Molecular Probes: A group of atoms or molecules attached to other molecules or cellular structures and used in studying the properties of these molecules and structures. Radioactive DNA or RNA sequences are used in MOLECULAR GENETICS to detect the presence of a complementary sequence by NUCLEIC ACID HYBRIDIZATION.Blotting, Southern: A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.Collodion: A nitrocellulose solution in ether and alcohol. Collodion has a wide range of uses in industry including applications in the manufacture of photographic film, in fibers, in lacquers, and in engraving and lithography. In medicine it is used as a drug solvent and a wound sealant.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Molecular Probe Techniques: The use of devices which use detector molecules to detect, investigate, or analyze other molecules, macromolecules, molecular aggregates, or organisms.Nucleic Acid Hybridization: Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.DNA Probes: Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Molecular Biology: A discipline concerned with studying biological phenomena in terms of the chemical and physical interactions of molecules.Sensitivity and Specificity: Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Reproducibility of Results: The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.Science: The study of natural phenomena by observation, measurement, and experimentation.Gels: Colloids with a solid continuous phase and liquid as the dispersed phase; gels may be unstable when, due to temperature or other cause, the solid phase liquefies; the resulting colloid is called a sol.Digoxigenin: 3 beta,12 beta,14-Trihydroxy-5 beta-card-20(22)-enolide. A cardenolide which is the aglycon of digoxin. Can be obtained by hydrolysis of digoxin or from Digitalis orientalis L. and Digitalis lanata Ehrh.Cetrimonium Compounds: Cetyltrimethylammonium compounds that have cationic detergent, antiseptic, and disinfectant activities. They are used in pharmaceuticals, foods, and cosmetics as preservatives; on skin, mucous membranes, etc., as antiseptics or cleansers, and also as emulsifiers. These compounds are toxic when used orally due to neuromuscular blockade.DNA: A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).Biological Science Disciplines: All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.Audiovisual Aids: Auditory and visual instructional materials.Computer-Assisted Instruction: A self-learning technique, usually online, involving interaction of the student with programmed instructional materials.Autoradiography: The making of a radiograph of an object or tissue by recording on a photographic plate the radiation emitted by radioactive material within the object. (Dorland, 27th ed)Teaching: The educational process of instructing.Software: Sequential operating programs and data which instruct the functioning of a digital computer.Hot Flashes: A sudden, temporary sensation of heat predominantly experienced by some women during MENOPAUSE. (Random House Unabridged Dictionary, 2d ed)Antigen Presentation: The process by which antigen is presented to lymphocytes in a form they can recognize. This is performed by antigen presenting cells (APCs). Some antigens require processing before they can be recognized. Antigen processing consists of ingestion and partial digestion of the antigen by the APC, followed by presentation of fragments on the cell surface. (From Rosen et al., Dictionary of Immunology, 1989)User-Computer Interface: The portion of an interactive computer program that issues messages to and receives commands from a user.Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.Precursor Cell Lymphoblastic Leukemia-Lymphoma: A neoplasm characterized by abnormalities of the lymphoid cell precursors leading to excessive lymphoblasts in the marrow and other organs. It is the most common cancer in children and accounts for the vast majority of all childhood leukemias.Leukemia, Lymphoid: Leukemia associated with HYPERPLASIA of the lymphoid tissues and increased numbers of circulating malignant LYMPHOCYTES and lymphoblasts.Follow-Up Studies: Studies in which individuals or populations are followed to assess the outcome of exposures, procedures, or effects of a characteristic, e.g., occurrence of disease.Precursor B-Cell Lymphoblastic Leukemia-Lymphoma: A leukemia/lymphoma found predominately in children and adolescents and characterized by a high number of lymphoblasts and solid tumor lesions. Frequent sites involve LYMPH NODES, skin, and bones. It most commonly presents as leukemia.Asparaginase: A hydrolase enzyme that converts L-asparagine and water to L-aspartate and NH3. EC 3.5.1.1.Child Day Care Centers: Facilities which provide care for pre-school and school-age children.Philadelphia Chromosome: An aberrant form of human CHROMOSOME 22 characterized by translocation of the distal end of chromosome 9 from 9q34, to the long arm of chromosome 22 at 22q11. It is present in the bone marrow cells of 80 to 90 per cent of patients with chronic myelocytic leukemia (LEUKEMIA, MYELOGENOUS, CHRONIC, BCR-ABL POSITIVE).Bone Marrow: The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.Prognosis: A prediction of the probable outcome of a disease based on a individual's condition and the usual course of the disease as seen in similar situations.Translocation, Genetic: A type of chromosome aberration characterized by CHROMOSOME BREAKAGE and transfer of the broken-off portion to another location, often to a different chromosome.Directories as Topic: Lists of persons or organizations, systematically arranged, usually in alphabetic or classed order, giving address, affiliations, etc., for individuals, and giving address, officers, functions, and similar data for organizations. (ALA Glossary of Library and Information Science, 1983)DirectoryLocation Directories and Signs: Directory signs or listings of designated areas within or without a facility.Laboratories: Facilities equipped to carry out investigative procedures.Review Literature as Topic: Published materials which provide an examination of recent or current literature. Review articles can cover a wide range of subject matter at various levels of completeness and comprehensiveness based on analyses of literature that may include research findings. The review may reflect the state of the art. It also includes reviews as a literary form.Electronic Mail: Messages between computer users via COMPUTER COMMUNICATION NETWORKS. This feature duplicates most of the features of paper mail, such as forwarding, multiple copies, and attachments of images and other file types, but with a speed advantage. The term also refers to an individual message sent in this way.Plasmodium falciparum: A species of protozoa that is the causal agent of falciparum malaria (MALARIA, FALCIPARUM). It is most prevalent in the tropics and subtropics.Actins: Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.Plasmodium: A genus of protozoa that comprise the malaria parasites of mammals. Four species infect humans (although occasional infections with primate malarias may occur). These are PLASMODIUM FALCIPARUM; PLASMODIUM MALARIAE; PLASMODIUM OVALE, and PLASMODIUM VIVAX. Species causing infection in vertebrates other than man include: PLASMODIUM BERGHEI; PLASMODIUM CHABAUDI; P. vinckei, and PLASMODIUM YOELII in rodents; P. brasilianum, PLASMODIUM CYNOMOLGI; and PLASMODIUM KNOWLESI in monkeys; and PLASMODIUM GALLINACEUM in chickens.Plasmodium vivax: A protozoan parasite that causes vivax malaria (MALARIA, VIVAX). This species is found almost everywhere malaria is endemic and is the only one that has a range extending into the temperate regions.Societies, Medical: Societies whose membership is limited to physicians.Plasmodium berghei: A protozoan parasite of rodents transmitted by the mosquito Anopheles dureni.Actin Cytoskeleton: Fibers composed of MICROFILAMENT PROTEINS, which are predominately ACTIN. They are the smallest of the cytoskeletal filaments.Actin Depolymerizing Factors: A family of low MOLECULAR WEIGHT actin-binding proteins found throughout eukaryotes. They remodel the actin CYTOSKELETON by severing ACTIN FILAMENTS and increasing the rate of monomer dissociation.Malaria, Falciparum: Malaria caused by PLASMODIUM FALCIPARUM. This is the severest form of malaria and is associated with the highest levels of parasites in the blood. This disease is characterized by irregularly recurring febrile paroxysms that in extreme cases occur with acute cerebral, renal, or gastrointestinal manifestations.

Isolation of human transcripts expressed in hamster cells from YACs by cDNA representational difference analysis. (1/10141)

Gene isolation methods used during positional cloning rely on physical contigs consisting of bacterial artificial chromosomes, P1, or cosmid clones. However, in most instances, the initial framework for physical mapping consists of contigs of yeast artificial chromosome (YACs), large vectors that are suboptimal substrates for gene isolation. Here we report a strategy to identify gene sequences contained within a YAC by using cDNA representational difference analysis (RDA) to directly isolate transcripts expressed from the YAC in mammalian cells. The RDA tester cDNAs were generated from a previously reported hamster cell line derived by stable transfer of a 590-kb YAC (911D5) that expressed NPC1, the human gene responsible for Niemann-Pick type C (NP-C). The driver cDNAs were generated from a control hamster cell line that did not contain the YAC that expressed NPC1. Among the gene fragments obtained by RDA, NPC1 was the most abundant product. In addition, two non-NPC1 fragments were isolated that were mapped to and expressed from 911D5. One of these RDA gene fragments (7-R) spans more than one exon and has 98% sequence identity with a human cDNA clone reported previously as an expressed sequence tag (EST), but not mapped to a chromosomal region. The other fragment (2-R) that had no significant sequence similarities with known mammalian genes or ESTs, was further localized to the region of overlap between YACs 911D5 and 844E3. The latter YAC is part of a contig across the NP-C candidate region, but does not contain NPC1. This two-part approach in which stable YAC transfer is followed by cDNA RDA should be a useful adjunct strategy to expedite the cloning of human genes when a YAC contig is available across a candidate interval.  (+info)

Human papillomavirus DNA in adenosquamous carcinoma of the lung. (2/10141)

AIM: To investigate the presence of human papillomavirus (HPV) DNA in adenosquamous carcinoma of the lung--which is relatively common in Okinawa but not in mainland Japan--and examine its histological features. METHODS: Of 207 cases where primary lung cancers were surgically removed between January 1995 and June 1997 in Okinawa, 23 were adenosquamous carcinoma. HPV was detected by non-isotopic in situ hybridisation (NISH) and polymerase chain reaction (PCR) amplification with primers specific for E6 and E7 regions of the HPV genome. PCR products were analysed by Southern blotting. Immunohistochemical determination of high molecular weight cytokeratin (HMC) and involucrin was also carried out. RESULTS: 18 cases were positive for HPV DNA by PCR and NISH. HPV types 6, 11, 16, and 18 were found. Seven cases were dual positive for different types of HPV. Using NISH, HPV was also found in the squamous cell components and in neighbouring enlarged adenocarcinoma cells. The HMC and involucrin were demonstrated immunohistochemically in the same areas. CONCLUSIONS: HPV DNA was found in a high proportion (78.3%) of adenosquamous carcinomas in Okinawa, a region where HPV has previously been shown to be prevalent in squamous cell carcinoma of the lung. The adenocarcinoma cells adjacent to the squamous cell carcinoma component were enlarged and positive for HPV, HMC, and involucrin. This is thought to indicate the transition from adenocarcinoma to squamous cell carcinoma.  (+info)

The role of alternative splicing of the adhesion molecule, CD44, in lymphoid malignancy. (3/10141)

AIM: To investigate the expression of CD44 isoforms containing variant exon 6 (v6) in a well characterised cohort of patients with non-Hodgkin's lymphoma (NHL) and chronic lymphocytic leukaemia (CLL), and to correlate this with phenotype and disease course. METHODS: Cryostat sections of OCT embedded diagnostic nodal material from NHL patients and cryopreserved mononuclear preparations from CLL patients were used as sources of RNA. After reverse transcription, PCR was carried out with amplimers positioned at either side of the variant exon insertion site to amplify all possible CD44 isoforms. Those isoforms containing v6 were identified after Southern blotting and hybridisation with a radiolabelled oligonucleotide. RESULTS: Of 32 NHL samples analysed, 16 did not express CD44 isoforms containing v6, six expressed an isoform containing exon v6 alone, and 10 expressed v6 long isoforms which contained exon v6 in addition to other variant exons. These data did not correlate with lymphoma classification, disease staging, or the presence or absence of extranodal disease. However, those patients expressing v6 long CD44 isoforms had a worse overall survival than those that did not. The plateau of the survival curves was 50% compared with 82%. No v6 long isoforms were detected in the 21 CLL samples investigated. CONCLUSIONS: The expression of v6 long CD44 isoforms is associated with aggressive disease in NHL, independent of grade, stage, or presence of extranodal disease.  (+info)

Structure of cag pathogenicity island in Japanese Helicobacter pylori isolates. (4/10141)

BACKGROUND: cag pathogenicity island (PAI) is reported to be a major virulence factor of Helicobacter pylori. AIM: To characterise cagA and the cag PAI in Japanese H pylori strains. METHODS: H pylori isolates from Japanese patients were evaluated for CagA by immunoblot, for cagA transcription by northern blot, and for cagA and 13 other cag PAI genes by Southern blot. cagA negative strains from Western countries were also studied. Induction of interleukin-8 secretion from gastric epithelial cells was also investigated. RESULTS: All Japanese strains retained cagA. Fifty nine of 63 (94%) strains had all the cag PAI genes. In the remaining four, cag PAI was partially deleted, lacking cagA transcripts and not producing CagA protein. Details of the PAI of these strains were checked; three lacked cagB to cagQ (cagI) and continuously cagS to cag13 (cagII), and the remaining one lacked cagB to cag8. Western cagA negative strains completely lacked cag PAI including cagA. Nucleotide sequence analysis in one strain in which the cag PAI was partially deleted showed that the partial deletion contained 25 kb of cag PAI and the cagA promoter. Interleukin-8 induction was lower with the cag PAI partial deletion strains than with the intact ones. All Japanese cag PAI deleted strains were derived from patients with non-ulcer dyspepsia, whereas 41 of 59 (70%) CagA-producing strains were from patients with peptic ulcers or gastric cancer (p<0.05). CONCLUSIONS: Most Japanese H pylori strains had the intact cag PAI. However, some lacked most of the cag PAI in spite of the presence of cagA. Thus the presence of the cagA gene is not an invariable marker of cag PAI related virulence in Japanese strains.  (+info)

Comparison of Bombyx mori and Helicoverpa armigera cytoplasmic actin genes provides clues to the evolution of actin genes in insects. (5/10141)

The cytoplasmic actin genes BmA3 and BmA4 of Bombyx mori were found clustered in a single genomic clone in the same orientation. As a similar clustering of the two cytoplasmic actin genes Ha3a and Ha3b also occurs in another lepidopteran, Helicoverpa armigera, we analyzed the sequence of the pair of genes from each species. Due to the high conservation of cytoplasmic actins, the coding sequence of the four genes was easily aligned, allowing the detection of similarities in noncoding exon and intron sequences as well as in flanking sequences. All four genes exhibited a conserved intron inserted in codon 117, an original position not encountered in other species. It can thus be postulated that all of these genes derived from a common ancestral gene carrying this intron after a single event of insertion. The comparison of the four genes revealed that the genes of B. mori and H. armigera are related in two different ways: the coding sequence and the intron that interrupts it are more similar between paralogous genes within each species than between orthologous genes of the two species. In contrast, the other (noncoding) regions exhibited the greatest similarity between a gene of one species and a gene of the other species, defining two pairs of orthologous genes, BmA3 and HaA3a on one hand and BmA4 and HaA3b on the other. However, in each species, the very high similarities of the coding sequence and of the single intron that interrupts it strongly suggest that gene conversion events have homogenized this part of the sequence. As the divergence of the B. mori genes was higher than that of the H. armigera genes, we postulated that the gene conversion occurred earlier in the B. mori lineage. This leads us to hypothesize that gene conversion could also be responsible for the original transfer of the common intron to the second gene copy before the divergence of the B. mori and H. armigera lineages.  (+info)

Molecular cloning and characterization of three cDNAs encoding putative mitogen-activated protein kinase kinases (MAPKKs) in Arabidopsis thaliana. (6/10141)

We isolated three Arabidopsis thaliana cDNA clones (ATMKK3, ATMKK4 and ATMKK5) encoding protein kinases with extensive homology to the mitogen-activated protein kinase kinases (MAPKKs) of various organisms in the catalytic domain. ATMKK3 shows high homology (85% identity) to NPK2, a tobacco MAPKK homologue. ATMKK4 and 5 are closely related to each other (84% identity). Phylogenetic analysis showed that the plant MAPKKs constitute at least three subgroups. The recombinant ATMKK3 and ATMKK4 were expressed as a fusion protein with glutathione S-transferase (GST) in Escherichia coli. Affinity purified GST-ATMKK3 and GST-ATMKK4 proteins contained phosphorylation activity, which shows that both the ATMKK3 and ATMKK4 genes encode functional protein kinases. Northern blot analysis revealed that the ATMKK3 gene expressed in all the organs. The levels of ATMKK4 and 5 mRNAs were relatively higher in steins and leaves than in flowers and roots. We determined the map positions of the ATMKK3, 4 and 5 genes on Arabidopsis chromosomes by RFLP mapping using P1 genomic clones.  (+info)

Cloning, expression, and enzymatic characterization of Pseudomonas aeruginosa topoisomerase IV. (7/10141)

The topoisomerase IV subunit A gene, parC homolog, has been cloned and sequenced from Pseudomonas aeruginosa PAO1, with cDNA encoding the N-terminal region of Escherichia coli parC used as a probe. The homolog and its upstream gene were presumed to be parC and parE through sequence homology with the parC and parE genes of other organisms. The deduced amino acid sequence of ParC and ParE showed 33 and 32% identity with that of the P. aeruginosa DNA gyrase subunits, GyrA and GyrB, respectively, and 69 and 75% identity with that of E. coli ParC and ParE, respectively. The putative ParC and ParE proteins were overexpressed and separately purified by use of a fusion system with a maltose-binding protein, and their enzymatic properties were examined. The reconstituted enzyme had ATP-dependent decatenation activity, which is the main catalytic activity of bacterial topoisomerase IV, and relaxing activities but had no supercoiling activity. So, the cloned genes were identified as P. aeruginosa topoisomerase IV genes. The inhibitory effects of quinolones on the activities of topoisomerase IV and DNA gyrase were compared. The 50% inhibitory concentrations of quinolones for the decatenation activity of topoisomerase IV were from five to eight times higher than those for the supercoiling activities of P. aeruginosa DNA gyrase. These results confirmed that topoisomerase IV is less sensitive to fluoroquinolones than is DNA gyrase and may be a secondary target of new quinolones in wild-type P. aeruginosa.  (+info)

Reduced pyrazinamidase activity and the natural resistance of Mycobacterium kansasii to the antituberculosis drug pyrazinamide. (8/10141)

Pyrazinamide (PZA), an analog of nicotinamide, is a prodrug that requires conversion to the bactericidal compound pyrazinoic acid (POA) by the bacterial pyrazinamidase (PZase) activity of nicotinamidase to show activity against Mycobacterium tuberculosis. Mutations leading to a loss of PZase activity cause PZA resistance in M. tuberculosis. M. kansasii is naturally resistant to PZA and has reduced PZase activity along with an apparently detectable nicotinamidase activity. The role of the reduction in PZase activity in the natural PZA resistance of M. kansasii is unknown. The MICs of PZA and POA for M. kansasii were determined to be 500 and 125 micrograms/ml, respectively. Using [14C]PZA and [14C]nicotinamide, we found that M. kansasii had about 5-fold-less PZase activity and about 25-fold-less nicotinamidase activity than M. tuberculosis. The M. kansasii pncA gene was cloned on a 1.8-kb BamHI DNA fragment, using M. avium pncA probe. Sequence analysis showed that the M. kansasii pncA gene encoded a protein with homology to its counterparts from M. tuberculosis (69.9%), M. avium (65.6%), and Escherichia coli (28.5%). Transformation of naturally PZA-resistant M. bovis BCG with M. kansasii pncA conferred partial PZA susceptibility. Transformation of M. kansasii with M. avium pncA caused functional expression of PZase and high-level susceptibility to PZA, indicating that the natural PZA resistance in M. kansasii results from a reduced PZase activity. Like M. tuberculosis, M. kansasii accumulated POA in the cells at an acidic pH; however, due to its highly active POA efflux pump, the naturally PZA-resistant species M. smegmatis did not. These findings suggest the existence of a weak POA efflux mechanism in M. kansasii.  (+info)

*Molecular biology

Named after its inventor, biologist Edwin Southern, the Southern blot is a method for probing for the presence of a specific ... Southern blotting is less commonly used in laboratory science due to the capacity of other techniques, such as PCR, to detect ... Brown, T. (1 May 2001). "Southern blotting". Current Protocols in Immunology. Chapter 10: Unit 10.6A. doi:10.1002/0471142735. ... The eastern blotting technique is used to detect post-translational modification of proteins. Proteins blotted on to the PVDF ...

*Mega-telomere

These large regions were termed "ultra-long" telomeres in the literature when they were identified using southern blotting and ... In slot blot (or dot blot), total genomic DNA is attached to a membrane and labeled with a telomere-probe that produces a ... Southern blot). Specialized protocols have demonstrated the ability to isolate high molecular weight Class III telomeric DNA ... Slot blot, however, is conducted without DNA fragmentation or separation, rather whole genomic DNA is used to quantify the ...

*Gel electrophoresis of nucleic acids

This process is termed Southern blotting. For fluorescent dyes, after electrophoresis the gel is illuminated with an ...

*Bulked segregant analysis

Bulked DNA samples can be analysed using Southern blotting. Use of restriction enzymes or PCR amplification on the DNA is ...

*Tiling array

Historically, the method uses Southern blotting to find digested fragments. Tiling arrays have allowed researchers to apply the ...

*Pearson syndrome

Diagnosing Pearson Syndrome utilizes leukocyte DNA with the Southern Blot analysis. This type of mitochondrial DNA deletion are ...

*Merkel cell polyomavirus

Detection of viral DNA is performed by PCR or by Southern blot. Caution is needed in interpreting results from PCR since it is ...

*Reverse northern blot

Gene expression Northern Blot Southern Blot Differential display DNA Microarray qPCR RNA Seq Primrose, Sandy B.; Twyman, ... Reverse Northern blot, much like the northern blot upon which it is based, is used to determine levels of gene expression in ... While northern blot or q-PCR are often used to confirm results, both techniques have drawbacks. Northern blot is limited by its ... In comparison to the Northern blot, the reverse northern blot is able to probe a large number of transcripts at once with less ...

*Bacteriophage T12

An image of this southern blot can be seen in this article. Bacteriophages are very robust biological agents, are very hard to ... Biochemical tests such as southern blots can also be used to detect the speA that the phage produces from the speA gene. This ...

*Medical genetics

Southern blotting is an early technique basic on detection of fragments of DNA separated by size through gel electrophoresis ... Southern blotting is still useful in the diagnosis of disorders caused by trinucleotide repeats. Short tandem repeats are ...

*Mycobacterium ulcerans

Southern blot analysis to detect IS2404 and IS2606 shows inconclusive RFLP patterns between different strains. Due to the high ... limiting the value of the Southern blot method to type M. ulcerans isolates. Jackson et al. have used pTBN12, a well-defined ...

*Zoo blot

A zoo blot or garden blot is a type of Southern blot that demonstrates the similarity between specific, usually protein-coding ... A zoo blot compares animal species while a garden blot compares plant species. The purpose of the zoo blot is to detect the ... Southern blot Fluorescent in situ hybridization Khan, Firdos Alam (20 September 2011). Biotechnology Fundamentals. CRC Press. p ... As a result, zoo blotting is used to detect similar or exact relationships between the DNA in question and other organisms. It ...

*Bt brinjal

The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' ...

*Archaeogenetics

conducted southern blot hybridization on Neanderthal aDNA (extracted from fossil remain W-NW and Krapina). The results showed ... Studying haplogroups has led some scientists to conclude that a southern migration into the Americas from one small population ... Genetic analysis has supported archaeological hypotheses of a large-scale migrations of Bantu speakers into Southern Africa ...

*Breus' mole

Evidence from Southern blot test reveals that 85 percent of the clotted material is maternal blood. Breus mole is reported to ...

*PstI

Its use is not limited to molecular cloning; it is also used in restriction site mapping, genotyping, southern blotting, ...

*Polysomy

Mutations in chromosome 4 are able to be visualized when RFLP is used in conjunction with Southern blot analysis. Human ...

*Restriction enzyme

In a similar manner, restriction enzymes are used to digest genomic DNA for gene analysis by Southern blot. This technique ...

*Chimeric RNA

Using Southern blotting and fluorescence in situ hybridization (FISH) on the genome, the researchers found no evidence of DNA ...

*Transposon mutagenesis

Colonies that underwent random transposition events were identified by BamHI digestion and Southern blotting using an internal ...

*Gene expression profiling in cancer

DNA microarrays evolved from Southern blotting which allows for detection of a specific DNA sequence in a sample of DNA. Due to ...

*DNA extraction

Using the Southern blot technique, this quantified DNA can be isolated and examined further using PCR and RFLP analysis. These ...

*Northwestern blot

Southern blot Western blot Northern blot Southwestern blot Eastern blot Gel electrophoresis SDS-PAGE Chromatography ... Edwin Southern first created the Southern blot, an analytical technique used to detect DNA. The technique involves using gel ... These techniques include the Western blot (protein detection), the Northern blot (RNA detection), the Southwestern blot (DNA- ... With a Southern Blot, the separated DNA fragments are then transferred to a filter membrane for detection. Detection occurs as ...

*Edwin Southern

The northern blot, western blot and eastern blot, related procedures for the analysis of RNA, protein and post-translational ... The Southern blot is used for DNA analysis and was routinely used for genetic fingerprinting and paternity testing prior to the ... Southern has devised valuable methods for DNA analysis. His 'blot' technique, for the identification of specific sequences ... The concepts of the Southern blot were used in the development and creation of the modern microarray slide, which is an ...

*History of polymerase chain reaction

The use of DNA polymerase for nick translation was the most common method used to label DNA probes for Southern blotting. In ... In November 1984 the amplification products were analyzed by Southern blotting, which clearly demonstrating increasing amount ... "A dot-blot screening procedure for mutated ras oncogenes using synthetic oligodeoxynucleotides." Gene vol. 50(1-3) pp. 313-20 ( ...

*David Martin (sociologist)

He also served as Scurlock Professor of Human Values at Southern Methodist University, Dallas, Texas, USA, 1986-1990. He spent ... bl&ots=ylppIwP2f2&sig=HjLVKHNERdkot0YVO3RCFUhkEgw&hl=en&sa=X&ved=0ahUKEwj8vuy0-obKAhVJaxQKHZnvBzQ4HhDoAQgzMAU#v=onepage&q= ...
Construction of the MsParA (Ms6939) knockout strain of M. smegmatis and Southern blot assays.(A) Schematic representation of the recombination strategy for the
Gene replacement strategy and Southern blot analysis of progeny from heterozygous crosses. (a) The wild-type P-selectin allele, the replacement vector, and th
Southern blot definition is - a blot consisting of a nitrocellulose or nylon sheet containing spots of DNA for identification by a suitable molecular probe.
Restriction digest, also known as DNA fragmentation, is a method to cut DNA into smaller fragments of interest with restriction enzymes. In our experiment, we performed double digests which involves cutting the vector and insert DNA with two different restriction enzymes. XbaI and PstI restriction enzymes are used to cut the insert, in this case Green Fluorescent Protein (GFP). SpeI and PstI are used to cut the plasmid (ROO40 and R0010). GFP is designated as the insert because it is much larger (approximately 700 bp) than R0040 or R0010. If R0040 or R0010 were to be used as inserts, they would not show up clearly during the gel electrophoresis. It is important to use different restriction enzymes to ensure correct insert orientation during ligation with the vector. Things to take into account when performing a restriction digest: (1) Buffer compatibility: When performing a single digest, a buffer with a low salt concentration should be used first followed by a buffer with a higher salt ...
Yng Chen writes: , In Southern hybridization experiments how important is hybridization , temperature? What are the effects of rasing or lowering the hybridization , temperature? One generally hybridizes 15-20 C below the Tm to get favorable kinetics. So the exact temp. is not critical with respect to enforcing stringency. The only exception is if youre trying to do something at very low stringency, then you may need to lower the hybridization temp. below standard conditions. , How does one determine the proper washing conditions (assuming 100% , match in base paring). [for oligo probes] , If you get a weak signal and you know that you have a lot of DNA , on the membrane and that you have good specific activity on your , probe, how do you know if this is because your washing conditions , are too strigent or that the signal is non-specific. You start with a calculation, and then confirm by doing a temperature series against a cloned control target. If youre trying to discriminate a one base ...
Generation of ACE2-deficient mice. BAC145d21 containing a portion of the murine Ace2 gene was identified by Southern hybridization using a 1.8-kb EcoRI fragment of the human ACE2 cDNA. A 5.2-kb fragment of BAC145d21 that included the exon containing sequences encoding the active site of the ACE2 enzyme (+1069 to +1299) was subcloned into the yeast shuttle vector YCpLac22 (pMD44). A 3.4-kb fragment containing the NEO/URA3 cassette from pRAY-1 and flanked by Ace2 genomic sequence was generated by PCR and cotransformed with pMD44 into yeast strain YPH501 for homologous recombination. The final linearized targeting construct, shown in Figure 1A, was electroporated into MPI1-12D ES cells that had been derived from 129/SvEvfBRTac mice. G418-resistant ES cells were screened for homologous recombinants by Southern blot hybridization with genomic probes that were outside of the targeting construct (Figure 1, A and B). Targeted ES clones were then injected into C57BL/6H blastocysts to generate chimeras. ...
Dear Netters: I am screening A. thaliana genomic library with a heterologous probe. By screening about 160000 plaques(!), I have got 9 independent clones. These clones are purified to homogeneity by 3-5 time screening. All washes were to high stringency. To double check that these (or at least one of them) are corresponding to the right gene and not other related genes (i.e. other members of a gene family), I do the following test primarily. That is, I made a southern blot of A. thaliana genomic DNA digested by 3 different restriction enzymes. Then, I hybridize the original probe (which was used for screening) and the probe made from the whole insert (9-17 Kb) of the isolated clones. I expected that the banding pattern of the S. blots of the isolated clones to be similar (or include) that of the S. blot probed with the screening probe. This did not happened for any of the isolated clones. Any advice or comment regarding this problem is greatly appreciated. Thanks. Ali My E-Mail Address is: ...
P{y[+t7.7]=3wHy} associated with end of Df; Hobo element may be mobilized if crossed to H strain, B.G. Cytological breakpoints estimated from molecular breakpoints, K.C ...
Important Information about Clavam 625 - an Antibiotic medicine. Why it is prescribed, dosage and possible side-effects of Clavam 625
Hello SIR, Thanks for your reply. Today is 20th day and still my urologist kept me on half dose of clavam 625 and levoflox 250 twise a day for another 5 days.. ...
Southern blotting is an experimental procedure where DNA, from a genomic or other source, is digested with a restriction enzyme and then separated by size using gel electrophoresis. The fragments are transferred from the gel onto a membrane (blotted) which is then incubated with a labelled single-stranded DNA probe. Such a procedure allows one to locate a particular sequence of DNA within a complex mixture of DNA. From a gene targeting perspective, Southern blotting can be used to detect whether a targeting event has successfully taken place. Designing a good Southern blot probe for a particular gene or locus involves finding a stretch of DNA sequence at that locus, generally 500-1000bp long, that has the desirable qualities of being unique to that locus, with little or no repetitive DNA content. Molecular biologists tend to design their probes manually, by excising portions of genome sequence from online genome browsers (such as such as Ensembl) and then pasting them into a genome-search ...
Revision as of 14:33, 2 October 2019 by Webref (talk , contribs) (Created page with A procedure in which DNA restriction fragments are transferred from an agarose gel to a nitrocellulose filter, where the denatured DNA is then hybridized to a radioactive prob...) ...
Genetic information processingMobile and extrachromosomal element functionsPlasmid functionsentry exclusion protein TrbK (TIGR04361; HMM-score: 9.9) ...
DIG southern blot - posted in Molecular Biology: Please can someone give me some suggestions as to why my DIG SB is not working. Only the DIG-labeled ladder and positive control work. I have tried different DNA concentrations, less stringent hyb/washes. I know this protocol should work as it works for people in another country working on the same plant. I used the same probe and followed their protocol. the only difference is the TNA extraction (I use CTAB Doyle and Doyle), and NBT instead...
Southern blot analysis indicated the presence of a single bar gene flanked its regulatory genetic elements. further analysis indicated the the 3 nos terminator was truncated during the transformation process. Additionally a second copy of the 35S promoter or part of it was inserted into the host genome ...
Hi, everyone;. I have done a series of PCR and found that there is slight difference between control and treated group according to bands stained with EB in 2% agarose gel. I was told that such a result is much less sensitive than that of southern. So I blotted such gel to membrane for further southern analysis. Here is the question, if I can use the same PCR product to construct my probe? Yes or No? and How ...
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The aim of this study was to demonstrate that good characterization of S. cerevisiae clinical isolates can be achieved by using a molecular method such as Southern blot hybridization analysis. Even though the development of a molecular typing method useful for the characterization of a yeast infection is a desirable goal for many mycologists, the number of studies in which the epidemiology of S. cerevisiae infections has been investigated is very limited (9, 17, 34). A universally accepted typing method for this organism has been not defined. This could be due in part to the low prevalence of diseases caused byS. cerevisiae, particularly of vaginal infections. However, in our investigation, these infections have been of considerable interest. As described above, of 513 women who visited our Microbiological Service in the study period, 5.8% had S. cerevisiae in their vaginal swabs. This was in agreement with the data reported by Agatensi et al. (1), by which the incidence of vaginal infections ...
The deletion mutation was introduced into the ABI 2.1 embryonic stem (ES) cell line (Soriano et al., 1991) and transmitted to the germline as described previously (Bullard et al., 1996). Chimeric mice were bred with C57BL/6J mice, and the mice used in these studies were maintained on a mixed 129/SvEv and C57BL/6J background. The mutation is being back-crossed on the C57BL/6J background for future work.. Southern, Northern, and Western analyses. Southern blot hybridization was performed according to standard methods (Sambrook et al., 1989) using a hybridization solution of 0.125m NaPO4, pH 7.0, 0.25 mNaCl, 1 mm EDTA, 10% polyethylene glycol (PEG-8000), 7% SDS, and 1% bovine serum albumin (BSA) at 65°C overnight followed by washing to a final stringency of 0.2× SSC/0.1% SDS at 65°C and autoradiography at −80°C.. Total RNA was isolated from brain after homogenization in Ultraspec II (Tel Test, Houston, TX). Total RNA was resolved on a 1.2% agarose gel in 10 mm NaPO4 buffer, pH 6.8, after ...
Genetic information processingMobile and extrachromosomal element functionsProphage functionsputative phage terminase, small subunit, P27 family (TIGR01558; HMM-score: 13.7) ...
OBJECTIVES--To determine the prevalence of urethral HPV infection, as indicated by the presence of HPV DNA in semen, in males with and without penile warts. DESIGN--Prevalence study of HPV types 6/11 and 16 DNA using PCR and Southern blot hybridisation analysis of semen. SETTING--Department of Genitourinary Medicine, Blundell Street Clinic, Leeds General Infirmary and the Assisted Conception Unit (ACU) Kings College, London. SUBJECTS--Patients attending the Genitourinary Clinic for treatment of sexually transmitted diseases including penile warts and males attending Kings ACU for investigations of infertility. MAIN OUTCOME MEASURES--HPV DNA detected by polymerase chain reaction (PCR) and/or Southern blot hybridisation in semen. RESULTS--HPV DNA was detected by PCR in 23 of 27 (85%) specimens from patients attending the GUM clinic for treatment of genital warts and in one of two specimens from patients attending the clinic for other conditions. By Southern blot, nine (33%) of the 29 specimens ...
We present data demonstrating the gene expression of substance P and its receptor in human peripheral blood-isolated monocytes and macrophages. Using the RT-PCR assay, preprotachykinin-A (substance P) mRNA is detected in human peripheral blood-isolated monocytes and macrophages. Among the alpha, beta, and gamma transcripts of the substance P gene, only the beta and gamma transcripts are detectable in these cells. By Southern blot assay these RT-PCR-amplified transcripts are recognized using a specific substance P probe. Sequence analysis of the RT-PCR products from both monocytes and macrophages also confirmed the structure of these transcripts, which are identical to those found in human neuronal cells. At the protein level, both human monocytes and macrophages produced endogenous substance P as determined by an enzyme immunoassay. Capsaicin, a vanillyl fatty acid amide (ingredient of hot pepper), released substance P from both human monocytes and macrophages. In addition, using nested RT-PCR ...
Transgene vector and generation of transgenic mice.We obtained the Crx genomic clone from a 129SVJ mouse library (Stratagene, La Jolla, CA) by using a mouse Crx cDNA probe. We ligated and subcloned a 10 kb XhoI (partial digestion)-EcoRI fragment and a PCR-amplified 2 kbEcoRI-SmaI fragment containing exon 1 into a pβ-gal-Basic vector (Clontech, Palo Alto, CA) to make the Pcrx12k-lacZ construct (see Fig. 1A). Sequencing verified the 2 kb EcoRI-SmaI fragment. The Pcrx2k-lacZ vector contains the 2 kbEcoRI-exon 1 fragment and a 10 kbSmaI-EcoRI fragment that contains the first intron.. We extracted the Pcrx2k-lacZ and the Pcrx12k-lacZ from the recombinant plasmids by aNotI and SalI digestion. We fractionated theNotI-SalI fragments by electrophoresis on a 0.8% agarose gel and purified them by electroelution in dialysis tubes. We microinjected the DNA fragment into pronuclei of B6SJL/F2 C57BL/6 × SJL F2 hybrids. Then Southern blot hybridization of a HindIII-ClaI 954 bp fragment of the β-galactosidase ...
This was a hybrid restriction site created during the cloning process (Cap-trapper) for the library creation. Neither of these restriction sites will work to digest the insert from the vector. According to the IMAGE consortium the SalI-XhoI (gtcgag) is located at position 742 of the polylinker sequence (http: //mgc. nci. nih. gov/Vectors/vec_pbluescriptr). Customers can use BamHI (5) and EcoRI (3) to digest out the insert. Other options for sub-cloning are either to use different restriction enzymes lying outside BamHI and EcoRI or to design insert-specific primers based on the insert sequence. You can also find a reference for the cap trapper method here: Carninci et. al. , DNA RESEARCH 4, 61-66 (1997), High Efficiency Selection of Full-length cDNA by Improved Biotinylated Cap Trapper ...
Nucleic acids from ATCC Genuine Cultures can save you the time and expense of isolating DNA yourself. ATCC offers genomic DNA from well-characterized and authenticated fungal and yeast strains.
Nucleic acids from ATCC Genuine Cultures can save you the time and expense of isolating DNA yourself. ATCC offers genomic DNA from well-characterized and authenticated fungal and yeast strains.
Developmentally programmed deoxyribonucleic acid(DNA) rearrangements are structural reorganisations of the genome that occur reproducibly during the development of a variety of organisms
12404385] The Biosynthesis of Vancomycin-Type Glycopeptide Antibiotics-The Order of the Cyclization Steps This work was supported by the Deutsche Forschungsgemeinschaft (SFB 323) and by a grant of the EU (MEGATOP, QLK3-1999-00650). R. D. S. gratefully acknowledges the support of a Feodor-Lynen Fellowship granted by the Alexander-von-Humboldt Stiftung. We thank Corina Bihlmaier and Volker Pfeifer for help with transformation and Southern hybridization, J. A. Moss (La Jolla (USA)) for critical comments on the manuscript and Prof. Dr. M. E. Maier and Prof. Dr. H.-P. Fiedler (Tubingen) for generous support. (Angew Chem Int Ed Engl. , 2001 ...
Size: 201.55 Kb.; Быстрые, не зависимые от транскрипции эффекты прогестерона, называемые внегеномными, были

North Carolina Biotechnology Center company directory : a directory of bioscience companies in North Carolina :: State...North Carolina Biotechnology Center company directory : a directory of bioscience companies in North Carolina :: State...

Southern blot analysis, Western blot analysis, cDNA cloning and mouse tail genotyping. www.celplor.com CEM Corp. (Matthews) ... Southern blot analysis, Western blot analysis, cDNA cloning and mouse tail genotyping. www.celplor.com CEM Corp. (Matthews) ... Southern Pines, NC 28387. Telephone 910-692-7271. Fax 910-692-9382. Editorial content provided by NCBiotech Library. All rights ... Southern Pines, NC 28387. Telephone 910-692-7271. Fax 910-692-9382. Editorial content provided by NCBiotech Library. All rights ...
more infohttp://digital.ncdcr.gov/cdm/ref/collection/p249901coll22/id/417232/

Glossary:Southern BlotGlossary:Southern Blot

An assay that detects specific DNA molecules using a DNA or RNA probe with sequence similarity. Samples are subjected to electrophoresis on a slab gel. A replica of the gel is then made on a membrane by capillary transfer following denaturation. Specific DNA sequences are then detected on the membrane with a radioactively- or chemically-labeled probe. See the Figure from Alberts, et al., Molecular Biology of the Cell ...
more infohttp://www.informatics.jax.org/glossary/southern_blot

Southern blot | Define Southern blot at Dictionary.comSouthern blot | Define Southern blot at Dictionary.com

Southern blot definition, a procedure for identifying and measuring the amount of a specific DNA sequence or gene in a mixed ... Origin of Southern blot. after Edwin M. Southern, originator of the technique ...
more infohttps://www.dictionary.com/browse/southern-blot

Southern Blotting | Thermo Fisher Scientific - USSouthern Blotting | Thermo Fisher Scientific - US

Southern blot analysis reveals information about DNA identity, size, and abundance. It is a classic technique that involves ... Southern blot analysis reveals information about DNA identity, size, and abundance. It is a classic technique that involves ... Obtaining complete fragmentation of your DNA at the intended restriction enzyme sites is a critical step in Southern blot ... Obtaining complete fragmentation of your DNA at the intended restriction enzyme sites is a critical step in Southern blot ...
more infohttps://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/nucleic-acid-gel-electrophoresis/southern-blotting.html

Southern blotting - Biology-OnlineSouthern blotting - Biology-Online

And a western blot is the same but with protein (and Antibodies instead of a DNA probe). As a side note, Southern is the name ... DNA collected from a cell thought to contain the gene, then underwent the Southern blotting procedure. One of the fragments of ... Southern blotting. Discussion of all aspects of cellular structure, physiology and communication. ... I have heared of southern blotting for school but whats northern. eg how is it done, what is required? ...
more infohttps://www.biology-online.org/biology-forum/viewtopic.php?t=2048

Southern Blot | Definition of Southern Blot by Merriam-WebsterSouthern Blot | Definition of Southern Blot by Merriam-Webster

... a blot consisting of a nitrocellulose or nylon sheet containing spots of DNA for identification by a suitable molecular probe. ... Southern Baptist southern black haw southern blight Southern blot southern blue gum Southern bright southern buckthorn ... Share Southern blot Post the Definition of Southern blot to Facebook Share the Definition of Southern blot on Twitter ... Rhyming Dictionary: Words that rhyme with Southern blot. Comments on Southern blot What made you want to look up Southern blot ...
more infohttps://www.merriam-webster.com/dictionary/Southern%20blot

Southern Blotting | National DiagnosticsSouthern Blotting | National Diagnostics

Southern Blotting: The Procedure. The protocols given are for Genomic Southern Blotting, one of the most sensitive Southern ... Southern Blots will need to be exposed between intensifying screens for up to 96 hours to achieve maximum sensitivity. ... Incomplete digests are the primary cause of Southern Blot failure. It is particularly important to ensure that DNA has fully ... Wrap stripped blot in plastic wrap and place on film overnight to convirm probe removal. Note: If blot is allowed to dry with ...
more infohttps://www.nationaldiagnostics.com/electrophoresis/article/southern-blotting

Southern Blot problems - Molecular BiologySouthern Blot problems - Molecular Biology

Southern Blot problems - Help needed to fix southern problem!! (Oct/05/2006 ). Hi,. I have completed many many southern blots ... I blot using skimmed milk and dextran sulphate with 2 x SSPE and 1% SDS. I tried removing the dextran, it slightly improved but ...
more infohttp://www.protocol-online.org/biology-forums/posts/20849.html

DIG southern blot - Molecular Biology - BioForumDIG southern blot - Molecular Biology - BioForum

DIG southern blot - posted in Molecular Biology: Please can someone give me some suggestions as to why my DIG SB is not working ... DIG southern blot. Started by cbrossin, Jun 03 2011 08:29 AM ... Then do a dot blot of serial dilutions of your DNA (no gel, no ... Then do a dot blot of serial dilutions of your DNA (no gel, no blotting) and hybridize with your probe to establish that the ... blotting) and hybridize with your probe to establish that the probe and DNA hybridize and can be detected. This also ...
more infohttp://www.protocol-online.org/forums/topic/21471-dig-southern-blot/

DNA blot (Southern) - OpenWetWareDNA blot (Southern) - OpenWetWare

The DNA blot or Southern blot is a molecular biology method to probe the genome for the location of a DNA sequence. Genomic DNA ... DNA blot (Southern). From OpenWetWare. Revision as of 06:12, 16 April 2009 by Jakob Suckale. (talk , contribs) (lab-specific ... After size separation DNA pieces are blotted onto a membrane and then hybridised with a labelled nucleic acid probe. The ... Retrieved from "https://openwetware.org/mediawiki/index.php?title=DNA_blot_(Southern)&oldid=302398" ...
more infohttps://openwetware.org/wiki/?title=DNA_blot_

PerfectHyb™ Plus Hybridization Buffer for Northern and Southern blotting, solution | Sigma-AldrichPerfectHyb™ Plus Hybridization Buffer for Northern and Southern blotting, solution | Sigma-Aldrich

Suitable for use in hybridization buffers used in Northern blot, Southern blot, colony/plaque blots, and dot blots. ... Intro to Southern & Northern Blotting , Northern & Southern Blot Protocols The transfer of macromolecules such as nucleic acids ... Buffer / Buffer Salts, Buffers for Hybridization, Core Bioreagents, Life Science Reagents for Northern and Southern Blotting, ... Sigmas PerfectHyb Plus is a ready-to-use hybridization buffer for Northern and Southern blot protocols. Use of PerfectHyb Plus ...
more infohttps://www.sigmaaldrich.com/catalog/product/sigma/h7033?lang=en®ion=US

Analysis of De Novo Telomere Addition by Southern Blot | SpringerLinkAnalysis of De Novo Telomere Addition by Southern Blot | SpringerLink

De novo telomere HO endonuclease S. cerevisiae Southern blot Telomerase This is a preview of subscription content, log in to ... Bonetti D., Longhese M.P. (2018) Analysis of De Novo Telomere Addition by Southern Blot. In: Muzi-Falconi M., Brown G. (eds) ...
more infohttps://link.springer.com/protocol/10.1007%2F978-1-4939-7306-4_25

ASMscience | Southern BlottingASMscience | Southern Blotting

A complementary fragment of DNA is used to detect specific DNA sequences immobilized onto a membrane from a Southern blot. Two ... Long, relatively intact pieces of DNA are required for restriction enzyme digestion for Southern blot analysis of human genomic ... Southern blotting is the process of transferring and immobilizing DNA onto a membrane after restriction enzyme digestion and ... Section 4 : Southern Blotting Authors: Judith A. Scheppler1, Patricia E. Cassin2, Rosa M. Gambier3 VIEW AFFILIATIONS HIDE ...
more infohttp://www.asmscience.org/content/book/10.1128/9781555818135.chap4

PPT - SOUTHERN BLOTTING PowerPoint presentation | free to download  - id: ad542-Y2IwNPPT - SOUTHERN BLOTTING PowerPoint presentation | free to download - id: ad542-Y2IwN

The Southern blot is used to detect the presence of a particular piece of DNA in ... 6. Block with excess DNA. 7. Wash off ... Southern Blot - Southern Blot By: Jacqueline Jai Southern Blot Southern Blot-a piece nitrocellulose paper containing spots of ... Southern Blotting DNA Fingerprinting - Southern Blotting DNA Fingerprinting Southern Blot A Southern Blot identifies specific ... Southern, Northern and Western blotting - Southern, Northern and Western blotting * SOUTHERN BLOTTING The Random Hexamer ...
more infohttp://www.powershow.com/view/ad542-Y2IwN/SOUTHERN_BLOTTING_powerpoint_ppt_presentation

Nachweis von TEL-Genrekombinationen mittels Southern Blot bei Kindern mit akuter lymphoblastischer LeukämieNachweis von TEL-Genrekombinationen mittels Southern Blot bei Kindern mit akuter lymphoblastischer Leukämie

The detection was done due to a new developed non-radioactive Southern blotting with a Digoxigenin marked template. We could ... Das in der vorliegenden Arbeit vorgestellte Verfahren der nicht-radioaktiven Southern Blot Hybridisierung unter Verwendung ...
more infohttps://edoc.hu-berlin.de/handle/18452/15543?C=N

Southern blot analysis and quantitation of PCR amplifie | Open-iSouthern blot analysis and quantitation of PCR amplifie | Open-i

A) A representative blot for the amplification of the VκI family is shown ... Southern blot analysis and quantitation of PCR amplified Vκ recombination products. ( ... Figure 1: Southern blot analysis and quantitation of PCR amplified Vκ recombination products. (A) A representative blot for the ... Figure 1: Southern blot analysis and quantitation of PCR amplified Vκ recombination products. (A) A representative blot for the ...
more infohttps://openi.nlm.nih.gov/detailedresult.php?img=PMC2195934_JEM010340.f1b&req=4

Blotting - Western / N / S > Southern Blotting  Laboratory Product Directory and Equipment Reviews on...Blotting - Western / N / S > Southern Blotting Laboratory Product Directory and Equipment Reviews on...

Southern Blotting products in the SelectScience products and suppliers directory ... Read reviews and compare manufacturers of Blotting - Western / N / S > ... These Capillary Blotting systems offer a contained unit for southern and northern blotting. These units incorporate optimized ... Northern & Southern Blots Hybridization Agilent Technologies. MiracleHyb High-Performance Hybridization Solution QuikHyb ...
more infohttp://www.selectscience.net/blotting---western-+-n-+-s/product-directory/southern-blotting/?catID=1535&u=F33BA311-66C8-47E5-AB3A-DAFF13E5CEFB

Southern blot analysis of transgenic pigs. a, Tail geno | Open-iSouthern blot analysis of transgenic pigs. a, Tail geno | Open-i

Southern blot analysis of transgenic pigs. a, Tail genomic DNA of group I and II F0 generation animals digested with Bgl I. b, ... Figure 3: Southern blot analysis of transgenic pigs. a, Tail genomic DNA of group I and II F0 generation animals digested with ... Figure 3: Southern blot analysis of transgenic pigs. a, Tail genomic DNA of group I and II F0 generation animals digested with ... The numbers in bold indicate positive detection on the blot. Mentions: For this study, Duroc, Yorkshire, and Landrace female ...
more infohttps://openi.nlm.nih.gov/detailedresult.php?img=PMC113740_1472-6750-2-5-3&req=4

Southern blotSouthern blot

... 1. Photograph gel.. 2. Treat gel with 2 volumes of 0.2 N HCl and shake 15 min to depurinate DNA. Pour off ... 8. Blot membrane dry and place in glass cylinder preheated to 65oC. Add FBI hybridization buffer (1.5X SSPE, 10% PEG, 7% SDS) ...
more infohttp://labs.icahn.mssm.edu/sorianolab/protocols/southern-blot/

Duck hepatitis B virus DNA in liver, spleen, and pancreas: analysis by in situ and Southern blot hybridization.  - PubMed - NCBIDuck hepatitis B virus DNA in liver, spleen, and pancreas: analysis by in situ and Southern blot hybridization. - PubMed - NCBI

Duck hepatitis B virus DNA in liver, spleen, and pancreas: analysis by in situ and Southern blot hybridization.. Jilbert AR, ... were examined for the presence of replicative levels of DHBV DNA by in situ and Southern blot hybridization. Hepatocytes, ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/3590623?dopt=Abstract

Final Review - Definition Polycomb regulation DNA methylation Restriction enzymes/southern blot Bisulfite sequencing Genomic...Final Review - Definition Polycomb regulation DNA methylation Restriction enzymes/southern blot Bisulfite sequencing Genomic...

Definition Polycomb regulation DNA methylation Restriction enzymes/southern blot Bisulfite sequencing Genomic imprinting ICR/ ... Unformatted text preview: Definition Polycomb regulation DNA methylation Restriction enzymes/southern blot Bisulfite sequencing ...
more infohttps://www.coursehero.com/file/5455236/Final-Review/

Parity among the randomly amplified polymorphic DNA method, multilocus enzyme electrophoresis, and Southern blot hybridization...Parity among the randomly amplified polymorphic DNA method, multilocus enzyme electrophoresis, and Southern blot hybridization...

Parity among the randomly amplified polymorphic DNA method, multilocus enzyme electrophoresis, and Southern blot hybridization ... Parity among the randomly amplified polymorphic DNA method, multilocus enzyme electrophoresis, and Southern blot hybridization ... Parity among the randomly amplified polymorphic DNA method, multilocus enzyme electrophoresis, and Southern blot hybridization ... Parity among the randomly amplified polymorphic DNA method, multilocus enzyme electrophoresis, and Southern blot hybridization ...
more infohttps://jcm.asm.org/content/35/9/2348?ijkey=3bbdf7bee11705f9684ae9914c190b6cdd1b1d63&keytype2=tf_ipsecsha

Comparison of Southern blot analysis with isotopic and nonisotopic in situ hybridization for the detection of human...Comparison of Southern blot analysis with isotopic and nonisotopic in situ hybridization for the detection of human...

22 cases of invasive squamous cell carcinoma of the uterine cervix were analyzed by Southern blot analysis and in situ ... Blotting, Southern*. Carcinoma / genetics*, pathology. DNA, Neoplasm / analysis. DNA, Viral / analysis*. Female. Humans. ... Comparison of Southern blot analysis with isotopic and nonisotopic in situ hybridization for the detection of human ... Results showed that in situ hybridization performed with isotopic probes is as sensitive as Southern blot analysis and is more ...
more infohttp://www.biomedsearch.com/nih/Comparison-Southern-blot-analysis-with/1315439.html

Characterization of Mycobacterium tuberculosis strains from Vietnamese patients by Southern blot hybridization. - Semantic...Characterization of Mycobacterium tuberculosis strains from Vietnamese patients by Southern blot hybridization. - Semantic...

A total of 41 Mycobacterium tuberculosis strains from patients of Vietnamese origin were analyzed by Southern blot ... Differentiation of Mycobacterium tuberculosis strains by use of a nonradioactive Southern blot hybridization method.. *B ... A total of 41 Mycobacterium tuberculosis strains from patients of Vietnamese origin were analyzed by Southern blot ... Characterization of Mycobacterium tuberculosis strains from Vietnamese patients by Southern blot hybridization.. @article{ ...
more infohttps://www.semanticscholar.org/paper/Characterization-of-Mycobacterium-tuberculosis-from-Yuen-Ross/5e1dceaaf47f6067c07dec7f56cf4d648006813a

Mutation detection by Southern blotting. - MyScienceWorkMutation detection by Southern blotting. - MyScienceWork

Mutation detection by Southern blotting.: Following the discovery of the structure of DNA in 1953, it became clear that ... Nevertheless, more than 30 years after its invention, the Southern blot remains a cornerstone of molecular biology. ... In 1975, Edward Southern published details of a new method for detecting DNA fragments based upon their specific sequence [ ... western and northern blot). The simplicity and effectiveness of the technique led to its universal acceptance as a standard ...
more infohttps://www.mysciencework.com/publication/show/mutation-detection-southern-blotting-c5ff931a
  • The key details related to Southern Blotting Instrument industry like the product definition, cost, variety of applications, demand and supply statistics are covered in this report. (newzy.net)
  • The other blotting techniques emerged from this method have been termed as Northern (for RNA), Western (for proteins), Eastern (for post-translational protein modifications) and Southwestern (for DNA-protein interactions) blotting. (sigmaaldrich.com)
  • These large regions were termed "ultra-long" telomeres in the literature when they were identified using southern blotting and "mega-telomeres" when identified by cytogenetic methods. (wikipedia.org)
  • The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. (sigmaaldrich.com)
  • Das in der vorliegenden Arbeit vorgestellte Verfahren der nicht-radioaktiven Southern Blot Hybridisierung unter Verwendung einer Digoxigenin Markierung hat sich für die Darstellung von Rekombinationen im TEL-Genlokus genomischer DNA als sensitive Vergleichsmethode bewiesen. (hu-berlin.de)
  • The Report entitled Global Southern Blotting Instrument Market 2017 analyses the important factors of the Southern Blotting Instrument market based on present industry situations, market demands, business strategies utilized by Southern Blotting Instrument market players and their growth synopsis. (newzy.net)
  • This report divides the Southern Blotting Instrument market based on the key players, Type, Application and Regions are Mentioned Bellow. (newzy.net)