A 55-kilodalton immunodominant antigen of Porphyromonas gingivalis W50 has arisen via horizontal gene transfer. (1/18383)

A 55-kDa outer membrane protein of Porphyromonas gingivalis W50 is a significant target of the serum immunoglobulin G antibody response of periodontal disease patients and hence may play an important role in host-bacterium interactions in periodontal disease. The gene encoding the 55-kDa antigen (ragB, for receptor antigen B) was isolated on a 9.5-kb partial Sau3AI fragment of P. gingivalis W50 chromosomal DNA in pUC18 by immunoscreening with a monoclonal antibody to this antigen. The 1.6-kb open reading frame (ORF) encoding RagB was located via subcloning and nested-deletion analysis. Sequence analysis demonstrated the presence of an upstream 3.1-kb ORF (ragA) which is cotranscribed with ragB. A number of genetic characteristics suggest that the ragAB locus was acquired by a horizontal gene transfer event. These include a significantly reduced G+C content relative to that of the P. gingivalis chromosome (42 versus 48%) and the presence of mobility elements flanking this locus in P. gingivalis W50. Furthermore, Southern blotting and PCR analyses showed a restricted distribution of this locus in laboratory and clinical isolates of this bacterium. The association of ragAB+ P. gingivalis with clinical status was examined by PCR analysis of subgingival samples. ragAB+ was not detected in P. gingivalis-positive shallow pockets from periodontal disease patients but was present in 36% of the P. gingivalis-positive samples from deep pockets. These data suggest that the ragAB locus was acquired by certain P. gingivalis strains via horizontal gene transfer and that the acquisition of this locus may facilitate the survival of these strains at sites of periodontal destruction.  (+info)

Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase. (2/18383)

A human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization.  (+info)

Mechanisms related to [18F]fluorodeoxyglucose uptake of human colon cancers transplanted in nude mice. (3/18383)

[18F]Fluorodeoxyglucose ([18F]FDG), a glucose analogue, has been widely used for tumor imaging. To investigate the mechanisms related to [18F]FDG uptake by tumors, an experiment involving nude mice was performed. METHODS: Human colon cancer cell lines SNU-C2A, SNU-C4 and SNU-C5 were transplanted to nude mice. Using immunohistochemical staining and Western blot, the expression of glucose transporter (Glut) isoforms (Glut-1 through -5) in xenografted tumors was analyzed. For the analysis of messenger ribonucleic acid (mRNA) expression, reverse-transcription polymerase chain reaction and Northern blot were used and the enzyme activity of hexokinase in cancer tissues was measured by continuous spectrophotometric rate determination. RESULTS: [18F]FDG uptake in SNU-C4 and SNU-C5 cells was higher than in normal colon cells. Among these cells and xenografted tumors, SNU-C5 showed the highest level of [18F]FDG uptake, followed by SNU-C4 and SNU-C2A. An immunostaining experiment showed intense staining of Glut-1 in SNU-C5 tumors but somewhat faint staining in SNU-C4. SNU-C5 tumors also showed positive staining with Glut-3, although this was not the case with SNU-C2A and SNU-C4. Western blot analysis showed the expression of Glut-1 and Glut-3 in all tumors. Experiments involving Northern blot analysis and reverse-transcription polymerase chain reaction confirmed the overexpression of Glut-1 mRNA in all tumors, with the highest level in SNU-C5. The level of Glut-3 mRNA was also elevated in SNU-C5 tumors but not in SNU-C2A and SNU-C4. The enzyme activity of hexokinase did not vary among different tumors. CONCLUSION: Gluts, especially Glut-1, are responsible for [18F]FDG uptake in a nude mouse model of colon cancer rather than hexokinase activity. Increased numbers of glucose transporters at the plasma membrane of cancer cells is attributed to an increased level of transcripts of glucose transporter genes and may be a cause of increased [18F]FDG uptake, at least in colon cancer tumors.  (+info)

Cloning of the peroxiredoxin gene family in rats and characterization of the fourth member. (4/18383)

Peroxiredoxin (PRx) exhibits thioredoxin-dependent peroxidase activity and constitutes a family of proteins. Four members of genes from rat tissues were isolated by PCR using degenerated primers based on the sequences which encode a pair of highly conserved Cys-containing domains, and were then cloned to full-length cDNAs. These included two genes which have previously been isolated in rats, PRx I and PRx II, and two rat homologues of PRx III and PRx IV. We showed, for the first time, the simultaneous expression of all four genes in various rat tissues by Northern blotting. Since a discrepancy exists regarding cellular distribution, we further characterized PRx IV by expressing it in COS-1 cells. This clearly demonstrates that PRx IV is a secretory form and functions within the extracellular space.  (+info)

Cloning of a bovine orphan transporter and its short splicing variant. (5/18383)

We have isolated a cDNA (bv7-3) encoding a member of the Na+,Cl(-)-dependent transporter family and its short splicing variant (bv7-3s) by screening a bovine retina cDNA library. Sequence analysis revealed that bv7-3 encodes a protein of 729 amino acids and is a bovine homologue of the rat orphan transporter v7-3-2. bv7-3s contains 265 amino acids, sharing 252 N-terminal amino acids with bv7-3. Both mRNAs for bv7-3 and bv7-3s were detected in nervous system by Northern blot analysis. In immunofluorescence analysis in transfected HEK 293T cells, myc-tagged bv7-3 was mainly detected on the plasma membrane, whereas myc-tagged bv7-3s showed a pattern of intracellular membrane staining.  (+info)

The latrophilin family: multiply spliced G protein-coupled receptors with differential tissue distribution. (6/18383)

Latrophilin is a brain-specific Ca2+-independent receptor of alpha-latrotoxin, a potent presynaptic neurotoxin. We now report the finding of two novel latrophilin homologues. All three latrophilins are unusual G protein-coupled receptors. They exhibit strong similarities within their lectin, olfactomedin and transmembrane domains but possess variable C-termini. Latrophilins have up to seven sites of alternative splicing; some splice variants contain an altered third cytoplasmic loop or a truncated cytoplasmic tail. Only latrophilin-1 binds alpha-latrotoxin; it is abundant in brain and is present in endocrine cells. Latrophilin-3 is also brain-specific, whereas latrophilin-2 is ubiquitous. Together, latrophilins form a novel family of heterogeneous G protein-coupled receptors with distinct tissue distribution and functions.  (+info)

Glutathione-independent prostaglandin D2 synthase in ram and stallion epididymal fluids: origin and regulation. (7/18383)

Microsequencing after two-dimensional electrophoresis revealed a major protein, glutathione-independent prostaglandin D2 synthase (PGDS) in the anterior epididymal region fluid of the ram and stallion. In this epididymal region, PGDS was a polymorphic compound with a molecular mass around 30 kDa and a range of pI from 4 to 7. PGDS represented 15% and 8% of the total luminal proteins present in this region in the ram and stallion, respectively. The secretion of the protein as judged by in vitro biosynthesis, and the presence of its mRNA as studied by Northern blot analysis, were limited to the proximal caput epididymidis. Using a specific polyclonal antibody raised against a synthetic peptide, PGDS was found throughout the epididymis, decreasing in concentration toward the cauda region. PGDS was also detected in the testicular fluid and seminal plasma by Western blotting. Castration and efferent duct ligation in the ram led to a decrease in PGDS mRNA and secretion. PGDS mRNA was not detected in the stallion 1 mo after castration, and it was restored by testosterone supplementation. This study showed that PGDS is present in the environment of spermatozoa throughout the male genital tract. Its function in the maturation and/or protection of spermatozoa is unknown.  (+info)

Down-regulation of oxytocin-induced cyclooxygenase-2 and prostaglandin F synthase expression by interferon-tau in bovine endometrial cells. (8/18383)

Oxytocin (OT) is responsible for the episodic release of luteolytic prostaglandin (PG) F2alpha from the uterus in ruminants. The attenuation of OT-stimulated uterine PGF2alpha secretion by interferon-tau (IFN-tau) is essential for prevention of luteolysis during pregnancy in cows. To better understand the mechanisms involved, the effect of recombinant bovine IFN-tau (rbIFN-tau) on OT-induced PG production and cyclooxygenase-2 (COX-2) and PGF synthase (PGFS) expression in cultured endometrial epithelial cells was investigated. Cells were obtained from cows at Days 1-3 of the estrous cycle and cultured to confluence in RPMI medium supplemented with 5% steroid-free fetal calf serum. The cells were then incubated in the presence or absence of either 100 ng/ml OT or OT+100 ng/ml rbIFN-tau for 3, 6, 12, and 24 h. OT significantly increased PGF2alpha and PGE2 secretion at all time points (p < 0.01), while rbIFN-tau inhibited the OT-induced PG production and reduced OT receptor binding in a time-dependent manner. OT increased the steady-state level of COX-2 mRNA, measured by Northern blot, which was maximal at 3 h (9-fold increase) and then decreased with time (p < 0.01). OT also caused an increase in COX-2 protein, which peaked at 12 h (11-fold increase), as measured by Western blot. Addition of rbIFN-tau suppressed the induction of COX-2 mRNA (89%, p < 0.01) and COX-2 protein (50%, p < 0.01) by OT. OT also increased PGFS mRNA, and this stimulation was attenuated by rbIFN-tau (p < 0.01). To ensure that the decrease in COX-2 was not solely due to down-regulation of the OT receptor, cells were stimulated with a phorbol ester (phorbol 12-myristate 13-acetate; PMA) in the presence and absence of rbIFN-tau. The results showed that rbIFN-tau also decreased PMA-stimulated PG production and COX-2 protein. It can be concluded that rbIFN-tau inhibition of OT-stimulated PG production is due to down-regulation of OT receptor, COX-2, and PGFS.  (+info)

Western blot Eastern blot Northwestern blot Far-eastern blot Far-western blot Differential display Gilbert, S. F. (2000) ... Northern blotting takes its name from its similarity to the first blotting technique, the Southern blot, named for biologist ... However, the entire process is commonly referred to as northern blotting. The northern blot technique was developed in 1977 by ... while northern blotting is usually looking at one or a small number of genes. A problem in northern blotting is often sample ...
... , much like the northern blot upon which it is based, is used to determine levels of gene expression in ... While northern blot or q-PCR are often used to confirm results, both techniques have drawbacks. Northern blot is limited by its ... In comparison to the Northern blot, the reverse northern blot is able to probe a large number of transcripts at once with less ... Gene expression Northern blot RNA Seq Southern blot Primrose, Sandy B.; Twyman, Richard (2009-04-01). Principles of Genome ...
Similarly, detection of RNA is termed as northern blot. The term "western blot" was given by W. Neal Burnette in 1981, although ... Biology portal Technology portal Chemistry portal Eastern blot Far-eastern blot Far-western blot Fast parallel proteolysis ... diffusion blotting, single-cell resolution western blotting, and automated microfluidic western blotting. Researchers use ... However, NC is brittle, and does not allow the blot to be used for re-probing, whereas PVDF membrane allows the blot to be re- ...
Southern blot for DNA northern blot for RNA reverse northern blot for RNA western blot for proteins far-western blot for ... The reverse northern blot differs from both northern and Southern blot in that DNA is first immobilized on a blotting matrix ... The northern blot is for the detection of specific RNA sequences in complex samples. Northern blotting first separates samples ... making the Far-Western blot a specific case of the Western blot. A southwestern blot is based on Southern blot and is used to ...
Southern blot Northern blot Western blot Southwestern blot "Test Blots, Slot Blots & Dot Blots - Immunodetection , Bio-Rad". ... Performing a dot blot is similar in idea to performing a western blot, with the advantage of faster speed and lower cost. Dot ... A dot blot (or slot blot) is a technique in molecular biology used to detect proteins. It represents a simplification of the ... A general dot blot protocol involves spotting 1-2 microliters of a samples onto a nitrocellulose or PVDF membrane and letting ...
"Northern Blotting". www.lifetechnologies.com. Retrieved 2016-11-26. Wang, X., Seed, B. (2003). A PCR primer bank for ... researchers have used different techniques such as the Northern Blot technique and Real-Time PCR. From these studies, it was ...
Northern blotting; real-time PCR; in situ hybridization; functional analysis; SNP detection and use as antigens and many others ...
Patricia Thomas, developer of the RNA blot which then became known as the northern blot, actually did not use the term. Named ... The northern blot is used to study the presence of specific RNA molecules as relative comparison among a set of different ... A western blot is a technique by which specific proteins can be detected from a mixture of proteins. Western blots can be used ... Josefsen K, Nielsen H (2011). "Northern Blotting Analysis". In Nielsen H (ed.). RNA. Methods in Molecular Biology. Vol. 703. ...
Southern blot Western blot Northern blot Southwestern blot Eastern blot Gel electrophoresis SDS-PAGE Chromatography ... These techniques include the western blot (protein detection), the northern blot (RNA detection), the southwestern blot (DNA- ... Northwestern Blot of Protein-RNA Interaction from Young Rice Panicles RNA Isolation and Northern Blot Analysis Protein Blotting ... is a hybrid analytical technique of the western blot and the northern blot, and is used in molecular biology to detect ...
Other blotting methods (i.e., western blot, northern blot, eastern blot, southwestern blot) that employ similar principles, but ... acids Restriction fragment Genetic fingerprint Northern blot Western blot Eastern blot Southwestern blot Northwestern blot " ... The names for other blotting methods may follow this convention, by analogy. Southern invented Southern blot after combining ... Southern blot is a time consuming method and can only estimate the size of the DNA since it is a semi-quantitative method. It ...
x (2nd ed.). Viking Society for Northern Research. pp. 30-33. ISBN 978-0-903521-27-7. "A time for Blot". Reviving the Elder Way ... a-time-for-blot- Knowst How to Blot Definition of Blót - Zöega dict. Blót explained according to Heithni (CS1 German-language ... Hence it is not on 12 January in the current calendar.) Þorrablót (Iceland) Freyr blot (Sweden): The Óláfs saga Tryggvasonar en ... ISBN 3-533-04230-8. Näsström, Britt-Mari (Oslo 2001). Blot: Tro og offer i det førkristne Norden. ISBN 82-530-2146-1. Orel, ...
Northern blot analysis detected 3 transcripts; a 1-kb transcript was predominant in heart, and a 3-kb transcript was ...
Northern blot analysis documented the samples. The suggested relation to metabolism and vascular disease was attributed to ...
Northern blot analysis also indicated this RNA undergoes specific cleavage processing. The GlmZ sRNA has been shown to ... The expression of SraJ was experimentally confirmed by Northern blotting. This ncRNA is expressed in early logarithmic phase, ...
Northern blotting is a laboratory technique that produces similar information. It is slower and less quantitative, but also ...
The stability and abundance of the shorter 3' transcript is confirmed in both Northern blotting and deep sequencing analysis. ... The expression of SraH was experimentally confirmed by Northern blotting. Its expression is highly abundant in stationary ...
Using Northern Blot Analysis, Vitelli et al. (1996) found that RAB7a was expressed as 1.7- and 2.5-kb transcripts in all cell ...
12 of them were confirmed by Northern blot. DicF RNA DsrA RNA FnrS RNA GadY RNA GcvB RNA IsrJ RNA MicA RNA / SraD RNA MicC RNA ...
The eastern blot is mentioned in an immunology textbook which compares the common blotting methods (Southern, northern and ... Western blot Northwestern blot Far-eastern blot Blot Freeze, HH (1993). "Preparation and analysis of glycoconjugates". Current ... Since this is essentially a western blot, the charge reversal was used to dub this method an eastern blot. (2005) Eastern blot ... distinguishing them from a standard far-western blot. In principle, eastern blotting is similar to lectin blotting (i.e., ...
"In this age of leftist illiberalism, I'm standing up for the truth". The Jewish News of Northern California. May 7, 2021. ... Pollitt, Katha (June 23, 1997). "'Lipstick Blots' on The Feminist Debate". The Washington Post. Suqi, Rima (September 25, 2013 ...
The expression of novel ncRNA of interest was confirmed by northern blotting. In this large scale screen these ncRNAs were ...
The transcription of sRNAs was confirmed by Northern blot. Fur binding was demonstrated to each sRNA promoter, and that ...
U45 was originally cloned from HeLa cells and expression verified by northern blotting. It is related to the snoRNA (MBII-401) ...
U44 was originally cloned from HeLa cells and expression verified by northern blotting. It is predicted to guide 2'O-ribose ...
Expression of 11 of them was confirmed by Northern blot. The sRNAs were shown to be only present in Streptomyces species. sRNA ...
Livy wrote that "the Aequian name was almost blotted out." Still, in 304 BC, the Sabellian peoples of modern northern Abruzzo, ... The northern part of this area was inhabited by the Sidicini, the Aurunci, and the Ausoni (a subgroup of the Aurunci). The ... Barbatus was engaged in a battle near Volterrae (in northern Etruria) which was interrupted by sunset. The Etruscans retreated ... 324 BC - First Roman operations in Daunia, northern Apulia. 321 BC - Roman humiliation at the Caudine Forks. 320 BC - ...
This snoRNA was originally cloned from HeLa cells and expression verified by northern blotting. It is predicted to guide 2'O- ...
This snoRNA was originally cloned from HeLa cells and expression verified by northern blotting. It is predicted to guide 2'O- ...
This snoRNA was originally cloned from HeLa cells and experimentally verified by northern blotting. SNORD48 is predicted to ...
The sRNAs were characterised through RACE mapping and Northern blot experiments. Secondary structures of the sRNAs were ...
Western blot Eastern blot Northwestern blot Far-eastern blot Far-western blot Differential display Gilbert, S. F. (2000) ... Northern blotting takes its name from its similarity to the first blotting technique, the Southern blot, named for biologist ... However, the entire process is commonly referred to as northern blotting. The northern blot technique was developed in 1977 by ... while northern blotting is usually looking at one or a small number of genes. A problem in northern blotting is often sample ...
Scientific Video Article | والهدف من هذا المنشور هو تصور ومناقشة الخطوات المنطوق لتعزيز بروتوكول الشمالية صمة عار على...
Analysis of RNA by Northern Blotting * c-Kit-mediated overlapping and unique functional and biochemical outcomes via diverse ... Analysis of RNA by Northern Blotting * c-Kit-mediated overlapping and unique functional and biochemical outcomes via diverse ... Northern hybridization is used to measure the quantity and dimension of RNAs transcribed from eukaryotic genes and to estimate ... Category: Northern Blotting. Analysis of RNA by Northern Blotting. *Antibodies. *Biochemical Kits ...
Northern blotting. RPE1 cells were transduced with AAVS1 or NHT gRNA expressing constructs (SpCas9 NHT, enAsCas12a NHT, SpCas9 ... 2g). We then performed Northern Blotting to quantify RNA processing of gRNAs derived from the CHyMErA plasmid. This revealed a ... In line with this, Northern Blotting again revealed a difference between RNA processing of gRNAs derived from CHyMErA.v2 or ... For northern blotting analysis, 5 μg of each RNA were mixed with 2 × GLII loading buffer (NEB, B0363A), boiled at 95 °C for 5 ...
Title: Northern blot. Keywords: Northern blot illustration figure drawing diagram image. This illustration is included in the ...
Northern blotting analysis of the tsRNAs derived from tRNA-GlyGCC (A) and tRNA-GluCTC (B), after 1-h incubation without (-ANG) ... Northern blotting analysis of the tsRNAs derived from tRNA-GlyGCC (A) and tRNA-GluCTC (B), after 1-h incubation without (-ANG) ... Northern blotting analysis of the tsRNAs derived from tRNA-GlyGCC (A) and tRNA-GluCTC (B), after 1-h incubation without (-ANG) ... Northern blotting analysis of the tsRNAs derived from tRNA-GlyGCC (A) and tRNA-GluCTC (B), after 1-h incubation without (-ANG) ...
Northern Blot Analysis. Overnight yeast cultures were resuspended in fresh YPD media to an OD600 of 0.2 and grown to the ... The same cells were examined by northern blot to quantify the amounts of single tRNAs. Levels of three representative tRNAs: ... Blots were developed using higher-resolution photographic films (Medical X-Ray film blue from AGFA, Morstel, Belgium). RNA ... Determination of specific tRNA levels by northern hybridization with probes specific for mature tRNAiMet, tRNAPhe, and tRNATyr ...
Northern blot analysis. Total RNA was isolated from the brain and retina as above. From the brain total RNA, poly(A)+RNA was ... Northern blot analysis for the ayu rhodopsin mRNA in the retina and brain of ayu. Total RNA (10 μg) from the retina and poly(A) ... Northern blot analysis was first carried out to detect the ayu rhodopsin mRNA in the brain and retina. A single band of ... Southern and Northern blots and reverse-transcription PCR analyses indicate that the same rhodopsin gene is expressed in the ...
Northern Blot Validation of sRNA Expression. We validated a sampling of predicted sRNAs from ANNOgesic by northern blot for E. ... we tested 19 predicted sRNAs by northern blot (Figure 7A). We detected ~74% (14/19) by northern blot; 5 were undetectable. Of ... Northern Blot Analysis. DNase I-treated total RNA (5 µg) was separated on a 6% polyacrylamide gel containing 7M urea. RNA was ... Figure 7 Validation of predicted sRNAs of E. faecalis by Northern blot. Probing of selected E. faecalis candidate sRNAs was ...
Western blot blotting tank SEMIDRY. Contact a supplier or the parent company directly to get a quote or to find out a price or ... All units can be used for all types of blotting: Western, Southern and Northern via uncomplicated buffer and set up procedures ... Rapid transfer times Western, Southern and Northern Blots Economic Transfers due to very low buffer volumes Screw down lid Gels ... Semi Dry Blotting has the added benefit of economic transfers. Due to very low buffer volumes typically only a few millilitres ...
Northern Blotting.. A human multiple tissue Northern blot (CLONTECH, Palo Alto, CA) was used for localization of hOAT1 ... Northern blot analysis was used to examine hOAT1 expression in different human tissues with an hOAT1-specific DNA probe. A ... A, Northern analysis of human tissue-specific poly(A)+ RNA. After hybridization with a 430-bp, 32P-labeled hOAT1 fragment, a ... A cDNA clone was isolated from a human renal library and designated human organic anion transporter 1 (hOAT1). Northern ...
1 Northern blot analysis of various sRNAs in P. laumondii.. Expression of various sRNAs in P. laumondii at different time ... 2 Northern blot analysis of various sRNAs in X. szentirmaii.. Expression of various sRNAs in X. szentirmaii at different time ... The RNA was loaded on northern blots and probed for the indicated sRNAs. Probing for 5 S rRNA served as loading control. ... The RNA was loaded on northern blots and probed for the indicated sRNAs. Probing for 5 S rRNA served as loading control. ...
Blotting, Northern * Brain Chemistry / drug effects* * Haloperidol / pharmacology * Loxapine / pharmacology * Male * ...
Northern blot analysis to determine expression of a) surfactant protein (SP)-A, b) SP-B and c) SP-C mRNA in total cellular RNA ... Northern blot analysis followed, using the complementary DNAs listed below.. Human surfactant protein A, B and C and 18S ... Northern blot analysis of surfactant protein mRNAs. RNA was extracted from freshly purified PLECs or primary PLECs cultured on ... and Western and Northern blot analyses. Electrophoretic mobility shift assays were performed in order to examine nuclear factor ...
Blotting, Northern * Blotting, Western * Cell Division * Colorectal Neoplasms / drug therapy * Colorectal Neoplasms / ...
Northern blots. Request a detailed protocol RNA was purified using TRIzol reagent (Life Technologies) and resuspended in 2 μl ... 1) In Figure 1, the western blots for TERT are done using an antibody that has not been characterized - the Methods simply cite ... Spot-blot hybridization was used to detect hTR. Relative activity and hTR content were normalized to the input or bound sample ... Spot-blot hybridization was used to detect hTR. Relative activity and hTR content were normalized to the WT TERT purification ...
Northern blotting.. Cells were lysed in Trizol and processed according to the manufacturers instructions. Murine tissues were ... C: Lcn2 protein levels in serum from fed male ob/+ (n = 6) and ob/ob (n = 10) mice, measured by Western blotting and expressed ... C: Lcn2 protein levels in serum from fed male ob/+ (n = 6) and ob/ob (n = 10) mice, measured by Western blotting and expressed ... Northern analysis showed that white adipose tissue (WAT) was by far the dominant site of expression of Lcn2 in wild-type male ...
... human 12-lane multiple tissue Northern blot; CLONTECH) was used for the Northern blot analysis for hOAT3. We used full-length ... Northern blot analysis of hOAT3 (A) and β-actin (B). Human multiple tissue blot (CLONTECH) that contains 2 μg of poly(A)+ RNA ... Northern Blot Analysis.. A commercially available hybridization blot containing poly (A)+ RNA from various human tissues ( ... Northern blot analysis revealed that hOAT3 mRNA is expressed in the kidney, brain, and skeletal muscle. When expressed in ...
northern-blotting Unrestricted Use. CC BY Biology Rating 0.0 stars Biology is designed for multi-semester biology courses for ...
RNA and protein-blotting studies. The protocols for RNA isolation and Northern blotting have been described (18). Probes ... Representative autoradiograph of Northern blot analyses performed with total RNA (15 μg/lane) isolated from mouse hearts at ... Representative autoradiograph of Northern blot analyses performed with total RNA isolated from sex-matched, adult littermate ... The autoradiographs shown at the top represent Northern blot studies performed with total RNA isolated from cardiac myocytes ...
Northern blotting and reverse transcriptase PCR (RT-PCR).. RNA for Northern blots was prepared from mid-logarithmic-phase ... Northern blots using the gtcA-internal fragment cloned in pNP1 as probe suggested the presence of a ca. 700-nt transcript (data ... We also thank Nikhat Parveen for assistance with Northern blots, and we are grateful for the valuable participation of Timothy ... Signal intensity was quite low in Northern blots, suggesting a low abundance of the message. RT-PCR was therefore used to ...
It then lies about one-third of the way from the horizon to the zenith from mid-northern latitudes. (The farther south you live ... www.astronomy.com/observing/an-asteroid-will-briefly-blot-out-the-skys-brightest-star-tonight/ An asteroid will briefly blot ... An asteroid will briefly blot out the skys brightest star tonight. Sirius, the Dog Star, is usually the brightest star in the ... an-asteroid-will-briefly-blot-out-the-skys-brightest-star-tonight https:// ...
In vitro luciferase assays, co-immunoprecipitation as well as western blotting experiments were performed to further study the ...
Northern blot analysis. Northern blot analysis of total mouse brain RNA was performed as described previously using a ... 1D). Northern blot analysis of the brains of β1 (+/+), β1 (+/-), and β1 (-/-) mice showed that β1 mRNA is significantly reduced ... E, Northern blot analysis of total brain RNA isolated from β1 (+/+), (+/-), and (-/-) mice, as indicated. Molecular weight ... C, Southern blot analysis of ES cell clones. Digestion with KpnI, followed by Southern blotting with the 5′ probe, yielded a ...
Representative Northern blots of PTEN mRNA (A), PTP1B mRNA (B), and SHIP2 mRNA (C) in livers from db/db mice treated once a ... Representative Northern blots of PTEN mRNA (A), PTP1B mRNA (B), and SHIP2 mRNA (C) in livers from db/db mice treated once a ... Representative Northern blots of PTEN and G3PDH mRNA in fat and muscle from db/db mice treated with saline, 50 mg/kg AS1, or 50 ... Representative Northern blots of PTEN and G3PDH mRNA in fat and muscle from db/db mice treated with saline, 50 mg/kg AS1, or 50 ...
Northern Blot. Northern blot is a laboratory analysis method used to study RNA. Specifically, purified RNA fragments from a ... Southern Blot. Southern blot analysis is a laboratory method used to study DNA. Specifically, purified DNA from a biological ... blot. The method is named for its creator, British molecular biologist Edwin Southern. MORE ...
Northern blot Is the Subject Area "Northern blot" applicable to this article? Yes. No. ...
Northern blot. Western blot. RT-PCR. cDNA clones. RNase protection. Nuclease S1. ...
Northern blotting: Microarrays nucleic acids: DNA or RNA pRNA: Is this prohead, promoter and/or packaging RNA? post- ... Microarrays Northern blotting; Omes & omics ribonome, ribonomics RNA amplification: Amplification of messenger RNA (mRNA). ...
  • The northern blot, or RNA blot, is a technique used in molecular biology research to study gene expression by detection of RNA (or isolated mRNA) in a sample. (wikipedia.org)
  • Northern blot analysis revealed that hOAT3 mRNA is expressed in the kidney, brain, and skeletal muscle. (aspetjournals.org)
  • RNA was extracted from some cells and the extent of expression of inducible NOS mRNA was determined by Northern blotting. (cdc.gov)
  • The predicted size of the COMT transcript was 1.8-2.0 kb, which could be confirmed from Northern hybridization analyses of the isolated rat liver mRNA. (lu.se)
  • Northern blotting involves the use of electrophoresis to separate RNA samples by size, and detection with a hybridization probe complementary to part of or the entire target sequence. (wikipedia.org)
  • Strictly speaking, the term 'northern blot' refers specifically to the capillary transfer of RNA from the electrophoresis gel to the blotting membrane. (wikipedia.org)
  • Southern and Northern blots and reverse-transcription PCR analyses indicate that the same rhodopsin gene is expressed in the retina and the brain but not in the pineal organ of ayu. (bioone.org)
  • These cells were characterised by PCR, immunocytochemistry, electron microscopy, and Western and Northern blot analyses. (ersjournals.com)
  • We performed northern blot analyses for the guide RNA truncations and found increased levels of expression for the longer tracrRNA sequences, suggesting that improved target cleavage was at least partially due to higher sgRNA expression or stability ( Fig. 1c ). (cdc.gov)
  • Northern hybridization is used to measure the quantity and dimension of RNAs transcribed from eukaryotic genes and to estimate their abundance. (humccancer.org)
  • It is also known as Northern hybridization, or RNA hybridization. (asu.edu)
  • Component of hybridization solutions for Northern and Southern blotting. (nationaldiagnostics.com)
  • It is especially designed for use in Southern and Northern Blotting, and in situ hybridization procedures. (nationaldiagnostics.com)
  • Commonly, nucleic acids or proteins are electrophoretically separated on agarose or acrylamide gels and then transferred (blotted) from the gel onto the NC. (cdc.gov)
  • Rapid transfer times Western, Southern and Northern Blots Economic Transfers due to very low buffer volumes Screw down lid Gels from 0.25 up to 10mm thick can be blotted Uniform heat dispersion Long life electrodes OVERVIEW These Semi Dry Blotters offer rapid transfer times for DNA, RNA and protein blotting typically 15 to 30 minutes. (medicalexpo.com)
  • Such terms as 'Southern' (transfer of DNA), 'Northern' (transfer of RNA), and 'Western' (transfer of protein) blots have been coined. (cdc.gov)
  • All units can be used for all types of blotting: Western, Southern and Northern via uncomplicated buffer and set up procedures and are compatible with gel thicknesses from 0.25 up to 10mm without the need for additional equipment. (medicalexpo.com)
  • We used quantitative Western blotting to observe Lcn2 serum levels in lean and obese mouse models. (diabetesjournals.org)
  • ABSTRACT Crude antigenic preparations from Setaria equina were used in ELISA and Western blotting to examine cross- reaction with human sera from areas endemic for bancroftian filariasis. (who.int)
  • ELISA et transfert Western afin d'étudier la réaction croisée avec des sérums humains en provenance de zones endémiques pour la filariose de Bancroft. (who.int)
  • Northern analysis detected a specific 2.5-kilobase pair hOAT1 transcript only in human kidney. (aspetjournals.org)
  • Southern blot analysis of total cell DNA of the transformants shows that the wild type psaC gene has been replaced by the interrupted psaC gene through homologous recombination. (unige.ch)
  • Employing the DNA blot analysis only one COMT-encoding gene was found in the rat genome. (lu.se)
  • A general blotting procedure starts with extraction of total RNA from a homogenized tissue sample or from cells. (wikipedia.org)
  • The procedure for and theory behind Northern blotting is almost identical to that of Southern blotting, except you are working with RNA instead of DNA. (asu.edu)
  • Northern blotting takes its name from its similarity to the first blotting technique, the Southern blot, named for biologist Edwin Southern. (wikipedia.org)
  • The transfer buffer used for the blotting usually contains formamide because it lowers the annealing temperature of the probe-RNA interaction, thus eliminating the need for high temperatures, which could cause RNA degradation. (wikipedia.org)
  • Washing buffer for Northern and Southern blotting. (nationaldiagnostics.com)
  • Since the gels are fragile and the probes are unable to enter the matrix, the RNA samples, now separated by size, are transferred to a nylon membrane through a capillary or vacuum blotting system. (wikipedia.org)
  • A nylon membrane with a positive charge is the most effective for use in northern blotting since the negatively charged nucleic acids have a high affinity for them. (wikipedia.org)
  • With northern blotting it is possible to observe cellular control over structure and function by determining the particular gene expression rates during differentiation and morphogenesis, as well as in abnormal or diseased conditions. (wikipedia.org)
  • Transfer-messenger RNA (tmRNA) expression was recorded for each blot for normalization against internal loading controls. (cdc.gov)
  • Activation of Xwnt11 is induced in an immediate-early fashion by Xbra and its expression in vivo is abolished by a dominant-interfering form of Xbra , Xbra -En(R). Overexpression of a dominant-negative form of Xwnt11 , like overexpression of Xbra -En(R), inhibits convergent extension movements. (xenbase.org)
  • To create controls for comparison in a northern blot, samples not displaying the gene product of interest can be used after determination by microarrays or RT-PCR. (wikipedia.org)
  • The gels can be stained with ethidium bromide (EtBr) and viewed under UV light to observe the quality and quantity of RNA before blotting. (wikipedia.org)
  • Commonly cDNA is created with labelled primers for the RNA sequence of interest to act as the probe in the northern blot. (wikipedia.org)
  • QIAamp technology yields total cellular RNA from fresh whole blood and other sample sources that is ready to use in RT-PCR and blotting procedures. (qiagen.com)
  • NC sheets up to 20 x 20 cm are often used in these blotting procedures. (cdc.gov)
  • Uniform heat dispersion across the blot sandwich ensures stable transfer times and no heat induced sample loss or transfer distortions. (medicalexpo.com)
  • These Semi-Dry Blotters utilise a screw down lid, which secures the blot sandwich and allows complete control of pressure ensuring even transfer. (medicalexpo.com)
  • Being translucent, it allows viewing of the blot sandwich to ensure correct positioning and transfer is occurring correctly. (medicalexpo.com)
  • The northern blot technique was developed in 1977 by James Alwine, David Kemp, and George Stark at Stanford University. (wikipedia.org)
  • This technique came to be known as Southern blotting. (asu.edu)
  • Is the Subject Area "Northern blot" applicable to this article? (plos.org)
  • Instead, it will blot out its center, leaving a ring at the edges. (popsci.com)
  • Notable among these were a study of hard-metal workers in several small factories in northern Italy. (who.int)
  • The lower expression levels of the SCD1 gene in both diabetic groups compared with controls were further confirmed by Northern blot analysis and immunohistochemistry. (diabetesjournals.org)
  • Northern blotting involves the use of electrophoresis to separate RNA samples by size, and detection with a hybridization probe complementary to part of or the entire target sequence. (wikipedia.org)
  • [ 37 ] Northern membrane was probed by using corresponding hybridization fragments of mouse Cx36, mouse Cx45 [ 38 ] and human Cx59 (1.5 kb) as well as human Cx62 (1.6 kb) genomic DNA (this study). (medscape.com)
  • Subsequently, the amounts of total RNA on the Northern blot were standardized by hybridization of to a probe of glyceraldehydephosphate dehydrogenase (GAPDH). (medscape.com)
  • This was analysed by Northern hybridization with a 280 base pair probe containing sequences derived from exons 3 and 4 of the insulin-like growth factor 1 gene. (nih.gov)
  • A cDNA encoding a defensin-like peptide, Coprisin, was isolated from bacteria-immunized dung beetle, Copris tripartitus , by using differential dot blot hybridization. (hindawi.com)
  • In this study, we constructed a full-length cDNA library from bacteria-immunized C. tripartitus and then selected up-regulated clones using differential screening by a dot blot hybridization analysis. (hindawi.com)
  • Coprisin cDNA clones were identified by a dot blot hybridization. (hindawi.com)
  • Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES. (bvsalud.org)
  • Protein expression will be analyzed by immunohistochemistry (for localization) and western blotting (for quantification). (nih.gov)
  • A general blotting procedure starts with extraction of total RNA from a homogenized tissue sample or from cells. (wikipedia.org)
  • The amounts of total RNA on the Northern blot were roughly standardized by determination of the intensities of the ethidium bromide stained 18S- and 28S rRNA. (medscape.com)
  • Since the gels are fragile and the probes are unable to enter the matrix, the RNA samples, now separated by size, are transferred to a nylon membrane through a capillary or vacuum blotting system. (wikipedia.org)
  • A nylon membrane with a positive charge is the most effective for use in northern blotting since the negatively charged nucleic acids have a high affinity for them. (wikipedia.org)
  • Probes for northern blotting are composed of nucleic acids with a complementary sequence to all or part of the RNA of interest. (wikipedia.org)
  • The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. (sigmaaldrich.com)
  • Commonly, nucleic acids or proteins are electrophoretically separated on agarose or acrylamide gels and then transferred (blotted) from the gel onto the NC. (cdc.gov)
  • She has hardware and software which scans the autoradiogram coming out of Northern blot analysis into a TIFF file on her PC. (bio.net)
  • TNF-α gene transcription was monitored by Northern blot analysis. (portlandpress.com)
  • In some experiments, changes in NEP-specific mRNAs in the presence or absence of dexamethasone and/or the inhibitors were also evaluated by Northern blot analysis. (cdc.gov)
  • Northern blot analysis indicated that NEP-specific mRNAs were also increased by dexamethasone. (cdc.gov)
  • Northern blot analysis shows its expression on adult testis, adult heart, and fetal brain. (thermofisher.com)
  • Northern-blot analysis of poly(A)+ RNA from several human tissues indicates a complex expression pattern differing across tissues. (tcdb.org)
  • About 10 years ago, the Maraia Lab developed an assay, based on a northern blot, to identify these types of mutations in tRNA modification enzymes (TME). (nih.gov)
  • Falsified the results for NT2 binding to the Col11a2 DNA sequence in electrophoretic mobility shift assays in Figures 2D and 6B, and falsified the Western blot for NT2 mutant proteins in Figure 8B in MCB 22:4256-4267, 2002. (nih.gov)
  • Western blotting was used to confirm asymptomatic VL ( 8 ). (cdc.gov)
  • Southern blotting was introduced by Edwin Southern in 1975 as a method to detect specific sequences of DNA in DNA samples. (sigmaaldrich.com)
  • Commonly cDNA is created with labelled primers for the RNA sequence of interest to act as the probe in the northern blot. (wikipedia.org)
  • 20X SSC (93017) may be used in place of SSPE in similar blotting protocols. (sigmaaldrich.com)
  • The transfer buffer used for the blotting usually contains formamide because it lowers the annealing temperature of the probe-RNA interaction, thus eliminating the need for high temperatures, which could cause RNA degradation. (wikipedia.org)
  • Our laboratory developed a northern blot method that can quantify relative levels of base modifications on multiple specific tRNAs ∼10 yr ago, which has been used to characterize four different TME deficiencies and is likely further extendable. (nih.gov)
  • HVL has not been reported) and 11 sites (2,086 serum samples) in the HVL-endemic northern region, where the disease was previously reported in 8 of 11 sites ( Figure ). (cdc.gov)
  • Falsified the Northern blots in multiple panels of Figure 3, MCB 20:4428-4435, 2000. (nih.gov)
  • In this study, HVL seroprevalence in northern Israel was compared with that in a region free of clinical disease. (cdc.gov)
  • Your comments on the use of these packages, in light of our Northern blot application, will be of interest to us. (bio.net)
  • Harris, CJ, Baulcombe, DC & Molnar, A 2017, Improved denaturation of small RNA duplexes and it's application for northern blotting . (ed.ac.uk)
  • However, the entire process is commonly referred to as northern blotting. (wikipedia.org)
  • In 1815, Mount Tambora erupted and its emissions spread across the globe, blotting out the sun for almost an entire year. (eschoolnews.com)
  • The major difference is that RNA, rather than DNA, is analyzed in the northern blot. (wikipedia.org)