Blotting southern. Medical search

Isolation of human transcripts expressed in hamster cells from YACs by cDNA representational difference analysis. (1/10141)

Gene isolation methods used during positional cloning rely on physical contigs consisting of bacterial artificial chromosomes, P1, or cosmid clones. However, in most instances, the initial framework for physical mapping consists of contigs of yeast artificial chromosome (YACs), large vectors that are suboptimal substrates for gene isolation. Here we report a strategy to identify gene sequences contained within a YAC by using cDNA representational difference analysis (RDA) to directly isolate transcripts expressed from the YAC in mammalian cells. The RDA tester cDNAs were generated from a previously reported hamster cell line derived by stable transfer of a 590-kb YAC (911D5) that expressed NPC1, the human gene responsible for Niemann-Pick type C (NP-C). The driver cDNAs were generated from a control hamster cell line that did not contain the YAC that expressed NPC1. Among the gene fragments obtained by RDA, NPC1 was the most abundant product. In addition, two non-NPC1 fragments were isolated that were mapped to and expressed from 911D5. One of these RDA gene fragments (7-R) spans more than one exon and has 98% sequence identity with a human cDNA clone reported previously as an expressed sequence tag (EST), but not mapped to a chromosomal region. The other fragment (2-R) that had no significant sequence similarities with known mammalian genes or ESTs, was further localized to the region of overlap between YACs 911D5 and 844E3. The latter YAC is part of a contig across the NP-C candidate region, but does not contain NPC1. This two-part approach in which stable YAC transfer is followed by cDNA RDA should be a useful adjunct strategy to expedite the cloning of human genes when a YAC contig is available across a candidate interval. (+info)

Human papillomavirus DNA in adenosquamous carcinoma of the lung. (2/10141)

AIM: To investigate the presence of human papillomavirus (HPV) DNA in adenosquamous carcinoma of the lung--which is relatively common in Okinawa but not in mainland Japan--and examine its histological features. METHODS: Of 207 cases where primary lung cancers were surgically removed between January 1995 and June 1997 in Okinawa, 23 were adenosquamous carcinoma. HPV was detected by non-isotopic in situ hybridisation (NISH) and polymerase chain reaction (PCR) amplification with primers specific for E6 and E7 regions of the HPV genome. PCR products were analysed by Southern blotting. Immunohistochemical determination of high molecular weight cytokeratin (HMC) and involucrin was also carried out. RESULTS: 18 cases were positive for HPV DNA by PCR and NISH. HPV types 6, 11, 16, and 18 were found. Seven cases were dual positive for different types of HPV. Using NISH, HPV was also found in the squamous cell components and in neighbouring enlarged adenocarcinoma cells. The HMC and involucrin were demonstrated immunohistochemically in the same areas. CONCLUSIONS: HPV DNA was found in a high proportion (78.3%) of adenosquamous carcinomas in Okinawa, a region where HPV has previously been shown to be prevalent in squamous cell carcinoma of the lung. The adenocarcinoma cells adjacent to the squamous cell carcinoma component were enlarged and positive for HPV, HMC, and involucrin. This is thought to indicate the transition from adenocarcinoma to squamous cell carcinoma. (+info)

The role of alternative splicing of the adhesion molecule, CD44, in lymphoid malignancy. (3/10141)

AIM: To investigate the expression of CD44 isoforms containing variant exon 6 (v6) in a well characterised cohort of patients with non-Hodgkin's lymphoma (NHL) and chronic lymphocytic leukaemia (CLL), and to correlate this with phenotype and disease course. METHODS: Cryostat sections of OCT embedded diagnostic nodal material from NHL patients and cryopreserved mononuclear preparations from CLL patients were used as sources of RNA. After reverse transcription, PCR was carried out with amplimers positioned at either side of the variant exon insertion site to amplify all possible CD44 isoforms. Those isoforms containing v6 were identified after Southern blotting and hybridisation with a radiolabelled oligonucleotide. RESULTS: Of 32 NHL samples analysed, 16 did not express CD44 isoforms containing v6, six expressed an isoform containing exon v6 alone, and 10 expressed v6 long isoforms which contained exon v6 in addition to other variant exons. These data did not correlate with lymphoma classification, disease staging, or the presence or absence of extranodal disease. However, those patients expressing v6 long CD44 isoforms had a worse overall survival than those that did not. The plateau of the survival curves was 50% compared with 82%. No v6 long isoforms were detected in the 21 CLL samples investigated. CONCLUSIONS: The expression of v6 long CD44 isoforms is associated with aggressive disease in NHL, independent of grade, stage, or presence of extranodal disease. (+info)

Structure of cag pathogenicity island in Japanese Helicobacter pylori isolates. (4/10141)

BACKGROUND: cag pathogenicity island (PAI) is reported to be a major virulence factor of Helicobacter pylori. AIM: To characterise cagA and the cag PAI in Japanese H pylori strains. METHODS: H pylori isolates from Japanese patients were evaluated for CagA by immunoblot, for cagA transcription by northern blot, and for cagA and 13 other cag PAI genes by Southern blot. cagA negative strains from Western countries were also studied. Induction of interleukin-8 secretion from gastric epithelial cells was also investigated. RESULTS: All Japanese strains retained cagA. Fifty nine of 63 (94%) strains had all the cag PAI genes. In the remaining four, cag PAI was partially deleted, lacking cagA transcripts and not producing CagA protein. Details of the PAI of these strains were checked; three lacked cagB to cagQ (cagI) and continuously cagS to cag13 (cagII), and the remaining one lacked cagB to cag8. Western cagA negative strains completely lacked cag PAI including cagA. Nucleotide sequence analysis in one strain in which the cag PAI was partially deleted showed that the partial deletion contained 25 kb of cag PAI and the cagA promoter. Interleukin-8 induction was lower with the cag PAI partial deletion strains than with the intact ones. All Japanese cag PAI deleted strains were derived from patients with non-ulcer dyspepsia, whereas 41 of 59 (70%) CagA-producing strains were from patients with peptic ulcers or gastric cancer (p<0.05). CONCLUSIONS: Most Japanese H pylori strains had the intact cag PAI. However, some lacked most of the cag PAI in spite of the presence of cagA. Thus the presence of the cagA gene is not an invariable marker of cag PAI related virulence in Japanese strains. (+info)

Comparison of Bombyx mori and Helicoverpa armigera cytoplasmic actin genes provides clues to the evolution of actin genes in insects. (5/10141)

The cytoplasmic actin genes BmA3 and BmA4 of Bombyx mori were found clustered in a single genomic clone in the same orientation. As a similar clustering of the two cytoplasmic actin genes Ha3a and Ha3b also occurs in another lepidopteran, Helicoverpa armigera, we analyzed the sequence of the pair of genes from each species. Due to the high conservation of cytoplasmic actins, the coding sequence of the four genes was easily aligned, allowing the detection of similarities in noncoding exon and intron sequences as well as in flanking sequences. All four genes exhibited a conserved intron inserted in codon 117, an original position not encountered in other species. It can thus be postulated that all of these genes derived from a common ancestral gene carrying this intron after a single event of insertion. The comparison of the four genes revealed that the genes of B. mori and H. armigera are related in two different ways: the coding sequence and the intron that interrupts it are more similar between paralogous genes within each species than between orthologous genes of the two species. In contrast, the other (noncoding) regions exhibited the greatest similarity between a gene of one species and a gene of the other species, defining two pairs of orthologous genes, BmA3 and HaA3a on one hand and BmA4 and HaA3b on the other. However, in each species, the very high similarities of the coding sequence and of the single intron that interrupts it strongly suggest that gene conversion events have homogenized this part of the sequence. As the divergence of the B. mori genes was higher than that of the H. armigera genes, we postulated that the gene conversion occurred earlier in the B. mori lineage. This leads us to hypothesize that gene conversion could also be responsible for the original transfer of the common intron to the second gene copy before the divergence of the B. mori and H. armigera lineages. (+info)

Molecular cloning and characterization of three cDNAs encoding putative mitogen-activated protein kinase kinases (MAPKKs) in Arabidopsis thaliana. (6/10141)

We isolated three Arabidopsis thaliana cDNA clones (ATMKK3, ATMKK4 and ATMKK5) encoding protein kinases with extensive homology to the mitogen-activated protein kinase kinases (MAPKKs) of various organisms in the catalytic domain. ATMKK3 shows high homology (85% identity) to NPK2, a tobacco MAPKK homologue. ATMKK4 and 5 are closely related to each other (84% identity). Phylogenetic analysis showed that the plant MAPKKs constitute at least three subgroups. The recombinant ATMKK3 and ATMKK4 were expressed as a fusion protein with glutathione S-transferase (GST) in Escherichia coli. Affinity purified GST-ATMKK3 and GST-ATMKK4 proteins contained phosphorylation activity, which shows that both the ATMKK3 and ATMKK4 genes encode functional protein kinases. Northern blot analysis revealed that the ATMKK3 gene expressed in all the organs. The levels of ATMKK4 and 5 mRNAs were relatively higher in steins and leaves than in flowers and roots. We determined the map positions of the ATMKK3, 4 and 5 genes on Arabidopsis chromosomes by RFLP mapping using P1 genomic clones. (+info)

Cloning, expression, and enzymatic characterization of Pseudomonas aeruginosa topoisomerase IV. (7/10141)

The topoisomerase IV subunit A gene, parC homolog, has been cloned and sequenced from Pseudomonas aeruginosa PAO1, with cDNA encoding the N-terminal region of Escherichia coli parC used as a probe. The homolog and its upstream gene were presumed to be parC and parE through sequence homology with the parC and parE genes of other organisms. The deduced amino acid sequence of ParC and ParE showed 33 and 32% identity with that of the P. aeruginosa DNA gyrase subunits, GyrA and GyrB, respectively, and 69 and 75% identity with that of E. coli ParC and ParE, respectively. The putative ParC and ParE proteins were overexpressed and separately purified by use of a fusion system with a maltose-binding protein, and their enzymatic properties were examined. The reconstituted enzyme had ATP-dependent decatenation activity, which is the main catalytic activity of bacterial topoisomerase IV, and relaxing activities but had no supercoiling activity. So, the cloned genes were identified as P. aeruginosa topoisomerase IV genes. The inhibitory effects of quinolones on the activities of topoisomerase IV and DNA gyrase were compared. The 50% inhibitory concentrations of quinolones for the decatenation activity of topoisomerase IV were from five to eight times higher than those for the supercoiling activities of P. aeruginosa DNA gyrase. These results confirmed that topoisomerase IV is less sensitive to fluoroquinolones than is DNA gyrase and may be a secondary target of new quinolones in wild-type P. aeruginosa. (+info)

Reduced pyrazinamidase activity and the natural resistance of Mycobacterium kansasii to the antituberculosis drug pyrazinamide. (8/10141)

Pyrazinamide (PZA), an analog of nicotinamide, is a prodrug that requires conversion to the bactericidal compound pyrazinoic acid (POA) by the bacterial pyrazinamidase (PZase) activity of nicotinamidase to show activity against Mycobacterium tuberculosis. Mutations leading to a loss of PZase activity cause PZA resistance in M. tuberculosis. M. kansasii is naturally resistant to PZA and has reduced PZase activity along with an apparently detectable nicotinamidase activity. The role of the reduction in PZase activity in the natural PZA resistance of M. kansasii is unknown. The MICs of PZA and POA for M. kansasii were determined to be 500 and 125 micrograms/ml, respectively. Using [14C]PZA and [14C]nicotinamide, we found that M. kansasii had about 5-fold-less PZase activity and about 25-fold-less nicotinamidase activity than M. tuberculosis. The M. kansasii pncA gene was cloned on a 1.8-kb BamHI DNA fragment, using M. avium pncA probe. Sequence analysis showed that the M. kansasii pncA gene encoded a protein with homology to its counterparts from M. tuberculosis (69.9%), M. avium (65.6%), and Escherichia coli (28.5%). Transformation of naturally PZA-resistant M. bovis BCG with M. kansasii pncA conferred partial PZA susceptibility. Transformation of M. kansasii with M. avium pncA caused functional expression of PZase and high-level susceptibility to PZA, indicating that the natural PZA resistance in M. kansasii results from a reduced PZase activity. Like M. tuberculosis, M. kansasii accumulated POA in the cells at an acidic pH; however, due to its highly active POA efflux pump, the naturally PZA-resistant species M. smegmatis did not. These findings suggest the existence of a weak POA efflux mechanism in M. kansasii. (+info)

Other blotting methods (i.e., western blot, northern blot, eastern blot, southwestern blot) that employ similar principles, but ... acids Restriction fragment Genetic fingerprint Northern blot Western blot Eastern blot Southwestern blot Northwestern blot " ... A Southern blot is a method used in molecular biology for detection of a specific DNA sequence in DNA samples. Southern ... "Southern Blot". Southern, Edwin Mellor (5 November 1975). "Detection of specific sequences among DNA fragments separated by gel ...

https://en.wikipedia.org/wiki/Southern_blot

Gene expression Northern blot RNA Seq Southern blot Primrose, Sandy B.; Twyman, Richard (2009-04-01). Principles of Genome ... Reverse Northern blot, much like the northern blot upon which it is based, is used to determine levels of gene expression in ... While northern blot or q-PCR are often used to confirm results, both techniques have drawbacks. Northern blot is limited by its ... In comparison to the Northern blot, the reverse northern blot is able to probe a large number of transcripts at once with less ...

https://en.wikipedia.org/wiki/Reverse_northern_blot

A zoo blot or garden blot is a type of Southern blot that demonstrates the similarity between specific, usually protein-coding ... A zoo blot compares animal species while a garden blot compares plant species. The purpose of the zoo blot is to detect the ... Southern blot Fluorescent in situ hybridization Khan, Firdos Alam (20 September 2011). Biotechnology Fundamentals. CRC Press. p ... As a result, zoo blotting is used to detect similar or exact relationships between the DNA in question and other organisms. It ...

https://en.wikipedia.org/wiki/Zoo_blot

Southern blot Western blot Northern blot Southwestern blot Eastern blot Gel electrophoresis SDS-PAGE Chromatography ... Edwin Southern first created the Southern blot, an analytical technique used to detect DNA. The technique involves using gel ... Blot of Protein-RNA Interaction from Young Rice Panicles RNA Isolation and Northern Blot Analysis Protein Blotting Southern, ... These techniques include the western blot (protein detection), the northern blot (RNA detection), the southwestern blot (DNA- ...

https://en.wikipedia.org/wiki/Northwestern_blot

Southern blot Northern blot Western blot Southwestern blot "Test Blots, Slot Blots & Dot Blots - Immunodetection , Bio-Rad". ... Performing a dot blot is similar in idea to performing a western blot, with the advantage of faster speed and lower cost. Dot ... A dot blot (or slot blot) is a technique in molecular biology used to detect proteins. It represents a simplification of the ... A general dot blot protocol involves spotting 1-2 microliters of a samples onto a nitrocellulose or PVDF membrane and letting ...

https://en.wikipedia.org/wiki/Dot_blot

"Southern Blotting , MyBioSource Learning Center". Retrieved 2019-11-11. "Professor Paul Berg , Biographical summary". ... Restriction enzymes were used to linearize DNA for separation by electrophoresis and Southern blotting allowed for the ...

https://en.wikipedia.org/wiki/Molecular_genetics

Named after its inventor, biologist Edwin Southern, the Southern blot is a method for probing for the presence of a specific ... Southern blotting is less commonly used in laboratory science due to the capacity of other techniques, such as PCR, to detect ... A western blot is a technique by which specific proteins can be detected from a mixture of proteins. Western blots can be used ... Brown T (May 2001). "Southern blotting". Current Protocols in Immunology. Chapter 10: Unit 10.6A. doi:10.1002/0471142735. ...

https://en.wikipedia.org/wiki/Molecular_biology

Northern blotting takes its name from its similarity to the first blotting technique, the Southern blot, named for biologist ... Western blot Eastern blot Northwestern blot Far-eastern blot Far-western blot Differential display Gilbert, S. F. (2000) ... The northern blot, or RNA blot, is a technique used in molecular biology research to study gene expression by detection of RNA ... Edwin Southern. The major difference is that RNA, rather than DNA, is analyzed in the northern blot. A general blotting ...

https://en.wikipedia.org/wiki/Northern_blot

Southern blot for DNA northern blot for RNA reverse northern blot for RNA western blot for proteins far-western blot for ... making the Far-Western blot a specific case of the Western blot. A southwestern blot is based on Southern blot and is used to ... The reverse northern blot differs from both northern and Southern blot in that DNA is first immobilized on a blotting matrix ... A dot blot is a special case of any of the above blots where the analyte is added directly to the blotting matrix (and appears ...

https://en.wikipedia.org/wiki/Blot_(biology)

These large regions were termed "ultra-long" telomeres in the literature when they were identified using southern blotting and ... In slot blot (or dot blot), total genomic DNA is attached to a membrane and labeled with a telomere-probe that produces a ... Southern blot). Specialized protocols have demonstrated the ability to isolate high molecular weight Class III telomeric DNA ... Slot blot, however, is conducted without DNA fragmentation or separation, rather whole genomic DNA is used to quantify the ...

https://en.wikipedia.org/wiki/Mega-telomere

This process is termed Southern blotting. For fluorescent dyes, after electrophoresis the gel is illuminated with an ...

https://en.wikipedia.org/wiki/Gel_electrophoresis_of_nucleic_acids

The name originates from a combination of ideas underlying Southern blotting and Western blotting techniques of which they ... Thereafter southwestern blotting begins to vary with regards to procedure as since the first blotting's, many more have been ... "Southwestern blot mapping" is a time-efficient way of identifying DNA-binding proteins and specific sites on the genomic DNA ... Southwestern+Blot at the US National Library of Medicine Medical Subject Headings (MeSH) Bowen, B; Steinberg, J; Laemmli, U K; ...

https://en.wikipedia.org/wiki/Southwestern_blot

A recent highlight article has interviewed Ed Southern, originator of the Southern blot, regarding a rechristening of eastern ... Western blot Northwestern blot Far-eastern blot Blot Freeze, HH (1993). "Preparation and analysis of glycoconjugates". Current ... Since this is essentially a western blot, the charge reversal was used to dub this method an eastern blot. (2005) Eastern blot ... The eastern blot is mentioned in an immunology textbook which compares the common blotting methods (Southern, northern and ...

https://en.wikipedia.org/wiki/Eastern_blot

The name western blot is a play on the Southern blot, a technique for DNA detection named after its inventor, English biologist ... Biology portal Technology portal Chemistry portal Far-eastern blot Far-western blot Eastern blot Northwestern blot Fast ... Edwin Southern. Similarly, detection of RNA is termed as northern blot. The term "western blot" was given by W. Neal Burnette ... However, NC is brittle, and does not allow the blot to be used for re-probing, whereas PVDF membrane allows the blot to be re- ...

https://en.wikipedia.org/wiki/Western_blot

Bulked DNA samples can be analysed using Southern blotting. Use of restriction enzymes or PCR amplification on the DNA is ...

https://en.wikipedia.org/wiki/Bulked_segregant_analysis

Wu W, Zhang HH, Welsh MJ, Kaufman PG (2011). "Characterization of DNA or Genes by Southern Blot Hybridization". Gene ...

https://en.wikipedia.org/wiki/Colony_hybridization

Historically, the method uses Southern blotting to find digested fragments. Tiling arrays have allowed researchers to apply the ...

https://en.wikipedia.org/wiki/Tiling_array

Diagnosing Pearson syndrome utilizes leukocyte DNA with the Southern Blot analysis. This type of mitochondrial DNA deletion are ...

https://en.wikipedia.org/wiki/Pearson_syndrome

This technique is extensively used in Southern blot analysis of DNA samples. In this case, a phosphorus-32-containing DNA probe ...

https://en.wikipedia.org/wiki/Phosphorus-32

Detection of viral DNA is performed by PCR or by Southern blot. Caution is needed in interpreting results from PCR since it is ...

https://en.wikipedia.org/wiki/Merkel_cell_polyomavirus

John Philip Sousa, American composer - sousaphone Edwin Southern, British biologist - Southern blot. Paul-Henri Spaak, Belgian ... Cecil Rhodes, British explorer and industrialist - Northern Rhodesia (Now Zambia), Southern Rhodesia (Now Zimbabwe), Rhodes ...

https://en.wikipedia.org/wiki/List_of_eponyms_(L–Z)

Following the enzyme digestion, a Southern Blot is performed. Southern Blots are a size-based separation method that are ...

https://en.wikipedia.org/wiki/DNA_profiling

One method is the Terminal Restriction Fragment (TRF) southern blot. A Real-Time PCR assay for telomere length involves ...

https://en.wikipedia.org/wiki/Telomere

Southern blotting is still useful in the diagnosis of disorders caused by trinucleotide repeats. Each cell of the body contains ... Southern blotting is an early technique basic on detection of fragments of DNA separated by size through gel electrophoresis ...

https://en.wikipedia.org/wiki/Medical_genetics

Southern blotting A molecular biology method used for detecting a specific sequence in DNA samples. The technique combines ... Contents Top A B C D E F G H I J K L M N O P Q R S T U V W X Y Z See also References External links western blotting whole ... Northern blotting nRNA See nuclear RNA. nuclear membrane A sub-cellular barrier consisting of two lipid bilayer membranes that ...

https://en.wikipedia.org/wiki/Glossary_of_genetics_(M−Z)

It involves dicing the DNA with restriction enzymes and sorting the resulting restriction fragments by size using southern blot ... which is commonly used to visualize the EcoRI fragment during southern blot. The name "p13E-11" reflects that it is a subclone ... which is more precise and less labor-intensive than southern blot.Molecular combing is also available for assessing D4Z4 array ... "Molecular combing compared to Southern blot for measuring D4Z4 contractions in FSHD". Neuromuscular Disorders. 25 (12): 945-51 ...

https://en.wikipedia.org/wiki/Facioscapulohumeral_muscular_dystrophy

An image of this Southern blot can be seen in this article. Bacteriophages are very easily spread. At lower exposures, ... Biochemical tests such as Southern blots can also be used to detect the speA that the phage produces from the speA gene. This ...

https://en.wikipedia.org/wiki/Bacteriophage_T12

"Southern Railway - Budget Highlights-2012-13". Railway budget 2012, Taken from Southern Railways Website. Archived from the ... "Toilets a blot on MRTS", Downtown, The Hindu, Chennai, 26 October 2013. "Chain snatched at MRTS station", The Hindu, Chennai, 1 ... "Southern Railway - Budget Highlights-2012-13". Railway budget 2012, Taken from Southern Railways Website. Archived from the ... "Special trains on MRTS in view of ICC World Cup Cricket Match". Southern Railway Website. Southern Railways. Retrieved 18 ...

https://en.wikipedia.org/wiki/Chennai_Mass_Rapid_Transit_System

He wrote that envoys from Sutrium, Nepete (Romans colonies) and Falerii in southern Etruria arrived in Rome with news that the ... Livy wrote that "the Aequian name was almost blotted out." Still, in 304 BC, the Sabellian peoples of modern northern Abruzzo, ... In 303 BC the Sabine town of Trefula Suffrenas (Ciciliano) and the Volscian town of Arpinium (Arpino) in southern Latium were ... In 307 BC the consul Lucius Volumnius Flamma Violens was assigned a campaign against the Salentini of southern Apulia, where he ...

https://en.wikipedia.org/wiki/Samnite_Wars

The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' ...

https://en.wikipedia.org/wiki/Genetically_modified_brinjal

ELISA screening and Western blot confirming); or 3) single-tier IgG (old infection) Western blot. Previously, the CDC only ... The range of ticks able to carry Lyme disease has expanded from a limited area of Ontario to include areas of southern Quebec, ... Tests for antibodies in the blood by ELISA and Western blot is the most widely used method for Lyme diagnosis. A two-tiered ... Number of cases in southern Europe, such as Italy and Portugal, is much lower. Diagnosed cases in some Western countries, such ...

https://en.wikipedia.org/wiki/Lyme_disease

Georgia Supreme Court Justice David Nahmias, a speaker at the dedication said: "In the final blot that the case placed on the ... "Marker honors Georgia governor who commuted Leo Frank's death sentence". Southern Jewish Life. 25 (7). July 2015. Retrieved ...

https://en.wikipedia.org/wiki/John_M._Slaton

Several Southern states seceded in response to Lincoln's election and formed the Confederate States of America; the American ... Taney delivered "an impassioned defense of Gruber" and, in his opening argument, Taney condemned slavery as "a blot on our ... To avoid the appearance of sectional favoritism, Taney and his Southern colleagues sought to win the support of at least one ... However, after two of the Northern justices objected to the decision, Taney and his four Southern colleagues decided to write a ...

https://en.wikipedia.org/wiki/Roger_B._Taney

Subils., (common name Chiapas red trumpet) endemic to the Montane rainforests of southern Mexico and Guatemala. A small, ... bl&ots=tLt8RWPMU4&sig=ACfU3U0CoKR2hc8ymlfJYJsCoQPTbD0ovg&hl=en&sa=X&ved=2ahUKEwja14DfqIDhAhWgTxUIHROBBmMQ6AEwHnoECAkQAQ#v= ...

https://en.wikipedia.org/wiki/Petunioideae

But it is blotted out in the case of a particular action, insofar as reason is hindered from applying the general principle to ... Southern Economic Association. 39 (4): 490-507. doi:10.2307/1056701. JSTOR 1056701. Rothbard, Murray (Summer 1980). "Ludwig von ... As to those general principles, the natural law, in the abstract, can nowise be blotted out from men's hearts. ... However, while the primary and immediate precepts cannot be "blotted out," the secondary precepts can be. Therefore, for a ...

https://en.wikipedia.org/wiki/Natural_law

In the southern reaches of Redwood Valley is the Ricetti bench, a fluvial terrace the features crimson red soil, where ... terrain with the name Redwood Valley coming from the legends of redwood trees growing so densely packed that they blotted out ...

https://en.wikipedia.org/wiki/Mendocino_County_wine

9] Shira Birnbaum, "Making Southern belles in progressive era Florida: Gender in the formal and hidden curriculum of the ... JET Magazine, Feb 25, 1960 https://books.google.com/books?id=xK8DAAAAMBAJ&pg=PA51&lpg=PA51&dq=breitler+FAMU&source=bl&ots= ... 10] Shira Birnbaum, "Making Southern belles in progressive era Florida: Gender in the formal and hidden curriculum of the ... Marshall, J.Stanley (2006). The Tumultuous Sixties - Campus Unrest and Student Life at a Southern University. Tallahassee: ...

https://en.wikipedia.org/wiki/History_of_Florida_State_University

Rockefeller and a friend briefly left the expedition to study the Asmat tribe of southern Netherlands New Guinea. After ... bl&ots=0nWxxcCC_q&sig=Bt1zD_9PLGiFxKmX447HUrsaN68&hl=en&ei=GdusS4H6DYSBlAfNpojUDQ&sa=X&oi=book_result&ct=result&resnum=3&ved= ...

https://en.wikipedia.org/wiki/Michael_Rockefeller

According to David Lewis-Williams and Jean Clottes who both studied presumably similar art of the San people of Southern Africa ... that was swabbed or blotted on, rather than applied by brush. In other areas, the colour was applied by spraying the pigments ...

https://en.wikipedia.org/wiki/Lascaux

Drown worked in the electrical assembly department of Southern California Edison Company and made her first radionic machine in ... they could call up the clinic who had kept their blood sample on a blotting paper and insert that into the machine, the "blood ... with a sample of their blood she claimed to have in her library saved on blotting paper. When they returned to America she sent ... Metcalf took three samples on blotting paper claiming that they were from her children. Three days later she "received a ...

https://en.wikipedia.org/wiki/Ruth_B._Drown

Mr Blot. Robots by Isaac Asimov: Robbie, Speedy, Cutie, and others, from the stories in I, Robot (1940-1950) (not to be ... Robotech adapted this story from Super Dimension Cavalry Southern Cross Japanese animated series (1984), in which these pilots ...

https://en.wikipedia.org/wiki/List_of_fictional_robots_and_androids

Ridgewell had been in the police force of Southern Rhodesia. Ridgewell's customary behaviour was to dress in plain clothes and ... https://books.google.com/books?id=6KUkEAAAQBAJ&pg=PT26&lpg=PT26&dq=derek+arnold+ridgewell&source=bl&ots=eGt9DYgP4Z&sig= ...

https://en.wikipedia.org/wiki/Derek_Ridgewell

After The Blot, Weber's films did not make money at the box office. For decades, The Blot was considered a lost film, until it ... "A Perpetual Leading Lady", Sunset 32 (Passenger Dept., Southern Pacific Co., 1914): 634. Karen Ward Mahar, Women Filmmakers in ... crafted a melodramatic narrative to bring the issue to life in The Blot. Starring Claire Windsor and Louis Calhern,The Blot was ... The Blot was then produced for video by Kevin Brownlow and David Gill of Photoplay Productions, and released on home video and ...

https://en.wikipedia.org/wiki/Lois_Weber

... "the conduct of the Japanese army as a whole in Nanjing was a blot on the reputation of their country"." Ralph L. Phillips, a ... fire to buildings and houses in the areas close to Xiakuan to the north as well as in the environs of the eastern and southern ...

https://en.wikipedia.org/wiki/Nanjing_Massacre

In the 1990s, the southern thesis was further developed by Charles Bowlus, who wrote that Moravia emerged in the region of the ... have blotted out, but over which in former days [Svatopluk] used to rule. Such are the landmarks and names along the Danube ... Even though the Avar settlement area stabilized on the Danube river in the early period of the khaganate (southern border of ... Péter Püspöki-Nagy proposed the existence of two Moravias: a "Great" Moravia at the southern Morava river in present-day Serbia ...

https://en.wikipedia.org/wiki/Great_Moravia

... separating them through gel electrophoresis and analyzing them via southern blot with probes for the region of interest. This ...

https://en.wikipedia.org/wiki/Epigenomics

... bl&ots=M-h4Rkqlom&sig=vHr43_r-aTciZMEkhx_paZjLK0w&hl=en&sa=X&ei=873XUuGUE86fkgWY44GoAg&ved=0CEUQ6AEwBQ#v=onepage&q=clayton% ... Skye Southern Golf Club, Keysborough Spring Valley Golf Club, Clayton South Woodlands Golf Club, Mordialloc Amstel Park, ...

https://en.wikipedia.org/wiki/Melbourne_Sandbelt

Using Southern blotting and fluorescence in situ hybridization (FISH) on the genome, the researchers found no evidence of DNA ...

https://en.wikipedia.org/wiki/Chimeric_RNA

Cook, Sylvia (1974). "Gastonia: The Literary Reverberations of the Strike". The Southern Literary Journal. 7 (1): 49-66. ISSN ... "completely blotted out" his record as a strike leader and as a victim of the "Gastonia frame up". But the Hearst papers, he ... inflaming the southern jurors and dooming the defendants. Afterward, said he, Communists in Manhattan shipped him and his ...

https://en.wikipedia.org/wiki/Fred_Beal

On the southern front, the army also withdrew but Prome's counterattack captured Razadarit's daughter Tala Mi Kyaw, ... The only blot was that his first wife Tala Mi Daw, who had just delivered their first (and only) son, refused to join him in ... Razadarit did not open the southern front in the 1413-1414 dry season. He decided to save his forces for the likely Ava ... In November, as Hsenwi was running out of provisions, Razadarit reopened the southern front with a massive attack on Prome. It ...

https://en.wikipedia.org/wiki/Razadarit

Feelgood - Disneyland after Dark 1988 (21 August): Venus Juices - Coyote & The Lost Dakota's - Blue Blot - Tröckener Kecks - ... Southern Culture On The Skids - The Jim Jones Revue - Eddie Spaghetti - The Starliters - Reno Divorce - Carlos and the Bandidos ... Southern Culture on the Skids - Heavy Trash - La Muerte - The Sweden Special feat. Domestic Bumblebees and Harmonica Sam - ...

https://en.wikipedia.org/wiki/Sjock_Festival

Gentil knew that the Forreau-Lamy and Voulet missions were marching on southern Chad, respectively from Algeria and Niger. His ... Gentil started navigating the Chari upriver, counting on three cannons, while the steamboat Léon-Blot and their barge had other ...

https://en.wikipedia.org/wiki/Battle_of_Kouno

... bl&ots=1KZXDwp9kv&sig=DfHT_F6ERly8NtZ7MYySWt0v6_o&hl=en&ei=nKO9SueSLoaTkQWE49xW&sa=X&oi=book_result&ct=result&resnum=7#v= ... onepage&q=adiyodi%20caste&f=false Castes and tribes of Southern India, Volume 3 By Edgar Thurston, K. Rangachari Malabar Manual ...

https://en.wikipedia.org/wiki/Thirumulpad

silicicola - Southern juniper Juniperus zanonii (proposed) As of April 2022[update], Plants of the World Online accepts the ... bl&ots=SkBfZip0TX&sig=ACfU3U3FQip4K8a3YUN_xmDfTa45U4d-Ig&hl=en&sa=X&ved=2ahUKEwjt4Zj9zp_7AhV7BzQIHQRwBkgQ6AF6BAgwEAM#v=onepage& ... Adams, R.P., Al-Farsi, A. & Schwarzbach, A.E. (2014). Confirmation of the southern-most population of Juniperus seravschanica ... central and southern Asia, east to eastern Tibet in the Old World, and in the mountains of Central America. The highest-known ...

https://en.wikipedia.org/wiki/Juniper

Though they hunted and persecuted him, they murdered him because they could not conquer him." "He was born in Southern Dakota ... bl&ots=ECBWeOiDgE&sig=rPuoQ_eipSBBwxmznojOxuyCvx8&hl=en&ei=gk99Tb7kKI_6swPv6_yIAw&sa=X&oi=book_result&ct=result&resnum=1&sqi=2& ... bl&ots=COJBQlr1cJ&sig=zVBwjjJqG7_NmkGV22a4RGVy-ow&hl=en&ei=LlB9TeK8CY_urAGn1dHWBQ&sa=X&oi=book_result&ct=result&resnum=1&ved= ...

https://en.wikipedia.org/wiki/Flying_Hawk

"Various - Squares Blot Out The Sun". Discogs.com. Retrieved 3 October 2020. "Various - KFJC 89.7 FM - Fifty Blowing Minds Since ... Jeff Calder (The Swimming Pool Q's) restored the tapes at Southern Tracks Studios in Atlanta and Rodney Mills remastered them ... Squares Blot Out the Sun, Vinyl LP, CD, Cassette, Various artists (1990, DB Recs DB72) Totally Wired, CD, Various artists (1995 ...

https://en.wikipedia.org/wiki/Vanessa_Briscoe_Hay

One of his most important acts in this role was to frustrate an attempt by southern militias and the secret society known as ... General Stone has sustained a most flagrant wrong-a wrong which will probably stand as the very worst blot on the National side ... Southern Secession, Civil War. Baton Rouge: Louisiana State University Press, 2013. ISBN 978-0-8071-5004-7. Catton, Mr. ...

https://en.wikipedia.org/wiki/Charles_Pomeroy_Stone

The other two components of the Southern force were McCulloch's own Western Army, consisting one Louisiana regiment, one Texas ... bl&ots=EdlLfDs0q_&sig=k1xrXV1QU_v1UXa25AvtpbGATLo&hl=en&output=reader Howerton, Bryan R. "First Arkansas Light Artillery ( ... Pearce's Division, comprised one part of the Southern force under the overall command of Confederate Brigadier General Benjamin ... Unable and unwilling to leave the important industrial city undefended, Southern commanders were prevented from moving ...

https://en.wikipedia.org/wiki/1st_Arkansas_Light_Artillery

This unit describes Southern blotting via upward capillary transfer of DNA from an agarose gel onto a nylon or nitrocellulose ... Southern blotting is the transfer of DNA fragments from an electrophoresis gel to a membrane support, resulting in ... This slows down the blotting process and may reduce the amount of DNA that can be transferred. The downward capillary method ... Southern blotting is the transfer of DNA fragments from an electrophoresis gel to a membrane support, resulting in ...

https://www.semanticscholar.org/paper/Southern-Blotting-Brown/d5be241b44768ddfc7f89e9fba1b51850aa420aa

Far-Western blots, Far-Eastern blots, Middle Eastern blots, Southwestern blots etc. that will not be described here. ... Now, of course, the Southern blot has also been adapted for forensic DNA analysis. A number of prisoners have been released ... Southern, Northern, Western, and Eastern blots by Steven A. Edwards, Ph.D. ... Molecular biologists being punny fellows, this was immediately dubbed the Northern blot. Northern blots were invaluable for ...

https://www.aaas.org/southern-northern-western-and-eastern-blots

Dive into the research topics of Southern blot analysis of strain variation in Campylobacter mucosalis. Together they form a ...

https://experts.umn.edu/en/publications/southern-blot-analysis-of-strain-variation-in-campylobacter-mucos/fingerprints/

Schematic diagram of probe used for Southern blotting. (e) Southern blotting analysis using the METE probe (probe 1) on genomic ... Southern blotting. Extracted DNA, 1.5 μg from each sample, was digested with NaeI and BamHI (NEB, Hichin, UK), separated by ... A Southern blotting analysis of genomic DNA prepared from each of the stock-points was carried out, using a probe (338 bp) to ... Western blotting. Total protein was extracted, and western blotting experiments were performed as described by Helliwell et al ...

https://www.nature.com/articles/ismej2014230?error=cookies_not_supported&code=5b9e46f8-adbe-419a-adeb-d09be8c20f7a

Bu H, Chen J, Shen R, Ma L, Xu Y. Study on sensitivity of Southern blotting hybridization using a 32P-labeled probe of PCR ... Bu, H, Chen, J, Shen, R, Ma, L & Xu, Y 1996, Study on sensitivity of Southern blotting hybridization using a 32P-labeled probe ... Bu, H., Chen, J., Shen, R., Ma, L., & Xu, Y. (1996). Study on sensitivity of Southern blotting hybridization using a 32P- ... title = "Study on sensitivity of Southern blotting hybridization using a 32P-labeled probe of PCR products in detecting human ...

https://www.scholars.northwestern.edu/en/publications/study-on-sensitivity-of-southern-blotting-hybridization-using-a-3

Southern blotting. ...

https://www.medscape.com/viewarticle/481641

DNA restriction fragment length plymorphism of the human class II genes as analysed bu Southern blot techniques. / Hui, K.; ... DNA restriction fragment length plymorphism of the human class II genes as analysed bu Southern blot techniques. In ... DNA restriction fragment length plymorphism of the human class II genes as analysed bu Southern blot techniques. ... title = "DNA restriction fragment length plymorphism of the human class II genes as analysed bu Southern blot techniques", ...

https://portalrecerca.uab.cat/en/publications/dna-restriction-fragment-length-plymorphism-of-the-human-class-ii-2

Blotting, Southern * Chromosomes, Human, Pair 2* * Contig Mapping * Gene Library * Humans * In Situ Hybridization ...

https://pubmed.ncbi.nlm.nih.gov/10191101/?dopt=Abstract

Southern blotting was preformed according to the manufacturers instructions of a DIG High Prime DNA Labeling and Detection ... Nucleic Acid Manipulations, Southern Blotting, and Semiquantitative Reverse Transcription-Polymerase Chain Reaction (RT-PCR). ... Southern blotting analysis further confirmed that the target gene ChSAT4 was deleted and replaced by HPH gene in the ΔChsat4 ... Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China ...

https://www.frontiersin.org/articles/10.3389/fmicb.2018.02311/full

Southern Blot. Total cellular DNA was extracted from the transfected HuH7 cells (3 × 106 cells each group) using QIAGEN DNA ... Further, PCR, Southern Blot or direct sequencing confirmed that two HBV-specific gRNAs expressed from gRNA-miR-HBV-gRNA ternary ... Western Blot. Western Blot analysis was performed as previously described [7]. Briefly, the protein lysates (total 60 μg) ... the Cas9/gRNA-mediated destruction of HBV genome was demonstrated by Southern Blot analyses (Supplementary Figures S2A and S2B ...

https://thno.org/v07p3090.htm

Reducing the need to Southern blot for Fragile X; an evaluation of the Asuragen AmplideX FMR1 kit. May 3, 2010. ... HomeAsuragen Molecular Diagnostics PublicationsReducing the need to Southern blot for Fragile X; an evaluation of the Asuragen ...

https://asuragen.com/publications-list/reducing-the-need-to-southern-blot-for-fragile-x-an-evaluation-of-the-asuragen-amplidex-fmr1-kit/

Southern Blot. Southern blot analysis is a laboratory method used to study DNA. Specifically, purified DNA from a biological ... Northern Blot. Northern blot is a laboratory analysis method used to study RNA. Specifically, purified RNA fragments from a ... blot. The method is named for its creator, British molecular biologist Edwin Southern. MORE ... containing complementary sequences with the DNA probe sequence to be visualized within the Southern ...

https://www.genome.gov/genetics-glossary

PCR, long-range PCR, Southern blotting. PCR, Southern blotting. PCR, blotting. Southern blotting, genome mapping. ... mitochondrial or viral DNA from blood and related body fluids ready to use in PCR and blotting procedures. QIAamp sample ...

https://www.qiagen.com/ch/products/discovery-and-translational-research/dna-rna-purification/dna-purification/genomic-dna/qiaamp-dna-blood-kits/

Deletions or rearrangements of the retinoblastoma gene can be detected by either karyotyping or Southern blotting techniques. ... Genetic profile of 81 retinoblastoma patients from a referral hospital in southern India. Indian J Ophthalmol. 2001 Mar. 49(1): ...

https://emedicine.medscape.com/article/1222849-workup

Southern and Western blotting. *UV/Vis spectrophotometry. *Enzyme-linked immunosorbent assays (ELISA) ...

https://www.valpo.edu/biology/experiential-learning/lab-and-field-skills/

A) Representative Southern blots used to identify single copy SAIL and SGT lines. Replicate samples indicate DNA isolated from ... RPP4-RPP5 structural diversity between Arabidopsis accessions analysed by Southern blotting and hybridization. Genomic DNA was ... HRG1 structural diversity between Arabidopsis accessions analysed by Southern blotting and hybridization. Genomic DNA was ... RPP1 structural diversity between Arabidopsis accessions analysed by Southern blotting and hybridization. Genomic DNA was ...

https://www.researchgate.net/profile/Piotr-Ziolkowski-2

Detection of HBV cccDNA by Southern blotting. Genomic DNA and protein-free DNA was isolated from the livers of chimeric mice ... The DNAs were digested with the restriction enzyme, Hind III, that does not cleave HBV, and analyzed by Southern blotting. The ... Finally, we compared ddPCR and Southern blot (SB) analysis using DNA isolated from two chimeric mice in the chronic phase of ... Currently, intrahepatic cccDNA is detected by quantitative real-time PCR (qPCR) or Southern blotting (SB). However, the amount ...

https://www.researchsquare.com/article/rs-583058/v1

... and Western Blots, as well as Restriction Fragment Length Polymorphism. The original Gel-Pro Analyzer was designed to automate ... This software quantitatively analyzes PCR, Northern, Southern, and Western Blots, as well as Restriction Fragment Length ... This software quantitatively analyzes PCR, Northern, Southern, and Western Blots, as well as Restriction Fragment Length ... improved Dot/Slot Blot capabilities, and a built-in saturation warning for over-exposed images. ...

https://www.the-scientist.com/technology/let-a-pro-analyze-your-gels-media-cybernetics-upgrades-gel-pro-analyzer-57349

Southern Blot Method. This is a brief overview of how a Southern blot (more formally called an DNA blot) is performed and what ... Southern blotting (using RFLPs). PCR allows the amplification of a single copy of DNA into millions of copies. However this ... Below is an example of a real Southern blot used to detect the presence of a gene that was transformed into a mixed cell ... In this Southern blot, it is easy to determine which cells incorporated the gene and which ones did not. ...

https://www.chem.fsu.edu/chemlab/chm1020c/Lecture%2012/03.php

Find the right western blotting reagents for the detection and quantitation of proteins and nucleic acids. ... Explore the wide range of western blot detection reagents from PerkinElmer. ... blotting agents for Western or Southern blotting applications.. *Western Lightning™ ONE - Premixed one-component ... Neutral nylon membranes for nucleic acid binding in chemiluminescent and chromogenic Northern and Southern blotting. ...

https://www.perkinelmer.com/category/western-blotting-reagents

B) Southern blot analysis of tail and BM genomic DNA from poly I:C-treated Nfatc1fl/fl (n = 4) and littermate Nfatc1fl/fl, Mx1- ... Southern blotting of genomic DNA and PCR genotyping of Nfatc1 conditional knockout mice. Five micrograms of genomic DNA was ... probe used for Southern blotting and PCR primers (see Methods) employed for genotyping (a, b, and c) are shown. HSV-1 TK, ... E) Western blot analysis for NFATc1 and HSP90 (loading control) in protein lysates from Nfatc1fl/fl (n = 2) and Nfatc1Δ/Δ (n = ...

https://www.jci.org/articles/view/35711

Applications: PCR Amplification , Southern blot analysi , gDNA methylation , Single-nucleotide polymorphism (SNP) Analysis , ... Western blotting: Technique, Theory, and Trouble Shooting Western blotting is an important technique used in cell and molecular ...

https://delos.info/guinea-pig-liver-gdna/

This process is used in common laboratory techniques, such as a Northern or Southern blot. ...

https://www.terrauniversal.com/ovens.php?maximum_temperature=196211&type=199668

uncertainties (exact position not known; Southern blot, PCR, arrayCGH, SNP-array, ...). *Examples - DNA changes ...

https://www.hgvs.org/mutnomen/examplesRNA.html

enzymatic reactions, PCR, Southern blotting. Storage temperature. 15−25 °C. Shelf life (from production). 27 Month(s). ...

https://www.mn-net.com/us/nucleospin-plant-ii-midi-kit-for-dna-from-plants-740771.20

The Southern blot hybridization method additionally allows the identification of potential PHA-producing microorganisms. PCR- ...

https://www.ars.usda.gov/research/publications/publication/?seqNo115=166226

Hematological Significance of Southern Blot Test for bcr-abl Rearrangement and the Breakpoints within Major bcr in Chronic ...

https://koreamed.org/SearchBasic.php?QY=%28%22Journal+of+Clinical+Pathology+and+Quality+Control%22%5BJTI%5D%29+AND+%281997%5BDPY%5D%29+AND+%28Dec%5BDPM%5D%29+AND+%28%222%22%5BISSU%5D%29

Study on sensitivity of Southern blotting hybridization using a 32P-labeled probe of PCR products in detecting human cytomegalovirus. (northwestern.edu)
The Southern blot hybridization method additionally allows the identification of potential PHA-producing microorganisms. (usda.gov)
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for Xp22.32 and Yp11.3, MIC2, and gave a positive result with a probe for Xq28, DXS15. (coriell.org)
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 22q13, D22S45, and 22q12.3q13.1, PDGFB. (coriell.org)
A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES . (bvsalud.org)
This makes the hybridization system ideal for Northern and Southern blots as well as growth of cultures and other applications requiring tightly controlled incubations. (scimetricsinc.com)

We offer a variety of HRP, AP, or Biotin labeled affinity-purified secondary antibodies and proteins offering excellent selectivity for blotting, as well as for ELISA, histochemical, and cytochemical staining procedures. (perkinelmer.com)
This is a comprehensive hands-on, laboratory-based course that introduces students to core techniques such as electrophoretic analysis of nucleic acids and proteins, polymerase chain reaction (PCR), Southern and Western blotting, ELISA, protein purification, tissue culture, DNA cloning, cell culture and Bioinformatics. (georgetown.edu)
All ultra-sound positive cases were further confirmed by dot-blot ELISA. (bvsalud.org)

Three patients underwent genetic analysisof their tumors with polymerase chain reaction amplification of microsatellite regions and southern blot analysis. (oncolink.org)
Clinical Genetic Testing for Fragile X Syndrome by Polymerase Chain Reaction Amplification and Southern Blot Analyses. (cdc.gov)

A trusted portfolio of accurate and efficient horseradish peroxidase (HRP) or alkaline phosphatase (AP) blotting agents for Western or Southern blotting applications. (perkinelmer.com)

Following the same path, new functions of Gel-Pro Analyzer Version 3.0 for Windows 95 and Windows NT include enhanced support for high resolution and high dynamic range images, automatic lane finding with more adjustment parameters, multiple standard lanes and automatic slant detection, improved Dot/Slot Blot capabilities, and a built-in saturation warning for over-exposed images. (the-scientist.com)
Results were independently confirmed using both a Southern blot and slot blot protocol. (cdc.gov)

Many different techniques have been variously described as Eastern blotting, most of which involve using different types of probes to detect post-translational modifications of proteins, for instance the use of lectins to probe a protein-blotted membrane in search of specific carbohydrates. (aaas.org)
Southern-blotting protocol was followed at the genotyping network except for the use of the plasmid pTBN12 as the probe. (cdc.gov)
Using HasNPV partial predicted polyhedrin to probe the Southern blots, we identified the location of the polyhedrin gene within the 6 kb Eco RI, 15 kb Nco I, 20 kb Xho I, 17 kb Bgl II and 3 kb Cla I fragments, respectively. (who.int)

Blotting is one of the most prevalent and useful analytical methods for the detection and quantification of proteins and nucleic acids. (perkinelmer.com)
With PerkinElmer's range of reagents for detecting proteins and nucleic acids in blotting applications, you can have the highest level of sensitivity and specificity for confidence in your results. (perkinelmer.com)
Commonly, nucleic acids or proteins are electrophoretically separated on agarose or acrylamide gels and then transferred (blotted) from the gel onto the NC. (cdc.gov)

The DNA was first treated with restriction enzymes to make the fragments, then subjected to agarose gel electrophoresis to separate them by size, then denatured in situ and transferred to a nitrocellulose membrane by virtue of sucking volumes of buffer through the gel and the membrane, using stacks of paper towels to blot up the buffer. (aaas.org)
Radiolabeled cDNA probes that span various parts of the AR gene, including the entire steroid-binding domain and the DNA-binding domain, as well as part of the 5' region of the gene were used to screen for AR mutations on Southern blots prepared by restriction endonuclease digestion of genomic DNA from human subjects with complete AIS. (elsevier.com)

Protran ™ - Nitrocellulose membranes for high protein binding capacity and retention of small proteins in chromogenic and chemiluminescent Western blotting. (perkinelmer.com)
a western blot analysis is an assay which allows detection of protein present in a extract resolved on polyacrylamide gel by electrophoresis, transfered to a membrane made of nitrocellulose or polyvinylidene difluoride and immobilized using formaldehyde based cross linking. (immport.org)

Western blot is a method in molecular biology/biochemistry/immunogenetics to detect protein in a given sample of tissue homogenate or extract. (immport.org)
Such terms as 'Southern' (transfer of DNA), 'Northern' (transfer of RNA), and 'Western' (transfer of protein) blots have been coined. (cdc.gov)
WESTERN BLOT: "Western blot allows researchers to determine the molecular weight of a protein. (psychologydictionary.org)

The pun was stretched even further to include Western blots, also developed in the Stark laboratory. (aaas.org)
Like Southerns, Western blots are also used for diagnostic assays, for instance, the confirmation of HIV infection. (aaas.org)
There is a whole menagerie of variations on the Southern's original theme: Far-Western blots, Far-Eastern blots, Middle Eastern blots, Southwestern blots etc. that will not be described here. (aaas.org)
Researchers have depended on western blotting for decades to perform proteomic studies and validate many experimental results, including post translational and CRISPR studies. (perkinelmer.com)
With chemiluminescent, fluorescent, and colorimetric detection methods, PerkinElmer's enhanced western blotting substrates, in addition to our nucleic acid detection reagents, can keep your research studies on track and moving forward. (perkinelmer.com)
Western blotting is an important technique used in cell and molecular biology. (delos.info)
The confirmatory HIV test employs a western blot to detect anti-HIV antibody in a human serum sample. (immport.org)
Some forms of Lyme disease testing employ Western blotting. (immport.org)
The Commonwealth War Graves plot is located down the western edge of the southern section of the cemetery, with an entrance in the Rue de Dringhen. (ww1cemeteries.com)

Molecular biologists being punny fellows, this was immediately dubbed the Northern blot. (aaas.org)

Ensure accurate detection with high performance transfer membranes for blotting and other applications. (perkinelmer.com)

Now, of course, the Southern blot has also been adapted for forensic DNA analysis. (aaas.org)
A Southern blot is a method of capturing DNA molecules that have been seperated by agarose gel electrophoresis for subsequent analysis. (immport.org)
d Southern blot analysis of the hph insertion loci. (figshare.com)
All had southern blot or MSP-PCR analysis performed to detect deletion and/or uniparental disomy of the 15q11.2-q13 region. (kcl.ac.uk)

Discussion and Conclusions: The diagnostic technique most frequently used (southern blot), could not discriminate the presence of a deletion or uniparental disomy in two patients, not allowing a genotype-phenotye correlation. (kcl.ac.uk)

Southern blotting is the transfer of DNA fragments from an electrophoresis gel to a membrane support, resulting in immobilization of the DNA fragments, so the membrane carries a semipermanent reproduction of the banding pattern of the gel. (semanticscholar.org)
After Alu I digestion, the DNA was transferred to a nylon membrane for polymorphic guanine cytosine-rich repetitive sequence (PGRS) Southern blotting. (cdc.gov)

However, in other subjects with complete AIS no gross gene mutation was detected on Southern blots. (elsevier.com)
All patients were tested by using PCR and/or southern blot for the FMR1 gene. (who.int)

In 1975, British biologist Edwin Southern published a method for detecting specific DNA fragments after separating them by size. (aaas.org)

To detect the presence of T. cruzi by PCR, to determine the circulating lineages in the species using Southern blot and establish if continuous feedings produced variation in the infection state of the insects, thirty-six specimens corresponding to M. gajardoi were collected in Caleta Vitor, Arica and Parinacota Region. (uchile.cl)

GeneScreen ™ - Neutral nylon membranes for nucleic acid binding in chemiluminescent and chromogenic Northern and Southern blotting. (perkinelmer.com)
Método (desarrollado originalmente por E.M. Southern) para la detección del ADN que ha sido separado electroforéticamente e inmovilizado mediante secado en papel de nitrocelulosa o de otro tipo o en membrana de nylon seguido de la hibridación con SONDAS DE ÁCIDOS NUCLEICOS etiquetadas. (bvsalud.org)

This process is used in common laboratory techniques, such as a Northern or Southern blot. (terrauniversal.com)

Blot with paper & remove spills immediately. (obeetee.in)

As the only direction remaining, the term "Eastern blotting" has been fought over extensively. (aaas.org)

Blotbase , from medicalgenomics.org, is a free database of mRNA sequences quantified by Northern blots from various cell types and tissues. (aaas.org)

for its entry on kigo, Wikipedia lists a selection of Southern Californian seasonal images . (orbific.com)

Southern-cuisine expert and cookbook author Diana Rattray has created more than 5,000 recipes and articles in her 20 years as a food writer. (thespruceeats.com)

The position on the blot and the intensity of the label's signal can be used to estimate RNA size and concentration, respectively. (immport.org)

A total of 3443 people were screened by ultrasound to determine the prevalence of hydatid disease in southern Sudan. (bvsalud.org)

Three out of every four people would live in capital cities located mainly in the southern regions of the continent, and those cities would sprawl out over some of the best farmlands while having totally inadequate roads and railways to serve their rapidly expanding population. (everaldcompton.com)

Use of Dengue Blot IgG Test as an Epidemiological Tool for Assessing Incidence of DHF in Communities. (who.int)

Northern blots were invaluable for detecting and quantifying specific mRNAs, thus determining the activity of different genes. (aaas.org)

Nasopharyngeal carcinoma (NPC) is most common in southern China, and accounts for approximately 20% of all adult cancers Footnote 6 . (canada.ca)

Also on the upside, Newt left the Southern Baptist Church to become something else. (blogspot.com)

Isolation of human transcripts expressed in hamster cells from YACs by cDNA representational difference analysis. (1/10141)

Human papillomavirus DNA in adenosquamous carcinoma of the lung. (2/10141)

The role of alternative splicing of the adhesion molecule, CD44, in lymphoid malignancy. (3/10141)

Structure of cag pathogenicity island in Japanese Helicobacter pylori isolates. (4/10141)

Comparison of Bombyx mori and Helicoverpa armigera cytoplasmic actin genes provides clues to the evolution of actin genes in insects. (5/10141)

Molecular cloning and characterization of three cDNAs encoding putative mitogen-activated protein kinase kinases (MAPKKs) in Arabidopsis thaliana. (6/10141)

Cloning, expression, and enzymatic characterization of Pseudomonas aeruginosa topoisomerase IV. (7/10141)

Reduced pyrazinamidase activity and the natural resistance of Mycobacterium kansasii to the antituberculosis drug pyrazinamide. (8/10141)

Construction of the MsParA (Ms6939) knockout strain of | Open-i

DNA Probes | Profiles RNS

Gene replacement strategy and Southern blot analysis of | Open-i

Southern Blot | Definition of Southern Blot by Merriam-Webster

Difference between revisions of Katherine K. Jacobs: Notebook - OpenWetWare

Re. Southern hyb. - Stringency question

JCI - Altered blood pressure responses and normal cardiac phenotype in ACE2-null mice

TA microsatellite

6860 y[1] w[67c23]; Df(2R)01D09Y-M088/SM6a

Clavam 625 : Side effects, Use and Dosage Related Info

Strengths Available for Clavam | SaveRxCanada.to

Automated design of genomic Southern blot probes - Probe Design 1 - Genes2Cognition Neuroscience Research Programme

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блоттинг-гибридизация | blot hybridization

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