A lipid cofactor that is required for normal blood clotting. Several forms of vitamin K have been identified: VITAMIN K 1 (phytomenadione) derived from plants, VITAMIN K 2 (menaquinone) from bacteria, and synthetic naphthoquinone provitamins, VITAMIN K 3 (menadione). Vitamin K 3 provitamins, after being alkylated in vivo, exhibit the antifibrinolytic activity of vitamin K. Green leafy vegetables, liver, cheese, butter, and egg yolk are good sources of vitamin K.
A family of phylloquinones that contains a ring of 2-methyl-1,4-naphthoquinone and an isoprenoid side chain. Members of this group of vitamin K 1 have only one double bond on the proximal isoprene unit. Rich sources of vitamin K 1 include green plants, algae, and photosynthetic bacteria. Vitamin K1 has antihemorrhagic and prothrombogenic activity.
Cell membrane glycoproteins that are selectively permeable to potassium ions. At least eight major groups of K channels exist and they are made up of dozens of different subunits.
An enzyme that catalyzes the active transport system of sodium and potassium ions across the cell wall. Sodium and potassium ions are closely coupled with membrane ATPase which undergoes phosphorylation and dephosphorylation, thereby providing energy for transport of these ions against concentration gradients.
A member of the alkali group of metals. It has the atomic symbol Na, atomic number 11, and atomic weight 23.
A class of drugs that act by inhibition of potassium efflux through cell membranes. Blockade of potassium channels prolongs the duration of ACTION POTENTIALS. They are used as ANTI-ARRHYTHMIA AGENTS and VASODILATOR AGENTS.
Phosphotransferases that catalyzes the conversion of 1-phosphatidylinositol to 1-phosphatidylinositol 3-phosphate. Many members of this enzyme class are involved in RECEPTOR MEDIATED SIGNAL TRANSDUCTION and regulation of vesicular transport with the cell. Phosphatidylinositol 3-Kinases have been classified both according to their substrate specificity and their mode of action within the cell.
A cardioactive glycoside consisting of rhamnose and ouabagenin, obtained from the seeds of Strophanthus gratus and other plants of the Apocynaceae; used like DIGITALIS. It is commonly used in cell biological studies as an inhibitor of the NA(+)-K(+)-EXCHANGING ATPASE.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Hemorrhage caused by vitamin K deficiency.
An essential amino acid. It is often added to animal feed.
An element that is an alkali metal. It has an atomic symbol Rb, atomic number 37, and atomic weight 85.47. It is used as a chemical reagent and in the manufacture of photoelectric cells.
Established cell cultures that have the potential to propagate indefinitely.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A potassium-selective ion channel blocker. (From J Gen Phys 1994;104(1):173-90)
Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.
A protein-serine-threonine kinase that is activated by PHOSPHORYLATION in response to GROWTH FACTORS or INSULIN. It plays a major role in cell metabolism, growth, and survival as a core component of SIGNAL TRANSDUCTION. Three isoforms have been described in mammalian cells.
The study of the generation and behavior of electrical charges in living organisms particularly the nervous system and the effects of electricity on living organisms.
The ability of a substrate to allow the passage of ELECTRONS.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
Potassium channels whose activation is dependent on intracellular calcium concentrations.
Addition of methyl groups. In histo-chemistry methylation is used to esterify carboxyl groups and remove sulfate groups by treating tissue sections with hot methanol in the presence of hydrochloric acid. (From Stedman, 25th ed)
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Proteins prepared by recombinant DNA technology.
Potassium channels that contain two pores in tandem. They are responsible for baseline or leak currents and may be the most numerous of all K channels.
Heteromultimers of Kir6 channels (the pore portion) and sulfonylurea receptor (the regulatory portion) which affect function of the HEART; PANCREATIC BETA CELLS; and KIDNEY COLLECTING DUCTS. KATP channel blockers include GLIBENCLAMIDE and mitiglinide whereas openers include CROMAKALIM and minoxidil sulfate.
The relationship between the dose of an administered drug and the response of the organism to the drug.
Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.
Inorganic compounds derived from hydrochloric acid that contain the Cl- ion.
One of the POTASSIUM CHANNEL BLOCKERS, with secondary effect on calcium currents, which is used mainly as a research tool and to characterize channel subtypes.
The naturally occurring or experimentally induced replacement of one or more AMINO ACIDS in a protein with another. If a functionally equivalent amino acid is substituted, the protein may retain wild-type activity. Substitution may also diminish, enhance, or eliminate protein function. Experimentally induced substitution is often used to study enzyme activities and binding site properties.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
A family of ribosomal protein S6 kinases that are considered the major physiological kinases for RIBOSOMAL PROTEIN S6. Unlike RIBOSOMAL PROTEIN S6 KINASES, 90KDa the proteins in this family are sensitive to the inhibitory effects of RAPAMYCIN and contain a single kinase domain. They are referred to as 70kDa proteins, however ALTERNATIVE SPLICING of mRNAs for proteins in this class also results in 85kDa variants being formed.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
The movement of ions across energy-transducing cell membranes. Transport can be active, passive or facilitated. Ions may travel by themselves (uniport), or as a group of two or more ions in the same (symport) or opposite (antiport) directions.
Elements of limited time intervals, contributing to particular results or situations.
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
The movement of materials across cell membranes and epithelial layers against an electrochemical gradient, requiring the expenditure of metabolic energy.
Gram-negative, non-motile, capsulated, gas-producing rods found widely in nature and associated with urinary and respiratory infections in humans.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
A subclass of symporters that specifically transport SODIUM CHLORIDE and/or POTASSIUM CHLORIDE across cellular membranes in a tightly coupled process.
A common name used for the genus Cavia. The most common species is Cavia porcellus which is the domesticated guinea pig used for pets and biomedical research.
A member of the alkali metals. It has an atomic symbol Cs, atomic number 50, and atomic weight 132.91. Cesium has many industrial applications, including the construction of atomic clocks based on its atomic vibrational frequency.
A group of slow opening and closing voltage-gated potassium channels. Because of their delayed activation kinetics they play an important role in controlling ACTION POTENTIAL duration.
Abrupt changes in the membrane potential that sweep along the CELL MEMBRANE of excitable cells in response to excitation stimuli.
The sum of the weight of all the atoms in a molecule.
A major class of calcium activated potassium channels whose members are voltage-dependent. MaxiK channels are activated by either membrane depolarization or an increase in intracellular Ca(2+). They are key regulators of calcium and electrical signaling in a variety of tissues.
Venoms from animals of the order Scorpionida of the class Arachnida. They contain neuro- and hemotoxins, enzymes, and various other factors that may release acetylcholine and catecholamines from nerve endings. Of the several protein toxins that have been characterized, most are immunogenic.
A family of protein serine/threonine kinases which act as intracellular signalling intermediates. Ribosomal protein S6 kinases are activated through phosphorylation in response to a variety of HORMONES and INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS. Phosphorylation of RIBOSOMAL PROTEIN S6 by enzymes in this class results in increased expression of 5' top MRNAs. Although specific for RIBOSOMAL PROTEIN S6 members of this class of kinases can act on a number of substrates within the cell. The immunosuppressant SIROLIMUS inhibits the activation of ribosomal protein S6 kinases.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
A subfamily of shaker potassium channels that shares homology with its founding member, Shab protein, Drosophila. They regulate delayed rectifier currents in the NERVOUS SYSTEM of DROSOPHILA and in the SKELETAL MUSCLE and HEART of VERTEBRATES.
A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.
Proteins found in any species of bacterium.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
The concentration of osmotically active particles in solution expressed in terms of osmoles of solute per liter of solution. Osmolality is expressed in terms of osmoles of solute per kilogram of solvent.
A potassium-channel opening vasodilator that has been investigated in the management of hypertension. It has also been tried in patients with asthma. (Martindale, The Extra Pharmacopoeia, 30th ed, p352)
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
A class of fibrous proteins or scleroproteins that represents the principal constituent of EPIDERMIS; HAIR; NAILS; horny tissues, and the organic matrix of tooth ENAMEL. Two major conformational groups have been characterized, alpha-keratin, whose peptide backbone forms a coiled-coil alpha helical structure consisting of TYPE I KERATIN and a TYPE II KERATIN, and beta-keratin, whose backbone forms a zigzag or pleated sheet structure. alpha-Keratins have been classified into at least 20 subtypes. In addition multiple isoforms of subtypes have been found which may be due to GENE DUPLICATION.
The muscle tissue of the HEART. It is composed of striated, involuntary muscle cells (MYOCYTES, CARDIAC) connected to form the contractile pump to generate blood flow.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
A group of enzymes which catalyze the hydrolysis of ATP. The hydrolysis reaction is usually coupled with another function such as transporting Ca(2+) across a membrane. These enzymes may be dependent on Ca(2+), Mg(2+), anions, H+, or DNA.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Single chains of amino acids that are the units of multimeric PROTEINS. Multimeric proteins can be composed of identical or non-identical subunits. One or more monomeric subunits may compose a protomer which itself is a subunit structure of a larger assembly.
Proteins found in any species of virus.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
Potassium or potassium compounds used in foods or as foods.
A mutation in which a codon is mutated to one directing the incorporation of a different amino acid. This substitution may result in an inactive or unstable product. (From A Dictionary of Genetics, King & Stansfield, 5th ed)
Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.
A condition due to decreased dietary intake of potassium, as in starvation or failure to administer in intravenous solutions, or to gastrointestinal loss in diarrhea, chronic laxative abuse, vomiting, gastric suction, or bowel diversion. Severe potassium deficiency may produce muscular weakness and lead to paralysis and respiratory failure. Muscular malfunction may result in hypoventilation, paralytic ileus, hypotension, muscle twitches, tetany, and rhabomyolysis. Nephropathy from potassium deficit impairs the concentrating mechanism, producing POLYURIA and decreased maximal urinary concentrating ability with secondary POLYDIPSIA. (Merck Manual, 16th ed)
The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The interaction of two or more substrates or ligands with the same binding site. The displacement of one by the other is used in quantitative and selective affinity measurements.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
A class of drugs that act by selective inhibition of calcium influx through cellular membranes.
A mutation caused by the substitution of one nucleotide for another. This results in the DNA molecule having a change in a single base pair.
A cyclododecadepsipeptide ionophore antibiotic produced by Streptomyces fulvissimus and related to the enniatins. It is composed of 3 moles each of L-valine, D-alpha-hydroxyisovaleric acid, D-valine, and L-lactic acid linked alternately to form a 36-membered ring. (From Merck Index, 11th ed) Valinomycin is a potassium selective ionophore and is commonly used as a tool in biochemical studies.
The level of protein structure in which regular hydrogen-bond interactions within contiguous stretches of polypeptide chain give rise to alpha helices, beta strands (which align to form beta sheets) or other types of coils. This is the first folding level of protein conformation.
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.
The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for VIRUS CULTIVATION and antitumor drug screening assays.
The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
Inorganic compounds that contain barium as an integral part of the molecule.
The nonstriated involuntary muscle tissue of blood vessels.
A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
Membrane proteins whose primary function is to facilitate the transport of positively charged molecules (cations) across a biological membrane.
A lipid phosphatase that acts on phosphatidylinositol-3,4,5-trisphosphate to regulate various SIGNAL TRANSDUCTION PATHWAYS. It modulates CELL GROWTH PROCESSES; CELL MIGRATION; and APOPTOSIS. Mutations in PTEN are associated with COWDEN DISEASE and PROTEUS SYNDROME as well as NEOPLASTIC CELL TRANSFORMATION.
An element in the alkali metals family. It has the atomic symbol Li, atomic number 3, and atomic weight [6.938; 6.997]. Salts of lithium are used in treating BIPOLAR DISORDER.
The region of an enzyme that interacts with its substrate to cause the enzymatic reaction.
Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.
Adenosine 5'-(trihydrogen diphosphate). An adenine nucleotide containing two phosphate groups esterified to the sugar moiety at the 5'-position.
A cell line generated from human embryonic kidney cells that were transformed with human adenovirus type 5.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.
A process leading to shortening and/or development of tension in muscle tissue. Muscle contraction occurs by a sliding filament mechanism whereby actin filaments slide inward among the myosin filaments.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
An serine-threonine protein kinase that requires the presence of physiological concentrations of CALCIUM and membrane PHOSPHOLIPIDS. The additional presence of DIACYLGLYCEROLS markedly increases its sensitivity to both calcium and phospholipids. The sensitivity of the enzyme can also be increased by PHORBOL ESTERS and it is believed that protein kinase C is the receptor protein of tumor-promoting phorbol esters.
The pore-forming subunits of large-conductance calcium-activated potassium channels. They form tetramers in CELL MEMBRANES.
Compounds with a core of fused benzo-pyran rings.
Process of generating a genetic MUTATION. It may occur spontaneously or be induced by MUTAGENS.
The hollow, muscular organ that maintains the circulation of the blood.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.
Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
Proteins obtained from ESCHERICHIA COLI.
A phosphatidylinositol 3-kinase subclass that includes enzymes with a specificity for 1-phosphatidylinositol, 1-phosphatidylinositol 4-phosphate, and 1-phosphatidylinositol 4,5-bisphosphate. Members of this enzyme subclass are activated by cell surface receptors and occur as heterodimers of enzymatic and regulatory subunits.
Stable elementary particles having the smallest known positive charge, found in the nuclei of all elements. The proton mass is less than that of a neutron. A proton is the nucleus of the light hydrogen atom, i.e., the hydrogen ion.
A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed)
Proteins produced from GENES that have acquired MUTATIONS.
A family of histone demethylases that share a conserved Jumonji C domain. The enzymes function via an iron-dependent dioxygenase mechanism that couples the conversion of 2-oxoglutarate to succinate to the hydroxylation of N-methyl groups.
Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.
Unstriated and unstriped muscle, one of the muscles of the internal organs, blood vessels, hair follicles, etc. Contractile elements are elongated, usually spindle-shaped cells with centrally located nuclei. Smooth muscle fibers are bound together into sheets or bundles by reticular fibers and frequently elastic nets are also abundant. (From Stedman, 25th ed)
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Use of electric potential or currents to elicit biological responses.
3 beta,5,14-Trihydroxy-19-oxo-5 beta-card-20(22)-enolide. The aglycone cardioactive agent isolated from Strophanthus Kombe, S. gratus and other species; it is a very toxic material formerly used as digitalis. Synonyms: Apocymarin; Corchorin; Cynotoxin; Corchorgenin.
Agents that inhibit PROTEIN KINASES.
Proteins transcribed from the E1B region of ADENOVIRUSES which are involved in regulation of the levels of early and late viral gene expression.
Refers to animals in the period of time just after birth.
A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
A 51-amino acid pancreatic hormone that plays a major role in the regulation of glucose metabolism, directly by suppressing endogenous glucose production (GLYCOGENOLYSIS; GLUCONEOGENESIS) and indirectly by suppressing GLUCAGON secretion and LIPOLYSIS. Native insulin is a globular protein comprised of a zinc-coordinated hexamer. Each insulin monomer containing two chains, A (21 residues) and B (30 residues), linked by two disulfide bonds. Insulin is used as a drug to control insulin-dependent diabetes mellitus (DIABETES MELLITUS, TYPE 1).
A neurotransmitter found at neuromuscular junctions, autonomic ganglia, parasympathetic effector junctions, a subset of sympathetic effector junctions, and at many sites in the central nervous system.
Compounds containing 1,3-diazole, a five membered aromatic ring containing two nitrogen atoms separated by one of the carbons. Chemically reduced ones include IMIDAZOLINES and IMIDAZOLIDINES. Distinguish from 1,2-diazole (PYRAZOLES).
Membrane transporters that co-transport two or more dissimilar molecules in the same direction across a membrane. Usually the transport of one ion or molecule is against its electrochemical gradient and is "powered" by the movement of another ion or molecule with its electrochemical gradient.
An aminoperhydroquinazoline poison found mainly in the liver and ovaries of fishes in the order TETRAODONTIFORMES, which are eaten. The toxin causes paresthesia and paralysis through interference with neuromuscular conduction.
Enzymes that catalyse the removal of methyl groups from LYSINE or ARGININE residues found on HISTONES. Many histone demethylases generally function through an oxidoreductive mechanism.
Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.
Different forms of a protein that may be produced from different GENES, or from the same gene by ALTERNATIVE SPLICING.
An optical isomer of quinine, extracted from the bark of the CHINCHONA tree and similar plant species. This alkaloid dampens the excitability of cardiac and skeletal muscles by blocking sodium and potassium currents across cellular membranes. It prolongs cellular ACTION POTENTIALS, and decreases automaticity. Quinidine also blocks muscarinic and alpha-adrenergic neurotransmission.
An adenine nucleotide containing one phosphate group which is esterified to both the 3'- and 5'-positions of the sugar moiety. It is a second messenger and a key intracellular regulator, functioning as a mediator of activity for a number of hormones, including epinephrine, glucagon, and ACTH.
Commonly observed structural components of proteins formed by simple combinations of adjacent secondary structures. A commonly observed structure may be composed of a CONSERVED SEQUENCE which can be represented by a CONSENSUS SEQUENCE.
The deductive study of shape, quantity, and dependence. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Straight tubes commencing in the radiate part of the kidney cortex where they receive the curved ends of the distal convoluted tubules. In the medulla the collecting tubules of each pyramid converge to join a central tube (duct of Bellini) which opens on the summit of the papilla.
A genetic process by which the adult organism is realized via mechanisms that lead to the restriction in the possible fates of cells, eventually leading to their differentiated state. Mechanisms involved cause heritable changes to cells without changes to DNA sequence such as DNA METHYLATION; HISTONE modification; DNA REPLICATION TIMING; NUCLEOSOME positioning; and heterochromatization which result in selective gene expression or repression.
The lower right and left chambers of the heart. The right ventricle pumps venous BLOOD into the LUNGS and the left ventricle pumps oxygenated blood into the systemic arterial circulation.
An essential amino acid that is physiologically active in the L-form.
A myeloproliferative disorder characterized by neoplastic proliferation of erythroblastic and myeloblastic elements with atypical erythroblasts and myeloblasts in the peripheral blood.
A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.
The material of CHROMOSOMES. It is a complex of DNA; HISTONES; and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell.
Striated muscle cells found in the heart. They are derived from cardiac myoblasts (MYOBLASTS, CARDIAC).
Contractile tissue that produces movement in animals.
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
Drugs used to cause dilation of the blood vessels.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
A potent vasodilator agent with calcium antagonistic action. It is a useful anti-anginal agent that also lowers blood pressure.
The functional hereditary units of BACTERIA.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
Benzopyrroles with the nitrogen at the number one carbon adjacent to the benzyl portion, in contrast to ISOINDOLES which have the nitrogen away from the six-membered ring.
A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.
A benzoic-sulfonamide-furan. It is a diuretic with fast onset and short duration that is used for EDEMA and chronic RENAL INSUFFICIENCY.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
The study of PHYSICAL PHENOMENA and PHYSICAL PROCESSES as applied to living things.
An imidazole derivative with a broad spectrum of antimycotic activity. It inhibits biosynthesis of the sterol ergostol, an important component of fungal CELL MEMBRANES. Its action leads to increased membrane permeability and apparent disruption of enzyme systems bound to the membrane.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
Antibodies produced by a single clone of cells.
Intracellular fluid from the cytoplasm after removal of ORGANELLES and other insoluble cytoplasmic components.
A change from planar to elliptic polarization when an initially plane-polarized light wave traverses an optically active medium. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
The balance of fluid in the BODY FLUID COMPARTMENTS; total BODY WATER; BLOOD VOLUME; EXTRACELLULAR SPACE; INTRACELLULAR SPACE, maintained by processes in the body that regulate the intake and excretion of WATER and ELECTROLYTES, particularly SODIUM and POTASSIUM.
Cyclopentanophenanthrenes with a 5- or 6-membered lactone ring attached at the 17-position and SUGARS attached at the 3-position. Plants they come from have long been used in congestive heart failure. They increase the force of cardiac contraction without significantly affecting other parameters, but are very toxic at larger doses. Their mechanism of action usually involves inhibition of the NA(+)-K(+)-EXCHANGING ATPASE and they are often used in cell biological studies for that purpose.
A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey (C. pygerythrus) is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research.
Inorganic salts of phosphoric acid.
A group of enzymes that are dependent on CYCLIC AMP and catalyze the phosphorylation of SERINE or THREONINE residues on proteins. Included under this category are two cyclic-AMP-dependent protein kinase subtypes, each of which is defined by its subunit composition.
A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.
Substances elaborated by bacteria that have antigenic activity.
The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS.
The physical characteristics and processes of biological systems.
Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations or by parent x offspring matings carried out with certain restrictions. This also includes animals with a long history of closed colony breeding.
A non-essential amino acid that occurs in high levels in its free state in plasma. It is produced from pyruvate by transamination. It is involved in sugar and acid metabolism, increases IMMUNITY, and provides energy for muscle tissue, BRAIN, and the CENTRAL NERVOUS SYSTEM.
An alkaloid derived from the bark of the cinchona tree. It is used as an antimalarial drug, and is the active ingredient in extracts of the cinchona that have been used for that purpose since before 1633. Quinine is also a mild antipyretic and analgesic and has been used in common cold preparations for that purpose. It was used commonly and as a bitter and flavoring agent, and is still useful for the treatment of babesiosis. Quinine is also useful in some muscular disorders, especially nocturnal leg cramps and myotonia congenita, because of its direct effects on muscle membrane and sodium channels. The mechanisms of its antimalarial effects are not well understood.
The quantity of volume or surface area of CELLS.
Na-K-Cl transporter ubiquitously expressed. It plays a key role in salt secretion in epithelial cells and cell volume regulation in nonepithelial cells.
The portion of chromosome material that remains condensed and is transcriptionally inactive during INTERPHASE.
The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.
Negatively charged atoms, radicals or groups of atoms which travel to the anode or positive pole during electrolysis.
An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.
A group of lysosomal proteinases or endopeptidases found in aqueous extracts of a variety of animal tissues. They function optimally within an acidic pH range. The cathepsins occur as a variety of enzyme subtypes including SERINE PROTEASES; ASPARTIC PROTEINASES; and CYSTEINE PROTEASES.
A gene silencing phenomenon whereby specific dsRNAs (RNA, DOUBLE-STRANDED) trigger the degradation of homologous mRNA (RNA, MESSENGER). The specific dsRNAs are processed into SMALL INTERFERING RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA-INDUCED SILENCING COMPLEX. DNA METHYLATION may also be triggered during this process.
The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
... "miR-34a contributes to megakaryocytic differentiation of K562 cells independently of p53". Blood. 114 (10): 2181-92. doi: ... Cell Cycle. 10 (24): 4256-71. doi:10.4161/cc.10.24.18552. PMID 22134354. Yamamura S, Saini S, Majid S, Hirata H, Ueno K, Deng G ... 10.1182/blood-2009-02-205062. PMID 19584398. Welch C, Chen Y, Stallings RL (July 2007). "MicroRNA-34a functions as a potential ... "miR-34a induces the downregulation of both E2F1 and B-Myb oncogenes in leukemic cells". Clinical Cancer Research. 17 (9): 2712- ...
Huang DY, Kuo YY, Chang ZF (2005). "GATA-1 mediates auto-regulation of Gfi-1B transcription in K562 cells". Nucleic Acids Res. ... 2005). "Gfi-1B plays a critical role in terminal differentiation of normal and transformed erythroid progenitor cells". Blood. ... 1998). "The Gfi-1B proto-oncoprotein represses p21WAF1 and inhibits myeloid cell differentiation". Mol. Cell. Biol. 18 (5): ... 2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration". Cell. ...
"MicroRNA-223 reversibly regulates erythroid and megakaryocytic differentiation of K562 cells". J. Cell. Mol. Med. 13 (11-12): ... "Differentiation stage-specific expression of microRNAs in B lymphocytes and diffuse large B-cell lymphomas". Blood. 113 (16): ... Blood. 113 (21): 5237-45. doi:10.1182/blood-2008-11-189407. PMID 19144983. Rainer J, Ploner C, Jesacher S, Ploner A, Eduardoff ... doi:10.1182/blood-2008-10-184077. PMC 2670792. PMID 19047678. Shen J, Ambrosone CB, Zhao H (March 2009). "Novel genetic ...
K562 leukemic cells by Gab2". Blood. 99 (4): 1388-97. doi:10.1182/blood.V99.4.1388. PMID 11830491. Hill RJ, Zozulya S, Lu YL, ... and B-cell antigen receptors". Blood. 93 (6): 1809-16. doi:10.1182/blood.V93.6.1809.406k35_1809_1816. PMID 10068651. "Entrez ... integrin signaling pathway-mediated hematopoietic cell adhesion and migration". Blood. 99 (7): 2351-9. doi:10.1182/blood.V99.7. ... GC-GAP is responsible for the proliferation of astroglioma cells. The interaction between GAB2 and Grb2 at the cell membrane ...
Isolated harmine was found to exhibit a cytotoxic effect on HL60 and K562 leukemic cell lines. This action might explain the ... Louis ED, Zheng W, Jurewicz EC, Watner D, Chen J, Factor-Litvak P, Parides M (December 2002). "Elevation of blood beta- ... Individuals diagnosed with essential tremor have been found to have elevated blood levels of harmala alkaloids. Unlike MAOIs ... previously observed cytotoxic effect of P. harmala on these cancer cells." Harmala alkaloids are considered Schedule 9 ...
"Expression of type II activin receptor genes during differentiation of human K562 cells and cDNA cloning of the human type IIB ... doi:10.1182/blood.V83.8.2163.2163. PMID 8161782. Ishikawa S, Kai M, Murata Y, Tamari M, Daigo Y, Murano T, Ogawa M, Nakamura Y ... Cell Res. 224 (2): 323-34. doi:10.1006/excr.1996.0142. PMID 8612709. Matsuzaki T, Hanai S, Kishi H, Liu Z, Bao Y, Kikuchi A, ... Cell. Biol. 16 (3): 1066-73. doi:10.1128/mcb.16.3.1066. PMC 231089. PMID 8622651. De Winter JP, De Vries CJ, Van Achterberg TA ...
... is typically expressed at low levels in several transformed cell lines-including HeLa, K562, HL-60, and CEM-as well as a ... large range of normal tissues including spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood ... 2002). "A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. ... Proliferating cell nuclear antigen (PCNA) is a protein involved in post-replication MMR. It has been shown that PCNA binds to ...
K562. K562 is highly sensitive to lysis by human NK cells and, over the decades, the K562 51chromium-release assay has become ... CD56dim NK cells are primarily found in the peripheral blood, and are characterized by their cell killing ability. CD56dim NK ... NK cells are thought to be an important cell type in this process. These cells are known as "uterine NK cells" (uNK cells) and ... Natural killer cells (NK cells) and macrophages play a major role in clearance of senescent cells. Natural killer cells ...
Blood, 98 (10): 2887-93, doi:10.1182/blood.V98.10.2887, PMID 11698267 "Cellosaurus K-562 (CVCL_0004)". Cellosaurus. SIB Swiss ... K562 cells were the first human immortalised myelogenous leukemia cell line to be established. K562 cells are of the ... Many factors and components play a role in the cell cycle of K562 cells in terms of growth, cell differentiation, and apoptosis ... abl over-expression may actually increase cell adherence to cell culture plastic. K562 cells can spontaneously develop ...
... is also shown to induce apoptosis in K562 cells, and high doses (120 μM) of zinc result in microglial cell death. One ... One study showed that TPEN induces translocation of cytochrome c from the mitochondria to the cytosol in human peripheral blood ... "Metal chelator TPEN selectively induces apoptosis in K562 cells through reactive oxygen species signaling mechanism: ... In addition to a heavy metal chelator, TPEN is also known to be an inducer of apoptosis., thus it may be toxic to cells. One ...
An enhancer located 3′ of the HBG1 gene is bound by several proteins in PBDEs and K562 cells and is sensitive to DNase I, but ... erythroid cells. All are marked by DNase I hypersensitive sites and footprints, and many are bound by GATA1 in peripheral blood ... Blood. 73 (5): 1081-104. doi:10.1182/blood.V73.5.1081.1081. PMID 2649166. Salzano AM, Carbone V, Pagano L, Buffardi S, De RC, ... Blood. 107 (8): 3359-70. doi:10.1182/blood-2005-07-2888. PMC 1895762. PMID 16380451. Basu P, Morris PE, Haar JL, Wani MA, ...
1991). "Putative tyrosine kinases expressed in K-562 human leukemia cells". Proc. Natl. Acad. Sci. U.S.A. 87 (22): 8913-7. ... 1997). "Thrombopoietin and thrombin induce tyrosine phosphorylation of Vav in human blood platelets". Blood. 89 (8): 2789-98. ... Tyk2 deficiency has more dramatic effects in human cells than in mouse cells. However, in addition to IFN-α and -β and IL-12 ... Blood. 86 (8): 2948-56. doi:10.1182/blood.V86.8.2948.2948. PMID 7579387. Domanski P, Yan H, Witte MM, et al. (1995). " ...
Bruchova-Votavova H, Yoon D, Prchal JT (2010). "miR-451 enhances erythroid differentiation in K562 cells". Leuk Lymphoma. 51 (4 ... Blood. 113 (8): 1794-1804. doi:10.1182/blood-2008-05-155812. PMC 2647672. PMID 18849488. Gal H, Pandi G, Kanner AA, Ram Z, ... "MicroRNA-451 regulates LKB1/AMPK signaling and allows adaptation to metabolic stress in glioma cells". Mol Cell. 37 (5): 620- ... Courts, C; Madea, B (Aug 9, 2011). "Specific Micro-RNA Signatures for the Detection of Saliva and Blood in Forensic Body-fluid ...
"MicroRNA-223 reversibly regulates erythroid and megakaryocytic differentiation of K562 cells". J Cell Mol Med. 13 (11-12): 4551 ... Blood. 115 (9): 1768-78. doi:10.1182/blood-2009-08-240101. PMC 2832809. PMID 20029046. Liu TY, Chen SU, Kuo SH, Cheng AL, Lin ... Cell. 129 (3): 617-31. doi:10.1016/j.cell.2007.02.048. PMID 17482553. Sun G, Li H, Rossi JJ (2010). "Sequence context outside ... Cell. 123 (5): 819-31. doi:10.1016/j.cell.2005.09.023. PMID 16325577. Sugatani T, Hruska KA (2007). "MicroRNA-223 is a key ...
"Expression of coproporphyrinogen oxidase and synthesis of hemoglobin in human erythroleukemia K562 cells". European Journal of ... Blood. 91 (4): 1453-7. doi:10.1182/blood.V91.4.1453. PMID 9454777. Susa S, Daimon M, Kondo H, Kondo M, Yamatani K, Sasaki H ( ...
2002). "Mitochondrial ferritin: a new player in iron metabolism". Blood Cells Mol. Dis. 29 (3): 376-83. doi:10.1006/bcmd. ... an antioxidant-responsive element of the human ferritin H gene and role of Ref-1 during erythroid differentiation of K562 cells ... 2003). "Mitochondrial ferritin expression in erythroid cells from patients with sideroblastic anemia". Blood. 101 (5): 1996- ... Cell Biol. 40 (10): 2110-9. doi:10.1016/j.biocel.2008.02.010. hdl:10447/35800. PMID 18372207. Snyder AM, Wang X, Patton SM, et ...
In certain doses, AuNSs that have positively-charged ligands are toxic in monkey kidney cells (Cos-1), human red blood cells, ... Large AuNPs conjugated with biotin, cysteine, citrate, and glucose were not toxic in human leukemia cells (K562) for ... cell health, cell stress, how many cells are taken into a cell), and the capping ligands in solution. In vivo assessments can ... AuNSs size 1.4 nm were found to be toxic in human skin cancer cells (SK-Mel-28), human cervical cancer cells (HeLa), mouse ...
"Duffy Antigen Receptor for Chemokines Mediates trans-Infection of HIV-1 from Red Blood Cells to Target Cells and Affects HIV- ... Chaudhuri A, Zbrzezna V, Polyakova J, Pogo AO, Hesselgesser J, Horuk R (1994). "Expression of the Duffy antigen in K562 cells. ... The Duffy antigen is located on the surface of red blood cells, and is named after the patient in whom it was discovered. The ... Indeed, nucleated red blood cells present in the bone marrow have high expression of DARC, which facilitates their direct ...
K562. K562 is highly sensitive to lysis by human NK cells and, over the decades, the K562 51chromium-release assay has become ... Instead, investigators are working on using allogeneic cells from peripheral blood, which requires that all T cells be removed ... NK cells are thought to be an important cell type in this process.[17] These cells are known as "uterine NK cells" (uNK cells) ... NKT cell activity promotes NK cell activity by secreting interferon gamma. In contrast to NKT cells, NK cells do not express T- ...
... synthetase and active transport of glutathione S-conjugate are responsive to heat shock in K562 erythroid cells". The Journal ... Blood. 75 (1): 271-3. doi:10.1182/blood.V75.1.271.271. PMID 2294991. Konrad PN, Richards F, Valentine WN, Paglia DE (Mar 1972 ... Collins FS, Rossant J, Wurst W (Jan 2007). "A mouse for all reasons". Cell. 128 (1): 9-13. doi:10.1016/j.cell.2006.12.018. PMID ... Blood. 94 (8): 2890-4. doi:10.1182/blood.V94.8.2890.420k16_2890_2894. PMID 10515893. Ristoff E, Augustson C, Geissler J, de ...
... and in the capillary endothelial cells composing the blood-brain barrier and blood-testis barrier, where it pumps them back ... "Rab4 interacts with the human P-glycoprotein and modulates its surface expression in multidrug resistant K562 cells". ... P-gp is also used to differentiate transitional B cells from naive B cells. Dyes such as rhodamine 123 and MitoTracker dyes ... in liver cells where it pumps them into bile ducts, in the cells of the proximal tubule of the kidney where it pumps them into ...
"The chronic myelocytic cell line K562 contains a breakpoint in bcr and produces a chimeric bcr/c-abl transcript". Mol. Cell. ... Blood. 81 (6): 1567-72. doi:10.1182/blood.V81.6.1567.1567. PMID 8453102. Mitchell P, Petfalski E, Tollervey D (1996). "The 3' ... Blood. 78 (4): 1078-84. doi:10.1182/blood.V78.4.1078.1078. PMID 1868241. Zhu QS, Heisterkamp N, Groffen J (1990). " ... Cell. Biol. 7 (9): 3231-6. doi:10.1128/mcb.7.9.3231. PMC 367959. PMID 3313010. Groffen J, Heisterkamp N, Grosveld F, et al. ( ...
... and in the capillary endothelial cells composing the blood-brain barrier and blood-testis barrier, where it pumps them back ... "Rab4 interacts with the human P-glycoprotein and modulates its surface expression in multidrug resistant K562 cells". ... cell surface. • extracellular exosome. • apical plasma membrane. Biological process. • stem cell proliferation. • xenobiotic ... P-gp is also used to differentiate transitional B cells from naive B cells. Dyes such as rhodamine 123 and MitoTracker dyes ...
... and RXR beta like proteins in interferon induced HLA class I and beta globin gene transcription in K562 erythroleukaemia cells ... He also delivered two lectures of the Indian Society of Hematology and Blood Transfusion; J. B. Chatterjee Memorial Oration ( ... Osteoarthritis Ginseng Commiphora wightii Blood disorders India portal Medicine portal Long link - please select award year to ...
ScaBER urinary bladder cancer cells, and K562 erythroleukemia and HL-60 promyelocyte leukemic blood cancer cells caused an ... Tca-8113 cells, HeLa uterine cervix cell lines, A549 cells, MDA-MB-435 breast cells, and HepG2 cells but they had no ... lung cancer A549 cells and NCL-H446 cells, HepG2 liver cancer cells, LS-174 colon cancer cells, SiHa uterine cervix cancer ... blood clot) formation; 4) promote fibrinolysis thereby dissolving blood clots; and 5) inhibit vascular smooth muscle cell ...
GPR31 mRNA is expressed at low levels in several human cell lines including K562 cells (human myelogenous leukemia cell line), ... the extravasation and local accumulation of circulating blood neutrophils and mononuclear cells. These results suggest these ... T lymphocye cell line), Hut78 cells (T cell lymphoma cell line), HEK 293 cells (primary embryonic kidney cell line), MCF7 cells ... Further studies in animal models suggest that the 12S-HETE made by pancreatic beta cells (or possibly alpha cells or other cell ...
"Harmine showed cytotoxicity against HL60 and K562 cell lines. This could explain the cytotoxic effect of Peganum harmala on ... Journal of Cerebral Blood Flow & Metabolism. 26 (3): 330-344. doi:10.1038/sj.jcbfm.9600197. PMID 16079787. Foley, Paul Bernard ... These islet sub-cells are normally very resistant to growth stimulation in the adult stage of a human's life, as the cell mass ... Harmine has been shown to promote differentiation of osteoblasts (bone-forming cells), and chondrocytes (cells in the cartilage ...
Matrine upregulates the cell cycle protein E2F-1 and triggers apoptosis via the mitochondrial pathway in K562 cells. Eur J ... Furthermore, oxymatrine has been shown to decrease cardiac ischemia (decreased blood perfusion), myocardial injury, arrhythmias ... Anticancer effects of the Chinese medicine matrine on murine hepatocellular carcinoma cells. Planta Med 74:245-251 Jiang H, Hou ...
... or K562, the basophilic leukemia cell line KU812, nor on cells such as HL60 or EoL-3 that have been differentiated towards an ... Blood. 101 (12): 5014-20. doi:10.1182/blood-2002-10-3058. PMID 12609831. Cherry WB, Yoon J, Bartemes KR, Iijima K, Kita H (June ... Only low levels are detected on the human mast cell sub-line HMC-1.1; however, the HMC-1.2 cell line, which bears a second KIT ... IL-5 stimulation also appears to alter the mode of cell death of eosinophils induced by Siglec-8 ligation in that cell death ...
Instruments that separate cancer cells from healthy cells have been made.[18] Platelets have been separated from whole blood ... Chin, S., et al., Rapid assessment of early biophysical changes in K562 cells during apoptosis determined using ... Strains of bacteria and viruses[26][27] red and white blood and cells.[citation needed] DEP can also be used to detect ... DEP has made it possible to characterize and manipulate biological particles like blood cells, stem cells, neurons, pancreatic ...
"Tyrphostin-induced inhibition of p210bcr-abl tyrosine kinase activity induces K562 to differentiate". Blood. 82 (12): 3524-9. ... Yaish P, Gazit A, Gilon C, Levitzki A (1988). "Blocking of EGF-dependent cell proliferation by EGF receptor kinase inhibitors ... "Effects of a selective inhibitor of the Abl tyrosine kinase on the growth of Bcr-Abl positive cells". Nat Med. 2 (5): 561-6. ...
"Putative tyrosine kinases expressed in K-562 human leukemia cells". Proceedings of the National Academy of Sciences of the ... Blood. 84 (6): 1931-41. doi:10.1182/blood.V84.6.1931.1931. PMID 7521695. Shieh YS, Lai CY, Kao YR, Shiah SG, Chu YW, Lee HS, Wu ... AXL is expressed on the tumor cells as well as adjacent immune cells including dendritic cells, macrophages, and NK cells. Axl ... "Expression Analysis Highlights AXL as a Candidate Zika Virus Entry Receptor in Neural Stem Cells". Cell Stem Cell. 18 (5): 591- ...
"Antitumor effect of non-steroid glucocorticoid receptor ligand CpdA on leukemia cell lines CEM and K562" (PDF). Biochemistry. ... and metabolic effects like weight gain or increase of blood glucose were practically inexistent. Both non-selective ... Cell. 93 (4): 531-41. doi:10.1016/S0092-8674(00)81183-6. PMID 9604929. S2CID 6524157. Reichardt HM, Tronche F, Bauer A, Schütz ... "Novel steroid receptor phyto-modulator compound a inhibits growth and survival of prostate cancer cells". Cancer Research. 68 ( ...
... anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This gene is thought to ... 1994). "Glycoinositol phospholipid anchor-defective K562 mutants with biochemical lesions distinct from those in Thy-1- murine ...
"Tyrphostin-induced inhibition of p210bcr-abl tyrosine kinase activity induces K562 to differentiate". Blood. 82 (12): 3524-9. ... "Effects of a selective inhibitor of the Abl tyrosine kinase on the growth of Bcr-Abl positive cells". Nat Med. 2 (5): 561-6. ... doi:10.1182/blood.V82.12.3524.3524. PMID 7505115. Meydan N, Grunberger T, Dadi H, Shahar M, Arpaia E, Lapidot Z, Leeder JS, ... "Blocking of EGF-dependent cell proliferation by EGF receptor kinase inhibitors". Science. 242 (4880): 933-935. Bibcode:1988Sci ...
"VDAC2 and aldolase A identified as membrane proteins of K562 cells with increased expression under iron deprivation". Molecular ... which is vital to maintain NO production for proper blood circulation to the lungs. As a result, VDAC2 is significantly ... Danial NN, Korsmeyer SJ (Jan 2004). "Cell death: critical control points". Cell. 116 (2): 205-19. doi:10.1016/S0092-8674(04) ... Cell. 127 (3): 635-48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573. Yoo BC, Fountoulakis M, Cairns N, Lubec G ...
... growth and glycosaminoglycan synthesis of endothelial cells through its action on smooth muscle cells in an artificial blood ... verapamil to P-glycoprotein on specific sites and transport of verapamil outward across the plasma membrane of K562/ADM cells ... cells. Doxorubicin works by putting rampant cancer cells into a senescent state. MK, in WT-MK transfected cells versus WT cells ... non-resistant cell type, the gene for MK was transfected into WT cells to determine if MK was overexpressed in the WT cells ...
... upregulates miR-663 in human THP-1 monocytic cells, human blood monocytes and MCF7 brest cancer cells. Endogenous activator ... "Effect of CpG island methylation on microRNA expression in the k-562 cell line". Biochemical Genetics. 50 (1-2): 122-34. doi: ... miR-663 introduction into the two human GC cell lines BGC823 and SNU5 induces morphology changes and suppresses cell ... Jian P, Li ZW, Fang TY, Jian W, Zhuan Z, Mei LX, Yan WS, Jian N (April 2011). "Retinoic acid induces HL-60 cell differentiation ...
K562 cells, SEA or both SEA and K562 cells were cocultured with mononuclear cells (MNCs) from four normal human cord blood for ... both of SEA and K562 cell group respectively. The expressed level of CD3(epsilon) chain in both SEA and K562 group was ... RESULTS The expressed level of CD3(epsilon) chain on MNCs was slightly decreased in K562 cell group and were increased in the ... which were stimulated by K562 cells. METHODS The anti-CD3 antibodies(mAb), ...
... cell lines and compared with the normal PBMCs (peripheral blood mononuclear cells). Both leukemic K-562 and THP-1 cells showed ... Cell number estimation and cell cycle analysis. The cells were plated in 60-mm culture dishes (0.15 × 106 cells/4 ml/PD) kept ... K-562 and THP-1 cells. (C) HK-II enzyme kinetics was performed in K-562 and THP-1 cells. Data presented here are NADP to NADPH ... K-562 and THP-1 cells. (C) HK-II enzyme kinetics was performed in K-562 and THP-1 cells. Data presented here are NADP to NADPH ...
... k562 cells include Flow Cytometric Analysis of Natural Killer Cell Lytic Activity in Human Whole Blood, Methods to Evaluate ... and Induced Pluripotent Stem Cells (iPSCs), Preparation and Use of HIV-1 Infected Primary CD4+ T-Cells as Target Cells in ... Sleeping Beauty and Artificial Antigen Presenting Cells to Genetically Modify T Cells from Peripheral and Umbilical Cord Blood ... and Functional Assessment of Human Peripheral Blood NK Cells , Evaluation of the Spatial Distribution of γH2AX following ...
Bortezomib is a reversible inhibitor of the 26S proteasome in-ducing cell cycle arrest in G2/M phase, apop-tosis by inhibition ... of the imatinib/bortezomib combination and the responsible apoptotic mechanisms in-duced by this combination in K562 cells. The ... through increase in caspase-3 enzyme activity and decrease in mitochondrial membrane potential in human K562 CML cells. ... Blood, 110, 469-470. [26] Yong, A.S.M., Keyvanfar, K., Hensel, N., et al. (2008) Primitive quiescent CD34+ cells in chronic ...
Inhibition of the ABL kinase activity blocks the proliferation of BCR/ABL+ leukemic cells and induces apoptosis. Blood Cells ... Effects of PD180970, PP1, and PP2 on cell growth and viability of K562 and HL60 cells. K562 or HL60 cells (1.5× 105/ml) in ... A and B, effects of PD180970 on K562 and HL60 cells. C and D, effects of PP1 and PP2 on K562 and HL60 cells. DM, DMSO; PD, ... K562 cells were treated with or without PD180970 (0.5 μm) for the indicated times. A, cytospins of K562 cells were examined by ...
C, K562-FOXO3a(A3):ER and K562-ER cells as well as the parental K562 cells were transiently transfected with 1 μg of the human ... 3B). Similarly, 4-OHT also triggered ABCB1 promoter activity in K562-FOXO3a:ER cells but not in the parental K562 cells or K562 ... K562-FOXO3a(A3):ER and K562-ER cells were treated with 4-OHT for the indicated times. A, cell lysates were prepared at the ... C, ABCB1 mRNA levels of the K562 cells were analyzed by RTQ-PCR as described. D, the K562 cells were transiently transfected ...
Background As a disease of hematopoietic stem cell, chronic myeloid leukemia (CML) possesses unique biological and clinical ... Blood. 2010;116:772-82.CrossRefPubMedGoogle Scholar. *. 9.. Kiss-Toth E, Juhaszne Szalai A, Koska P, Szebeni J, Kiss-Toth E, ... we carried out CDA gene silencing in K562 cells. Upon CDA gene silencing, the cell proliferation was inhibited and the cells ... Mechanism of CDA in the proliferation, apoptosis and cell cycle of K562 cells. a Protein bands of PI3K, p-AKT/t-AKT, p-ERK1/2/t ...
Previous analysis of the hemoglobin phenotype of the K562 human erythroleukemia cell line showed regulated expression of the ... B Mookerjee, MO Arcasoy, GF Atweh; Spontaneous delta- to beta-globin switching in K562 human leukemia cells. Blood 1992; 79 (3 ... Spontaneous delta- to beta-globin switching in K562 human leukemia cells B Mookerjee B Mookerjee ... In this report, we describe the isolation of a variant of the K562 cell line that actively expresses beta-globin messenger RNA ...
The expression of major carbohydrate antigens carried by polylactosaminyl chains in human erythroleukemia cell lines, K562 and ... K562 cells were i+H- X+, with a minor population of I+ cells. HEL cells were I-i+H+X-. The presence of the I+ population in ... cells were sorted by FACS fluorometer. HEL cells and these K562 clones provide a useful experimental model for studying the ... Carbohydrate antigen profiles of human erythroleukemia cell lines HEL and K562 R Kannagi , R Kannagi ...
In this study the effect of RJ on peripheral blood mononuclear cells cytotoxicity against the K562 Erythroleukemia cell line ... Effect of Royal Jelly on Peripheral Blood Mononuclear Cells Cytotoxicity against the K562 Erythroleukemia Cell Line ... Effect of Royal Jelly on Peripheral Blood Mononuclear Cells Cytotoxicity against the K562 Erythroleukemia Cell Line. Journal of ... The cells were then incubated with different concentrations of RJ (5 mg/ml, 10 mg/ml, 25 mg/ml) for 72 hours. Subsequently, ...
1-3. Lane 13, K562 cells. Lane 14, cord blood CD34+ cells. Normal mouse bone marrow was used as a control. ... The role of blood stem cells in hematopoietic cell renewal. Stem Cells. 1998. 16:13-29. View this article via: PubMed Google ... Live cells were gated in R1, and then CD34 cells were gated against lineage cells. R2 represents the CD34+Lin- gate. Cells in ... cells were selected by FACS. Significantly more CD34+CD38-Lin- cells were detected among mononuclear cells from AC-SCD blood ...
... tissue culture cells, plant tissue and Gram-positive and Gram-negative bacteria. DNA purified with this system is suited for ... HMW DNA Extraction Kit is designed for isolating high-molecular-weight DNA from white blood cells , ... Samples of human blood and cultured K562 mammalian cellswere used to extract high-molecular-weight DNA using the Wizard® HMW ... including whole blood, plant leaf, tissue culture cells and Gram-positive and Gram-negative bacteria. With a workflow that can ...
Knockdown of FOXO3 reduces γ-globin expression and protein production in HSPCs and K562 cells. We transduced CD34+ cells ... Induction of red blood cell (RBC) fetal hemoglobin (HbF; α2γ2) ameliorates the pathophysiology of sickle cell disease (SCD) by ... FoxO3 regulates neural stem cell homeostasis. Cell Stem Cell. 2009;5(5):527-539. ... on HbF-immunostaining cells (F cells), as measured by FACS. Both metformin and HU increased the percentage of F cells; the 2 ...
Flow Cyt: Use 10µl for 106 cells, or 100µl whole blood. Not yet tested in other applications. Optimal dilutions/concentrations ... Flow cytometric analysis of human peripheral blood granulocytes with 10µl for 106 cells of anti human CD32 FITC conjugated ( ... Cell Biology. Epigenetics. Metabolism. Developmental Biology. By research area. Immunology. Microbiology. Neuroscience. Signal ... Cell and tissue imaging tools. Cellular and biochemical assays. By product type. Proteins and Peptides. Proteomics tools. ...
2 and gene-modified K562 feeder cells that expressed membrane-bound IL-21 (K562-mbIL21), which led to a generation of ... The second model, in which NK cells were restimulated weekly with IL-2 alone and once on the sixth week with K562-mbIL21 and IL ... Obtaining the progeny of a single cell by cloning the original population is one of the ways to study NK cell heterogeneity. In ... The first model, which included weekly restimulation of clones with K562-mbIL21 and IL-2, resulted in the generation of ...
doi: 10.1182/blood-2008-09-178707. Epub 2008 Dec 18. Research Support, N.I.H., Extramural; Research Support, Non-U.S. Govt ... Identification of Itch as a target of miR106b in K562 cells. (A) Complementary binding between miR106b and the 3′UTR of Itch. ( ... Identification of Itch as a target of miR106b in primary leukemia cells. (A) Primary leukemia cells were transfected with 300 ... K562 cells were transfected with short interfering RNA that were scrambled or against Itch for 48 hours, followed by ...
Cytokines / blood * Drugs, Chinese Herbal / pharmacology* * Female * Humans * K562 Cells * Killer Cells, Natural / drug effects ...
BCL11A represses HBG transcription in K562 cells. Blood Cells Mol Dis 2009;42(2):144-149. ... occasional red blood cell transfusions). The only patient classified as having thalassemia major but bearing 4 positive alleles ... A QTL influencing F cell production maps to a gene encoding a zinc-finger protein on chromosome 2p15. Nat Genet 2007;39(10): ... Your Name) has forwarded a page to you from Blood Journal Message Body (Your Name) thought you would be interested in this ...
... has been shown to inhibit cell growth in cancer cells. Tehranolide was purified from Artemisia diffusa. To detect cell ... c Intracellular levels of cAMP in artemisinin-treated K562 cells. d PKA activity in tehranolide-treated K562 cells. PKA ... Human peripheral blood was obtained from healthy volunteers (aged 25-50 years). Blood samples were obtained by venipuncture of ... b Intracellular levels of cAMP in tehranolide-treated K562 cells. K562 cells were treated with varying concentrations of ...
Flow Cytomtry analysis of K562 cells labeling Iba1 with ab48004 at 10µg/mL (blue). Cells were fixed with paraformaldehyde and ... Lane 1 : Human peripheral blood mononucleocyte lysate in RIPA buffer. Lane 2 : Human peripheral blood mononucleocyte lysate in ... Cell lines and Lysates. Multiplex Assays. By research area. Cancer. Cardiovascular. Cell Biology. Epigenetics. Metabolism. ... Immunocytochemistry/ Immunofluorescence analysis of Caco-2 cells labeling Iba1 with ab48004 at 10µg/mL. Cells were fixed with ...
... "miR-34a contributes to megakaryocytic differentiation of K562 cells independently of p53". Blood. 114 (10): 2181-92. doi: ... Cell Cycle. 10 (24): 4256-71. doi:10.4161/cc.10.24.18552. PMID 22134354. Yamamura S, Saini S, Majid S, Hirata H, Ueno K, Deng G ... 10.1182/blood-2009-02-205062. PMID 19584398. Welch C, Chen Y, Stallings RL (July 2007). "MicroRNA-34a functions as a potential ... "miR-34a induces the downregulation of both E2F1 and B-Myb oncogenes in leukemic cells". Clinical Cancer Research. 17 (9): 2712- ...
Huang DY, Kuo YY, Chang ZF (2005). "GATA-1 mediates auto-regulation of Gfi-1B transcription in K562 cells". Nucleic Acids Res. ... 2005). "Gfi-1B plays a critical role in terminal differentiation of normal and transformed erythroid progenitor cells". Blood. ... 1998). "The Gfi-1B proto-oncoprotein represses p21WAF1 and inhibits myeloid cell differentiation". Mol. Cell. Biol. 18 (5): ... 2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration". Cell. ...
... and tested the in vitro cytotoxic activity of CIK cells against NK-sensitive, chronic myeloid leukaemia K562 cells. CIK cells ... Peripheral blood mononuclear cells (PBMC) from 10 healthy donors and 4 patients with solid cancer were primed with IFN-γ on day ... released high quantities of IL-12p40 and efficiently lysed K562 target cells. Of interest, the frequency of Treg cells was ... thus manifesting a reduced ability to lyse K562 cells. TG fosters the generation of functional CIK cells with no concomitant ...
2009). BCL11A represses HBG transcription in K562 cells. Blood Cells Mol. Dis. 42, 144-149. ... Cells were plated on poly-lysine-coated slides at 6-8×104 cells/ml. Neurons derived from the superficial dorsal horn were ... 1L). Crossing floxed Bcl11a mice to an Ht-PA-Cre mouse line, in which Cre is active in neural crest cells and their derivatives ... 1G-I). In addition, Bcl11a was expressed by the majority of primary sensory neurons in DRG, as well as in a few cells in the ...
2 and tested in a 4-hour 51chromium-release cytotoxicity assay against autologous cultured tumor and myeloid K562 cells. Of 23 ... Peripheral blood lymphocytes derived from patients with renal cell carcinoma were stimulated with interleukin ... A cold target cell inhibition test was performed in 2 patients with unlabeled autologous tumor and K562 cells as cold ... Lysis of autologous tumor cells by peripheral blood lymphocytes treated with interleukin 2 in patients with renal cell ...
The level of lipid peroxidation and the concentration of its products are inversely related to the rate of cell proliferation ... Cytotoxic activity of mononuclear cells (MNC) from peripheral blood of healthy donors was determined by 4 hours Cr51-release ... K562 Cells / drug effects, immunology. Killer Cells, Lymphokine-Activated. Killer Cells, Natural / drug effects, immunology*. ... 34% with Haemin) without affecting cell viability. We further investigated the NK susceptibility of K562 cell line upon in ...
However, AF9 cleavages in cultured normal blood cell were not eliminated by caspase inhibitor. The possible role of CAD and ... The absence of AF9 cleavage in K562 cells further supported the involvement of apoptosis. ... AF9 cleavages in leukaemic cells were abolished by pre-treatment with caspase inhibitor (Z-DEVD-FMK), suggesting the ... In the current study, we show that VP-16 induced cleavage of the AF9 gene in both leukaemic cells and cultured normal blood ...
106 K562 cells or the progeny 2 × 106 K562 cells treated with 1 μM IM for 72 hours. Cells were injected into the left hind ... IL1RAP antibodies block IL-1-induced expansion of candidate CML stem cells and mediate cell killing in xenograft models. Blood ... PTN gene expression in BM cell populations (whole BM, Mac1+/Gr1+ myeloid cells, KSL cells and CD150+CD48/41-KSL, SLAM KSL cells ... Cell lines. The K562 cell line (ATCC, CCL-243) originally derived from the pleural effusion of a human patient with CML (42) ...
... resistant K562/Adr cells, only withaferin A can overcome attenuated caspase activation and apoptosis in K562/Adr cells, whereas ... cell cycle, metastasis, anti-apoptosis and multidrug resistance) in doxorubicin-sensitive K562 and - ... As such, NFκB inhibitors may promote apoptosis in cancer cells and could be used to overcome resistance to chemotherapeutic ... cell survival and P-gp expression and suppresses the apoptotic potential of chemotherapeutic agents. ...
K562 cells were from American Type Culture Collection, whereas Research Blood Components (Boston, MA) supplied blood. DiD, ... and washed twice in T cell media. K562 cells were treated as described in the text. K562 cells were mixed with T cells at an E: ... In WT K562 cells, the TCR Ab was sufficient to induce the T cells to kill the K562 cells. In contrast to the inducible lysis ... K562 cells were rested for 0 (no rest), 6, 12, 24, 48, or 72 h. Once rested, cells were incubated with effector cells for 4 h ...
  • Hexokinase-II (HK-II) is expressed predominantly in cancer cells, which promotes Warburg metabolic phenotype and protects the cancer cells from drug-induced apoptosis. (
  • Taking together, all the results, confirming each other, showed that the combination of the imatinib and bortezomib has considerable synergistic effects on the apoptosis through increase in caspase-3 enzyme activity and decrease in mitochondrial membrane potential in human K562 CML cells. (
  • Kikuchi, S., Nagai, T., Kunitam, M., et al (2007) Active FKHRL1 overcomes imatinib resistance in chronic mye-logenous leukemia-derived cell lines via the production of tumor necrosis factor-related apoptosis-inducing ligand. (
  • Results of nuclear staining, apoptotic-specific poly(ADP-ribose) polymerase cleavage, and annexin V binding assays indicated that PD180970 induced apoptosis of K562 cells. (
  • Further studies showed that PD180970 is an inhibitor of the p210 Bcr-Abl tyrosine kinase and induces apoptosis of K562 cells, whereas it has no detectable effect on Ph-negative HL60 human leukemic cells. (
  • Phosphorylation of FOXO proteins by Akt prevents their nuclear translocation, thereby inhibiting transactivation of target genes [e.g., p27 Kip1 , p130(RB2), cyclin D, Bim , and Bcl-x L ] important for cell proliferation, apoptosis, and differentiation ( 9 ). (
  • Thus, the objective of the present study is to discuss the effect of cytidine deaminase (CDA) gene silencing on the apoptosis and proliferation of CML K562 cells. (
  • Cell proliferation, apoptosis and cell cycle were measured by CCK-8 assay and flow cytometry. (
  • The expression of proteins relevant to cell proliferation, apoptosis and cell cycle was measured by Western blot analysis. (
  • As a result, the proliferation of K562 cells was inhibited after CDA silencing and the cells were mainly arrested in S and G2 phases, while the apoptosis rate of these cells was increased. (
  • Our study demonstrated that CDA gene silencing could inhibit CML cell proliferation and induce cell apoptosis. (
  • Therefore, in this study, we aimed to investigate the effects of CDA gene silencing of the apoptosis and proliferation of CML K562 cells. (
  • Chronic lymphocytic leukemia (CLL) is characterized by cells that exhibit dysfunctional apoptosis. (
  • This event was accompanied by mitochondrial dysfunction, processing of caspase-9, and apoptosis of CLL cells. (
  • As such, NFκB inhibitors may promote apoptosis in cancer cells and could be used to overcome resistance to chemotherapeutic agents. (
  • Although the natural withanolide withaferin A and polyphenol quercetin, show comparable inhibition of NFκB target genes (involved in inflammation, angiogenesis, cell cycle, metastasis, anti-apoptosis and multidrug resistance) in doxorubicin-sensitive K562 and -resistant K562/Adr cells, only withaferin A can overcome attenuated caspase activation and apoptosis in K562/Adr cells, whereas quercetin-dependent caspase activation and apoptosis is delayed only. (
  • This demonstrates that different classes of natural NFκB inhibitors can show different chemosensitizing effects in P-gp overexpressing cancer cells with impaired caspase activation and attenuated apoptosis. (
  • As such, it is believed that inhibitors of NFκB might promote apoptosis in cancer cells and can be helpful to overcome resistance to chemotherapeutic agents. (
  • The absence of AF 9 cleavage in K562 cells further supported the involvement of apoptosis. (
  • Leukemia cells can develop resistance to apoptosis induced by chemotherapeutic agents. (
  • In this study, we generated adriamycin-resistant K562 leukemia (K562/RA) cells and compared the responses of sensitive and resistant leukemia cells to the natural products arsenic trioxide (ATO) and resveratrol (Rsv), with a view to determining whether Rsv potentiates the sensitivity of drug-resistant cells to ATO-induced apoptosis and the associated molecular mechanisms. (
  • Our collective findings indicate that ATO and Rsv synergistically enhance the sensitivity of drug-resistant leukemia cells to apoptosis. (
  • Cell cycle analysis of propidium iodide-stained cells showed that STI-571 significantly reduced PBOX-6-induced G 2 M arrest and polyploid formation with a concomitant increase in apoptosis. (
  • Potentiation of PBOX-6-induced apoptosis by STI-571 was specific to Bcr-Abl-positive leukemia cells with no cytoxic effects observed on normal peripheral blood cells. (
  • Previously, our group identified a novel series of pyrrolo-1,5-benzoxazepine (PBOX) compounds that induce apoptosis in cancer cells derived from both solid tumor types and those from hematological malignancies, suggesting their potential as anticancer agents. (
  • A 60-Hz sinusoidal magnetic field induces apoptosis of prostate cancer cells through reactive oxygen species. (
  • Direct current electrical fields induce apoptosis in oral mucosa cancer cells by NADPH oxidase-derived reactive oxygen species. (
  • Co-cultivation with HZ or treatment with low-micromolar HNE inhibited growth of erythroid cells interfering with cell cycle without apoptosis. (
  • Then, the cell cycle changes were detected by flow cytometric analysis and the early apoptosis of cells was detected by the annexin V/propidium iodide double-staining assay. (
  • On the other hand, hydroquinone induced extensive apoptosis of the cells. (
  • The oncogenic Bcr-Abl tyrosine kinase activates various signaling pathways including phosphoinositide 3-kinase/Akt and nuclear factor-κB that mediate proliferation, transformation, and apoptosis resistance in Bcr-Abl(+) myeloid leukemia cells. (
  • Xanthohumol inhibition of K562 cell viability was associated with induction of apoptosis, increased p21 and p53 expression, and decreased survivin levels. (
  • This receptor is activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL), and thus transduces cell death signal and induces cell apoptosis. (
  • We validated these genomic findings through loss- and gain-of-function studies in normal human CD34 + hematopoietic stem and progenitor cells induced to undergo erythroid differentiation. (
  • The culture conditions described herein should be applicable to cancer-bearing individuals, although the differentiation of fully functional CIK cells may be hindered in patients with advanced malignancies. (
  • The level of lipid peroxidation and the concentration of its products are inversely related to the rate of cell proliferation and directly related to the level of cell differentiation. (
  • It has also been demonstrated that HNE inhibits proliferation and induces differentiation in HL60 cell line. (
  • Constitutive expression of NFκB leads to activation of several factors involved in cell cycle progression and cell differentiation for cancer metastasis. (
  • Our group has identified at least three target sequence areas and one putative splicing factor involved in tissue-specific regulation of red cell isoforms during erythroid differentiation. (
  • Moreover, as cells approach the state of terminal differentiation, there is a clear shift from the intranuclear localization of protein 4.1R to peripheral localization. (
  • Current studies are pursuing the hypothesis that this complex shift in localization and association is indicative of a role for protein 4.1R in signaling terminal differentiation and initiating shutdown of cell proliferation and division. (
  • NK cells, beside exerting cytolytic activity, produce and secrete cytokines, including TNF-α and GM-CSF, that are possibly involved in regulating myeloid and lymphoid precursors maturation/differentiation ( 1 ). (
  • StemSpan™ Megakaryocyte Expansion Supplement (100X) (formerly known as CC220) contains a combination of recombinant human cytokines (SCF, IL-6, IL-9 and TPO) formulated to selectively promote the expansion and differentiation of human megakaryocyte progenitor cells from CD34+ cells isolated from human cord blood (CB) or bone marrow samples. (
  • The defect in vasculogenesis appeared to affect endothelial differentiation, rather than the initial appearance and outgrowth of endothelial cells. (
  • Initial differentiation of yolk sac mesoderm to endothelial cells occurred, but defective differentiation resulted in inadequate capillary tube formation, and weak vessels with reduced cellular adhesiveness. (
  • The data indicate that the primary effect of loss of TGF beta 1 function in vivo is not increased haematopoietic or endothelial cell proliferation, which might have been expected by deletion of a negative growth regulator, but defective haematopoiesis and endothelial differentiation. (
  • An important role for XBP-1, which is a basic-region leucine zipper transcription factor required for plasma cell differentiation and the unfolded protein response ( 8 , 9 ), is underscored by the development of MGUS and MM in XBP-1-transgenic mice ( 10 ). (
  • Differentiation of K562 cells under ELF-EMF applied at different time courses. (
  • The HB-6, CDw75, and CD76 Differentiation Antigens are Unique Cell-Surface Carbohydrate Determinants Generated by the .beta. (
  • White Cell Differentiation Antigens" McMichael, A. J., Oxford University Press. (
  • Cell population heterogeneity poses a major obstacle to understanding complex processes that govern tissue-specific cellular responses, differentiation, and disease development. (
  • The need for scalable single cell analysis is particularly acute in the study of hematopoietic stem cell (HSCs) growth and differentiation. (
  • The analyses of clonal cultures derived from single HSCs have been performed for a number of years and these have already provided some insights into the proliferation kinetics of the input cells, their in vitro responses to varying growth factor conditions, and their rapid loss ex vivo of the differentiation pattern that is typically preserved when they expand in vivo. (
  • These cells lacked erythroid and granulocyte/macrophage potential, but exhibited robust differentiation into the megakaryocyte lineage at a high frequency, both in vivo and in vitro The efficiency and expansion potential of these cells exceeded those of conventional bipotent megakaryocyte/erythrocyte progenitors. (
  • The proteins may also be useful for the induction of growth and/or differentiation of undifferentiated embryonic and stem cells. (
  • Under certain chronic inflammatory conditions, increasing numbers of CD4+ T cells may undergo progressive differentiation, during which cells lose expression of the costimulatory molecules CD28 and CD27 and gain expression of intracellular cytotoxic granules and perforin [11,12]. (
  • In this study the effect of RJ on peripheral blood mononuclear cells cytotoxicity against the K562 Erythroleukemia cell line was investigated. (
  • At the end of the 21-28 day culture period, CD3 + CD56 + cells, derived from CD3 + CD56 - cells, acquire cytotoxicity against various tumour cell targets, including acute myeloid leukaemia (AML), chronic myeloid leukaemia (CML), B and T-cell lymphoma. (
  • Peripheral blood lymphocytes derived from patients with renal cell carcinoma were stimulated with interleukin 2 and tested in a 4-hour 51chromium-release cytotoxicity assay against autologous cultured tumor and myeloid K562 cells. (
  • Since no significant correlation between the degree of cytotoxicity and the pathological findings of tumor stage, grade, cell type or lymphocyte infiltration in the tumor was found, it was impossible to predict from the pathological findings which tumor could be lysed by peripheral blood lymphocytes treated with interleukin 2. (
  • Clonal HLA-A*0201-expressing K562 (K562/A*0201) cells were able to process and present endogenously expressed and exogenously loaded melanoma peptide antigens to HLA-A*0201-restricted cytolytic T lymphocyte clones in cytotoxicity and IFN-gamma ELISPOT assays. (
  • Prompted by advances in the understanding of defects in lymphocyte cytotoxicity, the discovery of adaptive natural killer (NK) cell subsets associated with certain viral infections, and compelling links between stress, adrenaline, and cytotoxic lymphocyte function, we reassessed the role of cytotoxic lymphocytes in ME/CFS. (
  • Furthermore, the NKp30, among natural cytotoxicity receptors, appeared to be primarily involved in triggering NK cells upon interaction with BMSC. (
  • indeed, the natural cytotoxicity receptors (NCRs) ( 14 ), NKp30 and NKp46, have been shown to have a key role in inducing the killing of autologous dendritic cells (DC) ( 12 ). (
  • Other surface receptors such as the activation-dependent markers CD25, CD69, HLA-DR, and typical NK cell receptors like the natural cytotoxicity receptors (NCR), NKG2D, and CD161 are equally expressed on both subsets. (
  • Our data indicated that matrine had potent reversal properties augmenting cytotoxicity of cancer medicines on K562/ADR cells as well as apoptotic rates induced by doxorubicin. (
  • Viral vectors have been used to genetically modify in vitro expanded NK cells to express chimeric antigen receptors (CARs), which confer cytotoxicity against tumors. (
  • Acquisition of anti-CD19 CARs via trogocytosis enhanced NK cell-mediated cytotoxicity against the B-cell acute lymphoblastic leukemia (B-ALL) cell lines and primary B-ALL cells derived from patients. (
  • To our knowledge, this is the first report that describes the increased cytotoxicity of NK cells following the acquisition of CARs via trogocytosis. (
  • We have previously genetically modified in vitro expanded NK cells to express DAP10 and the chimeric NKG2D receptor containing the CD3ζ signal domain, which altered the balance between the activating and inhibitory signals of NK cells and enhanced the cytotoxicity against NKG2D ligand-bearing tumors [3] . (
  • Further, expression of anti-CD19 chimeric antigen receptors (CARs) containing 41BB and CD3ζ signal domains on NK cells enhanced the activating signals originating from CD19 antigen engagement, leading to cytotoxicity specifically against B-cell leukemia [4] . (
  • In MGUS bone marrow, CD1d-restricted invariant natural killer (NK) T cells and CD8 + T lymphocytes manifest potent antitumor cytotoxicity ( 13 , 15 ). (
  • Peripheral blood mononuclear cells obtained from cancer patients before rhIL-12 therapy exhibited defective NK cell cytotoxicity and T-cell-proliferative responses. (
  • Here, we show that xanthohumol has in vitro activity against Bcr-Abl(+) cells and clinical samples and retained its cytotoxicity when imatinib mesylate-resistant K562 cells were examined. (
  • Lysis of autologous tumor cells by peripheral blood lymphocytes treated with interleukin 2 in patients with renal cell carcinoma. (
  • From the kinetics study the lysis of autologous tumor cells was detectable on day 2 of interleukin 2 exposure and the peak activity was observed on day 5. (
  • From this observation we concluded that peripheral blood lymphocytes treated with interleukin 2 lysed not only autologous tumor cells but also K562. (
  • However, disease progression may be mediated by other mechanisms that render tumor cells independent of BCR-ABL. (
  • These findings provide convincing evidence that wild-type mice expressing PARP1 cannot compensate for the loss of PARP1 in tumor cells and strengthen the importance of the role of PARP1 as a transcriptional coactivator of HIF-1-dependent gene expression during tumor progression. (
  • HIF signaling has emerged as an important hypoxia-driven response allowing tumor cells to survive, expand, and invade. (
  • and interactions of effector and regulatory cells with each other and with tumor cells. (
  • Human natural killer (NK) cells are part of the innate immune system and play a critical role in the host defense against pathogens and tumor cells by their cytotoxic potential and the production of cytokines and chemokines. (
  • Natural killer (NK) cells have the ability to recognize and eliminate tumor cells, making them ideal candidates for tumor immunotherapy [1] , [2] . (
  • Similarly, T cells captured NKG2D and NKp46 ligands on tumor cells through trogocytosis and promoted NK cell activity [12] . (
  • 4 - 6 Tumor cells xenografted in zebrafish embryos may constitute an alternative to transgenic animals and were used to explore angiogenesis 7 or radiation sensitizers 8 whereas transplantation of tumor cells in transparent adult zebrafish was used to characterize stem cell transplantation 9 and tumor cell extravasation 10 and invasion. (
  • Cytolysis was specific for various tumor cells, but not for normal cells, irrespective of MHC class I expression. (
  • however, tumor cells often use a strategy known as immune evasion [1]. (
  • It is known that the CD8+ T lymphocytes, which have the NK-activating receptor NKG2D on their surface, acquire an NK-like activity and the ability to kill the HLA-negative tumor cells after a prolonged incubation with the IL-15 or IL-2 cytokines [7-9]. (
  • According to our data, a prolonged incubation of lymphocytes with IL-2 leads to an activation of a subpopulation of CD4+CD25+ cells, which is able to kill HLA-negative tumor cells through the FasL-Fas interaction [10]. (
  • To understand the defense mechanisms employed by an organism to guard itself from tumor cells that escape immune control, it is important to consider regulatory protein molecules secreted by cells of the immune system. (
  • We have shown that xanthohumol has antiangiogenic properties in vitro and in vivo where it inhibited proliferation of endothelial and Kaposi's sarcoma-derived tumor cells in vitro , prevented angiogenesis in the Matrigel sponge model, and reduced Kaposi's sarcoma xenograft growth in vivo . (
  • Moreover, targeting both tumor cells and endothelial cells with agents possessing cytotoxic and antiangiogenic activities may lead to synergistic antitumor effects interrupting a reciprocal stimulatory loop between leukemia and endothelial cells. (
  • Arm II: Patients receive GM-K562 cell vaccine intradermally once every 3 weeks for a minimum of 6 months. (
  • Patients receive GM-K562 cell vaccine subcutaneously once in weeks 0, 3, 6, 9, and 17 in the absence of disease progression or unacceptable toxicity. (
  • TG-driven CIK cells expressed a constellation of NK activating/inhibitory receptors, such as CD158a and CD158b, NKp46, NKG2D and NKG2A/CD94, released high quantities of IL-12p40 and efficiently lysed K562 target cells. (
  • Cytokine-induced killer (CIK) cells are typically differentiated in vitro with interferon (IFN)-γ and αCD3 monoclonal antibodies (mAb), followed by the repeated provision of interleukin (IL)-2. (
  • We also quantified the frequency of bona fide regulatory T cells (Treg), a T-cell subset implicated in the down-regulation of anti-tumor immunity, and tested the in vitro cytotoxic activity of CIK cells against NK-sensitive, chronic myeloid leukaemia K562 cells. (
  • We further investigated the NK susceptibility of K562 cell line upon in vitro treatment with HNE. (
  • 2'-2'-difluorodeoxycytidine: in vitro effects on cell-mediated immune response. (
  • The in vitro action of interferon (IFN)-α and IFN-γ from six healthy donors and ten patients with multiple sclerosis (MS) on natural killer (INK) activity of peripheral blood lymphocytes (PBL) was studied in an autologous system. (
  • Autologous IFN-α and IFN-γ did not augment NK activity of PBL from healthy donors in vitro , whereas in samples from MS patients the IFNs strongly stimulated NK cell cytotoxic function. (
  • In this study we have analyzed the interaction between in vitro cultured bone marrow stromal cells (BMSC) and NK cells. (
  • Because lipophilic cell tracking dyes such as PKH26, PKH67, and CellVue ® Claret can be used to label almost any cell, they have enabled cancer biologists to track a wide variety of tumor and immune cell functions in vitro and in vivo . (
  • In vitro studies with peripheral blood mononuclear cells of healthy donors showed that this modulation of CD16, CD6, CD69, and HLA-DR could also be induced experimentally. (
  • We demonstrate that eIF4G interacts with CBC in vitro and that, in addition to its cytoplasmic localization, there is a significant nuclear pool of eIF4G in mammalian cells in vivo. (
  • The Anticoagulant Effects of the Hookworm, Ancylostoma ceylanicum: Observations on Human and Dog Blood In Vitro and Infected Dogs in Vivo," Thromb Haemostas, 51:222-227 (1984). (
  • Effects of In Vivo Prednisone on In Vitro Eosinophil and Neutrophil Adherence and Chemotaxis", Blood 53:633-641 (1979). (
  • 1992 ) Differential modulation of vascular cell integrin and extracellular matrix expression in vitro by TGF-beta1 correlates with reciprocal effects on cell migration. (
  • The majority of obtained compounds exert antiproliferativeactivity in vitro toward human: HeLa, Fem-X cells, K562, and LS174 cells, having IC 50 values from 62.20 to 205 μM. (
  • Secretion of the above cytokines and NK-cell method for expanding highly cytotoxic clinical-grade NK cells in vitro cytolytic function were IL-2 dose dependent. (
  • The findings demonstrated, for the first time, that benzene and metabolites induce CYP4F3 in human blood cells both in vivo and in vitro. (
  • ref. 6 ), and B-chronic lymphocytic leukemia cell proliferation in vitro ( 7 ). (
  • 11. An in vitro process for introducing foreign DNA by means of human adenovirus into human cells which have no or only a small number of adenovirus receptors or the adenovirus receptors of which are wholly or partly blocked, characterized in that the cells are brought into contact with a conjugate as described in claim 5. (
  • The combined treatment (CT) of 3-BP and DNR showed synergistic effect on the growth inhibition (GI) of K-562 and THP-1 cells. (
  • Bortezomib is a reversible inhibitor of the 26S proteasome in-ducing cell cycle arrest in G2/M phase, apop-tosis by inhibition of NF-kB. (
  • Here, we show that deacetylase inhibition led to the E2F1- and myc-mediated transcriptional activation of the microRNA miR106b in primary CLL cells. (
  • Additionally, PDE inhibition and consequent cAMP accumulation and PKA activity were required for inhibiting cancer cell growth by tehranolide. (
  • Our results show that tehranolide significantly reduces cell proliferation in a time and dose-dependent manner in K562 cells via CaM inhibition, following PDE inhibition, cAMP accumulation, and consequent PKA activity. (
  • In the present study, the effects of tehranolide on CaM, PDE1, and the cAMP/PKA signaling pathway to define the mechanism of cell growth inhibition were investigated. (
  • A cold target cell inhibition test was performed in 2 patients with unlabeled autologous tumor and K562 cells as cold inhibitors. (
  • In 1 patient unlabeled K562 completely inhibited the tumor lysis but in 1 complete inhibition by autologous tumor and incomplete blockade by K562 were found. (
  • HNE causes a strong inhibition of cells growth (53% vs. 34% with Haemin) without affecting cell viability. (
  • CD27 + /CD45RA − Th1-like effector cells killed K562 target cells through a mechanism that could be enhanced by either compound or TCR Ab and blocked by Src inhibition or butyrophilin 3 isoform A1 (BTN3A1) disruption. (
  • Increased expansions of adaptive NK cells or deviant cytotoxic lymphocyte adrenaline-mediated inhibition were not observed. (
  • NK cells are characterized by the surface expression of receptors for self-HLA class I molecules, including some members of the inhibitory receptor superfamily (IRS), 3 whose engagement leads to the inhibition of cytolysis and cytokine production ( 2 , 3 , 4 ). (
  • MAD1L1 is coiled-coil protein that binds to improperly attached kinetochrore, what results in recruitment and interaction with MAD2L1, activation of the mitotic checkpoint complex, inhibition of the anaphase-promoting complex/cyclosome and cell cycle arrest. (
  • The process by which the leukemic cells prove resistant to TKIs and the restoration of BCR-ABL1 signal transduction from previous inhibition has initiated the pursuit for the causal mechanisms of resistance and strategies by which to surmount resistance to therapeutic intervention. (
  • As ML-ICs and SRCs were both detected in blood of AC-SCD patients only, these assays may both measure primitive progenitors. (
  • ELISPOT assays are increasingly used for a direct detection and quantification of single antigen-specific T cells in freshly isolated peripheral blood mononuclear cells (PBMC). (
  • However, one major limitation for the broad clinical implementation of ELISPOT assays is the lack of an inexhaustible source of suitable HLA-matched antigen-presenting cells (APC). (
  • In search of alternative APC for ELISPOT assays, the human chronic myelogenous leukemia cell line K562 that per se does not express HLA class I and class II molecules on the cell surface was transfected with the HLA-A*0201 gene. (
  • K562/A*0201 cells were then used as APC in IFN-gamma spot assays to detect ex vivo CD8(+) T lymphocytes responsive to known HLA-A*0201-binding peptide epitopes derived from cytomegalovirus, Epstein-Barr virus, influenza virus and melanoma in PBMC from HLA-A*0201-positive donors. (
  • Patients who achieve a molecular complete remission (CR) (defined as BCR-ABL-negative disease confirmed by 2 PCR assays separated by 1 month) at the end of the 6-month period, discontinue study therapy and are monitored for disease recurrence by blood tests every 4 weeks. (
  • Samples are analyzed by ELISPOT assays on peripheral blood mononuclear cells, for HLA typing and HLA-A2 expression by the standard NIH microlymphocytotoxicity test, for peptides by ELISPOT assays, and for immunologic response by other exploratory assays. (
  • Additionally, adhesion assays to VCAM-1 were performed using K562 α4 transfectant cells. (
  • The effects of CD44 on proliferation of K562 cells were determined using the MTT and colony formation assays, and even in a nude mouse transplantation model. (
  • Baran, Y., Salas, A., Senkal, C., et al (2007) Alterations of ceramide/sphingosine 1-phosphate rheostat involved in the regulation of resistance to imatinib-induced apop-tosis in K562 human chronic myeloid leukemia cells. (
  • Kwee, J.K., Luque, D.G., dos Santos Ferreira, A.C., et al (2008) Modulation of reactive oxygen species by anti-oxidants in chronic myeloid leukemia cells enhances imatinib sensitivity through survivin downregulation. (
  • A) Primary leukemia cells that were normal or del17p were treated with 10 nM LBH589 for 36 hours. (
  • A) Quantitation of Mcm7 and miR106b and in 20 primary leukemia cells exposed to LBH589. (
  • Here we report that chronic myelogenous leukemia cells expressing small interfering RNA against PARP1, which were injected into wild-type mice expressing PARP1, showed tumor growth with increased levels of necrosis, limited vascularization, and reduced expression of GLUT-1. (
  • Olmutinib Reverses Doxorubicin Resistance in ETS1-Overexpressing Leukemia Cells. (
  • CONCLUSIONS Our results suggest that the novel EGFR TKI olmutinib enhances the sensitivity of ETS1-overexpressing leukemia cells to Dox. (
  • CD19 is an ideal target antigen for immunotherapy because it is expressed on nearly all leukemia cells in most patients with B-cell acute lymphoblastic leukemia (ALL) and chronic lymphoblastic leukemia (CLL) [13] , [14] . (
  • Mechanistic analysis revealed that phenol, a major metabolite of benzene , significantly induced the expression of CYP4F3A at both mRNA and protein levels in cultured promyelocytic leukemia cells (HL-60) similarly to all-trans retinoic acid (ATRA). (
  • Based on our reported antiendothelial effects of xanthohumol and on the observation that endothelial and hematopoietic cells are mutually correlated in their development and growth, here we investigated the effects of xanthohumol on Bcr/Abl-expressing leukemia cells. (
  • To overcome these limitations, we demonstrate intelligent frequency-shifted optofluidic time-stretch quantitative phase imaging (OTS-QPI) that acquires bright-field and quantitative phase images of white blood cells (WBCs) containing leukemia cells with high throughput (15,000 cells/s) for deep-learning-based classification. (
  • Adoptive cellular immunotherapy aims at restoring tumour-cell recognition by the immune system, leading to effective tumour cell killing. (
  • A major hurdle to the successful immunotherapy of cancer is represented by the difficulty in generating clinically relevant numbers of immune effector cells with potent in vivo anti-tumour activity, especially in heavily pre-treated patients. (
  • Our results demonstrate that adoptive immunotherapy with peripheral blood lymphocytes activated by interleukin 2 in patients with renal cell carcinoma could be appropriate as a therapeutic procedure. (
  • Natural killer (NK) cells have the capacity to target tumors and are ideal candidates for immunotherapy. (
  • In this study, we used trogocytosis as a non-viral method to modify NK cells for immunotherapy. (
  • An Erythroleukemia cell line derived from a Chronic myeloid leukemia patient in Blast crisis . (
  • Previous analysis of the hemoglobin phenotype of the K562 human erythroleukemia cell line showed regulated expression of the epsilon-, zeta-, gamma-, alpha-, and delta-globin genes. (
  • The expression of major carbohydrate antigens carried by polylactosaminyl chains in human erythroleukemia cell lines, K562 and HEL, was investigated by applying monoclonal antibodies recognizing specific carbohydrate determinants. (
  • Our previous studies have demonstrated that LT can block megakaryocytic polyploidy in TPA (12-O-tetradecanoylphorbol-13-acetate) treated HEL (human erythroleukemia) cell line and found cofilin differentially expressed in TPA and LT pretreatments conditions by 2D-PAGE (two-dimensional polyacrylamide gel electrophoresis) techniqne. (
  • Although earlier engraftment is seen when cells from GCSF-mobilized blood are transplanted than when bone marrow is transplanted, administration of GCSF to patients with SCD can cause significant morbidity. (
  • We tested whether primitive hematopoietic progenitors are spontaneously mobilized in the blood of patients with SCD during acute crisis (AC-SCD patients). (
  • The frequency of myeloid-lymphoid-initiating cells (ML-ICs) and SCID-repopulating cells (SRCs) was significantly higher in blood from AC-SCD patients than in blood from patients with steady-state SCD or from normal donors. (
  • Peripheral blood mononuclear cells (PBMC) from 10 healthy donors and 4 patients with solid cancer were primed with IFN-γ on day 0 and low (50 ng/ml), intermediate (250 ng/ml) and high (500 ng/ml) concentrations of either αCD3 mAb or TG on day 1, and were fed with IL-2 every 3 days for 21 days. (
  • These efforts are based on studies of patients undergoing allogeneic hematopoietic stem cell transplantation (HSCT) for hematologic malignancies. (
  • In patients undergoing HSCT, depleting donor marrow T cells with an antibody to a T cell surface structure results in a dramatic decrease in the incidence of GVHD. (
  • We are exploring the role of regulatory T cells (Tregs) in the development of GVHD and GVL reactions, and have initiated a clinical trial to augment GVL reactivity using an antibody to CTLA4Ig, a molecule that controls immune reactions.We are also investigating vaccination strategies for preventing relapse in allogeneic transplant patients. (
  • Reproducible differences between patients and controls were not found in cytotoxic lymphocyte numbers, cytotoxic granule content, activation status, exocytotic capacity, target cell killing, or cytokine production. (
  • To demonstrate this potential, IM-resistant cells were found in chronic myelogenous leukemia patients with continuous BCR-ABL gene expression but undetectable BCR-ABL protein expression. (
  • In this study, we describe isolation and characterization of cell lines established from CML patients that failed IM therapy. (
  • Therefore, the peripheral NK cell repertoire of patients after kidney transplantation was investigated in order to identify NK cell subsets that may be associated with the individual immune status at the time of their protocol biopsies for histopathological evaluation of the graft. (
  • Here, we could demonstrate that the NK cell repertoire in peripheral blood of kidney transplant patients differs significantly from healthy individuals. (
  • Moreover, in patients, down-modulation of CD16 and CD6 on CD56 dim NK cells was observed with significant differences between Cyclosporin A- and Tac-treated patients. (
  • In summary, we postulate that the NK cell composition in peripheral blood of kidney transplanted patients represents an important hallmark of the efficacy of immunosuppression and may be even informative for the immune status after transplantation in terms of rejection vs. drug-induced allograft tolerance. (
  • Compare clinical response, in terms of 1-year progression-free survival and rate of molecular complete remission, in patients with Philadelphia chromosome-positive chronic myelogenous leukemia (Ph+ CML) in chronic phase who have achieved a complete cytogenetic remission to single-agent imatinib mesylate treated with imatinib mesylate, interferon alfa, and sargramostim (GM-CSF) vs imatinib mesylate and GM-K562 cell vaccine. (
  • Patients who achieve BCR-ABL-negative disease during the additional 6 months of therapy, discontinue study therapy and are monitored for disease recurrence by blood tests every 4 weeks. (
  • If at any time after stopping study therapy blood tests show disease recurrence, patients restart imatinib mesylate and are eligible to cross over to arm II. (
  • Patients with BCR-ABL-negative disease at the end of the 6-month period discontinue study therapy and are monitored for disease recurrence by blood tests every 4 weeks. (
  • Vaccination with autologous myeloblasts admixed with GM-K562 cells in patients with advanced MDS or AML after allogeneic HSCT. (
  • The authors evaluated the appropriateness of antibiotics for the empiric treatment of FN in pediatric patients with cancer in our institution on the basis of changes in the epidemiology of organisms isolated from blood cultures (BCx). (
  • In contrast, MGUS, but not MM, patients generate high-titer anti-MICA antibodies that antagonize the suppressive effects of sMICA and stimulate dendritic cell cross-presentation of malignant plasma cells. (
  • Peripheral blood mononuclear cells obtained after lymphocyte recovery following the administration of a single 500 ng/kg dose of rhIL-12 displayed augmented NK cell cytolytic activity in four of four patients tested and enhanced T-cell proliferation in three of four patients tested. (
  • The expressions of CD44 in different leukemia patients and cell lines were detected by real-time PCR and western blotting. (
  • Firstly, we showed that CD44 expression was increased in several kinds of leukemia patients and K562 cells. (
  • The Philadelphia chromosome in CML gives rise to constitutively active protein tyrosine kinase product BCR-ABL, which is important because in patients with CML, there is clonal expansion of hematopoietic cells that express this fusion gene. (
  • Human leukemic cell lines and blast cells sorted from patients with acute myelogenous leukemia were injected 48 hours post-fertilization and remained in the circulation of zebrafish embryos for several days without affecting their development. (
  • Spliceosome gene mutations found in myelodysplastic syndrome (MDS) patients enhance inflammatory cytokine production in cancer-relevant myeloid cells. (
  • J) IL-6 mRNA was analyzed in lipopolysaccharide (LPS)-induced (20 ng/mL LPS for 4 hours) peripheral blood monocytes isolated from MDS patients either carrying mutations in spliceosome genes (all splice, n=8, 3 SF3B1 , 3 SRSF2 , 2 U2AF1 , color-coded red) or not carrying mutations in these three spliceosome genes (WT, color-coded black). (
  • Determine the safety of GM-K562 cell vaccine in patients with myelodysplastic syndromes. (
  • Wilms' tumor-1 [WT-1], survivin, or proteinase-3), as defined by a 30% increase from baseline in specific cytotoxic T-cells measured by Elispot assay, in patients with myelodysplastic syndromes. (
  • We analyzed differential gene expression in the peripheral white blood cells (WBC) from 7 female patients diagnosed with occupational benzene poisoning and 7 matched controls. (
  • A potential clinical application for the technology developed in this study is in leukemia patients who are treated with hematopoietic (blood cell-forming) cell transplantation. (
  • This lymphocyte population mainly resides in the peripheral blood and bone marrow, although they have also been detected in primary and secondary lymphoid tissues ( 1 , 2 , 3 , 4 , 5 , 6 , 7 ). (
  • Monoclonal gammopathy of undetermined significance (MGUS) is diagnosed in 3% of adults at least 50 years of age and is characterized by a restricted clonal expansion of antibody-secreting plasma cells in the bone marrow ( 1 ). (
  • MGUS evolves at a frequency of 1% per year to full-blown multiple myeloma (MM), a lethal malignancy in which dysregulated plasma cell homeostasis provokes bone marrow compromise, osteolytic lesions, metabolic perturbations, and kidney damage ( 2 , 3 ). (
  • Dendritic cells and proinflammatory factors in the bone marrow may foster plasma cell accumulation ( 11 , 12 ), whereas innate and adaptive lymphocytes may effectuate plasma cell death ( 13 ). (
  • In human bone marrow (BM) the erythroblastic island (EI) is the elementary unit of erythropoiesis in which a central macrophage (MAC) is surrounded by differentiating erythroid cells. (
  • The traditional diagnosis of leukemia relies on pathologists to observe and classify cells on bone marrow smears, which is low-throughput, time-consuming, and subject to human bias. (
  • Those resembling erythema nodosum (EN) show small vessel vasculitis and perivascular lymphocytic and mononuclear cell infiltration and fibrin deposition in the vessel wall, while the punched out ulcers are characterized by a leucocytoclastic vasculitis (neutrophil infiltrate) with fibrinoid necrosis. (
  • Analysis of transfected K562, KD30, and KD225 cells in which FOXO3a activity can be induced by 4-hydroxytamoxifen showed that FOXO3a up-regulates ABCB1 expression at protein, mRNA, and gene promoter levels. (
  • In addition, CDA gene silencing in K562 cells led to down-regulated p-ERK1/2, t-AKT, p-AKT and BCL-2 expression and up-regulated expression of P21, Bax, cleaved caspase-3/total caspase-3 and cleaved PARP/total PARP. (
  • Nevertheless, the underlying mechanism of CDA gene silencing in the biological processes of K562 cells remains to be clarified. (
  • Because significant numbers of ML-ICs and SRCs are mobilized in the blood without exogenous cytokine treatment during acute crisis of SCD, collection of peripheral blood progenitors during crisis may yield a source of autologous HSCs suitable for ex-vivo correction by gene therapy approaches and subsequent transplantation. (
  • Sickle cell disease (SCD) is an inherited hemoglobinopathy that arises from a single-base substitution at codon 6 of the β-globin gene, resulting in the conversion of valine to glutamic acid. (
  • In this work, we sorted single cells into a plate and stimulated them via interleukin (IL)-2 and gene-modified K562 feeder cells that expressed membrane-bound IL-21 (K562-mbIL21), which led to a generation of phenotypically confirmed and functionally active NK cell clones. (
  • In the current study, we show that VP-16 induced cleavage of the AF 9 gene in both leukaemic cells and cultured normal blood cell. (
  • Our laboratory continues to focus on the molecular pathology and physiology of red cell development, the molecular basis of inherited hemolytic anemias, and the use of the red cell homeostatic system as a model to study gene regulation and growth control in other tissues. (
  • Lack of oxygen stabilizes HIF-1, which in turn modulates the gene expression pattern to adapt cells to the hypoxic environment. (
  • Induction of resistance to the Abelson inhibitor STI571 in human leukemic cells through gene amplification. (
  • A genome-wide DNA methylation study performed on CD34+ hematopoietic stem cells from cord blood of five intra-uterine growth restricted (IUGR) neonates and five gestational age and gender-matched controls [ 16 ] identified among others significant methylation differences at the HNF4A gene, which is involved in monogenic diabetes. (
  • In several tumors, we observed myeloid characteristics (myeloperoxidase [MPO] staining) together with rearrangements of the germinal configuration of the T-cell receptor β (TCRβ) gene and/or of the immunoglobulin heavy chain gene, specific for T-cell and B-cell lineages, respectively. (
  • At this stage of illness, T cells specific for SOX-2, a gene product involved in stem cell self-renewal, inhibit the clonogenicity of MM precursors and confer a reduced risk of evolution toward full-blown disease ( 16 ). (
  • SFN-induced gene expression in erythroid K562 and lymphoid cells were compared for each target gene. (
  • A retrovirally induced insertional mutation of the murine Nodal gene results in the absence of mesodermal cell types normally associated with the primitive streak, and is embryonic lethal. (
  • A-I) Cytokine mRNA levels were assayed in K562 cells carrying the indicated spliceosome gene mutations (red) or wild-type (WT) control genes (black). (
  • There is a need for an efficient system of introducing nucleic acid into living cells particularly in gene therapy. (
  • This involves delivering genes into cells in order to achieve, in vivo, the synthesis of therapeutically effective gene products, e.g. in order to replace a defective gene in the event of a genetic defect. (
  • By this is meant viruses in which the gene to be expressed in the cell has been integrated in the genome by recombinant methods. (
  • Measurements of peripheral blood lymphocyte subsets with monoclonal antibodies and argon ion laser flow cytometry resulted in a significant decrease of cells positive for OKT 4 (helper/inducer) and a significant increase of cells positive for OKT 8 (suppressor/cytotoxic) and Leu 7 (natural killer) by interleukin 2 treatment. (
  • K562 cells transfected with HLA-A*0201 or other HLA genes can serve as standard APC for monitoring T lymphocyte responses against tumor and viral peptide antigens. (
  • We validated this strategy in a randomized trial of CD8-depleted donor lymphocyte infusions after T cell-depleted allotransplantation, and are now conducting further trials on CD8 depletion. (
  • Vascular Cell Adhesion Molecule-1 Mediates Lymphocyte Adherence to Cytokine-Activated Cultured human Endothelial Cells," Blood 76:965-970 (1990). (
  • Sequential infusion of donor-derived dendritic cells with donor lymphocyte infusion for relapsed hematologic cancers after allogeneic hematopoietic stem cell transplantation. (
  • Absolute lymphocyte count recovery after allogeneic hematopoietic stem cell transplantation predicts clinical outcome. (
  • rhIL-12-induced lymphopenia involved all major lymphocyte subsets, although natural killer (NK) cell numbers were the most profoundly affected, and CD4 T-cell numbers were the least affected. (
  • Almost all Z-NK cells expressed the lymphocyte-activated marker CD69 and showed dramatically high expression of activation receptors (i.e. (
  • Using the doxorubicin-sensitive K562 cell line and the resistant derivative lines KD30 and KD225 as models, we found that acquisition of multidrug resistance (MDR) is associated with enhanced FOXO3a activity and expression of ABCB1 (MDR1), a plasma membrane P-glycoprotein that functions as an efflux pump for various anticancer agents. (
  • These results show that K562 cells depend to a greater extent on CD44 for proliferation, and CD44 down-regulation may induce a cell cycle arrest through Wnt/β-catenin pathway. (
  • We demonstrate that neutrophils strongly cluster with immature DCs and that activated, not resting, neutrophils induce maturation of DCs that enables these DCs to trigger strong T cell proliferation and T helper type 1 polarization of T cells. (
  • Determine if vaccination with GM-K562 cell vaccine can induce an immune response to common myeloid antigens (e.g. (
  • Tag7 (PGLYRP1) Can Induce an Emergence of the CD3+CD4+CD25+CD127+ Cells with Antitumor Activity. (
  • Comparison of natural killer cell activity against K562 of freshly prepared peripheral blood lymphocytes to that of interleukin 2-treated lymphocytes revealed significant augmentation from 23.6 +/- 9.7 to 65.2 +/- 29.1 per cent. (
  • To assess their in vivo distribution, human peripheral blood lymphocytes (PBLs) and LAK cells were adoptively transferred into nude mice after labeling with indium-111 oxine. (
  • The aim was to evaluate the cyto/genotoxic effects of MEL in human peripheral blood lymphocytes (HPBLs) and the molecular mechanisms involved using a multi-biomarker approach. (
  • Functional studies in human primary erythroid progenitor cells support a role for FOXO3 in γ-globin regulation. (
  • Metformin treatment of human primary erythroid progenitor cells increases fetal hemoglobin in a partially FOXO3-dependent manner. (
  • Altered adhesive interactions with marrow stroma of haematopoietic progenitor cells in chronic myeloid leukaemia. (
  • It contains pre-screened batches of BSA that have been selected to support the optimal growth of human hematopoietic progenitor cells in serum-free media formulations. (
  • It is also suitable for the culture of mouse hematopoietic progenitor cells in serum-free conditions. (
  • We found that LS-TFs from alternate lineages are co-expressed, as proteins, in individual early progenitor cells and quantitative changes of LS-TFs occur gradually rather than abruptly to direct cell-fate decisions. (
  • However, we now know that impaired erythropoietin production and impaired responsiveness of erythroid progenitor cells to this hormone are also important abnormalities contributing to the anemia of chronic disease, and appear to be due to the effects of inflammatory cytokines. (
  • Hildebrand, et al, "Preparation of Nuclear Membranes from Cultured Chinese Hampster Cells by Heparin Treatment", Abstract form, about 1973. (
  • CD22-mediated stimulation of T cells regulates T-cell receptor/CD3-induced signaling," Immunology 89: 10242-10246 (1992). (
  • Subsequently, cytotoxic effect of PBMCs pulsed with RJ on K562 was evaluated by Anexin PI test (Flow cytometry). (
  • Results: In the Anexin PI test, apoptotic property and cytotoxic effect of PBMCs pulsed with RJ on K562 increased significantly compared to those of the control group. (
  • Moreover, tehranolide had a cytotoxic effect on K562 cell line but not on normal human lymphocytes. (
  • However, it has been suggested that Treg lymphocytes can have a direct cytotoxic effect on the cells, releasing perforin and granzymes [15]. (
  • In contrast, PD180970 had no apparent effects on the growth and viability of p210 Bcr-Abl -negative HL60 human leukemic cells. (
  • To detect cell viability and proliferation, MTT assay was performed. (
  • In our experience, a preliminary titration at constant cell concentration is the easiest way to select an optimized dye concentration that gives acceptable post-labeling viability, recovery, and fluorescence intensity as well as unaltered cell function 2,3 . (
  • However, lack of physiologic salts can impair cell viability over prolonged periods, and the dye aggregates will gradually form even in the absence of salts 1-3 . (
  • Evaluation of cell viability, DNA single-strand breaks, and nitric oxide production in LPS-stimulated macrophage RAW264 exposed to a 50-Hz magnetic field. (
  • If cells contain high levels of IPP, as in some cancerous cells, or HMBPP, as in infected cells, they will activate Vγ9Vδ2 T cells to cause a multifaceted immune response ( 3 , 9 , 10 ), including direct target cell lysis. (
  • Natural Killer (NK) cells are the leading maternal immune cells which act as a natural defense system and help in fighting infections. (
  • NK cells activity is propelled by high-stress levels or by an auto-immune disease. (
  • In some situations, such as inflammation in the pelvic cavity, endometriosis, polycystic ovarian syndrome or inflammation in the fallopian tubes, the immune system sends signals to the circulating NK cells in the bloodstream which mistakenly starts attacking the embryo. (
  • NK cells production increases due to an overactive immune system or any inflammation. (
  • While T cell depletion reduces GVHD, its effect on immune reactivity may lead to an increased risk of relapse of certain leukemias after transplantation. (
  • The progress of the disease may be broken down into three phases: an initial phase, characterized by a Type IV immune response (Cell-Mediated Immunity, delayed-hypersensitivity response), a second phase, characterized by a Type III response (Immune Complex), and a third phase where substantial tissue damage occurs. (
  • It is suggested that oral mucosa may be the initial source of antigen and that immune circulating complexes (IC) might be reponsible for extra-oral disease, or that immune circulating complexes may be deposited at a later stage in ulceration, possibly after the initial cell-mediated phase has failed. (
  • Lachmann outlines a scheme which attempts to account for most of the immunological findings in ROU and BS, and the shift from an early, predominantly lymphomonocytic infiltration (probably Type IV, cell- mediated immunity (CMI), delayed hypersensitivity) to a later PMNL infiltration with features associated with a Type III (immune complex) reaction. (
  • Apparently, only activated NK cells lyse self-DC and, in some instances ( 13 ), only immature DC, suggesting that physiologically, this killing occurs during viral infections when NK cells are activated and that this phenomenon may regulate the adaptive immune response. (
  • In the context of kidney transplantation, little is known about the involvement of natural killer (NK) cells in the immune reaction leading to either rejection or immunological tolerance under immunosuppression. (
  • It is generally accepted that naïve CD56 dim NK cells are primarily responsible for cytotoxic activity due to their high content of granules filled with cytotoxins like perforin, granzyme A, and B. In contrast, CD56 bright NK cells are postulated to regulate immune responses primarily through their cytokine production, for example by secretion of interferon (IFN)-γ after stimulation with interleukin (IL)-2, IL-12, or IL-15. (
  • Natural killer cells were recently discussed to be involved in immune recognition after kidney transplantation due to their capability to bind donor-specific anti-HLA-antibodies (donor-specific Ab, DSA) ( Farkash and Colvin, 2012 ). (
  • GM-CSF may help cells that are involved in the body's immune response work better. (
  • Vaccines made from a person's cancer cells may help the body build an effective immune response to kill cancer cells. (
  • To evaluate the feasibility of measuring T-cell responses to Ep-CAM as a potential surrogate target of vaccine-induced immune responses. (
  • Trogocytosis is a process in which membrane patches are exchanged between target and immune cells [5] - [7] . (
  • When an NK cell interacts with a target cell, an immune synapse, which is strong enough to allow the transfer of small membrane patches from one cell to its partner cell, is formed [8] , [9] . (
  • By virtue of its effects on CD4 T cells, CD8 T cells, and NK cells, IL-12 seems to be a key cytokine that regulates cell-mediated immune responses. (
  • We evaluated 206 healthy African-American and Hispanic adolescents, utilizing fresh blood cells and a battery of immune assessments which provides data on enumerative and functional immunological measures. (
  • However, it is unknown whether neutrophils can interact with dendritic cells (DCs) to modulate adaptive immune responses. (
  • Through the release of the chemokines MIP-1α, MIP-3α, and MIP-3β, neutrophils also actively recruit other immune cells like T cells, monocytes, macrophages, and DCs ( 19 , 20 ). (
  • From immune response to targeted cell membrane breakdown, lysates aid in a vast range of resource applications. (
  • These include the NK cells of the innate immune system [3]. (
  • For a long time, the CD4+ T lymphocytes have been considered the only regulatory cells, due to their ability to secrete cytokines that regulate various processes of the immune response. (
  • Cytokines can promote phagocytic activity, generate cytotoxic CD8+ T cells, regulate the production of antibodies and inflammation, and can also suppress the immune response. (
  • Natural killer (NK) immune system cells can be genetically modified to brandish a powerful "on-switch" that prompts them to aggressively attack and kill leukemic cells. (
  • Progress in adapting NK cells to the treatment of ALL had been significantly hampered because researchers were not able to grow large numbers of these immune cells in the laboratory, and because NK cells normally have only weak anti-leukemic activity. (
  • In order to grow large populations of NK cells, the team started with samples of blood containing a variety of different immune system cells. (
  • The technique triggered growth of NK cells specifically, which greatly simplified the ability of the researchers to collect a pure population of these immune cells. (
  • To date, various populations of cytotoxic effector cells have been expanded using robust cell culture procedures and have been administered in a variety of human cancers. (
  • Similar results were obtained using MNC pre-treated with beta-interferon (IFN) as effector cells. (
  • Vγ9Vδ2 effector T cells lyse cells in response to phosphorus-containing small molecules, providing primates a unique route to remove infected or malignant cells. (
  • We examined lysis mediated by human Vγ9Vδ2 effector T cells in response to the naturally occurring ( E )-4-hydroxy-3-methyl-but-2-enyl diphosphate (HMBPP) or a synthetic cell-permeable prodrug, bis (pivaloyloxymethyl) ( E )-4-hydroxy-3-methyl-but-2-enyl phosphonate. (
  • Together, our results show that internalization of HMBPP into target cells is required for BTN3A1-dependent lysis by Vγ9Vδ2 effector T cells. (
  • In addition to the distinct phenotypic features, the two NK cell subsets are also associated with different effector functions. (
  • however, there remain a number of difficulties with respect to the shortage and limited anticancer potency of the effector cells. (
  • NKG2D), interferon-γ, perforin, and granzyme B. Importantly, only 2 hours of reaction at an effector/target ratio of 1:1 was sufficient to kill almost all K562 cells, and the antitumor activity was also replicated in tumor-bearing mice in vivo. (
  • Regulatory CD4+CD25+ T cells, whose function is to suppress effector T lymphocytes, constitute a special Treg group [14]. (
  • It is known that the regulatory CD4+CD25+ subpopulations usually secrete suppressor cytokines that inhibit the activity effector cells. (
  • In unstimulated cells, NFκB is sequestered predominantly in the cytoplasm in an inactive complex through interaction with IκB inhibitor proteins. (
  • This cytoskeletal protein, originally described in the red cell, forms a ternary complex with spectrin-actin, and attaches the spectrin latticework to membranes by binding to the cytoplasmic domains of key transmembrane proteins. (
  • As little is known about how nuclear CBC mediates the effects of the cap in higher eukaryotes, we have characterized proteins that interact with CBC in HeLa cell nuclear extracts as potential mediators of its function. (
  • HZ wrapped up in the food vacuole membrane binds saturated and polyunsaturated fatty acids (PUFA) and proteins, generates bioactive ligands and is expelled in the blood during schizogony as residual body. (
  • Following HZ/HNE treatment, two critical proteins in cell cycle regulation, p53 and p21, were increased and the retinoblastoma protein, central regulator of G1-to-S-phase transition, was consequently hypophosphorylated, while GATA-1, master transcription factor in erythropoiesis was reduced. (
  • The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. (
  • To understand the molecular basis for the complex antigenic profile of RhD protein, we expressed a series of RhD fusion proteins using different portions of Duffy protein as a tag in erythroleukemic K562 cells. (
  • With these methods, we also show large-scale single-cell analysis of diverse types of live cells (e.g., microbes, cancer cells, blood cells) based on carbohydrates, proteins, chlorophyll, carotenoids, and lipids with unique capabilities that are not possible with fluorescence-based flow cytometry. (
  • Campana's team genetically modified the K562 cells so they carried on their surfaces many copies of two different proteins, 4-1BBL and IL-15. (
  • As a cofactor for apo-copper-zinc superoxide dismutase (apoCuZnSOD), copper protects against free-radical damage to proteins, membrane lipids, and nucleic acids in a wide range of cells and organs. (
  • 1997). Western analysis of secreted proteins from LOXL2-transfected cells detected a protein of approximately 95 kDa, likely a glycosylated LOXL2 protein, and a protein of 63 kDa, which is an extracellular proteolytically processed form of LOXL2 (Akiri et al. (
  • Concomitant multidrug resistance of cells remains the greatest clinical obstacle in the effective treatment of blood and solid tumors. (
  • Sensitivity of human glioma and brain cells to natural killer cell lysis. (
  • As HMBPP is not produced by mammals, this sensitivity is indicative of a role for Vγ9Vδ2 T cells in pathogen elimination ( 5 ). (
  • However, in contrast to T2, background reactivity of CD8(+) T cells responsive to unpulsed K562/A*0201 was regularly found to be negligible, thereby enhancing the sensitivity of the ELISPOT assay, particularly in donors with strong anti-T2 reactivity. (
  • Selection and characterization of BCR-ABL positive cell lines with differential sensitivity to the tyrosine kinase inhibitor STI571: diverse mechanisms of resistance. (
  • A pancreatic cancer cell line (MIA PaCa-2) was chosen and its growth in nude mice and sensitivity to lysis by human lymphokine-activated killer (LAK) cells were characterized. (
  • Expression of the beta- globin genes has not been previously detected in this cell line. (
  • In this report, we describe the isolation of a variant of the K562 cell line that actively expresses beta-globin messenger RNA (mRNA) and polypeptide and shows greatly reduced expression of the delta-globin genes. (
  • Urothelial carcinoma-associated 1 ( UCA1 ) is a lncRNA that was originally cloned and identified in the bladder cancer cell line BLZ-211 29 . (
  • Haplotype-resolved and integrated genome analysis of the cancer cell line HepG2. (
  • Comprehensive, integrated, and phased whole-genome analysis of the primary ENCODE cell line K562. (
  • A K562 cell line expressing high levels of anti-CD19 CARs was generated as a donor cell to transfer the anti-CD19 CARs onto NK cells via trogocytosis. (
  • Please Click Here to inquire about a cell line that is not yet listed. (
  • The resul was decreased expression of cyclin A and D2 retarded cell cycle progression in erythroid cells and the K562 cell line. (
  • ProSci's entire whole cell lysate line are rigorously tested and used in our research methods . (
  • Induction of CYP4F3 by phenol was also observed in differentiated HL-60 cells, in pro-erythroid cell line K562, and ex vivo in human neutrophiles. (
  • Cytotoxic activity of mononuclear cells (MNC) from peripheral blood of healthy donors was determined by 4 hours Cr51-release assay. (
  • However, cellassociated dye aggregates are not efficiently removed by washing and act as reservoirs of unbound dye that can transfer to unlabeled cells present in an assay. (
  • By contrast, the down-regulation of CD44 resulted in decreased proliferation with a G 0 /G 1 arrest of cell cycle in K562 cells according to the MTT assay and the flow cytometric analysis. (
  • A similar trend was noted in regard to K562 cell lysis. (
  • and administration of therapeutic agents modulating the susceptibility fgjhd, IFN-g, GM-CSF, TNF-a, MIP-1a and MIP-1b compared of tumors to NK-cell lysis has been proposed recently. (
  • It also induced morphological changes in the cell membrane, granulation and lysis of exposed cells. (
  • Hypoxia-inducible factor-1 alpha ( HIF-1a ) stimulates LOXL2 mRNA transcription in fibroblasts and renal tubular epithelial cells (Higgins et al. (
  • We found that PD180970 inhibited in vivo tyrosine phosphorylation of p210 Bcr-Abl (IC 50 = 170 n m ) and the p210 Bcr-Abl substrates Gab2 and CrkL (IC 50 = 80 n m ) in human K562 chronic myelogenous leukemic cells. (
  • Gab2 is constitutively tyrosine phosphorylated and associated with the protein tyrosine phosphatase SHP2 in p210 Bcr-Abl -transformed hematopoietic cells such as human CML K562 cells. (
  • We found that a novel pyrido[2,3- d ]pyrimidine derivative (11) , PD180970 (see structure in Fig. 2 A ⇓ ), potently inhibits Gab2 tyrosine phosphorylation in K562 cells. (
  • These findings suggest that PD180970 is a novel Abl tyrosine kinase inhibitor that can selectively affect Bcr-Abl-positive leukemic cells. (
  • Chronic myeloid leukemia (CML) is a stem cell disorder caused by a constitutively activated tyrosine kinase, the Bcr-Abl oncoprotein. (
  • Effects of a selective inhibitor of the Abl tyrosine kinase on the growth of Bcr-Abl positive cells. (
  • The tyrosine kinase inhibitor CGP57148B selectively inhibits the growth of BCR-ABL positive cells. (
  • Mechanism of resistance to the ABL tyrosine kinase inhibitor STI571 in BCR/ABL-transformed hematopoietic cell lines. (
  • Currently, little information is available concerning the effects of simultaneously causing microtubule disruption while inhibiting tyrosine kinase activity in CML cells. (
  • In contrast SV40 large T antigen, which transforms cells through different mechanisms, and the v-Ras and v-Raf oncoproteins, which lie in signaling pathways downstream of tyrosine kinases, do not activate Stat3. (
  • 1994 ) Coordinate regulation of steel factor, its receptor (Kit), and cytoadhesion molecule (ICAM-1 and ELAM-1) mRNA expression in human vascular endothelial cells. (
  • DC-SIGN has specificity for high-mannose moieties ( 6 ), and it functions as an adhesion receptor that establishes cellular interactions with endothelial cells through ICAM-2 ( 7 ) and with T cells through ICAM-3 ( 4 ), probably by recognizing high-mannose moieties on these counterstructures. (
  • Further, we observed that xanthohumol inhibits leukemia cell invasion, metalloprotease production, and adhesion to endothelial cells, potentially preventing in vivo life-threatening complications of leukostasis and tissue infiltration by leukemic cells. (
  • When ceruloplasmin returns to the liver, the sialic acid can be removed by the outer endothelial cells followed by an endocytosis of the desialated protein via the asialoglycoprotein receptor in the liver parenchyma (Irie and Tavassoli 1986). (
  • Cancer patient-derived CIK cells were also expanded after priming with TG, but they expressed lower levels of the NKp46 triggering receptor and NKG2D activating receptor, thus manifesting a reduced ability to lyse K562 cells. (
  • MNC show a reduced capacity to lyse HNE-treated cells also under the enhancing cytolytic effect of IFN. (
  • HK-II expression, glucose uptake, endogenous reactive oxygen species (ROS) level of leukemic cell lines K-562 and THP-1 and then investigated if 3-BP can sensitize the leukemic cells K-562 to anti-leukemic drug Daunorubicin (DNR). (
  • HEL cells were I-i+H+X-. The presence of the I+ population in K562 cells is particularly noteworthy, since I-antigen is characteristic of adult mature erythrocytes and is absent in most human leukemic cell lines. (
  • Imatinib and oxaphorines did not demonstrate any toxicity on normal zebrafish embryos and decreased the leukemic burden in animals xenografted with sensitive leukemic cell lines. (
  • Induction of CYP4F3 by benzene metabolites in human white blood cells in vivo, in human promyelocytic leukemic cell lines, and ex vivo in human blood neutrophils. (
  • Flow cytometry dot plots showing expression of the hematopoietic stem and progenitor cell marker CD34 and megakaryocyte markers CD41a and CD42b (A) before and (B,C) after culture of CD34+ cord blood cells for 14 days in StemSpan™ SFEM containing Megakaryocyte Expansion Supplement. (
  • Using single-cell transcriptome analysis, we have identified a cluster of cells within immature hematopoietic stem and progenitor cell populations that specifically express genes related to the megakaryocyte lineage. (
  • Furthermore, induction of ABCB1 mRNA expression on doxorubicin treatment of naive K562 cells was also accompanied by increased FOXO3a activity. (
  • Moreover, activation of FOXO3a increased ABCB1 drug efflux potential in KD30 cells, whereas silencing of FOXO3a by siRNA significantly reduced ABCB1 drug efflux ability. (
  • Our results showed that resistance of K562/RA cells induced by adriamycin treatment was significantly higher (115.81-fold) than that of parental K562 cells. (
  • In type 1 diabetes, due to pancreatic β-cell death and insulin deficiency, glucose uptake and disposal decrease significantly while endogenous glucose production increases. (
  • Ex vivo-isolated NK cells neoexpressed the activation Ag CD69 and released IFN-γ and TNF-α upon binding with BMSC. (
  • K562 Cells Produce an Anti-Inflammatory Factor That Inhibits Neutrophil Functions In Vivo," Blood 80:1546-1552 (1992). (
  • Moreover, we reported here for the first time in an in vivo model that at-RA reduced, to basal levels, the expression of VLA-4 (α4β1) integrin induced by mercury on peripheral blood leukocytes (PBLs). (
  • CD2, LFA-1, and CD56 were transiently up-regulated on the surface of NK cells exposed to rhIL-12 in vivo . (
  • Ex vivo generation of highly purified and activated natural killer cells from human peripheral blood. (
  • The method provides vectors for selective delivery of nucleic acids to specific cell types in vivo and a means to alter the tropism of an infectious agent. (
  • We found that both K-562 and THP-1 cells have multi-fold high levels of HK-II, glucose uptake and endogenous ROS with respect to normal PBMCs. (
  • Conversely, silencing of endogenous FOXO3a expression in KD225 cells inhibited the expression of this transport protein. (
  • This finding suggests that the activation of the endogenous beta-globin genes results from changes in the trans-acting environment of these cells. (
  • Increased fetal hemoglobin (HbF), whether endogenous or drug induced, ameliorates the symptoms and complications of SCD by preventing red blood cell sickling through diluting HbS and inhibiting its polymerization. (
  • Pathogenic structures such as lipopolysaccharide, lipoteichoic acid, and peptidoglycans, but also endogenous signals like CD40-CD40L ligation by T cells ( 1 ) and TNF-α production by NK cells ( 2 ), trigger DC maturation. (
  • In addition, using various monoclonal antibodies against RhD, we compared the antigenic profile of expressed RhD fusion protein with that of endogenous RhD in K562 cells as well as in erythrocytes. (
  • WB: Recombinant Human Iba1 protein ( ab117478 ), Human peripheral blood mononucleocyte and frontal cortex lysate. (
  • When the nude mice are pretreated with human recombinant tumor necrosis factor, localization of LAK cells compared with PBLs is even further enhanced both in tumors implanted in the pancreas (3.1 +/- 0.5%d/gm vs 0.56 +/- 0.06%d/gm, p less than 0.01) and in the subcutis (12.5 +/- 8.3%d/gm vs 0.95 +/- 0.29%d/gm, p less than 0.001). (
  • In addition to serving as a prosthetic group for enzymes and a hemoglobin structural component, heme is a crucial homeostatic regulator of erythroid cell development and function. (
  • Defective haematopoiesis resulted in a reduced erythroid cell number within the yolk sac. (
  • This finding, from researchers at St. Jude Children's Research Hospital, suggests a way to improve the outcome of children who receive treatment for acute lymphoblastic leukemia (ALL) or other blood cancers. (
  • miR-34a represses the translation of sirtuin 1 (SIRT1) in liver by binding to the 3′-UTR region of SIRT1 messenger RNA, contributing to metabolic syndrome Downregulation of SIRT1 by miR-34a promotes cellular senescence and inflammation in vascular smooth muscle cells of old mice, similar to reduced SIRT1 in vascular smooth muscle cells in humans. (
  • Furthermore, rhIL-12 therapy can reverse defects in NK cell and T-cell function that are associated with advanced cancer in humans. (
  • Value of Monoclonal Anti-CD22 (p135) Antibodies for the Detection of Normal and Neoplastic B Lymphoid Cells," Blood 69:836-840 (1987). (
  • The only curative therapy for sickle cell disease (SCD) is allogeneic hematopoietic stem cell (HSC) transplantation. (
  • Currently available allogeneic or xenogeneic APC (such as the human lymphoid hybrid T2 or HLA-transfected insect cells) can either lead to strong background spot production by APC-reactive T lymphocytes or have a low antigen presentation capability. (
  • To evaluate the safety and feasibility of vaccination with two irradiated allogeneic colorectal carcinoma cells administered with GM-K562 cell vaccine in sequence with an immunomodulatory dose of cyclophosphamide. (
  • 1 and vaccine therapy comprising allogeneic colorectal carcinoma cells and K562/GM-CSF cells intradermally on day 0. (
  • Comparison of Tacrolimus and Sirolimus (Tac/Sir) versus Tacrolimus, Sirolimus, and mini-methotrexate (Tac/Sir/MTX) as acute graft-versus-host disease prophylaxis after reduced-intensity conditioning allogeneic peripheral blood stem cell transplantation. (
  • Incidence, Predictors, and Outcomes of Veno-Occlusive Disease/Sinusoidal Obstruction Syndrome after Reduced-Intensity Allogeneic Hematopoietic Cell Transplantation. (
  • White blood cell recovery after allogeneic hematopoietic cell transplantation predicts clinical outcome. (
  • Moreover, treatment of primary CD34 + cell-derived erythroid cultures with metformin, an FDA-approved drug known to enhance FOXO3 activity in nonerythroid cells, caused dose-related FOXO3-dependent increases in the percentage of HbF protein and the fraction of HbF-immunostaining cells (F cells). (
  • In most cells, NFκB is composed of a heterodimer of p65 and p50, where the p65 protein is responsible for the transactivation potential. (
  • A B7 family protein, butyrophilin 3 isoform A1 (BTN3A1), is required for HMBPP to activate Vγ9Vδ2 T cells ( 12 - 15 ). (
  • PA acts to deliver LF to the cytosol of target cell and LF is a Zn2+-dependent metalloprotease, cleaves mitogen activated protein kinase kinases (MAPKKs) family through proteolysis of their N-terminal. (
  • PARP1 neither influenced binding of HIF-1 to its hypoxic response element nor changed HIF-1α protein levels in hypoxic cells. (
  • Erythropoiesis was inhibited by transferred HNE via blockage of cell cycle and the down-regulation of protein expression of crucial receptors (erythropoietinR, transferrinR and stem-cell-factorR). (
  • HZ and HZ-laden human monocytes inhibited growth of co-cultivated human erythroid cells and produced HNE that diffused to adjacent cells generating HNE-protein adducts. (
  • As a second major effect, HZ and HNE inhibited protein expression of crucial receptors (R): transferrin R1, stem cell factorR, interleukin-3R and erythropoietinR. (
  • Here, we used single-cell mass cytometry and absolute quantification by mass spectrometry to capture the temporal dynamics of TF protein expression in individual cells during human erythropoiesis. (
  • Importantly, upregulation of a megakaryocytic TF in early progenitors is sufficient to deviate cells from an erythroid to a megakaryocyte trajectory, showing that quantitative changes in protein abundance of LS-TFs in progenitors can determine alternate cell fates. (
  • Several studies have indicated that NRF2 potentially regulates genes in other pathways such as protein transport, phosphorylation, cell cycle, and growth [ 11 - 15 ]. (
  • The human D antigens, one of the most clinically important blood groups, are presented by RhD protein with a putative 12 transmembrane topology. (
  • A) siRNA targeting the indicated spliceosome genes or a control non-targeting siRNA were transfected into RAW264.7 mouse macrophages, the cells were then stimulated with 20 ng/mL lipopolysaccharide (LPS) for 6 hours (h), and IL-6 protein production was monitored by ELISA. (
  • The artificial receptor on the NK molecule was designed to recognize a protein called CD19, which is found on the surface of leukemic cells. (
  • Cell-Cycle-Specific Changes in Chromatin Organization Correlate Temporally with Histone H1 Phosphorylation", BBA, 517:486-499, 1978. (
  • We identified a novel role for HIF-1α in the regulation of tone in lung via effects on myosin light chain phosphorylation in the pulmonary artery smooth muscle cells and in regulating expression of the β1 subunit of the calcium sensitive potassium channel. (
  • He is currently chief of the Division of Hematologic Malignancies and codirector of the Adult Stem Cell Transplantation Program. (
  • Dr. Soiffer is chairman of the Center for International Blood and Marrow Transplant Research advisory committee and has served as vice president (2006), president (2007), and immediate past president (2008) of the American Society of Blood and Marrow Transplantation. (
  • Use of matched unrelated donors compared with matched related donors is associated with lower relapse and superior progression-free survival after reduced-intensity conditioning hematopoietic stem cell transplantation. (
  • HLA-C mismatch is associated with inferior survival after unrelated donor non-myeloablative hematopoietic stem cell transplantation. (
  • Early Clinical Predictors of Hepatic Veno-Occlusive Disease/Sinusoidal Obstruction Syndrome after Myeloablative Stem Cell Transplantation. (
  • Bortezomib-based immunosuppression after reduced-intensity conditioning hematopoietic stem cell transplantation: randomized phase II results. (
  • Donor chimerism early after reduced-intensity conditioning hematopoietic stem cell transplantation predicts relapse and survival. (
  • Alterations in the peripheral NK cell repertoire could be correlated to the type of immunosuppression, i.e., calcineurin-inhibitors like Cyclosporin A vs. Tacrolimus with or without addition of mTOR inhibitors. (
  • The presence of calcineurin or mTOR inhibitors had also functional consequences regarding degranulation and interferon-γ-production against K562 target cells, respectively. (
  • B5.2 The carbohydrate moiety of the CD22 antigen can be modulated by inhibitors of the glycosylation pathway," B-cell antigens-papers, 65-67. (
  • K562, Jurkat and NB4 human leukemia cell lines were obtained from the American Tissue Culture Collection and grown in RPMI 1640 medium with glutamax-I (Lonza, Basel, Switzerland) supplemented with 10% (vol/vol) fetal bovine serum (Lonza). (
  • They placed this sample into a dish containing a type of human leukemia cell called K562. (
  • The present invention is concerned with a series of novel monoclonal antibodies directed against CD22, a B lineage-restricted member of the Ig-superfamily which serves as an adhesion receptor expressed by mature B lymphocytes and is believed to function in the regulation of B cell activation. (
  • The monoclonal antibodies (mAb) specifically block red blood cell and leukocyte adhesion (80-100%) to COS cells transfected with CD22 cDNA and also identify a region of CD22 distinct from those defined by previously described CD22 mAb. (
  • The Monoclonal Antibodies .alpha.S-HCL 1 (.alpha.Leu-14) and .alpha.S-HCL 3 (.alpha.Leu-M5) Allow the Diagnosis of Hairy Cell Leukemia," Blood 65(4):974-983 (1985). (
  • The labeling protocol is rapid, simple, and applicable to essentially any membrane-containing cell or particle ( Figure S2 *), but obtaining bright, uniform, and reproducible labeling requires attention to different variables than when staining with antibodies or other equilibrium binding reagents 1-3 . (
  • The presence of donor-specific antibodies was associated with reduced numbers of CD56 dim NK cells. (
  • Bortezomib, a proteasome inhibitor with anti-MM clinical efficacy, activates the DNA damage response to augment MICA expression in some MM cells, thereby enhancing their opsonization by anti-MICA antibodies. (