A serine-rich sticky protein secreted by MOTHS. Generally, the term refers to silkworm silk gum protein secreted in the middle section of silk gland cells of SILKWORMS, Bombyx mori. Sericin acts as a cement and coating for the two fibroin filaments in a silk strand and is readily soluble in mild alkaline solution.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Periodic casting off FEATHERS; HAIR; or cuticle. Molting is a process of sloughing or desquamation, especially the shedding of an outer covering and the development of a new one. This phenomenon permits growth in ARTHROPODS, skin renewal in AMPHIBIANS and REPTILES, and the shedding of winter coats in BIRDS and MAMMALS.
Amino Acid Sequence
RNA, Transfer, Gly
Family of INSECT VIRUSES containing two subfamilies: Eubaculovirinae (occluded baculoviruses) and Nudibaculovirinae (nonoccluded baculoviruses). The Eubaculovirinae, which contain polyhedron-shaped inclusion bodies, have two genera: NUCLEOPOLYHEDROVIRUS and GRANULOVIRUS. Baculovirus vectors are used for expression of foreign genes in insects.
Usually high-molecular-weight, straight-chain primary alcohols, but can also range from as few as 4 carbons, derived from natural fats and oils, including lauryl, stearyl, oleyl, and linoleyl alcohols. They are used in pharmaceuticals, cosmetics, detergents, plastics, and lube oils and in textile manufacture. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
Sequence Homology, Amino Acid
Small, sacculated organs found within the DERMIS. Each gland has a single duct that emerges from a cluster of oval alveoli. Each alveolus consists of a transparent BASEMENT MEMBRANE enclosing epithelial cells. The ducts from most sebaceous glands open into a HAIR FOLLICLE, but some open on the general surface of the SKIN. Sebaceous glands secrete SEBUM.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
The class Insecta, in the phylum ARTHROPODA, whose members are characterized by division into three parts: head, thorax, and abdomen. They are the dominant group of animals on earth; several hundred thousand different kinds having been described. Three orders, HEMIPTERA; DIPTERA; and SIPHONAPTERA; are of medical interest in that they cause disease in humans and animals. (From Borror et al., An Introduction to the Study of Insects, 4th ed, p1)
Expressed Sequence Tags
Sequence Analysis, DNA
Chromosomes, Artificial, Bacterial
Viral Structural Proteins
Viral proteins that are components of the mature assembled VIRUS PARTICLES. They may include nucleocapsid core proteins (gag proteins), enzymes packaged within the virus particle (pol proteins), and membrane components (env proteins). These do not include the proteins encoded in the VIRAL GENOME that are produced in infected cells but which are not packaged in the mature virus particle,i.e. the so called non-structural proteins (VIRAL NONSTRUCTURAL PROTEINS).
Elements that are transcribed into RNA, reverse-transcribed into DNA and then inserted into a new site in the genome. Long terminal repeats (LTRs) similar to those from retroviruses are contained in retrotransposons and retrovirus-like elements. Retroposons, such as LONG INTERSPERSED NUCLEOTIDE ELEMENTS and SHORT INTERSPERSED NUCLEOTIDE ELEMENTS do not contain LTRs.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Open Reading Frames
Gene Expression Regulation, Developmental
The homologous chromosomes that are dissimilar in the heterogametic sex. There are the X CHROMOSOME, the Y CHROMOSOME, and the W, Z chromosomes (in animals in which the female is the heterogametic sex (the silkworm moth Bombyx mori, for example)). In such cases the W chromosome is the female-determining and the male is ZZ. (From King & Stansfield, A Dictionary of Genetics, 4th ed)
Sequence Homology, Nucleic Acid
Electrophoresis, Polyacrylamide Gel
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Animals, Genetically Modified
Gene Expression Profiling
Inclusion Bodies, Viral
An area showing altered staining behavior in the nucleus or cytoplasm of a virus-infected cell. Some inclusion bodies represent "virus factories" in which viral nucleic acid or protein is being synthesized; others are merely artifacts of fixation and staining. One example, Negri bodies, are found in the cytoplasm or processes of nerve cells in animals that have died from rabies.
Random Amplified Polymorphic DNA Technique
Technique that utilizes low-stringency polymerase chain reaction (PCR) amplification with single primers of arbitrary sequence to generate strain-specific arrays of anonymous DNA fragments. RAPD technique may be used to determine taxonomic identity, assess kinship relationships, analyze mixed genome samples, and create specific probes.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
DNA Transposable Elements
Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
The small RNA molecules, 73-80 nucleotides long, that function during translation (TRANSLATION, GENETIC) to align AMINO ACIDS at the RIBOSOMES in a sequence determined by the mRNA (RNA, MESSENGER). There are about 30 different transfer RNAs. Each recognizes a specific CODON set on the mRNA through its own ANTICODON and as aminoacyl tRNAs (RNA, TRANSFER, AMINO ACYL), each carries a specific amino acid to the ribosome to add to the elongating peptide chains.
Life Cycle Stages
POU Domain Factors
A family of transcription factors characterized by the presence of a bipartite DNA-binding domain known as the POU domain. The POU domain contains two subdomains, a POU-specific domain and a POU-homeodomain. The POU domain was originally identified as a region of approximately 150 amino acids shared between the Pit-1, Oct-1, Oct-2, and Unc-86 transcription factors.
DNA molecules capable of autonomous replication within a host cell and into which other DNA sequences can be inserted and thus amplified. Many are derived from PLASMIDS; BACTERIOPHAGES; or VIRUSES. They are used for transporting foreign genes into recipient cells. Genetic vectors possess a functional replicator site and contain GENETIC MARKERS to facilitate their selective recognition.
Reverse Transcriptase Polymerase Chain Reaction
Chromatography, Ion Exchange
Accumulation of a drug or chemical substance in various organs (including those not relevant to its pharmacologic or therapeutic action). This distribution depends on the blood flow or perfusion rate of the organ, the ability of the drug to penetrate organ membranes, tissue specificity, protein binding. The distribution is usually expressed as tissue to plasma ratios.
Gene Expression Regulation
Promoter Regions, Genetic
An order of the class Insecta. Wings, when present, number two and distinguish Diptera from other so-called flies, while the halteres, or reduced hindwings, separate Diptera from other insects with one pair of wings. The order includes the families Calliphoridae, Oestridae, Phoridae, SARCOPHAGIDAE, Scatophagidae, Sciaridae, SIMULIIDAE, Tabanidae, Therevidae, Trypetidae, CERATOPOGONIDAE; CHIRONOMIDAE; CULICIDAE; DROSOPHILIDAE; GLOSSINIDAE; MUSCIDAE; TEPHRITIDAE; and PSYCHODIDAE. The larval form of Diptera species are called maggots (see LARVA).
Antimicrobial Cationic Peptides
Small cationic peptides that are an important component, in most species, of early innate and induced defenses against invading microbes. In animals they are found on mucosal surfaces, within phagocytic granules, and on the surface of the body. They are also found in insects and plants. Among others, this group includes the DEFENSINS, protegrins, tachyplesins, and thionins. They displace DIVALENT CATIONS from phosphate groups of MEMBRANE LIPIDS leading to disruption of the membrane.
The most abundant form of RNA. Together with proteins, it forms the ribosomes, playing a structural role and also a role in ribosomal binding of mRNA and tRNAs. Individual chains are conventionally designated by their sedimentation coefficients. In eukaryotes, four large chains exist, synthesized in the nucleolus and constituting about 50% of the ribosome. (Dorland, 28th ed)
Arthropods of the class ARACHNIDA, order Araneae. Except for mites and ticks, spiders constitute the largest order of arachnids, with approximately 37,000 species having been described. The majority of spiders are harmless, although some species can be regarded as moderately harmful since their bites can lead to quite severe local symptoms. (From Barnes, Invertebrate Zoology, 5th ed, p508; Smith, Insects and Other Arthropods of Medical Importance, 1973, pp424-430)
Pest Control, Biological
Repetitive Sequences, Nucleic Acid
Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES).
Fushi Tarazu Transcription Factors
A gene silencing phenomenon whereby specific dsRNAs (RNA, DOUBLE-STRANDED) trigger the degradation of homologous mRNA (RNA, MESSENGER). The specific dsRNAs are processed into SMALL INTERFERING RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA-INDUCED SILENCING COMPLEX. DNA METHYLATION may also be triggered during this process.
Genes that encode highly conserved TRANSCRIPTION FACTORS that control positional identity of cells (BODY PATTERNING) and MORPHOGENESIS throughout development. Their sequences contain a 180 nucleotide sequence designated the homeobox, so called because mutations of these genes often results in homeotic transformations, in which one body structure replaces another. The proteins encoded by homeobox genes are called HOMEODOMAIN PROTEINS.
Gene Expression Regulation, Viral
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Body of knowledge related to the use of organisms, cells or cell-derived constituents for the purpose of developing products which are technically, scientifically and clinically useful. Alteration of biologic function at the molecular level (i.e., GENETIC ENGINEERING) is a central focus; laboratory methods used include TRANSFECTION and CLONING technologies, sequence and structure analysis algorithms, computer databases, and gene and protein structure function analysis and prediction.
Green Fluorescent Proteins
Amplified Fragment Length Polymorphism Analysis
Recombinant Fusion Proteins
Nucleic Acid Conformation
Lethal Dose 50
Gene Knockout Techniques
Amino Acyl-tRNA Synthetases
DNA Restriction Enzymes
Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.
Chromatography, High Pressure Liquid
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)
Protein Structure, Secondary
Receptors, Cell Surface
Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.
Olfactory Receptor Neurons
Neurons in the OLFACTORY EPITHELIUM with proteins (RECEPTORS, ODORANT) that bind, and thus detect, odorants. These neurons send their DENDRITES to the surface of the epithelium with the odorant receptors residing in the apical non-motile cilia. Their unmyelinated AXONS synapse in the OLFACTORY BULB of the BRAIN.
A process whereby multiple RNA transcripts are generated from a single gene. Alternative splicing involves the splicing together of other possible sets of EXONS during the processing of some, but not all, transcripts of the gene. Thus a particular exon may be connected to any one of several alternative exons to form a mature RNA. The alternative forms of mature MESSENGER RNA produce PROTEIN ISOFORMS in which one part of the isoforms is common while the other parts are different.
Protein Structure, Tertiary
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Within a eukaryotic cell, a membrane-limited body which contains chromosomes and one or more nucleoli (CELL NUCLEOLUS). The nuclear membrane consists of a double unit-type membrane which is perforated by a number of pores; the outermost membrane is continuous with the ENDOPLASMIC RETICULUM. A cell may contain more than one nucleus. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.
Viral Plaque Assay
Method for measuring viral infectivity and multiplication in CULTURED CELLS. Clear lysed areas or plaques develop as the VIRAL PARTICLES are released from the infected cells during incubation. With some VIRUSES, the cells are killed by a cytopathic effect; with others, the infected cells are not killed but can be detected by their hemadsorptive ability. Sometimes the plaque cells contain VIRAL ANTIGENS which can be measured by IMMUNOFLUORESCENCE.
The biosynthesis of transfer RNA in insects. II. Isolation of transfer RNA precursors from the posterior silk gland of Bombyx mori. (1/1272)The occurrence of precursors to tRNA in the post-polysomal fraction of the posterior silk gland of Bombyx mori was demonstrated by pulse-chase labeling and DNA-RNA hybridization competition experiments. These precursors had molecular sizes ranging from 4S to 5S on polyacrylamide gel electrophoresis. Analysis of the incorporation of the methyl group from [methyl-14C]methionine revealed that a radioactive peak on polyacrylamide gel appeared in the 4.5S region during brief labeling. This suggested that some methylation occurred at the 4.5S precursor step. (+info)
Comparison of Bombyx mori and Helicoverpa armigera cytoplasmic actin genes provides clues to the evolution of actin genes in insects. (2/1272)The cytoplasmic actin genes BmA3 and BmA4 of Bombyx mori were found clustered in a single genomic clone in the same orientation. As a similar clustering of the two cytoplasmic actin genes Ha3a and Ha3b also occurs in another lepidopteran, Helicoverpa armigera, we analyzed the sequence of the pair of genes from each species. Due to the high conservation of cytoplasmic actins, the coding sequence of the four genes was easily aligned, allowing the detection of similarities in noncoding exon and intron sequences as well as in flanking sequences. All four genes exhibited a conserved intron inserted in codon 117, an original position not encountered in other species. It can thus be postulated that all of these genes derived from a common ancestral gene carrying this intron after a single event of insertion. The comparison of the four genes revealed that the genes of B. mori and H. armigera are related in two different ways: the coding sequence and the intron that interrupts it are more similar between paralogous genes within each species than between orthologous genes of the two species. In contrast, the other (noncoding) regions exhibited the greatest similarity between a gene of one species and a gene of the other species, defining two pairs of orthologous genes, BmA3 and HaA3a on one hand and BmA4 and HaA3b on the other. However, in each species, the very high similarities of the coding sequence and of the single intron that interrupts it strongly suggest that gene conversion events have homogenized this part of the sequence. As the divergence of the B. mori genes was higher than that of the H. armigera genes, we postulated that the gene conversion occurred earlier in the B. mori lineage. This leads us to hypothesize that gene conversion could also be responsible for the original transfer of the common intron to the second gene copy before the divergence of the B. mori and H. armigera lineages. (+info)
Active transport of calcium across the isolated midgut of Hyalophora cecropia. (3/1272)1. The net flux of 45Ca from lumen to blood side across the isolated and short-circuited Cecropia midgut was 1-9 +/- 0-2 muequiv. cm-2h-1 in 8 mM Ca and the flux ratio was as high as 56 to 1. 2. The calcium influx was depressed by anoxia; 73% after 30 min. 3. The kinetics of Ca transport were anomalous; the apparent Km varied with Ca concentration from less than 0-2 to greater than 5-6 mM Ca and the apparent Vmax varied from less than 1-3 to greater than 3-3 muequiv. cm-2h-1. 4. The calcium influx showed a delay before the tracer steady state was attained, indicating the existence in the transport route of a calcium pool equivalent to 5-7 muequiv/g. wet weight of midgut tissue. 5 High calcium (16 mM) depressed the short-circuit current and potassium transport from blood to lumen side across the midgut. 6. Calcium depressed magnesium transport, from lumen to blood side across the midgut, and magnesium depressed the calcium transport. 7. Ca transport by the midgut does not regulate the Ca level in the haemolymph in vivo; it merely aids the diffusion of calcium down its electrochemical gradient. However, Ca transport may assist the uptake of the nutrients from the midgut contents. (+info)
Prophenoloxidase-activating enzyme of the silkworm, Bombyx mori. Purification, characterization, and cDNA cloning. (4/1272)Prophenoloxidase-activating enzyme (PPAE) was purified to homogeneity as judged by SDS-polyacrylamide gel electrophoresis from larval cuticles of the silkworm, Bombyx mori. The purified PPAE preparation was shown to be a mixture of the isozymes of PPAE (PPAE-I and PPAE-II), which were eluted at different retention times in reversed-phase high performance liquid chromatography. PPAE-I and PPAE-II seemed to be post translationally modified isozymes and/or allelic variants. Both PPAE isozymes were proteins composed of two polypeptides (heavy and light chains) that are linked by disulfide linkage(s) and glycosylated serine proteases. The results of cDNA cloning, peptide mapping, and amino acid sequencing of PPAE revealed that PPAE is synthesized as prepro-PPAE with 441 amino acid residues and is activated from pro-PPAE by cleavage of a peptide bond between Lys152 and Ile153. The homology search showed 36.9% identity of PPAE to easter, which is a serine protease involved in dorso-ventral pattern formation in the Drosophila embryo, and indicated the presence of two consecutive clip-like domains in the light chain. A single copy of the PPAE gene was suggested to be present in the silkworm genome. In the fifth instar larvae, PPAE transcripts were detected in the integument, hemocytes, and salivary glands but not in the fat body or mid gut. A polypeptide cross-reactive to mono-specific anti-PPAE/IgG was transiently detected in the extract of eggs between 1 and 3 h after they were laid. (+info)
Gene targeting in the silkworm by use of a baculovirus. (5/1272)The Bombyx mori fibroin light (L)-chain gene was cloned and the green fluorescent protein (GFP) gene inserted into exon 7. The chimeric L-chain-GFP gene was used to replace the polyhedrin gene of Autographa californica nucleopolyhedrovirus (AcNPV). This recombinant virus was used to target the L-chain-GFP gene to the L-chain region of the silkworm genome. Female moths were infected with the recombinant virus and then mated with normal male moths. Genomic DNA from their progenies was screened for the desired targeting event. This analysis showed that the chimeric gene had integrated into the L-chain gene on the genome by homologous recombination and was stably transmitted through generations. The chimeric gene was expressed in the posterior silk gland, and the gene product was spun into the cocoon layer. (+info)
Characterization of the 25K FP gene of the baculovirus Bombyx mori nucleopolyhedrovirus: implications for post-mortem host degradation. (6/1272)Mutagenesis experiments on the baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV) using 5-bromo-2'-deoxyuridine generated five mutants with a 'few polyhedra' (FP) phenotype. Sequence analysis of the 25K gene homologue of the BmNPV FP mutants revealed nucleotide substitutions in the coding region. Rescue experiments indicated that the FP phenotype of the BmNPV mutants resulted from mutations in the 25K coding region. Effects of infection by these FP mutants were analysed following injection of the viruses into silkworm (B. mori) larvae. Compared to infection with wild-type virus, infection with each FP mutant resulted in reduced host degradation (liquefaction). The degree to which liquefaction was blocked corresponded to the degree of functional disruption of the 25K gene product and to the extent to which polyhedron production was reduced. Electron microscopy revealed that (1) polyhedron production was reduced, (2) very few virions were occluded and those that were lacked envelopes, and (3) the basal lamina of fat-body tissue was not destroyed by infection and accumulations of virions occurred along the membrane. Typical NPV-induced liquefaction was observed following infection with a polyhedrin deletion mutant, indicating that host degradation was not related to polyhedron production. These results suggest that (1) the 25K gene product is involved in the host degradation process caused by virus infection and (2) the FP phenotype is an indirect result of disruption of the 25K gene; activation or suppression of a specific host or viral gene related to tissue degradation and polyhedron formation may be involved. (+info)
Studies on silk fibroin of Bombyx mori. I. Fractionation of fibroin prepared from the posterior silk gland. (7/1272)1. Fractionation of fibroin prepared from the posterior silk glands of Bombyx mori was carried out. After carboxymethylation of the fibroin, it was fractionated by ammonium sulfate precipitation, Sephadex G-200 gel filtration and DEAE-cellulose column chromatography. 2. The fibroin was composed of at least two protein groups of large molecular size and three or four components of small molecular size, and, in addition, a mixture of proteins ranging in size from about 25,000 to more than 100,000 daltons with almost the same amino acid compositions. 3. The latter proteins contained about 48% glycine, 32% alanine, 11% serine, 4.5% tyrosine, 2% valine, and other minor amino acids. The sum of these main five amino acids accounts for more than 97% of the total amino acid residues of the proteins. 4. The present results indicate major heterogeneity in the molecular size of posterior silk gland fibroin, and, in addition, suggest the possibility of repeating sequences with relatively simple amino acid compositions in major peptide chains of fibroin. (+info)
A TATA element is required for tRNA promoter activity and confers TATA-binding protein responsiveness in Drosophila Schneider-2 cells. (8/1272)In contrast to yeast and mammalian systems, which depend principally on internal promoter elements for tRNA gene transcription, insect systems require additional upstream sequences. To understand the function of the upstream sequences, we have asked whether the Bombyx mori tRNACAla and tRNASGAla genes, which are absolutely dependent on these sequences in vitro, also require them for transcription in vivo. We introduced wild-type and mutant versions of the Bombyx tRNAAla genes into Drosophila Schneider-2 cells and found that the tRNACAla gene is efficiently transcribed and that its transcription depends strongly on the distal segment of its upstream promoter. In contrast, the tRNASGAla gene is inefficiently transcribed, and this inefficiency results from lack of a specific sequence within the distal tRNACAla upstream promoter. This sequence, 5'-TTTATAT-3', is sufficient to increase the activity of the tRNASGAla promoter to that of the tRNACAla promoter. Moreover, promoters containing the 5'-TTTATAT-3' element are stimulated by increased levels of cellular TATA-binding protein. Together these results indicate that, in insect cells, a TATA-like element is specifically required to form functional TATA-binding protein-containing complexes that promote efficient transcription of tRNA genes. (+info)
Experimental immunology Proteomic and immunological identification of two new allergens from silkworm ( Bombyx mori L.) pupae ...
This study explored food allergy caused by eating silkworm (Bombyx mori L.) pupae, a traditionally accepted food and animal feed in East and Southeast Asia, and identified two new allergens by proteomic and immunological methods. Proteins isolated from silkworm pupae were separated by...
Assessing Genetic Diversity in Iranian Native Silkworm (Bombyx mori L.) Strains and Japanese Commercial Lines Using AFLP Markers
Three Iranian native strains and three Japanese commercial lines of the silkworm Bombyx mori were analyzed using amplified fragment length polymorphism (AFLP) markers. A set of ten PstI/TaqI primer combinations amplified a total of 322 bands out of which 251 (%78) were polymorphic. Estimates of Neis gene diversity for all loci in individual strains and commercial lines indicated a higher degree of genetic similarity within Japanese commercial lines than the Iranian native strains. The highest and the least degree of gene diversity were related to the Khorasan Orange strain (h=0.1812) and P107 commercial line (h=0.0804), respectively. The dendrogran constructed using the unweighted pair-group method with arithmitic average based on nais genetic distance revealed two distinct groups as Khorasan native and Japanese commercial lines. The distinct clustering of these two sets of strains and lines reflects differences of the geographical origin and morphological, qualitative and quantitative traits
Analysis of correlation between haemolymph and midgut tissue amylase with commercial characters of silkworm Bombyx mori L. -...
Four mulberry silkworm races viz., Pure Mysore, Nistari, NB4D2 & CSR2 and two hybrid (Pure Mysore × CSR2 and Nistari × NB4D2 ) silkworms were selected for the present study. The specific activity of amylase in the haemolymph as well as midgut tissue was estimated. The qualitative analysis of amylase was carried out by Native-PAGE. The commercial characters viz., fecundity, larval weight, larval duration, cocoon weight, shell weight, shell ratio, filament length, denier and renditta were selected. The results of quantitative analysis were subjected for statistical analysis against selected commercial characters to know the level and kind of correlation between them. The results of statistical analysis clearly showed that haemolymph amylase has highly positive correlation with selected commercial characters except larval duration. In contrast, the midgut amylase indicated highly positive correlation with larval duration only. The zymograms of amylase also exhibited variation among the selected ...
Burst expansion, distribution and diversification of MITEs in the silkworm genome | BMC Genomics | Full Text
Miniature inverted-repeat transposable elements (MITEs) are widespread in plants and animals. Although silkworm (Bombyx mori) has a large amount of and a variety of transposable elements, the genome-wide information of the silkworm MITEs is unknown. We used structure-based and homology approaches to search for MITEs in the silkworm genome. We identified 17 MITE families with a total of 5785 members, accounting for ~0.4% of the genome. 7 of 17 MITE families are completely novel based on the nucleotide composition of target site duplication (TSD) and/or terminal inverted repeats (TIR). Silkworm MITEs were widely and nonrandom distributed in the genome. One family named BmMITE-2 might experience a recent burst expansion. Network and diversity analyses for each family revealed different diversification patterns of the silkworm MITEs, reflecting the signatures of genome-shocks that silkworm experienced. Most silkworm MITEs preferentially inserted into or near genes and BmMITE-11 that encodes a germline
Microbiology Society Journals | A baculovirus isolated from wild silkworm encompasses the host ranges of Bombyx mori...
A baculovirus, named BomaNPV S2, was isolated from a diseased larva of the wild silkworm, Bombyx mandarina. Notably, BomaNPV S2 exhibited a distinguishing feature in that its host range covered that of both Bombyx mori nucleopolyhedrosis virus (BmNPV) and Autographa californica multiple nucleopolyhedrovirus (AcMNPV) in cultured cells. It could replicate in cells of B. mori (Bm5 and BmN), Spodoptera frugiperda (Sf9) and Trichoplusia ni (Tn-5B1-4). However, occlusion-derived virions of BomaNPV S2 in B. mori cells contained only a single nucleocapsid, whereas they contained multiple nucleocapsids in Tn-5B1-4 cells. The complete genome sequence of BomaNPV S2, including predicted ORFs, was determined and compared with the genome sequence of its close relatives. The comparison results showed that most of the BomaNPV S2 genome sequence was shared with BmNPV (BmNPV T3) or BomaNPV S1, but several regions seemed more similar to regions of AcMNPV. This observation might explain why BomaNPV S2 covers the host
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Gopinathan, KP (2012) Welcome to the world of insects! In: CURRENT SCIENCE, 102 (11). p. 1503. Gopinathan, KP (2008) T. Ramakrishnan (1922-2008). In: Current Science, 94 (7). pp. 935-936. Singh, Amit and Singh, Madhuri Kango and Parthasarathy, R and Gopinathan, KP (2007) Larval legs of mulberry silkworm Bombyx mori are prototypes for the adult legs. In: Genesis, 45 (4). pp. 169-176. Dhawan, Sangeeta and Gopinathan, KP (2005) Phylogeny of the Insect Homeobox Gene (Hox) Cluster. In: Genomics Proteomics Bioinformatics, 3 (1). pp. 42-46. Dhawan, Sangeeta and Gopinathan, KP (2004) Molecular cloning and expression pattern of an armadillo homologue from the mulberry silkworm Bombyx mori. In: Gene Expression Patterns, 4 (1). pp. 15-23. Dhawan, Sangeeta and Gopinathan, KP (2003) Expression profiling of homeobox genes in silk gland development in the mulberry silkworm Bombyx mori. In: Development Genes and Evolution, 213 (11). pp. 523-533. Dhawan, Sangeeta and Gopinathan, KP (2003) Cell cycle events ...
Silkworm larvae and cocoon, Bombyx mori, against white background Stock Photo by Lifeonwhite
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Studies on the Mode of Action of the Diapause Hormone in the Silkworm, BOMBYX MORI L., | Journal of Experimental Biology
1. The action of the diapause hormone has been studied by injecting extracts of the heads of male moths or of the brain-suboesophageal ganglion complexes of pupae into pupae expected to produce non-diapause eggs.. 2. The effect of the injection of hormone upon young oocytes is to make them develop into diapause eggs. Older oocytes, which have already acquired non-diapause characters, are not affected.. 3. The hormone is almost completely inactivated when injected on the day of pupation. The hormone is most effective when injected into pupae 2-3 days old, at which stage the ovarioles have started to grow vigorously. It is ineffective 1-2 days before adult emergence, by which time all the oocytes have acquired non-diapause characters.. 4. The hormone is inactivated in all pupae irrespective of whether they are destined to produce diapause eggs or non-diapause eggs. Inactivation of diapause hormone (in contrast to that of juvenile hormone) is partially relieved by exposure to low temperature or by ...
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Silkworm larvae, Bombyx mori, against white background Stock Photo by Lifeonwhite
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Generation of hybrid transgenic silkworms that express Bombyx mori prolyl-hydroxylase alpha-subunits and human collagens in...
Prolyl 4-hydroxylase (P4H) is a heterotetramer enzyme consisting of alpha-subunits (P4Halpha) and beta-subunits (P4Hbeta), and is required for collagen biosynthesis. Previously, we generated transgenic silkworms that produced human type III collagen fragments (mini-collagens) in the posterior silk gland (PSG). However, prolyl 4-hydroxylation did not occur on the mini-collagens, because in spite of an abundant expression of P4Hbeta in PSGs, P4Halpha expression was quite low there, thus resulting in an insufficient activity of P4H. In this study we aimed at generating hybrid transgenic silkworms whose PSGs are capable of producing mini-collagens and enough P4H for their prolyl 4-hydroxylation. Isolated PSGs were bombarded with fibroin L-chain gene promoter-driven vectors containing Bombyx mori P4Halpha (BmP4Halpha) cDNAs and were transplanted into the hemolymphatic cavity. The P4H activity in the PSG cells significantly increased, indicating that the expressed BmP4Halpha formed active tetramers with
LOCUS DF090416 1310704 bp DNA linear CON 26-OCT-2017 DEFINITION Bombyx mori DNA, scaffold: Bm_scaf101, strain: p50T/Dazao. ACCESSION DF090416 BABH01000000 VERSION DF090416.1 DBLINK BioProject:PRJDA20217 BioSample:SAMD00036537 KEYWORDS WGS. SOURCE Bombyx mori (domestic silkworm) ORGANISM Bombyx mori Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Holometabola; Lepidoptera; Glossata; Ditrysia; Bombycoidea; Bombycidae; Bombycinae; Bombyx. REFERENCE 1 (bases 1 to 1310704) AUTHORS Mita,K. and Xia,Q. CONSRTM The international silkworm genome sequencing consortium TITLE Direct Submission JOURNAL Submitted (06-SEP-2007) to the DDBJ/EMBL/GenBank databases. Contact:Kazuei Mita National Institute of Agrobiological Sciences, Insect Genome Research Unit; 1-2 Owashi, Tsukuba, Ibaraki 305-8634, Japan REFERENCE 2 AUTHORS CONSRTM The International Silkworm Genome Consortium TITLE The genome of a lepidopteran model insect, the silkworm Bombyx mori JOURNAL Insect Biochem. Mol. ...
LOCUS DF090445 668521 bp DNA linear CON 26-OCT-2017 DEFINITION Bombyx mori DNA, scaffold: Bm_scaf130, strain: p50T/Dazao. ACCESSION DF090445 BABH01000000 VERSION DF090445.1 DBLINK BioProject:PRJDA20217 BioSample:SAMD00036537 KEYWORDS WGS. SOURCE Bombyx mori (domestic silkworm) ORGANISM Bombyx mori Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Holometabola; Lepidoptera; Glossata; Ditrysia; Bombycoidea; Bombycidae; Bombycinae; Bombyx. REFERENCE 1 (bases 1 to 668521) AUTHORS Mita,K. and Xia,Q. CONSRTM The international silkworm genome sequencing consortium TITLE Direct Submission JOURNAL Submitted (06-SEP-2007) to the DDBJ/EMBL/GenBank databases. Contact:Kazuei Mita National Institute of Agrobiological Sciences, Insect Genome Research Unit; 1-2 Owashi, Tsukuba, Ibaraki 305-8634, Japan REFERENCE 2 AUTHORS CONSRTM The International Silkworm Genome Consortium TITLE The genome of a lepidopteran model insect, the silkworm Bombyx mori JOURNAL Insect Biochem. Mol. ...
Effects of KK-42, an imidazole derivative, on rearing and reproductive performance of Bombyx mori L. during the hot, wet season...
Effects of KK-42, an imidazole derivative, on rearing and reproductive performance of Bombyx mori L. during the hot, wet season - Volume 19 Issue 1 - Kamalendu Banerjee, Debes Chandra Deb
Sequences of two cDNAs encoding silkworm homologues of Drosophila melanogaster squid gene<...
TY - JOUR. T1 - Sequences of two cDNAs encoding silkworm homologues of Drosophila melanogaster squid gene. AU - Li, Feng Qian. AU - Sun, Guan Cheng. AU - Ueda, Hitoshi. AU - Hirose, Susumu. PY - 1995. Y1 - 1995. N2 - The squid (sqd) gene of Drosophila melanogaster encodes a few isoforms of a heterogeneous nuclear (hn) RNA-binding protein. We isolated two types of cDNAs coding for homologues of the Sqd protein from the silkworm Bombyx mori. The two predicted amino acid (aa) sequences are identical up to aa 280 and then diverge. The silkworm and fruit fly proteins share 80% homology in the RNA-binding motif region. These cDNAs detect 2.0-, 1.8- and 1-kb mRNAs in the middle and posterior silk glands.. AB - The squid (sqd) gene of Drosophila melanogaster encodes a few isoforms of a heterogeneous nuclear (hn) RNA-binding protein. We isolated two types of cDNAs coding for homologues of the Sqd protein from the silkworm Bombyx mori. The two predicted amino acid (aa) sequences are identical up to aa 280 ...
Identification of a Novel Strong and Ubiquitous Promoter/Enhancer in the Silkworm Bombyx mori | G3: Genes | Genomes | Genetics
Transgenic techniques offer a valuable tool for determining gene functions. Although various promoters are available for use in gene overexpression, gene knockdown, and identification of transgenic individuals, there is nevertheless a lack of versatile promoters for such studies, and this dearth acts as a bottleneck, especially with regard to nonmodel organisms. Here, we succeeded in identifying a novel strong and ubiquitous promoter/enhancer in the silkworm. We identified a unique silkworm strain whose reporter gene showed strong and ubiquitous expression during the establishment of enhancer trap strains. In this strain, the transposon was inserted into the 5′UTR of hsp90, a housekeeping gene that is abundantly expressed in a range of tissues. To determine whether the promoter/enhancer of hsp90 could be used to induce strong gene expression, a 2.9-kb upstream genomic fragment of hsp90 was isolated (hsp90P2.9k), and its transcriptional activation activity was examined. Strikingly, hsp90P2.9k ...
RCSB PDB - 1BOM: THREE-DIMENSIONAL STRUCTURE OF BOMBYXIN-II, AN INSULIN-RELATED BRAIN-SECRETORY PEPTIDE OF THE...
1BOM: Three-dimensional solution structure of bombyxin-II an insulin-like peptide of the silkmoth Bombyx mori: structural comparison with insulin and relaxin.
Allergenic characterization of 27-kDa glycoprotein, a novel heat stable allergen, from the pupa of silkworm, Bombyx mori<...
TY - JOUR. T1 - Allergenic characterization of 27-kDa glycoprotein, a novel heat stable allergen, from the pupa of silkworm, Bombyx mori. AU - Jeong, Kyoung Yong. AU - Son, Mina. AU - Lee, June Yong. AU - Park, Kyung Hee. AU - Lee, Jae Hyun. AU - Park, Jung Won. PY - 2016/1/1. Y1 - 2016/1/1. N2 - Boiled silkworm pupa is a traditional food in Asia, and patients with silkworm pupa food allergy are common in these regions. Still now only one allergen from silkworm, arginine kinase, has been identified. The purpose of this study was to identify novel food allergens in silkworm pupa by analyzing a protein extract after heat treatment. Heat treated extracts were examined by proteomic analysis. A 27-kDa glycoprotein was identified, expressed in Escherichia coli, and purified. IgE reactivity of the recombinant protein was investigated by ELISA. High molecular weight proteins (above 100 kDa) elicited increased IgE binding after heat treatment compared to that before heat treatment. The molecular ...
RCSB PDB - 3UST: Structure of BmNPV ORF075 (p33) Methods Report Page
3UST: Crystal structure of Bombyx mori nucleopolyhedrovirus ORF75 reveals a pseudo-dimer of thiol oxidase domains with a putative substrate-binding pocket
Serratiopeptidase - Wikipedia
Serratiopeptidase (Serratia E-15 protease, also known as serralysin, serrapeptase, serratiapeptase, serratia peptidase, serratio peptidase, or serrapeptidase) is a proteolytic enzyme (protease) produced by enterobacterium Serratia sp. E-15. This microorganism was originally isolated in the late 1960s from silkworm Bombyx mori L. (intestine), Serratiopeptidase is present in the silkworm intestine and allows the emerging moth to dissolve its cocoon. Serratiopeptase is produced by purification from culture of Serratia E-15 bacteria. Some alternative medicine proponents claim that serratiopeptidase is beneficial for pain and inflammation but existing trials [have been] small and generally of poor methodological quality. Online medical journal Bandolier (specializing in evidence-based thinking about healthcare) published an article (in about 2001) in response to a readers enquiry about serratiopeptidase. After searching PubMed and the Cochrane Library to see if there are any randomised, ...
bombyx mori l complex uk
Adult: Adult (male), Unknown locality, June 14, 2009 (Photo by Paolo Mazzei) Enterobacter, pathogenicity, toxin complex, Bombyx mori INTRODUCTION A bacterial strain was isolated from flacherie-diseased silkworms in 1967 (Ono, Enomoto and Iwanami 1967 ) â ¦ It shows high degradation activity during the pre-pupa period, when the silk gland undergoes apoptosis and remodeling. It is a rare, much sought after silkworm extract used to energize the body. Development. In the silkworm Bombyx mori, morphological variations associated with the E complex, an analogue of the Drosophila melanogaster bithorax complex, mainly determine the shape and number of prolegs on abdominal segments. The extract from R. rosea is reported to extend the lifespan of yeast, nematode, and fruit fly. Dev Biol. Check out what 2,211 people have written so far, and share your own experience. Kaplan, in Polymer Science: A Comprehensive Reference, 2012. 3 â 5, 400372 Cluj- Napoca, Romania Phenotypic effects induced by knock-down ...
Silkworm Eggs (Bombyx mori) | Carolina.com
Bombyx mori. For hatching silkworms. Life cycle takes about 6 to 8 weeks after eggs hatch. Silkworms require fresh mulberry leaves or item #143966 Silkworm Artificial Dry Diet (not included). With instructions.
Rab14 from Bombyx mori (Lepidoptera: Bombycidae) shows ATPase activity
PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.
KAIKObase: An integrated silkworm genome database and data mining tool by Michihiko Shimomura, Hiroshi Minami et al.
Background: The silkworm, Bombyx mori, is one of the most economically important insects in many developing countries owing to its large-scale cultivation for silk production. With the development of genomic and biotechnological tools, B. mori has also become an important bioreactor for production of various recombinant proteins of biomedical interest. In 2004, two genome sequencing projects for B. mori were reported independently by Chinese and Japanese teams; however, the datasets were insufficient for building long genomic scaffolds which are essential for unambiguous annotation of the genome. Now, both the datasets have been merged and assembled through a joint collaboration between the two groups. Description: Integration of the two data sets of silkworm whole-genome-shotgun sequencing by the Japanese and Chinese groups together with newly obtained fosmid- and BAC-end sequences produced the best continuity (~3.7 Mb in N50 scaffold size) among the sequenced insect genomes and provided a high degree
A New Genus, Valvaribifidum (Lepidoptera, Bombycidae), with Description of a New Species from China
Florida Entomologist contains contributions on all aspects of basic and applied entomological science from all geographic regions.
A novel antibacterial peptide family isolated from the silkworm, Bombyx mori | Biochemical Journal
Three structurally related and novel antibacterial peptides have been isolated from the haemolymph of the silkworm, Bombyx mori, immunized with Escherichia coli. These peptides were 32 amino acids long and characteristically rich in proline residues. A unique threonine residue in each peptide was O-glycosylated and the modification seemed to be important for expression of antibacterial activity. The primary structure and antibacterial character of the novel peptides resemble those of abaecin (41% identity in amino acid sequence), an antibacterial peptide of the honeybee, although abaecin is not O-glycosylated. Incubation of the novel peptides with a liposome preparation caused leakage of entrapped glucose under low-ionic-strength conditions, suggesting that a target of the peptides is the bacterial membrane. We propose the name lebocin for the novel peptide family isolated from B. mori. ...
Items where Subject is Genetics - [email protected]
Sackton, T.B. and Corbett-Detig, R.B. and Nagaraju, J. and Vaishna, L. and Arunkumar, K.P. and Hartl, D.L.. (2014) Positive selection drives faster-Z evolution in silkmoths. Evolution, 68 (8). pp. 2331-2342. ISSN 0014-3820 Sah, N.K. and Taneja, T.K. and Pathak, Niteen and Begum, R. and Athar, M. and Hasnain, S.E. (1999) The baculovirus antiapoptotic p35 gene also functions via an oxidant-dependent pathway. Proceedings of the National Academy of Sciences, 96 (9). pp. 4838-4843. ISSN 0027-8424 Sharma, A. and Niphadkar, M.P. and Kathirvel, P. and Nagaraju, J. and Singh, Lalji (1999) Brief communication. DNA fingerprint variability within and among the silkworm Bombyx mori varieties and estimation of their genetic relatedness using Bkm-derived probe. Journal of Heredity, 90 (2). pp. 315-319. ISSN 1471-8505 Sharma, G. and Upadhyay, S. and Srilalitha, M. and Nandicoori, V.K. and Khosla, Sanjeev (2015) The interaction of mycobacterial protein Rv2966c with host chromatin is mediated through non-CpG ...
Indexed Papers | BioTech
2020 Spider (Linothele megatheloides) and silkworm (Bombyx mori) silks: Comparative physical and biological evaluation Yang, Yuejiao; Greco, Gabriele; Maniglio, Devid; Mazzolai, Barbara; Migliaresi, Claudio; Pugno, Nicola; Motta, Antonella MATERIALS SCIENCE AND ENGINEERING. C, BIOMIMETIC MATERIALS, SENSORS AND SYSTEMS, v. 2019, (2020), p. 110197. - DOI:
Bombyx mori L.. K M Ponnuvel*, K Ashok Kumar, D Velu, P Somasundaram, R K Sinha and C K Kamble. Biotechnology Laboratory, Central Sericulture Germplasm Resources Centre, Thally Road, Hosur 635 109, India. Received 12 May 2006; revised 18 June 2007; accepted 6 October 2007. N-terminal amino acid sequence of esterase gene was blast searched with Bombyx mori EST (expressed sequence tag) database. EST clone fbpv0006 showed 95% homology with N-terminal sequence of esterase protein. Further, the genomic organization of exons and introns were identified using the EST clone sequence. The results on genomic organization of esterase gene indicated that the blood esterase gene possesses two exons with varying length of 192 and 524 bp, and a long intron of 2124 bp length present between these two exons. The primers were designed to the intron and exon regions and the fragments were PCR amplified using genomic DNA from silk moth as template. The amplicon showed a mol wt 799 bp in the intron region and 547 bp ...
PLOS Genetics: Precocious Metamorphosis in the Juvenile Hormone-Deficient Mutant of the Silkworm, Bombyx mori
Author Summary The number of larval instars in insects varies greatly across insect taxa and can even vary at the intraspecific level. However, little is known about how the number of larval instars is fixed in each species or modified by the environment. The silkworm, Bombyx mori, provides a unique bioresource for investigating this question, as there are several
Suture Riversilk™ (Silk) | Riverpoint Medical
Silk is a non-absorbable braided surgical suture composed of an organic protein called fibroin. The protein is derived from the domesticated species Bombyx mori (B mori) of the family Bombycidae. Get a quote today.
Cloning and rescue of the genome of Bombyx mori bidensovirus, and characterization of a recombinant virus | Virology Journal |...
Bombyx mori bidensovirus (BmBDV), which belongs to the Bidnaviridae family established by the International Committee on Taxonomy of Viruses in 2011, was the first bidensovirus identified in insects. The structure of BmBDV is similar to that of parvoviruses, while its replication is similar to that of adenoviruses. Although BmBDV has the potential to be used as a tool in biological pest control and as an expression vector, virus rescue has been a bottleneck in the application of this virus. In this study, we constructed a full-length genomic clone of BmBDV and showed that its terminal structure was restored. A recombinant BmBDV that expressed the green fluorescence protein (GFP) gene was constructed. Then, BmN cells, which are an ovarian cell line, were co-transfected with the linearized genome using continuous culture and expanded cell culture methods. The results showed that the GFP gene was expressed successfully, and that cell lesions occurred in virus-infected cells.
diapause hormone Summary Report | CureHunter
diapause hormone: from embryonic stage of Bombyx mori; contains 24-amino acid residues; amino acid sequence given in first source
CiNii Articles - ABE Hiroaki
Identification of novel random amplified polymorphic DNAs (RAPDs) on the W chromosome of the domesticated silkworm, Bombyx mori, and the wild silkworm, B. mandarina, and their retrotransposable elementrelated nucleotide sequences （1998 ...
Genomic boost to protect Assams muga silkworm | Business Standard News
Read more about Genomic boost to protect Assams muga silkworm on Business Standard. A snapshot of the muga silkworm genome decoded by Indian scientists offers vital clues for imparting disease-resistance to help conserve the economically important insect which spins the distinctive golden yellow fabric synonymous with Assam.
Corries Samia Ghadie has given birth to a baby boy | Mother&Baby
Samia and Sylvain - who are preparing to tie the knot next year - first met when the brunette beauty was partnered with the professional skater on Dancing On Ice in 2013.. And Samia recently admitted shes going to miss being pregnant despite the fact shes been suffering with back pain.. She explained: My hair has been in great condition and my nails have been so strong due to all the pregnancy hormones flying around. Even my skin seems to have been better!. I think thats due to the fact that you have double the amount of blood in your body when youre pregnant which supposedly gives you that famous pregnancy glow people talk about!. Story: ©BANG Showbiz. Photo: MCPIX/REX Shutterstock. ...
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silkworms « Ewephoric Fibers
More fun silkworm trivia from Weaving Todays BeWeave It column!. Imagine flowing down the aisle in a darkened room in a silk wedding dress that glows red, orange, and green under a UV light. Scientists in Japan have been experimenting with breeding mutant silkworms that produce silk that glows under a UV light. These silkworms have had genetic material from other organisms inserted into their genomes so they produce a silk with fluorescent qualities. The transgenic critters have a red glowing protein from Discosoma corals, orange from the Fungia concinna coral, or a green fluorescent protein from jellyfish. . . . → Read More: Silkworm Trivia. ...
Cynthia Bourgealt: Encountering the Wisdom Jesus
Modern day mystic, Episcopal priest, writer, and internationally known retreat leader, Cynthia Bourgeault divides her time between solitude at her seaside hermitage in Maine, and a demanding schedule traveling globally to teach and spread the recovery of the Christian contemplative and Wisdom path.. She has been a long-time advocate of the meditative practice of Centering Prayer and has worked closely with fellow teachers and colleagues including Thomas Keating, Bruno Barnhart, and Richard Rohr. Cynthia has actively participated in numerous inter-spiritual dialogues and events with luminaries and leaders such as A.H. Almaas, Kabir Helminski, Swami Atmarupananda, and Rami Shapiro.. Cynthia is a member of the GPIW (Global Peace Initiative for Women) Contemplative Council and recipient of the 2014 Contemplative Voices award from Shalem Institute. She is a founding Director of both The Contemplative Society and the Aspen Wisdom School. She continues to contribute to The Contemplative Society in her ...
A Second-Generation Integrated Map of the Silkworm Reveals Synteny and Conserved Gene Order Between Lepidopteran Insects |...
BOMBYX mori, the domesticated silkworm, is one of the most genetically well-studied insects, with 246 mutations that have been sorted into 27 linkage groups (LGs) (Banno et al. 2005). Genome projects and related work are underway using B. mori as a model organism for Lepidoptera, the most serious group of agricultural pests (for recent review, see Goldsmith et al. 2004). Large-scale sequencing projects of expressed sequence tags (ESTs) (Mita et al. 2003; Cheng et al. 2004) and whole-genome shotgun (WGS) sequences (Mita et al. 2004; Xia et al. 2004) have been performed, and our knowledge of silkworm genes and genome sequence has dramatically increased. However, basic genome research on this insect is still far behind compared with other model organisms such as Drosophila melanogaster, and assignment of fundamental information such as genome sequences, ESTs, BAC contigs, mutant phenotypes, and chromosomal locations on detailed linkage maps is an urgent priority.. Two preliminary molecular linkage ...
Silkworms Sylvia A. Johnson - CTValley Bio
1982. Sylvia A. Johnson. Humans have been raising silkworms for their precious silk for thousands of years. Because of its long association with man, the silkworm moth is actually …
Bombyx mori BMCHIR1protein Summary Report | CureHunter
Bombyx mori BMCHIR1protein: is a chitinase-like gene induced by 20-Hydroxyecdysone in Silkworm; amino acid sequence in first source
KEEPER OF THE SNAILS: Silkworm Housing Crisis
Despite Hodmandod Minors somewhat nonchalant attitude to sericulture (throwing in a bit of silkworm diet after being nagged on the phone, then ramming down the lid and running away fast) there were surprisingly few silkworm casualties on my return. The night I got back I spent until midnight picking out withered grey corpses, putting them on fresh paper and grating in more food. Altogether there were about six - mostly in the box that was streaming with condensation - but there have been more casualties since. At one stage there seemed to be so many corpses every time I looked that I was thinking that my little colony was suffering from a plague (like the one that afflicted most of Europe in the nineteenth century) and could understand how desperate the sericulturists must have felt as generation after generation of silkworms succumbed no matter what anyone did ...
Whole-cell recording from Kenyon cells in silkmoths - Fingerprint - Johns Hopkins University
Cynthia Robinson Weight and Height, Size | Body measurements
Many people ask about Cynthia Robinson Height and Weight 2016, size, age, body statistics and measurements. Cynthia Robinson is an American Trumpet player, her
A BEATLES HARD-DIES SITE: Beatles Wives - 1: Cynthia Powell/Lennon
August 1965: John writing to Cynthia during The Beatles American Tour. ...what he said about it. Its not that much bother really, is it? When you think about it - cause Im sure Dot [the Lennons housekeeper] and Lil [Cynthias mother] and Bennigs, Tommy, Jordy, etc. can understand something as simple as us wanting to be alone for a day - I dont mean Julian - I mean dont pack him off to Dots or anywhere - I really miss him as a person now - do you know what I mean, hes not so much The Baby or My Baby anymore, hes a real living part of me now, you know hes Julian and everything and I cant wait to see him, I miss him more then Ive ever done before - I think its been a slow process my feeling like a real father! I hope all this is clear and understandable. I spend hours in dressing rooms and things thinking about the times Ive wasted not being with him - and playing with him - you know I keep thinking of THOSE stupid bastard times when I keep reading bloody newspapers and other ...
Cynthia Thompson | Information Management
Cynthia Thompson is the Business Development Manager for SGP International, a leading developer of departmental data reconciliation and custom designed data management solutions. Thompson can be reached at (212) 685-0819 or at [email protected]
Cynthia Nixon | Cynthia Nixon had hair worries for wedding | Contactmusic.com
Cynthia Nixons wife was worried about her being bald on their wedding day.The former Sex and the City actress shaved her head for her role as a cancer sufferer in the stage play Wit before her May nuptials to long-term...
Dr. Samia Malik, MD - Tampa, FL - Neurology | Healthgrades.com
Visit Healthgrades for information on Dr. Samia Malik, MD Find Phone & Address information, medical practice history, affiliated hospitals and more.
... from Chinese Bombyx mandarina and paternal inheritance of Antheraea proylei mitochondrial DNA" (PDF). Molecular ... The silkworm is the larva or caterpillar of the domestic silkmoth, Bombyx mori (Latin: "silkworm of the mulberry tree"). It is ... 2004). "The genome sequence of silkworm, Bombyx mori". DNA Research. 11 (1): 27-35. doi:10.1093/dnares/11.1.27. PMID 15141943. ... Many other Lepidoptera produce cocoons, but only a few-the Bombycidae, in particular the genus Bombyx, and the Saturniidae, in ...
Bombyx mori. An insect two-hybrid (I2H) system was developed in a silkworm cell line from the larva or caterpillar of the ... domesticated silk moth, Bombyx mori (BmN4 cells). This system uses the GAL4 BD and the activation domain of mouse NF-κB P65. ...
Fabricius, Johan Christian (1793). "Bombyx". Entomologia systematica emendata et aucta. Vol 3. Part 1. Copenhagen: C. G. Proft ...
Genetically modified animal
Xu J, Dong Q, Yu Y, Niu B, Ji D, Li M, Huang Y, Chen X, Tan A (August 2018). "Bombyx mori". Proceedings of the National Academy ... Silkworm, the larvae stage of Bombyx mori, is an economically important insect in sericulture. Scientists are developing ... Xu H, O'Brochta DA (July 2015). "Advanced technologies for genetically manipulating the silkworm Bombyx mori, a model ...
Bombyx Mori (2011). GOLDFISH (2013). The cunning little vixen, an opera by Leoš Janáček at the Bergen National Opera, Norway ( ... 2002 What Good Would the moon be 2004 Armide 2003 Shaker 2006 Armide 2006 Rushes 2007 Hydra 2007 Trout 2008 Toros 2010 Bombyx ...
Bombyx in Merriam Webster. P.C.-Rougeot, P. Viette (1978). Guide des papillons nocturnes d'Europe et d'Afrique du Nord. ...
Bombyx in Merriam Webster. Aristotle, hist.an. 5,19. 551b 13ff. Pliny the Elder, Naturalis historia 11, 26.. ...
ISBN 0-7817-5056-3. Harano T (1972). "New diaphorases from Bombyx silkworm eggs. NADH/NADPH cytochrome c reductase activity ...
This has been demonstrated in Bombyx mori. In silkworms, there is good evidence that fertile sperm are unable to fertilise if ...
... s from Bombyx mori, a kind of cultivated silkworm, are the most widely investigated silks. Silks derived from Bombyx mori ... The best-known silk is obtained from the cocoons of the larvae of the mulberry silkworm Bombyx mori reared in captivity ( ... Silk fibers from the Bombyx mori silkworm have a triangular cross section with rounded corners, 5-10 μm wide. The fibroin-heavy ... "Fine organization of Bombyx mori fibroin heavy chain gene". Nucleic Acids Research. 28 (12): 2413-2419. doi:10.1093/nar/28.12. ...
List of sequenced animal genomes
Bombyx mori". DNA Research. 11 (1): 27-35. doi:10.1093/dnares/11.1.27. PMID 15141943. Wan F, Yin C, Tang R, Chen M, Wu Q, Huang ... Bombyx mori Strain:p50T, moth (domestic silk worm) (2004) Cydia pomonella, codling moth (2019) Danaus plexippus, butterfly ( ...
Stazione Bacologica Sperimentale
120 strains of Bombyx mori. The institution owns a mulberry field, also used for experiments, that provides the leaves ... necessary for the rearing and breeding of the various Bombyx strains. Mario Tirelli. ...
List of organisms by chromosome count
Bombyx mori) 56 This is for the species mulberry silkworm, B. mori (2n=56). Probably more than 99% of the world's commercial ... Yoshido A, Yasukochi Y, Sahara K (2011). "Samia cynthia versus Bombyx mori: comparative gene mapping between a species with a ... "The Bombyx mori karyotype and the assignment of linkage groups". Genetics. 170 (2): 675-85. doi:10.1534/genetics.104.040352 ...
The most notable of these is the silkworm, the larva of the domesticated moth Bombyx mori. It is farmed for the silk with which ... Not all silk is produced by Bombyx mori. There are several species of Saturniidae that also are farmed for their silk, such as ... Bombyx mori), for its silk Wax moths (Galleria mellonella, Achroia grisella), pests of bee hives Duponchelia fovealis, a new ...
Linnaeus first described Phalaena bombyx fascelina in 1758. Arthur Gardiner Butler first created the genus Dicallomera in 1881 ...
Mir-277 microRNA precursor family
"Identification and characteristics of microRNAs from Bombyx mori". BMC Genomics. 9: 248. doi:10.1186/1471-2164-9-248. PMC ...
External morphology of Lepidoptera
Bombyx mandarina is an example with bipectinate antennae. Some moths have knobbed antennae akin to those of butterflies, ...
Slow bee paralysis virus
Bombyx mori) through Varroa destructor mite infestations. The virus causes paralysis in the front two pairs of legs of adult ...
Tsusué M (April 1971). "Studies on sepiapterin deaminase from the silkworm, Bombyx mori. Purification and some properties of ...
The species Bombyx mori has been domesticated to the point where it is completely dependent on mankind for survival. A number ... The most prominent example is that of the domesticated silkworm moth (Bombyx mori), the larvae of which make their cocoons out ... Goldsmith M. R.; T. Shimada; H. Abe (2005). "The genetics and genomics of the silkworm, Bombyx mori". Annual Review of ... Yoshitake, N. (1968). "Phylogenetic aspects on the origin of Japanese race of the silkworm, Bombyx mori". Journal of ...
Structure of Bombyx mori Chemosensory Protein 1 in solution. Arch Insect Biochem Physiol. 2007; 66: 135-145. 8. Tomaselli S, ... 6. Jansen S, Zídek L, Löfstedt C, Picimbon JF, Sklenar V. 1H, 13C, and 15N resonance assignment of Bombyx mori chemosensory ... Molecular evidence of RNA editing in Bombyx chemosensory protein family. PLoS ONE 2014; 9: e86932. 10. Xuan N, Rajashekar B, ... Structural components of chemosensory protein mutations in the silkworm moth, Bombyx mori. Agri Gene 2016; 2: 53-58. 11. Xuan N ...
Pippa appears as Epicoene, a slave to Harpy, who seeks to escape her clutches with Bombyx. Bombyx responds positively until she ... Quine appears in the Bombyx Mori as Bombyx, an aspiring author whose genius is undisputed, unappreciated, and unsubstantiated, ... Strike soon deduces Bombyx Mori is a metaphor for someone else's life and Owen was intended to be the antagonist rather than ... She is the only person in Quine's life who does not appear in the Bombyx Mori manuscript, and it is implied that she is the ...
History of silk
The species was identified as Bombyx mori, the domesticated silkworm. Fragments of a primitive loom can also be seen from the ... Pliny the Elder notably accurately determined where silk came from; speaking of the bombyx or silk moth, he wrote in his ... the Bombyx mandarina and its domesticated descendant B. mori. Chinese sources claim the existence of a machine to unwind ...
List of edible insects by country
Xylotrupes gideon Gryllotalpa africana Bombyx mori Tessaratoma quadrata Hierodura sp. ? Vespa spp. Hydrophilus affinis ... Isoptera Macrotermes gilvus Lepidoptera Bombyx mori Erionata thrax thrax Omphisa fuscidentalis Homoptera Chremistica sp. ... Bombyx mori (silkworm pupa) Darthula hardwickii Udonga montana Insect species eaten in Indonesia: Hyblaea puera (teak ... Bombyx mori (silkworm pupa) Oecophylla smaragdina (weaver ant) Lethocerus indicus (giant water bug) Heterometrus longimanus ( ...
Tanaka, Y; Hua, Y; Roller, L; Tanaka, S (July 2002). "Corazonin reduces the spinning rate in the silkworm, Bombyx mori". ... Bombyx mori and the cricket, Gryllus bimaculatus, as a factor inducing dark color in an albino strain of the locust, Locusta ... Bombyx mori), corazonin reduces the spinning rate of silk. Veenstra, JA (3 July 1989). "Isolation and structure of corazonin, a ... the cricket Gryllus bimaculatus and the silkworm Bombyx mori and is now known to be the most common form of this peptide. [His( ...
Helicoverpa zea nudivirus 2
Chen HQ, Chen KP, Yao Q, Guo ZJ, Wang LL (December 2007). "Characterization of a late gene, ORF67 from Bombyx mori ... Hz2V035 is most similar to Bombyx mori thymidlylate synthase and is likely involved in the synthesis of dTMP precursors from ... Hz2V007 is very similar to the Bombyx mori carboxylesterase (COE) and, to a lesser degree, Anopheles gambiae juvenile hormone ... Acharya A, Gopinathan KP (August 2002). "Characterization of late gene expression factors lef-9 and lef-8 from Bombyx mori ...
It is also the sex pheromone in the wild silk moth (Bombyx mandarina). Discovered by Adolf Butenandt in 1959, it was the first ... Butenandt named the substance after the moth's Latin name Bombyx mori. In vivo it appears that bombykol is the natural ligand ... Kuwahara, Yasumasa (1984). "Flight Time of Bombyx mandarina Males to a Pheromone Trap Baited with Bombykol". Applied Entomology ... Bombyx mori, sex pheromone". Proceedings of the National Academy of Sciences. 101 (23): 8631-6. Bibcode:2004PNAS..101.8631M. ...
The best-known species is Bombyx mori (Linnaeus) or silkworm, native to northern China and domesticated for millennia. Another ... Amusaron Anticla Walker, 1855 Bivincula Bivinculata Bombyx Colla Dalailama Elachyophtalma Epia Ernolatia Gastridiota ... well-known species is Bombyx mandarina, also native to Asia. The family was recently severely restricted, and currently ...
There is one species in this genus currently recognised: Bombyx mori bidensovirus. As the name suggests this virus infects ... ". "Li G, Hu Z, Guo X, Li G, Tang Q, Wang P, Chen K, Yao Q Identification of Bombyx mori Bidensovirus VD1-ORF4 Reveals a Novel ... Bombyx mori, the silkworm. The virions are icosahedral, non enveloped and ~25 nanometers in diameter. They contain two ...
Bombyx mori - Wikipedia
Bombyx mori from Chinese Bombyx mandarina and paternal inheritance of Antheraea proylei mitochondrial DNA" (PDF). Molecular ... The silkworm is the larva or caterpillar of the domestic silkmoth, Bombyx mori (Latin: "silkworm of the mulberry tree"). It is ... 2004). "The genome sequence of silkworm, Bombyx mori". DNA Research. 11 (1): 27-35. doi:10.1093/dnares/11.1.27. PMID 15141943. ... Many other Lepidoptera produce cocoons, but only a few-the Bombycidae, in particular the genus Bombyx, and the Saturniidae, in ...
annexin B13 [Bombyx mori] - Protein - NCBI
annexin B13 [Bombyx mori] annexin B13 [Bombyx mori]. gi,112983539,ref,NP_001036881.1, ... Large scale full-length cDNA sequencing reveals a unique genomic landscape in a lepidopteran model insect, Bombyx mori. [G3 ( ... Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis.. Ou J, Deng HM, Zheng SC, ... Large scale full-length cDNA sequencing reveals a unique genomic landscape in a lepidopteran model insect, Bombyx mori.. ...
Bombyx mori (Silk moth)
Bombyx - Wikipedia
Bombyx horsfieldi (Moore, 1860) Bombyx huttoni Westwood, 1847 Bombyx incomposita van Eecke, 1929 Bombyx lemeepauli Lemée, 1950 ... wild silk moth Bombyx mori (Linnaeus, 1758) - domestic silk moth Bombyx rotundapex Miyata & Kishida, 1990 Bombyx shini Park and ... Bombyx is the genus of true silk moths or mulberry silk moths of the family Bombycidae, also known as silkworms, which are the ... Conservation Status of Silkworm (Bombyx mori) Genetic Resources in the World. Food and Agriculture Organization. v t e. ...
Bombyx lemeepauli - Wikipedia
Bombyx lemeepauli is a species of Bombycidae in the genus Bombyx. It was described by Albert Marie Victor Lemée in 1950. It is ... "Taxonomy Browser: Bombyx lemeepauli". Barcode of Life Data System. Retrieved November 6, 2018. CS1 maint: discouraged parameter ... link) "Taxonomy Browser: Bombyx lemeepauli". Barcode of Life Data System. Retrieved November 6, 2018. CS1 maint: discouraged ...
Article Metrics - Nucleotide sequence of Bombyx mori L. tRNA 1 Gly | Nature
Silkworm Eggs (Bombyx mori) | Carolina.com
Bombyx mori. For hatching silkworms. Life cycle takes about 6 to 8 weeks after eggs hatch. Silkworms require fresh mulberry ... Silkworm Eggs (Bombyx mori). 2 Items Silkworm Eggs (Bombyx mori) is rated 3.7 out of 5 by 6. ... Bombyx mori. For hatching silkworms. Life cycle takes about 6 to 8 weeks after eggs hatch. Silkworms require fresh mulberry ... Bombyx mori. For hatching silkworms. Life cycle takes about 6 to 8 weeks after eggs hatch. Silkworms require fresh mulberry ...
Uncharacterized protein - Bombyx mori (Silk moth)
Silkworm (Bombyx mori) Farm Kit | Carolina.com
Lead your students through the life cycle of a silkworm, Bombyx mori. This interactive kit even helps them create their own ... Grade K and up. Lead your students through the life cycle of a silkworm, Bombyx mori. This interactive kit even helps them ... Grade K and up. Lead your students through the life cycle of a silkworm, Bombyx mori. This interactive kit even helps them ...
Mass spider silk production through targeted gene replacement in Bombyx mori | PNAS
Mass spider silk production through targeted gene replacement in Bombyx mori. Jun Xu, Qinglin Dong, Ye Yu, Baolong Niu, ... Mass spider silk production through targeted gene replacement in Bombyx mori. Jun Xu, Qinglin Dong, Ye Yu, Baolong Niu, ... The silkworm, Bombyx mori, has been the most well known silk producer for thousands of years and has been considered an ideal ... 2012) N-Terminal domain of Bombyx mori fibroin mediates the assembly of silk in response to pH decrease. J Mol Biol 418:197-207 ...
BOMBYX DISPAR | SCQ
... to gobble your Bombyx pre-emergence since on average 1 bird eats 2 eggs a minute in a 16-hour day 192,000 Bombyx gone Bombyx ... base a character on the moth called Bombyx dispar you could easily set your story in Count Wodzickis garden 1848 Your Bombyx ... BOMBYX DISPAR by brianwillems If you were to base a character. on the moth called Bombyx dispar. you could easily set your ... Birds! to gobble your Bombyx pre-emergence. since on average 1 bird eats 2 eggs a minute. in a 16-hour day 192,000 Bombyx gone ...
Strange Horizons - Bombyx mori By Kerstin Hall
Once upon a time there lived a boy-girl named Cynae. After a series of difficulties, she came to live in a small house in the woods.. Here, our first challenge. Do we address Cynae as she or he? Really, both or neither would be more accurate. More accurate still: it doesnt matter. More accurate still: why does it matter?. And yet, the impasse remains. It would be too inelegant to avoid pronouns altogether, as in Cynae came to live in a small house in the woods. The option to adopt zie is tempting, but will draw attention, and in certain contexts there remains something uncomfortable in addressing a single person as they. After some anxiety, we come to a reasoned decision. There are enough stories where the principal character is a he; thus we will use she.. In the woods there is a house. An old house, a broken-down house, a quiet house. Fine-leaved creepers snarl over the crumbling walls, their waxy fingers tangling, pressing against the window panes and inching down the chimney. This old house ...
A cysteine protease encoded by the baculovirus Bombyx mori nuclear polyhedrosis virus. - PubMed - NCBI
Bombyx | Harvard Catalyst Profiles | Harvard Catalyst
"Bombyx" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject Headings) ... The family contains a single species, Bombyx mori from the Greek for silkworm + mulberry tree (on which it feeds). A native of ... This graph shows the total number of publications written about "Bombyx" by people in Harvard Catalyst Profiles by year, and ... Below are the most recent publications written about "Bombyx" by people in Profiles. ...
The Expression, Purification, and Characterization of a Ras Oncogene (Bras2) in Silkworm (Bombyx mori)
... properties and to characterize the Bras2 present in the silkworm Bombyx mori. The cDNA of BmBras2 from Bombyx mori consists of ... The mRNA of Bombyx mori Bras2 has 1412 bp. The open reading frame contains 603 bp, which encodes 200 amino acid residues. This ... Bombyx mori has a well-studied genetic background and high developmental synchronization. Because it is susceptible to nuclear ... The Expression, Purification, and Characterization of a Ras Oncogene (Bras2) in Silkworm (Bombyx mori). Zhengbing Lv,1,2 Tao ...
Back to Bombyx - Hackteria Wiki
Microscopic Studies of Bombyx mori David Walker, UK. Material Properties and Methods. * Polyol-Silk Bioink Formulations as Two- ... Pdf of Materials fabrication from Bombyx mori silk fibroin, Nat Protoc. 2011 Sep 22;6(10):1612-31. doi: 10.1038/nprot.2011.379. ... SilkDB for the silkworm, Bombyx mori from Scripps and , genomic data including functional annotation of genes, gene products ... The dissolution conditions seem to be different between the domesticated Bombyx, and other wild strains.. ...
Bombyx mori BMCHIR1protein Summary Report | CureHunter
Remediated Sequence - 4EFQ: Bombyx mori lipoprotein 7 - platinum derivative at 1.94 A resolution Sequence Report Page
Critical analysis of correlation and heritability phenomenon in the silkworm, Bombyx mori (Lepidoptera: Bombycidae)
Jayaswal K.P., Singh T. and Subba Rao G. (1991) Effect of female pupal weight on fecundity of mulberry silkworm Bombyx mori. ... Yan L.L. (1983) The estimates of heritability of pupal weight, cocoon weight and number of eggs laid in the silkworm (Bombyx ... Singh, T. , Bhat, M. and Khan, M. (2011) Critical analysis of correlation and heritability phenomenon in the silkworm, Bombyx ... Singh T., Jayaswal K.P. and Subba Rao G. (1992a) Correlation studies between some breeding parameters of silkworm, Bombyx mori ...
Bombyx mori densovirus 1 capsid - Stock Image - C014/5096 - Science Photo Library
This virus infects crustaceans and insects, especially the silkworm (Bombyx mori). ... Bombyx mori densovirus 1 (BmDNV-1), molecular model. This virus infects crustaceans and insects, especially the silkworm ( ... Bombyx mori). In viruses, the capsid is the protein shell that encloses the genetic material. A capsid consists of subunits ...
Bombyx cysteine proteinase inhibitor Summary Report | CureHunter
Bombyx cysteine proteinase inhibitor (BCPI) from the hemolymph of Bombyx mori; alpha and beta are two forms differing only in ... cysteine proteinase inhibitor, Bombyx; BCPI-alpha protein, Bombyx; BCPI-beta protein, Bombyx ... Bombyx cysteine proteinase inhibitor (BCPI) from the hemolymph of Bombyx mori; alpha and beta are two forms differing only in ... Bombyx cysteine proteinase inhibitor. Subscribe to New Research on Bombyx cysteine proteinase inhibitor ...
Effects of Insect Viruses and Pesticides on Glutathione S-Transferase Activity and Gene Expression in Bombyx mori
The silkworm Bombyx mori is an important economic animal for silk production. However, it is liable to infection by a number of ... Effects of Insect Viruses and Pesticides on Glutathione S-Transferase Activity and Gene Expression in Bombyx mori. ... and Bombyx mori densonucleosis virus (BmDNV) and under the stress of pesticides (insecticide and herbicide). BmDNV induced an ... "Effects of Insect Viruses and Pesticides on Glutathione S-Transferase Activity and Gene Expression in Bombyx mori," Journal of ...
Characterization of the Gene BmEm4, a Homologue of Drosophila E(spl)m4, from the Silkworm, Bombyx mori
... Fenghui Zeng,1,2 ... We identified a homologue of the E(spl)m4 gene from Bombyx mori called BmEm4 and examined the expression patterns of BmEm4 mRNA ... Figure 1: Nucleotide and deduced amino acid sequence for Bombyx mori E(spl)m4 (BmEm4). Conserved GY-box (GTCTTCC), K-box ( ... a) Sequence comparisons of Bombyx mori E(spl)m4 (accession number FJ436408), Drosophila melanogaster E(spl)m4 (accession number ...
Homology of Dipteran Bristles and Lepidopteran Scales: Requirement for the Bombyx mori achaete-scute Homologue ASH2 | Genetics
Zhou, Q., Y. Li, X. Shen, Y. Yi, Y. Zhang et al., 2006 The scaleless wings mutant in Bombyx mori is associated with a lack of ... Zhou, Q., T. Zhang, W. Xu, L. Yu, Y. Yi et al., 2008 Analysis of four achaete-scute homologs in Bombyx mori reveals new ... Guo, T., J. Wang, X. Guo, S. Wang and C. Lu, 2005 Transient in vivo gene delivery to the silkworm Bombyx mori by EGT-null ... Chen, Y., B. Yao, Z. Zhu, Y. Yi, X. Lin et al., 2004 A constitutive super-enhancer: homologous region 3 of Bombyx mori ...
PLOS Genetics: Precocious Metamorphosis in the Juvenile Hormone-Deficient Mutant of the Silkworm, Bombyx mori
The meotic prophase in Bombyx mori females analyzed by three dimensional reconstructions of synaptonemal complexes
Bombyx mori. During leptotene and early zygotene, the lateral component … ... Bombyx mori. During leptotene and early zygotene, the lateral components become attached to the nuclear envelope at a specific ... The meotic prophase in Bombyx mori females analyzed by three dimensional reconstructions of synaptonemal complexes Chromosoma. ...
Ultrastructure, chromosomal karyotype, and molecular phylogeny of a new isolate of microsporidian Vairimorpha sp. BM ...
Toxins | Free Full-Text | Effect of Venom from the Jellyfish Nemopilema nomurai on the Silkworm Bombyx mori L.
The silkworm Bombyx mori L. (B. mori) has a significant impact on the economy by producing more than 80% of the globally ... Keywords: venom; jellyfish; Nemopilema nomurai; toxicity; Bombyx mori L. venom; jellyfish; Nemopilema nomurai; toxicity; Bombyx ... The silkworm Bombyx mori L. (B. mori) has a significant impact on the economy by producing more than 80% of the globally ... Yu H, Li R, Chen X, Yue Y, Xing R, Liu S, Li P. Effect of Venom from the Jellyfish Nemopilema nomurai on the Silkworm Bombyx ...
Bomb)yx-Mori - IAAC Blog
- Many other Lepidoptera produce cocoons, but only a few-the Bombycidae , in particular the genus Bombyx , and the Saturniidae , in particular the genus Antheraea -have been exploited for fabric production. (wikipedia.org)
- Bombyx is the genus of true silk moths or mulberry silk moths of the family Bombycidae, also known as silkworms, which are the larvae or caterpillars of silk moths. (wikipedia.org)
- Bombyx lemeepauli is a species of Bombycidae in the genus Bombyx. (wikipedia.org)
- The spore morphology, chromosomal karyotype, and molecular systematic of a new microsporidian which was isolated from the domesticated silkworm Bombyx mori (Lepidoptera: Bombycidae) in Shandong, China have been studied. (springer.com)
- Screening for nutritional trait change by feeding leaf spot diseased of mulberry leaf to silkworm, Bombyx mori L. (Lepidoptera: Bombycidae) is an essential prerequisite for better understanding of reduced food consumption, nutritional efficiency loss and low efficiency conversion. (omicsonline.org)
- Changes in the activity of digestive enzymes in response to chemical mutagen diethyl sulfate in the silkworm Bombyx mori L. (Lepidoptera: Bombycidae). (scialert.net)
- The silkworm Bombyx mori L. (Lepidoptera: Bombycidae) is a model research insect among Lepidoptera and of economically important in the production of silk worldwide (Tansil et al. (idmakeup.cz)
- Pdf of Materials fabrication from Bombyx mori silk fibroin, Nat Protoc. (hackteria.org)
- In this study, spermine was used to modify Bombyx mori silk fibroin (BSF) to synthesize cationized BSF (CBSF). (rsc.org)
- An in vitro silk fibroin production system has been developed by culture of posterior silk glands from Bombyx mori. (idmakeup.cz)
- Acknowledgements This work has benefited from financial support through the 2010 POSDRU/89/1.5/S/52432 project, The complete sequence of the Bombyx mori fibroin gene has been determined by means of combining a shotgun sequencing strategy with physical map-based sequencing procedures. (konjac-maigrir.com)
Hemolymph of Bombyx mori1
- In the present study, native ferritin was isolated from the hemolymph of Bombyx mori using native-polyacrylamide gel electrophoresis (native-PAGE) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). (ovid.com)
Cloned from the silkworm1
- A digestive beta-glucosidase cDNA was cloned from the silkworm, Bombyx mori. (unboundmedicine.com)
- Here, we describe the establishment of a targeted gene replacement system in Bombyx mori to express the major ampullate spidroin-1 gene ( MaSp1 ) from the spider Nephila clavipes . (pnas.org)
- We identified a homologue of the E(spl)m4 gene from Bombyx mori called BmEm4 and examined the expression patterns of BmEm4 mRNA and protein. (hindawi.com)
- Upregulation of Atg5 and AIF gene expression in synchronization with programmed cellular death events in integumental epithelium of Bombyx mori induced by a dipteran parasitoid infection. (degruyter.com)
- Using the YELLOW protein sequence in Drosophila melanogaster to BLAST silkworm EST database, we found a gene family composed of seven members with a conserved MRJP domain each and named it YELLOW protein family of Bombyx mori . (biomedcentral.com)
- Linkage and mapping analyses of the normal marking gene +P in the silkworm (Bombyx mori) using SSR markers. (semanticscholar.org)
- Fifteen clones obtained from a cDNA library of mock-infected cells and one from a library prepared at 2 h postinfection (p.i.) were found to comprise one distinct gene, which was identified as the Bombyx homolog (bLaminin) of Drosophila laminin β1. (deepdyve.com)
- Identification and Characterization of the Cyclin-Dependent Kinases Gene Family in Silkworm, Bombyx mori. (semanticscholar.org)
- It also attaches great significance to gene expression and species evolution, however, a systematic investigation towards the codon usage in Bombyx mori ( B. mori ) has not been reported yet. (biomedcentral.com)
- From beginning, molecular studies analysing tissue-specific gene expression and developmental profiling in moths such as the silkworm (Bombyx mori) and the tobacco budworm (Heliothis virescens) bring one of the more important points to discuss about the function of this protein family: CSP genes are expressed throughout the whole insect body in both internal and external tissues at various developmental stages [5 , 6] . (scidoc.org)
- A cDNA clone (Rab14) from Bombyx mori was expressed in Escherichia coli as a glutathione S-transferase fusion protein and purified. (pubmedcentralcanada.ca)
- Zha, X.-F. P-element Somatic Inhibitor Protein Binding a Target Sequence in dsx Pre-mRNA Conserved in Bombyx mori and Spodoptera litura . (mdpi.com)
- A Bombyx mori YELLOW protein family is found to be composed of at least seven members. (biomedcentral.com)
- Protein Banding Pattern and Major Amino Acid Component in De-Oiled Pupal Powder of Silkworm, Bombyx mori Linn. (scialert.net)
- Characterization of an entomopathogenic fungi target integument protein, Bombyx mori single domain von Willebrand factor type C, in the silkworm, Bombyx mori. (readbyqxmd.com)
- The ability to undertake cell division is resumed in the course of diapause termination caused by such a treatment as acclimation to 5 degrees C. As an initial trial to investigate the relationship between diapause and embryonic cell cycling, we have cloned and sequenced two Bombyx cDNAs encoding two distinct cdc2-related Ser/Thr protein kinases. (semanticscholar.org)
- The silkworm was domesticated from the wild silkmoth Bombyx mandarina , which has a range from northern India to northern China , Korea, Japan, and the far eastern regions of Russia . (wikipedia.org)
- cDNA and deduced amino acid sequences of apolipophorin-IIIs from Bombyx mori and Bombyx mandarina. (scienceexchange.com)
- The cDNA sequence for apolipophorin-III from two strains of Bombyx mori (N4 and P50) and the Japanese and Chinese strains of Bombyx mandarina were determined. (scienceexchange.com)
- Large scale full-length cDNA sequencing reveals a unique genomic landscape in a lepidopteran model insect, Bombyx mori. (nih.gov)
- In this study, full open reading frames of one beta and three alpha types of karyopherin were cloned from cDNA of the domestic silkworm (Bombyx mori). (readbyqxmd.com)
- Here, we report a cDNA designed BmIHoP encoding an IHop homolog cloned from the fat body of the silkworm, Bombyx mori , using conserved domain homology search method. (alliedacademies.org)
- However, the function and regulatory mechanism of Tip60 homolog in Bombyx mori are not elucidated. (konjac-maigrir.com)
- Sequence analysis of the BamHI F fragment of the genome of Bombyx mori nuclear polyhedrosis virus (BmNPV) revealed an open reading frame whose deduced amino acid sequence had homology to those of cysteine proteases of the papain superfamily. (nih.gov)
- Homologous regions (hrs) (hr1,hr2-left,hr2-right,hr3,hr4-left,hr 4-right, and hr5) similar to those found in the Autographa californica nuclear polyhedrosis virus (AcNPV) genome were found in the Bombyx mori NPV (BmNPV) genome. (asm.org)
- Zemskov, E. A. 2003-01-01 00:00:00 The Bombyx mori nucleopolyhedrovirus (BmNPV) contains five baculovirus repeated ORF ( bro ) genes, all of which are expressed as delayed early genes. (deepdyve.com)
- Notably, BomaNPV S2 exhibited a distinguishing feature in that its host range covered that of both Bombyx mori nucleopolyhedrosis virus (BmNPV) and Autographa californica multiple nucleopolyhedrovirus (AcMNPV) in cultured cells. (microbiologyresearch.org)
Larvae of silkworm1
- Phoxim (O,O-diethyl O-(alpha-cyanobenzylideneamino) phosphorothioate) is a powerful organophosphorus pesticide with high potential for Bombyx mori larvae of silkworm exposure. (nih.gov)
- We found that Bm-ASH2 , one of four Bombyx achaete-scute homologs, is highly expressed in early pupal wings of wild-type silkworms, but its expression is severely reduced in sl pupal wings. (genetics.org)
- Parasitization of silkworm, Bombyx mori by invasive larva of dipteran parasitoid Exorista bombycis caused upto 20% revenue loss in sericulture. (degruyter.com)
- who have some picture about the ovary of Bombyx mori larva. (biology-online.org)
- I dissected the Bombyx mori larva for get its ovary.but I can't confirm about the ovary's shape.some article say that it 's trigon.If you have ever done that.please give a dissection picture about that.thank you! (biology-online.org)
- Bombyx mori Parvo-like virus (China Zhenjiang isolate) had termed as BmDNV-Z.NS3 is one of the three nonstructural proteins that is responsible for the replication of virus DNA. (ccsenet.org)
- Functional Material Features of Bombyx mori Silk Light vs. For many decades, numerous at-tempts have been made to prevent and control Pheromones and other semiochemicals are transported in the insect antenna by odorant-binding proteins (OBPs), which ferry the signals across the sensillum lymph to the olfactory receptors (ORs). (konjac-maigrir.com)
- Identification and characterization of genes abnormally expressed in wing-deficient mutant (flügellos) of the silkworm, Bombyx mori. (nih.gov)
- Conservation Status of Silkworm (Bombyx mori) Genetic Resources in the World. (wikipedia.org)
- Lead your students through the life cycle of a silkworm, Bombyx mori . (carolina.com)
- Thangaraj P, Neelamegam RK, Nagarajan K, Muthukalingan K. Interaction of azadirachtin with the lipid-binding domain: Suppression of lipid transportation in the silkworm, Bombyx mori. (harvard.edu)
- An attempt has been made in this review article to briefly discuss the magnitude of correlation and heritability in selection strategies for the improvement of quantitative traits in desired direction in the silkworm, Bombyx mori (L. (scirp.org)
- Narasimharaju R., Govindan R., Ashoka J. and Rayar S.G. (1990) Genetic variability for quantitative traits in silkworm, Bombyx mori L. Entomon ,15, 197-201. (scirp.org)
- Petkov N. (1981a) The possibility of forecasting the effectiveness of selecting lines of the silkworm, Bombyx mori based on weight and silk content of cocoons. (scirp.org)
- Singh T., Chandrashekharaiah A. and Samson M.V. (1994) Selection strategies in relation to correlation and heritability in the silkworm, Bombyx mori (L). Bulletin of Sericultural Research, 5, 37- 41. (scirp.org)
- Sabhat A., Farooq M., Sofi A.M. and Malik M.A. (2009) Heritability, genetic advance and correlation analysis of some reproductive traits in silkworm, Bombyx mori L. Journal of Experimental Zoology, 12, 307-310. (scirp.org)
- Jayaswal K.P., Singh T. and Subba Rao G. (1991) Effect of female pupal weight on fecundity of mulberry silkworm Bombyx mori. (scirp.org)
- Gowda B.L.V., Sannaveerappanayar V.T. and Shivayogeshwar B. (1989) Fecundity and hatchability in mulberry silkworm, Bombyx mori (L) as influenced by pupal weight. (scirp.org)
- This virus infects crustaceans and insects, especially the silkworm (Bombyx mori). (sciencephoto.com)
- The silkworm Bombyx mori is an important economic animal for silk production. (bioone.org)
- Effect of Venom from the Jellyfish Nemopilema nomurai on the Silkworm Bombyx mori L. (mdpi.com)
- The silkworm Bombyx mori L. ( B. mori ) has a significant impact on the economy by producing more than 80% of the globally produced raw silk. (mdpi.com)
- Yu H, Li R, Chen X, Yue Y, Xing R, Liu S, Li P. Effect of Venom from the Jellyfish Nemopilema nomurai on the Silkworm Bombyx mori L.. Toxins . (mdpi.com)
- Role of Bmbuffy in hydroxycamptothecine-induced apoptosis in BmN-SWU1 cells of the silkworm, Bombyx mori. (sigmaaldrich.com)
- Studies on the Mode of Action of the Diapause Hormone in the Silkworm, BOMBYX MORI L. (biologists.org)
- Three structurally related and novel antibacterial peptides have been isolated from the haemolymph of the silkworm, Bombyx mori, immunized with Escherichia coli. (biochemj.org)
- The silkworm, Bombyx mori L. forms one of the very important insects of choice with large number of strains which is best exemplified for utilization of heterosis by crossing them in different combinations ( Datta and Nagaraju, 1987 ). (academicjournals.org)
- This study explored food allergy caused by eating silkworm (Bombyx mori L.) pupae, a traditionally accepted food and animal feed in East and Southeast Asia, and identified two new allergens by proteomic and immunological methods. (termedia.pl)
- In order to study the mechanism of programmed cell death (PCD) in silkworm Bombyx mori, we employed suppressive subtractive hybridization to screen associated genes. (ajol.info)
- Nutrigenic Efficiency Change and Cocoon Crop Loss due to Assimilation of Leaf Spot Diseased Mulberry Leaf in Silkworm, Bombyx mori L. (omicsonline.org)
- Sajad UH, Hasan SS, Dhar A, Vishal (2014) Nutrigenic Efficiency Change and Cocoon Crop Loss due to Assimilation of Leaf Spot Diseased Mulberry Leaf in Silkworm, Bombyx mori L. . J Plant Pathol Microbiol 5:220. (omicsonline.org)
- Computational identification and characteristics of novel microRNAs from the silkworm (Bombyx mori L. (semanticscholar.org)
- Studies on the posterior silk gland of the silkworm Bombyx mori. (rupress.org)
- The anti-juvenoid, SSP-11 ((E) 4-chloro-a, a, a-trifluro-N [ 1 (1 H-imidazole- 1 -y1)- 2-propoxy ethylyledene] O, toludine) was used to induce trimoulters from tetramoulter larvae of the silkworm, Bombyx mori (race NB7). (openarchives.gr)
- Alkaline and acid phosphatases of the silkworm, Bombyx mori L. (iisc.ernet.in)
- Sridhara, S and Bhat, JV (1963) Alkaline and acid phosphatases of the silkworm, Bombyx mori L. In: Journal of Insect Physiology, 9 (5). (iisc.ernet.in)
- The variations in the activities of the alkaline and acid phosphatases of the silkworm, Bombyx mori, were studied in all stages of the life cycle. (iisc.ernet.in)
- In the silkworm Bombyx mori, morphological variations associated with the E complex, an analogue of the Drosophila melanogaster bithorax complex, mainly determine the shape and number of prolegs on abdominal segments. (konjac-maigrir.com)
- All key components of the Hippo pathway, including the transcriptional coactivator Yorkie that is the most critical substrate and downstream effector of the Hippo kinase cassette, are found in the silkworm, Bombyx mori. (umm.ac.id)
- The Live Silkworm (bombyx mori) Educational Teacher Kit v2.0 includes 50 Silkworms, (2) 1/2 lb powdered silkworm food, one silkworm keeper, and one silkworm food grater. (westcoastsilkworms.com)
- Silkworm, or Bombyx-Mori, is a "second product" insect. (iaacblog.com)
- We established a genetic linkage map employing 518 simple sequence repeat (SSR, or microsatellite) markers for Bombyx mori (silkworm), the economically and culturally important lepidopteran insect, as part of an international genomics program. (semanticscholar.org)
- Bombyx mori cypovirus (BmCPV) is an important pathogen of silkworm (B. mori), the economically beneficial insect . (bvsalud.org)
- Adult: Adult (male), Unknown locality, June 14, 2009 (Photo by Paolo Mazzei) Enterobacter, pathogenicity, toxin complex, Bombyx mori INTRODUCTION A bacterial strain was isolated from flacherie-diseased silkworms in 1967 (Ono, Enomoto and Iwanami 1967 ) â ¦ It shows high degradation activity during the pre-pupa period, when the silk gland undergoes apoptosis and remodeling. (konjac-maigrir.com)
- Simple sequence repeat-based consensus linkage map of Bombyx mori. (semanticscholar.org)
- Interestingly, building a fast distance-based phylogenetic tree (RAxML) from a sequence alignment (MUSCLE) comparing Bombyx and Heliothis CSP amino acid full-sequences shows that moth CSPs clearly segregate into tissue-specific orthologous groups with strong bootstrap support (Figure 1). (scidoc.org)
- The four Bombyx sequences also showed significant similarity to the sequence of apolipophorin-III from another lepidopteran, Manduca sexta (83-84%), particularly in the five amphipathic alpha-helices that are proposed to play a critical role in the binding of apolipophorin-III to lipophorin. (scienceexchange.com)
- A neuropeptide (s) showing the same activity as a summer-morph-producing hormone of the Asian comma butterfly, P.c-aureum, was found to exist in brain-suboesophageal ganglion (Br-SG) extract of the silkmoths, Bombyx mori. (nii.ac.jp)
- The family contains a single species, Bombyx mori from the Greek for silkworm + mulberry tree (on which it feeds). (harvard.edu)
- Bombyx" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)
- Zhang J, Liu H, Sun Z, Xie J, Zhong G, Yi X. Azadirachtin induced apoptosis in the prothoracic gland in Bombyx mori and a pronounced Ca 2+ release effect in Sf9 cells. (ijbs.com)
- Identification of amino acid residues of Bacillus thuringiensis delta-endotoxin CryIAa associated with membrane binding and toxicity to Bombyx mori. (asm.org)
- The mRNA of Bombyx mori Bras2 has 1412 bp. (hindawi.com)
- A cysteine protease encoded by the baculovirus Bombyx mori nuclear polyhedrosis virus. (nih.gov)
- Transcriptomic analysis of developmental features of Bombyx mori wing disc during metamorphosis. (nih.gov)
- In this study, we used the Bombyx mori mutant scaleless ( sl ), which displays a nearly complete loss of wing scales, to explore the mechanism of lepidopteran wing-scale formation. (genetics.org)
- The dissolution conditions seem to be different between the domesticated Bombyx, and other wild strains. (hackteria.org)
- Bombyx mori is the domesticated silk worm bred for silk production. (hackteria.org)
- Degumming or removal of sericin - 5g/L sodium carbonate, pH, T, 1h (for Bombyx). (hackteria.org)