An episomally replicating vector binds to the nuclear matrix protein SAF-A in vivo. (1/28)
pEPI-1, a vector in which a chromosomal scaffold/matrix-attached region (S/MAR) is linked to the simian virus 40 origin of replication, is propagated episomally in CHO cells in the absence of the virally encoded large T-antigen and is stably maintained in the absence of selection pressure. It has been suggested that mitotic stability is provided by a specific interaction of this vector with components of the nuclear matrix. We studied the interactions of pEPI-1 by crosslinking with cis-diamminedichloroplatinum II, after which it is found to copurify with the nuclear matrix. In a south-western analysis, the vector shows exclusive binding to hnRNP-U/SAF-A, a multifunctional scaffold/matrix specific factor. Immunoprecipitation of the crosslinked DNA-protein complex demonstrates that pEPI-1 is bound to this protein in vivo. These data provide the first experimental evidence for the binding of an artificial episome to a nuclear matrix protein in vivo and the basis for understanding the mitotic stability of this novel vector class. (+info)Identification of different isoforms of eEF1A in the nuclear fraction of human T-lymphoblastic cancer cell line specifically binding to aptameric cytotoxic GT oligomers. (2/28)
GT oligomers, showing a dose-dependent cytotoxic effect on a variety of human cancer cell lines, but not on normal human lymphocytes, recognize and form complexes with nuclear proteins. By working with human T-lymphoblastic CCRF-CEM cells and by using MS and SouthWestern blotting, we identified eukaryotic elongation factor 1 alpha (eEF1A) as the main nuclear protein that specifically recognizes these oligonucleotides. Western blotting and supershift assays confirmed the nature of this protein and its involvement in forming a cytotoxicity-related complex (CRC). On the contrary, normal human lymphocytes did not show nuclear proteins able to produce CRC in a SouthWestern blot. Comparative bidimensional PAGE and Western-blotting analysis for eEF1A revealed the presence of a specific cluster of spots, focusing at more basic pH, in nuclear extracts of cancer cells but absent in those of normal lymphocytes. Moreover, a bidimensional PAGE SouthWestern blot demonstrated that cytotoxic GT oligomers selectively recognized the more basic eEF1A isoform expressed only in cancer cells. These results suggest the involvement of eEF1A, associated with the nuclear-enriched fraction, in the growth and maintenance of tumour cells, possibly modulated by post-translational processing of the polypeptide chain. (+info)MFP1 is a thylakoid-associated, nucleoid-binding protein with a coiled-coil structure. (3/28)
Plastid DNA, like bacterial and mitochondrial DNA, is organized into protein-DNA complexes called nucleoids. Plastid nucleoids are believed to be associated with the inner envelope in developing plastids and the thylakoid membranes in mature chloroplasts, but the mechanism for this re-localization is unknown. Here, we present the further characterization of the coiled-coil DNA-binding protein MFP1 as a protein associated with nucleoids and with the thylakoid membranes in mature chloroplasts. MFP1 is located in plastids in both suspension culture cells and leaves and is attached to the thylakoid membranes with its C-terminal DNA-binding domain oriented towards the stroma. It has a major DNA-binding activity in mature Arabidopsis chloroplasts and binds to all tested chloroplast DNA fragments without detectable sequence specificity. Its expression is tightly correlated with the accumulation of thylakoid membranes. Importantly, it is associated in vivo with nucleoids, suggesting a function for MFP1 at the interface between chloroplast nucleoids and the developing thylakoid membrane system. (+info)An interaction between a MYC protein and an EREBP protein is involved in transcriptional regulation of the rice Wx gene. (4/28)
We previously demonstrated that a 31-bp nucleotide sequence located upstream of the rice Wx gene played an important role in its expression. We further showed that this cis-acting regulator interacts with nuclear proteins extracted from developing rice endosperm. We used the 31-bp sequence as bait in a yeast one-hybrid system to isolate several cDNA clones from a rice cDNA expression library. One of these cDNAs encodes a MYC protein, designated OsBP-5, which is 335 amino acids long and contains a putative basic helix-loop-helix-ZIP DNA-binding domain. This domain exhibits 50% amino acid sequence identity with the R/B proteins that regulate the expression of genes involved in anthocyanin biosynthesis in plants. The results of electrophoretic mobility shift assays (EMSAs) and Southwestern gel blots indicate that this protein binds specifically to the CAACGTG motif within the 31-bp sequence. However, by itself, the OsBP-5 protein is unable to trans-activate a lacZ reporter gene controlled by the 31-bp sequence when tested in a yeast expression system. Interestingly, OsBP-5 can trans-activate this reporter gene when another protein, OsEBP-89, a member of the EREBP family of transcription factors, is present. Furthermore, in vitro pull-down experiments show that a protein isolated from developing rice endosperm interacts with the OsBP-5 protein, and Western blots confirm that the interacting protein is OsEBP-89. The formation of a supershift band in EMSAs also indicates that two proteins interact with each other. Interference of OsBP-5 gene expression by double-stranded RNA reduces the amylose content in mature seed of transgenic rice plants but has no visible effect on their phenotype. These results suggest that the OsBP-5 and OsEBP-89 proteins act synergistically, perhaps as a heterodimer, to regulate the transcription of the rice Wx gene. (+info)Identification of GAPDH as a protein target of the saframycin antiproliferative agents. (5/28)
Saframycin A (SafA) is a member of a class of natural products with potent antiproliferative effects in leukemia- and tumor-derived cells. This activity is frequently conjectured to derive from the ability of saframycins to covalently modify duplex DNA. We used a DNA-linked affinity purification technique to identify GAPDH as a protein target of DNA-small molecule adducts of several members of the saframycin class. Nuclear translocation of GAPDH occurs upon treatment of cancer cells with saframycins, and depletion of cellular GAPDH levels by small interfering RNA transfection confers drug resistance. Roeder and coworkers have recently suggested that GAPDH is a key transcriptional coactivator necessary for entry into S phase. Our data suggest that GAPDH is also capable of forming a ternary complex with saframycin-related compounds and DNA that induces a toxic response in cells. These studies implicate a previously unknown molecular mechanism of antiproliferative activity and, given that one member of the saframycin class has shown efficacy in cancer treatment, suggest that GAPDH may be a potential target for chemotherapeutic intervention. (+info)Borrelia turcica sp. nov., isolated from the hard tick Hyalomma aegyptium in Turkey. (6/28)
Previously, a novel, fast-growing spirochaete was isolated from the hard tick Hyalomma aegyptium, which infests tortoises (Testudo graeca), by using Barbour-Stoenner-Kelly (BSK) II medium; the tick samples were taken from the Istanbul area in northwestern Turkey [Guner et al. (2003). Microbiology 149, 2539-2544]. Here is presented a detailed characterization of the spirochaete. Electron microscopy revealed that strain IST7T is morphologically similar to other spirochaetes of the genus Borrelia and possesses 15 to 16 flagellae that emerge from both polar regions. PFGE analysis revealed the genome to comprise a linear chromosome of approximately 1 Mb; two large linear plasmids of approximately 145 and 140 kb, and several small plasmids ranging from 50 to 20 kb in size were also found. The 16S rRNA gene sequence of this Borrelia isolate exhibited 99.4 to 99.8 % identity with other strains isolated from H. aegyptium and less than 99 % similarity with those of other Borrelia species. A phylogenetic tree, generated from 16S rRNA gene sequences, demonstrated that the spirochaete isolates from H. aegyptium clustered together and branched off from both Lyme-disease-related and relapsing-fever-associated Borrelia species. A single copy of the rrs gene was detected in the genome of strain IST7T by Southern hybridization. DNA-DNA hybridization results showed that strain IST7T was distinct from Lyme-disease-related Borrelia, Borrelia burgdorferi and the relapsing-fever-associated species Borrelia hermsii. The G+C content of strain IST7T is 30.0 mol%. From these genetic features, a novel Borrelia species, Borrelia turcica sp. nov., is proposed; the type strain is IST7T (= JCM 11958T = DSM 16138T). (+info)Interaction of an approximately 40 kDa protein from regenerating rat liver with the -148 to -124 region of c-jun complexed with RLjunRP coincides with enhanced c-jun expression in proliferating rat liver. (7/28)
The c-jun belongs to the family of proto-oncogenes and encodes for the protein Jun, a component of transcription factor AP-1 involved in regulation of the expression of genes indispensable for cell proliferation and differentiation. While the role of c-jun in the regulation of such genes has been well examined, the regulation of c-jun in proliferating cells is not fully understood. We have earlier reported that the -148 to -124 region of c-jun is involved in the positive regulation of c-jun transcription, and interacts with a positive regulatory factor (rat liver jun regulatory protein; RLjunRP) present in rat liver. In this investigation, we report that this region is differentially recognized in proliferating liver as evidenced by the formation of a complex, different from that observed with normal liver extract. The new complex appears as early as 2 h after partial hepatectomy and its appearance coincides with the rise in c-jun mRNA levels after partial hepatectomy. In regenerating rat liver nuclear extract, an additional protein of approximately 40 kDa (rRLjunRP) interacts with a pre-existing dimer of RLjunRP complexed with the -148 to -124 region of c-jun to form a slow-migrating complex. rRLjunRP appears to pre-exist in the cytosol and translocate to the nucleus as indicated by the continued presence of the retarded complex in nuclear extract prepared from partially hepatectomized rats treated with cycloheximide. UV crosslinking studies, South-Western blot analysis, SDS/PAGE of affinity-purified factor(s), and 2D-PAGE analysis clearly demonstrate that the additional factor induced in response to growth stimulus is an approximately 40 kDa, that binds with the dimer of RLjunRP and enhances the c-jun transcription. (+info)A role for UV-light-induced c-Fos: Stimulation of nucleotide excision repair and protection against sustained JNK activation and apoptosis. (8/28)
UV light (UV-C) is a potent inducer of the c-fos gene. Cells lacking c-Fos are hypersensitive to the cytotoxic effect of UV-C indicating a protective role of c-fos induction. Here we show that cells deficient in c-Fos (fos-/-) are unable to remove cyclobutane pyrimidine dimers (CPDs) from DNA and undergo apoptosis at high frequency via the Fas pathway. We also show that in c-Fos-deficient cells the activation of c-Jun N-terminal kinase (JNK) by UV-C differs from the wild-type (wt, fos+/+). In wt cells JNK activation is transient, returning to control level 5-8 h after treatment, whereas in c-Fos-deficient cells JNK activation was long-lasting and did not return to control level. Inhibition of early JNK activation by the JNK inhibitor SP600125 attenuated CPD repair and increased UV-C induced apoptosis whereas inhibition of late JNK activation attenuated the apoptotic response of c-Fos-deficient cells. Late and sustained activation of JNK resulted in upregulation of fas (CD95, apo-1) ligand and induction of caspase 8-dependent apoptosis. The data indicate that the immediate-early induction of the JNK/c-fos pathway stimulates the repair of UV-C induced DNA lesions that protects against apoptosis. If this does not occur, cells do not recover from transcription blockage leading, as shown for c-Fos-deficient cells, to a reduced expression of MKP1, sustained JNK activation and fasL overexpression that finally results in activation of the death receptor pathway. The data support the hypothesis that non-repaired DNA damage is the cause for the late and sustained activation of the MAP kinase pathway in response to genotoxins. (+info)
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Southwestern blot
Thereafter southwestern blotting begins to vary with regards to procedure as since the first blotting's, many more have been ... "Southwestern blot mapping" is a time-efficient way of identifying DNA-binding proteins and specific sites on the genomic DNA ... Southwestern+Blot at the US National Library of Medicine Medical Subject Headings (MeSH) Bowen, B; Steinberg, J; Laemmli, U K; ... The southwestern blot, is a lab technique that involves identifying as well as characterizing DNA-binding proteins by their ...
Eastern blot
The method is similar to southwestern blot. (2006) Eastern blotting has been used to refer to the detection of fusion proteins ... Western blot Northwestern blot Far-eastern blot Blot Freeze, HH (1993). "Preparation and analysis of glycoconjugates". Current ... The method is similar to southwestern blot. (2011) A recent study used the term eastern blotting to describe detection of ... Since this is essentially a western blot, the charge reversal was used to dub this method an eastern blot. (2005) Eastern blot ...
Northwestern blot
Southern blot Western blot Northern blot Southwestern blot Eastern blot Gel electrophoresis SDS-PAGE Chromatography ... These techniques include the western blot (protein detection), the northern blot (RNA detection), the southwestern blot (DNA- ... C, Franke; Grafe D; Bartsch H; Bachmann M (2009). Use of non-radioactive detection method for north- and southwestern blot. ... The northwestern blot allows for detection of identified proteins in a way that is inexpensive. The blot is typically a first ...
Dot blot
Southern blot Northern blot Western blot Southwestern blot "Test Blots, Slot Blots & Dot Blots - Immunodetection , Bio-Rad". ... Performing a dot blot is similar in idea to performing a western blot, with the advantage of faster speed and lower cost. Dot ... A dot blot (or slot blot) is a technique in molecular biology used to detect proteins. It represents a simplification of the ... A general dot blot protocol involves spotting 1-2 microliters of a samples onto a nitrocellulose or PVDF membrane and letting ...
Southern blot
Other blotting methods (i.e., western blot, northern blot, eastern blot, southwestern blot) that employ similar principles, but ... acids Restriction fragment Genetic fingerprint Northern blot Western blot Eastern blot Southwestern blot Northwestern blot " ... Southern blotting transfer may be used for homology-based cloning on the basis of amino acid sequence of the protein product of ... A Southern blot is a method used in molecular biology for detection of a specific DNA sequence in DNA samples. Southern ...
Blot (biology)
... making the Far-Western blot a specific case of the Western blot. A southwestern blot is based on Southern blot and is used to ... Southern blot for DNA northern blot for RNA reverse northern blot for RNA western blot for proteins far-western blot for ... A dot blot is a special case of any of the above blots where the analyte is added directly to the blotting matrix (and appears ... The reverse northern blot differs from both northern and Southern blot in that DNA is first immobilized on a blotting matrix ...
Outline of biochemistry
... dynamics Mass spectrometry Isotopic labeling Coimmunoprecipitation Electrophoretic mobility shift assay Southwestern blotting ... reaction Northern blotting Southern blotting Fusion proteins DNA microarray Bioinformatics Flow cytometry Western blotting ... Southern blot - fractionation - Gram stain - Surface Plasmon Resonance - Microscale Thermophoresis DNA sequencing Polymerase ...
Index of molecular biology articles
... slot blot - SNP - Slc22a21 - snRNA - snRNP - solution hybridization - somatic cells - Southern blot - southwestern blot - SP6 ... blotting - blunt end - bone marrow transplantation - box - BP - BRCA1 - BRCA2 - Brix (database) - C terminus - Can f 1 - cancer ... Western blot - WHEP-TRS protein domain - wildtype - wobble position - Wolfram syndrome - WWE protein domain - XPG I protein ... dot blot - double helix - downstream (DNA) - downstream (transduction) - ds - duplex - E. coli - Ecotin - EIF-W2 protein domain ...
2022 Hunga Tonga-Hunga Ha'apai eruption and tsunami
Ash from the eruption made landfall on the main island of Tongatapu, blotting out the sun. Loud explosions were heard 65 km (40 ... Tremors and shaking buildings were reported by residents in southwestern Niue, around Alofi and Avatele. The United States ...
List of pagans
... king of Sweden Blot-Sweyn, leader of the Swedish pagan renaissance in the 11th century Coifi, priest of the temple at ... in south-eastern Norway and south-western Sweden He is portrayed in Snorri Sturluson's saga Heimskringla. Gibuld (fl. c. 470), ... semi-historical successor to Blot-Sweyn, and the last pagan king in Scandinavia Erik Ringsson, Swedish king and the son of Ring ...
Cape Smyth
Other locations named Cape Smyth are: (1) the south-western point of Melville Island, between Warrington Bay and Hardy Bay, in ... https://books.google.co.uk/books?id=0y48AQAAMAAJ&pg=PA108&lpg=PA108&dq=cape+smyth+point+barrow&source=bl&ots=AUymog872d&sig= ...
Wayne H. Holtzman
"Holtzman Ink Blot Test". The Free Dictionary. Archived from the original on 3 August 2020. Retrieved 18 April 2021. " ... "History". Southwestern Psychological Association. Archived from the original on 8 February 2020. Retrieved 27 March 2021. "Ten ... In 1959 he was elected president of the Southwestern Psychological Association and was promoted to professor by the University ...
Americus, Georgia
Georgia Southwestern State University Archived 2010-07-06 at the Wayback Machine, Retrieved June 26, 2010. South Georgia ... 73-74https://books.google.com/books?id=svf_7DV9i6UC&pg=PA129&lpg=PA129&dq=remembering+americus+georgia+essays&source=bl&ots= ... Americus is the home of Habitat for Humanity's international headquarters, Georgia Southwestern State University, the Windsor ... now Georgia Southwestern State University), and the South Georgia Trade and Vocational School in 1948 (now South Georgia ...
Carya pallida
Symptoms include fading light-green blots on the adaxial side and floury, white, furry blots on the abaxial side. Other hickory ... Rarely, it can be found in southwestern Indiana, and it is uncommon in Maryland. Population decline has been reported in ...
Tables game
Instead, the attacking player may pin a blot by moving a piece onto the same point. The blot is not removed from the board, but ... from its invention or earliest appearance in Southwestern Asia (or Persia) before AD 800 Tables period: tables games from their ... The blot is 'hit' or 'knocked off' the board and is usually placed on the bar between the two halves of the board. Pinning ... Zeno, who was white, had a stack of seven men, three stacks of two men and two blots, men that stood alone on a point and were ...
Buocher Höhe
The Buocher Höhe is usually seen as the area on the southwestern edge of the forested upland of Berglen, which is sometimes ... Below this thin layer lies Rhätsandstein, which was formerly milled into blotting sand for the drying of ink. The old quarry ...
Farah Hussein Sharmarke
Farah was born in 1926 in the city of Bardera in Gedoregion in south western Somalia. He studied poetry from other famous Gedo ... bl&ots=R3oVuMa07c&sig=zK55RhDiHiyJEOUQzxQQNX_VHn0&hl=en&sa=X&ei=_qmZVJ6jH4KvoQSgkoHABQ&ved=0CDMQ6AEwBA#v=onepage&q=farah% ...
Duck circovirus
The PCR and dot blot hybridization (DBH) test was used in 2006-2007. 742 ducks from 70 duck farms were tested. The overall ... "Complete Genome Sequence of the Novel Duck Circovirus Strain GH01 from Southwestern China". Genome Announcements. 1 (1): e00166 ... Scientists have studied Duck circovirus by using polymerase chain reaction (PCR)-based methods and dot blot hybridisation (DBH ...
Gao Huan
He saw Emperor Xiaowu's flight as a blot on his personal history, so he treated Emperor Xiaojing with great formal respect, ... In counterattacks, Western Wei took modern western Henan and southwestern Shanxi. In winter 537, after hearing news that the ...
List of MeSH codes (E05)
... blotting, northern MeSH E05.393.525.150 - blotting, southern MeSH E05.393.525.225 - blotting, southwestern MeSH E05.393.525.300 ... blotting, northern MeSH E05.196.181.400.454.655.150 - blotting, southern MeSH E05.196.181.400.454.720 - peptide mapping MeSH ... blotting, northern MeSH E05.196.401.319.670.150 - blotting, southern MeSH E05.196.401.319.720 - peptide mapping MeSH E05.196. ... blotting, western MeSH E05.478.567.320.200.200 - blotting, far-western MeSH E05.478.567.350 - immunoenzyme techniques MeSH ...
Blood Meridian
It was his first attempt at a western and his first novel set in the Southwestern United States, a change from the Appalachian ... He believes there is no reason to "blot the page up with weird little marks". The New York Times described McCarthy's prose in ... Southwestern Writers Collection Series. Foreword by Edwin T. Arnold (Revised and Expanded ed.). University of Texas Press. ISBN ...
Henie Onstad Kunstsenter
Blot, Paris, Coll. De la Chapelle, Paris, Gal, Bénézit, Paris Pablo Picasso, Femme assise dans un fauteil, 1941 Provenance: Gal ... southwestern France. The Einsatzstab Reichsleiter Rosenberg entered the vault in March 1941 to confiscate the art pieces, and ...
Chronic wasting disease
In May 2001, CWD was also found in free-ranging deer in the southwestern corner of Nebraska (adjacent to Colorado and Wyoming) ... The brainstem tissue, but not lymph nodes, was positive for CWD (confirmed with Western Blot). A second case of CWD was ... The area in 2006 has expanded to six states, including parts of eastern Utah, southwestern South Dakota, and northwestern ... western blotting (WB), enzyme immunoassay (EIA), protein misfolding cyclic amplification (PMCA), and real time quaking-induced ...
Koala conservation
Subsequently, 63% of the population in southwestern Queensland died, especially young animals that were excluded from prime ... And he has been almost blotted out already from some areas.: 127 Despite the growing movement to protect native species, the ...
Trypanosoma cruzi
It was indicated through Western blot that cyclin D1 was upregulated from day 1 to day 60 post-infection. It was also indicated ... T. cruzi transmission has been documented in the Southwestern U.S., and warming trends may allow vector species to move north. ...
Schwarzenberg, Saxony
The city issued 42 postage stamps in 1945, all showing a heavy black silhouetted outline of the city blotting out Hitler's face ... Schwarzenberg is in the southwestern Ore Mountains. It lies at elevations stretching from 428.5 to 823 metres (1,406 to 2,700 ...
Knockentiber
... blotting out' the information on a milestone which could not be buried easily; the fate of so many other milestones. Another ... then as part of the Glasgow and South Western Railway) on 1 September 1872.and closed permanently to passengers on 6 April 1964 ...
Dow Crag
The south western branch traverses to Dow Crag and the south eastern to The Old Man of Coniston, with Goat's Water lying in a ... The view south and west to the coast is excellent, but much of Lakeland is blotted out by the flanks of the Coniston range. The ...
Delphinium scopulorum
It is native to the Southwestern United States and found predominantly in upper-elevation moist meadows. D. scopulorum is a non ... bl&ots=R__340kIoL&sig=vSmByyCCaGdIptZ278KseOsL6hw&hl=en&ei=gsrTTZiECYTfgQeXgb0u&sa=X&oi=book_result&ct=result&resnum=1&ved= ...
Doha
... h stands on the south side of a deep bay at the south-western corner of a natural harbor which is about 3 miles in extent ... A British record later stated "that the towns of Doha and Wakrah were, at the end of 1867 temporarily blotted out of existence ... relating to Aden and the south western coast of Arabia, the Arab principalities in the Persian Gulf, Muscat (Oman), Baluchistan ...
Franklin, North Carolina
Cullasaja Falls is a waterfall in Southwestern North Carolina. The waterfall is located on the Cullasaja River in the Nantahala ... id=tDVWkTVnYwQC&pg=PA278&lpg=PA278&dq=%22Charles+Frazier%22+%22franklin,+north+carolina%22&source=bl&ots=WPGlvBRBqR&sig= ...
Petunioideae
Hunzikeria D'Arcy 3 species from the southwestern United States and Mexico. Leptoglossis Benth. 7 species from western South ... bl&ots=tLt8RWPMU4&sig=ACfU3U0CoKR2hc8ymlfJYJsCoQPTbD0ovg&hl=en&sa=X&ved=2ahUKEwja14DfqIDhAhWgTxUIHROBBmMQ6AEwHnoECAkQAQ#v= ...
Michael Rockefeller
He disappeared during an expedition in the Asmat region of southwestern Netherlands New Guinea, which is now a part of the ... bl&ots=0nWxxcCC_q&sig=Bt1zD_9PLGiFxKmX447HUrsaN68&hl=en&ei=GdusS4H6DYSBlAfNpojUDQ&sa=X&oi=book_result&ct=result&resnum=3&ved= ...
Lascaux
"Lectures on the Ice-Age Painted Caves of Southwestern France" (PDF). Ferrier, Catherine; Debard, Évelyne; Kervazo, Bertrand; ... that was swabbed or blotted on, rather than applied by brush. In other areas, the colour was applied by spraying the pigments ... Dickson, D. Bruce (1992). The Dawn of Belief: Religion in the Upper Paleolithic of Southwestern Europe. ISBN 9780816513369. ... in the department of Dordogne in southwestern France. Over 600 parietal wall paintings cover the interior walls and ceilings of ...
Wood Islands, Prince Edward Island
The southwestern shore of the lagoon forming Wood Islands Harbour is also the southernmost point in Prince Edward Island with ... bl&ots=5xAnztEpXU&sig=UdG3W6AnQI5IuhfaknGNgtmz3sA&hl=en&sa=X&ved=0ahUKEwjt4rGu_Y7YAhVD4oMKHXOeARsQ6AEITzAH#v=onepage&q=Burnt% ...
Cabo de Gata-Níjar Natural Park
Although the villages or "ghost towns" and the former gold mines are a blot on the landscape, they are a big attraction. Sadly ... It is the only palm species native to continental Europe and is mainly found in southwestern Europe (Spain, Portugal, Italy, ...
Red-winged blackbird
The wing blot becomes bright scarlet-vermilion, while deep ocher beige coloration appears on the medium coats. Other sources ... In southwestern Michigan, the density of cattail stems in different ponds was positively related to the concentration of ... A. p. nyaritensis - coastal plains of southwestern Mexico (Nayarit). A. p. grinnelli - Pacific Slope from western Guatemala to ... On the other hand, in southwestern Quebec, red-winged blackbird populations doubled between 1966 and 1981, apparently in ...
Methicillin-resistant Staphylococcus aureus
Blot SI, Vandewoude KH, Hoste EA, Colardyn FA (October 2002). "Outcome and attributable mortality in critically Ill patients ... rural southwestern Alaska". Emerging Infectious Diseases. 14 (11): 1693-9. doi:10.3201/eid1411.080381. PMC 2630737. PMID ... Several studies, however, including one by Blot and colleagues, that have adjusted for underlying disease still found MRSA ...
Battle of Pease River
With an oncoming blue northern blotting out sign, Ross was able to move up to spy out the location of the Noconi on the Mule ... The Southwestern Historical Quarterly, 113(1), 32-52. Indian Depredations in Texas by J.W. Wilbarger, at archive.org Biography ...
Christopher Csíkszentmihályi
... an Unmanned aerial vehicle designed to observe militia activity in the Southwestern United States RoBoat (2006), a robotic ... p507 https://books.google.com/books?id=tqW75bfJkxIC&pg=PA507&lpg=PA507&dq=%22dj+i,+robot%22&source=bl&ots=SmZSTLKJVb&sig=ngwBl_ ...
South Eastern Railway (England)
Both the LB&SCR and London and South Western Railway (L&SWR) regarded this line as a significant incursion into their areas of ... Ernest Foxwell, also writing in 1883, stated 'The great blots on the South Eastern are its unpunctuality, its fares, its third ...
Battle of Monte Cassino
The dark rain clouds also blotted out the moonlight, hindering the task of clearing routes through the ruins. On the right, the ... and responsible for the Gustav defences on the south western half of the line, some serious concern as to the ability of the ... Torrents of rain flooded bomb craters, turned rubble into a morass and blotted out communications, the radio sets being ...
History of the American legal profession
By the early 21st century, in the southwestern states from Texas to California, well-educated Latinas confronted the triple ... bl&ots=Rf6APTXWQe&sig=ACfU3U1CTYtUoqKcUCn65hXwwHiGuvKXjw&hl=en&sa=X&ved=2ahUKEwi64POQmaTqAhVC-qQKHZ-qAyMQ6AEwAXoECAYQAQ#v= ... bl&ots=u3vlM-ruxW&sig=ACfU3U2ZxLrm1v44ugHL05fZQT9rKeeXhQ&hl=en&sa=X&ved=2ahUKEwjYloX7mqTqAhXF0aQKHfDIDToQ6AEwBXoECAkQAQ#v= ... bl&ots=CHu5t72Zvo&sig=ACfU3U18iCbXT1L1LwLXlJbFfcBxcIWzZw&hl=en&sa=X&ved=2ahUKEwizpa3AmqTqAhVmMOwKHReiDYoQ6AEwAHoECAoQAQ#v= ...
Scientific misconduct
Harold Garner's laboratory at the University of Texas Southwestern Medical Center at Dallas is Déjà vu, an open-access database ... information is not visible Image manipulations are typically done on visually repetitive images such as those of blots and ...
Al Bidda
A British record later stated "that the towns of Doha and Wakrah were, at the end of 1867 temporarily blotted out of existence ... relating to Aden and the south western coast of Arabia, the Arab principalities in the Persian Gulf, Muscat (Oman), Baluchistan ...
Frank Pick
One day the ice will descend and blot out all traces of man's works. The earth will grow cold. Nature will return to her ... Pick still faced strong opposition from the London, Brighton and South Coast Railway and the London and South Western Railway ...
List of German Americans
... bl&ots=FLSn_wp-GL&sig=Xiro1qxm5uWy1xDBtT_g3lAkUlY&hl=en&sa=X&ved=0ahUKEwiZ4NKe78XTAhXDLyYKHYtWANYQ6AEILzAD#v=onepage&q=john% ... southwestern Germany." http://www.ancestry.com/name-origin?surname=keck "German: nickname from Middle High German kec 'lively ... bl&ots=n5B6Q58Wwl&sig=mg4vDQfFqF8ChIgWGLbDwPV3oEE&hl=en&sa=X&ved=0ahUKEwjksu3i0_fJAhUIVyYKHagwDqsQ6AEIKTAC#v=onepage&q=Nesser% ... bl&ots=4qpnO2YsoT&sig=ACfU3U0pDqS_mtxPpXHsXlin6etGZ-w68w&hl=en&sa=X&ved=2ahUKEwighMr-y8jiAhXhRt8KHTHzDYoQ6AEwB3oECAkQAQ#v= ...
History of Qatar
A British record later stated: "(...) the towns of Doha and Wakrah were, at the end of 1867 temporarily blotted out of ... relating to Aden and the south western coast of Arabia, the Arab principalities in the Persian Gulf, Muscat (Oman), Baluchistan ...
Jacques Chirac
His great-grandparents on both sides were peasants in the rural south-western region of the Corrèze. According to Chirac, his ... He appointed Yvan Blot, an intellectual who would later join the National Front, as director of his campaigns for the 1979 ...
Harvard Australian Expedition (1931-1932)
Operating mostly in eastern and southwestern Australia, at least four of the species they collected "were new to science". ... bl&ots=z7sMfkRcr3&sig=F82aRX9ZWuNqujfCXBHj90YFs5A&hl=en&sa=X&ved=0ahUKEwjt3Kq1_K3bAhWB7lMKHW0uAJ8Q6AEIWTAF#v=onepage&q=Harvard% ...
Rudolf Vrba
... trying to blot him out ... and Yankel, who had forgotten to sew his buttons on, had not even the good grace to die quickly and ... in southwestern Poland that transcend the horrors of Lublin." Cotler wrote to Gilbert on 18 February 1992: "I fully concur with ...
Parabolic arch
... and the beehive huts of southwestern Ireland. In the modern period, parabolic arches were first used extensively from the 1880s ... "+"parabolic+arch"&source=bl&ots=uOzbRBeDwm&sig=ohyUSTlHvU8sNUqTahR_BjU6gp4&hl=en&sa=X&redir_esc=y#v=onepage&q="hen's egg" " ...
Association of novel POLG mutations and multiple mitochondrial DNA deletions with variable clinical phenotypes in a Spanish...
Capel Gava 3495-440 Rugs | Southwestern Wool Rugs | Rugs Direct
Sonora Flatweave Jute Rug | Mitchell Gold + Bob Williams
A Classic Southwestern geometric diamond pattern gets the modern treatment with overdyed yarns and a contrasting natural jute ... Blot any spill immediately, use mild cleaners that are synthetic fiber safe ... Modern treatment to classic Southwestern geometric diamond pattern. *Vacuum regularly with a low-power setting without a beater ...
Southern, Northern, Western, and Eastern blots | American Association for the Advancement of Science (AAAS)
Far-Eastern blots, Middle Eastern blots, Southwestern blots etc. that will not be described here. ... Molecular biologists being punny fellows, this was immediately dubbed the Northern blot. Northern blots were invaluable for ... Like Southerns, Western blots are also used for diagnostic assays, for instance, the confirmation of HIV infection. ... Now, of course, the Southern blot has also been adapted for forensic DNA analysis. A number of prisoners have been released ...
Watch Desperate Housewives Streaming Online | Hulu (Free Trial)
Jaipur Living Anatolia Blue Rectangle 5x8 ft Wool Carpet 62691 | SKU 62691
MH DELETED MN ADDED MN
Shang, Z. F.<...
DeCS
Blot, Southwestern. Blots, Southwestern. Blottings, Southwestern. Southwestern Blot. Southwestern Blots. Southwestern Blotting ... Blottings, Southwestern Southwestern Blotting Southwestern Blottings Blot, Southwestern - Related but not broader or narrower ... Blotting, Southwestern - Preferred Concept UI. M0378350. Scope note. A method that is used to detect DNA-protein interactions. ... Proteins are separated by electrophoresis and blotted onto a nitrocellulose membrane similar to Western blotting (BLOTTING, ...
SMART: ZnF C2HC domain annotation
ZURICH OLIVE Southwestern Velvet Upholstery Fabric
Distributor prices on ZURICH OLIVE southwestern velvet upholstery fabric. Decorative Fabrics Direct since 1947, fabric and ... Most stains can be blotted and cleaned with just water, but we recommend mixing some bleach in the solution to kill any ... Decrease Quantity of 6409211 ZURICH OLIVE Southwestern Velvet Upholstery Fabric Increase Quantity of 6409211 ZURICH OLIVE ... ZURICH OLIVE Southwestern Velvet Upholstery Fabric. Zurich Olive is a jacquard woven upholstery velvet with a boucle ground ...
Southwestern Campos Turkish Hand-Woven Kilim Pillow - 18'' x 18'' - Bareens Designer Rugs
Embellish your home with this timeless casual chic handmade Southwestern Kilim throw pillow covers made with wool and cotton. ... Clean spills immediately; if liquid, blot with clean, undyed cloth by pressing firmly around the spill to absorb as much as ... Home Southwestern Campos Turkish Hand-Woven Kilim Pillow - 18 x 18 .halo-product-content .pro-page { padding-top: 10px; } @ ... Embellish your home with this timeless casual chic handmade Southwestern Kilim throw pillow covers made with wool and cotton. ...
8x10 Hand Knotted Red and White Modern Contemporary Southwestern Triba
- The Rug Decor
Blot spills immediately with a white cloth. Harsh chemicals may damage or fade the rug ... 8x10 Hand Knotted Red and White Modern Contemporary Southwestern Tribal Trellis Recycled Silk Area Rug , TRDOP2 ... 8x10 Hand Knotted Red and White Modern Contemporary Southwestern Tribal Trellis Recycled Silk Area Rug , TRDOP2 ... 8x10 Hand Knotted Red and White Modern Contemporary Southwestern Tribal Trellis Recycled Silk Area Rug , TRDOP2 ...
The Methods January 2000 Archive by subject
Southwestern blot??? Lluís Armengol *Southwestern Protocol Sandra Pena *Stable puromycin cell line query!! Csaba Kiss * ... Western Blotting Problem R. John Lye *Western Blotting Problem Richard P. Grant *Western Blotting Problem Bernard Murray, PhD * ... blotting of large RNAs Frederik Börnke *blotting of large RNAs Nick Theodorakis *Book on mouse ES cells Jose E. Mejia *BSA ... Fundamental Northern Blot question Frank O. Fackelmayer *Fundamental Northern Blot question JEJS Jan Eric Jensen *Funding for ...
Preventing Chronic Disease | Cancer Disparities Among Alaska Native People, 1970-2011 - CDC
Blot WJ, Lanier AP, Fraumeni JF Jr, Bender TR. Cancer mortality among Alaskan natives, 1960-69. J Natl Cancer Inst 1975;55(3): ... Fortuine R. Characteristics of cancer in the Eskimos of southwestern Alaska. Cancer 1969;23(2):468-74. CrossRef PubMed ... Lanier AP, Bender TR, Blot WJ, Fraumeni JF Jr, Hurlbert WB. Cancer incidence in Alaska natives. Int J Cancer 1976;18(4):409-12 ...
All About Lembar & Southwestern Peninsula ~ Jelajah Indonesiaku
Search | African Index Medicus (AIM)
Seroprevalence of toxoplasma gondii and neospora caninum in urban and rural dogs from southwestern Nigeria ... Nigeria and tested by the western blotting technique for specific IgG to T. gondii and N. caninum.Results: The seroprevalence ... This study investigated the prevalence of antibodies to Toxoplasma gondii and Neospora caninum in dogs from southwestern ... gondii antibodies in dogs in southwestern Nigeria ...
18 preachers, 12 songleaders and one God - The Christian Chronicle
MeSH Browser
8 x 10 Large Arrowhead Light Blue and Beige Area Rug - Adagio | RC Willey
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MH DELETED MN ADDED MN
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Evaluation of gene targeting by homologous recombination in ovine somatic cells. - Department of Oncology
Búsqueda | Portal Regional de la BVS
Spills immediately4
- Clean spills immediately by blotting with a cloth or sponge. (rugs-direct.com)
- Blot spills immediately with a white cloth. (therugdecor.com)
- Blot spills immediately. (rugs.shop)
- Blot spills immediately by pressing firmly with clean, dry, white paper towels or cloth towels, working from the outside of the spill inward to avoid spreading. (rugandhome.com)
Geometric1
- A Classic Southwestern geometric diamond pattern gets the modern treatment with overdyed yarns and a contrasting natural jute fringe accent. (mgbwhome.com)
Stains2
- Blot stains immediately and clean with mild soap mixed with equal parts white vinegar and water. (rugman.com)
- Never scrub at spills or stains, but instead use a blotting motion with a white or light-colored cloth to prevent dye transfer. (rugs-direct.com)
Cloth4
- if liquid, blot with clean, undyed cloth by pressing firmly around the spill to absorb as much as possible. (bareens.com)
- If it is a liquid spill, blot with clean cloth around the spill to absorb as much as possible. (overstock.ca)
- For spills, remove excess and blot with a clean cloth dampened with lukewarm water. (wellwoven.com)
- Finish by Blotting with water and a clean white cloth. (wellwoven.com)
Membrane3
- The DNA was first treated with restriction enzymes to make the fragments, then subjected to agarose gel electrophoresis to separate them by size, then denatured in situ and transferred to a nitrocellulose membrane by virtue of sucking volumes of buffer through the gel and the membrane, using stacks of paper towels to blot up the buffer. (aaas.org)
- Many different techniques have been variously described as Eastern blotting, most of which involve using different types of probes to detect post-translational modifications of proteins, for instance the use of lectins to probe a protein-blotted membrane in search of specific carbohydrates. (aaas.org)
- Proteins are separated by electrophoresis and blotted onto a nitrocellulose membrane similar to Western blotting (BLOTTING, WESTERN) but the proteins are identified when they bind labeled DNA PROBES (as with Southern blotting (BLOTTING, SOUTHERN)) instead of antibodies. (bvsalud.org)
Wool1
- Embellish your home with this timeless casual chic handmade Southwestern Kilim throw pillow covers made with wool and cotton. (bareens.com)
Chic1
- A kilim works well with many home decor styles, including Mid-Century Modern , industrial chic, Southwestern, and transitional . (newstimes.com)
Immediately1
- Molecular biologists being punny fellows, this was immediately dubbed the Northern blot. (aaas.org)
Rugs2
Assays2
- Like Southerns, Western blots are also used for diagnostic assays, for instance, the confirmation of HIV infection. (aaas.org)
- Traditional western blot evaluation and immunoprecipitation assays Isolation of entire cell lysates and cytoplasmic and nuclear ingredients had been performed as defined previously (20). (irpa2006europe.com)
Pattern1
- This indoor rug is perfect for those traditional terracotta aesthetics with its deep earth tones and intricate southwestern lodge pattern. (yahoo.com)
Clean1
- Blot well on paper towels or clean tea towels, then cut into small dice. (vegkitchen.com)
Western8
- The pun was stretched even further to include Western blots, also developed in the Stark laboratory. (aaas.org)
- There is a whole menagerie of variations on the Southern's original theme: Far-Western blots, Far-Eastern blots, Middle Eastern blots, Southwestern blots etc. that will not be described here. (aaas.org)
- Se separan las proteínas por electroforesis y se secan en una membrana de nitrocelulosa similar a la utilizada en el método Western (TÉCNICA WESTERN) pero se identifican las proteínas cuando se unen a SONDAS DE ADN etiquetadas (como con la TRANSFERENCIA DE SOUTHERN) en lugar de anticuerpos. (bvsalud.org)
- Western blot analysis for beta2-transferrin allows identification with very small amounts of dilute fluid. (medscape.com)
- Preoperative irrigation of the middle ear via myringotomy has been suggested as a method of obtaining dilute perilymph that could be tested by Western blot analysis for the presence of beta2-transferrin, thus providing preoperative evidence of perilymphatic fistula (PLF). (medscape.com)
- Additionally, sensitivity of Western blot analysis for beta2-transferrin has been demonstrated to be very low (29%) in one study testing specimens of known perilymph. (medscape.com)
- You look at the results of your Western blot and you can tell that the levels of your protein are changing. (advansta.com)
- Do I Capitalize Western Blot? (advansta.com)
Style1
- You may want to add the color of your space or to add a touch of southwestern style, a southwestern rug is the ideal solution to do it. (b-cdn.net)
Vacuum1
- Rotate and gently vacuum your rug, keep it out of direct sunlight, blot spills right away, and use a pad underneath to avoid slipping and creasing," Markley says. (newstimes.com)
Durable1
- It's durable enough to be stepped on for years without matting or wearing down, and spills are easy to blot out. (yahoo.com)
Remove1
- Scoop up solids and blot liquids to remove as much of the offending substance as possible. (rcwilley.com)
Paper1
- Usually has Japanese blotting paper, OPI Shatter nail polish, Skin79 BB Cream, Hanskin Super Magic BB Cream, KMS California and A.C. Care products. (visitkoreatown.org)
Traditional2
- split --\u003e\n\u003cp\u003eThe Tulsa collection draws upon a curated array of traditional Persian, Southwestern, and Moroccan designs. (wellwoven.com)
- The Tulsa collection draws upon a curated array of traditional Persian, Southwestern, and Moroccan designs. (wellwoven.com)
Analysis2
White1
- It is so important that we let the world and especially our children see that there is neither black or white or Hispanic but that we are all one in Christ Jesus," said Bradshaw, an alumnus of both Southwestern Christian College and Abilene Christian University. (christianchronicle.org)
Term1
- As the only direction remaining, the term "Eastern blotting" has been fought over extensively. (aaas.org)
University2
- John D. Minna, Hamon Center for Therapeutic Oncology Research NB8.206, The University of Texas Southwestern Medical Center at Dallas, 6000 Harry Hines Boulevard, Dallas, TX 75390-8593. (aacrjournals.org)
- Surgeons at the University of Texas Southwestern Medical Center make a small incision beneath the lobule from which parotid masseteric fascia is harvested for use as a graft. (medscape.com)
Continue1
- Continue blotting until no moisture transfers to the towel. (rugandhome.com)
Sizes1
- There are many sizes available to help you find the best Southwestern rug for your home. (b-cdn.net)
Specific1
- Northern blots were invaluable for detecting and quantifying specific mRNAs, thus determining the activity of different genes. (aaas.org)
Designs1
- Southwestern rug designs can be used to add a touch of fashion and color to any space inside your home. (b-cdn.net)
Free1
- Blotbase , from medicalgenomics.org, is a free database of mRNA sequences quantified by Northern blots from various cell types and tissues. (aaas.org)