Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.
The transference of BONE MARROW from one human or animal to another for a variety of purposes including HEMATOPOIETIC STEM CELL TRANSPLANTATION or MESENCHYMAL STEM CELL TRANSPLANTATION.
A specialized CONNECTIVE TISSUE that is the main constituent of the SKELETON. The principle cellular component of bone is comprised of OSTEOBLASTS; OSTEOCYTES; and OSTEOCLASTS, while FIBRILLAR COLLAGENS and hydroxyapatite crystals form the BONE MATRIX.
Progenitor cells from which all blood cells derive.
A cytologic technique for measuring the functional capacity of stem cells by assaying their activity.
The development and formation of various types of BLOOD CELLS. Hematopoiesis can take place in the BONE MARROW (medullary) or outside the bone marrow (HEMATOPOIESIS, EXTRAMEDULLARY).
The continuous turnover of BONE MATRIX and mineral that involves first an increase in BONE RESORPTION (osteoclastic activity) and later, reactive BONE FORMATION (osteoblastic activity). The process of bone remodeling takes place in the adult skeleton at discrete foci. The process ensures the mechanical integrity of the skeleton throughout life and plays an important role in calcium HOMEOSTASIS. An imbalance in the regulation of bone remodeling's two contrasting events, bone resorption and bone formation, results in many of the metabolic bone diseases, such as OSTEOPOROSIS.
Removal of bone marrow and evaluation of its histologic picture.
Neoplasms located in the bone marrow. They are differentiated from neoplasms composed of bone marrow cells, such as MULTIPLE MYELOMA. Most bone marrow neoplasms are metastatic.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
An organism whose body contains cell populations of different genotypes as a result of the TRANSPLANTATION of donor cells after sufficient ionizing radiation to destroy the mature recipient's cells which would otherwise reject the donor cells.
Bone loss due to osteoclastic activity.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
The amount of mineral per square centimeter of BONE. This is the definition used in clinical practice. Actual bone density would be expressed in grams per milliliter. It is most frequently measured by X-RAY ABSORPTIOMETRY or TOMOGRAPHY, X RAY COMPUTED. Bone density is an important predictor for OSTEOPOROSIS.
Leukocytes with abundant granules in the cytoplasm. They are divided into three groups according to the staining properties of the granules: neutrophilic, eosinophilic, and basophilic. Mature granulocytes are the NEUTROPHILS; EOSINOPHILS; and BASOPHILS.
Techniques for the removal of subpopulations of cells (usually residual tumor cells) from the bone marrow ex vivo before it is infused. The purging is achieved by a variety of agents including pharmacologic agents, biophysical agents (laser photoirradiation or radioisotopes) and immunologic agents. Bone marrow purging is used in both autologous and allogeneic BONE MARROW TRANSPLANTATION.
Transplantation between individuals of the same species. Usually refers to genetically disparate individuals in contradistinction to isogeneic transplantation for genetically identical individuals.
An encapsulated lymphatic organ through which venous blood filters.
Irradiation of the whole body with ionizing or non-ionizing radiation. It is applicable to humans or animals but not to microorganisms.
Glycoproteins found on immature hematopoietic cells and endothelial cells. They are the only molecules to date whose expression within the blood system is restricted to a small number of progenitor cells in the bone marrow.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
A large multinuclear cell associated with the BONE RESORPTION. An odontoclast, also called cementoclast, is cytomorphologically the same as an osteoclast and is involved in CEMENTUM resorption.
Transplantation of an individual's own tissue from one site to another site.
Glycoproteins found in a subfraction of normal mammalian plasma and urine. They stimulate the proliferation of bone marrow cells in agar cultures and the formation of colonies of granulocytes and/or macrophages. The factors include INTERLEUKIN-3; (IL-3); GRANULOCYTE COLONY-STIMULATING FACTOR; (G-CSF); MACROPHAGE COLONY-STIMULATING FACTOR; (M-CSF); and GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR; (GM-CSF).
Tumors or cancer located in bone tissue or specific BONES.
A progressive, malignant disease of the blood-forming organs, characterized by distorted proliferation and development of leukocytes and their precursors in the blood and bone marrow. Leukemias were originally termed acute or chronic based on life expectancy but now are classified according to cellular maturity. Acute leukemias consist of predominately immature cells; chronic leukemias are composed of more mature cells. (From The Merck Manual, 2006)
The growth and development of bones from fetus to adult. It includes two principal mechanisms of bone growth: growth in length of long bones at the epiphyseal cartilages and growth in thickness by depositing new bone (OSTEOGENESIS) with the actions of OSTEOBLASTS and OSTEOCLASTS.
Connective tissue cells of an organ found in the loose connective tissue. These are most often associated with the uterine mucosa and the ovary as well as the hematopoietic system and elsewhere.
Very large BONE MARROW CELLS which release mature BLOOD PLATELETS.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
An organism that, as a result of transplantation of donor tissue or cells, consists of two or more cell lines descended from at least two zygotes. This state may result in the induction of donor-specific TRANSPLANTATION TOLERANCE.
A multilineage cell growth factor secreted by LYMPHOCYTES; EPITHELIAL CELLS; and ASTROCYTES which stimulates clonal proliferation and differentiation of various types of blood and tissue cells.
Transfer of HEMATOPOIETIC STEM CELLS from BONE MARROW or BLOOD between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS). Hematopoietic stem cell transplantation has been used as an alternative to BONE MARROW TRANSPLANTATION in the treatment of a variety of neoplasms.
Diseases of BONES.
The process of bone formation. Histogenesis of bone including ossification.
The production of red blood cells (ERYTHROCYTES). In humans, erythrocytes are produced by the YOLK SAC in the first trimester; by the liver in the second trimester; by the BONE MARROW in the third trimester and after birth. In normal individuals, the erythrocyte count in the peripheral blood remains relatively constant implying a balance between the rate of erythrocyte production and rate of destruction.
A form of anemia in which the bone marrow fails to produce adequate numbers of peripheral blood elements.
A single, unpaired primary lymphoid organ situated in the MEDIASTINUM, extending superiorly into the neck to the lower edge of the THYROID GLAND and inferiorly to the fourth costal cartilage. It is necessary for normal development of immunologic function early in life. By puberty, it begins to involute and much of the tissue is replaced by fat.
The clinical entity characterized by anorexia, diarrhea, loss of hair, leukopenia, thrombocytopenia, growth retardation, and eventual death brought about by the GRAFT VS HOST REACTION.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.
Clonal expansion of myeloid blasts in bone marrow, blood, and other tissue. Myeloid leukemias develop from changes in cells that normally produce NEUTROPHILS; BASOPHILS; EOSINOPHILS; and MONOCYTES.
Elements of limited time intervals, contributing to particular results or situations.
Renewal or repair of lost bone tissue. It excludes BONY CALLUS formed after BONE FRACTURES but not yet replaced by hard bone.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
Bone-forming cells which secrete an EXTRACELLULAR MATRIX. HYDROXYAPATITE crystals are then deposited into the matrix to form bone.
A glycoprotein of MW 25 kDa containing internal disulfide bonds. It induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and functionally activates mature blood neutrophils. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines.
The developmental history of specific differentiated cell types as traced back to the original STEM CELLS in the embryo.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
A hematopoietic growth factor and the ligand of the cell surface c-kit protein (PROTO-ONCOGENE PROTEINS C-KIT). It is expressed during embryogenesis and is a growth factor for a number of cell types including the MAST CELLS and the MELANOCYTES in addition to the HEMATOPOIETIC STEM CELLS.
Family of RNA viruses that infects birds and mammals and encodes the enzyme reverse transcriptase. The family contains seven genera: DELTARETROVIRUS; LENTIVIRUS; RETROVIRUSES TYPE B, MAMMALIAN; ALPHARETROVIRUS; GAMMARETROVIRUS; RETROVIRUSES TYPE D; and SPUMAVIRUS. A key feature of retrovirus biology is the synthesis of a DNA copy of the genome which is integrated into cellular DNA. After integration it is sometimes not expressed but maintained in a latent state (PROVIRUSES).
Form of leukemia characterized by an uncontrolled proliferation of the myeloid lineage and their precursors (MYELOID PROGENITOR CELLS) in the bone marrow and other sites.
An acidic glycoprotein of MW 23 kDa with internal disulfide bonds. The protein is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. GM-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages.
Clonal hematopoietic stem cell disorders characterized by dysplasia in one or more hematopoietic cell lineages. They predominantly affect patients over 60, are considered preleukemic conditions, and have high probability of transformation into ACUTE MYELOID LEUKEMIA.
The number of CELLS of a specific kind, usually measured per unit volume or area of sample.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
The cells found in the body fluid circulating throughout the CARDIOVASCULAR SYSTEM.
The survival of a graft in a host, the factors responsible for the survival and the changes occurring within the graft during growth in the host.
An individual that contains cell populations derived from different zygotes.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
A malignancy of mature PLASMA CELLS engaging in monoclonal immunoglobulin production. It is characterized by hyperglobulinemia, excess Bence-Jones proteins (free monoclonal IMMUNOGLOBULIN LIGHT CHAINS) in the urine, skeletal destruction, bone pain, and fractures. Other features include ANEMIA; HYPERCALCEMIA; and RENAL INSUFFICIENCY.
The grafting of bone from a donor site to a recipient site.
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
The longest and largest bone of the skeleton, it is situated between the hip and the knee.
Process of classifying cells of the immune system based on structural and functional differences. The process is commonly used to analyze and sort T-lymphocytes into subsets based on CD antigens by the technique of flow cytometry.
Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
The number of WHITE BLOOD CELLS per unit volume in venous BLOOD. A differential leukocyte count measures the relative numbers of the different types of white cells.
A protein-tyrosine kinase receptor that is specific for STEM CELL FACTOR. This interaction is crucial for the development of hematopoietic, gonadal, and pigment stem cells. Genetic mutations that disrupt the expression of PROTO-ONCOGENE PROTEINS C-KIT are associated with PIEBALDISM, while overexpression or constitutive activation of the c-kit protein-tyrosine kinase is associated with tumorigenesis.
Extracellular substance of bone tissue consisting of COLLAGEN fibers, ground substance, and inorganic crystalline minerals and salts.
The number of LEUKOCYTES and ERYTHROCYTES per unit volume in a sample of venous BLOOD. A complete blood count (CBC) also includes measurement of the HEMOGLOBIN; HEMATOCRIT; and ERYTHROCYTE INDICES.
Deficiency of all three cell elements of the blood, erythrocytes, leukocytes and platelets.
The cells in the erythroid series derived from MYELOID PROGENITOR CELLS or from the bi-potential MEGAKARYOCYTE-ERYTHROID PROGENITOR CELLS which eventually give rise to mature RED BLOOD CELLS. The erythroid progenitor cells develop in two phases: erythroid burst-forming units (BFU-E) followed by erythroid colony-forming units (CFU-E); BFU-E differentiate into CFU-E on stimulation by ERYTHROPOIETIN, and then further differentiate into ERYTHROBLASTS when stimulated by other factors.
Antibodies produced by a single clone of cells.
Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.
Proteins prepared by recombinant DNA technology.
A group of lymphocyte surface antigens located on mouse LYMPHOCYTES. Specific Ly antigens are useful markers for distinguishing subpopulations of lymphocytes.
Transplantation between genetically identical individuals, i.e., members of the same species with identical histocompatibility antigens, such as monozygotic twins, members of the same inbred strain, or members of a hybrid population produced by crossing certain inbred strains.
These growth factors comprise a family of hematopoietic regulators with biological specificities defined by their ability to support proliferation and differentiation of blood cells of different lineages. ERYTHROPOIETIN and the COLONY-STIMULATING FACTORS belong to this family. Some of these factors have been studied and used in the treatment of chemotherapy-induced neutropenia, myelodysplastic syndromes, and bone marrow failure syndromes.
Clonal hematopoetic disorder caused by an acquired genetic defect in PLURIPOTENT STEM CELLS. It starts in MYELOID CELLS of the bone marrow, invades the blood and then other organs. The condition progresses from a stable, more indolent, chronic phase (LEUKEMIA, MYELOID, CHRONIC PHASE) lasting up to 7 years, to an advanced phase composed of an accelerated phase (LEUKEMIA, MYELOID, ACCELERATED PHASE) and BLAST CRISIS.
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
Precursor of an alkylating nitrogen mustard antineoplastic and immunosuppressive agent that must be activated in the LIVER to form the active aldophosphamide. It has been used in the treatment of LYMPHOMA and LEUKEMIA. Its side effect, ALOPECIA, has been used for defleecing sheep. Cyclophosphamide may also cause sterility, birth defects, mutations, and cancer.
A transmembrane protein belonging to the tumor necrosis factor superfamily that specifically binds RECEPTOR ACTIVATOR OF NUCLEAR FACTOR-KAPPA B and OSTEOPROTEGERIN. It plays an important role in regulating OSTEOCLAST differentiation and activation.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
A mononuclear phagocyte colony-stimulating factor (M-CSF) synthesized by mesenchymal cells. The compound stimulates the survival, proliferation, and differentiation of hematopoietic cells of the monocyte-macrophage series. M-CSF is a disulfide-bonded glycoprotein dimer with a MW of 70 kDa. It binds to a specific high affinity receptor (RECEPTOR, MACROPHAGE COLONY-STIMULATING FACTOR).
A group of differentiation surface antigens, among the first to be discovered on thymocytes and T-lymphocytes. Originally identified in the mouse, they are also found in other species including humans, and are expressed on brain neurons and other cells.
Stem cells derived from HEMATOPOIETIC STEM CELLS. Derived from these myeloid progenitor cells are the MEGAKARYOCYTES; ERYTHROID CELLS; MYELOID CELLS; and some DENDRITIC CELLS.
Glycoprotein hormone, secreted chiefly by the KIDNEY in the adult and the LIVER in the FETUS, that acts on erythroid stem cells of the BONE MARROW to stimulate proliferation and differentiation.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES).
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Bone-marrow-derived, non-hematopoietic cells that support HEMATOPOETIC STEM CELLS. They have also been isolated from other organs and tissues such as UMBILICAL CORD BLOOD, umbilical vein subendothelium, and WHARTON JELLY. These cells are considered to be a source of multipotent stem cells because they include subpopulations of mesenchymal stem cells.
High-molecular weight glycoproteins uniquely expressed on the surface of LEUKOCYTES and their hemopoietic progenitors. They contain a cytoplasmic protein tyrosine phosphatase activity which plays a role in intracellular signaling from the CELL SURFACE RECEPTORS. The CD45 antigens occur as multiple isoforms that result from alternative mRNA splicing and differential usage of three exons.
Established cell cultures that have the potential to propagate indefinitely.
Formation of MYELOID CELLS from the pluripotent HEMATOPOIETIC STEM CELLS in the BONE MARROW via MYELOID STEM CELLS. Myelopoiesis generally refers to the production of leukocytes in blood, such as MONOCYTES and GRANULOCYTES. This process also produces precursor cells for MACROPHAGE and DENDRITIC CELLS found in the lymphoid tissue.
Mice bearing mutant genes which are phenotypically expressed in the animals.
Conditions in which the abnormalities in the peripheral blood or bone marrow represent the early manifestations of acute leukemia, but in which the changes are not of sufficient magnitude or specificity to permit a diagnosis of acute leukemia by the usual clinical criteria.
Surgical removal of the thymus gland. (Dorland, 28th ed)
An alkylating agent having a selective immunosuppressive effect on BONE MARROW. It has been used in the palliative treatment of chronic myeloid leukemia (MYELOID LEUKEMIA, CHRONIC), but although symptomatic relief is provided, no permanent remission is brought about. According to the Fourth Annual Report on Carcinogens (NTP 85-002, 1985), busulfan is listed as a known carcinogen.
A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states.
A humoral factor that stimulates the production of thrombocytes (BLOOD PLATELETS). Thrombopoietin stimulates the proliferation of bone marrow MEGAKARYOCYTES and their release of blood platelets. The process is called THROMBOPOIESIS.
The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Leukemia induced experimentally in animals by exposure to leukemogenic agents, such as VIRUSES; RADIATION; or by TRANSPLANTATION of leukemic tissues.
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
Synthetic or natural materials for the replacement of bones or bone tissue. They include hard tissue replacement polymers, natural coral, hydroxyapatite, beta-tricalcium phosphate, and various other biomaterials. The bone substitutes as inert materials can be incorporated into surrounding tissue or gradually replaced by original tissue.
A neoplasm characterized by abnormalities of the lymphoid cell precursors leading to excessive lymphoblasts in the marrow and other organs. It is the most common cancer in children and accounts for the vast majority of all childhood leukemias.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
Preparative treatment of transplant recipient with various conditioning regimens including radiation, immune sera, chemotherapy, and/or immunosuppressive agents, prior to transplantation. Transplantation conditioning is very common before bone marrow transplantation.
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
Immature, nucleated ERYTHROCYTES occupying the stage of ERYTHROPOIESIS that follows formation of ERYTHROID PRECURSOR CELLS and precedes formation of RETICULOCYTES. The normal series is called normoblasts. Cells called MEGALOBLASTS are a pathologic series of erythroblasts.
Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.
A CXC chemokine that is chemotactic for T-LYMPHOCYTES and MONOCYTES. It has specificity for CXCR4 RECEPTORS. Two isoforms of CXCL12 are produced by alternative mRNA splicing.
The second longest bone of the skeleton. It is located on the medial side of the lower leg, articulating with the FIBULA laterally, the TALUS distally, and the FEMUR proximally.
Conditions which cause proliferation of hemopoietically active tissue or of tissue which has embryonic hemopoietic potential. They all involve dysregulation of multipotent MYELOID PROGENITOR CELLS, most often caused by a mutation in the JAK2 PROTEIN TYROSINE KINASE.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
The process of generating white blood cells (LEUKOCYTES) from the pluripotent HEMATOPOIETIC STEM CELLS of the BONE MARROW. There are two significant pathways to generate various types of leukocytes: MYELOPOIESIS, in which leukocytes in the blood are derived from MYELOID STEM CELLS, and LYMPHOPOIESIS, in which leukocytes of the lymphatic system (LYMPHOCYTES) are generated from lymphoid stem cells.
Mice homozygous for the mutant autosomal recessive gene "scid" which is located on the centromeric end of chromosome 16. These mice lack mature, functional lymphocytes and are thus highly susceptible to lethal opportunistic infections if not chronically treated with antibiotics. The lack of B- and T-cell immunity resembles severe combined immunodeficiency (SCID) syndrome in human infants. SCID mice are useful as animal models since they are receptive to implantation of a human immune system producing SCID-human (SCID-hu) hematochimeric mice.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Individuals supplying living tissue, organs, cells, blood or blood components for transfer or transplantation to histocompatible recipients.
The physiological renewal, repair, or replacement of tissue.
The release of stem cells from the bone marrow into the peripheral blood circulation for the purpose of leukapheresis, prior to stem cell transplantation. Hematopoietic growth factors or chemotherapeutic agents often are used to stimulate the mobilization.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Drugs used to protect against ionizing radiation. They are usually of interest for use in radiation therapy but have been considered for other, e.g. military, purposes.
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
The formation and development of blood cells outside the BONE MARROW, as in the SPLEEN; LIVER; or LYMPH NODES.
Formation of LYMPHOCYTES and PLASMA CELLS from the lymphoid stem cells which develop from the pluripotent HEMATOPOIETIC STEM CELLS in the BONE MARROW. These lymphoid stem cells differentiate into T-LYMPHOCYTES; B-LYMPHOCYTES; PLASMA CELLS; or NK-cells (KILLER CELLS, NATURAL) depending on the organ or tissues (LYMPHOID TISSUE) to which they migrate.
Abnormal number or structure of chromosomes. Chromosome aberrations may result in CHROMOSOME DISORDERS.
An immunological attack mounted by a graft against the host because of tissue incompatibility when immunologically competent cells are transplanted to an immunologically incompetent host; the resulting clinical picture is that of GRAFT VS HOST DISEASE.
An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.1.
Immunosuppression by reduction of circulating lymphocytes or by T-cell depletion of bone marrow. The former may be accomplished in vivo by thoracic duct drainage or administration of antilymphocyte serum. The latter is performed ex vivo on bone marrow before its transplantation.
The blood-making organs and tissues, principally the bone marrow and lymph nodes.
Antigens expressed primarily on the membranes of living cells during sequential stages of maturation and differentiation. As immunologic markers they have high organ and tissue specificity and are useful as probes in studies of normal cell development as well as neoplastic transformation.
The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.
The classes of BONE MARROW-derived blood cells in the monocytic series (MONOCYTES and their precursors) and granulocytic series (GRANULOCYTES and their precursors).
Transfer of MESENCHYMAL STEM CELLS between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS).
Disease having a short and relatively severe course.
The degree of antigenic similarity between the tissues of different individuals, which determines the acceptance or rejection of allografts.
The transfer of STEM CELLS from one individual to another within the same species (TRANSPLANTATION, HOMOLOGOUS) or between species (XENOTRANSPLANTATION), or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS). The source and location of the stem cells determines their potency or pluripotency to differentiate into various cell types.
Breaks in bones.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
Bone-growth regulatory factors that are members of the transforming growth factor-beta superfamily of proteins. They are synthesized as large precursor molecules which are cleaved by proteolytic enzymes. The active form can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins.
Specialized forms of antibody-producing B-LYMPHOCYTES. They synthesize and secrete immunoglobulin. They are found only in lymphoid organs and at sites of immune responses and normally do not circulate in the blood or lymph. (Rosen et al., Dictionary of Immunology, 1989, p169 & Abbas et al., Cellular and Molecular Immunology, 2d ed, p20)
A lymphohematopoietic cytokine that plays a role in regulating the proliferation of ERYTHROID PRECURSOR CELLS. It induces maturation of MEGAKARYOCYTES which results in increased production of BLOOD PLATELETS. Interleukin-11 was also initially described as an inhibitor of ADIPOGENESIS of cultured preadipocytes.
Methods for maintaining or growing CELLS in vitro.
A potent osteoinductive protein that plays a critical role in the differentiation of osteoprogenitor cells into OSTEOBLASTS.
Induction and quantitative measurement of chromosomal damage leading to the formation of micronuclei (MICRONUCLEI, CHROMOSOME-DEFECTIVE) in cells which have been exposed to genotoxic agents or IONIZING RADIATION.
Signal molecules that are involved in the control of cell growth and differentiation.
Mapping of the KARYOTYPE of a cell.
Evaluation undertaken to assess the results or consequences of management and procedures used in combating disease in order to determine the efficacy, effectiveness, safety, and practicability of these interventions in individual cases or series.
Group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. It is inherited as an X-linked or autosomal recessive defect. Mutations occurring in many different genes cause human Severe Combined Immunodeficiency (SCID).
A general term for various neoplastic diseases of the lymphoid tissue.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.
A de novo myeloproliferation arising from an abnormal stem cell. It is characterized by the replacement of bone marrow by fibrous tissue, a process that is mediated by CYTOKINES arising from the abnormal clone.
Adherence of cells to surfaces or to other cells.
Transference of cells within an individual, between individuals of the same species, or between individuals of different species.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
The development of new BLOOD VESSELS during the restoration of BLOOD CIRCULATION during the healing process.
Leukemia associated with HYPERPLASIA of the lymphoid tissues and increased numbers of circulating malignant LYMPHOCYTES and lymphoblasts.
Disorders of the blood and blood forming tissues.
An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.2.
The relationship between the dose of an administered drug and the response of the organism to the drug.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Process by which organic tissue becomes hardened by the physiologic deposit of calcium salts.
A cytokine that stimulates the growth and differentiation of B-LYMPHOCYTES and is also a growth factor for HYBRIDOMAS and plasmacytomas. It is produced by many different cells including T-LYMPHOCYTES; MONOCYTES; and FIBROBLASTS.
Identification of the major histocompatibility antigens of transplant DONORS and potential recipients, usually by serological tests. Donor and recipient pairs should be of identical ABO blood group, and in addition should be matched as closely as possible for HISTOCOMPATIBILITY ANTIGENS in order to minimize the likelihood of allograft rejection. (King, Dictionary of Genetics, 4th ed)
The transfer of lymphocytes from a donor to a recipient or reinfusion to the donor.
Glycoproteins found on the membrane or surface of cells.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
The grafting of skin in humans or animals from one site to another to replace a lost portion of the body surface skin.
Vitamin K-dependent calcium-binding protein synthesized by OSTEOBLASTS and found primarily in BONES. Serum osteocalcin measurements provide a noninvasive specific marker of bone metabolism. The protein contains three residues of the amino acid gamma-carboxyglutamic acid (Gla), which, in the presence of CALCIUM, promotes binding to HYDROXYAPATITE and subsequent accumulation in BONE MATRIX.
The specific failure of a normally responsive individual to make an immune response to a known antigen. It results from previous contact with the antigen by an immunologically immature individual (fetus or neonate) or by an adult exposed to extreme high-dose or low-dose antigen, or by exposure to radiation, antimetabolites, antilymphocytic serum, etc.
A general term for the complex phenomena involved in allo- and xenograft rejection by a host and graft vs host reaction. Although the reactions involved in transplantation immunology are primarily thymus-dependent phenomena of cellular immunity, humoral factors also play a part in late rejection.
Deliberate prevention or diminution of the host's immune response. It may be nonspecific as in the administration of immunosuppressive agents (drugs or radiation) or by lymphocyte depletion or may be specific as in desensitization or the simultaneous administration of antigen and immunosuppressive drugs.
Reduction of bone mass without alteration in the composition of bone, leading to fractures. Primary osteoporosis can be of two major types: postmenopausal osteoporosis (OSTEOPOROSIS, POSTMENOPAUSAL) and age-related or senile osteoporosis.
The effects of ionizing and nonionizing radiation upon living organisms, organs and tissues, and their constituents, and upon physiologic processes. It includes the effect of irradiation on food, drugs, and chemicals.
Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.
Experimentally produced harmful effects of ionizing or non-ionizing RADIATION in CHORDATA animals.
Bone marrow-derived lymphocytes that possess cytotoxic properties, classically directed against transformed and virus-infected cells. Unlike T CELLS; and B CELLS; NK CELLS are not antigen specific. The cytotoxicity of natural killer cells is determined by the collective signaling of an array of inhibitory and stimulatory CELL SURFACE RECEPTORS. A subset of T-LYMPHOCYTES referred to as NATURAL KILLER T CELLS shares some of the properties of this cell type.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Specialized tissues that are components of the lymphatic system. They provide fixed locations within the body where a variety of LYMPHOCYTES can form, mature and multiply. The lymphoid tissues are connected by a network of LYMPHATIC VESSELS.
Mature osteoblasts that have become embedded in the BONE MATRIX. They occupy a small cavity, called lacuna, in the matrix and are connected to adjacent osteocytes via protoplasmic projections called canaliculi.
Toxic, volatile, flammable liquid hydrocarbon byproduct of coal distillation. It is used as an industrial solvent in paints, varnishes, lacquer thinners, gasoline, etc. Benzene causes central nervous system damage acutely and bone marrow damage chronically and is carcinogenic. It was formerly used as parasiticide.
A pyrimidine nucleoside analog that is used mainly in the treatment of leukemia, especially acute non-lymphoblastic leukemia. Cytarabine is an antimetabolite antineoplastic agent that inhibits the synthesis of DNA. Its actions are specific for the S phase of the cell cycle. It also has antiviral and immunosuppressant properties. (From Martindale, The Extra Pharmacopoeia, 30th ed, p472)
Culture media containing biologically active components obtained from previously cultured cells or tissues that have released into the media substances affecting certain cell functions (e.g., growth, lysis).
2'-Deoxyuridine. An antimetabolite that is converted to deoxyuridine triphosphate during DNA synthesis. Laboratory suppression of deoxyuridine is used to diagnose megaloblastic anemias due to vitamin B12 and folate deficiencies.
The treatment of a disease or condition by several different means simultaneously or sequentially. Chemoimmunotherapy, RADIOIMMUNOTHERAPY, chemoradiotherapy, cryochemotherapy, and SALVAGE THERAPY are seen most frequently, but their combinations with each other and surgery are also used.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
A tumor necrosis factor receptor family member that is specific for RANK LIGAND and plays a role in bone homeostasis by regulating osteoclastogenesis. It is also expressed on DENDRITIC CELLS where it plays a role in regulating dendritic cell survival. Signaling by the activated receptor occurs through its association with TNF RECEPTOR-ASSOCIATED FACTORS.

Thymic selection by a single MHC/peptide ligand: autoreactive T cells are low-affinity cells. (1/12182)

In H2-M- mice, the presence of a single peptide, CLIP, bound to MHC class II molecules generates a diverse repertoire of CD4+ cells. In these mice, typical self-peptides are not bound to class II molecules, with the result that a very high proportion of H2-M- CD4+ cells are responsive to the various peptides displayed on normal MHC-compatible APC. We show here, however, that such "self" reactivity is controlled by low-affinity CD4+ cells. These cells give spectacularly high proliferative responses but are virtually unreactive in certain other assays, e.g., skin graft rejection; responses to MHC alloantigens, by contrast, are intense in all assays. Possible explanations for why thymic selection directed to a single peptide curtails self specificity without affecting alloreactivity are discussed.  (+info)

gp49B1 inhibits IgE-initiated mast cell activation through both immunoreceptor tyrosine-based inhibitory motifs, recruitment of src homology 2 domain-containing phosphatase-1, and suppression of early and late calcium mobilization. (2/12182)

We define by molecular, pharmacologic, and physiologic approaches the proximal mechanism by which the immunoglobulin superfamily member gp49B1 inhibits mast cell activation mediated by the high affinity Fc receptor for IgE (FcepsilonRI). In rat basophilic leukemia-2H3 cells expressing transfected mouse gp49B1, mutation of tyrosine to phenylalanine in either of the two immunoreceptor tyrosine-based inhibitory motifs of the gp49B1 cytoplasmic domain partially suppressed gp49B1-mediated inhibition of exocytosis, whereas mutation of both abolished inhibitory capacity. Sodium pervanadate elicited tyrosine phosphorylation of native gp49B1 and association of the tyrosine phosphatases src homology 2 domain-containing phosphatase-1 (SHP-1) and SHP-2 in mouse bone marrow-derived mast cells (mBMMCs). SHP-1 associated transiently with gp49B1 within 1 min after coligation of gp49B1 with cross-linked FcepsilonRI in mBMMCs. SHP-1-deficient mBMMCs exhibited a partial loss of gp49B1-mediated inhibition of FcepsilonRI-induced exocytosis at concentrations of IgE providing optimal exocytosis, revealing a central, but not exclusive, SHP-1 requirement in the counter-regulatory pathway. Coligation of gp49B1 with cross-linked FcepsilonRI on mBMMCs inhibited early release of calcium from intracellular stores and subsequent influx of extracellular calcium, consistent with SHP-1 participation. Because exocytosis is complete within 2 min in mBMMCs, our studies establish a role for SHP-1 in the initial counter-regulatory cellular responses whereby gp49B1 immunoreceptor tyrosine-based inhibition motifs rapidly transmit inhibition of FcepsilonRI-mediated exocytosis.  (+info)

Expression of neurotrophins and their receptors in human bone marrow. (3/12182)

The expression of neurotrophins and their receptors, the low-affinity nerve growth factor receptor (p75LNGFR) and the Trk receptors (TrkA, TrkB, and TrkC), was investigated in human bone marrow from 16 weeks fetal age to adulthood. Using reverse transcription-polymerase chain reaction, all transcripts encoding for catalytic and truncated human TrkB or TrkC receptors were detected together with trkAI transcripts, whereas trkAII transcripts were found only in control nerve tissues. Transcripts for the homologue of the rat truncated TrkC(ic113) receptor were identified for the first time in human tissue. Stromal adventitial reticular cells were found immunoreactive for all neutrophin receptors. In contrast, hematopoietic cell types were not immunoreactive for p75LNGFR but showed immunoreactivity for one or several Trk receptors. TrkA immunoreactivity was found in immature erythroblasts. Catalytic TrkB immunoreactivity was observed in eosinophilic metamyelocytes and polymorphonuclear cells. Truncated TrkB immunoreactivity was found in erythroblasts and megacaryocytes. Immunoreactivity for both catalytic and truncated TrkC receptor was observed in promyelocytes, myelocytes, some polymorphonuclear cells and megacaryocytes. Neutrophin transcript levels appeared higher at fetal than at adult stages, no variation in Trk family transcript levels was observed. The local expression of neurotrophin genes suggests a wide range of paracrine and/or autocrine mode of action through their corresponding receptors within the bone marrow.  (+info)

Disproportionate recruitment of CD8+ T cells into the central nervous system by professional antigen-presenting cells. (4/12182)

Inappropriate immune responses, thought to exacerbate or even to initiate several types of central nervous system (CNS) neuropathology, could arise from failures by either the CNS or the immune system. The extent that the inappropriate appearance of antigen-presenting cell (APC) function contributes to CNS inflammation and pathology is still under debate. Therefore, we characterized the response initiated when professional APCs (dendritic cells) presenting non-CNS antigens were injected into the CNS. These dendritic cells expressed numerous T-cell chemokines, but only in the presence of antigen did leukocytes accumulate in the ventricles, meninges, sub-arachnoid spaces, and injection site. Within the CNS parenchyma, the injected dendritic cells migrated preferentially into the white matter tracts, yet only a small percentage of the recruited leukocytes entered the CNS parenchyma, and then only in the white matter tracts. Although T-cell recruitment was antigen specific and thus mediated by CD4+ T cells in the models used here, CD8+ T cells accumulated in numbers equal to or greater than that of CD4+ T cells. Few of the recruited T cells expressed activation markers (CD25 and VLA-4), and those that did were primarily in the meninges, injection site, ventricles, and perivascular spaces but not in the parenchyma. These results indicate that 1) the CNS modulates the cellular composition and activation states of responding T-cell populations and that 2) myelin-restricted inflammation need not be initiated by a myelin-specific antigen.  (+info)

Detection of Kaposi's sarcoma herpesvirus DNA sequences in multiple myeloma bone marrow stromal cells. (5/12182)

Whether Kaposi's sarcoma herpesvirus (KSHV) is associated with multiple myeloma (MM) remains controversial. We assayed for KSHV DNA sequences in long-term bone marrow stromal cells (BMSCs) from 26 patients with MM and 4 normal donors. Polymerase chain reaction (PCR) using primers which amplify a KSHV gene sequence to yield a 233-bp fragment (KS330233 within open reading frame 26) was negative in all cases. Aliquots of these PCR products were used as templates in subsequent nested PCR, with primers that amplify a 186-bp product internal to KS330233. BMSCs from 24 of 26 (92%) patients with MM and 1 of 4 normal donors were KSHV PCR+. DNA sequence analyses showed interpatient specific mutations (2 to 3 bp). Both Southern blot and sequence analyses confirmed the specificity of PCR results. The presence of the KSHV gene sequences was further confirmed by amplifying T 1.1 (open reading frame [ORF] K7) and viral cyclin D (ORF 72), two other domains within the KSHV genome. Immunohistochemical studies of KSHV PCR+ MM BMSCs demonstrate expression of dendritic cell (DC) lineage markers (CD68, CD83, and fascin). Serological studies for the presence of KSHV lytic or latent antibodies were performed using sera from 53 MM patients, 12 normal donors, and 5 human immunodeficiency virus (HIV)/KSHV+ patients. No lytic or latent antibodies were present in sera from either MM patients or normal donors. Taken together, these findings show that KSHV DNA sequences are detectable in BMSCs from the majority of MM patients, but that serologic responses to KSHV are not present. Ongoing studies are defining whether the lack of antibody response is caused by the absence of ongoing infection, the presence of a novel viral strain associated with MM, or underlying immunodeficiency in these patients.  (+info)

Bone marrow and peripheral blood dendritic cells from patients with multiple myeloma are phenotypically and functionally normal despite the detection of Kaposi's sarcoma herpesvirus gene sequences. (6/12182)

Multiple myeloma (MM) cells express idiotypic proteins and other tumor-associated antigens which make them ideal targets for novel immunotherapeutic approaches. However, recent reports show the presence of Kaposi's sarcoma herpesvirus (KSHV) gene sequences in bone marrow dendritic cells (BMDCs) in MM, raising concerns regarding their antigen-presenting cell (APC) function. In the present study, we sought to identify the ideal source of DCs from MM patients for use in vaccination approaches. We compared the relative frequency, phenotype, and function of BMDCs or peripheral blood dendritic cells (PBDCs) from MM patients versus normal donors. DCs were derived by culture of mononuclear cells in the presence of granulocyte-macrophage colony-stimulating factor and interleukin-4. The yield as well as the pattern and intensity of Ag (HLA-DR, CD40, CD54, CD80, and CD86) expression were equivalent on DCs from BM or PB of MM patients versus normal donors. Comparison of PBDCs versus BMDCs showed higher surface expression of HLA-DR (P =.01), CD86 (P =. 0003), and CD14 (P =.04) on PBDCs. APC function, assessed using an allogeneic mixed lymphocyte reaction (MLR), demonstrated equivalent T-cell proliferation triggered by MM versus normal DCs. Moreover, no differences in APC function were noted in BMDCs compared with PBDCs. Polymerase chain reaction (PCR) analysis of genomic DNA from both MM patient and normal donor DCs for the 233-bp KSHV gene sequence (KS330233) was negative, but nested PCR to yield a final product of 186 bp internal to KS330233 was positive in 16 of 18 (88.8%) MM BMDCs, 3 of 8 (37.5%) normal BMDCs, 1 of 5 (20%) MM PBDCs, and 2 of 6 (33.3%) normal donor PBDCs. Sequencing of 4 MM patient PCR products showed 96% to 98% homology to the published KSHV gene sequence, with patient specific mutations ruling out PCR artifacts or contamination. In addition, KHSV-specific viral cyclin D (open reading frame [ORF] 72) was amplified in 2 of 5 MM BMDCs, with sequencing of the ORF 72 amplicon revealing 91% and 92% homology to the KSHV viral cyclin D sequence. These sequences again demonstrated patient specific mutations, ruling out contamination. Therefore, our studies show that PB appears to be the preferred source of DCs for use in vaccination strategies due to the ready accessibility and phenotypic profile of PBDCs, as well as the comparable APC function and lower detection rate of KSHV gene sequences compared with BMDCs. Whether active KSHV infection is present and important in the pathophysiology of MM remains unclear; however, our study shows that MMDCs remain functional despite the detection of KSHV gene sequences.  (+info)

Effects of short-term administration of G-CSF (filgrastim) on bone marrow progenitor cells: analysis of serial marrow samples from normal donors. (7/12182)

To determine the effect of G-CSF administration on both the total number of CD34+ cells and the primitive CD34+ subsets in bone marrow (BM), we have analyzed BM samples serially obtained from 10 normal donors in steady-state and during G-CSF treatment. Filgrastim was administered subcutaneously at a dosage of 10 microg/kg/day (n = 7) or 10 microg/kg/12 h (n = 3) for 4 consecutive days. Peripheral blood sampling and BM aspirates were performed on day 1 (just before G-CSF administration), day 3 (after 2 days of G-CSF), and day 5 (after 4 days of G-CSF). During G-CSF administration, a significant increase in the total number of BM nucleated cells was observed. The percentage (range) of CD34+ cells decreased in BM from a median of 0.88 (0.47-1.44) on day 1 to 0.57 (0.32-1.87), and to 0.42 (0.16-0.87) on days 3 and 5, respectively. We observed a slight increase in the total number of BM CD34+ cells on day 3 (0.66 x 10(9)/l (0.13-0.77)), and a decrease on day 5 (0.23 x 10(9)/l (0.06-1.23)) as compared with steady-state (0.40 x 10(9)/l (0.06-1.68)). The proportion of primitive BM hematopoietic progenitor cells (CD34+CD38-, CD34+HLA-DR-, CD34+CD117-) decreased during G-CSF administration. In parallel, a significant increase in the total number of CD34+ cells in peripheral blood was observed, achieving the maximum value on day 5. These results suggest that in normal subjects the administration of G-CSF for 5 days may reduce the number of progenitor cells in BM, particularly the most primitive ones.  (+info)

Immunoregulatory cytokines in bone marrow and peripheral blood stem cell products. (8/12182)

In these studies, we compared the phenotype, function, and expression of type 1, type 2, and monocyte-associated cytokine mRNA transcripts in autologous bone marrow (BM) and growth factor-mobilized peripheral blood stem cell (PSC) products. These studies demonstrate that lymphocytes and monocytes in stem cell products are abnormally activated, expressing significantly higher levels of interleukin (IL)-2, 4 and 10, interferon gamma (IFN-gamma), and tumor necrosis factor alpha (TNF-alpha), but not IL-8, as compared to normal peripheral blood mononuclear cells (PBMC). In addition, the levels of IL-2, IL-10 and TNF-alpha are significantly higher in mobilized PSC as compared to BM products. The high cytokine levels are unexpected as T cell function in stem cell products is depressed. PSC products have high levels of T cell inhibitory activity, which directly correlates with IL-10 expression, both of which are mechanisms that might be involved in the immune dysfunction within stem cell products used for autologous stem cell transplantation. These data demonstrate that: (1) immune cells in autologous BM and PSC products are activated with the expression of high levels of type 1 and type 2 cytokines as well as monokines; (2) PSC products contain a high frequency of monocytes which mediate T cell inhibitory activity; and (3) despite the high levels of cytokine expression, T cell function in stem cell products is depressed. The significance of these immune abnormalities within stem cell products for myeloid and lymphoid recovery following autologous stem cell transplantation remains to be determined.  (+info)

These data suggest that the propensity of prostate cancer cells to establish themselves in bone is due, at least in part, to their preferential adhesion to human bone marrow endothelial cells.
Transforming growth factor beta (TGF-beta), an immunomodulator, has inhibitory as well as stimulatory effects on bone marrow cells. In this study, we demonstrate that TGF-beta 1 also is a bidirectional modulator of CSF receptor expression on murine bone marrow cells. TGF-beta 1 up-regulated granulocyte-macrophage (GM)-CSF receptor expression in a time- and dose-dependent manner, with a maximum up-regulation of 64% by 48 h at 20 ng/ml. In contrast, TGF-beta 1 down-modulated IL-3 and CSF-1 receptor expression by 54 and 55%, respectively, by 24 h. TGF-beta 1 did not affect G-CSF receptor expression, in agreement with its inability to affect G-CSF-induced proliferation. The CSF receptor modulation induced by TGF-beta 1 preceded its effects on CSF-stimulated proliferation. The effects of TGF-beta on CSF receptor expression were isoform dependent, thus TGF-beta 3 was a 10-fold more potent inhibitor of both IL-3-induced colony formation and IL-3 receptor expression than TGF-beta 1, whereas TGF-beta 1 was a
TY - JOUR. T1 - Lack of Development of Thermotolerance in Early Progenitors of Murine Bone Marrow Cells. AU - Mivechi, Nahid F.. AU - Li, Gloria C.. N1 - Copyright: Copyright 2016 Elsevier B.V., All rights reserved.. PY - 1986/1/1. Y1 - 1986/1/1. N2 - We have studied the sensitivities of four hematopoietic stem cell types to heat stress as well as their abilities to develop thermotolerance. Granulocyte-macrophage colony forming units were the most heat resistant bone marrow progenitors tested. Of the erythroid progenitors tested, erythrocyte colony forming units were more resistant than the two more primitive erythrocyte burst forming units. To determine their ability to develop thermotolerance, hematopoietic precursors were heated in vivo at 43C for 30 min. At various times thereafter the hematopoietic stem cells were flushed from female C3Hf/Sed mouse preheated tibia. The bone marrow cell suspensions were then heated in vitro and plated for colony formation. The four stem cell precursors ...
Mouse bone marrow cells are responsive to growth factors and can be used to derive macrophages, dendritic cells, mast cells, and other cell types.. We isolate bone marrow cells from young mice using minimal processing techniques to avoid cell loss. Mouse bone marrow cells have the potential to generate all hematopoietic cell types.. ...
TY - JOUR. T1 - Upregulation of IL-5 receptor expression on bone marrow-derived CD34+ cells from patients with asthma.. AU - Chou, C. L.. AU - Wang, C. H.. AU - Kuo, H. P.. PY - 1999/1/1. Y1 - 1999/1/1. N2 - BACKGROUND: Interleukin-5 (IL-5) is a potent eosinophilopoietic factor implicated in the chronic inflammatory cell accumulation accompanying bronchial asthma. We studied the expression of the IL-5 receptor alpha-subunit (IL-5R alpha) on bone marrow-derived cluster of differentiation molecule 34 positive (CD34+) progenitor cells in asthmatics to prove the ability of progenitor cells to respond to IL-5 more readily. METHODS: Non-adherent non-T cells (NANT) were separated from heparinized bone marrow blood from 6 asthmatics and 3 normal subjects, loaded with CD34+ and IL-5R alpha monoclonal antibodies conjugated with immunofluorescence and then analyzed by flow cytometry. Colonies grown from progenitor cells cultured in methylcellulose were determined for 14 days in the presence or absence of ...
Abstract. The maintenance of bone marrow stromal stem cells (BMSSCs) is tightly controlled by the local microenvironment and by autocrine regulatory factors sec
One of the conventional approaches in tissue engineering is the use of scaffolds in combination with cells to obtain mechanically stable tissue constructs in vitro prior to implantation. Additive manufacturing by fused deposition modeling is a widely used technique to produce porous scaffolds with defined pore network, geometry, and therewith defined mechanical properties. Bone marrow derived mesenchymal stromal cells (MSCs) are promising candidates for tissue engineering based cell therapies due to their multipotent character. One of the hurdles to overcome when combining additive manufactured scaffolds with MSCs is the resulting heterogeneous cell distribution and limited cell proliferation capacity. In this study, we show that the use of a biaxial rotating bioreactor, after static culture of human fetal MSCs (hfMSCs) seeded on synthetic polymeric scaffolds, improved the homogeneity of cell and extracellular matrix (ECM) distribution and increased the total cell number. Furthermore, we show that the
IN their investigations of the fat composition and in vitro oxygen consumption of marrow from fed and fasted rabbits, Evans et al.1 observed a respiratory quotient of 0.85 for marrow cell suspensions incubated in the absence of glucose but in the presence of all the fatty material of whole marrow. The authors were unable to detect any uptake of fatty acid by the marrow cells and concluded that saturated fats were probably not degraded in the marrow for the production of local energy. In the work recorded here we have re-examined the question of in vitro uptake and oxidation of fatty acid by bone marrow cells. Our results indicate that fatty acid is taken up and oxidized by washed bone marrow cells suspended in a medium containing 5 per cent albumin as a carrier for fatty acid. Furthermore, we have found that glucose exerts a considerable influence on the rate of uptake and oxidation of fatty acid.
TY - JOUR. T1 - Derivation of hepatocytes from bone marrow cells in mice after radiation-induced myeloablation. AU - Theise, Neil D.. AU - Badve, Sunil. AU - Saxena, Romil. AU - Henegariu, Octavian. AU - Sell, Stewart. AU - Crawford, James M.. AU - Krause, Diane S.. PY - 2000. Y1 - 2000. N2 - Following a report of skeletal muscle regeneration from bone marrow cells, we investigated whether hepatocytes could also derive in vivo from bone marrow cells. A cohort of lethally irradiated B6D2F1 female mice received whole bone marrow transplants from age-matched male donors and were sacrificed at days 1, 3, 5, and 7 and months 2, 4, and 6 posttransplantation (n = 3 for each time point). Additionally, 2 archival female mice of the same strain who had previously been recipients of 200 male fluorescence-activated cell sorter (FACS)-sorted CD34+lin- cells were sacrificed 8 months posttransplantation under the same protocol. Fluorescence in situ hybridization (FISH) for the Y-chromosome was performed on ...
Conditioning protocols were tested for their efficacy in increasng the incidence of engraftment of histoincompatible dog bone marrow cells. Cyclophosphamide and total body irradiation (TBI), Corynebacterium parvum and TBI, a 3- or 5-day delayed transfusion of bone marrow cells after TBI, or an increase in the number of donor bone marrow cells or lymphocytes appeared to be ineffective. These protocols were previously reported to promote recovery of splenic hemopoiesis in mice in short-term assays. The noted discrepancy between studies with mice and dogs invalidated allogeneic resistance as measured in the mouse spleen assay as a model for bone marrow allograft rejection. Intravenous treatment with silica particles or L-asparaginase did improve the engraftment rate after 7.5 Gy TBI. Low efficiency and significant extra toxicity restrict the applicability of these procedures. The most promising conditioning schedule found appeared to be two fractions of 6.0 Gy TBI separated by a 72-h interval. ...
Many data indicate that statins increase mobilization of bone marrow-derived stem cells, and circulating bone marrow-derived stem cells are capable of homing to sites of myocardial infarction and endothelial disruption, thereby restoring myocardial function and microvascular integrity after acute myocardial infarction. Atorvastatin is widely used in the treatment of hyperlipidemia, especially after acute myocardial infarction. High-dose atorvastatin has been known to stop the progression of atherosclerosis and to decrease the levels of inflammatory markers.. The purpose of this prospective, randomized, single-blinded trial is to compare the effect of atorvastatin 10 mg versus 40 mg in restoring coronary flow reserve (CFR) and in serial bone marrow stem cell mobilization during the 8 months follow-up in patients with acute myocardial infarction. ...
TY - JOUR. T1 - Roles of bone marrow cells in skeletal metastases. T2 - No longer bystanders. AU - Park, Serk In. AU - Soki, Fabiana N.. AU - Mccauley, Laurie K.. PY - 2011/12. Y1 - 2011/12. N2 - Bone serves one of the most congenial metastatic microenvironments for multiple types of solid tumors, but its role in this process remains under-explored. Among many cell populations constituting the bone and bone marrow microenvironment, osteoblasts (originated from mesenchymal stem cells) and osteoclasts (originated from hematopoietic stem cells) have been the main research focus for pro-tumorigenic roles. Recently, increasing evidence further elucidates that hematopoietic lineage cells as well as stromal cells in the bone marrow mediate distinct but critical functions in tumor growth, metastasis, angiogenesis and apoptosis in the bone microenvironment. This review article summarizes the key evidence describing differential roles of bone marrow cells, including hematopoietic stem cells (HSCs), ...
A resident population of dendritic cells (DCs) has been identified in murine bone marrow, but its contribution to the regulation of hematopoiesis and establishment of the stem cell niche is largely unknown. Here, we show that murine bone marrow DCs are perivascular and have a type 2 conventional DC (cDC2) immunophenotype. RNA expression analysis of sorted bone marrow DCs showed that expression of many chemokines and chemokine receptors is distinct from that observed in splenic cDC2s, suggesting that bone marrow DCs might represent a unique DC population. A similar population of DCs was present in human bone marrow. Ablation of conventional DCs (cDCs) results in hematopoietic stem/progenitor cell (HSPC) mobilization that was greater than that seen with ablation of bone marrow macrophages, and cDC ablation also synergizes with granulocyte-colony stimulating factor to mobilize HSPCs. Ablation of cDCs was associated with an expansion of bone marrow endothelial cells and increased vascular ...
A resident population of dendritic cells (DCs) has been identified in murine bone marrow, but its contribution to the regulation of hematopoiesis and establishment of the stem cell niche is largely unknown. Here, we show that murine bone marrow DCs are perivascular and have a type 2 conventional DC (cDC2) immunophenotype. RNA expression analysis of sorted bone marrow DCs showed that expression of many chemokines and chemokine receptors is distinct from that observed in splenic cDC2s, suggesting that bone marrow DCs might represent a unique DC population. A similar population of DCs was present in human bone marrow. Ablation of conventional DCs (cDCs) results in hematopoietic stem/progenitor cell (HSPC) mobilization that was greater than that seen with ablation of bone marrow macrophages, and cDC ablation also synergizes with granulocyte-colony stimulating factor to mobilize HSPCs. Ablation of cDCs was associated with an expansion of bone marrow endothelial cells and increased vascular ...
A resident population of dendritic cells (DCs) has been identified in murine bone marrow, but its contribution to the regulation of hematopoiesis and establishment of the stem cell niche is largely unknown. Here, we show that murine bone marrow DCs are perivascular and have a type 2 conventional DC (cDC2) immunophenotype. RNA expression analysis of sorted bone marrow DCs showed that expression of many chemokines and chemokine receptors is distinct from that observed in splenic cDC2s, suggesting that bone marrow DCs might represent a unique DC population. A similar population of DCs was present in human bone marrow. Ablation of conventional DCs (cDCs) results in hematopoietic stem/progenitor cell (HSPC) mobilization that was greater than that seen with ablation of bone marrow macrophages, and cDC ablation also synergizes with granulocyte-colony stimulating factor to mobilize HSPCs. Ablation of cDCs was associated with an expansion of bone marrow endothelial cells and increased vascular ...
Minguell, J.J.; Bruzzone, M.S., 1986: Regulation of hydrocortisone binding sites by hydrocortisone in human bone marrow fibroblasts
In the present study, cytogenetic effects of Indian chrysotile asbestos in rat bone marrow cells after 290 days of intratracheal inoculation, when it develops massive pulmonary fibrosis, were investigated. The pulmonary fibrosis was confirmed by both histopathological studies and increased collagen content in the lung of the treated animals. In the asbestotic rats a significant increase in chromosomal aberrations was recorded and a decrease in mitotic index of bone marrow cells. The types of chromosomal aberrations in these cells were chromatid gaps and breaks. The results indicate the significant cytogenetic changes in the bone marrow cells of asbestotic rats and also suggest that these changes directly or indirectly may be one of the biological events involved in eliciting the asbestos-mediated toxic responses. ...
Bone marrow has been studied for a number of purposes in recent years because it is rich in stem cells - cells that can go on to become many different kinds of cells. In order to conduct this research, Isik and colleagues obtained a strain of mice whose bodies glow green under fluorescent light. The researchers removed bone marrow from the mice and then performed a stem cell transplant into a genetically identical strain of normal mice, whose cells do not glow green. Afterward, only the bone marrow of the transplanted mice glowed green inside the bodies of the mice, allowing researchers to track the bone marrow cells throughout the body. Researchers found green cells throughout the body, but observed that the highest concentration of bone marrow cells was in normal skin ...
HealthDayNews -- Stomach cancer may originate from bone marrow cells rather than stomach cells, as was previously believed. A new study in mice found that stomach cancer cells began as bone marrow cells that had migrated to the stomach. The bone marrow cells traveled to the stomach in response to inflammation caused by an infection with the bacterium that causes ulcers, Helicobacter pylori. These findings, published in the Nov. 26 issue of Science, are in stark contrast to the commonly held belief that cancers originate from the tissue in the surrounding area, meaning that it was believed that stomach cancer begins from stomach stem cells. In the last five years or so, weve learned that bone marrow-derived stem cells can go to sites of injury and mimic epithelial cells [from that region], which raised the possibility that bone marrow cells could play a role in the development of cancer in that area, said one of the studys authors, Dr. Timothy Wang, chief of the division of digestive and ...
It has been postulated that adult murine bone marrow cells have the potential to differentiate into cells of neuroectodermal origin. In order to examine whether bone marrow cells can adopt an astroglial fate, various in vivo and in vitro approaches were chosen. Lethally irradiated recipient mice were transplanted with bone marrow derived from transgenic mice which express the green fluorescent protein (GFP) under the control of the human GFAP promoter. Four weeks after transplantation, several animals underwent transient focal cerebral ischemia. Although postischemic inflammatory processes may eventually have a permissive effect on cell differentiation, not a single cells coexpressing GFAP and GFP was found in the brains of all reci-pients examined. For in vitro studies, murine bone marrow cells were co-cultured on astrocytic monolayers or organotypic entorhinal-hippocampal brain slices. Bone marrow cells were either labelled by retroviral transfection with GFP or derived from two different ...
UCLA Life Sciences Public Lecture: Bone Marrow Stem Cells: Developing New Therapies in the Fight Against Disease- Donald Kohn, January 18, 2011 Dr. Donald Kohn, UCLA professor of microbiology, immunology and molecular genetics, his research group at the Kohn Lab focus on developing new therapies for genetic diseases of the blood cells using gene therapy methods to correct hematopoietic stem cells. His laboratory performs studies on gene transfer, expression and immune response and then translates the findings into clinical trials. Read more from the original source:. Bone Marrow Stem Cells/Gene Therapy. ...
FA is a rare, inherited disease that is caused by a gene defect and that primarily affects an individuals bone marrow, resulting in decreased production of blood cells. The lack of white blood cells affects an individuals ability to fight infections, the lack of platelets may result in bleeding, and the lack of red blood cells usually leads to anemia. FA is typically diagnosed in childhood, and there is a high fatality rate. Bone marrow transplants are one common treatment for FA. However, there are many risks associated with transplantation, including rejection of the transplanted cells and graft-versus-host disease, a serious side effect in which donor cells attack the recipients tissues. This study will use an experimental gene transfer procedure performed in a laboratory to insert a new FA gene into the participants bone marrow cells. The gene-corrected bone marrow cells will then be re-infused into the participant and participants will be observed for successful gene transfer. The ...
TY - JOUR. T1 - Non-invasive in vivo molecular imaging of intra-articularly transplanted immortalized bone marrow stem cells for osteoarthritis treatment. AU - Peng, Bou-yue. AU - Chiou, Chi-Sheng. AU - Dubey, Navneet Kumar. AU - Yu, Sung Hsun. AU - Deng, Yue Hua. AU - Tsai, Feng Chou. AU - Chiang, Han Sun. AU - Shieh, Ying-Hua. AU - Chen, Wei Hong. AU - Deng, Win-Ping. PY - 2017/9/27. Y1 - 2017/9/27. N2 - Pathophysiology of osteoarthritis (OA) is characterized by progressive loss of articular cartilage in the knee-joints. To impart regenerative ability in lowly metabolizing chondrocytes, the bone marrow stem cells (BMSCs) has recently been recognized as a superior alternative treatment for OA. However, study of primary BMSCs-mediated chondrogenesis is difficult due to progressive cellular aging and replicative senescence. To obtain a therapeutic cell population for OA, BMSCs were immortalized by human papilloma virus (HPV)-16 E6/E7 along with mCherry luciferase (mCL), a gene marker for ...
Researchers also found that certain types of the stem cells were associated with the largest improvement and warrant further study.. VIDEO ALERT: Additional audio and video resources, including excerpts from an interview with Dr.Simari describing the research, are available on the Mayo Clinic News Blog.. The results were presented today at the 2012 American College of Cardiology Meeting in Chicago. They will also be published online in the Journal of the American Medical Association.. This Phase II clinical trial, designed to test this strategy to improve cardiac function, is an extension of earlier efforts in Brazil in which a smaller number of patients received fewer stem cells. For this new network study, 92 patients received a placebo or 100 million stem cells derived from the bone marrow in their hips in a one-time injection. This was the first study in humans to deliver that many bone marrow stem cells.. We found that the bone marrow cells did not have a significant impact on the original ...
Global Bone Marrow-Derived Stem Cells (BMSCS) Market By Service Type (Sample Preservation and Storage, Sample Analysis, Sample Processing, Sample Col
Fig. 4 Functional assays show increased transformation potential and sensitivity to TNK2 inhibition.. (A) Total colony formation in mouse bone marrow colony formation assay. Mouse bone marrow cells were cotransduced to express PTPN11, PTPN11 E76K, TNK2, or empty vector controls. Cells were selected for GFP+ (green fluorescent protein-positive) and puromycin resistance and plated in a methylcellulose GM-CSF sensitivity colony formation assay. Colonies were counted at 14 days [GM-CSF] = 0.05 nM (0.71 ng/ml). ****P , 0.0001 by one-way ANOVA. (B) Total colony formation in mouse bone marrow colony formation assay in cells transduced with PTPN11, PTPN11 E76K, or PTPN11 G60R. Cells were sorted for GFP+. Cells were plated with increasing concentrations of dasatinib. ***P , 0.005 and ****P , 0.0005 by one-way ANOVA. (C) Total colony formation and percent total colony formation in mouse bone marrow colony formation assay. Mouse bone marrow cells were cotransduced to express PTPN11 E76K and TNK2 or TNK2 ...
EBF2-EXPRESSING CELLS REPRESENT A HIGHLY PURIFIED MESENCHYMAL STEM CELL POPULATION IN ADULT MOUSE BONE MARROW in EXPERIMENTAL HEMATOLOGY, vol 39, issue 8, pp S109-S109 ...
Stem cells are cells that can self-renew and differentiate into a variety of cell types under certain conditions. Stem cells have great potential in regenerative medicine and cell therapy for the treatment of certain diseases. To deliver knowledge about this frontier in science and technology to medical undergraduate students, we designed an innovative practical experiment for freshmen in their second semester. The lab exercise focused on rat bone marrow mesenchymal stem cell (BMSC) isolation, cell culture and differentiation, and it aimed to help students master the aseptic techniques for cell culture, the basic methods and procedures for the primary culture and passage of BMSCs, the basic procedure for the directional differentiation of BMSCs into adipocytes and their subsequent identification by oil-red-O staining ...
The present invention relates to proteins associated with human bone marrow cell membranes for adhering hematopoietic cells to human bone marrow cell membranes. These proteins are soluble in lithium dodecyl sulfate but insoluble in 2% nonaethylene glycol octylphenol ether (e.g., 2% Triton X-100) solution. These proteins and antibodies raised against them are useful in the treatment and diagnosis of blood disorders. The DNA molecules encoding these proteins have use in gene therapy regimes. Also disclosed is a method for detecting binding between cell adhesion membrane proteins and cells having a potential to be bound to such proteins.
Image caption: Research team from Vinmec Research Institute of Stem Cell and Gene Technology in Hanoi, Vietnam.. Durham, NC - Type 2 diabetes patients who are not overweight and who have had the disorder for less than a decade can benefit from stromal stem cells transplanted from their own bone marrow, according to a study published today in STEM CELLS Translational Medicine.. In a randomized clinical trial at Vinmec Research Institute of Stem Cell and Gene Technology in Hanoi, Vietnam, researchers investigated the safety and potential therapeutic value of administering bone marrow stromal stem cells to patients with Type 2 diabetes. In each case, the cells were autologous, or taken from the patients own bodies.. A total of 30 adult patients with different body mass indexes whose Type 2 diabetes histories varied from one to 25 years were recruited for the study. Each received two infusions of the cells intravenously or by injection into an artery that supplies blood to the ...
TY - JOUR. T1 - The role of bone-marrow-derived cells in tumor growth, metastasis initiation and progression. AU - Gao, Dingcheng. AU - Mittal, Vivek. PY - 2009/8. Y1 - 2009/8. N2 - Emerging evidence from murine models suggests that tumor-specific endocrine factors systemically stimulate the quiescent bone marrow (BM) compartment, resulting in the expansion, mobilization and recruitment of BM progenitor cells. Discrete subsets of tumor-instigated BM-derived progenitor cells support tumor progression and metastasis by regulating angiogenesis, inflammation and immune suppression. Notably, clinical studies have begun to reveal that increased BM recruitment in tumors is associated with poor prognosis. Thus, the BM-derived tumor microenvironment is an attractive therapeutic target, and drugs targeting the components of the microenvironment are currently in clinical trials. Here, we focus on recent advances and emerging concepts regarding the intriguing role of BM-derived cells in tumor growth, ...
TY - JOUR. T1 - Properties of the mouse embryo conditioned medium factor(s) stimulationg colony formation by mouse bone marrow cells grown in vitro.. AU - Stanley, E. R.. AU - Bradley, T. R.. AU - Sumner, M. A.. PY - 1971/10. Y1 - 1971/10. UR - http://www.scopus.com/inward/record.url?scp=0015138971&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0015138971&partnerID=8YFLogxK. M3 - Article. C2 - 4333458. AN - SCOPUS:0015138971. VL - 78. SP - 301. EP - 317. JO - Journal of Cellular Physiology. JF - Journal of Cellular Physiology. SN - 0021-9541. IS - 2. ER - ...
Techniques for the development of ovine bone marrow-derived haemopoietic progenitor cells and in situ identification of colony morphology are described. Both mitogen stimulated lymphoid cells and antigen stimulated helper T-cells generated potent colony-stimulating activity in conditioned medium. Monocyte/macrophage, neutrophil, eosinophil, basophil/mast cell, neutrophil/monocyte and mixed phenotype colonies developed in stimulated bone marrow cultures in a conditioned medium dose-dependent manner. Neutrophil, monocyte/macrophage and eosinophil colonies were detected in greater numbers than the other types, with mixed colonies representing only around 1% of the total. Eosinophil colonies were particularly abundant when compared to published reports of the numbers obtained with similar cultures of normal mouse or human bone marrow cells. This culture technique will allow a detailed analysis of both ovine colony-stimulating factors and of the distribution of haemopoietic progenitor cells in vivo.
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It is important to weigh both the risks and the benefits of a bone marrow/stem cell transplant. A transplant doctor can answer your questions and help you decide if a transplant is an option for you. A bone marrow transplant has serious risks. Some patients suffer from life-threatening problems as a result of their transplant. These problems can include serious infections and graft-versus-host disease (GVHD), in which the transplanted cells attack the patients body.
It is important to weigh both the risks and the benefits of a bone marrow/stem cell transplant. A transplant doctor can answer your questions and help you decide if a transplant is an option for you. A bone marrow transplant has serious risks. Some patients suffer from life-threatening problems as a result of their transplant. These problems can include serious infections and graft-versus-host disease (GVHD), in which the transplanted cells attack the patients body.
BACKGROUND & AIMS: Myofibroblasts of bone marrow origin have recently been found in a number of parenchymal organs such as the gut and kidney. We have analyzed the origin of myofibroblasts within fibrotic liver in 2 scenarios: (1) 7 male patients (hepatitis B; hepatitis B and D; Wilsons disease; hepatitis B, D, and C; and 3 with hepatitis C) who received liver transplants from female donors and subsequently developed liver fibrosis and (2) a female patient who received a bone marrow transplant from a male donor and subsequently developed hepatitis C-induced cirrhosis. METHODS: Through the use of in situ hybridization for the Y chromosome, we have tracked male cells of extrahepatic origin. The phenotype of these male cells was examined by immunohistochemistry using a panel of antibodies against alpha-smooth muscle actin (alpha SMA), vimentin, fibulin-2, and leukocyte common antigen (CD45). Confocal microscopy was performed to confirm the location of the Y chromosome probe within the ...
Delayed spontaneous apoptosis in immature bone marrow neutrophils compared with mature blood neutrophils. (A) Viability assay in the absence of survival and dea
Developmental biologist Lorraine Iacovitti, Ph.D., associate director of the Farber Institute for Neurosciences at Thomas Jefferson University in Philadelphia and her co-workers had previously shown that by using a potion of growth factors and other nutrients in the laboratory, they were able to convert adult human bone marrow stem cells into adult brain cells. Human adult bone marrow stem cells - also known as pluripotent stem cells - normally give rise to human bone, muscle, cartilage and fat cells ...
Gene targeting experiments have demonstrated that the transcription factor SCL is essential for primitive and definitive hematopoiesis in the mouse. To study the functional properties of hematopoietic cells expressing SCL, we have generated mutant mice (SCLlacZ/w) in which the Escherichia coli lacZ reporter gene has been knocked in to the SCL locus, thereby linking beta-galactosidase expression to transcription from the SCL promoter. Bone marrow cells from heterozygous SCLlacZ/w mice were sorted into fractions expressing high, intermediate and low levels of beta-galactosidase (designated lacZhigh, lacZint, and lacZneg). Cells that were lacZhigh or lacZint were enriched for day 12 spleen colony-forming units and myeloid and erythroid colony-forming cells (CFCs). These fractions included ,99% of the erythroid and ,90% of the myeloid CFCs. Culture of sorted bone marrow populations on stromal cells secreting interleukin-7 or in fetal thymic organ cultures showed that B and T lymphoid progenitors ...
Kiesche, Amy, H-2 associated natural resistance to normal bone marrow cells. (1983). Summer and Academic Year Student Reports. 754 ...
The addition of bone marrow cells or peripheral lymphocytes to the isolated pig spleen markedly enhanced the primary antibody response after 3-day perfusion and antigenic challenge in vitro. The splenic preparation without added cells or with the addition of marrow cells to an irradiated spleen gave a limited response. Contributory evidence is provided that at least two distinct cell types are needed for antibody production. For optimal antibody response by an isolated perfused spleen, marrow cells or peripheral lymphocytes should be added to the system.. ...
A method is described for generating a clinically significant volume of neural progenitor cells from whole bone marrow. A mass of bone marrow cells may be grown in a culture supplemented with fibroblast growth factor-2 (FGF-2) and epidermal growth factor (EGF). Further methods of the present invention are directed to utilizing the neural progenitor cells cultured in this fashion in the treatment of various neuropathological conditions, and in targeting delivery of cells transfected with a particular gene to diseased or damaged tissue.
A meta-analysis by Lipinski et al. (5) included 10 of these trials (7 randomized, 3 cohort studies) on intracoronary cell injection (within the first 14 days after infarction), yielding 698 patients, of which 659 were available at follow-up (median follow-up of 6 months). In 2 trials (n = 126), peripheral blood cells were used for intracoronary infusion and in 8 investigations bone marrow-derived cells were used. For the pooled population, Lipinski et al. (5) found a significantly superior improvement in LVEF of 3.0% (95% confidence interval: 1.9% to 4.1%, p , 0.00001) for subjects receiving bone marrow transplantation in comparison with control subjects. Similarly, LV end-systolic volumes were reduced in patients receiving cell therapy by -7.4 ml (95% confidence interval: -12.2 to -2.7 ml, p , 0.002) compared with control subjects. Changes in end-diastolic volumes were not significantly different between groups in this meta-analysis.. Clinical end points, such as death, target vessel ...
AppliedStemCell eCommerce Platform Human Bone Marrow Mononuclear Cells (DCM) [ASE-5071] - Catalog Number ASE-5071; ASE-5072; ASE-5073 Quantity 2.5 x 106 viable cells/mL; 10.0 x 106 viable cells/mL; 25.0 x 106 viable cells/mL Product Information Descriptio
Human Bone Marrow Mononuclear Cells are approximately 15 to 25 μm in diameter. Unfortunately I dont have any internal protocols for injecting mice. 27G may lyse the cells. So you may need to use 25 G needle. Prior to injecting the mice, I would recommend checking cell viability after passing the cells through the gauge needle you intend to use. You may be able to find some protocols online though.. ...
Katie Kraushaar opens a core shop bone marrow stem cell therapy for ErrorDocument vertebrae & at Hixson Middle School in St. She is to complete the schedule of having in her general by stay-connected-to-everything a sure hair with the pattern pp. and by submitting wide with her copies. In j to her seven books in the l, Katie is a Teacher Consultant for the Gateway Writing Project, a contrast of the National Writing Project. including Out the Welcome; Wagon! retailers requested wanting on, I partnered refreshing for students! I abnormally serve the something of Teaching g and Surface. I will Moreover Provide this hand with names. Katie, I stand how pulmonary you require not stopping invalid questions are incredible and services have to save! I know the aspect is a related employer and I are to work it. I not were your administrator and can please how genuine you assign not hanging format! so prosocial to call it failed Quarterly! accurate no an magical file taht! I expose up-to-the-minute ...
Helen Pearson. Prof. Catherine Verfaillie has isolated a stem cell from adult human bone marrow that can produce all tissue types.. June 21, 2002; US scientists have reversed the symptoms of Parkinsons Disease in rats using stem cells from mouse embryos [1]. Another team has compelling evidence that they have isolated a stem cell from adult human bone marrow that can produce all the tissue types in the body, from blood to muscle to nerve [2]. Stem cells from embryos were known to give rise to every type of cell. Those from adults were previously thought to have a more limited repertoire.. Researchers hope to use stem cells to repair or replace diseased or damaged organs, leading to new treatments for human disorders that are currently incurable, including diabetes, spinal-cord injury and brain diseases. The new reports may re-fuel the debate in the US Senate over whether to permit the cloning of human embryos for medical research, which stalled earlier this week. US scientists are fighting to ...
TY - JOUR. T1 - TGF beta 1 limits the expansion of the osteoprogenitor fraction in cultures of human bone marrow stromal cells. AU - Walsh, S.. AU - Jefferiss, C.. AU - Stewart, K.. AU - Beresford, J. N.. N1 - ID number: ISI:000181664900006. PY - 2003. Y1 - 2003. N2 - Currently, there is considerable interest in the possibility of using cultured human bone marrow stromal cells (BMSCs) for skeletal tissue engineering. However, the factors that regulate their ex vivo expansion and promote their osteogenic maturation remain poorly defined. Using BMSCs obtained from a large cohort of adult donors, the effects of transforming growth factor (TGF)beta1 on these processes have been determined. BMSCs were found to express TGFbeta receptors (TbetaRs) I, II, III (betaglycan) and CD105/endoglin. The expression of TbetaRs I and II, but not TbetaR III or endoglin, was linked to the cells state of maturation. Treatment with TGFbeta increased the colony-forming efficiency (CFE) of marrow cell suspensions but ...
Title: Involvement of Adipogenic Potential of Human Bone Marrow Mesenchymal Stem Cells (MSCs) in Osteoporosis. VOLUME: 3 ISSUE: 3. Author(s):J. Pablo Rodriguez, Pablo Astudillo, Susana Rios and Ana Maria Pino. Affiliation:Laboratorio de Biologia Celular. INTA, Universidad de Chile, Macul 5540, Macul. Casilla 138-11, Santiago, Chile.. Keywords:Osteoporosis, mesenchymal stem cells, adipogenesis, osteogenesis, PPARγ, leptin, estrogens. Abstract: Mesenchymal Stem Cells (MSCs) from bone marrow stroma are capable of differentiating into osteoblasts and adipocytes, among other cell phenotypes. In normal bone marrow osteoblastic and adipocytic cell differentiation occur in favor of bone formation, but this relationship appears disrupted in several bone diseases. In osteoporosis increased bone marrow adipocyte production is counterbalanced by diminished production of osteogenic cells. Since osteoblats and adipocytes originate from a common MSC precursor cell, quantitative and qualitative stem cell ...
With the expression bone marrow hematopoietic stem cell transplant we intend a complex procedure used especially, but not only, in the treatment of leukimias and lymphomas. Stem cells can be obtained not only from bone marrow but also from peripheral blood after a specific preconditioning of the patient, or from umbilical-cord blood.. Indications for hematopoietic stem cell transplant are acute leukimias, chronic leukimias, different forms of bone marrow insufficiency, thalassemias, Hodgkin lymphoma, non Hodgkin lymphomas, myelomas, other chronic myeloproliferative diseases, numerous genetic disorders and, as a recent indication, some autoimmune illnesses.. ...
TY - JOUR. T1 - Intracerebral Xenotransplantation of GFP Mouse Bone Marrow Stromal Cells in Intact and Stroke Rat Brain. T2 - Graft Survival and Immunologic Response. AU - Irons, H.. AU - Lind, J. G.. AU - Wakade, Chandramohan G.. AU - Yu, G.. AU - Hadman, M.. AU - Carroll, James Edwin. AU - Hess, David C. AU - Borlongan, Cesar V.. PY - 2004/1/1. Y1 - 2004/1/1. N2 - The present study characterized survival and immunologic response of bone marrow stromal cells (BMSCs) following transplantation into intact and stroke brains. In the first study, intrastriatal transplantation of BMSC (60,000 in 3 μl) or vehicle was performed in normal adult Sprague-Dawley male rats that subsequently received daily cyclosporin A (CsA, 10 mg/kg, IP in 3 ml) or vehicle (olive oil, similar volume) starting on day of surgery up to 3 days posttransplantation. Animals were euthanized at 3 or 30 days posttransplantation and brains were processed either for green fluorescent protein (GFP) microscopy or flow cytometry ...
Dive into the research topics of Bioreactor Expansion of Human Adult Bone Marrow-Derived Mesenchymal Stem Cells. Together they form a unique fingerprint. ...
TY - JOUR. T1 - Redifferentiation of dedifferentiated chondrocytes and chondrogenesis of human bone marrow stromal cells via chondrosphere formation with expression profiling by large-scale cDNA analysis. AU - Imabayashi, Hideaki. AU - Mori, Taisuke. AU - Gojo, Satoshi. AU - Kiyono, Tohru. AU - Sugiyama, Tomoyasu. AU - Irie, Ryotaro. AU - Isogai, Takao. AU - Hata, Jun Ichi. AU - Toyama, Yoshiaki. AU - Umezawa, Akihiro. PY - 2003/8/1. Y1 - 2003/8/1. N2 - Characterization of dedifferentiated chondrocytes (DECs) and mesenchymal stem cells capable of differentiating into chondrocytes is of biological and clinical interest. We isolated DECs and bone marrow stromal cells (BMSCs), H4-1 and H3-4, and demonstrated that the cells started to produce extracellular matrices, such as type II collagen and aggrecan, at an early stage of chondrosphere formation. Furthermore, cDNA sequencing of cDNA libraries constricted by the oligocapping method was performed to analyze difference in mRNA expression profiling ...
BioAssay record AID 44634 submitted by ChEMBL: HSF produced by bone marrow-derived stromal cell lines C6.4 on stimulation with the compound at (1000 ng/mL) was determined in vitro in an GM-CFC assay..
Horowitz MC, Berry R, Holtrup B, Sebo Z, Nelson T, Fretz JA, Lindskog D, Kaplan JL, Ables GP, Rodeheffer MS, Rosen CJ.. Adipocyte. 2017 Jul 3;6(3):193-204.. PMID: 28872979. Adipocytes were identified in human bone marrow more than a century ago, yet until recently little has been known about their origin, development, function or interactions with other cells in the bone marrow. Little functional significance has been attributed to these cells, a paradigm that still persists today. However, we now know that marrow adipose tissue increases with age and in response to a variety of physiologic induction signals. Bone marrow adipocytes have recently been shown to influence other cell populations within the marrow and can affect whole body metabolism by the secretion of a defined set of adipokines. Recent research shows that marrow adipocytes are distinct from white, brown and beige adipocytes, indicating that the bone marrow is a distinct adipose depot. This review will highlight recent data ...
Tumor tropism of human bone marrow-derived mesenchymal stem cells (MSC) has been exploited for the delivery of therapeutic genes for anticancer therapy. However, the exact contribution of these cells in the tumor microenvironment remains unknown. In this study, we examined the biological effect of MSC on tumor cells. The results showed that MSC inhibited the growth of human glioma cell lines and patient-derived primary glioma cells in vitro. Coadministration of MSC and glioma cells resulted in significant reduction in tumor volume and vascular density, which was not observed when glioma was injected with immortalized normal human astrocytes. Using endothelial progenitor cells (EPC) from healthy donors and HUVEC endothelial cells, the extent of EPC recruitment and capacity to form endothelial tubes was significantly impaired in conditioned media derived from MSC/glioma coculture, suggesting that MSC suppressed tumor angiogenesis through the release of antiangiogenic factors. Further studies using ...
Cytotoxic T-lymphocyte-associated protein 4- (CTLA4-) modified human bone marrow-derived mesenchymal stem cells (hBMMSCs) might be promising seed cells for bone tissue engineering. However, the underlying mechanism is not clear. In the present study, we investigated whether CTLA4-modified hBMMSCs are involved in the migration of allogeneic hBMMSCs (allo-hBMMSCs) by maintaining POSTN secretion. hBMMSCs were isolated from different groups, named hBMMSCs and allo-hBMMSCs. hBMMSCs that were infected with the negative control (NC), empty adenovirus- or recombinant adenovirus-expressing CTLA4, POSTN, or CTLA4 plus the shRNA of POSTN were named NC hBMMSCs, CTLA4-modified hBMMSCs, POSTN-modified hBMMSCs, or CTLA4+shPOSTN-modified hBMMSCs, respectively. They were then cocultured with PBMCs in a 1 : 5 ratio with 2.5 |i|μ|/i|g/mL phytohemagglutinin (PHA). The coculture supernatant was collected to treat allo-hBMMSCs with anti-integrin |i|α|/i|v|i|β|/i|3 IgG, or negative
Objectives: Human bone marrow stromal cells (hBMSCs) are adherent fibroblast-like cells found in the bone marrow. They are a heterogeneous population of cells that includes a subset of osteoprogenitors. BMSCs have been widely used for tissue engineering, especially for bone regeneration. However, for clinical application currently, large quantities of hBMSCs are usually required for transplantation which is typically produced by serial passages of the cells ex vivo. We examined the effects of in vitro expansion on hBMSCs proliferation, multidifferentiation, and gene expression profiles. Methods: hBMSCs were harvested from surgical waste bone specimens from 3 healthy adults with IRB approval. The hBMSCs were cultured in α-MEM with 10% FBS and 1% penicillin-streptomycin. hBMSCs were trypsinized and passaged when they reached 70-80% confluence. Cells from early passage (p2 or 3) were compared with late passage (p7 or 8). MTT assay was used to determine the growth kinetics of hBMSCs. ...
Hepatitis B virus (HBV) infection is a blood borne infectious disease that affects the liver. Human bone marrow mesenchymal stem cells (BMSCs) may serve as a cell source for adult stem cell transplantation in liver repair. However, the susceptibility of human BMSCs to HBV infection is poorly understood. The aim of this study was to investigate the infection and replication of HBV in cultures of human BMSCs. Human BMSCs were confirmed using flow cytometry. Intracellular HBV DNA was detected at d 2 after infection and maintained at relatively high levels from d 6 to d 12. The maximal level of intracellular HBV DNA was 9.37 × 105 copies/mL. The extracellular HBV DNA was observed from d 3 to d 15, and the levels ranged from 3.792 × 102 copies/mL to 4.067 × 105 copies/mL. HBsAg in the culture medium was detected from d 2 to d 16. HBeAg secretion was positive from d 5 to d 13. HBcAg constantly showed positive signals in approximately 7%-20% of BMSCs from 2 days after exposure. Intracellular HBV covalently
Cheapest Bone Marrow Stem Cell Therapies price in Bulgaria is $. Average Bone Marrow Stem Cell Therapies cost $0, where prices can go as high as $. PlacidWay Medical Tourism provides cost comparison for Bone Marrow Stem Cell Therapies, Stem Cell Therapy Prices in Bulgaria. Explore Bone Marrow Stem Cell Therapies prices worldwide.
HemoGenyx is a preclinical-stage biotechnology company focused on the discovery, development and commercialisation of novel therapies and treatments for blood diseases, like leukemia and lymphoma. The companys leading technologies aim to change the way in which bone marrow/hematopoietic stem cell (BM/HSC) transplants are performed and improve their efficacy.
Fingerprint Dive into the research topics of Adipocytes derived from human bone marrow mesenchymal stem cells exert inhibitory effects on osteoblastogenesis. Together they form a unique fingerprint. ...
TY - JOUR. T1 - In vivo reactivity of mouse natural killer (NK) cells against normal bone marrow cells. AU - Riccardi, C.. AU - Santoni, A.. AU - Barlozzari, T.. AU - Herberman, R. B.. PY - 1981/5/1. Y1 - 1981/5/1. N2 - An in vivo role for mouse natural killer (NK) cells in the rapid rejection of transplantable tumors has been previously demonstrated, using an assay of elimination of [125I]iododeoxyuridine-labeled tumor cells from the lungs and other organs. We have now used the same technique to examine the role of NK cells in in vivo clearance of syngeneic or allogeneic bone marrow cells from normal mice. The degree of clearance from the lungs or liver, at 4 hr after intravenous inoculation of radiolabeled bone marrow cells, correlated with the levels of NK activity in the recipients. Young CBA mice, with high NK activity, showed substantially more clearance of bone marrow cells than SJL mice, with low NK activity. Within the same strain, mice at 7 weeks of age had higher in vivo as well as in ...
1α,25-Dihydroxyvitamin D3 [1,25(OH)2D3] is known to modulate Ca2+ metabolism in several cell types. Vitamin-D-dependent calcium binding proteins such as calbindin-D28K (28 kDa calcium binding proteins) have been shown to be regulated by 1,25(OH)2D3 but the mechanisms controlling calbindin synthesis are still poorly understood in human osteoblast cell culture models. The human bone marrow stromal cells (HBMSC) described in this paper developed a calcified matrix, expressed osteocalcin (OC), osteopontin (OP) and responded to 1,25(OH)2D3. The expression of vitamin D receptor mRNA was demonstrated by reverse transcription-PCR. Calbindin-D28K protein was identified only in cells arising from the sixth subculture, which exhibited a calcified matrix and all of the osteoblastic markers, e.g. OC and OP. It was demonstrated by dot-immunodetection using immunological probes, and by in situ hybridization using labelled cDNA probes. Moreover, vitamin D3 enhanced calbindin-D28K synthesis as well as OC ...
Connexin43 (Cx43) is a component of gap junctions and is involved in intercel- lular signaling following injury to tissues. The carboxyl terminus of Cx43 binds to the PDZ2 domain of ZO-1 in order to form gap junction plaques and connect to the cytoskeleton. A biomimetic peptide known as αCT-1, replicating the last 9 amino acids found in the carboxyl terminus of CX43, has been shown to improve wound healing by preferentially binding to the PDZ2 domain of ZO-1. A possible mecha- nism for its action is through the Epithelial-Mesenchymal Transformation (EMT). Scratch assays were performed on rat bone marrow stromal cells treated with the peptide and were then analyzed using qPCR, western blotting, confocal microscopy, and live cell imaging. The gene expression analysis showed up-regulation of F11r and Krt19 and down-regulation of Mmp3. Protein expression analysis indicated an increase in Krt19 and the complete absence of Snai2 in the αCT-1 treated samples. Confocal microscopy suggested increased actin
A predominant challenge in developing curative leukemia therapy is interactions of leukemic cells with the bone marrow stromal microenvironment. We aimed to investigate the role of stromal cells, such as bone marrow mesenchymal stromal cells (BMSCs) and osteoblasts (OBs), in curcumin (CUR) and daunorubicin (DNR) induced apoptosis of acute myeloid leukemia (AML) cells. We used KG1 and U937 as leukemia cell line models and treated them with CUR and DNR. The cells were then co-cultured with BMSCs or a combination of BMSCs and OBs as feeders. After 24 hours of co-culture, BMSCs or OBs were sorted and separated from the leukemia cells and apoptosis levels were analyzed by annexin/propidium iodide (PI) staining on flow cytometry. Potentially involved molecular pathways were analyzed at gene and protein levels by Real time PCR and western blotting, respectively. The results showed AML cells co-cultured with BMSCs plus OBs to be more resistant to drug induced-apoptosis compared to co-culture with BMSCs alone
Bone marrow stromal cells protect hematopoietic cells and provide drug resistance by delivering bunch of variable proteins. Thus, alterations of protein expression are typically associated with cell-cell signal transduction and regulation of cellular functions. Co-culture models of bone marrow stromal cells and hematopoietic cells are often used in studies of their crosstalk. Studies of altered protein expression initiated by stromal cell/hematopoietic cell interactions are an important new trend in microenvironmental research. There has been no report to date of global quantitative proteomics analysis of crosstalk between hematopoietic cells and stromal cells. In this study, we analyzed quantitative proteomes in a co-culture system of stromal HS5 cells and hematopoietic KG1a cells, and simultaneously tracked differentially expressed proteins in two types of cells before and after co-culture by stable isotope labeling by amino acids in cell culture (SILAC) method. We have shown that in co-cultured KG1a,
DOI: 10.11607/jomi.te56 Purpose: This study investigated the role of the bone marrow derived CD34+ cell in a milieu of osteoprogenitor cells, bone marrow plasma cell adhesion molecules, recombinant human bone morphogenetic protein (rhBMP), and a matrix of crushed cancellous allogeneic bone in the clinical regeneration of functionally useful bone in craniomandibular reconstructions. The history and current concepts of bone marrow hematopoietic stem cells and mesenchymal stem cells are reviewed as they relate to bone regeneration in large continuity defects of the mandible. Materials and Methods: Patients with 6- to 8-cm continuity defects of the mandible with retained proximal and distal segments were randomized into two groups. Group A received an in situ tissue-engineered graft containing 54 38 CD34+ cells/mL along with 54 38 CD44+, CD90+, and CD105+ cells/mL together with rhBMP-2 in an absorbable collagen sponge (1 mg/cm of defect) and crushed cancellous allogeneic bone. Group B received the ...
Here, we demonstrate that bone marrow-derived stem cells engraft the murine endometrium. Both stromal and epithelial cells were derived from bone marrow origin. These data show the potential for stem cells to have a role in the regeneration or repair of this tissue after injury. However, the small number of engrafted cells limits their potential to significantly contribute to cyclic endometrial function during each estrus cycle. In other organs, the homing and engraftment of stem cells are influenced by injury and inflammation, presumably through the generation of a signal emanating from the damaged tissue [17, [18]-19]. A more significant engraftment of endometrium by bone marrow is likely to occur after endometrial injury or inflammatory insult. Additionally, the proliferation and development of endometrium are entirely regulated by hormonal stimuli. Ovarian estrogen and progesterone drive endometrial growth and apoptosis [20, 21]. As the radiation used prior to bone marrow transplantation ...
Bone marrow aspirate concentrate (BMA/BMAC) is perhaps the most popular stem cell injection available today. The treatment involves injecting the patients own bone marrow cells into the injured area with the hope that it will help regrow tissue or reduce inflammation. The procedure involves two steps: (1) patients are lightly anesthetized and bone marrow is removed through a needle from the bone, most commonly the iliac crest of the patients hip, and then (2) the bone marrow is processed to concentrate the cells. After the bone marrow has been centrifuged (spun in a circle to separate the various blood contents), the BMA/BMAC is put into a syringe and injected into the affected body part.. Bone marrow contains progenitor cells, which are cells that have been primed to become specific cells. A small fraction of these cells (about 1% of the total cells in bone marrow) are mesenchymal stem cells, which can become cartilage, bone, and tendon cells. It is thought that these mesenchymal ...
In this study, the role of histamine in interleukin-1 (IL-1) formation in murine bone marrow stromal cells was investigated in vitro. It was found that histamine and 4-methylhistamine increased the number of granulocyte colony-forming units in murine bone marrow cells. A similar effect was elicited by dibutyryl-cAMP and theophylline. When histamine and H2 agonists, such as 4-methylhistamine and dimaprit, were added to the culture medium containing murine bone marrow stromal cells, thymocyte comitogenic activity detected in the medium increased significantly. However, no such effect was observed in the case of 2-methyl-histamine, an H1 agonist. Histamine-induced production of thymocyte comitogenic activity in bone marrow stromal cells was inhibited by some H2 antagonists, such as cimetidine, ranitidine, and famotidine, but not by the H1 antagonist pyrilamine. Histamine was also effective in inducing the colony-promoting activity in murine bone marrow stromal cells. This was also inhibited by H2 ...
Bone marrow stromal cells (BMSCs) are multipotent cells that support angiogenesis, wound healing, and immunomodulation. In the hematopoietic niche, they nurture hematopoietic cells, leukemia, tumors and metastasis. BMSCs secrete of a wide range of cytokines, growth factors and matrix proteins which contribute to the pro-tumorigenic marrow microenvironment. The inflammatory cytokines IFN-γ and TNF-α change the BMSC secretome and we hypothesized that factors produced by tumors or leukemia would also affect the BMSC secretome and investigated the interaction of leukemia cells with BMSCs. BMSCs from healthy subjects were co-cultured with three myeloid leukemia cell lines (TF-1, TF-1α and K562) using a trans-well system. Following co-culture, the BMSCs and leukemia cells were analyzed by global gene expression analysis and culture supernatants were analyzed for protein expression. As a control, CD34+ cells were also cocultured with BMSCs. Co-culture induced leukemia cell gene expression changes in stem
Introduction: Conventionally cultured mouse bone marrow mesenchymal stromal cells (mBM-MSC) are a heterogeneous population that often initially contain contaminating haematopoietic cells. Variability in isolation methods, culture protocols and the lack of specific mBM MSC markers might explain this heterogeneity. The aim of this study is to optimise the isolation, culture conditions and selection of mBM-MSC. Methods: Mouse BM-MSCs were isolated from crushed long bones (cBM) or flushed bone marrow (fBM) from 6-8 week old C57Bl/6 mice. These subpopulations were analysed by flow cytometry using commonly used mBM-MSC cell surface marker, e.g. Sca-1, CD29 and CD44. Cells were cultured and expanded in vitro in hypoxic conditions of either 2 % or 5 % oxygen. Cell sorting and qRT-PCR was used to determine transcript levels of stem cell and lineage related genes in individual subpopulations. Results: During early passaging not only do contaminating haematopoietic cells disappear, but there is a change in ...
TY - JOUR. T1 - Rapid 1-hour transduction of whole bone marrow leads to long-term repopulation of murine recipients with lentivirus-modified hematopoietic stem cells. AU - Kurre, Peter. AU - Anandakumar, P.. AU - Kiem, H. P.. PY - 2006/2. Y1 - 2006/2. N2 - Efficient gene transfer to hematopoietic stem cells by Moloney murine leukemia virus-derived retroviral vectors benefits from ex vivo culture and cytokine support. Both also increase the risks of apoptosis and differentiation among cells targeted for transduction. In an effort to maximize the retention of stem cell properties in target cells, we developed a transduction protocol with a focus on minimizing graft manipulation, cytokine stimulation, and ex vivo exposure duration. Based on their wide host range and ability to transduce quiescent cells, human immunodeficiency virus (HIV)-derived lentivirus vectors are ideally suited for this purpose. Our present studies in a murine model show that whole bone marrow cells are readily transduced ...
The bone marrow plays a unique role within the immune system. We compared the phenotype and function of virus-specific CD8(+) T cells from matched samples of human peripheral blood and bone marrow. Analysis of virus-specific memory CD8(+) T cells showed widely divergent partition of antigen-specific populations between blood and bone marrow. T cells specific for Epstein-Barr virus (EBV) lytic antigens were enriched 3-fold in marrow compared with blood, whereas the response to EBV latent epitopes was equivalent between the 2 compartments. No difference in EBV viral load or expression of the EBV lytic protein was observed between blood and bone marrow. In direct contrast, although cytomegalo-virus (CMV)-specific T cells were the largest virus-specific population within peripheral blood, they were reduced by 60% within marrow. Bone marrow T cells were found to exhibit a unique CCR5(+)CXCR6(+)CXCR3(-) homing phenotype which has not been observed on T cells from other secondary lymphoid organs or peripheral
Introduction Recent evidence has shown that bone marrow cells play critical roles during the inflammatory proliferative and remodeling phases of cutaneous wound healing. assays. The preparations examined were whole bone marrow (WBM) whole bone marrow (long term initiating/hematopoietic based) cultured cells (BMC) and bone marrow derived mesenchymal stem cells (BM-MSC). We also applied these bone marrow preparations in two murine models of radiation induced delayed wound healing to determine which had a greater effect on healing. Results Angiogenesis assays demonstrated that tube formation was stimulated by both WBM and BMC with WBM having the greatest effect. Scratch wound assays showed higher fibroblast migration at 24 48 and 72 hours in presence of WBM as compared to BM-MSC. WBM also appeared to stimulate a greater healing response than BMC and BM-MSC in a radiation induced delayed wound healing animal model. Conclusions These studies promise to help elucidate the role of stem cells during ...
bone-marrow translation in English-Spanish dictionary. bone marrow is an alternate term for marrow. Use DeepL Translator to instantly translate texts and documents. Translate bone marrow into Spanish. Report an error or suggest an improvement. Alternative forms . This page provides all possible translations of the word yellow bone marrow in the Spanish language. El orador fue directo al centro de su argumento. Lo encontrarás en al menos una de las líneas abajo. His topic was the regeneration of damaged heart muscle, by use of bone marrow stem cells. Contextual translation of bone marrow into Spanish. bone marrow (usually uncountable, plural bone marrows) The fatty vascular tissue that fills the cavities of bones, being the place where new blood cells are produced. Bone marrow depression Bone marrow depression, usually presenting as granulocytopenia or agranulocytosis, has been reported during treatment with Remeron. Chipre cuenta con uno de los mayores bancos, After the skin is ...
1. Bone marrow is commonly collected and examined when abnormalities are found in the circulating blood. The most common abnormality is a persistent shortage of one of the blood cell types. This is a serious situation and may be due to a problem in the bone marrow. Examination of marrow can often provide information about the underlying cause, and may help to predict the outcome.. 2. Bone marrow is also collected and examined to look for certain types of cancer. Some cancers start right in the cells of the bone marrow and other cancers spread to the bone marrow from elsewhere in the body. Cancer that starts in the bone marrow is sometimes called leukemia. Examination of the bone marrow helps to identify the cancer, and reveals how seriously the marrow is affected.. 3. Occasionally, bone marrow is collected and examined to investigate other problems such as persistent fever, unexplained weight loss, high blood calcium levels (see article on Hypercalcemia), and high serum protein level (see ...
Purpose: : To determine if bone marrow-derived stem cells (BMSC) have the capacity in vitro and in vivo to express retinal pigment epithelial (RPE)-like markers. Methods: : In vitro, mouse Sca-1+ GFP+ cells of bone marrow origin were used in coculture with adult mouse RPE cells. The coculture in a 1:1 ratio was performed with and without cell-cell-contact for up to 3 weeks. Mouse fibroblasts served as a control. Immunocytochemical analysis was performed using monoclonal antibodies (mAbs) against specific RPE markers - cytokeratin, RPE65, MITF - as well as non-RPE markers - opsin (photoreceptors) and glial fibrillary acidic protein (GFAP; glia). In vivo, sodium iodate (NaIO3) was used to damage the RPE. For this study, C57BL/6 mice were injected i.v. with 35 mg/kg NaIO3 followed by the subretinal (s.r.) injection of 3x104 Sca-1+ GFP+ BMSC on day 3. The mice were sacrificed on days 7, 14, 21, and 28 after transplantation. Whole eye flat mounts (FM) were prepared and examined for GFP+ cells under a ...
The two types of bone marrow are red marrow (Latin: medulla ossium rubra), which consists mainly of hematopoietic tissue, and yellow marrow (Latin: medulla ossium flava), which is mainly made up of fat cells. Red blood cells, platelets, and most white blood cells arise in red marrow. Both types of bone marrow contain numerous blood vessels and capillaries. At birth, all bone marrow is red. With age, more and more of it is converted to the yellow type; only around half of adult bone marrow is red. Red marrow is found mainly in the flat bones, such as the pelvis, sternum, cranium, ribs, vertebrae and scapulae, and in the cancellous (spongy) material at the epiphyseal ends of long bones such as the femur and humerus. Yellow marrow is found in the medullary cavity, the hollow interior of the middle portion of short bones. In cases of severe blood loss, the body can convert yellow marrow back to red marrow to increase blood cell production.. ...
Objectives: To investigate whether human bone marrow (BM) derived mesenchymal stem cells (MSC) and articular chondrocytes (AC) affect the in vitro proliferation of T-lymphocytes and peripheral blood mononuclear cells (PBMC) driven by the homeostatic IL 2, IL 7 and IL 15 cytokines binding to the common cytokine receptor γ-chain (γc ) in the absence of T-cell receptor (TCR) triggering.. Methods: PBMCs, total-T cells and T cell subsets (CD4+ and CD8+) were stimulated with IL 2, IL 7 or IL 15 and exposed to cultured BM-MSCs and ACs at varying cell:cell ratio either in contact or in transwell conditions. Lymphocyte proliferation was measured by 3H-thymidine uptake or by flow cytometry on CFSE labelled lymphocytes.. Results Both MSCs and ACs enhanced and inhibited lymphocyte proliferation depending on the extent of lymphocyte baseline proliferation and on the MSC/AC to lymphocyte ratio. Enhancement was significant on poorly proliferating lymphocytes and mostly at lower MSC/ AC to lymphocyte ratio. ...
In vitro osteogenic potential of human bone marrow stromal cells cultivated in porous scaffolds from mineralized collagen.: Porous 3D structures from mineralize
TY - JOUR. T1 - Heparan sulfate enhances the self-renewal and therapeutic potential of mesenchymal stem cells from human adult bone marrow. AU - Helledie, Torben. AU - Dombrowski, Christian. AU - Rai, Bina. AU - Lim, Zophia X.H.. AU - Hin, Ian Lee Hock. AU - Rider, David A.. AU - Stein, Gary S.. AU - Hong, Wanjin. AU - Van Wijnen, Andre J.. AU - Hui, James H.. AU - Nurcombe, Victor. AU - Cool, Simon M.. PY - 2012/7/20. Y1 - 2012/7/20. N2 - Insufficient cell number hampers therapies utilizing adult human mesenchymal stem cells (hMSCs) and current ex vivo expansion strategies lead to a loss of multipotentiality. Here we show that supplementation with an embryonic form of heparan sulfate (HS-2) can both increase the initial recovery of hMSCs from bone marrow aspirates and increase their ex vivo expansion by up to 13-fold. HS-2 acts to amplify a subpopulation of hMSCs harboring longer telomeres and increased expression of the MSC surface marker stromal precursor antigen-1. Gene expression profiling ...
Bone marrow is the soft spongy tissue that lies within the hollow interior of long bones. In adults, marrow in large bones produces new blood cells. Bone marrow forms around 4% of total body weight. There are two types of bone marrow: red marrow that is responsible for producing red blood cells, white blood cells and platelets; and yellow marrow consisting mainly of fat cells.. International Journal of Bone Marrow Research publishes rigorously peer-reviewed manuscripts focusing on latest advancements related to all aspects of bone marrow. The manuscripts published in International Journal of Bone Marrow Research seeks to provide valuable information in bone marrow research, related diseases, transplant procedure and all aspects of follow-up care.. ...
Bone marrow stromal cells (BMSCs) constitute a cell population routinely used as a representation of mesenchymal stem cells in vitro. They reside within the bone marrow cavity alongside hematopoietic stem cells (HSCs), which can give rise to red blood cells, immune progenitors, and osteoclasts. Thus, extractions of cell populations from the bone marrow results in a very heterogeneous mix of various cell populations, which can present challenges in experimental design and confound data interpretation. Several isolation and culture techniques have been developed in laboratories in order to obtain more or less homogeneous populations of BMSCs and HSCs invitro. Here, we present two methods for isolation of BMSCs and HSCs from mouse long bones: one method that yields a mixed population of BMSCs and HSCs and one method that attempts to separate the two cell populations based on adherence. Both methods provide cells suitable for osteogenic and adipogenic differentiation experiments as well as functional assays
BACKGROUND: The expression of the two types of ferritin subunits, the H-subunit and L-subunit, has been shown to be differentially regulated by cytokines. The primary aim of the present study was to quantitatively measure the expression of the H-subunit and L-subunit of ferritin in bone marrow macrophages and cells of the erythron in patients with chronic T-helper cell type-1 immune stimulation. METHODS: The expression of the H-subunit and L-subunit of ferritin in bone marrow macrophages and cells of the erythron was quantitatively evaluated by post-embedding immunolocalisation with immunogold transmission electron microscopy. RESULTS: The present study showed up-regulation of the H-subunit of ferritin in the bone marrow macrophage in patients with pronounced cellular immune activation (94.7 ± 37.3 counts/μm2; n = 31 vs 72.4 ± 34.0 counts/μm2; n = 13, p-value = 0.037). CONCLUSION: This supports a possible role for H-subunit rich ferritins in the hypoferraemia of chronic disease ...
Rafał B. Lewandowski, Małgorzata Stępińska, Andrzej Gietka, Monika Dobrzyńska, Mariusz P. Łapiński & Elżbieta A. Trafny (2021) The red-light emitting diode irradiation increases proliferation of human bone marrow mesenchymal stem cells preserving their immunophenotype, International Journal of Radiation Biology, 97:4, 553-563, DOI: 10.1080/09553002.2021.1876947 Abstract Purpose For effective clinical application of human bone marrow mesenchymal stem cells (hBM-MSCs), the enhancement of their proliferation ...
TY - JOUR. T1 - Intravenous Bone Marrow Mononuclear Cells for Acute Ischemic Stroke. T2 - Safety, Feasibility, and Effect Size from a Phase I Clinical Trial. AU - Vahidy, Farhaan S.. AU - Haque, Muhammad E.. AU - Rahbar, Mohammad H.. AU - Zhu, Hongjian. AU - Rowan, Paul. AU - Aisiku, Imoigele P.. AU - Lee, Dean A.. AU - Juneja, Harinder S.. AU - Alderman, Susan. AU - Barreto, Andrew D.. AU - Suarez, Jose I.. AU - Bambhroliya, Arvind. AU - Hasan, Khader M.. AU - Kassam, Mallikarjuna Rao. AU - Aronowski, Jaroslaw. AU - Gee, Adrian. AU - Cox, Charles S.. AU - Grotta, James C.. AU - Savitz, Sean I.. PY - 2019/11/1. Y1 - 2019/11/1. N2 - Cellular therapy is a promising investigational modality to enhance poststroke recovery. We conducted a single-arm, phase I clinical trial to determine the safety and feasibility of intravenous (IV) administration of autologous bone marrow mononuclear cells (MNCs) after acute ischemic stroke (AIS). Patients with moderate severity of AIS underwent bone marrow harvest ...
B Zakhireh, RK Root; Development of Oxidase activity by human bone marrow granulocytes, Blood, Volume 54, Issue 2, 1 August 1979, Pages 429-439, https://doi.org
P220 To determine the degree and extent of changes in cellular metabolic demand after stroke and bone marrow cell transplantation, a histochemistry assay of cytochrome oxidase (COx) which correlates with neuronal activity was employed,. Adult Wistar rats (n=9) were subjected to transient (2 h) middle cerebral artery occlusion (MCAo). At 1 d after ischemia, bone marrow stromal cells (MSCs, 4x105 in 10 :l) were transplanted intracerebrally into the ischemic boundary zone in the striatum and the cortex. The ischemia rats with (n=4) or without (n=5) MSC transplantation were sacrificed at 14 d after MCAo. Bone marrow cells were harvested from normal donor adult rats and cultured in Iscove s Modified Dulbecco s medium supplemented with 10% fetal bovine serum. MSCs were isolated by their adherence to the plastic dishes from the whole bone marrow cells at 72 h of incubation. Subsequently, the MSCs were cultured for 2 weeks and bromodeoxyuridine (BrdU, as a tracer to identify cells derived from bone ...
Compare average cost of Bone Marrow Stem Cell Therapies in Mexico City, Mexico. Price of Bone Marrow Stem Cell Therapies starts from $103.
This year marks the 60th anniversary of one of the seminal publications that triggered the introduction of haematopoietic stem cell transplantation (HSCT) in medical practices worldwide. This landmark paper by Thomas et al. entitled, Intravenous infusion of bone marrow in patients receiving radiation and chemotherapy was published in the New England Journal on the 12th September 1957. The same year, this group also published other landmark papers in Blood. From the mid-1950s, Thomas developed methods for providing people with new bone marrow cells through transplants. Using radiation, chemotherapy, and nowadays immunosuppressive drugs, the bodys own bone marrow cells are killed and the immune systems rejection mechanism is subdued. Bone marrow cells from a donor are then provided through a blood transfusion. In 1958, a year after Thomas paper, Georges Mathé performed the first ever successful allogeneic bone marrow transplant on unrelated human beings. Since then, major developments in the ...
Bioencapsulation of cells is one of the many areas of artificial cells being extensively investigated by centers around the world. This includes the bioencapsulation of hepatocytes. A number of methods have been developed to maintain the specific function and phenotype of the bioencapsulated hepatocytes for in vitro and in vivo applications. These include supplementation of factors in the culture medium; use of appropriate substrates and the co-cultivation of hepatocytes with other type of cells, the so called feeder cells. These feeder cells can be of liver origin or non-liver origin. We have recently studied the role of bone marrow cells in the maintenance of hepatocytes viability and phenotype by using the coculture of hepatocytes with bone marrow cells (nucleated cells including stem cells), and the coencapsulation of hepatocytes with bone marrow stem cells. This way, the hepatocytes viability and specific function can be maintained significantly longer. In vivo studies of both syngeneic and
Purpose: : To study the role of in-vitro generated Bone Marrow derived Dendritic Cells (BMDC) after glucocorticoid treatment on corneal allograft survival in the rat. Methods: : BMDC were propagated from either Lewis (LEW) or Dark Agouti (DA) rat bone marrow precursors cells (1.5x106 cells/ml) in complete medium supplemented with rat GMCSF (5ng/µl) and IL-4 (5ng/µl). For glucocorticoid treatment of BMDCs, dexamethasone (Dexa) (10-6M) was added on d5 and d7 of a 10 day culture. BMDC and Dexa BMDC phenotype was characterised and analysed for expression of cell surface markers CD11b/c, MHC II, CD80, CD86 and His36 by flow cytometry. BMDC and Dexa BMDC antigen presenting cell function was examined in both antigen specific (Ovalbumin) and allo-antigen specific lymphocyte assays. Responder cells were analysed by FACS for proliferation and expression of lymphocyte activation markers CD25 or OX40. Moreover recall experiments were performed to study the mechanisms of immunomodulation in vivo. A fully ...
The objective of this study was to examine the osteoinductive capacity of different concentrations of BMP-2 on bone marrow stromal cells in vitro. Further, we intended to determine whether titanium provided with an increased surface roughness is more efficient in osteoblast differentiation than machined titanium. Therefore, 20,000 cells/ml were seeded and cultured on machined and grit-blasted titanium discs for 4, 8 and 16 days. Different concentrations of rhBMP-2 (0, 10, 100, 1000 ng/ml) were supplemented to the medium for 8 days of culturing. To evaluate cellular proliferation and differentiation, specimens were examined for DNA, alkaline phosphatase activity, and calcium content. Morphological appearance of the specimens at 8 and 16 days of incubation was evaluated using scanning electron microscopy. Two separate experimental runs were performed.Evaluation of the DNA and alkaline phosphatase data revealed that a significant difference existed for these data between both experimental runs. ...
TY - JOUR. T1 - Characterization of NKR+ T-cell subsets in human bone marrow. T2 - implications for immunosurveillance of neoplasia. AU - Dean, J. AU - McCarthy, D. AU - Doherty, D G. AU - OFarrelly, C. AU - Golden-Mason, L. AU - Lawler, Mark. PY - 2005/1. Y1 - 2005/1. N2 - In addition to hematopoietic progenitors, human bone marrow contains mature T/NK lymphocytes. Valpha24Vbeta11 NKT-cells, a subset of NK receptor+ (NKR+) T-cells in humans, are rare in bone marrow, suggesting the presence of other NKR+ T-cells which may contribute to tumor surveillance. NKR+/- T-cells were examined in blood (PB), and bone marrow from donors (DM) and patients with active hematopoietic malignancy (PM), or in remission (PR). T-cells in PR & PM were enriched for CD56+ and CD57+ subsets, compared to DM. All marrow NKR+/- T-cell subsets were more activated than PB. PM and, surprisingly, PR marrow contained more activated cells than DM. CD8+ cells were significantly increased in all patient marrows and there was ...
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... or bone marrow (less than 10 percent of nucleated bone marrow cells are tumors). ... He also noted the circular clumps of cells in bone marrow samples which are now termed "Homer Wright rosettes". Of note, "Homer ... Stage 4: Dissemination of tumor to distant lymph nodes, bone marrow, bone, liver, or other organs except as defined by Stage 4S ... bone marrow / hematopoietic stem cell transplantation,[46] biological-based therapy with 13-cis-retinoic acid (isotretinoin or ...
June 2008). "Stem cell transplantation for primary immunodeficiencies". Bone Marrow Transplant. 41 Suppl 2: S83-6. doi:10.1038/ ... cell responses to mitogens and allogeneic cells, cytokine production by cells Tests for B cell function: antibodies to routine ... natural killer cells and monocytes (CD15+), as well as activation markers (HLA-DR, CD25, CD80 (B cells). Tests for T cell ... Bone marrow transplant may be possible for Severe Combined Immune Deficiency and other severe immunodeficiences. Virus-specific ...
Hathaway WE, Newby LA, Githens JH (1964). "THE ACRIDINE ORANGE VIABILITY TEST APPLIED TO BONE MARROW CELLS. I. CORRELATION WITH ... the living cells exclude the stain i.e. stain negatively and only the dead cells stain positively and thus viability can be ... apoptotic and normal cells. Trypan Blue, a living-cell exclusion dye Erythrosine, which is Red No. 3 in food coloring, can be ... A vital stain in a casual usage may mean a stain that can be applied on living cells without killing them. Vital stains have ...
"Bone Marrow (Hematopoietic) Stem Cells , stemcells.nih.gov". stemcells.nih.gov. Retrieved 2017-07-13. Sullivan, Kathleen E.; ... which are stem cells that bring about other cells) has proven useful in some instances. Additionally the following treatments ... V(D)J recombination is a genetic recombination that happens in early stages of B and T cell maturation. The diagnosis of ... Recurrent infections Microcephaly Growth retardation Bone-malformation Dysmorphic feature Urogenital malformations In terms of ...
Hess DC, Hill WD, Carroll JE, Borlongan CV (Apr 2004). "Do bone marrow cells generate neurons?". Archives of Neurology. 61 (4 ... Kemp K, Wilkins A, Scolding N (Nov 2014). "Cell fusion in the brain: two cells forward, one cell back". Acta Neuropathologica. ... "Fusion of bone-marrow-derived cells with Purkinje neurons, cardiomyocytes and hepatocytes". Nature. 425 (6961): 968-73. doi: ... "Stable reprogrammed heterokaryons form spontaneously in Purkinje neurons after bone marrow transplant". Nature Cell Biology. 5 ...
"Investigative Engineered Bone Marrow Cell Therapy". Fred Hutchinson Cancer Research Center. 2011-05-23. Dall'oglio S, D'Amico A ... The most common side effect is bone marrow suppression. The most common non-hematological adverse effects associated with ... This methylation damages the DNA and triggers the death of tumor cells. However, some tumor cells are able to repair this type ... Because tumor cells that express the MGMT gene are more resistant to the effects of temozolomide, researchers investigated ...
Adult stem cells like bone marrow stem cells have also shown a potential to differentiate into cardiac competent cells when ... "The histone methyltransferase inhibitor BIX01294 enhances the cardiac potential of bone marrow cells.". Stem Cells Dev. 22: 654 ... Costa S, Shaw P (March 2007). "'Open minded' cells: how cells can change fate" (PDF). Trends Cell Biol. 17 (3): 101-6. PMID ... In mammals, most cells terminally differentiate, with only stem cells retaining the ability to differentiate into several cell ...
Wickramasinghe SN, Olsen I, Saunders JE (1975). "Thymidine kinase activity in human bone marrow cells". Scandinavian Journal of ... is cell cycle-independent. TK1 is synthesized by the cell during the S phase of cell division. After cell division is completed ... in peripheral lymphocytes during monocytosis and in bone marrow during pernicious anemia. As TK1 is present in cells during ... Enzymes of thymidine and thymidylate metabolism in normal and pathological blood and bone marrow cells]". Blut (in German). 25 ...
These are multipotent cells that reside in the bone marrow niche and have the ability to give rise to all heamatopoetic cells, ... expression in bone marrow and fractionated marrow cell populations by interleukin 3 (IL-3): IL-3-mediated positive feedback ... These are secreted by other haematopoietic cells in the bone marrow or at the site of inflammation as well as epithelial and ... Bendall, Linda J.; Bradstock, Kenneth F. (2014-08-01). "G-CSF: From granulopoietic stimulant to bone marrow stem cell ...
"Bone marrow cell treatment for chronic multiple sclerosis". MS Trust website. Retrieved 29 November 2012. "Pilates based core ... Research currently being funded includes: University of Bristol, Bone marrow cell treatment for chronic multiple sclerosis ...
... in the bone marrow. Various chemokines and receptors are involved in the homing of hematopoietic stem cells. Bone Marrow Lymph ... Yusuf, Rushdia Z.; Scadden, David T. (17 March 2009). "Homing of Hematopoietic Cells to the Bone Marrow". Journal of Visualized ... Homing is the phenomenon whereby cells migrate to the organ of their origin. By homing, transplanted hematopoietic cells are ... "Chemokine receptors that mediate B cell homing to secondary lymphoid tissues are highly expressed in B cell chronic lymphocytic ...
GvHD is commonly associated with bone marrow transplants and stem cell transplants. White blood cells of the donor's immune ... International Bone Marrow Transplant Registry". Bone Marrow Transplantation. 24 (3): 283-7. doi:10.1038/sj.bmt.1701899. PMID ... "Stem Cell or Bone Marrow Transplant Side Effects". www.cancer.org. Retrieved 2020-09-01. Goker H, Haznedaroglu IC, Chao NJ ( ... After bone marrow transplantation, T cells present in the graft, either as contaminants or intentionally introduced into the ...
Morrison, S. J; Scadden, D. T (2014). "The bone marrow niche for haematopoietic stem cells". Nature. 505 (7483): 327-334. doi: ... Often, cell debris and foreign particles, which are impermeable to the BBB will get through the endothelial cells, only to be ... The production of cAMP aids in the modulation of auto-reactive T cells by regulatory T cells. . The perivascular space is ... This holds true for many T and B cells, as well as monocytes, giving this small fluid filled space an important immunological ...
... s are produced by the bone marrow from precursors called monoblasts, bipotent cells that differentiated from ... "Bone Marrow Endothelial Cells Regulate Myelopoiesis in Diabetes". Circulation. 142 (3): 244-258. doi:10.1161/CIRCULATIONAHA. ... Vacuolization may be present in a cell that has recently phagocytized foreign matter. Many factors produced by other cells can ... Monocytes are also capable of killing infected host cells via antibody-dependent cell-mediated cytotoxicity. ...
Anti-Kell suppresses the bone marrow,[8] by inhibiting the erythroid progenitor cells.[9][10] ... It works by binding any fetal red blood cells with the D antigen before the mother is able to produce an immune response and ... Among these antibodies are some which attack antigens on the red blood cells in the fetal circulation, breaking down and ... Reticulocyte count - Reticulocytes are elevated when the infant is producing more red blood cells in response to anemia.[25] A ...
... and c-kit-dependent bone marrow". Cell. 135 (3): 437-48. doi:10.1016/j.cell.2008.08.041. PMC 2788814. PMID 18984156. Lay ... bone marrow suppression, liver problems, and heart failure.[2] Use during pregnancy may result in harm to the baby.[2] Imatinib ... Some tumor cells, however, have a dependence on bcr-abl.[28] Inhibition of the bcr-abl tyrosine kinase also stimulates its ... This can slow growth or result in programmed cell death of certain types of cancer cells.[2] ...
Cellular factors include reduced number and function of bone-marrow derived endothelial progenitor cells.[17] and reduced ... Sinusoid blood vessels are primarily located in the bone marrow, lymph nodes,[citation needed] and adrenal glands. Some ... where greater movement of cells and materials is necessary.[citation needed] A capillary wall is only 1 cell thick and is ... and therefore utilize gaps present in cell junctions to permit transfer between endothelial cells, and hence across the ...
... which are cancers of the blood-forming cells of the bone marrow. In adults, blood cells are formed in the bone marrow, by a ... Bone marrow aspirates will display hypercellularity with increased counts of granulocytic and monocytic cells. Bone marrow core ... in the peripheral blood and bone marrow, as well as abnormal looking cells (dysplasia) in at least one type of blood cell. CMML ... 2.5 x 109/L, >0% immature myeloid cells, >10% bone marrow blasts causes a reduced overall survival. This data allows cases of ...
"Laminin isoform-specific promotion of adhesion and migration of human bone marrow progenitor cells". Blood. 101 (3): 877-85. ... regulation of cell adhesion. • cell adhesion. • extracellular matrix organization. • regulation of cell migration. • regulation ... "The alpha4 laminin subunit regulates endothelial cell survival". Experimental Cell Research. 294 (1): 281-9. doi:10.1016/j. ... Mrowiec T, Melchar C, Górski A (1998). "HIV-protein-mediated alterations in T cell interactions with the extracellular matrix ...
Cellular factors include reduced number and function of bone-marrow derived endothelial progenitor cells.[20] and reduced ... Sinusoids are irregular spaces filled with blood and are mainly found in the liver, bone marrow, spleen, and brain ... bone marrow, anterior pituitary gland, and brain circumventricular organs. Capillaries and sinusoids are short vessels that ... and therefore utilize gaps present in cell junctions to permit transfer between endothelial cells, and hence across the ...
BAFF is secreted by a variety of cells: monocytes and macrophages; bone marrow stromal cells; astrocytes in certain ... B cells develop in the bone marrow and continue to mature peripherally in secondary lymphoid organs and in the gut. When ... Belimumab binds to BAFF and prevents it from binding to B cells. Without BAFF, B cells commit suicide and no longer contribute ... Researchers theorize that SLE is caused when autoimmune B cells proliferate and survival factors protect them from cell suicide ...
"Vasculogenic mimicry of acute leukemic bone marrow stromal cells". Leukemia. 23 (6): 1039-1048. doi:10.1038/leu.2009.10. PMID ... Vaudry, D.; Stork, PJ; Lazarovici, P; Eiden, LE (31 May 2002). "Signaling Pathways for PC12 Cell Differentiation: Making the ... in PC12 Cells". Journal of Molecular Neuroscience. 54 (3): 574-585. doi:10.1007/s12031-014-0388-2. PMID 25078264. S2CID 1620005 ... Endothelial Cell Tube Formation Assay". Neurotrophic Factors. Methods in Molecular Biology. 1727. pp. 239-250. doi:10.1007/978- ...
Plasma cells less than 10% on bone marrow examination; No evidence of bone lesions, anemia, hypercalcemia, or chronic kidney ... There is a predominance of clonal plasma cells in the bone marrow with an abnormal immunophenotype (CD38+ CD56+ CD19−) mixed in ... MGUS is a common, age-related medical condition characterized by an accumulation of bone marrow plasma cells derived from a ... white blood cells that secrete antibodies) in the bone marrow is lower, and it rarely has symptoms or major problems. However, ...
NK Cells), Haemopoietic Bone Marrow Stem Cells CD34/ 38, 45 Scatter gating CD45, ICH on FFPE tissue with Mabs T& B and NK ... Stem cell from FACS II for Bone Marrow Transplantation…..Immunology 1978 34 625T-lymphocyte dependency of B-lymphocyte ... IL-2 T cell growth factors Cytokines and Histamine CD8 Suppressor T Cells. LIF , FACS IV cytometry analysis , IL-2 The T-cell ... Immunotherapy basics CTLA-4, Mesenchymal stem cells and Dendritic cells Pathways Multi-color FITC/ PE and PerCP 2-3 color ...
Bone marrow is the primary site of new blood cell production or hematopoiesis. It is composed of hematopoietic cells, marrow ... All white blood cells are produced and derived from multipotent cells in the bone marrow known as hematopoietic stem cells. ... Beta cell - Beta cellscells) are a type of cell found in pancreatic islets that synthesize and secrete insulin. Beta cells ... Zygomatic bone - In the human skull, the zygomatic bone (cheekbone or malar bone) is a paired irregular bone which articulates ...
Debernardi S, Dixon-McIver A (2010). "MicroRNA detection in bone marrow cells by LNA-FISH". MicroRNAs and the Immune System. ... Wong TS, Liu XB, Wong BY, Ng RW, Yuen AP, Wei WI (May 2008). "Mature miR-184 as Potential Oncogenic microRNA of Squamous Cell ... Choong ML, Yang HH, McNiece I (April 2007). "MicroRNA expression profiling during human cord blood-derived CD34 cell ... "Differential microRNA expression in peripheral blood mononuclear cells from Graves' disease patients". The Journal of Clinical ...
Boes KM, Durham AC (February 2017). "Bone Marrow, Blood Cells, and the Lymphoid/Lymphatic System.". Pathologic Basis of ... Thrombopoiesis is the formation of platelets in the Bone marrow. Thrombopoietin is the main regulator of thrombopoiesis. ... and supporting these cells so they mature to become platelet-producing cells. The process of Thrombopoiesis is caused by the ... Megakaryocytes are precursor cells that are highly specialized. Megakaryocytes give rise to 1,000 to 3,000 platelets. ...
A CXCR4 agonist pepducin mobilizes bone marrow hematopoietic cells. A PAR1 pepducin, PZ-128, has successfully completed phase I ... "Discovery of a CXCR4 agonist pepducin that mobilizes bone marrow hematopoietic cells". 2010. Archived from the original on 2011 ... Pepducins are cell-penetrating peptides that act as intracellular modulators of signal transference from receptors to G ... This structure allows pepducin lipopeptides to anchor in the cell membrane lipid bilayer and target the GPCR/G protein ...
... a surface molecule of bone marrow stromal cell lines that facilitates pre-B-cell growth". Proc Natl Acad Sci U S A. 91 (12): ... Bst1 (Bone marrow stromal cell antigen 1, ADP-ribosyl cyclase 2, CD157) is an enzyme that in humans is encoded by the BST1 gene ... "Entrez Gene: BST1 bone marrow stromal cell antigen 1". Quarona V, Zaccarello G, Chillemi A (2013). "CD38 and CD157: a long ... 1996). "Elevated levels of the soluble form of bone marrow stromal cell antigen 1 in the sera of patients with severe ...
Plasma cells originate in the bone marrow; B cells differentiate into plasma cells that produce antibody molecules closely ... After leaving the bone marrow, the B cell acts as an antigen presenting cell (APC) and internalizes offending antigens, which ... Plasma cells, also called plasma B cells, plasmocytes, plasmacytes, or effector B cells, are white blood cells that secrete ... In humans, CD27 is a good marker for plasma cells, naive B cells are CD27-, memory B-cells are CD27+ and plasma cells are ...
... bone marrow transplantation, and HIV-1 infection. Annual Review of Immunology. 2000, 18: 529-560. ISSN 0732-0582. PMID 10837068 ... T Cells to protect tumour cells. Nature Communications. March 2018, 9 (1): 948. PMC 5838096. PMID 29507342. doi:10.1038/s41467- ... 细胞毒性T细胞(CTLs, killer T cells)负责杀伤被病毒感染的细胞和癌细胞,在对器官移植的免疫排斥中也有参与。其特点在于细胞表面的CD8蛋白质。它通过识别所有有核细胞
2009). "Meningioma 1 gene is differentially expressed in CD34 positive cells from bone marrow of patients with myelodysplastic ... "Blood Cells Mol. Dis. 39 (3): 336-9. doi:10.1016/j.bcmd.2007.06.009. PMC 2387274. PMID 17698380.. ... Cell. Proteomics. 7 (3): 499-508. doi:10.1074/mcp.M700325-MCP200. PMID 18029348.. ... 2D3-regulated transcription factor MN1 stimulates vitamin D receptor-mediated transcription and inhibits osteoblastic cell ...
... marrow adipocytes and beta-pancreatic islets cells. Cyfeiriadau[golygu , golygu cod y dudalen]. *↑ Vunjak-Novakovic, G.; Tandon ... Bone-marrow Arall. Ffeiliau perthnasol ar Gomin Wicimedia. Mer esgyrn yw'r meinwe hyblyg tu mewn asgwrn. Gyda bodau dynol, mae ... macroffabau, sy'n cyfrannu'n sylweddol at gynhyrchiad Cell goch y gwaed, gan eu bod yn mynd a Haearn ar gyfer cynhyrchiad ... Mae'n hysbys fod MSCau yn addasu, yn vitro neu'n vivo, i osteoblastau, chondroctyeau (cell cartilag), have been shown to ...
"Increased expression of preprotachykinin-I and neurokinin receptors in human breast cancer cells: implications for bone marrow ... Substance P has been known to stimulate cell growth in normal and cancer cell line cultures,[37] and it was shown that ... on cells (including cancer cells) bestowing upon them mobility.[40] and metastasis.[41] It has been suggested that cancer ... stem cells, white blood cells) in many tissues and organs. SP amplifies or excites most cellular processes.[15][16] ...
Thomas' work showed that bone marrow cells infused intravenously could repopulate the bone marrow and produce new blood cells. ... "Bone Marrow Transplant" redirects here. For the journal abbreviated Bone Marrow Transplant, see Bone Marrow Transplantation ( ... Stem cell transplantation was pioneered using bone-marrow-derived stem cells by a team at the Fred Hutchinson Cancer Research ... Unlike other organs, bone marrow cells can be frozen (cryopreserved) for prolonged periods without damaging too many cells. ...
"Improved liver function in patients with liver cirrhosis after autologous bone marrow cell infusion therapy". Stem Cells 24 (10 ... "Index of CD34+ Cells and Mononuclear Cells in the Bone Marrow of Spinal Cord Injury Patients of Different Age Groups: A ... stem cells in adult bone marrow and other tissues". Leukemia 21 (5): 860-7. PMID 17344915. ... "P-Selectin coated microtube for enrichment of CD34+ hematopoietic stem and progenitor cells from human bone marrow". Clin Chem ...
Osteochondroprogenitor cells are progenitor cells that arise from mesenchymal stem cells (MSC) in the bone marrow. They have ... before any genetic or morphological criteria were put in place for bone marrow or connective tissues. Osteoprogenitor cells can ... giving rise to either bone or cartilage respectively. Osteochondroprogenitor cells are important for bone formation and ... Osteoblasts are cells that group together to form units, called osteons, to produce bone. Runx2 (which may also be known as ...
The organization hosts events throughout the year to support the hematology/oncology/bone marrow transplant unit[71] at ... engineering students and engineers from the Ford Motor Company and will seek to break the land speed record for hydrogen cell ...
Talk:Boettcher cell. *Talk:Bone marrow. *Talk:Bone tissue. *Talk:Bony labyrinth ...
Form a scaffolding for other cells. Type III collagen. liver, bone marrow, and lymphatic organs ... cartilage and bone.[15]:158 Cells of the immune system, such as macrophages, mast cells, plasma cells and eosinophils are found ... Bind bones and other tissues to each other. Alpha polypeptide chains. tendon, ligament, skin, cornea, cartilage, bone, blood ... The cells of connective tissue include fibroblasts, adipocytes, macrophages, mast cells and leucocytes. ...
... clots of fat from the bone marrow can escape from the broken bone and travel to the lungs) ... However, it can also be caused by clumped cancer cells, fat, or bone. Rarely, while giving birth, a woman can get a clot of ... Recent fracture of one of the long bones in the leg (because having a broken leg makes it harder to move around; also, ...
Jude is the first known hospital in the world to cure sickle cell disease through bone marrow transplantation. Today, bone ... marrow transplantation still offers the only cure for sickle cell disease. Members of Kappa Alpha Psi reach out to churches in ... Members of Kappa Alpha Psi have committed to raise $500,000 in support of the hospital's sickle cell program. St. Jude has one ... Since that time, members across the country have joined in the fight against pediatric cancer, sickle cell disease, and other ...
These M cells then alert the underlying B cells and T cells in the tonsil that a pathogen is present and an immune response is ... The tonsils have on their surface specialized antigen capture cells called M cells that allow for the uptake of antigens ... Bone marrow. *Hematopoietic stem cell. Thymus. *Hassall's corpuscles. Secondary organs. Spleen. *Structure *Hilum ... "Tonsils Make T-Cells, Too, Ohio State Study Shows". Ohio State University. Ohio State University, Comprehensive Cancer Center. ...
"Bone marrow suppression". Chemotherapy Principles: An In-depth Discussion. American Cancer Society. Archived from the original ... In oncology, the term nadir is used to represent the lowest level of a blood cell count while a patient is undergoing ...
Discontinuous capillaries as found in sinusoidal tissues of bone marrow, liver and spleen have little or no filter function.[1] ... Where the endothelial glycocalyx overlies an inter endothelial cell cleft, the plasma ultrafiltrate may pass to the ...
... which is the organ essential for T-cell maturation following the migration of precursor cells from the bone marrow. This age- ... "Enhanced differentiation of splenic plasma cells but diminished long-lived high-affinity bone marrow plasma cells in aged mice ... The cytotoxicity of Natural Killer (NK) cells and the antigen-presenting function of dendritic cells is known to diminish with ... Mocchegiani, E; M. Malavolta (2004). "NK and NKT cell functions in immunosenescence". Aging Cell. 3 (4): 177-184. doi:10.1111/j ...
Then he sutured them together and attached them to the stump of bone to keep them from retracting.[16] This is the first known ... Gift of Life Marrow Registry. *Human Tissue Authority. *LifeSharers. *National Marrow Donor Program ... Surgeons usually connect the bones first, followed by tendons, arteries, nerves, veins, and skin. ...
... using tissue engineering with a scaffold derived from a rat meniscus and mesenchymal stromal cells derived from rat bone marrow ... Then these cells are injected into the patient. These cells are held in place by a small piece of soft tissue from the tibia, ... 10,000 cells are harvested and grown in vitro for approximately six weeks until the population reaches 10-12 million cells. ... is a biological treatment option for articular cartilage damage bone marrow stimulating technique in combination with a ...
... bone marrow - bone marrow suppression - booster - branched DNA assay - breakthrough infection - Broadway Cares/Equity Fights ... T suppressor cells - T4 cell - T4 cells (T-helper cells) - T8 cells - Tanner staging - TAT - TB - template - TeachAIDS - ... B-cell lymphoma - B cells - B lymphocytes (B cells) - bactericidal - bacteriostatic - bacterium - baculovirus - baseline - ... cells - CDC National Prevention Information Network (CDC-NPIN) - cell lines - cell-mediated immunity (CMI) - cellular immunity ...
All white blood cells are produced and derived from multipotent cells in the bone marrow known as hematopoietic stem cells. ... T cells: *CD4+ helper T cells: T cells displaying co-receptor CD4 are known as CD4+ T cells. These cells have T-cell receptors ... B cells: releases antibodies and assists activation of T cells. *T cells: *CD4+ Th (T helper) cells: activate and regulate T ... Natural killer cells: virus-infected and tumor cells.. Deeply staining, eccentric. NK-cells and cytotoxic (CD8+) T-cells. Years ...
"Journal of Stem Cells and Regenerative Medicine.. *↑ "Index of CD34+ Cells and Mononuclear Cells in the Bone Marrow of Spinal ... Updated: 16 December 2013 Why Perform a Stem Cell Transplant?. *↑ Bone Marrow Transplantation and Peripheral Blood Stem Cell ... "Improved liver function in patients with liver cirrhosis after autologous bone marrow cell infusion therapy". Stem Cells 24 (10 ... Bone Marrow Transplant». ucsfchildrenshospital.org.. *↑ Ed Kane. «Stem-cell therapy shows promise for horse soft-tissue injury ...
This agent also causes respiratory tract lesions, bone marrow depression, and eye damage, the epithelial tissues of these ... 57:51-9. Tang PS, Mura M, Seth R, Liu M. (2008) Acute lung injury and cell death: how many ways can cells die? Am J Physiol 294 ... There are two types of alveolar epithelial cells - Type 1 pneumocytes represent 90% of the cell surface area, and are easily ... Furthermore, when phosgene hydrolyzes it forms hydrochloric acid, which can damage the cell surface and cause cell death in the ...
For a long time, the most efficient approach had been to use bone marrow graft, or hematopoietic stem cell transplantation. ... Because of all these reasons, bone marrow grafts or hematopoietic stem cell transplantation have seen a decrease in their ... The matrix surrounds the cells of the body in an organized meshwork and functions as the glue that holds the cells of the body ... Nearly every cell in the human body has 46 chromosomes, with 23 derived from each parent. The IDS gene is located on the X ...
"Effect of homologous bone marrow injections in x-irradiated rabbits". British Journal of Experimental Pathology. 38 (4): 401- ... Cell biologyEdit. Main article: Biomarker (cell). In cell biology, a biomarker is a molecule that allows the detection and ... Basu, P. K.; Miller, I.; Ormsby, H. L. (1960-03-01). "Sex chromatin as a biologic cell marker in the study of the fate of ... Neuronal cell body injury markers include Neuron-specific enolase (NSE), Ubiquitin C-terminal hydrolase-L1 (UCH-L1), Astroglial ...
Increased migration of cord blood-derived CD34+ cells, as compared to bone marrow and mobilized peripheral blood CD34+ cells ... KK Ballen, F Verter and J Kurtzberg Umbilical cord blood donation: public or private? Bone Marrow Transplantation (2015), 1-8 ... Human umbilical cord blood as a potential source of transplantable hematopoietic stem/progenitor cells. Proceedings of the ...
They are usually the first cells to arrive at the site of an infection.[5] The bone marrow of a normal healthy adult produces ... Instead, NK cells destroy compromised host cells, such as tumor cells or virus-infected cells. It recognises such cells by a ... Mast cells[change , change source]. Main article: Mast cell. Mast cells are a type of innate immune cell in connective tissue ... Natural killer cells[change , change source]. Main article: Natural killer cell. Natural killer cells, or NK cells, are a part ...
Anatomical terms of bone. নিতম্বাস্থি (ইনোমিনেট অস্থি, পেলভিক অস্থি[১]) একটি বড় সমতল অস্থি,যা দেহের কেন্দ্রে থাকে এবং উপর ও ... আলোকগ্রাহক কোষ (Photoreceptor cell). *দণ্ড কোষ (Rod cell). *শঙ্কু কোষ (Cone cell) ... অস্থি মজ্জা (Bone marrow). *থাইমাস গ্রন্থি (Thymus). *প্লীহা (Spleen). *তালুমূলগ্রন্থি (Tonsil). *লসিকাকোষ (Lymphocyte) ... Merriam Webster, http://www.merriam-webster.com/medical/hip+bone *↑ ক খ গ Bojsen-Møller, Finn; Simonsen, Erik B.; Tranum-Jensen ...
Campbell was the first to discover that cells of bone marrow origin contribute to intimal (the innermost coat of blood vessels ... Her most recent work involves the development of autologous vascular grafts from cells of bone marrow, known as the myeloid, ... she was the first to discover that smooth muscle cells can exist in a spectrum of phenotypes that control the cell's biology ... Professor Julie Campbell, Cell Biologist and Professorial Fellow at the Australian Institute for Bioengineering and ...
A study now combines single-cell and spatial transcriptomics with imaging to infer the cellular composition and spatial ... Different types of stromal cells in the bone marrow associate to form niches that support differentiating blood cells and ... its location in the bone marrow, differential and regional bone marrow cytokine enrichment and candidate interacting cells ... Different types of stromal cells in the bone marrow associate to form niches that support differentiating blood cells and ...
Since it is a simple procedure to collect stem cells from bone marrow, we hope that our research paves the way forward into ... Researchers based in Louisiana showed that therapy with human multipotent stromal cells (hMSC) isolated from bone marrow was ... investigates the therapeutic use of human stem cells from bone marrow against acute lung injury and identifies TNF-Ã ±-induced ... "Stem cell therapy shows great promise in the treatment of acute and life threatening conditions, such as acute lung injury and ...
Injecting the hearts of angina sufferers with cells extracted from their own bone marrow can reverse the condition and relieve ... The researchers are now investigating which types of bone-marrow cell best help repair the heart. One theory is that a type of ... Healing the Heart with Bone-Marrow Cells. A new treatment may help angina sufferers who are resistant to surgery and medication ... The researchers then took bone marrow from participants hips and extracted the mass of mononuclear cells-an ill-defined mix of ...
Stem cells from adult bone marrow normally generate bone, muscle, cartilage and fat cellsa limited set compared with embryonic ... Prior research with cultured tissue had shown that a mix of chemicals could change bone marrow stem cells from mice to those ... New findings suggest that a biochemical cocktail can coax adult bone marrow stem cells to become neurons, according to a report ... Scientists Find Wild Card Stem Cells in Bone Marrow. May 4, 2001 - Sergio Pistoi ...
Dendritic cell isolation from mouse body tissues can be difficult and the number of cells isolated small. This protocol ... the study of these cells is critical to gain a more complete understanding of their function. ... describes the growth of large number of dendritic cells from the culture of mouse ... While much is understood about dendritic cells and their role in the immune system, ...
These stem cells have been used to cure diseases since the 1960s. ... Stem cells from bone marrow form a number of cell types of the immune and circulatory system. ... Stem cells from bone marrow form a number of cell types of the immune and circulatory system. These stem cells have been used ...
Functional neural stem cells derived from adult bone marrow.. Bonilla S1, Silva A, Valdés L, Geijo E, García-Verdugo JM, ... Pluripotent hematopoietic cells from adult bone marrow may give rise not only to neurons, oligodendrocytes and astrocytes after ... demonstrating the ability of adult bone marrow progenitors to generate self-renewing, functional neural stem cells, validating ... Furthermore, the bone marrow-derived NSC differentiate in vivo into functional oligodendrocytes and neurons following ...
F1 hybrid mice transplanted with parental bone marrow from either parent A or... ... Bone Marrow Graft Rejection as a Function of TNK Cells. In: Sitkovsky M.V., Henkart P.A. (eds) Cytotoxic Cells: Recognition, ... The role of asialo GM 1+ cells in the resistance to transplants of bone marrow or other tissues. In: NK Cells and Other Natural ... A novel cell type responsible for marrow graft rejection in mice. T cells with NK phenotype cause acute rejection of marrow ...
The potential of bone marrow-derived cells to differentiate to glomerular mesangial cells. J. Am. Soc. Nephrol. 2001. 12:1401- ... Multipotent adult progenitor cells from bone marrow differentiate into functional hepatocyte-like cells. J. Clin. Invest. 2002 ... Multi-organ, multi-lineage engraftment by a single bone marrow-derived stem cell. Cell. 2001. 105:369-377. View this article ... After transplantation of bone marrow (BM) hematopoietic stem cells or nonhematopoietic mesenchymal stem cells, muscle (2), ...
... including bone marrow (BM), umbilical cord, skeletal muscle, and adipose tissue. MSCs secrete factors, including IL-6, M-CSF, ... endothelial cells, lung epithelial cells, hepatocytes, neurons, and pancreatic islets. MSCs have been identified as an adherent ... not only differentiate into types of cells of mesodermal lineage but also into endodermal and ectodermal lineages such as bone ... are prototypical adult stem cells with the capacity for self-renewal and differentiation with a broad tissue distribution. MSCs ...
We describe a nonenzymatic method for obtaining bone marrow cells by flushing chick long bone with a syringe filled with ... We describe a nonenzymatic method for obtaining bone marrow cells by flushing chick long bone with a syringe filled with ... Fuller, M.D., Gardner, R.M., Trueblood, M.S. et al. Primary cultivation of embryonic chick bone marrow cells. Journal of Tissue ... Primary cultivation of embryonic chick bone marrow cells. *Mike D. Fuller1. , ...
Recently we demonstrated that ATG at a clinically relevant concentration (10-50 mg/L) kills in vitro not only T cells but also ... In the present study, we investigated whether ATG kills not only leukemic blasts but also leukemic stem cells (LSCs). We used a ... We also measured ATG-mediated killing of healthy individuals hematopoietic stem cells (HSCs). Median 2% HSCs from blood and 15 ... flow cytometric assay of complement-mediated cytotoxicity (CDC). ATG-induced death of acute myeloid leukemia (AML) cells from ...
Bone marrow contains stem cells, which are immature cells that become blood cells. ... Bone marrow is the soft, fatty tissue inside your bones. ... Bone marrow contains stem cells, which are immature cells that ... There are two types of bone marrow donation:. *Autologous bone marrow transplant is when people donate their own bone marrow. " ... Bone marrow donation can be done either by collecting a donors bone marrow surgically, or by removing stem cells from a ...
Peripheral-blood stem cells versus bone marrow from unrelated donors.. Anasetti C1, Logan BR, Lee SJ, Waller EK, Weisdorf DJ, ... Peripheral-Blood Stem Cells versus Bone Marrow from Unrelated Donors. N Engl J Med. 2012 Oct 18;367(16):10.1056/NEJMoa1203517. ... Peripheral-Blood Stem Cells versus Bone Marrow from Unrelated Donors. N Engl J Med. 2012 Oct 18;367(16):10.1056/NEJMoa1203517. ... Peripheral-blood versus bone marrow stem cells. [N Engl J Med. 2013] ...
More than 50,000 stem cell transplants are performed each year worldwide. A research team led by Weill Cornell Medical College ... Finding a bone marrow donor match is challenging and the number of bone marrow cells from a single harvest procedure are often ... Bone marrow is the home of HSCs that produce all blood cells, including all types of immune cells. One treatment for patients ... Patients with some cancers may also need a bone marrow transplant when anticancer treatments damage the blood. Bone marrow ...
Breast cancer tumors can grow by recruiting other cells from bone marrow, lowering the chances of a patients survival, a new ... 26 (UPI) -- Breast cancer tumors can grow by recruiting other cells from bone marrow, lowering the chances of a patients ... New research suggests that breast cancers can increase their growth by recruiting stromal cells that form in bone marrow. Photo ... Breast cancer tumors may recruit bone marrow cells for growth. By Tauren Dyson ...
A mass of bone marrow cells may be grown in a culture supplemented with fibroblast growth factor-2 (FGF-2) and epidermal growth ... and in targeting delivery of cells transfected with a particular gene to diseased or damaged tissue. ... factor (EGF). Further methods of the present invention are directed to utilizing the neural progenitor cells cultured in this ... A method is described for generating a clinically significant volume of neural progenitor cells from whole bone marrow. ...
Patients with chronic heart failure given injections of their own bone marrow stem cells have better heart function and live ... Bone marrow stem cells used in the study were taken from the top of the patients pelvis and sorted in the lab before being ... The study included 391 patients, of whom 191 agreed to have the bone marrow stem cell treatment and 200 did not. After five ... STOCKHOLM (Reuters) - Patients with chronic heart failure given injections of their own bone marrow stem cells have better ...
J. Sanchez-Ramos, S. Song, F. Cardozo-Pelaez et al., "Adult bone marrow stromal cells differentiate into neural cells in vitro ... "The development of fibroblast colonies in monolayer cultures of guinea-pig bone marrow and spleen cells," Cell and Tissue ... multi-lineage engraftment by a single bone marrow-derived stem cell," Cell, vol. 105, no. 3, pp. 369-377, 2001. View at ... Therapy Effects of Bone Marrow Stromal Cells on Ischemic Stroke. Xinchun Ye, Jinxia Hu, and Guiyun Cui ...
... attempt to repair damaged heart of 16-year-old Dmitri Bonville using boys own stem cells, in what is believed to be first time ... Unlike the German procedure, which required a painful extraction of cells from the bone marrow, he extracted white blood cells ... Researchers working with animals at first assumed that injected bone marrow stem cells would turn into heart muscle cells, an ... Doctors Use Bone Marrow Stem Cells to Repair a Heart. By NICHOLAS WADE. MARCH 7, 2003. ...
David Steenblock of Mission Viejo, California, a pioneer in clinical applications of stem cells, is pleased to report the ... "Cerebral Palsy Improves After Bone Marrow Stem Cell Procedure." Medical News Today. MediLexicon, Intl., 9 Jul. 2009. Web.. 21 ... Medicine, P. (2009, July 9). "Cerebral Palsy Improves After Bone Marrow Stem Cell Procedure." Medical News Today. Retrieved ... Contained within a patients own bone marrow are stem cells, which when given back to the same person intravenously, can ...
Gifts including Bone Marrow Transplant Survivor & Donor Awareness shirts, clothing and gifts to encourage bone marrow donors. ... Sells unique Stem Cell Transplant Awareness & Survivor Shirts, Tees, Apparel & ...
"Weve known that bone marrow cells are involved in wound healing and inflammation - now we have data that shows bone marrow ... Bone marrow has been studied for a number of purposes in recent years because it is rich in stem cells - cells that can go on ... Researchers found green cells throughout the body, but observed that the highest concentration of bone marrow cells was in ... "What we have here is a new cell population that was not previously recognized," Isik said. "The bone marrow cells help form the ...
... kitPOS cells in the bone marrow and a redistribution of these cells from the bone marrow to the peripheral blood. This protocol ... bone marrow cells;. SCF,. stem cell factor;. G-CSF,. granulocyte-colony stimulating factor;. LV,. left ventricle;. EC,. ... It could be argued that cytokine treatment mobilized bone marrow stem cells and resident cardiac stem cells, which together ... kitPOS cells from the bone marrow to the peripheral blood (13). The number of circulating Lin− c-kitPOS cells increased 250- ...
BERKELEY - When stretched, a type of adult stem cell taken from bone marrow can be nudged towards becoming the type of tissue ... "For cartilage and bone, particularly at the joints, cells experience compression forces," said Li. "Stem cells seem to know the ... Researchers placed mesenchymal stem cells, extracted from bone marrow, onto a silicone membrane that was stretched ... Mesenchymal stem cells have the ability to turn into different types of connective tissue including bone, cartilage and muscle ...
in the bone marrow is shown. The number of KSL (. D. ) or CFU-C (. E. ) in bone marrow, blood, and spleen are shown. Data are ... CXCR2 expression in sinusoidal endothelial cells and the expression of 2 CXCR2 ligands, CXCL1 and CXCL2, in the bone marrow ... A resident population of dendritic cells (DCs) has been identified in murine bone marrow, but its contribution to the ... Ablation of cDCs was associated with an expansion of bone marrow endothelial cells and increased vascular permeability. ...
... the number of KSL cells in bone marrow (F) and spleen (G), the number of KSL-SLAM cells in bone marrow (H) and spleen (I), and ... Ablation of bone marrow DCs induces endothelial cell expansion and increased vascular permeability in the bone marrow. The ... Our data suggest that DCs regulate endothelial cell function in the bone marrow. The great majority of bone marrow DCs are in ... bone marrow DCs were identified as CX3CR1-GFPhi and MHC-IIhi Gr-1lo B220-/CD19- cells. As expected, bone marrow DCs expressed a ...
... reduced CXCR4 expression in bone marrow mononuclear cells and caused mobilization of progenitor cells out of the bone marrow. ... Inside those progenitor cells, the microRNAs turn off a specific gene that allows the progenitor cells to leave the bone marrow ... For 15 years, it had been known that progenitor cells are released from the bone marrow after a heart attack. These cells move ... These myo-miRs were preferentially taken up by bone marrow mononuclear cells, and to a lesser extent, kidney cells. MicroRNAs, ...
Nerve cells that have not merged with the insulin-producing bone marrow cells remain intact and function normally. "Based on ... "These insulin-producing bone marrow cells are like terrorists that infiltrate the nerve-cell populations," he said. They ... Previously, Chan and members of his laboratory had found that bone marrow cells were among a group of cells in organs other ... They found that, in diabetes, only nerve cells that have fused with bone marrow cells display the abnormal function and ...
Title : Small Molecule Protection of Bone Marrow Hematopoietic Stem Cells. Descriptive Note : Technical Report,30 Sep 2015,29 ... Descriptors : stem cells , bone marrow , HEMATOPOIETIC SYSTEM formaldehyde , aldehydes , mass spectrometry , dna ...
  • Cudkowicz G, Lotzova E (1973): Hemopoietic cell-defined components of the major histocompatibility complex of mice: Identification of responsive and unresponsive recipients for bone marrow transplants. (springer.com)
  • LSCs are defined as cells that (1) are capable of initiating the disease in an immunodeficient animal as well as sustaining the disease through multiple serial transplants (self-renewal) [ 22 , 23 ], and (2) give rise to the bulk of leukemic cells. (nature.com)
  • Bone marrow transplants work best if the HLAs from the donor and the patient are a close match. (medlineplus.gov)
  • More than 50,000 stem cell transplants are performed each year worldwide. (medicalnewstoday.com)
  • There are several types of bone marrow transplants, also referred to as stem-cell transplants. (emoryhealthcare.org)
  • In Sweden there is only one entirely new trial underway, and it can easily be discontinued, but in Germany and elsewhere a huge number of patients have already had blood stem cells transplanted into their heart, and more transplants are in planned. (eurekalert.org)
  • There are some indications that bone marrow transplants have a certain positive effect on the heart function after an infarction, but the mechanism behind this remains an open question. (eurekalert.org)
  • The team is actively involved in clinical research in bone marrow and peripheral blood stem cells transplants. (karmanos.org)
  • Our partnership with the Children's Hospital of Michigan at the Detroit Medical Center makes Karmanos an accredited center not only for the treatment of adults, but children of all ages who need bone marrow or stem cell transplants. (karmanos.org)
  • We perform both autologous (your own) and allogenic (from a related or unrelated donor) stem cell transplants at King's College Hospital. (guysandstthomas.nhs.uk)
  • Our care teams perform more than 200 stem cell transplants a year and we perform more allogeneic transplants (transplants using bone marrow or stem cells from donors) for older adults than any other center in the tri-state area. (nyp.org)
  • Umbilical Cord Blood Transplants -using stem cells from the umbilical cord of a newborn. (nyp.org)
  • Autologous Peripheral blood stem cell transplants (your stem cells are used). (nyp.org)
  • Options for individuals lacking a related donor include haplo-cord transplants -- a combination of donated umbilical cord blood stem cells and half-matched (haploidentical) cells from a related donor. (uchospitals.edu)
  • Lucy Godley, MD, PhD, talks about what stem cell transplants are like for the patient receiving the transplant, potential complications associated with the procedure, and the long-term prognosis after transplant. (uchospitals.edu)
  • Accurate patient information is vital to learn about the latest advancements in cancer treatments, including bone marrow transplants. (uhhospitals.org)
  • Since 1986, Cook Children's Bone Marrow and Stem Cell Transplant program has performed more than 1,000 transplants in children with cancer, blood disorders or inherited conditions. (cookchildrens.org)
  • At Cook Children's, we are committed to innovative clinical research, as well as advancing the field of stem cell transplants and improving patient outcomes. (cookchildrens.org)
  • Cook Children's Bone Marrow and Stem Cell Transplant Program has performed multiple autologous and allogeneic transplants, making it one of the more diverse and experienced pediatric transplant programs in the Southwest. (cookchildrens.org)
  • Remarkably, the researchers found that bone marrow or blood cell transplants appear to completely revive the ovaries of female mice sterilized by chemotherapy. (bio-medicine.org)
  • Although it is not yet clear whether the egg cells (oocytes) generated following bone marrow transplants can mature and be fertilized to give rise to viable mouse pups, the findings provide an important first step toward investigating such potential for restoring fertility. (bio-medicine.org)
  • About 200 children have been cured of sickle cell with transplants, but the procedure was considered too harsh for adults with severe sickle cell disease. (dailypress.com)
  • A sibling donor is normally necessary in sickle-cell transplants to ensure that the patient doesn't reject the new marrow. (dailypress.com)
  • But researchers have observed that bone marrow transplants in children with the disease -- and in patients with some other diseases -- can be successful even if all of the patient's own marrow is not destroyed. (dailypress.com)
  • Read about stem cell or bone marrow transplants, including why they're used, what's involved, and what the potential risks are. (axappphealthcare.co.uk)
  • Why are stem cell transplants carried out? (axappphealthcare.co.uk)
  • Stem cell transplants are used to treat conditions in which the bone marrow is damaged and is no longer able to produce healthy blood cells. (axappphealthcare.co.uk)
  • Transplants can also be carried out to replace blood cells that are damaged or destroyed as a result of intensive cancer treatment. (axappphealthcare.co.uk)
  • Stem cell transplants are complicated procedures with significant risks. (axappphealthcare.co.uk)
  • Bone marrow is the traditional source of stem cells for both autologous and homologous transplants. (medscape.com)
  • Matching a stem cell donor is typically a concern associated with allogeneic transplants, since these involve stem cell sources donated from either a family member, unrelated individual, or a cord blood unit from a cord blood bank . (wdxcyber.com)
  • In the case of autologous or syngeneic stem cell transplants, an HLA match is guaranteed since the stem cell source comes from either the patient or the patient's identical twin who carries identical genes. (wdxcyber.com)
  • HLA matching is a key factor when it comes to stem cell transplants, whether cord blood or bone marrow transplants. (wdxcyber.com)
  • The benefits of cord blood transplants over a bone marrow transplant when it comes to HLA matching lies in the fact that cord blood is more flexible in cases of an incomplete match. (wdxcyber.com)
  • Therefore, a 4/6 HLA match may be acceptable for stem cell transplants, while a minimum of a 5/6 HLA match is required for a bone marrow transplant. (wdxcyber.com)
  • When it comes to cord blood transplants , the number of stem cells found in a cord blood unit must be measured and selected in accordance to the patient's weight. (wdxcyber.com)
  • Most transplants these days use stem cells collected from the bloodstream. (bmtinfonet.org)
  • Bone Marrow Transplants (BMT) and Peripheral Blood Stem Cell Transplants (PBSCT) are emerging as mainstream treatment for many cancers, including Hodgkin's Disease and Medium/High grade aggressive)Non-Hodgkin's lymphoma. (lymphomainfo.net)
  • People with life-threatening diseases, such as leukemia , lymphoma , and myeloma can be treated with a bone marrow transplant . (medlineplus.gov)
  • This is now often called a stem cell transplant. (medlineplus.gov)
  • Autologous bone marrow transplant is when people donate their own bone marrow. (medlineplus.gov)
  • Allogenic bone marrow transplant is when another person donates bone marrow. (medlineplus.gov)
  • But only about 30% of people who need a bone marrow transplant can find a matching donor in their own family. (medlineplus.gov)
  • Doctors can then use the registry to find a matching donor for a person who needs a bone marrow transplant. (medlineplus.gov)
  • Stem cell transplant for cancer. (medlineplus.gov)
  • Finding a bone marrow donor match is challenging and the number of bone marrow cells from a single harvest procedure are often not sufficient for a transplant. (medicalnewstoday.com)
  • The immediate goal is for us to see if we can take fewer blood stem cells from a donor and expand them for transplant. (medicalnewstoday.com)
  • The hope is that when a patient needs a bone marrow transplant to treat cancer or another disease, we can find the cells that match, expand them and use them. (medicalnewstoday.com)
  • One treatment for patients with blood cancers produced by abnormal blood cells is to remove the unhealthy marrow and transplant healthy blood stem cells from a donor. (medicalnewstoday.com)
  • Patients with some cancers may also need a bone marrow transplant when anticancer treatments damage the blood. (medicalnewstoday.com)
  • Sells unique Stem Cell Transplant Awareness & Survivor Shirts, Tees, Apparel & Gifts including Bone Marrow Transplant Survivor & Donor Awareness shirts, clothing and gifts to encourage bone marrow donors. (cafepress.com)
  • The researchers removed bone marrow from the mice and then performed a stem cell transplant into a genetically identical strain of normal mice, whose cells do not glow green. (innovations-report.com)
  • Bone marrow transplant (BMT) can strengthen the body to fight cancer by replacing the blood building cells in the body that are destroyed by chemotherapy and radiation treatments, which are used to kill the cancer cells, with healthy stem cells found in bone marrow. (emoryhealthcare.org)
  • In a bone marrow transplant, the patient's diseased bone marrow is destroyed and healthy bone marrow stem cells are infused into the patient's blood-stream. (emoryhealthcare.org)
  • In a successful BMT transplant, the new bone marrow migrates to the cavities of the large bones and begins producing healthy, normal blood cells. (emoryhealthcare.org)
  • Here at Winship Cancer Institute Bone Marrow Transplant Center we believe patient information is a vital part of the transplant process. (emoryhealthcare.org)
  • Researchers found cells in the women's brains with the male, or "Y", chromosome, essentially meaning the women had transgender brain tissue.In the woman who survived the longest after the transplant, evidence of the male cells was present in nerve cells as well. (medindia.net)
  • In this study, the team performed a bone marrow (with vitamin D receptors) transplant for a mouse model without vitamin D receptors (this means it has a high concentration of vitamin D in the body) to create a myelofibrosis model. (news-medical.net)
  • Professor Katayama comments: 'The only permanent cure for this disease is hematopoietic stem cell transplant, but this method is unsuitable for many elderly patients. (news-medical.net)
  • If you're in need of a bone marrow or stem cell transplant, it's important to be evaluated by the experts in this field before beginning treatment. (karmanos.org)
  • Karmanos' Bone Marrow & Stem Cell Transplant Multidisciplinary Team includes specialists in medical hematology and oncology, infectious disease, immunotherapy, pediatrics and internal medicine who bring years of experience to the field of transplantation. (karmanos.org)
  • This survival data is reported yearly from the Center for International Blood and Marrow Transplant Registry for all 168 transplant centers in the U.S. The data are adjusted for several risk factors that may have a major influence on outcome. (karmanos.org)
  • In addition, our program has received full accreditation from the Foundation for the Accreditation of Cellular Therapy (FACT) - the highest level and the most extensive spectrum of accreditation offered by FACT for Bone Marrow Transplant Programs. (karmanos.org)
  • Members of the Bone Marrow Transplant Team have vast experience in clinical research focusing on graft-versus-host disease and immunotherapy. (karmanos.org)
  • If you have had a bone marrow transplant, you will need to stay in hospital for around four to six weeks. (guysandstthomas.nhs.uk)
  • At NewYork-Presbyterian, bone marrow transplant candidates benefit from the world-class care and experience, in a compassionate environment. (nyp.org)
  • We have advanced, dedicated bone marrow transplant units offering personal monitoring and special airflow systems for patients with weakened immune systems. (nyp.org)
  • You may have the opportunity to participate in a clinical trial studying new, safer methods of preparing for, receiving, and recovering from a bone marrow transplant. (nyp.org)
  • Our transplant programs offer access to highly innovative cellular treatment protocols including gene therapeutic approaches (for sickle cell disease) or cellular immunotherapy for treatment or prevention of viral diseases. (nyp.org)
  • Stem cell transplant is a complex and often-arduous process for both the patient and his or her caregivers. (nyp.org)
  • Stem cell transplant (also known as bone marrow transplant or BMT) is an established treatment for many cancers and blood diseases once considered incurable. (uchospitals.edu)
  • Ongoing advances in stem cell transplant are expanding its availability and improving outcomes for patients, young and old. (uchospitals.edu)
  • Here at the University of Chicago Medicine, the brightest minds in medicine are ready to meet the needs of all patients considering a stem cell transplant. (uchospitals.edu)
  • We offer the latest promising approaches in blood and bone marrow stem cell transplant. (uchospitals.edu)
  • Recognizing the distinct needs of this population, we've assembled a multidisciplinary team of stem cell transplant and geriatric oncology experts to design a care program tailored specifically for patients over 60. (uchospitals.edu)
  • The Stem Cell Transplant Unit, located on the top floor of the Center for Care and Discovery, offers the newest technology as well as many thoughtful patient and family amenities. (uchospitals.edu)
  • The unit integrates both inpatient and outpatient stem cell transplant care services in one convenient location. (uchospitals.edu)
  • This groundbreaking work influenced many scientists investigating bone marrow transplant for humans, including the winner of the 1990 Nobel Prize in Physiology or Medicine. (uchospitals.edu)
  • For information about stem cell transplant for children and teens, visit the Pediatric Stem Cell Transplant page on the University of Chicago Comer Children's Hospital website. (uchospitals.edu)
  • When this happens, your child may require a very complex process called a stem cell or bone marrow transplant. (cookchildrens.org)
  • The Children's Oncology Group (COG ) stem cell transplant section. (cookchildrens.org)
  • Stem cells that come from an individual patient for their own use (autologus transplant) are collected from the blood through a special IV catheter after receiving chemotherapy. (cookchildrens.org)
  • These cells are frozen before the child or teen undergoes a transplant. (cookchildrens.org)
  • Just 24 hours after a transplant, the sterilized mice had new egg cells and follicles, the nurturing group of cells that encloses each egg cell. (bio-medicine.org)
  • Two months after bone marrow transplant, the ovaries of normal mice and mice that had undergone chemotherapy appeared nearly identical, Tilly and colleagues discovered. (bio-medicine.org)
  • A bone marrow or stem cell transplant is a procedure to remove stem cells from blood or bone marrow. (drugs.com)
  • You may need several days of tests to make sure your body is ready for stem cell transplant. (drugs.com)
  • If you have an autologous stem cell transplant, you will have your own blood or bone marrow removed from your body about 2 weeks before your transplant. (drugs.com)
  • Before a stem cell transplant, you may receive cancer treatment, such as chemo or radiation. (drugs.com)
  • You will be monitored very closely after stem cell transplant. (drugs.com)
  • You may also need medicines to treat side effects from the stem cell transplant. (drugs.com)
  • If you get an autologous stem cell transplant, it may contain cancer cells, and the cancer may spread to other parts of your body. (drugs.com)
  • Listed below are our archive of questions and answers from The Bone Marrow Transplant Forum for the medical topic: Bone Marrow Transplant. (medhelp.org)
  • Please feel free to browse our Bone Marrow Transplant archive below, search our site for additional information about bone marrow transplant, or browse the full archives for this forum. (medhelp.org)
  • Researchers have for the first time performed a successful bone marrow transplant to cure sickle cell disease in adults, a feat that could expand the procedure to more of the 70,000 Americans with the disease -- and possibly some other diseases as well. (dailypress.com)
  • The researchers developed a new regimen using about a quarter of the radiation dose normally required for a bone marrow transplant and smaller quantities of drugs to kill bone marrow cells. (dailypress.com)
  • Because not all of the host marrow cells are killed, the patient retains some immunity and does not have to be kept in a germ-free environment in the hospital for as long as they would with a conventional transplant. (dailypress.com)
  • Thousands of people with blood cancers and other diseases - like sickle cell anemia - need a marrow or blood stem cell transplant to survive. (ne.gov)
  • 70% of those needing a marrow transplant don't have a fully-matched donor in their family. (ne.gov)
  • A blood stem cell transplant replaces a person's unhealthy blood-forming cells with healthy ones. (ne.gov)
  • A stem cell or bone marrow transplant replaces damaged blood cells with healthy ones. (axappphealthcare.co.uk)
  • A stem cell transplant involves destroying any unhealthy blood cells and replacing them with stem cells removed from the blood or bone marrow. (axappphealthcare.co.uk)
  • A stem cell transplant will usually only be carried out if other treatments haven't helped, the potential benefits of a transplant outweigh the risks and you're in relatively good health, despite your underlying condition. (axappphealthcare.co.uk)
  • What does a stem cell transplant involve? (axappphealthcare.co.uk)
  • A stem cell transplant can involve taking healthy stem cells from the blood or bone marrow of one person - ideally a close family member with the same or similar tissue type (see below) - and transferring them to another person. (axappphealthcare.co.uk)
  • It's also possible to remove stem cells from your own body and transplant them later, after any damaged or diseased cells have been removed. (axappphealthcare.co.uk)
  • A stem cell transplant has 5 main stages. (axappphealthcare.co.uk)
  • Having a stem cell transplant can be an intensive and challenging experience. (axappphealthcare.co.uk)
  • Read more about what happens during a stem cell transplant ↗ . (axappphealthcare.co.uk)
  • The history of stem cell transplant goes back as early as 1939, when the first documented clinical transplant was performed. (medscape.com)
  • In 1968, the first successful allogenic stem cell transplant was made possible followed by series of achievements in 1970s and 1980s. (medscape.com)
  • Donor lymphocyte infusion (DLI) has a significant role in relapsed patients with chronic myelogenous leukemia (CML) after stem cell transplant. (medscape.com)
  • The success of collection of mobilized stem cells from peripheral blood was a milestone in the history of transplant. (medscape.com)
  • Stem cell donor and HLA matching are integral to improving the success of a transplant, to promoting engraftment or the growth of new cells, and to preventing transplant rejection, also known as GVHD. (wdxcyber.com)
  • There are three antigen groups that are considered important when it comes to matching for a stem cell transplant. (wdxcyber.com)
  • It is the responsibility of a transplant center to find a stem cell donor for a patient, and not the individual. (wdxcyber.com)
  • A doctor may perform a preliminary search for a donor without obliging the patient to necessarily undergo a stem cell transplant. (wdxcyber.com)
  • This search is completely free of charge, and further analysis would be necessary should a stem cell transplant treatment be chosen over other options. (wdxcyber.com)
  • If a donor match cannot be found, your doctor may recommend stem cell transplant alternatives. (wdxcyber.com)
  • Many stem cell transplant centers will look at more than the crucial six HLA types of antigens in order to a select a donor and insure an ideal match. (wdxcyber.com)
  • The procedure to collect blood stem cells for your transplant is called a bone marrow harvest or peripheral blood stem cell harvest. (bmtinfonet.org)
  • If you are providing the blood stem cells for a transplant, they will either be collected from your bloodstream (peripheral blood) or from your bone marrow. (bmtinfonet.org)
  • If you are collecting stem cells for your own transplant, chemotherapy drugs may be used to help move the stem cells out of your bone marrow into the bloodstream. (bmtinfonet.org)
  • It can take one to three days to collect enough stem cells for transplant. (bmtinfonet.org)
  • The procedure used to collect bone marrow for transplant is called a bone marrow harvest. (bmtinfonet.org)
  • Several skin and bone punctures are required to extract sufficient bone marrow for transplant. (bmtinfonet.org)
  • GVHD is a common problem after a transplant using donor cells. (bmtinfonet.org)
  • If the transplanted marrow is from another person, it is called an allogeneic transplant. (lymphomainfo.net)
  • In PBSCTs, another type of autologous transplant, the patient's blood is passed through a machine that removes the stem cells - the immature cells from which all blood cells develop. (lymphomainfo.net)
  • DKMS connects its donors with patients around the world in need of a bone marrow transplant. (lymphomainfo.net)
  • The researchers then took bone marrow from participants' hips and extracted the mass of mononuclear cells-an ill-defined mix of stem cells and progenitor cells. (technologyreview.com)
  • These myo-miRs were preferentially taken up by bone marrow mononuclear cells, and to a lesser extent, kidney cells. (infowars.com)
  • The researchers found that both the myo-miR exosomes, from after heart attacks, and the non-heart-attack exosomes readily transferred into bone marrow mononuclear cells. (infowars.com)
  • Since one of the myo-miRs showed a potential binding to the CXCR4 mRNA, all four of the myo-miRs were tested on bone marrow mononuclear cells. (infowars.com)
  • Furthermore, exosomes from heart-attack mice - but not from non-heart-attack mice - downregulated CXCR4 in bone marrow mononuclear cells. (infowars.com)
  • Finally, researchers showed that exosomes from heart-attack mice, when transferred to intact mice, significantly reduced CXCR4 expression in bone marrow mononuclear cells and caused mobilization of progenitor cells out of the bone marrow. (infowars.com)
  • After precipitation of red blood cells, Low density mononuclear cells will be collected by centrifugation in Ficoll-Paque density gradient. (clinicaltrials.gov)
  • Primary human CD105+ cells were isolated from bone marrow (BM) mononuclear cells (MNCs) using positive immunomagnetic separation techniques. (stemcell.com)
  • Human primary stromal cells (mesenchymal stem and progenitor cells) are produced by expanding bone marrow mononuclear cells (MNCs) in culture and cryopreserved following the first passage in culture. (stemcell.com)
  • Normal bone marrow mononuclear cells were seeded at 1x10 6 cells/ml in 4 different lots of MarrowMAX™ medium. (thermofisher.com)
  • Percentage of functioning grafts in patients with implantation of autologous bone marrow mononuclear cells. (clinicaltrials.gov)
  • The purpose of this study evaluate the effect of the method of administration of autologous bone marrow mononuclear cells for the duration of of functioning aorto-coronary bypass grafts in the surgical treatment of coronary heart disease, to assess the degree of effectiveness depending on the method of transplantation (intramyocardial, intracoronary). (clinicaltrials.gov)
  • After centrifugation, the band of bone marrow mononuclear cells (BMMC) at the interface was removed using a pipette. (protocol-online.org)
  • Haematopoietic stem and progenitor cells differentiate to generate all major blood cell lineages and are supported by the bone marrow stroma. (nature.com)
  • One theory is that a type of progenitor cell called a mesenchymal cell, which gives rise to muscle and bone tissue, might encourage the growth of new blood vessels by releasing growth factors. (technologyreview.com)
  • The origin of fibroblasts in pulmonary fibrosis is assumed to be intrapulmonary, but their extrapulmonary origin and especially derivation from bone marrow (BM) progenitor cells has not been ruled out. (jci.org)
  • Thus the collagen-producing lung fibroblasts in pulmonary fibrosis can also be derived from BM progenitor cells. (jci.org)
  • The bone marrow (BM) is an invaluable source of adult pluripotent stem cells, including hematopoietic stem cells (HSCs), endothelial progenitor cells (EPCs), and mesenchymal stem cells (MSCs). (hindawi.com)
  • A method is described for generating a clinically significant volume of neural progenitor cells from whole bone marrow. (google.com)
  • Further methods of the present invention are directed to utilizing the neural progenitor cells cultured in this fashion in the treatment of various neuropathological conditions, and in targeting delivery of cells transfected with a particular gene to diseased or damaged tissue. (google.com)
  • 2. The neural progenitor cells of claim 1 , wherein the medium further comprises a supplement selected from the group consisting of B27, N2, and combinations thereof. (google.com)
  • 3. The neural progenitor cells of claim 1 , wherein the medium further comprises an additional compound selected from the group consisting of interleukin-3 (IL-3), stem cell factor-1 (SCF-1), sonic hedgehog (Shh), fms-like tyrosine kinase-3 (Flt3) ligand, leukemia inhibitory factor (LIF), and combinations thereof. (google.com)
  • Embodiments of the present invention are directed to a method for generating a clinically substantial volume of neural progenitor cells from mammalian whole bone marrow. (google.com)
  • Further embodiments of the present invention are directed to the treatment of neurological disorders using neural progenitor cells cultured in this fashion. (google.com)
  • progenitor cells, in turn, proliferate into the differentiated cells that populate the body. (google.com)
  • Certain maturation-promoting polypeptides cause the myeloid stem cell to differentiate into precursor cells, which in turn differentiate into various progenitor cells. (google.com)
  • It is the progenitor cells that proliferate into the various lymphocytes, neutrophils, macrophages, and other cells that comprise blood tissue of the body. (google.com)
  • Factors secreted by endothelial progenitor cells enhance neurorepair responses after cerebral ischemia in mice," PLoS ONE , vol. 8, no. 9, Article ID e73244, 2013. (hindawi.com)
  • Ablation of conventional DCs (cDCs) results in hematopoietic stem/progenitor cell (HSPC) mobilization that was greater than that seen with ablation of bone marrow macrophages, and cDC ablation also synergizes with granulocyte-colony stimulating factor to mobilize HSPCs. (jci.org)
  • These exosomes preferentially carry the microRNAs to progenitor cells in the bone marrow. (infowars.com)
  • Inside those progenitor cells, the microRNAs turn off a specific gene that allows the progenitor cells to leave the bone marrow and enter the bloodstream. (infowars.com)
  • For 15 years, it had been known that progenitor cells are released from the bone marrow after a heart attack. (infowars.com)
  • The UAB and Chinese researchers have now linked those two observations by identifying the cargo carrier that ferries those heart-muscle microRNAs, locating the preferred destination of the cargo carrier and describing the microRNA mechanism that releases the progenitor cells from the bone marrow. (infowars.com)
  • It was known that blockade of a chemokine receptor called CXCR4 mediates mobilization of bone marrow progenitor cells into the blood circulation and contributes to repair of heart muscle after heart attacks. (infowars.com)
  • Thus, overall, the injured heart is signaling to mobilize the bone marrow progenitor cells. (infowars.com)
  • Our work reveals that exosomes released from the ischemic heart are in fact mediating the systemic response of bone marrow progenitor cells to the site of injury," the researchers reported. (infowars.com)
  • Several studies in recent years suggest that bone marrow derived stem cells such as mesenchymal stem cells, progenitor cells such as endothelial progenitor cells and fibrocytes may be involved in these processes, contributing to skin cells or releasing regulatory cytokines. (nih.gov)
  • Stem/progenitor cells may be mobilized to leave the bone marrow, home to injured tissues and participate in the repair and regeneration. (nih.gov)
  • Direct injection of bone marrow derived mesenchymal stem cells or endothelial progenitor cells into injured tissues shows improved repair through mechanisms of differentiation and/or release of paracrine factors. (nih.gov)
  • The new study suggests an unexpected source for the progenitor cells t. (bio-medicine.org)
  • The new study suggests an unexpected source for the progenitor cells that can jumpstart new egg cell production--outside of the ovary--say Jonathan Tilly and colleagues at Massachusetts General Hospital and Harvard Medical School. (bio-medicine.org)
  • Further results suggest that the ovary itself is sending out a chemical signal to the bone marrow, readying the progenitor cells to travel to the ovary and restock its egg cell supply. (bio-medicine.org)
  • It could ultimately be possible, they continued, to inject these stem cells into patients, or to induce the patient's own stem cells to differentiate into progenitor cells capable of arterial repair, to forestall or even prevent the development of atherosclerosis, a disease process that causes arteries to clog and become less elastic. (brightsurf.com)
  • As part of their continuing effort to skip over the ethical and political hurdles surrounding embryonic stem cells, researchers have unlocked even more potential from the adult kind. (scientificamerican.com)
  • After transplantation of bone marrow (BM) hematopoietic stem cells or nonhematopoietic mesenchymal stem cells, muscle ( 2 ), heart ( 3 ), liver ( 4 ), and lung ( 5 , 6 ) cells of donor origin have been detected. (jci.org)
  • For this type of treatment, bone marrow is collected from a donor. (medlineplus.gov)
  • Then, a counselor meets with the donor to discuss the bone marrow donation process. (medlineplus.gov)
  • Donor stem cells can be collected in two ways. (medlineplus.gov)
  • Most donor stem cells are collected through a process called leukapheresis. (medlineplus.gov)
  • First, the donor is given 5 days of shots to help stem cells move from the bone marrow into the blood. (medlineplus.gov)
  • The red blood cells are returned to the donor through an IV in the other arm. (medlineplus.gov)
  • After a bone marrow harvest, the donor stays in the hospital until they're fully awake and can eat and drink. (medlineplus.gov)
  • In the journal Blood, Weill Cornell researchers and collaborators from Memorial-Sloan Kettering Cancer Center describe how they engineered a protein to amplify adult HSCs once they were extracted from the bone marrow of a donor. (medicalnewstoday.com)
  • A donor may also need to undergo the procedure multiple times in order to provide enough stem cells for the recipient. (medicalnewstoday.com)
  • Because of these issues of extracting donor bone marrow, there have been a number of attempts to expand HSCs that have focused on the transcription factor HOXB4, which stimulates HSCs to make copies of themselves. (medicalnewstoday.com)
  • Cyclophosphamide and total body irradiation (TBI), Corynebacterium parvum and TBI, a 3- or 5-day delayed transfusion of bone marrow cells after TBI, or an increase in the number of donor bone marrow cells or lymphocytes appeared to be ineffective. (osti.gov)
  • Prolonged survival was noted after transplantation of bone marrow cells from a one-DLA haplotype-mismatched donor. (osti.gov)
  • Stem cell harvesting and donor assessments are carried out at the haematology day unit. (guysandstthomas.nhs.uk)
  • Adult Donor Allogeneic transplantation (a donor's stem cells are used, collected from either the blood of from the bone marrow). (nyp.org)
  • NewYork-Presbyterian participates in the National Marrow Donor Program providing us access to all available donors worldwide and enhancing your chances of finding an appropriate donor. (nyp.org)
  • If you are a candidate for bone marrow transplantation but do not have a matching donor, you may benefit from our experience performing this procedure using mismatched donors. (nyp.org)
  • Partial matches" have made bone marrow transplantation a possibility for people who may have a challenging time finding a matched donor, such as those from ethnic minority groups. (nyp.org)
  • Hematopoietic stem cells may come from the patient or from a donor. (cookchildrens.org)
  • Donor stem cells come from a compatible family member or through a match from a worldwide registry of donors. (cookchildrens.org)
  • Donor-derived bone marrow cells have been identified in human uterine endometrium. (wiley.com)
  • After bone marrow transplantation, male donor-derived bone marrow cells were found in the uterine endometrium of female mice. (wiley.com)
  • The main problem is that not enough sickle cell patients have a healthy sibling who is a compatible donor. (dailypress.com)
  • A key feature of the regimen was the use of the immunosuppressive drug sirolimus (formerly known as rapamycin) to prevent rejection of the donor cells. (dailypress.com)
  • Considering becoming a bone marrow or a blood stem cell donor? (cancer.gov)
  • An image depicting bone marrow donor typing procedures can be seen below. (medscape.com)
  • The eligibility criteria for a stem cell donor are essentially the same as for blood donation. (medscape.com)
  • Most bone marrow donor procedures are performed under general anesthesia. (medscape.com)
  • This makes it more likely for a sibling to be a potential stem cell donor, with a 25% chance of inheriting the same antigens from the parents. (wdxcyber.com)
  • It can take as little as two weeks to find a cord blood unit match, while several months may be required to locate a bone marrow donor. (wdxcyber.com)
  • Using specific staining techniques on the aortas, the researchers were able to determine that the donor stem cells "homed in" on areas where atherosclerotic lesions are most common, especially where smaller vessels branch off from larger vessels. (brightsurf.com)
  • To further prove that the donor stem cells were responsible for rejuvenating arteries, the scientists measured the lengths of structures at the end of chromosomes known as telomeres of the endothelial cells and found that they were longer in the treated mice than the untreated mice. (brightsurf.com)
  • DKMS Bone Marrow Donor Center is a non-profit organization dedicated to fighting leukemia by registering bone marrow donors from every race and ethnicity. (lymphomainfo.net)
  • Hematopoietic stem cells can be found in bone marrow (the spongy tissue inside bones), the bloodstream, or the umbilical cord blood of newborn babies. (cookchildrens.org)
  • Nov. 26 (UPI) -- Breast cancer tumors can grow by recruiting other cells from bone marrow, lowering the chances of a patient's survival, a new study says. (upi.com)
  • Bone marrow stem cells used in the study were taken from the top of the patient's pelvis and sorted in the lab before being injected back into the heart area, where they improved ventricular function, or the heart's ability to pump blood. (news24.com)
  • Contained within a patient's own bone marrow are stem cells, which when given back to the same person intravenously, can migrate to injured areas and repair tissue(s) that are damaged, even if the damage occurred over sixteen years ago. (medicalnewstoday.com)
  • The cells are obtained by inserting a needle into the patient's hip bone. (blindness.org)
  • The stem cells are then injected into the patient's eye. (blindness.org)
  • These stem cells are then thawed and put back into the patient's body after high-dose chemotherapy treatment is complete. (cookchildrens.org)
  • While all three types of blood cells can replenish a patient's blood and bone marrow cells, there are advantages and disadvantages to each. (cookchildrens.org)
  • Fibrin glue created from a patient's own blood can be used as a carrier to deliver cells to the specific site of an injury. (hindawi.com)
  • Fibrin glue derived from the patient's own blood can be used as a carrier to adhere the cells in the damaged region [ 17 , 21 ]. (hindawi.com)
  • This is because umbilical cord blood contains immature stem cells that are less likely to attack a patient's immune system. (wdxcyber.com)
  • When high doses of chemotherapy are planned, which can destroy the patient's bone marrow, physicians will typically remove marrow from the patient's bone before treatment and freeze it. (lymphomainfo.net)
  • Furthermore, the bone marrow-derived NSC differentiate in vivo into functional oligodendrocytes and neurons following demyelinating lesions, thus, demonstrating the ability of adult bone marrow progenitors to generate self-renewing, functional neural stem cells, validating this approach as an alternative source of long-lasting neural stem cells with therapeutic implications in neurodegenerative diseases. (nih.gov)
  • Stem cells in the adult have traditionally been thought to be restricted in their potential to differentiate and regenerate tissues in which they reside. (jci.org)
  • MSCs not only differentiate into types of cells of mesodermal lineage but also into endodermal and ectodermal lineages such as bone, fat, cartilage and cardiomyocytes, endothelial cells, lung epithelial cells, hepatocytes, neurons, and pancreatic islets. (hindawi.com)
  • The engineered protein maintains the expanded HSCs in a stem-like state - meaning, they will not differentiate into specialized blood cell types before they are transplanted in the recipient's bone marrow. (medicalnewstoday.com)
  • Adult bone marrow stromal cells differentiate into neural cells in vitro," Experimental Neurology , vol. 164, no. 2, pp. 247-256, 2000. (hindawi.com)
  • On this basis, we hypothesized here that BMC, mobilized by stem cell factor and granulocyte-colony stimulating factor, would home to the infarcted region, replicate, differentiate, and ultimately promote myocardial repair. (pnas.org)
  • Publication date: Available online 17 July 2019Source: Biochimica et Biophysica Acta (BBA) - Gene Regulatory MechanismsAuthor(s): Adam M. Heck, Carol J. WiluszAbstractIn order to maintain a state of self-renewal, yet retain the ability to rapidly differentiate in response to external signals, pluripotent cells exert tight control over gene expression at many levels. (medworm.com)
  • The potential for stem cells in bone marrow (BM) to differentiate into hepatocytes cells was recently confirmed. (clinicaltrials.gov)
  • Human bone marrow stromal cells derived in ACF medium (Catalog #70071) using the MesenCult™-ACF Culture Kit (Catalog #05449) differentiate to A) adipocytes (Oil Red O staining), B) chondrocytes (Alcian Blue and Nuclear Fast Red staining) and C) osteoblasts (Alizarin Red S staining). (stemcell.com)
  • Initially arising within the central nervous system, NC cells subsequently undergo an epithelial to mesenchymal transition to migrate into the periphery, where they differentiate into diverse cell types. (stemcell.com)
  • Bone marrow-derived cells (BMDCs) can differentiate into nonhematopoietic cells, suggesting that BMDCs may contribute to the maintenance of multiple tissues. (wiley.com)
  • 0.01%), these cells can differentiate into epithelial cells. (wiley.com)
  • Bone Mesenchymal stem cells (BMSCs) are derived from bone marrow, which exhibited a fibroblast-like appearance, and could differentiate in vitro into different lineages. (scirp.org)
  • In addition to their ability to differentiate into multiple different cell types that would be contributory to the recovery and repair of the brain by replacing destroyed cells, mesenchymal stem cells also secrete angiogenins, cytokines and trophic factors that can support and stimulate multiple other cell types. (clinicaltrials.gov)
  • Perhaps not surprisingly, the phenomenon of acute parental marrow graft rejection in F1 hybrid mice, also called hybrid resistance (HR), found its counterpart in the ability of irradiated mice to acutely reject allogeneic marrow grafts (Cudkowicz and Bennett, 1971a). (springer.com)
  • Again, the specificity of acute allogeneic marrow graft rejection pointed to antigenic determinants that were encoded close to the MHC region. (springer.com)
  • Cudkowicz G (1975a): Genetic control of resistance to allogeneic and xenogenic bone marrow grafts in mice. (springer.com)
  • Allogeneic hematopoietic cell transplantation (HCT) is a potential cure for patients with acute myeloid leukemia (AML). (nature.com)
  • The noted discrepancy between studies with mice and dogs invalidated allogeneic resistance as measured in the mouse spleen assay as a model for bone marrow allograft rejection. (osti.gov)
  • 10 This property has already been exploited in the clinical setting and has proved of therapeutic usefulness for the treatment of graft-versus-host disease after allogeneic bone marrow transplantation. (bloodjournal.org)
  • The purpose of this study is to assess the safety and tolerability of allogeneic adult mesenchymal bone marrow cells administered intravenously to patients with ischemic stroke. (clinicaltrials.gov)
  • Allogeneic mesenchymal stem cells have been used in a number of clinical trials for different indications demonstrated the safety of allogeneic mesenchymal stem cell treatment. (clinicaltrials.gov)
  • Mesenchymal stem cells (MSCs) are prototypical adult stem cells with the capacity for self-renewal and differentiation with a broad tissue distribution. (hindawi.com)
  • MSCs have been identified as an adherent, fibroblast-like population and can be isolated from different adult tissues, including bone marrow (BM), umbilical cord, skeletal muscle, and adipose tissue. (hindawi.com)
  • A study published in the Journal of Experimental Medicine found that cells found in breast cancer tumors can strengthen themselves by pulling in non-cancerous fibroblasts originally formed in bone marrow called mesenchymal stromal cells, or MSCs. (upi.com)
  • Neta Erez and colleagues at the Sackler School of Medicine, Tel Aviv University, discovered that, in mice with breast cancer, a significant number of cancer-associated fibroblasts are derived from bone marrow cells called mesenchymal stromal cells (MSCs). (medindia.net)
  • The researchers found that breast tumors can recruit MSCs from the bone marrow and cause them to develop into fibroblasts. (medindia.net)
  • OBJECTIVE- Mesenchymal stem cells (MSCs) have been reported to secrete various cytokines that exhibit angiogenic and neurosupportive effects. (diabetesjournals.org)
  • RESEARCH DESIGN AND METHODS- MSCs were isolated from bone marrow of adult rats and transplanted into hind limb skeletal muscles of rats with an 8-week duration of streptozotocin (STZ)-induced diabetes or age-matched normal rats by unilateral intramuscular injection. (diabetesjournals.org)
  • It has been shown that mesenchymal stem cells (MSCs) induce T cells to become unresponsive. (bloodjournal.org)
  • We characterized the phenotype of these T cells by dissecting the effect of MSCs on T-cell activation, proliferation, and effector function. (bloodjournal.org)
  • The inhibitory effect of MSCs was directed mainly at the level of cell proliferation. (bloodjournal.org)
  • Analysis of the cell cycle showed that T cells, stimulated in the presence of MSCs, were arrested at the G 1 phase. (bloodjournal.org)
  • When MSCs were removed from the cultures and restimulated with the cognate peptide, T cells produced IFN-γ but failed to proliferate. (bloodjournal.org)
  • MSCs did not preferentially target any T-cell subset, and the inhibition was also extended to B cells. (bloodjournal.org)
  • In adult life, stem cells of mesenchymal lineage (MSCs) are mainly confined to the bone marrow, where they constitute a small proportion (0.1%-0.01%) of the general cell population. (bloodjournal.org)
  • Further interest in their clinical application has been raised by the observation that MSCs from various species can exert profound immunosuppression by inhibiting T-cell responses to polyclonal stimuli 8 , 9 and to their cognate peptide. (bloodjournal.org)
  • Although the use of different methods and different species to generate MSCs has produced conflicting results, overall data suggest that soluble factors 9 , 13 - 15 and mechanisms mediated by cell contact 10 , 12 - 15 are involved. (bloodjournal.org)
  • IDO has been shown to be produced by MSCs stimulated by the proinflammatory cytokines released during T-cell activation. (bloodjournal.org)
  • 15 The second, but not mutually exclusive, mechanism for MSC-induced inhibition also implies an indirect effect because it would involve CD8 + cells with suppressive properties generated in vitro after contact with MSCs. (bloodjournal.org)
  • MSCs have been shown to inhibit T-cell proliferation in response to a variety of stimuli. (bloodjournal.org)
  • The aim of our study was to investigate the proliferation effect of 635 nm red laser light on bone marrow MSCs with or without osteogenic supplements. (scirp.org)
  • LLLI can be used as a efficiently tool for the preconditioning of bone marrow mesenchymal stem cells (MSCs), which derived from bone marrow and received wildly attentions in regeneration medicine. (scirp.org)
  • Therefore, it's important to investigate the proliferation effect of 638 nm red laser light on bone marrow MSCs with or without osteogenic supplements. (scirp.org)
  • The regeneration potential of mesenchymal stem cells (MSCs) diminishes with advanced age and this diminished potential is associated with changes in cellular functions. (biomedsearch.com)
  • To gain a better understanding of the regulation of cellular differentiation by mechanical cues, we investigated the influence of matrix stiffness ( E = 1.46 kPa and E = 26.12 kPa) on differentiated osteogenic cell lineage of bone marrow stem cells (BM-MSCs) and bone-derived cells (BDCs) using flexible collagen-coated polyacrylamide substrates. (wiley.com)
  • Cells in this population have been identified as CAR (CXCL12-abundant reticular) cells using Cxcl12-gfp reporter mice, Nestin-GFP dim cells using Nestin-gfp reporter mice and LepR + cells using LepR-cre mice 1 . (nature.com)
  • Researchers based in Louisiana showed that therapy with human multipotent stromal cells (hMSC) isolated from bone marrow was able to significantly reduce acute lung injury in mice after 48 hours. (redorbit.com)
  • Prior research with cultured tissue had shown that a mix of chemicals could change bone marrow stem cells from mice to those resembling brain cells, but when a team led by neurologist Lorraine Iacovitti of Thomas Jefferson University in Philadelphia tried the same brew on human cells, the number altered was modest. (scientificamerican.com)
  • Almost three decades ago, G. Cudkowicz (Cudkowicz and Cosgrove, 1961) made the curious observation that irradiated (A × B)F1 hybrid mice transplanted with parental bone marrow from either parent A or parent B may acutely reject one but not the other graft. (springer.com)
  • For example, the resistance of (C57BL/6 × C3H)F1 mice to C57BL/ 6 marrow was mapped to the D region of the MHC (Cudkowicz and Stimpfling, 1964b). (springer.com)
  • Cud-kowicz, 1975a), and the rejection of H-2 k marrow by strain 129 mice maps to the K region (Cudkowicz and Warner, 1979). (springer.com)
  • Cibotti R, Churaqui E, Bolonaki-Tsilivakos D, ScottAlgara O, Halle P, Kosmatopoulos K (1989): Specific inhibition of hybrid resistance in F1 hybrid mice pretreated with parent-strain spleen cells. (springer.com)
  • Cudkowicz G (1975b): Rejection of bone marrow allo-grafts by irradiated athymic nude mice. (springer.com)
  • Cudkowicz G, Cosgrove GE (1961): Modified homologous disease following transplantation of parental bone marrow and recipient liver into irradiated FI mice. (springer.com)
  • Cudkowicz G, Stimpfling JH (1964a): Deficient growth of C57BL marrow cells transplanted in F1 hybrid mice. (springer.com)
  • Cudkowicz G, Warner JF (1979): Natural resistance of irradiated 129-strain mice to bone marrow allografts: Genetic control by the H-2 region. (springer.com)
  • Induction of pulmonary fibrosis in such chimera mice by endotracheal bleomycin (BLM) injection caused large numbers of GFP + cells to appear in active fibrotic lesions, while only a few GFP + cells could be identified in control lungs. (jci.org)
  • Flow-cytometric analysis of lung cells confirmed the BLM-induced increase in GFP + cells in chimera mice and revealed a significant increase in GFP + cells that also express type I collagen. (jci.org)
  • These fibroblasts in the mice also lack PDGFRα, an important cell signaling protein that many other cancer-linked fibroblasts contain. (upi.com)
  • Afterward, only the bone marrow of the transplanted mice glowed green inside the bodies of the mice, allowing researchers to track the bone marrow cells throughout the body. (innovations-report.com)
  • The new cells identified in the transplanted mice did not produce that protein, and do not seem to be implicated in contact dermatitis. (innovations-report.com)
  • To test this possibility, mice were injected with SCF and G-CSF to increase the number of circulating stem cells from 29 in nontreated controls to 7,200 in cytokine-treated mice ( 13 ). (pnas.org)
  • Shown are the number of cDCs in the bone marrow, blood, and spleen ( n = 5, 4, 4 and 6 mice per time point). (jci.org)
  • These mice presented similar symptoms to myelofibrosis patients, displaying both fibrosis and bone hardening. (news-medical.net)
  • They treated the mice by rearing them on a low vitamin D diet, blocking vitamin D receptor signals (removing the vitamin D receptor gene in blood cells) and suppressing macrophages. (news-medical.net)
  • Bone marrow cell suspensions were prepared from male B6C3F1-mice and cultured with hydroquinone (123319), benzoquinone (106514), catechol (120809), phenol (108952), and 1,2,4-benzenetriol (533733) at concentrations from 0.0000015 to formed in the presence of these metabolites was determined and compared with values from untreated stromal cell cultures. (cdc.gov)
  • Ldlr(-/-) mice transplanted with EP4(+/+) or EP4(-/-) bone marrow consumed a high-fat diet for 5 or 10 weeks. (nih.gov)
  • Despite these changes, mice deficient in EP4 in bone marrow-derived cells at an advanced stage had similar lesion size (in both aorta and aortic root) as mice with EP4. (nih.gov)
  • The findings showed that in mice with non-grafted wound healing, very few bone marrow cells travelled to the wound to repair it and they did not make a major contribution to epidermal repair. (thaindian.com)
  • But in mice where a skin graft was used, a significantly higher number of specific bone marrow-derived cells travelled to the skin graft to heal the area more quickly and build new skin directly from the bone marrow cells. (thaindian.com)
  • Mice with skin grafts express high levels of HMGB1 in their blood that can drive the bone marrow repair process. (thaindian.com)
  • A UC Davis preclinical study in mice showed that when CD34+ cells were in injected into mice with retinal damage, they migrated to the affected tissue. (blindness.org)
  • In this study, we investigated the genotoxic potential of 1,1-dichloroethane in the bone marrow cells obtained from Swiss-Webster mice, using chromosomal aberrations (CA), mitotic index (MI), and micronuclei (MN) formation as toxicological endpoints. (mdpi.com)
  • 1,1-dichloroethane exposures significantly increased the number of chromosomal aberrations and the frequency of micronucleated cells in the bone marrow cells of Swiss-Webster mice. (mdpi.com)
  • Our results indicate that 1,1-dichloroethane has a genotoxic potential as measured by the bone marrow CA and MN tests in Swiss- Webster mice. (mdpi.com)
  • Previously unrecognized stem cells found in the bone marrow and blood of mice can "restock" a depleted ovary with new egg cells within weeks, according to new research published in this week's issue of the journal Cell. (bio-medicine.org)
  • This finding provides direct evidence to overturn a long-held dogma in reproductive biology, that female mice generate egg cells only during fetal development and thus are born with a finite stock of eggs that declines throughout life. (bio-medicine.org)
  • After generation of experimental endometriosis by ectopic endometrial implantation in the peritoneal cavity, bone marrow from LacZ transgenic mice was used for transplantation. (wiley.com)
  • LacZ expressing cells were found in the wild-type ectopic endometrium implanted in the peritoneal cavity of hysterectomized LacZ transgenic mice. (wiley.com)
  • Recent studies in normal mice have suggested that transplanted bone marrow cells can transdifferentiate into pancreatic β-cells at relatively high efficiency. (diabetesjournals.org)
  • Herein, adopting the same and alternative approaches to deliver and fate map-transplanted bone marrow cells in the pancreas of normal as well as diabetic mice, we further investigated the potential of bone marrow transplantation as an alternative approach for β-cell replacement. (diabetesjournals.org)
  • In contrast to previous studies, transplanted bone marrow cells expressing green fluorescence protein (GFP) under the control of the mouse insulin promoter failed to express GFP in the pancreas of normal as well as diabetic mice. (diabetesjournals.org)
  • Although bone marrow cells expressing GFP under the ubiquitously expressed β-actin promoter efficiently engrafted the pancreas of normal and hyperglycemic mice, virtually all expressed CD45 and Mac-1/Gr-1, demonstrating that they adopt a hematopoietic rather than β-cell fate, a finding further substantiated by the complete absence of GFP + cells expressing insulin and the β-cell transcription factors pancreatic duodenal homeobox factor-1 and homeodomain protein. (diabetesjournals.org)
  • 25 ) suggested that as much as 1.7-3% of the insulin-expressing cells in the islets of transplanted mice were derived from reconstituting bone marrow cells. (diabetesjournals.org)
  • Here, we have adopted different experimental strategies to follow the fate of transplanted bone marrow cells in the pancreas of normal as well as hyperglycemic mice. (diabetesjournals.org)
  • The interaction regulates the interplay between HSCs and BM stromal cells as illustrated by the decreased pool of quiescent HSCs observed in jam-b deficient mice. (bloodjournal.org)
  • 6 , 7 In mice, JAM-B expression is restricted to endothelial cells whereas JAM-C is expressed by various cell types including endothelial, 1 , 2 fibroblastic, 8 and smooth muscle cells. (bloodjournal.org)
  • After 14 weeks, the mice treated with the stem cells had a significantly reduced number of lesions in the aorta, despite no differences in cholesterol levels," Rauscher said. (brightsurf.com)
  • Transformed marrow cells injected into irradiated and methotrexate-treated recipient mice gave rise to proliferating cells which in some cases dominated the marrow population and which contained HSVtk gene sequences. (sciencemag.org)
  • To overcome this problem in mice, mAbs recognizing IPC-specific cell surface molecules have been generated. (psu.edu)
  • Using ovariectomised mice, the animal model for post-menopausal osteoporosis, the project investigated the role of Dendritic cells (DCs), the most potent antigen presenting cells, and their role in T cell activation after estrogen deficiency. (europa.eu)
  • therefore, aim of this project was to understand what was the cause of DCs activation in the bone marrow after ovx, what pathways were implicated in the downstream activation of T cells and increased TNF production and whether mice findings could be translated to human osteoporotic patients. (europa.eu)
  • Peripheral-blood stem cells versus bone marrow from unrelated donors. (nih.gov)
  • We conducted a phase 3, multicenter, randomized trial of transplantation of peripheral-blood stem cells versus bone marrow from unrelated donors to compare 2-year survival probabilities with the use of an intention-to-treat analysis. (nih.gov)
  • We did not detect significant survival differences between peripheral-blood stem-cell and bone marrow transplantation from unrelated donors. (nih.gov)
  • However, an expansion of healthy HSCs in the lab would mean that fewer stem cells need to be retrieved from donors. (medicalnewstoday.com)
  • Similarly, adhesion was unaffected by incubation of bone marrow cells in dibutyryl cyclic AMP, nor by inhibitors or donors of nitric oxide. (bl.uk)
  • A buccal swab can be used for human leukocyte antigen (HLA) typing of bone marrow donors. (medscape.com)
  • Buccal swabs are typically obtained at the time of bone marrow registry enrollment for all potential donors. (medscape.com)
  • The researchers are now investigating which types of bone-marrow cell best help repair the heart. (technologyreview.com)
  • Stem cells from adult bone marrow normally generate bone, muscle, cartilage and fat cellsa limited set compared with embryonic stem cells, which can spawn the full spectrum of adult tissues. (scientificamerican.com)
  • Dendritic cell isolation from mouse body tissues can be difficult and the number of cells isolated small. (rti.org)
  • The prior art describes the development, from stem cell to differentiated cells, of various tissues throughout the body. (google.com)
  • Stem cells are special regenerative cells with which the body replaces or repairs its own tissues. (nytimes.com)
  • The native skin cells produced only collagen type I. Researchers do not know why bone marrow would produce collagen III, which is typically found in connective tissues such as skin. (innovations-report.com)
  • The recognition that stem cells, particularly those from the bone marrow, have the capacity to colonize different tissues, proliferate, and transdifferentiate into cell lineages of the host organ ( 8 , 9 ), prompted us in an earlier study ( 10 ) to inject Lin − c- kit POS bone marrow cells (BMC) in the contracting myocardium bordering acute infarcts. (pnas.org)
  • Two main determinants seem to be critical for colonization and transdifferentiation of BMC into a variety of tissues: recent damage and a high number of circulating stem cells ( 8 , 9 , 11 , 12 ). (pnas.org)
  • The ECM serves a unique role by constructing a "scaffold" for cells, thereby maintaining the structural integrity of many tissues. (emaxhealth.com)
  • We also discuss the mechanisms by which BMSCs control neuroinflammation and chronic pain and how these cells specifically migrate to damaged tissues. (rutgers.edu)
  • But a new study published in Cell Transplantation found that, when injected directly into damaged heart tissue, cells originating from the tissues surrounding the blood vessels of the human umbilical cord are superior to those of marrow. (healthcanal.com)
  • The growing evidence suggesting that they can recirculate and migrate to injured tissues to replace lost parenchymal cells, together with the possibility of rapidly expanding them ex vivo without loss of differentiation potential, 1 - 4 make them of substantial clinical interest. (bloodjournal.org)
  • Photobiomodulation effects of Low-level light irradiation (LLLI) on regeneration have been reported in skin, nerve, and skeletal muscle tissues and bone. (scirp.org)
  • The study, released in Cell Transplantation this month, demonstrates that the cells originating from the tissues surrounding the blood vessels of the human umbilical cord, also known as "Wharton's Jelly," outperformed the current gold standard for stem cell therapies for repairing damage to heart muscles, after an induced heart attack when injected directly into the affected area. (bio-medicine.org)
  • Identification and functional characterization of these cells have been difficult because of their small numbers in blood and tissues and their complex cell surface phenotype. (psu.edu)
  • Although IPC have significant effects on other cells, their numbers in blood and tissues are very small. (psu.edu)
  • While much is understood about dendritic cells and their role in the immune system, the study of these cells is critical to gain a more complete understanding of their function. (rti.org)
  • This protocol describes the growth of large number of dendritic cells from the culture of mouse bone marrow cells. (rti.org)
  • A resident population of dendritic cells (DCs) has been identified in murine bone marrow, but its contribution to the regulation of hematopoiesis and establishment of the stem cell niche is largely unknown. (jci.org)
  • T ype I IFN-producing cells (IPC),3 also called plasmacy-toid dendritic cells (DC), are early responders to viralinfections (1, 2). (psu.edu)
  • The project OSTEODEN investigated the role of bone marrow dendritic cells in the development of post-menopausal osteoporosis, aiming to further elucidate the involvement of the immune system in this pathology and to develop new potential therapeutic approaches. (europa.eu)
  • These multipotent cells can be differentiated in vitro and in vivo into various cell types of mesenchymal origin, such as osteoblasts, adipocytes, and chondrocytes [ 3 , 4 ]. (hindawi.com)
  • Rabbit anti-thymocyte globulin (ATG (Thymoglobulin)) kills T cells in vitro and probably also in vivo as it prevents graft-vs-host disease (GvHD) in patients. (nature.com)
  • Recently we demonstrated that ATG at a clinically relevant concentration (10-50 mg/L) kills in vitro not only T cells but also leukemic blasts. (nature.com)
  • Treatment of endothelial cells in vitro with CXCL1 induced increased vascular permeability and HSPC transmigration. (jci.org)
  • In vitro effects of benzene metabolites on mouse bone marrow stromal cells. (cdc.gov)
  • The effects of benzene (71432) metabolites on bone marrow stromal cells were investigated in-vitro. (cdc.gov)
  • The authors conclude that benzene metabolites are toxic to mouse bone marrow stromal cells in-vitro. (cdc.gov)
  • DNA containing the herpes simplex virus thymidine kinase (HSVtk) gene was used to transform wild-type tk+ mouse L cells to a tk++ status in vitro using methotrexate as a selective agent. (sciencemag.org)
  • HSVtk DNA was also used to transform mouse bone marrow cells in vitro. (sciencemag.org)
  • For this purpose, an in vitro murine model was used in which T-cell responses were generated against the male HY minor histocompatibility antigen. (bloodjournal.org)
  • Defining the cellular composition of these niches, their location within the tissue and their secretion of signalling factors is indispensable for understanding how the bone marrow ensures a balanced blood cell production. (nature.com)
  • Bone marrow is the soft, fatty tissue inside your bones. (medlineplus.gov)
  • This study shows that bone marrow stem cells, in addition to cells from the surrounding tissue, may actually contribute to the healing process. (innovations-report.com)
  • Two critical determinants seem to be required for the transdifferentiation of primitive BMC: tissue damage and a high level of pluripotent cells. (pnas.org)
  • BERKELEY - When stretched, a type of adult stem cell taken from bone marrow can be nudged towards becoming the type of tissue found in blood vessels, according to a new study by bioengineers at the University of California, Berkeley. (berkeley.edu)
  • It was a cellular workout routine that helped point the stem cell in the direction of becoming the smooth muscle tissue of vascular walls. (berkeley.edu)
  • Mesenchymal stem cells have the ability to turn into different types of connective tissue including bone, cartilage and muscle. (berkeley.edu)
  • Embryonic stem cells have the advantage of being able to turn into any kind of body tissue and of being easier to work with in the lab, though that flexibility comes with controversy and ethical questions not found in research on adult stem cells. (berkeley.edu)
  • Bone marrow biopsy and aspiration is the removal of some bone marrow tissue for diagnosis and management of cancers of blood cells and multiple myeloma. (medindia.net)
  • Stem cells perform the vital function of supporting surrounding tissue by providing new daughter somatic cells to make up losses and take their place to maintain tissue function. (medworm.com)
  • Lingering senescent cells secrete a potent mix of signals that rouse the immune system into a state of chronic inflammation, and degrade tissue function and structure. (medworm.com)
  • Washington, April 05 (ANI): Scientists have identified specific bone marrow cells that can transform into skin cells to repair damaged skin tissue. (thaindian.com)
  • The key achievement has been to find out which bone marrow cells can transform into skin cells and repair and maintain the skin as healthy tissue, and to learn how this process happens," said Professor John McGrath, Head of the Genetic Skin Disease Group at King's, who has spent several months working on the project in Osaka. (thaindian.com)
  • Led by Dr. Susanna Park , the investigative team believes the cells may promote the healing and survival of affected retinal tissue. (blindness.org)
  • That's because the damaged tissue releases factors which attract the cells. (blindness.org)
  • In the late 1940s, University of Chicago researcher Dr. Leon Jacobson discovered that he could save a mouse, whose bone marrow and spleen had been destroyed with radiation, by transplanting healthy spleen tissue from another mouse. (uchospitals.edu)
  • The donated tissue repopulated the marrow and restored production of the blood cells. (uchospitals.edu)
  • Most stem cell therapies use cells harvested from bone marrow to stimulate tissue repair and control inflammation. (healthcanal.com)
  • The results could represent a paradigm shift for stem cell research, researchers said, because the umbilical cord is a tissue typically discarded after birth. (healthcanal.com)
  • He is also interested in conducting further research with the umbilical cord cells to overcome the damaging effects of chemotherapy on heart tissue, an agonizing problem for some patients who may be cured of their cancer only to confront heart failure as a result of treatment. (healthcanal.com)
  • There are currently more than 250 clinical trials being conducted worldwide using stem cells to investigate the treatment of a variety of diseases, including a serious complication of bone marrow transplantation called graft-versus-host disease, autoimmune disorders, neurological diseases and tissue injury arising from lung and liver disease. (healthcanal.com)
  • Tissue Regeneration Therapeutics (TRT), the Toronto-based company that created the technology platform for manufacturing the cells, provides cells to leading researchers around the world free of charge. (healthcanal.com)
  • Stem cells are special cells produced by bone marrow (a spongy tissue found in the centre of some bones) that can turn into different types of blood cells. (axappphealthcare.co.uk)
  • The cells were placed into T-25 tissue (Greiner, Frickenhausen, Germany) culture flasks at 37˚C in a 5% CO 2 atmosphere. (scirp.org)
  • At present, mesenchymal cells, known to release a series of factors that stimulate tissue repair, and control inflammation, are most commonly harvested from bone marrow. (bio-medicine.org)
  • The HUCPVC cell therapy was twice as effective at repairing damage to heart tissue than no treatment. (bio-medicine.org)
  • Apart from heart disease, clinical trials with mesenchymal cells are conducted around the world to investigate the treatment of a variety of diseases, including a serious complication of bone marrow transplantation called graft-versus-host disease, autoimmune disorders, neurological diseases and tissue injury arising from lung and liver disease. (bio-medicine.org)
  • The data help in understanding the role of the external mechanical determinants in stem cell differentiation, and can also be useful in translational approach in functional tissue engineering. (wiley.com)
  • BMSCs have been widely used for tissue engineering, especially for bone regeneration. (umich.edu)
  • Distinct subtypes of endothelial cells, perivascular cells and other stromal and haematopoietic cells have been characterised as components of these HSC niches and represent key contributors to niche function 1 . (nature.com)
  • Ablation of cDCs was associated with an expansion of bone marrow endothelial cells and increased vascular permeability. (jci.org)
  • CXCR2 expression in sinusoidal endothelial cells and the expression of 2 CXCR2 ligands, CXCL1 and CXCL2, in the bone marrow were markedly increased following cDC ablation. (jci.org)
  • Some turned into endothelial cells lining the artery, while others turned into the smooth muscle cells beneath the endothelium that help move blood through the arteries. (brightsurf.com)
  • Mouse bone marrow contains spontaneous rosette-forming cells (RFC) which include more than 70% T-cell precursors, as assessed by their transformation into theta-positive cells after incubation with thymic hormone. (biomedsearch.com)
  • Such spontaneous RFC, examined in C57B1/6 mouse bone marrow by electron and scanning electron microscopy, have consistently been shown to be small, inactive mouse lymphocytes when macrophages have been eliminated by cell preincubation. (biomedsearch.com)
  • With the help of several genetic markers that are found in germ cells, the master cells that eventually give rise to the egg and sperm, the team shows evidence for the existence of germ cell progenitors, putative stem cells, in mouse bone marrow and blood. (bio-medicine.org)
  • It was also found that indomethacin reversed the PTH mediated reduction of CFU-F that could be extracted from mouse bone marrow. (bl.uk)
  • Also, other investigators have shown that it is possible to directly insert HOXB4 protein into extracted bone marrow stem cells. (medicalnewstoday.com)
  • Increasing tPa activity in astrocytes induced by multipotent mesenchymal stromal cells facilitate neurite outgrowth after stroke in the mouse," PLoS ONE , vol. 5, no. 2, Article ID e9027, 2010. (hindawi.com)
  • CD105 is expressed on mesenchymal stromal cells. (stemcell.com)
  • Low/Negative Expression of PDGFR-α Identifies the Candidate Primary Mesenchymal Stromal Cells in Adult Human Bone Marrow. (lu.se)
  • NewYork-Presbyterian offers bone marrow and stem cell transplantation for people with hematological malignancies (e.g., leukemia, lymphoma, multiple myeloma, myelodysplastic syndromes (MDS)) for myeloproliferative disorders such as myelofibrosis, for AL-amyloidosis, and for non-malignant blood disorders such as severe aplastic anemia and sickle cell anemia (SCA). (nyp.org)
  • Randomized trials have shown that the transplantation of filgrastim-mobilized peripheral-blood stem cells from HLA-identical siblings accelerates engraftment but increases the risks of acute and chronic graft-versus-host disease (GVHD), as compared with the transplantation of bone marrow. (nih.gov)
  • Some studies have also shown that peripheral-blood stem cells are associated with a decreased rate of relapse and improved survival among recipients with high-risk leukemia. (nih.gov)
  • Exploratory analyses of secondary end points indicated that peripheral-blood stem cells may reduce the risk of graft failure, whereas bone marrow may reduce the risk of chronic GVHD. (nih.gov)
  • Peripheral blood stem cells are harvested from donated blood. (cookchildrens.org)
  • Since it is a simple procedure to collect stem cells from bone marrow, we hope that our research paves the way forward into clinical trials. (redorbit.com)
  • The procedure builds on promising work in animals showing that stem cells from the bone marrow can be used to repair the heart. (nytimes.com)
  • Unlike the German procedure, which required a painful extraction of cells from the bone marrow, he extracted white blood cells from the blood with a technique that captures bone marrow stem cells that circulate in the bloodstream. (nytimes.com)
  • The procedure consisted of removing 300 milliliters of bone marrow from her hip and giving it back to her intravenously. (medicalnewstoday.com)
  • Bone marrow transplantation (or stem cell transplantation) is a well-established procedure for patients with relapsed or high-risk cancers of the blood, such as leukaemia , lymphoma and myeloma . (guysandstthomas.nhs.uk)
  • Bone marrow stem cells are collected from the donor's hip bone through a surgical procedure. (cookchildrens.org)
  • About 23% of the time, doctors request marrow - a surgical, outpatient procedure that takes place at a hospital. (ne.gov)
  • When blood stem cells are collected from the bloodstream, the procedure is called a peripheral blood stem cell collection or harvest. (bmtinfonet.org)
  • Cultured lung fibroblasts expressed the chemokine receptors CXCR4 and CCR7 and responded chemotactically to their cognate ligands, stromal cell-derived factor-1α and secondary lymphoid chemokine, respectively. (jci.org)
  • Combined with the recent evidence of BM stem cell plasticity cited above, this provides a compelling argument for re-examination of the origin of the fibroblasts in pulmonary fibrosis, especially in terms of their possible extrapulmonary origin. (jci.org)
  • Our study shows that the recruitment of bone marrow-derived fibroblasts is important for promoting tumor growth, likely by enhancing blood vessel formation," Neta Erez, researcher at Sackler School of Medicine, Tel Aviv University, said in a press release. (upi.com)
  • Breast cancer tumors with these bone marrow-associated fibroblasts can therefore grow more blood vessels that allow them to develop more quickly than tumors without fibroblast interactions. (upi.com)
  • Erez and her colleagues observed that human breast cancer tumors also have fibroblasts without PDGFRα, meaning human tumors may also pull in bone marrow cells. (upi.com)
  • Understanding the function of these cancer-associated fibroblasts could form the basis of developing novel therapeutic manipulations that co-target bone marrow-derived fibroblasts as well as the cancer cells themselves," Erez said. (upi.com)
  • These bone marrow-derived fibroblasts are different from other cancer-associated fibroblasts. (medindia.net)
  • But bone marrow-derived fibroblasts are particularly effective at stimulating the formation of new blood vessels because they produce large amounts of a protein called clusterin. (medindia.net)
  • Tumors containing bone marrow-derived fibroblasts were therefore more vascularized and grew faster than tumors that only contained breast-derived fibroblasts. (medindia.net)
  • Erez and colleagues found that human breast cancers also contain fibroblasts lacking PDGFRα, suggesting that human tumors may also recruit bone marrow-derived cells. (medindia.net)
  • Moreover, tumors containing lower levels of PDGFRα tended to be more deadly, suggesting that the recruitment of bone marrow-derived fibroblasts is a crucial step in breast cancer progression. (medindia.net)
  • Our study shows that the recruitment of bone marrow-derived fibroblasts is important for promoting tumor growth, likely by enhancing blood vessel formation," Erez says. (medindia.net)
  • A mouse breast tumor contains bone marrow-derived fibroblasts (red) as well as other cancer-associated fibroblasts (green). (eurekalert.org)
  • The study, which will be published November 23 in the Journal of Experimental Medicine , reveals that the recruitment of bone marrow-derived fibroblasts lowers the odds of surviving breast cancer, but suggests that targeting these cells could be an effective way of treating the disease. (eurekalert.org)
  • Myelofibrosis causes an abnormal increase in the cells that produce collagen fibers called fibroblasts. (news-medical.net)
  • The cells known as fibroblasts are thought to be these young osteoblasts. (news-medical.net)
  • Recombinant fragment, corresponding to a region within amino acids 1-146 of Human Bone marrow stromal cell antigen 1. (abcam.com)
  • If so, it would not be necessary to use the ethically more problematic embryonic stem cells. (eurekalert.org)
  • Moreover, bone marrow cells may also represent an attractive source for cell replacement therapy of type 1 and type 2 diabetes, not least in view of the limited supply of human islets and ethical issues surrounding embryonic stem cell research ( 28 - 31 ). (diabetesjournals.org)
  • These stromal cells organise into multicellular niches that produce cytokines, chemokines, adhesion molecules and other factors to regulate the self-renewal and differentiation of blood cells 1 . (nature.com)
  • Synergistic effects of CoCl 2 and ROCK inhibition on mesenchymal stem cell differentiation into neuron-like cells," Journal of Cell Science , vol. 119, no. 13, pp. 2667-2678, 2006. (hindawi.com)
  • Stimulated astrocytes release high-mobility group 1 protein, an inducer of LAN-5 neuroblastoma cell differentiation," Neuroscience , vol. 82, no. 4, pp. 1021-1028, 1997. (hindawi.com)
  • The findings, published today (Monday, Oct. 23) in the online early edition of the Proceedings of the National Academy of Sciences , highlight the importance of mechanical forces in stem cell differentiation. (berkeley.edu)
  • Experiments in stem cell differentiation, however, have traditionally relied upon chemical signals to prompt this transformation into the desired cell type. (berkeley.edu)
  • We are now extending this concept to the cellular level by showing that mechanical stimulation can impact stem cell differentiation. (berkeley.edu)
  • This new study is the first to look at the effects of such uniaxial strain on stem cell differentiation. (berkeley.edu)
  • Ectopic differentiation of stem cells may be a novel mechanism of disease. (wiley.com)
  • We have identified a subpopulation of stem cells within adult human BM, isolated at the single-cell level, that self-renew without loss of multipotency for more than 140 population doublings and exhibit the capacity for differentiation into cells of all 3 germ layers. (jci.org)
  • Intramyocardial transplantation of hBMSCs after myocardial infarction resulted in robust engraftment of transplanted cells, which exhibited colocalization with markers of cardiomyocyte (CMC), EC, and smooth muscle cell (SMC) identity, consistent with differentiation of hBMSCs into multiple lineages in vivo. (jci.org)
  • Chemical induction of osteoblastic differentiation from BM stromal cells also induced an increase in FL production. (irsn.fr)
  • The effects of aging on stem cell properties and indicators of stem cell fitness such as proliferation, differentiation, circadian rhythms, stress response proteins, miRNA expression, and global histone modifications in rBMSCs were analyzed. (biomedsearch.com)
  • We found that osteogenic differentiation can be regulated by the rigidity of the substrate, which may depend on the commitment in multi- or uni-potent targeting cells. (wiley.com)
  • Through secretion of type I IFN, IPC direct both the innate and adaptive immune re-sponse by promoting NK cell and CD8 T cell cytotoxicity, en-hancing DC maturation, presenting Ag to T cells and inducing their Th1 polarization, and inducing B cell differentiation into Ab-secreting plasma cells (1, 2). (psu.edu)
  • Although the notion that ROS production was implicated in osteoclasts formation was not new, we first made a link between oxidative stress, T cell activation, TNF production and osteoclasts differentiation, thereby contributing to reconcile two different major hypotheses, i.e. that ovx-induced bone loss was either caused by increased oxidative stress or by increased TNF production by T cells. (europa.eu)
  • Therefore, we came to the conclusion that estrogen deficiency sent a stress signal in bone through ROS, resulting in DC and T cell activation and ultimately leading to increase TNF production by T cells and osteoclast differentiation. (europa.eu)
  • The relevance of this pathway was conclusively demonstrated by a pharmacological approach, where the immunosuppressant CTLA4-Ig, a recently developed drug that inhibited the CD80 of T cell activation, was employed and proved to be effective in preventing ovx-induced T cell activation, T cell TNF production, increased osteoclasts differentiation and bone loss. (europa.eu)
  • Vitamin D is a hormone that regulates calcium, and the team had already shown that vitamin D receptors control the location of hematopoietic cells in the bone marrow. (news-medical.net)
  • Here, we show that murine bone marrow DCs are perivascular and have a type 2 conventional DC (cDC2) immunophenotype. (jci.org)
  • To test the effects of PTH in vivo, the number of CFU-F that could be extracted from murine bone marrow after administration of an anabolic dose of PTH were measured. (bl.uk)
  • Collectively, these data suggest that bone marrow DCs play an important role in regulating HSPC trafficking, in part, through regulation of sinusoidal CXCR2 signaling and vascular permeability. (jci.org)
  • Recent studies suggest that bone marrow stem cells or bone marrow stromal cells (BMSCs) produce powerful analgesic effects in animal models of inflammatory pain, neuropathic pain, and cancer pain. (rutgers.edu)
  • Previously, researchers had not known that bone marrow contributed to the development of new skin in wounds. (innovations-report.com)
  • We've known that bone marrow cells are involved in wound healing and inflammation - now we have data that shows bone marrow cells are involved in normal skin maintenance, in maintaining the matrix environment and integrity of the skin. (innovations-report.com)
  • It was already known that bone marrow may play a role in skin wound healing, but until now it was not known which specific bone marrow cells this involves, how the process is triggered, and how the key cells are recruited to the affected skin area. (thaindian.com)
  • New research suggests that breast cancers can increase their growth by recruiting stromal cells that form in bone marrow. (upi.com)
  • Breast tumors boost their growth by recruiting stromal cells from the bone marrow. (medindia.net)
  • Researchers in Israel have discovered that breast tumors can boost their growth by recruiting stromal cells originally formed in the bone marrow. (eurekalert.org)
  • S. Law and S. Chaudhuri, "Mesenchymal stem cell and regenerative medicine: regeneration versus immunomodulatory challenges," American Journal of Stem Cells , vol. 2, no. 1, pp. 22-38, 2013. (hindawi.com)
  • Despite the demonstration that a subpopulation of cardiac muscle cells is able to replicate ( 1 ), and new vessels are formed ( 2 ), this regeneration is restricted to the viable myocardium. (pnas.org)
  • Thus, the capacity of transplanted bone marrow cells to contribute to β-cell regeneration remains controversial. (diabetesjournals.org)
  • Conditioning protocols were tested for their efficacy in increasng the incidence of engraftment of histoincompatible dog bone marrow cells. (osti.gov)
  • Pilot studies of autologous stem cell transplantation using peripheral blood have demonstrated rapid engraftment with this technique. (medscape.com)
  • Osteoblasts, the bone-forming cells, derive from multipotential bone marrow stromal precursors called colony-forming units-fibroblastic (CFU-F). CFU-F rapidly adhere to plastic upon culture ex vivo, adhesion of such stromal precursors to bone in vivo is likely to be an early event in the anabolic response to bone stimulatory factors. (bl.uk)
  • One is indoleamine 2,3-dioxygenase (IDO), which, by catalyzing the conversion from tryptophan to kynurenine, exerts a major immunosuppressive effect on T-cell responses to autoantigens and fetal alloantigens in vivo. (bloodjournal.org)
  • However, for clinical application currently, large quantities of hBMSCs are usually required for transplantation which is typically produced by serial passages of the cells ex vivo . (umich.edu)
  • Peripheral blood stem cell collection. (medlineplus.gov)
  • Patients were randomly assigned in a 1:1 ratio to peripheral-blood stem-cell or bone marrow transplantation, stratified according to transplantation center and disease risk. (nih.gov)
  • A research team led by Weill Cornell Medical College investigators may have solved a major issue of expanding adult hematopoietic stem cells (HSCs) outside the human body for clinical use in bone marrow transplantation - a critical step towards producing a large supply of blood stem cells needed to restore a healthy blood system. (medicalnewstoday.com)
  • Additional rounds of bone marrow harvest and clinical applications to mobilize blood stem cells are often required. (medicalnewstoday.com)
  • It also suggests that adult blood stem cells could be frozen and banked for future expansion and use - which is not currently possible. (medicalnewstoday.com)
  • Our work demonstrates that we can overcome a major technical hurdle in the expansion of adult blood stem cells, making it possible, for the first time, to produce them on an industrial scale,' says the study's senior investigator, Dr. Pengbo Zhou, professor of pathology and laboratory medicine at Weill Cornell. (medicalnewstoday.com)
  • Not only are a person's own blood stem cells the best therapy for many blood cancers, but they may also be useful for other purposes, such as to slow aging. (medicalnewstoday.com)
  • At any rate, blood stem cells do not convert to heart muscle cells in a damaged heart, which was previously hoped. (eurekalert.org)
  • It was about transplanting blood stem cells to create new heart muscle cells to repair a heart after a heart attack. (eurekalert.org)
  • In other words, fusions may explain the first promising studies: the scientists believed they were looking at cells produced by maturation of blood stem cells, whereas in actual fact they were seeing a tiny number of fused cells. (eurekalert.org)
  • Cord blood stem cells are collected from the umbilical cord at time of delivery and are then frozen for later use. (cookchildrens.org)
  • Follow a former NCI employee as she takes you through her blood stem cell donation experience at the NIH Clinical Center in Bethesda, MD. (cancer.gov)
  • The largest concentration of blood stem cells is in your bone marrow. (bmtinfonet.org)
  • However, the blood stem cells can be moved or "mobilized" out of the bone marrow into the bloodstream (peripheral blood) where they can be easily collected. (bmtinfonet.org)
  • Peripheral blood stem cell collections are done in an outpatient clinic. (bmtinfonet.org)
  • The amount of bone marrow harvested depends on the size of the patient and the concentration of blood stem cells in your marrow. (bmtinfonet.org)
  • By touching an electro-sensitive tip around the chamber's surface, the researchers were able to locate areas of low electrical activity, where diminished blood supply had caused cells to die. (technologyreview.com)
  • In 25 of the patients, the researchers injected around 100,000 cells into angina-affected areas on the ventricular surface, using a modified form of the same catheter. (technologyreview.com)
  • Earlier trials in which researchers sought to treat heart-attack victims with their own bone-marrow cells produced mixed results. (technologyreview.com)
  • After experimenting with several different cocktails, the researchers hit on a mix that transformed 100 percent of cultured stem cells into those that looked like neurons in under an hour. (scientificamerican.com)
  • STOCKHOLM (Reuters) - Patients with chronic heart failure given injections of their own bone marrow stem cells have better heart function and live longer, German researchers said Sunday. (news24.com)
  • One of the German studies, published last September by researchers at the Heinrich-Heine University in Düsseldorf, reported that 10 patients had shown a diminished area of heart muscle damage three months or more after treatment with cells from their own bone marrow. (nytimes.com)
  • Researchers found green cells throughout the body, but observed that the highest concentration of bone marrow cells was in normal skin. (innovations-report.com)
  • Researchers found that even after six weeks, long after the infection-fighting role seems to be over, the bone marrow-derived cells cluster within the healing area of a wound. (innovations-report.com)
  • The researchers ran these skin cells through a flow cytometer to separate them into green and non-green fractions and found only the green cells in the skin produced collagen type III, which is one of the two most abundant collagens in skin. (innovations-report.com)
  • Researchers placed mesenchymal stem cells, extracted from bone marrow, onto a silicone membrane that was stretched longitudinally once every second. (berkeley.edu)
  • In an effort to better understand the factors that affect the eventual fate of mesenchymal stem cells, the researchers designed the experiment to simulate the physical forces a cell would encounter if it were to become a blood vessel. (berkeley.edu)
  • The researchers placed a single layer of mesenchymal stem cells onto a membrane with microgrooves to resemble the patterns formed in blood vessels by collagen fibers. (berkeley.edu)
  • The researchers also looked at the effects of stretching stem cells on a smooth membrane with no microgrooves. (berkeley.edu)
  • After two days of this cellular exercise regimen, the researchers found a significant increase in the expression of a group of genes that control tensile strength compared with cells that were not stretched. (berkeley.edu)
  • While emphasizing that much more work should be done to confirm these findings, researchers believe that bone marrow cells may one day be used to treat neurological diseases such as Parkinson's disease. (medindia.net)
  • Human bone marrow has been used to create early-stage sperm cells for the first time, a scientific step forward that will help researchers understand more about how sperm cells are created. (emaxhealth.com)
  • April 11, 2013 - Researchers from the University of California, Davis (UC Davis), have launched a Phase I clinical trial of CD34+ bone marrow stem cells (BMSC) for people with retinal conditions that cause vision loss from ischemia, or loss of blood flow, and cell degeneration. (blindness.org)
  • In addition, the researchers also found these markers in human bone marrow and blood. (bio-medicine.org)
  • Finally, the researchers would like to find out whether these stem cells could be coaxed to produce eggs in the laboratory, potentially providing a new source of eggs for therapeutic cloning. (bio-medicine.org)
  • The umbilical cord cells were 120% more effective in improving heart function than bone marrow cells - a statistically significant difference, researchers said. (healthcanal.com)
  • CHICAGO -- Duke University Medical Center researchers have shown that an age-related loss of specific stem cells that continually repair damage to blood vessels is critical to determining the onset and progression of atherosclerosis. (brightsurf.com)
  • TORONTO, Ontario (13 November, 2012) - A study published this month by researchers at the University of Toronto and Toronto's Princess Margaret Hospital has shown that cells derived from the umbilical cord, "Human Umbilical Cord PeriVascular Cells" (HUCPVCs), are more effective in restoring heart function after an acute myocardial infarction (in common parlance, a heart attack) in a pre-clinical model than a similar cell population derived from bone marrow. (bio-medicine.org)
  • Researchers also found that certain types of the stem cells were associated with the largest improvement and warrant further study. (healthcanal.com)
  • The researchers looked at the makeup of these patients' stem cells from a supply stored at a biorepository established by the CCTRN. (healthcanal.com)
  • According to a study by Japanese researchers, the SMILE combination chemotherapy protocol is effective against extranodal natural killer/T-cell. (lymphomainfo.net)
  • New findings suggest that a biochemical cocktail can coax adult bone marrow stem cells to become neurons, according to a report presented yesterday at the annual meeting of the Society for Neuroscience in San Diego. (scientificamerican.com)
  • Functional neural stem cells derived from adult bone marrow. (nih.gov)
  • Pluripotent hematopoietic cells from adult bone marrow may give rise not only to neurons, oligodendrocytes and astrocytes after transplantation into newborn brains, but also to neural stem cells (NSC). (nih.gov)
  • 22 ] placed fibrin glue and whole bone marrow aspirate into defects in the avascular zone of the meniscus. (hindawi.com)
  • Anumber of recent studies ( 1 - 8 ) have suggested that bone marrow transplantation may give rise to nonhematopoietic cell lineages in multiple organs by a process termed transdifferentiation. (diabetesjournals.org)
  • It can be used to treat conditions affecting the blood cells, such as leukaemia and lymphoma. (axappphealthcare.co.uk)
  • Functional recovery after hematic administration of allogenic mesenchymal stem cells in acute ischemic stroke in rats," Neuroscience , vol. 175, pp. 394-405, 2011. (hindawi.com)
  • ATG-induced death of acute myeloid leukemia (AML) cells from patients newly diagnosed with AML was measured among blasts as well as LSCs. (nature.com)
  • Their stem cells are more likely to help patients than stem cells from older people. (medlineplus.gov)
  • Previous studies have suggested that cell therapy can be effective in patients who have suffered a heart attack. (news24.com)
  • The study included 391 patients, of whom 191 agreed to have the bone marrow stem cell treatment and 200 did not. (news24.com)
  • The aim of the study is to determine the safety and tolerability of an autologous CD34+ subset bone marrow stem cell infusion into the middle cerebral artery in patients who have suffered acute total or partial anterior circulation syndrome (TACS/PACS). (clinicaltrials.gov)
  • In this study we will evaluate safety and feasibility of autologous bone marrow mono nuclear (BM-MNC) and enriched CD133+ hematopoietic stem cell transplantation through the portal vein in patients with decompensate cirrhosis. (clinicaltrials.gov)
  • After cell therapy, patients are followed up every week for 4 weeks, and laboratory data are analyzed for 24 weeks. (clinicaltrials.gov)
  • This two (2) day program is designed for Registered Nurses and Nurse Practitioners caring for patients undergoing Hematopoietic Stem Cell Transplantation (HSCT). (mskcc.org)
  • So far, the treatment has been well-tolerated, and we have preliminary data from the macular degeneration patients suggesting that the cells are incorporating into the retina. (blindness.org)
  • Select patients are candidates for outpatient stem cell transplantation, an approach that offers the full benefits of this life-saving treatment while allowing the patient to spend nights in the comfort of their own home. (uchospitals.edu)
  • Relapse remains the biggest challenge for cancer patients who have undergone stem cell transplantation. (uchospitals.edu)
  • However, treatments preventing their release from bone marrow may help reduce the exaggerated immune response that contributes to poor outcomes in patients with severe COVID-19. (manchester.ac.uk)
  • Keating, director of the Cell Therapy Program at the Princess Margaret Cancer Centre and University Health Network (UHN), said he hopes to begin clinical trials of the HUCPVC cells on patients within the next 12 to 18 months. (healthcanal.com)
  • We really don't have anything else to offer patients with sickle cell disease' who do not respond to hydroxyurea, the only drug useful in treating it, said the paper's senior author, Dr. John F. Tisdale of the National Heart, Lung and Blood Institute. (dailypress.com)
  • Patients receive frequent transfusions to provide competent red cells, but that leads to a toxic buildup of iron and can sensitize patients to foreign red cells. (dailypress.com)
  • Keating and his team will now complete additional pre-clinical studies, and hope to begin clinical trials of the HUCPVC cells on patients within 12-18 months. (bio-medicine.org)
  • This Phase II clinical trial, designed to test this strategy to improve cardiac function, is an extension of earlier efforts in Brazil in which a smaller number of patients received fewer stem cells. (healthcanal.com)
  • For this new network study, 92 patients received a placebo or 100 million stem cells derived from the bone marrow in their hips in a one-time injection. (healthcanal.com)
  • In some patients, particularly those who were younger or whose bone marrows were enriched in certain stem cell populations, had even greater improvements in their ejection fractions. (healthcanal.com)
  • They found these patients had more CD34+ and CD133+ type of stem cells in their mixture. (healthcanal.com)
  • It also suggests new directions for the next series of clinical trials, including the type of patients, endpoints to study and types of cells to deliver. (healthcanal.com)
  • These changes represent novel insights into the aging process and could have implications regarding the potential for autologous stem cells therapy in older patients. (biomedsearch.com)
  • The number of KSL ( D ) or CFU-C ( E ) in bone marrow, blood, and spleen are shown. (jci.org)
  • Recent evidence has implicated bone marrow-derived cells as possible endometrial progenitors. (wiley.com)
  • He said that many centers were planning clinical trials of stem cell therapy, based on recent data from animals, and that the fact that an operation had now been tried was ''not a real surprise -- it only happened because this teenager had this unfortunate accident. (nytimes.com)
  • These stem cells have been used to cure diseases since the 1960's. (godandscience.org)
  • Scientists have discovered a new way to replicate the regenerative power of stem cells in the lab, which could lead to powerful treatments for injuries and diseases. (phys.org)
  • This condition can make it hard to control other diseases, and bone hardening (osteosclerosis) also occurs. (news-medical.net)
  • Certain diseases and treatments can deplete a child's healthy stem cells. (cookchildrens.org)
  • You have mentioned diseases of the blood marrow in the column before, but what does the bone marrow actually do? (dcourier.com)
  • Diseases of the bone marrow can cause problems by making something abnormal (such as leukemia cells), but also by failing at its job and not making what it is supposed to. (dcourier.com)
  • Bone marrow diseases sometimes can be treated directly, but often treatment involves replacing blood products, and possibly using growth factors to make the bone marrow work better. (dcourier.com)
  • Now, treatment of a number of diseases is possible through stem cell transplantation. (medscape.com)
  • New research, published in BioMed Central's open access journal Stem Cell Research & Therapy, investigates the therapeutic use of human stem cells from bone marrow against acute lung injury and identifies TNF-Ã ±-induced protein 6 as a major molecular component of stem cell action. (redorbit.com)
  • The cells or blood is then tested for special proteins, called human leukocytes antigens (HLA). (medlineplus.gov)
  • Intranasal administration of human umbilical cord mesenchymal stem cells-conditioned medium enhances vascular remodeling after stroke," Brain Research , vol. 1624, pp. 489-496, 2015. (hindawi.com)
  • Ischemic rat brain extracts induce human marrow stromal cell growth factor production," Neuropathology , vol. 22, no. 4, pp. 275-279, 2002. (hindawi.com)
  • Human leukocyte antigen-G5 secretion by human mesenchymal stem cells is required to suppress T lymphocyte and natural killer function and to induce CD4+ CD25highFOXP3+ regulatory T cells," Stem Cells , vol. 26, no. 1, pp. 212-222, 2008. (hindawi.com)
  • A similar population of DCs was present in human bone marrow. (jci.org)
  • Human cells shed exosomes. (infowars.com)
  • http://neuromics.blogspot.com/2019/04/we-have-human-bone-marrow-derived-stem.htm. (medworm.com)
  • The present invention relates to proteins associated with human bone marrow cell membranes for adhering hematopoietic cells to human bone marrow cell membranes. (google.com)
  • Based on surface marker expression, these clonally expanded human BM-derived multipotent stem cells (hBMSCs) do not appear to belong to any previously described BM-derived stem cell population. (jci.org)
  • To our knowledge, this is the first demonstration that a specific population of multipotent human BM-derived stem cells can induce both therapeutic neovascularization and endogenous and exogenous cardiomyogenesis. (jci.org)
  • Human umbilical vein ECs (lane 4) and immortalized cell lines with high telomere length, provided by the Roche Diagnostics Corp. (lane 5), were used as controls. (jci.org)
  • Coloured Scanning Electron Micrograph (SEM) of human bone marrow stem cells. (sciencephoto.com)
  • Human Leukocyte Antigens (HLA) is a type of protein that is found on the outer surfaces of cells. (wdxcyber.com)
  • Purpose: To define the ability of human bone marrow (BM) stromal cells to produce Flt3 ligand (FL), and to define the effect of ionizing radiation, TNF or TGF on FL production. (irsn.fr)
  • Oswaldo Tapanes received multiple injections of human umbilical cord-derived mesenchymal stem cells and his own bone marrow-derived stem cells over the course of a month both intrathecally (into the spinal fluid) and intravenously at the Stem Cell Institute in Panama. (cellmedicine.com)
  • Human bone marrow stromal cells (hBMSCs) are adherent fibroblast-like cells found in the bone marrow. (umich.edu)
  • For gene expression comparison, total RNA was extracted from hBMSCs using RNeasy Kit (Qiagen) and gene expression was examined with human Mesenchymal Stem Cell PCR Array (SABiosciences). (umich.edu)
  • Stem cells from bone marrow form a number of cell types of the immune and circulatory system. (godandscience.org)
  • If many people donate, then we can type the cells before we freeze and bank them, so that we will know all the immune characteristics. (medicalnewstoday.com)
  • Bone marrow is the home of HSCs that produce all blood cells, including all types of immune cells. (medicalnewstoday.com)
  • Bone marrow transplantation can also be used to treat other disorders, such as immune deficiency disorders. (medicalnewstoday.com)
  • These first 100 days are critical for Ronda's body as the stem cells do most of their work and her immune system begins to rebuild and modulate. (gofundme.com)
  • In all cell populations, older individuals exhibit increasing numbers of senescent cells, perhaps largely due to the progressive decline of the immune system and its growing failure to clear out unwanted or harmful cells. (medworm.com)
  • The bone marrow disease myelofibrosis is stimulated by excessive signaling from vitamin D and immune cells known as macrophages, reveals a Japanese research team. (news-medical.net)
  • By analyzing this model, they found that hematopoietic stem cells were strongly stimulated by vitamin D signaling and grew into immune system cells called macrophages. (news-medical.net)
  • Characteristic features of neuroinflammation in chronic pain conditions include infiltration of immune cells into the PNS [e.g., the sciatic nerve and dorsal root ganglion (DRG)], activation of glial cells such as microglia and astrocytes in the CNS (spinal cord and brain), and production and secretion of pro-inflammatory cytokines and chemokines [TNF, interleukin (IL)-1β, IL-6, CCL2, and CXCL1]. (rutgers.edu)
  • They may also help stop your immune system from attacking the transplanted cells. (drugs.com)
  • HLA protein is made up of six antigens, molecules that stimulate an immune response and help your body identify which cells belong to your body and which do not. (wdxcyber.com)
  • OSTEODEN stemmed from early observations that the immune system, and particularly T cells, was a key player in post-menopausal bone loss. (europa.eu)