bone so that it is straight.. After that, just apply each bones final transform matrix ... m having is with the matrix math to orient that geometry at the position of the bones orientation. Having a few matrices to ... Matrix Math - Bone Hierachy Orientation By Carandiru, June 5, 2004. in Math and Physics ...
... containing protease inhibitors to solubilize putative inductive molecules in the bone matrix. Extraction of bone matrix with ... HCl-treated bone matrix and then implanted, new bone was induced. These observations demonstrate the dissociative extraction ... The various reconstituted matrices were bioassayed for bone inductive potential by quantitation of alkaline phosphatase ... Dissociative extraction and reconstitution of extracellular matrix components involved in local bone differentiation. T K ...
... similar to what we find in natural bone --while the bone matrix formed by the static samples was thin and brittle." Mikos said ... bone cells called "osteoblasts" and several growth factors that osteoblasts use to build the mineralized matrix of bones -- but ... This photograph from an electron microscope shows a pore that has formed in a 3-D bony matrix. Bone-forming cells are clearly ... Rice bioengineers develop method to grow 3-D bone matrix. 04.10.2002 ...
I believe all Im looking for is the bind pose matrix for each individual bone in my armature. There are a few options in the ... armature[x].bone[x].matrix. armature[x].bone[x].matrix_local. and in the posebone. bone.matrix. bone.matrix_basis. bone.matrix_ ... Exporting bone bind pose matrices Hello all,. Its my first time using these forums, so please correct me if Im putting this ... I believe the matrix in question Im looking for is 4x4, and should be connected to the bone data in the armature. Ive tried ...
FUENTES, Ramón et al. The Behavior of Demineralized Bone Matrix (DBM) in Post-Extraction Sockets. Int. J. Morphol. [online]. ... Autogenous bone grafts are considered to be the gold standard in bone regeneration because of their osteogenic activity; ... A very mature bone was observed at 6 months after DBM graft placement in the sockets, showing it to be a good alternative as ...
... is designed to be injected into damaged bone, which then harde ... Injectable Matrix to Help Regrow Damaged Bones. December 8th, ... is designed to be injected into damaged bone, which then hardens into a strong scaffold as a medium for new bone tissue to grow ... In more challenging applications, stem cells, matrix and/or growth factors must be provided within the 3D template. The ... When a tissue develops or repairs itself a "regenerative niche" is formed comprising cells, 3D template, extracellular matrix ...
In the second application we mapped the calcium content in a human bone matrix in close proximity to osteocyte lacunae ( ... perilacunar matrix). A concurrently acquired ptychographic image was used to remove the mass-thickness effect from the raw ... calcium distribution allowed then to observe a locally lower degree of mineralization of the perilacunar matrix. ... Bone Matrix Mineralization. Pathologies of the skeleton are among the most common morbidities worldwide. Age-related bone loss ...
2 bones in total) in global coordinates. Ok. these points p1, p2, p3 are drawn in global coordinates (see values X,Y). Now I ... Matrix mx_parent_global = pnt[i-1].globalMatrix; // parent joint global matrix // Now I need to work out the local matrix for ... Global to local transformation - matrices, bones GameDev.net needs your input - and you could win a $25 Amazon gift card! Click ... Global to local transformation - matrices, bones By papa, September 21, 2004. in Math and Physics ...
... announced the release of its CONFORM SHEET demineralized cancellous bone scaffold thats designed for implantation during ... CONFORM SHEET Bone Matrix for Spinal Fusions Now in U.S.. January 6th, 2014 Medgadget Editors Neurosurgery, Orthopedic Surgery ... When combined with bone marrow aspirate, CONFORM SHEET becomes osteogenic.. "CONFORM SHEET has excellent handling ... It is compressible for precise placement, where it can then expand to fill the bone void," said Khalid Sethi, MD, FACS, Chief ...
J Bone Miner Res. 1999 Nov;14(11):1902-8. Research Support, Non-U.S. Govt; Research Support, U.S. Govt, P.H.S. ... Determination of bone markers in pycnodysostosis: effects of cathepsin K deficiency on bone matrix degradation.. Nishi Y1, ... of organic bone matrix. To determine the in vivo effects of cathepsin K mutations on bone metabolism in general and osteoclast- ... and is secreted into the subosteoclastic space where bone matrix is degraded. In vitro studies revealed that mutant cathepsin K ...
... pose.bones["leg1.R"] armaturematrix = Matrix(Object.Get("Armature").matrixWorld) ws_bindposematrix = Matrix(bone.matrix[" ... a bones world space bind pose matrix. - a bones world space animation frame matrix. i think i know how to calculate ... pose_mat = Matrix(pose_bone.poseMatrix). pose_mat.invert(). to avoid inverting the matrix. (This behavior is consistent with ... This is the bone transformation trick; theyre hierarchical so each bone(b). is in the coord system of bone(b-1):. arm_mat(b)= ...
Immediate loading may be preferable in order to ensure robust bone formation in vivo. The use of a secondary ECM carrier ... with or without an extracellular matrix-based seeding suspension, on cell engraftment and osteogenesis. When quantitatively ... Cell-based tissue engineering can be used to replace missing or damaged bone, but the optimal methods for delivering ...
Using a rat ectopic bone formation model, we have injected rhBMP-2 into a collagen matrix with or without a bifunctional BMP-2 ... and bone maturity at the site of injection. Our results suggest that bifunctional peptides that can simultaneously bind to a ... collagen peptide (BC-1). The presence of BC-1 significantly increased osteogenic cellular activity, the area of bone formed, ... tissue engineering approaches are being developed that combine growth factors such as Bone Morphogenetic Proteins (BMP) with ...
... noncollagenous proteins of the organic matrix of chicken bone by Sephadex G-100 molecular sieving has revealed that the ... γ-carboxyglutamic acid-containing peptide of bone matrix.. Key words. Phosphoprotein Bone 31P-NMR Phosphoserine ... Lee, S. L., Glimcher, M. J.: Bone matrix phosphoproteins from adult avian metatarsals, J. Cell Biol.83:464a, 1979Google Scholar ... Fractionation of the EDTA-soluble, noncollagenous proteins of the organic matrix of chicken bone by Sephadex G-100 molecular ...
... extracellular matrix protein binding, extracellular matrix structural constituent, negative regulation of bone mineralization, ... Matrix extracellular phosphoglycoprotein with ASARM motif (bone)Imported. ,p>Information which has been imported from another ... tr,Q8K4L6,Q8K4L6_MOUSE Matrix extracellular phosphoglycoprotein with ASARM motif (bone) OS=Mus musculus GN=Mepe PE=2 SV=1 ... negative regulation of bone mineralization Source: MGI ,p>Inferred from Direct Assay,/p> ,p>Used to indicate a direct assay for ...
Home , October 2011 - Volume - Issue , REDUCED DOSE OF RHBMP‐2 WITH DEMINERALIZED BONE MATRIX‐BASED... ... REDUCED DOSE OF RHBMP‐2 WITH DEMINERALIZED BONE MATRIX‐BASED PRODUCT FOR SPINAL FUSION: GP101.. Delamarter, Rick MD; Kropf, ... REDUCED DOSE OF RHBMP‐2 WITH DEMINERALIZED BONE MATRIX‐BASED PRODUCT FOR SPINAL FUSION: GP101. ... As is clinically practice, autogeneous bone added to the DBM‐based product also enhanced rapid bone formation. ...
... vivo was successfully fabricated by simple subcutaneous injection of chitosan and GP solutions with demineralized bone matrix ( ... we developed an injectable carrier for demineralized bone matrix (DBM) which consists of chitosan and glycerol phosphate (GP). ... In this study, we developed an injectable carrier for demineralized bone matrix (DBM) which consists of chitosan and glycerol ... Y. H. Kim et al., "Injectable Chitosan Carrier for Demineralized Bone Matrix", Key Engineering Materials, Vols. 342-343, pp. ...
What is bone matrix? Meaning of bone matrix as a finance term. What does bone matrix mean in finance? ... Definition of bone matrix in the Financial Dictionary - by Free online English dictionary and encyclopedia. ... Bone matrix financial definition of bone matrix https://financial-dictionary.thefreedictionary.com/bone+matrix ... matrix. (redirected from bone matrix). Also found in: Dictionary, Thesaurus, Medical, Legal, Encyclopedia. Fig. 58 Matrix. The ...
The contribution of the mineral phase to bones mechanical properties has dominated scientific thinking. Collagens role has ... Bone is a two-phase porous composite material comprised primarily of collagen and mineral, which together provide its ... The Contribution of the Organic Matrix to Bones Material Properties Bone. 2002 Jul;31(1):8-11. doi: 10.1016/s8756-3282(02) ... it may have a profound effect on bone fragility. Collagen changes that occur with age and reduce bones toughness may be an ...
  • Alveolar bone reconstruction with a DFDBM allograft mixed with calcium sulphate graft binder was performed immediately. (jcda.ca)
  • Posterior mandibular extraction sites with compromised buccal alveolar bone may be effectively managed by immediate alveolar augmentation using a mixture of DFDBM allograft and calcium sulphate graft binder. (jcda.ca)
  • A question of interest to general practitioners and specialists working in endosseous implantology is how to approach reconstruction of a postextraction alveolar bone defect. (jcda.ca)
  • Pre-Operative Background Pre-operative clinical radiographic exam revealed adequate alveolar bone height in the #12 and #13 sites, however, the bone height was inadequate in the #14 and #15 sites due to the pneumatized sinus (Fig 1). (ddsgadget.com)
  • Periodontitis involves progressive loss of the alveolar bone around the teeth, and if left untreated, can lead to the loosening and subsequent loss of teeth. (wikipedia.org)
  • Specifically, in aim 1 we will address the working hypothesis that in the context of early osteoblast-differentiation events, TSP2 promotes the assembly of a type I collagen-rich osteoblast-derived extracellular matrix, and as a result, it indirectly promotes collagen-dependent signal transduction events that are critical for osteoblast differentiation. (grantome.com)
  • The cell lines were cultured atop radiolabelled matrices of either mineralized or non-mineralized bone or type I collagen, the principal organic constituent of bone. (biomedcentral.com)
  • We studied in vitro the capacity of MSC to migrate through bone marrow endothelium. (haematologica.org)
  • C. Knabe, A. Houshmand, G. Berger, P. Ducheyne , R. Gildenhaar , I, Kranz , M. Stiller, Effect of rapidly resorbable bone substitute materials on the temporal expression of the osteoblastic phenotype in vitro, J. Biomed Mater Res A. 84 (2008). (scientific.net)
  • Therefore, the aim of this study was to investigate the effect of IFN-γ on the immunomodulatory capacity of C-MSC in vitro and to test the bone regenerative activity of C-MSC or IFN-γ-pretreated C-MSC (C-MSCγ) xenografts in a mice calvarial defect model. (biomedcentral.com)
  • In contrast, the highly undifferentiated, bone-derived human prostate cancer cell line PC3 did not produce an osteoblastic reaction in vitro and induced osteolytic lesions in vivo . (aacrjournals.org)
  • Indeed, no reliable in vitro model of osteoblastic bone metastasis from prostate cancer is currently available. (aacrjournals.org)
  • abstract = "Mouse bone matrix powder was implanted subcutaneously or put into folded tubular cellulose ester membranes (CEM) and implanted i.p. (elsevier.com)
  • If so, should an autogenic, allogeneic, xenogeneic or synthetic bone graft material, or a combination of these products, be used for the reconstruction? (jcda.ca)
  • CONFORM SHEET Bone Matrix for Spinal Fusions Now in U.S. (medgadget.com)
  • The implant may be used with DePuy Synthes Spine MATRIX™ MIS Pedicle Screw System and EXPEDIUM® Spine System, as well as other systems for spinal fusion. (medgadget.com)
  • The Spinal Research Foundation was part of a clinical trial that examined the effect of using Trinity Evolution bone matrix as part of the surgical treatment for cervical spine issues. (spinerf.org)
  • My experience early on has shown that ViviGen has desirable handling characteristics and is an appropriate alternative to taking the patient's own bone for many of my spinal procedures," says Kennedy Yalamanchili of Christiana Care Hospital in Newark, USA, which is one of several sites that has recently used ViviGen. (spinalnewsinternational.com)
  • Bone graft refers to the use of bone in spinal fusion surgery. (spineuniverse.com)
  • The purpose of a spinal fusion (arthrodesis) procedure is to link or weld bones together. (spineuniverse.com)
  • There is a lot of interest in developing and refining bone graft substitute options for use in lumbar spinal fusion surgery procedures in order to eliminate the need to harvest the patient's own bone, thus potentially reducing the risk and pain associated with the procedure and resulting in higher fusion rates. (spine-health.com)
  • Its mouldable and firm texture is especially suitable for the filling of spinal fusion cages or bone defects that require longer-term support and volume stability. (spinalsurgerynews.com)
  • Although rhBMP-2 and rhBMP-7 are used in the treatment of a variety of bone-related conditions including spinal fusions and nonunions, the risks of this off-label treatment are not understood. (wikipedia.org)
  • There is "little debate or controversy" about the effectiveness of rhBMP-2 to grow bone to achieve spinal fusions, and Medtronic generates $700 million in annual sales from their product. (wikipedia.org)
  • Chemical changes that occur within the spinal cord as a result of bone destruction give further insight into the mechanism of bone pain. (wikipedia.org)
  • We investigated the anti-osteoarthritic effects of deer bone extract on the gene expressions of matrix metalloproteinases (MMPs) and collagen type II (COL2) in interleukin-1β-induced osteoarthritis (OA) chondrocytes. (bvsalud.org)
  • An understanding of ECM structure and composition also helps in comprehending the complex dynamics of tumor invasion and metastasis in cancer biology as metastasis often involves the destruction of extracellular matrix by enzymes such as serine proteases, threonine proteases, and matrix metalloproteinases. (wikipedia.org)
  • A rapid rat model of Mycobacterium tuberculosis monoarticular arthritis with induces of inflammation, as well as patellar cartilage proteoglycan and bone degradation has been reported. (brillonline.com)
  • We concluded that deer bone extract induces accumulation of COL2 through the down-regulation of MMPs in IL-1β-induced OA chondrocytes. (bvsalud.org)
  • Cellular matrix. (hku.hk)
  • Cellular allografts represent an exciting and fast growing new category within the bone graft substitute market," comments Max Reinhardt, president of DePuy Synthes Spine. (spinalnewsinternational.com)
  • The objective of the 5 year career development plan outlined in this KO1 application is to foster the development of the candidate, Dr. Alford into a productive, independent investigator in the field of osteoblast cellular and extracellular matrix biology. (grantome.com)
  • The transformation from motile osteoblast to entrapped osteocyte takes about three days, and during this time, the cell produces a volume of extracellular matrix three times its own cellular volume, which results in 70% volume reduction in the mature osteocyte cell body compared to the original osteoblast volume. (wikipedia.org)
  • Likewise, in age-related osteoporosis in humans, we found that bone marrow IGF-1 concentrations were 40% lower in individuals with osteoporosis than in individuals without osteoporosis. (nih.gov)
  • Together, these results provide mechanistic insight into how IGF-1 maintains adult bone mass, while also providing a further rationale for its therapeutic targeting to treat age-related osteoporosis. (nih.gov)
  • Bone: modulation of IGF-1 might prevent osteoporosis. (nih.gov)
  • Most cases of osteoporosis aren't detected until you've already 'had a fall' and ended up in hospital, only to be told that osteoporosis has already stolen around 30% of your bone. (nutriplusdirect.com)
  • Novel small peptide that has the ability to augment bone matrix production, potentiating its use for the treatment of osteoporosis and other bone related diseases. (technologypublisher.com)
  • Treatment of osteoporosis and other degenerative bone diseases. (technologypublisher.com)
  • A number of diseases can cause bone pain, including the following: Endocrine, such as hyperparathyroidism, osteoporosis, renal failure. (wikipedia.org)
  • Osteopetrosis, literally "stone bone", also known as marble bone disease, albers schonberg disease is an extremely rare inherited disorder whereby the bones harden, becoming denser, in contrast to more prevalent conditions like osteoporosis, in which the bones become less dense and more brittle, or osteomalacia, in which the bones soften. (wikipedia.org)
  • Knock-out mice that have had the gene for biglycan suppressed (Bgn -/-) have an osteoporosis-like phenotype with reduced growth rate and lower bone mass than mice that can express biglycan. (wikipedia.org)
  • Within their limits, the obtained results indicate that the combination of enamel matrix derivative and autogenous bone graft may result in non-significant additional clinical improvements in terms of CALgain, PDred, and RECinc compared with those obtained with EMD alone. (mdpi.com)
  • Particulated autogenous bone was used as a control group. (nih.gov)
  • 5 , - 9 Nowadays, a large number of filling materials are available, among which autogenous bone is still considered to be the gold standard. (allenpress.com)
  • However, harvesting autogenous bone has its disadvantages: secondary donor site surgery, extended operating time, risk of complications, as well as limited amount of graft material. (allenpress.com)
  • Autologous (or autogenous) bone grafting involves utilizing bone obtained from the same individual receiving the graft. (wikipedia.org)
  • When a block graft will be performed, autogenous bone is the most preferred because there is less risk of the graft rejection because the graft originated from the patient's own body. (wikipedia.org)
  • Although the effects of osteolytic metastasis on bone quantity have been well studied, whether osteoclast activation induced by cancer bone metastasis affects the bone microstructure, a notable aspect of the bone quality, remains uncertain. (go.jp)
  • The aim of this study was to clarify the effect of osteolytic bone metastasis in breast cancer on the microstructure of the bone matrix, particularly the integrity of collagen fibril orientation. (go.jp)
  • Oncologic, such as bone metastasis and leukemia. (wikipedia.org)
  • It is thought that this is the primary source of the dull, chronic pain experienced by patients with bone metastasis. (wikipedia.org)
  • Bone is the third most common location for metastasis, after the lung and liver. (wikipedia.org)
  • Particularly in prostate cancer, bone metastases tend to be the only site of metastasis. (wikipedia.org)
  • Even sclerotic bone metastases are generally less radiodense than enostoses, and it has been suggested that bone metastasis should be the favored diagnosis between the two for bone lesions lower than a cutoff of 1060 Hounsfield units (HU). (wikipedia.org)
  • High-intensity focused ultrasound (HIFU) has CE approval for palliative care for bone metastasis, though treatments are still in investigatory phases as more information is needed to study effectiveness in order to obtain full approval in countries such as the USA. (wikipedia.org)
  • The role of matrix metalloproteases and TIMPs in several pathological conditions including angiogenesis, tumor growth, and metastasis has been investigated and very well described. (wikipedia.org)
  • Cathepsin K, among other cathepsins, plays a role in cancer metastasis through the degradation of the extracellular matrix. (wikipedia.org)
  • The use of bone materials to promote bone healing after facture, bone loss, infection, tumor, or other pathologic conditions is well known to those skilled in the art. (justia.com)
  • Given the success of bisphosphonates in the clinic and other agents that modulate the bone microenvironment including denosumab (an inhibitor of the osteoclastogenic factor receptor activator of nuclear κB ligand-RANKL) there is strong rationale for the further development of therapeutics that limit tumor-bone interaction [ 9 ]. (oncotarget.com)
  • The brown tumor is a bone lesion that arises in settings of excess osteoclast activity, such as hyperparathyroidism. (wikipedia.org)
  • The lesion may arise de novo or may arise secondarily within a pre-existing bone tumor, because the abnormal bone causes changes in hemodynamics. (wikipedia.org)
  • An aneurysmal bone cyst can arise from a pre-existing chondroblastoma, a chondromyxoid fibroma, an osteoblastoma, a giant cell tumor, or fibrous dysplasia. (wikipedia.org)
  • Larger lesions may appear septated Following conditions are excluded before diagnosis can be confirmed: Unicameral bone cyst Giant cell tumor Telangiectatic osteosarcoma Secondary aneurysmal bone cyst Curettage is performed on some patients, and is sufficient for inactive lesions. (wikipedia.org)
  • High magnification micrograph of an aneurysmal bone cyst Intermediate magnification micrograph of an aneurysmal bone cyst Simple bone cyst (SBC) Giant cell tumor of bone Traumatic bone cyst Maroldi, Roberto (2005). (wikipedia.org)
  • Bone metastases, or osseous metastatic disease, is a category of cancer metastases that results from primary tumor invasion to bone. (wikipedia.org)
  • The largest bone in the body is the femur or thigh-bone, and the smallest is the stapes in the middle ear. (wikipedia.org)
  • Commonly affected sites are metaphyses of vertebra, flat bones, femur and tibia. (wikipedia.org)
  • Approximate percentages by sites are as shown: Skull and mandible (4%) Spine (16%) Clavicle and ribs (5%) Upper extremity (21%) Pelvis and sacrum (12%) Femur (13%) Lower leg (24%) Foot (3%) Aneurysmal bone cyst has been widely regarded a reactive process of uncertain cause since its initial description by Jaffe and Lichtenstein in 1942. (wikipedia.org)
  • This bone was later identified as part of a giant femur from a Cretaceous sauropod that roamed the Winton area 95 million years ago. (wikipedia.org)
  • The hindlimbs were relatively short, and the thigh bone (femur) was longer than the shin bone (tibia), as is common for large animals. (wikipedia.org)
  • Vascularised or non-vascularised autologous fibular grafting for the reconstruction of a diaphyseal bone defect after resection of a musculoskeletal tumour," The Bone & Joint Journal , vol. 96, no. 9, pp. 1258-1263, 2014. (hindawi.com)
  • The XCM was adapted to cover the defect and 2-3 mm of adjacent bone and flaps were repositioned. (hindawi.com)
  • The studies will provide fundamental insights, from a dynamic perspective, into the mechanisms of assembly of bone ECM and how the ECM regulates osteoblast function. (grantome.com)
  • In this model, the bone-derived prostate cancer cell lines MDA PCa 2a and MDA PCa 2b induced a specific and reproducible increase in osteoblast proliferation. (aacrjournals.org)
  • 1990) distinguish three cell types from osteoblast to mature osteocyte: type I preosteocyte (osteoblastic osteocyte), type II preosteocyte (osteoid osteocyte), and type III preosteocyte (partially surrounded by mineral matrix). (wikipedia.org)