Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Blotting, Southwestern: A method that is used to detect DNA-protein interactions. Proteins are separated by electrophoresis and blotted onto a nitrocellulose membrane similar to Western blotting (BLOTTING, WESTERN) but the proteins are identified when they bind labeled DNA PROBES (as with Southern blotting (BLOTTING, SOUTHERN)) instead of antibodies.Oligonucleotide Probes: Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Collodion: A nitrocellulose solution in ether and alcohol. Collodion has a wide range of uses in industry including applications in the manufacture of photographic film, in fibers, in lacquers, and in engraving and lithography. In medicine it is used as a drug solvent and a wound sealant.DNA Probes: Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Receptors, Cytoplasmic and Nuclear: Intracellular receptors that can be found in the cytoplasm or in the nucleus. They bind to extracellular signaling molecules that migrate through or are transported across the CELL MEMBRANE. Many members of this class of receptors occur in the cytoplasm and are transported to the CELL NUCLEUS upon ligand-binding where they signal via DNA-binding and transcription regulation. Also included in this category are receptors found on INTRACELLULAR MEMBRANES that act via mechanisms similar to CELL SURFACE RECEPTORS.Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.Cyclic AMP: An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.Cyclic AMP Response Element-Binding Protein: A protein that has been shown to function as a calcium-regulated transcription factor as well as a substrate for depolarization-activated CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASES. This protein functions to integrate both calcium and cAMP signals.Cyclic AMP-Dependent Protein Kinases: A group of enzymes that are dependent on CYCLIC AMP and catalyze the phosphorylation of SERINE or THREONINE residues on proteins. Included under this category are two cyclic-AMP-dependent protein kinase subtypes, each of which is defined by its subunit composition.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Teaching Materials: Instructional materials used in teaching.Pharmacology, Clinical: The branch of pharmacology that deals directly with the effectiveness and safety of drugs in humans.Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.Genomics: The systematic study of the complete DNA sequences (GENOME) of organisms.Information Services: Organized services to provide information on any questions an individual might have using databases and other sources. (From Random House Unabridged Dictionary, 2d ed)Biology: One of the BIOLOGICAL SCIENCE DISCIPLINES concerned with the origin, structure, development, growth, function, genetics, and reproduction of animals, plants, and microorganisms.Correspondence as Topic: Communication between persons or between institutions or organizations by an exchange of letters. Its use in indexing and cataloging will generally figure in historical and biographical material.Blotting, Far-Western: A method that is derived from western blotting (BLOTTING, WESTERN) and is used to detect protein-protein interactions. The blotted proteins are probed with a non-antibody protein which can then be tagged with a labeled antibody.Protein Processing, Post-Translational: Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.Glycoconjugates: Carbohydrates covalently linked to a nonsugar moiety (lipids or proteins). The major glycoconjugates are glycoproteins, glycopeptides, peptidoglycans, glycolipids, and lipopolysaccharides. (From Biochemical Nomenclature and Related Documents, 2d ed; From Principles of Biochemistry, 2d ed)Epitopes: Sites on an antigen that interact with specific antibodies.Deltaretrovirus Antigens: Antigens associated with the DELTARETROVIRUS; HTLV-I ANTIGENS and HTLV-II ANTIGENS belong to this group.Deltaretrovirus Antibodies: Antibodies reactive with various types of human T-cell leukemia/lymphoma antigens or bovine leukemia virus antigens.Africa, Central: The geographical area of Africa comprising CAMEROON; CENTRAL AFRICAN REPUBLIC; CHAD; CONGO; EQUATORIAL GUINEA; GABON; and DEMOCRATIC REPUBLIC OF THE CONGO.Human T-lymphotropic virus 1: A strain of PRIMATE T-LYMPHOTROPIC VIRUS 1 isolated from mature T4 cells in patients with T-lymphoproliferation malignancies. It causes adult T-cell leukemia (LEUKEMIA-LYMPHOMA, T-CELL, ACUTE, HTLV-I-ASSOCIATED), T-cell lymphoma (LYMPHOMA, T-CELL), and is involved in mycosis fungoides, SEZARY SYNDROME and tropical spastic paraparesis (PARAPARESIS, TROPICAL SPASTIC).HTLV-I InfectionsBlood DonorsHuman T-lymphotropic virus 2: A strain of PRIMATE T-LYMPHOTROPIC VIRUS 2 that can transform normal T-lymphocytes and can replicate in both T- and B-cell lines. The virus is related to but distinct from HTLV-1.Electrodes: Electric conductors through which electric currents enter or leave a medium, whether it be an electrolytic solution, solid, molten mass, gas, or vacuum.Reagent Kits, Diagnostic: Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.Electrodes, Implanted: Surgically placed electric conductors through which ELECTRIC STIMULATION is delivered to or electrical activity is recorded from a specific point inside the body.Chromogenic Compounds: Colorless, endogenous or exogenous pigment precursors that may be transformed by biological mechanisms into colored compounds; used in biochemical assays and in diagnosis as indicators, especially in the form of enzyme substrates. Synonym: chromogens (not to be confused with pigment-synthesizing bacteria also called chromogens).Treatment Outcome: Evaluation undertaken to assess the results or consequences of management and procedures used in combating disease in order to determine the efficacy, effectiveness, safety, and practicability of these interventions in individual cases or series.Immunoassay: A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.Sensitivity and Specificity: Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)Maf Transcription Factors, Large: A family of high molecular weight Maf transcription factors that contain distinct activation domains.Proto-Oncogene Proteins c-maf: Maf proto-oncogene protein is the major cellular homolog of the V-MAF ONCOGENE PROTEIN. It was the first of the mammalian MAF TRANSCRIPTION FACTORS identified, and it is induced in activated T-LYMPHOCYTES and regulates GENETIC TRANSCRIPTION of INTERLEUKIN-4. c-maf is frequently translocated to an immunoglobulin locus in MULTIPLE MYELOMA.Maf Transcription Factors, Small: A family of Maf Transcription Factors that lack activation domains. Small Maf proteins function as transcriptional repressors or form heterodimeric complexes to serve as transcriptional coactivators. Small Maf proteins include MafF, MafG, and MafK.Diabetes Mellitus, Type 1: A subtype of DIABETES MELLITUS that is characterized by INSULIN deficiency. It is manifested by the sudden onset of severe HYPERGLYCEMIA, rapid progression to DIABETIC KETOACIDOSIS, and DEATH unless treated with insulin. The disease may occur at any age, but is most common in childhood or adolescence.Basic-Leucine Zipper Transcription Factors: A large superfamily of transcription factors that contain a region rich in BASIC AMINO ACID residues followed by a LEUCINE ZIPPER domain.Insulin: A 51-amino acid pancreatic hormone that plays a major role in the regulation of glucose metabolism, directly by suppressing endogenous glucose production (GLYCOGENOLYSIS; GLUCONEOGENESIS) and indirectly by suppressing GLUCAGON secretion and LIPOLYSIS. Native insulin is a globular protein comprised of a zinc-coordinated hexamer. Each insulin monomer containing two chains, A (21 residues) and B (30 residues), linked by two disulfide bonds. Insulin is used as a drug to control insulin-dependent diabetes mellitus (DIABETES MELLITUS, TYPE 1).Histocytochemistry: Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.Endocrinology: A subspecialty of internal medicine concerned with the metabolism, physiology, and disorders of the ENDOCRINE SYSTEM.JapanCell Biology: The study of the structure, behavior, growth, reproduction, and pathology of cells; and the function and chemistry of cellular components.Societies, Scientific: Societies whose membership is limited to scientists.Acetylcholinesterase: An enzyme that catalyzes the hydrolysis of ACETYLCHOLINE to CHOLINE and acetate. In the CNS, this enzyme plays a role in the function of peripheral neuromuscular junctions. EC 3.1.1.7.Staining and Labeling: The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.

An episomally replicating vector binds to the nuclear matrix protein SAF-A in vivo. (1/28)

pEPI-1, a vector in which a chromosomal scaffold/matrix-attached region (S/MAR) is linked to the simian virus 40 origin of replication, is propagated episomally in CHO cells in the absence of the virally encoded large T-antigen and is stably maintained in the absence of selection pressure. It has been suggested that mitotic stability is provided by a specific interaction of this vector with components of the nuclear matrix. We studied the interactions of pEPI-1 by crosslinking with cis-diamminedichloroplatinum II, after which it is found to copurify with the nuclear matrix. In a south-western analysis, the vector shows exclusive binding to hnRNP-U/SAF-A, a multifunctional scaffold/matrix specific factor. Immunoprecipitation of the crosslinked DNA-protein complex demonstrates that pEPI-1 is bound to this protein in vivo. These data provide the first experimental evidence for the binding of an artificial episome to a nuclear matrix protein in vivo and the basis for understanding the mitotic stability of this novel vector class.  (+info)

Identification of different isoforms of eEF1A in the nuclear fraction of human T-lymphoblastic cancer cell line specifically binding to aptameric cytotoxic GT oligomers. (2/28)

GT oligomers, showing a dose-dependent cytotoxic effect on a variety of human cancer cell lines, but not on normal human lymphocytes, recognize and form complexes with nuclear proteins. By working with human T-lymphoblastic CCRF-CEM cells and by using MS and SouthWestern blotting, we identified eukaryotic elongation factor 1 alpha (eEF1A) as the main nuclear protein that specifically recognizes these oligonucleotides. Western blotting and supershift assays confirmed the nature of this protein and its involvement in forming a cytotoxicity-related complex (CRC). On the contrary, normal human lymphocytes did not show nuclear proteins able to produce CRC in a SouthWestern blot. Comparative bidimensional PAGE and Western-blotting analysis for eEF1A revealed the presence of a specific cluster of spots, focusing at more basic pH, in nuclear extracts of cancer cells but absent in those of normal lymphocytes. Moreover, a bidimensional PAGE SouthWestern blot demonstrated that cytotoxic GT oligomers selectively recognized the more basic eEF1A isoform expressed only in cancer cells. These results suggest the involvement of eEF1A, associated with the nuclear-enriched fraction, in the growth and maintenance of tumour cells, possibly modulated by post-translational processing of the polypeptide chain.  (+info)

MFP1 is a thylakoid-associated, nucleoid-binding protein with a coiled-coil structure. (3/28)

Plastid DNA, like bacterial and mitochondrial DNA, is organized into protein-DNA complexes called nucleoids. Plastid nucleoids are believed to be associated with the inner envelope in developing plastids and the thylakoid membranes in mature chloroplasts, but the mechanism for this re-localization is unknown. Here, we present the further characterization of the coiled-coil DNA-binding protein MFP1 as a protein associated with nucleoids and with the thylakoid membranes in mature chloroplasts. MFP1 is located in plastids in both suspension culture cells and leaves and is attached to the thylakoid membranes with its C-terminal DNA-binding domain oriented towards the stroma. It has a major DNA-binding activity in mature Arabidopsis chloroplasts and binds to all tested chloroplast DNA fragments without detectable sequence specificity. Its expression is tightly correlated with the accumulation of thylakoid membranes. Importantly, it is associated in vivo with nucleoids, suggesting a function for MFP1 at the interface between chloroplast nucleoids and the developing thylakoid membrane system.  (+info)

An interaction between a MYC protein and an EREBP protein is involved in transcriptional regulation of the rice Wx gene. (4/28)

We previously demonstrated that a 31-bp nucleotide sequence located upstream of the rice Wx gene played an important role in its expression. We further showed that this cis-acting regulator interacts with nuclear proteins extracted from developing rice endosperm. We used the 31-bp sequence as bait in a yeast one-hybrid system to isolate several cDNA clones from a rice cDNA expression library. One of these cDNAs encodes a MYC protein, designated OsBP-5, which is 335 amino acids long and contains a putative basic helix-loop-helix-ZIP DNA-binding domain. This domain exhibits 50% amino acid sequence identity with the R/B proteins that regulate the expression of genes involved in anthocyanin biosynthesis in plants. The results of electrophoretic mobility shift assays (EMSAs) and Southwestern gel blots indicate that this protein binds specifically to the CAACGTG motif within the 31-bp sequence. However, by itself, the OsBP-5 protein is unable to trans-activate a lacZ reporter gene controlled by the 31-bp sequence when tested in a yeast expression system. Interestingly, OsBP-5 can trans-activate this reporter gene when another protein, OsEBP-89, a member of the EREBP family of transcription factors, is present. Furthermore, in vitro pull-down experiments show that a protein isolated from developing rice endosperm interacts with the OsBP-5 protein, and Western blots confirm that the interacting protein is OsEBP-89. The formation of a supershift band in EMSAs also indicates that two proteins interact with each other. Interference of OsBP-5 gene expression by double-stranded RNA reduces the amylose content in mature seed of transgenic rice plants but has no visible effect on their phenotype. These results suggest that the OsBP-5 and OsEBP-89 proteins act synergistically, perhaps as a heterodimer, to regulate the transcription of the rice Wx gene.  (+info)

Identification of GAPDH as a protein target of the saframycin antiproliferative agents. (5/28)

Saframycin A (SafA) is a member of a class of natural products with potent antiproliferative effects in leukemia- and tumor-derived cells. This activity is frequently conjectured to derive from the ability of saframycins to covalently modify duplex DNA. We used a DNA-linked affinity purification technique to identify GAPDH as a protein target of DNA-small molecule adducts of several members of the saframycin class. Nuclear translocation of GAPDH occurs upon treatment of cancer cells with saframycins, and depletion of cellular GAPDH levels by small interfering RNA transfection confers drug resistance. Roeder and coworkers have recently suggested that GAPDH is a key transcriptional coactivator necessary for entry into S phase. Our data suggest that GAPDH is also capable of forming a ternary complex with saframycin-related compounds and DNA that induces a toxic response in cells. These studies implicate a previously unknown molecular mechanism of antiproliferative activity and, given that one member of the saframycin class has shown efficacy in cancer treatment, suggest that GAPDH may be a potential target for chemotherapeutic intervention.  (+info)

Borrelia turcica sp. nov., isolated from the hard tick Hyalomma aegyptium in Turkey. (6/28)

Previously, a novel, fast-growing spirochaete was isolated from the hard tick Hyalomma aegyptium, which infests tortoises (Testudo graeca), by using Barbour-Stoenner-Kelly (BSK) II medium; the tick samples were taken from the Istanbul area in northwestern Turkey [Guner et al. (2003). Microbiology 149, 2539-2544]. Here is presented a detailed characterization of the spirochaete. Electron microscopy revealed that strain IST7T is morphologically similar to other spirochaetes of the genus Borrelia and possesses 15 to 16 flagellae that emerge from both polar regions. PFGE analysis revealed the genome to comprise a linear chromosome of approximately 1 Mb; two large linear plasmids of approximately 145 and 140 kb, and several small plasmids ranging from 50 to 20 kb in size were also found. The 16S rRNA gene sequence of this Borrelia isolate exhibited 99.4 to 99.8 % identity with other strains isolated from H. aegyptium and less than 99 % similarity with those of other Borrelia species. A phylogenetic tree, generated from 16S rRNA gene sequences, demonstrated that the spirochaete isolates from H. aegyptium clustered together and branched off from both Lyme-disease-related and relapsing-fever-associated Borrelia species. A single copy of the rrs gene was detected in the genome of strain IST7T by Southern hybridization. DNA-DNA hybridization results showed that strain IST7T was distinct from Lyme-disease-related Borrelia, Borrelia burgdorferi and the relapsing-fever-associated species Borrelia hermsii. The G+C content of strain IST7T is 30.0 mol%. From these genetic features, a novel Borrelia species, Borrelia turcica sp. nov., is proposed; the type strain is IST7T (= JCM 11958T = DSM 16138T).  (+info)

Interaction of an approximately 40 kDa protein from regenerating rat liver with the -148 to -124 region of c-jun complexed with RLjunRP coincides with enhanced c-jun expression in proliferating rat liver. (7/28)

The c-jun belongs to the family of proto-oncogenes and encodes for the protein Jun, a component of transcription factor AP-1 involved in regulation of the expression of genes indispensable for cell proliferation and differentiation. While the role of c-jun in the regulation of such genes has been well examined, the regulation of c-jun in proliferating cells is not fully understood. We have earlier reported that the -148 to -124 region of c-jun is involved in the positive regulation of c-jun transcription, and interacts with a positive regulatory factor (rat liver jun regulatory protein; RLjunRP) present in rat liver. In this investigation, we report that this region is differentially recognized in proliferating liver as evidenced by the formation of a complex, different from that observed with normal liver extract. The new complex appears as early as 2 h after partial hepatectomy and its appearance coincides with the rise in c-jun mRNA levels after partial hepatectomy. In regenerating rat liver nuclear extract, an additional protein of approximately 40 kDa (rRLjunRP) interacts with a pre-existing dimer of RLjunRP complexed with the -148 to -124 region of c-jun to form a slow-migrating complex. rRLjunRP appears to pre-exist in the cytosol and translocate to the nucleus as indicated by the continued presence of the retarded complex in nuclear extract prepared from partially hepatectomized rats treated with cycloheximide. UV crosslinking studies, South-Western blot analysis, SDS/PAGE of affinity-purified factor(s), and 2D-PAGE analysis clearly demonstrate that the additional factor induced in response to growth stimulus is an approximately 40 kDa, that binds with the dimer of RLjunRP and enhances the c-jun transcription.  (+info)

A role for UV-light-induced c-Fos: Stimulation of nucleotide excision repair and protection against sustained JNK activation and apoptosis. (8/28)

UV light (UV-C) is a potent inducer of the c-fos gene. Cells lacking c-Fos are hypersensitive to the cytotoxic effect of UV-C indicating a protective role of c-fos induction. Here we show that cells deficient in c-Fos (fos-/-) are unable to remove cyclobutane pyrimidine dimers (CPDs) from DNA and undergo apoptosis at high frequency via the Fas pathway. We also show that in c-Fos-deficient cells the activation of c-Jun N-terminal kinase (JNK) by UV-C differs from the wild-type (wt, fos+/+). In wt cells JNK activation is transient, returning to control level 5-8 h after treatment, whereas in c-Fos-deficient cells JNK activation was long-lasting and did not return to control level. Inhibition of early JNK activation by the JNK inhibitor SP600125 attenuated CPD repair and increased UV-C induced apoptosis whereas inhibition of late JNK activation attenuated the apoptotic response of c-Fos-deficient cells. Late and sustained activation of JNK resulted in upregulation of fas (CD95, apo-1) ligand and induction of caspase 8-dependent apoptosis. The data indicate that the immediate-early induction of the JNK/c-fos pathway stimulates the repair of UV-C induced DNA lesions that protects against apoptosis. If this does not occur, cells do not recover from transcription blockage leading, as shown for c-Fos-deficient cells, to a reduced expression of MKP1, sustained JNK activation and fasL overexpression that finally results in activation of the death receptor pathway. The data support the hypothesis that non-repaired DNA damage is the cause for the late and sustained activation of the MAP kinase pathway in response to genotoxins.  (+info)

Ascoviruses are ds DNA viruses that attack caterpillars and differ from all other viruses by inducing nuclear lysis followed by cleavage of host cells into numerous anucleate vesicles in which virus replication continues as these grow in the blood. Ascoviruses are also unusual in that most encode apoptosis inhibitors and caspase or caspase-like proteins. A robust cell line to study the novel molecular biology of ascovirus replication in vitro is lacking. Therefore, we used strand-specific RNA-Seq to study transcription in vivo in third instars of Spodoptera frugiperda infected with the Spodoptera frugiperda ascovirus, a member of the type species, Spodoptera frugiperda ascovirus (SfAV-1a), sampling transcripts at different time points after infection ...
Southwestern blotting, based along the lines of Southern blotting (which was created by Edwin Southern) and first described by B. Bowen, J. Steinberg and colleagues in 1980, is a lab technique which involves identifying and characterizing DNA-binding proteins (proteins that bind to DNA) by their ability to bind to specific oligonucleotide probes. The proteins are separated by gel electrophoresis and are subsequently transferred to nitrocellulose membranes similar to other types of blotting. The name southwestern blotting is based on the fact that this technique detects DNA-binding proteins, since DNA detection is by Southern blotting and protein detection is by western blotting. However, since the first southwestern blottings, many more have been proposed and discovered. The former protocols were hampered by the need for large amounts of proteins and their susceptibility to degradation while being isolated. "Southwestern blot mapping" is performed for rapid characterization of both DNA-binding ...
Dactyloctenium aegyptium is an annual exotic grass that shows up in disturbed fields. The inflorescence is a panicle with three to six 2-3 cm long spike-like branches all attached at the top of the stem. The spikelets have just a few (usually 2-3) florets. The spikelets are arranged in two rows on the underside of the branches. The second glumes and lemmas may have short awns. The leaf sheath and base of the blade have long hairs ...
We utilized the BAG-one promoter region of an 890-bp DNA as a probe to monitor the HeLa cDNA library, lTripIEx, by Southwestern blot investigation. Following
The protozoan parasite Giardia lamblia is a major cause of waterborne enteric disease worldwide. Lectins are proteins that bind to carbohydrate (sugar) moieties. Potential targets for lectins are found on the surface of most single-celled organisms. Modest concentrations of wheat germ agglutinin (WGA) have been shown to inhibit G. lamblia excystation and trophozoite growth in vitro and can reduce cyst passage in mice infected with the closely related protozoan parasite, G. muris. Commercial preparations of wheat germ (WG) contain 13-53 microg of WGA per gram. We performed a double-masked, placebo-controlled study of dietary supplementation with WG in 63 subjects with giardiasis in Montreal and Lima (25 asymptomatic patients passing cysts; 38 patients with symptoms). Asymptomatic subjects received WG (2 g, 3 times a day) or placebo (cornstarch, 2 g, 3 times a day) for 10 days, followed by metronidazole (250 mg 3 times a day) for 7 days. Symptomatic subjects received metronidazole (250 mg 3 times ...
Bioluminescent-labelling allows sensitive non-invasive sequential imaging of tumor development and early metastasis. Current methods for the genetic modification of cells typically use integrating genotoxic viruses that can potentially disrupt the molecular behavior of cancer cell lines due to their random nature of integration. VAL401 is the reformulation of a clinical drug to enable use in the treatment of cancer. Preclinical data indicate potential use of the reformulated drug in lung cancer, where many subsets of patients have currently a high unmet medical need. We have utilized a non-viral DNA vector that comprises an S/MAR (Scaffold/Matrix Attachment Region) element to stably modify cells to be further used in xenograft studies to allow long term expression without affecting cell behavior or silencing over cell divisions. Human BxPC3 pancreatic cancer cells were stably transfected with a pSMARt-UBC-Luc and cultured for 4 weeks under selection. Colonies that formed after this period were ...
Giardiasis is an intestinal illness caused by infection with the parasite Giardia lamblia, which lives in contaminated water. Although the illness most…
Having giardia, or giardiasis as the condition is medically known, is an unpleasant experience. Even the studys scientific description of it sounds grim: "Giardiasis is an enteric infection with the binucleate trophozite phase of the flagellate protozoan parasite Giardia lamblia." Its acquired when you drink water containing giardia cysts, which then blossom in your gut into the "binucleate trophozite phase" pictured here. This leads to diarrhea, excessive flatulence, foul-smelling excrement, nausea, fatigue, and abdominal cramps. Yuck. (For everything on giardia, heres a comprehensive giardia round-up from Stanford University ...
Substitution of thymine with 5-bromouracil in DNA is known to change interaction between DNA and proteins, thereby inducing various biological phenomena. We hypothesize that A/T-rich scaffold/nuclear matrix attachment region (S/MAR) sequences are involved in the effects of 5-bromodeoxyuridine. We examined an interaction between DNA containing an intronic S/MAR sequence of the immunoglobulin heavy chain gene and nuclear halos prepared from HeLa cells. Upon substitution with 5-bromouracil, the S/MAR DNA bound more tightly to the nuclear halos. The multi-functional nuclear matrix protein YY1 was also found to bind more strongly to 5-bromouracil-substituted DNA containing its recognition motif. These results are consistent with the above hypothesis.. ...
MOUSE, Hassan Ait et al. Evaluation of the in vitro and in vivo anticancer properties of Moroccan propolis extracts. Rev. bras. farmacogn. [online]. 2012, vol.22, n.3, pp.558-567. Epub Mar 08, 2012. ISSN 0102-695X. http://dx.doi.org/10.1590/S0102-695X2012005000030.. This investigation aimed to evaluate the in vitro and in vivo antitumor potential of a Moroccan propolis extracts. For in vitro assays, three mammalian tumor cell lines were used: BSR (hamster renal adenocarcinoma), Hep-2 (human laryngeal carcinoma) and P815 (murin mastocytoma). The propolis ethanolic extract as well as the ethyl acetate extract, exert an in vitro cytotoxic activity in dose-dependent manner. The IC50 values were ranging from 15 µg/mL to 38 µg/mL. This activity depends not only on the extracts chemical composition (analysed by HPLC/ESI-MS), but also on the target tumor cells. Interestingly, the cytotoxic effect of these extracts on the normal human peripheral blood mononuclear cells (PBMC) was weak when compared to ...
E-mail: [email protected], [email protected] Abstract. Salmonella entericais an ultimative human pathogen that causes various health problems. On the other hand, Lactobacillus plantarumhas numerous positive effects on human health. The objective of this study was to investigate the influence of enterobacteriaS. entericaLT21 on human laryngeal carcinoma cell line (HEp2). The ability of the adhesion of S. entericaLT21 as an independent culture and in combination with L. plantarum16.4 to HEp2 cells was tested as well as metabolic activity of S. entericaLT21 after adhesion to HEp2 cells. The protein content in HEp2 cells after the incubation with S. entericaLT21 was investigated and compared with the protein content in the untreated HEp2 cells. Adhesion results show better binding of S. entericaLT21 than L. plantarum16.4 when bacteria are mixed in a 1:1 and 10:1 ratios, respectively. Acetic and lactic acid in the sample of HEp2 cells incubated with S. entericaLT21 were detected, as well as lactic ...
Morrison HG, McArthur AG, Gillin FD, Aley SB, Adam RD, Olsen GJ, Best AA, Cande WZ, Chen F, Cipriano MJ, Davids BJ, Dawson SC, Elmendorf HG, Hehl AB, Holder ME, Huse SM, Kim UU, Lasek-Nesselquist E, Manning G, Nigam A, Nixon JE, Palm D, Passamaneck NE, Prabhu A, Reich CI, Reiner DS, Samuelson J, Svard SG, Sogin ML. (2007) Genomic minimalism in the early diverging intestinal parasite Giardia lamblia. Science. 317:1921-1926 ...
The presence and distribution of the cytoskeletal protein centrin in the lower eukaryote Giardia were determined using a monoclonal antibody to algal centrin. Immunofluorescence shows that centrin is a major component of rodlike structures associated with the flagella and strongly attached to the ve …
Computational modeling is employed to provide a plausible structural explanation for the experimentally-observed differential global genome repair (GGR) propensity of the ALII-N2-dG and ALIIN6- dA DNA adducts of ...
Lexmarks AccuRead Automate app enables a Lexmark smart MFP to capture, automatically classify and route documents, while extracting key information for file naming and indexing.
Giardiasis is an intestinal infection in humans and animals caused by a protozoan parasite Giardia intestinalis . Giardia is a simple one-celled parasitic species; it is not a
Diarrheal disease presents a debilitating burden on many people and economies around the world. Many of these cases are caused by the protozoan parasite Giardia sp., which colonizes the small intestine of a wide range of hosts and disrupts normal digestive and absorptive processes within the gut. Malnutrition caused by giardiasis is largely immune-dependent; the pathology commonly observed within the infected small intestine is likely triggered by activated CD8+ T cells. Understanding the dynamic immune response to Giardia infection is paramount for eventually identifying therapeutic targets and improving preventative measures. We report an influx of FasL+ cells into the duodenum following infection in C57BL/6 mice. Ex vivo restimulation of splenic and mesenteric lymph node (MLN) lymphocytes revealed an IFN- dominant cytokine response, which is conducive for the expansion of activated, FasL expressing CD8+ T cell. This observation prompted us to explore the role of Fas as a potential mediator ...
Quantification of the interaction of Tc with dissolved boom clay humic substances. The effect of chromosome 7B on the level of amylose suggested the presence of a regulator gene which suppresses the activities of the Wx genes. Controlled studies are necessary to evaluate and to compare the efficacy of tadalafil price each laser, in order to determine their optimal indications and optimal parameters for each machine. Vesicourethral function in mice with genetic viagra or cialis disruption of neuronal nitric oxide synthase. For a mixture of historical and practical reasons, much of the bioinformatics software discussed in this series runs on Linux, Mac OSX, Solaris, or one of the many other Unix variants.. Issues in co-morbid viagra vs cialis comparison chart severe mental illnesses in HIV infected individuals. Clinical value of p16/Ki-67 immunocytochemical dual staining in cervical cancer screening Intracellular tetanic calcium signals are reduced in fatigue of whole skeletal muscle.. In most ...
Inniu (15 Feabhra 2008) dainmnigh Údarás na hOllscoile Ollscoil na hÉireann, Gaillimh an tOllamh Jim Browne (55) mar Uachtarán nua na hOllscoile. I láthair na huaire tá an tOllamh Browne ag feidhmiú mar Mheabhránaí agus Uachtarán Ionaid in OÉ Gaillimh agus cuirfidh sé tús lena phost nua mar Uachtarán an 6 Márta 2008. Is é an tOllamh Browne an dara hUachtarán déag atá ceaptha san Ollscoil agus mairfidh a théarma oifige deich mbliana. Dúirt an Dr Noel Dorr, Cathaoirleach an Bhoird Roghnúcháin a cheap Údarás na hOllscoile go bhfuil "cáil bainte amach ag an Ollamh Browne mar scoláire mór le rá, tá teagmhálacha aige ar fud na cruinne agus tá taithí aige ar Ollscoileanna eile san Eoraip, sna Stáit Aontaithe, san Áise agus Meiriceá Laidineach. Tá go leor taighde déanta aige féin agus tuigeann sé an tábhacht a bhaineann le tacú le taighde san Ollscoil. Lena chois seo, tá sé mar aidhm aige luachanna traidisiúnta na hOllscoile a chaomhnú i réimse na ...
The human body is a mystery. As much and as fast as were learning, we still know very little. This post is about a recent discovery. I call it a "discovery," because Ive lived out what this theory holds to be true, as have a number of patients I work with, and perhaps some of you reading this. RCCX theory is quite complex. The best I can do is share my personal experience with the hope that doing so will help make this information more accessible. ...
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Vibrio cholera, causing acute watery diarrhea known as cholera disease, affects all ages and both genders. Cholera infection outbreaks in Iraq have been reported for several years. The recent cholera outbreak, emerged throughout 2015, was investigated using bacteriological laboratory tests, singleplex and multiplex PCR technique for the detection of V. cholera from stool samples. Furthermore the toxigenic potential coupled with the antibiotic susceptibility test for cholera and other bacteria were also investigated. The stool samples were collected from 5698 patients admitted to Al-Yarmouk Teaching hospital and health care centers in Baghdad/Al-Karkh, Iraq, from the 1st of August to the 30th of December 2015. The V. cholera was isolated from 194 cases (3.4% of the cases age between 21 - 50 years). In addition, other enteric infections: Salmonellosis and Shigellosis 7 and 21 respectively, protozoan parasite Giardia lamblia and Entamoeba histolytica 2 and 43 cases respectively were also reported. High
Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a transcriptosomal complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing. When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter (By similarity).
The protozoan parasite Giardia intestinalis (syn. G. lamblia, G. duodenalis) is one of the most common causes of diarrheal disease throughout the world, where an estimated 500 million people are infected annually. Despite efforts in trying to elucidate factors associated with virulence in G. intestinalis little is currently known. The disease outcome is highly variable in Giardia infected individuals, ranging from asymptomatic carriers to severe disease. The reasons behind the differences in disease outcome are vaguely understood and studies trying to link infectivity to different Giardia assemblages or sub-assemblages have rendered conflicting results. Prior to this study, little was known about the prevalence and genetic diversity of different G. intestinalis assemblages across the world.. In this thesis, molecular characterization of clinical G. intestinalis samples from Eastern Africa and Central America, has been performed, enabling a better understanding of the prevalence of different ...
Fernández Alarcón, Vicenç; Simó Guzmán, Pep; Salán Ballesteros, Maria Núria; Algaba Joaquín, Inés María; Enache, Cristina Mihaela; García-Almiñana, Daniel; Albareda Sambola, Maria; Suñé Torrents, Albert; Bravo Ibarra, Edna Rocío; Amante García, Beatriz; Rajadell Carreras, Manuel; Garriga Garzón, Federico; Serrano, Pilar; Marcé Nogué, Jordi; Pepió Viñals, Montserrat; Sallán Leyes, José María (Universitat Politècnica de Catalunya. Institut de Ciències de lEducació, 2010-02-11) ...
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In molecular biology, the BEN domain is a protein domain which is found in diverse proteins including: SMAR1 (Scaffold/Matrix attachment region-binding protein 1; also known as BANP), a tumour-suppressor MAR-binding protein that down-regulates Cyclin D1 expression by recruiting HDAC1-mSin3A co-repressor complex at Cyclin D1 promoter locus; SMAR1 is the target of prostaglandin A2 (PGA2) induced growth arrest. NACC1, a novel member of the POZ/BTB (Pox virus and Zinc finger/Bric-a-bracTramtrack Broad complex), but which varies from other proteins of this class in that it lacks the characteristic DNA-binding motif. Mod(mdg4) isoform C, the modifier of the mdg4 locus in Drosophila melanogaster (Fruit fly), where mdg4 encodes chromatin proteins which are involved in position effect variegation, establishment of chromatin boundaries, nerve path finding, meiotic chromosome pairing and apoptosis. Trans-splicing of Mod(mdg4) produces at least 26 transcripts. BEND2, a protein of unknown function, that is ...
Health, This press release is available in A HREF http://www.eurek...The intestinal parasite Giardia lamblia changes outfits nearly as ofte...Now Hugo Lujn a Howard Hughes Medical Institute international researc...Lujn believes Giardias hardy surface proteinswhich help the parasite ...A professor at the Catholic University of Crdoba in Argentina Lujns...,New,vaccines,may,come,from,forcing,giardia,parasite,to,display,its,many,disguises,medicine,medical news today,latest medical news,medical newsletters,current medical news,latest medicine news
Dr. Andrea Rópolo received his Ph.D. from the National University of Córdoba, Argentina in 2001 and then subsequently performed her post-doctoral training studying different aspect of the antigenic variation process in Giardia, a parasitic protozoan of humans and other vertebrates and a major source of waterborne disease worldwide. Dr. Rópolo currently serves as an Investigator in the Laboratory of Microbiology and Immunology in the Ferreyra Research Institute of Córdoba, Argentina. She is also Assistant Professor in the National University of Córdoba. His current researches focus on epigenetic mechanisms involved in the biology of the parasite Giardia lamblia. Particularly, her team wants to understand how the SUMOylation of proteins and the deimination (citrullination) of arginines regulate growth and cyst differentiation in Giardia. She also leads the production of monoclonal antibodies against recombinant proteins, peptides and native proteins in the Ferreyra Research Institute for ...
matrin 3 an acidic protein that is a component of the nuclear matrix. May play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. Note: This description may include information from UniProtKB ...
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ABSTRACT. Malignant theileriosis of sheep is a highly fatal, acute or subacute disease is caused by the tick-borne protozoan parasite, Theileria hirci. In this investigation ten healthy male lambs aged 5-6 months were randomly divided into two groups, A and B and were kept in isolated tick-proof pens. They were treated for internal and external parasite before commencement of the experiment. The lambs were experimentally infected with T. hirci by placing ticks Hyalomma anatolicum anatolicum infected with T. hirci on them. The ticks used in this survey had originally been isolated from sheep and colonies of them were established in an insectarium. Before and after infection rectal temperatures and clinical signs of the lambs were recorded, blood and prescapular lymph node smears were prepared and examined to determine the extent of the parasitaemia, and blood samples were analyzed to evaluate their haemoglobin (Hb) and packed cell volume (PCV) rates. Three days after the commencement of a febrile ...
Alpha-giardins constitute the annexin proteome (group E annexins) in the intestinal protozoan parasite Giardia and, as such, represent the evolutionary oldest eukaryotic annexins. The dominance of alpha-giardins in the cytoskeleton of Giardiawith its greatly reduced actin content emphasises the importance of the alpha-giardins for the structural integrity of the parasite, which is particularly critical in the transformation stage between cyst and trophozoite. In this study, we report the crystal structures of the apo- and calcium-bound forms of a1-giardin, a protein localised to the plasma membrane of Giardia trophozoites that has recently been identified as a vaccine target. The calcium-bound crystal structure of a1-giardin revealed the presence of a type III site in the first repeat as known from other annexin structures, as well as a novel calcium binding site situated between repeats I and IV. By means of comparison, the crystal structures of three different alpha-giardins known to date ...
PHELAN, THOMAS JOSEPH, Genetic and Molecular Analysis of Plant Nuclear Matrix Proteins. (Under the direction of Steven L. Spiker.)The eukaryotic nucleus is composed of DNA, RNA and protein, encapsulated by a nuclear envelope. DNA is compacted up to ten thousand times in order to be packaged into the nucleus. The nucleus must maintain order in the presence of a very high density and variety of protein and RNA. The nuclear matrix is a proteinaceous network thought to provide structure and organization to the nucleus. We believe that relatively stable interactions of nuclear molecules with the nuclear matrix are key to organization of the nucleus. Numerous "Matrix Attachment Region" DNA elements (MARs), have been isolated from plants, animals, and fungi. Evidence suggests that these MARs attach to the nuclear matrix, delimiting loops of chromosomal DNA. In studies of transgenic plants and animals, MARs have been shown to give important advantages to organisms transformed with genes flanked by these ...
Aims/hypothesis High birthweight and increased childhood growth are risk factors for type 1 diabetes. Relative birthweight is associated with HLA genotypes that confer a high risk of diabetes. Our...
The key proposals were: Regarding new technologies, option 3 best supports the effectiveness of the legislative framework at this time. Under option 3 organisms modified using site-directed nucleases without templates to guide genome repair (i.e. SDN-1) would not be regulated as GMOs. Currently, if a template is used to guide genome repair (i.e. SDN-2 and SDN-3), the resulting organisms are GMOs, as are organisms modified using oligonucleotide-directed mutagenesis. These would continue to be regulated under this option ...
(Medical Xpress)-A protein called Myc, commonly found at high levels inside cancer cells, fuels the disease by allowing cells to override their in-built self-destruct mechanisms, according to two new studies by US scientists.
... dynamics Mass spectrometry Isotopic labeling Coimmunoprecipitation Electrophoretic mobility shift assay Southwestern blotting ... reaction Northern blotting Southern blotting Fusion proteins DNA microarray Bioinformatics flow cytometry Western blotting ... Southern blot -- fractionation -- Gram stain -- Surface Plasmon Resonance -- Microscale Thermophoresis DNA sequencing ...
"Southwestern Air Express to Be Started Next Week" (Google news archive). St. Joseph Gazette. March 30, 1929. Retrieved 2013-07- ... https://books.google.com/books?id=Q7YIp8f69rMC&pg=PA223&lpg=PA223&dq=fairfax+%22air+depot%22+kansas&source=bl&ots=VH4dKVkUzN& ...
... bl&ots=DNdhTtgINZ&sig=fXrDak1Ub4CBlwgEV1aAk7BRC3A&hl=en&sa=X&ved=0ahUKEwirn9D4vM3KAhWPsh4KHd6OA5YQ6AEIKDAC#v=onepage&q= ... Treated effluent from the Rabbit Lake uranium mine is released into Hidden Bay on the southwestern side of the lake. https:// ... books.google.com/books?id=uPgDwqflBNsC&pg=PA66&lpg=PA66&dq=Wollaston+Lake+Cochrane+River&source=bl&ots=DNdhTtgINZ&sig= ...
... slot blot - SNP - snRNA - snRNP - solution hybridization - somatic cells - Southern blot - southwestern blot - SP6 RNA ... blotting - blunt end - bone marrow transplantation - box - BP - BRCA1 - BRCA2 - C terminus - cancer - candidate gene - ... Western blot - wildtype - wobble position - Wolfram syndrome - YAC (yeast artificial chromosome) zinc finger - Index of ... genomic blot - genomic clone - genomic library - genotype - germ line - glycoprotein - glycosylation - Golgi apparatus - GRE - ...
Southern blot Western blot Northern blot Southwestern blot Eastern blot Gel electrophoresis SDS-PAGE Chromatography ... These techniques include the Western blot (protein detection), the Northern blot (RNA detection), the Southwestern blot (DNA- ... C, Franke; Grafe D; Bartsch H; Bachmann M (2009). "Use of non-radioactive detection method for north- and southwestern blot". ... The Northwestern blot allows for detection of identified proteins in a way that is inexpensive. The blot is typically a first ...
Southwestern blotting, based along the lines of Southern blotting (which was created by Edwin Southern) and first described by ... since DNA detection is by Southern blotting and protein detection is by western blotting. However, since the first southwestern ... "Southwestern blot mapping" is performed for rapid characterization of both DNA-binding proteins and their specific sites on ... The name southwestern blotting is based on the fact that this technique detects DNA-binding proteins, ...
The method is similar to South-western blotting. (2006) Eastern blotting has been used to refer to the detection of fusion ... Far-western blotting Far-eastern blotting Glycosylation Phosphorylation Northwestern blot Western blot Northern blot Southern ... The method is similar to South-western blotting. (2011) A recent study used the term eastern blotting to describe detection of ... Since this is essentially a western blot, the charge reversal was used to dub this method an eastern blot. (2005) Eastern blot ...
J. C. Peters, M.D., "The South Western Cholera: 1873, The Sanitarian", Sept. 1873, National Institutes of Health exhibit, ... https://books.google.com/books?id=tphHxMxRJmMC&pg=PA704&lpg=PA704&dq=Cholera+deaths+in+1873+Sumner+Co,+TN&source=bl&ots= ...
... bl&ots=SmoSG6KwPA&sig=KLzOZ8nnl10Gj1KnjATzD_mFLI0&hl=en&sa=X&ved=0ahUKEwjElOPSgdfKAhVlLqYKHWdpApoQ6AEIODAF#v=onepage&q&f=false ... Minister for Public Transport 12 September 2007 V/Line central region weekday network service plan August 2017 V/Line south western ...
At the time, as red shirt freshman, Turner led the Southwestern Athletic Conference with nine interceptions. He set a national ... bl&ots=sFyoQAfoOB&sig=8-u84D01RBF7b5WLfDn9uz7VeNE&hl=en&sa=X&ved=0ahUKEwjJqZmw0NDUAhWFbT4KHd7wC_AQ6AEIRTAI#v=onepage&q=Tyophi% ... where he earned Division I-AA All America and All Southwestern Athletic Conference Honors in 1990. After his breakout season, ...
Southwestern Baptist Theological Seminary, M.A. - Dallas Baptist University In 2010 Wilder in partnership with BorderStone ... https://books.google.com/books?id=p3dT6ZPr_mkC&pg=PA8-IA4&lpg=PA8-IA4&dq=Terry+L.+Wilder,+apologetics+study+bible&source=bl&ots ... Terry L. Wilder is professor of New Testament at Southwestern Baptist Theological Seminary in Fort Worth, Texas. He previously ... http://www.swbts.edu/index.cfm?pageid=800&enc=4D5E4B4A5433392C234425504151415C3A283A215463 Southwestern Baptist Theological ...
Fields, Dorothy Louis (October 1945). "David Gouverneur Burnet". Southwestern Historical Quarterly. Texas State Historical ... https://books.google.com/books?id=B1wUAAAAYAAJ&pg=PA620&lpg=PA620&dq=1841+%2B+Texas+%2B+7,915+%2B+president&source=bl&ots= ... The Southwestern Historical Quarterly. Texas State Historical Association. 23 (3): 168-170. JSTOR 27794561. Southwick, Leslie ( ...
... is located just off the southwestern coast of the main island of Fiji known as Viti Levu. Namotu Island Resort, a haven ... bl&ots=NVHrNcKrlZ&sig=RHIkaEwFernSTWLR0kenoObKg1s&hl=en&sa=X&ei=Tak3U5_tJunuyQHGj4DIDQ&ved=0CDoQ6AEwCA#v=onepage&q=malolo% ...
Other locations named Cape Smyth are: (1) the south-western point of Melville Island, between Warrington Bay and Hardy Bay, in ... bl&ots=AUymog872d&sig=O4Lw1JkRG8CzxC_ZVUh0Vxs5rxw&hl=en&sa=X&ved=0ahUKEwiTt5bPtc_TAhVnKMAKHbCCCg4Q6AEIMDAD#v=onepage&q=cape% ...
Georgia Southwestern State University Archived 2010-07-06 at the Wayback Machine., Retrieved June 26, 2010. South Georgia ... 73-74https://books.google.com/books?id=svf_7DV9i6UC&pg=PA129&lpg=PA129&dq=remembering+americus+georgia+essays&source=bl&ots= ... It is a part of the Georgia Independent Schools Association (GISA). Georgia Southwestern State University- Main Campus South ... now Georgia Southwestern State University), and the South Georgia Trade and Vocational School in 1948 (now South Georgia ...
Outside Africa where cleavers were most abundant, cleavers have appeared in Southwestern Europe. In these regions, they are ... ISBN 0-19-280005-1. https://books.google.com/books?id=lRDYAW4wXOYC&pg=PA54&lpg=PA54&dq=archaeology+cleaver&source=bl&ots= ...
Joe's Southwestern influence is said by some to explain the spread's spicy nature. On February 21, 2013, the Kentucky ... bl&ots=-QlvRw8bq_&sig=vv_f5NPNFQKxSJs4v-SgMUUkPGo&hl=en&sa=X&ved=0ahUKEwizwPa1uaTRAhVKzoMKHZNnA-UQ6AEIVDAN#v=onepage&q=queen% ...
It was indicated through Western blot that cyclin D1 was upregulated from day 1 to day 60 post-infection. It was also indicated ... T. cruzi transmission has been documented in the Southwestern U.S., and warming trends may allow vector species to move north. ...
On the southwestern Coast of South India, a new local economical and political power arose into the vacuum created by the ... 49-51 https://books.google.com/books?id=yK8D8c5-HXAC&pg=PA126&lpg=PA126&dq=Garikapadu+stupa&source=bl&ots=RSa1g5lrqQ&sig= ... Mysore State was enlarged by the addition of Coorg and the Kannada-speaking districts of southwestern Hyderabad State and ...
The Buocher Höhe is usually seen as the area on the southwestern edge of the forested upland of Berglen, which is sometimes ... Below this thin layer lies Rhätsandstein, which was formerly milled into blotting sand for the drying of ink. The old quarry ...
This name has been used at race tracks in the Western PA/WV/Southwestern NY area and the GA/AL/SC area. In Australia, the term ... https://books.google.ca/books?id=eR019JqPbBkC&pg=PA89&lpg=PA89&dq=South+Africa+cadets+apartheid&source=bl&ots=yzsj-l-OEW&sig= ...
... bl&ots=dwlJXEEO_B&sig=Ezg_EQ380mVcUTN8XY15Yg3IOgQ&hl=en&sa=X&ved=0ahUKEwidqrCZidXQAhVi2IMKHXziAAAQ6AEIKDAC#v=onepage&q=sam% ... A collection of Gwynn's papers is housed at Texas State University at San Marcos in the Southwestern Writers Collection. A link ...
Farah was born in 1926 in the city of Bardera in Gedoregion in south western Somalia. The poet studied poetry from other famous ... bl&ots=R3oVuMa07c&sig=zK55RhDiHiyJEOUQzxQQNX_VHn0&hl=en&sa=X&ei=_qmZVJ6jH4KvoQSgkoHABQ&ved=0CDMQ6AEwBA#v=onepage&q=farah% ...
... located in the Tarfaya Province of the Laâyoune-Sakia El Hamra region of southwestern Morocco. It is a port town on the ... bl&ots=1xP8M2TSGx&sig=uy3IBoLfutTu8JIWBn09id-wiG0&hl=en&sa=X&ved=0ahUKEwi2zP-3yOfYAhUW6mMKHVaYBN4Q6AEIQzAJ#v=onepage&q=Treaty% ...
The PCR and dot blot hybridization (DBH) test was used in 2006-2007. 742 ducks from 70 duck farms were tested. The overall ... "Complete Genome Sequence of the Novel Duck Circovirus Strain GH01 from Southwestern China". Genome Announcements. 1 (1): e00166 ... Scientists have studied the Duck circovirus by using polymerase chain reaction (PCR)-based methods and dot blot hybridisation ( ...
Nowadays khat consumption is limited to East Africa and South Western Arabia. These countries include Djibouti, Ethiopia, Kenya ... "Drugs and dance as Israelis blot out intifada". The Guardian.. *^ Bentur, Y.; Bloom-Krasik, A.; Raikhlin-Eisenkraft, B. (2008 ...
Southwestern blotting, based along the lines of Southern blotting (which was created by Edwin Southern) and first described by ... since DNA detection is by Southern blotting and protein detection is by western blotting. However, since the first southwestern ... "Southwestern blot mapping" is performed for rapid characterization of both DNA-binding proteins and their specific sites on ... The name southwestern blotting is based on the fact that this technique detects DNA-binding proteins, ...
Proteins are separated by electrophoresis and blotted onto a nitrocellulose membrane similar to Western blotting (BLOTTING, ... Southwestern" by people in this website by year, and whether "Blotting, Southwestern" was a major or minor topic of these ... "Blotting, Southwestern" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical ... Below are the most recent publications written about "Blotting, Southwestern" by people in Profiles. ...
Southwestern Blot and UV Cross-Linking Analysis of CRE-RIIβ Binding.. Southwestern blot analyses (16) and UV cross-linking (17 ... Southwestern Blot Analysis.. Southwestern blot analysis was performed as described by Silva et al. (16) with minor ... Southwestern blot analysis and UV cross-linking assay demonstrate RIIβ binding to CRE oligonucleotide. (A) Southwestern blot ... 2 B, lanes 3 and 5, and C, lane 4). Both Southwestern blot analysis and UV cross-linking demonstrated that, apart from RIIβ and ...
There is Northern, Southern, Western Southwestern etc. http://en.wikipedia.org/wiki/Northern_blot ... Northern blotting takes its name from its similarity to the first blotting technique, the Southern blot, named for biologist ... However, the entire process is commonly referred to as northern blotting. The northern blot technique was developed in 1977 by ... The term northern blot actually refers specifically to the capillary transfer of RNA from the electrophoresis gel to the ...
The method is similar to South-western blotting. (2006) Eastern blotting has been used to refer to the detection of fusion ... Far-western blotting Far-eastern blotting Glycosylation Phosphorylation Northwestern blot Western blot Northern blot Southern ... The method is similar to South-western blotting. (2011) A recent study used the term eastern blotting to describe detection of ... Since this is essentially a western blot, the charge reversal was used to dub this method an eastern blot. (2005) Eastern blot ...
Western and Southwestern Blot Analyses. Proteins were separated by SDS/PAGE in 4-15% gels (Bio-Rad) and transferred onto the ... SW, Southwestern blot analysis of the fraction eluted with 300 mM KCl, using the 32P-labeled TSE probe. (D) Immuno-EMSA of the ... For the Southwestern blot analysis, membranes were soaked for 1 h at room temperature in buffer D containing 6 M guanidinium ... analyzed by Western blotting. The antibodies used for immunoprecipitation (IP) and Western blot analysis (WB) are indicated at ...
Blotting, Southwestern. A method that is used to detect DNA-protein interactions. Proteins are separated by electrophoresis and ... BLOTTING, WESTERN) but the proteins are identified when they bind labeled DNA PROBES (as with Southern blotting (BLOTTING, ... DNA analysis, Southern blotting, cytogenetic analysis, polymerase chain reaction. Location. Naval Medical Center - San Diego. ... Compare quantitative RT-PCR and quantitative Southern blot results with marrow cytogenetics at the time of complete molecular ...
Blotting, Southwestern. A method that is used to detect DNA-protein interactions. Proteins are separated by electrophoresis and ... Blotting, Far-western. A method that is derived from western blotting (BLOTTING, WESTERN) and is used to detect protein-protein ... BLOTTING, WESTERN) but the proteins are identified when they bind labeled DNA PROBES (as with Southern blotting (BLOTTING, ... The blotted proteins are probed with a non-antibody protein which can then be tagged with a labeled antibody. ...
Western blotting. Twenty-five micrograms of protein lysate per lane were used for SDS-PAGE gels. Western blots were transferred ... The animal study was approved by the Institutional Animal Care and Use Committee (IACUC) at UT Southwestern Medical Center. All ... Western blots, specific activity, and histology. (A) Western blots showing phosphorylated PDH E1α subunit (pE1α), total ... Western blot analyses of tissue samples (Fig. 6A) indeed showed that phosphorylation of the E1α subunit was very low in DIO DKO ...
Southern blot Western blot Northern blot Southwestern blot Eastern blot Gel electrophoresis SDS-PAGE Chromatography ... These techniques include the Western blot (protein detection), the Northern blot (RNA detection), the Southwestern blot (DNA- ... C, Franke; Grafe D; Bartsch H; Bachmann M (2009). "Use of non-radioactive detection method for north- and southwestern blot". ... The Northwestern blot allows for detection of identified proteins in a way that is inexpensive. The blot is typically a first ...
I think Ausubel says so) - Northwestern or Southwestern also exists iirc. But Eastern - youd have to come up with a fourth ... obvious Southern blot), and yes its for a lecture and there will be , students asking it... so if you know of even more blot ... Bruno Cenni wrote: , Hi all, , , I was wondering where the different blot names come from (apart from the , ... Western & Eastern & Far Western & other blots. Susanne Rohrer srohrer(removethis) at immv.unizh.ch Fri Oct 13 05:30:41 EST ...
1988) Antibodies to HTLV-I in populations of the southwestern Pacific. J. Med. Virol. 26:339-351. ... 1990) Low incidence of HTLV infections in random blood donors with indeterminate western blot patterns. Transfusion 30:491-494. ... 1999) HTLV-I/II seroindeterminate Western blot reactivity in a cohort of patients with neurological disease. J. Infect. Dis. ... These data suggest that in Central Africa, this frequent and specific Western blot is not caused by HTLV-1 infection but could ...
Read independent reviews on Trans-Blot® Cell With Plate Electrodes and Super Cooling Coil (170-3939) from Bio-Rad on ... Organization: UT SOUTHWESTERN MEDICAL CTR.. * Ease of use. 5 out of 5 After sales service. 5 out of 5 Value for money. 5 out of ... The Trans-Blot® Transfer Cell is a versatile apparatus ideal for a wide variety of blotting applications. Features and Benefits ... Electrodes can be positioned 8 cm apart for standard blots or 4 cm apart for high-intensity blots ...
Blotting procedure for HIV confirmation. A. Southern Blot. B. Northern Blot. C. Western Blot. D. Southwestern Blot. ... Southern Blot is for = Northern Blot is for = Western Blot is for = ... C. Western Blot. ***Specific and confirms HIV. ***1st: ELIZA is sensitive but not specific ...
Southwestern blot identifies DNA binding proteins. --with labeled oligonucleotides. --Example: Leucine zipper. ...
B) Southwestern blotting using the IM probe. (C) Southwestern blotting using the HM* probe, which contains multiple mutations ... A to C) (Left panels) Southwestern blotting experiment performed with His-tagged ZI and ZA proteins from total E. coli extracts ... By southwestern analysis, PRDM9A protein (labeled ZA) was shown to have high affinity to a DNA fragment including the 13-mer ... Right panels) Mirror-image blots obtained after diffusion transfer to a membrane placed on the other side of the same protein ...
Southwestern dot blot analysis of CPDs. The gDNA was isolated from the different treatment groups of BM-DCs following standard ... This was verified using dot blot analysis and that this form of DNA damage was not apparent in the DCs that were not UVB- ... C, analysis of CPDs by dot blot assay. Results are shown from a single experiment that is a representative of 2 separate ... was transferred to a positively charged nitrocellulose membrane by vacuum dot-blotting (Bio-Dot Apparatus, Bio-Rad). The ...
... burgdorferi containing bbe02 or bbq67 when used in a Southwestern blot format (Fig. 5). Dot blot assays with the same antiserum ... B) Southwestern blot analysis of plasmid DNA from B. burgdorferi and E. coli. The gel shown in panel A was transferred to a ... B) Southern blot analysis of RsaI-digested plasmid DNA from B. burgdorferi and E. coli. The gel shown in panel A was ... DNA from all strains in a dot blot assay but had a high level of nonspecific background when used in the Southwestern format ( ...
RIPE3b1 binding activity is detected at pH 4.5 and pH 7.A Southwestern blotting strategy was developed to determine the ... Two-dimensional (2-D) gel electrophoresis and Southwestern blotting.The DNA affinity-purified RIPE3b1 binding fractions and βTC ... A) Southwestern analysis was performed by using 2-D electrophoresis resolved βTC-3 nuclear extract (NE). InsC1 binding of a 46- ... Immunoblot analysis.Large Maf expression was analyzed by Western blotting with antisera specific to MafA (1:10,000 dilution), c ...
Recent Advances in Molecular Histochemical Techniques: In Situ Hybridization and Southwestern Histochemistry * * Koji Takehiko ... Use of a protein-blotting procedure and a specific DNA probe to identify nuclear proteins that recognize the promoter region of ... Localization of cyclic adenosine 3,5-monophosphate-responsive element (CRE)-binding proteins by southwestern histochemistry ... A reliable method for northern blot analysis using synthetic oligonucleotide probes. HENDERSON GS. ...
Examples of techniques normally performed on specific surfaces include western blotting, far-western blotting, southwestern ... Southwestern blotting is used to detect the interaction of a nucleic acid (such as DNA or RNA) with a protein. After transfer ... After the rinse, excess PBS is removed from the slide by briefly blotting its edge with an absorbent paper towel. The slide is ... The membrane is then processed with primary and secondary antibodies as in any standard western blotting protocol. ...
The same blots were probed with STAT3-specific Abs to compare the total levels of STAT3 in wild-type and IRAK-1-deficient T ... James Thomas (University of Texas Southwestern Medical School, Dallas, TX). ApoE−/−/IRAK-1−/− mice were obtained by breeding ... The same blots were probed with LaminB-specific Abs as a marker for loading control. B, The expression of NFATc2 was analyzed ... Western blotting analysis of the protein samples were performed as described previously (21). Immunoblots were developed by ...
Use of Nonradioactive Detection Method for North- and South-Western Blot.. Franke C, Gräfe D, Bartsch H, Bachmann MP. ...
E) IP of the indicated CHD2-GFP fragments from HEK293 cells followed by southwestern blotting to monitor association with ... H) Western blot on U2OS CHD2-GFP cells.. (I) SILAC of HEK293 cells expressing GFP (L) or CHD2-GFP (H).. (J) CoIP of CHD2-GFP ... B) Western blot showing expression of the mutants from A in U2OS cells.. (C) Recruitment of CHD2-GFP deletion mutants in cells ... E) Western blot showing PARP1/2 knockdown efficiency in cells from (D).. (F) Quantification of (D). 30-170 cells were analyzed ...
Other similar techniques such as far-western blots and south-western blots allow scientists to examine protein-protein ... The Northern blot, also known as the RNA blot, is one of the blotting techniques used to transfer DNA and RNA onto a carrier ... Northern Blot is a derivative of the Southern blot, which, like the Southern Blot, utilizes electrophoresis separation analysis ... Western Blot and Others[edit]. Western blots allow investigators to determine the molecular weight of a protein and to measure ...
  • For example, many scientists researching Huntington disease or breast cancer are able to determine the expression patterns of the genes responsible for these diseases using blotting techniques. (wikibooks.org)
  • Monitor molecular response rates of patients receiving treatment for chronic myelogenous leukemia by quantitative reverse transcription polymerase chain reaction (RT-PCR) and quantitative Southern blot monitoring of blood samples. (bioportfolio.com)
  • It is mRNA which is isolated and hybridized in northern blots.The formaldehyde was use in electrophoresis gel as a denaturant because the sodium hydroxide treatment used in the Southern blot procedure would degrade the RNA. (wikibooks.org)
  • Generation of cell-free extracts of Xenopus eggs and demembranated sperm chromatin for the assembly and isolation of in vitro-formed nuclei for Western blotting and scanning electron microscopy (SEM). (antibodypedia.com)
  • Here, to characterize its biochemical properties, three separate antigenic regions of human CENP-C were expressed in Escherichia coli , affinity purified and used in South-western blotting and chemical cross-linking analyses. (springer.com)
  • The Northern blot is useful for the study of gene expression in two ways. (wikibooks.org)
  • The Northern blot is a valuable method used by researchers in determining gene expression patterns. (wikibooks.org)
  • Southern blotting confirmed that psaC gene had been successfully transformed and Tris-Tricine-PAGE revealed that psaC gene had been highly expressed after incubating the transformed strains in the inducing culture. (elsevier.com)
  • Using a south-western blot-based screen aimed at identifying factors that bind to plant histone gene promoters, we isolated a positive clone containing the tobacco LIM protein WLIM2 (NtWLIM2) cDNA.Using both GFP fusion- and immunology-based strategies, we provide clear evidence that NtWLIM2 localizes to the actin cytoskeleton, the nucleus and the nucleolus. (fnr.lu)
  • Summertime is over, but high outside temperature or not, you still need to defrost your samples for gels, Western blots and ELISAs. (advansta.com)
  • In this report, the United States Western Blot Imagers (Western Blot Analyzer) market is valued at USD XX million in 2016 and is expected to reach USD XX million by the end of 2022, growing at a CAGR of XX% between 2016 and 2022. (reportsnreports.com)
  • https://books.google.ca/books?id=_6cA03bXwhsC&pg=PA150&lpg=PA150&dq=ablan+leon+1942&source=bl&ots=38WoXc6EeK&sig=QytwEWFX1AD3HkJD5g35FfjBDus&hl=en&sa=X&ved=0ahUKEwi0_svPj57XAhVM4SYKHdw9A_EQ6AEIYDAN#v=onepage&q=ablan%20leon%201942&f=false There are plenty of dividend options beyond banks The Globe and Mail March 8, 2008 Leon's to take over eight Sears locations CTV News February 23, 2016 News, Mississauga. (wikipedia.org)