Milk: The white liquid secreted by the mammary glands. It contains proteins, sugar, lipids, vitamins, and minerals.Polysorbates: Sorbitan mono-9-octadecanoate poly(oxy-1,2-ethanediyl) derivatives; complex mixtures of polyoxyethylene ethers used as emulsifiers or dispersing agents in pharmaceuticals.Indicators and Reagents: Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499)Sensitivity and Specificity: Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)Enterochromaffin-like Cells: Neuroendocrine cells in the glands of the GASTRIC MUCOSA. They produce HISTAMINE and peptides such as CHROMOGRANINS. ECL cells respond to GASTRIN by releasing histamine which acts as a paracrine stimulator of the release of HYDROCHLORIC ACID from the GASTRIC PARIETAL CELLS.Milk, HumanUnited StatesSurface-Active Agents: Agents that modify interfacial tension of water; usually substances that have one lipophilic and one hydrophilic group in the molecule; includes soaps, detergents, emulsifiers, dispersing and wetting agents, and several groups of antiseptics.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Micropore Filters: A membrane or barrier with micrometer sized pores used for separation purification processes.Reagent Kits, Diagnostic: Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.Radioimmunoassay: Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.Filtration: A process of separating particulate matter from a fluid, such as air or a liquid, by passing the fluid carrier through a medium that will not pass the particulates. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Biochemistry: The study of the composition, chemical structures, and chemical reactions of living things.Protein Sorting Signals: Amino acid sequences found in transported proteins that selectively guide the distribution of the proteins to specific cellular compartments.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Transillumination: Passage of light through body tissues or cavities for examination of internal structures.X-Ray Film: A film base coated with an emulsion designed for use with x-rays.Projection: A defense mechanism, operating unconsciously, whereby that which is emotionally unacceptable in the self is rejected and attributed (projected) to others.Biological Science Disciplines: All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.Image Processing, Computer-Assisted: A technique of inputting two-dimensional images into a computer and then enhancing or analyzing the imagery into a form that is more useful to the human observer.Software: Sequential operating programs and data which instruct the functioning of a digital computer.Drug Industry: That segment of commercial enterprise devoted to the design, development, and manufacture of chemical products for use in the diagnosis and treatment of disease, disability, or other dysfunction, or to improve function.X-Ray Intensifying Screens: Screens which absorb the energy in the x-ray beam that has penetrated the patient and convert this energy into a light pattern which has as nearly as possible the same information as the original x-ray beam. The more light a screen produces for a given input of x-radiation, the less x-ray exposure and thus shorter exposure time are needed to expose the film. In most film-screen systems, the film is sandwiched between two screens in a cassette so that the emulsion on each side is exposed to the light from its contiguous screen.Equipment Design: Methods of creating machines and devices.Thermodilution: Measurement of blood flow based on induction at one point of the circulation of a known change in the intravascular heat content of flowing blood and detection of the resultant change in temperature at a point downstream.Exosomes: A type of extracellular vesicle, containing RNA and proteins, that is secreted into the extracellular space by EXOCYTOSIS when MULTIVESICULAR BODIES fuse with the PLASMA MEMBRANE.Cell-Derived Microparticles: Extracellular vesicles generated by the shedding of CELL MEMBRANE blebs.Secretory Vesicles: Vesicles derived from the GOLGI APPARATUS containing material to be released at the cell surface.Transport Vesicles: Vesicles that are involved in shuttling cargo from the interior of the cell to the cell surface, from the cell surface to the interior, across the cell or around the cell to various locations.Echinostomiasis: Infection by flukes of the genus Echinostoma.Verrucomicrobia: A phylum of gram-negative bacteria containing seven class-level groups from a wide variety of environments. Most members are chemoheterotrophs.Extracellular Space: Interstitial space between cells, occupied by INTERSTITIAL FLUID as well as amorphous and fibrous substances. For organisms with a CELL WALL, the extracellular space includes everything outside of the CELL MEMBRANE including the PERIPLASM and the cell wall.Cell Communication: Any of several ways in which living cells of an organism communicate with one another, whether by direct contact between cells or by means of chemical signals carried by neurotransmitter substances, hormones, and cyclic AMP.Paracoccidioides: A mitosporic fungal genus. P. brasiliensis (previously Blastomyces brasiliensis) is the etiologic agent of PARACOCCIDIOIDOMYCOSIS.Cryptococcus neoformans: A species of the fungus CRYPTOCOCCUS. Its teleomorph is Filobasidiella neoformans.Sarcocystis: A genus of protozoa found in reptiles, birds, and mammals, including humans. This heteroxenous parasite produces muscle cysts in intermediate hosts such as domestic herbivores (cattle, sheep, pigs) and rodents. Final hosts are predators such as dogs, cats, and man.Ink Blot Tests: Projective tests utilizing ink blots to which a subject responds. They are used in personality diagnosis.Sarcocystosis: Infection of the striated muscle of mammals by parasites of the genus SARCOCYSTIS. Disease symptoms such as vomiting, diarrhea, muscle weakness, and paralysis are produced by sarcocystin, a toxin produced by the organism.Divorce: Legal dissolution of an officially recognized marriage relationship.Marriage: The social institution involving legal and/or religious sanction whereby individuals are joined together.Blotting, Western: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.Spouses: Married persons, i.e., husbands and wives, or partners. Domestic partners, or spousal equivalents, are two adults who have chosen to share their lives in an intimate and committed relationship, reside together, and share a mutual obligation of support for the basic necessities of life.Health Status: The level of health of the individual, group, or population as subjectively assessed by the individual or by more objective measures.Herpes Simplex: A group of acute infections caused by herpes simplex virus type 1 or type 2 that is characterized by the development of one or more small fluid-filled vesicles with a raised erythematous base on the skin or mucous membrane. It occurs as a primary infection or recurs due to a reactivation of a latent infection. (Dorland, 27th ed.)Reproductive Health: The physical condition of human reproductive systems.Yersinia: A genus of gram-negative, facultatively anaerobic rod- to coccobacillus-shaped bacteria that occurs in a broad spectrum of habitats.Laboratories: Facilities equipped to carry out investigative procedures.Yersinia enterocolitica: A species of the genus YERSINIA, isolated from both man and animal. It is a frequent cause of bacterial gastroenteritis in children.Yersinia Infections: Infections with bacteria of the genus YERSINIA.Diagnostic Techniques and Procedures: Methods, procedures, and tests performed to diagnose disease, disordered function, or disability.Menu PlanningYersinia pestis: The etiologic agent of PLAGUE in man, rats, ground squirrels, and other rodents.Plasticizers: Materials incorporated mechanically in plastics (usually PVC) to increase flexibility, workability or distensibility; due to the non-chemical inclusion, plasticizers leach out from the plastic and are found in body fluids and the general environment.Yersinia pseudotuberculosis: A human and animal pathogen causing mesenteric lymphadenitis, diarrhea, and bacteremia.Laboratory Animal Science: The science and technology dealing with the procurement, breeding, care, health, and selection of animals used in biomedical research and testing.

High-throughput screening of small molecules in miniaturized mammalian cell-based assays involving post-translational modifications. (1/58879)

BACKGROUND: Fully adapting a forward genetic approach to mammalian systems requires efficient methods to alter systematically gene products without prior knowledge of gene sequences, while allowing for the subsequent characterization of these alterations. Ideally, these methods would also allow function to be altered in a temporally controlled manner. RESULTS: We report the development of a miniaturized cell-based assay format that enables a genetic-like approach to understanding cellular pathways in mammalian systems using small molecules, rather than mutations, as the source of gene-product alterations. This whole-cell immunodetection assay can sensitively detect changes in specific cellular macromolecules in high-density arrays of mammalian cells. Furthermore, it is compatible with screening large numbers of small molecules in nanoliter to microliter culture volumes. We refer to this assay format as a 'cytoblot', and demonstrate the use of cytoblotting to monitor biosynthetic processes such as DNA synthesis, and post-translational processes such as acetylation and phosphorylation. Finally, we demonstrate the applicability of these assays to natural-product screening through the identification of marine sponge extracts exhibiting genotype-specific inhibition of 5-bromodeoxyuridine incorporation and suppression of the anti-proliferative effect of rapamycin. CONCLUSIONS: We show that cytoblots can be used for high-throughput screening of small molecules in cell-based assays. Together with small-molecule libraries, the cytoblot assay can be used to perform chemical genetic screens analogous to those used in classical genetics and thus should be applicable to understanding a wide variety of cellular processes, especially those involving post-transitional modifications.  (+info)

Retinoic acid, but not arsenic trioxide, degrades the PLZF/RARalpha fusion protein, without inducing terminal differentiation or apoptosis, in a RA-therapy resistant t(11;17)(q23;q21) APL patient. (2/58879)

Primary blasts of a t(11;17)(q23;q21) acute promyelocytic leukaemia (APL) patient were analysed with respect to retinoic acid (RA) and arsenic trioxide (As2O3) sensitivity as well as PLZF/RARalpha status. Although RA induced partial monocytic differentiation ex vivo, but not in vivo, As203 failed to induce apoptosis in culture, contrasting with t(15;17) APL and arguing against the clinical use of As203 in t(11;17)(q23;q21) APL. Prior to cell culture, PLZF/RARalpha was found to exactly co-localize with PML onto PML nuclear bodies. However upon cell culture, it quickly shifted towards microspeckles, its localization found in transfection experiments. Arsenic trioxide, known to induce aggregation of PML nuclear bodies, left the microspeckled PLZF/RARalpha localization completely unaffected. RA treatment led to PLZF/RARalpha degradation. However, this complete PLZF/RARalpha degradation was not accompanied by differentiation or apoptosis, which could suggest a contribution of the reciprocal RARalpha/PLZF fusion product in leukaemogenesis or the existence of irreversible changes induced by the chimera.  (+info)

Complete nucleotide sequence of the 27-kilobase virulence related locus (vrl) of Dichelobacter nodosus: evidence for extrachromosomal origin. (3/58879)

The vrl locus is preferentially associated with virulent isolates of the ovine footrot pathogen, Dichelobacter nodosus. The complete nucleotide sequence of this 27.1-kb region has now been determined. The data reveal that the locus has a G+C content much higher than the rest of the D. nodosus chromosome and contains 22 open reading frames (ORFs) encoding products including a putative adenine-specific methylase, two potential DEAH ATP-dependent helicases, and two products with sequence similarity to a bacteriophage resistance system. These ORFs are all in the same orientation, and most are either overlapping or separated by only a few nucleotides, suggesting that they comprise an operon and are translationally coupled. Expression vector studies have led to the identification of proteins that correspond to many of these ORFs. These data, in combination with evidence of insertion of vrl into the 3' end of an ssrA gene, are consistent with the hypothesis that the vrl locus was derived from the insertion of a bacteriophage or plasmid into the D. nodosus genome.  (+info)

Relationship between UDP-glucose 4-epimerase activity and oligoglucose glycoforms in two strains of Neisseria meningitidis. (4/58879)

Sodium dodecyl sulfate-polyacrylamide gel analysis of lipooligosaccharide (LOS) from Neisseria meningitidis has demonstrated considerable microheterogeneity in the variable region of LOS due to the presence of novel glycoforms. As a step toward understanding the basis for the expression of these novel glycoforms, we have examined the LOS structures and UDP-glucose 4-epimerase (epimerase) activity levels in two strains (NMB and MA-1) and their respective galE mutants. Strain NMB was found to have low epimerase activity and to contain multiple glycoforms, some of which appear to contain only glucose sugars. The galE mutant had only the oligoglucose glycoforms. Strain MA-1 had higher epimerase activity at both log and stationary phases (2- and 12.5-fold, respectively) and one glycoform with a putative lactosyl structure. Strain MA-1 galE had two glycoforms that contained one or two glucose residues. To understand the molecular basis for the different epimerase activities, we examined the predicted amino acid sequences of the respective galE open reading frames and determined the relative amounts of GalE protein. We found no significant differences between the predicted amino acid sequence of the GalE protein in NMB and that in MA-1. We observed no significant differences in the level of GalE protein between MA-1 and NMB at exponential or stationary phase. We also observed an 8.2-fold drop in epimerase activity in NMB between the log and stationary phases that was not due to the GalE protein level or low glucose levels.  (+info)

Characterization of human bactericidal antibodies to Bordetella pertussis. (5/58879)

The Bordetella pertussis BrkA protein protects against the bactericidal activity of complement and antibody; however, some individuals mount an immune response that overcomes this bacterial defense. To further characterize this process, the bactericidal activities of sera from 13 adults with different modes of exposure to B. pertussis (infected as adults, occupational exposure, immunized with an acellular vaccine, or no identified exposure) against a wild-type strain and a BrkA complement-sensitive mutant were evaluated. All of the sera killed the BrkA mutant, suggesting past exposure to B. pertussis or cross-reactive organisms. Several samples had no or minimal activity against the wild type. All of the sera collected from the infected and occupationally exposed individuals but not all of the sera from vaccinated individuals had bactericidal activity against the wild-type strain, suggesting that some types of exposure can induce an immune response that can overcome the BrkA resistance mechanism. Adsorbing serum with the wild-type strain removed the bactericidal antibodies; however, adsorbing the serum with a lipopolysaccharide (LPS) mutant or an avirulent (bvg mutant) strain did not always result in loss of bactericidal activity, suggesting that antibodies to either LPS or bvg-regulated proteins could be bactericidal. All the samples, including those that lacked bactericidal activity, contained antibodies that recognized the LPS of B. pertussis. Bactericidal activity correlated best with the presence of the immunoglobulin G3 (IgG3) antibodies to LPS, the IgG subtype that is most effective at fixing complement.  (+info)

Characterization of Moraxella (Branhamella) catarrhalis lbpB, lbpA, and lactoferrin receptor orf3 isogenic mutants. (6/58879)

Pathogenic members of the family Neisseriaceae produce specific receptors to acquire iron from their host's lactoferrin and transferrin. Recently, putative Moraxella catarrhalis lactoferrin receptor genes and a third open reading frame (lbpB, lbpA, and orf3) were cloned and sequenced. We describe the preliminary characterization of isogenic mutants deficient in LbpB, LbpA, or Orf3 protein.  (+info)

Mechanisms related to [18F]fluorodeoxyglucose uptake of human colon cancers transplanted in nude mice. (7/58879)

[18F]Fluorodeoxyglucose ([18F]FDG), a glucose analogue, has been widely used for tumor imaging. To investigate the mechanisms related to [18F]FDG uptake by tumors, an experiment involving nude mice was performed. METHODS: Human colon cancer cell lines SNU-C2A, SNU-C4 and SNU-C5 were transplanted to nude mice. Using immunohistochemical staining and Western blot, the expression of glucose transporter (Glut) isoforms (Glut-1 through -5) in xenografted tumors was analyzed. For the analysis of messenger ribonucleic acid (mRNA) expression, reverse-transcription polymerase chain reaction and Northern blot were used and the enzyme activity of hexokinase in cancer tissues was measured by continuous spectrophotometric rate determination. RESULTS: [18F]FDG uptake in SNU-C4 and SNU-C5 cells was higher than in normal colon cells. Among these cells and xenografted tumors, SNU-C5 showed the highest level of [18F]FDG uptake, followed by SNU-C4 and SNU-C2A. An immunostaining experiment showed intense staining of Glut-1 in SNU-C5 tumors but somewhat faint staining in SNU-C4. SNU-C5 tumors also showed positive staining with Glut-3, although this was not the case with SNU-C2A and SNU-C4. Western blot analysis showed the expression of Glut-1 and Glut-3 in all tumors. Experiments involving Northern blot analysis and reverse-transcription polymerase chain reaction confirmed the overexpression of Glut-1 mRNA in all tumors, with the highest level in SNU-C5. The level of Glut-3 mRNA was also elevated in SNU-C5 tumors but not in SNU-C2A and SNU-C4. The enzyme activity of hexokinase did not vary among different tumors. CONCLUSION: Gluts, especially Glut-1, are responsible for [18F]FDG uptake in a nude mouse model of colon cancer rather than hexokinase activity. Increased numbers of glucose transporters at the plasma membrane of cancer cells is attributed to an increased level of transcripts of glucose transporter genes and may be a cause of increased [18F]FDG uptake, at least in colon cancer tumors.  (+info)

Cloning of the peroxiredoxin gene family in rats and characterization of the fourth member. (8/58879)

Peroxiredoxin (PRx) exhibits thioredoxin-dependent peroxidase activity and constitutes a family of proteins. Four members of genes from rat tissues were isolated by PCR using degenerated primers based on the sequences which encode a pair of highly conserved Cys-containing domains, and were then cloned to full-length cDNAs. These included two genes which have previously been isolated in rats, PRx I and PRx II, and two rat homologues of PRx III and PRx IV. We showed, for the first time, the simultaneous expression of all four genes in various rat tissues by Northern blotting. Since a discrepancy exists regarding cellular distribution, we further characterized PRx IV by expressing it in COS-1 cells. This clearly demonstrates that PRx IV is a secretory form and functions within the extracellular space.  (+info)

*Far-western blotting

... blotting' step. In a western blot, specific proteins are then identified using an antibody probe. Far-western blotting employs ... Far-western blotting is a molecular biological method which is based on the technique of western blotting to detect protein- ... Whereas western blotting uses an antibody to detect a protein of interest, far-western blotting uses a non-antibody protein, ... Thus, whereas western blotting is used for the detection of certain proteins, far-western blotting is rather employed to detect ...

*Antibody

Kurien B, Scofield R (2006). "Western blotting". Methods. 38 (4): 283-293. doi:10.1016/j.ymeth.2005.11.007. PMID 16483794. ... in Western blot analyses to identify proteins separated by electrophoresis, and in immunohistochemistry or immunofluorescence ... Western blot, immunodiffusion, immunoelectrophoresis, and magnetic immunoassay. Antibodies raised against human chorionic ... Monoclonal antibody Neutralizing antibody Secondary antibodies Single-domain antibody Slope spectroscopy Western blot ...

*Molecular biology

Using western blotting techniques allows not only detection but also quantitative analysis. Analogous methods to western ... In western blotting, proteins are first separated by size, in a thin gel sandwiched between two glass plates in a technique ... The terms northern, western and eastern blotting are derived from what initially was a molecular biology joke that played on ... Kurien, Biji T.; Scofield, R. Hal (1 April 2006). "Western blotting". Methods (San Diego, Calif.). 38 (4): 283-293. doi:10.1016 ...

*Lysis buffer

... western blot). Most lysis buffers contain salts (e.g. Tris-HCl or EDTA) to regulate the acidity and osmolarity of the lysate. ... "Sample preparation for western blot , Abcam". www.abcam.com. Retrieved 2016-03-16. https://www.thermofisher.com/order/catalog/ ...

*Image Studio Lite

"Western Blot Quantification with LI-COR Image Studio Lite". How to WESTERN-BLOT. Retrieved 7 March 2014. "Densitometry Tutorial ... "Quantification of Western Blots Using ImageJ". How to WESTERN-BLOT. Retrieved 7 March 2014. "Image Studio Lite Software". licor ... The primary function of Image Studio Lite is to quantitate relative abundance of proteins on a Western blot or DNA/RNA in an ... Image Studio™ Lite is free image processing software used for quantitation of Western blot images and images from related ...

*Amido black 10B

Western blot normalization "AMIDO BLACK 10B". chemicalland21.com. Retrieved December 2, 2017. MSDS at Oxford University. ... such as the western blot. It is also used in criminal investigations to detect blood present with latent fingerprints. It ... 10B is an amino acid staining azo dye used in biochemical research to stain for total protein on transferred membrane blots, ...

*MChip

"ELISA/Western blot tests for HIV". Medline Plus. Retrieved 2011-08-16. ...

*Transcription factor

The sample is detected on a western blot. By using electrophoretic mobility shift assay (EMSA), the activation profile of ...

*Equine protozoal myeloencephalitis

Diagnosis of equine protozoal myeloencephalitis: Western blot analysis. Proc Am Coll Vet Intern Med Forum 587-590, 1993. ... A review: Determining the sensitivity and specificity of western blot tests for diagnosis of Equine protozoal myeloencephalitis ...

*Electroblotting

Alternatively the proteins may react with ligands like lectins giving rise to the term affinity blotting. Western blotting SDS- ... 141-. ISBN 978-81-7993-379-4. United State Patent 4840714 Protein (Western) Blotting - Introduction to Antibodies Membrane ... Following electrophoresis, a standard tank or semi-dry blotting transfer system is set up. A stack is put together in the ... Because the proteins may retain or regain part of their structure during blotting they may react with specific antibodies ...

*Thermal shift assay

Just as for FastPP the Western blot could be replaced by staining for a polyhistidine-tag in the SDS-PAGE gel. Additionally, ... Optionally, for instance for proteins expressed at low levels, a Western blot is then run to determine at what temperature a ... The presence of the target protein in each soluble fraction is determined by Western blotting and used to construct a CESTA ... To reduce the workload, Western Blots could be replaced by SDS-PAGE gel polyhistidine-tag staining, provided that the protein ...

*Rocker (laboratory)

Retrieved on 31 Jan 2012 Western Blot Protocol. University of Idaho, College of Agricultural and Life Sciences. Retrieved on 31 ... Rockers are commonly used for staining and de-staining gels after electrophoresis, hybridization, washing, blotting, Cell ...

*Lyme disease

ELISA screening and Western blot confirming); or 3) single-tier IgG (old infection) Western blot. Previously, the CDC only ... Studies show the Western blot IgM has a specificity of 94-96% for people with clinical symptoms of early Lyme disease. The ... Western blot, ELISA, and PCR can be performed by either blood test via venipuncture or cerebrospinal fluid (CSF) via lumbar ... The case definition now includes the use of Western blot without prior ELISA screen. The number of reported cases of the ...

*Coomassie Brilliant Blue

Coomassie Blue staining can also be used as a loading control staining method in western blot analysis. It is applied as an ... Welinder, Charlotte; Ekblad, Lars (2011-03-04). "Coomassie Staining as Loading Control in Western Blot Analysis". Journal of ...

*Epicocconone

... making it a powerful loading control for Western blots. In addition to the natural variant from the fungus, there are several ... A powerful loading control for Western blots". Proteomics. 14 (2-3): 162-8. doi:10.1002/pmic.201300089. PMID 24339236. Peixoto ...

*Ponceau S

... sequencing of blotted polypeptides and is therefore one method of choice for locating polypeptides on Western blots for blot- ... Coomassie Brilliant Blue Western blot normalization "Ponceau S solution". Sigma-Aldrich. Retrieved 2016-11-24. Al-Amoudi, M. S ... Western blotting), as well as on cellulose acetate membranes. A Ponceau S stain is useful because it does not appear to have a ...

*Meir Wilchek

Examples include western blot, ELISA, ELISPOT and pull-down assays. More recently, Wilchek participated in structural studies ...

*Calcitriol receptor

Signal requirements and characterization by western blots and DNA sequencing". J. Biol. Chem. 266 (12): 7588-95. PMID 1850412. ...

*Multiplex (assay)

Ambroz K., (2006). "Improving quantitation accuracy for western blots".Image Analysis 09/2006.[1] Laxman B, Morris DS, Yu J, et ... for specific activities Multiplex detection enables the simultaneous detection of two or more targets on a western blot. ...

*Phycobilisome

... versus cyanine dye detection capabilities in Western blot application. Light Harvesting by Phycobilisomes Annual ... Western blotting and protein microarrays. Some phycobilisomes have an absorption and emission profile similar to Cy5, they can ...

*SH2D3A

The protein may be useful in Western Blots and ELISAs. SH2D3A has been shown to interact with the epidermal growth factor ...

*Chemical specificity

Another technique that relies on chemical specificity is Western blotting, which is utilized to detect a certain protein of ... "An overview of technical considerations for Western blotting applications to physiological research". Scandinavian Journal of ...

*Myc-tag

Examples are cellular localization studies by immunofluorescence or detection by Western blotting. The peptide sequence of the ...

*Eastern blot

Far-western blotting Far-eastern blotting Glycosylation Phosphorylation Northwestern blot Western blot Northern blot Southern ... Since this is essentially a western blot, the charge reversal was used to dub this method an eastern blot. (2005) Eastern blot ... distinguishing them from a standard far-western blot. In principle, eastern blotting is similar to lectin blotting (i.e. ... 1996) Eastern-western blot was first used by Bogdanov et al. The method involved blotting of phospholipids on PVDF or ...

*Fast parallel proteolysis

Reaction products can be separated by SDS-PAGE or western blot. The protease thermolysin can be fully inactivated by EDTA. This ...

*John Steuart Newbigging

He is buried in the family plot in the western extension of Greyfriars Kirkyard. He was brother to Patrick Newbigging. In 1840 ... bl&ots=qiSB2S-AAr&sig=3VaQ2oHFJfZFIf4D3Pb2SGfqe5s&hl=en&sa=X&ved=0ahUKEwjghq_73pjXAhXEKFAKHTZnDsYQ6AEILzAD#v=onepage&q=j%20s% ...
98.88%--99.49%. In May 2008, fewer false-positive tests occurred; in that month, five (0.11% of 4,749 oral fluid tests) were found to be false positive (specificity: 99.89%). During this second instance of increasing numbers of false-positive oral fluid tests, the clinics continued offering immediate follow-up finger-stick whole-blood rapid tests for all patients with reactive oral fluid tests. The usefulness of the NYC DOHMH policy was affirmed by the strong correlation between results from whole-blood rapid tests and confirmatory Western blot tests. During December 2005--May 2008, 1,720 patients had reactive oral fluid rapid tests, and definitive Western blot results were recorded for 1,664 (Figure 2). Missing Western blot results (24 patients) and inconclusive Western blot results (32 patients) were excluded from additional analysis. Of these 1,664 patients, 1,194 also provided a finger-stick specimen; 850 (71.2%) had a reactive finger-stick test, of whom 840 (98.8%) were positive by Western ...
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Im comparing the expression of a protein in my treated animals and controls by western blot. So the way I do it is that I scan the bands ( target protein and actin), measure the color intensity in each band, then put it in this equation; target/actin in treated animals divided by target/actin in control animals. I think in this way I can have a good understanding of the net increase in my target protein. Does it make sense? Do you know any documented equation for that purpose?. ...
OATP1B3 is a 12 transmembrane domain protein expressed at the basolateral membrane of human hepatocytes where it mediates the uptake of numerous drugs and endogenous compounds. Previous western blot results suggest the formation of OATP1B3 multimers. In order to better understand the function of OATP1B3 under normal physiological conditions, we investigated its oligomerization status. We transiently transfected OATP1B3 with a C-terminal His-, FLAG- or HA-tag in HEK293 cells and used co-immunoprecipitation and a Proximity Ligation Assay to detect interactions between the different constructs. All three constructs retained similar transport rates as wild-type OATP1B3. Immunofluorescence experiments indicated that in contrast to wild-type, His- and FLAG-tagged OATP1B3, where the C-terminal end is on the cytoplasmic side of the membrane, the C-terminal end of HA-tagged OATP1B3 is extracellular. After cross-linking, anti-FLAG antibodies were able to pull down FLAG-tagged OATP1B3 (positive control) ...
Abstract: Purpose: CD133 (prominin-1), a 5-transmembrane glycoprotein, has recently been considered an important marker that represents the subset population of cancer stem-like cells. The purpose of the present study is to isolate cancerous stem-like cells from normal healthy volunteers and prostate cancer patients (CD133+) which also express MDR1 and to ascertain the influence of Oct-4 on stem-ness and differentiation of these CD133+ cells towards epithelium. Methods: CD133+ cells were isolated using magnetic beads from normal healthy volunteers and prostate cancer patients (NV-CD133+and PC-CD133+). The isolated cells were analyzed using flow cytometry and Western blot technique for CD133, MDR1 and Oct-4. CD133+MDR1+ cells were cultured in presence and absence of antihuman Oct-4 blocking antibody. Results: PC-CD133+ cells displayed higher Oct-4 expression with the ability to self-renew and may represent a reservoir with differentiation potential for generating prostate cancer cells. ...
This technical article describes a standard western-blotting procedure using the commercially available NuPAGE electrophoresis Mini-Gel ...
Western blot analysis of protein A production. Analysis of protein A production by Western Blot in hVISA/VISA and VSSA pairs. A. Pair 1 (lane 1, JKD 6000; lane
This presentation will cover the three most critical failures common to Western blot experiments and provide solutions on how you can avoid these failure to protect your protein.
stripping of Western blot? - posted in SDS-PAGE and Western Blotting: hi could anyone let me know stripping of western blot work?? thank a lot regards
Depletion of xWRN reduces SSA. (A) Western blot of the depleted NPE. The three lanes on the right are quantitation controls and contain normal NPE at 1%, 2%, an
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Watch this interview with LI-COR to learn about the Odyssey® CLx, Odyssey® Fc and Odyssey® Sa imaging systems and new features for quantitative western blots. AACR 2012.
Am 10.11.2007, 19:44 Uhr, schrieb ,mitne from usp.br,: , does anyone know what protein could be used as a control when looking , for SERUM PROTEINS on Western Blot? If you tell us what you want to control for, it would be easier to answer the question. Is serum albumin as a control for loading + transfer efficiency not sufficient ...
For example, the dose of 267 essential to accomplish 50% suppres sion of P AKT when utilized alone was cal culated to get 30M, though in blend with Dt the dose required to achieve the identical FA was reduced three fold. In contrast, the densitometry information indicated that for LCC6Her2 cells, the concentration of 267 necessary in blend with Dt to achieve a defined effect on P AKT inhibition was substantially greater than that essential when 267 was used being a single agent. For instance, 30M 267 was essential to achieve an FA of 0. 5 when 267 was applied alone, how ever, during the presence of Dt the concentration of 267 expected to attain an FA of 0. 5 was estimated to be 130M. Differences within the blend results due to Her2 above expression were confirmed working with the MCF 7 and MCF 7Her2 cell lines, as summarized within the representative western blots shown in Figure 5.. Qualitative assessments on the P AKT western blot data are presented selleck chemical checkpoint inhibitors as ...
This video explains why striping, reprobing and cutting of Western blots is wasteful and unreliable as it results in uneven removal of proteins, erroneous cutting and poor reproducibility. The video goes onto describe how stain-free technology from Bio-Rad allows for total protein normalization and prevents the need for striping, reprobing and cutting.
The immunoblot test is one of the most common research techniques and our immunoblots include many loaded samples. Order your immunoblots from ProSci today!
Define Western blot: a blot consisting of a sheet of nitrocellulose or nylon that contains spots of protein for identification by a suitable…
Nowoczesna diagnostyka laboratoryjna. Dwuetapowa diagnostyka boreliozy. Molekularna diagnostyka alergii. Testy na alergię z krwi. Przeciwciała onkoneuronalne. IIFT. ELISA. Blot.
Nowoczesna diagnostyka laboratoryjna. Dwuetapowa diagnostyka boreliozy. Molekularna diagnostyka alergii. Testy na alergię z krwi. Przeciwciała onkoneuronalne. IIFT. ELISA. Blot.
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What is Dot Blot: similar technique as western blotting for detecting proteins in samples that are spotted through circular templates directly onto the membrane.. General Methods & Techniques. Antigen-Antibody Specific Applications. Virological Applications. Tumor, Disease & Diagnostic Applications. Dot Blot Definition - A technique for detecting, analyzing, and identifying proteins, similar to the Western blot technique but differing in that protein samples are not separated electrophoretically but are spotted through circular templates directly onto the membrane or paper substrate (source: NDI Foundation).. Protocols, troubleshooting and tips for successful dot blotting - Dot blot is a technique for detecting and identifying proteins, similar to the Western blot technique but differing in that protein samples are not separated electrophoretically but are spotted through circular templates directly onto the membrane or paper substrate. Antigens may be applied directly to nitocellulose membrane ...
CYTIP (cytohesin interacting protein) is an intracellular molecule induced in dendritic cells during maturation. CYTIP modulates the binding intensity of the adhesion molecule LFA-1. If dendritic cells are silenced for CYTIP they keep longer contacts with T-cells resulting in a lower T cell stimulation. We identified Suppressor of cytokine signaling-1 (SOCS-1) as a binding partner for CYTIP in human monocyte derived dendritic cells. In Western blot analyses we found that CYTIP expression is down regulated at later time points, starting at about 72 hours after induction of maturation. To investigate a possible role for SOCS-1 in taking CYTIP to the degradation machinery of the cell we measured endogenous CYTIP protein levels in mature dendritic cells transfected with SOCS-1 encoding plasmid in quantitative Western blot analyses. We observed lower amounts of endogenous CYTIP in mature dendritic cells transfected with SOCS-1 encoding plasmid compared with untransfected dendritic cells. Experiments with the
Looking for online definition of Western blot analysis in the Medical Dictionary? Western blot analysis explanation free. What is Western blot analysis? Meaning of Western blot analysis medical term. What does Western blot analysis mean?
According to the CDC, serologic assays that use a second enzyme immunoassay are acceptable alternatives to western immunoblot assays.
Western blotting is one of the most powerful methods in molecular biology for identifying and characterizing specific proteins in complex protein mixtures. We have now streamlined western-blotting procedures so that the entire analysis can be performed during a single 3-hour, or two 2-hour laboratory sessions. In exercise 801, students use the procedure outlined below to identify albumin, transferrin and gamma globulins in serum and then to study the evolutionary relationships of albumin in vertebrates.. ...
Western blotting is one of the most powerful methods in molecular biology for identifying and characterizing specific proteins in complex protein mixtures. We have now streamlined western-blotting procedures so that the entire analysis can be performed during a single 3-hour, or two 2-hour laboratory sessions. In exercise 801, students use the procedure outlined below to identify albumin, transferrin and gamma globulins in serum and then to study the evolutionary relationships of albumin in vertebrates.. ...
Western blot, a method to separate proteins by size and analyse their relative expression levels, is a much maligned technique of molecular cell biology. The website PubPeer is flooded with evidence of manipulated Western blots, where gel lanes were inappropriately spliced, or where bands digitally duplicated or erased. Some even question the technology as such,…
Western blot, a method to separate proteins by size and analyse their relative expression levels, is a much maligned technique of molecular cell biology. The website PubPeer is flooded with evidence of manipulated Western blots, where gel lanes were inappropriately spliced, or where bands digitally duplicated or erased. Some even question the technology as such,…
Northern blotting (inglise northern blot, northern blot technique) on meetod RNA osade identifitseerimiseks vastavates proovides, kus agaroosgeelis lahku viidud RNA lõigud viiakse üle nt nitrotselluloospaberile ja üleviimise tulemusel saab RNA lõike uurida nt radioaktiivselt markeeritud geeniproovi kaudu[1].. ...
Hi All Im trying to detect two small proteins of 2.5 and 4.5 kDa by western analysis but Im having a little problem resolving such small proteins on 15% and 20% PAGE. Anyone have any suggestions ...
One of the last steps to optimize is how long to expose a blot before developing it. Modern technologies have attempted to supplant this step: new chemiluminescent detectors have reduced our need to measure how long to expose our blots. However, for those of us that cant afford this fancy new equipment, we may rely on the good old film and developer. Using this methodology, the amount of time film is exposed to your completed blot will affect how dark your bands are, as well as how much background you. Of course, you want to optimize this step so that your image is clear and not misleading. Overexposing a blot can lead to bands that all look the same; in reality, they might not be if you were to expose your blot less. Additionally, taking several exposures - at both short and long lengths - will give you a range from which to choose ...
Densitometry data generated for Western blots are commonly used to compare protein abundance between samples. In the last decade, it has become apparent that assumptions underpinning these comparisons are often violated in studies reporting Western blot data in the literature. These violations can lead to erroneous interpretations of data and may contribute to poor reproducibility of research. We assessed the reliability of Western blot data obtained to study human myometrial tissue proteins. We ran dilution series of protein lysates to explore the linearity of densitometry data. Proteins analysed included |i|α|/i|SMA, HSP27, ERK1/2, and GAPDH. While ideal densitometry data are directly proportional to protein abundance, our data confirm that densitometry data often deviate from this ideal, in which case they can fit nonproportional linear or hyperbolic mathematical models and can reach saturation. Nonlinear densitometry data were observed when Western blots were detected using infrared
These data demonstrate icIL-1ra type I mRNA, sIL-1ra mRNA, and IL-1ra protein in human coronary arteries. IL-1ra protein colocalizes with IL-1β predominantly in the endothelium of these arteries, with less IL-1ra mRNA expression occurring in less-diseased DCM arteries. To our knowledge, this is the first documentation of IL-1ra expression by HCAECs. We also show only icIL-1ra type I mRNA expression in HUVECs and HCAECs stimulated in vitro with LPS/PMA or TGF-β. Previous reports have failed to detect IL-1ra mRNA in these cell types with the use of other stimuli and less sensitive detection methods, such as Northern blots,5 and there are no previous reports of immunohistochemistry for IL-1ra in human vessels. With the use of immunoprecipitation/Western blot techniques, our data also confirm that the expression of IL-1ra in HUVECs and HCAECs is intracellular. We have also demonstrated that the rare allele (IL-1RN *2/*2) of a VNTR polymorphism in the IL-1RN gene is associated with significantly ...
Hi, Yes, its possible your Western Blot (WB) test was a false-positive, particularly if this was your only HIV test! WB tests should only be run as a confirmatory test after someone has a...
Many cancer patients develop tumor-reactive immune responses against antigens that are either expressed on the surface of tumor cells or released from them into the peripheral circulation. In this study, tumor-reactive immunoglobulins, present in the sera of ovarian cancer patients, were used to identify commonly recognized tumor-associated antigens on ovarian tumor cells. Western immunoblot analysis of cellular proteins, obtained from UL-1 ovarian tumor cell line, demonstrated several commonly recognized immunoreactive proteins. Two of these proteins (Mr 32,000 and 71,000) were selected for further investigation. Cellular proteins isolated from normal human ovarian epithelia, in a similar fashion, failed to exhibit corresponding immunoreactivity to these proteins. As an additional control, sera from normal (nontumor-bearing) individuals failed to identify these proteins on Western immunoblots. Furthermore, the absorption of the ovarian cancer patients sera with normal ovarian epithelial tissue ...
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Antigen-Antibody Pen For Hamster Primary antibodies Western Blot Marking Pen PEN-T8 Western blot annotations, antigen-antibody pens, ECl, Western, dot blots Antigen-Antibody Pen For Hamster Primary antibodies Western Blot Marking Pen PEN-T8 Western blot annotations, antigen-antibody pens, ECl, Western, dot blots
1.Grow cells to mid-log (~1x107 cells/ml; A600 = 0.7 or klett 80) and collect 1.5 ml cells in 1.5 ml microfuge tube (1 minute, 14000xg). It is important not to grow the cells to a high density as this method will not work well ...
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OUTLINE: This is an open-label, pilot study.. Patients receive oral erlotinib hydrochloride once daily on days 1-28. Treatment repeats every 28 days for at least 2 courses in the absence of disease progression or unacceptable toxicity.. Patients undergo tumor fine-needle aspiration biopsies under ultrasound or CT scan guidance at baseline and between days 12-15 for laboratory studies. Laboratory studies include quantitative western blot and enzyme-linked immunosorbent assays, gene mutation and amplification, and ex vivo assays. Tumor cells are also analyzed for changes in phosphorylation status and/or expression levels of pharmacodynamic markers, including total- and phospho-epidermal growth factor receptor, total- and phospho-ERK, and total- and phospho-AKT.. PROJECTED ACCRUAL: A total of 40 patients will be accrued for this study. ...
The human body louse, Pediculus humanus, showed eighteen midgut proteins ranging between 12 and 117 kDa, when analysed by SDS-PAGE electrophoresis. Seven of them (12 kDa, 17 kDa, 29 kDa, 35 kDa, 40 kDa, 55 kDa and 97 kDa) were major bands based on their intensity of staining. The immunization of rabbits with a midgut extract elicited the production of protective polyclonal antibodies. These antibodies reacted strongly with all major midgut proteins as well as with 63 kDa and 117 kDa proteins when tested by the Western blot technique. The analysis of the proteins revealed that the 12 kDa, 25 kDa, 29 kDa, 35 kDa, 45 kDa, 87 kDa and 97 kDa proteins are glycosylated and none of them contained a lipid moiety. By electroelution, the proteins of 35 kDa and 63 kDa were purified. On trypsinization, the proteins of 35 kDa and 63 kDa produced four major fragments (F1, F2, F3, and F4) when resolved on a 18% SDS-PAGE. The F1 fragment of the 35 kDa protein reacted with the polyclonal antibodies by the ...
OBJECTIVE: Dual vector AAV systems are being utilised to enable gene therapy for disorders in which the disease gene is too large to fit into a single capsid. Fragmented adeno-associated viral (fAAV) vectors containing single inverted terminal repeat truncated transgenes have been considered as one such gene replacement strategy. Here we aim to add to the current understanding of the molecular mechanisms employed by fAAV dual vector systems. METHODS: Oversized (|8kb) transgene constructs containingABCA4coding sequence were packaged as truncated fragments |5kb in size into various AAV serotypes.In vitrotransductions with these fAAV vector preparations were conducted with mRNA and protein expression products assessed by way of RT-PCR, qPCR and western blot techniques. RESULTS: Transductions with fAAV vector preparations yieldedABCA4mRNA, but did not generate detectable levels of protein. Sequencing of the transcript population revealed the presence of full lengthABCA4CDS with additional
A polyclonal antiserum was prepared against a purified microsomal chitinase isolated from the fungus Choanephora cucurbitarum. Indirect immunofluorescence was used to localize chitinase at various developmental stages of five zygomycetous fungi and during abiotrophic mycoparasite interaction with a susceptible and resistant host. This was compared to localization of oligomers of N-acetylglucosamine with the lectin wheat germ agglutinin (WGA). Dotimmunoblot and Western blot techniques revealed that the anti-serum reacted strongly with the antigen from which it was derived. Cross reactivity of the antiserum was found with WGA and another chitin binding lectin, Phyto/acca americana agglutinin (PAA). Immuno-fluorescence results showed the direct involvement of chitinase in spore swelling, germination, sporangium development and response during mechanical injury. There appeared to be no involvement of chitinase during apical hyphal growth or new branch initiation in any of the fungi tested despite ...
Abstract: Rheumatoid arthritis is a systemic inflammatory disease with still unknown aetiology. The purpose of our study was to comparatively investigate the level of haemoglobin, as well as of the heat shock proteins HSP60 and HSP70 as well as their specific antibodies serum levels in rheumatic patients, in order to evaluate their potential role as an aid in the diagnosis of this chronic pathology. This study was performed on patients with rheumatoid polyarthritis. The haemoglobin plasmatic concentration was assessed by a quantitative determination using the automatic analyzer Beckman Coulter Act5diff, while the thermal shock proteins HSP 60, HSP 70 as well as their respective serum antibodies were determined by Western blot method and ELISA assay, respectively. All the patients involved in this study exhibited low values of haemoglobin, known to be an important marker of haematological disorders. These results maintain the idea that anaemia, heart disease, osteoporosis are the most frequent ...
VisiBlot Standard I is a mixture of 10 recombinant proteins of a molecular weight range from 25 kDa to 150 kDa. Protein bands of 25 kDa, 45 kDa and 85 kDa are prestained allowing monitoring of protein separation during SDS PAGE. The remaining five proteins contain several IgG binding sites. Hence marker proteins bind to primary or secondary antibodies used in Western Blotting facilitating easy marker visualization on the transfer membrane. Because the proteins have no chromophore attached, the marker enables accurate molecular weight estimation. Recommended loading volume for a mini gel is 5 µl/lane. Ready-to-use, no reconstitution, further dilution or heating required Prestained bands for monitoring electrophoresis and membrane transfer Visualization of marker proteins on Western Blots by horseradish peroxidase or alkaline phosphatase-based immune-detection methods Molecular weight determination of proteins detected on transfer membrane
... : Effects of 50 μM DHA, EPA, SA, and PA or 25 μM OA and LA on IL-2-induced extracellular signal-regulated kinase (ERK) 1/2 phosphorylation. Lymphocytes were incubated with 5 μg/mL ConA for 24 h. Afterwards, lymphocytes were washed with PBS and cultured with the different fatty acids in the presence or absence of IL-2 (30 ng/mL) for 1 h. Total proteins were extracted from lymphocytes for western-blotting analysis. Blots were analyzed by densitometry and the results normalized to their respective controls, which were set to a value of 100% for each experiment. The values are presented as the means ± SEM. ###p,0.001 for comparison with the control in the absence IL-2); *p,0.05, **p,0.01, and ***p,0.001 for comparison with the control treated with IL-2 ...
... TPO negatively, EPO or SCF. shipped WT, but not really mutant Lnk Ur8 obstructed TPO-induced M-MOK megakaryoblastic leukemic cell growth. In comparison, WT Lnk Ur8 demonstrated no development inhibitive impact on non-hematopoietic HELA or COS-7 cell. Furthermore, we showed that TPO-induced M-MOK cell development inhibition by WT Lnk Ur8 was dose-dependent. Penetrated WT Lnk 3rd theres r8 activated cell cycle apoptosis and detain. Immunoprecipitation and Traditional western blots data indicated WT Lnk Ur8 interacted with endogeneous Jak2 and downregulated Jak-Stat and MAPK phosphorylation level in M-MOK cells after TPO enjoyment. Treatment with particular inhibitors (TG101348 and PD98059) indicated Jak-Stat and MAPK paths had been essential for TPO-induced growth of M-MOK cells. Further studies using TF-1 and HEL leukemic cell-lines demonstrated that WT Lnk Ur8 inhibited Jak2-reliant cell growth. Using cable ...
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BioAssay record AID 690837 submitted by ChEMBL: Suppression of STAT3 mediated Mcl-1 expression in human MDA-MB-468 cells by immunoblotting assay.
Dont see the protein expression by Western blotting even after trying multiple antibodies and other protocol optimizations? The reason could be as simple as the protein loss from the membrane. Use our PVDF western blot troubleshooting strategy for greater success.. ...
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Buenas noches, Un ELISA positivo sin un examen confirmatorio positivo, tal como un Western Blot positivo, no es concluyente. Si te entiendo correctamente, ahora tienes dos ELISAS positivos y dos...
I went through a divorce in Fall of 2011 and had learned that my spouse was quite sexually active outside the marriage. I wanted to get verification of my sexual health status before beginning to date,...
Itation combined with mass spectrometry and western blot analysis. The results provide Vitamin D2 chemical information strong evidence showing that ASPM plays a
[97 Pages Report] Check for Discount on United States Western Blot imagers (Western Blot Analyzer) Market Report 2017 report by QYResearch Group. In this report, the United States Western Blot Imagers (Western...
A: CST does not routinely test antibodies for fluoresecent western blotting, though many work well in this application. Performing a fluorescent western blot using CST™ antibodies requires a modified immunoblotting protocol. We find that omitting Tween® 20 detergent from the blocking step (using only 5% nonfat dry milk in TBS) and drying the membrane prior to imaging are the only necessary changes from our standard protocol. Full details can be found in our Fluorescent Western Immunoblotting Protocol (Primary Ab Incubation In BSA) and our Fluorescent Western Immunoblotting Protocol (Primary Ab Incubation In Milk). Additionally, our Prestained Protein Marker, Broad Range (Premixed Format) #7720 serves well as a marker in fluorescent western blotting because it is autofluorescent at near-infrared wavelengths.. ...
Antigen-Antibody Pen For Rat Primary antibodies Western Blot Marking Pen PEN-R10 Antigen-Antibody Pen For Rat Primary antibodies Western Blot Marking Pen PEN-R10
BioAssay record AID 685115 submitted by ChEMBL: Induction of caspase-3 cleavage in human HuH7 cells at 2.5 to 5 uM incubated for 6 to 24 hrs by Western blot.
Western blots can be ugly. I mean down-right, horrifically, wall-of-shame ugly. Not only can they be embarrassing to show to your colleagues, but the ugliness can obscure your results, making it impossible to interpret your data.. Blotting consists of many experimental steps, which makes the technique naturally error-prone. Although standardized protocols exist, many fail to point out the small details that could turn a really ugly Western blot into a thing of beauty.. To save you the humiliation of presenting an ugly blot at lab meeting, I am sharing my 10 tips to good-looking Westerns.. ...
The western blot (alternatively, immunoblot) is an analytical technique used to detect specific proteins in a given sample of tissue homogenate or extract. It uses gel electrophoresis to separate...
doing western blot, I can not detect a specific protein (I use this cell line as my negative control), but when I do immunocytochemistry, that cell line shows a great amount of that protein! I am sure about western blot. what can I do regarding imunocytochemistry to get the same results as W.B ...
OriGene offers the most comprehensive source for human proteins and lysates, including purified proteins and corresponding MS standard, over expression lysates, cancer cell lysates and knockout cell lysates.
Adriano Aguzzi, a professor of neuropathology and director of the Swiss National Creutzfeldt-Jakob Disease Surveillance Center in Zurich, Switzerland, said in an e-mail that IHC can be as sensitive as the Western blot method "in certain specific instances." Aguzzis institution, however, routinely runs a Western blot test enhanced with another step "on every suspect case, because of its unrivalled specificity and because in most cases we find it to be much more sensitive than immunohistochemistry.". In the months since the November announcement, the USDA came under intense pressure from organizations such as Consumers Union for failing to conduct a Western blot test on the suspect cow.. Activists at Consumers Union pointed to reports in veterinary literature which suggested IHC could miss some cases.. Michael Hansen, a senior research associate with Consumers Union, says he confronted USDA officials about the issue during the June 6 meeting of Institute of Medicine of the National ...
In order to understand more thoroughly the antibody response against M. suis, we sought to characterize M. suis-specific antigens in blood-derived preparations of the agent. For this, we modified our recently published protocol for gaining M. suis DNA by a density gradient centrifugation step that was described to efficiently separate hemotrophic mycoplasmas from erythrocytic membranes (1). The results of our Western blot studies show that M. suis expresses in vivo at least eight specific proteins, ranging from 33 to 83 kDa in size, which are recognized by convalescent-phase sera. To the best of our knowledge, this is the first analysis of M. suis-specific antigens. Due to the use of host-derived M. suis antigens, the antibody response measured is expected to exclusively target antigens expressed by M. suis within the porcine host. However, it is impossible at this time to conceive any precise function for these proteins.. Of particular note is the consistent detection of three protein bands ...
In the present study, we instilled capsaicin into the uteruses of female rats and found that this procedure induces cross-organ sensitization on the pelvic-urethra reflex activity as well as the physiological target of this reflex, the contraction wave of the urethra, with a parallel increase in the phosphorylated NMDA NR2B subunit level in the spinal dorsal horn. Moreover, pharmacological blockage of the TRPV1 receptor attenuates the reflex potentiation caused by uterine capsaicin instillation as well as the increment in the phosphorylated NR2B subunit level, indicating that TRPV1 plays a role in the spinal NR2B subunit-dependent cross-organ sensitization induced by uterine capsaicin instillation.. Pelvic pain syndrome, one of the most frequent disorders in women, can lead to years of suffering, disability, and numerous unsuccessful medical treatments. Pelvic pain can arise from several sources, such as the gastrointestinal, urinary, genital, muscular, and skeletal systems. The ...
An examination of the ratios of the 16 repetitively spotted flavonoid 3 hydroxylase cDNAs using a t test showed that the mean ratio of the repeated cDNAs on replicate 1 (2.424) were statistically significant at a P value of 0.0001 when compared to an expected mean of a 2.0, or a two-fold expression difference. The low P values were also found for replicate 2 and for the mean value (3.245) of both replicates. Thus, the flavonoid 3 hydroxylase cDNAs are statistically significant outliers in the microarray analysis.. The microarray data presented here and showing that the cytoplasmic levels of the flavonoid 3 hydroxylase are higher in the T/T line agree very well with RNA blot data which showed that the flavonoid 3 hydroxylase gene has reduced expression in the seed coats of the t*/t* isoline compared to the T/T lines [13]. In addition to the RNA blot data showing differences in these mutant lines, we have definitively shown that the flavonoid 3 hydroxylase is encoded by the T locus by ...
Introduction Neospora caninum is an important cause of abortion in cattle worldwide. Dogs (feral and ... been isolated from swine, domestic or wild.
Caveolin-1 (Cav-1), a family of ubiquitously expressed oligomeric structural proteins in many mammalian cells, has been shown to be an effective regulator of tumorigenesis. Recent studies have indicated that Cav-1 can promote resistance to chemotherapy in a variety of tumors. However, the regulation of Cav-1 on chemoresistance in ovarian cancer is still unknown. In the present study, the mRNA and protein expression level was investigated by RT-PCR and western blot analysis, respectively, and the 50% inhibitory concentration (IC50) value was measured by MTT assay. The protein expression level of P-glycoprotein (P-gp), Notch-1, p-Akt and p-NF-κB p65 were detected using western blot analysis and the apoptotic ratio was determined using the Annexin V-FITC/PI detection kit. The results showed that the mRNA and protein expression levels of Cav-1 were significantly higher in SKOV3/DDP and A2780/DDP than in SKOV3 and A2780, respectively. Knockdown of Cav-1 significantly decreased the IC50 value in ...
You are provided with a sample of blood from a patient who is suspected of having Lymes disease (Borrelia). If you also have access to Borrelia cell surface proteins and goat anti-human IgG conjugated horse radish peroxidase.
Another essential attribute is the drug to antibody ratio (DAR). It can be derived from the drug distribution assay but can also be measured at the reduced or subunit level. This yields additional insights about linker hydrolysis or drug degradation. The maXis II can carry out these measurements with isotopic resolution enabling sensitive and confident detection of such degradation products. This same assay is also helpful in the research phase in order to compare the developability of various scaffolds or linker chemistries ...
Western blot on valguanalüüsi meetod, mida kasutatakse spetsiifiliste valkude tuvastamiseks. Analüüsi proovid on tavaliselt puhastamata ja sisaldavad polüpeptiide, mis takistab spetsiifilise valgu tuvastamist. Western blot analüüsil eraldatakse valguproovid geelelektroforeesiga, seejärel kantakse valgud üle nitrotselluloosmembraanile ning antikeha tuvastab uuritava valgu. Ensüümina kasutatakse HRP (horseradish peroxidase) või AP (alkaline phosphatase), mis on kovalentselt seotud antikehaga[1]. Valguanalüüsi käigus reageerib ensüüm kemoluminestsentse substraadiga ning tekib luminestsentsust ehk helendust mõõtev signaalisait valgu ja antikeha vahel.. Termini western blot võttis esimesena kasutusele W. Neal Burnette, meetod töötati välja George Starki laboris Stanfordis. Western blot nimetus pärineb sellest, et 1975. aastal avastas Edwin Southern DNA eraldamise meetodi, milles DNA kantakse agroosgeelilt nitrotselluloosmembraanile. Meetodit hakati avastaja järgi nimetama ...
When and how often laboratory tests are done may depend on many factors. The timing of laboratory tests may rely on the results or completion of other tests, procedures, or treatments. Lab tests may be performed immediately in an emergency, or tests may be delayed as a condition is treated or monitored. A test may be suggested or become necessary when certain signs or symptoms appear. Due to changes in the way your body naturally functions through the course of a day, lab tests may need to be performed at a certain time of day. If you have prepared for a test by changing your food or fluid intake, lab tests may be timed in accordance with those changes. Timing of tests may be based on increased and decreased levels of medications, drugs or other substances in the body. The age or gender of the person being tested may affect when and how often a lab test is required. Chronic or progressive conditions may need ongoing monitoring through the use of lab tests. Conditions that worsen and improve may ...
Zyagen Equine Tissue Premade Western Blot [EW-MT-1] - Equine tissue Western blots are made of high quality total proteins extracted from freshly harvested 19 different horse tissues (Panel# 1). Protein sample are subjected to rigorous quality control procedures. Blots can be used to: Quickly assess specific protein expression across different tissues. Determine size and relative abundance of protein in different tissues. Quality control: Every step
The effects against tumors exerted by marine active compounds have been highlighted and investigated. mitochondrial depolarization. Western blot analysis showed that HA nanoparticles-aggregated HET further increased mitochondrial-associated, caspase-dependent and caspase-independent, as well as endoplasmic reticulum stress-related factors in comparison with ...
AKL 30305113470 30/01/2013 MP Diagnostics HIV BLOT 2.2 Western Blot Assay MP BIOMEDICAL ASIA PACIFIC PTE., LTD., SINGAPORE PT. DIASTIKA
Words you can make out of immunohistochemistry. Anagrams of immunohistochemistry. Words made after you unscramble immunohistochemistry.
Садржај Уводник Резимеи Summaries and contents НОВА СРПСКА ПолитичкА МИСАО Нова едиција, vol. XIX (2011), no. 1-2 САДРЖАЈ Уводник. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Храм Вознесения Господня, построенный в XIX веке, никогда не закрывался и в годы гонений на церковь был единственным прибежищем для верующих из окрестных сел. Главной достопримечательностью этого храма является икона современного письма, находящаяся на отдельном аналое справа ближе к алтарю. На ней изображена простая женщина в обычной одежде - блаженная Мария (Матукасова), известная как Мария Самарская. В этом храме она несла свой молитвенный подвиг. В 80-е-90-е годы минувшего века многие самарцы, прослышав о прозорливой старице, приезжали к ней с ...
Methods In three primary RPE cell cultures (from three donor eyes) and in the human RPE cell line ARPE-19, FcRn and beta-2-microglobulin (β2M) mRNA levels were determined by real-time quantitative PCR. FcRn protein expression was analysed by western blot studies. Stimulation experiments were performed with recombinant human tumour necrosis factor (TNF)-α and interferon (IFN)-γ. HT-29, THP-1 and HeLa cell lines were used as FcRn positive and negative non-ocular controls, respectively. ...
ABSTRACT: BACKGROUND: Recent studies have confirmed the presence of viable Chlamydia in the bronchoalveolar lavage (BAL) fluid of pediatric patients with airway hyperresponsiveness. While specific IgG and IgM responses to C. pneumoniae are well described, the response and potential contribution of Ag-specific IgE are not known. The current study sought to determine if infection with Chlamydia triggers the production of pathogen-specific IgE in children with chronic respiratory diseases which might contribute to inflammation and pathology. METHODS: We obtained BAL fluid and serum from pediatric respiratory disease patients who were generally unresponsive to corticosteroid treatment as well as sera from age-matched control patients who saw their doctor for wellness checkups. Chlamydia-specific IgE was isolated from BAL and serum samples and their specificity determined by Western blot techniques. The presence of Chlamydia was confirmed by species-specific PCR and BAL culture assays. RESULTS: ...
A group of 41 peptides, each 24 amino acids long and overlapping with each other by 12 residues spanning the total gag open reading frame (orf) of HIV-1 (HTLV-IIIBH 10 isolate) were synthesized using Fmoc chemistry. The purified compounds were used in ELISA assays and tested for antibody reactivities in sera of human HIV-1-infected and noninfected individuals. Sera of HIV- humans showed reactivity against four defined regions, two in p17, one in p24, and one in p15. The values of these reactivities were elevated especially in serum samples of HIV- individuals showing cross-reaction with gag proteins on Western blot. Amino acid sequence comparison of HIV-1 gag proteins with those of human endogenous retroviruses (ERV K10, ERV 3) revealed significant similarities predominantly in the domains showing elevated antibody cross-reactions. The majority of sera from HIV-1+ individuals showed strong reactivities to the cross-reactive regions and to various other peptide sequences, a sequential epitope ...
Previous analyses suggested that some gO isoforms remain bound to gH/gL in the virion envelope and that others behave more like chaperones to promote assembly of unbound gH/gL dimers into the virion envelope (29). The chaperone model was based largely on the failure to detect gO in TR virions by reducing Western blot assay. Here, we used nonreducing gel systems to compare several strains of HCMV. In all cases, gO was clearly detected as part of a disulfide-linked complex with gH/gL in the virion envelope.. The discrepancy between the results with nonreducing and reducing Western blot assays is puzzling. As noted, antipeptide antibodies are generally thought to react better with proteins that have been separated under denaturing-reducing conditions because continuous epitopes should be more accessible. It is notable that virion-associated ADgO was clearly detected in the reducing Western blot experiments reported in Ryckman et al. (29) but not in the similar experiments reported here. This ...
Frataxin is the protein that is down-regulated in Friedreich ataxia (FA). There are currently no approved treatments for FA, although experimental approaches involving up-regulation or replacement of mature frataxin protein through numerous approaches are being tested. Many of the pre-clinical studies of these experimental approaches are conducted in mouse and monkey models as well as in human cell lines. Consequently, well-validated antibodies are required for use in western blot analysis to determine whether levels of various forms of frataxin have been increased. This group examined the specificity of five commercially available anti-frataxin antibodies and determined whether they detect mature frataxin in mouse heart tissue. Four protein standards of monkey, human, and mouse frataxin as well as mouse heart tissue were examined using polyacrylamide gel electrophoresis (PAGE) in combination with western blot analysis. One antibody failed to detect any of the frataxin standards or endogenous ...
Frataxin is the protein that is down-regulated in Friedreich ataxia (FA). There are currently no approved treatments for FA, although experimental approaches involving up-regulation or replacement of mature frataxin protein through numerous approaches are being tested. Many of the pre-clinical studies of these experimental approaches are conducted in mouse and monkey models as well as in human cell lines. Consequently, well-validated antibodies are required for use in western blot analysis to determine whether levels of various forms of frataxin have been increased. This group examined the specificity of five commercially available anti-frataxin antibodies and determined whether they detect mature frataxin in mouse heart tissue. Four protein standards of monkey, human, and mouse frataxin as well as mouse heart tissue were examined using polyacrylamide gel electrophoresis (PAGE) in combination with western blot analysis. One antibody failed to detect any of the frataxin standards or endogenous ...
Rabbit polyclonal antibody raised against a full-length human SERPINA5 protein. SERPINA5 (NP_000615.2, 1 a.a. ~ 406 a.a) full-length human protein. (H00005104-D01P) - Products - Abnova
Rabbit polyclonal antibody raised against a full-length human P4HTM protein. P4HTM (NP_808807.2, 1 a.a. ~ 563 a.a) full-length human protein. (H00054681-D01P) - Products - Abnova
Cell culture, transfection, immunocytochemistry, and Western blot. Calcium phosphate transfections, immunocytochemistry, and culture of dissociated hippocampal CA3/CA1 pyramidal neurons were performed as described previously (Wu et al., 2001). Enhanced green fluorescent protein (EGFP) was cotransfected with hemagglutinin (HA)- or myc-tagged constructs to visualize the detailed cell morphology. Hippocampal slice culture was done from 7-8 d postnatal rats and transfected biolistically at 2 d in vitro (DIV) as described previously (Lo et al., 1994). A modified procedure was used for Western blot experiments using cells growing on 12 mm coverslips. In brief, coverslips were placed in 1.5 ml microcentrifuge tubes containing 40 μl of 9 m urea and were crushed, and, after vigorous vortexing, samples were centrifuged (18,000 × g for 2 min). The protein concentration was determined by BCA assay (Pierce, Rockford, IL), and volumes were adjusted with 9 m urea so that all samples were 0.3-1.0 μg/μl. ...
The Alpha SureFire® Ultra™ HV Multiplex p-Akt 1/2/3 (Ser473) + Total Akt 1 assay kit is used to measure both the phosphorylation (Ser473) and total levels of endogenous Akt in cellular lysates. The kit measures phosphorylation on Ser473 of Akt 1/2/3, and total levels of Akt 1 only. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure Akt (Ser473) activation by either recombinant or endogenous receptors, and can be applied to primary cells.
Diagenode has developed a revolutionary new antibody for western blot that specifically reacts with this blue dye, enabling direct visualization of the different marker fragments on the blot.This makes the positioning of the marker and thus the accurate
Zyagen : Cross-Species Western Blot - Antibodies Kits, Reagents & Biochemical Tissue Sections RNA Products cDNA Products Genomic DNA Products Tissue Lysates Products Premade Northern Blots Premade Western Blots Recombinant Proteins & Peptides Tissue Microarrays
Visit CellSignal.com to view our WB & IP Reagents materials including Western Blot Reagents & more. CST - Customer satisfaction is our highest priority.
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Buy our Synphilin 1 293T transfected lysate (positive control). ab94102 has been validated in western blot. Abcam now offers a 12-month guarantee.
We previously described the polymorphism in the interferon regulatory factor-1 (IRF-1) gene as a novel correlate of resistance to HIV-1 infection in a Kenyan female sex worker cohort. However, the underlying mechanisms likely mediating this association remained to be elucidated. The initiation of HIV-1 long terminal repeat (LTR) transcription in peripheral blood mononuclear cells (PBMCs) from subjects with different IRF-1 haplotypes, representing protective, intermediate and the least protective IRF-1 allele combinations, were investigated here. A single-cycle pseudovirus construct expressing vesicular stomatitis virus envelop G-protein (VSV-G) and having an HIV-1 pNL4.3 backbone with luciferase insert was used to infect PBMCs with different IRF-1 haplotypes. The efficiency of early HIV-1 LTR transcription was monitored using a luciferase assay. IRF-1 protein levels induced by the infection were measured by quantitative Western blot. Our results showed that PBMCs with the protective IRF-1 ...
Abstract Polymorphism at codon 219 lysine in prion protein (PrP) is considered to affect the clinicopathological features of prion diseases including Creutzfeldt-Jakob disease (CJD) and to have an inhibiting effect on the pathogenesis of these diseases. We describe the first autopsied case of dura mater graft-associated CJD (dCJD) with heterozygosity of lysine at codon 219 in PrP observed in a Japanese subject. Although this case demonstrated the non-plaque type of dCJD and MM1 subgroup of CJD pathologically and biochemically, the patient demonstrated a long incubation period (19.3years), atypical periodic sharp-wave complexes with a dominant rhythm on EEG, partially scattered small deposits of plaque-like PrP along with synaptic type deposits of PrP on immunohistochemistry and an atypical MM1 glycosylation pattern with a relatively increased diglycosylated isoform of proteinase-resistant PrP on western blot analysis (i.e. "MM1 variant" pattern). These findings in this case were atypical of the ...
This study investigated the cancer inhibitory potential of a proanthocyanidin rich cranberry extract utilizing a panel of authenticated human esophageal adenocarcinoma cell lines (EAC), a nude mouse xenograft model, and the rat esophagogastrodoudenal anastomosis (EGDA) model of EAC. PACs effects on cell morphology, global gene expression, phase of cell cycle, and cell death induction via apoptosis, autophagy, and necrosis were evaluated. Methods included RapidDiff and MDC staining, Annexin/PI and BrdU staining with flow cytometric evaluation, transmission electron microscopy (TEM), protein evaluation utilizing standard Western blot techniques, and validation of global gene expression changes by real-time PCR. PAC treatment [50-100µg/ml] of EAC cancer cell lines resulted in significant increases in apoptosis, autophagy and necrosis. Differential cell death induction was noted based upon the cell lines resistance to a bile/acid cocktail mimicking acid exposure in humans as well as the status of ...

Comparison of a serum indirect fluorescent antibody test with two Western blot tests for the diagnosis of equine protozoal...Comparison of a serum indirect fluorescent antibody test with two Western blot tests for the diagnosis of equine protozoal...

A serum indirect fluorescent antibody test (IFAT) was compared with a Western blot (WB) and a modified Western blot (mWB) for ... was compared with a Western blot (WB) and a modified Western blot (mWB) for diagnosis of equine protozoal myeloencephalitis ( ... Comparison of a serum indirect fluorescent antibody test with two Western blot tests for the diagnosis of equine protozoal ... Comparison of a serum indirect fluorescent antibody test with two Western blot tests for the diagnosis of equine protozoal ...
more infohttp://www.islandscholar.ca/islandora/object/ir:2650

western blotwestern blot

... is a procedure to identify and quantify proteins. A mixture of protein is first submitted to an electrophoresis in ... Western blot is a procedure to identify and quantify proteins. A mixture of protein is first submitted to an electrophoresis in ...
more infohttps://www.ebi.ac.uk/ols/ontologies/mi/terms?obo_id=MI%3A0113

Blot Western - Wikipedia bahasa Indonesia, ensiklopedia bebasBlot Western - Wikipedia bahasa Indonesia, ensiklopedia bebas

Blot Western adalah sebuah metode untuk mendeteksi protein pada sampel jaringan. Imunoblot menggunakan elektroforesis gel untuk ... Western blot analysis of sub-cellular fractionated samples using the Odyssey Infrared Imaging System (a protocol) ... kiatsehat2010.blogspot.co.id/2010/12/test-untuk-aids-elisa-dan-western-blot.html ... blogku-251294.blogspot.co.id/2015/04/test-elisa-atau-western-blot_23.html ...
more infohttps://id.wikipedia.org/wiki/Blot_Western

western blottingwestern blotting

... Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu Fri Mar 23 18:15:24 EST 2001 *Previous message: ... I am doing western blotting now. The primary antibody ,I got is serum and I have no idea how many times I ,should dilute it ... Try titering it yourself on western strips of the same sample. Nick -- Nick Theodorakis nicholas_theodorakis at urmc. ... Previous message: western blotting *Next message: western blotting * Messages sorted by: [ date ] [ thread ] [ subject ] [ ...
more infohttp://www.bio.net/bionet/mm/methods/2001-March/088093.html

monoclonal antibody western blotmonoclonal antibody western blot

Western blot assay carried out using monospecific antibodies produced in the supernatant of a cell line obtained by fusing a ... Western blot assay carried out using monospecific antibodies produced in the supernatant of a cell line obtained by fusing a ...
more infohttps://www.ebi.ac.uk/ols/ontologies/mi/terms?obo_id=MI%3A0072

11 Ultimate Home Remedies for Mouth and Gum Blisters11 Ultimate Home Remedies for Mouth and Gum Blisters

Caused by different reasons such as infection, nutritional deficiency, psychological trauma etc., mouth blisters are hardly bigger than pencil dots but it is very difficult to ignore them. Read more on its remedies.
more infohttp://www.beautyglimpse.com/11-ultimate-home-remedies-for-mouth-and-gum-blisters/

Glossary:Western BlotGlossary:Western Blot

An assay that detects specific proteins within a protein mixture. Samples are subjected to electrophoresis on a slab gel. A replica of the gel is then made on a membrane by electrophoretic transfer. Specific proteins are then detected on the membrane using antibody staining ...
more infohttp://www.informatics.jax.org/glossary/western_blot

Automated Western Blot SystemAutomated Western Blot System

The BlotCycler is capable of running multiple Western blots using two different protocols simultaneously, performing all the ... When blots are removed, the automatic cleaning protocol is employed for thorough cleaning. The system accommodates most ... It is equipped with trays that can be configured to process up to 24 blots with 6 different primary and secondary antibodies ... Users simply add their antibodies and fill the BlotCycler tank with buffer solution, add blot membranes to processing trays, ...
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ECL Western Blotting, need helpECL Western Blotting, need help

... Martin Offterdinger a8803349 at unet.univie.ac.at Thu Jul 31 03:36:41 EST 1997 *Previous ... 2. Stripping of westerns does in my experience only work if the expression of your protein of interest is quite high as you ... it will save you a lot of time to stop using this antibody for Westerns.In my experience it does not make any sense to try ... Previous message: ECL Western Blotting, need help *Next message: ECL Western Blotting, need help ...
more infohttp://www.bio.net/bionet/mm/methods/1997-July/059826.html

Explanation of Western Blot- Brenner - Maryland LymeExplanation of Western Blot- Brenner - Maryland Lyme

Explanation of Western Blot- Brenner Explanation of the Lyme Disease Western Blot. by Carl Brenner. Inquiries about various ... and a Southern blot, which does the same for DNA sequences.. In a Western blot, the testing laboratory looks for antibodies ... A second criticism of the CDC Western blot criteria is that they fail to include the 31 and 34 kDa bands. This does indeed seem ... 2] Dressler F, Whalen JA, Reinhardt BN, Steere AC. Western blotting in the serodiagnosis of Lyme disease. J Infect Dis 1993;167 ...
more infohttps://sites.google.com/site/marylandlyme/lab-tests/explanation-of-western-blot--brenner

Western Blotting Reagents, Membranes, SuppliesWestern Blotting Reagents, Membranes, Supplies

Western Blot Signal Enhancer. Greatly enhance the effectiveness of primary antibodies and intensify the final Western blot ... Thermo Scientific™ Clean-Blot IP Detection Reagents and Kit. Avoid interference from denatured IP antibody fragments in Western ... Thermo Scientific™ Restore™ Western Blot Stripping Buffer Safely and effectively remove primary and secondary antibodies from ... Thermo Scientific™ SuperSignal™ Western Blot Enhancer. Increase both signal intensity and sensitivity 3- to 10-fold with this ...
more infohttps://www.fishersci.com/us/en/products/electrophoresis-westernblottingandelisa/western-blottingreagentsmembranessupplies.html

western blotting - Biochemistrywestern blotting - Biochemistry

western blotting - for longer exposures (Dec/10/2007 ). hallo,. i´m looking for the best chemiluminescence substrate for ... western blotting experiments beyond ECL (longer detection period and stronger signal....).. there are some different on the ...
more infohttp://www.protocol-online.org/biology-forums/posts/32643.html

Western Blot  | Definition | AIDSinfoWestern Blot | Definition | AIDSinfo

Western Blot Western Blot Speaker A laboratory test that detects antibodies for HIV in the blood. The immune system responds to ...
more infohttps://aidsinfo.nih.gov/understanding-hiv-aids/glossary/751/western-blot/

Western Blotting | China-Mainland | Sigma-AldrichWestern Blotting | China-Mainland | Sigma-Aldrich

... using electric current in a process termed as western blotting. Also called as protein blotting or immunoblotting, this ... The various methods of protein detection on western blot are available here.. ... If blocking solution is being prepared, filter the solution to avoid dark grainy background on the blot during development. The ... The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is termed as blotting. ...
more infohttps://www.sigmaaldrich.com/china-mainland/zh/technical-documents/articles/biology/western-blotting.html

Far-western blotting - WikipediaFar-western blotting - Wikipedia

... blotting step. In a western blot, specific proteins are then identified using an antibody probe. Far-western blotting employs ... Far-western blotting is a molecular biological method which is based on the technique of western blotting to detect protein- ... Whereas western blotting uses an antibody to detect a protein of interest, far-western blotting uses a non-antibody protein, ... Thus, whereas western blotting is used for the detection of certain proteins, far-western blotting is rather employed to detect ...
more infohttps://en.wikipedia.org/wiki/Far-western_blotting

ZEA+microarray | Western Blot | ApoptosisZEA+microarray | Western Blot | Apoptosis

The level of P-Erk was examined by Western blot analysis. and the average and S. However.3.2 Ratio (ICI +/-) 1. (B) Inhibition ... p , 0. (C) Quantitative analysis of Western blot data for the inhibition of the phosphorylation of Erk1/2 with ICI 182 780.2383 ... 50 -TTGTGGTTTTCCTGGTGTCA-30 and 50 -CCGAGCTCTGGGACTAATCA-30 . Western blotting The protein was electro-transferred onto ... for 1 h and then visualized with the ECL-plus Western Blotting Detection System (Amersham Pharmacia Biotech. Furthermore.nlm. ...
more infohttps://www.scribd.com/document/249604061/ZEA-microarray

Life Science Technologies: Not Your PIs Western BlotLife Science Technologies: Not Your PI's Western Blot

Reinventing The Western Blot Instead of tweaking the traditional Western blot, ProteinSimples Simple Western has reinvented it ... Not Your PIs Western Blot More than three decades after its invention, the Western blot remains a crucial tool for ... The blotting, antibody incubations, loading, and wash steps account for 80 percent of the time it takes to do a Western blot, ... blotting." Nevertheless, the name stuck, and Western blotting has become one of the most widely used immunochemical techniques ...
more infohttp://www.sciencemag.org/site/products/lst_20130301.xhtml

Western BlotWestern Blot

... fundamentals of western blotting, the reagents youll need, and how BioLegend can help. ... While western blotting may be a time-tested assay, that doesnt mean its simple! Learn more about the ... Western Blotting Introduction. Western blotting refers to a routine technique used to separate and identify proteins from ... For western blotting, sample preparation is a key step in the western blotting process. Proper sample treatment ensures you are ...
more infohttps://www.biolegend.com/en-gb/western-blot

Protocol - Western Blotting ProtocolProtocol - Western Blotting Protocol

Western Blotting Protocol. Reagents. *1X Cell Lysis Buffer: 20 mM Tris-HCl (pH 7.5), 150mM NaCl, 1% NP-40, 2 mM EDTA, 1µg/ml ... Incorrect storage of antibodies or ECL western blotting detection reagents may result in a loss of signal. ... Contamination can be transferred to the blots from electrophoresis and related equipment used in blot preparation. ... Place the wrapped blots, protein side up, in an X-ray film cassette and expose to x-ray film. Exposures can vary from 5 seconds ...
more infohttps://biolegend.com/protocols/western-blotting-protocol/4269/

Western blotting in the serodiagnosis of Lyme disease.  - PubMed - NCBIWestern blotting in the serodiagnosis of Lyme disease. - PubMed - NCBI

Western blotting in the serodiagnosis of Lyme disease.. Dressler F1, Whalen JA, Reinhardt BN, Steere AC. ... There are currently no accepted criteria for positive Western blots in Lyme disease. In a retrospective analysis of 225 case ... Western blotting can be used to increase the specificity of serologic testing in Lyme disease. ... the IgM blot in early disease had a sensitivity of 32% and a specificity of 100%; the IgG blot after the first weeks of ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/8380611

Extracellular Vesicle Isolation and Analysis by Western Blotting | SpringerLinkExtracellular Vesicle Isolation and Analysis by Western Blotting | SpringerLink

Kowal E.J.K., Ter-Ovanesyan D., Regev A., Church G.M. (2017) Extracellular Vesicle Isolation and Analysis by Western Blotting. ... by western blotting.. Key words. Exosomes Extracellular vesicles Exosome isolation Extracellular vesicle isolation ... Ultracentrifugation Immunoblotting Western blot Tetraspanins CD63 CD81 CD9 Emma J.K. Kowal and Dmitry Ter-Ovanesyan contributed ...
more infohttps://link.springer.com/protocol/10.1007%2F978-1-4939-7253-1_12

Western Blotting Reagents | PerkinElmerWestern Blotting Reagents | PerkinElmer

For sensitive and consistent detection of proteins and nucleic acids in blotting applications, count on our wide range of ... Western Blot Chemiluminescence Reagent class="a_dblue"> CDP-Star® Western Blot Chemiluminescence Reagent Star® Western Blot ... Western Lightning ECL Pro NEL120E001EA , NEL122001EA , NEL120001EA , NEL121001EA Western blotting is the most widely used ... Western Lightning™ brand blotting reagents. *Blotting reagents for radiometric, chemiluminescent, chromogenic, and fluorescent ...
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Western blot: Novus BiologicalsWestern blot: Novus Biologicals

Antibody Detection On Western Blot Membranes. Western blotting combines gel electrophoresis with use of a membrane to separate ... Tips on choosing an ideal loading control antibody for Western Blotting. Western blotting is one of the most commonly used ... A New Standard in Antibody Testing - Simple Western Certified Antibodies. The Western blot is one of the most commonly used ... I ran a Western blot with 15ug of a RIPA whole cell lystate from WKPT cells a rat kidney immortalized cell line derived from ...
more infohttps://www.novusbio.com/antibody-news/tag/western-blot

Chemiluminescent Western Blotting | Thermo Fisher Scientific - SAChemiluminescent Western Blotting | Thermo Fisher Scientific - SA

A review of chemiluminescent protein detection for western blotting, including details of the chemiluminescence reaction, ... Figure 6. Use of SuperSignal molecular weight marker for chemiluminescent western blot detection. Western blot analysis of HA- ... Chemiluminescent western blot detection methods The choice of substrate for chemiluminescent western blotting is determined by ... Technical aspects of chemiluminescent western blotting Signal capture. Western blotting is a powerful, routine application; ...
more infohttps://www.thermofisher.com/sa/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/chemiluminescent-western-blotting.html

The Design of a Quantitative Western Blot ExperimentThe Design of a Quantitative Western Blot Experiment

J. E. Gilda and A. V. Gomes, "Stain-free total protein staining is a superior loading control to β-actin for Western blots," ... A. Dittmer and J. Dittmer, "β-Actin is not a reliable loading control in Western blot analysis," Electrophoresis, vol. 27, no. ... K. McDonald, "Increase Western blot throughput with multiplex fluorescent detection: advice and tips on how to quickly generate ... M. Hammond, J. Kohn, K. Oh, P. Piatti, and N. Liu, "A Method for Greater Reliability in Western Blot Loading Controls-Stain- ...
more infohttps://www.hindawi.com/journals/bmri/2014/361590/ref/
  • The following article is offered as a primer in the techniques and interpretation of Western blotting, and should help most patients navigate their way through some of the medical and scientific terminology associated with the assay. (google.com)
  • the Odyssey ® Fc Dual-Mode Imaging System and the C-DiGit ® Blot Scanner. (licor.com)
  • Quantitative fluorescent Westerns (Odyssey Imager) demonstrated that expression of AP-1, which is required for clathrin-coated vesicle formation, is downregulated by hypoxia. (licor.com)
  • The name alludes to Southern blotting (Edwin Southern's 1975 invention of a technique using gel, nitrocellulose, and blotting paper to identify specific DNA sequences in a complex organism), Northern blotting (a similar strategy invented soon afterwards for identifying RNA), and the West Coast location of the Nowinski lab. (sciencemag.org)
  • M. Hammond, J. Kohn, K. Oh, P. Piatti, and N. Liu, "A Method for Greater Reliability in Western Blot Loading Controls-Stain-Free Total Protein Quantitation," Bio-Rad Bulletin: 6360, 2013. (hindawi.com)
  • and a Southern blot, which does the same for DNA sequences. (google.com)
  • Below is an experiment we did to look at the performance differences between imaging the blot correctly (protein side down) and imaging the blot protein side up on the C-DiGit Scanner. (licor.com)
  • The traditional Western blot can be a labor-intensive and time-consuming process, leading many researchers to seek an alternative method that is more efficient, reproducible and quantitative. (novusbio.com)
  • Started dating someone with both HSV1 & 2 and had some oral symptoms, so my doctor ran blood tests again - this time using Western Blot through Quest Labs. (medhelp.org)
  • Try titering it yourself on western 'strips' of the same sample. (bio.net)
  • These properties makes the Western Lightning ECL Pro ideal for use with a wide variety of CCD imagers as well as Kodak BioMax ® Light or X-OMAT Blue film. (perkinelmer.com)