Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation.
The attachment of PLATELETS to one another. This clumping together can be induced by a number of agents (e.g., THROMBIN; COLLAGEN) and is part of the mechanism leading to the formation of a THROMBUS.
The number of PLATELETS per unit volume in a sample of venous BLOOD.
The process whereby PLATELETS adhere to something other than platelets, e.g., COLLAGEN; BASEMENT MEMBRANE; MICROFIBRILS; or other "foreign" surfaces.
Surface glycoproteins on platelets which have a key role in hemostasis and thrombosis such as platelet adhesion and aggregation. Many of these are receptors.
A CXC chemokine that is found in the alpha granules of PLATELETS. The protein has a molecular size of 7800 kDa and can occur as a monomer, a dimer or a tetramer depending upon its concentration in solution. Platelet factor 4 has a high affinity for HEPARIN and is often found complexed with GLYCOPROTEINS such as PROTEIN C.
An enzyme formed from PROTHROMBIN that converts FIBRINOGEN to FIBRIN.
Adenosine 5'-(trihydrogen diphosphate). An adenine nucleotide containing two phosphate groups esterified to the sugar moiety at the 5'-position.
The transfer of blood platelets from a donor to a recipient or reinfusion to the donor.
A biochemical messenger and regulator, synthesized from the essential amino acid L-TRYPTOPHAN. In humans it is found primarily in the central nervous system, gastrointestinal tract, and blood platelets. Serotonin mediates several important physiological functions including neurotransmission, gastrointestinal motility, hemostasis, and cardiovascular integrity. Multiple receptor families (RECEPTORS, SEROTONIN) explain the broad physiological actions and distribution of this biochemical mediator.
Platelet membrane glycoprotein complex important for platelet adhesion and aggregation. It is an integrin complex containing INTEGRIN ALPHAIIB and INTEGRIN BETA3 which recognizes the arginine-glycine-aspartic acid (RGD) sequence present on several adhesive proteins. As such, it is a receptor for FIBRINOGEN; VON WILLEBRAND FACTOR; FIBRONECTIN; VITRONECTIN; and THROMBOSPONDINS. A deficiency of GPIIb-IIIa results in GLANZMANN THROMBASTHENIA.
Platelet membrane glycoprotein complex essential for normal platelet adhesion and clot formation at sites of vascular injury. It is composed of three polypeptides, GPIb alpha, GPIb beta, and GPIX. Glycoprotein Ib functions as a receptor for von Willebrand factor and for thrombin. Congenital deficiency of the GPIb-IX complex results in Bernard-Soulier syndrome. The platelet glycoprotein GPV associates with GPIb-IX and is also absent in Bernard-Soulier syndrome.
Very large BONE MARROW CELLS which release mature BLOOD PLATELETS.
A platelet-specific protein which is released when platelets aggregate. Elevated plasma levels have been reported after deep venous thrombosis, pre-eclampsia, myocardial infarction with mural thrombosis, and myeloproliferative disorders. Measurement of beta-thromboglobulin in biological fluids by radioimmunoassay is used for the diagnosis and assessment of progress of thromboembolic disorders.
A series of progressive, overlapping events, triggered by exposure of the PLATELETS to subendothelial tissue. These events include shape change, adhesiveness, aggregation, and release reactions. When carried through to completion, these events lead to the formation of a stable hemostatic plug.
Disorders caused by abnormalities in platelet count or function.
A subnormal level of BLOOD PLATELETS.
A phospholipid derivative formed by PLATELETS; BASOPHILS; NEUTROPHILS; MONOCYTES; and MACROPHAGES. It is a potent platelet aggregating agent and inducer of systemic anaphylactic symptoms, including HYPOTENSION; THROMBOCYTOPENIA; NEUTROPENIA; and BRONCHOCONSTRICTION.
Laboratory examination used to monitor and evaluate platelet function in a patient's blood.
Plasma glycoprotein clotted by thrombin, composed of a dimer of three non-identical pairs of polypeptide chains (alpha, beta, gamma) held together by disulfide bonds. Fibrinogen clotting is a sol-gel change involving complex molecular arrangements: whereas fibrinogen is cleaved by thrombin to form polypeptides A and B, the proteolytic action of other enzymes yields different fibrinogen degradation products.
2-Octylcyclopentaneheptanoic acids. The family of saturated carbon-20 cyclic fatty acids that represent the parent compounds of the prostaglandins.
Cell adhesion molecule and CD antigen that mediates the adhesion of neutrophils and monocytes to activated platelets and endothelial cells.
A high-molecular-weight plasma protein, produced by endothelial cells and megakaryocytes, that is part of the factor VIII/von Willebrand factor complex. The von Willebrand factor has receptors for collagen, platelets, and ristocetin activity as well as the immunologically distinct antigenic determinants. It functions in adhesion of platelets to collagen and hemostatic plug formation. The prolonged bleeding time in VON WILLEBRAND DISEASES is due to the deficiency of this factor.
The prototypical analgesic used in the treatment of mild to moderate pain. It has anti-inflammatory and antipyretic properties and acts as an inhibitor of cyclooxygenase which results in the inhibition of the biosynthesis of prostaglandins. Aspirin also inhibits platelet aggregation and is used in the prevention of arterial and venous thrombosis. (From Martindale, The Extra Pharmacopoeia, 30th ed, p5)
The process of generating thrombocytes (BLOOD PLATELETS) from the pluripotent HEMATOPOIETIC STEM CELLS in the BONE MARROW via the MEGAKARYOCYTES. The humoral factor with thrombopoiesis-stimulating activity is designated THROMBOPOIETIN.
A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).
The number of LEUKOCYTES and ERYTHROCYTES per unit volume in a sample of venous BLOOD. A complete blood count (CBC) also includes measurement of the HEMOGLOBIN; HEMATOCRIT; and ERYTHROCYTE INDICES.
An unstable intermediate between the prostaglandin endoperoxides and thromboxane B2. The compound has a bicyclic oxaneoxetane structure. It is a potent inducer of platelet aggregation and causes vasoconstriction. It is the principal component of rabbit aorta contracting substance (RCS).
The process of the interaction of BLOOD COAGULATION FACTORS that results in an insoluble FIBRIN clot.
The process which spontaneously arrests the flow of BLOOD from vessels carrying blood under pressure. It is accomplished by contraction of the vessels, adhesion and aggregation of formed blood elements (eg. ERYTHROCYTE AGGREGATION), and the process of BLOOD COAGULATION.
Human alloantigens expressed only on platelets, specifically on platelet membrane glycoproteins. These platelet-specific antigens are immunogenic and can result in pathological reactions to transfusion therapy.
Formation and development of a thrombus or blood clot in the blood vessel.
A humoral factor that stimulates the production of thrombocytes (BLOOD PLATELETS). Thrombopoietin stimulates the proliferation of bone marrow MEGAKARYOCYTES and their release of blood platelets. The process is called THROMBOPOIESIS.
Condensed areas of cellular material that may be bounded by a membrane.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
An opioid analgesic chemically related to and with an action resembling that of MEPERIDINE, but more rapid in onset and of shorter duration. It has been used in obstetrics, as pre-operative medication, for minor surgical procedures, and for dental procedures. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1067)
Physiologically active compounds found in many organs of the body. They are formed in vivo from the prostaglandin endoperoxides and cause platelet aggregation, contraction of arteries, and other biological effects. Thromboxanes are important mediators of the actions of polyunsaturated fatty acids transformed by cyclooxygenase.
A mixture of the mesylates (methane sulfonates) of DIHYDROERGOCORNINE; DIHYDROERGOCRISTINE; and the alpha- and beta-isomers of DIHYDROERGOCRYPTINE. The substance produces a generalized peripheral vasodilation and a fall in arterial pressure and has been used to treat symptoms of mild to moderate impairment of mental function in the elderly.
A phosphodiesterase inhibitor which inhibits platelet aggregation. Formerly used as an antineoplastic.
The process by which blood or its components are kept viable outside of the organism from which they are derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).
A plant genus of the family ROSACEAE. The common names of chokeberry or chokecherry are also used for some species of PRUNUS.
A deficiency or absence of FIBRINOGEN in the blood.
Duration of blood flow after skin puncture. This test is used as a measure of capillary and platelet function.
A vasoconstrictor found in ergot of Central Europe. It is a serotonin agonist that has been used as an oxytocic agent and in the treatment of MIGRAINE DISORDERS.
A tricyclic antidepressant that has actions and uses similar to those of AMITRIPTYLINE, but has only weak antimuscarinic and sedative effects. (From Martindale, The Extra Pharmacopoeia, 30th ed, p257)
Cell surface proteins that bind THROMBOXANES with high affinity and trigger intracellular changes influencing the behavior of cells. Some thromboxane receptors act via the inositol phosphate and diacylglycerol second messenger systems.
The rate dynamics in chemical or physical systems.
Proteins that are present in blood serum, including SERUM ALBUMIN; BLOOD COAGULATION FACTORS; and many other types of proteins.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
The active sympathomimetic hormone from the ADRENAL MEDULLA. It stimulates both the alpha- and beta- adrenergic systems, causes systemic VASOCONSTRICTION and gastrointestinal relaxation, stimulates the HEART, and dilates BRONCHI and cerebral vessels. It is used in ASTHMA and CARDIAC FAILURE and to delay absorption of local ANESTHETICS.
Platelet membrane glycoprotein IIb is an integrin alpha subunit that heterodimerizes with INTEGRIN BETA3 to form PLATELET GLYCOPROTEIN GPIIB-IIIA COMPLEX. It is synthesized as a single polypeptide chain which is then postranslationally cleaved and processed into two disulfide-linked subunits of approximately 18 and 110 kDa in size.
A prostaglandin that is a powerful vasodilator and inhibits platelet aggregation. It is biosynthesized enzymatically from PROSTAGLANDIN ENDOPEROXIDES in human vascular tissue. The sodium salt has been also used to treat primary pulmonary hypertension (HYPERTENSION, PULMONARY).
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
A phospholipid from the platelet membrane that contributes to the blood clotting cascade by forming a phospholipid-protein complex (THROMBOPLASTIN) which serves as a cofactor with FACTOR VIIA to activate FACTOR X in the extrinsic pathway of BLOOD COAGULATION.
The prototypical tricyclic antidepressant. It has been used in major depression, dysthymia, bipolar depression, attention-deficit disorders, agoraphobia, and panic disorders. It has less sedative effect than some other members of this therapeutic group.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
An organization of cells into an organ-like structure. Organoids can be generated in culture. They are also found in certain neoplasms.
Hemorrhagic and thrombotic disorders that occur as a consequence of abnormalities in blood coagulation due to a variety of factors such as COAGULATION PROTEIN DISORDERS; BLOOD PLATELET DISORDERS; BLOOD PROTEIN DISORDERS or nutritional conditions.
The network of filaments, tubules, and interconnecting filamentous bridges which give shape, structure, and organization to the cytoplasm.
An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.
Drugs or agents which antagonize or impair any mechanism leading to blood platelet aggregation, whether during the phases of activation and shape change or following the dense-granule release reaction and stimulation of the prostaglandin-thromboxane system.
Elements of limited time intervals, contributing to particular results or situations.
A group of compounds derived from unsaturated 20-carbon fatty acids, primarily arachidonic acid, via the cyclooxygenase pathway. They are extremely potent mediators of a diverse group of physiological processes.
An ionophorous, polyether antibiotic from Streptomyces chartreusensis. It binds and transports CALCIUM and other divalent cations across membranes and uncouples oxidative phosphorylation while inhibiting ATPase of rat liver mitochondria. The substance is used mostly as a biochemical tool to study the role of divalent cations in various biological systems.
An unsaturated, essential fatty acid. It is found in animal and human fat as well as in the liver, brain, and glandular organs, and is a constituent of animal phosphatides. It is formed by the synthesis from dietary linoleic acid and is a precursor in the biosynthesis of prostaglandins, thromboxanes, and leukotrienes.
Cell surface receptors that are specific for THROMBOPOIETIN. They signal through interaction with JANUS KINASES such as JANUS KINASE 2.
A chelating agent that sequesters a variety of polyvalent cations such as CALCIUM. It is used in pharmaceutical manufacturing and as a food additive.
An ergot derivative that is a congener of LYSERGIC ACID DIETHYLAMIDE. It antagonizes the effects of serotonin in blood vessels and gastrointestinal smooth muscle, but has few of the properties of other ergot alkaloids. Methysergide is used prophylactically in migraine and other vascular headaches and to antagonize serotonin in the carcinoid syndrome.
Field of chemistry that pertains to immunological phenomena and the study of chemical reactions related to antigen stimulation of tissues. It includes physicochemical interactions between antigens and antibodies.
The sum of the weight of all the atoms in a molecule.
A subclass of purinergic P2Y receptors that have a preference for ADP binding and are coupled to GTP-BINDING PROTEIN ALPHA SUBUNIT, GI. The P2Y12 purinergic receptors are found in PLATELETS where they play an important role regulating PLATELET ACTIVATION.
Cell surface receptors that bind prostaglandins with high affinity and trigger intracellular changes which influence the behavior of cells. Prostaglandin receptor subtypes have been tentatively named according to their relative affinities for the endogenous prostaglandins. They include those which prefer prostaglandin D2 (DP receptors), prostaglandin E2 (EP1, EP2, and EP3 receptors), prostaglandin F2-alpha (FP receptors), and prostacyclin (IP receptors).
Organic compounds that contain 1,2-diphenylethylene as a functional group.
Antibodies produced by a single clone of cells.
Disorder characterized by a decrease or lack of platelet dense bodies in which the releasable pool of adenine nucleotides and 5HT are normally stored.
A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts.
An antibiotic mixture of two components, A and B, obtained from Nocardia lurida (or the same substance produced by any other means). It is no longer used clinically because of its toxicity. It causes platelet agglutination and blood coagulation and is used to assay those functions in vitro.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
A congenital bleeding disorder with prolonged bleeding time, absence of aggregation of platelets in response to most agents, especially ADP, and impaired or absent clot retraction. Platelet membranes are deficient in or have a defect in the glycoprotein IIb-IIIa complex (PLATELET GLYCOPROTEIN GPIIB-IIIA COMPLEX).
The relationship between the dose of an administered drug and the response of the organism to the drug.
A protein factor that regulates the length of R-actin. It is chemically similar, but immunochemically distinguishable from actin.
Peptides composed of between two and twelve amino acids.
Any form of purpura in which the PLATELET COUNT is decreased. Many forms are thought to be caused by immunological mechanisms.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.
Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.
A dynamic actin-rich extension of the surface of an animal cell used for locomotion or prehension of food.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
Venoms from snakes of the subfamily Crotalinae or pit vipers, found mostly in the Americas. They include the rattlesnake, cottonmouth, fer-de-lance, bushmaster, and American copperhead. Their venoms contain nontoxic proteins, cardio-, hemo-, cyto-, and neurotoxins, and many enzymes, especially phospholipases A. Many of the toxins have been characterized.
An alkaloid found in the roots of Rauwolfia serpentina and R. vomitoria. Reserpine inhibits the uptake of norepinephrine into storage vesicles resulting in depletion of catecholamines and serotonin from central and peripheral axon terminals. It has been used as an antihypertensive and an antipsychotic as well as a research tool, but its adverse effects limit its clinical use.
A chelating agent relatively more specific for calcium and less toxic than EDETIC ACID.
The quantity of volume or surface area of CELLS.
A layer of epithelium that lines the heart, blood vessels (ENDOTHELIUM, VASCULAR), lymph vessels (ENDOTHELIUM, LYMPHATIC), and the serous cavities of the body.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Increased numbers of platelets in the peripheral blood. (Dorland, 27th ed)
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
Endogenous substances, usually proteins, that are involved in the blood coagulation process.
A preparation consisting of PLATELETS concentrated in a limited volume of PLASMA. This is used in various surgical tissue regeneration procedures where the GROWTH FACTORS in the platelets enhance wound healing and regeneration.
A stable, physiologically active compound formed in vivo from the prostaglandin endoperoxides. It is important in the platelet-release reaction (release of ADP and serotonin).
Adherence of cells to surfaces or to other cells.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Synthetic compounds that are analogs of the naturally occurring prostaglandin endoperoxides and that mimic their pharmacologic and physiologic activities. They are usually more stable than the naturally occurring compounds.
Organic nitrogenous bases. Many alkaloids of medical importance occur in the animal and vegetable kingdoms, and some have been synthesized. (Grant & Hackh's Chemical Dictionary, 5th ed)
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
The number of WHITE BLOOD CELLS per unit volume in venous BLOOD. A differential leukocyte count measures the relative numbers of the different types of white cells.
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.
Stable carbon atoms that have the same atomic number as the element carbon, but differ in atomic weight. C-13 is a stable carbon isotope.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.
The development and formation of various types of BLOOD CELLS. Hematopoiesis can take place in the BONE MARROW (medullary) or outside the bone marrow (HEMATOPOIESIS, EXTRAMEDULLARY).
A familial coagulation disorder characterized by a prolonged bleeding time, unusually large platelets, and impaired prothrombin consumption.
Proteins prepared by recombinant DNA technology.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
A highly polar organic liquid, that is used widely as a chemical solvent. Because of its ability to penetrate biological membranes, it is used as a vehicle for topical application of pharmaceuticals. It is also used to protect tissue during CRYOPRESERVATION. Dimethyl sulfoxide shows a range of pharmacological activity including analgesia and anti-inflammation.
A purely physical condition which exists within any material because of strain or deformation by external forces or by non-uniform thermal expansion; expressed quantitatively in units of force per unit area.
A family of proteinase-activated receptors that are specific for THROMBIN. They are found primarily on PLATELETS and on ENDOTHELIAL CELLS. Activation of thrombin receptors occurs through the proteolytic action of THROMBIN, which cleaves the N-terminal peptide from the receptor to reveal a new N-terminal peptide that is a cryptic ligand for the receptor. The receptors signal through HETEROTRIMERIC GTP-BINDING PROTEINS. Small synthetic peptides that contain the unmasked N-terminal peptide sequence can also activate the receptor in the absence of proteolytic activity.
The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)
A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Drugs that bind to but do not activate serotonin receptors, thereby blocking the actions of serotonin or SEROTONIN RECEPTOR AGONISTS.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
The preparation of platelet concentrates with the return of red cells and platelet-poor plasma to the donor.
A calcium-activated enzyme that catalyzes the hydrolysis of ATP to yield AMP and orthophosphate. It can also act on ADP and other nucleoside triphosphates and diphosphates. EC
Univalent antigen-binding fragments composed of one entire IMMUNOGLOBULIN LIGHT CHAIN and the amino terminal end of one of the IMMUNOGLOBULIN HEAVY CHAINS from the hinge region, linked to each other by disulfide bonds. Fab contains the IMMUNOGLOBULIN VARIABLE REGIONS, which are part of the antigen-binding site, and the first IMMUNOGLOBULIN CONSTANT REGIONS. This fragment can be obtained by digestion of immunoglobulins with the proteolytic enzyme PAPAIN.
A stable prostaglandin endoperoxide analog which serves as a thromboxane mimetic. Its actions include mimicking the hydro-osmotic effect of VASOPRESSIN and activation of TYPE C PHOSPHOLIPASES. (From J Pharmacol Exp Ther 1983;224(1): 108-117; Biochem J 1984;222(1):103-110)
A metallic element, atomic number 49, atomic weight 114.82, symbol In. It is named from its blue line in the spectrum. (From Dorland, 28th ed)
Slender, cylindrical filaments found in the cytoskeleton of plant and animal cells. They are composed of the protein TUBULIN and are influenced by TUBULIN MODULATORS.
Collagen receptors are cell surface receptors that modulate signal transduction between cells and the EXTRACELLULAR MATRIX. They are found in many cell types and are involved in the maintenance and regulation of cell shape and behavior, including PLATELET ACTIVATION and aggregation, through many different signaling pathways and differences in their affinities for collagen isoforms. Collagen receptors include discoidin domain receptors, INTEGRINS, and glycoprotein VI.
Proteins that bind specific drugs with high affinity and trigger intracellular changes influencing the behavior of cells. Drug receptors are generally thought to be receptors for some endogenous substance not otherwise specified.
An absence of warmth or heat or a temperature notably below an accustomed norm.
The deformation and flow behavior of BLOOD and its elements i.e., PLASMA; ERYTHROCYTES; WHITE BLOOD CELLS; and BLOOD PLATELETS.
The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Thrombocytopenia occurring in the absence of toxic exposure or a disease associated with decreased platelets. It is mediated by immune mechanisms, in most cases IMMUNOGLOBULIN G autoantibodies which attach to platelets and subsequently undergo destruction by macrophages. The disease is seen in acute (affecting children) and chronic (adult) forms.
A family of related, adhesive glycoproteins which are synthesized, secreted, and incorporated into the extracellular matrix of a variety of cells, including alpha granules of platelets following thrombin activation and endothelial cells. They interact with a number of BLOOD COAGULATION FACTORS and anticoagulant factors. Five distinct forms have been identified, thrombospondin 1, -2, -3, -4, and cartilage oligomeric matrix protein (COMP). They are involved in cell adhesion, platelet aggregation, cell proliferation, angiogenesis, tumor metastasis, VASCULAR SMOOTH MUSCLE growth, and tissue repair.
A rare, inherited platelet disorder characterized by a selective deficiency in the number and contents of platelet alpha-granules. It is associated with THROMBOCYTOPENIA, enlarged platelets, and prolonged bleeding time.
Bleeding or escape of blood from a vessel.

Values of three coagulation screening tests of precolostral calves. (1/11030)

Prothrombin times, partial thromboplastin times and platelet counts were performed to determine normal values and to screen for coagulation defects of precolostral calves. The precolostral calves were in two groups: one group of a few calves was tested two years before the second larger group. The results for both groups were similar. The tests were performed on postcolostral calves and on mature cows to compare their values with those of precolostral calves. The mean values of prothrombin times and partial thromboplastin times of precolostral calves in the first group were 18.8 seconds and 54.8 seconds respectively. The mean values of prothrombin times and partial thromboplastin times of precolostral calves in the second group were 18.8 seconds and 50.8 seconds respectively. The mean platelet count was 422,400/cmm for the first group and 482,800/cmm for the second group.  (+info)

Enhanced myocardial glucose use in patients with a deficiency in long-chain fatty acid transport (CD36 deficiency). (2/11030)

CD36 is a multifunctional, 88 kDa glycoprotein that is expressed on platelets and monocytes/macrophages. CD36 also has high homology with the long-chain fatty acid (LFA) transporter in the myocardium. Although platelet and monocyte CD36 levels can indicate a CD36 deficiency, they cannot predict specific clinical manifestations in the myocardium of a given person. We examined the hypothesis that a deficiency in LFA transport augments myocardial glucose uptake in patients with a type I CD36 deficiency. METHODS: Seven fasting patients with a type I CD36 deficiency and 9 controls were assessed by cardiac radionuclide imaging using beta-methyl-p-iodophenyl-pentadecanoic acid (BMIPP) as a LFA tracer and by PET with 18F-fluorodeoxyglucose (FDG). RESULTS: None of the patients with a CD36 deficiency showed myocardial uptake of BMIPP. The percentage dose uptake of BMIPP in these subjects was significantly lower than that in normal controls (1.31+/-0.24 versus 2.90+/-0.2; P < 0.005). PET studies revealed that myocardial FDG accumulation was substantially increased in patients with a CD36 deficiency. Quantitative analysis showed that the percentage dose uptake of FDG in patients with a CD36 deficiency was significantly higher than that in normal controls (1.28+/-0.35 versus 0.43+/-0.22; P< 0.01). CONCLUSION: CD36 functions as a major myocardial LFA transporter and its absence may cause a compensatory upregulation of myocardial glucose uptake.  (+info)

Tyrosine phosphorylation of SLP-76 is downstream of Syk following stimulation of the collagen receptor in platelets. (3/11030)

Collagen-related peptide (CRP), a collagen homologue, induces platelet activation through a tyrosine kinase-dependent pathway, leading to sequential tyrosine phosphorylation of Fc receptor (FcR) gamma-chain, Syk, and phospholipase C-gamma2. Here we report that CRP and the platelet low affinity immune receptor FcgammaRIIA stimulate tyrosine phosphorylation of the T cell adapter SLP-76, whereas the G protein-coupled receptor agonist thrombin induces only minor tyrosine phosphorylation. This suggests that SLP-76 has a specific role downstream of receptors that signal via an immunoreceptor tyrosine-based activation motif. Immunoprecipitation studies demonstrate association of SLP-76 with SLAP-130, Vav, Fyn, Lyn, and the FcR gamma-chain in CRP-stimulated platelets. Several of these proteins, including SLP-76, undergo tyrosine phosphorylation in in vitro kinase assays performed on SLP-76 immunoprecipitates. Tyrosine phosphorylation of all of these proteins in the in vitro kinase assay was abrogated by the Src family kinase inhibitor PP1, suggesting that it is mediated by either Fyn or Lyn. The physiological significance of this is uncertain, however, since tyrosine phosphorylation of SLP-76 in vivo is not altered in either Fyn- or Lyn-deficient platelets. CRP stimulation of Syk-deficient platelets demonstrated that in vivo tyrosine phosphorylation of SLP-76 is downstream of Syk. The absence of Syk in the SLP-76 immunoprecipitates raises the possibility that another protein is responsible for bringing SLP-76 to Syk. Candidates for this include those proteins that co-immunoprecipitate with SLP-76, including the FcR gamma-chain. Tyrosine phosphorylation of PLC-gamma2 and Ca2+ mobilization is markedly attenuated in SLP-76-deficient platelets following CRP stimulation, suggesting that the adapter plays a critical role in the regulation of the phospholipase. The increase in tyrosine phosphorylation of SLAP-130 in response to CRP is also inhibited in SLP-76-deficient platelets, placing it downstream of SLP-76. This work identifies SLP-76 as an important adapter molecule that is regulated by Syk and lies upstream of SLAP-130 and PLC-gamma2 in CRP-stimulated platelets.  (+info)

Changes in haematological parameters and iron metabolism associated with a 1600 kilometre ultramarathon. (4/11030)

OBJECTIVE: To investigate haematological variations and iron related changes in the serum of participants in a 1600 kilometre ultramarathon run. PARTICIPANTS: Seven male and two female participants in a 1600 km foot race. METHODS: Blood samples were obtained from the participants before, after four and 11 days of running, and at the end of the event. Samples were analysed by standard methods for haemoglobin, packed cell volume, total red cell count, mean red cell volume, mean red cell haemoglobin, total white cell count and differential, platelets, reticulocytes, iron, ferritin, total iron binding capacity, percentage transferrin saturation, haptoglobin, and bilirubin and corrected for changes in plasma volume. RESULTS: The following variables decreased during the event (p < 0.05): haemoglobin, packed cell volume, mean red cell volume, percentage lymphocytes, percentage monocytes, serum iron, total iron binding capacity, and percentage transferrin saturation. Increases (p < 0.05) were found in plasma volume, total red cell count (day 4 only), total white cell count, percentage and absolute numbers of neutrophils and reticulocytes, absolute numbers of lymphocytes and monocytes (day 4 only), absolute numbers of eosinophils (day 11 and race end), absolute numbers of basophils (race end only), platelets, ferritin, haptoglobin, and bilirubin (day 4 only). CONCLUSION: Ultramarathon running is associated with a wide range of changes in haematological parameters, many of which are related to the normal acute phase response to injury. These should not be confused with indicators of disease.  (+info)

The Megakaryocyte/Platelet-specific enhancer of the alpha2beta1 integrin gene: two tandem AP1 sites and the mitogen-activated protein kinase signaling cascade. (5/11030)

The alpha2beta1 integrin, a collagen receptor on platelets and megakaryocytes, is required for normal platelet function. Transcriptional regulation of the alpha2 integrin gene in cells undergoing megakaryocytic differentiation requires a core promoter between bp -30 and -92, a silencer between bp -92 and -351, and megakaryocytic enhancers in the distal 5' flank. We have now identified a 229-bp region of the distal 5' flank of the alpha2 integrin gene required for high-level enhancer activity in cells with megakaryocytic features. Two tandem AP1 binding sites with dyad symmetry are required for enhancer activity and for DNA-protein complex formation with members of the c-fos/c-jun family. The requirement for AP1 activation suggested a role for the mitogen-activated protein kinase (MAPK) signaling pathway in regulating alpha2 integrin gene expression. Inhibition of the MAP kinase cascade with PD98059, a specific inhibitor of MAPK kinase 1, prevented the expression of the alpha2 integrin subunit in cells induced to become megakaryocytic. We provide a model of megakaryocytic differentiation in which expression of the alpha2 integrin gene requires signaling via the MAP kinase pathway to activate two tandem AP1 binding sites in the alpha2 integrin enhancer.  (+info)

Activation of stimulus-specific serine esterases (proteases) in the initiation of platelet secretion. I. Demonstration with organophosphorus inhibitors. (6/11030)

The effect of organophosphorus inhibitors of serine esterases (proteases) on secretion from washed rabbit platelets was examined. Five noncytotoxic stimuli were employed: collagen, thrombin, heterologous anti-platelet antibody (in the absence of complement), rabbit C3 bound to zymosan, and platelet activating factor derived from antigen-stimulated, IgE-sensitized rabbit basophils. Diisoprophyl phosphofluoridate, three series of p-nitrophenyl ethyl phosphonates, and a series of cyclohexyl phenylalkylphosphonofluridates were all found to be inhibitory to the platelet secretion. These are irreversible inhibitors of serine proteases but in this system were only inhibitory if added to the platelets concurrently with the stimuli. Pretreatment of either the platelets or the stimuli with the inhibitors followed by washing, was without effect on the subsequent reaction. This suggested the involvement of stimulus-activatable serine proteases in the secretory process. The concept was supported by finding that nonphosphorylating phosphonates or hydrolyzed phosphonates or phosphonofluoridates were without inhibitory action. The effect of a series of phosphonates or phosphonoflouridates in inhibiting each stimulus exhibited a unique activity-structure profile. The demonstration of such unique profiles with four series of inhibitors for each of the five stimuli was interpreted as demonstrating that a specific activatable serine protease was involved in the platelet secretory response to each stimulus.  (+info)

Glycoprotein (GP) Ib-IX-transfected cells roll on a von Willebrand factor matrix under flow. Importance of the GPib/actin-binding protein (ABP-280) interaction in maintaining adhesion under high shear. (7/11030)

Adhesion of platelets to sites of vascular injury is critical for hemostasis and thrombosis and is dependent on the binding of the vascular adhesive protein von Willebrand factor (vWf) to the glycoprotein (GP) Ib-V-IX complex on the platelet surface. A unique but poorly defined characteristic of this receptor/ligand interaction is its ability to support platelet adhesion under conditions of high shear stress. To examine the structural domains of the GPIb-V-IX complex involved in mediating cell adhesion under flow, we have expressed partial (GPIb-IX), complete (GPIb-V-IX), and mutant (GPIbalpha cytoplasmic tail mutants) receptor complexes on the surface of Chinese hamster ovary (CHO) cells and examined their ability to adhere to a vWf matrix in flow-based adhesion assays. Our studies demonstrate that the partial receptor complex (GPIb-IX) supports CHO cell tethering and rolling on a bovine or human vWf matrix under flow. The adhesion was specifically inhibited by an anti-GPIbalpha blocking antibody (AK2) and was not observed with CHO cells expressing GPIbbeta and GPIX alone. The velocity of rolling was dependent on the level of shear stress, receptor density, and matrix concentration and was not altered by the presence of GPV. In contrast to selectins, which mediate cell rolling under conditions of low shear (20-200 s-1), GPIb-IX was able to support cell rolling at both venous (150 s-1) and arterial (1500-10,500 s-1) shear rates. Studies with a mutant GPIbalpha receptor subunit lacking the binding domain for actin-binding protein demonstrated that the association of the receptor complex with the membrane skeleton is not essential for cell tethering or rolling under low shear conditions, but is critical for maintaining adhesion at high shear rates (3000-6000 s-1). These studies demonstrate that the GPIb-IX complex is sufficient to mediate cell rolling on a vWf matrix at both venous and arterial levels of shear independent of other platelet adhesion receptors. Furthermore, our results suggest that the association between GPIbalpha and actin-binding protein plays an important role in enabling cells to remain tethered to a vWf matrix under conditions of high shear stress.  (+info)

Activation of G12/G13 results in shape change and Rho/Rho-kinase-mediated myosin light chain phosphorylation in mouse platelets. (8/11030)

Platelets respond to various stimuli with rapid changes in shape followed by aggregation and secretion of their granule contents. Platelets lacking the alpha-subunit of the heterotrimeric G protein Gq do not aggregate and degranulate but still undergo shape change after activation through thromboxane-A2 (TXA2) or thrombin receptors. In contrast to thrombin, the TXA2 mimetic U46619 led to the selective activation of G12 and G13 in Galphaq-deficient platelets indicating that these G proteins mediate TXA2 receptor-induced shape change. TXA2 receptor-mediated activation of G12/G13 resulted in tyrosine phosphorylation of pp72(syk) and stimulation of pp60(c-src) as well as in phosphorylation of myosin light chain (MLC) in Galphaq-deficient platelets. Both MLC phosphorylation and shape change induced through G12/G13 in the absence of Galphaq were inhibited by the C3 exoenzyme from Clostridium botulinum, by the Rho-kinase inhibitor Y-27632 and by cAMP-analogue Sp-5,6-DCl-cBIMPS. These data indicate that G12/G13 couple receptors to tyrosine kinases as well as to the Rho/Rho-kinase-mediated regulation of MLC phosphorylation. We provide evidence that G12/G13-mediated Rho/Rho-kinase-dependent regulation of MLC phosphorylation participates in receptor-induced platelet shape change.  (+info)

Circulating platelets consist of subpopulations with different age, maturation state and size. In this review, we address the association between platelet size and platelet function and summarize the current knowledge on platelet subpopulations including reticulated platelets, procoagulant platelets and platelets exposing signals to mediate their clearance. Thereby, we emphasize the impact of platelet turnover as an important condition for platelet production in vivo. Understanding of the features that characterize platelet subpopulations is very relevant for the methods of platelet concentrate production, which may enrich or deplete particular platelet subpopulations. Moreover, the concept of platelet size being associated with platelet function may be attractive for transfusion medicine as it holds the perspective to separate platelet subpopulations with specific functional capabilities.
OBJECTIVE-The goal of this study was to specifically estimate the effectiveness of platelet releasate, a widely available treatment administered by a proprietary group of wound care centers (WCCs) for the treatment of diabetic neuropathic foot ulceration.. RESEARCH DESIGN AND METHODS-Treatment effectiveness was estimated in a retrospective cohort study controlling for treatment selection bias using logistic regression-derived propensity scores.. RESULTS-Platelet releasate was more effective than standard care. The relative risk for a wound to heal after treatment with platelet releasate compared with standard care at a WCC varied from 1.14 (95% CI 1.03-1.27) to 1.59 (1.49-1.70). The effect was greatest in those with the most severe wounds, i.e., large wounds that affect deeper anatomical structures.. CONCLUSIONS-Within the limitations of the ability of propensity score analysis to control for selection bias, platelet releasate is more effective than standard therapy. This effect is more ...
In this study, we show that the platelet surface expression of glycoprotein (GP) V is regulated by two independent mechanisms. While confirming that both thrombin and neutrophil elastase proteolyse GPV, we show that neutrophil cathepsin G, thrombin receptor activating peptide (TRAP), and a combination of ADP and epinephrine can each result in a decrease in the platelet surface expression of GPV by a nonproteolytic mechanism: a cytoskeletal-mediated redistribution of platelet surface GPV to the surface-connected canalicular system (SCCS). Four independent lines of evidence documented the nonproteolytic nature of this decrease in the platelet surface expression of GPV. First, flow cytometric studies showed that cathepsin G, TRAP, and ADP/epinephrine decreased the platelet surface expression of GPV without changing the total platelet content of GPV. Second, immunoelectron microscopy directly demonstrated translocation of GPV from the platelet surface to the SCCS. Third, the cathepsin G-, TRAP-, and ADP
TY - JOUR. T1 - Relationship between high platelet turnover and platelet function in high-risk patients with coronary artery disease on dual antiplatelet therapy. AU - Cesari, Francesca. AU - Marcucci, Rossella. AU - Caporale, Roberto. AU - Paniccia, Rita. AU - Romano, Eloisa. AU - Gensini, Gian Franco. AU - Abbate, Rosanna. AU - Gori, Anna Maria. PY - 2008/5. Y1 - 2008/5. N2 - A high platelet turnover rate produce a population of immature reticulated platelets (RP) that could confer, despite of antiplatelet drugs, a residual platelet reactivity (RPR) in coronary artery disease (CAD) patients. To assess the influence of RP on platelet reactivity in CAD patients on dual antiplatelet therapy we measured RP in 372 patients by using the Sysmex XE-2100 haematology analyzer and platelet function by optical platelet aggregometry (PA) on platelet-rich-plasma induced by 1 mmol arachidonic acid (AA-PA) and 10 μM ADP (ADP-PA). RPR was defined as either AA-PA ,20% or ADP-PA ,70%. RP were expressed as a ...
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TY - JOUR. T1 - Influence of rheologic changes and platelet-neutrophil interactions on cell filtration in sepsis. AU - Kirschenbaum, Linda A.. AU - Aziz, Mohammed. AU - Astiz, Mark E.. AU - Saha, Dhanonjoy C.. AU - Rackow, Eric C.. PY - 2000/1/1. Y1 - 2000/1/1. N2 - We examined the role of erythrocyte (red blood cell; RBC) aggregation and deformability, neutrophil (polymorphonuclear neutrophil; PMN) deformability, whole-blood viscosity, and platelet-neutrophil interactions on cell filtration in subjects who were critically ill with sepsis (CIS), critically ill noninfected subjects (CINS), and healthy controls (C). We assessed cell deformability by filtration through filters of 5-μm pore size. Whole blood, RBC, PMN, and combinations of PMN and RBC were studied. Viscometry was done on isolated RBC. Platelet-PMN interactions were assessed with monoclonal antibodies to CD41 and activated CD53 platelet receptors, and to CD66b PMN receptors. Filtration pressure (Pi) for CIS was significantly greater ...
Platelet membrane receptor P2Y12 H1/H2 polymorphisms is highly associated with cerebral infarction: a case-control study Shu-Jun Lu, Xiao-Sheng Zhou, Qi Zheng, Hong-Liang Chen, Yan-Lei Geng Department of Neurology, Binzhou Medical University Hospital, Binzhou, Peoples Republic of China Objectives: This study aimed to determine the relationship between the polymorphisms of the H1/H2 gene of platelet membrane receptor P2Y12 and cerebral infarction (CI) in a Han population in North Shandong Province, Peoples Republic of China. Patients and methods: A case-control study, which involved 168 nonstoke subjects (contrast group) and 152 CI patients (CI group), was conducted. The state of subjects in the CI group was validated by computed tomography or MRI. The clinical data were categorized into two groups. The data included age, gender, smoking, drinking, shrinkage pressure, diastolic blood pressure, blood glucose, cholesterol, triglyceride, low-density lipoprotein, high-density lipoprotein, serum uric
Partial purification and characterization of serine protease activity in platelets and platelet releasates from patients with Quebec platelet disorder Conference Paper ...
Approach and Results-To model standard antiplatelet therapy, platelets were treated in vitro with aspirin, the P2Y12 receptor blocker prasugrel active metabolite, and aspirin plus prasugrel active metabolite. Different proportions of uninhibited platelets were then introduced. Light transmission aggregometry analysis demonstrated clear positive associations between proportions of drug-free platelets and percentage platelet aggregation in response to a range of platelet agonists. Using differential platelet labeling coupled with advanced flow cytometry and confocal imaging, we found aggregates formed in mixtures of aspirin-inhibited platelets and drug-free platelets were characterized by intermingled platelet populations. This distribution is in accordance with the ability of drug-free platelets to generate thromboxane A2 and so drive secondary platelet activation. Conversely, aggregates formed in mixtures of prasugrel active metabolite-inhibited or aspirin plus prasugrel active ...
Introduction: S100A1 is a member of the S100 family of calcium-binding proteins. S100A1 controls Ca2+ dynamics in cardiomyocytes and plays an important role in heart failure. S100A1 is also strongly expressed in mouse platelets, but its role in platelet biology has not been investigated.. Goal: To determine the role of S100A1 in platelet activation and thrombosis.. Methods and Results: Platelet activation in response to threshold levels of convulxin, a specific agonist for the collagen receptor GPVI, showed significantly increased activation of αIIbβ3 integrin and α-granule release in S100A1-deficient (SKO) platelets compared with wild-type (WT) platelets. Consistently, SKO platelets also showed a more robust aggregation response to convulxin and collagen. In contrast, SKO platelets responded normally to stimulation with PAR4 receptor-activating peptide or ADP. Adhesion of SKO platelets to collagen under flow conditions was not significantly different to that of WT platelets. However, we ...
Oxidized LDL and platelets play a central role in the pathogenesis of atherosclerosis and ischemic cardiovascular diseases. Lysophosphatidic acid (LPA) is a thrombogenic substance that accumulates in mildly-oxidized LDL and in human atherosclerotic lesions, and is responsible for the initial platelet activation, shape change, induced by mildly-oxidized LDL and extracts of lipid-rich atherosclerotic plaques (Siess et al., 1999 Proc Natl Acad Sci USA 1999). LPA directly induced platelet shape change in blood and platelet-rich plasma (PRP) obtained from all blood donors. Albumin was one of the main inhibiting factors of platelet shape change in plasma. Interestingly LPA, at concentrations slightly above plasma levels, induced platelet shape change and aggregation in blood. 1-alkyl-LPA (16:0) was almost 20-fold more potent than 1-acyl-LPA (16:0). LPA-stimulated platelet aggregation in blood and PRP was donor-dependent. LPA-induced aggregation in blood could be completely blocked by the ADP- ...
A method for determining platelet activation by utilizing numeric counts of platelets before a sample of platelets has been activated and after the activatable platelets are activated with a platelet activation agonist and using the difference between such counts as an, indication of the platelet activity of the sample. There is also disclosed a method for using the electronic impedance cell counting technique for determining platelet activation wherein EDTA is used as a preservative by counting the platelets in an EDTA preserved sample using an electronic impedance cell counting technique and subtracting from that number the number of platelets remaining after the activatable platelets in a second sample have been activated with a platelet agonist in the absence of EDTA and using that difference as a measure of platelet activity.
Most heart attacks and strokes are caused by blood clots (thrombi) that block the vasculature. Because disease-causing arterial thrombosis depends on blood platelets, platelet inhibitors such as aspirin and clopidogrel effectively decrease the risk of thrombosis; however, they also impair platelet-dependent hemostasis that staunches bleeding from wounds and can therefore produce excessive bleeding. Experimental studies show that a reduction in the number of platelets also inhibits thrombosis, but these treatments also interfere with platelet function. Because normal hemostasis requires that the platelet concentration remain within a physiological range in the circulation, we evaluated whether lowering the number of circulating platelets-but only to a value still within the normal range-by inhibiting platelet formation in the bone marrow inhibits acute thrombogenesis in baboons. We reduced the platelet count with an inhibitor against the megakaryocyte-promoting hormone thrombopoietin and then ...
Platelets play a key and beneficial role for primary hemostasis on the disruption of the integrity of vessel wall. Platelet adhesion and activation at sites of vascular wall injury is initiated by adhesion to adhesive macromolecules, such as collagen and von Willebrand factor (vWF), or by soluble platelet agonists, such as ADP, thrombin, and thromboxane A2. Different receptors are stimulated by various agonists, almost converging in increasing intracellular Ca2+ concentration that stimulate platelet shape change and granule secretion and ultimately induce the inside-out signaling process leading to activation of the ligand-binding function of integrin alpha IIb beta 3. Binding of alpha IIb beta 3 to its ligands, mainly fibrinogen, mediates platelet adhesion and aggregation and triggers outside-in signaling, resulting in platelet spreading, additional granule secretion, stabilization of platelet adhesion and aggregation, and clot retraction ...
human platelet receptor for type III collagen: MW 68-72 kDa; platelet receptor involved in platelet interaction with type III collagen, localized within platelet lipid rafts where it could interact with other platelet receptors for collagen (GP VI and alpha2beta1 integrin) for efficient platelet activation
Reuters) - Scientists have for the first time created blood platelet cells by reprogramming stem cells derived from adult cells, offering the potential for a renewable supply of the fragile blood component.. Researchers at the Center for iPS Cell Research and Application at Kyoto University in Japan presented data here at a meeting of the American Society of Hematology showing they were able to create the cells in the laboratory and confirm they had the same life span as normal human platelets when infused in mice.. The next step will be to conduct a trial to determine whether our platelets can function in the human body and potentially provide a stable supply of platelets at a predefined quality and quantity that can then be used for transfusion therapy, D. Koji Eto, professor at the Kyoto center and senior author of the study, said in a statement.. Induced pluripotent stem cells, or iPS cells, are produced by manipulating ordinary human skin or blood cells back to a state in which they are ...
inbook{d2d8fceb-9b60-4916-ad95-3b1f6f9113f7, abstract = {,p,Many pathogenic bacteria have been reported to interact with human platelets to mediate platelet activation and aggregation. The importance of these interactions to the immune response or pathogenesis of bacterial infection has not been clarified. It may therefore be valuable to assess platelet responses mediated by diverse strains of bacteria. Here, I describe a method to study platelet integrin activation and granule release using flow cytometry, and a complementary method to study platelet aggregation using a dedicated platelet aggregometer. The combination of these methods represents a rapid and cost-effective strategy to provide mechanistic insight on the type of platelet response mediated by the bacteria.,/p,}, author = {Shannon, Oonagh}, issn = {10643745}, keyword = {Bacteria,Coagulation,Flow cytometry,Platelets,Streptococci}, language = {eng}, pages = {267--273}, publisher = {Humana Press}, series = {Methods in Molecular ...
The present study demonstrates that in type 2 DM patients with CAD receiving maintenance aspirin and clopidogrel therapy, the presence of moderate/severe CKD is associated with higher degrees of platelet reactivity compared with patients with normal renal function/mild CKD. In particular, after adjustment for potential confounders, patients with creatinine clearance ,60 ml/min had an almost 4-fold increase in the likelihood of showing high platelet reactivity after ADP stimuli and over a 2-fold increase in high platelet reactivity after collagen stimuli. Importantly, these patients with high platelet aggregability also have increased markers of platelet activation. Overall, these findings are indicative not only of dysfunctional purinergic signaling mediated ADP receptors but also of the presence of a hyper-reactive platelet phenotype with upregulation of multiple signaling pathways. Therefore, these pharmacodynamic observations might explain the elevated prevalence of ischemic complications, ...
OBJECTIVE-: Protease-activated receptor 4 (PAR4) is a key regulator of platelet reactivity and is encoded by F2RL3, which has abundant rare missense variants. We aimed to provide proof of principle that rare F2LR3 variants potentially affect on platelet reactivity and responsiveness to PAR1 antagonist drugs and to explore underlying molecular mechanisms. APPROACH AND RESULTS-: We identified 6 rare F2RL3 missense variants in 236 cardiac patients, of which the variant causing a tyrosine 157 to cysteine substitution (Y157C) was predicted computationally to affect most on PAR4 structure. Y157C platelets from 3 cases showed reduced responses to PAR4-activating peptide and to α-thrombin compared with controls, but no reduction in responses to PAR1-activating peptide. Pretreatment with the PAR1 antagonist vorapaxar caused lower residual α-thrombin responses in Y157C platelets than in controls, indicating greater platelet inhibition. HEK293 cells transfected with a PAR4 Y157C expression construct had ...
TY - JOUR. T1 - Four types of human platelet lysate, including one virally inactivated by solvent-detergent, can be used to propagate Wharton jelly mesenchymal stromal cells. AU - Chen, Ming Sheng. AU - Wang, Tsung Jen. AU - Lin, Hsiu Chen. AU - Thierry, Burnouf. PY - 2019/3/25. Y1 - 2019/3/25. N2 - There is accumulating experimental evidence that human platelet lysate (HPL) made from platelet concentrates can replace fetal bovine serum (FBS) as a xeno-free clinical-grade supplement of growth media to expand mesenchymal stromal cells (MSCs). However, uncertainties exist in regard to impacts that various manufacturing methods of HPL can exert on the expansion and differentiation capacity of MSCs. In particular, there is a need to evaluate the possibility of implementing virus-inactivation treatment during HPL production to ensure optimal safety of industrial HPL pools. Expired human platelet concentrates from four different donors were pooled and subjected to freeze-thaw cycles (-80/+37 °C), ...
The typical reconstruction model of an unstimulated human platelet is presented. It shows a strict spatial order of organelles and enodmembrane systems. The surface-connected vesicle system is predominantly found in the periphery. The dense tubules constitute a continuous, interconnected system found just under the surface of the platelet. They are particularly pronounced in the vicinity of the marginal microtubules. All organelles are in contact with the dense tubular system (DTS). Granules, vesicles and plasmalemma fuse into the tubules. The platelet organelles are centrally located. Lysosomal granules are primarily shperical and all of them contain a nucleoid. This speaks in favor of a single type of storage organelle in the platelet. The dense bodies and their remnants (large, solitary vesicles) are not joined by the other endomembrane systems, as in the case of the granules. A particular pathways for the release of substances from these organelles is now morphologically plausible. Mitochondria are
TY - JOUR. T1 - Platelet function after cardiac surgery and its association with severe postoperative bleeding. T2 - the PLATFORM study. AU - for The Surgical and Clinical Outcome Research (SCORE) Group. AU - Ranucci, Marco. AU - Pistuddi, Valeria. AU - Di Dedda, Umberto. AU - Menicanti, Lorenzo. AU - De Vincentiis, Carlo. AU - Baryshnikova, Ekaterina. PY - 2018/1/1. Y1 - 2018/1/1. N2 - Platelet dysfunction after cardiac surgery is a determinant of postoperative bleeding. The existing guidelines suggest the use of desmopressin and/or platelet concentrate transfusions in case of platelet dysfunction in bleeding patients, but no cut-off values for platelet activity exist in the literature. The Platelet Function in the Operating Room (PLATFORM) study aims to identify the relationship between platelet function after cardiopulmonary bypass and severe bleeding, finding adequate predictive values of platelet function for severe bleeding. The PLATFORM is a prospective cohort study on 490 adult patients ...
The frequency and severity of bacteremic infections has increased over the last decade and bacterial endovascular infections (i.e., sepsis or endocarditis) are associated with high morbidity and mortality. Bacteria or secreted bacterial products modulate platelet function and, as a result, affect platelet accumulation at sites of vascular infection and inflammation. However, whether bacterial products regulate synthetic events in platelets is not known. In the present study, we determined if prolonged contact with staphylococcal α-toxin signals platelets to synthesize B-cell lymphoma (Bcl-3), a protein that regulates clot retraction in murine and human platelets. We show that α-toxin induced αIIbβ3-dependent aggregation (EC50 2.98 µg/mL ± 0.64 µg/mL) and, over time, significantly altered platelet morphology and stimulated de novo accumulation of Bcl-3 protein in platelets. Adherence to collagen or fibrinogen also increased the expression of Bcl-3 protein by platelets. α-toxin altered Bcl-3
Methods were developed for measuring changes in platelet sensitivity to a release-inducing stimulus and in platelet cyclic AMP in fresh whole blood samples from rabbits. These techniques permitted detection of the effects of exogenous and endogenous prostacyclin on circulating platelets. In these methods, rabbit platelets were labeled in vitro by incubation with [14C]serotonin and [3H]adenine and then transfused into other rabbits. Release of platelet [14C]serotonin by a standard dose of synthetic platelet-activating factor (40 pmol/ml) and the platelet cyclic [3H]AMP levels were then measured in citrated blood from the conscious animals within 2 min of arterial puncture. Bolus intravenous injections of prostacyclin (1-10 nmol/kg) caused concentration-dependent increases in platelet cyclic AMP after 2 min, which decreased approximately 75% by 5 min, and disappeared after 30 min. Significant inhibition of the platelet release reaction was detected 2 min but not 5 min after injection of 10 nmol of ...
TY - JOUR. T1 - Transfection of human platelets with short interfering RNA. AU - Hong, Wei. AU - Kondkar, Altaf A.. AU - Nagalla, Srikanth. AU - Bergmeier, Wolfgang. AU - Jin, Ying. AU - Herman, Jay H.. AU - Bray, Paul F.. PY - 2011/6. Y1 - 2011/6. N2 - Platelets contain mRNAs and are capable of translating mRNA into protein, and it has been previously demonstrated that platelets increase their levels of integrin β3 overtime while in blood bank storage conditions. We are unaware of prior attempts to introduce nucleic acids into platelets. Considering the potential clinical and research utility of manipulating platelet gene expression, we tested whether small interfering RNAs (siRNAs) could be transfected into normal human platelets. Multiple conditions were tested, including lipofectamine versus electroporation, different amounts of siRNA, the effect of different buffers and the presence of plasma during transfection, and the time for optimal siRNA incorporation after transfection. Using flow ...
Release: Dec. 3, 1999. UI participates in multi-center study testing new platelet sterilizing strategy. IOWA CITY, Iowa The University of Iowa Hospitals and Clinics is participating in a multi-center trial testing the efficacy and safety of a new way to cleanse infectious organisms from donated platelets before the blood product is administered to patients.. The UI department of pathology has received a one-and-half-year grant from health care companies Baxter and Cerus for the study titled Determination of the therapeutic efficacy and safety of photochemically treated platelets in thrombocytopenic patients.. Thrombocytopenia is a condition characterized by low blood platelet counts. Platelets are the clotting particles in blood.. The new photochemical strategy attempts to sterilize donated platelets against bacteria and viruses, which may be present in blood products. The goal of the study is to establish that the platelets are not damaged from the sterilization process. Preliminary data ...
The TEMPLATE study design will enable unbiased comparison of the effects of TIC versus TIC + ASP on platelet activity in patients with coronary artery disease. It will also enable a longitudinal comparison of the effects TIC and TIC + ASP with the effects of ASP alone in the same patients. The laboratory tests selected for this study will enable measurement of functional platelet responses to a panel of activating agonists using LTA, flow cytometry and flow chamber tests, selected to measure the extent of inhibition of the multiple platelet activation pathways. We will also measure the extent of baseline platelet activity by testing unstimulated platelets by flow cytometry and with the soluble platelet activation biomarker tests. Together, these data will provide a comprehensive description of the overall pharmacodynamic effects of the different antiplatelet treatments. This information has not been reported previously in cohorts of patients with coronary artery disease receiving TIC or TIC + ...
Blood platelets commonly called megakaryocytes are one of the three tiny cellular components of the blood which helps in the stoppage of bleeding.. Other cellular components of blood are the red blood cells and white blood cells. All the cellular components bathe in the plasma.. Nearly half a billion blood platelets are being formed in the bone marrow every day. These play a primary role in the formation of blood clot. Here I would like to define the two common terms related to the platelet plug formation when the blood vessel in injured.. * Adhesion means sticking of blood platelets with the wall of the bleeding vessel.. * Aggregation means sticking of the platelets with each other.. Normally, in the circulating blood, platelets keep on flowing without any adhesion or aggregation. However, injury to the wall of blood vessel is the point when blood platelets start clinging to the injured part of the vessel wall. At the same time the platelets aggregate with one another and completely seal the ...
The normal circulating platelet count is maintained within relatively narrow limits (150,000-450,000 platelets/μL in Northern Europeans and 90,000-300,000 platelets/μL in people of Mediterranean descent). This difference is related to an inherited slight variation in individual platelet volume (size). The platelet volume is inversely related to the platelet count, so the mass of circulating platelets is the same for these 2 populations. Approximately one-third of platelets are sequestered in the spleen at any one time. Splenic sequestration of platelets can increase dramatically with splenomegaly. Since a platelet has a lifespan of approximately 9-10 days, some 15,000-45,000 platelets/μL must be produced each day to maintain a steady state. New platelet production is the responsibility of the megakaryocyte, a very large multinucleated cell (10,750 fL) found in relatively small numbers in the marrow (0.1% of marrow cells) (Figure 31-1). As with other hematopoietic cells, megakaryocytes are ...
Research in the Laboratory for Hemostasis and Platelet Biology, led by Dr. Andrew Johnson, focuses on understanding genetic and genomic underpinnings of this individual variability in therapeutically targeted CVD pathways ...
Although tumor dormancy is highly prevalent, the underling mechanisms are still mostly unknown. It is unclear which lesions will progress and become a disseminated cancer, and which will remain dormant and asymptomatic. Yet, an improved ability to predict progression would open the possibility of timely treatment and improvement in outcomes. We have recently described the ability of platelets to selectively uptake angiogenesis regulators very early in tumor growth, and proposed their use as an early marker of malignancy. In this review we will summarize current knowledge about these processes and will discuss the possibility of using platelet content to predict presence of occult tumors.
OBJECTIVES: We sought to examine whether patients with stable coronary artery disease (CAD) have increased platelet reactivity and an enhanced propensity to form monocyte-platelet aggregates. BACKGROUND: Platelet-dependent thrombosis and leukocyte infiltration into the vessel wall are characteristic cellular events seen in atherosclerosis. METHODS: Anticoagulated peripheral venous blood from 19 patients with stable CAD and 19 normal control subjects was incubated with or without various platelet agonists and analyzed by whole blood flow cytometry. RESULTS: Circulating degranulated platelets were increased in patients with CAD compared with control subjects (mean [+/- SEM] percent P-selectin-positive platelets: 2.1 +/- 0.2 vs. 1.5 +/- 0.2, p andlt; 0.01) and were more reactive to stimulation with 1 micromol/liter of adenosine diphosphate (ADP) (28.7 +/- 3.9 vs. 16.1 +/- 2.2, p andlt; 0.01), 1 micromol/liter of ADP/epinephrine (51.4 +/- 4.6 vs. 37.5 +/- 3.8, p andlt; 0.05) or 5 micromol/liter of thrombin
Our data clearly show that human platelets can both bind and degrade full-length rhTPO. The affinity constants derived from the binding data indicate that rhTPOs affinity for platelet receptors was similar to that for the cloned c-Mpl receptor construct, gD-Mpl. The binding constants were also similar for rhTPO binding to PRP at 37°C and WP at 22°C. These data show that platelets bind rhTPO, likely via c-Mpl with high affinity (∼350 pmol/L), and that this binding is saturable. Scatchard analysis predicted a low number of (∼23 to 224) binding sites per platelet. However, this estimate may be low, because c-Mpl binding sites on circulating platelets are likely partially occupied by endogenous circulating TPO.9,10 If we assume the number of available c-Mpl receptors is ∼25 to 200 per platelet and that the approximate number of platelets per liter of blood in humans is 200 × 109, then the predicted binding capacity would be ∼8 to 64 pmol per liter of blood. This approximation suggests ...
Subjects for all study groups will be male and between the ages of 25 and 65. Hypertension & Depression Group: Hypertension controlled with an ACE-inhibitor anti-hypertensive; no co-morbid medical conditions known to influence psychological functioning or platelet calcium responses including uncontrolled diabetes, MI or CVA within 6 months of enrollment, secondary hypertension; depression as diagnosed by structured interview and HDRS score of 18; no active participation in another clinical trial; no current suicidal/ homicidal ideation. Hypertension Group: Hypertension controlled with an ACE-inhibitor anti-hypertensive; no co-morbid medical conditions known to influence psychological functioning or platelet calcium responses including uncontrolled diabetes, MI or CVA within 6 months of enrollment, secondary hypertension; no active participation in another clinical trial; no current suicidal/ homicidal ideation. Depression Group: No co-morbid medical conditions known to influence psychological ...
Platelets may interact directly with their targets and perform its killing function. For example, platelets may bind and wrap bacteria (Youssefian et al., 2002) or induce their aggregation (OBrien et al., 2002), leading to degranulation. During malaria infection, platelets have also been described to perform the direct killing of plasmodium parasites in their blood stage forms in a PF4-dependent manner (McMorran et al., 2009, 2012), leading to the general perception that platelets play protective roles during an infection. However, a recent in vivo study in mice paradoxically found that platelet depletion did not lead to higher parasitemia levels (Gramaglia et al., 2017). Instead, links were found between the presence of platelets and malarial pathogenesis via CD40 interactions. Because about two thirds (Jadhav et al., 2004) of malarial infections are accompanied by thrombocytopenia, it thus remains a quandary for clinicians to decide if they should be boosting or inhibiting platelet function ...
1. A new fixing solution is described, which preserves the platelets and prevents contact hemolysis of the erythrocytes, so that counts of both corpuscles may be made in the same preparation.. 2. Comparative counts of platelets in arteries and veins show that arterial blood contains a larger number of platelets than venous blood. This difference is accentuated under experimental conditions that cause a reduction in the number of platelets. It is concluded that new platelets are added to the blood in the capillary areas of the lungs, and that there is a corresponding destruction of platelets as the blood passes through the capillary areas of the systemic circulation.. 3. Perfusion of the lungs with a platelet-preserving solution, compared with that of other organs, gives evidence of the existence of a source of platelet material in the lungs.. 4. Histological examination of the lungs with a technique adequate to give a differential staining of platelet material demonstrates the presence of giant ...
New studies in mice suggest that blood platelets can destroy deadly malaria parasites, but a single dose of aspirin may be enough to thwart their killing power., , , , The findings could have important im...
ANTICOAGULANTS AND THROMBOLYTIC DRUGSHaemostasisVascular injury results firstly in vasoconstriction and formation of platelet plug at the site of injury (primary haemostasis). The platelet plug is then stabilized by the formation of a fibrin meshwork, resulting from activation of the coagulation cascade.Fibrin is eventually cleared through digestion by fibrinolytic enzymes.Primary HaemostasisWhen endothelial integrity is breached, platelets adhere to exposed subendothelial collagen. The adherent platelets become activated result in;1) Exposure of fibrinogen receptors, allowing fibrinogen to bind and cross-link adjacent platelets. The process is known as platelet aggregation. The platelet fibrinogen receptor consists of a complex of glycoproteins IIb and IIIa on the platelet membrane.2) Release of contents of secretory granules including substances such as adenosine diphosphate (ADP) which promote further platelet activation.3) Synthesis of thromboxane A2 which also acts to promote further ...
AimsTo assess whether platelet reactivity is increased in offspring of patients with early acute myocardial infarction (AMI) and its possible relation with endothelial dysfunction.Methods and resultsWe studied 23 healthy children (15 ± 3 years, 13 males) of patients with early AMI (≤50 years old; Group 1) and 21 healthy children of healthy subjects without any history of cardiovascular disease (14 ± 3 years, 10 males; Group 2). Platelet reactivity was assessed by flow cytometry as the increase in monocyte-platelet aggregates (MPA) and CD41 and PAC-1 platelet expression in response to exercise stress test (EST), adenosine diphosphate (ADP) stimulation (10 -7 M), or both. Endothelial function was assessed by measuring brachial artery dilation during post-ischaemic forearm hyperaemia [flow-mediated dilation (FMD)]. Both EST and ADP induced a higher percentage increase in platelet receptor expression in Group 1, compared with Group 2, with the most significant difference being shown for the ...
As conventional tissue biopsies have several drawbacks, much effort has been directed toward the development of minimal-invasive liquid biopsy platforms for detecting and profiling cancer.. Platelets are the second most abundant cells in blood and have very versatile functions both in physiological and pathophysiological conditions. When exposed to tumors and their environment, platelets exchange biomolecules with tumor cells changing the platelets RNA profile, resulting in tumor-mediated education of the platelets. Our research group and collaborators have previously shown that platelets sequester material while in circulation and with that ability accumulate cancer specific information. Platelet RNA profiles or detection of tumor-derived biomarkers within them may provide insight into ongoing cancer-related processes in a patient, allowing for implementation of personalized therapy strategies.. This thesis evaluates whether circulating platelets could have a potential role (as a liquid biopsy ...
Platelets, also called thrombocytes (thromb- + -cyte, blood clot cell), are a component of blood whose function (along with the coagulation factors) is to stop bleeding by clumping and clotting blood vessel injuries. Platelets have no cell nucleus: they are fragments of cytoplasm that are derived from the megakaryocytes of the bone marrow, and then enter the circulation. These unactivated platelets are biconvex discoid (lens-shaped) structures, 2-3 µm in greatest diameter. Platelets are found only in mammals, whereas in other animals (e.g. birds, amphibians) thrombocytes circulate as intact mononuclear cells. On a stained blood smear, platelets appear as dark purple spots, about 20% the diameter of red blood cells. The smear is used to examine platelets for size, shape, qualitative number, and clumping. The ratio of platelets to red blood cells in a healthy adult is 1:10 to 1:20. The main function of platelets is to contribute to hemostasis: the process of stopping bleeding at the site of ...
After deposition of a platelet monolayer over the exposed VWF and collagen, the next step required for thrombus formation is the recruitment of additional platelets from the flowing blood, which upon activation acquire the ability to stick to each other in a process commonly referred to as platelet aggregation. This is made possible by the local accumulation of soluble agonists that are secreted/produced by adherent-activated platelets, including ADP, TxA2, epinephrine and thrombin. The final step is activation of αIIbβ3, causing a conformational change that enables it to bind fibrinogen and VWF, allowing stable bridges between platelets. The great number of αIIbβ3 copies on the platelet surface, (40,000-80,000), allows the assembly of large aggregates at the site of vascular injury. Activation of αIIbβ3 integrin requires agonist-driven activation events in recruited platelets, referred to as inside-out signaling, including the sequential activation of one or more PLC isoforms yielding a ...
Fig.10 Design of CREKA-Lipo-T nanoparticles and their proposed antimetastatic mechanism within tumor tissues. (A) Proposed mechanism of action of CREKA-Lipo-T nanoparticles. Normally, tumor growth factor (TGF)-b secreted by platelets induces transition of tumor cells to a mesenchymal-like phenotype (I). Platelets can also protect tumor cells against attack from natural killer (NK) cells (II). At distant sites, platelets assist metastatic cells to cross the local endothelium by secreting numerous cytokines. Following treatment, CREKA-Lipo-T actively targets microthrombi in tumor vessel walls and releases ticagrelor slowly and locally. Ticagrelor binds to tumor-associated platelets and inhibits their functions. The release of TGF-b from platelets and the interaction between platelets and tumor cells are abolished, leading to decreased epithelial-mesenchymal-like transition of tumor cells and thus inhibiting their invasion capacity. When tumor cells are present in circulation, compromised platelets ...
TY - JOUR. T1 - Preferential binding of platelets to monocytes over neutrophils under flow. AU - Ahn, Kyung C.. AU - Jun, Andy J.. AU - Pawar, Parag. AU - Jadhav, Sameer. AU - Napier, Susan. AU - McCarty, Owen J.T.. AU - Konstantopoulos, Konstantinos. PY - 2005/4/1. Y1 - 2005/4/1. N2 - This study was undertaken to systematically investigate the binding kinetics of platelet recruitment by monocytes relative to neutrophils in bulk suspensions subjected to shear as well as the molecular requirements of leukocyte-platelet binding. Hydrodynamic shear-induced collisions augment the proportion of monocytes with adherent platelets more drastically than that of neutrophils with bound platelets. These heterotypic interactions are further potentiated by platelet activation with thrombin or to a lesser extent by monocyte stimulation with N-formyl-methionyl-leucyl-phenylalanine (fMLP). Monocyte-platelet heteroaggregation increases with increasing shear rate and shear exposure time. Platelet P-selectin ...
Cooper, N., Heddle, N. M., de Haas, M., Reid, M. E., Lesser, M. L., Fleit, H. B., Woloski, B. M. R. and Bussel, J. B. (2004), Intravenous (IV) anti-D and IV immunoglobulin achieve acute platelet increases by different mechanisms: modulation of cytokine and platelet responses to IV anti-D by FcγRIIa and FcγRIIIa polymorphisms. British Journal of Haematology, 124: 511-518. doi: 10.1111/j.1365-2141.2004.04804.x ...
Tortuous blood vessels are often seen in humans in association with thrombosis, atherosclerosis, hypertension, and aging. Vessel tortuosity can cause high fluid shear stress, likely promoting thrombosis. However, the underlying physical mechanisms and microscale processes are poorly understood. Accordingly, the objectives of this study were to develop and use a new computational approach to determine the effects of venule tortuosity and fluid velocity on thrombus initiation. The transport, collision, shear-induced activation, and receptor-ligand adhesion of individual platelets in thrombus formation were simulated using discrete element method. The shear-induced activation model assumed that a platelet became activated if it experienced a shear stress above a relative critical shear stress or if it contacted an activated platelet. Venules of various levels of tortuosity were simulated for a mean flow velocity of 0.10 cm s−1 , and a tortuous arteriole was simulated for a mean velocity of 0.47 ...
Article see p 476. As megakaryocytes form platelets, they transfer STAT3 to proplatelet tips. Consequently, STAT3 is found in platelets that circulate in the bloodstream (Figure). The presence of STAT3 in platelets raises the question of whether it regulates functional responses in platelets or is simply a vestigial remnant of megakaryocytes. An argument for the leftover without function hypothesis is the anucleate status of platelets: simply stated, with no nucleus and no nuclear DNA there is no place for STAT3 to stick in platelets. The problem with this argument is that simple is no longer a common word used to describe platelets. Moreover, why would platelets expend energy to carry a protein that they do not need, especially since previous studies have shown that STAT3 undergoes signal-dependent phosphorylation in these anucleate cytoplasts?4 Well, any doubt regarding why STAT3 is present in platelets has been cleared up. Using a combination of pharmacological and genetic based tools, ...
Background: Coated-platelets, a subset of activated platelets observed with dual-agonist stimulation with collagen and thrombin, represent 30% of the platelet population in normal controls. In recently published work, we have shown that elevated coated-platelet levels (,45%) are predictive of stroke in asymptomatic carotid stenosis. We now investigate if platelet count and mean platelet volume (MPV) are related to coated-platelet levels.. Methods: Coated-platelet levels were measured in a cohort of asymptomatic outpatients referred for carotid ultrasound studies. Platelet count and mean platelet volume for each subject were recorded from the VA electronic medical record at the closest possible time period (within ≤6 months) to the date of coated-platelet sample. Correlations between each parameter and coated-platelet levels were determined and those reaching significance at p≤0.1 were included in a multiple regression model with LDL and systolic blood pressure (SBP), variables previously ...
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TY - JOUR. T1 - A study of whole blood platelet and white cell aggregation using a laser flow aggregometer. AU - Sun, J.. AU - Abel, E. W.. AU - Bancroft, A.. AU - McLaren, M.. AU - Belch, J. J. F.. PY - 2003. Y1 - 2003. N2 - Both platelet aggregation and white blood cell aggregation are involved in pathological processes such as thrombosis, atherosclerosis and chronic inflammation. People in older age groups are likely to suffer from cardiovascular diseases and may have increased white cell and platelet aggregation which could contribute to this increased risk. This study aimed to compare white cell and platelet aggregation between different age and gender groups. Whole blood white cell aggregation and platelet aggregation were carried out on healthy volunteers using cytometric techniques. It was found that both white cell and platelet aggregation in the elderly group (white cell aggregation median value, 0.08; range, 0.02-0.14; platelet aggregation median value, 0.32; range, 0.1-0.39) were ...
Systematic review (Open Access) on the comparison of different platelet transfusion thresholds prior to insertion of central lines in patients with thrombocytopenia from Cochrane Database of Systematic Reviews Comparison of different platelet transfusion thresholds prior to insertion of central lines in patients with thrombocytopenia #vascularaccess #FOAMva #FOAMed #FOAMcc #POCUS #patientsafety
Megakaryocytes generate platelets by remodeling their cytoplasm into long proplatelet extensions, which serve as assembly lines for platelet production. Although the mechanics of proplatelet elongation have been studied, the terminal steps of proplatelet maturation and platelet release remain poorly understood. To elucidate this process, released proplatelets were isolated, and their conversion into individual platelets was assessed. This enabled us to (a) define and quantify the different stages in platelet maturation, (b) identify a new intermediate stage in platelet production, the preplatelet, (c) delineate the cytoskeletal mechanics involved in preplatelet/proplatelet interconversion, and (d) model proplatelet fission and platelet release. Preplatelets are anucleate discoid particles 2-10 \(\mu\)m across that have the capacity to convert reversibly into elongated proplatelets by twisting microtubule-based forces that can be visualized in proplatelets expressing GFP-\(\beta\)1-tubulin. The ...
In patients with thrombocytopenia, it can be difficult to predict a patients bleeding risk based on platelet count alone. Platelet reactivity may provide additional information; however, current clinical assays cannot reliably assess platelet function in the setting of thrombocytopenia. New methods to study platelet reactivity in thrombocytopenic samples are needed. In this study, we sought to develop a laboratory model of thrombocytopenia using blood from healthy subjects that preserves the whole blood environment and reproducibly produces samples with a specific platelet count and hematocrit. We compared the activation state of unstimulated and agonist-stimulated platelets in thrombocytopenic samples derived from this method with normocytic controls. Whole blood was diluted with autologous red blood cell concentrate and platelet-poor plasma, which were obtained via centrifugation, in specific ratios to attain a final sample with a predetermined platelet count and hematocrit. P-selectin exposure and
TY - JOUR. T1 - Functional alterations of human platelets following 111In labeling with different ligands and incubation media. AU - Mieno, M.. AU - Isaka, Y.. AU - Kimura, K.. AU - Matsumoto, M.. AU - Etani, H.. AU - Uehara, A.. AU - Hashikawa, K.. AU - Hata, R.. AU - Moriwaki, H.. AU - Ashida, K.. AU - Imaizumi, M.. AU - Kamada, T.. AU - Kozuka, T.. PY - 1990/1/1. Y1 - 1990/1/1. N2 - We studied the effects of various 111In-water soluble chelates and incubation media on labeling efficiency of platelets and in vitro platelet aggregability. High labeling efficiency of platelets in ACD-saline was achieved with 111In-oxine sulfate, 111In-tropolone and 111In-MPO (2-mercaptopyridine-N-oxide). In the condition with 4.8 x 106/mm3 platelets in ACD-plasma, 111In-oxine-sulfate had low labeling efficiency and inconsistent labeling, while 111In-tropolone and 111In-MPO had high labeling efficiency. In vitro platelet aggregability (ADP 2 μM) was reduced when platelets were labeled in the absence of plasma. ...
With platelet activation, there is modulation of platelet surface molecule expression. In flow cytometric analyses of in vivo platelet activation, results are often confounded by activation induced in vitro by the preparative procedures. It is particularly important therefore to prevent or retard platelet activation as soon as possible after withdrawal of the blood sample. Taking blood into paraformaldehyde, or fixing the cells with paraformaldehyde as soon as possible after withdrawal, has been employed to prevent platelet activation in vitro, but paraformaldehyde-fixed platelets cannot be further used in functional studies. We investigated the efficacy of Diatube-H, a commercially available combination of platelet antagonists (theophylline, adenosine, and dipyridamole), in preventing or retarding platelet activation in vitro, along with its effects on modulation of platelet membrane glycoproteins (GP) and adhesion molecules. In contrast to blood taken into EDTA, blood taken into Diatube-H ...
The present study clarifies several aspects of the effects on platelet aggregation of arising and assuming the upright posture in the morning: 1) On arising, increased platelet aggregation can readily be observed in whole blood; 2) this increased aggregation is not accompanied by platelet activation, as evidenced by changes in activation-dependent markers on the platelet surface; 3) the observed increase in aggregation in whole blood may be partly explained by increases in platelet count and hematocrit that accompany arising. In addition, the study confirmed previous reports of increased fibrinolysis on standing and provided new evidence of an opposing increase in thrombin generation on standing.. Comparison with previous studies. Studies reporting the effects of arising in the morning on platelet aggregation (7-11,13) have exclusively studied aggregation in PRP, and to our knowledge, the present study is the first to report the effects of arising on platelet aggregation in whole blood. Of the ...
FIG. 2. Cleavage of α2-8-linked sialic acid from platelet surfaces results in a reduction of S. mitis binding. Binding of SF100 (□) to untreated human platelets (lane 1) or platelets pretreated with sialidase A (lane 2), sialidase V (lane 3), or sialidase C (lane 4). Binding of PS344 (▪) to platelets (lane 5) or platelets pretreated with sialidase A (lane 6), sialidase V (lane 7), or sialidase C (lane 8). Values presented are expressed as a percentage of wild-type binding to untreated platelets (mean ± SD). Data represent three experiments performed in triplicate on different occasions from a different donor each time. Note that these donors are different from those shown in Fig. 1. ...
Normal primary platelet aggregation requires agonist-mediated activation of membrane GPIIb-IIIa, binding of fibrinogen to GPIIb-IIIa, and cellular events after ligand binding. PAC1 monoclonal antibody distinguishes between resting and activated states of GPIIb-IIIa, and other antibodies preferentially recognize GPIIb (PMI-1) or IIIa (anti-LIBS1) after the binding of fibrinogen or fibrinogen-mimetic peptides, such as GRGDSP. Using these antibodies and platelet flow cytometry, we studied two distinct persistent platelet aggregation abnormalities. Platelets from a thrombasthenic variant, which contained near-normal amounts of GPIIb-IIIa, failed to aggregate or bind PAC1 in response to agonists. In addition, GRGDSP, which binds to normal GPIIb-IIIa without prior cell activation, failed to increase the binding of PMI-1 or anti-LIBS1 to the thrombasthenic platelets, suggesting a primary defect in ligand binding. Chromatography of detergent-solubilized platelets on a KYGRGDS affinity column confirmed ...
Platelets are cell fragments which are mainly an essential component when it comes to blood clotting and coagulation. The platelets along with the red blood cells and white blood cells, function together for the regulation and normal functioning of blood in our body. The blood cells have each of their own different life span as well as different functions. Among these three, the platelets are the smallest but one of the most important factors in blood clotting. If the platelets dont get regulated properly, such as when they dont get renewed at normal intervals, then the blood dysfunction or disorder might occur.. The general lifespan of a platelet is about 10 days. The normal platelet count in the human blood ranges from 150,000 to 450,000 per micro liter of blood. The platelet count varies in different locations and organs. But in cases of medical conditions, the platelets count may either increase or decrease in the blood. One of the most common condition of platelet count changes is the ...
SUMMARY Acquired qualitative platelet disorders are frequent causes of abnormal platelet function measured in vitro, although by themselves are usually associated with little or no clinical bleeding. However, there are important exceptions. Nevertheless, their major clinical impact becomes apparent in the additional presence of thrombocytopenia, or additional acquired or congenital disorders of hemostasis. Acquired disorders of platelet function can be conveniently classified into those that result from drugs, hematologic diseases, and systemic disorders. Drugs are the most frequent cause of acquired qualitative platelet dysfunction. Aspirin is the most notable drug in this regard because of its frequent use, its irreversible effect on platelet prostaglandin synthesis, and its documented effect on hemostatic competency, although this effect is minimal in normal individuals. Other nonsteroidal antiinflammatory drugs reversibly inhibit platelet prostaglandin synthesis and usually have little ...
Platelets are an important blood component that helps in controlling bleeding. The most abundant particle in our blood is the platelet that causes the blood to clot whenever theres a cut or injury, thereby stopping the bleeding in minutes. Now, there are tens and thousands of platelets in normal which help in this process. But in cases of certain diseases and illness like dengue etc the platelets gets destroyed by the infective germ, making the body prone to bleeding. Such a low count of platelet can cause serious brain damage and other organ damage. Apart from a blood transfusion, the most trusted way to increase your platelet count is by eating low platelets treatment food. Foods to increase platelet count are often used as basic preventive care and home remedy for diseases like dengue. But how do these home remedies to increase platelets work is a very basic understanding of…. ...
A low blood platelet count, known as thrombocytopenia, prevents blood from clotting normally. This can be an indication of serious medical conditions.SignificanceThe three main reasons for a low blood platelet count include reduced platelet production at bone marrow, blockage of platelets at enlarged spleen and an overuse or elimination of platelets by the body.Conditions Affecting Bone MarrowHIV and cancers such as leukemia and lymphoma reduce the capacity of bone marrow to produce platelets.Conditions Affecting the SpleenMyelofibrosis and certain forms of cirrhosis can enlarge the spleen. This hinders the passage of platelets into the bl...
While the heterozygous Q43P β1-tubulin carriers have a reduced function, the β1-tubulin-deficient mice present with only minor abnormalities in platelet hemostatic functions. Besides the fact that human platelets are more easy to handle and study in detail than mouse platelets, this can probably be explained by the fact that the loss of β1-tubulin expression in mouse platelets was overcome by overexpression of the other platelet β-tubulin variants,7 while the Q43P carrier platelets not only show reduced β1-tubulin but also total β-tubulin protein levels. In addition, incorporation of GFP-tagged Q43P β1-tubulin into wild-type tubulin structures seems to be inefficient and delocalized.. Due to the platelet dysfunction phenotype, the Q43P β1-tubulin variant could not only be conceived as a genetic risk factor for the development of thrombocytopenia but also as a protective genetic factor against cardiovascular disease. Indeed, a case-control study showed that the prevalence of Q43P ...
Activated blood platelets mediate the primary response to vascular injury. Although molecular abnormalities of platelet proteins occur infrequently, taken collectively, an inherited platelet defect accounts for a bleeding diathesis in ≈1:20,000 individuals. One rare example of a platelet disorder, Glanzmann thrombasthenia (GT), is characterized by life-long morbidity and mortality due to molecular abnormalities in a major platelet adhesion receptor, integrin αIIbβ3. Transfusion therapy is frequently inadequate because patients often generate antibodies to αIIbβ3, leading to immunemediated destruction of healthy platelets. In the most severe cases allogeneic bone marrow transplantation has been used, yet because of the risk of the procedure it has been limited to few patients. Thus, hematopoietic stem cell gene transfer was explored as a strategy to improve platelet function within a canine model for GT. Bleeding complications necessitated the use of a mild pretransplant conditioning ...
Significance: Levels of platelet noncoding RNAs (ncRNAs) are altered by disease, and ncRNAs may exert functions inside and outside of platelets. Their role in physiologic hemostasis and pathologic thrombosis remains to be explored. Recent Advances: The number of RNA classes identified in platelets has been growing since the past decade. Apart from coding messenger RNAs, the RNA landscape in platelets comprises ncRNAs such as microRNAs, circular RNAs, long ncRNAs, YRNAs, and potentially environmentally derived exogenous ncRNAs. Recent research has focused on the function of platelet RNAs beyond platelets, mediated through protective RNA shuttles or even cellular uptake of entire platelets. Multiple studies have also explored the potential of platelet RNAs as novel biomarkers. Critical Issues: Platelet preparations can contain contaminating leukocytes. Even few leukocytes may contribute a substantial amount of RNA. As biomarkers, platelet RNAs have shown associations with platelet activation, but ...
Activation of platelets with thrombin and other agonists causes a rapid increase in the phosphorylation of multiple proteins on tyrosine. To identify candidate protein-tyrosine kinases (PTKs; EC that may be responsible for these phosphorylation events, we analyzed the expression of seven Src-family PTKs and examined the association of these kinases with known platelet membrane glycoproteins. Five Src-related PTKs were detected in platelets: pp60SRC, pp60FYN, pp62YES, pp61HCK, and two LYN products of Mr 54,000 and 58,000. The Fgr and Lck PTKs were not detected. Although strict comparative quantification of protein levels was not possible, pp60SRC was detected at higher levels than any of the other kinases. In addition, glycoprotein IV (GPIV, CD36), one of the major platelet membrane glycoproteins, was associated in a complex with the Fyn, Yes, and Lyn proteins in platelet lysates. Similar complexes were also found in two GPIV-expressing cell lines, C32 melanoma cells and HEL cells. ...
Phosphatidylinositol 3-kinaseβ (PI3Kβ) plays a predominant role in integrin outside-in signaling and in platelet activation by GPVI engagement. We have shown that the tyrosine kinase Pyk2 mediates PI3Kβ activation downstream of integrin αIIbβ3, and promotes the phosphorylation of the PI3K-associated adaptor protein c-Cbl. In this study, we compared the functional correlation between Pyk2 and PI3Kβ upon recruitment of the two main platelet collagen receptors, integrin α2β1 and GPVI. PI3Kβ-mediated phosphorylation of Akt was inhibited in Pyk2-deficient platelets adherent to monomeric collagen through integrin α2β1, but occurred normally upon GPVI ligation. Integrin α2β1 engagement led to Pyk2-independent association of c-Cbl with PI3K. However, c-Cbl was not phosphorylated in adherent platelets, and phosphorylation of Akt occurred normally in c-Cbl-deficient platelets, indicating that the c-Cbl is dispensable for Pyk2-mediated PI3Kβ activation. Stimulation of platelets with CRP, a ...
TY - JOUR. T1 - Potentiation of TRAP-6-induced platelet dense granule release by blockade of P2Y12 signaling with MRS2395. AU - Mitrugno, Annachiara. AU - Rigg, Rachel A.. AU - Laschober, Nicole B.. AU - Ngo, Anh T.P.. AU - Pang, Jiaqing. AU - Williams, Craig D.. AU - Aslan, Joseph E.. AU - McCarty, Owen J.T.. N1 - Funding Information: This work was supported by grants from the National Institutes of Health (R01HL101972 and R01GM116184 to O.J.T.M.), the American Heart Association (13EIA12630000 to O.J.T.M. and 17SDG33350075 to J.E. A.) and the Altarum Institute (C.D.W. and O.J.T.M.). N.B.L. is a Johnson scholar. R.A.R is a Whitaker International Scholar. Funding Information: Super resolution microscopy studies were supported in part by the M.J. Murdock Charitable Trust. Publisher Copyright: © 2018 Taylor & Francis. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.. PY - 2018/5/19. Y1 - 2018/5/19. N2 - The release of ADP from platelet dense granules and its binding to platelet P2Y12 ...
Angiotensin II (Ang II) is a critical component of the reninangiotensin system that contributes to hypertension. Although platelets in blood from hypertensive subjects have an abnormal biological profile, it is unclear if circulating Ang II influences platelet aggregation or thrombus formation. One of the abnormalities presented to the platelets during hypertension is an elevated plasma concentration of serotonin (5-HT) caused by reduced 5-HT uptake secondary to loss of the 5-HT transporter (SERT) on the platelet plasma membrane. In the current study, we evaluated in vivo platelet function after 7 days of subcutaneous Ang II infusion to establish hypertension in mice and additionally assessed the biology of isolated platelets exposed to Ang II in vitro. The administration of Ang II elevated systolic blood pressure, but markers of platelet activation including P-selectin and PEJon/A staining were _disibledevent=font-size:10pt;line-height:1.5;font-family:Verdana;>5-HT in platelets, an event
Results: 22 hypoxemic patients were selected for the study based on their diagnosis of COPD (from history and spirometry) along with age and sex matched controls. Presence of comorbidities and other factors that cause platelet activation were excluded. Level of platelet aggregation was determined by several experiments. By an aggregometer using platelet agonists (thrombin and ADP) it was found that platelet aggregation was significantly higher in hypoxemic COPD patients than normal healthy controls. Fluorescence spectrophotometer was used to measure intracellular calcium as a marker of platelet activation and it was found that hypoxemic COPD patients had significantly higher platelet aggregation than normal healthy controls. However no significant difference was found in other markers of platelet activation studied namely P-selectin exposure and PAC-1 binding between the two groups ...
ABSTRACT. Angiotensin II (Ang II) is a critical component of the renin-angiotensin system that contributes to hypertension. Although platelets in blood from hypertensive subjects have an abnormal biological profile, it is unclear if circulating Ang II influences platelet aggregation or thrombus formation. One of the abnormalities presented to the platelets during hypertension is an elevated plasma concentration of serotonin (5-HT) caused by reduced 5-HT uptake secondary to loss of the 5-HT transporter (SERT) on the platelet plasma membrane. In the current study, we evaluated in vivo platelet function after 7 days of subcutaneous Ang II infusion to establish hypertension in mice and additionally assessed the biology of isolated platelets exposed to Ang II in vitro. The administration of Ang II elevated systolic blood pressure, but markers of platelet activation including P-selectin and PEJon/A staining were not changed. However, the aggregation response to collagen was reduced in isolated ...
Glycoprotein Ib-IX-V (GPIb-IX-V) is a platelet adhesion receptor complex that initiates platelet aggregation. Glycoprotein Iba (GPIba) is the central component of the GPIb-IX-V complex, anchoring the complex to the cytoskeleton and harboring the binding site for von Willebrand factor (vWF). Previous studies suggest that the coagulation function in pigs differs from that in humans, especially with respect to the interaction between vWF and platelets. However, we have little knowledge about the function of porcine platelets, which is important with regard to studies of cardiovascular disease, clotting, and surgery that use pigs as animal models. To extend this information, we cloned and analyzed the porcine GPIba sequence. Porcine GPIba contains 1891 nucleotides and includes an open reading frame that encodes 627 amino acids. The nucleotide sequence showed 67% identity with human GPIba, whereas the deduced amino acid sequences were 59% identical. The vWF binding domain shares the highest identity ...
BioAssay record AID 336312 submitted by ChEMBL: Antiplatelet activity against bovine citreated platelet assessed as inhibition of ADP-induced platelet aggregation up to 278 ug/ml after 6 mins.
TY - JOUR. T1 - Extensive characterization of the composition and functional activities of five preparations of human platelet lysates for dedicated clinical uses. AU - Delila, Liling. AU - Wu, Yu Wen. AU - Nebie, Ouada. AU - Widyaningrum, Rifa. AU - Chou, Ming Li. AU - Devos, David. AU - Burnouf, Thierry. N1 - Funding Information: LD and ON were supported by MS and PhD fellowships from Taipei Medical University, and RW by PhD fellowship from Ministry of Education of Taiwan. Publisher Copyright: © 2020 Taylor & Francis Group, LLC. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.. PY - 2021. Y1 - 2021. N2 - Human platelet lysates (HPLs), rich in various growth factors and cell growth-promoting molecules, encompass a new range of blood products that are being used for regenerative medicine, cell therapies, and tissue engineering. Well-characterized dedicated preparations, tailor-made to best fit specific therapeutic applications, are needed for optimal clinical efficacy and safety. ...
To delineate the critical top features of platelets necessary for balance and formation of thrombi, thromboelastography and platelet aggregation measurements were employed in whole blood of normal individuals and of those with Bernard-Soulier Syndrome (BSS) and Glanzmanns Thrombasthenia (GT). generation of stable thrombi, a potentially significant feature in individual medical results. Introduction An initial step in thrombus formation in the hurt vascular endothelium is the adhesion of platelets to shown subendothelial elements, e.g., von Willebrand Aspect (vWF), under high prices of shear, via the connections from the platelet glycoprotein (GP) 1b/V/IX receptor complicated with subendothelial vWF [1]. This tethering of platelets after that promotes their firmer binding to subendothelial collagen (COL) fibres via platelet receptors, e.g., GPVI [2], [3] and integrin II1 [4]. In this procedure, platelets are turned on, resulting in platelet shape adjustments, aggregation, discharge of aggregation ...
Octadecadienoic acids (linoleic acid and linolelaidic acid) and the diacylglycerol, 1-oleoyl-2-acetyl-rac-glycerol (OAG) concentration-dependently induced activation of gel-filtered human platelets, i.e. aggregation and phosphorylation of 20 kDa and 47 kDa peptides. In contrast, octadecenoic acids (oleic and elaidic acid) and octadecanoic (stearic) acid were inactive. Octadecadienoic acid-induced platelet activation was suppressed by the protein kinase C inhibitor, polymyxin B, but not by the cyclooxygenase inhibitor, indomethacin. OAG-induced activation was potentiated by octadecadienoic acids present at non-stimulatory concentrations. Our data suggest that octadecadienoic acids and diacylglycerol synergistically induce platelet activation via protein kinase C. Furthermore, linolelaidic acid may provide a useful experimental tool to study fatty acid regulation of protein kinase C in intact cells. ...
Staphylococcus aureus is an opportunistic pathogenic bacterium known for its ability to interact with platelets and modulate their function. S. aureus lipoproteins are one of the major groups of bacterial surface molecules and are released into the extracellular milieu where they are recognized by host immune cells. The aim of this study was to determine the role of S. aureus lipoproteins in S. aureus-platelet interactions. Platelet aggregation and binding assays using S. aureus wild type and lgt strains showed that, S. aureus lipoproteins contribute towards binding of the pathogen to platelets. Lipoproteins present in extracellular milieu also bind platelets. Platelet spreading, thrombus formation, agonist induced platelet aggregation and αIIbβ3 activation were inhibited by cell-free lipoproteins. CD36 was identified as the major platelet surface molecule interacting with S. aureus lipoproteins. Antibody neutralization demonstrated that functional inhibition of platelet activation caused by ...
Seventy-five consecutive patients with normal platelet counts were investigated for easy bruising. All had a normal coagulation profile, and all except four were women. None were on aspirin or other antiplatelet agents. Two specific groups could be delineated. In type I (44 patients, mean age, 35), platelet function was normal to supranormal. Megathrombocyte number was elevated in 60% of patients and correlated with the presence of antiplatelet antibody in 30% of patients. In type II (31 patients, mean age, 34), platelet function was abnormal: impaired epinephrine aggregation (primary and secondary wave) in 97%, impaired connective tissue aggregation in 77%, and impaired ADP aggregation in 42%. Megathrombocyte number was elevated in 71%, and antiplatelet antibody was present in 38% of patients. The easy bruising syndrome can be differentiated into two categories: type I, in which a platelet abnormality is unlikely, and type II, in which a platelet abnormality exists. Elevated incidence of ...
The work herein examines in vitro platelet aggregation in response to fluid shearing motion. Our specific aim is to characterize shear-induced aggregation by means of kinetic measurements. In doing so we consider plausible physicochemical mechanisms for platelet activation in the shear field. Besides resolving some questions concerning the activation of platelets by shear forces, this study further implicates fluid mechanical factors in thrombosis and arterial disease. Specific results may also apply to the design and evaluation of blood-contacting artificial devices. The experimental procedure centers on the use of a rotational viscometer to apply a controlled shearing motion to platelet suspensions for prescribed times. We quantify aggregation through changes in particle size histograms and associated measures (e.g. total number of particles). Additional insight into the aggregation response comes from interpreting kinetic data using the coalescence equation, a population balance specific for ...
It has been proposed that adsorbed glycoproteins such as fibrinogen and gamma-globulin induce platelet adhesion at blood-polymer interfaces. The importance of oligosaccharide groups in the glycoproteins proved to be responsible for platelet adhesion and aggregation via possible complex formation. Several studies have provided evidence that the proposed mechanism was involved in platelet adhesion on polymer surfaces. To minimize or prevent platelet adhesion on polymers, prostaglandins (PGs), potent inhibitors of platelet aggregation and PG-heparin (HEP) conjugate, were combined with polymers via physical dispersion or chemical immobilization on the surfaces. Albumin-HEP conjugate-adsorbed surfaces also showed significant reduction of platelet adhesion.
Human platelet-rich plasma (PRP) was irradiated in vitro with a fiberoptic Nd:YAG laser-heated metal cap to study its effects on platelets. The energy of the laser was 5 and 10 watts with an irradiation time of 0, 3, 6, and 9 seconds and 14 watts with an irradiation time of 0, 3, 4, and 5 seconds, respectively. The irradiated PRPs were analyzed for platelet count, aggregation reaction, thromboxane (TX)B2 measurement and electron microscopy. Various degrees of decrease in platelet count were observed in all groups. Except the 5Wx3S group, the other groups showed an increase in the maximum aggregation rate of platelets, which corresponded to the enhancement of TXB2 formation. It was also demonstrated by a transmission electron microscopy in 10Wx3S, 10Wx6S, 10Wx9S, 14Wx3S, 14Wx4S, and 14Wx5S energy groups that alpha- and dense-particles in irradiated platelets became sparse in number or even disappeared, less electron density, irregularity in size and shape, and a tendency for these particles to ...
This study was conducted to examine the mechanism(s) of synergistic interaction of adrenaline and platelet-activating factor (PAF) mediated human platelet aggregation. We found that platelet aggregation mediated by subthreshold concentrations of PAF (5-8 nM) plus adrenaline (0.5-2 μM) was inhibited by both α2-adrenoceptor blocker (yohimbine) and PAF receptor antagonist (WEB2086). While examining the role of the downstream signalling pathways, we found that this synergism was inhibited by calcium channel blockers, verapamil, and diltiazem. In addition, platelet aggregation by co-addition of adrenaline and PAF was also inhibited by very low concentrations of phospholipase C (PLC) inhibitor (U73122; IC50 = 0.2 μM), the MAP kinase inhibitor, PD98059 (IC50 = 3 μM) and cyclooxygenase (COX-1) inhibitors including indomethacin (IC50 = 0.25 μM), flurbiprofen (IC50 = 0.7 μM) and piroxicam (IC50 = 7 μM). However, the COX-2 inhibitor, nimesulide, was also effective in inhibiting the aggregation. The
The latter work was performed with murine species and, of note, many differences have been emphasized in megakaryocyte localization and ultrastructure of proplatelet formation, between mice and humans.32 The present study performed on human MKs sheds additional light on how these large protrusions can be induced to fragment into small platelets by shear forces in flowing blood. This is also in keeping with evidence that the pulmonary circulation could be an important site of platelet production, as the lung capillaries would be the first to be encountered by cells leaving the bone marrow.28 Indeed, the large cytoplasmic fragments and the isolated platelet-sized fragments that we observed in real time to form on the coverslip during the flow assay resembled those seen downstream of the pulmonary circulation in vivo.33 In our conditions, high shear rates were essential to proplatelet and platelet formation during an exposure time of 20 minutes. In contrast, no proplatelet or platelet was generated ...
REPORT DESCRIPTIONPlatelet rich plasma (PRP) is a blood plasma product in which concentration of platelets is elevated four to eight times higher than the normal blood platelet concentration. PRP is also called platelet rich gel, platelet enriched plasma and platelet enriched gels; and is used in effective treatment...
What Discussion about plateletsThrombocytopenia, platelet count, normal platelet count, high platelet count, low platelets, and other aspect
T cell depletion with antithymocyte globulins (ATG) can be complicated by thrombopenia and. hypercoagulability. The underlying mechanism is still unclear. We found that binding of ATG to. platelets caused platelet aggregation, alpha-granule release, membrane phosphatidylserine exposure. and the rapid release of platelet microvesicles (MV). Platelet activation and MV release. were complement-dependent and required membrane insertion of C5b-8 but not stable lytic pore. formation by C5b-9. Full platelet aggregation and activation by ATG also required the low affinity. Fc gamma receptor FcγRII. MV release, however, was FcγRII-independent. Platelet MV. expressed high prothrombinase activity. Moreover, blocking C5 inhibited ATG-induced thrombin. generation in platelet rich plasma. In 19 hematopoietic stem cell and kidney transplant patients,. ATG treatment resulted in thrombopenia and increased plasma levels of d-dimer and thrombin-anti-. thrombin-complexes. Flow cytometric analysis of complement ...
Blood platelet activation[edit]. Under normal conditions, small disk-shape platelets circulate in the blood freely and without ... ADP is stored in dense bodies inside blood platelets and is released upon platelet activation. ADP interacts with a family of ... ADP in the blood is converted to adenosine by the action of ecto-ADPases, inhibiting further platelet activation via adenosine ... P2Y1 receptors initiate platelet aggregation and shape change as a result of interactions with ADP. ...
2005). "WAVE/Scars in platelets". Blood. 105 (8): 3141-8. doi:10.1182/blood-2003-04-1319. PMID 15280206. Kashiwagi H, Shiraga M ... 2005). "Expression and subcellular localization of WAVE isoforms in the megakaryocyte/platelet lineage". J. Thromb. Haemost. 3 ...
Stoffel W, Heimann G, Hellenbroich B (1973). "Sphingosine kinase in blood platelets". Hoppe-Seyler's Z. Physiol. Chem. 354 (5 ...
Nugteren, D. H. (1975). "Arachidonate lipoxygenase in blood platelets". Biochimica et Biophysica Acta (BBA) - Lipids and Lipid ... Sub-primate mammals, such as the mouse, rat, rabbit, cow, and pig, express platelet type 12-lipoxygenase but also a leukocyte ... It was first found as a product of arachidonic acid metabolism made by human and bovine platelets through their 12S- ... Thus, the production of hepoxilins from 12(S)-HpETE may result from the intrinsic activity of platelet or leukocyte type 12- ...
Based predominantly on the presence of its mRNA, human ALOX12 is distributed predominantly in blood platelets and leukocytes ... Nugteren DH (February 1975). "Arachidonate lipoxygenase in blood platelets". Biochimica et Biophysica Acta (BBA) - Lipids and ... "Immunocytochemical localization of platelet-type arachidonate 12-lipoxygenase in mouse blood cells". The Journal of ... regulate regional blood flow, and contribute to the regulation of blood pressure in animal models (see Hepoxilins). Far more ...
When the lining of a blood vessel is broken, platelets are attracted, forming a platelet plug. These platelets have thrombin ... Blood platelets: biochemistry and physiology]". Hamostaseologie (in German). 23 (4): 149-58. doi:10.1055/s-0037-1619592. PMID ... the blockage of a vessel by an agglutination of red blood cells, platelets, polymerized fibrin and other components. ..., Defibrinated blood harvested from sheep (video) Fibrin: Molecule of the Month, by David Goodsell, RCSB Protein ...
Wright, J. H. (1910). "The histogenesis of blood platelets". Journal of Morphology. 21 (2): 263-78. doi:10.1002/jmor.1050210204 ... Platelets are first named by James Homer Wright. Peyton Rous demonstrates that a malignant tumor can be transmitted by a virus ... Chicago cardiologist James B. Herrick makes the first published identification of sickle cells in the blood of a patient ... Herrick, James B. (November 1910). "Peculiar elongated and sickle-shaped red blood corpuscles in a case of severe anemia". ...
"Tachykinins regulate the function of platelets". Blood. 104 (4): 1058-65. doi:10.1182/blood-2003-11-3979. PMID 15130944. Page ...
It is also expressed blood platelets. VMAT2 is also co-expressed in chromaffin cells. Expression of the two transporters in ... Activated heterotrimeric G-protein Gαq downregulates VMAT2 mediated serotonin transport in blood platelets, but this is not the ... May 2003). "The vesicular monoamine content regulates VMAT2 activity through Galphaq in mouse platelets. Evidence for ...
First accurate counts of blood platelets. Traitement du choléra, G. Masson, Paris, 1885 - Treatment of cholera. Du sang et ses ... He performed the first accurate count of blood platelets, and is credited with developing a solution of mercury bichloride, ... Lecons cliniques sur les maladies du sang, G. Masson, Paris, 1900 - Clinical lessons on blood disorders. L'hématoblaste, ... sodium chloride and sodium sulfate for dilution of blood prior to counting erythrocytes with a hemocytometer. In 1874 he ...
Thrombocytopenia (low blood platelets) is unusual. The peripheral blood and bone marrow aspirate findings in copper deficiency ... Bone marrow aspirate in both conditions may show dysplasia of blood cell precursors and the presence of ring sideroblasts ( ... Copper deficiency can have many hematological consequences, such as myelodysplasia, anemia, low white blood cell count, and low ... when a blood profile has indicators of possible future leukemia development), but it was not until 2001 that copper deficiency ...
blood platelet aggregation; blood clotting; allergic reactions. NSAIDs inhibit its production to reduce incidence of strokes ... The blood vessels engorge and the injury reddens.. Swelling-LTB4 makes the blood vessels more permeable. Plasma leaks out into ... and other blood vessels as well as on the kidney's reabsorption of sodium and water, and act to reduce blood pressure and ... blood flow to tissues, and/or blood pressure. However, their function and relevancy to human physiology and pathology have not ...
Megakaryocytes release platelets into the bloodstream. Platelets are critical for normal blood clotting. In consequence of this ... Blood samples are obtained from the fetal umbilical cord to determine blood cell counts, measure blood enzymes to evaluate ... decreased numbers of circulating platelets and red blood cells, and increased numbers of circulating white blood cells. Also ... reduced platelet production often accompanied by significantly reduced levels of circulating platelets; reduced red blood cell ...
Preservation of Blood Platelets. US Patent Number 5,876,676, issued March 2, 1999. 7. Stossel, TP, Hartwig, JH, Hoffmeister, KM ... Effect of corticosteriod therapy on the phagocytosis of antibody-coated platelets by human leukocytes. Blood. 1978; 51:771-779 ... The clearance mechanism of chilled blood platelets. 2003. Cell, 112: 87-97. 126. Hoffmeister, KM, Josefsson, EC, Isaac, NA, ... Glycosylation restores survival of chilled blood platelets. 2003. Science 301: 1532-1534. 127. Woo, MS, Ohta, Y, Rabinovitz, I ...
The blood components are from a family donor. HLA-matched platelets are transferred. Another means of prevention is the use of ... Transfusion-associated graft-versus-host disease (TA-GvHD) is a rare complication of blood transfusion, in which the donor T ... The incidence of TA-GvHD in immunocompromised patients receiving blood transfusions is estimated to be 0.1 - 1.0%, and ... Prevention includes gamma irradiation of the lymphocyte-containing blood products. This procedure should be performed in ...
... specifically the platelets in the blood. Platelets are cell fragments in the blood that aid in clotting. Platelets are produced ... Normal platelet counts range from 150,000 to 450,000 platelets per µL of blood. Individuals with XLT usually have drastically ... reduced platelet counts, typically less than 70,000 platelets per µL of blood. Not only are there fewer platelets circulating, ... but individuals with XLT also have smaller platelets. Fewer and smaller platelets causes the efficacy of the clotting mechanism ...
... or overproduction of blood platelets. It also has been used in the treatment of chronic myeloid leukemia. Anagrelide controlled ... Anagrelide works by inhibiting the maturation of platelets from megakaryocytes. The exact mechanism of action is unclear, ... age over 60 years platelet count over 1000×109/L a history of thrombosis According to a 2005 Medical Research Council ...
Fresh venous clots are red blood cell and fibrin rich. Platelets and white blood cells are also components. Platelets are not ... Individuals without O blood type have higher blood levels of von Willebrand factor and factor VIII than those with O blood type ... NETs provide "a scaffold for adhesion" of platelets, red blood cells, and multiple factors that potentiate platelet activation ... Blood has a natural tendency to clot when blood vessels are damaged (hemostasis) to minimize blood loss. Clotting is activated ...
1990). "Gova/b alloantigen system on human platelets". Blood. 75 (11): 2172-6. doi:10.1182/blood.V75.11.2172.2172. PMID 2346781 ... 1991). "Identification of a cell-surface antigen associated with activated T lymphoblasts and activated platelets". Blood. 77 ( ... 2002). "A tyrosine703serine polymorphism of CD109 defines the Gov platelet alloantigens". Blood. 99 (5): 1692-8. doi:10.1182/ ... and activated platelets (Lin et al., 2002). In addition, the platelet-specific Gov antigen system (HPA15), implicated in ...
Her blood vessels were largely filled with platelets. Modern reports still occasionally refer to TTP as "Moschcowitz disease" ...
MAO-B is mostly found in blood platelets. MAO-A appears at roughly 80% of adulthood levels at birth, increasing very slightly ... Domino EF, Khanna SS (March 1976). "Decreased blood platelet MAO activity in unmedicated chronic schizophrenic patients". The ... Schildkraut JJ, Herzog JM, Orsulak PJ, Edelman SE, Shein HM, Frazier SH (April 1976). "Reduced platelet monoamine oxidase ... Oreland L (January 2004). "Platelet monoamine oxidase, personality and alcoholism: the rise, fall and resurrection". ...
Thaulow, E; Erikssen, J; Sandvik, L; Stormorken, H; Cohn, PF (August 1991). "Blood platelet count and function are related to ... Holmsen, H; Day, HJ; Stormorken, H (1969). "The blood platelet release reaction". Scandinavian Journal of Haematology. ... Gogstad, GO; Stormorken, H; Solum, NO (15 July 1983). "Platelet alpha 2-antiplasmin is located in the platelet alpha-granules ... Day, HJ; Stormorken, H; Holmsen, H (1973). "Subcellular localization of platelet factor 3 and platelet factor 4". Scandinavian ...
Duttaroy, Asim K. (June 2018). Human Blood Platelet Function: Applications in Cardiovascular Health. Wiley. pp. 1-300. ISBN 978 ... discovered that an extract from tomato had a positive effect in the prevention of blood platelet aggregation.[4][5][6] ... "Effects of antiplatelet components of tomato extract on platelet function in vitro and ex vivo: A time-course cannulation ...
Thrombocytopenia is a deficiency of platelets in the blood. It can present as red blood blisters in the mouth. Patients ... A heart attack is a blood vessel in the heart being constricted either by a blood clot or atherosclerosis formation. A heart ... High blood sugar can be detected by sampling saliva. Saliva sampling may be a non-invasive way to detect changes in the gut ... Sickle cell disease is a hereditary genetic condition that results in deformed red blood cells to be formed. Sickle patients ...
A decrease in blood platelets (thrombocytopenia) also may occur. Since platelets are important for the clotting of blood, ... One possible side effect is agranulocytosis, a decrease of white blood cells in the blood. Symptoms and signs of ... Other severe side effects include liver problems and low blood cell counts. Use during pregnancy may harm the baby. ...
"Dey's personal website". Barthel, W.; Markwardt, F. (1975). "Aggregation of blood platelets by adrenaline and its uptake". ...
Shattil SJ, Newman PJ (September 2004). "Integrins: dynamic scaffolds for adhesion and signaling in platelets". Blood. 104 (6 ... Blood. 105 (12): 4604-12. doi:10.1182/blood-2004-10-4093. PMC 1894992. PMID 15705783. Gueller S, Hehn S, Nowak V, Gery S, Serve ... doi:10.1182/blood-2004-04-1257. PMID 15205259. He X, Li Y, Schembri-King J, Jakes S, Hayashi J (August 2000). "Identification ... As a result, it is involved in blood diseases, autoimmune disorders, and vascular disease. The SH2B3 gene also contains one of ...
Barthel, W.; Markwardt, F. (1975-10-15). "Aggregation of blood platelets by adrenaline and its uptake". Biochemical ...
... platelets, into the circulation. Platelets are critical for the normal clotting of blood. While malignant megakaryoblasts ... increased blast cells in blood and/or bone marrow, immunochemical evidence that these blast cells bear platelet line-specific ... Blood. 100 (7): 2292-302. doi:10.1182/blood-2002-04-1199. PMID 12239137. de Rooij JD, Masetti R, van den Heuvel-Eibrink MM, ... Blood. 127 (20): 2391-405. doi:10.1182/blood-2016-03-643544. PMID 27069254. Seewald L, Taub JW, Maloney KW, McCabe ER ( ...
Blood products such as packed red blood cells, platelets, or fresh frozen plasma may also be used.[135] Other regulators of ... This may cause vomiting blood, coughing up of blood, or blood in stool.[32] Bleeding into the skin may create petechiae, ... Possible non-specific laboratory indicators of EVD include a low platelet count; an initially decreased white blood cell count ... Finding the virus, viral RNA, or antibodies in blood[1]. Differential diagnosis. Malaria, cholera, typhoid fever, meningitis, ...
Cord blood has a higher concentration of HSC than is normally found in adult blood. However, the small quantity of blood ... platelet and hemoglobin levels dip post-procedure, not returning to normal until after one month.[45] ... Umbilical cord blood[edit]. Umbilical cord blood is obtained when a mother donates her infant's umbilical cord and placenta ... Allogeneic cord blood is stored frozen at a cord blood bank because it is only obtainable at the time of childbirth. To ...
Platelet-derived growth factor (A, B, C, D). *Kinase inhibitors: Agerafenib. *Axitinib ... Low amount of potassium in the blood. *Conjunctivitis. *Increased ALT. *Increased AST ...
... or who have abnormally low levels of white or red cells or platelets in the blood, should be investigated for possible ... and possibly testing for specific antibodies in the blood.[3][11] Blood tests are often negative in the early stages of the ... Based on symptoms, tick exposure, blood tests[3]. Prevention. Prevention of tick bites (clothing the limbs, DEET), doxycycline[ ... Tests for antibodies in the blood by ELISA and Western blot is the most widely used method for Lyme diagnosis. A two-tiered ...
... binds to alpha-actinin-1 and associates with actin filaments and stress fibers in activated platelets and endothelial cells.". ... Blood. 96 (13): 4236-45. PMID 11110697. *Ostendorff HP، Peirano RI، Peters MA، Schlüter A، Bossenz M، Scheffner M، Bach I (2002 ...
Other laboratory findings in Lassa fever include lymphocytopenia (low white blood cell count), thrombocytopenia (low platelets ... Fluid replacement, blood transfusion, and fighting hypotension are usually required. Intravenous interferon therapy has also ... and elevated aspartate transaminase levels in the blood. Lassa fever virus can also be found in cerebrospinal fluid.[16] ... to avoid contact with blood and body fluids. These issues in many countries are monitored by a department of public health. In ...
Basic blood tests can be used to check the concentration of hemoglobin, platelets, sodium, potassium, chloride, bicarbonate, ... Blood products including intravenous immunoglobulin and a process known as plasma exchange can also be employed. ... An erythropoetin stimulating agent may be required to ensure adequate production of red blood cells, activated vitamin D ... Treatments in nephrology can include medications, blood products, surgical interventions (urology, vascular or surgical ...
... causes a doubling of the blood plasma levels of moclobemide.[8] Blood plasma levels of trimipramine and maprotiline and ... Platelet MAO is of the MAO-B and this is inhibited only to a small degree in humans; the inhibition is due to low levels of ... No significant rise in blood pressure occurs when moclobemide is combined with amines such as tyramine-containing foods or ... which cause a severe rise in blood pressure with such combination.[9] Due to the lack of anticholinergic, cardiovascular, ...
The procedure creates a blood clot scaffold on which injected PBPCs can be recruited and enhance chondrogenesis at the site of ... "Safety and complications reporting on the re-implantation of culture-expanded mesenchymal stem cells using autologous platelet ... microfracture surgery followed by postoperative injections of autologous peripheral blood progenitor cells (PBPCs) and ... "Articular cartilage regeneration with autologous peripheral blood progenitor cells and hyaluronic Acid after arthroscopic ...
Enumeration of CD4+ T-cells in the peripheral blood of HIV-infected patients: interlaboratory study of the FACSCount system. ... platelet activation in vascular disease and stem cell transplantation in cancer patients. As an endocrinologist he has an ... 1999 October 15;38(5):231-7. V. Chernyshov, E. Vykhovanets, I. Slukvin, Y. Antipkin, A. Vasyuk, K. Strauss, Analysis of blood ... Age-related changes in Human blood lymphocyte subpopulations. Clinical Immunology and Immunopathology, 1994; 70:152-158. K. ...
t-PA is released into the blood very slowly by the damaged endothelium of the blood vessels, such that, after several days ( ... Fibrinolysis is a process that prevents blood clots from growing and becoming problematic.[1] This process has two types: ... This may help to avoid the use of blood products such as fresh frozen plasma with its associated risks of infections or ... in whole blood, even in patients on heparin. In this assay, increased fibrinolysis is assessed by comparing the TEM profile in ...
wasting syndrome - Western blot - white blood cells - wild-type virus - window period - Women's Interagency HIV Study (WIHS) - ... platelets - PML - Pneumocystis jiroveci pneumonia (formerly Pneumocystis carinii or PCP) - POL - polymerase - Polymerase chain ... blood-brain barrier - body fat redistribution (BFR) syndrome - body fluids - bone marrow - bone marrow suppression - booster - ... complete blood count (CBC) - computed tomography scan (C-T scan) - concomitant drugs - condyloma - condyloma acuminatum - ...
... which distinguishes them from the other blood cells, the anucleated red blood cells (RBCs) and platelets. Types of white blood ... All white blood cells are nucleated, which distinguishes them from the anucleated red blood cells and platelets. Types of ... Histamine is responsible for widening blood vessels and increasing the flow of blood to injured tissue. It also makes blood ... "Blood. 96 (13): 4028-38. PMID 11110670.. *^ a b c d e f Kumar V, et al. (2010). Robbins and Cotran pathologic basis of disease ...
2009). "Universal adoption of pathogen inactivation of platelet components: impact on platelet and red blood cell component use ... in platelet and plasma blood components prepared for transfusion support of patients. Prior to clinical use, amotosalen-treated ... Water solubility is important for two reasons: pharmacokinetics relating to drug solubility in blood and necessitating the use ... An additional use for optimized psoralens is for the inactivation of pathogens in blood products. The synthetic amino-psoralen ...
APS provokes blood clots (thrombosis) in both arteries and veins as well as pregnancy-related complications such as miscarriage ... A low platelet count and positivity for antibodies against β2-glycoprotein 1 or phosphatidylserine may also be observed in a ... Kay Thackray (2003). Sticky Blood Explained. Braiswick. ISBN 978-1-898030-77-5.. A personal account of dealing with the ... Often, this disease is treated by giving aspirin to inhibit platelet activation, and/or warfarin as an anticoagulant. The goal ...
"Blood chemicals link' to eczema". BBC News. 26 August 2007.. *^ Shu XQ, Mendell LM (July 1999). "Neurotrophins and hyperalgesia ... Platelet-derived growth factor (A, B, C, D). *Kinase inhibitors: Agerafenib. *Axitinib ... "Blood levels of brain-derived neurotrophic factor correlate with several psychopathological symptoms in anorexia nervosa ...
... several knobby white blood cells including lymphocytes, a monocyte, a neutrophil, and many small disc-shaped platelets. ... White blood cells identify and remove foreign substances present in organs, tissues, blood and lymph. ... A scanning electron microscope image of normal circulating human blood. One can see red blood cells, ... All white blood cells (WBC) are known as leukocytes. Leukocytes are different from other cells of the body: they work like ...
Blood plasma without the plasma proteins, red blood cells, and platelets pass through the intercellular cleft and into the ... Continuous blood capillaries have the smallest intercellular clefts, with discontinuous blood capillaries having the largest ... Most notably, intercellular clefts are described in capillary blood vessels. The three types of capillary blood vessels are ... Intercellular clefts also play a role in the formation of the blood-heart barrier (BHB). The intercellular cleft between ...
লোহিত রক্তকণিকা (Red blood cell). *অণুচক্রিকা (Platelet). *রক্তরস (Plasma). লসিকা তন্ত্র ও. অনাক্রম্যতন্ত্র. *লসিকা (Lymph) ...
Blood 94 (6): 1878-89. PMID 10477716. Cite uses deprecated parameter ,month=. (help) ... resides in a gene cluster along with several other members of the platelet factor 4 gene superfamily". Hum. Genet. 84 (2): 185- ...
Xu W, Xie Z, Chung DW, Davie EW (1998). "A novel human actin-binding protein homologue that binds to platelet glycoprotein ... Ibalpha". Blood. 92 (4): 1268-76. PMID 9694715.. *. Bröcker F, Bardenheuer W, Vieten L, et al. (1999). "Assignment of human ...
The gut mucosal cells do not get enough nourishment from arterial blood supply to stay healthy, especially in very premature ... Laboratory changes (metabolic acidosis, too few platelets in the bloodstream). *Bell's stage 3 (advanced disease): *Severe ... The underlying mechanism is believed to involve a combination of poor blood flow and infection of the intestines.[2] Diagnosis ... blood in the stool, or vomiting of bile.[1][2] ... infants, where the blood supply is limited due to immature ...
... several knobby white blood cells including lymphocytes, a monocyte, a neutrophil, and many small disc-shape platelets. ... Identification and removal of foreign substances present in organs, tissues, blood and lymph, by specialized white blood cells ... A scanning electron microscope image of normal circulating human blood. One can see red blood cells, ... All white blood cells (WBCs) are known as leukocytes. Most leukocytes differ from other cells of the body in that they are not ...
... the formation of new blood vessels) and increased permeability (leakage from blood vessels), two of the primary pathological ... Platelet-derived growth factor (A, B, C, D). *Kinase inhibitors: Agerafenib. *Axitinib ... This then reduces the growth of the blood vessels located within the eye and works to control the leakage and swelling.[2] ... was also found that subcutaneous and intravenous routes of administration were also effective at maintaining the desired blood ...
... epidural blood patch, plasmapheresis, blood labeling or tagging and platelet gel (autologous) ... Rejection of blood transfusions. Main article: Jehovah's Witnesses and blood transfusions. Jehovah's Witnesses refuse blood ... Though Jehovah's Witnesses do not accept blood transfusions of whole blood, they may accept some blood plasma fractions at ... "The Real Value of Blood". Awake!. August 2006. p. 11.. *^ Durable Power of Attorney form. Watch Tower Society. January 2001. p ...
Roles of platelets and factor XI in the initiation of blood coagulation by thrombin. Thromb. Haemost.. July 2001, roč. 86, čís ... Amino acid sequence of human factor XI, a blood coagulation factor with four tandem repeats that are highly homologous with ...
In particular, PDGF plays a significant role in blood vessel formation, the growth of blood vessels from already-existing blood ... Though PDGF is synthesized,[3] stored (in the alpha granules of platelets),[4] and released by platelets upon activation, it is ... "The Basic Biology of Platelet Growth Factors". Retrieved 2014-05-08.. *^ Kumar, Vinay (2010). Robbins and Coltran Pathologic ... Platelet-derived growth factor (PDGF) is one among numerous growth factors that regulate cell growth and division. ...
It is a peptide that causes blood vessels to dilate (enlarge), and therefore causes blood pressure to fall. A class of drugs ... further lowering blood pressure. Bradykinin dilates blood vessels via the release of prostacyclin, nitric oxide, and ... "Hyperfibrinolysis increases blood-brain barrier permeability by a plasmin- and bradykinin-dependent mechanism". Blood. 128 (20 ... Bradykinin was detected in the blood plasma of animals after the addition of venom extracted from the Bothrops jararaca ( ...
... blood. 105 (8): 3178-84. doi:10.1182/blood-2004-10-3985. PMID 15626732. José RJ, Williams AE, Mercer PF, Sulikowski MG, Brown ... Molino M, Bainton DF, Hoxie JA, Coughlin SR, Brass LF (Feb 1997). "Thrombin receptors on human platelets. Initial localization ... Hoffman M, Church FC (Aug 1993). "Response of blood leukocytes to thrombin receptor peptides". Journal of Leukocyte Biology. 54 ... Blood. 89 (6): 1944-53. PMID 9058715. This article incorporates text from the United States National Library of Medicine, which ...
97 (9): 2633-9. doi:10.1182/blood.v97.9.2633. PMID 11313252.. *^ a b She HY, Rockow S, Tang J, Nishimura R, Skolnik EY, Chen M ... and small-sized platelets) in these patients the protein is usually significantly reduced or absent. Other, less inactivating ... blood coagulation. • positive regulation of Arp2/3 complex-mediated actin nucleation. • actin filament-based movement. • ...
Clinicians need to be aware of bacterial contamination of blood products, especially platelets. ... In practice, the type of platelet donation (apheresis or whole blood-derived platelets) has dictated the bacterial detection ... Clinician is contacted by the blood collection or transfusion service with information involving a blood or blood component ... Most blood collection centers culture apheresis platelets (derived from single donors), and release the unit after the culture ...
Hospitals and blood banks are adopting new measures to improve the safety of donated platelets-the tiny cells that make blood ... Hospitals and blood banks are adopting new measures to improve the safety of donated platelets-the tiny cells that make blood ... Unlike other blood components such as red cells, which are refrigerated, platelets must be stored at room temperature to remain ... A growing number of studies show that standard tests performed by blood banks before they ship platelets to hospitals miss the ...
Ive had low blood platelets for as long as I can remember.Even before I discovered I was Hep C+. I went thru tx( peg-intron- ... The hepatitis C virus (HCV) is spread by blood-to-blood contact with an infected persons blood. Many people with HCV infection ... or back up of blood flow. THE LARGE SPLEEN sequesters or HIDES platelets....hence the low platelets (thrombocytopenia) 3) The ... Ive had low blood platelets for as long as I can remember.Even before I discovered I was Hep C+. I went thru tx( peg-intron- ...
New studies in mice suggest that blood platelets can destroy deadly malaria parasites, but a single dose of aspirin may be ... Platelets are well known for their role in blood clotting and blood vessel repair. Previous studies have shown that platelets ... We believe that s because platelets bind to infected red blood cells and are taken out of circulation because of that, Foote ... New studies in mice suggest that blood platelets can destroy deadly malaria parasites, but a single dose of aspirin may be ...
Blood platelets are deceptively simple cells. They circulate in the form of flattened disks, dwarfed by the larger erythrocytes ... The Cytoskeleton of Human Blood Platelets. In: Harris J.R. (eds) Megakaryocytes, Platelets, Macrophages, and Eosinophils. Blood ... White, J. G., and Sauk, J. J., 1984, Microtubule coils in spread blood platelets, Blood 64: 470-478.PubMedGoogle Scholar ... Actin Filament Human Platelet Detergent Extraction Parallel Bundle Human Blood Platelet These keywords were added by machine ...
Changes in blood platelets triggered by COVID-19 could contribute to the onset of heart attacks, strokes, and other serious ... Tags: Aspirin, Blood, Blood Clot, Blood Pressure, Diabetes, Gene, Gene Expression, Genetic, Heart, Hematology, High Blood ... Manne, B.K., et al. (2020) Platelet Gene Expression and Function in COVID-19 Patients. Blood. ... Changes in blood platelets triggered by COVID-19 could contribute to the onset of heart attacks, strokes, and other serious ...
Blood group alters platelet binding kinetics to von Willebrand factor and consequently platelet function Eimear Dunne, Qin M. ... Blood 2019 :blood-2018-10-881557; doi: ... Blood 2019 :blood-2018-11-835371; doi: ... Megakaryocytes and platelets from a novel human adipose tissue-derived mesenchymal stem cell line Keiichi Tozawa, Yukako Ono- ...
... caused by low blood platelets. Have any of you ever ... ... Low Blood Platelets By kschmitz, January 25, 2005. in Related ... caused by low blood platelets. Have any of you ever had this diagnosis? Low platelets can be the result of an autoimmune ... Just wondering how the platelets and petechaia turned out -- still there? How low are your platelets? ... Blood? Same. Since that period I have ordered re-tests on all three types of tests, this time from different companies, and ...
What causes the increase? What is the danger of too many platelets? -- P.D. ... and my platelet count was 470,000. Three months ago it was 500,000. A week ago it was 650,000. ... Donohue: I had blood work done six months ago, ... TOO MANY PLATELETS CAN LEAD TO BLOOD PROBLEMS. DR. PAUL DONOHUE ... Polycythemia is the blood illness where bone marrow makes too many red and white blood cells and too many platelets. By now, ...
The American Red Cross urges eligible donors to end summer on a positive and potentially lifesaving note with a blood or ... but blood and platelet donors are still needed to help ensure blood products are available to meet hospital patient needs. ... Blood can be safely donated every 56 days and platelets can be given every seven days - up to 24 times a year. To make an ... Red Cross urges eligible donors to close out summer with a blood or platelet donation ...
Platelets are a part of the blood that helps the blood clot. ... shows if you have antibodies against platelets in your blood. ... This blood test shows if you have antibodies against platelets in your blood. Platelets are a part of the blood that helps the ... Abnormal results show that you have anti-platelet antibodies. Anti-platelet antibodies may appear in the blood due to any of ... Platelet and blood vessel disorders. In: Kliegman RM, St. Geme JW, Blum NJ, Shah SS, Tasker RC, Wilson KM, eds. Nelson Textbook ...
Emerging roles for platelets as immune and inflammatory cells. Blood. 2014;123(18):2759-2767. ... In this issue of Blood, Stefanini et al untangle the specific roles of RAP1A and RAP1B in platelet production, signal ... Genome-wide RNA-seq analysis of human and mouse platelet transcriptomes. Blood. 2011;118(14):e101-e111. ... RAPid signaling in platelets. Blood, 132(18), 1864-1865. Accessed November 14, 2018. ...
City of Hope strongly supports and values the uniqueness of all individuals and promotes a work environment where diversity is embraced. ...
... a change in diet can help build up blood platelets. An organic diet rich in nutrients can have a beneficial effect on someones ... What Is the Normal Blood Platelet Level?. A: In healthy adults, the normal blood platelet count is 150,000 to 400,000 platelets ... What Are High Blood Platelets a Sign Of?. A: Having too many platelets in the blood is a sign of either thrombocytosis or ... What Is the Easiest Way to Raise Blood Platelets?. A: The easiest way of increasing the level of blood platelets is by eating ...
BE A HERO THIS VALENTINES DAY Schedule an appointment to donate blood or platelets using the Red Cross Blood Donor App, ... POWER REDS AND PLATELETS NEEDED Eligible donors with types O, A negative and B negative blood are urged to make a Power Red ... Blood and platelet donors are heroes who answer the call to roll up a sleeve and help ensure an adequate supply is available ... Unlike whole blood, which can be safely donated every 56 days, platelets can be donated every seven days, up to 24 times a year ...
Transfusion of whole blood, which contains plasma and all blood cell types including red blood cells and platelets, or packed ... Thrombocytopenia refers to an abnormally low blood-concentration of platelets, which are blood cells that promote blood ... Dogs with blood platelet concentrations of less than 40,000 per microliter of blood are at risk for spontaneous bleeding. ... Low Blood Platelets) in Dogs. Treatment for thrombocytopenia depends on the underlying cause of the low platelet count. ...
Platelets allow blood to clot, so the main symptoms of low blood platelets include bruising, an inability to stop bleeding from ... Low blood platelets can be a dangerous condition. ... Platelets are cells in the blood that help the blood to clot, ... Low blood platelets can be a dangerous condition. Platelets allow blood to clot, so the main symptoms of low blood platelets ... There are several treatments available for people with low blood platelets. If your low blood platelet count is due to ITP, you ...
... the BMC wants blood banks to supply blood and its components such as platelets, plasma, judiciously. ... To avoid adverse effects in patients as a result of excessive and unnecessary blood transfusions, ... The civic body wants blood banks to monitor supply of blood platelets and other components and identify any over-prescription ... the BMC wants blood banks to supply blood and its components such as platelets, plasma, judiciously. mumbai Updated: May 22, ...
... and platelets. [M -C Shen; C -M Teng; Akikazu Takada;] ... "Blood Platelets"@en ;. schema:name "Blood platelets"@en ;. .. < ... Blood platelets. a schema:Intangible ;. schema:name "Blood platelets"@en ;. .. ...> # Blood Platelets. a schema:Intangible ;. rdfs ... schema:about blood_platelets> ; # Blood Platelets. schema: ...
In a healthy person, a large number of platelets are manufactured and stored in the body. ... Platelets are the component of blood that helps with clotting. ... Platelets are about a quarter of the size of red blood cells ... Platelets are the component of blood that helps with clotting. If an injury or blood loss occurs, platelets are released, and a ... Regular donors can give platelets every two weeks. Ask us about donating platelets at your next whole blood donation or give us ...
blood platelet synonyms, blood platelet pronunciation, blood platelet translation, English dictionary definition of blood ... n. any of the minute, nonnucleated cellular elements in mammalian blood essential for coagulation. Noun 1. blood platelet - ... blood platelet. Also found in: Thesaurus, Medical, Encyclopedia. blood platelet. n.. See platelet. ... blood platelet - tiny bits of protoplasm found in vertebrate blood; essential for blood clotting. platelet, thrombocyte ...
The blood platelet in transfusion therapy. [Tibor J Greenwalt; G A Jamieson; American National Red Cross.;] ... Blood platelets--Transfusion a schema:Intangible ;. schema:name "Blood platelets--Transfusion"@en ;. . ... Blood platelets--Transfusion a schema:Intangible ;. schema:name "Blood platelets--Transfusion"@en ;. . ... The blood platelet in transfusion therapy. Author:. Tibor J Greenwalt; G A Jamieson; American National Red Cross.. ...
... Platelet plug formation is a major step in physiological hemostasis, but also in ... Approximately 750 billion platelets circulate in human blood, constantly scanning the vasculature for damage of the endothelial ... followed by platelet activation, adhesion and spreading. During this stepwise processes platelets undergo dramatic shape ... Our main goal is to dissect the molecular and mechanical cues regulating platelet cytoskeleton dynamics and platelet motility ...
Research in the Laboratory for Hemostasis and Platelet Biology, led by Dr. Andrew Johnson, focuses on understanding genetic and ...
Platelets from all donor types are needed.Platelets have a very limited shelf life of five days and are delivered quickly to ... Patients undergoing bone marrow transplants, surgeries, chemotherapy, radiation or organ transplants often need platelets to ... Platelets are blood cells that help control bleeding. ... Platelets are blood cells that help control bleeding. Patients ... Platelet donors are eligible every two weeks. The collection process is longer than whole blood so you should be prepared to ...
... or give blood on board the Dana-Farber Cancer Institute/Brigham and Womens Hospital Blood Mobile, you are making a life-saving ... When you donate blood or platelets at the Kraft Family Blood Donor Center at Dana-Farber Cancer Institute and Brigham and ... All of the blood and platelets collected at the Kraft Center and on the Blood Mobile benefit patients at Dana-Farber and ... When you donate blood or platelets at the Kraft Family Blood Donor Center at Dana-Farber Cancer Institute and Brigham and ...
Platelets are really special, because thats what were using for trauma victims, someone who has just got of surgery or cancer ... It turns out the platelet donation process is a bit more time-consuming that the typical blood donation-it can take up to two ... He knows the location well, his donation of a pint of blood platelets brings his total contributions, to date, to nearly 50 ... Donors can specify whether theyre willing to give plasma, red blood cells or platelets. ...
Donating blood or platelets is safe and simple, and it can save lives. ... More than 44,000 blood donations are needed every day, according to the American Red Cross. ... How to donate blood or platelets. If youd like to donate blood or platelets, a good first step is to find your local Red Cross ... Why blood and platelet donations are needed. Unlike medicine, blood products cannot be made in a laboratory. But sometimes, ...
Re: low blood platelet you should for sure ask about itp i also had it when i was younger and low platelet count is the biggest ...
Seattle-based company Blood Cell Storage develop an innovative optical sensing device designed to measure the pH of platelet ... products without compromising blood storage bags. The florescence sensor integrated with a platelet storage bag allows quality ... monitoring without compromising the bag - a first in the blood platelet industry. ... Optical Sensing Device for Blood Platelets. Researchers at Pacific Northwest National Laboratory helped the Seattle-based ...
  • The American Red Cross urges eligible donors to end summer on a positive and potentially lifesaving note with a blood or platelet donation this Labor Day holiday. (
  • Since we first began our partnership in 2012, they have gone above and beyond to serve as champions for blood donation - from raising awareness and volunteering, to donating blood and platelets themselves. (
  • Those who come to donate through February 26, 2017 are eligible to receive a $5 gift card via email for making blood and platelet donation a priority this winter. (
  • POWER REDS AND PLATELETS NEEDED Eligible donors with types O, A negative and B negative blood are urged to make a Power Red donation, where available. (
  • Power Red donors give a concentrated dose of red blood cells during a single donation, allowing them to maximize their impact. (
  • Platelets must be transfused within five days of donation, so it's important that eligible platelet donors give as often as possible. (
  • Once a campaign is created, invite colleagues, friends and family to pledge their support by making an appointment to donate at a blood drive or donation center convenient for them. (
  • Those who are not eligible to give blood, do not have a Red Cross blood donation opportunity nearby or simply want to do more can support a SleevesUp campaign by making a financial donation. (
  • The platelet donation process follows the same steps as whole blood donation registration, screening, donation and after-care. (
  • Ask us about donating platelets at your next whole blood donation or give us a call at 1 888 2 DONATE (1-888-236-6283). (
  • How long does a platelet donation take? (
  • The plateletpheresis process is usually about 75 to 100 minutes for a large-volume donation and about 50 to 75 minutes for a single-unit donation, depending on your platelet count. (
  • Large-volume donations collect two times more platelets than a single donation. (
  • One large volume donation makes twice as much transfusable platelet units. (
  • Since our bodies manufacture and store extra platelets, the platelets collected during your donation is replaced almost immediately. (
  • If you do wish to donate both blood and platelets, you need to wait 56 days after either type of donation before making the other. (
  • You're welcome to try different donation programs to see which you like best: blood, plasma or platelets. (
  • If you are thinking of making your first platelet donation or want to book an appointment, call your local donor centre. (
  • Is it possible to draw only platelets from a donation? (
  • As with a whole blood donation, we insert a needle into your arm and draw blood from your body. (
  • Dr Shahinaz clarified that blood platelet donation is similar to blood donation but it takes longer time. (
  • You can Make the Most of Your Donation by becoming a platelet donor. (
  • When you give platelets, you have the satisfaction of knowing your donation will make a difference in someone's life immediately. (
  • But first, he'll be going to the local Red Cross blood donation center. (
  • He knows the location well, his donation of a pint of blood platelets brings his total contributions, to date, to nearly 50 gallons. (
  • It turns out the platelet donation process is a bit more time-consuming that the typical blood donation-it can take up to two hours-but donations can be made more often (weekly) as opposed to regular blood cells, which can be donated every 42 days. (
  • And donors can certainly refer to that when they come in, but most of the time, people just say 'platelet donation. (
  • Blood donation drives are slated at locations across the Treasure Valley throughout the summer. (
  • Giving a whole-blood donation usually takes about 10 minutes. (
  • This process is called apheresis and is slightly different from giving a whole-blood donation. (
  • During the platelet donation, blood is removed from one arm, and then a centrifuge separates out the platelets. (
  • More platelets are collected this way than with whole-blood donation. (
  • Platelet donation takes 2 hours and can have mild side effects like chills or tingling. (
  • Talk with your doctor's office or local blood donation center to find out more. (
  • Why do blood donation centers bother with platelets? (
  • I gave a 2-hour platelet donation today and the whole process seemed inefficient. (
  • Is it really much more efficient to get platelets this way rather than separating them out of a whole blood donation that takes much less time and potentially less of their employees time? (
  • There are only a small number of platelets in a normal blood donation, they can extract up to 4-8 times the amount of platelets the way they did with you today. (
  • Also patients who need platelets often only need platelets, not a whole blood transfusion (this is common for cancer patients since chemo is so hard on platelets), so you donating just platelets and getting back the rest means they don't have to post-process your donation to remove components. (
  • Yes the donation takes longer, but I've never had to wait in line so clearly most blood centers are able to handle multiple types of traffic without too much trouble. (
  • When I donate, I can usually give a triple, so someone who needs 3 units of platelets can use my whole donation, and have only one chance at that incompatibility. (
  • Gotcha - so they get many more platelets per donation, and individuals who give platelets regularly can give much more often than whole blood, so even if they have fewer donors they're able to get more platelets this way than from whole blood. (
  • TUCSON, AZ (Tucson News Now) - Ongoing severe winter weather has more than doubled the number of canceled American Red Cross blood drives and the resulting blood and platelet donation shortfall since earlier this month. (
  • Below is a list of upcoming blood donation opportunities in southern Arizona through Thursday, Feb. 15. (
  • The Red Cross checks your blood pressure before every donation! (
  • If you are a platelet donor, you may be told to increase your calcium intake a day or two prior to your donation. (
  • One platelet donation can contain enough platelets for three therapeutic doses . (
  • Are you eligible for blood donation? (
  • Learn how blood donations help, what to expect, and how to get ready for your first blood donation. (
  • New to blood donation? (
  • Enter your information to learn the truth about some blood donation myths and how you can help patients. (
  • Jordan was willing to sacrifice the extra time needed to give platelets, but a whole blood donation, when WHNT News 19's Chris Davis went through the process on Wednesday, took just under 30 minutes. (
  • Please visit for complete information about donor eligibility and the donation process for blood or platelets. (
  • Below, explore the blood and platelet donation facts and figures to learn how you can give the gift of life to pediatric patients at Children's National. (
  • While the American Red Cross worked nonstop to support those affected by Hurricane Dorian, the storm forced blood donation centers and blood drives to be closed. (
  • Platelets -- a key clotting component of blood often needed by cancer patients, burn victims and bone marrow recipients -- must be transfused within five days of donation. (
  • In order to donate covalescent plasma you must qualify for blood donation. (
  • Clark County Blood Donation Center, 1805 E. 8th St.Sellersburg3/16/2015: 3 p.m. - 7 p.m. (
  • East End Louisville Blood Donation Center, 291 N. Hubbards Lane3/10/2015: 11 a.m. - 6:30 p.m. (
  • Downtown Louisville Blood Donation Center, 520 E. Chestnut St.3/10/2015: 3 p.m. - 7 p.m., St. Edward, 9608 Sue Helen Drive3/10/2015: 4 p.m. - 7 p.m. (
  • Downtown Louisville Blood Donation Center, 520 E. Chestnut St.3/12/2015: 12 p.m. - 7 p.m. (
  • Downtown Louisville Blood Donation Center, 520 E. Chestnut St.3/14/2015: 8 a.m. - 1 p.m., St. Athanasius, 5915 Outer Loop3/14/2015: 9 a.m. - 1 p.m. (
  • East End Louisville Blood Donation Center, 291 N. Hubbards Lane3/14/2015: 10 a.m. - 2 p.m., St. Marks Episcopal Church, 2822 Frankfort Ave.3/15/2015: 8:30 a.m. - 1:30 p.m. (
  • We have an urgent need for blood and platelet donations during the holidays, and blood donation is a great way for blood donors to spread Christmas cheer. (
  • This year on Feb. 26, we honor Dr. Reiss and her legacy through the first-ever Texas Bone Marrow, Blood, and Organ Donation Registry Day, established through the work of State Rep. Gene Wu and passed into law last year. (
  • What type of blood donation works for you? (
  • Whole blood, platelets, red blood cells or plasma - you've got options when it comes to saving lives, and each donation has its benefits. (
  • Visit us today to make your next life-saving blood and platelet donation. (
  • In order for your donation to go to Andrew, you must donate blood in the Blood Donor Room at Memorial Sloane Kettering. (
  • Mass Appeal) - Blood platelet donors are known as "cancer kickers" because this special type of donation is used to support patients through cancer treatment. (
  • You can give the gift of life this week at the 22News blood drive happening at the American Red Cross Blood Donation Center, 150 Brookdale Drive in Springfield. (
  • Our platelet donation clinics are in Dublin and Cork. (
  • Yet many people have never heard of platelet donation. (
  • Find out more about the platelet donation process by watching the video below featuring Kyran O'Brien, one of our platelet donors, and the journey platelets take from the clinic in the National Blood Centre, through the labs to treat a young patient in Temple Street Children's University Hospital. (
  • Saving lives is now a regular part of Ralph "Rocco" Russo's life, after an incredible 15 years of platelet donation at Dana-Farber Cancer Institute. (
  • Platelets and plasma can be taken from a whole blood donation too. (
  • But one donation of platelets by apheresis provides the same amount of platelets as 8 to 12 whole blood donations combined. (
  • The record cold exposed a key problem underlying our nation's blood donation system. (
  • The current blood donation system is vulnerable to disruption. (
  • Has anyone who uses AAS ever tried the platelet/whole blood donation where they hook you up to the machine and extract the red blood cells, and return the plasma in your body? (
  • We are urging all eligible donors to do their part to support the community blood supply by calling to schedule a donation appointment this week to help overcome the loss of collections due to the inclement weather," Brown added. (
  • Interested donors can schedule an appointment by calling (800) GIVE LIFE, or by going online to Pacific Northwest Regional Blood Services , where they can also find schedule of convenient donation locations. (
  • Platelet transfusions also may be an option for all of the causes of low platelet count. (
  • To avoid adverse effects in patients as a result of excessive and unnecessary blood transfusions, the BMC wants blood banks to supply blood and its components such as platelets, plasma, judiciously. (
  • The Brihanmumbai Municipal Corporation (BMC) has called for a meeting next week with blood banks operating in Mumbai to discuss the 'unnecessary platelet transfusions' in patients, especially those suffering from dengue. (
  • Platelet transfusions are lifesaving but can be fatal also as it can cause bleeding," said a senior doctor who is a part of the committee. (
  • The doctor said that there had been a case where a 60-year-old man admitted at a private hospital with dengue died, after he was given multiple platelet transfusions even when his platelets were a healthy 70,000 units. (
  • Transfusion-related acute lung injury is one of the most common complications of blood transfusions. (
  • Doctors have to be extremely cautious while perfor ming blood transfusions in pregnant women," said Dr Rekha Daver, head of gynaecology department, JJ Hospital, Byculla. (
  • Patients who have low platelets or platelets that don't function properly (e.g. cancer patients undergoing chemotherapy) need platelet transfusions as part of their treatment. (
  • Patients who receive multiple platelet transfusions sometimes produce antibodies that fight the donated platelets and prevent them from performing their clotting function. (
  • People with cancer do not often need transfusions of this part of blood. (
  • When cancer or its treatment causes low RBCs, called anemia , whole blood transfusions are used to replace the RBCs. (
  • Whole blood transfusions can be used to replace blood lost during surgeries, too. (
  • Ryan Halliday, the godson of retired FDNY Firefighter Tom Carroll of Engine 219, is 22 years old and needs blood and platelet transfusions. (
  • Our study is the first one to show that platelet transfusions are frequently administered to patients with ITP, HIT and TTP, and that they're associated with higher odds of arterial blood clots and mortality in TTP and HIT. (
  • When a panel of experts convened by the AABB -- formerly known as the American Association of Blood Banks -- issued guidelines for platelet transfusions in November 2014, it made no recommendation on treatments for ITP, TTP and HIT. (
  • Our analysis found no significantly increased risks from platelet transfusions in ITP," Goel says. (
  • In HIT, platelet transfusions increased the risk of bleeding fivefold and the risk of an arterial clot more than threefold. (
  • The researchers were surprised to find that one in 10 TTP patients and one in 13 HIT patients got platelet transfusions, in spite of some practitioners' concerns about the risks. (
  • Tobian and colleagues believe that for patients with HIT and TTP, platelet transfusions should be reserved "only for severe, life-threatening bleeding refractory to other therapies or major surgery. (
  • They need transfusions of healthy platelets to control their bleeding. (
  • As new treatments develop, more patients are being successfully treated with platelets which mean there is a growing demand for platelet transfusions. (
  • Management of symptoms related to cancer and cancer treatments may require blood transfusions. (
  • For patients who need platelet transfusions, platelets must first be extracted from plasma. (
  • Blood transfusions can have some bad side affects. (
  • In our OOU unfortunately I see a lot of blood transfusion (I say unfortunately because I tend to faint at the sight of blood) every day there are at least 2 or 3 transfusions going on. (
  • Steve has had many blood transfusions. (
  • Platelet transfusions came into medical use in the 1950s and 1960s. (
  • International guidelines recommend that platelets transfusions are given to people with reversible bone marrow failure to reduce the risk of spontaneous bleeding when the platelet count is less than 10 x 109/L. If the person is well using a higher platelet count threshold does not reduce the risk of bleeding further. (
  • A review in people with blood cancers receiving intensive chemotherapy or a stem cell transplant found that overall giving platelet transfusions when the platelet count is less than 10 x 109/L reduced the number of bleeding events and days with significant bleeding. (
  • Despite prophylactic platelet transfusions, people with blood cancers often bleed, and other risk factors for bleeding such as inflammation and duration of thrombocytopenia should be considered. (
  • There is little evidence for the use of preventive platelet transfusions in people with chronic bone marrow failure, such as myelodysplasia or aplastic anemia. (
  • Multiple guidelines recommend prophylactic platelet transfusions are not used routinely in people with chronic bone marrow failure, and instead an individualised approach should be taken. (
  • Several studies have now assessed the benefit of using preventive platelet transfusions in adults with dengue who have profound thrombocytopenia (platelet count (
  • Two reviews in people with blood cancers receiving intensive chemotherapy or a stem cell transplant found that overall giving platelet transfusions when the platelet count is less than 10 x 109/L compared to giving platelet transfusions when the platelet count is less than 20 or 30 x 109/L had no effect on the risk of bleeding. (
  • This review found no difference in the number of people who had clinically significant bleeding between platelet transfusions that contained a small number of platelets (low dose - 1.1 x 1011/m2) and those that contained an intermediate number of platelets (intermediate dose - 2.2 x 1011/m2). (
  • In people with a low platelet count, prophylactic platelet transfusions do not need to be given prior to procedures that have a low risk of causing bleeding. (
  • The evidence is very uncertain about the effect of platelet transfusions prior to surgery for people with a low platelet count on the all-cause mortality, the number of participants with bleeding events after surgery, serious surgery-related or transfusion-related adverse events. (
  • Clinicians should collaborate with hospital transfusion services and blood collection center personnel, and, when necessary, notify health departments, to manage suspected infections in blood donors and patients. (
  • In early August, the Red Cross issued an urgent call for blood donors with types O negative, B negative and A negative blood to donate to help avert an emergency situation. (
  • Thousands of people have answered the call to donate in recent weeks, but blood and platelet donors are still needed to help ensure blood products are available to meet hospital patient needs. (
  • Eligible donors are encouraged to make an appointment to give blood or platelets this Labor Day weekend. (
  • Blood donors with all types, especially O negative, B negative and A negative, are urged to give. (
  • Platelet donors and those with type AB blood are also continually needed. (
  • Blood and platelet donors are heroes who answer the call to roll up a sleeve and help ensure an adequate supply is available for patients in need. (
  • Thankfully, blood donors came through for Jacqueline and she had multiple successful surgeries. (
  • Jacqueline shared a special drawing, complete with red hearts, to express her ongoing gratitude to blood donors. (
  • A single platelet treatment would require six to eight whole blood donors instead of just one plateletpheresis donor. (
  • Regular donors can give platelets every two weeks. (
  • New platelet donors are encouraged to donate once a month. (
  • Summary: Dubai hopes to keep special blood platelet supply running smoothly even after encouraging paid donors to become unpaid volunteers by 2020, said a senior health official. (
  • Platelet donors are eligible every two weeks. (
  • New England Patriots alum Joe Andruzzi visited the Kraft Family Blood Donor Center at Dana-Farber Cancer Institute and Brigham and Women's Hospital to greet blood and platelet donors, as well as pediatric patients at Dana-Farber/Boston Children's Cancer and Blood Disorders Center. (
  • Donors can specify whether they're willing to give plasma, red blood cells or platelets. (
  • Replenishing blood lost from an injury or chemotherapy requires blood from healthy donors. (
  • I'm not talking about inefficiency for for the donor, though it strikes me that they probably have fewer regular donors for platelets since it takes quite a while. (
  • Where as they'd have to get 16 individual donors in who would have to wait 56 days between donations to get the same number of platelets from whole blood. (
  • If that same quantity of platelets came from whole blood, it could have been the product of 20+ donors, so the chance of incompatibility is that much greater. (
  • Additionally, as wwax notes, even though they may have a smaller pool of donors, those donors can donate more frequently (7 days between donations/24 donations per year is the Red Cross limit), so the total amount collected can be much higher than from whole blood. (
  • The American Red Cross considers the situation critical and has reissued an urgent call for blood and platelet donors. (
  • The American Red Cross has a critical shortage of type O blood and urges type O donors to give now to ensure blood is available for patients facing trauma and other life-threatening situations. (
  • We're now calling out for more donors to help us meet that extra need that we're helping to fulfill along with the other community blood centers across the country," says Franchois. (
  • An adult dose of platelets derived from whole blood is obtained from a pool of buffy coats from four ABO identical donors and is resuspended in a nutrient additive solution to produce a pooled platelet component. (
  • Being able to count on volunteer blood donors at all times is especially important because blood has a limited shelf life. (
  • Volunteer donors ensure that there will be a sufficient supply of blood for the patients who need it, whenever they need it. (
  • Volunteer donors are the only source of platelets for these patients. (
  • Being able to count on volunteer platelet donors at all times is especially important because platelets have a shelf life of just five days. (
  • Volunteer donors ensure that there will always be a sufficient supply of platelets for patients in need. (
  • Right now, we're encouraging eligible donors to come out and give to prevent a shortage," said Red Cross spokesman Ben Corey of the Peoria-based Red Cross Heart of Blood Services America region, which oversees West-Central Illinois and Northeast Missouri. (
  • Hospital patients continue to need lifesaving blood this summer, and they're relying on the generosity of volunteer donors to give them hope in the days and weeks ahead," Heiden said. (
  • After round after round of snow, freezing rain and arctic cold in many parts of the country, the American Red Cross has an urgent need for eligible blood and platelet donors to donate to help restock its supplies.March storms forced the cancellation of more than 200 blood drives, resulting in nearly 7,000 uncollected blood and platelet donations. (
  • The Red Cross is asking donors of all blood types to make and keep appointments to help restock its supplies. (
  • Platelet donors, as well as blood donors with the most in-demand blood types - O negative, A negative and B negative - are particularly encouraged to help replenish the blood supply.Platelets help prevent massive blood loss and are a vital part of cancer and organ transplant treatments. (
  • Eligible donors with types O negative, A negative and B negative blood are encouraged to donate double red cells where available. (
  • We encourage many of our donors to give platelets through an automated system. (
  • Cole was one of several organ, stem cell and blood donors and recipients honored in the 2019 Lone Star Circle of Life Bike Tour. (
  • Platelet donors make an immediate impact. (
  • The goal of the UCLA Blood & Platelet Center is to provide patients with safe blood collected from volunteer donors. (
  • Due to the rising number of cancer diagnosis in the country, there is always a need for platelet donors. (
  • There are just 2,400 Irish platelet donors and we are looking for new donors to join the panel. (
  • It relies exclusively on volunteer donors to provide blood for transfusion. (
  • In addition to icy, cold weather that has kept many donors from their appointments, colds and flu, and busy schedules after the return from the holidays are also driving down the blood collection rates. (
  • The actual risk of transfusion-associated sepsis is likely higher, as infections due to contaminated blood products are under-reported. (
  • It is critical that clinicians be aware of the problem of bacterial contamination of blood products, particularly platelets, and consider the possibility of bacterial contamination when investigating febrile transfusion reactions. (
  • If bacterial contamination of a component is suspected, the transfusion should be stopped immediately, the unit saved for further testing, and blood cultures should be obtained from the patient. (
  • Clinician is contacted by the blood collection or transfusion service with information involving a blood or blood component with bacterial contamination. (
  • A blood or blood component recipient has signs or symptoms consistent with post-transfusion bacteremia. (
  • It is difficult to increase platelet numbers adequately by transfusion, and transfused platelets do not last very long - a few days at most. (
  • Transfusion of whole blood, which contains plasma and all blood cell types including red blood cells and platelets, or packed red cells, which contains red blood cells without plasma, may be necessary in the event of life-threatening hemorrhage or if your dog is anemic from previous blood loss. (
  • Low platelets due to chronic liver disease is generally treated by a platelet transfusion. (
  • The blood was then used in a December 2007 transfusion. (
  • Purely hypothetically, it's conceivable that a transfusion of platelets could be administered to restore balance and calm everything down if there are signs of a growth of pathological blood vessels if the child is found to have low levels of platelets,' Ann Hellstrom says. (
  • This time of year brings holiday cheer to many people across the nation, but for hospitals and blood donor centers, such as City of Hope's Michael Amini Transfusion Medicine Center , it's the start of a tough season. (
  • In cases of uncontrolled bleeding, death can happen in minutes or hours if victims are not treated quickly with a transfusion to replace platelets and other blood cells. (
  • Blood platelets ready for transfusion during surgery. (
  • The study authors recommend that for these rare disorders, doctors should administer the treatment, a platelet transfusion, only in exceptional circumstances. (
  • But in TTP, a platelet transfusion increased the odds of a potentially lethal arterial blood clot more than fivefold and doubled the odds of a heart attack. (
  • In TTP, the odds of dying in the hospital doubled when the patient was given a platelet transfusion. (
  • In HIT, the odds of dying were five times greater with a platelet transfusion. (
  • In some cases, Tobian says, doctors may not know the patient has a platelet disorder until they see the potentially deadly reaction to the transfusion. (
  • Nationwide, the Red Cross needs to accumulate 15,000 blood donations every day to meet the needs of patients at about 2,700 hospitals and transfusion centers across the country. (
  • The Irish Blood Transfusion Service provides life-saving platelets to all of the hospitals in Ireland. (
  • A platelet transfusion can be the difference between life and death for leukaemia patients and people undergoing chemotherapy. (
  • A transfusion is the administration of blood or blood components through a catheter, a tube that enters the body through an intravenous (IV) needle, central venous catheter (CVC), or peripherally-inserted central catheter (PICC). (
  • A transfusion can include all or any one of the blood components, and may come from a donor or may have been harvested from the patient prior to therapy. (
  • Before a transfusion can be given, results of blood studies must first be analyzed to help determine which blood component the patient will need. (
  • has anyone here had a blood transfusion? (
  • not sure why blood transfusion is needed. (
  • If you are on hormonal, biological (like Avastin or Erbitux) or radiotherapy you cannot get the proscrit shot and require a blood transfusion. (
  • i didn't have any problems with the platelet transfusion and I know just one,a that was next to me getting a transfusion told the nurse that about half way through he was starting to feel much better and felt more energy(he had severe anemia). (
  • Platelet transfusion, also known as platelet concentrate, is used to prevent or treat bleeding in people with either a low platelet count or poor platelet function. (
  • Preventive transfusion is often done in those with platelet levels of less than 10 x 109/L. In those who are bleeding transfusion is usually carried out at less than 50 x 109/L. Blood group matching (ABO, RhD) is typically recommended before platelets are given. (
  • Higher platelet transfusion thresholds have been used in premature neonates, but this has been based on limited evidence. (
  • A review in people with blood cancers compared different platelet transfusion doses. (
  • This study's authors suggested that a high-dose platelet transfusion strategy may lead to a higher rate of transfusion-related adverse events. (
  • Thrombocytopenia caused by platelet destruction, hypersplenism, or hemodilution. (
  • Thrombocytopenia refers to an abnormally low blood-concentration of platelets, which are blood cells that promote blood clotting after injury to the lining of the blood vessels. (
  • The severity of bleeding associated with thrombocytopenia depends on how low the platelet numbers fall. (
  • Treatment for thrombocytopenia depends on the underlying cause of the low platelet count. (
  • People who have a low blood platelet count, or thrombocytopenia, cannot create platelets as most of our bodies do. (
  • Biopharmaceutical company Ligand Pharmaceuticals Incorporated's ( NasdaqGM:LGND) partner GlaxoSmithKline (NYSE:GSK) on Monday jointly announced the receipt of approval for PROMACTA for the treatment of thrombocytopenia (low blood platelet counts) in patients with chronic hepatitis C to allow them to initiate and maintain interferon-based therapy. (
  • People with cancer may develop low platelets, or thrombocytopenia , when the body's bone marrow is damaged from some kinds of chemotherapy or from some types of leukemia or lymphoma. (
  • An abnormally small number of platelets, or thrombocytopenia, result from conditions that either impairs production, increase destruction, or cause sequestration of platelets. (
  • The regulatory body said: "One plausible explanation for the combination of blood clots and low blood platelets is an immune response, leading to a condition similar to one seen sometimes in patients treated with heparin called heparin induced thrombocytopenia, HIT. (
  • The Johns Hopkins-led study, published Jan. 14, 2015 in Blood , the journal of the American Society of Hematology, is the first nationwide review of nearly 100,000 combined hospital admissions for three rare blood cell disorders: thrombotic thrombocytopenic purpura (TTP), heparin-induced thrombocytopenia (HIT) and immune thrombocytopenic purpura (ITP). (
  • See also Overview of Platelet Disorders and Overview of Thrombocytopenia . (
  • Doctors first do a complete blood count (CBC) to measure the number of platelets and see whether the person's symptoms are caused by a low number of platelets ( thrombocytopenia ). (
  • Platelet aggregation response in immune thrombocytopenia patients treated with romiplostim. (
  • As a consequence of that mutation, the mouse produced just one-tenth the normal amount of platelets. (
  • In one case cited by the FDA, CBCF staff did not have a sufficient amount of platelets in a bag and filled the remainder with platelets that had been sitting in a biohazardous waste bin for more than 21 hours -- and had not been stored at proper temperature. (
  • Only a small amount of platelets make up plasma. (
  • In laboratory studies, they studied platelet aggregation, an important component of blood clot formation, and observed COVID-19 platelets aggregated more readily. (
  • In this traditional role, platelets patrol the cardiovascular system as specialized cells that rapidly mount a response to stop bleeding via aggregation at the site of vascular damage. (
  • These pathways converge at the guanine exchange factor CALDAG-GEFI to activate the GTPase RAP1 (see figure panel A). 4 RAP1 activation leads to rapid platelet adhesion and aggregation mediated by integrins. (
  • Sphingosine 1-Phosphate Produced by Sphingosine Kinase 2 Intrinsically Controls Platelet Aggregation In Vitro and In Vivo. (
  • According to Becker, three of the seven genes had been previously reported as having some role in platelet aggregation, but "it was not until now that we put together all the major pieces of the genetic puzzle that will help us understand why some people's blood is more or less prone to clot than others and how this translates into promoting healing and stalling disease progression. (
  • We employed whole blood platelet aggregation analysis based on impedance as well as determination of ATP release from platelet granules detected by a chemiluminescence method. (
  • Platelet aggregation to low-dose ristocetin revealed an exaggerated response (20.9 ± 18.7 ohms, reference range: 0-5 ohms). (
  • however, unpolymerized methacrylate liposomes markedly inhibited ADP induced platelet aggregation. (
  • Platelet-activating factor, also known as PAF, PAF-acether or AGEPC (acetyl-glyceryl-ether-phosphorylcholine), is a potent phospholipid activator and mediator of many leukocyte functions, platelet aggregation and degranulation, inflammation, and anaphylaxis. (
  • These developments led to the finding that macrophages produce PAF and that macrophages play an important function in aggregation of platelets and liberation of their inflammatory and vasoactive substances. (
  • We found that the doctors were transfusing 20 to 30 units of platelets in a single patient. (
  • It hopes to get 800 units of blood and 560 units of platelets (that's equal to 400 people donating blood and approximately 190 people donating platelets). (
  • Trauma victims, anyone undergoing surgery, women giving birth, and others can require units of platelets to live. (
  • But with 7,000 units of platelets needed every day, the supply can't keep up with demand. (
  • Most hospitals have a limited supply of platelets due to their short shelf life. (
  • It's important to have a steady supply of platelets on hand," Corey said. (
  • We aren't alone in trying to improve the supply of platelets. (
  • Hospitals and blood banks are adopting new measures to improve the safety of donated platelets-the tiny cells that make blood clot and heal injuries but that also present the No. 1 infection risk in the U.S. blood supply. (
  • But with platelets, I believe, the worry is more about bleeding and how fast your blood will clot. (
  • If we can figure out how COVID-19 is interacting with megakaryocytes or platelets, then we might be able to block that interaction and reduce someone's risk of developing a blood clot. (
  • Platelets are a part of the blood that helps the blood clot. (
  • Platelets allow blood to clot, so the main symptoms of low blood platelets include bruising, an inability to stop bleeding from a wound, nosebleeds and skin conditions called purpura and petechiae. (
  • Platelets are cells in the blood that help the blood to clot, thereby healing wounds and preventing the loss of too much blood. (
  • If an injury or blood loss occurs, platelets are released, and a person's blood begins to clot to prevent excessive bleeding. (
  • There's little that first responders can do to help broken blood vessels clot. (
  • What if EMS workers carried synthetic platelets -- the blood's clotting agent - that they could inject to help the victims' blood clot, increasing the likelihood of them reaching a hospital alive? (
  • The peptide coating allows the nanoparticles to stick to a wound and recruit nearby platelets - both natural and artificial - to help form a clot, Pawlowski explained. (
  • Covid vaccine: EMA shares 'plausible explanation' for blood clot links - are you at risk? (
  • The European health body also proposed a "plausible" explanation for the blood clot link. (
  • Johnson & Johnson is the latest pharmaceutical giant to find itself at the centre of the blood clot controversy, after reports that linked its vaccine to blood clots prompted the EMA to release a statement yesterday. (
  • What does it feel like when you have a blood clot? (
  • The finding is curious because platelets (thrombocytes) are colourless blood cells that help blood clot. (
  • They allow blood to clot, helping wounds to heal. (
  • Cancer patients, people who have sustained trauma, babies in the neonatal intensive care unit, and many other critically ill patients are at serious risk because their blood does not clot properly. (
  • Considering taking medication to treat blood clot prevention with heparin-induced decreased platelet count? (
  • Below is a list of common medications used to treat or reduce the symptoms of blood clot prevention with heparin-induced decreased platelet count. (
  • Platelets are cells that are made in the bone marrow and circulate in the bloodstream and help blood clot . (
  • These tests measure how long it takes blood to clot. (
  • However, the fact that platelets play such a central role in blood clotting does mean that there is a higher risk of clot formation in thrombocytosis. (
  • With the activation of various tissue factors and the laying down of the tougher fibrin strands along with more platelets and blood cells, a blood clot is formed. (
  • These drugs inhibit the normal function of platelets to reduce the chance of clot formation. (
  • He's saved upwards of 600 lives, literally everytime he gives," said Dr. John Luckey, medical director of the Kraft Family Donor Center at Dana-Farber.Platelets help blood clot and are often wiped out during chemotherapy. (
  • Platelets help blood clot and are often wiped out during chemotherapy. (
  • Platelets perform a crucial function in blood, including helping blood to clot, which helps us heal wounds. (
  • Platelet and blood vessel disorders. (
  • RMS due to blood protein or platelet defects may come about through either of two mechanisms: (1) disorders associated with a hemorrhagic tendency or (2) defects associated with a thrombotic tendency. (
  • Heparin is a blood thinner given to people with clotting disorders. (
  • People hospitalized with certain rare blood cell disorders frequently receive a treatment that is associated with a two- to fivefold increase in death, according to a new study that reviewed hospital records nationwide. (
  • All three conditions are immune system disorders marked by low levels of the colorless blood cells called platelets that help seal up damaged blood vessels. (
  • Follow the links below to find trusted information about blood platelet disorders. (
  • There are a number of other rare inherited disorders that affect platelets, including Glanzmann disease, Wiskott-Aldrich syndrome , Chédiak-Higashi syndrome, and Bernard-Soulier syndrome. (
  • People with inherited disorders of platelet dysfunction may have a lifelong history of easy bruising or excessive bleeding after minor injuries or minor surgery such as dental extractions. (
  • Other symptoms of platelet disorders include tiny red dots (petechiae) on the skin and bruising after minor injuries. (
  • Doctors suspect an inherited cause if symptoms begin early in life in people who do not have any other disorders or do not take any drugs that cause platelet dysfunction. (
  • Blood Platelet Disorders" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (
  • Disorders caused by abnormalities in platelet count or function. (
  • This graph shows the total number of publications written about "Blood Platelet Disorders" by people in Harvard Catalyst Profiles by year, and whether "Blood Platelet Disorders" was a major or minor topic of these publication. (
  • Below are the most recent publications written about "Blood Platelet Disorders" by people in Profiles. (
  • López JA, Berliner N. Introduction to a series of reviews on clinical platelet disorders. (
  • Mumford AD, Frelinger AL, Gachet C, Gresele P, Noris P, Harrison P, Mezzano D. A review of platelet secretion assays for the diagnosis of inherited platelet secretion disorders. (
  • One due to bone marrow disorders that results in an overproduction of many blood components, including platelets. (
  • Body-wide abnormalities in coagulation (disseminated intravascular coagulation) can result in massive consumption of platelets. (
  • Dietary supplements (Vitamin e and fish oil) known to affect platelet function will be assessed and patients on those will be asked to discontinue these. (
  • Participants with also be asked to not eat foods known to affect platelet function (coffee, chocolate, grapes, and alcohol) 48 hours prior to sample collection on visit 1. (
  • The most common drugs that affect platelet function are aspirin and other nonsteroidal anti-inflammatory drugs (NSAIDs), along with antiplatelet drugs such as clopidogrel and similar drugs that are used to prevent strokes and heart attacks. (
  • Diseases that can affect platelet function include cirrhosis , multiple myeloma , kidney disease , and systemic lupus erythematosus (lupus). (
  • Yes, tx does effect all my blood counts. (
  • Only five percent of females and 20 percent of males with normal platelet counts died. (
  • For example, low platelet counts are often seen in malaria patients in the early stages of infection. (
  • Rap1a-mKO and Rap1b-mKO had normal platelet counts. (
  • ITP, or immune thrombocytopenic purpura, is the leading cause of low blood platelet counts. (
  • The treatment for hepatitis C often causes low blood platelet counts, as does chemotherapy. (
  • The patients have been diagnosed with persistent or chronic ITP, and have blood platelet counts consistently below 30,000/uL of blood. (
  • For 75% of patients with normal blood platelet counts, time off chemotherapy was associated with improved quality of life. (
  • 1. A new fixing solution is described, which preserves the platelets and prevents contact hemolysis of the erythrocytes, so that counts of both corpuscles may be made in the same preparation. (
  • 2. Comparative counts of platelets in arteries and veins show that arterial blood contains a larger number of platelets than venous blood. (
  • As a result my platelet counts are always high (currently 571. (
  • In a group of mice that received heart transplants, those with low platelet counts had heightened immune responses to the transplant, producing an unusually high number of immune cells called T helper 17 cells or Th17 cells. (
  • They may also be needed by patients undergoing major surgery, burns patients, accident victims who have had extensive injury and new born babies who are born with low platelet counts. (
  • Low platelet counts develop when platelet-producing bone marrow cells are damaged by chemotherapy or radiation therapy. (
  • Certain cancers, such as leukemia, can also cause low platelet counts. (
  • ENGLAND: Blood platelet counts at the higher end of normal suggest a high risk of cancer in men aged 60 or over, and should be investigated, according to new University of Exeter research. (
  • Using newly developed Romanowsky polychrome stains, he was able to follow the origin of platelets from fragmentation of megakaryocytes (Wright, 1910). (
  • In theory, inflammation caused by COVID-19 could affect megakaryocytes, the cells that produce platelets. (
  • As a result, critical genetic alterations are passed down from megakaryocytes to the platelets, which, in turn, make them hyperactive. (
  • In contrast, Rap1a/b-mKO mice had macrothrombocytopenia due to reduced proplatelet formation demonstrating that RAP1A and RAP1B have overlapping roles in megakaryocytes (see figure panel B). More importantly, this unexpected contribution of RAP1 signaling in megakaryocytes during platelet production opens several lines of investigation for future studies. (
  • Budding of the megakaryocytes forms cell fragments known as platelets. (
  • Platelets are made by large cells called megakaryocytes that live in bone marrow. (
  • The researchers found that inflammatory proteins produced during infection significantly alter the function of platelets, making them 'hyperactive' and more prone to form dangerous and potentially deadly blood clots. (
  • We found that inflammation and systemic changes, due to the infection, are influencing how platelets function, leading them to aggregate faster, which could explain why we are seeing increased numbers of blood clots in COVID patients. (
  • Dear P.D.: Platelets are the blood cells that form clots. (
  • Patients with thrombocytosis often develop clots in healthy blood vessels, and the clots block blood flow. (
  • Our results give us a clear set of new molecular targets, the proteins produced from these genes, to develop tests that could help us identify people more at risk for blood clots and for whom certain blood-thinning drugs may work best or not," says co-senior study investigator and cardiologist Lewis Becker, M.D. (
  • Anticoagulants or "blood thinners" are used to treat or prevent blood clots in the legs, lungs, or other parts of the body, and to prevent strokes. (
  • THE EUROPEAN Medical Agency (EMA) recommended that blood clots should be listed as a "very rare" side effect of the Johnson & Johnson vaccine in a statement on Tuesday. (
  • The European health body also put forward a "plausible explanation" for the cause of the blood clots. (
  • Therefore a low platelet count should in theory cause prolonged bleeding not blood clots. (
  • TTP is a life-threatening condition in which clots form in small blood vessels, resulting in a low overall platelet count. (
  • HIT is a life-threatening reaction to the drug heparin, given to patients to prevent the formation of blood clots. (
  • Platelets (green), the smallest blood cells, clump together into clots to stanch bleeding after an injury. (
  • Platelets are known for their role in forming blood clots, but more and more research shows that platelets help control our immune system as well. (
  • and platelets, also called thrombocytes, which assist in the formation of blood clots. (
  • Changes in blood platelets triggered by COVID-19 could contribute to the onset of heart attacks, strokes, and other serious complications in some patients who have the disease, according to University of Utah Health scientists. (
  • Emerging evidence suggests COVID-19 is associated with an increased risk of blood clotting, which can lead to cardiovascular problems and organ failure in some patients, particularly among those with underlying medical problems such as diabetes, obesity, or high blood pressure. (
  • They compared blood from these patients with samples taken from healthy individuals who were matched for age and sex. (
  • In test tube studies, the researchers found that pre-treating platelets from SARS-CoV-2 infected patients with aspirin did prevent this hyperactivity. (
  • 2020) Platelet Gene Expression and Function in COVID-19 Patients. (
  • The Red Cross must collect 15,000 blood donations every day to meet the needs of patients. (
  • All blood types are needed to ensure a reliable supply for patients. (
  • Her parents are also supportive of the Red Cross and host Red Cross blood drives in their daughter's honor to help meet the needs of other patients. (
  • Community Blood Centers of Florida collects more than 250,000 pints of blood and blood products for hospital and kidney dialysis patients, according to its website. (
  • Vast majority of dengue fever patients recover on their own with due care towards been kept properly hydrated, however, those, developing complications as severe decline in blood platelet are needed to be kept in strict vigilance of doctors and administered with needed blood units, elaborated the program manger of Sindh. (
  • Mayo Clinic recommends patients consult a medical professional to discuss complete blood count results that fall outside the normal ranges. (
  • Patients undergoing bone marrow transplants, surgeries, chemotherapy, radiation or organ transplants often need platelets to survive. (
  • Platelets have a very limited shelf life of five days and are delivered quickly to patients that need them. (
  • Patients at Dana-Farber and Brigham and Women's Hospital remain in need of blood products during the COVID-19 outbreak. (
  • When you donate blood or platelets at the Kraft Family Blood Donor Center at Dana-Farber Cancer Institute and Brigham and Women's Hospital, or give blood on board the Dana-Farber Cancer Institute/Brigham and Women's Hospital Blood Mobile, you are making a life-saving difference for patients in need - right here in our community. (
  • All of the blood and platelets collected at the Kraft Center and on the Blood Mobile benefit patients at Dana-Farber and Brigham and Women's Hospital. (
  • And platelets are really special, because that's what we're using for trauma victims, someone who has just got of surgery or cancer patients," said Red Cross Communications Manager Tammy Nakamura. (
  • Our results suggest that platelet activation, epinephrine, and high blood pressure play a role in the high prevalence of cerebrovascular and cardiovascular events in patients with OSAS. (
  • Platelet function analysis utilizing platelet-rich plasma and optical density based aggregometry fails to identify patients at risk for uremia associated complications. (
  • Ten chronic kidney disease (CKD) stage 4 or 5 predialysis patients underwent platelet evaluation. (
  • Diminished ATP release to arachidonic acid (an aspirin-like defect) in uremic patients may result in platelet associated bleeding. (
  • This platelet hyperreactivity may be associated with a thrombotic diathesis as seen in some uremic patients. (
  • Patients at City of Hope rely on more than 37,000 units of blood and platelets each year for their survival. (
  • To treat those patients, more than 41,000 blood donations are needed every day. (
  • Platelets, the clotting components found in blood, are crucial to cancer patients being treated with a bone marrow transplant. (
  • Each week, the Edward J. Miller, Sr. Blood Donor Center at Children's National needs 100 whole blood donations and 35 platelet donations to provide our pediatric patients with life-saving surgeries and to nurse children back to health from cancer or sickle cell disease. (
  • The Red Cross urges those who are eligible to help give blood or platelets to ensure patients around the country have access to lifesaving blood. (
  • Using the intracellularly trapped fluorescent dye quin2 , the free calcium concentration in platelets was found to be elevated in patients with borderline (n = 8, p less than 0.01) and established essential hypertension (n = 23, p less than 0.001) when compared with normotensive subjects (n = 30). (
  • after meeting all inclusion and exclusion criteria during the screening visit, those patients on aspirin for primary prevention of CV events will be asked to stop it for 2 weeks prior to blood collection for baseline data. (
  • Platelets benefiting patients at both Dana-Farber and Brigham and Women's are collected at the Kraft Family Blood Donor Center , located on the first floor of the Jimmy Fund Building at 35 Binney Street in Boston. (
  • Each day donations come up short, less blood is available for patients in need. (
  • Measure thrombocytopathy in a cohort of 70 Gaucher patients using a set of platelet function tests. (
  • Chisholm KM, Denton C, Keel S, Geddis AE, Xu M, Appel BE, Cantor AB, Fleming MD, Shimamura A. Bone Marrow Morphology Associated With Germline RUNX1 Mutations in Patients With Familial Platelet Disorder With Associated Myeloid Malignancy. (
  • Morrell, an associate professor at the University's Aab Cardiovascular Research Institute , says the idea that platelets keep immune cells in check is a new concept and his team is considering what it means for patients. (
  • Platelets are used in the treatment of cancer and leukemic patients, bone marrow transplant, new born babies and burns victims. (
  • Platelets are needed every day for the treatment of sick patients. (
  • So one of the ways we can keep our patients alive during the worst of the chemotherapy is we can replace those platelets with normal healthy donor blood. (
  • Around 28% of all blood donations are used by cancer patients. (
  • Patients receiving chemotherapy often develop low levels of red blood cells, a condition called chemotherapy-induced anemia. (
  • Patients with this condition will receive donor red blood cells that have been separated from the blood. (
  • For patients who have bleeding problems, studies may show a low platelet count. (
  • If you are safely able to leave your house, please consider giving blood for hospital patients," tweeted the Red Cross of Massachusetts . (
  • Platelet adhesiveness decreased markedly in six patients within ten minutes after intravenous injection. (
  • Platelets have another name, thrombocytes. (
  • Premature babies with low levels of platelets (thrombocytes) in their blood run a greatly increased risk of being afflicted with a severe variation of retinopathy of prematurity (ROP), an eye disease that can cause blindness, according to a study from Sweden and US published in the journal JCI Insight . (
  • Platelets (thrombocytes) are blood cell fragments and are essential for blood clotting. (
  • Platelets are well known for their role in blood clotting and blood vessel repair. (
  • If a blood vessel breaks, platelets cling to each other, forming a cork to plug the leak. (
  • When a blood vessel is damaged, platelets stick to the edges of it, clustering together to plug the hole. (
  • If the injury to the vessel is large, platelets alone will not stop the bleeding, so they release other factors that start blood clotting. (
  • Your blood pressure is the force of blood pushing against your blood vessel walls. (
  • When they find a damaged blood vessel, natural platelets bind to each other and signal other platelets to congregate at that spot. (
  • Platelets stop bleeding by clumping and forming plugs in blood vessel injuries. (
  • In this study, scientists from the Antibodies in Therapy and Pathology Unit (Institut Pasteur/Inserm U1222) revealed that platelets, whose best-known function is to stop bleeding when a blood vessel becomes damaged, play a key role in IgG-dependent anaphylactic reactions. (
  • The platelets attach to the damaged part of the blood vessel and become activated. (
  • These changes occur within seconds of blood vessel damage and expedites the rest of the clotting process. (
  • Although platelets are the first to respond to blood vessel injury, it cannot repair the breaks permanently. (
  • Community Blood Centers of Florida locations were inspected from September to December 2011, during which time staff found dangerous "deviations" from health protocol , according to letter sent in April and first reported by the South Florida Sun-Sentinel . (
  • The non-profit operates 15 donor centers in Palm Beach, Broward, Miami-Dade and Monroe Counties, and 50 blood mobiles also travel throughout South Florida and Central Florida. (
  • Bruce Blunt Jr. is alive today because of Ron Howard, the most prolific donor in the history of Florida's Blood Centers and possibly one of the top in the nation. (
  • The UCLA Blood & Platelet Center has two donor centers along with several community blood drives in your neighborhood. (
  • Platelets expressing IgG receptor FcγRIIA/CD32A determine the severity of experimental anaphylaxis, Science Immunology , April 13, 2018. (
  • I came on board the Gulf Coast Regional Blood Center in February 2018. (
  • 1) INTERFERON treatment causes a RAPID AND SUSTAINED reduction in peripheral platelet count by causing bone marrow suppression of platelets. (
  • Polycythemia is the blood illness where bone marrow makes too many red and white blood cells and too many platelets. (
  • These include failure to produce new platelets in the bone marrow, premature destruction of circulating platelets often by the body's own immune system, sequestration or storing of platelets in organs, and consumption of platelets at a rate that exceeds production in the bone marrow. (
  • Bone marrow aspiration to obtain a sample for laboratory analysis if there is concern that your dog's bone marrow may not be making adequate numbers of platelets or may have been invaded by cancer. (
  • They are made in the bone marrow and released to the blood, and they stick together to form clotting. (
  • Blood platelets are formed in bone marrow when a parent cell, a megakaryocyte, sends out arm-like extensions called proplatelets. (
  • has to go thro bone marrow transplantation and he needs A+ blood platelets on urgent basis. (
  • Stem cells are unique cells located in bone marrow or peripheral blood that can develop into red blood cells, white blood cells, and platelets. (
  • That's because individuals undergoing chemotherapy and radiation therapy typically lose the ability to make platelets in their bone marrow. (
  • Unlike other blood components such as red cells, which are refrigerated, platelets must be stored at room temperature to remain effective, but. (
  • The innate immune response is particularly important in fighting malaria, which causes symptoms once the parasite has invaded the victim s red blood cells. (
  • So they conducted similar experiments in a Petri dish in which they added human platelets to red blood cells infected with Plasmodium falciparum, the most deadly human malaria parasite. (
  • We believe that s because platelets bind to infected red blood cells and are taken out of circulation because of that, Foote said. (
  • Blood platelets are deceptively simple cells. (
  • However, as late as 1957 some workers still believed that platelets were products of other cells, such as the erythrocyte (Leiter, 1976). (
  • Surprisingly, Campbell and his colleagues didn't detect evidence of the virus in the vast majority of platelets, suggesting that it could be promoting the genetic changes within these cells indirectly. (
  • Uncovering the fundamental mechanisms of how platelets respond as aggregates vs individual cells provides the foundation for understanding the many roles of platelets beyond hemostasis. (
  • Platelets are about a quarter of the size of red blood cells and are not whole cells but rather fragile cell fragments. (
  • Platelets are blood cells that help control bleeding. (
  • This yellow liquid in the blood carries the RBCs, platelets, and cells or proteins, like antibodies, that help fight infections. (
  • 4. Histological examination of the lungs with a technique adequate to give a differential staining of platelet material demonstrates the presence of giant cells in the lungs, and supports the view that they are active in the production of platelets. (
  • 5. In extrauterine life giant cells are concentrated in the marrow and the lungs, with the maximum of their activity in platelet production in the lungs. (
  • The platelet also lives for a much shorter period of time, about a week compared with 4 months for red cells, so they have to be processed much more quickly. (
  • SALT LAKE CITY -- In a discovery that upends a longstanding tenet of human biology, University of Utah School of Medicine researchers have shown that a key process in gene regulation can occur in human platelets, unique cells that are unusual because they don't have a nucleus (anucleate). (
  • But using stem cells from human umbilical cord blood to engineer the precursor cell that forms platelets and platelets isolated from the blood of study subjects, the Utah researchers found that splicing also takes place in the cytoplasm of circulating platelets. (
  • Finding that platelets can splice the IL-1â pre-mRNA was completely unexpected and emerged while the researchers were engaged in earlier studies of how platelets communicate with certain leukocytes (white blood cells). (
  • Platelets are abundant cells that circulate in human blood and have many functions. (
  • It prevents white blood cells, which defend the body against foreign substances, from detecting them. (
  • Without the coating, white blood cells would destroy them in less than half an hour, he said. (
  • Other components of the blood transport metabolic waste from the cells to the kidneys, nutrients from the digestive system to the cells, and hormones throughout the body. (
  • If we do not have enough iron, our body makes fewer and smaller red blood cells, which means less hemoglobin, and therefore we do not get enough oxygen (WebMD, Iron Deficiency Anemia). (
  • These my include a complete blood count to look at your red blood cells and an iron test that shows how much iron is in your blood. (
  • This microscopic look at human blood reveals that nearly half of our blood is composed of red blood cells. (
  • When your platelets are taken, additional red cells and/or plasma may also be collected. (
  • T he U.S. Defense Department has awarded $3.5 million to a Cambridge, Mass., biotech that says it has figured out a way to make blood platelets from stem cells, a technology the military believes could be useful in treating battlefield and civilian casualties. (
  • 1) You draw the athlete's blood, centrifuge it (i.e. rotate it) at high speed, separating the red blood cells (RBC) from the plasma. (
  • 2) Your body will produce its own red blood cells to compensate from your blood loss. (
  • 3) You store the packed red blood cells in a refrigerator or freezer (RBC have a 120 day lifecycle). (
  • 4) Then just before competition, you re-inject the red blood cells back into your body. (
  • In CLL, lymphocytes (small white blood cells found mostly in the lymph system) become cancerous. (
  • We have examined the interactions of hemoglobin containing liposomes and of liposomes composed of polymerizable phospholipids with blood cells and proteins. (
  • In a study published today in The Journal of Clinical Investigation , Craig Morrell, DVM, Ph.D., discovered another important function of platelets: Keeping immune cells in balance. (
  • These cells are about 10 times or more large than red blood cells. (
  • Platelets have a lifespan of about 5 to 9 days in the circulation and old platelets are removed from the bloodstream by phagocytes in the spleen and Kupffer cells in the liver. (
  • There is also minimal risk of iron depletion because red blood cells are returned to the donor. (
  • If the patient has signs of anemia and studies show a low red blood cell (RBC) count, then red blood cells will be transfused. (
  • These harvested red blood cells are called "packed red blood cells" or PRBCs. (
  • I wonder why he has not giving you the Procrit shot that will boost those red blood cells fast. (
  • Red blood cells that carry oxygen can be refrigerated and used for up to 42 days. (
  • These cells extend long arms into nearby blood vessels. (
  • Donated platelets, when separated from the other blood components, are a mix of young and old cells. (
  • My group is developing a bioreactor that can make platelets from human stem cells. (
  • Cedric Ghevaert and colleagues at the University of Cambridge are among academic groups developing a different method of making platelets from stem cells that holds substantial promise . (
  • While my group is exposing stem cells to "outside-in" signals that help direct their differentiation toward platelets, Ghevaert's team is boosting the expression of key genes in the stem cells to effect "inside-out" signaling to drive the same differentiation process. (
  • Some versions of platelets have had the white blood cells partially removed or been gamma irradiated which have specific benefits for certain populations. (
  • For example, if a liver transplant patient has a low platelet count physicians may increase platelet levels before the transplant in order to limit rejection. (
  • Fox, J. E. B., 19856, Linkage of a membrane skeleton to integral membrane glycoproteins in human platelets. (
  • Similarly, if you bruise easily and always have, it may be due to another harmless condition of your body's blood. (
  • Pawlowski has spent much of her career studying platelets, part of the body's rapid defense force that swings into action when the body suffers anything from a paper cut to a severed leg. (
  • The blood serves as the body's major transport system. (
  • Platelets are the blood component serving as the body's "bandages. (
  • The platelets that release proteins and other particles are used in the body's self-healing process. (
  • In the last five to 10 years, scientists have found that platelets either initiate or accelerate the body's immune response in a wide range of inflammatory diseases, including atherosclerosis (when plaque builds up inside the arteries), arthritis and transplant rejection. (
  • More than 550 blood drives have been forced to cancel due to winter weather in January, causing over 16,500 blood and platelet donations to go uncollected through last week. (
  • The shortfall follows more than 26,400 uncollected blood and platelet donations in February because of severe weather across 27 states.In the River Valley Blood Blood Services Region, approximately 81 blood drives were canceled, causing more than 2,300 blood and nearly 50 platelet donations to go uncollected. (
  • If your low platelet count is caused by the treatment for hepatitis C or by chemotherapy, a doctor will likely reduce the amount you are receiving and monitor platelets carefully. (
  • Many of them will need blood - sometimes daily - during their chemotherapy treatment. (
  • If you are interested in becoming a platelet donor please take our Platelet Eligibility Quiz or call 01 4322833 (Dublin) or 021 4807429 (Cork) to speak to one of our staff. (
  • Platelets in the bloodstream contain factors that are like nannies for vascular development. (
  • But babies who are born prematurely consume much of their platelets in connection with infections, and an imbalance of these factors arises in the bloodstream and out in the tissue that can lead to pathological vascular development, in this case in the retina,' Ann Hellstrom explains. (
  • Platelets bud off from these extensions, separating from the cell body and nucleus, and mature platelets then enter the bloodstream. (
  • That's the question that Case Western Reserve University scientists answered by developing SynthoPlate, very tiny nanoparticles that, when injected into the bloodstream, mimic how platelets cluster to stop uncontrolled bleeding from various kinds of major trauma. (
  • Artificial platelets remain in the bloodstream for two to three days. (
  • Unlike blood doping, where it is injected into the bloodstream, the platelet-rich plasma is injected directly to the surrounding injury area and not into the bloodstream. (
  • Extra platelets are stored in the spleen while the remaining platelets circulate in the bloodstream. (
  • This blood test shows if you have antibodies against platelets in your blood. (
  • In the case of platelet antibodies, your body created antibodies to attack platelets. (
  • This means that you do not have anti-platelet antibodies in your blood. (
  • Abnormal results show that you have anti-platelet antibodies. (
  • The blood is also checked for any unexpected red blood cell antibodies that may cause problems for a recipient, as well as for diseases that could spread to recipients. (
  • The experimental parts of the study, conducted at Harvard Medical School in Boston, indicate that the pathological vascular development in the retina of young mice increased by 30 percent when platelet levels were lowered by means of antibodies. (
  • While it was already known that IgE antibodies can trigger these allergic reactions, scientists from the Institut Pasteur, Inserm, the CNRS and EFS Grand Est recently demonstrated that IgG antibodies play an active role in the severity of anaphylactic shock by unexpectedly activating blood platelets. (
  • To study the role of platelets in anaphylaxis, the scientists used transgenic mice expressing human receptors for IgG antibodies, since mice do not have these receptors on their platelets. (
  • Injecting these mice with human IgG antibodies triggered an anaphylactic reaction, characterized by a drop in body temperature and leading to a significant reduction in the circulating platelet count that lasted for several hours after the shock, suggesting that platelets might play a role in the allergic reaction. (
  • Due to low white blood count, I am careful about exposing self to anyone sick. (
  • It is too low for any treatment, but the doctor said in the next couple of months there will be a new trial coming out to increase the platelet count, but there are no guarantees. (
  • The addition of RIBAVIRIN actually HELPS sustain the platelet count. (
  • Dear Dr. Donohue: I had blood work done six months ago, and my platelet count was 470,000. (
  • When a patient has a high platelet count and no symptoms, treatment can be withheld. (
  • When symptoms do appear, then hydroxyurea or anagrelide can usually restore a normal platelet count. (
  • An organic diet rich in nutrients can have a beneficial effect on someone's platelet count. (
  • In a scientific study of mice with a blood platelet disorder, those who consumed 32 percent fewer calories had an improved platelet count and a longer lifespan. (
  • The National Heart, Lung, and Blood Institute shows normal ranges for the most common values detected on a blood test, including red blood cell count, whit. (
  • In healthy adults, the normal blood platelet count is 150,000 to 400,000 platelets per microliter of blood, according to MedlinePlus. (
  • In general, the lower the platelet count, the more likely bleeding is to occur. (
  • The main symptom of low blood platelet count is bleeding, which can present itself in many ways. (
  • Both purpura and petechiae are visible signs of a low blood platelet count. (
  • People also may suffer from low platelet count due to a chronic liver condition. (
  • If your low blood platelet count is due to ITP, you will likely be treated with immunosuppressants, which hinder the immune system from destroying platelets. (
  • The study, now published online in The British Journal of General Practice, revealed that high blood platelet count in some cases may be the only outward indication of cancer. (
  • TEHRAN (FNA)- Having a high blood platelet count is a strong predictor of cancer and should be urgently investigated to save lives, according to a large-scale study. (
  • Initial blood tests performed in the West Bank revealed a low blood platelet count. (
  • A normal platelet count is between 150 and 450 billion per liter for both men and women, according to Mayo Clinic. (
  • A platelet count that is lower or higher than normal is often a sign of an underlying medical condition, though it may also be a side effect of medication. (
  • A platelet count test is part of a complete blood count test, which also measures red blood cell count, hemoglobin, hematocrit and white blood cell count. (
  • When a platelet count is found to be outside the normal range, additional diagnostic testing is required to determine the cause. (
  • you should for sure ask about itp i also had it when i was younger and low platelet count is the biggest sign look out for any bruising or small red spots if he gets these you need to see your doctor asap. (
  • Conversely, increasing the platelet count in the blood resulted in a much worse allergic reaction. (
  • High Blood Platelet count! (
  • Is DH's platelet count to high? (
  • Thrombocytosis is a condition where the platelet count in the blood is higher than normal. (
  • This is contradictory to what would be expected with a high platelet count. (
  • It is more likely to arise with an excessively high platelet count and an associated deficiency of another component of blood clotting known as von Willebrand factor (vWF). (
  • Blood groups: A or O ( Rh positive or negative)Your availability, the suitability of your veins and platelet count are also factors we need to consider before accepting you as a platelet donor. (
  • There is now evidence that using a high platelet count threshold (50 x 109/L) increases the risk of death or bleeding compared to a lower platelet count threshold (25 x 109/L) in premature neonates. (
  • Guidelines recommend that it is safe to perform central venous catheter insertion when the platelet count is 20 x 109/L or above. (
  • The actual technical name for the platelet process is 'apheresis,'" said Nokamura. (
  • An apheresis machine separates anticoagulated blood into components with retention of the platelets and a portion of plasma to create a standard adult dose of apheresis plateletes. (
  • When requested, an apheresis platelet unit can be further divided into four packs of equal volume to produce paediatric apheresis platelet components. (
  • Both apheresis and pooled platelets are leucodepleted during or soon after collection and they are also irradiated before release from the Blood Service, unless other specific arrangements have been made with the receiving laboratory/institution. (
  • Apheresis platelets can be modified as phenotyped , CMV-seronegative and HLA-compatible , crossmatch-compatible and/or low anti-A/B . (
  • Platelets can be produced either from whole blood or by apheresis. (
  • Make an appointment to give blood or platelets by downloading the free Red Cross Blood Donor App, visiting or calling 1-800-RED CROSS (1-800-733-2767). (
  • Please contact the Kraft Family Blood Donor Center at 617.632.3206 or email us at [email protected] . (
  • At the Kraft Family Blood Donor Center, located on the first floor of the Jimmy Fund Building at 35 Binney Street in Boston. (
  • Please contact blood donor center with questions or if work on a specific procedure is ongoing. (
  • Medications or medical conditions: Please contact the blood donor center. (
  • Travel outside USA: If within 12 months, please contact the blood donor center with detailed dates and locations. (
  • It contains a higher concentration of blood-clotting proteins than regular plasma. (
  • During that investigation they found evidence of platelets making new proteins, which led them to pursue the mechanisms that are involved, said Guy A. Zimmerman, M.D., professor of internal medicine and one of the study's co-authors. (
  • The idea that blood platelets could make proteins without having a nucleus had been thought heretical," said Zimmerman, who also heads the U's Program in Human Molecular Biology and Genetics at the Eccles Institute of Human Genetics. (
  • Healing injuries involves a well orchestrated and complex series of events where proteins in the blood called growth factors act as messengers, regulating the entire process.There is a growing interest for Autologous blood products for use in a number of orthopaedic procedures. (
  • Interactions of Lipid Vesicles with Blood Proteins and Platelets. (
  • In a healthy person, a large number of platelets are manufactured and stored in the body. (
  • Individuals who are 17 years of age (16 with parental consent in some states), weigh at least 110 pounds and are in generally good health may be eligible to donate blood. (
  • BE A HERO THIS VALENTINE'S DAY Schedule an appointment to donate blood or platelets using the Red Cross Blood Donor App , visiting , or calling 1-800-RED CROSS (1-800-733-2767). (
  • Individuals who are 17 years of age (16 with parental consent in most states), weigh at least 110 pounds and are in generally good health may be eligible to donate blood or platelets. (
  • If you'd like to donate blood or platelets, a good first step is to find your local Red Cross blood drive or blood bank where you can donate. (
  • Certain medications may delay your ability to donate blood. (
  • DO NOT discontinue medications prescribed or recommended by your physicians in order to donate blood. (
  • Hepatitis B Immune Globulin (HBIG) is an injected material used to prevent hepatitis B infection following a possible or known exposure to hepatitis B. HBIG does not prevent hepatitis B infection in every case, therefore, persons who have received HBIG must wait to donate blood. (
  • So if you're looking to give back this holiday season, consider taking an hour or so to donate blood and help save a life. (
  • Am I Eligible to Donate Blood? (
  • Find out about the eligibility requirements to donate blood today. (
  • Helping Hurricane Harvey victims: Donate blood, platelets & plasma! (
  • If you'd like to donate blood, you can find locations and times for Life South here and for the American Red Cross here . (
  • You may donate blood if it has been more than 12 months since incarceration or sexual contact with someone who was incarcerated. (
  • You may not donate blood if you have used illegal intravenous drugs or if you take anabolic steroids intravenously (unless you have a doctor's prescription) in the past 3 months . (
  • It is medically safe to donate blood about every 60 days, plasma once a month and platelets every one or two weeks. (
  • Anyone who can donate blood or platelets please turn out. (
  • To make an appointment to donate blood or platelets, please call 212-639-8177 or 212-639-7648. (
  • One way of addressing this problem would be to get more people to donate blood, although I'm not sure how realistic that is. (
  • About 7 million people already donate blood in the U.S. each year. (
  • I regularly donate blood, I did on my last cycle(androhard/tbol) like nos said if they see something they don't like they'll toss it, there main concentrate is HIV, and hepatitis. (
  • To donate blood, individuals must meet minimum age requirements (16-year-olds in Oregon need parental consent, 16-and 17-year-olds in Washington need parental consent), weigh at least 110 pounds and be generally healthy. (
  • Artificial platelets have a longer shelf life, allowing first-responders and more hospitals could keep them on hand. (
  • Platelets can be irradiated at any stage during their 5 day storage and thereafter can be stored up to their normal shelf life of 5 days after collection. (
  • With a shelf life of just five days, platelet donations are especially needed. (
  • Platelets have a shelf life of 5 days. (
  • But platelets - the Band-Aids of the body that stop bleeding - have a shelf life of only two days after they are screened for disease. (
  • Given their short shelf life, platelets must be screened for bacteria and viruses quickly, and immediately delivered to individuals who need them. (
  • Whole blood platelet analysis detected platelet dysfunction which may be associated with bleeding and thrombotic risks in uremia. (
  • New studies in mice suggest that blood platelets can destroy deadly malaria parasites, but a single dose of aspirin may be enough to thwart their killing power. (
  • To ensure that their original observation was not due to other genetic changes caused by knocking out the Mpl gene, the researchers eliminated platelets by giving the mice aspirin, which inactivates platelets. (
  • Although there is more work to do, the researchers believe that the aspirin is preventing some sort of anti-malarial effect produced by the platelets. (
  • And when aspirin was added, the platelets no longer held back the parasites. (
  • Platelet samples came from another 2,000 similar participants, including 800 blacks, enrolled in the Genetic Study of Aspirin Responsiveness (GeneSTAR) under way at Johns Hopkins since 2002 and led by Becker's wife and study co-investigator Diane Becker, M.P.H., Sc.D., a professor at the both Hopkins' School of Medicine and the University's Bloomberg School of Public Health. (
  • Lewis Becker says the teams' next steps are to test various platelet antagonists, or blood-thinning agents, like aspirin, the most common drug treatment in heart and vascular diseases, to find out precisely which hereditary factors may distinguish people who are so-called aspirin-resistant or not, and why the medication works for most but not all. (
  • Please be sure to not consume aspirin in the 48 hours prior to donating platelets. (
  • Hepatitis C is a blood-borne viral disease which can cause liver inflammation, fibrosis, cirrhosis and liver cancer. (
  • They also noted that these changes significantly altered how platelets interacted with the immune system, likely contributing to inflammation of the respiratory tract that may, in turn, result in more severe lung injury. (
  • 2 More recently, there is a growing appreciation for the critical role platelets have in maintaining vascular integrity during development and inflammation. (
  • Our main goal is to dissect the molecular and mechanical cues regulating platelet cytoskeleton dynamics and platelet motility in the context of thrombosis and inflammation. (
  • The U researchers, who report their findings in the Aug. 12 edition of Cell, also identified the pre-mRNA in blood platelets that codes for Interleukin 1â (IL-1â), a key protein in an ancient molecular system that plays major roles in inflammation, defense against infection, organ development, and disease. (
  • Information is based on the typical unit content (mean ± 1 SD) from Blood Service data (1 January to 31 December 2017 inclusive) and acceptable specification limits (in parentheses). (
  • Previous studies have shown that platelets are active in the body s innate immune system, which responds rapidly to invading pathogens. (
  • Corticosteroids (cortisone-like drugs) are often used to stop the immune system from destroying platelets. (
  • as a suggestion, at printout all the research about immune system and blood trasfusions. (
  • The hepatitis C virus (HCV) is spread by blood-to-blood contact with an infected person's blood. (
  • elimination of these foods from a person's diet can promote platelet buildup. (
  • In most instances this high levels of platelets does not pose any significant threat to the person's health. (
  • In another test, called a platelet function test, a sample of blood is collected and tested to see if platelets react normally to various platelet stimulators. (
  • Fresh frozen plasma and platelets, if transfused unnecessarily, it can lead to reactions. (
  • After they reach their local quota, they'll send extra pints of blood, plasma and platelets down to the Greater Houston area. (
  • But there are never enough platelets to help everyone who needs them, largely because of their ephemeral nature. (
  • Approximately 750 billion platelets circulate in human blood, constantly scanning the vasculature for damage of the endothelial surface. (
  • Platelet BioGenesis, which was founded in 2014, said Wednesday that the grant will help the startup develop a bioreactor that is key to the production of platelets, a critical component of blood that promotes clotting. (
  • The current study points to a correlation between low levels of platelets, whose main task in adults is to inhibit bleeding in blood vessels, and a fourfold increased risk of severe ROP in infants. (
  • This leads to high levels of platelets in the circulation. (
  • The biggest risk in the nation's blood supply is no longer HIV or hepatitis C, it's bacterial contamination of platelets, resulting in at least 20 deaths and hundreds of adverse reactions in recent years, health experts say. (
  • In experiments on mice, injections of blood platelets reduce the pathological development of retinal vessels. (
  • Their primary role is to form "plugs" that stop bleeding from injured blood vessels. (
  • When the inner lining of blood vessels (endothelium) is damaged, platelets are one of the first blood components to respond. (
  • Low-risk procedures include surgical sites that do not contain many blood vessels e.g. cataract surgery, or minor procedures. (
  • We recently developed imaging based tools to visualize these processes on a single platelet level in vitro and in vivo . (
  • The condition in which there are too many platelets is thrombocytosis. (
  • That means you have essential thrombocytosis, an increased number of platelets without known cause. (
  • Having too many platelets in the blood is a sign of either thrombocytosis or thrombocythemia. (
  • In thrombocytosis, the number of platelets produced is higher than normal and these platelets have a longer lifespan. (
  • Low platelets can be the result of an autoimmune problem, so I was wondering if petechiae could be another disorder from celiac. (
  • According to the Platelet Disorder Support Association, a change in diet can help build up blood platelets. (
  • The Platelet Disorder Support Association suggests eating fruits and vegetables and eliminating harmful foods that contain few nutrients, vitamins or minerals. (
  • While most high school seniors were preparing to go off to college, Briana was fighting for her life against a rare blood disorder, called severe aplastic anemia. (
  • Also searched for Platelet disorder . (
  • Chronic lymphocytic leukemia (CLL) is a generally slow-growing blood disorder. (
  • Von Willebrand disease is the most common inherited platelet-related disorder. (
  • Abnormal platelet function tests may indicate the nature of the inherited or acquired platelet dysfunction. (
  • Because the body manufactures and stores extra platelets, the platelets collected during the procedure will be replaced almost immediately. (
  • During those studies, they discovered that platelet-deficient mice were much more likely to die of malaria than mice with normal platelets. (
  • Platelet dysfunction may be due to a problem in the platelets themselves or to an external factor that alters the function of normal platelets. (
  • The reason bleeding occurs lies in the fact that the numerous platelets don't function normally. (
  • At present, blood banks function like business units, they supply blood and its components according to the demand. (
  • In what is believed to be the largest review of the human genetic code to determine why some people's blood platelets are more likely to clump faster than others, scientists at Johns Hopkins and in Boston have found a septet of overactive genes, which they say likely control that bodily function. (
  • Statin drugs alter platelet function. (
  • As a biomedical engineer, she was fascinated with how platelets function, and the idea that scientists could build something to mimic them. (
  • To confirm their function, the scientists eliminated the platelets from the blood, which led to a reduction in symptoms. (
  • When platelets do not function properly, people are at risk of excessive bleeding due to injuries or even spontaneous bleeding. (
  • Its function is to carry oxygen-rich blood from the heart to the rest of the body. (
  • Average healthy adults produce some 100 billion platelets each day. (
  • Dogs with blood platelet concentrations of less than 40,000 per microliter of blood are at risk for spontaneous bleeding. (
  • Bacterial contamination of platelet components occurs because the storage temperature for platelets (22° C) may facilitate bacterial growth. (
  • However, a similar reaction occurs in people who are given heparin, which triggers extreme blood clotting that depletes their platelets. (
  • There is also an urgent need for platelet donations. (