Direct association of Bloom's syndrome gene product with the human mismatch repair protein MLH1. (1/66)

Bloom's syndrome (BS) is a rare genetic disorder characterised by genomic instability and cancer susceptibility. BLM, the gene mutated in BS, encodes a member of the RecQ family of DNA helicases. Here, we identify hMLH1, which is involved in mismatch repair (MMR) and recombination, as a protein that directly interacts with BLM both in vivo and in vitro, and that the two proteins co-localise to discrete nuclear foci. The interaction between BLM and hMLH1 appears to have been evolutionarily conserved, as Sgs1p, the Saccharomyces cerevisiae homologue of BLM, interacts with yeast Mlh1p. However, cell extracts derived from BS patients show no obvious defects in MMR compared to wild-type- and BLM-complemented BS cell extracts. We conclude that the hMLH1-BLM interaction is not essential for post-replicative MMR, but, more likely, is required for some aspect of genetic recombination.  (+info)

Mutational analysis of the regulatory function of the c-Abl Src homology 3 domain. (2/66)

The catalytic activity of the c-Abl tyrosine kinase is tightly regulated by its Src homology 3 (SH3) domain through a complex mechanism that may involve intramolecular binding to Pro242 in the linker region between the SH2 and catalytic domains as well as interactions with a trans-inhibitor. We analysed the effect of mutation or replacement of SH3 on c-Abl tyrosine kinase activity and transformation. Random mutagenesis of SH3 identified several novel point mutations that dysregulated c-Abl kinase activity in vivo, but the RT loop was insensitive to mutational activation. Activating SH3 mutations abolished binding of proline-rich SH3 ligands in vitro, while mutations at Ser140 in the connector between the SH3 and SH2 domains activated Abl kinase activity in vivo and in vitro but did not impair SH3 ligand-binding. Abl was regulated efficiently when its SH3 domain was replaced with a heterologous SH3 from c-Src that binds a different spectrum of proline-rich ligands, but not by substitution of a modular WW domain with similar ligand-binding specificity. These results suggest that the SH3 domain regulates Abl principally by binding to the atypical intramolecular ligand Pro242 rather than a canonical PxxP ligand. Coordination between the SH3 and SH2 domains mediated by the connector region may be required for regulation of Abl even in the absence of SH2 ligand binding.  (+info)

The small 11-kDa protein from B19 parvovirus binds growth factor receptor-binding protein 2 in vitro in a Src homology 3 domain/ligand-dependent manner. (3/66)

The small 11-kDa proteins of B19 parvovirus contain three proline-rich regions which conform to consensus Src homology 3 (SH3) ligand sequences present in signaling molecules within the cell. We have shown that the B19 11-kDa proteins specifically interact with the growth factor receptor-binding protein 2 (Grb2) in vitro. Mutation of prolines within one of the three SH3 ligand-like sequences decreases the binding of B19 11-kDa proteins to Grb2, suggesting that the proline-rich region is involved in the B19 11-kDa/Grb2 interaction. Therefore, the B19 11-kDa proteins may function to alter Grb2-mediated signaling by disrupting SH3 domain/ligand interactions. These results implicate the 11-kDa proteins in B19 pathogenesis through perturbation of normal cellular signaling pathways.  (+info)

In vivo gene transfer of endothelial nitric oxide synthase decreases portal pressure in anaesthetised carbon tetrachloride cirrhotic rats. (4/66)

BACKGROUND: Portal hypertension in cirrhosis results from enhanced intrahepatic resistance to an augmented inflow. The former is partly due to an imbalance between intrahepatic vasoconstriction and vasodilatation. Enhanced endothelin-1 and decreased activity of hepatic constitutive endothelial nitric oxide synthase (NOS 3) was reported in carbon tetrachloride (CCl(4)) cirrhotic rat liver. AIMS: To study whether an increase in hepatic NOS 3 could be obtained in the CCl(4) cirrhotic rat liver by in vivo cDNA transfer and to investigate a possible effect on portal pressure. METHODS: Hepatic NOS 3 immunohistochemistry and western blotting were used to measure the amount of NOS 3 protein. Recombinant adenovirus, carrying cDNA encoding human NOS 3, was injected into the portal vein of CCl(4) cirrhotic rats. Cirrhotic controls received carrier buffer, naked adenovirus, or adenovirus carrying the lac Z gene. RESULTS: NOS 3 immunoreactivity and amount of protein (western blotting) were significantly decreased in CCl(4) cirrhotic livers. Following cDNA transfer, NOS 3 expression and the amount of protein were partially restored. Portal pressure was 11.4 (1.6) mm Hg in untreated cirrhotic (n=9) and 11.8 (0.6) in lac Z transfected (n=4) cirrhotic rats but was reduced to 7.8 (1.0) mm Hg (n=9) five days after NOS 3 cDNA transfer. No changes were observed in systemic haemodynamics, in liver tests or urinary nitrates, or in NOS 3 expression in lung or kidney, indicating a highly selective transfer. CONCLUSIONS: NOS 3 cDNA transfer to cirrhotic rat liver is feasible and the increase in hepatic NOS 3 leads to a marked decrease in portal hypertension without systemic effects. These data indicate a major haemodynamic role of intrahepatic NOS 3 in the pathogenesis of portal hypertension in CCl(4) cirrhosis.  (+info)

A chromatin-associated protein from pea seeds preferentially binds histones H3 and H4. (5/66)

Pisum sativum p16 is a protein present in the chromatin of ungerminated embryonic axes. The purification of p16 and the isolation of a cDNA clone of psp54, the gene encoding its precursor have been recently reported [Castillo, J., Rodrigo, M. I., Marquez, J. A., Zuniga, A and Franco, L. (2000) Eur. J. Biochem.267, 2156-2165]. In the present paper, we present data showing that p16 is a nuclear protein. First, after subcellular fractionation, p16 was clearly found in nuclei, although the protein is also present in other organelles. Immunocytochemical methods also confirm the above results. p16 seems to be firmly anchored to chromatin, as only extensive DNase I digestion of nuclei allows its release. Far Western and pull-down experiments demonstrate a strong in vitro interaction between p16 and histones, especially H3 and H4, suggesting that p16 is tethered to chromatin through histones. Because the psp54 gene is specifically expressed during the late development of seed, the role of p16 might be related to the changes that occur in chromatin during the processes of seed maturation and germination.  (+info)

Profiling the global tyrosine phosphorylation state. (6/66)

Protein tyrosine kinases and protein tyrosine phosphatases play a key role in cell signaling, and the recent success of specific tyrosine kinase inhibitors in cancer treatment strongly validates the clinical relevance of basic research on tyrosine phosphorylation. Functional profiling of the tyrosine phosphoproteome is likely to lead to the identification of novel targets for drug discovery and provide a basis for novel molecular diagnostic approaches. The ultimate aim of current mass spectrometry-based phosphoproteomic approaches is the comprehensive characterization of the phosphoproteome. However, current methods are not yet sensitive enough for routine detection of a large percentage of tyrosine-phosphorylated proteins, which are generally of low abundance. In this article, we discuss alternative methods that exploit Src homology 2 (SH2) domains for profiling the tyrosine phosphoproteome. SH2 domains are small protein modules that bind specifically to tyrosine-phosphorylated peptides; there are more than 100 SH2 domains in the human genome, and different SH2 domains bind to different classes of tyrosine-phosphorylated ligands. These domains play a critical role in the propagation of signals in the cell, mediating the relocalization and complex formation of proteins in response to changes in tyrosine phosphorylation. We have developed an SH2 profiling method based on far-Western blotting, in which a battery of SH2 domains is used to probe the global state of tyrosine phosphorylation. Application to the classification of human malignancies suggests that this approach has potential as a molecular diagnostic tool. We also describe ongoing efforts to modify and improve SH2 profiling, including the development of a multiplexed assay system that will allow high-throughput functional profiling of the tyrosine phosphoproteome.  (+info)

Identification and characterization of a novel calcineurin-binding protein in scallop testis. (7/66)

Calcineurin has been inferred to function in meiosis and spermiogenesis in testis. Here, we identified a calcineurin-binding protein in scallop testis by Far-Western blot analysis using purified calcineurin as a probe. The molecular mass of the binding protein estimated on the blot was 75 kDa. The isolated cDNA clone encoded a novel 474-residue protein, named CaNBP75. The region between T6 and A210 of CaNBP75 was responsible for the interaction with calcineurin. CaNBP75 was predominantly expressed in testis and ovary of scallop. Thus, CaNBP75 may modulate the physiological function of calcineurin in the testis and ovary of scallop, such as in spermiogenesis or meiosis.  (+info)

Eukaryotic translation-initiation factor eIF2beta binds to protein kinase CK2: effects on CK2alpha activity. (8/66)

eIF2 (eukaryotic translation-initiation factor 2) is a substrate and an interacting partner for CK2 (protein kinase CK2). Co-immuno-precipitation of CK2 with eIF2beta has now been observed in HeLa cells, overexpressing haemagglutinin-tagged human recombinant eIF2beta. A direct association between His6-tagged human recombinant forms of eIF2beta subunit and both the catalytic (CK2alpha) and the regulatory (CK2beta) subunits of CK2 has also been shown by using different techniques. Surface plasmon resonance analysis indicated a high affinity in the interaction between eIF2beta and CK2alpha, whereas the affinity for the association with CK2beta is much lower. Free CK2alpha is unable to phosphorylate eIF2beta, whereas up to 1.2 mol of phosphate/mol of eIF2beta was incorporated by the reconstituted CK2 holoenzyme. The N-terminal third part of eIF2beta is dispensable for binding to either CK2alpha or CK2beta, although it contains the phosphorylation sites for CK2. The remaining central/C-terminal part of eIF2beta is not phosphorylated by CK2, but is sufficient for binding to both CK2 subunits. The presence of eIF2beta inhibited CK2alpha activity on calmodulin and beta-casein, but it had a minor effect on that of the reconstituted CK2 holoenzyme. The truncated forms corresponding to the N-terminal or central/C-terminal regions of eIF2beta were much less inhibitory than the intact subunit. The results demonstrate that the ability to associate with CK2 subunits and to serve as a CK2 substrate are confined to different regions in eIF2beta and that it may act as an inhibitor on CK2alpha.  (+info)

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"Western Blotting Inaccuracies with Unverified Antibodies: Need for a Western Blotting Minimal Reporting Standard (WBMRS)". PLOS ... Normalization of Western blot data is an analytical step that is performed to compare the relative abundance of a specific ... 2015-01-01). "Western Blotting Using In-Gel Protein Labeling as a Normalization Control: Stain-Free Technology". In Posch, ... When using fluorescent antibodies to image proteins in western blots, normalization requires that the user define the upper and ...
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Western blot Northwestern blot Far-eastern blot Blot Freeze, HH (1993). "Preparation and analysis of glycoconjugates". Current ... Since this is essentially a western blot, the charge reversal was used to dub this method an eastern blot. (2005) Eastern blot ... distinguishing them from a standard far-western blot. In principle, eastern blotting is similar to lectin blotting (i.e., ... Thus, eastern blot can be considered an extension of the biochemical technique of western blot. Multiple techniques have been ...
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Kurien BT, Scofield RH (April 2006). "Western blotting". Methods. 38 (4): 283-93. doi:10.1016/j.ymeth.2005.11.007. PMID ... in Western blot analyses to identify proteins separated by electrophoresis, and in immunohistochemistry or immunofluorescence ... Western blot, immunodiffusion, immunoelectrophoresis, and magnetic immunoassay. Antibodies raised against human chorionic ... antibody Optimer Ligand Secondary antibodies Single-domain antibody Slope spectroscopy Synthetic antibody Western blot ...
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A western blot is a technique by which specific proteins can be detected from a mixture of proteins. Western blots can be used ... "Western blot , Learn Science at Scitable". www.nature.com. Retrieved 2021-11-25. Kurien BT, Scofield RH (April 2006). "Western ... Using western blotting techniques allows not only detection but also quantitative analysis. Analogous methods to western ... In western blotting, proteins are first separated by size, in a thin gel sandwiched between two glass plates in a technique ...
Other blotting methods (i.e., western blot, northern blot, eastern blot, southwestern blot) that employ similar principles, but ... acids Restriction fragment Genetic fingerprint Northern blot Western blot Eastern blot Southwestern blot Northwestern blot " ... Burnette, W. Neal (April 1981). "Western Blotting: Electrophoretic Transfer of Proteins from Sodium Dodecyl Sulfate- ... The names for other blotting methods may follow this convention, by analogy. Southern invented Southern blot after combining ...
and Western blotting Löf, Liza; Arngården, Linda; Ebai, Tonge; Landegren, Ulf; Söderberg, Ola; Kamali-Moghaddam, Masood (2017 ... November 2011). "Western blotting via proximity ligation for high performance protein analysis". Molecular & Cellular ...
Western blotting is a process by which proteins separated in the acrylamide gel are electrophoretically transferred to a stable ... Western blot). After staining, different species biomolecules appear as distinct bands within the gel. It is common to run ... Agarose gel electrophoresis Capillary electrophoresis DNA electrophoresis Eastern blotting Electroblotting Fast parallel ... focusing Isotachophoresis Native gel electrophoresis Northern blotting Protein electrophoresis QPNC-PAGE Southern blotting Two ...
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Development of Western blotting technique to detect proteins. Publication of two protocols for plant transgenesis, which were ...
northern blotting A blotting method in molecular biology used to detect RNA in a sample. Compare Southern blotting, western ... Top A B C D E F G H I J K L M N O P Q R S T U V W X Y Z See also References External links Warburg effect western blotting ... Southern blotting A molecular biology method used for detecting a specific sequence in DNA samples. The technique combines ... blotting, and eastern blotting. nRNA See nuclear RNA. nuclear cage nuclear envelope A sub-cellular barrier consisting of two ...
Alternatively the proteins may react with ligands like lectins giving rise to the term affinity blotting. Western blotting SDS- ... Protein (Western) Blotting - Introduction to Antibodies Membrane Transfer Detailed electroblotting to PVDF procedure - Protein ... Following electrophoresis, a standard tank or semi-dry blotting transfer system is set up. A stack is put together in the ... Because the proteins may retain or regain part of their structure during blotting they may react with specific antibodies ...
Low-to-mid-plex techniques: Reporter gene Northern blot Western blot Fluorescent in situ hybridization Reverse transcription ... One of the most commonly used methods is to perform a Western blot against the protein of interest. This gives information on ... The detection process is very similar to that of a Western blot, but by avoiding the gel steps more accurate quantification can ... Moritz, CP (10 February 2020). "40 years Western blotting: A scientific birthday toast". Journal of Proteomics. 212: 103575. ...
Southern blot Western blot Northern blot Southwestern blot Eastern blot Gel electrophoresis SDS-PAGE Chromatography ... These techniques include the western blot (protein detection), the northern blot (RNA detection), the southwestern blot (DNA- ... The northwestern blot, also known as the northwestern assay, is a hybrid analytical technique of the western blot and the ... A related technique, the western blot, is used to detect a protein of interest that involves transferring proteins that are ...
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  • The western blot (sometimes called the protein immunoblot), or western blotting, is a widely used analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample of tissue homogenate or extract. (wikipedia.org)
  • Other related techniques include dot blot analysis, quantitative dot blot, immunohistochemistry and immunocytochemistry, where antibodies are used to detect proteins in tissues and cells by immunostaining, and enzyme-linked immunosorbent assay (ELISA). (wikipedia.org)
  • The western blot is extensively used in biochemistry for the qualitative detection of single proteins and protein-modifications (such as post-translational modifications). (wikipedia.org)
  • Proteins from known HIV-infected cells are separated and blotted on a membrane as above. (wikipedia.org)
  • Western blotting-or immunoblotting-is a technique used to detect, characterize and quantitate proteins. (bdbiosciences.com)
  • The separated proteins are then transferred or blotted to a nitrocellulose or a PVDF membrane to immobilize the protein. (bdbiosciences.com)
  • Using western blotting, proteins can be characterized for several aspects, including their presence or absence, abundance, phosphorylation status or localization. (bdbiosciences.com)
  • In addition to these qualitative aspects, western blotting can also be used for relative quantitation of proteins based on the band intensity between experimental and control samples. (bdbiosciences.com)
  • Blotting is carried out to transfer proteins from the gel to a solid membrane. (ahdiagnostics.se)
  • The effectiveness of the transfer is heavily reliant on the gel acrylamide percentage, the molecular weight of electrophoresed proteins, and the selected blotting membrane. (ahdiagnostics.se)
  • The S canLater Western Blot Protein Ladder consists of seven biotinylated recombinant proteins as well as three prestained marker proteins. (moleculardevices.com)
  • Detection of the biotinylated proteins is enabled by adding Europium-labeled streptavidin to the blot at the secondary antibody step. (moleculardevices.com)
  • The ScanLater Western Blot Protein Ladder consists of seven biotinylated recombinant proteins at 10, 20, 40, 50, 80, 100 and 140 kD. (moleculardevices.com)
  • White bands are biotinylated protein used to estimate molecular weights of blotted proteins. (moleculardevices.com)
  • The migration distances of seven proteins in the ScanLater Western Blot Protein Ladder are plotted and fitted as a function of their respective molecular weights. (moleculardevices.com)
  • Using western blotting for verification can be a helpful tool, but correct interpretation of the results is crucial to avoid misidentification of cell lines with functional proteins as knockouts (and vice versa). (horizondiscovery.com)
  • The chemiPRO is our complete solution, featuring a highly sensitive cooled CCD camera with high quantum efficiency, for detection of picogram level proteins, this system can image both conventional ECL style chemiluminescence blots and fluorescently tagged proteins of antibodies with the optional fluorescence module. (cleaverscientific.com)
  • Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring specific target proteins in a sample. (labroots.com)
  • Western blotting is an important technique used to identify specific proteins from a complex mixture of proteins extracted from cells or body fluids. (lumigen.com)
  • Lumigen ® has a range of sensitive substrates for the detection of Western blotted proteins with sustained signal duration, reduced antibody usage and rapid onset of chemiluminescence. (lumigen.com)
  • Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes. (wakehealth.edu)
  • Western blotting is a decades-old laboratory technique that is used to detect specific proteins from cell culture, tissue, or blood specimen. (championsoncology.com)
  • Since its initial description, western blotting has been used in all fields of biological and biomedical research because it is a straightforward and robust method for detecting specific proteins. (championsoncology.com)
  • Western blot allows researchers to determine and quantify the proteins existent within a cell, identifying specific proteins out of the mixture of proteins that are present in cell samples. (news-medical.net)
  • Before Western Blotting, we had to prepare the samples, which consisted of many procedures- the last of which was reducing and denaturing the samples by boiling the proteins. (becausementalhealth.org)
  • After the Western Blot was finished, we had to transfer the proteins from the gel to a membrane for future analysis. (becausementalhealth.org)
  • With direct detection, primary antibodies are conjugated directly to an enzyme, like HRP , for chemiluminescent detection or to fluorochromes including Alexa Fluor® dyes, Janelia Fluor® dyes or DyLight® dyes for fluorescent Western blotting. (novusbio.com)
  • Both monoclonal and polyclonal antibodies are used for immunoprobing of antigens in western blotting. (bdbiosciences.com)
  • The tagged antibodies are detected directly (direct western blotting) or using secondary antibodies (indirect western blotting). (bdbiosciences.com)
  • The IgG -WB was done in house and the test was considered positive if the child had antibodies that recognized at least one band on IgG blots different from the mother 's or with greater intensity than the corresponding maternal band, during the first three months of life . (bvsalud.org)
  • The Western blot test detects antimicrobial antibodies in the patient's sample (eg, serum, other body fluid) by their reaction with target antigens (eg, viral components) that have been immobilized onto a membrane by blotting. (msdmanuals.com)
  • This application note describes two methods that allow you to complete the entire western blot workflow in less than 2-5 hours, depending on which time-saving steps you incorporate. (flinnsci.com)
  • The ScanLater Western Blot workflow follows standard gel loading and blotting methods up to the secondary antibody incubation step. (moleculardevices.com)
  • Testing was also performed using an in-house anti-HEV Western Blot assay. (cdc.gov)
  • Further testing with the Wantai HEV-IgG ELISA assay and an in-house anti-HEV Western Blot assay was performed on a subset of 1803 of the 6000 samples. (cdc.gov)
  • Our cutting-edge Western Blotting assay consists of a capillary electrophoresis-based immunoassay for protein detection and quantitation that delivers sharper images and more reproducible results. (championsoncology.com)
  • To support your research needs, we offer several options to customize and integrate our Western Blotting assay. (championsoncology.com)
  • These days, immunohistochemical staining is just one of several established immunostaining techniques, including enzyme-linked immunosorbent assay, flow cytometry, immuno-electron microscopy, and Western blotting. (news-medical.net)
  • I worked alongside two of the lab members in doing Protein Assay to prepare for the Western Blot the next week. (becausementalhealth.org)
  • Title : Interpretation and Use of the Western Blot Assay for Serodiagnosis of Human Immunodeficiency Virus Type 1 Infections Corporate Authors(s) : Centers for Disease Control (U.S.) Published Date : July 21, 1989 Source : MMWR. (cdc.gov)
  • The Trans-Blot Turbo Transfer System is a high-performance western blotting transfer system designed to provide rapid transfers with high efficiency. (bio-rad.com)
  • Add the Trans-Blot Turbo Transfer System of your choice from the list below to your cart and enter promo code 25OFFTRANSBLOT in the promotion code field to receive your discount. (bio-rad.com)
  • A semi-quantitative estimation of a protein can be derived from the size and color intensity of a protein band on the blot membrane. (wikipedia.org)
  • The blotting is performed by assembling filter papers, a membrane, and a gel into a sandwich. (ahdiagnostics.se)
  • Western blotting versus ELISA: What are the differences? (bdbiosciences.com)
  • Like in western blotting, protein detection using an ELISA also uses antibody binding to antigens and could be accomplished with one antibody (direct ELISA) or using a primary and a secondary antibody (indirect ELISA). (bdbiosciences.com)
  • Crude antigenic preparations from Setaria equina were used in ELISA and Western blotting to examine cross-reaction with human sera from areas endemic for bancroftian filariasis. (who.int)
  • Des préparations d'antigènes bruts de Setaria equina ont été utilisées dans le cadre des méthodes ELISA et transfert Western afin d'étudier la réaction croisée avec des sérums humains en provenance de zones endémiques pour la filariose de Bancroft. (who.int)
  • If the ELISA test is positive, it must be confirmed with another test called the Western blot test. (medlineplus.gov)
  • A positive ELISA result must be followed up with a Western blot test. (medlineplus.gov)
  • The Western blot typically has good sensitivity, although often less than that of screening tests such as ELISA, but generally is highly specific. (msdmanuals.com)
  • Researchers use fluorescent western blotting to quantify multiple targets on a single blot. (the-scientist.com)
  • In a previous post, I talked about immunostaining and touched on the principles and procedures of Western blotting, a technique that is widely used to quantify protein expression. (metheno.com)
  • Use Hi LEDs for multiplexing RGB and IR fluorescent blots and gels, UV transilluminator for DNA/RNA gels, visible light converter screen for Coomassie blue and silver stained protein gels and blue converter screen for imaging 'safe' dyes. (syngene.com)
  • This video will show you how to adjust the image appearance of your chemiluminescent Western blot with Image Studio 4.0 Software on the C-DiGit Blot Scanner. (licor.com)
  • These systems can handle fluorescence 1D and 2D gels as well as chemiluminescent blots. (syngene.com)
  • Date: October 14, 2021 Time: 9:00am (PDT), 12:00pm (EDT), 5:00pm GMT Western blot analysis is a method widely used in the lab today because of its versatility in detecting and measuring spe. (labroots.com)
  • Western Blot is a widely used, essential, and trusted technique for protein analysis of complex systems. (championsoncology.com)
  • The final immunostaining method is the Western blot method, a widely used technique that has firmly ingrained itself in the fields of cell and molecular biology. (news-medical.net)
  • However, in recent years the reproducibility and quality of western blotting data has been increasingly scrutinized. (labroots.com)
  • This webinar series will share tips and provide step-by-step guidance on how to improve the quality and reproducibility of data for both qualitative and quantitative western blotting, with attention on some of the newer guidelines. (labroots.com)
  • Champions Oncology provides Western Blotting protein detection assays, developed on the JESS ProteinSimple Analyzer, for higher data quality and reproducibility. (championsoncology.com)
  • These blue bands are seen on the electrophoresis gel and on the blot after transfer. (moleculardevices.com)
  • In this webinar, Kelly Wolfe will discuss the dos and don'ts of protein electrophoresis and western blotting. (the-scientist.com)
  • In this webinar, R. Hal Scofield discusses hints and tips on how to generate clean and reproducible western blot data, even in tricky situations. (the-scientist.com)
  • In this webinar, Kelly Wolfe will discuss innovations that streamline western blotting workflows. (the-scientist.com)
  • In this webinar, Aldrin Gomes will reveal how to obtain reproducible, publication-ready western blot images. (the-scientist.com)
  • Our team of western blotting experts have put together this 3-part webinar series that is designed to help you avoid some of the pitfalls associated with western blotting and enable you to achieve publication-quality western blot results. (labroots.com)
  • Knockout Validated Western Blot showing lysates of HeLa parental cell line and LC3B knockout HeLa cell line (KO) untreated (-) or treated (+) with 50 uM Chloroquine. (novusbio.com)
  • No attempts have been made to qualify this product on blots made with HeLa cell, or any other antigen source. (calbiotech.com)
  • Membranes are scanned on either the SpectraMax Paradigm or SpectraMax i3 Multi-Mode Microplate Reader with the ScanLater Western Blot Detection Cartridge,which utilizes time resolved fluorescence (TRF) detection of Europium (Eu). (moleculardevices.com)
  • Europium is resistant to photo-bleaching, so the western blot signal remains stable for weeks to months which enables repeated reading of membranes. (moleculardevices.com)
  • Blotting membranes are supplied in sheet form and as a 3m roll which can be cut to size. (cleaverscientific.com)
  • Western blot and Wantai IgG anti-HEV testing was performed to determine correlation with the DSI and Kuniholm NIH in-house IgG anti-HEV assays. (cdc.gov)
  • At Champions, we provide Western Blotting protein detection assays, optimized on the Jess ProteinSimple Analyzer. (championsoncology.com)
  • Following sample prep, you need to do polyacrylamide gel electrophoresis (PAGE) and blotting. (ahdiagnostics.se)
  • The term "western blot" was given by W. Neal Burnette in 1981, although the method itself was independently invented in 1979 by Jaime Renart, Jakob Reiser, and George Stark at Stanford University, and by Harry Towbin, Theophil Staehelin, and Julian Gordon at the Friedrich Miescher Institute in Basel, Switzerland. (wikipedia.org)
  • 2013). 'Detection of cathepsin D in ewe's milk by Western Blotting method', Mljekarstvo , 63(1), str. (srce.hr)
  • Dolenčić Špehar I, Martinković F, Havranek J, Marinculić A, Tudor Kalit M, Kalit S. Detection of cathepsin D in ewe's milk by Western Blotting method. (srce.hr)
  • This paper shows the presence of cathepsin D and its forms in ewe's milk by modified Western Blotting method. (srce.hr)
  • At the final step of Western blotting you will need to choose a detection method to visualize your results. (ahdiagnostics.se)
  • In this blog we provide an overview of the western blot method and highlight its current applications in preclinical oncology research. (championsoncology.com)
  • Evaluation of the Western blotting method for the diagnosis of congenital toxoplasmosis. (bvsalud.org)
  • To evaluate the Western blotting method for the detection of IgG anti- Toxoplasma gondii (T. gondii) ( IgG -WB) in the serum of children with suspected congenital toxoplasmosis . (bvsalud.org)
  • Some forms of Lyme disease testing employ western blotting. (wikipedia.org)
  • Only a positive Western blot test can confirm the diagnosis of Lyme disease. (medlineplus.gov)
  • With boxes that are correctly matched to common blot sizes, you can fit more blots on shakers/rockers while keeping reagent and antibody usage to an absolute minimum. (thomassci.com)
  • Faster and more efficient than other western blotting transfer methods. (bio-rad.com)
  • The confirmatory HIV test employs a western blot to detect anti-HIV antibody in a human serum sample. (wikipedia.org)
  • Western blot sample preparations, including lysis buffers, lysate from cell culture, lysate from tissues and determination of protein concentration. (abcam.com)
  • We have gel loading tips, sample loading buffers, protein ladders, transfer buffers, and much more for the protein separation and blotting steps of Western blotting. (ahdiagnostics.se)
  • A robust, automated western blot system accelerates immunotherapy development. (the-scientist.com)
  • The ScanLater ™ Western Blot Protein Ladder is an essential component of the ScanLater ™ Western Blot Detection System. (moleculardevices.com)
  • They are also seen as dark bands when the ladder is read with the Scanlater Western Blot Detection System. (moleculardevices.com)
  • They are detected after incubation with Eu-streptavidin and read on the ScanLater Western Blot Detection System. (moleculardevices.com)
  • The ScanLater Western Blot Detection System, including the ScanLater Western Blot Protein Ladder, is a simple, sensitive, and stable platform that provides excellent protein analysis capability in a multi-mode microplate reader. (moleculardevices.com)
  • Whether you are looking to move out of the darkroom and invest in your first digital imaging system/Western blot imager or if you are looking to replace an old system, it is important to purchase the right equipment that will adapt with your research. (syngene.com)
  • Then maximise your space and budget with an imaging system/Western blot imaging system that is versatile and capable of imaging a wide range of applications in one system. (syngene.com)
  • Whichever gel imaging system/Western blot imaging system you choose, you want a system that is intuitive and can be used by everyone without training. (syngene.com)
  • GeneSys image capture software from Syngene makes imaging gels and western blots simple by using system protocols - just place your sample in the darkroom, position your sample and click the capture button for high quality images. (syngene.com)
  • Implementing a gel imaging system/Western blot imager eliminates the chemical waste associated with film processing. (syngene.com)
  • Select a Western blot imaging system that uses LEDs as they are environmentally friendly. (syngene.com)
  • System is supplied complete with power leads and four blotting cassetes. (transilluminators.com)
  • Review tips to guide all stages of western blotting, from sample preparation to imaging and analysis. (the-scientist.com)
  • Users commonly look at the sensor resolution of the camera when evaluating gel documentation imaging systems/Western blot imagers. (syngene.com)
  • There were many different uses for the samples- DNA Extraction, Western Blotting and Imaging- and we were taught the many different ways to get the samples for them. (becausementalhealth.org)
  • Antibody against human SS-A (Ro), detectable bands at SS-A 60kD by Western blot immunoassay at 1:100 dilution using Hep-2 cellular extract blot. (calbiotech.com)
  • With a range of Vertical Gel Systems, Power Supplies, Blot Transfer Systems, Reagents and Chemicals and Gel Documentation and Analysis, we provide the complete solution for the Life Scientist. (cleaverscientific.com)
  • Your choice will influence the protocol and chemistry for your Western blot, depending on the type of information you are looking for. (ahdiagnostics.se)
  • Further applications of the western blot technique include its use by the World Anti-Doping Agency (WADA). (wikipedia.org)
  • The primary applications of this protein ladder include the estimation of protein molecular weight, the visualization of gel electrophoresis, and the evaluation of the transfer process from gel to blot. (moleculardevices.com)
  • On the basis of application, the western blotting market is divided into clinical diagnostics, agricultural applications, biomedical research, and some other related applications. (tmrresearchblog.com)
  • from preparation to separation and blotting as well as antibody incubation and, lastly, detection. (ahdiagnostics.se)
  • The western blot is routinely used for verification of protein production after cloning. (wikipedia.org)
  • Results of search for 'su:{Blotting, Western. (who.int)
  • Our portfolio for Western blotting is full of products from leading manufacturers. (ahdiagnostics.se)
  • These growth prospects are attributed to the growing technological advancements in the products from the western blotting market. (tmrresearchblog.com)
  • Furthermore, these products from the western blotting market are also used in order to reduce the processing time of western blotting. (tmrresearchblog.com)
  • So, owing to these advanced products and their features, the western blotting market is likely to grow at a significant speed in the forthcoming years. (tmrresearchblog.com)
  • Western blot technique uses three elements to achieve its task of separating a specific protein from a complex: separation by size, transfer of protein to a solid support, and marking target protein using a primary and secondary antibody to visualize. (wikipedia.org)
  • The western blotting market is expected to experience various growth opportunities in the following years. (tmrresearchblog.com)
  • On the basis of these initiatives, the western blotting market is likely to experience innumerable growth avenues in the upcoming years. (tmrresearchblog.com)
  • This report examines the variation in Western blot (WB) interpretive criteria reported by laboratories enrolled in CDC's Model Performance Evaluation Program (MPEP) for HIV-1-antibody testing. (cdc.gov)
  • Our expert team can help you integrate Western Blotting analysis in your preclinical studies or clinical specialty testing for target validation, model and sample characterization, detection of post-translational modification, and much more. (championsoncology.com)
  • A western blot can also be used as a confirmatory test for Hepatitis B infection and HSV-2 (Herpes Type 2) infection. (wikipedia.org)
  • It is important to prepare the starting material properly in order to obtain a successful Western blot. (ahdiagnostics.se)
  • Dark bands are pre-stained protein used to monitor progress of protein electrophoresis, blot orientation, and assess transfer efficiency. (moleculardevices.com)
  • Western blot highlighting GAPDH expression in lysates of human brain, mouse brain, and rat brain tissue. (novusbio.com)
  • The name western blot is a play on the Southern blot, a technique for DNA detection named after its inventor, English biologist Edwin Southern. (wikipedia.org)
  • The western blot technique was used during the 2014 FIFA World Cup in the anti-doping campaign for that event. (wikipedia.org)
  • Western blotting is a simple and straightforward technique for protein characterization. (ahdiagnostics.se)
  • Recently, I've been working as a research assistant in a lab and finally came across the technique of Western blot. (metheno.com)