A 55-kilodalton immunodominant antigen of Porphyromonas gingivalis W50 has arisen via horizontal gene transfer. (1/18383)A 55-kDa outer membrane protein of Porphyromonas gingivalis W50 is a significant target of the serum immunoglobulin G antibody response of periodontal disease patients and hence may play an important role in host-bacterium interactions in periodontal disease. The gene encoding the 55-kDa antigen (ragB, for receptor antigen B) was isolated on a 9.5-kb partial Sau3AI fragment of P. gingivalis W50 chromosomal DNA in pUC18 by immunoscreening with a monoclonal antibody to this antigen. The 1.6-kb open reading frame (ORF) encoding RagB was located via subcloning and nested-deletion analysis. Sequence analysis demonstrated the presence of an upstream 3.1-kb ORF (ragA) which is cotranscribed with ragB. A number of genetic characteristics suggest that the ragAB locus was acquired by a horizontal gene transfer event. These include a significantly reduced G+C content relative to that of the P. gingivalis chromosome (42 versus 48%) and the presence of mobility elements flanking this locus in P. gingivalis W50. Furthermore, Southern blotting and PCR analyses showed a restricted distribution of this locus in laboratory and clinical isolates of this bacterium. The association of ragAB+ P. gingivalis with clinical status was examined by PCR analysis of subgingival samples. ragAB+ was not detected in P. gingivalis-positive shallow pockets from periodontal disease patients but was present in 36% of the P. gingivalis-positive samples from deep pockets. These data suggest that the ragAB locus was acquired by certain P. gingivalis strains via horizontal gene transfer and that the acquisition of this locus may facilitate the survival of these strains at sites of periodontal destruction. (+info)
Tissue specific expression and chromosomal mapping of a human UDP-N-acetylglucosamine: alpha1,3-d-mannoside beta1, 4-N-acetylglucosaminyltransferase. (2/18383)A human cDNA for UDP- N -acetylglucosamine:alpha1,3-d-mannoside beta1,4- N- acetylglucosaminyltransferase (GnT-IV) was isolated from a liver cDNA library using a probe based on a partial cDNA sequence of the bovine GnT-IV. The cDNA encoded a complete sequence of a type II membrane protein of 535 amino acids which is 96% identical to the bovine GnT-IV. Transient expression of the human cDNA in COS7 cells increased total cellular GnT-IV activity 25-fold, demonstrating that this cDNA encodes a functional human GnT-IV. Northern blot analysis of normal tissues indicated that at least five different sizes of mRNA (9.7, 7.6, 5.1, 3.8, and 2.4 kb) forGnT-IV are expressed in vivo. Furthermore, these mRNAs are expressed at different levels between tissues. Large amounts of mRNA were detected in tissues harboring T lineage cells. Also, the promyelocytic leukemia cell line HL-60 and the lymphoblastic leukemia cell line MOLT-4 revealed abundant mRNA. Lastly, the gene was mapped at the locus on human chromosome 2, band q12 by fluorescent in situ hybridization. (+info)
Mechanisms related to [18F]fluorodeoxyglucose uptake of human colon cancers transplanted in nude mice. (3/18383)[18F]Fluorodeoxyglucose ([18F]FDG), a glucose analogue, has been widely used for tumor imaging. To investigate the mechanisms related to [18F]FDG uptake by tumors, an experiment involving nude mice was performed. METHODS: Human colon cancer cell lines SNU-C2A, SNU-C4 and SNU-C5 were transplanted to nude mice. Using immunohistochemical staining and Western blot, the expression of glucose transporter (Glut) isoforms (Glut-1 through -5) in xenografted tumors was analyzed. For the analysis of messenger ribonucleic acid (mRNA) expression, reverse-transcription polymerase chain reaction and Northern blot were used and the enzyme activity of hexokinase in cancer tissues was measured by continuous spectrophotometric rate determination. RESULTS: [18F]FDG uptake in SNU-C4 and SNU-C5 cells was higher than in normal colon cells. Among these cells and xenografted tumors, SNU-C5 showed the highest level of [18F]FDG uptake, followed by SNU-C4 and SNU-C2A. An immunostaining experiment showed intense staining of Glut-1 in SNU-C5 tumors but somewhat faint staining in SNU-C4. SNU-C5 tumors also showed positive staining with Glut-3, although this was not the case with SNU-C2A and SNU-C4. Western blot analysis showed the expression of Glut-1 and Glut-3 in all tumors. Experiments involving Northern blot analysis and reverse-transcription polymerase chain reaction confirmed the overexpression of Glut-1 mRNA in all tumors, with the highest level in SNU-C5. The level of Glut-3 mRNA was also elevated in SNU-C5 tumors but not in SNU-C2A and SNU-C4. The enzyme activity of hexokinase did not vary among different tumors. CONCLUSION: Gluts, especially Glut-1, are responsible for [18F]FDG uptake in a nude mouse model of colon cancer rather than hexokinase activity. Increased numbers of glucose transporters at the plasma membrane of cancer cells is attributed to an increased level of transcripts of glucose transporter genes and may be a cause of increased [18F]FDG uptake, at least in colon cancer tumors. (+info)
Cloning of the peroxiredoxin gene family in rats and characterization of the fourth member. (4/18383)Peroxiredoxin (PRx) exhibits thioredoxin-dependent peroxidase activity and constitutes a family of proteins. Four members of genes from rat tissues were isolated by PCR using degenerated primers based on the sequences which encode a pair of highly conserved Cys-containing domains, and were then cloned to full-length cDNAs. These included two genes which have previously been isolated in rats, PRx I and PRx II, and two rat homologues of PRx III and PRx IV. We showed, for the first time, the simultaneous expression of all four genes in various rat tissues by Northern blotting. Since a discrepancy exists regarding cellular distribution, we further characterized PRx IV by expressing it in COS-1 cells. This clearly demonstrates that PRx IV is a secretory form and functions within the extracellular space. (+info)
Cloning of a bovine orphan transporter and its short splicing variant. (5/18383)We have isolated a cDNA (bv7-3) encoding a member of the Na+,Cl(-)-dependent transporter family and its short splicing variant (bv7-3s) by screening a bovine retina cDNA library. Sequence analysis revealed that bv7-3 encodes a protein of 729 amino acids and is a bovine homologue of the rat orphan transporter v7-3-2. bv7-3s contains 265 amino acids, sharing 252 N-terminal amino acids with bv7-3. Both mRNAs for bv7-3 and bv7-3s were detected in nervous system by Northern blot analysis. In immunofluorescence analysis in transfected HEK 293T cells, myc-tagged bv7-3 was mainly detected on the plasma membrane, whereas myc-tagged bv7-3s showed a pattern of intracellular membrane staining. (+info)
The latrophilin family: multiply spliced G protein-coupled receptors with differential tissue distribution. (6/18383)Latrophilin is a brain-specific Ca2+-independent receptor of alpha-latrotoxin, a potent presynaptic neurotoxin. We now report the finding of two novel latrophilin homologues. All three latrophilins are unusual G protein-coupled receptors. They exhibit strong similarities within their lectin, olfactomedin and transmembrane domains but possess variable C-termini. Latrophilins have up to seven sites of alternative splicing; some splice variants contain an altered third cytoplasmic loop or a truncated cytoplasmic tail. Only latrophilin-1 binds alpha-latrotoxin; it is abundant in brain and is present in endocrine cells. Latrophilin-3 is also brain-specific, whereas latrophilin-2 is ubiquitous. Together, latrophilins form a novel family of heterogeneous G protein-coupled receptors with distinct tissue distribution and functions. (+info)
Glutathione-independent prostaglandin D2 synthase in ram and stallion epididymal fluids: origin and regulation. (7/18383)Microsequencing after two-dimensional electrophoresis revealed a major protein, glutathione-independent prostaglandin D2 synthase (PGDS) in the anterior epididymal region fluid of the ram and stallion. In this epididymal region, PGDS was a polymorphic compound with a molecular mass around 30 kDa and a range of pI from 4 to 7. PGDS represented 15% and 8% of the total luminal proteins present in this region in the ram and stallion, respectively. The secretion of the protein as judged by in vitro biosynthesis, and the presence of its mRNA as studied by Northern blot analysis, were limited to the proximal caput epididymidis. Using a specific polyclonal antibody raised against a synthetic peptide, PGDS was found throughout the epididymis, decreasing in concentration toward the cauda region. PGDS was also detected in the testicular fluid and seminal plasma by Western blotting. Castration and efferent duct ligation in the ram led to a decrease in PGDS mRNA and secretion. PGDS mRNA was not detected in the stallion 1 mo after castration, and it was restored by testosterone supplementation. This study showed that PGDS is present in the environment of spermatozoa throughout the male genital tract. Its function in the maturation and/or protection of spermatozoa is unknown. (+info)
Down-regulation of oxytocin-induced cyclooxygenase-2 and prostaglandin F synthase expression by interferon-tau in bovine endometrial cells. (8/18383)Oxytocin (OT) is responsible for the episodic release of luteolytic prostaglandin (PG) F2alpha from the uterus in ruminants. The attenuation of OT-stimulated uterine PGF2alpha secretion by interferon-tau (IFN-tau) is essential for prevention of luteolysis during pregnancy in cows. To better understand the mechanisms involved, the effect of recombinant bovine IFN-tau (rbIFN-tau) on OT-induced PG production and cyclooxygenase-2 (COX-2) and PGF synthase (PGFS) expression in cultured endometrial epithelial cells was investigated. Cells were obtained from cows at Days 1-3 of the estrous cycle and cultured to confluence in RPMI medium supplemented with 5% steroid-free fetal calf serum. The cells were then incubated in the presence or absence of either 100 ng/ml OT or OT+100 ng/ml rbIFN-tau for 3, 6, 12, and 24 h. OT significantly increased PGF2alpha and PGE2 secretion at all time points (p < 0.01), while rbIFN-tau inhibited the OT-induced PG production and reduced OT receptor binding in a time-dependent manner. OT increased the steady-state level of COX-2 mRNA, measured by Northern blot, which was maximal at 3 h (9-fold increase) and then decreased with time (p < 0.01). OT also caused an increase in COX-2 protein, which peaked at 12 h (11-fold increase), as measured by Western blot. Addition of rbIFN-tau suppressed the induction of COX-2 mRNA (89%, p < 0.01) and COX-2 protein (50%, p < 0.01) by OT. OT also increased PGFS mRNA, and this stimulation was attenuated by rbIFN-tau (p < 0.01). To ensure that the decrease in COX-2 was not solely due to down-regulation of the OT receptor, cells were stimulated with a phorbol ester (phorbol 12-myristate 13-acetate; PMA) in the presence and absence of rbIFN-tau. The results showed that rbIFN-tau also decreased PMA-stimulated PG production and COX-2 protein. It can be concluded that rbIFN-tau inhibition of OT-stimulated PG production is due to down-regulation of OT receptor, COX-2, and PGFS. (+info)
Western blot Eastern blot Northwestern blot Far-eastern blot Far-western blot Differential display Gilbert, S. F. (2000) ... Northern blotting takes its name from its similarity to the first blotting technique, the Southern blot, named for biologist ... However, the entire process is commonly referred to as northern blotting. The northern blot technique was developed in 1977 by ... while northern blotting is usually looking at one or a small number of genes. A problem in northern blotting is often sample ...
Reverse northern blot
... , much like the northern blot upon which it is based, is used to determine levels of gene expression in ... While northern blot or q-PCR are often used to confirm results, both techniques have drawbacks. Northern blot is limited by its ... In comparison to the Northern blot, the reverse northern blot is able to probe a large number of transcripts at once with less ... Gene expression Northern blot RNA Seq Southern blot Primrose, Sandy B.; Twyman, Richard (2009-04-01). Principles of Genome ...
Similarly, detection of RNA is termed as northern blot. The term "western blot" was given by W. Neal Burnette in 1981, although ... Biology portal Technology portal Chemistry portal Far-eastern blot Far-western blot Eastern blot Northwestern blot Fast ... However, NC is brittle, and does not allow the blot to be used for re-probing, whereas PVDF membrane allows the blot to be re- ... The western blot (sometimes called the protein immunoblot), or western blotting, is a widely used analytical technique in ...
Southern blot for DNA northern blot for RNA reverse northern blot for RNA western blot for proteins far-western blot for ... The reverse northern blot differs from both northern and Southern blot in that DNA is first immobilized on a blotting matrix ... The northern blot is for the detection of specific RNA sequences in complex samples. Northern blotting first separates samples ... making the Far-Western blot a specific case of the Western blot. A southwestern blot is based on Southern blot and is used to ...
Southern blot Northern blot Western blot Southwestern blot "Test Blots, Slot Blots & Dot Blots - Immunodetection , Bio-Rad". ... Performing a dot blot is similar in idea to performing a western blot, with the advantage of faster speed and lower cost. Dot ... A dot blot (or slot blot) is a technique in molecular biology used to detect proteins. It represents a simplification of the ... A general dot blot protocol involves spotting 1-2 microliters of a samples onto a nitrocellulose or PVDF membrane and letting ...
"Northern Blotting". www.lifetechnologies.com. Retrieved 2016-11-26. Wang, X., Seed, B. (2003). A PCR primer bank for ... researchers have used different techniques such as the Northern Blot technique and Real-Time PCR. From these studies, it was ...
Bridged nucleic acid
Northern blotting; real-time PCR; in situ hybridization; functional analysis; SNP detection and use as antigens and many others ...
Patricia Thomas, developer of the RNA blot which then became known as the northern blot, actually didn't use the term. Named ... The northern blot is used to study the presence of specific RNA molecules as relative comparison among a set of different ... A western blot is a technique by which specific proteins can be detected from a mixture of proteins. Western blots can be used ... He SL, Green R (1 January 2013). "Northern blotting". Methods in Enzymology. 530: 75-87. doi:10.1016/B978-0-12-420037-1.00003-8 ...
Other blotting methods (i.e., western blot, northern blot, eastern blot, southwestern blot) that employ similar principles, but ... acids Restriction fragment Genetic fingerprint Northern blot Western blot Eastern blot Southwestern blot Northwestern blot " ... Southern blotting transfer may be used for homology-based cloning on the basis of amino acid sequence of the protein product of ... A Southern blot is a method used in molecular biology for detection of a specific DNA sequence in DNA samples. Southern ...
Glossary of genetics
Northern blotting nRNA See nuclear RNA. nuclear membrane A sub-cellular barrier consisting of two lipid bilayer membranes that ... Contents: Top 0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z western blotting whole genome sequencing (WGS) The ... Southern blot A molecular biology method used for detecting a specific sequence in DNA samples. The technique combines ...
Glossary of genetics (M−Z)
Northern blotting nRNA See nuclear RNA. nuclear membrane A sub-cellular barrier consisting of two lipid bilayer membranes that ... Contents Top A B C D E F G H I J K L M N O P Q R S T U V W X Y Z See also References External links western blotting whole ... Southern blotting A molecular biology method used for detecting a specific sequence in DNA samples. The technique combines ...
Southern blot Western blot Northern blot Southwestern blot Eastern blot Gel electrophoresis SDS-PAGE Chromatography ... These techniques include the western blot (protein detection), the northern blot (RNA detection), the southwestern blot (DNA- ... Northwestern Blot of Protein-RNA Interaction from Young Rice Panicles RNA Isolation and Northern Blot Analysis Protein Blotting ... is a hybrid analytical technique of the western blot and the northern blot, and is used in molecular biology to detect ...
x (2nd ed.). Viking Society for Northern Research. pp. 30-33. ISBN 978-0-903521-27-7. "A time for Blot". Reviving the Elder Way ... a-time-for-blot- Knowst How to Blot Definition of Blót - Zöega dict. Blót explained according to Heithni (CS1 German-language ... Hence it is not on 12 January in the current calendar.) Þorrablót (Iceland) Freyr blot (Sweden): The Óláfs saga Tryggvasonar en ... ISBN 3-533-04230-8. Näsström, Britt-Mari (Oslo 2001). Blot: Tro og offer i det førkristne Norden. ISBN 82-530-2146-1. Orel, ...
Aggregatibacter iron-regulated sRNA
The transcription of sRNAs was confirmed by Northern blot. Fur binding was demonstrated to each sRNA promoter, and that ...
Northern blot analysis detected 3 transcripts; a 1-kb transcript was predominant in heart, and a 3-kb transcript was ...
Northern blot analysis documented the samples. The suggested relation to metabolism and vascular disease was attributed to ...
Northern blot analysis also indicated this RNA undergoes specific cleavage processing. The GlmZ sRNA has been shown to ... The expression of SraJ was experimentally confirmed by Northern blotting. This ncRNA is expressed in early logarithmic phase, ...
Nuclease protection assay
Northern blotting is a laboratory technique that produces similar information. It is slower and less quantitative, but also ...
The stability and abundance of the shorter 3' transcript is confirmed in both Northern blotting and deep sequencing analysis. ... The expression of SraH was experimentally confirmed by Northern blotting. Its expression is highly abundant in stationary ...
Using Northern Blot Analysis, Vitelli et al. (1996) found that RAB7a was expressed as 1.7- and 2.5-kb transcripts in all cell ...
Teg49 small RNA
It was identified by RNA-seq and confirmed by Northern blot. It is modulated by sigB (sarA regulator) and cshA (an ATP- ...
Hfq binding sRNA
12 of them were confirmed by Northern blot. DicF RNA DsrA RNA FnrS RNA GadY RNA GcvB RNA IsrJ RNA MicA RNA / SraD RNA MicC RNA ...
Karen Lehrman Bloch
"In this age of leftist illiberalism, I'm standing up for the truth". The Jewish News of Northern California. May 7, 2021. ... Pollitt, Katha (June 23, 1997). "'Lipstick Blots' on The Feminist Debate". The Washington Post. Suqi, Rima (September 25, 2013 ...
The expression of novel ncRNA of interest was confirmed by northern blotting. In this large scale screen these ncRNAs were ...
From this study multiple mRNA transcripts were detected by northern blot analysis. This finding suggests that netrin receptors ...
Small nucleolar RNA SNORD45
U45 was originally cloned from HeLa cells and expression verified by northern blotting. It is related to the snoRNA (MBII-401) ...
Small nucleolar RNA SNORD44
U44 was originally cloned from HeLa cells and expression verified by northern blotting. It is predicted to guide 2'O-ribose ...
Expression of 11 of them was confirmed by Northern blot. The sRNAs were shown to be only present in Streptomyces species. sRNA ...
A notable example occurs at Fyrkat, in the northern Jutland region of Denmark. Fyrkat is the site of a former Viking Age ring ... Näsström, B.M. (2002). Blot, tro och offer i det förkristna Norden. Norstedts. ISBN 91-7297-033-2. Näsström, B.M. (2009). ... The Albis rises in the Vandalic Mountains, and empties, a mighty river, into the northern ocean. Drusus undertook to cross this ... ISBN 0-415-12034-9. Ellis Davidson, H.R. (1964). Gods and Myths of Northern Europe. Penguin Books. Enright, M.J. (1996). Lady ...
Small nucleolar RNA SNORD47
This snoRNA was originally cloned from HeLa cells and expression verified by northern blotting. It is predicted to guide 2'O- ...
It may also land on a point occupied by exactly one opposing checker, or "blot". In this case, the blot has been "hit" and is ... Northern Cyprus. When backgammon is played for money, the most common arrangement is to assign a monetary value to each point, ... A blot has the highest probability of being hit when it is 6 points away from an opponent's checker. Strategies can derive from ... For example, players may position all of their blots in such a way that the opponent must roll a 2 in order to hit any of them ...
7th Arkansas Infantry Regiment
... bl&ots=7ptDBF0n2D&sig=-K_6PQoHglmh_SOzuobv_JyNWUw&hl=en#v=onepage&q=Confederate%20conscription%20law%20reorganization% ... northern Mississippi, and Alabama to reorganize. Shaver's brigade guarded the Confederate rear during this retreat from Bowling ... Lee surrendered the Army of Northern Virginia. The regiment was surrendered with the rest of the Army of Tennessee on April 26 ...
Battle of Kousséri
In response, a French column proceeding from Gabon and led by Émile Gentil, supported by the steamboat Leon Blot, confronted ... in what today is northern Cameroon. In 1899, Sudanese warlord Rabih az-Zubayr could field some 10,000 infantry and cavalry, all ...
ELISA screening and Western blot confirming); or 3) single-tier IgG (old infection) Western blot. Previously, the CDC only ... In northern Africa, B. burgdorferi sensu lato has been identified in Morocco, Algeria, Egypt and Tunisia. Lyme disease in sub- ... Tests for antibodies in the blood by ELISA and Western blot is the most widely used method for Lyme diagnosis. A two-tiered ... Lyme disease is the most common disease spread by ticks in the Northern Hemisphere. An estimated 476,000 people a year are ...
Roger B. Taney
However, after two of the Northern justices objected to the decision, Taney and his four Southern colleagues decided to write a ... Taney delivered "an impassioned defense of Gruber" and, in his opening argument, Taney condemned slavery as "a blot on our ... Like the other Southerners on the Court, Taney was outraged over what he saw as "Northern aggression" towards slavery, an ... At the request of Associate Justice John Catron, Buchanan convinced Northern Associate Justice Robert Cooper Grier to join the ...
The band toured Northern Europe and Canada throughout the 1980s and 1990s. With their Nordic pagan approach, the band had a big ... 1992 Mid winter Blot, Sentrum Scene, Oslo, Norway July 24, 1993 Nordic House in the Faroe Islands June 11, 1994 TMV Festival, ...
Anti small RNA
Recent experiments have used Northern blot analysis and 5'-end mapping to correctly identify potential antisense sRNA ...
Writers such as Charles-Edwards, Waldman and Mason had suggested a Coriondi link with a Northern Celtic tribe of Ancient ... bl&ots=YLtqK9I_RF&sig=ACfU3U3g8uC10IP7IQ6ybXArz3QspwQWVQ&hl=en&sa=X&ved=2ahUKEwj6ntyrkMXxAhVSQMAKHd-gAbgQ6AEwBXoECBYQAw#v= ... more tan likely related to the Northern Uí Néill. The Kingdom of Gwynedd was founded by the Venicones who were part of the ... Túathal Techtmar was a leader of the northern branch of the Venii(tribes-men) in Ireland and notably lead the overthrow of the ...
Pseudomonas sRNA P16
... tools in the genome of the opportunistic pathogen Pseudomonas aeruginosa and its expression verified by northern blot analysis ...
Northern Italian influence is assumed also for golden plaques with Christian motifs from Bojná (probably from a portable altar ... have blotted out, but over which in former days [Svatopluk] used to rule. Such are the landmarks and names along the Danube ... The fate of the northern and western parts of former Central Europe in the 10th century is thus largely unclear. The eastern ... The earliest possible reference to Slavic tribes living in the valley of the northern Morava river was made by the Byzantine ...
On July 17, 1896, Crown Prince Mohammad Ali Mirza beheaded them in the northern garden of Tabriz on charges of being Bábist and ... bl&ots=iiA8KSlJBa&sig=ACfU3U3P8h42KQn_nMREtDJZ3p3UQSYohQ&hl=en&sa=X&ved=2ahUKEwigv9q-5fToAhWEYsAKHfSNBxAQ6AEwAHoECAIQKA#v= ... Available through: https://books.google.com.pk/books?id=L-T-ng2v47QC&pg=PA85&lpg=PA85&dq=Maryam+Amid&source=bl&ots=Wr8cqnmByp& ...
Timeline of United States inventions (1890-1945)
However, paper towels can only be used once after they blot wet surfaces. A school teacher in Ashland, Ohio, named Kurt Klier, ... Toffee apples are a common treat at autumn festivals in Western culture in the Northern Hemisphere, such as Halloween and Guy ...
The failure of his campaign to check Calvin Coolidge's clean sweep of the northern industrial states, persuaded Beal that "the ... "completely blotted out" his record as a strike leader and as a victim of the "Gastonia frame up". But the Hearst papers, he ...
Climate of Dubai
"Major sandstorm blots out Dubai sky". BBC News. 2 April 2015. Retrieved 24 June 2018. "UAE weather: Rain and 60kph high winds ... "Flood warning for Northern Emirates after thick dust blankets Abu Dhabi and Dubai". thenationalnews.com. 14 August 2022. ...
The size of the invasion force (no more than 7000 troops in the northern theater) was simply too small to storm or lay siege to ... The only blot was that his first wife Tala Mi Daw, who had just delivered their first (and only) son, refused to join him in ... Ava retained a toehold at the Khway-thin-taung garrison in northern Arakan until 1416/17 but it did not send a force to retake ... But the expected invasion never came as Ava was forced to deal with breaches into its northern borders by the powerful Shan ...
Although three transcripts are detected in northern blots, there is currently no sequence available to confirm transcript ...
Reverse transcription polymerase chain reaction
Northern blot analysis is used to study the RNA's gene expression further. RT-PCR can also be very useful in the insertion of ... However, since PCR was invented by Kary Mullis in 1983, RT PCR has since displaced Northern blot as the method of choice for ... Streit S, Michalski CW, Erkan M, Kleeff J, Friess H (2009). "Northern blot analysis for detection and quantification of RNA in ... Northern blot has been used extensively for RNA quantification despite its shortcomings: (a) time-consuming technique, (b) ...
Independent Commission Against Corruption (New South Wales)
Loane, Sally (29 April 1994). "ICAC Laws blot on NSW". The Sydney Morning Herald. Retrieved 18 April 2014. Moore, Matthew; ... Northern Territory) Independent Commission Against Corruption (South Australia) Royal Commission into the New South Wales ...
... followed by confirmation using Western Blot test. It cannot be cultured from synovial fluid. However, PCR testing yields 85% ... "A Retrospective Case-Series of Children With Bone and Joint Infection From Northern Australia". Medicine. 95 (8): e2885. doi: ...
In men whom men pronounce divine I find so much of sin and blot I do not dare to draw a line Between the two, where God has not ... Spending a short time in the mining camps of northern Idaho, Miller found his way to Canyon City, Oregon by 1864 where he was ... As a young man, he moved to northern California during the California Gold Rush years, and had a variety of adventures, ...
The highest-known juniper forest occurs at an altitude of 4,900 metres (16,100 ft) in southeastern Tibet and the northern ... bl&ots=SkBfZip0TX&sig=ACfU3U3FQip4K8a3YUN_xmDfTa45U4d-Ig&hl=en&sa=X&ved=2ahUKEwjt4Zj9zp_7AhV7BzQIHQRwBkgQ6AF6BAgwEAM#v=onepage& ... Depending on the taxonomy, between 50 and 67 species of junipers are widely distributed throughout the Northern Hemisphere, ... Juniper in weave is a traditional cladding technique used in Northern Europe, e.g. at Havrå, Norway. Juniper berries are steam ...
... bl&ots=ECBWeOiDgE&sig=rPuoQ_eipSBBwxmznojOxuyCvx8&hl=en&ei=gk99Tb7kKI_6swPv6_yIAw&sa=X&oi=book_result&ct=result&resnum=1&sqi=2& ... The Great Sioux War of 1876-1877 was a series of conflicts involving the Lakota and Northern Cheyenne tribes. Following the ... bl&ots=COJBQlr1cJ&sig=zVBwjjJqG7_NmkGV22a4RGVy-ow&hl=en&ei=LlB9TeK8CY_urAGn1dHWBQ&sa=X&oi=book_result&ct=result&resnum=1&ved= ...
... mRNA as assayed by northern blot analysis. TGFβ1 also increased PTPkappa mRNA and protein in normal and tumor mammary cell ...
... bl&ots=YmeF_6v6Yx&sig=yWtI6yD6ow-qcuXNe_8zksjFU7M&hl=en&sa=X&ei=OiLeVJ3JJo6X7QaXloHIDg&ved=0CCoQ6AEwATgo#v=onepage&q=Guillem% ... Northern Ireland 2007, 2008: London: Bernard Chauchet Gallery, London SW10. (June and December collectives). 2008: London: ...
Dual Sarcocystis neurona and Toxoplasma gondii infection in a Northern sea otter from Washington state, USA. Vet Parasitol. ... also called the Western Blot (WB) test. Development of the WB test over the years has benefited greatly in EPM diagnosis, ...
Wilde spent mid-1897 with Robert Ross in the seaside village of Berneval-le-Grand in northern France, where he wrote The Ballad ... "the only blot on my tutorship". The University Philosophical Society also provided an education, as members discussed ...
... as a blot on the landscape. As a result, it is difficult to get a decent view of the white windmills of the windfarm at the ... ". "A Musical Bridge". Northern Constitution. 1 August 1908. "Witness Statement: Patrick Hegarty" (PDF). Bureau of Military ...
Members - Analitycal, bioanalitycal methods - Northern & Southern Blot
Northern Blot probes (non-radioactive)
Applications of northern blotting? - Lifeeasy Biology: Questions and Answers
BIO TECH KIT - Northern Blotting Teaching Kit Manufacturer from Chennai
Northern Blotting Teaching Kit, Total RNA Extraction from Bacteria Teaching Kit, Total RNA Extraction and RT-PCR Teaching Kit ... Northern blotting takes its name from its similarity to the first blotting technique, the Southern blot, named for biologist ... However, the entire process is commonly referred to as northern blotting. The northern blot technique was developed in 1977 by ... northern blot actually refers specifically to the capillary transfer of RNA from the electrophoresis gel to the blotting ...
The Perennial Northern Blot of Lopez-Otin - For Better Science
Cancer researcher Carlos López-Otín published the same Northern blot no less than 23 times in 23 publications, between 1994 and ... The Perennial Northern Blot of Lopez-Otin. Cancer researcher Carlos López-Otín published the same Northern blot no less than 23 ... A Perennial Northern Blot, by Smut Clyde. The title of this post refers to the famously picaresque Western blot belonging to a ... pioneer of Southern blotting, for this is how humor works in molecular biology. It has been explained to me that Northern blots ...
RNA (环RNA) 的非同位素标记Northern blot -BIO-101
Gene Expression Literature Detail
RNA: A Laboratory Manual
10 Northern Blots: Alternative Method for Processing Northern Blots after Capillary Transfer. 11 Northern Blots for Small RNAs ... 8 Northern Blots: Denaturation and Electrophoresis of RNA with Glyoxal. 9 Northern Blots: Capillary Transfer of RNA from ... Tails Using Northern Blotting Method 2: High-resolution Measurement of Poly(A) Tails Using Northern Blotting Method 3: PCR- ... Northern Blotting. Nuclease Protection. Primer Extension. Other Methods. PCR-based Approaches. Specialized Methods for ...
Noncanonical CTD kinases regulate RNA polymerase II in a gene-class-specific manner | Nature Chemical Biology
Asymptomatic Visceral Leishmaniasis, Northern Israel - Volume 9, Number 3-March 2003 - Emerging Infectious Diseases journal -...
Western blotting was used to confirm asymptomatic VL (8). Strong reactions were observed to 14- and/or 18-kDa antigens with all ... Asymptomatic Visceral Leishmaniasis, Northern Israel. Volume 9, Number 3-March 2003. Article Views: 202. Data is collected ... HVL is endemic in northern Israel; 68 cases, mostly from Arab villages, were documented from 1960 to 1989 (5). Since 1993, a ... Asymptomatic Visceral Leishmaniasis, Northern Israel. Emerging Infectious Diseases. 2003;9(3):397-398. doi:10.3201/ ...
Expression of the two mannose 6-phosphate receptors is spatially and temporally different during mouse embryogenesis |...
Northern blot analysis showing spccifity of the MPR46 and MPR300 probes. Total and poly(A)+ RNA, 15 and 1.5 μg respectively, ... Northern blot analysis showing spccifity of the MPR46 and MPR300 probes. Total and poly(A)+ RNA, 15 and 1.5 μg respectively, ... For Northern blot analysis (Maniatis et al., 1982) RNA was labelled with [32P]UTP, for in situ hybridization with [35S]UTP ... Northern blot analysis of MPR46 and MPR300 expression. The probes for the MPR46 and MPR300 hybridize to specific RNA species of ...
Northern blot analysis demonstrated that relative to controls, the size of the IP3R1 transcript in opt mice was reduced. ... Expression of IP3R1 mRNA in the brains ofopt mice was examined by Northern blot analysis.Miwh homozygote mice displayed a ... This blot was probed with EF 1 α (Xiang and Werner, 1989) to control for lane loading. B, RT-PCR analysis of IP3R1 mRNA with ... C, Southern blot hybridization analysis demonstrates that a 3.4 kb NsiI fragment is deleted in opt genomic DNA. D, The intron/ ...
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C. Brenner: Explanation of the Lyme Disease Western Blot
The Western blot, which identifies specific antibody proteins, is but one kind of immunoblot; *there is also a Northern blot, ... and a Southern blot, which does the same for DNA sequences. In a Western blot, the testing laboratory looks for antibodies ... Explanation of the Lyme Disease Western Blot. by Carl Brenner. Inquiries about various issues relating to Western blot (WB) ... A second criticism of the CDC Western blot criteria is that they fail to include the 31 and 34 kDa bands. This does indeed seem ...
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Such terms as Southern (transfer of DNA), Northern (transfer of RNA), and Western (transfer of protein) blots have been ... Nitrocellulose paper used in Western blot test--fire and potential explosion hazard. MMWR 1985;34:426.. POINT OF CONTACT FOR ... NC sheets up to 20 x 20 cm are often used in these blotting procedures.. Additionally, large circles (up to 138 mm diameter) ... blotted) from the gel onto the NC. ...
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This wreaked havoc on agriculture, leading to famines all across the Northern hemisphere. It was the year without summer- one ... blotting out the sun for almost an entire year. ... blotting out the sun for almost an entire year. This wreaked ... havoc on agriculture, leading to famines all across the Northern hemisphere. It was the year without summer- one of the darkest ...
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Additional file 2: Quantification of GCN4 mRNA by northern blot hybridization. (A) Northern blot hybridization of GCN4 mRNA. We ... B) Quantification of northern blot hybridization signals. Chemiluminescent signals of the standard RNA in (A) were quantified ... Hybridization-based methods include northern blot hybridization and those based on liquid-phase hybridization, such as nuclease ... These results were consistent with those obtained by northern blot hybridization (Additional data file 2) and real-time PCR ( ...
- The northern blot technique was developed in 1977 by James Alwine, David Kemp, and George Stark at Stanford University. (orangeindia.co.in)
- Northern blotting takes its name from its similarity to the first blotting technique, the Southern blot, named for biologist Edwin Southern. (orangeindia.co.in)
- Northern blotting is a widely used technique to study sRNAs because it is quantitative, relatively inexpensive, and readily available for most laboratories. (bvsalud.org)
- First of all, it should be noted that the Western blot is usually performed as a follow-up to an ELISA test, which is the most commonly employed initial test for Lyme disease. (lymenet.de)
- for example, the usual testing regime for HIV is also an initial ELISA followed by a confirmatory Western blot. (lymenet.de)
- Both the ELISA and the Western blot are "indirect" tests -- that is, they measure the immune system's response to an infectious agent rather than looking for components of the agent itself. (lymenet.de)
- ABSTRACT Crude antigenic preparations from Setaria equina were used in ELISA and Western blotting to examine cross- reaction with human sera from areas endemic for bancroftian filariasis. (who.int)
- ELISA et transfert Western afin d'étudier la réaction croisée avec des sérums humains en provenance de zones endémiques pour la filariose de Bancroft. (who.int)
- A family of DNA fragments is generated and this can Northern Blotting be visualized on a Polyacrylamide gel. (who.int)
- This chapter describes the protocols for radioactive and non-radioactive sRNA Northern blot analysis, which includes RNA extraction, polyacrylamide gel electrophoresis, membrane transfer, hybridisation and detection of sRNA using oligonucleotide probes. (bvsalud.org)
- The second part of the chapter includes a method used to analyze bacterial gene expression (Northern blotting), two methods to generate radioactive probes, as well as target detection using a phosphorimager. (bvsalud.org)
- Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES. (bvsalud.org)
- Eventually, an image of a Northern blot (showing expression of mRNAs which code for proteins) was found to appear recurrently across several papers from that Oviedo lab, where the authors pretended it was a newly produced analysis. (forbetterscience.com)
- Commonly, nucleic acids or proteins are electrophoretically separated on agarose or acrylamide gels and then transferred (blotted) from the gel onto the NC. (cdc.gov)
- In a Western blot, the testing laboratory looks for antibodies directed against a wide range of Bb proteins. (lymenet.de)
- This is done by first disrupting Bb cells with an electrical current and then "blotting" the separated proteins onto a paper or nylon sheet. (lymenet.de)
- However, northern blot analyses of guts of bees fed extracts of honey, pollen and propolis showed elevated expression of three CYP6AS P450 genes. (bijensterfte.nl)
- The ability to obtain clean and undegraded RNA could be challenging and daunting and remains the most crucial experimental step prior to downstream analyses, such as Northern blotting, quantitative PCR (qPCR), and RNA-seq.This chapter describe two methods (acid guanidinium thiocyanate (TRIzol) phenol-chloroform and hot phenol) commonly used to isolate total bacterial RNA and are suitable for both Gram-positive and Gram-negative bacteria. (bvsalud.org)
- Northern blot analysis is useful for revealing the size of the mRNA encoded by a gene. (lifeeasy.org)
- Northern blot analysis can be used to investigate whether an mRNA is present in a cell type or tissue and in how much quantity it is present. (lifeeasy.org)
- RNA was extracted from some cells and the extent of expression of inducible NOS mRNA was determined by Northern blotting. (cdc.gov)
- Messenger RNA (mRNA) levels of c- myc, a gene regulated by NF-kappa-B, were determined via Northern blot analysis. (cdc.gov)
- The term 'northern blot' actually refers specifically to the capillary transfer of RNA from the electrophoresis gel to the blotting membrane. (orangeindia.co.in)
- After deionization and lyophilization, two-dimensional gel electrophoresis was used for protein separation followed by Western blotting. (who.int)
- Western blotting was used to confirm asymptomatic VL ( 8 ). (cdc.gov)
- Such terms as 'Southern' (transfer of DNA), 'Northern' (transfer of RNA), and 'Western' (transfer of protein) blots have been coined. (cdc.gov)
- The following paper written by Carl Brenner is a wonderful reference for those trying to better understand the Lyme disease Western blot test. (lymenet.de)
- Inquiries about various issues relating to Western blot (WB) testing are frequently posted to the Lyme disease discussion groups on the Internet. (lymenet.de)
- The following article is offered as a primer in the techniques and interpretation of Western blotting, and should help most patients navigate their way through some of the medical and scientific terminology associated with the assay. (lymenet.de)
- Immune complexes present on the blot are then detected using a labeled second antibody. (who.int)
- However, the entire process is commonly referred to as northern blotting. (orangeindia.co.in)
- Hybridization-based methods include northern blot hybridization and those based on liquid-phase hybridization, such as nuclease protection assay. (biomedcentral.com)
- Northern blot, microarray, and RT-qPCR) and emerging (e.g., nanostructured materials and electrochemical methods) techniques have been developed to detect miRNA, each with their own strengths and weaknesses. (bvsalud.org)
- Any specific DNA present on the blot will combine labeled DNA to neutral dimethylsulphate acid, with the labeled probe and is detected auto hydrazine NaCl piperidine or hydrazine piperidine. (who.int)
- In this study, HVL seroprevalence in northern Israel was compared with that in a region free of clinical disease. (cdc.gov)
- HVL has not been reported) and 11 sites (2,086 serum samples) in the HVL-endemic northern region, where the disease was previously reported in 8 of 11 sites ( Figure ). (cdc.gov)
- says Raymond Blot, a resident of Elbeuf, in the northern Normandy region. (ksut.org)
- In 1815, Mount Tambora erupted and its emissions spread across the globe, blotting out the sun for almost an entire year. (ted.com)
- Lopez-Otin's data integrity issues involve also such obviously manipulated gels like this Northern Blot in Llano et al Cancer Research 1999 . (forbetterscience.com)
- Northern blotting was used to determine the approximate size, abundance and any processing events attributed to the sRNAs. (bvsalud.org)
- Notable among these were a study of hard-metal workers in several small factories in northern Italy. (who.int)
- essential so that reemergence of residual tissue or a new disease can be detected hence treatment Southern Blotting intervention can be instituted. (who.int)
- Incidentally, also Lopez-Otin's Perennial Northern Blot made its first appearance at around that year. (forbetterscience.com)
- Protein expression will be analyzed by immunohistochemistry (for localization) and western blotting (for quantification). (nih.gov)
- A variety of techniques-including in situ hybridization, immunohistochemistry, RT-PCR, Western and Northern blotting, and immunoprecipitation-are used to analyze gene and protein expression, function, and interactions. (nih.gov)
- Western blotting was used to confirm asymptomatic VL ( 8 ). (cdc.gov)
- About 10 years ago, the Maraia Lab developed an assay, based on a northern blot, to identify these types of mutations in tRNA modification enzymes (TME). (nih.gov)
- Northern-blot analysis of poly(A)+ RNA from several human tissues indicates a complex expression pattern differing across tissues. (tcdb.org)
- In some experiments, changes in NEP-specific mRNAs in the presence or absence of dexamethasone and/or the inhibitors were also evaluated by Northern blot analysis. (cdc.gov)
- Northern blot analysis indicated that NEP-specific mRNAs were also increased by dexamethasone. (cdc.gov)
- Messenger RNA (mRNA) levels of c- myc, a gene regulated by NF-kappa-B, were determined via Northern blot analysis. (cdc.gov)
- 19. [mRNA expression of metastasis-suppressor gene nm23 in carcinoma of buccal mucosa I. Northern blotting study]. (nih.gov)
- Our laboratory developed a northern blot method that can quantify relative levels of base modifications on multiple specific tRNAs ∼10 yr ago, which has been used to characterize four different TME deficiencies and is likely further extendable. (nih.gov)
- Falsified the Northern blots in multiple panels of Figure 3, MCB 20:4428-4435, 2000. (nih.gov)