Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
A method that is used to detect DNA-protein interactions. Proteins are separated by electrophoresis and blotted onto a nitrocellulose membrane similar to Western blotting (BLOTTING, WESTERN) but the proteins are identified when they bind labeled DNA PROBES (as with Southern blotting (BLOTTING, SOUTHERN)) instead of antibodies.
Synthetic or natural oligonucleotides used in hybridization studies in order to identify and study specific nucleic acid fragments, e.g., DNA segments near or within a specific gene locus or gene. The probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the probe include the radioisotope labels 32P and 125I and the chemical label biotin.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A nitrocellulose solution in ether and alcohol. Collodion has a wide range of uses in industry including applications in the manufacture of photographic film, in fibers, in lacquers, and in engraving and lithography. In medicine it is used as a drug solvent and a wound sealant.

An episomally replicating vector binds to the nuclear matrix protein SAF-A in vivo. (1/28)

pEPI-1, a vector in which a chromosomal scaffold/matrix-attached region (S/MAR) is linked to the simian virus 40 origin of replication, is propagated episomally in CHO cells in the absence of the virally encoded large T-antigen and is stably maintained in the absence of selection pressure. It has been suggested that mitotic stability is provided by a specific interaction of this vector with components of the nuclear matrix. We studied the interactions of pEPI-1 by crosslinking with cis-diamminedichloroplatinum II, after which it is found to copurify with the nuclear matrix. In a south-western analysis, the vector shows exclusive binding to hnRNP-U/SAF-A, a multifunctional scaffold/matrix specific factor. Immunoprecipitation of the crosslinked DNA-protein complex demonstrates that pEPI-1 is bound to this protein in vivo. These data provide the first experimental evidence for the binding of an artificial episome to a nuclear matrix protein in vivo and the basis for understanding the mitotic stability of this novel vector class.  (+info)

Identification of different isoforms of eEF1A in the nuclear fraction of human T-lymphoblastic cancer cell line specifically binding to aptameric cytotoxic GT oligomers. (2/28)

GT oligomers, showing a dose-dependent cytotoxic effect on a variety of human cancer cell lines, but not on normal human lymphocytes, recognize and form complexes with nuclear proteins. By working with human T-lymphoblastic CCRF-CEM cells and by using MS and SouthWestern blotting, we identified eukaryotic elongation factor 1 alpha (eEF1A) as the main nuclear protein that specifically recognizes these oligonucleotides. Western blotting and supershift assays confirmed the nature of this protein and its involvement in forming a cytotoxicity-related complex (CRC). On the contrary, normal human lymphocytes did not show nuclear proteins able to produce CRC in a SouthWestern blot. Comparative bidimensional PAGE and Western-blotting analysis for eEF1A revealed the presence of a specific cluster of spots, focusing at more basic pH, in nuclear extracts of cancer cells but absent in those of normal lymphocytes. Moreover, a bidimensional PAGE SouthWestern blot demonstrated that cytotoxic GT oligomers selectively recognized the more basic eEF1A isoform expressed only in cancer cells. These results suggest the involvement of eEF1A, associated with the nuclear-enriched fraction, in the growth and maintenance of tumour cells, possibly modulated by post-translational processing of the polypeptide chain.  (+info)

MFP1 is a thylakoid-associated, nucleoid-binding protein with a coiled-coil structure. (3/28)

Plastid DNA, like bacterial and mitochondrial DNA, is organized into protein-DNA complexes called nucleoids. Plastid nucleoids are believed to be associated with the inner envelope in developing plastids and the thylakoid membranes in mature chloroplasts, but the mechanism for this re-localization is unknown. Here, we present the further characterization of the coiled-coil DNA-binding protein MFP1 as a protein associated with nucleoids and with the thylakoid membranes in mature chloroplasts. MFP1 is located in plastids in both suspension culture cells and leaves and is attached to the thylakoid membranes with its C-terminal DNA-binding domain oriented towards the stroma. It has a major DNA-binding activity in mature Arabidopsis chloroplasts and binds to all tested chloroplast DNA fragments without detectable sequence specificity. Its expression is tightly correlated with the accumulation of thylakoid membranes. Importantly, it is associated in vivo with nucleoids, suggesting a function for MFP1 at the interface between chloroplast nucleoids and the developing thylakoid membrane system.  (+info)

An interaction between a MYC protein and an EREBP protein is involved in transcriptional regulation of the rice Wx gene. (4/28)

We previously demonstrated that a 31-bp nucleotide sequence located upstream of the rice Wx gene played an important role in its expression. We further showed that this cis-acting regulator interacts with nuclear proteins extracted from developing rice endosperm. We used the 31-bp sequence as bait in a yeast one-hybrid system to isolate several cDNA clones from a rice cDNA expression library. One of these cDNAs encodes a MYC protein, designated OsBP-5, which is 335 amino acids long and contains a putative basic helix-loop-helix-ZIP DNA-binding domain. This domain exhibits 50% amino acid sequence identity with the R/B proteins that regulate the expression of genes involved in anthocyanin biosynthesis in plants. The results of electrophoretic mobility shift assays (EMSAs) and Southwestern gel blots indicate that this protein binds specifically to the CAACGTG motif within the 31-bp sequence. However, by itself, the OsBP-5 protein is unable to trans-activate a lacZ reporter gene controlled by the 31-bp sequence when tested in a yeast expression system. Interestingly, OsBP-5 can trans-activate this reporter gene when another protein, OsEBP-89, a member of the EREBP family of transcription factors, is present. Furthermore, in vitro pull-down experiments show that a protein isolated from developing rice endosperm interacts with the OsBP-5 protein, and Western blots confirm that the interacting protein is OsEBP-89. The formation of a supershift band in EMSAs also indicates that two proteins interact with each other. Interference of OsBP-5 gene expression by double-stranded RNA reduces the amylose content in mature seed of transgenic rice plants but has no visible effect on their phenotype. These results suggest that the OsBP-5 and OsEBP-89 proteins act synergistically, perhaps as a heterodimer, to regulate the transcription of the rice Wx gene.  (+info)

Identification of GAPDH as a protein target of the saframycin antiproliferative agents. (5/28)

Saframycin A (SafA) is a member of a class of natural products with potent antiproliferative effects in leukemia- and tumor-derived cells. This activity is frequently conjectured to derive from the ability of saframycins to covalently modify duplex DNA. We used a DNA-linked affinity purification technique to identify GAPDH as a protein target of DNA-small molecule adducts of several members of the saframycin class. Nuclear translocation of GAPDH occurs upon treatment of cancer cells with saframycins, and depletion of cellular GAPDH levels by small interfering RNA transfection confers drug resistance. Roeder and coworkers have recently suggested that GAPDH is a key transcriptional coactivator necessary for entry into S phase. Our data suggest that GAPDH is also capable of forming a ternary complex with saframycin-related compounds and DNA that induces a toxic response in cells. These studies implicate a previously unknown molecular mechanism of antiproliferative activity and, given that one member of the saframycin class has shown efficacy in cancer treatment, suggest that GAPDH may be a potential target for chemotherapeutic intervention.  (+info)

Borrelia turcica sp. nov., isolated from the hard tick Hyalomma aegyptium in Turkey. (6/28)

Previously, a novel, fast-growing spirochaete was isolated from the hard tick Hyalomma aegyptium, which infests tortoises (Testudo graeca), by using Barbour-Stoenner-Kelly (BSK) II medium; the tick samples were taken from the Istanbul area in northwestern Turkey [Guner et al. (2003). Microbiology 149, 2539-2544]. Here is presented a detailed characterization of the spirochaete. Electron microscopy revealed that strain IST7T is morphologically similar to other spirochaetes of the genus Borrelia and possesses 15 to 16 flagellae that emerge from both polar regions. PFGE analysis revealed the genome to comprise a linear chromosome of approximately 1 Mb; two large linear plasmids of approximately 145 and 140 kb, and several small plasmids ranging from 50 to 20 kb in size were also found. The 16S rRNA gene sequence of this Borrelia isolate exhibited 99.4 to 99.8 % identity with other strains isolated from H. aegyptium and less than 99 % similarity with those of other Borrelia species. A phylogenetic tree, generated from 16S rRNA gene sequences, demonstrated that the spirochaete isolates from H. aegyptium clustered together and branched off from both Lyme-disease-related and relapsing-fever-associated Borrelia species. A single copy of the rrs gene was detected in the genome of strain IST7T by Southern hybridization. DNA-DNA hybridization results showed that strain IST7T was distinct from Lyme-disease-related Borrelia, Borrelia burgdorferi and the relapsing-fever-associated species Borrelia hermsii. The G+C content of strain IST7T is 30.0 mol%. From these genetic features, a novel Borrelia species, Borrelia turcica sp. nov., is proposed; the type strain is IST7T (= JCM 11958T = DSM 16138T).  (+info)

Interaction of an approximately 40 kDa protein from regenerating rat liver with the -148 to -124 region of c-jun complexed with RLjunRP coincides with enhanced c-jun expression in proliferating rat liver. (7/28)

The c-jun belongs to the family of proto-oncogenes and encodes for the protein Jun, a component of transcription factor AP-1 involved in regulation of the expression of genes indispensable for cell proliferation and differentiation. While the role of c-jun in the regulation of such genes has been well examined, the regulation of c-jun in proliferating cells is not fully understood. We have earlier reported that the -148 to -124 region of c-jun is involved in the positive regulation of c-jun transcription, and interacts with a positive regulatory factor (rat liver jun regulatory protein; RLjunRP) present in rat liver. In this investigation, we report that this region is differentially recognized in proliferating liver as evidenced by the formation of a complex, different from that observed with normal liver extract. The new complex appears as early as 2 h after partial hepatectomy and its appearance coincides with the rise in c-jun mRNA levels after partial hepatectomy. In regenerating rat liver nuclear extract, an additional protein of approximately 40 kDa (rRLjunRP) interacts with a pre-existing dimer of RLjunRP complexed with the -148 to -124 region of c-jun to form a slow-migrating complex. rRLjunRP appears to pre-exist in the cytosol and translocate to the nucleus as indicated by the continued presence of the retarded complex in nuclear extract prepared from partially hepatectomized rats treated with cycloheximide. UV crosslinking studies, South-Western blot analysis, SDS/PAGE of affinity-purified factor(s), and 2D-PAGE analysis clearly demonstrate that the additional factor induced in response to growth stimulus is an approximately 40 kDa, that binds with the dimer of RLjunRP and enhances the c-jun transcription.  (+info)

A role for UV-light-induced c-Fos: Stimulation of nucleotide excision repair and protection against sustained JNK activation and apoptosis. (8/28)

UV light (UV-C) is a potent inducer of the c-fos gene. Cells lacking c-Fos are hypersensitive to the cytotoxic effect of UV-C indicating a protective role of c-fos induction. Here we show that cells deficient in c-Fos (fos-/-) are unable to remove cyclobutane pyrimidine dimers (CPDs) from DNA and undergo apoptosis at high frequency via the Fas pathway. We also show that in c-Fos-deficient cells the activation of c-Jun N-terminal kinase (JNK) by UV-C differs from the wild-type (wt, fos+/+). In wt cells JNK activation is transient, returning to control level 5-8 h after treatment, whereas in c-Fos-deficient cells JNK activation was long-lasting and did not return to control level. Inhibition of early JNK activation by the JNK inhibitor SP600125 attenuated CPD repair and increased UV-C induced apoptosis whereas inhibition of late JNK activation attenuated the apoptotic response of c-Fos-deficient cells. Late and sustained activation of JNK resulted in upregulation of fas (CD95, apo-1) ligand and induction of caspase 8-dependent apoptosis. The data indicate that the immediate-early induction of the JNK/c-fos pathway stimulates the repair of UV-C induced DNA lesions that protects against apoptosis. If this does not occur, cells do not recover from transcription blockage leading, as shown for c-Fos-deficient cells, to a reduced expression of MKP1, sustained JNK activation and fasL overexpression that finally results in activation of the death receptor pathway. The data support the hypothesis that non-repaired DNA damage is the cause for the late and sustained activation of the MAP kinase pathway in response to genotoxins.  (+info)

An encyclopedia is a comprehensive reference work containing articles on various topics, usually arranged in alphabetical order. In the context of medicine, a medical encyclopedia is a collection of articles that provide information about a wide range of medical topics, including diseases and conditions, treatments, tests, procedures, and anatomy and physiology. Medical encyclopedias may be published in print or electronic formats and are often used as a starting point for researching medical topics. They can provide reliable and accurate information on medical subjects, making them useful resources for healthcare professionals, students, and patients alike. Some well-known examples of medical encyclopedias include the Merck Manual and the Stedman's Medical Dictionary.

I'm not aware of a medical term called "blotting, Southwestern." The term "blotting" in the context of laboratory science refers to a technique used to transfer or visualize molecules from one surface to another, typically using a liquid or gel. "Southwestern" is a geographical term that can refer to a region in the southwestern United States. It's possible that you may be referring to a specific medical or scientific technique that combines blotting and Southwestern, but I was unable to find any relevant information on this topic.

If you meant something different or need more information about laboratory techniques for transferring or visualizing molecules, please let me know!

An oligonucleotide probe is a short, single-stranded DNA or RNA molecule that contains a specific sequence of nucleotides designed to hybridize with a complementary sequence in a target nucleic acid (DNA or RNA). These probes are typically 15-50 nucleotides long and are used in various molecular biology techniques, such as polymerase chain reaction (PCR), DNA sequencing, microarray analysis, and blotting methods.

Oligonucleotide probes can be labeled with various reporter molecules, like fluorescent dyes or radioactive isotopes, to enable the detection of hybridized targets. The high specificity of oligonucleotide probes allows for the precise identification and quantification of target nucleic acids in complex biological samples, making them valuable tools in diagnostic, research, and forensic applications.

DNA-binding proteins are a type of protein that have the ability to bind to DNA (deoxyribonucleic acid), the genetic material of organisms. These proteins play crucial roles in various biological processes, such as regulation of gene expression, DNA replication, repair and recombination.

The binding of DNA-binding proteins to specific DNA sequences is mediated by non-covalent interactions, including electrostatic, hydrogen bonding, and van der Waals forces. The specificity of binding is determined by the recognition of particular nucleotide sequences or structural features of the DNA molecule.

DNA-binding proteins can be classified into several categories based on their structure and function, such as transcription factors, histones, and restriction enzymes. Transcription factors are a major class of DNA-binding proteins that regulate gene expression by binding to specific DNA sequences in the promoter region of genes and recruiting other proteins to modulate transcription. Histones are DNA-binding proteins that package DNA into nucleosomes, the basic unit of chromatin structure. Restriction enzymes are DNA-binding proteins that recognize and cleave specific DNA sequences, and are widely used in molecular biology research and biotechnology applications.

Molecular sequence data refers to the specific arrangement of molecules, most commonly nucleotides in DNA or RNA, or amino acids in proteins, that make up a biological macromolecule. This data is generated through laboratory techniques such as sequencing, and provides information about the exact order of the constituent molecules. This data is crucial in various fields of biology, including genetics, evolution, and molecular biology, allowing for comparisons between different organisms, identification of genetic variations, and studies of gene function and regulation.

A base sequence in the context of molecular biology refers to the specific order of nucleotides in a DNA or RNA molecule. In DNA, these nucleotides are adenine (A), guanine (G), cytosine (C), and thymine (T). In RNA, uracil (U) takes the place of thymine. The base sequence contains genetic information that is transcribed into RNA and ultimately translated into proteins. It is the exact order of these bases that determines the genetic code and thus the function of the DNA or RNA molecule.

Collodion is a clear, colorless, viscous solution that is used in medicine and photography. Medically, collodion is often used as a temporary protective dressing for wounds, burns, or skin abrasions. When applied to the skin, it dries to form a flexible, waterproof film that helps to prevent infection and promote healing. Collodion is typically made from a mixture of nitrocellulose, alcohol, and ether.

In photography, collodion was historically used as a medium for wet plate photography, which was popular in the mid-19th century. The photographer would coat a glass plate with a thin layer of collodion, then sensitize it with silver salts before exposing and developing the image while the collodion was still wet. This process required the photographer to carry a portable darkroom and develop the plates immediately after exposure. Despite its challenges, the wet plate collodion process was able to produce highly detailed images, making it a popular technique for portrait photography during its time.

Thereafter southwestern blotting begins to vary with regards to procedure as since the first blotting's, many more have been ... Southwestern+Blot at the U.S. National Library of Medicine Medical Subject Headings (MeSH) Bowen, B; Steinberg, J; Laemmli, U K ... "Southwestern blot mapping" is a time-efficient way of identifying DNA-binding proteins and specific sites on the genomic DNA ... The southwestern blot, is a lab technique that involves identifying as well as characterizing DNA-binding proteins by their ...
The method is similar to southwestern blot. (2006) Eastern blotting has been used to refer to the detection of fusion proteins ... Western blot Northwestern blot Far-eastern blot Blot Freeze, HH (1993). "Preparation and analysis of glycoconjugates". Current ... The method is similar to southwestern blot. (2011) A recent study used the term eastern blotting to describe detection of ... Since this is essentially a western blot, the charge reversal was used to dub this method an eastern blot. (2005) Eastern blot ...
Southern blot Western blot Northern blot Southwestern blot Eastern blot Gel electrophoresis SDS-PAGE Chromatography ... These techniques include the western blot (protein detection), the northern blot (RNA detection), the southwestern blot (DNA- ... C, Franke; Grafe D; Bartsch H; Bachmann M (2009). Use of non-radioactive detection method for north- and southwestern blot. ... The northwestern blot allows for detection of identified proteins in a way that is inexpensive. The blot is typically a first ...
Southern blot Northern blot Western blot Southwestern blot "Test Blots, Slot Blots & Dot Blots - Immunodetection , Bio-Rad". ... Performing a dot blot is similar in idea to performing a western blot, with the advantage of faster speed and lower cost. Dot ... A dot blot (or slot blot) is a technique in molecular biology used to detect proteins. It represents a simplification of the ... A general dot blot protocol involves spotting 1-2 microliters of a samples onto a nitrocellulose or PVDF membrane and letting ...
Other blotting methods (i.e., western blot, northern blot, eastern blot, southwestern blot) that employ similar principles, but ... acids Restriction fragment Genetic fingerprint Northern blot Western blot Eastern blot Southwestern blot Northwestern blot " ... The names for other blotting methods may follow this convention, by analogy. Southern invented Southern blot after combining ... Southern blot is a time consuming method and can only estimate the size of the DNA since it is a semi-quantitative method. It ...
... making the Far-Western blot a specific case of the Western blot. A southwestern blot is based on Southern blot and is used to ... Southern blot for DNA northern blot for RNA reverse northern blot for RNA western blot for proteins far-western blot for ... A dot blot is a special case of any of the above blots where the analyte is added directly to the blotting matrix (and appears ... The reverse northern blot differs from both northern and Southern blot in that DNA is first immobilized on a blotting matrix ...
Rayner, Julian (1992). Southwestern blotting to establish the composition of UGPB, a putative transcription factor that binds ...
... dynamics Mass spectrometry Isotopic labeling Coimmunoprecipitation Electrophoretic mobility shift assay Southwestern blotting ... reaction Northern blotting Southern blotting Fusion proteins DNA microarray Bioinformatics Flow cytometry Western blotting ... Southern blot - fractionation - Gram stain - Surface Plasmon Resonance - Microscale Thermophoresis DNA sequencing Polymerase ...
... slot blot - SNP - Slc22a21 - SMCR2 - snRNA - snRNP - solution hybridization - somatic cells - Southern blot - southwestern blot ... blotting - blunt end - bone marrow transplantation - box - BP - BRCA1 - BRCA2 - Brix (database) - BSD domain - BURP domain - C ... Western blot - WHEP-TRS protein domain - WIF domain - wildtype - wobble position - Wolfram syndrome - WWE protein domain - XPC- ... dot blot - double helix - downstream (DNA) - downstream (transduction) - drimenol cyclase- ds - duplex - E. coli - Ecotin - EIF ...
Ash from the eruption made landfall on the main island of Tongatapu, blotting out the sun. Loud explosions were heard 65 km (40 ... Tremors and shaking buildings were reported by residents in south-western Niue, around Alofi and Avatele. The United States ...
... king of Sweden Blot-Sweyn, leader of the Swedish pagan renaissance in the 11th century Coifi, priest of the temple at ... in south-eastern Norway and south-western Sweden He is portrayed in Snorri Sturluson's saga Heimskringla. Gibuld (fl. c. 470), ... semi-historical successor to Blot-Sweyn, and the last pagan king in Scandinavia Erik Ringsson, Swedish king and the son of Ring ...
It was discovered by deep sequencing and its transcription was confirmed by Northern blot and RACE analysis. AsdA is estimated ... It is endemic in southwestern United States. The similar S. e. subsp. diarizonae also infects snakes and occasionally humans. ...
... white watermelon rind with the wavering black moon and a few rags of black clouds fast blotting out the white light": All day ... southwestern California including the whole Channel Islands, northwestern and northern Mexico, Yucatan Peninsula, British ...
"Holtzman Ink Blot Test". The Free Dictionary. Archived from the original on 3 August 2020. Retrieved 18 April 2021. " ... "History". Southwestern Psychological Association. Archived from the original on 8 February 2020. Retrieved 27 March 2021. "Ten ... In 1959 he was elected president of the Southwestern Psychological Association and was promoted to professor by the University ...
Symptoms include fading light-green blots on the adaxial side and floury, white, furry blots on the abaxial side. Other hickory ... Rarely, it can be found in southwestern Indiana, and it is uncommon in Maryland. Population decline has been reported in ...
Instead, the attacking player may pin a blot by moving a piece onto the same point. The blot is not removed from the board, but ... from its invention or earliest appearance in Southwestern Asia (or Persia) before AD 800 Tables period: tables games from their ... The blot is 'hit' or 'knocked off' the board and is usually placed on the bar between the two halves of the board. Pinning ... Zeno, who was white, had a stack of seven men, three stacks of two men and two blots, men that stood alone on a point and were ...
... but it also ranges into Wales and north to far southwestern Scotland. It was formerly listed for Ireland, which was based on ... and the three-part posterior spinnerets do not have a light blot. Like other mygalomorph spiders, it has fangs that point ...
The Buocher Höhe is usually seen as the area on the southwestern edge of the forested upland of Berglen, which is sometimes ... Below this thin layer lies Rhätsandstein, which was formerly milled into blotting sand for the drying of ink. The old quarry ...
The PCR and dot blot hybridization (DBH) test was used in 2006-2007. 742 ducks from 70 duck farms were tested. The overall ... "Complete Genome Sequence of the Novel Duck Circovirus Strain GH01 from Southwestern China". Genome Announcements. 1 (1): e00166 ... Scientists have studied Duck circovirus by using polymerase chain reaction (PCR)-based methods and dot blot hybridisation (DBH ...
... blotting, northern MeSH E05.393.525.150 - blotting, southern MeSH E05.393.525.225 - blotting, southwestern MeSH E05.393.525.300 ... blotting, northern MeSH E05.196.181.400.454.655.150 - blotting, southern MeSH E05.196.181.400.454.720 - peptide mapping MeSH ... blotting, northern MeSH E05.196.401.319.670.150 - blotting, southern MeSH E05.196.401.319.720 - peptide mapping MeSH E05.196. ... blotting, western MeSH E05.478.567.320.200.200 - blotting, far-western MeSH E05.478.567.350 - immunoenzyme techniques MeSH ...
Blot, Paris, Coll. De la Chapelle, Paris, Gal, Bénézit, Paris Pablo Picasso, Femme assise dans un fauteil, 1941 Provenance: Gal ... southwestern France. The Einsatzstab Reichsleiter Rosenberg entered the vault in March 1941 to confiscate the art pieces, and ...
He saw Emperor Xiaowu's flight as a blot on his personal history, so he treated Emperor Xiaojing with great formal respect, ... In counterattacks, Western Wei took modern western Henan and southwestern Shanxi. In winter 537, after hearing news that the ...
It was his first attempt at a western and his first novel set in the Southwestern United States, a change from the Appalachian ... He believes there is no reason to "blot the page up with weird little marks". The New York Times described McCarthy's prose in ... Southwestern Writers Collection Series. Foreword by Edwin T. Arnold (Revised and Expanded ed.). University of Texas Press. ISBN ...
In May 2001, CWD was also found in free-ranging deer in the southwestern corner of Nebraska (adjacent to Colorado and Wyoming) ... The brainstem tissue, but not lymph nodes, was positive for CWD (confirmed with Western Blot). A second case of CWD was ... The area in 2006 has expanded to six states, including parts of eastern Utah, southwestern South Dakota, and northwestern ... western blotting (WB), enzyme immunoassay (EIA), protein misfolding cyclic amplification (PMCA), and real time quaking-induced ...
Subsequently, 63% of the population in southwestern Queensland died, especially young animals that were excluded from prime ... And he has been almost blotted out already from some areas.: 127 Despite the growing movement to protect native species, the ...
It was indicated through Western blot that cyclin D1 was upregulated from day 1 to day 60 post-infection. It was also indicated ... T. cruzi transmission has been documented in the Southwestern U.S., and warming trends may allow vector species to move north. ...
The city issued 42 postage stamps in 1945, all showing a heavy black silhouetted outline of the city blotting out Hitler's face ... Schwarzenberg is in the southwestern Ore Mountains. It lies at elevations stretching from 428.5 to 823 metres (1,406 to 2,700 ...
Georgia Southwestern State University Archived 2010-07-06 at the Wayback Machine, Retrieved June 26, 2010. South Georgia ... 73-74https://books.google.com/books?id=svf_7DV9i6UC&pg=PA129&lpg=PA129&dq=remembering+americus+georgia+essays&source=bl&ots= ... now Georgia Southwestern State University), and the South Georgia Trade and Vocational School in 1948 (now South Georgia ... Georgia Southwestern State University, the Windsor Hotel, The Rosalynn Carter Institute for Caregivers, Cafe Campesino, and ...
... blotting out' the information on a milestone which could not be buried easily; the fate of so many other milestones. Another ... then as part of the Glasgow and South Western Railway) on 1 September 1872.and closed permanently to passengers on 6 April 1964 ...
The south western branch traverses to Dow Crag and the south eastern to The Old Man of Coniston, with Goat's Water lying in a ... The view south and west to the coast is excellent, but much of Lakeland is blotted out by the flanks of the Coniston range. The ...
Thereafter southwestern blotting begins to vary with regards to procedure as since the first blottings, many more have been ... Southwestern+Blot at the U.S. National Library of Medicine Medical Subject Headings (MeSH) Bowen, B; Steinberg, J; Laemmli, U K ... "Southwestern blot mapping" is a time-efficient way of identifying DNA-binding proteins and specific sites on the genomic DNA ... The southwestern blot, is a lab technique that involves identifying as well as characterizing DNA-binding proteins by their ...
Hi: I am planning an experiment using the Southwestern blot technique for detection of protein-DNA interaction. Does anyone ... Southwestern Protocol. Sandra Pena spena at upracd.upr.clu.edu Fri Jan 28 10:56:54 EST 2000 *Previous message: Thanx ...
500 was used for South-western blotting in the same study. For immunoblotting of human skeletal muscle tissue extracts, a ... Epidermis protein extracts were generated from human mammary surgery specimens and subjected to western blot using HRP- ... Anti-Mouse IgG (whole molecule)-Peroxidase antibody has been used in western blotting , immunohistochemistry and protein pin ...
Identification of Sequence-Specific DNA-Binding Proteins by Southwestern Blotting Simon Labbe, Jean-Francois Harrisson, and ... Determination of a Transcription Factor-Binding Site by Nuclease Protection Footprinting onto Southwestern Blots Athanasios G. ...
The oil is then successively filtered on blotting paper. The liquid has a yellowish oily profile tending towards brown or green ... Nigella or black cumin is an annual plant of the buttercup family (Ranunculaceae) native to southwestern Asia. That of Egypt ...
Have membrane, will travel: Northwestern, Southwestern, Eastern blots Posted on August 27, 2021. ... When did you last change your foam inserts in the blotting apparatus, the blotting sponge pads? The answer is probably "never ... The quick blot Start with a quick experiment that doesnt require the highest level of sensitivity. In this case, ... I love using fluorescent antibodies instead of the old-fashioned (well, I think they are) HRP-conjugated ones for Western Blots ...
HEXBP binds to single-stranded nucleic acids as demonstrated by gel mobility shift assays and Southwestern blot assays. ...
Images from residents across southwestern Ontario posted to social media show the sun all but blotted out by the intense smoke ... Environment Canada has issued a Special Air Quality Statement for most of southwestern Ontario including the #CityOfTO.. Take ...
Mender, I., LaRanger, R., Luitel, K., Peyton, M., Girard, L., Lai, T. P., Batten, K., Cornelius, C., Dalvi, M. P., Ramirez, M., Du, W., Wu, L. F., Altschuler, S. J., Brekken, R., Martinez, E. D., Minna, J. D., Wright, W. E. & Shay, J. W., Aug 2018, In: Neoplasia (United States). 20, 8, p. 826-837 12 p.. Research output: Contribution to journal › Article › peer-review ...
Southwestern blot. Unlike electrophoretic mobility shift and DNA foot printing, determination of molecular weight of unknown ... "Southwestern blot mapping" is a time-efficient way of identifying DNA-binding proteins and specific sites on the genomic DNA ... In parallel with the EMSA, the proteins on the protein blot also show reduced binding to probes with mutations at the AAATAA ... Furthermore, this element binds specific individual embryonic proteins on a protein blot. The binding activity appears as a ...
In addition, by South-western blotting analysis of nuclear extracts using the 95-bp probe or MRE oligonucleotide probe, we ...
The S-protein was detected by Western blot and its biologic activity was analyzed by binding to Vero cells. Results: Under the ... The S-protein was detected by Western blot and its biologic activity was analyzed by binding to Vero cells. Results: Under the ... The S-protein was detected by Western blot and its biologic activity was analyzed by binding to Vero cells. Results: Under the ... The S-protein was detected by Western blot and its biologic activity was analyzed by binding to Vero cells. Results: Under the ...
Start your free trial to watch Desperate Housewives and other popular TV shows and movies including new releases, classics, Hulu Originals, and more. Its all on Hulu.
Start your free trial to watch Desperate Housewives and other popular TV shows and movies including new releases, classics, Hulu Originals, and more. Its all on Hulu.
For spills, remove excess and blot with a clean cloth dampened with lukewarm water. Repeat with a solution of water and ... ":"Finella Southwestern Vintage Terra Rug","handle":"sydney-finella-southwestern-vintage-terra-distressed-area-rug-2700"," ... ","Size:53 x 73","Size:710 x 910","Size_Group:5 x 7","Size_Group:8 x 11","Southwestern","Style_Southwestern"," ... ":"Finella Southwestern Vintage Terra Rug - 53 x 73","public_title":"53 x 73","options":["53 x 73"],"price": ...
Southwestern, and Moroccan designs. A warm palette of red, blue, and green jewel tones is accented by neutral colors of ivory ... For spills, remove excess and blot with a clean cloth dampened with lukewarm water. Repeat with a solution of water and ... The Tulsa collection draws upon a curated array of traditional Persian, Southwestern, and Moroccan designs. A warm palette of ... ":"Dustin Southwestern Tribal Medallion Crimson Rug","handle":"tulsa-dustin-southwestern-tribal-medallion-crimson-area-rug-tu- ...
Blot spills immediately with a white cloth. Harsh chemicals may damage or fade the rug ... Hand Knotted Black and Ivory Modern Contemporary Southwestern Tribal Trellis Recycled Silk Area Rug , OP11 ... Hand Knotted Black and Ivory Modern Contemporary Southwestern Tribal Trellis Recycled Silk Area Rug , OP11 ... Hand Knotted Black and Ivory Modern Contemporary Southwestern Tribal Trellis Recycled Silk Area Rug , OP11 ...
Blot spills immediately with a white cloth. Harsh chemicals may damage or fade the rug ... Persian Oushak Heriz and Serapi Aubusson Modern Abstract Geometric Moroccan Scandinavian Southwestern ... Home / Collections / Vintage Southwestern Rug / 3x5 Blue Wool Hand Knotted ... .boost-pfs-filter-breadcrumb nav a { color: # ... Home / Collections / Vintage Southwestern Rug / 3x5 Blue Wool Hand Knotted ... .boost-pfs-filter-breadcrumb nav a { color: # ...
1. Remove any loose debris and blot stain.. 2. Pretreat tough stains with water and bleach solution.. 3. Wash on delicate cycle ... Indigo Mudcloth Pillow Cover / Southwestern pillow / 22x22 or 9 other sizes Tribal Pillow / Boho Throw Pillow / Pillow: ...
... and Southwestern (n = 11) regions. Antibodies were determined by Western blot and the muscle tissue was examined by artificial ... Los anticuerpos se determinaron por Western blot y el tejido muscular se examinó por digestión artificial. Se obtuvo una ...
He is active in a legal aid service on the reservation, and he belongs to an organization known as Southwestern Indian ... blotting out the sunlight over a distance of forty miles. ...
Southwestern Rugs. Southwestern rugs are a popular kind of rug that is popular in the United States. They are known for their ... You should immediately blot any spills onto the rug with an a clean, dry cloth. ... They can bring the color and southwestern look to any room with rug that is southwestern. ... Southwestern Rugs can add color and style to any space within your home. There are a variety of sizes available to make it ...
M2012Farming trichothecenes ebook of two , sources of SouthWestern Nigeria. M2005Nigeria Country ,. Good Taxes of ebook بحار ... authoritative oesophageal case of the Nigeria Mycotoxin lightning and function modification, Nigeria fed interferences blot ...
Clean spills immediately; if liquid, blot with clean, undyed cloth by pressing firmly around the spill to absorb as much as ... Home Southwestern Turkish Kilim Bryanna Black/Ivory Wool Rug - 71 x 99 ...
In the case of the blot, there are also some, directional puns in the naming, showing even scientists have a sense of humor. ... The western blotting technique today is a staple of DNA analysis and is performed in labs worldwide. However, its origins are ... Today, the naming convention persists, with scientists referring to the eastern blot, southwestern blot and the far-eastern ... The western blotting technique today is a staple of DNA analysis and is performed in labs worldwide. However, its origins are ...
Blotting, Southern E5.393.525.150 Blotting, Southwestern E5.393.525.225 E5.601.225 E5.601.152 Blotting, Western E5.393.525.262 ... Blotting, Far-Western E5.393.525.262.500 E5.601.262.500 E5.601.495.320.200.500 E5.601.470.320.200.500 Blotting, Northern E5.393 ...
Blotting, Southern E5.393.525.150 Blotting, Southwestern E5.393.525.225 E5.601.225 E5.601.152 Blotting, Western E5.393.525.262 ... Blotting, Far-Western E5.393.525.262.500 E5.601.262.500 E5.601.495.320.200.500 E5.601.470.320.200.500 Blotting, Northern E5.393 ...
Blotting, Southern E5.393.525.150 Blotting, Southwestern E5.393.525.225 E5.601.225 E5.601.152 Blotting, Western E5.393.525.262 ... Blotting, Far-Western E5.393.525.262.500 E5.601.262.500 E5.601.495.320.200.500 E5.601.470.320.200.500 Blotting, Northern E5.393 ...
Blotting, Southern E5.393.525.150 Blotting, Southwestern E5.393.525.225 E5.601.225 E5.601.152 Blotting, Western E5.393.525.262 ... Blotting, Far-Western E5.393.525.262.500 E5.601.262.500 E5.601.495.320.200.500 E5.601.470.320.200.500 Blotting, Northern E5.393 ...
  • Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes. (wakehealth.edu)
  • Southwestern rugs are a popular kind of rug that is popular in the United States. (wasabisys.com)
  • Southwestern Rugs can add color and style to any space within your home. (wasabisys.com)
  • Rugs from the Southwestern region are an excellent option if you're looking to add style and comfort to your home. (wasabisys.com)
  • Southwestern Rugs are a popular style of rug across the United States. (digitaloceanspaces.com)
  • Southwestern rugs are a great way to bring design and color to any space in your home. (digitaloceanspaces.com)
  • It is possible to add an ambiance of color and southwestern design to any space by adding southwest rugs. (digitaloceanspaces.com)
  • Rugs made from the southwestern regions of the United States are typically made out of wool. (digitaloceanspaces.com)
  • Southwestern rugs is a great way to add a splash of color to your space or add some southwest flair. (b-cdn.net)
  • Southwestern rug is among the most sought-after types of rugs found in America. (oraclecloud.com)
  • Southwestern Rugs can be utilized to add a bit of design and color to any space at home. (oraclecloud.com)
  • Southwestern rugs are one of the most sought-after types of rugs found in the United States. (b-cdn.net)
  • Southwestern rugs that will add a bit of style and color to any room in your home. (b-cdn.net)
  • The southwestern blot, is a lab technique that involves identifying as well as characterizing DNA-binding proteins by their ability to bind to specific oligonucleotide probes. (wikipedia.org)
  • Similar to other types of blotting, proteins are separated by SDS-PAGE and are subsequently transferred to nitrocellulose membranes. (wikipedia.org)
  • Southwestern blot mapping" is a time-efficient way of identifying DNA-binding proteins and specific sites on the genomic DNA that they interact with. (wikipedia.org)
  • Identification of Sequence-Specific DNA-Binding Proteins by Southwestern Blotting Simon Labbe, Jean-Francois Harrisson, and Carl Seguin 13. (nhbs.com)
  • He realized that using nitrocellulose paper, instead of chemically modified paper, facilitated the blotting of proteins better. (thedjournal.com)
  • When did you last change your foam inserts in the blotting apparatus, the blotting sponge pads? (advansta.com)
  • Begin by blotting any stains with a dry cotton or white paper towel. (boutiquerugs.com)
  • Care and Cleaning: Blot stains immediately and clean with mild soap and water. (stagandmanor.com)
  • For spills, remove excess and blot with a clean cloth dampened with lukewarm water. (wellwoven.com)
  • Finish by Blotting with water and a clean white cloth. (wellwoven.com)
  • if liquid, blot with clean, undyed cloth by pressing firmly around the spill to absorb as much as possible. (bareens.com)
  • I love using fluorescent antibodies instead of the old-fashioned (well, I think they are) HRP-conjugated ones for Western Blots. (advansta.com)
  • The name originates from a combination of ideas underlying Southern blotting and Western blotting techniques of which they detect DNA and protein respectively. (wikipedia.org)
  • Hi: I am planning an experiment using the Southwestern blot technique for detection of protein-DNA interaction. (bio.net)
  • Epidermis protein extracts were generated from human mammary surgery specimens and subjected to western blot using HRP-conjugated rabbit anti-mouse IgG as the secondary at a 1:80000 dilution in TBSt/5% milk. (anobase.org)
  • Anti-Mouse IgG (whole molecule)-Peroxidase antibody has been used in western blotting , immunohistochemistry and protein pin array assay. (anobase.org)
  • In addition, by South-western blotting analysis of nuclear extracts using the 95-bp probe or MRE oligonucleotide probe, we detected a Zn-dependent DNA-binding protein with a molecular mass of 116 kDa, which is likely to be ZRF. (go.jp)
  • The S-protein was detected by Western blot and its biologic activity was analyzed by binding to Vero cells. (elsevierpure.com)
  • Purified soluble S-protein was readily detected by Western blot with anti-Myc antibody and showed the ability to bind to surface of Vero cells, demonstrating that the soluble S-protein could remain the biologic activity in the native molecule. (elsevierpure.com)
  • With subtle earth tones highlighted by desert-inspired colors in an abstract geometric pattern, the Questa Sunrise Rug - 8 Ft. Round is the perfect addition to your southwestern dining room. (lonestarwesterndecor.com)
  • Western blot and strip immunoblot assays are the most commonly used serologic assays, but others include enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence, and compliment fixation, among others. (medscape.com)
  • The quick blot Start with a quick experiment that doesn't require the highest level of sensitivity. (advansta.com)
  • A southwestern rug is a great way to add an accent of color to your home or to add the southwestern style. (oraclecloud.com)
  • Whether you're looking to add an accent of color or add a touch of southwestern style the southwestern rug is the ideal solution to do it. (b-cdn.net)
  • Images from residents across southwestern Ontario posted to social media show the sun all but blotted out by the intense smoke in the area. (ctvnews.ca)
  • There are several sizes to choose from so that you can find the best Southwestern rug for your area. (digitaloceanspaces.com)
  • Blot the cleaned area dry with a towel. (boutiquerugs.com)
  • As the area under partial eclipse passes over the Pacific and Atlantic Oceans, regions seeing at least the partial eclipse will be much of South America and the southwestern tip of Africa, according to the Observer's Handbook. (skynews.ca)
  • The western blotting technique today is a staple of DNA analysis and is performed in labs worldwide. (thedjournal.com)
  • Western Blot: Western Blot analysis confirmed that POU3F3 knockdown was successful, and provides a platform for further POU3F3 interrogation. (tdl.org)
  • Today, the naming convention persists, with scientists referring to the eastern blot, southwestern blot and the far-eastern blot, for example, as homages to the work done in the 1970s. (thedjournal.com)
  • Once this time has elapsed, the blots are washed to remove any DNA that was not able to bind. (wikipedia.org)
  • 1. Remove any loose debris and blot stain. (annabelbleu.com)
  • split --\u003e\n\u003cp\u003eThe Tulsa collection draws upon a curated array of traditional Persian, Southwestern, and Moroccan designs. (wellwoven.co.uk)
  • The Tulsa collection draws upon a curated array of traditional Persian, Southwestern, and Moroccan designs. (wellwoven.co.uk)
  • They can bring the color and southwestern look to any room with rug that is southwestern. (wasabisys.com)
  • They are available in a range of sizes, which means you'll find the ideal one for Southwestern rug which can be used to add a bit of color and style to any room of your home. (oraclecloud.com)
  • Southwestern rug is a fantastic way to bring the color and elegance to any room of your home. (b-cdn.net)
  • There are a variety of sizes available to make it possible to find the perfect Southwestern rug for your space. (wasabisys.com)
  • A southwestern rug adds style and comfort to your home. (digitaloceanspaces.com)
  • If you're in search of rug that adds both style and comfort to your home, a southwestern rug could be an excellent choice. (b-cdn.net)
  • In addition, quantitative experiments (PCR and Western blot) proved that the expression of MRPL13 was significantly different between LUAD and control samples. (bvsalud.org)
  • Despite that rejection, Burnette's western blotting technique quickly became popular, thanks to the distribution of pre-prints to friends, who replicated the process repeatedly. (thedjournal.com)
  • Burnette had been aware of the Southern blot and sought to honor it, and the location of his work, by naming his technique the western blot. (thedjournal.com)
  • Fifth, the fragments are radioactively labeled and given appropriate time for binding to previously prepared blots. (wikipedia.org)
  • The 53rd Annual Medical Student Research Forum at UT Southwestern Medical Center (Monday, January 26, 2015, 2-5 p.m. (tdl.org)
  • The globally inspired Bedouin collection features an assortment of Southwestern styles that are designed for the contemporary home. (stagandmanor.com)
  • a dilution of 1:500 was used for South-western blotting in the same study. (anobase.org)
  • Harry Towbin and his colleagues at the Friedrich Miescher Institute in Switzerland had created a similar technique that derived inspiration from the northern blot. (thedjournal.com)