Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation.
The attachment of PLATELETS to one another. This clumping together can be induced by a number of agents (e.g., THROMBIN; COLLAGEN) and is part of the mechanism leading to the formation of a THROMBUS.
The number of PLATELETS per unit volume in a sample of venous BLOOD.
The process whereby PLATELETS adhere to something other than platelets, e.g., COLLAGEN; BASEMENT MEMBRANE; MICROFIBRILS; or other "foreign" surfaces.
Surface glycoproteins on platelets which have a key role in hemostasis and thrombosis such as platelet adhesion and aggregation. Many of these are receptors.
A CXC chemokine that is found in the alpha granules of PLATELETS. The protein has a molecular size of 7800 kDa and can occur as a monomer, a dimer or a tetramer depending upon its concentration in solution. Platelet factor 4 has a high affinity for HEPARIN and is often found complexed with GLYCOPROTEINS such as PROTEIN C.
An enzyme formed from PROTHROMBIN that converts FIBRINOGEN to FIBRIN.
Adenosine 5'-(trihydrogen diphosphate). An adenine nucleotide containing two phosphate groups esterified to the sugar moiety at the 5'-position.
The transfer of blood platelets from a donor to a recipient or reinfusion to the donor.
A biochemical messenger and regulator, synthesized from the essential amino acid L-TRYPTOPHAN. In humans it is found primarily in the central nervous system, gastrointestinal tract, and blood platelets. Serotonin mediates several important physiological functions including neurotransmission, gastrointestinal motility, hemostasis, and cardiovascular integrity. Multiple receptor families (RECEPTORS, SEROTONIN) explain the broad physiological actions and distribution of this biochemical mediator.
Platelet membrane glycoprotein complex important for platelet adhesion and aggregation. It is an integrin complex containing INTEGRIN ALPHAIIB and INTEGRIN BETA3 which recognizes the arginine-glycine-aspartic acid (RGD) sequence present on several adhesive proteins. As such, it is a receptor for FIBRINOGEN; VON WILLEBRAND FACTOR; FIBRONECTIN; VITRONECTIN; and THROMBOSPONDINS. A deficiency of GPIIb-IIIa results in GLANZMANN THROMBASTHENIA.
Platelet membrane glycoprotein complex essential for normal platelet adhesion and clot formation at sites of vascular injury. It is composed of three polypeptides, GPIb alpha, GPIb beta, and GPIX. Glycoprotein Ib functions as a receptor for von Willebrand factor and for thrombin. Congenital deficiency of the GPIb-IX complex results in Bernard-Soulier syndrome. The platelet glycoprotein GPV associates with GPIb-IX and is also absent in Bernard-Soulier syndrome.
Very large BONE MARROW CELLS which release mature BLOOD PLATELETS.
A platelet-specific protein which is released when platelets aggregate. Elevated plasma levels have been reported after deep venous thrombosis, pre-eclampsia, myocardial infarction with mural thrombosis, and myeloproliferative disorders. Measurement of beta-thromboglobulin in biological fluids by radioimmunoassay is used for the diagnosis and assessment of progress of thromboembolic disorders.
A series of progressive, overlapping events, triggered by exposure of the PLATELETS to subendothelial tissue. These events include shape change, adhesiveness, aggregation, and release reactions. When carried through to completion, these events lead to the formation of a stable hemostatic plug.
Disorders caused by abnormalities in platelet count or function.
A subnormal level of BLOOD PLATELETS.
A phospholipid derivative formed by PLATELETS; BASOPHILS; NEUTROPHILS; MONOCYTES; and MACROPHAGES. It is a potent platelet aggregating agent and inducer of systemic anaphylactic symptoms, including HYPOTENSION; THROMBOCYTOPENIA; NEUTROPENIA; and BRONCHOCONSTRICTION.
Laboratory examination used to monitor and evaluate platelet function in a patient's blood.
Plasma glycoprotein clotted by thrombin, composed of a dimer of three non-identical pairs of polypeptide chains (alpha, beta, gamma) held together by disulfide bonds. Fibrinogen clotting is a sol-gel change involving complex molecular arrangements: whereas fibrinogen is cleaved by thrombin to form polypeptides A and B, the proteolytic action of other enzymes yields different fibrinogen degradation products.
2-Octylcyclopentaneheptanoic acids. The family of saturated carbon-20 cyclic fatty acids that represent the parent compounds of the prostaglandins.
Cell adhesion molecule and CD antigen that mediates the adhesion of neutrophils and monocytes to activated platelets and endothelial cells.
A high-molecular-weight plasma protein, produced by endothelial cells and megakaryocytes, that is part of the factor VIII/von Willebrand factor complex. The von Willebrand factor has receptors for collagen, platelets, and ristocetin activity as well as the immunologically distinct antigenic determinants. It functions in adhesion of platelets to collagen and hemostatic plug formation. The prolonged bleeding time in VON WILLEBRAND DISEASES is due to the deficiency of this factor.
The prototypical analgesic used in the treatment of mild to moderate pain. It has anti-inflammatory and antipyretic properties and acts as an inhibitor of cyclooxygenase which results in the inhibition of the biosynthesis of prostaglandins. Aspirin also inhibits platelet aggregation and is used in the prevention of arterial and venous thrombosis. (From Martindale, The Extra Pharmacopoeia, 30th ed, p5)
The process of generating thrombocytes (BLOOD PLATELETS) from the pluripotent HEMATOPOIETIC STEM CELLS in the BONE MARROW via the MEGAKARYOCYTES. The humoral factor with thrombopoiesis-stimulating activity is designated THROMBOPOIETIN.
A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).
The number of LEUKOCYTES and ERYTHROCYTES per unit volume in a sample of venous BLOOD. A complete blood count (CBC) also includes measurement of the HEMOGLOBIN; HEMATOCRIT; and ERYTHROCYTE INDICES.
An unstable intermediate between the prostaglandin endoperoxides and thromboxane B2. The compound has a bicyclic oxaneoxetane structure. It is a potent inducer of platelet aggregation and causes vasoconstriction. It is the principal component of rabbit aorta contracting substance (RCS).
The process of the interaction of BLOOD COAGULATION FACTORS that results in an insoluble FIBRIN clot.
The process which spontaneously arrests the flow of BLOOD from vessels carrying blood under pressure. It is accomplished by contraction of the vessels, adhesion and aggregation of formed blood elements (eg. ERYTHROCYTE AGGREGATION), and the process of BLOOD COAGULATION.
Human alloantigens expressed only on platelets, specifically on platelet membrane glycoproteins. These platelet-specific antigens are immunogenic and can result in pathological reactions to transfusion therapy.
Formation and development of a thrombus or blood clot in the blood vessel.
A humoral factor that stimulates the production of thrombocytes (BLOOD PLATELETS). Thrombopoietin stimulates the proliferation of bone marrow MEGAKARYOCYTES and their release of blood platelets. The process is called THROMBOPOIESIS.
Condensed areas of cellular material that may be bounded by a membrane.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
An opioid analgesic chemically related to and with an action resembling that of MEPERIDINE, but more rapid in onset and of shorter duration. It has been used in obstetrics, as pre-operative medication, for minor surgical procedures, and for dental procedures. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1067)
Physiologically active compounds found in many organs of the body. They are formed in vivo from the prostaglandin endoperoxides and cause platelet aggregation, contraction of arteries, and other biological effects. Thromboxanes are important mediators of the actions of polyunsaturated fatty acids transformed by cyclooxygenase.
A mixture of the mesylates (methane sulfonates) of DIHYDROERGOCORNINE; DIHYDROERGOCRISTINE; and the alpha- and beta-isomers of DIHYDROERGOCRYPTINE. The substance produces a generalized peripheral vasodilation and a fall in arterial pressure and has been used to treat symptoms of mild to moderate impairment of mental function in the elderly.
A phosphodiesterase inhibitor which inhibits platelet aggregation. Formerly used as an antineoplastic.
The process by which blood or its components are kept viable outside of the organism from which they are derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).
A plant genus of the family ROSACEAE. The common names of chokeberry or chokecherry are also used for some species of PRUNUS.
A deficiency or absence of FIBRINOGEN in the blood.
Duration of blood flow after skin puncture. This test is used as a measure of capillary and platelet function.
A vasoconstrictor found in ergot of Central Europe. It is a serotonin agonist that has been used as an oxytocic agent and in the treatment of MIGRAINE DISORDERS.
A tricyclic antidepressant that has actions and uses similar to those of AMITRIPTYLINE, but has only weak antimuscarinic and sedative effects. (From Martindale, The Extra Pharmacopoeia, 30th ed, p257)
Cell surface proteins that bind THROMBOXANES with high affinity and trigger intracellular changes influencing the behavior of cells. Some thromboxane receptors act via the inositol phosphate and diacylglycerol second messenger systems.
The rate dynamics in chemical or physical systems.
Proteins that are present in blood serum, including SERUM ALBUMIN; BLOOD COAGULATION FACTORS; and many other types of proteins.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
The active sympathomimetic hormone from the ADRENAL MEDULLA. It stimulates both the alpha- and beta- adrenergic systems, causes systemic VASOCONSTRICTION and gastrointestinal relaxation, stimulates the HEART, and dilates BRONCHI and cerebral vessels. It is used in ASTHMA and CARDIAC FAILURE and to delay absorption of local ANESTHETICS.
Platelet membrane glycoprotein IIb is an integrin alpha subunit that heterodimerizes with INTEGRIN BETA3 to form PLATELET GLYCOPROTEIN GPIIB-IIIA COMPLEX. It is synthesized as a single polypeptide chain which is then postranslationally cleaved and processed into two disulfide-linked subunits of approximately 18 and 110 kDa in size.
A prostaglandin that is a powerful vasodilator and inhibits platelet aggregation. It is biosynthesized enzymatically from PROSTAGLANDIN ENDOPEROXIDES in human vascular tissue. The sodium salt has been also used to treat primary pulmonary hypertension (HYPERTENSION, PULMONARY).
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
A phospholipid from the platelet membrane that contributes to the blood clotting cascade by forming a phospholipid-protein complex (THROMBOPLASTIN) which serves as a cofactor with FACTOR VIIA to activate FACTOR X in the extrinsic pathway of BLOOD COAGULATION.
The prototypical tricyclic antidepressant. It has been used in major depression, dysthymia, bipolar depression, attention-deficit disorders, agoraphobia, and panic disorders. It has less sedative effect than some other members of this therapeutic group.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
An organization of cells into an organ-like structure. Organoids can be generated in culture. They are also found in certain neoplasms.
Hemorrhagic and thrombotic disorders that occur as a consequence of abnormalities in blood coagulation due to a variety of factors such as COAGULATION PROTEIN DISORDERS; BLOOD PLATELET DISORDERS; BLOOD PROTEIN DISORDERS or nutritional conditions.
The network of filaments, tubules, and interconnecting filamentous bridges which give shape, structure, and organization to the cytoplasm.
An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.
Drugs or agents which antagonize or impair any mechanism leading to blood platelet aggregation, whether during the phases of activation and shape change or following the dense-granule release reaction and stimulation of the prostaglandin-thromboxane system.
Elements of limited time intervals, contributing to particular results or situations.
A group of compounds derived from unsaturated 20-carbon fatty acids, primarily arachidonic acid, via the cyclooxygenase pathway. They are extremely potent mediators of a diverse group of physiological processes.
An ionophorous, polyether antibiotic from Streptomyces chartreusensis. It binds and transports CALCIUM and other divalent cations across membranes and uncouples oxidative phosphorylation while inhibiting ATPase of rat liver mitochondria. The substance is used mostly as a biochemical tool to study the role of divalent cations in various biological systems.
An unsaturated, essential fatty acid. It is found in animal and human fat as well as in the liver, brain, and glandular organs, and is a constituent of animal phosphatides. It is formed by the synthesis from dietary linoleic acid and is a precursor in the biosynthesis of prostaglandins, thromboxanes, and leukotrienes.
Cell surface receptors that are specific for THROMBOPOIETIN. They signal through interaction with JANUS KINASES such as JANUS KINASE 2.
A chelating agent that sequesters a variety of polyvalent cations such as CALCIUM. It is used in pharmaceutical manufacturing and as a food additive.
An ergot derivative that is a congener of LYSERGIC ACID DIETHYLAMIDE. It antagonizes the effects of serotonin in blood vessels and gastrointestinal smooth muscle, but has few of the properties of other ergot alkaloids. Methysergide is used prophylactically in migraine and other vascular headaches and to antagonize serotonin in the carcinoid syndrome.
Field of chemistry that pertains to immunological phenomena and the study of chemical reactions related to antigen stimulation of tissues. It includes physicochemical interactions between antigens and antibodies.
The sum of the weight of all the atoms in a molecule.
A subclass of purinergic P2Y receptors that have a preference for ADP binding and are coupled to GTP-BINDING PROTEIN ALPHA SUBUNIT, GI. The P2Y12 purinergic receptors are found in PLATELETS where they play an important role regulating PLATELET ACTIVATION.
Cell surface receptors that bind prostaglandins with high affinity and trigger intracellular changes which influence the behavior of cells. Prostaglandin receptor subtypes have been tentatively named according to their relative affinities for the endogenous prostaglandins. They include those which prefer prostaglandin D2 (DP receptors), prostaglandin E2 (EP1, EP2, and EP3 receptors), prostaglandin F2-alpha (FP receptors), and prostacyclin (IP receptors).
Organic compounds that contain 1,2-diphenylethylene as a functional group.
Antibodies produced by a single clone of cells.
Disorder characterized by a decrease or lack of platelet dense bodies in which the releasable pool of adenine nucleotides and 5HT are normally stored.
A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts.
An antibiotic mixture of two components, A and B, obtained from Nocardia lurida (or the same substance produced by any other means). It is no longer used clinically because of its toxicity. It causes platelet agglutination and blood coagulation and is used to assay those functions in vitro.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
A congenital bleeding disorder with prolonged bleeding time, absence of aggregation of platelets in response to most agents, especially ADP, and impaired or absent clot retraction. Platelet membranes are deficient in or have a defect in the glycoprotein IIb-IIIa complex (PLATELET GLYCOPROTEIN GPIIB-IIIA COMPLEX).
The relationship between the dose of an administered drug and the response of the organism to the drug.
A protein factor that regulates the length of R-actin. It is chemically similar, but immunochemically distinguishable from actin.
Peptides composed of between two and twelve amino acids.
Any form of purpura in which the PLATELET COUNT is decreased. Many forms are thought to be caused by immunological mechanisms.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.
Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.
A dynamic actin-rich extension of the surface of an animal cell used for locomotion or prehension of food.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
Venoms from snakes of the subfamily Crotalinae or pit vipers, found mostly in the Americas. They include the rattlesnake, cottonmouth, fer-de-lance, bushmaster, and American copperhead. Their venoms contain nontoxic proteins, cardio-, hemo-, cyto-, and neurotoxins, and many enzymes, especially phospholipases A. Many of the toxins have been characterized.
An alkaloid found in the roots of Rauwolfia serpentina and R. vomitoria. Reserpine inhibits the uptake of norepinephrine into storage vesicles resulting in depletion of catecholamines and serotonin from central and peripheral axon terminals. It has been used as an antihypertensive and an antipsychotic as well as a research tool, but its adverse effects limit its clinical use.
A chelating agent relatively more specific for calcium and less toxic than EDETIC ACID.
The quantity of volume or surface area of CELLS.
A layer of epithelium that lines the heart, blood vessels (ENDOTHELIUM, VASCULAR), lymph vessels (ENDOTHELIUM, LYMPHATIC), and the serous cavities of the body.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Increased numbers of platelets in the peripheral blood. (Dorland, 27th ed)
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
Endogenous substances, usually proteins, that are involved in the blood coagulation process.
A preparation consisting of PLATELETS concentrated in a limited volume of PLASMA. This is used in various surgical tissue regeneration procedures where the GROWTH FACTORS in the platelets enhance wound healing and regeneration.
A stable, physiologically active compound formed in vivo from the prostaglandin endoperoxides. It is important in the platelet-release reaction (release of ADP and serotonin).
Adherence of cells to surfaces or to other cells.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Synthetic compounds that are analogs of the naturally occurring prostaglandin endoperoxides and that mimic their pharmacologic and physiologic activities. They are usually more stable than the naturally occurring compounds.
Organic nitrogenous bases. Many alkaloids of medical importance occur in the animal and vegetable kingdoms, and some have been synthesized. (Grant & Hackh's Chemical Dictionary, 5th ed)
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
The number of WHITE BLOOD CELLS per unit volume in venous BLOOD. A differential leukocyte count measures the relative numbers of the different types of white cells.
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.
Stable carbon atoms that have the same atomic number as the element carbon, but differ in atomic weight. C-13 is a stable carbon isotope.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.
The development and formation of various types of BLOOD CELLS. Hematopoiesis can take place in the BONE MARROW (medullary) or outside the bone marrow (HEMATOPOIESIS, EXTRAMEDULLARY).
A familial coagulation disorder characterized by a prolonged bleeding time, unusually large platelets, and impaired prothrombin consumption.
Proteins prepared by recombinant DNA technology.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
A highly polar organic liquid, that is used widely as a chemical solvent. Because of its ability to penetrate biological membranes, it is used as a vehicle for topical application of pharmaceuticals. It is also used to protect tissue during CRYOPRESERVATION. Dimethyl sulfoxide shows a range of pharmacological activity including analgesia and anti-inflammation.
A purely physical condition which exists within any material because of strain or deformation by external forces or by non-uniform thermal expansion; expressed quantitatively in units of force per unit area.
A family of proteinase-activated receptors that are specific for THROMBIN. They are found primarily on PLATELETS and on ENDOTHELIAL CELLS. Activation of thrombin receptors occurs through the proteolytic action of THROMBIN, which cleaves the N-terminal peptide from the receptor to reveal a new N-terminal peptide that is a cryptic ligand for the receptor. The receptors signal through HETEROTRIMERIC GTP-BINDING PROTEINS. Small synthetic peptides that contain the unmasked N-terminal peptide sequence can also activate the receptor in the absence of proteolytic activity.
The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)
A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Drugs that bind to but do not activate serotonin receptors, thereby blocking the actions of serotonin or SEROTONIN RECEPTOR AGONISTS.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
The preparation of platelet concentrates with the return of red cells and platelet-poor plasma to the donor.
A calcium-activated enzyme that catalyzes the hydrolysis of ATP to yield AMP and orthophosphate. It can also act on ADP and other nucleoside triphosphates and diphosphates. EC
Univalent antigen-binding fragments composed of one entire IMMUNOGLOBULIN LIGHT CHAIN and the amino terminal end of one of the IMMUNOGLOBULIN HEAVY CHAINS from the hinge region, linked to each other by disulfide bonds. Fab contains the IMMUNOGLOBULIN VARIABLE REGIONS, which are part of the antigen-binding site, and the first IMMUNOGLOBULIN CONSTANT REGIONS. This fragment can be obtained by digestion of immunoglobulins with the proteolytic enzyme PAPAIN.
A stable prostaglandin endoperoxide analog which serves as a thromboxane mimetic. Its actions include mimicking the hydro-osmotic effect of VASOPRESSIN and activation of TYPE C PHOSPHOLIPASES. (From J Pharmacol Exp Ther 1983;224(1): 108-117; Biochem J 1984;222(1):103-110)
A metallic element, atomic number 49, atomic weight 114.82, symbol In. It is named from its blue line in the spectrum. (From Dorland, 28th ed)
Slender, cylindrical filaments found in the cytoskeleton of plant and animal cells. They are composed of the protein TUBULIN and are influenced by TUBULIN MODULATORS.
Collagen receptors are cell surface receptors that modulate signal transduction between cells and the EXTRACELLULAR MATRIX. They are found in many cell types and are involved in the maintenance and regulation of cell shape and behavior, including PLATELET ACTIVATION and aggregation, through many different signaling pathways and differences in their affinities for collagen isoforms. Collagen receptors include discoidin domain receptors, INTEGRINS, and glycoprotein VI.
Proteins that bind specific drugs with high affinity and trigger intracellular changes influencing the behavior of cells. Drug receptors are generally thought to be receptors for some endogenous substance not otherwise specified.
An absence of warmth or heat or a temperature notably below an accustomed norm.
The deformation and flow behavior of BLOOD and its elements i.e., PLASMA; ERYTHROCYTES; WHITE BLOOD CELLS; and BLOOD PLATELETS.
The part of a cell that contains the CYTOSOL and small structures excluding the CELL NUCLEUS; MITOCHONDRIA; and large VACUOLES. (Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Thrombocytopenia occurring in the absence of toxic exposure or a disease associated with decreased platelets. It is mediated by immune mechanisms, in most cases IMMUNOGLOBULIN G autoantibodies which attach to platelets and subsequently undergo destruction by macrophages. The disease is seen in acute (affecting children) and chronic (adult) forms.
A family of related, adhesive glycoproteins which are synthesized, secreted, and incorporated into the extracellular matrix of a variety of cells, including alpha granules of platelets following thrombin activation and endothelial cells. They interact with a number of BLOOD COAGULATION FACTORS and anticoagulant factors. Five distinct forms have been identified, thrombospondin 1, -2, -3, -4, and cartilage oligomeric matrix protein (COMP). They are involved in cell adhesion, platelet aggregation, cell proliferation, angiogenesis, tumor metastasis, VASCULAR SMOOTH MUSCLE growth, and tissue repair.
A rare, inherited platelet disorder characterized by a selective deficiency in the number and contents of platelet alpha-granules. It is associated with THROMBOCYTOPENIA, enlarged platelets, and prolonged bleeding time.
Bleeding or escape of blood from a vessel.

Values of three coagulation screening tests of precolostral calves. (1/11030)

Prothrombin times, partial thromboplastin times and platelet counts were performed to determine normal values and to screen for coagulation defects of precolostral calves. The precolostral calves were in two groups: one group of a few calves was tested two years before the second larger group. The results for both groups were similar. The tests were performed on postcolostral calves and on mature cows to compare their values with those of precolostral calves. The mean values of prothrombin times and partial thromboplastin times of precolostral calves in the first group were 18.8 seconds and 54.8 seconds respectively. The mean values of prothrombin times and partial thromboplastin times of precolostral calves in the second group were 18.8 seconds and 50.8 seconds respectively. The mean platelet count was 422,400/cmm for the first group and 482,800/cmm for the second group.  (+info)

Enhanced myocardial glucose use in patients with a deficiency in long-chain fatty acid transport (CD36 deficiency). (2/11030)

CD36 is a multifunctional, 88 kDa glycoprotein that is expressed on platelets and monocytes/macrophages. CD36 also has high homology with the long-chain fatty acid (LFA) transporter in the myocardium. Although platelet and monocyte CD36 levels can indicate a CD36 deficiency, they cannot predict specific clinical manifestations in the myocardium of a given person. We examined the hypothesis that a deficiency in LFA transport augments myocardial glucose uptake in patients with a type I CD36 deficiency. METHODS: Seven fasting patients with a type I CD36 deficiency and 9 controls were assessed by cardiac radionuclide imaging using beta-methyl-p-iodophenyl-pentadecanoic acid (BMIPP) as a LFA tracer and by PET with 18F-fluorodeoxyglucose (FDG). RESULTS: None of the patients with a CD36 deficiency showed myocardial uptake of BMIPP. The percentage dose uptake of BMIPP in these subjects was significantly lower than that in normal controls (1.31+/-0.24 versus 2.90+/-0.2; P < 0.005). PET studies revealed that myocardial FDG accumulation was substantially increased in patients with a CD36 deficiency. Quantitative analysis showed that the percentage dose uptake of FDG in patients with a CD36 deficiency was significantly higher than that in normal controls (1.28+/-0.35 versus 0.43+/-0.22; P< 0.01). CONCLUSION: CD36 functions as a major myocardial LFA transporter and its absence may cause a compensatory upregulation of myocardial glucose uptake.  (+info)

Tyrosine phosphorylation of SLP-76 is downstream of Syk following stimulation of the collagen receptor in platelets. (3/11030)

Collagen-related peptide (CRP), a collagen homologue, induces platelet activation through a tyrosine kinase-dependent pathway, leading to sequential tyrosine phosphorylation of Fc receptor (FcR) gamma-chain, Syk, and phospholipase C-gamma2. Here we report that CRP and the platelet low affinity immune receptor FcgammaRIIA stimulate tyrosine phosphorylation of the T cell adapter SLP-76, whereas the G protein-coupled receptor agonist thrombin induces only minor tyrosine phosphorylation. This suggests that SLP-76 has a specific role downstream of receptors that signal via an immunoreceptor tyrosine-based activation motif. Immunoprecipitation studies demonstrate association of SLP-76 with SLAP-130, Vav, Fyn, Lyn, and the FcR gamma-chain in CRP-stimulated platelets. Several of these proteins, including SLP-76, undergo tyrosine phosphorylation in in vitro kinase assays performed on SLP-76 immunoprecipitates. Tyrosine phosphorylation of all of these proteins in the in vitro kinase assay was abrogated by the Src family kinase inhibitor PP1, suggesting that it is mediated by either Fyn or Lyn. The physiological significance of this is uncertain, however, since tyrosine phosphorylation of SLP-76 in vivo is not altered in either Fyn- or Lyn-deficient platelets. CRP stimulation of Syk-deficient platelets demonstrated that in vivo tyrosine phosphorylation of SLP-76 is downstream of Syk. The absence of Syk in the SLP-76 immunoprecipitates raises the possibility that another protein is responsible for bringing SLP-76 to Syk. Candidates for this include those proteins that co-immunoprecipitate with SLP-76, including the FcR gamma-chain. Tyrosine phosphorylation of PLC-gamma2 and Ca2+ mobilization is markedly attenuated in SLP-76-deficient platelets following CRP stimulation, suggesting that the adapter plays a critical role in the regulation of the phospholipase. The increase in tyrosine phosphorylation of SLAP-130 in response to CRP is also inhibited in SLP-76-deficient platelets, placing it downstream of SLP-76. This work identifies SLP-76 as an important adapter molecule that is regulated by Syk and lies upstream of SLAP-130 and PLC-gamma2 in CRP-stimulated platelets.  (+info)

Changes in haematological parameters and iron metabolism associated with a 1600 kilometre ultramarathon. (4/11030)

OBJECTIVE: To investigate haematological variations and iron related changes in the serum of participants in a 1600 kilometre ultramarathon run. PARTICIPANTS: Seven male and two female participants in a 1600 km foot race. METHODS: Blood samples were obtained from the participants before, after four and 11 days of running, and at the end of the event. Samples were analysed by standard methods for haemoglobin, packed cell volume, total red cell count, mean red cell volume, mean red cell haemoglobin, total white cell count and differential, platelets, reticulocytes, iron, ferritin, total iron binding capacity, percentage transferrin saturation, haptoglobin, and bilirubin and corrected for changes in plasma volume. RESULTS: The following variables decreased during the event (p < 0.05): haemoglobin, packed cell volume, mean red cell volume, percentage lymphocytes, percentage monocytes, serum iron, total iron binding capacity, and percentage transferrin saturation. Increases (p < 0.05) were found in plasma volume, total red cell count (day 4 only), total white cell count, percentage and absolute numbers of neutrophils and reticulocytes, absolute numbers of lymphocytes and monocytes (day 4 only), absolute numbers of eosinophils (day 11 and race end), absolute numbers of basophils (race end only), platelets, ferritin, haptoglobin, and bilirubin (day 4 only). CONCLUSION: Ultramarathon running is associated with a wide range of changes in haematological parameters, many of which are related to the normal acute phase response to injury. These should not be confused with indicators of disease.  (+info)

The Megakaryocyte/Platelet-specific enhancer of the alpha2beta1 integrin gene: two tandem AP1 sites and the mitogen-activated protein kinase signaling cascade. (5/11030)

The alpha2beta1 integrin, a collagen receptor on platelets and megakaryocytes, is required for normal platelet function. Transcriptional regulation of the alpha2 integrin gene in cells undergoing megakaryocytic differentiation requires a core promoter between bp -30 and -92, a silencer between bp -92 and -351, and megakaryocytic enhancers in the distal 5' flank. We have now identified a 229-bp region of the distal 5' flank of the alpha2 integrin gene required for high-level enhancer activity in cells with megakaryocytic features. Two tandem AP1 binding sites with dyad symmetry are required for enhancer activity and for DNA-protein complex formation with members of the c-fos/c-jun family. The requirement for AP1 activation suggested a role for the mitogen-activated protein kinase (MAPK) signaling pathway in regulating alpha2 integrin gene expression. Inhibition of the MAP kinase cascade with PD98059, a specific inhibitor of MAPK kinase 1, prevented the expression of the alpha2 integrin subunit in cells induced to become megakaryocytic. We provide a model of megakaryocytic differentiation in which expression of the alpha2 integrin gene requires signaling via the MAP kinase pathway to activate two tandem AP1 binding sites in the alpha2 integrin enhancer.  (+info)

Activation of stimulus-specific serine esterases (proteases) in the initiation of platelet secretion. I. Demonstration with organophosphorus inhibitors. (6/11030)

The effect of organophosphorus inhibitors of serine esterases (proteases) on secretion from washed rabbit platelets was examined. Five noncytotoxic stimuli were employed: collagen, thrombin, heterologous anti-platelet antibody (in the absence of complement), rabbit C3 bound to zymosan, and platelet activating factor derived from antigen-stimulated, IgE-sensitized rabbit basophils. Diisoprophyl phosphofluoridate, three series of p-nitrophenyl ethyl phosphonates, and a series of cyclohexyl phenylalkylphosphonofluridates were all found to be inhibitory to the platelet secretion. These are irreversible inhibitors of serine proteases but in this system were only inhibitory if added to the platelets concurrently with the stimuli. Pretreatment of either the platelets or the stimuli with the inhibitors followed by washing, was without effect on the subsequent reaction. This suggested the involvement of stimulus-activatable serine proteases in the secretory process. The concept was supported by finding that nonphosphorylating phosphonates or hydrolyzed phosphonates or phosphonofluoridates were without inhibitory action. The effect of a series of phosphonates or phosphonoflouridates in inhibiting each stimulus exhibited a unique activity-structure profile. The demonstration of such unique profiles with four series of inhibitors for each of the five stimuli was interpreted as demonstrating that a specific activatable serine protease was involved in the platelet secretory response to each stimulus.  (+info)

Glycoprotein (GP) Ib-IX-transfected cells roll on a von Willebrand factor matrix under flow. Importance of the GPib/actin-binding protein (ABP-280) interaction in maintaining adhesion under high shear. (7/11030)

Adhesion of platelets to sites of vascular injury is critical for hemostasis and thrombosis and is dependent on the binding of the vascular adhesive protein von Willebrand factor (vWf) to the glycoprotein (GP) Ib-V-IX complex on the platelet surface. A unique but poorly defined characteristic of this receptor/ligand interaction is its ability to support platelet adhesion under conditions of high shear stress. To examine the structural domains of the GPIb-V-IX complex involved in mediating cell adhesion under flow, we have expressed partial (GPIb-IX), complete (GPIb-V-IX), and mutant (GPIbalpha cytoplasmic tail mutants) receptor complexes on the surface of Chinese hamster ovary (CHO) cells and examined their ability to adhere to a vWf matrix in flow-based adhesion assays. Our studies demonstrate that the partial receptor complex (GPIb-IX) supports CHO cell tethering and rolling on a bovine or human vWf matrix under flow. The adhesion was specifically inhibited by an anti-GPIbalpha blocking antibody (AK2) and was not observed with CHO cells expressing GPIbbeta and GPIX alone. The velocity of rolling was dependent on the level of shear stress, receptor density, and matrix concentration and was not altered by the presence of GPV. In contrast to selectins, which mediate cell rolling under conditions of low shear (20-200 s-1), GPIb-IX was able to support cell rolling at both venous (150 s-1) and arterial (1500-10,500 s-1) shear rates. Studies with a mutant GPIbalpha receptor subunit lacking the binding domain for actin-binding protein demonstrated that the association of the receptor complex with the membrane skeleton is not essential for cell tethering or rolling under low shear conditions, but is critical for maintaining adhesion at high shear rates (3000-6000 s-1). These studies demonstrate that the GPIb-IX complex is sufficient to mediate cell rolling on a vWf matrix at both venous and arterial levels of shear independent of other platelet adhesion receptors. Furthermore, our results suggest that the association between GPIbalpha and actin-binding protein plays an important role in enabling cells to remain tethered to a vWf matrix under conditions of high shear stress.  (+info)

Activation of G12/G13 results in shape change and Rho/Rho-kinase-mediated myosin light chain phosphorylation in mouse platelets. (8/11030)

Platelets respond to various stimuli with rapid changes in shape followed by aggregation and secretion of their granule contents. Platelets lacking the alpha-subunit of the heterotrimeric G protein Gq do not aggregate and degranulate but still undergo shape change after activation through thromboxane-A2 (TXA2) or thrombin receptors. In contrast to thrombin, the TXA2 mimetic U46619 led to the selective activation of G12 and G13 in Galphaq-deficient platelets indicating that these G proteins mediate TXA2 receptor-induced shape change. TXA2 receptor-mediated activation of G12/G13 resulted in tyrosine phosphorylation of pp72(syk) and stimulation of pp60(c-src) as well as in phosphorylation of myosin light chain (MLC) in Galphaq-deficient platelets. Both MLC phosphorylation and shape change induced through G12/G13 in the absence of Galphaq were inhibited by the C3 exoenzyme from Clostridium botulinum, by the Rho-kinase inhibitor Y-27632 and by cAMP-analogue Sp-5,6-DCl-cBIMPS. These data indicate that G12/G13 couple receptors to tyrosine kinases as well as to the Rho/Rho-kinase-mediated regulation of MLC phosphorylation. We provide evidence that G12/G13-mediated Rho/Rho-kinase-dependent regulation of MLC phosphorylation participates in receptor-induced platelet shape change.  (+info)

Circulating platelets consist of subpopulations with different age, maturation state and size. In this review, we address the association between platelet size and platelet function and summarize the current knowledge on platelet subpopulations including reticulated platelets, procoagulant platelets and platelets exposing signals to mediate their clearance. Thereby, we emphasize the impact of platelet turnover as an important condition for platelet production in vivo. Understanding of the features that characterize platelet subpopulations is very relevant for the methods of platelet concentrate production, which may enrich or deplete particular platelet subpopulations. Moreover, the concept of platelet size being associated with platelet function may be attractive for transfusion medicine as it holds the perspective to separate platelet subpopulations with specific functional capabilities.
OBJECTIVE-The goal of this study was to specifically estimate the effectiveness of platelet releasate, a widely available treatment administered by a proprietary group of wound care centers (WCCs) for the treatment of diabetic neuropathic foot ulceration.. RESEARCH DESIGN AND METHODS-Treatment effectiveness was estimated in a retrospective cohort study controlling for treatment selection bias using logistic regression-derived propensity scores.. RESULTS-Platelet releasate was more effective than standard care. The relative risk for a wound to heal after treatment with platelet releasate compared with standard care at a WCC varied from 1.14 (95% CI 1.03-1.27) to 1.59 (1.49-1.70). The effect was greatest in those with the most severe wounds, i.e., large wounds that affect deeper anatomical structures.. CONCLUSIONS-Within the limitations of the ability of propensity score analysis to control for selection bias, platelet releasate is more effective than standard therapy. This effect is more ...
In this study, we show that the platelet surface expression of glycoprotein (GP) V is regulated by two independent mechanisms. While confirming that both thrombin and neutrophil elastase proteolyse GPV, we show that neutrophil cathepsin G, thrombin receptor activating peptide (TRAP), and a combination of ADP and epinephrine can each result in a decrease in the platelet surface expression of GPV by a nonproteolytic mechanism: a cytoskeletal-mediated redistribution of platelet surface GPV to the surface-connected canalicular system (SCCS). Four independent lines of evidence documented the nonproteolytic nature of this decrease in the platelet surface expression of GPV. First, flow cytometric studies showed that cathepsin G, TRAP, and ADP/epinephrine decreased the platelet surface expression of GPV without changing the total platelet content of GPV. Second, immunoelectron microscopy directly demonstrated translocation of GPV from the platelet surface to the SCCS. Third, the cathepsin G-, TRAP-, and ADP
TY - JOUR. T1 - Relationship between high platelet turnover and platelet function in high-risk patients with coronary artery disease on dual antiplatelet therapy. AU - Cesari, Francesca. AU - Marcucci, Rossella. AU - Caporale, Roberto. AU - Paniccia, Rita. AU - Romano, Eloisa. AU - Gensini, Gian Franco. AU - Abbate, Rosanna. AU - Gori, Anna Maria. PY - 2008/5. Y1 - 2008/5. N2 - A high platelet turnover rate produce a population of immature reticulated platelets (RP) that could confer, despite of antiplatelet drugs, a residual platelet reactivity (RPR) in coronary artery disease (CAD) patients. To assess the influence of RP on platelet reactivity in CAD patients on dual antiplatelet therapy we measured RP in 372 patients by using the Sysmex XE-2100 haematology analyzer and platelet function by optical platelet aggregometry (PA) on platelet-rich-plasma induced by 1 mmol arachidonic acid (AA-PA) and 10 μM ADP (ADP-PA). RPR was defined as either AA-PA ,20% or ADP-PA ,70%. RP were expressed as a ...
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TY - JOUR. T1 - Influence of rheologic changes and platelet-neutrophil interactions on cell filtration in sepsis. AU - Kirschenbaum, Linda A.. AU - Aziz, Mohammed. AU - Astiz, Mark E.. AU - Saha, Dhanonjoy C.. AU - Rackow, Eric C.. PY - 2000/1/1. Y1 - 2000/1/1. N2 - We examined the role of erythrocyte (red blood cell; RBC) aggregation and deformability, neutrophil (polymorphonuclear neutrophil; PMN) deformability, whole-blood viscosity, and platelet-neutrophil interactions on cell filtration in subjects who were critically ill with sepsis (CIS), critically ill noninfected subjects (CINS), and healthy controls (C). We assessed cell deformability by filtration through filters of 5-μm pore size. Whole blood, RBC, PMN, and combinations of PMN and RBC were studied. Viscometry was done on isolated RBC. Platelet-PMN interactions were assessed with monoclonal antibodies to CD41 and activated CD53 platelet receptors, and to CD66b PMN receptors. Filtration pressure (Pi) for CIS was significantly greater ...
Platelet membrane receptor P2Y12 H1/H2 polymorphisms is highly associated with cerebral infarction: a case-control study Shu-Jun Lu, Xiao-Sheng Zhou, Qi Zheng, Hong-Liang Chen, Yan-Lei Geng Department of Neurology, Binzhou Medical University Hospital, Binzhou, Peoples Republic of China Objectives: This study aimed to determine the relationship between the polymorphisms of the H1/H2 gene of platelet membrane receptor P2Y12 and cerebral infarction (CI) in a Han population in North Shandong Province, Peoples Republic of China. Patients and methods: A case-control study, which involved 168 nonstoke subjects (contrast group) and 152 CI patients (CI group), was conducted. The state of subjects in the CI group was validated by computed tomography or MRI. The clinical data were categorized into two groups. The data included age, gender, smoking, drinking, shrinkage pressure, diastolic blood pressure, blood glucose, cholesterol, triglyceride, low-density lipoprotein, high-density lipoprotein, serum uric
Partial purification and characterization of serine protease activity in platelets and platelet releasates from patients with Quebec platelet disorder Conference Paper ...
Approach and Results-To model standard antiplatelet therapy, platelets were treated in vitro with aspirin, the P2Y12 receptor blocker prasugrel active metabolite, and aspirin plus prasugrel active metabolite. Different proportions of uninhibited platelets were then introduced. Light transmission aggregometry analysis demonstrated clear positive associations between proportions of drug-free platelets and percentage platelet aggregation in response to a range of platelet agonists. Using differential platelet labeling coupled with advanced flow cytometry and confocal imaging, we found aggregates formed in mixtures of aspirin-inhibited platelets and drug-free platelets were characterized by intermingled platelet populations. This distribution is in accordance with the ability of drug-free platelets to generate thromboxane A2 and so drive secondary platelet activation. Conversely, aggregates formed in mixtures of prasugrel active metabolite-inhibited or aspirin plus prasugrel active ...
Introduction: S100A1 is a member of the S100 family of calcium-binding proteins. S100A1 controls Ca2+ dynamics in cardiomyocytes and plays an important role in heart failure. S100A1 is also strongly expressed in mouse platelets, but its role in platelet biology has not been investigated.. Goal: To determine the role of S100A1 in platelet activation and thrombosis.. Methods and Results: Platelet activation in response to threshold levels of convulxin, a specific agonist for the collagen receptor GPVI, showed significantly increased activation of αIIbβ3 integrin and α-granule release in S100A1-deficient (SKO) platelets compared with wild-type (WT) platelets. Consistently, SKO platelets also showed a more robust aggregation response to convulxin and collagen. In contrast, SKO platelets responded normally to stimulation with PAR4 receptor-activating peptide or ADP. Adhesion of SKO platelets to collagen under flow conditions was not significantly different to that of WT platelets. However, we ...
Oxidized LDL and platelets play a central role in the pathogenesis of atherosclerosis and ischemic cardiovascular diseases. Lysophosphatidic acid (LPA) is a thrombogenic substance that accumulates in mildly-oxidized LDL and in human atherosclerotic lesions, and is responsible for the initial platelet activation, shape change, induced by mildly-oxidized LDL and extracts of lipid-rich atherosclerotic plaques (Siess et al., 1999 Proc Natl Acad Sci USA 1999). LPA directly induced platelet shape change in blood and platelet-rich plasma (PRP) obtained from all blood donors. Albumin was one of the main inhibiting factors of platelet shape change in plasma. Interestingly LPA, at concentrations slightly above plasma levels, induced platelet shape change and aggregation in blood. 1-alkyl-LPA (16:0) was almost 20-fold more potent than 1-acyl-LPA (16:0). LPA-stimulated platelet aggregation in blood and PRP was donor-dependent. LPA-induced aggregation in blood could be completely blocked by the ADP- ...
A method for determining platelet activation by utilizing numeric counts of platelets before a sample of platelets has been activated and after the activatable platelets are activated with a platelet activation agonist and using the difference between such counts as an, indication of the platelet activity of the sample. There is also disclosed a method for using the electronic impedance cell counting technique for determining platelet activation wherein EDTA is used as a preservative by counting the platelets in an EDTA preserved sample using an electronic impedance cell counting technique and subtracting from that number the number of platelets remaining after the activatable platelets in a second sample have been activated with a platelet agonist in the absence of EDTA and using that difference as a measure of platelet activity.
Most heart attacks and strokes are caused by blood clots (thrombi) that block the vasculature. Because disease-causing arterial thrombosis depends on blood platelets, platelet inhibitors such as aspirin and clopidogrel effectively decrease the risk of thrombosis; however, they also impair platelet-dependent hemostasis that staunches bleeding from wounds and can therefore produce excessive bleeding. Experimental studies show that a reduction in the number of platelets also inhibits thrombosis, but these treatments also interfere with platelet function. Because normal hemostasis requires that the platelet concentration remain within a physiological range in the circulation, we evaluated whether lowering the number of circulating platelets-but only to a value still within the normal range-by inhibiting platelet formation in the bone marrow inhibits acute thrombogenesis in baboons. We reduced the platelet count with an inhibitor against the megakaryocyte-promoting hormone thrombopoietin and then ...
Platelets play a key and beneficial role for primary hemostasis on the disruption of the integrity of vessel wall. Platelet adhesion and activation at sites of vascular wall injury is initiated by adhesion to adhesive macromolecules, such as collagen and von Willebrand factor (vWF), or by soluble platelet agonists, such as ADP, thrombin, and thromboxane A2. Different receptors are stimulated by various agonists, almost converging in increasing intracellular Ca2+ concentration that stimulate platelet shape change and granule secretion and ultimately induce the inside-out signaling process leading to activation of the ligand-binding function of integrin alpha IIb beta 3. Binding of alpha IIb beta 3 to its ligands, mainly fibrinogen, mediates platelet adhesion and aggregation and triggers outside-in signaling, resulting in platelet spreading, additional granule secretion, stabilization of platelet adhesion and aggregation, and clot retraction ...
human platelet receptor for type III collagen: MW 68-72 kDa; platelet receptor involved in platelet interaction with type III collagen, localized within platelet lipid rafts where it could interact with other platelet receptors for collagen (GP VI and alpha2beta1 integrin) for efficient platelet activation
Reuters) - Scientists have for the first time created blood platelet cells by reprogramming stem cells derived from adult cells, offering the potential for a renewable supply of the fragile blood component.. Researchers at the Center for iPS Cell Research and Application at Kyoto University in Japan presented data here at a meeting of the American Society of Hematology showing they were able to create the cells in the laboratory and confirm they had the same life span as normal human platelets when infused in mice.. The next step will be to conduct a trial to determine whether our platelets can function in the human body and potentially provide a stable supply of platelets at a predefined quality and quantity that can then be used for transfusion therapy, D. Koji Eto, professor at the Kyoto center and senior author of the study, said in a statement.. Induced pluripotent stem cells, or iPS cells, are produced by manipulating ordinary human skin or blood cells back to a state in which they are ...
inbook{d2d8fceb-9b60-4916-ad95-3b1f6f9113f7, abstract = {,p,Many pathogenic bacteria have been reported to interact with human platelets to mediate platelet activation and aggregation. The importance of these interactions to the immune response or pathogenesis of bacterial infection has not been clarified. It may therefore be valuable to assess platelet responses mediated by diverse strains of bacteria. Here, I describe a method to study platelet integrin activation and granule release using flow cytometry, and a complementary method to study platelet aggregation using a dedicated platelet aggregometer. The combination of these methods represents a rapid and cost-effective strategy to provide mechanistic insight on the type of platelet response mediated by the bacteria.,/p,}, author = {Shannon, Oonagh}, issn = {10643745}, keyword = {Bacteria,Coagulation,Flow cytometry,Platelets,Streptococci}, language = {eng}, pages = {267--273}, publisher = {Humana Press}, series = {Methods in Molecular ...
The present study demonstrates that in type 2 DM patients with CAD receiving maintenance aspirin and clopidogrel therapy, the presence of moderate/severe CKD is associated with higher degrees of platelet reactivity compared with patients with normal renal function/mild CKD. In particular, after adjustment for potential confounders, patients with creatinine clearance ,60 ml/min had an almost 4-fold increase in the likelihood of showing high platelet reactivity after ADP stimuli and over a 2-fold increase in high platelet reactivity after collagen stimuli. Importantly, these patients with high platelet aggregability also have increased markers of platelet activation. Overall, these findings are indicative not only of dysfunctional purinergic signaling mediated ADP receptors but also of the presence of a hyper-reactive platelet phenotype with upregulation of multiple signaling pathways. Therefore, these pharmacodynamic observations might explain the elevated prevalence of ischemic complications, ...
OBJECTIVE-: Protease-activated receptor 4 (PAR4) is a key regulator of platelet reactivity and is encoded by F2RL3, which has abundant rare missense variants. We aimed to provide proof of principle that rare F2LR3 variants potentially affect on platelet reactivity and responsiveness to PAR1 antagonist drugs and to explore underlying molecular mechanisms. APPROACH AND RESULTS-: We identified 6 rare F2RL3 missense variants in 236 cardiac patients, of which the variant causing a tyrosine 157 to cysteine substitution (Y157C) was predicted computationally to affect most on PAR4 structure. Y157C platelets from 3 cases showed reduced responses to PAR4-activating peptide and to α-thrombin compared with controls, but no reduction in responses to PAR1-activating peptide. Pretreatment with the PAR1 antagonist vorapaxar caused lower residual α-thrombin responses in Y157C platelets than in controls, indicating greater platelet inhibition. HEK293 cells transfected with a PAR4 Y157C expression construct had ...
TY - JOUR. T1 - Four types of human platelet lysate, including one virally inactivated by solvent-detergent, can be used to propagate Wharton jelly mesenchymal stromal cells. AU - Chen, Ming Sheng. AU - Wang, Tsung Jen. AU - Lin, Hsiu Chen. AU - Thierry, Burnouf. PY - 2019/3/25. Y1 - 2019/3/25. N2 - There is accumulating experimental evidence that human platelet lysate (HPL) made from platelet concentrates can replace fetal bovine serum (FBS) as a xeno-free clinical-grade supplement of growth media to expand mesenchymal stromal cells (MSCs). However, uncertainties exist in regard to impacts that various manufacturing methods of HPL can exert on the expansion and differentiation capacity of MSCs. In particular, there is a need to evaluate the possibility of implementing virus-inactivation treatment during HPL production to ensure optimal safety of industrial HPL pools. Expired human platelet concentrates from four different donors were pooled and subjected to freeze-thaw cycles (-80/+37 °C), ...
The typical reconstruction model of an unstimulated human platelet is presented. It shows a strict spatial order of organelles and enodmembrane systems. The surface-connected vesicle system is predominantly found in the periphery. The dense tubules constitute a continuous, interconnected system found just under the surface of the platelet. They are particularly pronounced in the vicinity of the marginal microtubules. All organelles are in contact with the dense tubular system (DTS). Granules, vesicles and plasmalemma fuse into the tubules. The platelet organelles are centrally located. Lysosomal granules are primarily shperical and all of them contain a nucleoid. This speaks in favor of a single type of storage organelle in the platelet. The dense bodies and their remnants (large, solitary vesicles) are not joined by the other endomembrane systems, as in the case of the granules. A particular pathways for the release of substances from these organelles is now morphologically plausible. Mitochondria are
TY - JOUR. T1 - Platelet function after cardiac surgery and its association with severe postoperative bleeding. T2 - the PLATFORM study. AU - for The Surgical and Clinical Outcome Research (SCORE) Group. AU - Ranucci, Marco. AU - Pistuddi, Valeria. AU - Di Dedda, Umberto. AU - Menicanti, Lorenzo. AU - De Vincentiis, Carlo. AU - Baryshnikova, Ekaterina. PY - 2018/1/1. Y1 - 2018/1/1. N2 - Platelet dysfunction after cardiac surgery is a determinant of postoperative bleeding. The existing guidelines suggest the use of desmopressin and/or platelet concentrate transfusions in case of platelet dysfunction in bleeding patients, but no cut-off values for platelet activity exist in the literature. The Platelet Function in the Operating Room (PLATFORM) study aims to identify the relationship between platelet function after cardiopulmonary bypass and severe bleeding, finding adequate predictive values of platelet function for severe bleeding. The PLATFORM is a prospective cohort study on 490 adult patients ...
The frequency and severity of bacteremic infections has increased over the last decade and bacterial endovascular infections (i.e., sepsis or endocarditis) are associated with high morbidity and mortality. Bacteria or secreted bacterial products modulate platelet function and, as a result, affect platelet accumulation at sites of vascular infection and inflammation. However, whether bacterial products regulate synthetic events in platelets is not known. In the present study, we determined if prolonged contact with staphylococcal α-toxin signals platelets to synthesize B-cell lymphoma (Bcl-3), a protein that regulates clot retraction in murine and human platelets. We show that α-toxin induced αIIbβ3-dependent aggregation (EC50 2.98 µg/mL ± 0.64 µg/mL) and, over time, significantly altered platelet morphology and stimulated de novo accumulation of Bcl-3 protein in platelets. Adherence to collagen or fibrinogen also increased the expression of Bcl-3 protein by platelets. α-toxin altered Bcl-3
Methods were developed for measuring changes in platelet sensitivity to a release-inducing stimulus and in platelet cyclic AMP in fresh whole blood samples from rabbits. These techniques permitted detection of the effects of exogenous and endogenous prostacyclin on circulating platelets. In these methods, rabbit platelets were labeled in vitro by incubation with [14C]serotonin and [3H]adenine and then transfused into other rabbits. Release of platelet [14C]serotonin by a standard dose of synthetic platelet-activating factor (40 pmol/ml) and the platelet cyclic [3H]AMP levels were then measured in citrated blood from the conscious animals within 2 min of arterial puncture. Bolus intravenous injections of prostacyclin (1-10 nmol/kg) caused concentration-dependent increases in platelet cyclic AMP after 2 min, which decreased approximately 75% by 5 min, and disappeared after 30 min. Significant inhibition of the platelet release reaction was detected 2 min but not 5 min after injection of 10 nmol of ...
TY - JOUR. T1 - Transfection of human platelets with short interfering RNA. AU - Hong, Wei. AU - Kondkar, Altaf A.. AU - Nagalla, Srikanth. AU - Bergmeier, Wolfgang. AU - Jin, Ying. AU - Herman, Jay H.. AU - Bray, Paul F.. PY - 2011/6. Y1 - 2011/6. N2 - Platelets contain mRNAs and are capable of translating mRNA into protein, and it has been previously demonstrated that platelets increase their levels of integrin β3 overtime while in blood bank storage conditions. We are unaware of prior attempts to introduce nucleic acids into platelets. Considering the potential clinical and research utility of manipulating platelet gene expression, we tested whether small interfering RNAs (siRNAs) could be transfected into normal human platelets. Multiple conditions were tested, including lipofectamine versus electroporation, different amounts of siRNA, the effect of different buffers and the presence of plasma during transfection, and the time for optimal siRNA incorporation after transfection. Using flow ...
Release: Dec. 3, 1999. UI participates in multi-center study testing new platelet sterilizing strategy. IOWA CITY, Iowa The University of Iowa Hospitals and Clinics is participating in a multi-center trial testing the efficacy and safety of a new way to cleanse infectious organisms from donated platelets before the blood product is administered to patients.. The UI department of pathology has received a one-and-half-year grant from health care companies Baxter and Cerus for the study titled Determination of the therapeutic efficacy and safety of photochemically treated platelets in thrombocytopenic patients.. Thrombocytopenia is a condition characterized by low blood platelet counts. Platelets are the clotting particles in blood.. The new photochemical strategy attempts to sterilize donated platelets against bacteria and viruses, which may be present in blood products. The goal of the study is to establish that the platelets are not damaged from the sterilization process. Preliminary data ...
The TEMPLATE study design will enable unbiased comparison of the effects of TIC versus TIC + ASP on platelet activity in patients with coronary artery disease. It will also enable a longitudinal comparison of the effects TIC and TIC + ASP with the effects of ASP alone in the same patients. The laboratory tests selected for this study will enable measurement of functional platelet responses to a panel of activating agonists using LTA, flow cytometry and flow chamber tests, selected to measure the extent of inhibition of the multiple platelet activation pathways. We will also measure the extent of baseline platelet activity by testing unstimulated platelets by flow cytometry and with the soluble platelet activation biomarker tests. Together, these data will provide a comprehensive description of the overall pharmacodynamic effects of the different antiplatelet treatments. This information has not been reported previously in cohorts of patients with coronary artery disease receiving TIC or TIC + ...
Blood platelets commonly called megakaryocytes are one of the three tiny cellular components of the blood which helps in the stoppage of bleeding.. Other cellular components of blood are the red blood cells and white blood cells. All the cellular components bathe in the plasma.. Nearly half a billion blood platelets are being formed in the bone marrow every day. These play a primary role in the formation of blood clot. Here I would like to define the two common terms related to the platelet plug formation when the blood vessel in injured.. * Adhesion means sticking of blood platelets with the wall of the bleeding vessel.. * Aggregation means sticking of the platelets with each other.. Normally, in the circulating blood, platelets keep on flowing without any adhesion or aggregation. However, injury to the wall of blood vessel is the point when blood platelets start clinging to the injured part of the vessel wall. At the same time the platelets aggregate with one another and completely seal the ...
The normal circulating platelet count is maintained within relatively narrow limits (150,000-450,000 platelets/μL in Northern Europeans and 90,000-300,000 platelets/μL in people of Mediterranean descent). This difference is related to an inherited slight variation in individual platelet volume (size). The platelet volume is inversely related to the platelet count, so the mass of circulating platelets is the same for these 2 populations. Approximately one-third of platelets are sequestered in the spleen at any one time. Splenic sequestration of platelets can increase dramatically with splenomegaly. Since a platelet has a lifespan of approximately 9-10 days, some 15,000-45,000 platelets/μL must be produced each day to maintain a steady state. New platelet production is the responsibility of the megakaryocyte, a very large multinucleated cell (10,750 fL) found in relatively small numbers in the marrow (0.1% of marrow cells) (Figure 31-1). As with other hematopoietic cells, megakaryocytes are ...
Research in the Laboratory for Hemostasis and Platelet Biology, led by Dr. Andrew Johnson, focuses on understanding genetic and genomic underpinnings of this individual variability in therapeutically targeted CVD pathways ...
Although tumor dormancy is highly prevalent, the underling mechanisms are still mostly unknown. It is unclear which lesions will progress and become a disseminated cancer, and which will remain dormant and asymptomatic. Yet, an improved ability to predict progression would open the possibility of timely treatment and improvement in outcomes. We have recently described the ability of platelets to selectively uptake angiogenesis regulators very early in tumor growth, and proposed their use as an early marker of malignancy. In this review we will summarize current knowledge about these processes and will discuss the possibility of using platelet content to predict presence of occult tumors.
OBJECTIVES: We sought to examine whether patients with stable coronary artery disease (CAD) have increased platelet reactivity and an enhanced propensity to form monocyte-platelet aggregates. BACKGROUND: Platelet-dependent thrombosis and leukocyte infiltration into the vessel wall are characteristic cellular events seen in atherosclerosis. METHODS: Anticoagulated peripheral venous blood from 19 patients with stable CAD and 19 normal control subjects was incubated with or without various platelet agonists and analyzed by whole blood flow cytometry. RESULTS: Circulating degranulated platelets were increased in patients with CAD compared with control subjects (mean [+/- SEM] percent P-selectin-positive platelets: 2.1 +/- 0.2 vs. 1.5 +/- 0.2, p andlt; 0.01) and were more reactive to stimulation with 1 micromol/liter of adenosine diphosphate (ADP) (28.7 +/- 3.9 vs. 16.1 +/- 2.2, p andlt; 0.01), 1 micromol/liter of ADP/epinephrine (51.4 +/- 4.6 vs. 37.5 +/- 3.8, p andlt; 0.05) or 5 micromol/liter of thrombin
Our data clearly show that human platelets can both bind and degrade full-length rhTPO. The affinity constants derived from the binding data indicate that rhTPOs affinity for platelet receptors was similar to that for the cloned c-Mpl receptor construct, gD-Mpl. The binding constants were also similar for rhTPO binding to PRP at 37°C and WP at 22°C. These data show that platelets bind rhTPO, likely via c-Mpl with high affinity (∼350 pmol/L), and that this binding is saturable. Scatchard analysis predicted a low number of (∼23 to 224) binding sites per platelet. However, this estimate may be low, because c-Mpl binding sites on circulating platelets are likely partially occupied by endogenous circulating TPO.9,10 If we assume the number of available c-Mpl receptors is ∼25 to 200 per platelet and that the approximate number of platelets per liter of blood in humans is 200 × 109, then the predicted binding capacity would be ∼8 to 64 pmol per liter of blood. This approximation suggests ...
Subjects for all study groups will be male and between the ages of 25 and 65. Hypertension & Depression Group: Hypertension controlled with an ACE-inhibitor anti-hypertensive; no co-morbid medical conditions known to influence psychological functioning or platelet calcium responses including uncontrolled diabetes, MI or CVA within 6 months of enrollment, secondary hypertension; depression as diagnosed by structured interview and HDRS score of 18; no active participation in another clinical trial; no current suicidal/ homicidal ideation. Hypertension Group: Hypertension controlled with an ACE-inhibitor anti-hypertensive; no co-morbid medical conditions known to influence psychological functioning or platelet calcium responses including uncontrolled diabetes, MI or CVA within 6 months of enrollment, secondary hypertension; no active participation in another clinical trial; no current suicidal/ homicidal ideation. Depression Group: No co-morbid medical conditions known to influence psychological ...
Platelets may interact directly with their targets and perform its killing function. For example, platelets may bind and wrap bacteria (Youssefian et al., 2002) or induce their aggregation (OBrien et al., 2002), leading to degranulation. During malaria infection, platelets have also been described to perform the direct killing of plasmodium parasites in their blood stage forms in a PF4-dependent manner (McMorran et al., 2009, 2012), leading to the general perception that platelets play protective roles during an infection. However, a recent in vivo study in mice paradoxically found that platelet depletion did not lead to higher parasitemia levels (Gramaglia et al., 2017). Instead, links were found between the presence of platelets and malarial pathogenesis via CD40 interactions. Because about two thirds (Jadhav et al., 2004) of malarial infections are accompanied by thrombocytopenia, it thus remains a quandary for clinicians to decide if they should be boosting or inhibiting platelet function ...
1. A new fixing solution is described, which preserves the platelets and prevents contact hemolysis of the erythrocytes, so that counts of both corpuscles may be made in the same preparation.. 2. Comparative counts of platelets in arteries and veins show that arterial blood contains a larger number of platelets than venous blood. This difference is accentuated under experimental conditions that cause a reduction in the number of platelets. It is concluded that new platelets are added to the blood in the capillary areas of the lungs, and that there is a corresponding destruction of platelets as the blood passes through the capillary areas of the systemic circulation.. 3. Perfusion of the lungs with a platelet-preserving solution, compared with that of other organs, gives evidence of the existence of a source of platelet material in the lungs.. 4. Histological examination of the lungs with a technique adequate to give a differential staining of platelet material demonstrates the presence of giant ...
New studies in mice suggest that blood platelets can destroy deadly malaria parasites, but a single dose of aspirin may be enough to thwart their killing power., , , , The findings could have important im...
ANTICOAGULANTS AND THROMBOLYTIC DRUGSHaemostasisVascular injury results firstly in vasoconstriction and formation of platelet plug at the site of injury (primary haemostasis). The platelet plug is then stabilized by the formation of a fibrin meshwork, resulting from activation of the coagulation cascade.Fibrin is eventually cleared through digestion by fibrinolytic enzymes.Primary HaemostasisWhen endothelial integrity is breached, platelets adhere to exposed subendothelial collagen. The adherent platelets become activated result in;1) Exposure of fibrinogen receptors, allowing fibrinogen to bind and cross-link adjacent platelets. The process is known as platelet aggregation. The platelet fibrinogen receptor consists of a complex of glycoproteins IIb and IIIa on the platelet membrane.2) Release of contents of secretory granules including substances such as adenosine diphosphate (ADP) which promote further platelet activation.3) Synthesis of thromboxane A2 which also acts to promote further ...
AimsTo assess whether platelet reactivity is increased in offspring of patients with early acute myocardial infarction (AMI) and its possible relation with endothelial dysfunction.Methods and resultsWe studied 23 healthy children (15 ± 3 years, 13 males) of patients with early AMI (≤50 years old; Group 1) and 21 healthy children of healthy subjects without any history of cardiovascular disease (14 ± 3 years, 10 males; Group 2). Platelet reactivity was assessed by flow cytometry as the increase in monocyte-platelet aggregates (MPA) and CD41 and PAC-1 platelet expression in response to exercise stress test (EST), adenosine diphosphate (ADP) stimulation (10 -7 M), or both. Endothelial function was assessed by measuring brachial artery dilation during post-ischaemic forearm hyperaemia [flow-mediated dilation (FMD)]. Both EST and ADP induced a higher percentage increase in platelet receptor expression in Group 1, compared with Group 2, with the most significant difference being shown for the ...
As conventional tissue biopsies have several drawbacks, much effort has been directed toward the development of minimal-invasive liquid biopsy platforms for detecting and profiling cancer.. Platelets are the second most abundant cells in blood and have very versatile functions both in physiological and pathophysiological conditions. When exposed to tumors and their environment, platelets exchange biomolecules with tumor cells changing the platelets RNA profile, resulting in tumor-mediated education of the platelets. Our research group and collaborators have previously shown that platelets sequester material while in circulation and with that ability accumulate cancer specific information. Platelet RNA profiles or detection of tumor-derived biomarkers within them may provide insight into ongoing cancer-related processes in a patient, allowing for implementation of personalized therapy strategies.. This thesis evaluates whether circulating platelets could have a potential role (as a liquid biopsy ...
Platelets, also called thrombocytes (thromb- + -cyte, blood clot cell), are a component of blood whose function (along with the coagulation factors) is to stop bleeding by clumping and clotting blood vessel injuries. Platelets have no cell nucleus: they are fragments of cytoplasm that are derived from the megakaryocytes of the bone marrow, and then enter the circulation. These unactivated platelets are biconvex discoid (lens-shaped) structures, 2-3 µm in greatest diameter. Platelets are found only in mammals, whereas in other animals (e.g. birds, amphibians) thrombocytes circulate as intact mononuclear cells. On a stained blood smear, platelets appear as dark purple spots, about 20% the diameter of red blood cells. The smear is used to examine platelets for size, shape, qualitative number, and clumping. The ratio of platelets to red blood cells in a healthy adult is 1:10 to 1:20. The main function of platelets is to contribute to hemostasis: the process of stopping bleeding at the site of ...
After deposition of a platelet monolayer over the exposed VWF and collagen, the next step required for thrombus formation is the recruitment of additional platelets from the flowing blood, which upon activation acquire the ability to stick to each other in a process commonly referred to as platelet aggregation. This is made possible by the local accumulation of soluble agonists that are secreted/produced by adherent-activated platelets, including ADP, TxA2, epinephrine and thrombin. The final step is activation of αIIbβ3, causing a conformational change that enables it to bind fibrinogen and VWF, allowing stable bridges between platelets. The great number of αIIbβ3 copies on the platelet surface, (40,000-80,000), allows the assembly of large aggregates at the site of vascular injury. Activation of αIIbβ3 integrin requires agonist-driven activation events in recruited platelets, referred to as inside-out signaling, including the sequential activation of one or more PLC isoforms yielding a ...
Fig.10 Design of CREKA-Lipo-T nanoparticles and their proposed antimetastatic mechanism within tumor tissues. (A) Proposed mechanism of action of CREKA-Lipo-T nanoparticles. Normally, tumor growth factor (TGF)-b secreted by platelets induces transition of tumor cells to a mesenchymal-like phenotype (I). Platelets can also protect tumor cells against attack from natural killer (NK) cells (II). At distant sites, platelets assist metastatic cells to cross the local endothelium by secreting numerous cytokines. Following treatment, CREKA-Lipo-T actively targets microthrombi in tumor vessel walls and releases ticagrelor slowly and locally. Ticagrelor binds to tumor-associated platelets and inhibits their functions. The release of TGF-b from platelets and the interaction between platelets and tumor cells are abolished, leading to decreased epithelial-mesenchymal-like transition of tumor cells and thus inhibiting their invasion capacity. When tumor cells are present in circulation, compromised platelets ...
TY - JOUR. T1 - Preferential binding of platelets to monocytes over neutrophils under flow. AU - Ahn, Kyung C.. AU - Jun, Andy J.. AU - Pawar, Parag. AU - Jadhav, Sameer. AU - Napier, Susan. AU - McCarty, Owen J.T.. AU - Konstantopoulos, Konstantinos. PY - 2005/4/1. Y1 - 2005/4/1. N2 - This study was undertaken to systematically investigate the binding kinetics of platelet recruitment by monocytes relative to neutrophils in bulk suspensions subjected to shear as well as the molecular requirements of leukocyte-platelet binding. Hydrodynamic shear-induced collisions augment the proportion of monocytes with adherent platelets more drastically than that of neutrophils with bound platelets. These heterotypic interactions are further potentiated by platelet activation with thrombin or to a lesser extent by monocyte stimulation with N-formyl-methionyl-leucyl-phenylalanine (fMLP). Monocyte-platelet heteroaggregation increases with increasing shear rate and shear exposure time. Platelet P-selectin ...
Cooper, N., Heddle, N. M., de Haas, M., Reid, M. E., Lesser, M. L., Fleit, H. B., Woloski, B. M. R. and Bussel, J. B. (2004), Intravenous (IV) anti-D and IV immunoglobulin achieve acute platelet increases by different mechanisms: modulation of cytokine and platelet responses to IV anti-D by FcγRIIa and FcγRIIIa polymorphisms. British Journal of Haematology, 124: 511-518. doi: 10.1111/j.1365-2141.2004.04804.x ...
Tortuous blood vessels are often seen in humans in association with thrombosis, atherosclerosis, hypertension, and aging. Vessel tortuosity can cause high fluid shear stress, likely promoting thrombosis. However, the underlying physical mechanisms and microscale processes are poorly understood. Accordingly, the objectives of this study were to develop and use a new computational approach to determine the effects of venule tortuosity and fluid velocity on thrombus initiation. The transport, collision, shear-induced activation, and receptor-ligand adhesion of individual platelets in thrombus formation were simulated using discrete element method. The shear-induced activation model assumed that a platelet became activated if it experienced a shear stress above a relative critical shear stress or if it contacted an activated platelet. Venules of various levels of tortuosity were simulated for a mean flow velocity of 0.10 cm s−1 , and a tortuous arteriole was simulated for a mean velocity of 0.47 ...
Article see p 476. As megakaryocytes form platelets, they transfer STAT3 to proplatelet tips. Consequently, STAT3 is found in platelets that circulate in the bloodstream (Figure). The presence of STAT3 in platelets raises the question of whether it regulates functional responses in platelets or is simply a vestigial remnant of megakaryocytes. An argument for the leftover without function hypothesis is the anucleate status of platelets: simply stated, with no nucleus and no nuclear DNA there is no place for STAT3 to stick in platelets. The problem with this argument is that simple is no longer a common word used to describe platelets. Moreover, why would platelets expend energy to carry a protein that they do not need, especially since previous studies have shown that STAT3 undergoes signal-dependent phosphorylation in these anucleate cytoplasts?4 Well, any doubt regarding why STAT3 is present in platelets has been cleared up. Using a combination of pharmacological and genetic based tools, ...
Background: Coated-platelets, a subset of activated platelets observed with dual-agonist stimulation with collagen and thrombin, represent 30% of the platelet population in normal controls. In recently published work, we have shown that elevated coated-platelet levels (,45%) are predictive of stroke in asymptomatic carotid stenosis. We now investigate if platelet count and mean platelet volume (MPV) are related to coated-platelet levels.. Methods: Coated-platelet levels were measured in a cohort of asymptomatic outpatients referred for carotid ultrasound studies. Platelet count and mean platelet volume for each subject were recorded from the VA electronic medical record at the closest possible time period (within ≤6 months) to the date of coated-platelet sample. Correlations between each parameter and coated-platelet levels were determined and those reaching significance at p≤0.1 were included in a multiple regression model with LDL and systolic blood pressure (SBP), variables previously ...
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TY - JOUR. T1 - A study of whole blood platelet and white cell aggregation using a laser flow aggregometer. AU - Sun, J.. AU - Abel, E. W.. AU - Bancroft, A.. AU - McLaren, M.. AU - Belch, J. J. F.. PY - 2003. Y1 - 2003. N2 - Both platelet aggregation and white blood cell aggregation are involved in pathological processes such as thrombosis, atherosclerosis and chronic inflammation. People in older age groups are likely to suffer from cardiovascular diseases and may have increased white cell and platelet aggregation which could contribute to this increased risk. This study aimed to compare white cell and platelet aggregation between different age and gender groups. Whole blood white cell aggregation and platelet aggregation were carried out on healthy volunteers using cytometric techniques. It was found that both white cell and platelet aggregation in the elderly group (white cell aggregation median value, 0.08; range, 0.02-0.14; platelet aggregation median value, 0.32; range, 0.1-0.39) were ...
Systematic review (Open Access) on the comparison of different platelet transfusion thresholds prior to insertion of central lines in patients with thrombocytopenia from Cochrane Database of Systematic Reviews Comparison of different platelet transfusion thresholds prior to insertion of central lines in patients with thrombocytopenia #vascularaccess #FOAMva #FOAMed #FOAMcc #POCUS #patientsafety
Megakaryocytes generate platelets by remodeling their cytoplasm into long proplatelet extensions, which serve as assembly lines for platelet production. Although the mechanics of proplatelet elongation have been studied, the terminal steps of proplatelet maturation and platelet release remain poorly understood. To elucidate this process, released proplatelets were isolated, and their conversion into individual platelets was assessed. This enabled us to (a) define and quantify the different stages in platelet maturation, (b) identify a new intermediate stage in platelet production, the preplatelet, (c) delineate the cytoskeletal mechanics involved in preplatelet/proplatelet interconversion, and (d) model proplatelet fission and platelet release. Preplatelets are anucleate discoid particles 2-10 \(\mu\)m across that have the capacity to convert reversibly into elongated proplatelets by twisting microtubule-based forces that can be visualized in proplatelets expressing GFP-\(\beta\)1-tubulin. The ...
In patients with thrombocytopenia, it can be difficult to predict a patients bleeding risk based on platelet count alone. Platelet reactivity may provide additional information; however, current clinical assays cannot reliably assess platelet function in the setting of thrombocytopenia. New methods to study platelet reactivity in thrombocytopenic samples are needed. In this study, we sought to develop a laboratory model of thrombocytopenia using blood from healthy subjects that preserves the whole blood environment and reproducibly produces samples with a specific platelet count and hematocrit. We compared the activation state of unstimulated and agonist-stimulated platelets in thrombocytopenic samples derived from this method with normocytic controls. Whole blood was diluted with autologous red blood cell concentrate and platelet-poor plasma, which were obtained via centrifugation, in specific ratios to attain a final sample with a predetermined platelet count and hematocrit. P-selectin exposure and
TY - JOUR. T1 - Functional alterations of human platelets following 111In labeling with different ligands and incubation media. AU - Mieno, M.. AU - Isaka, Y.. AU - Kimura, K.. AU - Matsumoto, M.. AU - Etani, H.. AU - Uehara, A.. AU - Hashikawa, K.. AU - Hata, R.. AU - Moriwaki, H.. AU - Ashida, K.. AU - Imaizumi, M.. AU - Kamada, T.. AU - Kozuka, T.. PY - 1990/1/1. Y1 - 1990/1/1. N2 - We studied the effects of various 111In-water soluble chelates and incubation media on labeling efficiency of platelets and in vitro platelet aggregability. High labeling efficiency of platelets in ACD-saline was achieved with 111In-oxine sulfate, 111In-tropolone and 111In-MPO (2-mercaptopyridine-N-oxide). In the condition with 4.8 x 106/mm3 platelets in ACD-plasma, 111In-oxine-sulfate had low labeling efficiency and inconsistent labeling, while 111In-tropolone and 111In-MPO had high labeling efficiency. In vitro platelet aggregability (ADP 2 μM) was reduced when platelets were labeled in the absence of plasma. ...
With platelet activation, there is modulation of platelet surface molecule expression. In flow cytometric analyses of in vivo platelet activation, results are often confounded by activation induced in vitro by the preparative procedures. It is particularly important therefore to prevent or retard platelet activation as soon as possible after withdrawal of the blood sample. Taking blood into paraformaldehyde, or fixing the cells with paraformaldehyde as soon as possible after withdrawal, has been employed to prevent platelet activation in vitro, but paraformaldehyde-fixed platelets cannot be further used in functional studies. We investigated the efficacy of Diatube-H, a commercially available combination of platelet antagonists (theophylline, adenosine, and dipyridamole), in preventing or retarding platelet activation in vitro, along with its effects on modulation of platelet membrane glycoproteins (GP) and adhesion molecules. In contrast to blood taken into EDTA, blood taken into Diatube-H ...
The present study clarifies several aspects of the effects on platelet aggregation of arising and assuming the upright posture in the morning: 1) On arising, increased platelet aggregation can readily be observed in whole blood; 2) this increased aggregation is not accompanied by platelet activation, as evidenced by changes in activation-dependent markers on the platelet surface; 3) the observed increase in aggregation in whole blood may be partly explained by increases in platelet count and hematocrit that accompany arising. In addition, the study confirmed previous reports of increased fibrinolysis on standing and provided new evidence of an opposing increase in thrombin generation on standing.. Comparison with previous studies. Studies reporting the effects of arising in the morning on platelet aggregation (7-11,13) have exclusively studied aggregation in PRP, and to our knowledge, the present study is the first to report the effects of arising on platelet aggregation in whole blood. Of the ...
FIG. 2. Cleavage of α2-8-linked sialic acid from platelet surfaces results in a reduction of S. mitis binding. Binding of SF100 (□) to untreated human platelets (lane 1) or platelets pretreated with sialidase A (lane 2), sialidase V (lane 3), or sialidase C (lane 4). Binding of PS344 (▪) to platelets (lane 5) or platelets pretreated with sialidase A (lane 6), sialidase V (lane 7), or sialidase C (lane 8). Values presented are expressed as a percentage of wild-type binding to untreated platelets (mean ± SD). Data represent three experiments performed in triplicate on different occasions from a different donor each time. Note that these donors are different from those shown in Fig. 1. ...
Normal primary platelet aggregation requires agonist-mediated activation of membrane GPIIb-IIIa, binding of fibrinogen to GPIIb-IIIa, and cellular events after ligand binding. PAC1 monoclonal antibody distinguishes between resting and activated states of GPIIb-IIIa, and other antibodies preferentially recognize GPIIb (PMI-1) or IIIa (anti-LIBS1) after the binding of fibrinogen or fibrinogen-mimetic peptides, such as GRGDSP. Using these antibodies and platelet flow cytometry, we studied two distinct persistent platelet aggregation abnormalities. Platelets from a thrombasthenic variant, which contained near-normal amounts of GPIIb-IIIa, failed to aggregate or bind PAC1 in response to agonists. In addition, GRGDSP, which binds to normal GPIIb-IIIa without prior cell activation, failed to increase the binding of PMI-1 or anti-LIBS1 to the thrombasthenic platelets, suggesting a primary defect in ligand binding. Chromatography of detergent-solubilized platelets on a KYGRGDS affinity column confirmed ...
Platelets are cell fragments which are mainly an essential component when it comes to blood clotting and coagulation. The platelets along with the red blood cells and white blood cells, function together for the regulation and normal functioning of blood in our body. The blood cells have each of their own different life span as well as different functions. Among these three, the platelets are the smallest but one of the most important factors in blood clotting. If the platelets dont get regulated properly, such as when they dont get renewed at normal intervals, then the blood dysfunction or disorder might occur.. The general lifespan of a platelet is about 10 days. The normal platelet count in the human blood ranges from 150,000 to 450,000 per micro liter of blood. The platelet count varies in different locations and organs. But in cases of medical conditions, the platelets count may either increase or decrease in the blood. One of the most common condition of platelet count changes is the ...
SUMMARY Acquired qualitative platelet disorders are frequent causes of abnormal platelet function measured in vitro, although by themselves are usually associated with little or no clinical bleeding. However, there are important exceptions. Nevertheless, their major clinical impact becomes apparent in the additional presence of thrombocytopenia, or additional acquired or congenital disorders of hemostasis. Acquired disorders of platelet function can be conveniently classified into those that result from drugs, hematologic diseases, and systemic disorders. Drugs are the most frequent cause of acquired qualitative platelet dysfunction. Aspirin is the most notable drug in this regard because of its frequent use, its irreversible effect on platelet prostaglandin synthesis, and its documented effect on hemostatic competency, although this effect is minimal in normal individuals. Other nonsteroidal antiinflammatory drugs reversibly inhibit platelet prostaglandin synthesis and usually have little ...
Platelets are an important blood component that helps in controlling bleeding. The most abundant particle in our blood is the platelet that causes the blood to clot whenever theres a cut or injury, thereby stopping the bleeding in minutes. Now, there are tens and thousands of platelets in normal which help in this process. But in cases of certain diseases and illness like dengue etc the platelets gets destroyed by the infective germ, making the body prone to bleeding. Such a low count of platelet can cause serious brain damage and other organ damage. Apart from a blood transfusion, the most trusted way to increase your platelet count is by eating low platelets treatment food. Foods to increase platelet count are often used as basic preventive care and home remedy for diseases like dengue. But how do these home remedies to increase platelets work is a very basic understanding of…. ...
A low blood platelet count, known as thrombocytopenia, prevents blood from clotting normally. This can be an indication of serious medical conditions.SignificanceThe three main reasons for a low blood platelet count include reduced platelet production at bone marrow, blockage of platelets at enlarged spleen and an overuse or elimination of platelets by the body.Conditions Affecting Bone MarrowHIV and cancers such as leukemia and lymphoma reduce the capacity of bone marrow to produce platelets.Conditions Affecting the SpleenMyelofibrosis and certain forms of cirrhosis can enlarge the spleen. This hinders the passage of platelets into the bl...
While the heterozygous Q43P β1-tubulin carriers have a reduced function, the β1-tubulin-deficient mice present with only minor abnormalities in platelet hemostatic functions. Besides the fact that human platelets are more easy to handle and study in detail than mouse platelets, this can probably be explained by the fact that the loss of β1-tubulin expression in mouse platelets was overcome by overexpression of the other platelet β-tubulin variants,7 while the Q43P carrier platelets not only show reduced β1-tubulin but also total β-tubulin protein levels. In addition, incorporation of GFP-tagged Q43P β1-tubulin into wild-type tubulin structures seems to be inefficient and delocalized.. Due to the platelet dysfunction phenotype, the Q43P β1-tubulin variant could not only be conceived as a genetic risk factor for the development of thrombocytopenia but also as a protective genetic factor against cardiovascular disease. Indeed, a case-control study showed that the prevalence of Q43P ...
Activated blood platelets mediate the primary response to vascular injury. Although molecular abnormalities of platelet proteins occur infrequently, taken collectively, an inherited platelet defect accounts for a bleeding diathesis in ≈1:20,000 individuals. One rare example of a platelet disorder, Glanzmann thrombasthenia (GT), is characterized by life-long morbidity and mortality due to molecular abnormalities in a major platelet adhesion receptor, integrin αIIbβ3. Transfusion therapy is frequently inadequate because patients often generate antibodies to αIIbβ3, leading to immunemediated destruction of healthy platelets. In the most severe cases allogeneic bone marrow transplantation has been used, yet because of the risk of the procedure it has been limited to few patients. Thus, hematopoietic stem cell gene transfer was explored as a strategy to improve platelet function within a canine model for GT. Bleeding complications necessitated the use of a mild pretransplant conditioning ...
Significance: Levels of platelet noncoding RNAs (ncRNAs) are altered by disease, and ncRNAs may exert functions inside and outside of platelets. Their role in physiologic hemostasis and pathologic thrombosis remains to be explored. Recent Advances: The number of RNA classes identified in platelets has been growing since the past decade. Apart from coding messenger RNAs, the RNA landscape in platelets comprises ncRNAs such as microRNAs, circular RNAs, long ncRNAs, YRNAs, and potentially environmentally derived exogenous ncRNAs. Recent research has focused on the function of platelet RNAs beyond platelets, mediated through protective RNA shuttles or even cellular uptake of entire platelets. Multiple studies have also explored the potential of platelet RNAs as novel biomarkers. Critical Issues: Platelet preparations can contain contaminating leukocytes. Even few leukocytes may contribute a substantial amount of RNA. As biomarkers, platelet RNAs have shown associations with platelet activation, but ...
Activation of platelets with thrombin and other agonists causes a rapid increase in the phosphorylation of multiple proteins on tyrosine. To identify candidate protein-tyrosine kinases (PTKs; EC that may be responsible for these phosphorylation events, we analyzed the expression of seven Src-family PTKs and examined the association of these kinases with known platelet membrane glycoproteins. Five Src-related PTKs were detected in platelets: pp60SRC, pp60FYN, pp62YES, pp61HCK, and two LYN products of Mr 54,000 and 58,000. The Fgr and Lck PTKs were not detected. Although strict comparative quantification of protein levels was not possible, pp60SRC was detected at higher levels than any of the other kinases. In addition, glycoprotein IV (GPIV, CD36), one of the major platelet membrane glycoproteins, was associated in a complex with the Fyn, Yes, and Lyn proteins in platelet lysates. Similar complexes were also found in two GPIV-expressing cell lines, C32 melanoma cells and HEL cells. ...
Phosphatidylinositol 3-kinaseβ (PI3Kβ) plays a predominant role in integrin outside-in signaling and in platelet activation by GPVI engagement. We have shown that the tyrosine kinase Pyk2 mediates PI3Kβ activation downstream of integrin αIIbβ3, and promotes the phosphorylation of the PI3K-associated adaptor protein c-Cbl. In this study, we compared the functional correlation between Pyk2 and PI3Kβ upon recruitment of the two main platelet collagen receptors, integrin α2β1 and GPVI. PI3Kβ-mediated phosphorylation of Akt was inhibited in Pyk2-deficient platelets adherent to monomeric collagen through integrin α2β1, but occurred normally upon GPVI ligation. Integrin α2β1 engagement led to Pyk2-independent association of c-Cbl with PI3K. However, c-Cbl was not phosphorylated in adherent platelets, and phosphorylation of Akt occurred normally in c-Cbl-deficient platelets, indicating that the c-Cbl is dispensable for Pyk2-mediated PI3Kβ activation. Stimulation of platelets with CRP, a ...
TY - JOUR. T1 - Potentiation of TRAP-6-induced platelet dense granule release by blockade of P2Y12 signaling with MRS2395. AU - Mitrugno, Annachiara. AU - Rigg, Rachel A.. AU - Laschober, Nicole B.. AU - Ngo, Anh T.P.. AU - Pang, Jiaqing. AU - Williams, Craig D.. AU - Aslan, Joseph E.. AU - McCarty, Owen J.T.. N1 - Funding Information: This work was supported by grants from the National Institutes of Health (R01HL101972 and R01GM116184 to O.J.T.M.), the American Heart Association (13EIA12630000 to O.J.T.M. and 17SDG33350075 to J.E. A.) and the Altarum Institute (C.D.W. and O.J.T.M.). N.B.L. is a Johnson scholar. R.A.R is a Whitaker International Scholar. Funding Information: Super resolution microscopy studies were supported in part by the M.J. Murdock Charitable Trust. Publisher Copyright: © 2018 Taylor & Francis. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.. PY - 2018/5/19. Y1 - 2018/5/19. N2 - The release of ADP from platelet dense granules and its binding to platelet P2Y12 ...
Angiotensin II (Ang II) is a critical component of the reninangiotensin system that contributes to hypertension. Although platelets in blood from hypertensive subjects have an abnormal biological profile, it is unclear if circulating Ang II influences platelet aggregation or thrombus formation. One of the abnormalities presented to the platelets during hypertension is an elevated plasma concentration of serotonin (5-HT) caused by reduced 5-HT uptake secondary to loss of the 5-HT transporter (SERT) on the platelet plasma membrane. In the current study, we evaluated in vivo platelet function after 7 days of subcutaneous Ang II infusion to establish hypertension in mice and additionally assessed the biology of isolated platelets exposed to Ang II in vitro. The administration of Ang II elevated systolic blood pressure, but markers of platelet activation including P-selectin and PEJon/A staining were _disibledevent=font-size:10pt;line-height:1.5;font-family:Verdana;>5-HT in platelets, an event
Results: 22 hypoxemic patients were selected for the study based on their diagnosis of COPD (from history and spirometry) along with age and sex matched controls. Presence of comorbidities and other factors that cause platelet activation were excluded. Level of platelet aggregation was determined by several experiments. By an aggregometer using platelet agonists (thrombin and ADP) it was found that platelet aggregation was significantly higher in hypoxemic COPD patients than normal healthy controls. Fluorescence spectrophotometer was used to measure intracellular calcium as a marker of platelet activation and it was found that hypoxemic COPD patients had significantly higher platelet aggregation than normal healthy controls. However no significant difference was found in other markers of platelet activation studied namely P-selectin exposure and PAC-1 binding between the two groups ...
ABSTRACT. Angiotensin II (Ang II) is a critical component of the renin-angiotensin system that contributes to hypertension. Although platelets in blood from hypertensive subjects have an abnormal biological profile, it is unclear if circulating Ang II influences platelet aggregation or thrombus formation. One of the abnormalities presented to the platelets during hypertension is an elevated plasma concentration of serotonin (5-HT) caused by reduced 5-HT uptake secondary to loss of the 5-HT transporter (SERT) on the platelet plasma membrane. In the current study, we evaluated in vivo platelet function after 7 days of subcutaneous Ang II infusion to establish hypertension in mice and additionally assessed the biology of isolated platelets exposed to Ang II in vitro. The administration of Ang II elevated systolic blood pressure, but markers of platelet activation including P-selectin and PEJon/A staining were not changed. However, the aggregation response to collagen was reduced in isolated ...
Glycoprotein Ib-IX-V (GPIb-IX-V) is a platelet adhesion receptor complex that initiates platelet aggregation. Glycoprotein Iba (GPIba) is the central component of the GPIb-IX-V complex, anchoring the complex to the cytoskeleton and harboring the binding site for von Willebrand factor (vWF). Previous studies suggest that the coagulation function in pigs differs from that in humans, especially with respect to the interaction between vWF and platelets. However, we have little knowledge about the function of porcine platelets, which is important with regard to studies of cardiovascular disease, clotting, and surgery that use pigs as animal models. To extend this information, we cloned and analyzed the porcine GPIba sequence. Porcine GPIba contains 1891 nucleotides and includes an open reading frame that encodes 627 amino acids. The nucleotide sequence showed 67% identity with human GPIba, whereas the deduced amino acid sequences were 59% identical. The vWF binding domain shares the highest identity ...
BioAssay record AID 336312 submitted by ChEMBL: Antiplatelet activity against bovine citreated platelet assessed as inhibition of ADP-induced platelet aggregation up to 278 ug/ml after 6 mins.
TY - JOUR. T1 - Extensive characterization of the composition and functional activities of five preparations of human platelet lysates for dedicated clinical uses. AU - Delila, Liling. AU - Wu, Yu Wen. AU - Nebie, Ouada. AU - Widyaningrum, Rifa. AU - Chou, Ming Li. AU - Devos, David. AU - Burnouf, Thierry. N1 - Funding Information: LD and ON were supported by MS and PhD fellowships from Taipei Medical University, and RW by PhD fellowship from Ministry of Education of Taiwan. Publisher Copyright: © 2020 Taylor & Francis Group, LLC. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.. PY - 2021. Y1 - 2021. N2 - Human platelet lysates (HPLs), rich in various growth factors and cell growth-promoting molecules, encompass a new range of blood products that are being used for regenerative medicine, cell therapies, and tissue engineering. Well-characterized dedicated preparations, tailor-made to best fit specific therapeutic applications, are needed for optimal clinical efficacy and safety. ...
To delineate the critical top features of platelets necessary for balance and formation of thrombi, thromboelastography and platelet aggregation measurements were employed in whole blood of normal individuals and of those with Bernard-Soulier Syndrome (BSS) and Glanzmanns Thrombasthenia (GT). generation of stable thrombi, a potentially significant feature in individual medical results. Introduction An initial step in thrombus formation in the hurt vascular endothelium is the adhesion of platelets to shown subendothelial elements, e.g., von Willebrand Aspect (vWF), under high prices of shear, via the connections from the platelet glycoprotein (GP) 1b/V/IX receptor complicated with subendothelial vWF [1]. This tethering of platelets after that promotes their firmer binding to subendothelial collagen (COL) fibres via platelet receptors, e.g., GPVI [2], [3] and integrin II1 [4]. In this procedure, platelets are turned on, resulting in platelet shape adjustments, aggregation, discharge of aggregation ...
Octadecadienoic acids (linoleic acid and linolelaidic acid) and the diacylglycerol, 1-oleoyl-2-acetyl-rac-glycerol (OAG) concentration-dependently induced activation of gel-filtered human platelets, i.e. aggregation and phosphorylation of 20 kDa and 47 kDa peptides. In contrast, octadecenoic acids (oleic and elaidic acid) and octadecanoic (stearic) acid were inactive. Octadecadienoic acid-induced platelet activation was suppressed by the protein kinase C inhibitor, polymyxin B, but not by the cyclooxygenase inhibitor, indomethacin. OAG-induced activation was potentiated by octadecadienoic acids present at non-stimulatory concentrations. Our data suggest that octadecadienoic acids and diacylglycerol synergistically induce platelet activation via protein kinase C. Furthermore, linolelaidic acid may provide a useful experimental tool to study fatty acid regulation of protein kinase C in intact cells. ...
Staphylococcus aureus is an opportunistic pathogenic bacterium known for its ability to interact with platelets and modulate their function. S. aureus lipoproteins are one of the major groups of bacterial surface molecules and are released into the extracellular milieu where they are recognized by host immune cells. The aim of this study was to determine the role of S. aureus lipoproteins in S. aureus-platelet interactions. Platelet aggregation and binding assays using S. aureus wild type and lgt strains showed that, S. aureus lipoproteins contribute towards binding of the pathogen to platelets. Lipoproteins present in extracellular milieu also bind platelets. Platelet spreading, thrombus formation, agonist induced platelet aggregation and αIIbβ3 activation were inhibited by cell-free lipoproteins. CD36 was identified as the major platelet surface molecule interacting with S. aureus lipoproteins. Antibody neutralization demonstrated that functional inhibition of platelet activation caused by ...
Seventy-five consecutive patients with normal platelet counts were investigated for easy bruising. All had a normal coagulation profile, and all except four were women. None were on aspirin or other antiplatelet agents. Two specific groups could be delineated. In type I (44 patients, mean age, 35), platelet function was normal to supranormal. Megathrombocyte number was elevated in 60% of patients and correlated with the presence of antiplatelet antibody in 30% of patients. In type II (31 patients, mean age, 34), platelet function was abnormal: impaired epinephrine aggregation (primary and secondary wave) in 97%, impaired connective tissue aggregation in 77%, and impaired ADP aggregation in 42%. Megathrombocyte number was elevated in 71%, and antiplatelet antibody was present in 38% of patients. The easy bruising syndrome can be differentiated into two categories: type I, in which a platelet abnormality is unlikely, and type II, in which a platelet abnormality exists. Elevated incidence of ...
The work herein examines in vitro platelet aggregation in response to fluid shearing motion. Our specific aim is to characterize shear-induced aggregation by means of kinetic measurements. In doing so we consider plausible physicochemical mechanisms for platelet activation in the shear field. Besides resolving some questions concerning the activation of platelets by shear forces, this study further implicates fluid mechanical factors in thrombosis and arterial disease. Specific results may also apply to the design and evaluation of blood-contacting artificial devices. The experimental procedure centers on the use of a rotational viscometer to apply a controlled shearing motion to platelet suspensions for prescribed times. We quantify aggregation through changes in particle size histograms and associated measures (e.g. total number of particles). Additional insight into the aggregation response comes from interpreting kinetic data using the coalescence equation, a population balance specific for ...
It has been proposed that adsorbed glycoproteins such as fibrinogen and gamma-globulin induce platelet adhesion at blood-polymer interfaces. The importance of oligosaccharide groups in the glycoproteins proved to be responsible for platelet adhesion and aggregation via possible complex formation. Several studies have provided evidence that the proposed mechanism was involved in platelet adhesion on polymer surfaces. To minimize or prevent platelet adhesion on polymers, prostaglandins (PGs), potent inhibitors of platelet aggregation and PG-heparin (HEP) conjugate, were combined with polymers via physical dispersion or chemical immobilization on the surfaces. Albumin-HEP conjugate-adsorbed surfaces also showed significant reduction of platelet adhesion.
Human platelet-rich plasma (PRP) was irradiated in vitro with a fiberoptic Nd:YAG laser-heated metal cap to study its effects on platelets. The energy of the laser was 5 and 10 watts with an irradiation time of 0, 3, 6, and 9 seconds and 14 watts with an irradiation time of 0, 3, 4, and 5 seconds, respectively. The irradiated PRPs were analyzed for platelet count, aggregation reaction, thromboxane (TX)B2 measurement and electron microscopy. Various degrees of decrease in platelet count were observed in all groups. Except the 5Wx3S group, the other groups showed an increase in the maximum aggregation rate of platelets, which corresponded to the enhancement of TXB2 formation. It was also demonstrated by a transmission electron microscopy in 10Wx3S, 10Wx6S, 10Wx9S, 14Wx3S, 14Wx4S, and 14Wx5S energy groups that alpha- and dense-particles in irradiated platelets became sparse in number or even disappeared, less electron density, irregularity in size and shape, and a tendency for these particles to ...
This study was conducted to examine the mechanism(s) of synergistic interaction of adrenaline and platelet-activating factor (PAF) mediated human platelet aggregation. We found that platelet aggregation mediated by subthreshold concentrations of PAF (5-8 nM) plus adrenaline (0.5-2 μM) was inhibited by both α2-adrenoceptor blocker (yohimbine) and PAF receptor antagonist (WEB2086). While examining the role of the downstream signalling pathways, we found that this synergism was inhibited by calcium channel blockers, verapamil, and diltiazem. In addition, platelet aggregation by co-addition of adrenaline and PAF was also inhibited by very low concentrations of phospholipase C (PLC) inhibitor (U73122; IC50 = 0.2 μM), the MAP kinase inhibitor, PD98059 (IC50 = 3 μM) and cyclooxygenase (COX-1) inhibitors including indomethacin (IC50 = 0.25 μM), flurbiprofen (IC50 = 0.7 μM) and piroxicam (IC50 = 7 μM). However, the COX-2 inhibitor, nimesulide, was also effective in inhibiting the aggregation. The
The latter work was performed with murine species and, of note, many differences have been emphasized in megakaryocyte localization and ultrastructure of proplatelet formation, between mice and humans.32 The present study performed on human MKs sheds additional light on how these large protrusions can be induced to fragment into small platelets by shear forces in flowing blood. This is also in keeping with evidence that the pulmonary circulation could be an important site of platelet production, as the lung capillaries would be the first to be encountered by cells leaving the bone marrow.28 Indeed, the large cytoplasmic fragments and the isolated platelet-sized fragments that we observed in real time to form on the coverslip during the flow assay resembled those seen downstream of the pulmonary circulation in vivo.33 In our conditions, high shear rates were essential to proplatelet and platelet formation during an exposure time of 20 minutes. In contrast, no proplatelet or platelet was generated ...
REPORT DESCRIPTIONPlatelet rich plasma (PRP) is a blood plasma product in which concentration of platelets is elevated four to eight times higher than the normal blood platelet concentration. PRP is also called platelet rich gel, platelet enriched plasma and platelet enriched gels; and is used in effective treatment...
What Discussion about plateletsThrombocytopenia, platelet count, normal platelet count, high platelet count, low platelets, and other aspect
T cell depletion with antithymocyte globulins (ATG) can be complicated by thrombopenia and. hypercoagulability. The underlying mechanism is still unclear. We found that binding of ATG to. platelets caused platelet aggregation, alpha-granule release, membrane phosphatidylserine exposure. and the rapid release of platelet microvesicles (MV). Platelet activation and MV release. were complement-dependent and required membrane insertion of C5b-8 but not stable lytic pore. formation by C5b-9. Full platelet aggregation and activation by ATG also required the low affinity. Fc gamma receptor FcγRII. MV release, however, was FcγRII-independent. Platelet MV. expressed high prothrombinase activity. Moreover, blocking C5 inhibited ATG-induced thrombin. generation in platelet rich plasma. In 19 hematopoietic stem cell and kidney transplant patients,. ATG treatment resulted in thrombopenia and increased plasma levels of d-dimer and thrombin-anti-. thrombin-complexes. Flow cytometric analysis of complement ...
A platelet is a component of blood. Platelet or Platelets may also refer to: Platelet (horse), a racehorse Platelets (journal ... a scholarly journal Diamond platelet, a crystallographic defect of diamond Tectonic platelet, a minor tectonic plate This ... Look up platelet in Wiktionary, the free dictionary. ... page lists articles associated with the title Platelet. If an ...
It causes platelets to aggregate and blood vessels to dilate. Thus, it is important to the process of hemostasis. At a ... Investigation led to the understanding that platelet and blood pressure response were dependent on the sn-2 propionyl analog. ... Platelet-activating factor receptor Platelet-derived growth factor Zimmerman GA, McIntyre TM, Prescott SM, Stafforini DM (May ... which causes a drop in blood pressure and reduced volume of blood pumped by the heart, which leads to shock and possibly death ...
Naina HV, Harris S (2010). "Platelet and red blood cell indices in Harris platelet syndrome". Platelets. 21 (4): 303-6. doi: ... In the blood donors with HPS authors found a statistically higher MPV, RDW and a lower platelet count and platelet biomass. At ... platelets rarely < 50 × 109/L) with giant platelets (Mean platelet volume 10fL) and normal platelet aggregation studies with ... Harris platelet syndrome (HPS) is the most common inherited giant platelet disorder. HPS was identified among healthy blood ...
Blood. 136 (17): 1898-1900. doi:10.1182/blood.2020008196. ISSN 0006-4971. PMC 7582557. PMID 33091137. "Gray platelet syndrome ... The name derives from the initial observation of gray appearance of platelets with a paucity of granules on blood films from a ... GPS is characterized by "thrombocytopenia, and abnormally large agranular platelets in peripheral blood smears." The defect in ... "Orphanet: Gray platelet syndrome". Retrieved 2021-04-27. Gray platelet syndrome at NIH's Office of Rare Diseases ...
Autologous blood injection Autologous conditioned serum Hypoxia preconditioned plasma Platelet-rich fibrin matrix Platelet ... "Peripheral blood platelets express VEGF-C and VEGF which are released during platelet activation". Thrombosis and Haemostasis. ... is a concentrate of platelet-rich plasma protein derived from whole blood, centrifuged to remove red blood cells. Though ... Kolata G (2010-01-12). "Popular Blood Therapy May Not Work". New York Times. Reynolds G (2011-01-26). "Phys Ed: Does Platelet- ...
Platelets in blood collected into EDTA tubes appeared gray and agranular compared with platelets from blood in citrate or ... "Pseudo grey platelet syndrome--grey platelets due to degranulation in blood collected into EDTA". Eur. J. Haematol. 41 (4): 326 ... abnormally large agranular platelets in peripheral blood smears, and almost total absence of platelet alpha-granules and their ... Pseudo-gray platelet syndrome differs from gray platelet syndrome (GPS), one of the giant platelet syndromes. GPS is ...
Platelets can be produced either from whole blood donations or by apheresis. They keep for up to five to seven days. Platelet ... Platelets can be produced either from whole blood or by apheresis. They keep for up to five to seven days. Platelet ... "Patient Blood Management Guidelines , National Blood Authority". Archived from the original on 2016-01-15. ... Blood group matching (ABO, RhD) is typically recommended before platelets are given. Unmatched platelets, however, are often ...
When the blood vessel wall is damaged, platelet membrane glycoproteins interact with the extracellular matrix. Membrane ... Platelet membrane glycoproteins are surface glycoproteins found on platelets (thrombocytes) which play a key role in hemostasis ... Changes in the platelet membrane glycoprotein IIb.IIIa complex during platelet activation. The Journal of Biological Chemistry ... Activation of this complex initiates the platelet aggregation and the formation of primary platelet plug, a fibrin clot. The ...
2005). "WAVE/Scars in platelets". Blood. 105 (8): 3141-8. doi:10.1182/blood-2003-04-1319. PMID 15280206. S2CID 6674815. ... 2005). "Expression and subcellular localization of WAVE isoforms in the megakaryocyte/platelet lineage". J. Thromb. Haemost. 3 ...
Stoffel W, Heimann G, Hellenbroich B (1973). "Sphingosine kinase in blood platelets". Hoppe-Seyler's Z. Physiol. Chem. 354 (5 ...
Nugteren, D. H. (1975). "Arachidonate lipoxygenase in blood platelets". Biochimica et Biophysica Acta (BBA) - Lipids and Lipid ... Sub-primate mammals, such as the mouse, rat, rabbit, cow, and pig, express platelet type 12-lipoxygenase but also a leukocyte ... It was first found as a product of arachidonic acid metabolism made by human and bovine platelets through their 12S- ... Thus, the production of hepoxilins from 12(S)-HpETE may result from the intrinsic activity of platelet or leukocyte type 12- ...
Based predominantly on the presence of its mRNA, human ALOX12 is distributed predominantly in blood platelets and leukocytes ... Nugteren DH (February 1975). "Arachidonate lipoxygenase in blood platelets". Biochimica et Biophysica Acta (BBA) - Lipids and ... "Immunocytochemical localization of platelet-type arachidonate 12-lipoxygenase in mouse blood cells". The Journal of ... regulate regional blood flow, and contribute to the regulation of blood pressure in animal models (see Hepoxilins). Far more ...
When the lining of a blood vessel is broken, platelets are attracted, forming a platelet plug. These platelets have thrombin ... Blood platelets: biochemistry and physiology]". Hamostaseologie (in German). 23 (4): 149-58. doi:10.1055/s-0037-1619592. PMID ... the blockage of a vessel by an agglutination of red blood cells, platelets, polymerized fibrin and other components. ... Fibrin (also called Factor Ia) is a fibrous, non-globular protein involved in the clotting of blood. It is formed by the action ...
Wright, J. H. (1910). "The histogenesis of blood platelets". Journal of Morphology. 21 (2): 263-78. doi:10.1002/jmor.1050210204 ... Platelets are first named by James Homer Wright. Peyton Rous demonstrates that a malignant tumor can be transmitted by a virus ... Chicago cardiologist James B. Herrick makes the first published identification of sickle cells in the blood of a patient with ... Herrick, James B. (November 1910). "Peculiar elongated and sickle-shaped red blood corpuscles in a case of severe anemia". ...
... (PPP) is blood plasma with very low number of platelets (< 10 X 103/μL). Traditionally, PPP was ... "Platelet aggregation studies: autologous platelet-poor plasma inhibits platelet aggregation when added to platelet-rich plasma ... Man, D., Plosker, H., Winland-Brown, J.E. "The use of autologous platelet-rich plasma (platelet gel) and autologous platelet ... "Current concepts in Blood Management". Orthopaedics, 2004;27:S643-S641 R. Justin Thomas, Scott E. Marwin. "The role of fibrin ...
"Tachykinins regulate the function of platelets". Blood. 104 (4): 1058-65. doi:10.1182/blood-2003-11-3979. PMID 15130944. Page ...
First accurate counts of blood platelets. Traitement du choléra, G. Masson, Paris, 1885 - Treatment of cholera. Du sang et ses ... He performed the first accurate count of blood platelets, and is credited with developing a solution of mercury bichloride, ... Lecons cliniques sur les maladies du sang, G. Masson, Paris, 1900 - Clinical lessons on blood disorders. L'hématoblaste, ... sodium chloride and sodium sulfate for dilution of blood prior to counting erythrocytes with a hemocytometer. In 1874 he ...
Thrombocytopenia (low blood platelets) is unusual. The peripheral blood and bone marrow aspirate findings in copper deficiency ... Bone marrow aspirate in both conditions may show dysplasia of blood cell precursors and the presence of ring sideroblasts ( ... Symptoms may include fatigue, decreased red blood cells, early greying of the hair, and neurological problems presenting as ... Copper deficiency can have many hematological consequences, such as myelodysplasia, anemia, low white blood cell count, and low ...
... platelet life span, and platelet function in healthy human volunteers". Blood. 95 (8): 2514-22. doi:10.1182/blood.V95.8.2514. ... Platelets also secrete platelet-derived growth factor (PDGF). Platelets modulate neutrophils by forming platelet-leukocyte ... On a stained blood smear, platelets appear as dark purple spots, about 20% the diameter of red blood cells. The smear is used ... Pooled whole-blood platelets, sometimes called "random" platelets, are separated by one of two methods. In the US, a unit of ...
Giant platelets cannot stick adequately to an injured blood vessel walls, resulting in abnormal bleeding when injured. Giant ... Platelet Disorders Overview of Platelet Disorders at eMedicine Mhawech, Paulette (2000). "Inherited Giant Platelet Disorders". ... Abnormality of the abdomen, nosebleeds, heavy menstrual bleeding, purpura, too few platelets circulating in the blood, and ... This would utilize platelet aggregation studies and flow cytometry. Giant platelet disorders can be further categorized: caused ...
It is a turbid, light-yellow liquid that is obtained from human blood platelets after freeze/thaw cycle(s). The freeze/thaw ... hPL is created from single or pooled donor-donated platelets isolated from whole blood or by apheresis, distributed in a ... Platelet lysate offers a true human-based recombinant protein platform. Platelet lysate has been tested in various cell culture ... Human Platelet Lysate, XcytePlus, PLSOLUTION, PLMATRIX and CRUX RUFA Media Supplements. Platelet lysate has also been produced ...
Megakaryocytes release platelets into the bloodstream. Platelets are critical for normal blood clotting. In consequence of this ... Blood samples are obtained from the fetal umbilical cord to determine blood cell counts, measure blood enzymes to evaluate ... decreased numbers of circulating platelets and red blood cells, and increased numbers of circulating white blood cells. Also ... reduced platelet production often accompanied by significantly reduced levels of circulating platelets; reduced red blood cell ...
Preservation of Blood Platelets. US Patent Number 5,876,676, issued March 2, 1999. 7. Stossel, TP, Hartwig, JH, Hoffmeister, KM ... Effect of corticosteroid therapy on the phagocytosis of antibody-coated platelets by human leukocytes. Blood. 1978; 51:771-779 ... The clearance mechanism of chilled blood platelets. 2003. Cell, 112: 87-97. 126. Hoffmeister, KM, Josefsson, EC, Isaac, NA, ... Glycosylation restores survival of chilled blood platelets. 2003. Science 301: 1532-1534. 127. Woo, MS, Ohta, Y, Rabinovitz, I ...
Fresh venous clots are red blood cell and fibrin rich. Platelets and white blood cells are also components. Platelets are not ... Individuals without O blood type have higher blood levels of von Willebrand factor and factor VIII than those with O blood type ... NETs provide "a scaffold for adhesion" of platelets, red blood cells, and multiple factors that potentiate platelet activation ... Blood has a natural tendency to clot when blood vessels are damaged (hemostasis) to minimize blood loss. Clotting is activated ...
1990). "Gova/b alloantigen system on human platelets". Blood. 75 (11): 2172-6. doi:10.1182/blood.V75.11.2172.2172. PMID 2346781 ... 1991). "Identification of a cell-surface antigen associated with activated T lymphoblasts and activated platelets". Blood. 77 ( ... 2002). "A tyrosine703serine polymorphism of CD109 defines the Gov platelet alloantigens". Blood. 99 (5): 1692-8. doi:10.1182/ ... and activated platelets (Lin et al., 2002). In addition, the platelet-specific Gov antigen system (HPA15), implicated in ...
Her blood vessels were largely filled with platelets. Modern reports still occasionally refer to TTP as "Moschcowitz disease" ...
MAO-B is mostly found in blood platelets. MAO-A appears at roughly 80% of adulthood levels at birth, increasing very slightly ... Domino EF, Khanna SS (March 1976). "Decreased blood platelet MAO activity in unmedicated chronic schizophrenic patients". The ... Schildkraut JJ, Herzog JM, Orsulak PJ, Edelman SE, Shein HM, Frazier SH (April 1976). "Reduced platelet monoamine oxidase ... Oreland L (January 2004). "Platelet monoamine oxidase, personality and alcoholism: the rise, fall and resurrection". ...
White blood cells help to resist infections and parasites. Platelets are important in the clotting of blood. Arthropods, using ... mixed-blood and blood relative. Autotransfusion Blood as food Blood pressure Blood substitutes ("artificial blood") Blood test ... and the blood cells it carries, peripheral blood cells. Blood is composed of blood cells suspended in blood plasma. Plasma, ... of blood is blood plasma, a fluid that is the blood's liquid medium, which by itself is straw-yellow in color. The blood plasma ...
Thaulow, E; Erikssen, J; Sandvik, L; Stormorken, H; Cohn, PF (August 1991). "Blood platelet count and function are related to ... Holmsen, H; Day, HJ; Stormorken, H (1969). "The blood platelet release reaction". Scandinavian Journal of Haematology. ... Gogstad, GO; Stormorken, H; Solum, NO (15 July 1983). "Platelet alpha 2-antiplasmin is located in the platelet alpha-granules ... Day, HJ; Stormorken, H; Holmsen, H (1973). "Subcellular localization of platelet factor 3 and platelet factor 4". Scandinavian ...
Thrombocytopenia is a deficiency of platelets in the blood. It can present as red blood blisters in the mouth. Patients with ... A heart attack is a blood vessel in the heart being constricted either by a blood clot or atherosclerosis formation. A heart ... High blood sugar can be detected by sampling saliva. Saliva sampling may be a non-invasive way to detect changes in the gut ... Sickle cell disease is a hereditary genetic condition that results in deformed red blood cells to be formed. Sickle patients ...
... particularly prostacyclin which is found in blood vessel lining. Prostacyclin relaxes or unsticks platelets, so selective COX-2 ... It sticks platelets together and promotes clotting; inhibiting this helps prevent heart disease. On the other hand, PTGS2 (COX- ...
Most studies have looked at the receptor tyrosine kinases and examples of these are platelet derived growth factor receptor ( ... Blood Cancer J. 3 (11): 61. doi:10.1038/s41408-021-00450-2. PMC 7973815. PMID 33737511. src+Gene at the US National Library of ...
Platelets are cellular fragments formed from protrusions on megakaryocytes that enable blood clotting. Blood symptoms have not ... One affected person was reported to have a reduced number of platelets (thrombocytopaenia) in infancy, requiring transfusion. ... and less commonly there may be twisted retinal blood vessels or optic nerve hypoplasia. The eye anomalies can result in an ...
... blood platelets) and thereby the development, maintenance, and/or worsening of the cited autoimmune diseases. Further studies ... On the other hand, the CpG cluster(s) controlling the CMTM5 gene in the blood of individuals with the autoimmune disease of ... A case-control study of hospitalized patients found that the blood plasma levels of CMTM5 protein and CMTM5 messenger RNA (i.e ... Studies find that the CMTM5 gene in the DNA isolated from the blood of individuals with the autoimmune diseases of systemic ...
... (EC, Russell's viper venom factor X activator, RVV-X, blood-coagulation factor X activating enzyme, ... platelet aggregation inhibitor)-like and C-type lectin-like domains". The Journal of Biological Chemistry. 267 (20): 14109-17. ... This enzyme catalyses the following chemical reaction Specifically activates several components of the blood clotting system, ... metalloproteinase RVV-x, Vipera russelli proteinase, Russell's viper blood coagulation factor X activator, RVV-V) is an enzyme ...
The most common side effects include changes in laboratory tests (including increased liver enzymes, increased blood sugar, ... decreased white cell and platelet counts, decreased protein level, decreased calcium, increased total cholesterol, increased ... Selpercatinib can cause serious side effects including liver toxicity, high blood pressure, heart rhythm changes due to ... creatinine, and decreased sodium) dry mouth, diarrhea, high blood pressure, fatigue, edema, rash, and constipation. ...
Abnormal laboratory findings seen in patients with Rocky Mountain spotted fever may include a low platelet count, low blood ... resulting in mononuclear cell infiltration into blood vessels and subsequent red blood cell leakage into surrounding tissues. ... Through a series of discoveries, the team found that a previous blood meal was necessary to make the tick deadly to its hosts, ... Ticks can also become infected with R. rickettsii while feeding on blood from the host in either the larval or nymphal stage. ...
... low levels of platelets in the body-an important component of blood clotting-deficiency results in worse bleeding). Ticks can ... Soft ticks remain attached on the order of a couple hours and may take multiple blood meals from the same host. Hard ticks on ... After attachment, ticks gain access to a hosts blood supply via use of sharp projections from their mouth known as chelicerae. ... Ticks are insects known for attaching to and sucking blood from land-dwelling animals (specifically vertebrates). Ticks fall ...
With reported unusual blood cloths with persons having low platelet count after inoculation, DOH temporarily stopped the use of ...
The common myeloid progenitor can differentiate in the bone marrow into red blood cells and megakaryocytes (leading to ... platelets) as well as mast cells and myeloblasts, the latter leading to the myelocytic line (granulocytes) and to monocytes, ... all blood cells. In a narrower sense, myelopoiesis also refers specifically to the regulated formation of myeloid leukocytes ( ...
... the presence of high platelet (thrombocyte) counts in the blood This disambiguation page lists articles associated with the ...
... red blood cells, white blood cells and platelets. Over 150 liters of fluid enter the glomeruli of an adult every day: 99% of ... The glomerular blood pressure provides the driving force for water and solutes to be filtered out of the blood plasma, and into ... Normally the only components of the blood that are not filtered into Bowman's capsule are blood proteins, ... Distribution of blood vessels in cortex of kidney. (Although the figure labels the efferent vessel as a vein, it is actually an ...
... the growth of blood vessels in tumors. He and his team discovered that inhibiting a key player in the signaling system (called ... These include all receptors for platelet-derived growth factor (PDGFR) and vascular endothelial growth factor receptors (VEGFR ... vascular endothelial growth factor receptor or VEGFR) suppresses the generation of blood vessels in tumors and slows down ...
Thromboxane A synthase 1 (EC, platelet, cytochrome P450, family 5, subfamily A), also known as TBXAS1, is a cytochrome ... This control becomes an important factor in several processes, such as blood pressure regulation, clotting, and inflammatory ... This enzyme, anchored to the endoplasmic reticulum, is found in platelets, monocytes, and several other cell types. The NH2 ... "Entrez Gene: TBXAS1 thromboxane A synthase 1 (platelet, cytochrome P450, family 5, subfamily A)". Yokoyama C, Miyata A, Ihara H ...
Doctors diagnosed a low blood platelet count and Dolan began a series of blood transfusions, after each, he felt better for a ... Despite the blood transfusions and other medical interventions, Dolan became weaker and he was finally discharged from the ...
Blood. 65 (4): 951-958. doi:10.1182/blood.v65.4.951.951. PMID 3872142. Begley, C. Glenn; Lopez, A. F; Nicola, N. A; Warren, D. ... This is a hormone that regulates platelet production. He also had his most cited paper in 2007 titled "Genome-wide association ... Blood. 68 (1): 46-57. doi:10.1182/blood.v68.1.46.46. PMID 3087441. Sheridan, W. P; Fox, R. M; Begley, C. Glenn; Maher, D; ... McGrath, K. M; Mostyn, G (1992). "Effect of peripheral-blood progenitor cells mobilised by filgrastim (G-CSF) on platelet ...
... or who have abnormally low levels of white or red cells or platelets in the blood, should be investigated for possible ... history of tick exposure and possibly testing for specific antibodies in the blood. Blood tests are often negative in the early ... If the removed tick is full of blood a single dose of doxycycline may be used to prevent the development of infection but is ... Unlike blood and intrathecal antibody tests, CSF pleocytosis tests revert to normal after infection ends and therefore can be ...
Raph blood group system in the BGMUT blood group antigen gene mutation database Human CD151 genome location and CD151 gene ... Fitter S, Tetaz TJ, Berndt MC, Ashman LK (1995). "Molecular cloning of cDNA encoding a novel platelet-endothelial cell tetra- ... span antigen, PETA-3". Blood. 86 (4): 1348-55. doi:10.1182/blood.V86.4.1348.bloodjournal8641348. PMID 7632941. Maruyama K, ... CD151 molecule (Raph blood group), also known as CD151 (Cluster of Differentiation 151), is a human gene. The protein encoded ...
August 2005). "Impact of the CYP2D6 ultra-rapid metabolizer genotype on doxepin pharmacokinetics and serotonin in platelets". ... rarely high blood pressure. May increase or decrease liver function in some people. The side effects of low-dose doxepin for ... Low blood pressure, (if patient arises too fast from the lying/sitting position to standing-known as orthostatic hypotension), ... introducing blood level monitoring and genotype testing". Journal of Dermatological Treatment. 33 (1): 1-7. doi:10.1080/ ...
... with red blood cells used for anemic individuals, platelets for cancer patients, and plasma for those with decreased blood ... platelets, white blood cells, and red blood cells, greatly increasing the efficiency of the entire transfusion process; now ... Klein developed a technique that allowed the separation of whole human blood into its component parts of plasma, ...
... such as red blood cells, white blood cells and platelets. The use of blood stem cells has emerged as a potentially curative ... As a public cord blood bank, SCBB does not charge cord blood donors for its services. However, as the cord blood is donated ... but retain ownership over the use of their cord blood. Cord blood is the blood that circulates through the umbilical cord from ... because cord blood is rich in blood stem cells. Blood stem cells are young or immature cells that can transform into other ...
Within blood, thrombins cleave fibrinogens to fibrins during coagulation and a fibrin-based blood clot forms. Factor XIII is a ... A subunits of human factor XIII are made primarily by platelets and other cells of bone marrow origin. B subunits are secreted ... A and B units combine within blood to form heterotetramers of two A units and two B units. Blood plasma concentration of the ... Factor XIII or fibrin stabilizing factor is a zymogen found in blood of humans and some other animals. It is activated by ...
Misra S, Kumar A, Kumar P, Yadav AK, Mohania D, Pandit AK, Prasad K, Vibha D (September 2017). "Blood-based protein biomarkers ... Davies RS, Abdelhamid M, Wall ML, Vohra RK, Bradbury AW, Adam DJ (September 2011). "Coagulation, fibrinolysis, and platelet ... "The APC-PCI concentration as an early marker of activation of blood coagulation: a study of women on combined oral ...
Other laboratory findings in Lassa fever include lymphocytopenia (low white blood cell count), thrombocytopenia (low platelets ... Fluid replacement, blood transfusions, and medication for low blood pressure may be required. Intravenous interferon therapy ... or dangerously low blood pressure may occur. Long term complications may include hearing loss. In those who are pregnant, ... to avoid contact with blood and body fluids. These issues in many countries are monitored by a department of public health. In ...
Blood. 84 (1): 184-8. doi:10.1182/blood.V84.1.184.184. PMID 8018916. Le Beau MM, Espinosa R, Neuman WL, Stock W, Roulston D, ... It also has many more specific effects like the regeneration of platelets and potentially aids in early antibody isotype ... Blood. 87 (1): 30-7. doi:10.1182/blood.V87.1.30.30. PMID 8547656. Hirst, WJR; Buggins, A; Darling, D; Gäken, J; Farzaneh, F; ... doi:10.1182/blood.V68.1.46.46. PMID 3087441. Serrano F, Varas F, Bernard A, Bueren JA (1994). "Accelerated and longterm ...
A low white blood cell count, and low platelet count in the blood may be observed. A low level of neutrophils (a specific type ... Blood tests show the level of IgM in the blood and the presence of proteins, or tumor markers, that are the key signs of ... Treon, S. P. (2009). "How I treat Waldenström macroglobulinemia". Blood. 114 (12): 2375-2385. doi:10.1182/blood-2009-05-174359 ... Blood. 110 (13): 4417-4426. doi:10.1182/blood-2007-05-092098. PMC 2234792. PMID 17761832. Mensah-Osman, E.; Al-Katib, A.; ...
... an image generator for serious simulation Mean platelet volume, in blood testing, a measure of platelet size Meerwein-Ponndorf- ...
In circulating platelets there is no neutral SMase activity, but they do have S-SMase enzymatic activity. It has been shown ... Diagnosis is confirmed by an aSMase activity less than 10% in the peripheral blood lymphocytes. Caused by a mutation in the ... that in response to thrombin induced platelet activation, S-SMase is released extracellulary and a parallel decrease in ...
... machinery is used for intraoperative blood salvage. A centrifuge process takes blood from the patient, washes the red blood ... This reduces NO-dependent vasodilation and induces platelet activation, thrombin generation, procoagulant factors and tissue ... Hemolysis may result from intrinsic defects in the red blood cell itself: Defects of red blood cell membrane production (as in ... red blood cell precursors) to compensate for the loss of red blood cells due to hemolysis. Hemolysin Glucose-6-phosphate ...
Blood. 95 (8): 2505-13. doi:10.1182/blood.V95.8.2505. PMID 10753828. Gesbert F, Garbay C, Bertoglio J (1998). "Interleukin-2 ... "Identification of Tyr-762 in the platelet-derived growth factor alpha-receptor as the binding site for Crk proteins". Oncogene ... "Differential interaction of CrkII adaptor protein with platelet-derived growth factor alpha- and beta-receptors is determined ...
Clinicians need to be aware of bacterial contamination of blood products, especially platelets. ... In practice, the type of platelet donation (apheresis or whole blood-derived platelets) has dictated the bacterial detection ... Clinician is contacted by the blood collection or transfusion service with information involving a blood or blood component ... Most blood collection centers culture apheresis platelets (derived from single donors) and release the unit after the culture ...
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Platelets are a part of the blood that helps the blood clot. ... shows if you have antibodies against platelets in your blood. ... This blood test shows if you have antibodies against platelets in your blood. Platelets are a part of the blood that helps the ... Abnormal results show that you have anti-platelet antibodies. Anti-platelet antibodies may appear in the blood due to any of ... Platelet and blood vessel disorders. In: Kliegman RM, St. Geme JW, Blum NJ, Shah SS, Tasker RC, Wilson KM, eds. Nelson Textbook ...
Safe blood and blood products : manual on the management, maintenance and use of blood cold chain equipment  ... The blood cold chain : guide to the selection and procurement of equipment and accessories  ... Collaborative study to enlarge the first WHO repository of platelet transfusion-relevant bacterial reference strains: Expert ...
Platelets are a part of the blood that helps the blood clot. Learn more here. ... This blood test shows if you have antibodies against platelets in your blood. ... This blood test shows if you have antibodies. against platelets in your blood. Platelets are a part of the blood that helps the ... Abnormal results show that you have anti-platelet antibodies. Anti-platelet antibodies may appear in the blood due to any of ...
Here, we unveil and quantify the structural mechanisms of clot contraction at the level of single platelets. A key elementary ... Blood clot contraction plays an important role in prevention of bleeding and in thrombotic disorders. ... step of contraction is sequential extension-retraction of platelet filopodia attached to fibrin fibers. In contrast to other ... The platelet integrin αIIbβ3 forms a transmembrane link between fibrin outside the platelet and actin inside the platelet6, 7 ...
In a healthy person, a large number of platelets are manufactured and stored in the body. ... Platelets are the component of blood that helps with clotting. ... Blood * * Am I eligible to donate blood? * ABCs of eligibility ... Platelets are the component of blood that helps with clotting. If an injury or blood loss occurs, platelets are released, and a ... How platelets work. Platelets are about a quarter of the size of red blood cells and are not whole cells but rather fragile ...
Drugs with effects on vascular endothelium, platelets, red blood cells. Class Summary. These drugs appear to have multiple ... The effect of aspirin on platelet function can be assessed by optical platelet aggregometry or Platelet Function Analyzer (PFA- ... Platelet count enhancers. Class Summary. These agents are used to augment platelet recovery. ... Platelet adhesion inhibitor that possibly inhibits RBC uptake of adenosine, which is an inhibitor of platelet reactivity. In ...
Platelets are critically needed every day for some of the ... fragments of blood cells that are essential for normal blood ... Platelets. Platelets are tiny disk-shaped fragments of blood cells that are essential for normal blood clotting. Platelets are ... Blood Types. Each person has a specific blood type, based on the combination of factors included in their blood. This blood ... Blood. Whole blood donations are the most common form of donation. In it, you will donate a pint of blood in a process that ...
Synthetic platelets are in high demand in the medical field due to their ability to bypass the immune ... the building blocks of blood that assist with clotting - with the help of collaborators at Sanford-Burnham Institute and ... UCSB researchers have discovered a new and more effective method of constructing synthetic platelets - ... The research, titled "Platelet Mimetic Particles for Targeting Thrombi in Flowing Blood," was published in Tuesdays edition of ...
Donate blood today to help those in need. ... Blood donors report feeling a sense of great satisfaction after ... making their blood donation. Why? Because helping others in need just feels good. ... Blood donors who are Black play a critical role in helping sickle cell disease patients receive the most compatible blood match ... Am I Eligible to Donate Blood?. Are you eligible for blood donation? Find out about the eligibility requirements to donate ...
... namely red blood cells, white blood cells and platelets, also known thrombocytes. A lack of platelets in your body may cause ... A low platelet count may also be known as thrombocytopenia ... Blood is made up of various types of cells, ... 7 Foods To Increase Blood Platelets. Blood is made up of various types of cells, namely red blood cells, white blood cells and ... blood clots cannot form. Blood is made up of various types of cells, namely red blood cells, white blood cells and platelets, ...
Blood Platelet Stress Reliever on sale for $1.60. Measures 3.75"x3.75"x3.75" - 0.08 lbs and imprint area of 1& ... Blood Platelet Stress Relievers. Personalized stress balls min. order of 150 pieces. Red and Blue colors in stock! Imprint area ...
Community Blood Drives allow the UCLA Blood and Platelet Center to interact with community members while providing donors with ... The goal of the UCLA Blood & Platelet Center is to provide patients with safe blood collected from volunteer donors. Like all ... Welcome to the Community Blood Drives. In partnership with many community groups, the UCLA Blood and Platelet Center travels to ... All blood drives follow a similar process, however, they are not all the same. Some blood drives are large others are small. ...
The platelet aggregation of each test drug was measured by the screen filtration pressure method using whole blood of guinea ... To ensure the specificity of KBG, tokishakuyakusan (TSS) and kamisyoyosan (KSS), which are known to have platelet aggregation- ... Furthermore, paeonol, a representative component of Moutan cortex, and aspirin which is known to have platelet aggregation- ... These results suggest that the platelet aggregation-inhibiting activity of the constituent crude drugs Moutan cortex and ...
Earlier transfusion with higher blood product ratios (plasma, platelets, and red blood cells), defined as damage control ... Transfusion of plasma, platelets, and red blood cells in a 1:1:1 vs a 1:1:2 ratio and mortality in patients with severe trauma ... and platelets (12 U vs 6 U, P , .001) and similar amounts of red blood cells (9 U) over the first 24 hours, no differences ... platelets, and red blood cells in a 1:1:1 ratio compared with a 1:1:2 ratio. ...
Tags: blood donations, cancer patients, platelets, Rhode Island Blood Center, trauma patients ... LeBlanc says, "We are suffering from a significant shortage of platelets.". Platelets help produce blood clots to control ... Platelet donors can give more frequently compared to whole blood or red cell donors, as often as every 2 weeks, up to 24 times ... Rhode Island Blood Center needs platelet donors to replenish low supplies. Cancer patients, trauma patients, and people with ...
Platelets Donation. Our platelet donation clinics are in Dublin and Cork. Platelets are used in the treatment of cancer and ... Learn About Blood. Blood group basics, interesting facts, testing, what blood is used for ... The Irish Blood Transfusion Service provides life-saving platelets to all of the hospitals in Ireland. Due to the rising number ... You may however be eligible to become a blood donor if you received blood in Republic of Ireland before 1980; please check the ...
Platelets Donation. Our platelet donation clinics are in Dublin and Cork. Platelets are used in the treatment of cancer and ... Learn About Blood. Blood group basics, interesting facts, testing, what blood is used for ... However, platelets have the shortest shelf life of all blood components, lasting only 5 to 7 days. This means we have a ... We need every blood group every single day. Many of Irelands patients rely on platelets, so it is important supplies are ...
Despite the widespread reliance on serum and plasma as analytes, we found that the platelet fraction of blood concentrates ... The potential for platelets to serve as a reservoir for Borrelia and its diagnostic targets may transform direct clinical ... The low bacterial burden in vasculature and lack of consensus around blood-based isolation of the causative pathogen, Borrelia ... We therefore addressed methodological optimization of Borrelia recovery from blood, first by analyzing existing protocols, and ...
Blood platelet RNA-sequencing is increasingly used among the scientific community. Aberrant platelet transcriptome is common in ... Transcriptomic landscape of blood platelets in healthy donors. Supernat, Anna; Popęda, Marta; Pastuszak, Krzysztof; Best, Myron ... Our work provides a reference for studies working both on healthy platelets and pathological conditions affecting platelet ... Healthy donor platelet transcriptome retains general consistency, with very few splice variants deviating from the landscape. ...
Washed platelets loaded with [3H]-5-hydroxytryptamine (5-HT) were incubated with monoamines; the amount of radioactivity ... of digital blood vessels obtained from 6 healthy mixed-breed horses and ponies euthanatized at an abattoir and platelets ... All amines tested released 5-HT from platelets. Amines formed in the cecum and released into the systemic circulation warrant ... All 5 amines displaced 5-HT from platelets with the order of potency being TYR , TRP , PEA , IAA , IBA. Conclusions and ...
... the American Red Cross is urging Oregonians to donate blood, in hopes of avoiding a shortage as the holiday season nears. ... Those who donate blood or platelets from Nov. 1 to 22 will receive a $10 e-gift card to a chosen merchant. Donations made over ... The organization is looking for both blood and platelet donations. The Red Cross said its worried that many donors will come ... Red Cross asks for blood, platelet donations ahead of holidays by: Hailey Dunn ...
This test is indicated for assessing platelet ultra-structural abnormalities in congenital and acquired platelet disorders.. ... This test is indicated for assessing platelet ultra-structural abnormalities in congenital and acquired platelet disorders.. ... 1. Platelet Esoteric Testing Patient Information is required. See Special Instructions.. 2. If not ordering electronically, ... The dense bodies of human platelets: inherent electron opacity of the serotonin storage particles. Blood. 1969 Apr;33[4]:598- ...
A trial assessing clinically relevant outcomes in relation to the platelet count thresholds commonly used as triggers for ... Approximately 25% of these babies have a low platelet count and require platelet transfusions. However, views amongst those ... The differences between these groups were the platelet count at which they received a platelet transfusion. In one arm babies ... The trial was funded and sponsored by NHS Blood and Transplant (NHSBT) and run through the NHSBT Clinical Trials Unit (CTU). ...
adhesion; aspirin; blood flow; blood platelets; blood sampling; cardiovascular diseases; cell adhesion; death; diagnostic ... blood platelets Remove constraint Subject term: blood platelets Text Availability Citation in PubAg Remove constraint Text ... aspirin; blood platelets; blood serum; cardiovascular diseases; chemokine CXCL4; hypercholesterolemia; ligands; light ... adenosine diphosphate; adenosine triphosphate; aspirin; blood platelets; blood serum; cAMP-dependent protein kinase; ...
Oxidized LDL activates blood platelets through CD36/NOX2-mediated inhibition of the cGMP/protein kinase G signaling cascade ... 9 more authors) (2017) Oxidized LDL activates blood platelets through CD36/NOX2-mediated inhibition of the cGMP/protein kinase ... Platelets from hyperlipidemic mice were also found to have a diminished sensitivity to cGMP when tested ex vivo, a phenotype ... This was prevented by either pharmacologic inhibition of NOX2 in human platelets or genetic ablation of NOX2 in murine ...
  • Bacterial contamination of platelet components is the second most common cause of transfusion-related deaths in the United States. (
  • To address this risk, the Association for the Advancement of Blood & Biotherapies (AABB) adopted a standard that requires blood collection and transfusion service members to detect and limit bacterial contamination in all platelet components. (
  • Bacterial contamination of a blood component is not typically considered in the differential diagnosis of a transfusion-related illness because signs and symptoms (including fever, rigors, and change in blood pressure) resemble those expected from either a transfusion reaction or sepsis due to any cause. (
  • For the past several years, bacterial contamination of platelets has been the greatest transfusion-transmitted infectious risk in the United States. (
  • The actual risk of transfusion-associated sepsis is likely higher, as infections due to contaminated blood products are under-reported. (
  • It is critical for clinicians to be aware of the problem of bacterial contamination of blood products, particularly platelets, and to consider the possibility of bacterial contamination when investigating febrile transfusion reactions. (
  • Clinicians should collaborate with hospital transfusion services and blood collection center personnel, and, when necessary, notify health departments, to manage suspected infections in blood donors and transfusion recipients. (
  • If bacterial contamination of a component is suspected, the transfusion should be stopped immediately, the unit should be saved for further testing, and blood cultures should be obtained from the patient receiving the transfusion. (
  • Clinician is contacted by the blood collection or transfusion service with information involving a blood or blood component with bacterial contamination. (
  • The Irish Blood Transfusion Service provides life-saving platelets to all of the hospitals in Ireland. (
  • Have you ever had a blood transfusion? (
  • Thank you for your interest in in becoming a platelet donor, unfortunately you are not eligible if you have ever received a transfusion of a blood product of any kind. (
  • PlaNeT-2/MATISSE is a trial assessing clinically relevant outcomes in relation to the platelet count thresholds commonly used as triggers for transfusion. (
  • However, views amongst those working in neonatal medicine differ as to the safest platelet level to use as a trigger for transfusion. (
  • The differences between these groups were the platelet count at which they received a platelet transfusion. (
  • In one arm babies were transfused when the platelet count dropped below 50 x109/L. In the other arm, the level had to drop to below 25 x109/L before a transfusion was triggered. (
  • When Is A Platelet Transfusion Needed? (
  • Transfusion is necessary when a patient with Dengue Haemorrhagic Fever (DHF) shows a severe drop in platelet count. (
  • Every 2 seconds, someone needs a blood transfusion. (
  • Categorizing blood according to type helps prevent reactions when someone gets a blood transfusion. (
  • To get a blood transfusion safely, a person's immune system must recognize the donor cells as a match to his or her own cells. (
  • This means that you can only get a transfusion from someone with A or O blood, not from someone with B or AB blood. (
  • So as a person with type B blood, you could get a transfusion from someone with B or O blood, but not A or AB. (
  • A person with O blood can only get a transfusion with O blood. (
  • A platelet count below this level does not in itself indicate a need for platelet transfusion. (
  • Recent studies indicate that the clinically stable patient is unlikely to benefit from prophylactic platelet transfusion if the count is greater than 10 x 109/l. (
  • However, some experts question the usefulness of the platelet count in the peripheral blood as a guide to the risk of bleeding or as a means for assessing the effect of platelet transfusion. (
  • Clinical transfusion guidelines for platelet transfusion usually cover the management of bleeding during surgery or patients with bone marrow suppression and the prevention of bleeding in patients with low platelet count due to bone marrow suppression or other causes (PMID 19109560 , 15495093 , 15584985 , 16351634 ). (
  • He underwent an immediate splenectomy with massive transfusion: 9 packed red blood cells units, 7 fresh frozen plasma units, and 1 whole blood platelet pool. (
  • White cells and platelets in blood transfusion : proceedings of the Eleventh Annual Symposium on Blood Transfusion, Groningen 1986, organized by the Red Cross Blood Bank Groningen-Drenthe / edited by C. Th. (
  • The Star]Most blood donors and recipients may not know that the whole blood they donate is not directly used for transfusion as collected. (
  • Did she/he EVER receive a blood transfusion or other medical treatment outside of the United States or Canada? (
  • Every two seconds, a patient somewhere in the U.S. needs a blood transfusion. (
  • BACKGROUND: The AABB (formerly, the American Association of Blood Banks) developed this guideline on appropriate use of platelet transfusion in adult patients. (
  • Thrombocytopenia caused by platelet destruction, hypersplenism, or hemodilution. (
  • Any count is higher than this normal platelet count range, known as high platelet count, resulting in thrombocytopenia. (
  • Thrombocytopenia is an autoimmune bleeding disorder in which the number of platelets in the blood is significantly lower than usual. (
  • We also discuss what makes platelets change shape and the symptoms, diagnosis, and treatment of thrombocytopenia. (
  • The lower level of the platelet is known as thrombocytopenia . (
  • Wiskott-Aldrich syndrome (see the image below) is an X-linked recessive immunodeficiency disorder characterized in one third of patients by the triad of recurrent bacterial sinopulmonary infections, eczema (atopiclike dermatitis), and a bleeding diathesis caused by thrombocytopenia and platelet dysfunction. (
  • His diagnosis was confirmed by immunologic parameters, thrombocytopenia, and low platelet volume. (
  • And thrombocytopenia is a condition in which you have low blood platelet count, defined as less than 150. (
  • So, the discussion around this issue or awareness of this issue originated from reports of a rare but serious condition following AstraZeneca's COVID-19 vaccine, and this condition initially recognized was CVST in the presence of thrombocytopenia, so, blood clots in the brain with low platelets. (
  • This can help in excluding platelet clumps as a cause of apparent thrombocytopenia and in identifying the presence of abnormal-looking WBCs or giant platelets. (
  • Low levels of platelets is called thrombocytopenia . (
  • A single platelet treatment would require six to eight whole blood donors instead of just one plateletpheresis donor. (
  • Regular donors can give platelets every two weeks. (
  • New platelet donors are encouraged to donate once a month. (
  • The short shelf life of platelets - just 5 days after they're collected, tested and processed - also means we need donors every day of the year, including holidays. (
  • Platelet donors must weigh at least 110 pounds. (
  • Platelets are most often given at donor centers, where we can make donors comfortable with reclining chairs and individual televisions. (
  • Because only platelets are removed, donors can give more often - as often as every 7 days. (
  • Blood donors who are Black play a critical role in helping sickle cell disease patients receive the most compatible blood match. (
  • Community Blood Drives allow the UCLA Blood and Platelet Center to interact with community members while providing donors with a rewarding donation experience. (
  • In addition, blood drives provide UCLA patients with an adequate and safe supply of blood coming from donors within our communities. (
  • In partnership with Be The Match®, operated by the National Marrow Donor Program®, donors have the opportunity to register at a majority of our blood drives to be part of the largest and most diverse marrow registry in the world. (
  • The goal of the UCLA Blood & Platelet Center is to provide patients with safe blood collected from volunteer donors. (
  • PROVIDENCE, R.I. (WLNE) - The Rhode Island Blood Center is asking donors to step up with a very specific request. (
  • Platelet donors can give more frequently compared to whole blood or red cell donors, as often as every 2 weeks, up to 24 times a year. (
  • Due to the rising number of cancer diagnosis in the country, there is always a need for platelet donors. (
  • There are just 2,400 Irish platelet donors and we are looking for new donors to join the panel. (
  • This means we have a constant need for platelet donors to ensure we meet the needs of these patients. (
  • Datasets from 204 healthy donors were used for the analysis of splice variants, particularly with regard to age, sex, blood storage time, unit of collection or library size. (
  • However, when donors are analysed globally (as vectors), sex, storage time, library size, the unit of blood collection as well as age impose a certain degree of between- and/or within-group variability. (
  • If you're worried about needles, don't be-most blood donors compare the experience to a mild, split-second pinch! (
  • Platelet donors like Matthew have the potential to donate 3 units at each visit - and over the years Matthew's units have helped make a difference in the lives of thousands of patients. (
  • Because the platelet donation takes more time, donors might watch a movie, read a book, listen to music, or just simply relax. (
  • Donors are encouraged to give where they're needed most depending on their blood type. (
  • So there's always a need for blood donors. (
  • About 15% of blood donors are high school and college students. (
  • Observances like World Blood Donor Day on June 14 are a time to thank donors and remind people of the importance of blood donation. (
  • The app also records an individual's donation history, blood type, and notifies donors of the results of their blood screening. (
  • The U.S. Food and Drug Administration ensures the safety of blood donations and protects the health of donors. (
  • We take for granted that blood will be there at the hospital when we need it, but it can only come from generous volunteer donors. (
  • Blood and platelet donors can save time at their next donation by using RapidPass ® to complete their pre-donation reading and health history questionnaire online, on the day of their donation, from a computer or mobile device. (
  • While those with cancer, HIV, or disease-causing bacteria in the blood or body tissue are usually overlooked as donors, there are instances where they can donate. (
  • It is used in low doses to inhibit platelet aggregation and improve complications of venous stases and thrombosis. (
  • It inhibits the primary and secondary phase of aggregation induced by adenosine 5'-diphosphate (ADP) and reduces platelet-derived growth factor. (
  • Dipyridamole potentiates the inhibitory effects of aspirin on platelet aggregation. (
  • Effects of keishibukuryogan (KBG) on platelet aggregation were investigated. (
  • To ensure the specificity of KBG, tokishakuyakusan (TSS) and kamisyoyosan (KSS), which are known to have platelet aggregation-inhibiting effects, and rikkunshito (RKT) and shakuyakukanzoto (SKT), which are considered to be devoid of such effects, were used for comparison. (
  • The platelet aggregation of each test drug was measured by the screen filtration pressure method using whole blood of guinea pigs and expressed as a collagen-induced pressure rate (%) or a collagen concentration required for 50% increase in the pressure rate (PATI value). (
  • Furthermore, paeonol, a representative component of Moutan cortex, and aspirin which is known to have platelet aggregation-inhibiting activity (COX-1 inhibitor) also showed similar effects. (
  • These results suggest that the platelet aggregation-inhibiting activity of the constituent crude drugs Moutan cortex and Cinnamomi cortex is involved in the improving effects of KBG on impaired microcirculation and that paeonol plays a role in these effects. (
  • It has been suggested that platelet aggregation-inhibiting activity is involved in the improving effects of TSS and KSS on microcirculatory disturbances [ 5 ]. (
  • However, the effects of KBG on platelet aggregation have been little investigated [ 6 ], although increased blood viscosity and reduced erythrocyte deformability are reported to be involved in the antioketsu effects [ 3 , 7 ]. (
  • While platelet-rich plasma (PRP) has been used in platelet aggregation tests [ 8 ], PRP has been shown to have effects on the platelet activation and the sensitivity to coagulation stimuli because it is adjusted by centrifugation used in its preparation from whole blood. (
  • For this reason, attempts are being made to directly test platelet aggregation using whole blood [ 9 - 12 ]. (
  • Moreover, platelet aggregation measured with guinea pig or mouse whole blood has been shown to be similar to that measured with human whole blood [ 13 ]. (
  • In this study, we used the screen filtration pressure (SFP) method [ 9 - 11 , 13 , 14 ] with guinea pig whole blood to investigate the mechanistic involvement of platelet aggregation-inhibiting action in the microcirculation disorder-improving effects of KBG and to identify active crude drugs and components contributing to these effects. (
  • In functional assays, oxLDL abolished guanosine 39,59-cyclic monophosphate (cGMP)- mediated signaling and inhibited platelet aggregation and arrest under flow. (
  • We determined the effect of flavonolignans on arachidonic acid induced blood platelet aggregation, COX pathway metabolites formation, as well as COX activity in platelets. (
  • We observed that tested compounds decrease the platelet aggregation level, both thromboxane A 2 and malondialdehyde formation, as well as inhibit the COX activity. (
  • TXA 2 is a very strong blood platelet activator acting as a pro-aggregator and vasoconstrictor mediator, leading to increased platelet aggregation. (
  • These chemicals attract other platelets to the site of the injury and make them clump together to form what is called a platelet plug (this process is called aggregation). (
  • A laboratory study on human blood revealed that PPA used in conjunction with aspirin may potentiate decreased platelet aggregation. (
  • Blood is made up of various types of cells, namely red blood cells, white blood cells and platelets, also known thrombocytes. (
  • Platelets, also known as thrombocytes, are made in the bone marrow and are present in the blood to help it clot. (
  • Platelets - Thrombocytes. (
  • According to Dr. Preeti Jain, Senior Dietitian, Action Cancer Hospital, New Delhi, ''Food to increase blood platelets counts can include vitamin B-12, folate, vitamin C and iron. (
  • 4. Vitamin B-12 Vitamin B-12 may help keep your blood cells healthy and its deficiency has been associated with low platelet counts. (
  • Some guidelines specify target platelet counts. (
  • In clinical practice the recommended target platelet counts may not be achieved even with large doses of platelets. (
  • What are platelet counts? (
  • Platelet counts are a method of measurement of the number of platelets per microliter of your blood. (
  • When you want to increase platelet counts, then fatty fish because it's high in vitamin B12. (
  • Laboratory findings include low white blood cell and platelet counts and elevated liver enzymes. (
  • Moreover, quantification methods to assess the manual blood counts can be done by examining multiple fields. (
  • Your child's blood counts will be monitored periodically throughout treatment, and if some or all of the blood counts are too low, chemotherapy might be held until the counts come back up, or you might be advised to take special precautions to avoid infections or injuries. (
  • A more severe form of dengue, called dengue hemorrhagic fever, is characterized by high fever, low blood platelet counts, plasma leakage, and hemorrhagic manifestations. (
  • A repeat with haemoglobin and platelet counts being normal (140 test for dengue IgM/IgG on day 8 of il ness was positive g/L and 156 × 109/L, respectively). (
  • Platelet and blood vessel disorders. (
  • Blood clot contraction plays an important role in prevention of bleeding and in thrombotic disorders. (
  • Contraction of blood clots and thrombi is an interdisciplinary problem related to fundamental aspects of cell biology, including cell motility and interaction of cells with extracellular matrix, as well as to blood clotting and its disorders, such as heart attack, stroke, and venous thromboembolism. (
  • This test is indicated for assessing platelet ultra-structural abnormalities in congenital and acquired platelet disorders. (
  • Patients with either hereditary or acquired platelet disorders usually have bleeding diathesis, which can potentially be life threatening. (
  • Platelet (P) transmission electron microscopy (TEM) has been an essential tool for laboratory diagnosis of various hereditary platelet disorders since it was first used to visualize fibrin-platelet clot formation in 1955. (
  • Oxidized low-density lipoprotein (oxLDL) promotes unregulated platelet activation in dyslipidemic disorders. (
  • They, therefore, have the potential to be a powerful tool in therapy for blood-related illnesses, such as malaria and blood-clotting disorders. (
  • Donating platelets is a simple way to make a profound difference in the lives of patients that depend on the tiny cells that work to form clots and prevent life-threatening bleeding and help those living with blood disorders. (
  • Blood-clotting problems can indicate various disorders like excessive clotting disorder, bone marrow disease or bleeding disorder. (
  • RMS due to blood protein or platelet defects may come about through either of two mechanisms: (1) disorders associated with a hemorrhagic tendency or (2) defects associated with a thrombotic tendency. (
  • What are platelet function disorders? (
  • Platelet function disorders are conditions in which platelets do not work the way they should, resulting in a tendency to bleed or bruise. (
  • Since platelets have many roles in blood clotting, platelet function disorders can lead to bleeding disorders of various intensities. (
  • Storage pool deficiencies are a group of disorders caused by problems with platelet granules. (
  • Blood disorders can manifest as clinical signs and symptoms that can be similar to many other medical conditions, hence the beauty of incorporating the patient's medical history and physical examination to reach an accurate diagnosis. (
  • You can trust our specialists to treat your child with expertise and compassion as they diagnose, treat and prevent various blood disorders. (
  • Early detection through advanced diagnostic tests and treatment of blood disorders are vital to patient outcomes. (
  • Our compassionate, dedicated and knowledgeable pediatric hematologists provide early detection, diagnoses and treatment plans specific to your child's unique blood disorders and needs. (
  • Bleeding disorders are conditions in which the blood does not clot normally because certain proteins in the blood are missing or do not work properly. (
  • The bleeding disorder community includes people living with von Willebrand disease (VWD) , hemophilia , platelet disorders, and other rare bleeding disorders (called rare factor deficiencies). (
  • In addition, the disturbances are also caused by various factors such as increased blood viscosity, active oxygen species, decreased erythrocyte deformability, coagulation/fibrinolysis system, and adhesion factors. (
  • Blood platelets are the smallest, un-nucleated morphotic elements of human blood that play a major role in the blood coagulation system. (
  • IMSEAR at SEARO: Platelet function and blood coagulation in tetanus. (
  • They are mainly involved in blood coagulation. (
  • Haemostasis and thrombosis rely on three components namely the vascular endothelial wall, blood platelets and the coagulation cascade. (
  • Platelets form clots when an individual's blood vessel undergoes any damage. (
  • You donate the tiny cells in your blood that form clots. (
  • When Chelsee was 7 years old, she was diagnosed with platelet storage pool disorder, a condition where parts of platelets (blood cells that help the blood form clots) are absent, reduced, or unable to enter the blood stream, impacting the platelets to form clots and stop a bleed. (
  • Her doctor called to let her know that her platelets (blood cells that help form clots to stop bleeding) were extremely low and that she needed to go to the emergency department right away. (
  • 2. Folate rich foods Deficiency of folate in the body may lead to a lower blood platelet count. (
  • When a blood vessel is damaged, platelets stick to the edges of it, clustering together to plug the hole. (
  • If the injury to the vessel is large, platelets alone will not stop the bleeding, so they release other factors that start blood clotting. (
  • When a blood vessel becomes damaged, it sends a signal to the platelets, which respond by traveling to the damaged area. (
  • Here, they transform into their active shape, growing tentacles so that they can adhere to the broken blood vessel to plug the bleeding. (
  • How they do is this is to use cells from blood vessels that exist to make new blood vessel tissue, or angiogenesis. (
  • It is used both angiogenesis, and vasculogenesis (the creation of new blood vessel tissue). (
  • When a blood vessel is injured, platelets stick to the damaged area and spread along the surface to stop the bleeding (this process is called adhesion). (
  • Because this receptor is absent or is not working properly, platelets do not stick to the injured blood vessel wall the way they should and it is difficult for the normal blood clot to form. (
  • Without these chemicals, platelets are not activated properly and the injured blood vessel does not constrict to help stop bleeding. (
  • Without these proteins, platelets cannot stick to the blood vessel wall, clump together the way they should, or repair the injured blood vessel. (
  • Platelets stop bleeding by clumping and forming plugs in blood vessel injuries. (
  • The platelet donation process follows the same steps as whole blood donation registration, screening, donation and after-care. (
  • Ask us about donating platelets at your next whole blood donation or give us a call at 1 888 2 DONATE (1-888-236-6283). (
  • How long does a platelet donation take? (
  • The plateletpheresis process is usually about 75 to 100 minutes for a large-volume donation and about 50 to 75 minutes for a single-unit donation, depending on your platelet count. (
  • Large-volume donations collect two times more platelets than a single donation. (
  • One large volume donation makes twice as much transfusable platelet units. (
  • Since our bodies manufacture and store extra platelets, the platelets collected during your donation is replaced almost immediately. (
  • If you do wish to donate both blood and platelets, you need to wait 56 days after either type of donation before making the other. (
  • You're welcome to try different donation programs to see which you like best: blood, plasma or platelets. (
  • If you are thinking of making your first platelet donation or want to book an appointment, call your local donor centre. (
  • Is it possible to draw only platelets from a donation? (
  • As with a whole blood donation, we insert a needle into your arm and draw blood from your body. (
  • Because of this constant and critical demand, platelets are one of the most needed donation types. (
  • Platelet donation requires additional criteria to ensure you're a good candidate for this type of donation. (
  • Platelet donation is done through apheresis, a process in which blood is removed, spun through a machine to remove the needed part, and then returned to the body. (
  • Whole blood donations are the most common form of donation. (
  • This blood type allows medical facilities to give the right blood to the right patient, as well as helping blood centers find the best donation for your type. (
  • Are you eligible for blood donation? (
  • New to Blood Donation? (
  • Enter your information to learn the truth about some blood donation myths and how you can help patients. (
  • The process takes longer than a typical blood donation, about two hours, but those donations are incredibly important because platelets have a very short shelf life of only 5 days. (
  • For every blood donation made during the month of April, the Rhode Island Blood Center will contribute one dollar to help Ukrainian refugees. (
  • Our platelet donation clinics are in Dublin and Cork. (
  • If you have any questions regarding platelet donation please contact: 01-4322833 or 021- 4807400 for the Munster region. (
  • Preliminary results are available in minutes at your first blood donation. (
  • For the last 30-plus years, Matthew Murphy II has made a point to share his good health and donate platelets every two weeks at the American Red Cross Laguna Hills Blood Donation Center. (
  • Recently, the staff joined together to celebrate Matt's 1,600th-platelet unit donation milestone , a milestone few others have achieved. (
  • Matthew's blood donation journey began in his 20s. (
  • To learn more about platelet donation, visit . (
  • There are only 5 days after someone gives a (platelet) donation for it to be transfused to a patient," emphasizes Jecoliah Ellis, External Communications Manager for the American Red Cross of Massachusetts. (
  • A platelet donation is also known as "apheresis" or "platelet pheresis. (
  • According to the Red Cross, your platelet donation may potentially provide up to 3 single doses that can be used for 3 separate patients, or up to 3 doses that can be used for a single patient. (
  • It could potentially take a total of 12-18 blood donations to provide the equivalent of one platelet donation. (
  • Like a blood donation, more than a third of the U.S. population is eligible to donate platelets. (
  • Preparing to give platelets is the same as donating blood: stay hydrated and eat a light, healthy meal on the day of your donation. (
  • Platelet donations can only be given at Blood Donation Centers. (
  • Then, it is on to the actual donation where the blood is draw from your arm into the cell separator. (
  • To keep the blood supply safe, every donation is tested for blood type and checked for infectious diseases. (
  • One blood donation can save up to three lives. (
  • This is the most common and flexible type of donation where they simply take approximately one pint of your blood. (
  • You give a concentrated donation of red blood cells which can have a greater impact on patients. (
  • These donations can only be done at Red Cross donation centers, not at blood drives. (
  • Blood donations can occur at a blood bank, blood donation center, mobile facility, or hospital. (
  • Plasma Donation Centers Biolife Plasma Services Donating Whole Blood earns you extra money and helps you to save lives. (
  • Whatever your COVID-19 vaccination status, you are ABSOLUTELY allowed to give blood or platelets. (
  • Right now, the Red Cross asks eligible individuals to give blood or platelets to help meet the everyday needs of hospitals and patients, including survivors of trauma, people with cancer , and people with sickle cell disease . (
  • Aspirin inhibits prostaglandin synthesis, preventing formation of platelet-aggregating thromboxane A 2 . (
  • The effect of aspirin on platelet function can be assessed by optical platelet aggregometry or Platelet Function Analyzer (PFA-100). (
  • I was prescribed a daily aspirin, and ended up in the ER last week vomiting blood. (
  • If you're going to donate platelets, do not take aspirin products for two days prior to your appointment. (
  • Decrease of chloride concentration contributes to cardiovascular diseases, however, whether decrease of chloride concentration is involved in platelet activation remains elusive. (
  • Patient-specific red blood cells and platelets derived from iPS cells, which would solve problems related to immunogenicity and contamination, could potentially be used therapeutically, and decrease the anticipated shortage and the need for blood donations," added Murphy. (
  • Why Does The Platelet Count Decrease? (
  • The decrease in the white blood cells count in the human blood is known as Leukopenia. (
  • Regular blood tests for platelet count for individuals who are diagnosed with blood clotting conditions allow doctors to check the effectiveness of medication on the increase or decrease of blood's ability to form a clot. (
  • However, a decrease of platelets in the body can cause many symptoms such as fatigue, bleeding gums, easy bruising, and so on. (
  • CHARLOTTE, N.C. (July 20, 2017) - A nationwide analysis of 645 hospitals found a 20 percent decrease in blood utilization across 134 diagnoses that account for 80 percent of red blood cell use, according to Premier Inc. The analysis illustrates the strength of having comparative data analytics to drive performance improvement. (
  • Patients who have low platelets or platelets that don't function properly (e.g. cancer patients undergoing chemotherapy) need platelet transfusions as part of their treatment. (
  • Patients who receive multiple platelet transfusions sometimes produce antibodies that fight the donated platelets and prevent them from performing their clotting function. (
  • Platelets are critically needed every day for some of the sickest patients, like those in cancer treatment or babies born without clotting factors. (
  • To determine the effectiveness and safety of transfusing patients with severe trauma and major bleeding using plasma, platelets, and red blood cells in a 1:1:1 ratio compared with a 1:1:2 ratio. (
  • Blood product ratios of 1:1:1 (338 patients) vs 1:1:2 (342 patients) during active resuscitation in addition to all local standard-of-care interventions (uncontrolled). (
  • Cancer patients, trauma patients, and people with other health conditions need platelet infusions as part of their medical treatments. (
  • They're most often needed by cancer patients, like a 5 year old from Lincoln named Rae, who receives platelet transfusions donated at RIBC. (
  • Cancer treatments make it difficult for patients to produce their own platelets. (
  • Platelets are also crucial in treating trauma patients, women experiencing complications during child birth, and patients with other health concerns that affect their ability to produce their own platelets. (
  • Platelets are used in the treatment of cancer and leukemic patients, bone marrow transplant, new born babies and burns victims. (
  • Platelets are needed every day for the treatment of patients with cancer, leukaemia, premature babies, major surgery, burns patients, accident victims who have had extensive injury and new born babies. (
  • Many of Ireland's patients rely on platelets, so it is important supplies are constantly replenished.Platelets are essential to enable blood to clot properly and are a component of blood. (
  • Patients who do not have enough platelets in their blood are at risk of spontaneous bleeding. (
  • A reliable laboratory diagnosis of a platelet disorder can significantly impact patients' and, potentially, their family members' clinical management and outcome. (
  • This method could provide red blood cells and platelets for research and therapies, while also reducing the need for blood donations to treat patients requiring blood transfusions. (
  • Thanks to Matt's unwavering commitment to donating over the last 30 years, the team ceremonially crowned him for his record as the top Red Cross platelet donor in Southern California and recognized his generous efforts to help thousands of hospital patients. (
  • Years later, during the 1991 KLOS Blood Drive, I found out about platelets and realized how many more people I could help out throughout the year including cancer patients, new-born babies, and others that needed the product. (
  • About 2 million units of platelets are transfused each year in the U.S., and more than half of all donated platelets go to cancer patients. (
  • Here, we show that platelet-neutrophil complexes (PNCs) are increased in patients with acute myocardial infarction and that this is associated with increased levels of neuronal guidance protein semaphorin 7A (SEMA7A). (
  • For many cancer patients, burn victims, and bone marrow recipients, platelets are a life-saving therapy. (
  • Wantai, Coutaboeuf, France) were negative at Hepatitis E virus (HEV) infection causes acute liver disease, that time, but a blood sample taken on day 75 posttrauma but severe infections are rare in immunocompetent patients. (
  • RDW and MPV, tests commonly contained within routine complete blood count (CBC), may be a cost-effective manner to identify PsO and PsA patients at increased risk of MACE. (
  • Despite skepticism from his colleagues, Freireich believed that leukemia patients bled to death due to a lack of blood-dwelling cell fragments called platelets. (
  • He found that mixing platelets from his own blood with leukemia patients' blood made it clot normally, and infusions of platelets into patients stopped the bleeding. (
  • Most patients, even if the white blood cell count is very low, do not show any symptoms and oral antibiotics at home are the only treatment needed. (
  • He says the nation's need for blood donations is constant, "Each day, the Red Cross must collect nearly 13,000 blood donations for patients at about 2,500 hospitals nationwide. (
  • You donate the part of your blood used to treat patients in emergencies. (
  • Subject to patent application filed by University of Birmingham Enterprise, the research also showed that the organoids provide a micro-environment that can accept and support the survival of cells from patients with blood malignancies, including multiple myeloma cells, which are notoriously difficult to maintain outside the human body. (
  • Bone marrow fibrosis can develop in patients with certain types of blood cancers and remains incurable. (
  • Dr Psaila added "We hope that this new technique will help accelerate the discovery and testing of new blood cancer treatments, getting improved drugs for our patients to clinical trials faster. (
  • Every two seconds in the United States blood is needed to respond to patient emergencies, including accident and burn victims, heart surgery and organ transplant patients, and those receiving treatment for leukemia, cancer or sickle cell disease. (
  • The Red Cross must collect more than 13,000 blood and platelet donations every day for the patients at about 2,600 hospitals nationwide, including 80 hospitals in Indiana. (
  • All blood types are needed to ensure a reliable supply for patients. (
  • These medicines are not generally given to patients with blood cancers such as leukemia. (
  • Bringing expertise and compassion to pediatric patients, our dedicated pediatric hematologists effectively diagnose, treat and prevent various common and rare blood-related conditions facing children. (
  • Many patients with Hermansky-Pudlak syndrome have reduced von Willebrand factor activity in their platelets. (
  • There are some reports that these patients lack the CD63 marker on platelets. (
  • Sample Population -Segments of digital blood vessels obtained from 6 healthy mixed-breed horses and ponies euthanatized at an abattoir and platelets isolated from 4 healthy ponies. (
  • Their primary role is to form "plugs" that stop bleeding from injured blood vessels. (
  • They are cell fragments that supplement growth and protect damaged blood vessels from bleeding, and help recover from internal and external physical bruises. (
  • Bone marrow is found in the center of most bones and has many blood vessels. (
  • It's use is to help blood vessels grow. (
  • If your body is injured, this growth factor will come into play and help your blood vessels recover by creating new tissue. (
  • It is also useful in creating muscle tissue, as well as creating new blood vessels to bypass ones blocked by atherosclerosis. (
  • They are involved in the formation of blood clots and the repair of damaged blood vessels. (
  • A cross section of a mini bone marrow organoids showing cells that produce blood platelets, in a network of blood vessels. (
  • Maintains healthy blood flow through your capillaries-the most fragile of your blood vessels. (
  • [ 3 ] For example, in pneumococcal HUS, S pneumoniae damages endothelial cells in the blood vessels, disturbing local complement homeostasis and producing a thrombogenic state. (
  • Blood platelets are formed in bone marrow when a parent cell, a megakaryocyte, sends out arm-like extensions called proplatelets. (
  • When a person has dengue, the structure of his bone marrow is compromised which means his blood cells are compromised. (
  • Adult acute myeloid leukemia (AML) is a type of cancer in which the bone marrow makes a large number of abnormal blood cells. (
  • Tests that examine the blood and bone marrow are used to diagnose AML. (
  • Normally, the bone marrow makes blood stem cells (immature cells ) that become mature blood cells over time. (
  • Leukemia cells can build up in the bone marrow and blood so there is less room for healthy white blood cells, red blood cells, and platelets. (
  • The bone marrow produces platelets, which are blood cells that help stop bleeding by promoting blood clotting. (
  • Red cell distribution width (RDW) and mean platelet volume (MPV) are readily available clinical tests that reflect responses of the bone marrow and/or plasma thrombogenicity (e.g., inflammation), and can be markers for major adverse cardiac events (MACE). (
  • Bone marrow is a spongy material inside the bones that makes white blood cells, red blood cells, and platelets. (
  • With leukemia (loo-KEE-mee-uh), the bone marrow makes white blood cells that don't work. (
  • This happens because the bone marrow stops making the usual amount of red blood cells. (
  • By looking carefully at the blood or bone marrow, doctors check for changes in the genes. (
  • For starters, bone marrow contains a type of stem cell that produces the body's red blood cells, white blood cells and platelets. (
  • In myelodysplastic syndrome (MDS), a slowly developing form of cancer that can devolve into acute leukemia, abnormal stem cells reproduce within the bone marrow, where they die prematurely -- decreasing their ability to produce red and white blood cells and platelets. (
  • When the bone marrow if affected, we can see a low white blood cell or platelet count. (
  • This life-like architecture enabled the team to study how the cells in the bone marrow interact to support normal blood cell production and how this is disturbed in bone marrow fibrosis (myelofibrosis), where scar tissue builds up in the bone marrow, causing bone marrow failure. (
  • Senior study author Professor Bethan Psaila, a haematology medical doctor as well as a research Group Leader at the Radcliffe Department of Medicine, University of Oxford, said "To properly understand how and why blood cancers develop, we need to use experimental systems that closely resemble how real human bone marrow works, which we haven't really had before. (
  • in our ongoing collaborative work we will be working with others to better understand how the bone marrow works in healthy people, and what goes wrong when they have blood diseases. (
  • A study, published in the journal Cancer Discovery, describes the new method, which results in an organoid that faithfully models the cellular, molecular and architectural features of myelopoietic (blood cell producing) bone marrow. (
  • Myelosuppression is the technical term for the suppression of the bone marrow's production of blood cells: red blood cells, white blood cells, and platelets. (
  • At Pediatrix, our board-certified, fellowship-trained pediatric hematologists are experienced in treating the hematological system, which includes red and white blood cells, platelets, the spleen and bone marrow. (
  • The ultrastructure of ceroid-lipofuscin inclusions in macrophages in bone marrow, gut, and other tissues can be helpful, but the absence of dense granules in the platelets in association with albinism is diagnostic for Hermansky-Pudlak syndrome. (
  • Over 22,000 platelet transfusions are needed every year in Ireland. (
  • If an injury or blood loss occurs, platelets are released, and a person's blood begins to clot to prevent excessive bleeding. (
  • This is a technique of withdrawing just the single component - the platelet, through a cell-separating machine, and returning the red cells and plasma back to a person's body. (
  • Having any of these markers (or none of them) doesn't make a person's blood any healthier or stronger. (
  • A person's platelet count can fluctuate during menstruation and become low during the later stages of pregnancy. (
  • We take you through the symptoms and causes of low platelet count and the foods to increase blood platelets. (
  • Consult your doctor immediately in case you have low blood platelet count and experience headaches or any neurological problem. (
  • Add more vitamin B9 or folate rich foods that may be extremely important for healthy cell division in the body that can help increase blood platelet count. (
  • Approximately 25% of these babies have a low platelet count and require platelet transfusions. (
  • The outcomes of the babies in each arm were monitored and the differences compared to determine whether a platelet count of 25 or 50 x109/L was better. (
  • Every dengue patient is recommended to take the Complete Blood Count (CBC) test every day in order to check the platelet count level, especially in the later stages of the fever. (
  • Normal blood platelet count should be around 150,000 to 450,000/cmm in humans. (
  • The most common myth about platelets is that a fall in its count means dengue. (
  • Another myth is that a dengue patient with a dropping platelet count must be hospitalized immediately. (
  • However, if this drop in platelet count is accompanied by active bleeding then it is a symptom of the Dengue Haemorrhagic Fever and should not be ignored at any cost. (
  • How To Increase Your Blood Platelet Count? (
  • It is advisable to do it when the platelet count is less than 10,000 and the patient exhibits active bleeding. (
  • What is PLT Count Blood Test? (
  • A platelet count in blood tests means measuring the number of platelets present in an individual's body. (
  • Individuals willing to know more about PLT count blood tests can continue reading. (
  • However, an individual's platelet count can change with age and other medical conditions. (
  • The normal range for the platelet count in peripheral blood at all ages is 150-400 x 109/l. (
  • You need to consult your doctor promptly if you have a low blood platelet count and experience headaches or any neurological issues. (
  • The following are the food items that can help you to increase your blood platelet count. (
  • Salmon, trout, and tuna are all filled with B12 punch, so add these fatty fishes to your meal to help boost your platelet count. (
  • Doctors consider a healthy platelet count to fall within the range of 150,000-400,000 per microliter (μl) of blood. (
  • People are more at risk of bleeding the lower their platelet count drops. (
  • However, bleeding problems are unlikely until the platelet count is less than 100,000 platelets per μl. (
  • A platelet count blood test is important for the calculation of platelet in the blood. (
  • The following five are the requirements for platelet count blood tests. (
  • How to increase platelet count in dengue? (
  • Tests such as a complete blood count , liver function and kidney function panels, and blood chemistry tests can give important information about the number of normal blood cells in the body and how well the organs are working. (
  • Dangerous internal bleeding can occur when your platelet count follows below 10,000 or below 10 platelets per microliter. (
  • I have blood work done every three months, but my white count keeps dropping. (
  • If the white blood cell count is very low and your pet has a fever or feels sick, a stay in the hospital for a day or two on IV fluids and antibiotics until they are feeling better may be needed, although this is rare. (
  • The white blood cell count is routinely checked one week after the treatment, when the white blood cell count is at its lowest, to monitor for these problems and be proactive in treating this side effect. (
  • ANC is the percentage of neutrophils (segs and bands added together) multiplied by the total white blood count. (
  • His laboratory investigations revealed hemoglobin of 8.9 g/dl and normal white blood cell (WBC) and platelet count. (
  • Standard blood tests (eg, prothrombin time [PT], activated partial thromboplastin time [aPTT], platelet count, bleeding time) do not identify the platelet defect in Hermansky-Pudlak syndrome. (
  • The complete blood count initial y revealed leukopenia extremity, with some areas of erythematous petechial at 3.16 × 109/L (neutrophils 75%, lymphocytes 18%), confluence and islands of normal skin ( Fig. 2 ). (
  • Assuming an improvement of wastage by 10% and an average platelet unit price of €250 to €600, increased shelf life could save €25 to €60 per unit. (
  • Under arterial flow, oxLDL triggered sustained generation of platelet intracellular ROS, which was blocked by CD36 inhibitors, mimicked by CD36-specific oxidized phospholipids, and ablated in CD362/2 murine platelets. (
  • After the platelets' activation, signal transduction leads to mobilization of intracellular calcium ions (Ca 2+ ). (
  • When they don't have enough platelets (PLATE-lits), kids with leukemia may bruise easily, get nosebleeds, or bleed for a long time after even a minor cut. (
  • This means that you do not have anti-platelet antibodies in your blood. (
  • Abnormal results show that you have anti-platelet antibodies. (
  • Inhibition of COX activity is one of the major means of anti-platelet pharmacotherapy preventing arterial thrombosis and reducing the incidence of cardiovascular events. (
  • One of the major method in anti-platelet pharmacotherapy of preventing arterial thrombosis is inhibition of COX activity. (
  • This was prevented by either pharmacologic inhibition of NOX2 in human platelets or genetic ablation of NOX2 in murine platelets. (
  • August 11, 2005 - In a discovery that upends a longstanding tenet of human biology, University of Utah School of Medicine researchers have shown that a key process in gene regulation can occur in human platelets, unique cells that are unusual because they don't have a nucleus (anucleate). (
  • Vitamin A is essential for a healthy platelet production. (
  • This system is called Individual Donor Risk Assessment and is similar to the system that was introduced in the UK Blood Services in 2021. (
  • Retrieved from . (
  • (label-accessed February 04, 2023). (
  • In contrast to other cell-matrix systems in which cells migrate along fibers, the "hand-over-hand" longitudinal pulling causes shortening and bending of platelet-attached fibers, resulting in formation of fiber kinks. (
  • Platelets are about a quarter of the size of red blood cells and are not whole cells but rather fragile cell fragments. (
  • Platelets are tiny disk-shaped fragments of blood cells that are essential for normal blood clotting. (
  • In microcirculatory disturbances, endothelial cells of the vascular wall are damaged, and leukocytes or platelets agglutinate on endothelial cells to form a clot. (
  • An unlimited number of red blood cells and platelets can be generated from induced pluripotent stem (iPS) cells in vitro , according to a recent study out of the Boston University School of Medicine (BUSM) and School of Public Health (BUSPH), funded in part by NIEHS. (
  • Previous research has shown that iPS cells can be generated by reprogramming normal adult cells, such as skin or blood cells, into a more primitive stem cell state. (
  • From this stem cell state, they can then be pushed to differentiate into other cell types, such as hematopoietic cells, which are blood cell precursors. (
  • In this study, researchers used growth factors and a patented technology to push iPS cells to differentiate into red blood cells and platelets. (
  • In this study, modulating the AhR receptor in the iPS cells drove an unprecedented rapid increase in the number of new red blood cells and platelets in vitro . (
  • When created from a patient's own cells, these iPS-derived blood cells are not viewed by the immune system as foreign material and may be used in therapies without concern for an immune response against the cells. (
  • This method of generating cells could also help offset blood supply shortages. (
  • But using stem cells from human umbilical cord blood to engineer the precursor cell that forms platelets and platelets isolated from the blood of study subjects, the Utah researchers found that splicing also takes place in the cytoplasm of circulating platelets. (
  • Finding that platelets can splice the IL-1ß pre-mRNA was completely unexpected and emerged while the researchers were engaged in earlier studies of how platelets communicate with certain leukocytes (white blood cells). (
  • Platelets are abundant cells that circulate in human blood and have many functions. (
  • Leukemia may affect red blood cells, white blood cells, and platelets. (
  • Red marrow contains blood stem cells that can become red blood cells, white blood cells, or platelets. (
  • Red blood cells that carry oxygen and other substances to all tissues of the body. (
  • Granulocytes , which are white blood cells that help fight infection and disease. (
  • In AML, the myeloid stem cells usually become a type of immature white blood cell called myeloblasts (or myeloid blasts ). (
  • The myeloblasts in AML are abnormal and do not become healthy white blood cells. (
  • Sometimes in AML, too many stem cells become abnormal red blood cells or platelets. (
  • These abnormal white blood cells, red blood cells, or platelets are also called leukemia cells or blasts. (
  • The leukemia cells can spread outside the blood to other parts of the body, including the central nervous system (brain and spinal cord ), skin, and gums . (
  • Experiments in genetically modified animals identify Sema7a on red blood cells to be crucial for this condition. (
  • The white blood cells present in the blood do the job of protecting the body against infections and diseases. (
  • Red blood cells have markers on their surface that characterize the cell type. (
  • These markers (also called antigens ) are proteins and sugars that our bodies use to identify the blood cells as belonging in us. (
  • If a patient gets the wrong blood type, the antibodies immediately set out to destroy the invading cells. (
  • If you have both A and B markers on the surface of your cells (type AB blood), your body does not need to fight the presence of either. (
  • But if you have type O blood, your red blood cells have no A or B markers. (
  • A platelet test is part of a complete blood test and measuring platelets, white blood cells and red blood cells. (
  • Platelets are red blood cells that help improve clotting that prevents severe bleeding. (
  • Platelets are essential cells that prevent the abundance of loss of blood and protect from resulting ailments. (
  • Leukemia is a cancer that mostly affects white blood cells. (
  • White blood cells (also called leukocytes) fight infections. (
  • Juvenile myelomonocytic (mye-uh-low-mon-uh-SIT-ik) leukemia (JMML) happens when immature blood cells (called blasts) make too many myelocytes and monocytes (two types of white blood cells. (
  • Eventually they may develop anemia , which is when the body has too few red blood cells. (
  • Because their white blood cells can't fight infections, kids with leukemia are more likely to get viral or bacterial infections. (
  • The shapes and sizes of the blood cells are checked with a microscope. (
  • Doctors use these to rule out other causes of symptoms, or look for a mass of leukemia cells in the chest that can affect breathing or blood circulation. (
  • It compares the proteins on the surface of the child's blood cells with the proteins on a potential donor's cells. (
  • Platelets are small cells that circulate in the blood. (
  • As you're aware, MDS often causes fatigue (caused by anemia) and a drop in white blood cells, putting people at greater risk of infection. (
  • Too much bilirubin may mean that too much is being produced, usually due to increased destruction of red blood cells (hemolysis). (
  • Together, these three kinds of blood cells add up to a total 45% of the blood tissue by volume, with the remaining 55% of the volume composed of plasma , the liquid component of blood. (
  • Haemoglobin (the main component of red blood cells) is an iron -containing protein that facilitates transportation of oxygen from the lungs to tissues and carbon dioxide from tissues to the lungs. (
  • Red blood cells primarily carry oxygen and collect carbon dioxide through the use of haemoglobin , and have a lifetime of about 120 days. (
  • They have the job alongside the white blood cells of protecting the healthy cells. (
  • This will help to assess the overall quality of the smear, abnormal red blood cell (RBC) distribution (e.g., rouleaux), and/or rapid detection of large abnormal cells (e.g., blasts). (
  • A dry look and subsequently an oil-immersed look can help in quantifying white blood cells (WBCs) and platelets to see if they align with what is reported by the hematology analyzer. (
  • Lowered levels of each of these types of blood cells lead to different side effects. (
  • Low levels of red blood cells result in anemia . (
  • Red blood cells carry oxygen to all parts of the body, and when there are too few red blood cells, body tissues do not get enough oxygen. (
  • White blood cells protect the body from invasion by pathogens, and when levels of specific types of these cells are low, a child has a lowered resistance to bacterial infection. (
  • The types of white blood cells that are important in fighting infections are called segs and bands . (
  • His serum sodium (Na) was 128 mEq/L and potassium (K) was 2.8 mEq/L. His urine sediment was bland with no proteinuria and no red blood cells and WBCs. (
  • Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. (
  • Once I start donating platelets, can I still give blood? (
  • What if I change my mind about donating platelets? (
  • Donating blood today? (
  • In college I started donating blood and felt the need to support the community. (
  • Donating blood is a simple, safe way to get involved and help save lives in your community. (
  • Donating blood is a simple, quick, and effective way for eligible individuals to get involved in their community. (
  • High-density lipoprotein modulates thrombosis by preventing von Willebrand factor self-association and subsequent platelet adhesion. (
  • The medical term for blood clots developing in the large veins of the leg or pelvis is deep vein thrombosis (DVT). (
  • Bacterial contamination of platelet components occurs because the storage temperature for platelets (22°C) may facilitate bacterial growth. (
  • However, if left untreated, DVT can result in a pulmonary embolism, which occurs when the blood clot travels from the leg to the lungs. (
  • Pre-eclampsia occurs in 2 phases: abnormal implantation of the placenta leads to impaired placental blood flow, which then induces the release of a critical placental substance into the maternal circulation. (
  • Our work provides a reference for studies working both on healthy platelets and pathological conditions affecting platelet transcriptome. (
  • The biological activity of platelets, both in physiological processes as well as under pathological conditions, is dependent on the degree of their activation. (
  • A lack of platelets in your body may cause symptoms like fatigue, bleeding gums, easy bruising, et al. (
  • Blood clots most often develop deep in leg veins, and symptoms are easily missed. (
  • 3. Regulates Blood Sugar -Turmeric is also good to maintain blood sugar levels and alleviates the high glucose level symptoms of excessive thirst, fatigue, and sudden weight loss. (
  • This is followed by vomiting, diarrhoea, rash, symptoms of impaired kidney and liver function, and in some cases internal and external bleeding (e.g., oozing from the gums, blood in the stool). (
  • Approximately 1 in 2,000-2,500 platelet units may be contaminated with bacteria (Levy, 2018). (
  • We at Adimarket are specialists in stem cell and PRP (platelet rich plasma) therapies. (
  • In a buy friction stir welding of May 15, 1939, the radioactive had accessed to be the other two therapies after platelet. (
  • 6. Prevents Blood Clots -Clots are formed when several blood platelets aggregate in the blood to form a clump. (
  • However, currently there is no standardized test used to detect bacteria in platelet units, and regardless of the method, bacterial screening of platelets is unlikely to detect all pathogens. (
  • During that investigation they found evidence of platelets making new proteins, which led them to pursue the mechanisms that are involved, said Guy A. Zimmerman, M.D., professor of internal medicine and one of the study's co-authors. (
  • The idea that blood platelets could make proteins without having a nucleus had been thought heretical," said Zimmerman, who also heads the U's Program in Human Molecular Biology and Genetics at the Eccles Institute of Human Genetics. (
  • Bernard-Soulier syndrome is a platelet function disorder caused by an abnormality in the genes for glycoprotein Ib/IX/V. These genes code for a group of linked proteins normally found on the surface of platelets, the glycoprotein Ib/IX/V receptor (also called the von Willebrand factor or VWF receptor). (
  • These genes code for a group of linked proteins normally found on the surface of platelets, the glycoprotein IIb/IIIa receptor (also called the fibrinogen receptor). (
  • Granules are little sacs inside the platelet in which proteins and other chemicals important to its function are stored. (
  • Donate Blood, Platelets or Plasma. (
  • Am I Eligible to Donate Blood? (
  • Find out about the eligibility requirements to donate blood today. (
  • PORTLAND, Ore. ( KOIN ) - With flu season ramping up, the American Red Cross is urging Oregonians to donate blood, in hopes of avoiding a shortage as the holiday season nears. (
  • Those who donate blood or platelets from Nov. 1 to 22 will receive a $10 e-gift card to a chosen merchant. (
  • Can Teens Donate Blood? (
  • Get Involved: Donate Blood. (
  • Individuals who are 17 years of age in most states (16 with parental consent where allowed by state law), weigh at least 110 pounds and are in generally good health may be eligible to donate blood. (
  • Platelet concentrates from whole-blood donations (buffy-coat) or apheresis: which one to use? (
  • Problems with severe bleeding and blood clots may occur. (
  • To find out if you are able to donate platelets take our eligibility quiz or call 432 2833 (Dublin) 021 480 7429 (Cork) to speak to one of our staff about giving platelets. (
  • However, platelets have the shortest shelf life of all blood components, lasting only 5 to 7 days. (
  • The ability of von Willebrand factor (VWF) to initiate platelet adhesion depends on the number of monomers in individual VWF multimers and on the self - association of individual VWF multimers into larger structures. (
  • Am I eligible to donate platelets? (
  • Using a donor's individual behaviours to determine if that person is eligible to give blood makes the process fairer and more inclusive, while maintaining the safety of the blood supply. (
  • Thank you for your interest in becoming a platelet donor, unfortunately you are not eligible as you must be under 60 years of age to become a platelet donor. (
  • Having a personal history of a blood disorder such as myelodysplastic syndrome . (
  • Glanzmann thrombasthenia is a platelet function disorder that is caused by an abnormality in the genes for glycoproteins IIb/IIIa. (
  • Delta storage pool deficiency is a platelet function disorder caused by a lack of dense granules and the chemicals normally stored inside them. (
  • Grey platelet syndrome is a very rare platelet function disorder caused by a lack of alpha granules and the chemicals normally stored inside them. (