Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
The flow of BLOOD through or around an organ or region of the body.
A value equal to the total volume flow divided by the cross-sectional area of the vascular bed.
The circulation of blood through the BLOOD VESSELS of the BRAIN.
A technique encompassing morphometry, densitometry, neural networks, and expert systems that has numerous clinical and research applications and is particularly useful in anatomic pathology for the study of malignant lesions. The most common current application of image cytometry is for DNA analysis, followed by quantitation of immunohistochemical staining.
A method of non-invasive, continuous measurement of MICROCIRCULATION. The technique is based on the values of the DOPPLER EFFECT of low-power laser light scattered randomly by static structures and moving tissue particulates.
A scanning microscope-based, cytofluorimetry technique for making fluorescence measurements and topographic analysis on individual cells. Lasers are used to excite fluorochromes in labeled cellular specimens. Fluorescence is detected in multiple discrete wavelengths and the locational data is processed to quantitatively assess APOPTOSIS; PLOIDIES; cell proliferation; GENE EXPRESSION; PROTEIN TRANSPORT; and other cellular processes.
Small uniformly-sized spherical particles, of micrometer dimensions, frequently labeled with radioisotopes or various reagents acting as tags or markers.
The force that opposes the flow of BLOOD through a vascular bed. It is equal to the difference in BLOOD PRESSURE across the vascular bed divided by the CARDIAC OUTPUT.
Rhythmic, intermittent propagation of a fluid through a BLOOD VESSEL or piping system, in contrast to constant, smooth propagation, which produces laminar flow.
Unstable isotopes of xenon that decay or disintegrate emitting radiation. Xe atoms with atomic weights 121-123, 125, 127, 133, 135, 137-145 are radioactive xenon isotopes.
Process of classifying cells of the immune system based on structural and functional differences. The process is commonly used to analyze and sort T-lymphocytes into subsets based on CD antigens by the technique of flow cytometry.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
The movement and the forces involved in the movement of the blood through the CARDIOVASCULAR SYSTEM.
Elements of limited time intervals, contributing to particular results or situations.
The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)
The circulation of the BLOOD through the MICROVASCULAR NETWORK.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.
Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.
The circulation of the BLOOD through the vessels of the KIDNEY.
The rate at which oxygen is used by a tissue; microliters of oxygen STPD used per milligram of tissue per hour; the rate at which oxygen enters the blood from alveolar gas, equal in the steady state to the consumption of oxygen by tissue metabolism throughout the body. (Stedman, 25th ed, p346)
The physiological widening of BLOOD VESSELS by relaxing the underlying VASCULAR SMOOTH MUSCLE.
The study of the deformation and flow of matter, usually liquids or fluids, and of the plastic flow of solids. The concept covers consistency, dilatancy, liquefaction, resistance to flow, shearing, thixotrophy, and VISCOSITY.
The circulation of BLOOD through the LIVER.
A cell line derived from cultured tumor cells.
The circulation of the BLOOD through the LUNGS.
Tomography using radioactive emissions from injected RADIONUCLIDES and computer ALGORITHMS to reconstruct an image.
The degree of replication of the chromosome set in the karyotype.
The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.
The circulation of blood through the BLOOD VESSELS supplying the abdominal VISCERA.
A colorless, odorless gas that can be formed by the body and is necessary for the respiration cycle of plants and animals.
The presence of an increased amount of blood in a body part or an organ leading to congestion or engorgement of blood vessels. Hyperemia can be due to increase of blood flow into the area (active or arterial), or due to obstruction of outflow of blood from the area (passive or venous).
Unstable isotopes of oxygen that decay or disintegrate emitting radiation. O atoms with atomic weights 13, 14, 15, 19, and 20 are radioactive oxygen isotopes.
The complex series of phenomena, occurring between the end of one CELL DIVISION and the end of the next, by which cellular material is duplicated and then divided between two daughter cells. The cell cycle includes INTERPHASE, which includes G0 PHASE; G1 PHASE; S PHASE; and G2 PHASE, and CELL DIVISION PHASE.
Ultrasonography applying the Doppler effect, with frequency-shifted ultrasound reflections produced by moving targets (usually red blood cells) in the bloodstream along the ultrasound axis in direct proportion to the velocity of movement of the targets, to determine both direction and velocity of blood flow. (Stedman, 25th ed)
The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts.
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
The number of times the HEART VENTRICLES contract per unit of time, usually per minute.
The relationship between the dose of an administered drug and the response of the organism to the drug.
Antibodies produced by a single clone of cells.
A noble gas with the atomic symbol Xe, atomic number 54, and atomic weight 131.30. It is found in the earth's atmosphere and has been used as an anesthetic.
The change in gene frequency in a population due to migration of gametes or individuals (ANIMAL MIGRATION) across population barriers. In contrast, in GENETIC DRIFT the cause of gene frequency changes are not a result of population or gamete movement.
Determination of the shortest time interval between the injection of a substance in the vein and its arrival at some distant site in sufficient concentration to produce a recognizable end result. It represents approximately the inverse of the average velocity of blood flow between two points.
Ultrasonography applying the Doppler effect, with the superposition of flow information as colors on a gray scale in a real-time image. This type of ultrasonography is well-suited to identifying the location of high-velocity flow (such as in a stenosis) or of mapping the extent of flow in a certain region.
Volume of circulating BLOOD. It is the sum of the PLASMA VOLUME and ERYTHROCYTE VOLUME.
Recording of change in the size of a part as modified by the circulation in it.
The vessels carrying blood away from the heart.
Drugs used to cause dilation of the blood vessels.
Quaternary ammonium analog of ethidium; an intercalating dye with a specific affinity to certain forms of DNA and, used as diiodide, to separate them in density gradients; also forms fluorescent complexes with cholinesterase which it inhibits.
Maintenance of blood flow to an organ despite obstruction of a principal vessel. Blood flow is maintained through small vessels.
The arterial blood vessels supplying the CEREBRUM.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Fluorescent probe capable of being conjugated to tissue and proteins. It is used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.
A free radical gas produced endogenously by a variety of mammalian cells, synthesized from ARGININE by NITRIC OXIDE SYNTHASE. Nitric oxide is one of the ENDOTHELIUM-DEPENDENT RELAXING FACTORS released by the vascular endothelium and mediates VASODILATION. It also inhibits platelet aggregation, induces disaggregation of aggregated platelets, and inhibits platelet adhesion to the vascular endothelium. Nitric oxide activates cytosolic GUANYLATE CYCLASE and thus elevates intracellular levels of CYCLIC GMP.
Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.
Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)
The physiological narrowing of BLOOD VESSELS by contraction of the VASCULAR SMOOTH MUSCLE.
Theoretical representations that simulate the behavior or activity of the cardiovascular system, processes, or phenomena; includes the use of mathematical equations, computers and other electronic equipment.
The minute vessels that connect the arterioles and venules.
Either of two extremities of four-footed non-primate land animals. It usually consists of a FEMUR; TIBIA; and FIBULA; tarsals; METATARSALS; and TOES. (From Storer et al., General Zoology, 6th ed, p73)
The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality.
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
A hypoperfusion of the BLOOD through an organ or tissue caused by a PATHOLOGIC CONSTRICTION or obstruction of its BLOOD VESSELS, or an absence of BLOOD CIRCULATION.
A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes.
A non-invasive technique using ultrasound for the measurement of cerebrovascular hemodynamics, particularly cerebral blood flow velocity and cerebral collateral flow. With a high-intensity, low-frequency pulse probe, the intracranial arteries may be studied transtemporally, transorbitally, or from below the foramen magnum.
The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.
High-molecular weight glycoproteins uniquely expressed on the surface of LEUKOCYTES and their hemopoietic progenitors. They contain a cytoplasmic protein tyrosine phosphatase activity which plays a role in intracellular signaling from the CELL SURFACE RECEPTORS. The CD45 antigens occur as multiple isoforms that result from alternative mRNA splicing and differential usage of three exons.
A family of spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one derivatives. These are used as dyes, as indicators for various metals, and as fluorescent labels in immunoassays.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
The pressure that would be exerted by one component of a mixture of gases if it were present alone in a container. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES).
The blood vessels which supply and drain the RETINA.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Mature LYMPHOCYTES and MONOCYTES transported by the blood to the body's extravascular space. They are morphologically distinguishable from mature granulocytic leukocytes by their large, non-lobed nuclei and lack of coarse, heavily stained cytoplasmic granules.
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Established cell cultures that have the potential to propagate indefinitely.
Measurement of oxygen and carbon dioxide in the blood.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
The inferior part of the lower extremity between the KNEE and the ANKLE.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
A subtype of striated muscle, attached by TENDONS to the SKELETON. Skeletal muscles are innervated and their movement can be consciously controlled. They are also called voluntary muscles.
The property of emitting radiation while being irradiated. The radiation emitted is usually of longer wavelength than that incident or absorbed, e.g., a substance can be irradiated with invisible radiation and emit visible light. X-ray fluorescence is used in diagnosis.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible.
Treatment process involving the injection of fluid into an organ or tissue.
The largest of the cerebral arteries. It trifurcates into temporal, frontal, and parietal branches supplying blood to most of the parenchyma of these lobes in the CEREBRAL CORTEX. These are the areas involved in motor, sensory, and speech activities.
Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely.
The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.
Extracellular vesicles generated by the shedding of CELL MEMBRANE blebs.
The number of CELLS of a specific kind, usually measured per unit volume or area of sample.
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
Any of the ruminant mammals with curved horns in the genus Ovis, family Bovidae. They possess lachrymal grooves and interdigital glands, which are absent in GOATS.
A series of progressive, overlapping events, triggered by exposure of the PLATELETS to subendothelial tissue. These events include shape change, adhesiveness, aggregation, and release reactions. When carried through to completion, these events lead to the formation of a stable hemostatic plug.
The number of LYMPHOCYTES per unit volume of BLOOD.
A nucleoside that is composed of ADENINE and D-RIBOSE. Adenosine or adenosine derivatives play many important biological roles in addition to being components of DNA and RNA. Adenosine itself is a neurotransmitter.
An analgesic and antipyretic that has been given by mouth and as ear drops. Antipyrine is often used in testing the effects of other drugs or diseases on drug-metabolizing enzymes in the liver. (From Martindale, The Extra Pharmacopoeia, 30th ed, p29)
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
Localized reduction of blood flow to brain tissue due to arterial obstruction or systemic hypoperfusion. This frequently occurs in conjunction with brain hypoxia (HYPOXIA, BRAIN). Prolonged ischemia is associated with BRAIN INFARCTION.
A protein of the annexin family isolated from human PLACENTA and other tissues. It inhibits cytosolic PHOSPHOLIPASE A2, and displays anticoagulant activity.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
A statistical technique that isolates and assesses the contributions of categorical independent variables to variation in the mean of a continuous dependent variable.
Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.
Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.
The processes whereby the internal environment of an organism tends to remain balanced and stable.
The thin, highly vascular membrane covering most of the posterior of the eye between the RETINA and SCLERA.
The TEMPERATURE at the outer surface of the body.
A classification of lymphocytes based on structurally or functionally different populations of cells.
Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.
The neural systems which act on VASCULAR SMOOTH MUSCLE to control blood vessel diameter. The major neural control is through the sympathetic nervous system.
The main artery of the thigh, a continuation of the external iliac artery.
DNA present in neoplastic tissue.
Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation.
Unstable isotopes of nitrogen that decay or disintegrate emitting radiation. N atoms with atomic weights 12, 13, 16, 17, and 18 are radioactive nitrogen isotopes.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.
Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
A method of computed tomography that uses radionuclides which emit a single photon of a given energy. The camera is rotated 180 or 360 degrees around the patient to capture images at multiple positions along the arc. The computer is then used to reconstruct the transaxial, sagittal, and coronal images from the 3-dimensional distribution of radionuclides in the organ. The advantages of SPECT are that it can be used to observe biochemical and physiological processes as well as size and volume of the organ. The disadvantage is that, unlike positron-emission tomography where the positron-electron annihilation results in the emission of 2 photons at 180 degrees from each other, SPECT requires physical collimation to line up the photons, which results in the loss of many available photons and hence degrades the image.
A powerful vasodilator used in emergencies to lower blood pressure or to improve cardiac function. It is also an indicator for free sulfhydryl groups in proteins.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
A classification of T-lymphocytes, especially into helper/inducer, suppressor/effector, and cytotoxic subsets, based on structurally or functionally different populations of cells.
A gamma-emitting RADIONUCLIDE IMAGING agent used in the evaluation of regional cerebral blood flow and in non-invasive dynamic biodistribution studies and MYOCARDIAL PERFUSION IMAGING. It has also been used to label leukocytes in the investigation of INFLAMMATORY BOWEL DISEASES.
Compounds that contain the radical R2C=N.OH derived from condensation of ALDEHYDES or KETONES with HYDROXYLAMINE. Members of this group are CHOLINESTERASE REACTIVATORS.
A critical subpopulation of regulatory T-lymphocytes involved in MHC Class I-restricted interactions. They include both cytotoxic T-lymphocytes (T-LYMPHOCYTES, CYTOTOXIC) and CD8+ suppressor T-lymphocytes.
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
A technique of inputting two-dimensional images into a computer and then enhancing or analyzing the imagery into a form that is more useful to the human observer.
Relatively complete absence of oxygen in one or more tissues.
The smallest divisions of the arteries located between the muscular arteries and the capillaries.
Artery originating from the internal carotid artery and distributing to the eye, orbit and adjacent facial structures.
Non-invasive method of demonstrating internal anatomy based on the principle that atomic nuclei in a strong magnetic field absorb pulses of radiofrequency energy and emit them as radiowaves which can be reconstructed into computerized images. The concept includes proton spin tomographic techniques.
A noble gas that is found in the atmosphere. It has the atomic symbol Kr, atomic number 36, atomic weight 83.80, and has been used in electric bulbs.
A non-selective inhibitor of nitric oxide synthase. It has been used experimentally to induce hypertension.
Compounds or agents that combine with an enzyme in such a manner as to prevent the normal substrate-enzyme combination and the catalytic reaction.
One of the CARBONIC ANHYDRASE INHIBITORS that is sometimes effective against absence seizures. It is sometimes useful also as an adjunct in the treatment of tonic-clonic, myoclonic, and atonic seizures, particularly in women whose seizures occur or are exacerbated at specific times in the menstrual cycle. However, its usefulness is transient often because of rapid development of tolerance. Its antiepileptic effect may be due to its inhibitory effect on brain carbonic anhydrase, which leads to an increased transneuronal chloride gradient, increased chloride current, and increased inhibition. (From Smith and Reynard, Textbook of Pharmacology, 1991, p337)
The major interferon produced by mitogenically or antigenically stimulated LYMPHOCYTES. It is structurally different from TYPE I INTERFERON and its major activity is immunoregulation. It has been implicated in the expression of CLASS II HISTOCOMPATIBILITY ANTIGENS in cells that do not normally produce them, leading to AUTOIMMUNE DISEASES.
A purely physical condition which exists within any material because of strain or deformation by external forces or by non-uniform thermal expansion; expressed quantitatively in units of force per unit area.
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
An optical source that emits photons in a coherent beam. Light Amplification by Stimulated Emission of Radiation (LASER) is brought about using devices that transform light of varying frequencies into a single intense, nearly nondivergent beam of monochromatic radiation. Lasers operate in the infrared, visible, ultraviolet, or X-ray regions of the spectrum.
An NADPH-dependent enzyme that catalyzes the conversion of L-ARGININE and OXYGEN to produce CITRULLINE and NITRIC OXIDE.
The hollow thick-walled muscular organ in the female PELVIS. It consists of the fundus (the body) which is the site of EMBRYO IMPLANTATION and FETAL DEVELOPMENT. Beyond the isthmus at the perineal end of fundus, is CERVIX UTERI (the neck) opening into VAGINA. Beyond the isthmi at the upper abdominal end of fundus, are the FALLOPIAN TUBES.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
Central retinal artery and its branches. It arises from the ophthalmic artery, pierces the optic nerve and runs through its center, enters the eye through the porus opticus and branches to supply the retina.
Studies which start with the identification of persons with a disease of interest and a control (comparison, referent) group without the disease. The relationship of an attribute to the disease is examined by comparing diseased and non-diseased persons with regard to the frequency or levels of the attribute in each group.
Complex of at least five membrane-bound polypeptides in mature T-lymphocytes that are non-covalently associated with one another and with the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL). The CD3 complex includes the gamma, delta, epsilon, zeta, and eta chains (subunits). When antigen binds to the T-cell receptor, the CD3 complex transduces the activating signals to the cytoplasm of the T-cell. The CD3 gamma and delta chains (subunits) are separate from and not related to the gamma/delta chains of the T-cell receptor (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA).
Glycoproteins found on the membrane or surface of cells.
Adherence of cells to surfaces or to other cells.
A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
Protein analogs and derivatives of the Aequorea victoria green fluorescent protein that emit light (FLUORESCENCE) when excited with ULTRAVIOLET RAYS. They are used in REPORTER GENES in doing GENETIC TECHNIQUES. Numerous mutants have been made to emit other colors or be sensitive to pH.
Any of the tubular vessels conveying the blood (arteries, arterioles, capillaries, venules, and veins).
The internal resistance of the BLOOD to shear forces. The in vitro measure of whole blood viscosity is of limited clinical utility because it bears little relationship to the actual viscosity within the circulation, but an increase in the viscosity of circulating blood can contribute to morbidity in patients suffering from disorders such as SICKLE CELL ANEMIA and POLYCYTHEMIA.
Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.
The domestic cat, Felis catus, of the carnivore family FELIDAE, comprising over 30 different breeds. The domestic cat is descended primarily from the wild cat of Africa and extreme southwestern Asia. Though probably present in towns in Palestine as long ago as 7000 years, actual domestication occurred in Egypt about 4000 years ago. (From Walker's Mammals of the World, 6th ed, p801)
The hollow, muscular organ that maintains the circulation of the blood.
Theoretical representations that simulate the behavior or activity of biological processes or diseases. For disease models in living animals, DISEASE MODELS, ANIMAL is available. Biological models include the use of mathematical equations, computers, and other electronic equipment.
A clinical manifestation of abnormal increase in the amount of carbon dioxide in arterial blood.
Ultrasonography applying the Doppler effect, with velocity detection combined with range discrimination. Short bursts of ultrasound are transmitted at regular intervals and the echoes are demodulated as they return.
Expenditure of energy during PHYSICAL ACTIVITY. Intensity of exertion may be measured by rate of OXYGEN CONSUMPTION; HEAT produced, or HEART RATE. Perceived exertion, a psychological measure of exertion, is included.
Substances that inhibit or prevent the proliferation of NEOPLASMS.
A genus of the subfamily CERCOPITHECINAE, family CERCOPITHECIDAE, consisting of five named species: PAPIO URSINUS (chacma baboon), PAPIO CYNOCEPHALUS (yellow baboon), PAPIO PAPIO (western baboon), PAPIO ANUBIS (or olive baboon), and PAPIO HAMADRYAS (hamadryas baboon). Members of the Papio genus inhabit open woodland, savannahs, grassland, and rocky hill country. Some authors consider MANDRILLUS a subgenus of Papio.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Either of the two principal arteries on both sides of the neck that supply blood to the head and neck; each divides into two branches, the internal carotid artery and the external carotid artery.
The volume of packed RED BLOOD CELLS in a blood specimen. The volume is measured by centrifugation in a tube with graduated markings, or with automated blood cell counters. It is an indicator of erythrocyte status in disease. For example, ANEMIA shows a low value; POLYCYTHEMIA, a high value.
The chromosomal constitution of cells which deviate from the normal by the addition or subtraction of CHROMOSOMES, chromosome pairs, or chromosome fragments. In a normally diploid cell (DIPLOIDY) the loss of a chromosome pair is termed nullisomy (symbol: 2N-2), the loss of a single chromosome is MONOSOMY (symbol: 2N-1), the addition of a chromosome pair is tetrasomy (symbol: 2N+2), the addition of a single chromosome is TRISOMY (symbol: 2N+1).
A state characterized by loss of feeling or sensation. This depression of nerve function is usually the result of pharmacologic action and is induced to allow performance of surgery or other painful procedures.
The cells found in the body fluid circulating throughout the CARDIOVASCULAR SYSTEM.
Chemicals and substances that impart color including soluble dyes and insoluble pigments. They are used in INKS; PAINTS; and as INDICATORS AND REAGENTS.
Pressure within the cranial cavity. It is influenced by brain mass, the circulatory system, CSF dynamics, and skull rigidity.
Glycoproteins found on immature hematopoietic cells and endothelial cells. They are the only molecules to date whose expression within the blood system is restricted to a small number of progenitor cells in the bone marrow.
The circulation of blood through the CORONARY VESSELS of the HEART.
The unborn young of a viviparous mammal, in the postembryonic period, after the major structures have been outlined. In humans, the unborn young from the end of the eighth week after CONCEPTION until BIRTH, as distinguished from the earlier EMBRYO, MAMMALIAN.
Antigens on surfaces of cells, including infectious or foreign cells or viruses. They are usually protein-containing groups on cell membranes or walls and may be isolated.
Cell adhesion molecule and CD antigen that mediates the adhesion of neutrophils and monocytes to activated platelets and endothelial cells.
Regional infusion of drugs via an arterial catheter. Often a pump is used to impel the drug through the catheter. Used in therapy of cancer, upper gastrointestinal hemorrhage, infection, and peripheral vascular disease.
Delivery of drugs into an artery.
Freedom from activity.
Organic compounds that contain technetium as an integral part of the molecule. These compounds are often used as radionuclide imaging agents.
Disease having a short and relatively severe course.
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.
Abnormally low BLOOD PRESSURE that can result in inadequate blood flow to the brain and other vital organs. Common symptom is DIZZINESS but greater negative impacts on the body occur when there is prolonged depravation of oxygen and nutrients.
The thoracolumbar division of the autonomic nervous system. Sympathetic preganglionic fibers originate in neurons of the intermediolateral column of the spinal cord and project to the paravertebral and prevertebral ganglia, which in turn project to target organs. The sympathetic nervous system mediates the body's response to stressful situations, i.e., the fight or flight reactions. It often acts reciprocally to the parasympathetic system.
A vessel that directly interconnects an artery and a vein, and that acts as a shunt to bypass the capillary bed. Not to be confused with surgical anastomosis, nor with arteriovenous fistula.
A short pro-domain caspase that plays an effector role in APOPTOSIS. It is activated by INITIATOR CASPASES such as CASPASE 9. Isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA.
The circulation of BLOOD, of both the mother and the FETUS, through the PLACENTA.
Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
The analysis of a chemical substance by inserting a sample into a carrier stream of reagent using a sample injection valve that propels the sample downstream where mixing occurs in a coiled tube, then passes into a flow-through detector and a recorder or other data handling device.
Physical activity which is usually regular and done with the intention of improving or maintaining PHYSICAL FITNESS or HEALTH. Contrast with PHYSICAL EXERTION which is concerned largely with the physiologic and metabolic response to energy expenditure.
Glycolipid-anchored membrane glycoproteins expressed on cells of the myelomonocyte lineage including monocytes, macrophages, and some granulocytes. They function as receptors for the complex of lipopolysaccharide (LPS) and LPS-binding protein.
The blood DCs are typically identified and enumerated in flow cytometry. Three types of DCs have been defined in human blood: ... "Nomenclature of monocytes and dendritic cells in blood" (PDF). Blood. 116 (16): e74-80. doi:10.1182/blood-2010-02-258558. hdl: ... The disease may also present as a pDC leukemia, i.e. increased levels of malignant pDC in blood (i.e. >2% of nucleated cells) ... They can also be found in an immature state in the blood. Once activated, they migrate to the lymph nodes where they interact ...
April 2017). "Flow Cytometric Measurement of Blood Cells with BCR-ABL1 Fusion Protein in Chronic Myeloid Leukemia". Scientific ... PLA can also be combined with other read out forms such as ELISA, flow cytometry. and Western blotting Gullberg M, ... Leuchowius KJ, Weibrecht I, Landegren U, Gedda L, Söderberg O (October 2009). "Flow cytometric in situ proximity ligation ... Cytometry. Part A. 75 (10): 833-9. doi:10.1002/cyto.a.20771. PMID 19650109. S2CID 2550136. Löf L, Arngården L, Olsson-Strömberg ...
Blood. 104 (1): 250-5. doi:10.1182/blood-2004-01-0181. PMID 15016649. "Clinical Flow Cytometry Case #54". Archived from the ... It is also possible to definitively diagnose hairy cell leukemia through flow cytometry on blood or bone marrow. The hairy ... People with low numbers of red blood cells or platelets may also receive red blood cells and platelets through blood ... A drop in blood counts is usually seen during the first one to two months of treatment. Most patients find that their blood ...
Measured by flow cytometry: Normal values ,2.5% total T cells; ,1% of total lymphocytes in peripheral blood ... "Blood. 108 (6): 1965-71. doi:10.1182/blood-2006-01-010124. PMC 1895548 . PMID 16757690.. [unreliable medical source?] ... Blood. 105 (6): 2443-8. doi:10.1182/blood-2004-09-3542. PMID 15542578.. [unreliable medical source?] ... Blood. 113 (13): 3027-30. doi:10.1182/blood-2008-09-179630. PMID 19176318.. [unreliable medical source?] ...
Howell-Jolly bodies can be identified and quantified using a blood smear or by flow cytometry. A high number of Howell-Jolly ... where large amounts of blood pool in the spleen but do not flow within vasculature. This lack of blood flow can cause atrophy ... Autosplenectomy can occur in cases of sickle-cell disease where the misshapen cells block blood flow to the spleen, causing ... One of the spleen's main tasks is to filter the blood and remove and recycle damaged or old red blood cells. Splenic function ...
Hutter KJ, Stöhr M (1982). "Rapid detection of mutagen induced micronucleated erythrocytes by flow cytometry". Primary. ... in the red blood cells of salmon. Unlike mammalian red blood cells, those of other vertebrates still contain nuclei. The ... "Enumeration of micronucleated reticulocytes in rat peripheral blood: a flow cytometric study". Primary. Mutation Research. 465 ... Eukaryotes usually have a single nucleus, but a few cell types, such as mammalian red blood cells, have no nuclei, and a few ...
It is impossible to distinguish between T cells and B cells in a peripheral blood smear. Normally, flow cytometry testing is ... "The Challenge of Distinguishing Cell-Cell Complexes from Singlet Cells in Non-Imaging Flow Cytometry and Single-Cell Sorting". ... A type of immune cell that is made in the bone marrow and is found in the blood and in lymph tissue. The two main types of ... In normal situations, the coarse, dense nucleus of a lymphocyte is approximately the size of a red blood cell (about 7 μm in ...
Hult, Annika K; Frame, Tim; Chesla, Scott; Henry, Stephen; Olsson, Martin L (2012). "Flow cytometry evaluation of red blood ... Diagnostic serological analyses including flow cytometry and scanning electron microscopy usually can't see a difference ... For red blood cells incubation for 2 hours at 37 °C achieves >95% FSL insertion with at least 50% insertion being achieved ... For red blood "kodecytes" reliable monitoring of the presence of the "kodecytes" for up to 3 days post intravenous ...
IgG or IgM-coated RBCs and enhance the detection by flow cytometry The flow cytometry is able to detect minor variance in blood ... Flow cytometry is the method of choice. By examining markers on the surface of blood cells, the method can determine whether ... Blood transfusion begins by the withdrawal of 1 to 4 units of blood (1 unit = 450 ml of blood) several weeks before competition ... Blood doping can be achieved by making the body produce more red blood cells itself using drugs, giving blood transfusions ...
... such as the determination of the amount of blood cells in blood via flow cytometry. Plasma was already well known when ... Blood plasma is a yellowish liquid component of blood that holds the blood cells of whole blood in suspension. It is the liquid ... Blood plasma has a density of approximately 1025 kg/m3, or 1.025 g/ml. Blood serum is blood plasma without clotting factors. ... Blood plasma is separated from the blood by spinning a tube of fresh blood containing an anticoagulant in a centrifuge until ...
Early flow cytometry devices shot beams of light at cells in specific wavelengths and measured the resulting absorbance, ... Blood is composed of a fluid portion, called plasma, and a cellular portion that contains red blood cells, white blood cells ... which interferes with red blood cell measurements by causing white blood cells to be counted as red cells). Red and white blood ... In its broadest sense, the term flow cytometry refers to any measurement of the properties of individual cells in a fluid ...
Early flow cytometry devices shot beams of light at cells in specific wavelengths and measured the resulting absorbance, ... The white blood cell differential is a common blood test that is often ordered alongside a complete blood count. The test may ... The test is performed on whole blood, meaning blood that has not been centrifuged. In a manual differential, a stained blood ... where reagents are applied to lyse red blood cells and preserve white blood cells. The sample is diluted and passed into a flow ...
... have elevated CEC levels found within the blood. Using immunomagnetic separation or flow cytometry, CEC levels can be ... The plaque that stays lodged in the coronary arteries may restrict blood flow to the cardiac muscle. This causes ischemia; the ... Blood 93: 2951-2958 Thygesen, K., Alpert, J. S., & White, H. D. (2007). Universal definition of myocardial infarction. Journal ... Blood 93: 2951-2958. Thygesen, K., Alpert, J. S., & White, H. D. (2007). Universal definition of myocardial infarction. Journal ...
... multimer panel tests the proficiency of a given laboratory in performing the flow cytometry based MHC multimer assay on blood ... Cancer Testis Antigen CD1d Dextramer - These MHC Dextramers are flow cytometry reagents for the identification and sorting of ... specific and accurate detection of antigen-specific T cells by using Flow cytometry. The MHC Dextramers covers human, mouse, ... Dextramer CMV kit - These MHC Dextramers provide a method for quantification of CMV-specific CD8+ T cells in whole blood ...
Hult, Annika K; Frame, Tim; Chesla, Scott; Henry, Stephen; Olsson, Martin L (2012). "Flow cytometry evaluation of red blood ... FSL blood group A as a solution has been used to neutralise circulating antibodies in a mouse model and allow incompatible ... For red blood cells, at 37 °C incubation for 2 hours achieves >95% insertion with at least 50% insertion being achieved within ... FSL have been used to create human red cell kodecytes that have been used to detect and identify blood group allo-antibodies as ...
"Flow visualization and flow cytometry with holographic video microscopy". Optics Express. 17 (15): 13071-13079. Bibcode: ... Red blood cell analysis. Phase shift images have been used to study red blood cell dynamics. Red blood cell volume and ... Flow cytometry and particle tracking and characterization. Images created by digital holography are calculated from the ... "Imaging red blood cell dynamics by quantitative phase microscopy" (PDF). Blood Cells, Molecules and Diseases. 41 (1): 10-16. ...
There are now a range of blood tests that can be utilised to aid in the diagnosis of lymphoma. Flow cytometry detects ... Flow cytometry in veterinary oncology. Reggeti F, Bienzle, D. Vet Pathol. 2011 Jan;48(1):223-35 Utility of polymerase chain ... The white blood cell count must be monitored. Prednisone used alone can work very well for weeks to months, but it may cause ... Certain blood tests have also been shown to be prognostic. The stage of the disease is important to treatment and prognosis. ...
... in particular flow cytometry, should be performed unless clinically unnecessary.[citation needed] A peripheral blood smear ... The combination of the microscopic examination of the peripheral blood and analysis of the lymphocytes by flow cytometry to ... of adults with normal blood counts". Blood. 100 (2): 635-9. doi:10.1182/blood.V100.2.635. PMID 12091358. Turgeon, Mary Louise ( ... Both are easily accomplished on a small amount of blood. A flow cytometer instrument can examine the expression of molecules on ...
Flow cytometry immunophenotyping of blood cells to detect clonal phenotypes of plasma cells seen in multiple myeloma (e.g. the ... Monoclonal IgM myeloma proteins operating through their effects on increasing blood hyperviscosity can reduce blood flow to the ... or free λ light chains may increase blood viscosity, deposit in peripheral blood vessels, and thereby cause vascurlar occlusion ... decreases in any white blood cell count, cold agglutinin disease, hyperviscosity of blood, lymphadenopathy, hepatomegaly, ...
Flow cytometry immunophenotyping of blood cells to detect clonal phenotypes of plasma cells seen in multiple myeloma (e.g. the ... The International Myeloma Working Group has defined the diagnostic criteria for plasma cell leukemia as the presence in blood ... levels in the blood due to excess bone re-absorption and/or renal failure; b) higher levels of serum lactate dehydrogenase and ... a disease involving the malignant degeneration of a subtype of white blood cells called plasma cells. It is the terminal stage ...
... measured in a serum protein electrophoresis test or peripheral blood flow cytometry. Such an expansion is said to be " ...
Each flow cytometry pattern is referenced to the patients non-stimulated cells. In addition, a control blood is included in ... The level of reactive oxygen radicals is determined by flow cytometry. Usually a minimum of 5mL whole blood collected in a ... Blood 77:673-686. Rothe G, Oser A and G. Valet. 1988. Dihydrorhodamin 123: a new flow cytometric indicator for respiratory ... Neutophil function in elderly persons assessed by flow cytometry. Immunol. Invest. 25(3): 185-190. Gessler, P., Nebe, T. Birle ...
... in monoclonal gammopathy of uncertain significance and smoldering multiple myeloma based on multiparameter flow cytometry ... Blood. 112 (8): 3122-5. doi:10.1182/blood-2008-06-164228. PMC 2569167. PMID 18669874. Pérez-Persona E, Vidriales MB, Mateo G, ... Blood. 117 (21): 5573-5581. doi:10.1182/blood-2011-01-270140. PMC 3316455. PMID 21441462. Dinarello CA (2011). "Interleukin-1 ... doi:10.1182/blood-2007-05-088443. PMID 17576818. Kyle RA, Durie BG, Rajkumar SV, Landgren O, Blade J, Merlini G, Kröger N, ...
Hutter KJ, Stöhr M (1982). "Rapid detection of mutagen induced micronucleated erythrocytes by flow cytometry". Histochemistry. ... "Enumeration of micronucleated reticulocytes in rat peripheral blood: a flow cytometric study". Mutation Research. 465 (1-2): 91 ... in the red blood cells of salmon.[1] Unlike mammalian red blood cells, those of other vertebrates still contain nuclei. ... In most types of granulocyte, a white blood cell, the nucleus is lobated and can be bi-lobed, tri-lobed or multi-lobed. ...
... the automatic counters also measure the size of the red blood cells by flow cytometry, which is an important tool in ... Anemia can be caused by blood loss, decreased red blood cell production, and increased red blood cell breakdown. Causes of ... Anemia (also spelled anaemia) is a decrease in the total amount of red blood cells (RBCs) or hemoglobin in the blood, or a ... The name is derived from Ancient Greek: ἀναιμία anaimia, meaning "lack of blood", from ἀν- an-, "not" and αἷμα haima, "blood". ...
Determination of monocyte count by hematological analyzers, manual method and flow cytometry in polish population Central ... are for total blood and not only blood plasma. These values are for total blood and not only blood plasma. Acute phase proteins ... An exception is for acid-base and blood gases, which are generally given for arterial blood.[citation needed] Still, the blood ... and not venous which otherwise is standard for other blood tests. Acid-base and blood gases are among the few blood ...
doi:10.1182/blood-2003-06-2070. PMID 14630824. S2CID 1984056. Media related to Flow cytometry at Wikimedia Commons Flow+ ... uses flow cytometry Cell cycle analysis Coulter counter Cytometry Dielectrophoresis Flow Cytometry Standard Mass cytometry ... Flow cytometry is routinely used in basic research, clinical practice, and clinical trials. Uses for flow cytometry include: ... Flow cytometry cell sorters have a collection system unlike flow cytometry analyzers. The collection process starts when a ...
Marrow or blood is examined under light microscopy, as well as flow cytometry, to diagnose the presence of leukemia, to ... Velpeau noted the blood of this person had a consistency "like gruel", and speculated the appearance of the blood was due to ... In straightforward cases, the presence of certain morphologic features (such as Auer rods) or specific flow cytometry results ... Unless otherwise specified in boxes, reference is: Page 97 in: Sun T (2008). Flow cytometry and immunohistochemistry for ...
"Cytofluorometric methods for assessing absolute numbers of cell subsets in blood". Cytometry. 42 (6): 327-346. doi:10.1002/1097 ... Flow Cytometry Standard (FCS) was developed in 1984 to allow recording and sharing of flow cytometry data. Since then, FCS ... Recently, a GenePattern Flow Cytometry Suite has been developed in order to bring advanced flow cytometry data analysis tools ... Flow cytometry Bioinformatics Proteomics Flow Cytometry Standard This article was adapted from the following source under a CC ...
Flow cytometry and FISH to measure the average length of telomeres (flow FISH). Nat Protoc 2006; 1:2365-2376. Moyzis, R.K. et ... Flow-FISH thus allows for a higher throughput analysis of telomere length in blood leukocytes, which are a readily available ... Cytometry A 55, 1-6 (2003). Wieser, M. et al. Nuclear flow FISH: isolation of cell nuclei improves the determination of ... Telomere length dynamics in human lymphocyte subpopulations measured by flow cytometry. Nature Biotechnol. 16, 743-747 (1998). ...
Instead, plasma cells are identified through flow cytometry by their additional expression of CD138, CD78, the Interleukin-6 ... Plasma cells, also called plasma B cells, plasmocytes, plasmacytes, or effector B cells, are white blood cells that secrete ... The most immature blood cell that is considered of plasma cell lineage is the plasmablast.[3] Plasmablasts secrete more ... They are transported by the blood plasma and the lymphatic system. Plasma cells originate in the bone marrow; B cells ...
Many modern molecular tests such as flow cytometry, polymerase chain reaction (PCR), immunohistochemistry, cytogenetics, gene ... blood flow, or other), and a radionuclide (usually either a gamma-emitter or a positron emitter). There is a degree of overlap ... After examination for signs and interviewing for symptoms, the doctor may order medical tests (e.g. blood tests), take a biopsy ... "Chairman's Reflections: Traditional Medicine Among Gulf Arabs, Part II: Blood-letting". Heart Views. 5 (2): 74-85 [80]. 2004. ...
Flow-FISH[edit]. Flow-FISH uses flow cytometry to perform FISH automatically using per-cell fluorescence measurements. ... Microfluidics-assisted FISH (MA-FISH) uses a microfluidic flow to increase DNA hybridization efficiency, decreasing expensive ...
... flow cytometry). Additional biologically active molecules (such as antibodies) may also be attached to fluorescein, allowing ... in forensics and serology to detect latent blood stains, and in dye tracing. Fluorescein has an absorption maximum at 494 nm ... Fluorescein is used as a rather conservative flow tracer in hydrological tracer tests to help in understanding of water flow of ... Fluorescein has often been used to track water movement in groundwater to study water flow and observe areas of contamination ...
An optimized method for routine HLA-B27 screening using flow cytometry. Cytometry, 1994; 18:21-29. K. Strauss, Insulin ... Enumeration of CD4+ T-cells in the peripheral blood of HIV-infected patients: interlaboratory study of the FACSCount system. ... A comparative study of DNA content as measured by flow cytometry and image analysis in 1864 specimens. Analytical Cellular ... Cytometry. 1999 October 15;38(5):231-7. V. Chernyshov, E. Vykhovanets, I. Slukvin, Y. Antipkin, A. Vasyuk, K. Strauss, Analysis ...
Flow cytometry. *Blood bank. *Microbiological culture. *Serology. This cell biology article is a stub. You can help Wikipedia ... a red blood cell) and the neutrophil (a type of white blood cell). The maturing metarubricyte (a stage in RBC maturation) will ...
... where there is usually detectable protein levels by flow cytometry. The majority of the mutations causing classic WAS are ... 97 (9): 2633-9. doi:10.1182/blood.v97.9.2633. PMID 11313252.. *^ a b She HY, Rockow S, Tang J, Nishimura R, Skolnik EY, Chen M ... blood coagulation. • positive regulation of Arp2/3 complex-mediated actin nucleation. • actin filament-based movement. • ...
Flow cytometry. *Blood bank. *Microbiological culture. *Serology. Retrieved from " ... Møller M; El Maghrabi R; Olesen N; Thomsen VØ (November 2004). "Safe inoculation of blood and bone marrow for liquid culture ...
Fluorescence can be measured by means of multi-well fluorometers or by flow cytometry methods. There are also enzymatic-based ... Effector cells are typically PBMCs (peripheral blood mononuclear cell), of which a small percentage are NK cells (Natural ... "Cytometry Part A. 79 (8): 603-612. doi:10.1002/cyto.a.21084. ISSN 1552-4930. PMC 3692008. PMID 21735545.. ...
Additional examination of the bone marrow by tests including flow cytometry and FISH are necessary to diagnose the specific ... White blood cells (WBC) can show a variety of proteins on the surface depending upon the type of WBC. Leukaemic cells often ... These proteins can be stained with fluorescent dye labeled antibodies and detected using flow cytometry. The limit of detection ... Immature white blood cells may be present in the patient, although they are not necessarily malignant cells. In most cases, a ...
The preferred method of immunophenotyping is through flow cytometry. In the malignant lymphoblasts of ALL, expression of ... A large number of white blood cells and lymphoblasts in the circulating blood can be suspicious for ALL because they indicate a ... Diagnosing ALL begins with a thorough medical history, physical examination, complete blood count, and blood smears. While many ... white blood cells, and platelets.[1] Diagnosis is typically based on blood tests and bone marrow examination.[3] ...
DNA microarray chip - some can do as many as a million blood tests at once ... flow of genetic material from transgenic organisms into wild strains) can be seen as applications and implications, ... biomarker detection from blood, antibody detection, glucose measurement, pH sensing, and genetic technology.[19] ...
Torous, DK (2000). "Enumeration of micronucleated reticulocytes in rat peripheral blood: a flow cytometric study". Mutat Res ( ... Hutter, KJ (1982). "Rapid detection of mutagen induced micronucleated erythrocytes by flow cytometry". Histochemistry (75(3)): ...
Flow cytometry. *Blood bank. *Microbiological culture. *Serology. Authority control *LCCN: sh85050556. Retrieved from "https:// ...
The ability of the viral protein hemagglutinin to bind red blood cells together into a detectable matrix may also be ... which takes a blood meal from a person suspected of having been infected. The bug is later inspected for growth of T. cruzi ... the course of the disease was closely followed by monitoring the composition of patient blood samples, even though the outcome ... there is great therapeutic and predictive benefit to identifying the virus and monitoring the virus levels within the blood of ...
A pathologist then examines the tumor and its stroma, perhaps utilizing staining, immunohistochemistry, flow cytometry, or ... Carcinoma metastasizes through both the lymph nodes and the blood. Mutation[edit]. Whole genome sequencing has established the ... The surrounding pale staining, discoid cells are red blood cells. Cytopathology specimen. Field stain. ... while Stage IV tumors have already metastasized through the blood to distant sites, tissues, or organs. ...
Flow cytometry. *Blood bank. *Microbiological culture. *Serology. Retrieved from " ...
The deadliest diseases in humans are coronary artery disease (blood flow obstruction), followed by cerebrovascular disease and ... A systemic disease is a disease that affects the entire body, such as influenza or high blood pressure.. Classifications[edit] ... "Blood pressure drugs for mild hypertension: Not proven to prevent heart attacks, strokes, or early death". Slate. Archived ...
Flow cytometry. *Blood bank. *Microbiological culture. *Serology. This cell biology article is a stub. You can help Wikipedia ...
Antibodies are used in flow cytometry to differentiate cell types by the proteins they express; different types of cell express ... doi:10.1182/blood-2011-11-394874. PMID 22234685.. *^ a b Market, Eleonora; Papavasiliou, F. Nina (October 2003). "V(D)J ... Blood isolated from these animals contains polyclonal antibodies-multiple antibodies that bind to the same antigen-in the serum ... "Blood Groups and Red Cell Antigens. NCBI Bethesda (MD): National Library of Medicine (US),.. ...
... phosphatidylserine exposure versus cell permeability by flow cytometry), cellular markers such as DNA fragmentation[70] (flow ... However, upon immunization with SRBC (sheep red blood cells), these mice demonstrated a deficiency in the maturation of an ... "A selective procedure for DNA extraction from apoptotic cells applicable for gel electrophoresis and flow cytometry". ... "Cytometry. 27 (1): 1-20. doi:10.1002/(sici)1097-0320(19970101)27:1,1::aid-cyto2,;2-l. PMID 9000580.. ...
Several techniques use ions created in a dedicated ion source injected into a flow tube or a drift tube: selected ion flow tube ... Pharmacokinetics is often studied using mass spectrometry because of the complex nature of the matrix (often blood or urine) ... A fast on-line analysis of deuterium content of water can be done using flowing afterglow mass spectrometry, FA-MS. Probably ...
Flow cytometry. *Blood bank. *Microbiological culture. *Serology. *v. *t. *e. Clinical and histological nomenclature for skin ...
The rate of mean blood flow depends on both blood pressure and the resistance to flow presented by the blood vessels. Mean ... Relation between blood flow velocity and total cross-section area in human Type of blood vessels Total cross-section area Blood ... Blood[edit]. Main article: Blood. Blood is a complex liquid. Blood is composed of plasma and formed elements. The plasma ... Blood resistance varies depending on blood viscosity and its plugged flow (or sheath flow since they are complementary across ...
Less fastidious, scalable, methods include the use of cytometers, while flow cytometry allows combining cell counts ('events') ... spread to other locations in the body via lymph or blood). Several key determinants of cell growth, like ploidy and the ...
Less fastidious, scalable, methods include the use of cytometers, while flow cytometry allows combining cell counts ('events') ... spread to other locations in the body via lymph or blood). ...
March 2007). "Clinical value of anti-Leishmania (Leishmania) chagasi IgG titers detected by flow cytometry to distinguish ... Cases in the United States have proven L. infantum transmission from dog to dog by direct contamination with blood and ... absence of Leishmania parasites in blood, skin and lymph nodes of vaccinated exposed dogs". Vaccine. 23 (40): 4805-10. doi: ...
Since dense granules have surface membrane proteins, the activation of CD63 and LAMP-2 can be observed with flow cytometry. ... Blood, 120(19), 4072-4081. doi:10.1182/blood-2012-04-420745 *^ a b c d e f g h McNicol, A., & Israels, S. J. (1999). Platelet ... Another way to detect the secretion of dense granules is through flow cytometry. ... recruits other platelets and helps play a major role in stoping the loss of blood at the injury. The calcium from a dense ...
Sperm sorting is an advanced technique that sorts sperm "in vitro" by flow cytometry. This shines a laser at the sperm to ... For prenatal sex discernment, a blood test can be taken from the mother for testing of small amounts of fetal DNA within it, ... Johnson, L. A.; Flook, J. P.; Look, M. V. (1987). "Flow cytometry of X and Y chromosome-bearing sperm for DNA using an improved ... Sperm sorting utilizes the technique of flow cytometry to analyze and 'sort' spermatozoa. During the early to mid-1980s, Dr. ...
The Flow Cytometry Core is a state-of-the-art facility to accommodate a range of cell sorting and analysis needs. A wide ... imaging flow cytometry, phoshoprotein analysis, and cell cycle analysis. ...
Schiødt, I., Knudsen, L., Jensen, L. et al. Flow cytometry comparison of CD34+ subsets in bone marrow and peripheral blood ... Flow cytometry comparison of CD34+ subsets in bone marrow and peripheral blood after priming with glycosylated or non- ... Flow cytometry comparison of CD34+ subsets in bone marrow and peripheral blood after priming with glycosylated or non- ... Reduced dose of lenograstim is as efficacious as standard dose of filgrastim for peripheral blood stem cell mobilization and ...
Calculate absolute cell counts from percentage values for flow cytometry analysis. *Identify blood cell types associated with ... Which of these white blood cell populations would have the MOST side scatter when analyzed using flow cytometry? ... What cell population is gated in this peripheral blood sample case analyzed by flow cytometry? ... She has 14 years of experience in molecular diagnostics and flow cytometry. She has been a presenter at ASM, CLEC, and AABB ...
Top : New Forum Archives (2009-): : Flow Cytometry. Protocol for flow cytometry on mononuclear cells from mouse blood - (Mar/26 ... I´m new to flow cytometry and are setting up a protocol for flow cytometry on mononuclear cells from mouse blood, but would ... How much blood is needed to get how much separated cells ?? How many mice are needed ?? How do I collect the blood ?? ... I´m in need of a protocol for collection of blood and staining of isolated cells. ...
... subsets was prospectively analyzed using monoclonal antibodies and flow cytometry. Brief cryopreservation did not significantly ... The effect of cryopreservation and long-term liquid nitrogen storage on peripheral blood mononuclear cell (PBMC) ... Cryopreservation and long-term liquid nitrogen storage of peripheral blood mononuclear cells for flow cytometry analysis ... Cryopreservation and long-term liquid nitrogen storage of peripheral blood mononuclear cells for flow cytometry analysis: ...
Flow Cytometry Diagnostics Market Forecasts by Market Segment. Market Shares of Major Suppliers of Flow Cytometry Instruments ... 2017 Germany Diagnostics Market: Blood Banking, Cancer, Coagulation, Clinical Chemistry, Flow Cytometry, Hematology, ... ... Test Your Knowledge on Blood Groups. Blood groups are of four important types determined by the presence or absence of specific ...
... Asosingh, Kewal asosink at Thu Jul 16 12:34:06 EDT 2009 *Previous ... Does anyone has experience with phospho-flow of peripheral blood samples? We would like to know how long after the blood draw ... More information about the Cytometry mailing list. ... Next message: [Cytometry] Sorting Lamprey Cells * Messages ... message: [Cytometry] Sorting samples containing C. albicans * ... Next message: [Cytometry] Sorting Lamprey Cells * Messages ...
... and intermediate-1-risk myelodysplastic syndromes by flow cytometry. Blood, 111(3), 1067-1077. Accessed September 21, 2019. ... All flow cytometry data of patients with MDS and controls were translated into a numerical MDS flow-score according to the flow ... Flow cytometry data were translated into a numerical MDS flow-score. Flow-scores increased significantly from RA with or ... flow cytometry may add significantly. This is of particular interest since it suggests that flow cytometry may reclassify ...
... red blood cells (RBCs), and white blood cells (WBCs) from lysed whole blood and Ficoll preparations. Because the Countess™ ... Dead Cell Stains in Flow Cytometry: A comprehensive analysis. (Also download a Comprehensive Survey of Dead Cell Stains!) ... These cells can then be fixed with formaldehyde for subsequent analysis by flow cytometry. The LIVE/DEAD® Fixable Dead Cell ... Invitrogen now offers a broad range of antibodies with mouse reactivity for a variety of flow cytometry research applications ...
Intelligent whole-blood imaging flow cytometry for simple, rapid, and cost-effective drug-susceptibility testing of leukemia† ... Intelligent whole-blood imaging flow cytometry for simple, rapid, and cost-effective drug-susceptibility testing of leukemia H ... in whole blood within 24 hours by simply flowing a drug-treated whole blood sample as little as 500 μL into the imaging flow ... The method is based on an extreme-throughput (,1 million cells per second), label-free, whole-blood imaging flow cytometer with ...
The flow cytometry data presented in this article have been submitted to the ImmPort database under accession number SDY820. ... To Assess Technical and Biological Variability of Cell Population Frequencies in Human Peripheral Blood by Flow Cytometry. ... Thus, our study provides a universal workflow to establish and evaluate any flow cytometry panel in systems immunology studies. ... To Assess Technical and Biological Variability of Cell Population Frequencies in Human Peripheral Blood by Flow Cytometry ...
Flow Cytometry and Supravital Dyes); Approved Guideline-Second Edition. . Nonmember Price: $130.00 ... Methods for Reticulocyte Counting (Automated Blood Cell Counters, Flow Cytometry and Supravital Dyes), 2nd Edition. ... It includes methods for determining the trueness and precision of the reticulocyte flow cytometry instrument and a recommended ... This document provides guidance for the performance of reticulocyte counting by flow cytometry. ...
2016 France Diagnostics Market: Blood Banking, Cancer, Coagulation, Flow Cytometry, Hematology, Immunodiagnostics, Microbiology ... "2016 France Diagnostics Market: Blood Banking, Cancer, Coagulation, Flow Cytometry, Hematology, Immunodiagnostics, Microbiology ... 2020 Italy Hospital Hematology and Flow Cytometry Markets: Supplier Shares, Sales Segment Forecasts and Strategic Profiles of ... Blood Banking, Cancer, Coagulation, Flow Cytometry, Hematology, ... ...
Flow cytometry and mass spectrometry are widely used analytical tools in cancer research. Flow cytometry is known for cell ... The combination of flow cytometry to isolate specific cell subtypes from biological fluids such as blood and mass spectrometry ... Abstract 5318: LC-MS/MS quantification of proteins CD47 and SIRPA in cell subtypes selected by flow cytometry from blood. ... LC-MS/MS quantification of proteins CD47 and SIRPA in cell subtypes selected by flow cytometry from blood. [abstract]. In: ...
Hematology and Flow Cytometry - Immunoprotein. - Infectious Diseases. - Molecular Diagnostics. - Therapeutic Drug Monitoring ... Flow Cytometry, Hematology, Immunoproteins, Infectious Diseases, Molecular Diagnostics, TDM *January 2017 ... Blood Banking. - Cancer Diagnostics. - Chemistry - Coagulation. - Drugs of Abuse. - Endocrine Function. - ... 2017 Middle East Diagnostics Market Forecasts: Blood Banking, Cancer Diagnostics, Clinical Chemistry, Coagulation, Drugs of ...
Sequestration of flow cytometry to identify peripheral blood mononuclear cell (PBMC). * Post author By exposed ... Sequestration of flow cytometry to identify peripheral blood mononuclear cell (PBMC) profiles that are associated with PAM and ... the knowledge we have of such aspects is derived from examination of placental tissues at delivery and/or of peripheral blood, ... giemsa-stained thick and thin blood smears. All women received two standard curative treatment doses, spaced at least 1 month ...
Book IHC Marker CD 117 Flow Cytometry Blood @Home at Best Prices at the slot of your choice. View details of test: When to take ... Also known as CD 117 Count Flow Cytometry Blood, CD 117 Count Flow Cytometry, CD 117 Flow Cytometry, IHC Marker CD 117 Flow ... IHC Marker CD 117 Flow Cytometry Blood. Also known as Cd 117 Flow Cytometry Blood ... No special preparation is needed for IHC Marker CD 117 Flow Cytometry Blood. Inform your doctor if you are on any medications ...
analyzed by flow cytometry. Flow cytometry Analysis of the samples was carried out on a FACSVantage flow cytometer (BD ... aggregates in blood down to attomolar concentrations by flow cytometry. Results and discussion Detection of synthetic prion ... flow cytometry in blood from cattle affected with bovine spongiform encephalopathy Lothar Trieschmann1, Alexander Navarrete ... Subject: Ultra-sensitive detection of prion protein fibrils by flow cytometry in blood from cattle affected with bovine ...
Hematology and Flow Cytometry Markets. Hematology and Flow Cytometry Test Volume Forecasts. Hematology and Flow Cytometry ... Flow Cytometry Diagnostics Market Forecasts by Market Segment. Market Shares of Major Suppliers of Flow Cytometry Instruments ... Flow Cytometry Diagnostics Market Forecasts by Market Segment. Market Shares of Major Suppliers of Flow Cytometry Instruments. ... Blood Banking - Cancer - Chemistry. - Coagulation. - Drugs of Abuse. - Endocrine - Hematology and Flow Cytometry. - ...
... been developed for optimal lysis of red blood cells from human peripheral blood for in vitro diagnostic use in flow cytometry. ... whole blood samples were lysed with Red Blood Cell Lysis Solution followed by a washing step and analyzed by flow cytometry ... Red Blood Cell Lysis Solution (10×) has been developed for optimal lysis of red blood cells from human peripheral blood for ... For analysis, cell debris was excluded based on scatter signals and white blood cells were identified by CD45 fluorescence. ...
Flow Cytometry, Hematology, Immunoproteins, Infectious Diseases, Molecular Diagnostics, TDM report by Venture Planning Group. ... 40 Pages Report] Check for Discount on Czech Republic Diagnostics Market Forecasts: Blood Banking, Cancer Diagnostics, Clinical ... Hematology and Flow Cytometry Markets. Table 25: Hematology and Flow Cytometry Specimen Volume Forecast. Table 26: Hematology ... Blood Banking *Cancer *Chemistry *Coagulation *Drugs of Abuse *Endocrine *Hematology and Flow Cytometry *Immunoprotein * ...
Flow Cytometry, Hematology, Immunodiagnostics, Infectious Diseases, Microbiology, Molecular Diagnostics - Shares, Competitive ... Blood Banking Cancer Chemistry. Coagulation. Drugs of Abuse. Endocrine Hematology and Flow Cytometry. Immunoprotein Infectious ... 2017 Germany Diagnostics Market: Blood Banking, Cancer, Coagulation, Clinical Chemistry, Flow Cytometry, Hematology, ... 2017 Germany Diagnostics Market: Blood Banking, Cancer, Coagulation, Clinical Chemistry, Flow Cytometry, Hematology, ...
Harmonization of light scatter and fluorescence flow cytometry profiles obtained after staining peripheral blood leucocytes for ... Harmonization of light scatter and fluorescence flow cytometry profiles obtained after staining peripheral blood leucocytes for ... Automation, Cell fixation, Eigenvectors, Flow cytometry, Immunofluorescence, Intracellular proteins, Light scatter Persistent ... Cytometry Part B - Clinical Cytometry, 78(1), 11-20. doi:10.1002/cyto.b.20486 ...
Flow Cytometry, Hematology, Immunoproteins, Infectious Diseases, Molecular Diagnostics, - Market research report and industry ... Blood Banking, Cancer Diagnostics, Clinical Chemistry, Coagulation, Drugs of Abuse, Endocrine Function, ... Blood Banking Cancer Chemistry. Coagulation. Drugs of Abuse. Endocrine Hematology and Flow Cytometry. Immunoprotein Infectious ... Flow Cytometry, Hematology, Immunoproteins, Infectious Diseases, Molecular Diagnostics, TDM. This new report from ...
Flow cytometry is an important tool for the diagnosis and classification of leukemia. While many leukocyte markers have been ... Flow cytometry is an important tool for the diagnosis and classification of leukemia. While many leukocyte markers have been ... Multiparameter White Blood Cell Panel Designed For The Immunophenotyping Of Normal And Malignant Leukocytes By Flow Cytometry. ... A 19-antibody white blood cell panel was developed using the ZE5™ Cell Analyzer from Bio-Rad Laboratories. With up to five ...
An optimized multiplex flow cytometry protocol for the analysis of intracellular signaling in peripheral blood mononuclear ... Phosphoflow cytometry is increasingly being used as a tool for the discovery of biomarkers used in the treatment and monitoring ... Three different blood collection tubes (lithium-heparin tubes, CPT with sodium citrate and CPT with sodium heparin) were ... we provide a protocol outlining an 8 color assay developed for the study of intracellular signaling in peripheral blood ...
Fetomaternal Bleed,Flow Cytometry,B. Aliases Lists additional common names for a test, as an aid in searching. Fetal Hemoglobin ... Fetal Maternal Erythrocyte Distribution, Blood. Fetal Red Cells for Fetal Maternal Erythrocyte Distribution. Fetal-Maternal ...
Test ID CD40 B-Cell CD40 Expression by Flow Cytometry, Blood Useful For. Evaluating patients for hyper-IgM type 3 (HIGM3) ... Whole Blood EDTA Shipping Instructions. Specimens are required to be received in the laboratory weekdays and by 4 p.m. on ... CD40 by Flow, QL, B Specimen Type. ...
View the review history for Influence of rimonabant treatment on peripheral blood mononuclear cells; flow cytometry analysis ... Influence of rimonabant treatment on peripheral blood mononuclear cells; flow cytometry analysis and gene expression profiling ... In this work "Influence of rimonabant treatment on peripheral blood mononuclear cells; flow cytometry analysis and gene ... Material and methods, Flow cytometry, line 80 " The phenotypes of cells…was" it should be " the phenotype".. Results, line 141 ...
... to assess technical and biological variability of cell population frequencies in human peripheral blood by flow cytometry. In: ... to assess technical and biological variability of cell population frequencies in human peripheral blood by flow cytometry. ... to assess technical and biological variability of cell population frequencies in human peripheral blood by flow cytometry, ... to assess technical and biological variability of cell population frequencies in human peripheral blood by flow cytometry. ...
  • A 19-antibody white blood cell panel was developed using the ZE5™ Cell Analyzer from Bio-Rad Laboratories. (
  • This new smaller size is an ideal option for researchers conducting pilot flow cytometry experiments or trial testing of an antibody. (
  • Beckman Coulter Life Sciences has taken the detection of rare, abnormal events in blood disorders a step forward, with the introduction of advanced and highly discriminative antibody panels. (
  • DuraClone RE antibody panels improve detection of rare, abnormal events in blood disorders. (
  • Retrieved on December 05, 2020 from (
  • Blood samples were thawed as described above, resuspended in RPMI 1640 containing 10% FCS to 1 × 10 6 cells/ml, and then plated into replicate uncoated and antibody-coated wells. (
  • Its main cause is auto-antibody mediated rapid destruction of Red Blood Cells (RBCs). (
  • Lynen R, Bernbeck B, Legler T, Köhler M (1997) Studies on the sensitivity of IgG red cell antibody determination using the gel configuration test and flow cytometry. (
  • A flow cytometry test for intracellular Btk in monocytes using an anti-Btk monoclonal antibody was developed by Futatani et al, which was used to evaluate both XLA patients and carriers. (
  • We studied peripheral blood (PB) from 161 cases of HCL using two-color direct immunofluorescence flow cytometry and an extended panel of antibody combinations. (
  • We conclude that two-color flow cytometry with specific antibody combinations is an efficacious method for characterization and sensitive detection of hairy cells in PB. (
  • Remove any unbound antibody by washing the cells in 2 mL Flow Cytometry Staining Buffer. (
  • Dilute the secondary antibody in Flow Cytometry Staining Buffer, starting with the suggested concentration in the product datasheet. (
  • Samples were tested at different time points to detect microparticles and determine the haemolysis rate (HR%). Microparticles were identified by flow cytometry combining carboxyfluorescein diacetate succinimidyl ester (CFSE) dye, annexin V and anti-glycophorin A antibody. (
  • Among immunofluorescence assays using flow cytometry for platelet antibodies , a direct method ( platelet -associated IgG tesing, PAIgG) and an indirect method (antiplatelet antibody testing, APA) are used with fresh platelets . (
  • The antibody profile serves as a warning sign for animals that require a second blood transfusion , which is only advisable from compatible donor dogs . (
  • On post-transfusion days 7, 14, 21 and 28, 45% and 27% of the animals tested positive for the anti-DEA 1 antibody, through crossmatching and flow cytometry , respectively. (
  • One μL of infected blood was washed, fixed, stained with a Plasmodium pan-specific anti-PfBiP antibody conjugated with Alexa Fluor 660, and analysed by FC. (
  • The objective of the study was to develop a strategy for rapid antibody-based staining to identify P. vivax -infected red blood cells as well as determine P. vivax stages of development. (
  • In addition to HIV antibody testing in NHANES, whole-blood samples were collected and stored for future CD4 testing once the HIV status of the sample was known. (
  • Participants aged 18-49 years who did not refuse the HIV antibody test and had a blood sample available for CD4+ testing were considered eligible for testing. (
  • Whole EDTA anti-coagulated blood stored at -70°C from the 31 HIV antibody-positive participants from NHANES 1999-2004 and 34 age-matched controls were used. (
  • A potentially good antibody for flow cytometry staining of TNFRSF26. (
  • Every time someone gets his or her blood drawn, whether for an annual physical, or to follow up on a treatment, that tube of blood gets sent to a lab, just downstairs or maybe a few hundred miles away, where flow-based hematology analyzers generate a complete blood count (CBC), which includes red blood cells, white blood cells and platelets, in less than a minute per sample. (
  • However the high costs and the trained technical specialist required the flow cytometry has a wide variety of applications in the veterinary hematology including hematopoietic stem cells erythrocyte, leukocyte and platelet evaluation. (
  • Scientific assays about flow cytometry evaluation of the stem cell and lymphocyte subsets of dogs have been performed successfully by veterinary hematology scientists in the Universidade Estadual Paulista (UNESP), Campus Jaboticabal. (
  • I´m new to flow cytometry and are setting up a protocol for flow cytometry on mononuclear cells from mouse blood, but would like some input from people with experience. (
  • I have bought a kit from Cedarlane (Lympholyte®-Mammal Cell Separation Media (Isolation of lymphocytes from Mammalian peripheral blood), sterile liquid) to isolate the mononuclear cells. (
  • The effect of cryopreservation and long-term liquid nitrogen storage on peripheral blood mononuclear cell (PBMC) subsets was prospectively analyzed using monoclonal antibodies and flow cytometry. (
  • Sequestration of flow cytometry to identify peripheral blood mononuclear cell (PBMC) profiles that are associated with PAM and anaemia, determining the phenotypic composition and activation status of PBMC in selected sub-groups with and without PAM both at inclusion and at delivery in a total of 302 women. (
  • Within the overall framework of the STOPPAM project, the study's primary objective was thus to evaluate the impact of pregnancy-associated malaria (PAM) on the phenotypic composition and activation status of peripheral blood mononuclear cells (PBMC), and to attempt to identify PBMC profiles that are associated with particular outcomes e.g. maternal anaemia, in order to better understand the pathogenesis of PAM. (
  • Further, we provide a protocol outlining an 8 color assay developed for the study of intracellular signaling in peripheral blood mononuclear cells. (
  • We used global gene expression profiling of peripheral blood mononuclear cells to identify distinct patterns of gene expression that distinguish most SLE patients from healthy controls. (
  • In this study we explore the hypothesis that gene expression profiling of peripheral blood mononuclear cells (PBMCs), which are comprised of monocytes/macrophages, B and T lymphocytes, and natural killer cells, may provide new insights into the pathophysiology of SLE. (
  • EPCs were isolated and expanded on day 7 of culture from cord blood mononuclear cells. (
  • METHODS Peripheral blood mononuclear cells from 65 subjects (25 HLA-B27 positive patients with active AS, 18 healthy HLA-B27 positive controls, and 22 healthy HLA-B27 negative controls) were stimulated with phorbol myristate acetate/ionomycin for six hours, surface stained for CD3 and CD8, intracellularly stained for the cytokines IFNγ, TNFα, IL4, and IL10, and analysed by flow cytometry. (
  • I´m in need of a protocol for collection of blood and staining of isolated cells. (
  • Characterization of normal bone marrow cells by flow cytometry is well established. (
  • These cells can then be fixed with formaldehyde for subsequent analysis by flow cytometry. (
  • 1 million cells per second), label-free, whole-blood imaging flow cytometer with a deep convolutional autoencoder, enabling image-based identification of the drug susceptibility of every single white blood cell in whole blood within 24 hours by simply flowing a drug-treated whole blood sample as little as 500 μL into the imaging flow cytometer without labeling. (
  • Our results show that the method accurately evaluates the drug susceptibility of white blood cells from untreated patients with acute lymphoblastic leukemia. (
  • The combination of flow cytometry to isolate specific cell subtypes from biological fluids such as blood and mass spectrometry to quantify proteins contained in the selected cells provides a new approach to studying protein expression in specific cell subtypes. (
  • We have used this combination to investigate the normal levels of CD47 and SIRPA proteins in CB8+ T-cells, CD4+ T-cells, CD14+ monocytes, CD33+ myeloid cells and CD56+ NK cells isolated from blood specimens as a first step toward better understanding of how the CD47/SIRPA protein levels in these cells are affected by cancer and cancer treatment. (
  • For analysis, cell debris was excluded based on scatter signals and white blood cells were identified by CD45 fluorescence. (
  • Lior Golan, Daniella Yeheskely-Hayon, Limor Minai, Eldad J Dann, and Dvir Yelin, "Noninvasive imaging of flowing blood cells using label-free spectrally encoded flow cytometry," Biomed. (
  • Label-free characterization of white blood cells using fluorescence lifetime imaging and. (
  • CD-Chex® Plus controls for immunophenotyping by flow cytometry closely resemble patient samples with the inclusion of both red and white cells stabilised in a way which preserves the antigen sites. (
  • Greater than 15 mL of fetal red blood cells (RBC) (30 mL of fetal whole blood) is consistent with significant fetomaternal hemorrhage (FMH). (
  • The recently optimized method of intracellular cytokine detection in specific cells by flow cytometry circumvents these limitations ( 10 ) and has great potential for biomonitoring of immune function in human populations ( 11 ). (
  • Flow cytometric detection of intracellular cytokines is a functional assay that measures the ability of specific immune cells to express type 1 and type 2 cytokines after polyclonal stimulation with mitogens ( 8 , 10 , 12 ). (
  • Research of rare abnormal cells associated with blood disorders is powerful, yet challenging to perform," explained MarioKoksch, Vice President and General Manager of Beckman Coulter's Cytometry Business Unit. (
  • Whole blood cryopreservation is a cost-effective approach to large-scale storage of viable cells in epidemiological studies. (
  • Cells were analyzed on a Coulter XL flow cytometer. (
  • 1 Circulating blood monocytes develop from bone marrow progenitor cells and enter tissues to further differentiate, mainly into resident tissue macrophages and dendritic cells. (
  • The later steps of these pathways can be reproduced ex vivo by incubating blood monocytes with macrophage-colony-stimulating factor (M-CSF) to generate macrophages or with granulocyte macrophage-colony-stimulating factor (GM-CSF) and IL-4 to generate dendritic cells. (
  • Half-life studies using blood from patients with pulmonary tuberculosis and THP1 cells exposed to Myobacterium tuberculosis in vitro showed p38 MAPK-independent stabilization of mRNAs encoding hsTLR1 and TLR1. (
  • Taking blood into paraformaldehyde, or fixing the cells with paraformaldehyde as soon as possible after withdrawal, has been employed to prevent platelet activation in vitro, but paraformaldehyde-fixed platelets cannot be further used in functional studies. (
  • Arthritis scores and joint swelling were significantly lower than the vehicle control, accompanied by decreases in blood levels of C-reactive protein, alkaline phosphatase, and natural killer, B, and T cells. (
  • Because endothelial progenitor cells (EPCs) have been identified in adult human peripheral blood, many studies have shown that transplantation of EPCs improves circulation to ischemic tissues. (
  • 9 ) first demonstrated the existence of circulating endothelial progenitor cells (EPCs) in adult peripheral blood, the concept of adult vasculogenesis has been developed. (
  • Van der Meulen F, De Bruin H, Goosen P, Bruynes E, Joustra-Maas C, Telkamp H et al (1980) Quantitative aspects of the destruction of red cells sensitized with IgG1 autoantibodies: an application of flow cytofluorometry. (
  • In: Nance SJ (ed) Immune destruction of red blood cells. (
  • Aspects of the flow cytometric analysis of red blood cells. (
  • XLA is caused by variants in the Bruton tyrosine kinase gene ( BTK ),(1) which results in a profound block in B-cell development within the bone marrow and a significant reduction, or complete absence, of mature B cells in peripheral blood. (
  • Flow cytometry, by contrast, provides only a handful of measured parameters on each cell, but at rates of tens of thousands of cells per second. (
  • Most flow cytometers work by funneling cells from a liquid sample into a narrow stream so they're in single file, and passing them one by one at high speed through one or more interrogating laser beams (see Figure 1). (
  • Cells ( red ) flow one by one past the beam from a laser ( gray ), and the resulting light signals ( various colors ) are spectrally separated by filters ( cyan ) and detected by photodiodes and photomultipliers ( pink ). (
  • Flow cytometry can distinguish those cells from all other blood cells - quickly and reliably. (
  • An elevated concentration of fibrinogen in blood is a significant risk factor during many pathological diseases, as it leads to an increase in red blood cells (RBC) aggregation, resulting in hemorheological disorders. (
  • Leukemias are genetically and biologically heterogeneous hematopoietic malignancies characterized by the expansion of transformed cells which interferes with the proper differentiation and maturation of all blood lineages. (
  • The percentages of interferon γ (IFNγ)- and interleukin (IL)4-producing Th cells in the PB of 20 patients with active untreated AOSD, 20 patients with active rheumatoid arthritis (RA), and 20 healthy controls were determined by intracellular staining and flow cytometry. (
  • Phenotyping suspended cells based on antigens present on the membrane is perhaps the most common use for flow cytometry. (
  • Sample preparation: For staining peripheral blood cells, whole blood should be collected in evacuated tubes containing EDTA or heparin as the anticoagulant. (
  • Resuspend the cells by adding 2 mL of Flow Cytometry Staining Buffer. (
  • Lysis of Red Blood Cells: Add 2 mL of 1X Human (Catalog # FC002 ) or Mouse (Catalog # FC003 ) Lyse Buffer to each tube, vortex, and incubate in the dark at room temperature for 10 minutes. (
  • Centrifuge and wash cells in Flow Cytometry Staining Buffer as described in step 4 above. (
  • Resuspend the cells in 200 - 400 μL of Flow Cytometry Staining Buffer for final flow cytometric analysis. (
  • Increased HbF production correlates with a higher number of HbF-containing red blood cells (RBCs) called F-cells. (
  • Comparison of in vitro sickling among blood specimens from sickle mouse models and from patients with different HbF levels has provided compelling evidence that increasing the percentage of circulating F-cells is associated with improvement of hemolytic biomarkers. (
  • RBCs were then labeled for HbF using a standard protocol for F-cell quantitation and a minimum of 20,000 cells were analyzed by imaging flow cytometry (ImageStream X Mk II, Amnis Corporation), allowing the correlation between shape change and intensity of HbF expression for each RBC. (
  • During the last decades, extended characterizations were performed of human full-term cord blood (hTCB) cells, but little information is available on human early preterm cord blood (hEPCB) hematopoietic stem cells (HSCs). (
  • CD-Chex Plus control cells mimic whole blood samples by possessing surface antigens detectable with fluorescent monoclonal antibodies by flow cytometry. (
  • Summary of flow cytometry analysis (n = 7) of percentage of phosphorylated NF-κB (A) and phosphorylated STAT3 (B) activation (versus untreated) from B-cells, T-lymphocytes and monocytes/macrophages. (
  • Jonas Gienger, Hermann Gross, Volker Ost, Markus Bär, and Jörg Neukammer, "Assessment of deformation of human red blood cells in flow cytometry: measurement and simulation of bimodal forward scatter distributions," Biomed. (
  • Light scattering by single cells is widely applied for flow cytometric differentiation of cells. (
  • However, even for human red blood cells (RBCs), which can be modeled as homogeneous dielectric particles, the potential of light scattering is not yet fully exploited. (
  • The percentages of P. vivax -infected cells determined by the FC method and manually by microscopic examination of Giemsa-stained thick blood smears were positively correlated by Spearman's rank correlation coefficient ( R 2 = 0.93843) from 0.001 to 1.00% P. vivax -infected reticulocytes. (
  • Diagnosis of malaria parasites in field isolates typically relies on light microscopic detection of Plasmodium -infected blood cells in Giemsa-stained smears. (
  • Many of these methods use nucleic acid staining to detect parasite-infected cells, since nucleated white blood cells (WBCs) are removed for culture and mature erythrocytes retain virtually no nucleic acid. (
  • This has led to investigation of alternative staining strategies to identify the parasite-infected blood cells from field isolates. (
  • Using immunophenotyping, HIV-positive blood samples and age-matched controls were tested for the proportion of lymphocytes that are T cells, B cells, natural killer (NK) cells, CD4+ T cells (helper T cells), and CD8+ T cells (suppressor/inducer T cells). (
  • Autoantibodies targeting different antigenic determinants on red blood cells (RBCs) and platelets are assumed to cause isolated episodes of hemolytic anemia and thrombocytopenia, respectively. (
  • Teachey et al screened 12 children by using flow cytometry for CD4/CD8 (double-negative) T cells and using the definitive test for ALPS (ie, defective in vitro Fas-mediated apoptosis). (
  • Flow cytometry is an emergent method in veterinary medicine which provides the identification and quantification of the suspension cells. (
  • They looked to previous research as a starting point, back in 2013, an international team of researchers across institutions in Germany, France, and the US, discovered that particular profiling panels (sets of characteristics that are detected and measured) could be used with flow cytometers to observe immune cells involved with transplantation. (
  • The team, therefore, aimed to establish a standardized method of flow cytometry to measure the immune response following a transplant by monitoring the immune cells existing within blood samples. (
  • Previously described in the research conducted in 2013, the team used the profiling panels as a starting point and developed their consistent method of using flow cytometry to measure immune-cell-count and analyze subsets of immune cells found in the blood samples of transplant patients. (
  • Chronic lymphocytic leukemia (CLL) is cancer of a type of white blood cells called lymphocytes. (
  • Bone marrow is the soft tissue in the center of bones that helps form all blood cells. (
  • CLL causes a slow increase in a certain type of white blood cells called B lymphocytes, or B cells. (
  • Cancer cells spread through the blood and bone marrow. (
  • Flow cytometry test of the white blood cells. (
  • Spectrally encoded flow cytometry (SEFC) is a promising technique for noninvasive in vivo microscopy of blood cells. (
  • The new system employs interferometric Fourier-domain detection and a high-speed wavelength-swept source, allowing 100 kHz line rate, sufficient for sampling the rapidly flowing cells 80 μm below the tissue surface. (
  • When requesting a flow cytometry DNA histogram, select a block with a minimum of 20% malignant cells. (
  • Flow cytometry (FC) is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles. (
  • In this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument. (
  • The sample is focused to ideally flow one cell at a time through a laser beam, where the light scattered is characteristic to the cells and their components. (
  • Other instruments using flow cytometry include cell sorters which physically separate and thereby purify cells of interest based on their optical properties. (
  • The flow cell has a liquid stream (sheath fluid), which carries and aligns the cells so that they pass single file through the light beam for sensing. (
  • Dendritic cells that circulate in blood do not have all the typical features of their counterparts in tissue, i.e. they are less mature and have no dendrites. (
  • For ease of use and storage, the 25-test size flow cytometry antibodies are bottled in microcentrifuge-ready amber polypropylene copolymer vials. (
  • Lynen R, Krone O, Legler TJ, Köhler M, Mayr WR (2002) A newly developed gel centrifugation test for quantification of RBC-bound IgG antibodies and their subclasses IgG1 and IgG3: comparison with flow cytometry. (
  • This article focuses on determination of anti-DEA 1 antibodies using the flow cytometry technique in dogs that have undergone a transfusion using DEA 1-positive blood , compared to results obtained from crossmatching. (
  • Detecting antibodies using the flow cytometric technique has high specificity and sensitivity , while crossmatching methods are highly sensitive but manifest low specificity . (
  • NSH Flow Cytometry and Molecular Diagnostics Laboratories, under the direction of Irina Grigorieva, PhD, perform high-complexity clinical assays by utilizing innovative equipment and progressive technologies in their 4,600 square feet state-of-the-art facilities. (
  • Stroncek D, Njoroge J, Procter J, Childs R, Miller J (2003) A preliminary comparison of flow cytometry and tube agglutination assays in detecting red blood cell-associated C3d. (
  • This guideline addresses the diagnostic red blood cell (RBC) assays performed as fluorescence-based assays on a flow cytometry platform. (
  • Cytometry B Clin Cytom 2016 Sep 01;90(5):401-3. (
  • Minimal Residual Disease in Multiple Myeloma - test is developed based on European Myeloma Network (EMN 2013) and Consensus Guidelines of International Clinical Cytometry Society (ICCS 2016). (
  • The clinical severity of the disease can vary from intrauterine death to hematological abnormalities detected only if blood from an apparently healthy infant is subject to serologic testing. (
  • Our work in clinical research is focused on novel cellular and immune approaches to target blood cell disorders. (
  • Today, flow cytometers are ubiquitous in hospitals, clinical laboratories, cell biology departments, marine biology laboratories, biomedical research institutions and development labs of pharmaceutical companies. (
  • There is growing evidence of the clinical importance of microparticles and their role in blood transfusion-related side effects and pathogen transmission. (
  • Therefore, analysis of clinical isolates and the preference of P. vivax for reticulocytes, which still retain abundant amounts of nucleic acids in the cytosol, complicates the use of the nucleic acid stain-based flow cytometry (FC) methods. (
  • The important clinical application of the flow cytometry is the CD34 + quantification to hematopoietic reconstitution after stem cell transplant and lymphocyte subsets to evaluate the immune reply to tissue or organ transplant and animals diseases. (
  • Flow cytometry is routinely used in basic research, clinical practice, and clinical trials. (
  • Early flow cytometers were, in general, experimental devices, but technological advances have enabled widespread applications for use in a variety of both clinical and research purposes. (
  • List the appropriate specimen types used for flow cytometric analysis. (
  • Patients in progression to advanced MDS or acute myeloid leukemia had a significantly higher flow-score compared with non-transfusion-dependent patients. (
  • In addition, flow cytometry identified patients at risk for transfusion dependency and/or progressive disease independent of known risk groups, which might have impact on treatment decisions and the prognostic scoring system in the near future. (
  • Following blood transfusion , animals that did not present as positive through crossmatching or flow cytometry were considered different from all other DEA 1-positive blood groups . (
  • Pilot studies showed that lymphocytes from blood collected in acid-citrate-dextrose maintained viability equal or superior to other anticoagulants, especially at time points beyond 48 h, and that cryopreserved samples yielded viable lymphocytes. (
  • HIV specifically targets CD4-positive T-lymphocytes (a kind of white blood cell), depleting them and making the patient vulnerable to infection. (
  • Enumeration of CD4+ lymphocytes in HIV-positive participants and age-matched controls was performed on cryopreserved whole blood using the method reported by Fiebig et. (
  • The aim of this study was to use RNA sequencing to analyse the effect of REV on immunity and cell proliferation in chicken lymphocytes from peripheral blood in vitro . (
  • These results indicate that REV could affect lymphocytes from peripheral blood by inhibit the cell proliferation and the immune system. (
  • The present study investigated the effect of REV on chicken blood lymphocytes. (
  • Blood was collected from the wing vein, using citric acid dextrose as an anticoagulant for subsequent lymphocytes derived. (
  • Interpret basic flow cytometer instrument analysis printouts. (
  • A large vapor bubble in a 250-µm flow cytometer microchannel caused by absorption of high-power green light by fluorescein dye. (
  • Bubbles large and small can create mesmerizing interactions with a laser beam, however they are very disruptive to precision cell measurements, and flow cytometer designers go to great lengths to prevent them. (
  • Flow cytometer laser and filter requirements are also listed. (
  • The first label-free high-frequency impedance flow cytometer based on a patented microfluidic "lab-on-chip", Ampha Z30, was introduced by Amphasys (2012). (
  • At the 5th American Engineering Foundation Conference on Automated Cytology in Pensacola (Florida) in 1976 - eight years after the introduction of the first fluorescence-based flow cytometer (1968) - it was agreed to commonly use the name "flow cytometry", a term that quickly became popular. (
  • A flow cytometer is similar to a microscope, except that, instead of producing an image of the cell, flow cytometry offers high-throughput, automated quantification of specified optical parameters on a cell-by-cell basis. (
  • A flow cytometer has five main components: a flow cell, a measuring system, a detector, an amplification system, and a computer for analysis of the signals. (
  • Acquisition is mediated by a computer physically connected to the flow cytometer, and the software which handles the digital interface with the cytometer. (
  • This test is for the detection of fetal bleed, it should not be used to detect the hereditary persistence of fetal hemoglobin (see HPFH / Hemoglobin F, Red Cell Distribution, Blood) or to detect fetal maternal hemorrhage in a mother with hereditary persistence of fetal hemoglobin. (
  • Here, we report our validation results for flow cytometric detection of intracellular T-helper 1/T-helper 2 (Th1/Th2) cytokines using 500 μL of whole blood obtained from children and adults. (
  • The detection of Th1/Th2 cytokine profiles by flow cytometry is a practical and mechanistically relevant assay because dysregulated cytokine production has been observed in many immune-mediated disorders, including cancer. (
  • These results show that flow cytometric detection of CD4, Th1, and Th2 markers using whole blood is reproducible and that these biomarkers can be effectively used in human population studies that involve transported samples, delayed processing and analysis, and limited blood volumes. (
  • We evaluated CMV antigen detection by flow cytometry in blood samples collected before and after transplantation in 56 serially collected blood samples from 17 HSCT patients and CMV protein expression was compared to CMV isolation. (
  • However, CMV L protein detection by flow cytometry correlated with virus isolation in serially collected blood samples. (
  • There was a 100% correlation (8/8) between the lack of CMV antigen detection by flow cytometry and the failure to isolate infectious virus. (
  • This study indicates that flow cytometric identification of CMV antigenemia correlates with isolation of CMV in HSCT patients and may be a predictive test for the rapid detection of CMV in the blood. (
  • Blood samples of suspected patients of AIHA were tested by both Gel Card Test and by Flow-cytometry for detection of RBC bound IgG. (
  • A total of 50 patients with suspected diagnosis of AIHA were studied by flow-cytometry as well as by Gel card test for detection of RBC bound IgG. (
  • Chaudhary R, Das SS, Gupta R, Khetan D (2006) Application of flow cytometry in detection of red cell bound IgG in Coombs negative AIHA. (
  • Wang Z, Shi J, Zhou Y, Ruan C (2001) Detection of red blood cell-bound immunoglobulin G by flow cytometry and its application in the diagnosis of autoimmune hemolytic anemia. (
  • Detection of TM4SF1/L6 in A549 Human Cell Line by Flow Cytometry. (
  • These features complicate detection and quantification by flow cytometry (FC) using standard nucleic acid-based staining methods. (
  • An optical detection system for flow cytometry that uses two or more light sources positioned laterally at different distances from the central axis of the flow stream for providing light through different parts of the flow stream. (
  • Uses for flow cytometry include: Cell counting Cell sorting Determining cell characteristics and function Detecting microorganisms Biomarker detection Protein engineering detection Diagnosis of health disorders such as blood cancers A flow cytometry analyzer is an instrument that provides quantifiable data from a sample. (
  • Methods and Results- EPCs were defined by the expression of CD34, CD133 and KDR, and quantified by flow cytometry in 127 diabetic patients with and without PAD. (
  • However, subset of 'inflammatory' monocytes in the blood also expresses CD16. (
  • M-CSF-driven differentiation of peripheral blood monocytes is one of the sources of tissue macrophages. (
  • In this study, we report on a universal workflow to evaluate both technical and biological variation in multiparameter flow cytometry, applied to the development of a 10-color panel to identify all major cell populations and T cell subsets in cryopreserved PBMC. (
  • Quantitation of peripheral blood leukocyte subsets has been shown to be useful in the evaluation of immunodeficiency, autoimmune, and oncologic disorders. (
  • Describe the steps involved in analyzing samples by flow cytometry. (
  • Does anyone has experience with phospho-flow of peripheral blood samples? (
  • Expression of the CD29, CD49b and CD31 adhesion molecules on the platelet surface was unaffected by storage in Diatube-H. The results suggest that Diatube-H may be a useful reagent for flow cytometric studies of platelets when the samples cannot be processed immediately. (
  • If using whole blood, samples should go through a red blood cell lysis step at this point using Flow Cytometry Human or Mouse Lysis Buffer. (
  • Peripheral venous blood samples were collected upon written consent from adult SCA patients with a wide range of HbF percentages (HbF range 2.0-26.9%) (n=15, nine on hydroxyurea treatment). (
  • In our study, we analyzed by flow cytometry 19 hEPCB and 17 hTCB samples. (
  • However, blood samples directly from malaria patients frequently have many contaminating WBCs and anaemia in chronic infections can enhance reticulocytaemia. (
  • The resultant method was tested, and researchers considered that it was easy to use and successful at producing consistent results across multiple blood samples. (
  • Further to this, the established method was of minimal invasiveness to patients, with just 1.5ml of blood required for viable samples. (
  • In flow cytometric analyses of in vivo platelet activation, results are often confounded by activation induced in vitro by the preparative procedures. (
  • It is particularly important therefore to prevent or retard platelet activation as soon as possible after withdrawal of the blood sample. (
  • In contrast to blood taken into EDTA, blood taken into Diatube-H vacutainer tubes could be stored at room temperature for up to 4 hr prior to paraformaldehyde fixation without significant in vitro platelet activation, as measured by CD62P, CD63 and modulation of GPIb and GPIIbIIIa surface expression. (
  • The assessment of platelet activation by whole-blood flow cytometry is a recent development and is potentially a much more specific and reproducible technique than traditional aggregation studies ( 17 ). (
  • The relatively elevated cost and complexity of traditional flow cytometers means that, generally, laboratories share them: Instruments are acquired by a central facility, and then allocated to users across a department, an entire hospital, or even from other institutions. (
  • It includes methods for determining the trueness and precision of the reticulocyte flow cytometry instrument and a recommended reference procedure. (
  • Three different blood collection tubes (lithium-heparin tubes, CPT with sodium citrate and CPT with sodium heparin) were evaluated, with PBMC isolated through lithium-heparin tubes/lymphoprep displaying reduced basal and increased stimulation induced phosphorylation compared to the other two methods. (
  • Methods Expression of seven platelet surface antigens (including P-selectin, activated GPIIb-IIIa and GPIb-IX), whole-blood platelet aggregation, platelet count and hematocrit were measured before and after arising in 17 normal volunteers. (
  • Methods and Results- CD14 dim CD16 + and CD14 + CD16 + frequencies were measured by flow cytometry in lean subjects, obese subjects before and after a hypocaloric diet or gastric surgery, and obese diabetic subjects before and after gastric surgery. (
  • Material and methods: RBC units (n=10) were stored under blood bank conditions for up to 42 days. (
  • She has 14 years of experience in molecular diagnostics and flow cytometry. (
  • The following protocol has been developed and optimized by R&D Systems Flow Cytometry Laboratory for the staining of membrane-associated proteins. (
  • High sensitivity flow cytometry of membrane vesicles. (
  • Cell size is indicated by forward scatter and granularity is indicated by side scatter in flow cytometry. (
  • Which of these white blood cell populations would have the MOST side scatter when analyzed using flow cytometry? (
  • Staining for intracellular markers with the Fix & Perm™ reagent is associated with variations in the scatter properties of leucocytes, limiting automated analysis of flow cytometry (FCM) data. (
  • Clever innovation and disciplined engineering are helping to create better, smaller and less expensive flow cytometers. (
  • Traditional flow cytometers (such as BD Biosciences' 525-lb LSR II) have long been just the opposite - bulky, lab-bound and complicated to operate. (
  • BD Biosciences offers flow cytometers, reagents, tools and a wide range of services to support the work of researchers and clinicians who understand disease and strive to improve care. (
  • Mack Fulwyler was the inventor of the forerunner to today's flow cytometers - particularly the cell sorter. (
  • Modern flow cytometers are able to analyze many thousands of particles per second, in "real time" and, if configured as cell sorters, can actively separate and isolate particles with specified optical properties at similar rates. (
  • Infection levels are often low and accurate determination of a parasitaemia requires the use of thick Giemsa-stained blood smears instead of thin blood smears used in acute infections or for monitoring in vitro cultures. (
  • These findings suggest that transplantation of EPCs from cord blood may be a useful treatment for diabetic neuropathy. (
  • We investigated TLR gene expression in fresh unstimulated blood and bronchoalveolar lavage from patients with pulmonary tuberculosis using a well-validated, real-time PCR. (
  • Conclusions These data demonstrate that the morning increase in platelet aggregation is not accompanied by expression of activation-dependent platelet surface receptors and suggest that the increase in whole-blood aggregation may be primarily due to the increases in catecholamine levels, platelet count and hemocon-centration. (
  • The purpose of this study was to determine if CMV proteins detected by flow cytometry could be a rapid and more quantitative way to monitor CMV infections and CMV antigenemia in HSCT patients. (
  • Isolate PBMCs from whole blood by Ficoll density centrifugation as per manufacturer's instructions. (
  • Soon after, flow cytometry instruments were developed, including the Cytofluorograph (1971) from Bio/Physics Systems Inc. (later: Ortho Diagnostics), the PAS 8000 (1973) from Partec, the first FACS (fluorescence-activated cell sorting) instrument from Becton Dickinson (1974), the ICP 22 (1975) from Partec/Phywe and the Epics from Coulter (1977/78). (
  • The first fluorescence-based flow cytometry device (ICP 11) was developed in 1968 by Wolfgang Göhde from the University of Münster, filed for patent on 18 December 1968 and first commercialized in 1968/69 by German developer and manufacturer Partec through Phywe AG in Göttingen. (
  • citation needed] The original name of the fluorescence-based flow cytometry technology was "pulse cytophotometry" (German: Impulszytophotometrie), based on the first patent application on fluorescence-based flow cytometry. (
  • Progress through case studies related to lymphocyte evaluation using flow cytometry while performing final interpretations. (
  • In the present study we show that B-cell transformation by EBV, lymphocyte viability, and T-cell proliferation in response to solid phase anti-CD3/anti-CD28 plus IL-2 are maintained over time in cryopreserrved whole blood. (
  • The Flow Cytometry Core is a state-of-the-art facility to accommodate a range of cell sorting and analysis needs. (
  • A wide variety of applications are supported including high speed cell sorting, 15-color cell analysis, cytokine analysis by multiplex bead arrays, imaging flow cytometry, phoshoprotein analysis, and cell cycle analysis. (
  • Calculate absolute cell counts from percentage values for flow cytometry analysis. (
  • Identify blood cell types associated with respective specific surface markers. (
  • What cell population is gated in this peripheral blood sample case analyzed by flow cytometry? (
  • The combination of these two dyes makes it easy to clearly distinguish normal, apoptotic, and dead-cell populations by flow cytometry. (
  • Flow cytometry is known for cell counting, cell sorting and cancer biomarker discovery. (
  • For obvious reasons, the knowledge we have of such aspects is derived from examination of placental tissues at delivery and/or of peripheral blood, with the latter providing the only accessible window through which one can view changes in cell numbers and phenotypes as a function of gestational age. (
  • Red Blood Cell Lysis Solution (10×) is a ammonium chloride (NH4Cl) based solution, pH 7.5. (
  • We and others have reported that EPCs isolated from cord blood have a greater proliferative potential and a higher cell cycle rate than EPCs from other sources, suggesting that cord blood-derived EPCs may more effectively contribute to therapeutic vasculogenesis ( 11 , 12 , 16 ). (
  • Krutzik, P. O., & Nolan, G. P. (2003), Intracellular phospho‐protein staining techniques for flow cytometry: Monitoring single cell signaling events. (
  • The CD4 cell counts were obtained by using the Becton Dickinson MultiTEST reagent in TrueCOUNT tubes (Becton Dickinson Immunocytometry Systems, San Jose, CA). Comparison of this method with CD4 counts calculated from CBC absolute lymph counts from fresh whole blood X CD4% from the frozen whole blood resulted in a correlation coefficient of 0.9872. (
  • Characterization of extracellular vesicles in whole blood: Influence of pre-analytical parameters and visualization of vesicle-cell interactions using imaging flow cytometry. (
  • Complete blood count (CBC) with blood cell differential . (
  • People with CLL usually have a high white blood cell count . (
  • The specimen should also include a peripheral blood smear and the results of the most recent complete blood count and white blood cell differential. (
  • They also upregulate CCR7, a chemotactic receptor that induces the dendritic cell to travel through the blood stream to the spleen or through the lymphatic system to a lymph node. (
  • When local ischemia occurs, EPCs are mobilized from bone marrow to the peripheral blood. (
  • EPCs can be isolated from the bone marrow, cord blood, and peripheral blood. (
  • Cryopreserved whole blood was thawed at room temperature, washed with RPMI 1640 plus 5 m m EDTA (Life Technologies, Inc.), and diluted in RPMI 1640 supplemented with 10% FCS. (
  • After informed consent, patients provided a peripheral blood sample, and plasma and PBMCs were isolated from whole blood in CPT tubes (Becton-Dickinson). (
  • Primary outcomes included C-peptide area under the curve (AUC) following a mixed-meal tolerance test (MMTT) and flow cytometry. (
  • Therefore, there were 7 cases which were negative for RBC bound IgG by Gel card test and these were positive by flow-cytometry. (
  • New York Clients: The BTK / Btk Protein Flow portion of this test is not New York State approved. (
  • The flow cytometry-based test thus became the worldwide gold standard for AIDS diagnosis, and one of the key tools widely adopted in the global efforts to treat sufferers of the disease 1 . (
  • CD-Chex Plus® is a whole blood positive procedure control with assayed values used to monitor reagent staining, erythrocyte lysis, sample preparation, and instrument performance. (
  • 15 ) showed that vascular endothelial growth factor (VEGF) gene transfer significantly increased nerve conduction velocity and nerve blood flow as well as the amount of vasculature in the muscles and nerves, suggesting that the induction of local angiogenesis ameliorates experimental neuropathy. (
  • This document provides guidance for the performance of reticulocyte counting by flow cytometry. (
  • In this report, the global Blood Conservation System market is valued at USD XX million in 2017 and is expected to reach USD XX million by the end of 2025, growing at a CAGR of XX% between 2017 and 2025. (
  • Used to optimize fluorescence compensation settings for multicolor flow cytometric analyses. (
  • No other laboratory method provides as rapid and detailed analysis of cellular populations as flow cytometry, making it a valuable tool for diagnosis and management of several hematologic and immunologic diseases. (
  • Flow cytometry is an important tool for the diagnosis and classification of leukemia. (
  • The large data sets obtained by this technique would improve diagnosis accuracy, reduce imaging time, and open new possibilities for noninvasive monitoring of blood in patients. (
  • Flow cytometry and mass spectrometry are widely used analytical tools in cancer research. (
  • One component of sample collection in the Prostate, Lung, Colorectal and Ovarian Cancer Screening Trial involves shipment of blood in acid-citrate-dextrose anticoagulant to a central processing laboratory, where 10% DMSO is added, and whole blood aliquots are cryopreserved. (
  • One 300 mcg dose of RhIG protects against a FMH of 30 mL of D-positive fetal whole blood or 15 mL of D-positive fetal RBCs. (