The fraction of a blood sample, following CENTRIFUGATION, that is distinguished as a thin light-colored layer between the RED BLOOD CELLS, underneath it, and the PLASMA, above it. It is composed mostly of WHITE BLOOD CELLS and PLATELETS.
Linear furanocoumarins which are found in many PLANTS, especially UMBELLIFERAE and RUTACEAE, as well as PSORALEA from which they were originally discovered. They can intercalate DNA and, in an UV-initiated reaction of the furan portion, alkylate PYRIMIDINES, resulting in PHOTOSENSITIVITY DISORDERS.
The transfer of blood platelets from a donor to a recipient or reinfusion to the donor.
Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation.
The preparation of platelet concentrates with the return of red cells and platelet-poor plasma to the donor.
The attachment of PLATELETS to one another. This clumping together can be induced by a number of agents (e.g., THROMBIN; COLLAGEN) and is part of the mechanism leading to the formation of a THROMBUS.
A child whose needs, abilities, or other characteristics vary so much from the average in mental, physical, or social areas that a greater than usual level of services is needed to facilitate the child's maximum potential development.
An order of heteroxenous protozoa in which the macrogamete and microgamont develop independently. A conoid is usually absent.
Infections with unicellular organisms formerly members of the subkingdom Protozoa. The infections may be experimental or veterinary.
Diseases of birds not considered poultry, therefore usually found in zoos, parks, and the wild. The concept is differentiated from POULTRY DISEASES which is for birds raised as a source of meat or eggs for human consumption, and usually found in barnyards, hatcheries, etc.
A phylum of unicellular parasitic EUKARYOTES characterized by the presence of complex apical organelles generally consisting of a conoid that aids in penetrating host cells, rhoptries that possibly secrete a proteolytic enzyme, and subpellicular microtubules that may be related to motility.
Warm-blooded VERTEBRATES possessing FEATHERS and belonging to the class Aves.
A superfamily of nematodes of the suborder SPIRURINA. Its organisms possess a filiform body and a mouth surrounded by papillae.
Small uniformly-sized spherical particles, of micrometer dimensions, frequently labeled with radioisotopes or various reagents acting as tags or markers.
Central retinal artery and its branches. It arises from the ophthalmic artery, pierces the optic nerve and runs through its center, enters the eye through the porus opticus and branches to supply the retina.
The destruction of ERYTHROCYTES by many different causal agents such as antibodies, bacteria, chemicals, temperature, and changes in tonicity.
A chemical system that functions to control the levels of specific ions in solution. When the level of hydrogen ion in solution is controlled the system is called a pH buffer.
Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.
Inorganic salts of sulfurous acid.
Addition of methyl groups to DNA. DNA methyltransferases (DNA methylases) perform this reaction using S-ADENOSYLMETHIONINE as the methyl group donor.
Measurement of the intensity and quality of fluorescence.
Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.
A heat stable DNA-DIRECTED DNA POLYMERASE from the bacteria Thermus aquaticus. It is widely used for the amplification of genes through the process of POLYMERASE CHAIN REACTION. EC 2.7.7.-.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
Exclusive legal rights or privileges applied to inventions, plants, etc.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
Bleeding within the brain as a result of penetrating and nonpenetrating CRANIOCEREBRAL TRAUMA. Traumatically induced hemorrhages may occur in any area of the brain, including the CEREBRUM; BRAIN STEM (see BRAIN STEM HEMORRHAGE, TRAUMATIC); and CEREBELLUM.
Agents that prevent clotting.
The process of the interaction of BLOOD COAGULATION FACTORS that results in an insoluble FIBRIN clot.
A process of separating particulate matter from a fluid, such as air or a liquid, by passing the fluid carrier through a medium that will not pass the particulates. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A novel composition, device, or process, independently conceived de novo or derived from a pre-existing model.

Transcription of Alu DNA elements in blood cells of sporadic Creutzfeldt-Jakob disease (sCJD). (1/25)

Alu DNA elements were long considered to be of no biological significance and thus have been only poorly defined. However, in the past Alu DNA elements with well-defined nucleotide sequences have been suspected to contribute to disease, but the role of Alu DNA element transcripts has rarely been investigated. For the first time, we determined in a real-time approach Alu DNA element transcription in buffy coat cells isolated from the blood of humans suffering from sporadic Creutzfeldt-Jakob disease (sCJD) and other neurodegenerative disorders. The reverse transcribed Alu transcripts were amplified and their cDNA sequences were aligned to genomic regions best fitted to database genomic Alu DNA element sequences deposited in the UCSC and NCBI data bases. Our cloned Alu RNA/cDNA sequences were widely distributed in the human genome and preferably belonged to the "young" Alu Y family. We also observed that some RNA/cDNA clones could be aligned to several chromosomes because of the same degree of identity and score to resident genomic Alu DNA elements. These elements, called paralogues, have purportedly been recently generated by retrotransposition. Along with cases of sCJD we also included cases of dementia and Alzheimer disease (AD). Each group revealed a divergent pattern of transcribed Alu elements. Chromosome 2 was the most preferred site in sCJD cases, besides chromosome 17; in AD cases chromosome 11 was overrepresented whereas chromosomes 2, 3 and 17 were preferred active Alu loci in controls. Chromosomes 2, 12 and 17 gave rise to Alu transcripts in dementia cases. The detection of putative Alu paralogues widely differed depending on the disease. A detailed data search revealed that some cloned Alu transcripts originated from RNA polymerase III transcription since the genomic sites of their Alu elements were found between genes. Other Alu DNA elements could be located close to or within coding regions of genes. In general, our observations suggest that identification and genomic localization of active Alu DNA elements could be further developed as a surrogate marker for differential gene expression in disease. A sufficient number of cases are necessary for statistical significance before Alu DNA elements can be considered useful to differentiate neurodegenerative diseases from controls.  (+info)

Methylprednisolone prevents tumour necrosis factor-alpha-dependent multinucleated giant cell formation. (2/25)

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The Mirasol Pathogen Reduction Technology system and quality of platelets stored in platelet additive solution. (3/25)

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Effects of storage time and leucocyte burden of packed and buffy-coat depleted red blood cell units on red cell storage lesion. (4/25)

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Development and validation of a new, sensitive immunochemical assay for O(6)-methylguanine in DNA and its application in a population study. (5/25)

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IL-4 and IL-13 alter plasmacytoid dendritic cell responsiveness to CpG DNA and herpes simplex virus-1. (6/25)

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Merkel cell polyomavirus DNA sequences in the buffy coats of healthy blood donors. (7/25)

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The histamine H4 receptor is highly expressed on plasmacytoid dendritic cells in psoriasis and histamine regulates their cytokine production and migration. (8/25)

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Pathogen reduction technology to be used in combating bloodborne infectious diseases Lakewood, Colo., USA-19 November 2014- Due to public health concerns over the transmission of chikungunya virus (CHIKV) and dengue fever, Terumo BCT will file a submission to the U.S. Food and Drug Administration (FDA) for expanded access use of the Mirasol® Pathogen Reduction Technology System for the treatment of platelets. The expanded access provision is meant to facilitate the availability of promising new therapies and diagnostic devices to patients as early in the development cycle as possible. The Mirasol system is generally used on platelets to reduce pathogens for which no comparable or satisfactory alternative exists. The submission will include Banco de Sangre de Servicios Mutuos in Puerto Rico as an investigational site where the Mirasol system can treat platelets stored in plasma or platelet additive solution (PAS). The Mirasol system is a pathogen-reduction technology designed to render a broad ...
CaridianBCT, a developer and marketer of blood collection and processing systems to hospitals and blood banks, has initiated a clinical trial for treating whole blood with Mirasol pathogen reduction technology after receiving clearance from the FDA and US Department of Defense. The Mirasol pathogen reduction technology (PRT) system is a proprietary technology that uses riboflavin and UV light to reduce the levels of infectious pathogens and inactivate white cells from collected blood, improving the safety of transfused blood. The study, which has started enrolling subjects, will take six to 12 months to complete and is a feasibility study designed to support filing for regulatory approvals in the US. The trial is sponsored by CaridianBCT Biotechnologies and is being conducted at the Hoxworth Blood Center in Cincinnati, Ohio. CaridianBCT Biotechnologies has received research funding from the US Department of Defense with the goal to develop a transportable system to treat whole blood and reduce ...
LENEXA, Kan., August 25, 2016 Mediware Information Systems, Inc. is pleased to announce the release of LifeTrak 2015 R2, the first module in the blood establishment computer software (BECS) market to provide the ability for blood centers to capture data related to the use of pathogen reduction (PR) technology.. The PR addition to the LifeTrak Lab - Component Manufacturing module was independently developed in consultation with Cerus Corporation, whose INTERCEPT® Blood System for platelets and plasma is designed to inactivate a wide range of viral, bacterial, and parasitic pathogens that may be present in donated blood. The LifeTrak Lab PR processes are compatible with the INTERCEPT system and can monitor, upload, and check relevant INTERCEPT PR data to aid in data capture and product conformance as a result of the manufacturing controls available in LifeTrak Lab.. The new LifeTrak module provides our common customers with a means of adding INTERCEPT PR process data to their existing LifeTrak ...
Bacterial contamination and emerging infections combined with increased international travel pose a great risk to the safety of the blood supply. Tests to detect the presence of infection in a donor h
Bone marrow stimulation with G-CSF (Neupogen®, Granocyte®) is only recommended for febrile neutropenia which does not respond to antibiotic treatment, severe neutropenia (granulocytes , 0.5 x 109 /L for more than 1 week), and in cases where it is necessary to administer curative treatment with sufficient dosage intensity.. ...
Bone marrow stimulation with G-CSF (Neupogen®, Granocyte®) is only recommended for febrile neutropenia which does not respond to antibiotic treatment, severe neutropenia (granulocytes , 0.5 x 109 /L for more than 1 week), and in cases where it is necessary to administer curative treatment with sufficient dosage intensity.. ...
ElectronicHealth Records: Adoption of New Technology Systems. Studentsname. ElectronicHealth Records: Adoption of New Technology Systems. Advancementin technology in the health sector has helped improve the waypatients are being handled and also in the management of diseases(Cresswell & Sheikh, 2009). Embracing the E.H.R will have apositive effect on the working efficiency in hospitals. Below arefive steps to be followed while trying to convince the nurses toadopt the new technology.. Thefirst step of approaching the nurses will be providing them withgeneral knowledge on the new technology that is to be adopted. Thiswill include giving a thorough explanation of how the system works tothe point that every nurse gets well acquainted (Murphy, 2011). Thiswill provide them with a sharp insight that the system is verycompatible with the existing values and practices in nursing. Theelectronic health record contains patient management section,clinical section, laboratory section and billing system ...
Currently some pathogen-reduced platelet products (PR-PCs) have passed phase III studies, are in progress or can be expected in the near future. At present some transfusion centers throughout Europe have implemented PR-PCs, but as yet PR-PCs are not formally accepted as a standard product that should be applied nation-wide. Because many uncertainties currently exist on the optimal platelet product, it is in the interest of patients, health care providers and the transfusion provider (Sanquin) to decide on evidence.. With all the current safety measures remaining in place, pathogen reduction provides a safety benefit by reducing the number of transfusions of platelet concentrates contaminated with bacteria, but which were missed by the screening method. In the Dutch situation, morbidity is estimated to be 1:14,000 platelet concentrates [Te Boekhorst, Transfusion 2005]. In this publication, two cases of transmission of B. cereus by a platelet transfusion are reported, where both patients ...
The U.S. Food and Drug Administration yesterday approved the Intercept Blood System for platelets, the first pathogen reduction system to treat single donor apheresis platelets. The system is for use by blood establishments that collect and manufacture blood and blood components to prepare pathogen reduced platelets for transfusion to reduce the risk of transfusion-transmitted infections ...
Background We recently developed and characterized a standardized and clinical grade human Platelet Lysate (hPL) that constitutes an advantageous substitute for fetal bovine serum (FBS) for human mesenchymal stem cell (hMSC) expansion required in cell therapy procedures, avoiding xenogenic risks (virological and immunological) and ethical issues. Because of the progressive use of pathogen-reduced (PR) labile blood components, and the requirement of ensuring the viral safety of raw materials for cell therapy products, we evaluated the impact of the novel procedure known as THERAFLEX UV-Platelets for pathogen reduction on hPL quality (growth factors content) and efficacy (as a medium supplement for hMSC expansion). This technology is based on short-wave ultraviolet light (UV-C) that induces non-reversible damages in DNA and RNA of pathogens while preserving protein structures and functions, and has the main advantage of not needing the addition of any photosensitizing additives (that might secondarily
Why do we perform research activities in a blood bank? Our primary mission is to deliver efficient and safe blood products to our patients. This simple mission has not changed for decades but its interpretation is highly dependent upon the medical and scientific context and the technologies available. Transfusion medicine has been completely reshaped, from the use of glass bottles to store blood products, and limited screening technologies till the seventies, followed by the introduction in the eighties of plastic bags that guarantee complete sterility from the donor to the patient, the generalisation of serologic screening and the introduction of nucleic acid testing , that allowed to reduce the risk of pathogen transmission through the transfusion chain to unprecedently low levels, and by the use of additive solutions to improve the storage of red blood cells and platelets. Recently, we have introduced universal pathogen reduction technologies for platelets, and will soon introduce it for ...
The PRESS trial is a single-center, two-part, randomized cross-over pilot study in Copenhagen that will evaluate 1) the safety and performance of Mirasol-treated and untreated platelet products stored for 2-4 days and for 7-days in Platelet Additive Solution (PAS) and 2) the correlation between the TEG® parameters and platelet count increments after platelet transfusions in thrombocytopenic subjects ...
March 25, 2019, a Request for Information (RFI) for Funding Opportunity Announcement DE-FOA-0002109, entitled Strategies for Improving or Creating Emission Reduction Technology and Byproduct Utilization for Hazardous Substances Resulting from Coal Combustion. Contract Specialist Ashley Reichl. Response due date is May 6, 2019. Instructions can be found for this RFI at FedConnect. ...
Dr. Snyders research relates to the broad field of Transfusion Medicine and, more specifically, Transfusion Oncology and Transfusion Immunobiology. His current research includes an on-going evaluation of the platelet storage lesion. This includes evaluation of alterations that occur in platelet metabolism during various storage conditions, in the platelets cystoskeleton and in the mitochondria within platelets. Studies of the in vitro and in vivo aspects of platelet storage are an on-going effort in the laboratory. Dr. Snyder also has a long-term history of investigating the role of leukodepletion in blood components. Most recently, the laboratory has turned to the evaluation of new pathogen reduction technology. This includes the addition of various compounds to blood to remove known and potentially dangerous, as well as unknown, viral, bacterial, and protozoal contaminants in donated blood. The laboratory has a special expertise in radiolabeling human blood cells. The laboratory is currently ...
Platelets are either isolated from collected units of whole blood and pooled to make a therapeutic dose, or collected by platelet apheresis: blood is taken from the donor, passed through a device which removes the platelets, and the remainder is returned to the donor in a closed loop. The industry standard is for platelets to be tested for bacteria before transfusion to avoid septic reactions, which can be fatal. Recently the AABB Industry Standards for Blood Banks and Transfusion Services (5.1.5.1) has allowed for use of pathogen reduction technology as an alternative to bacterial screenings in platelets.[61]. Pooled whole-blood platelets, sometimes called random platelets, are separated by one of two methods.[62] In the US, a unit of whole blood is placed into a large centrifuge in what is referred to as a soft spin. At these settings, the platelets remain suspended in the plasma. The platelet-rich plasma (PRP) is removed from the red cells, then centrifuged at a faster setting to harvest ...
Platelets are either isolated from collected units of whole blood and pooled to make a therapeutic dose, or collected by platelet apheresis: blood is taken from the donor, passed through a device which removes the platelets, and the remainder is returned to the donor in a closed loop. The industry standard is for platelets to be tested for bacteria before transfusion to avoid septic reactions, which can be fatal. Recently the AABB Industry Standards for Blood Banks and Transfusion Services (5.1.5.1) has allowed for use of pathogen reduction technology as an alternative to bacterial screenings in platelets.[56] Pooled whole-blood platelets, sometimes called random platelets, are separated by one of two methods.[57] In the US, a unit of whole blood is placed into a large centrifuge in what is referred to as a soft spin. At these settings, the platelets remain suspended in the plasma. The platelet-rich plasma (PRP) is removed from the red cells, then centrifuged at a faster setting to harvest ...
Blood centers are faced with many challenges including maximizing production yield from the blood product donations they receive as well as ensuring the highest possible level of safety for transfusion patients, including protection from transfusion transmitted diseases. This must be accomplished in a fiscally responsible manner which minimizes operating expenses including consumables, equipment, waste, and personnel costs, among others.. Several methods are available to produce platelet concentrates for transfusion. One of the most common is the buffy coat method in which a single therapeutic platelet unit (,= 2.0 x10(11) platelets per unit or per local regulations) is prepared by pooling the buffy coat layer from up to six whole blood donations. A procedure for producing double dose whole blood derived platelets has only recently been developed.. Presented here is a novel method for preparing double dose whole blood derived platelet concentrates from pools of 7 buffy coats and subsequently ...
The StraightFrom® Buffy Coat CD4 MicroBead Kit has been developed for the rapid positive selection of CD4+ cells directly from one buffy coat using the MultiMACS™ Cell24 Separator Plus for semi-automated separation or a MACS® Separator for manual separation. No sample preparation is required, including density gradient centrifugation, erythrocyte lysis, wash steps, or cell count. Whole Blood Columns and Whole Blood Column Elution Buffer are included in the kit to allow direct processing of an entire buffy coat. - Suomi
The StraightFrom® Buffy Coat CD8 MicroBead Kit has been developed for the rapid positive selection of CD8+ cells directly from one buffy coat using the MultiMACS™ Cell24 Separator Plus for semi-automated separation or a MACS® Separator for manual separation. No sample preparation is required, including density gradient centrifugation, erythrocyte lysis, wash steps, or cell count. Whole Blood Columns and Whole Blood Column Elution Buffer are included in the kit to allow direct processing of an entire buffy coat. - Norge
0043] FIG. 6 shows a flowchart of a process 20 for a computer-implemented method for manipulating data presented in various forms, in a graphical user interface of an information technology system. The process 20 comprises the following process blocks: [0044] Block 21: Perform statistical analysis of a data set using a statistical process to generate a graphical statistical representation of the data set. [0045] Block 22: Display the data set and its graphical statistical representation in a user interface (UI) on a display screen. [0046] Block 23: Display a GUI widget in the UI for allowing interactive user adjustment (manipulation) of a statistical range for the graphical statistical representation. [0047] Block 24: Receive user adjustment of the statistical range. [0048] Block 25: Filter the data set based on the adjusted statistical range, wherein the filtered data set corresponds to the graphical statistical representation within the adjusted statistical range. For example, if user adjusted ...
Genetic Programming for Automating the Development of Data Management Algorithms in Information Technology Systems. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Drilling is ongoing at Mirasol, with the principal focus being the Central Zone where the mineralization remains open to expansion at depth and along strike in both directions. Additional drill pads have also been constructed for the Mirasol Northwest drilling area, where hole Z12-76 encountered silver grades at depth (335.2 g/t over 1.58 meters) and the mineralization is open to expansion at depth and along strike in both directions. Brian Hall, M.Sc., P. Geo., Nigel Hulme, P. Geo., and Wojtek Jakubowski, P. Geo., Qualified Persons under the meaning of Canadian National Instrument 43-101, are responsible for the technical content of this news release. IMPACT Silver Corp. is a precious metal mining and exploration company operating in central Mexico, with a producing silver operation at the 423-square-kilometer Royal Mines of Zacualpan Silver-Gold District which includes the Mirasol, and the adjoining 200-square-kilometer Mamatla VMS District which includes the advanced Capire Mine Development ...
Pour plus de détails, cliquez sur limage. Tourcoing - October 29, 2014 - Macopharma is pleased to inform you that a current article from the Blood Centre of Galicia, Santiago de Compostela, Spain, entitled In Vitro evaluation of platelet concentrates suspended in additive solution, and treated for pathogen REDUCTION: Effects of clumping formation. has been recently published in the journal Blood Transfusion.. The aim of this study was to analyse the relationship between the occurrence of clumping and their effect on in vitro quality of platelet concentrates suspended in additive solution.. No significant difference was found throughout storage between the groups. The LDH levels increased in both groups, but this increase was higher in the test group at the last day of testing, without significant difference on day 2. In contrast, pH values on day 2 were significantly different between test and control group. Platelet derived cytokines increased comparably during storage.. The authors ...
The Maxwell RSC Buffy Coat DNA Kit is a simple, automated method for extraction of genomic DNA from 50-250µl of buffy coat samples using the Maxwell RSC Instrument.
Human fibroblasts, and specifically embryonic or neonatal fibroblasts, have represented the conventional cell substrate for recovery of HCMV from clinical samples since the beginning of medical virology. Due to the increasing need for recovery of HCMV from the blood of immunocompromised patients, we investigated whether use of HCMV-free HUVEC could increase the recovery rate of HCMV from blood (buffy coat) samples in comparison to HELF. Clinical samples other than blood were inoculated in parallel onto the two cell substrates as controls. Surprisingly, while the isolation rate from blood samples did not increase on HUVEC compared to HELF, no or very little virus could be recovered on primary isolation on HUVEC from clinical samples other than blood, even when virus was present in exceedingly large amounts. Thus, HELF were confirmed as the cell substrate of choice for isolation of HCMV from the broadest series of clinical samples, whereas HCMV recovery on HUVEC was substantially restricted to ...
A toolkit named for sequence reads quality control, which consists of six programs for reads quality assessment, reads filtration and generation of graphic reports. The HTQC toolkit can generate reads quality assessment faster than existing tools, providing guidance for reads filtration utilities that allow users to choose different strategies to remove low quality reads.
Stage A: To evaluate the safety and efficacy of the INTERCEPT Blood System for Red Blood Cells Pathogen Reduction Treatment (PRT) in comparison to conventional RBCs in adult patients who require RBC transfusion support. Stage B: To provide early access to the INTERCEPT pathogen reduction system for RBC in regions where a substantial proportion of the population has been infected or is at risk of infection by the Zika virus (ZIKV), and the risk of asymptomatic infection among qualified blood donors is recognized. Besides the reduction of risk of transfusion transmitted ZIKV, the intent of the study is also to reduce the risk of transfusion-transmitted infections (TTI) in general, including transfusion related sepsis and other emerging or concurrent endemic pathogens (e.g. Dengue and Chikungunya), and to reduce the risk of TA-GVHD. As part of this treatment use study, additional data will be provided on the safety of INTERCEPT-treated RBCs (IBS RBCs) supplied for routine clinical transfusion ...
The INTERCEPT Blood System - pathogen inactivation for platelet and plasma components. INTERCEPT is designed to reduce the risk of transfusion-transmitted diseases by inactivating a broad range of pathogens such as viruses, bacteria and parasites that may be present in donated blood.
The INTERCEPT Blood System - pathogen inactivation for platelet and plasma components. INTERCEPT is designed to reduce the risk of transfusion-transmitted diseases by inactivating a broad range of pathogens such as viruses, bacteria and parasites that may be present in donated blood.
On August 19, Transfusion published a scientific paper by PPTA member companies on the effectiveness of the nanofiltration method in assuring the safety of plasma derivatives during manufacturing. The publication shows that this manufacturing step effectively retains a wide range of viruses from different plasma derivatives, including those which may be transmitted through transfusion, such as HIV, as well as those which are resistant to other steps with pathogen reduction or removal capacity, such as small, non-enveloped viruses (including Parvovirus B19). The results represent one of the most extensive data collections on this topic to date regarding the range of plasma derivatives, viruses, and different manufacturing conditions studied. This is fourth publication on the efficacy of pathogen reduction/ removal methodologies by PPTA member companies; other publications have shown the effectiveness of prion removal, cold ethanol fractionation and solvent-detergent (S/D) treatment in assuring ...
This four-hour workshop helps participants understand the production of hydrogen with electricity from solar, wind, and water power, newest technologies used in a hydrogen production, and fuel cell technology and applications in hydrogen vehicles.. ...
1. Taniguchi Ishikawa E*, Chang KH*, Nayak R, Olsson HA, Ficker AM, Dunn SK, Madhu MN, Sengupta A, Whitsett JA, Grimes HL, Cancelas JA. Klf5 controls bone marrow homing of stem cells and progenitors through Rab5-mediated membrane β1/β2-integrin expression. Nat Commun 2013 Apr 3;4:art. No. 1660. doi: 10.1038/ncomms2645. PMID: 23552075, PMCID: 3627399.. 2. Dumont LJ*, Cancelas JA*, Graminske S, Friedman KD, Vassallo RR, Whitley PH, Rugg N, Dumont DF, Herschel L, Siegal AH, Szczepiorkowski ZM, Fender L, Razatos A. In vitro and in vivo quality of leukocyte-reduced apheresis platelets stored in a new platelet additive solution. Transfusion 2013, May;53(5):972-80. PMID: 22882530. 3. Chang KH, Sanchez-Aguilera A, Shen S, Sengupta A, Madhu MN, Ficker AM, Dunn SK, Kuenzi AM, Anrett JL, Santho RA, Agirre X, Perentesis JP, Deininger MW, Zheng Y, Bustelo XR, Williams DA, Cancelas JA. Vav3 collaborates with p190-BCR-ABL in lymphoid progenitor leukemogenesis, proliferation and survival. Blood 2012 Jul ...
White Cell Concentrate (Buffy Coat). In: MA L, MS S, RE F, N W. MA L, MS S, RE F, N W Eds. Lichtman MA, et al.eds. Lichtmans Atlas of Hematology 2016 New York, NY: McGraw-Hill; . http://hemonc.mhmedical.com/content.aspx?bookid=1630§ionid=116917323. Accessed January 22, 2018 ...
Single Donor Human Buffy Coats Leukocytes from Innovative Research was used in the following study: Coupling Fluorescence-Activated Cell Sorting and Targeted Analysis of Histone Modification Profiles in Primary Human Leukocytes Jeannie M. Camarillo, Suchitra Swaminathan, Nebiyu A. Abshiru, Jacek W. Sikora, Paul M. Thomas, Neil L. Kelleher Journal of The American Society for Mass Spectrometry 08 July 2019 …Histone posttranslational modifications (PTMs) are essential for regulating chromatin and maintaining gene expression throughout cell differentiation. Despite the deep level of understanding of immunophenotypic differentiation pathways in hematopoietic cells, few studies have investigated global levels of histone PTMs required for differentiation.... ...
Dow offers the broadest range of fuel marker technology and dye solutions in the marketplace, setting the industry standard with innovative solutions.
BACKGROUND A designer typically has to make several compromises in the selection of materials. The preferred material may not be useable due to a lack of essential surface properties for the application. In the case of products made of plastics, the balancing of bulk to surface property requirements has always been the Achilles heel of optimum design. For example, the use of polypropylene, a low cost resin, may be the preferred material for a specific packaging application. Preferred because of its processability, weight, strength and cost, but unusable by itself because of its gas permeation properties.. The methods available to the processor for modifying surface properties have been limited to flame or corona treatment or wet chemical techniques. Flame and corona treatments, though useful in oxidizing the surfaces of plastics, have limited utility in many applications and the transitory nature of these modifications prevent their widespread use in many situations. Unlike plasma discharges ...
The Library Information Technology Association sponsors four LITA scholarships to encourage the entry of qualified persons into the library and information technology field ...
A sudden shortage of physician resources due to overwhelming patient needs can affect the quality of care in the emergency department (ED). Developing effective response strategies remains a challenging research area. We created a novel system using information and communication technology (ICT) to respond to a sudden shortage, and tested the system to determine whether it would compensate for a shortage. Patients (n = 4890) transferred to a level I trauma center in Japan during 2012-2015 were studied. We assessed whether the system secured the necessary physicians without using other means such as phone or pager, and calculated fulfillment rate by the system as a primary outcome variable. We tested for the difference in probability of multiple casualties among total casualties transferred to the ED as an indicator of ability to respond to excessive patient needs, in a secondary analysis before and after system introduction. The system was activated 24 times (stand-by request [n = 12], attendance
Information technology touches all aspects of teaching, learning and research. ITSS supports the mission of Saint Marys University by connecting faculty, staff and students to the information technology resources they need.. We are here to help - whether you want to connect to the SMU wireless network, activate your computer accounts, set up email, or access any of our other services. Get in touch. Contact the Help Desk by email [email protected] or phone: 902-496-8111. Access our Services. IT Quick Tip: Internet Explorer vulnerability. Microsoft has released a patch for security vulnerability found in all versions of Internet Explorer. To install the patch, Click on Start , All Programs , Windows Update.. ...
Contemporary healthcare facilities are filled with technology systems increasingly sophisticated and complex. Properly planned and managed, these systems offer enormous benefit to patients, clinicians and support staff. Unfortunately, these systems are often disparate and unable to share data between systems, resulting in inefficiencies, lost opportunities and cost over-runs. As patient satisfaction, clinician need, compliance issues and regulatory demands are driving the unique application of technology to Healthcare, institutions increasingly prefer a comprehensive approach, unifying infrastructure, middleware and disparate systems into a common topology. Strategically-planned Healthcare Technology Systems improve patient outcomes and reduce costs by integrating clinical devices to the Electronic Health Record (EHR), managing assets, reducing errors, managing alerts, improving communications, tracking patient / clinician location, improving clinician efficiency, speeding bed-ready systems, and ...
3 Project Overview Document the findings, analysis, conclusions and recommendations of the Team-2 based on their work on six LSSQTT tools  Tool #7: Team building, and communicating the project and culture  Tool #8: Technological system leadership for change and improvement  Tool #9: Assessing technological infrastructure for innovation  Tool #10: Foundational Infrastructure to standardize improvement  Tool #11:Robust Design for New Product Development, Innovation  Tool #12: Assessing technological decision making  Follow an excel based documentation framework that confirms to ISO 9000 Context: A simulated project involving construction of a server room/data center as it relates to disaster recovery
Noble feed gases such as Argon are used since they tend to initiate cleavage without grafting to the surface. Treatment times are typically short so as to reduce further unwanted chemistry.. Excessive treatment results in attack of the base polymer which ultimately ashes the entire polymeric part resulting in its destruction. A typical cleaning procedure for polyethylene would be to treat with Argon at a pressure of 0.01 to 0.4 Torr, with a power density of 0.5 W/cm2 at 13.56 MHz rf on parallel-plate electrodes. Once contaminants are removed, a more stable polymer surface is exposed to the plasma environment so as to facilitate further reaction such as etching, grafting or direct application of the desired surface coating. More aggressive processing can be achieved by using reactive gases or by use of electrical biasing to increase the energetics of the bombarding ions. However, etching into the base polymer begins to occur, which leads us logically to the next topic.. ETCHING. To obtain ...
A technical report outlining pediatric-specific needs for software used in the inpatient setting, but it also largely applicable for other clinical settings. From the American Academy of Pediatrics Council on Clinical Information Technology.. Lehmann 2015 (Pediatrics) , PubMed 25713282 , Author Search ...
A computer-implemented method and system for assessing the overall risk in at least part of an information technology system includes inputting into a risk assessment database a plurality of identified risks in a system; associating the risks to at least one severity band in a risk echelon; assigning a value to each risk; multiplying each risk value by a coefficient factor; and summing the factored risk values to determine the overall risk. The method preferably includes modifying the security implementation of the information technology system and determining the modified overall risk. The system preferably includes an automated vulnerability detection scanner to gather risk information, which is stored on a database and used in calculating the overall risk.
UAB is an Equal Opportunity/Affirmative Action Employer committed to fostering a diverse, equitable and family-friendly environment in which all faculty and staff can excel and achieve work/life balance irrespective of race, national origin, age, genetic or family medical history, gender, faith, gender identity and expression as well as sexual orientation. UAB also encourages applications from individuals with disabilities and veterans ...
UAB is an Equal Opportunity/Affirmative Action Employer committed to fostering a diverse, equitable and family-friendly environment in which all faculty and staff can excel and achieve work/life balance irrespective of race, national origin, age, genetic or family medical history, gender, faith, gender identity and expression as well as sexual orientation. UAB also encourages applications from individuals with disabilities and veterans ...
Journal of Medical Internet Research - International Scientific Journal for Medical Research, Information and Communication on the Internet
Most RBC components available today are derived from the collection of 450 to 500 mL of whole blood into sterile plastic bags containing citrate-phosphate-dextrose (CPD) anticoagulant. The whole blood is spun to sediment out the RBCs, and most of the plasma is removed by pushing it into a preattached satellite bag. Generally, 100 to 110 mL of a nutrient additive solution is added back to the packed RBCs, creating an additive RBC product that has a final hematocrit of 55% to 60% (0.55 to 0.60). A variety of additive solutions are in use today, each of which contains a particular mix of glucose, adenine, and in some cases, mannitol (Table⇓). These solutions prolong the shelf life of the RBC product from 21 days (packed RBCs in CPD) to 42 days (additive RBCs). A transfusion of 10 mL/kg of additive RBCs would be expected to raise the patients hematocrit by 7% to 8% (0.07 to 0.08).. ...
At the recent VIV Asia in Bangkok, Nutriad presented solutions for reduction of antibiotics usage in livestock production. This years VIV saw a record attendance and served as an excellent platform for sharing insights on how feed additives can help the reduction of non-therapeutic use of antibiotics. Nutriad attended industry experts from across the world at its booth and participated in several conferences and seminars during the week.
TY - JOUR. T1 - Red blood cell storage lesion. AU - Kor, Daryl J. AU - Van Buskirk, Camille M.. AU - Gajic, Ognjen. PY - 2009. Y1 - 2009. N2 - The past two decades have witnessed increased scrutiny regarding efficacy and risk of the once unquestioned therapy of red blood cell RBC transfusion. Simultaneously, a variety of changes have been identified within the RBC and storage media during RBC preservation that are correlated with reduced tissue oxygenation and transfusion-associated adverse effects. These alterations are collectively termed the storage lesion and include extensive biochemical, biomechanical, and immunologic changes involving cells of diverse origin. Time-dependent falls is 2,3-diphosphoglycerate, intracellular RBC adenosine triphosphate, and nitric oxide have been shown to impact RBC deformability and delivery of oxygen to the end-organ. The accumulation of biologic response modifiers such as soluble CD40 ligand sCD4oL, lyso-phosphatidylcholine lyso-PC, and Regulated on ...
Pool Section 29 Regulation 3.2(2) AUSTRALIA Patents Act 1990 COMPLETE SPECIFICATION STANDARD PATENT Application Number: Lodged: Invention Title: Buffy coat separator float system and method The following statement is a full de cription of this invention, including the best method of performing it known to us: P111AHAU/1107 BUFFY COAT SEPARATOR FLOAT SYSTEM AND METHOD INCORPORATION BY REFERENCE 10001] Co-pending U.S. application Serial No. 101263,975 (Attorney Docket No. BATZ 2 00007), filed October 3, 2002, is incorporated herein by reference in its entirety. FIELD OF THE INVENTION [0002] The present invention relates generally to density-based fluid separation and, in particular, to an improved sample tube and float design for the separation and axial expansion -of constituent fluid components layered by centrifugation, and a method employing the same. The present invention finds particular application in blood separation and axial expansion of the bdffy coat layers, and will be described with ...
Maturation (MP) or polishing ponds are widely used throughout the world as a final or tertiary wastewater treatment process for effluent quality improvement from secondary biological treatment systems, including FPs. MPs can be expected to reduce total suspended solids (TSS), ammonia, nitrate and phosphorus levels in effluent, but the primary function of MPs in most parts of the world is the removal of pathogenic organisms. A number of mechanisms in MPs are responsible for pathogen reduction, including settlement, natural die off (a function of time and temperature), elevated pH, UV radiation and predation. The design, size and number (usually in series) of MPs are typically based on the desired bacteriological quality of the final effluent. MPs are typically designed at the same depth as facultative ponds (2 to 5 feet (0.7 to 1.5 m)). Retention times in MPs are typically designed at 10 to 14 days for pathogen reduction; however, shorter retention times are required for TSS removal (four to five ...
0027] In an embodiment of the invention, the first and second spray nozzles 34a, 34b are triggered intermittently by an optical or other sensor or output (not shown) that provides a signal indicating the position of the blade 33. In another embodiment, an encoder signal from the slicer is used to establish position of the blade, and thus is used to set the window and timing of the shot. Thus, for example, when the blade 33 is in a first position A so as to expose the face 36 of the log 1, the first spray nozzle 34a is activated for a predetermined burst period. As each slice falls away from the log 1 (e.g., when the blade 33 is in a second position B obscuring the face 36 of the log 1, the second spray nozzle 34b is activated for a second predetermined burst period. When the second burst is applied, the removed slice may be either on the stack in progress or in transit to the stack. Except for the first and last slices in the stack, when each slice contacts the stack in progress, the ...
Mirasol Sulka Yarn - 240 Magic Seaweed - Reviews. Mirasol Sulka Yarn - 240 Magic Seaweed is available now at Jimmy Beans Wool with Free U.S. Flat Rate shipping for orders over $75, $4 U.S. Flat Rate shipping on all other orders!
BETHESDA, Md. & TOKYO--(BUSINESS WIRE)-- Enviva, a global renewable energy company specializing in sustainable wood bioenergy, and Mitsui O.S.K. Lines (MOL), a leading global marine transport group, today announced they have signed a memorandum of understanding agreement to develop and deploy an environmentally friendly bulk carrier. The goal of the agreement is to reduce the greenhouse gas (GHG) emissions in the ocean transportation of sustainable wood pellets. In the initial stage, the partnership will explore the environmental benefits, commercial and operational feasibilities of various technologies. This will include the Wind Challenger, a cargo ship design with a hard sail, which would reduce emissions by harnessing wind energy. MOL have been jointly studying the technology with cross-industrial partners. Enviva is very excited to partner with MOL on this innovative project to reduce greenhouse gas emissions in our supply chain, said Thomas Meth, Executive Vice President, Sales and ...
Functional characteristics and clinical effectiveness of platelet concentrates treated with riboflavin and ultraviolet light in plasma and in platelet additive solution. Ignatova AA, Karpova OV, Trakhtman PE, Rumiantsev SA, Panteleev MA. Vox Sang. 2015 Dec 8. doi: 10.1111/vox.12364. New Infestin-4 Mutants with Increased Selectivity against Factor XIIa. Kolyadko VN, Lushchekina SV, Vuimo TA, Surov SS, Ovsepyan RA, Korneeva VA, Vorobiev II, Orlova NA, Minakhin L, Kuznedelov K, Severinov KV, Ataullakhanov FI, Panteleev MA. PLoS One. 2015 Dec 15;10(12):e0144940. doi: 10.1371/journal.pone.0144940. eCollection 2015. Global/integral assays in hemostasis diagnostics: promises, successes, problems and prospects. Panteleev MA, Hemker HC. Thromb J. 2015 Jan 28;13(1):5. doi: 10.1186/s12959-014-0032-y. eCollection 2015. Procoagulant impact of the plasmapheresis procedure on coagulation state of collected plasma. Surov S, Ovsepyan R, Vysochin I, Kobzeva E, Khvatov V, Panteleev M, Vuimo T. Blood Transfus. 2015 ...
TY - JOUR. T1 - Density-gradient separation for 4-hydroperoxycyclophosphamide purging of autologous bone marrow grafts.. AU - Rowley, S.. AU - Davis, J.. AU - Braine, H.. AU - Jones, R.. AU - Yeager, A.. AU - Saral, R.. AU - Santos, G.. PY - 1990. Y1 - 1990. N2 - We studied density-gradient separation of autologous bone marrow grafts in preparation for ex vivo 4-hydroperoxy-cyclophosphamide (4-HC) purging. A two-step procedure of buffy coat isolation followed by Ficoll-diatrizoate separation was used. Buffy-coat cells were isolated primarily either using the COBE 2991 Blood Cell Processor or Haemonetics 30 Cell Separator. All density-gradient separations were performed using the COBE 2991. The nucleated cell recoveries after buffy-coat isolation were 44.3 +/- 10.4% and 85.1 +/- 10.5% (+/- standard deviation, P less than 0.001) for the Haemonetics 30 and COBE 2991 isolated grafts, respectively. The final nucleated cell recoveries after density-gradient separation for these two buffy-coat ...
with Joyce M. Lee, Mark W. Newman, Achamyeleh Gebremariam, Preciosa Choi, Dana Lewis, Weston Nordgren, John Costik, James Wedding, Benjamin West, Nancy Benovich Gilby, Christopher Hannemann, Ashley Garrity, and Emily Hirschfeld. 2017. Diabetes Technology & Therapeutics. 19(4). doi:10.1089/dia.2016.0312.. Chanca piedra (Phyllanthrus niruri) is a small herb native to tropical regions, such as the Amazon, that has been used for centuries by indigenous peoples to manage diabetes. Chanca piedra helps the body in lowering both blood sugar and cholesterol levels. It also detoxifies and protects the liver, which can become fatty in diabetics.. ...
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As the benefits of additive manufacturing become increasingly apparent, organizations are seeking ways to improve processes for 3D printing. PADT has long been at the forefront of both Additive Manufacturing and simulation, purchasing our first 3D printer...
Next, the link between histone tail modifications and DNA methylation was examined. Publically available PBMC ChIP-Seq data from the ENCODE project were used to identify regions enriched for four distinct histone H3 modifications. Since the methylome of non-pregnant ccf DNA closely resembled that of buffy coat (PBMC), the level of CpG methylation in non-pregnant ccf DNA was then examined within these regions (Figure 1b). In regions enriched for H3K4me3, 89.9% of cytosines showed less than 20% methylation while only 5.2% of unenriched sites were similarly unmethylated. Conversely, 84.9% of CpG sites were methylated (,75%) in H3K9me3 enriched regions compared to 76.3% in unenriched regions. Distinct differences were also observed when comparing H3K4me1 and H3K27me3 enriched regions to corresponding unenriched CpG sites. Taken together, these data suggest a link between particular histone marks and CpG methylation in buffy coat. Comparison of the methylomes of buffy coat and non-pregnant ccf DNA ...
Cerus Corporation is dedicated solely to safeguarding the worlds blood supply and aims to become the preeminent global blood products company. Based in Concord, California, our employees are dedicated to deploying and supplying vital technologies and pathogen-protected blood components for blood centers, hospitals and ultimately patients who rely on safe blood. With the INTERCEPT Blood System, we are focused on protecting patients by delivering the full complement of reliable products and expertise for transfusion medicine. Cerus develops and markets the INTERCEPT Blood System and remains the only company in the blood transfusion space to earn both CE Mark and FDA approval for pathogen reduction of both platelet and plasma components. Cerus currently markets and sells the INTERCEPT Blood System in the United States, Europe, the Commonwealth of Independent States, the Middle East and selected countries in other regions around the world. The INTERCEPT Red Blood Cell system is in clinical ...
Cytochrome P450 monooxygenases (P450s) catalyze oxidation of various substrates using oxygen and NAD(P)H. Plant P450s are involved in the biosynthesis of primary and secondary metabolites performing diverse biological functions. The recent availability of the soybean genome sequence allows us to identify and analyze soybean putative P450s at a genome scale. Co-expression analysis using an available soybean microarray and Illumina sequencing data provides clues for functional annotation of these enzymes. This approach is based on the assumption that genes that have similar expression patterns across a set of conditions may have a functional relationship. We have identified a total number of 332 full-length P450 genes and 378 pseudogenes from the soybean genome. From the full-length sequences, 195 genes belong to A-type, which could be further divided into 20 families. The remaining 137 genes belong to non-A type P450s and are classified into 28 families. A total of 178 probe sets were found to correspond
Substitution of a single Ga-atom or single Ge-atom (GaAl.sub.12 and GeAl.sub.12 respectively) into the center of an aluminum Keggin polycation (Al.sub.13) produces an optimal water-treatment product for neutralization and coagulation of anionic contaminants in water. GaAl.sub.12 consistently shows .about.1 order of magnitude increase in pathogen reduction, compared to Al.sub.13. At a concentration of 2 ppm, GaAl.sub.12 performs equivalently to 40 ppm alum, removing .about.90% of the dissolved organic material. The substituted GaAl.sub.12 product also offers extended shelf-life and consistent performance. We also synthesized a related polyaluminum chloride compound made of pre-hydrolyzed dissolved alumina clusters of [GaO.sub.4Al.sub.12(OH).sub.24(H.sub.2O).sub.12].sup.7+ ...
Improved sound quality and ease-of-use to help you make a clear diagnosis, every time. Reduces background noise an average of 85% with our proprietary Ambient Noise Reduction Technology AND amplifies sounds up to 24 times. Simple and intuitive user interface. The easy-to-read backlit LCD display includes frequency selection, sound level, remaining... ...
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Within the last six-eight years many papers have reported an association between increased levels of particulate (aerodynamic size less than 10 microns in diameter) air pollution and increased daily death rates and illness in major cities in the US, Europe, and South America. There is a need to better characterize the emission rates and chemical composition of particles emitted from vehicles equipped with the latest emission reduction technology. Particle-mass emission rates were measured for four 1995 Ford vehicles driven over three different driving cycles: the Urban Dynamometer Driving Schedule (UDDS), the Highway Fuel Economy Test (HFET), and the USO6 driving cycle. The average particle-mass emission rates were less than 10 mg/mi for any of the driving cycles. The particle-mass emission rate was least for the HFET and about equal for the UDDS and the USO6 cycles. The elemental composition of the particles, determined by X-ray fluorescence, suggests that 10-30% of the particle-mass is ...
The goal of the FDAs guidelines is to help people reduce how much sodium they consume daily. Currently, American adults consume an average of 3,400 mg of sodium per day - almost 50 percent more than is recommended by the FDA and the American Heart Association. Most of that- as much as 65 percent - comes from food purchased in grocery and convenient stores, and 25 percent comes from eating out at restaurants, according to the AHA. The FDA would like to see adults reduce that amount to no more than 2,300 mg of sodium per day, or roughly one teaspoon of salt.. Most children and adolescents also take in more sodium than is recommended. Current data shows that children ages 6 to 10 consume approximately 2,900 mg per day, while teens consume up to 3,700 mg per day. The FDAs guidelines are generously providing companies with specific goals for defining and measuring progress. The companies also have time to learn and utilize new science on sodium reduction technologies. Within two years, the FDA ...
WASHINGTON, D.C. - The Grocery Manufacturers Association (GMA) today released Sodium and Salt: A Guide for Consumers, Policymakers and the Media, a science policy paper that provides policymakers, consumers, journalists and health professionals with current and scientifically accurate information and resources on sodium and salt.. The food industry takes seriously its commitment to develop products that provide choices for consumers interested in managing their intake of salt (sodium chloride) and sodium, said Robert Brackett, chief science officer for GMA. Companies are achieving lower sodium in products through extensive research, reformulation, new salt reduction technology and new product development. Thanks to these industry efforts, today there are more and more sodium- or salt-modified products available nationwide for consumers in the marketplace.. We hope this paper can be a useful resource for policy makers, including the Institute of Medicines committee on Strategies to Reduce ...
After 6 weeks of refrigerated storage, transfusion of autologous red cells to healthy human volunteers increased extravascular hemolysis, saturated serum transferrin, and produced circulating nontransferrin-bound iron. These outcomes, associated with increased risks of harm, provide evidence that the maximal allowable red cell storage duration should be reduced to the minimum sustainable by the blood supply, with 35 days as an attainable goal.. ...
After 6 weeks of refrigerated storage, transfusion of autologous red cells to healthy human volunteers increased extravascular hemolysis, saturated serum transferrin, and produced circulating nontransferrin-bound iron. These outcomes, associated with increased risks of harm, provide evidence that the maximal allowable red cell storage duration should be reduced to the minimum sustainable by the blood supply, with 35 days as an attainable goal.. ...
Further studies are needed to investigate ACC as a possible alternative first-line treatment for focal cartilage injuries in the knee.
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Otto Stöckl Elektroinstallationen GmbH is a specialist in the field of electrical engineering and building technology systems. Originally founded in 1980, the Austrian company, which is based in Vienna, today has a workforce of over 200 employees. The company was looking to homogenise and virtualise its server environment in order to reduce administration requirements and increase redundancy; a new storage and backup strategy was also to be implemented. Fujitsu PRIMERGY RX300 S7 server and VMware as the virtualisation solution plus an ETERNUS DX80 S2 disk storage system and ETERNUS LT20 tape storage system for backup.
Univer Product has inaugurated its upgraded tomato processing plant in the city of Kecskemét, Hungary.. Claimed to be the biggest of its kind in Central Europe and equipped with advanced technology systems, the Kecskemét factory processes fresh tomatoes to manufacture a variety of products such as aseptic tomato concentrates, ketchup, mustard, jams, dressings, and sauces.. The upgrade increased Univers annual raw tomato processing capacity from 120,000t to 140,000t.. More than 40 locals have been employed to oversee tomato processing activities at the plant since it was opened in August. In addition, the plant benefits approximately 350 local people in the agriculture sector by purchasing from domestic suppliers and increasing the local market.. Univer products made at the plant will be delivered to domestic and export markets.. ...
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While underlining the importance of existing biofuel systems, the WBA also calls for intensified efforts to achieve the market introduction of advanced biofuels based on cellulosic feedstocks. Advanced biofuels are vital for the future but commercial production units are yet to appear on the market. This only can happen if governments set up reliable and long-lasting framework condition for investors to offset the very significant capital costs for the first installation of the new technology systems ...
E-Prescribing or electronic-prescribing (e-Rx) is a technology system that comprises of a framework which enables several medical practitioners to write do
I hope someone can answer this question. My Linux system slows down and eventually grinds to a halt. If I can get to slapd in time and shut it down and restart, I can get my system back. Viewing the diagnostics trace in slapd.log, I noted the following message. What triggered this off was running the slapcat command. Did I do something wrong or is this a problem locker = 15815 ====, bdb_cache_find_entry_id( 1 ): 1 (busy) 2 locker = 0 Regards Unix like TeePee no windows, no gates, Apache inside. Paul Clayton Intec Telecom Systems Ph +27 (0) 21 4309000 Fax +27 (0) 21 4309025 Mobile +27(0) 832853403 ******************************************************************************************* ,http://www.intec-telecom-systems.com/, This e-mail and any attachments are confidential and may also be privileged and/or copyright material of Independent Technology Systems Limited (or its affiliated companies). If you are not the intended or authorised recipient of this email or have received it in error, ...
She will identify possible ways to use logistics cost modelling that would improve cost reduction, operations and more efficiencies to enter international markets. And she will analyse the benefits having operations across international markets that will adapt to new market trends for your organisation.. Telenets head of supply chain, Kurt Schilders, will focus on how to develop a better end-to-end supply chain when he delivers the opening address to conference delegates.. He will explain how Telenet integrated with a retail organisation and what types of benefits and challenges were faced. And he will take a look at how to develop a better end to end supply chain by improving the following: sales and operations planning, people, information technology systems, home delivery and urgent delivery and the market department.. The conference is chaired by Johan Kalkoven, senior lecturer for logistics and supply chain management at Amsterdam University of Applied Sciences. It brings supply chain ...
Prior to his current role, Hodges advanced at Smithfield Foods - a $14 billion global food company and the worlds largest pork processor and hog producer. He previously served as senior vice president, fresh pork and industrial sales at Farmland Foods, and led the sales and marketing teams for its Smithfield® and Farmland® brands. His industry expertise includes business development, sales and marketing, retail and foodservice outreach, new equipment and processing specifications management, risk management, feed and grains oversight and developing integrated information and technology systems. ...
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As they sit in the fridge marinading in their own filthy excretions, human erythrocytes mutate into something grotesque, no longer resembling human blood and wholly incapable of carrying oxygen. It is unbelievable that anybody should want to infuse such material into their patients, and concerns have been raised about the safety of using particularly old cells as their near their expiry. In Australia, packed red blood cells can only be stored for a maximum of 42 days (which is slightly conservative - internationally, the maximum PRBC shelf life is 49 days). Surprisingly, international clinical trial data supports the safety of existing practice. CICM SAQs mention storage lesions often, but only Question 29.1 from the first paper of 2010 has actually asked anything directly about them.
Bloodstream infections (BSI) remain a major cause of mortality in patients with malignancies. We present the first report on the microbiological profile of bacteraemia and fungaemia among cancer patients in Ghana. From January 2010 through December 2013, we retrospectively analyzed the spectrum of bloodstream pathogens in cancer patients from Korle-Bu Teaching Hospital, Ghana-focusing on multidrug resistant isolates (MDRs). Overall BSI were confirmed in 22 % (n = 93/453) of total blood cultures. Our data highlights a co-dominance of Gram-negative (n = 49/93, 52.6 %) and Gram-positive (n = 40/93, 43.0 %) bacteria with the former less likely to infect children than adults [odds ratio (OR), 0.56; 95 % confidence interval (CI) 0.14-0.91; p value = 0.027]. Staphylococcus epidermidis was the most isolated bacteria (30.1 %; n = 28/93). About 61 % (n = 25/41) of Enterobacteriaceae isolates were resistant to cefotaxime; a majority (n = 24/25, 96 %) of which were MDRs and mostly susceptible to amikacin and
This is the story of three tracheal transplants, performed by the husband and wife team Thorsten Walles and Heike Mertsching (now Walles), former collaborators of Paolo Macchiarini. My investigation quickly led to the Walles couple setting their lawyer on me, demanding almost €3000 immediately and up to €100,000 later. Update: 2 weeks later, they had…
The E.Z.N.A.® Blood DNA Maxi Kit is specially designed for large scale isolation of genomic DNA. The kit provides a rapid purification of genomic DNA from up to 20 mL whole blood samples. Sample sources include fresh and frozen whole blood treated with common anticoagulants such as citrate, EDTA and heparin. In addition plasma, serum, buffy coat, bone marrow, lymphocytes, platelets, and body fluid samples can also be used. Phenol/chloroform extractions, and time-consuming steps such as precipitation with isopropanol have been eliminated. DNA purified using the E.Z.N.A.® Blood DNA Maxi method is free of contaminants and enzyme inhibitors making it suitable for most downstream applications such as PCR, Southern blotting and restriction enzyme digestion of high-quality total DNA.. ...
Enrichment of human cells from PBMCs. PBMCs were isolated from buffy coats and blood using Ficoll-Paque Premium (GE Healthcare). For the generation of iDCs, PBMCs were resuspended in HBSS (Invitrogen, Thermo Fisher Scientific) containing 5% heat-inactivated fetal calf serum (HIFCS) and incubated for 30 to 45 minutes. Adherent monocytes were cultured in mixed leukocyte culture-conditioned (MLC-conditioned) media (consisting of DMEM supplemented with 4 mg/ml D-glucose, 6 μg/ml folic acid, 3.6 μg/ml l-asparagine, 116 μg/ml l-arginine, 3.7 mg/ml NaHCO3, 10% FCS, 2 mM l-glutamine, 1 mM HEPES, 1 mM sodium pyruvate, 0.1 mM 2-mercaptoethanol, 100 U/ml penicillin, 50 μg/ml streptomycin, and 100 μg/ml neomycin) supplemented with 50 ng/ml granulocyte macrophage-CSF (GM-CSF) and 20 ng/ml IL-4. On day 4, nonadherent monocyte-derived i-DCs were purified by FACS sorting of CD3-CD14-CD56-CD19-CD209+ cells on a BD FACS Vantage SE Diva Option Sorter (BD Biosciences). NK cells were isolated by negative ...
Enrichment of human cells from PBMCs. PBMCs were isolated from buffy coats and blood using Ficoll-Paque Premium (GE Healthcare). For the generation of iDCs, PBMCs were resuspended in HBSS (Invitrogen, Thermo Fisher Scientific) containing 5% heat-inactivated fetal calf serum (HIFCS) and incubated for 30 to 45 minutes. Adherent monocytes were cultured in mixed leukocyte culture-conditioned (MLC-conditioned) media (consisting of DMEM supplemented with 4 mg/ml D-glucose, 6 μg/ml folic acid, 3.6 μg/ml l-asparagine, 116 μg/ml l-arginine, 3.7 mg/ml NaHCO3, 10% FCS, 2 mM l-glutamine, 1 mM HEPES, 1 mM sodium pyruvate, 0.1 mM 2-mercaptoethanol, 100 U/ml penicillin, 50 μg/ml streptomycin, and 100 μg/ml neomycin) supplemented with 50 ng/ml granulocyte macrophage-CSF (GM-CSF) and 20 ng/ml IL-4. On day 4, nonadherent monocyte-derived i-DCs were purified by FACS sorting of CD3-CD14-CD56-CD19-CD209+ cells on a BD FACS Vantage SE Diva Option Sorter (BD Biosciences). NK cells were isolated by negative ...
GENEzol™ Reagent is a phenol, chloroform and guanidine isothiocyanate based scalable solution for extracting high-quality total RNA as well as simultaneous extraction of RNA, DNA and protein from a wide variety of samples such as blood, buffy coat, plasma, serum, cultured cells and tissue.
An experiment is being conducted to identify unique genetic markers related to internal parasitism of small ruminants. Blood, tissue, and fecal samples are currently being collected from select populations of small ruminants with known parasitism history and/or expected progeny differences (EPD) for internal parasitism and growth traits. Genomic DNA will be purified from buffy coat or tissue samples and screened for single nucleotide polymorphisms in candidate genes. Genes will include cytochrome P450 3A family, and prolactin. In addition, plasma will be screened for unique microRNA populations. Identification of unique polymorphisms will be related to fecal egg counts (FEC), FEC EPDs, growth traits, and reproductive success using regression models and analysis of variance. A graduate student has been selected and is currently collecting data and will begin sample analysis in fall 2012. ...
The Würzburg regenerative medicine researchers Heike and Thorsten Walles, joined by their colleague Jan Hansmann, are now hitting back at the evidence I presented, which all clearly suggests that no animal testing was performed on their tracheal transplants made of pig intestine before those were tested on 3 patients, coincidently none of whom is alive today.…
Wright-stained smears or whole blood collected in EDTA purple-top tube (, 8 hrs old). The tube must be at least one quarter full to be acceptable for testing. Capillary specimen collected in EDTA microtube is also acceptable. The tube must contain at least 375 mcL (bottom line) to be acceptable for testing.. ...
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... a layer of mono-nuclear cells called buffy coat (PBMC/MNC), Ficoll-Paque, and erythrocytes & granulocytes which should be ... These products allow blood to be layered on much more quickly without mixing polysaccharide and blood. An example of such a ... This allows the blood sample to be rapidly pipetted onto the insert, avoiding the need for overlaying it directly onto Ficoll- ... Major blood clotting may sometimes occur in the PBMC layer. Ethylene diamine tetra-acetate (EDTA) and heparin are commonly used ...
Buffy-coat preparations of peripheral blood or aspirates from marrow, spleen, lymph nodes, or skin lesions should be spread on ... The visceral form starts with skin ulcers and later presents with fever, low count of red blood cells, and enlarged spleen and ... Additionally, visceral disease can be diagnosed by blood tests. Leishmaniasis can be partly prevented by sleeping under nets ... Amastigotes are seen within blood and spleen monocytes or, less commonly, in circulating neutrophils and in aspirated tissue ...
... a color often used in description of birds Buffy (dog), Russian President Vladimir Putin's dog Buffy coat, a component of blood ... was nicknamed Buffy or Buffie or Buff Buffy (rapper) (born Kim Ju-hyeon, 1995) member of South Korean band MADTOWN Buffy ... the title character in the Buffy the Vampire Slayer media Buffy Gilmore, a main character in Scary Movie Buffy, the sister-in- ... Buffy is a pet form of the female given name Elizabeth. Buffy or Buffie may also refer to: Buffie Carruth (born 1977), American ...
The layer between the red cells and the plasma is referred to as the buffy coat and is sometimes removed to make platelets for ... A blood bank is a center where blood gathered as a result of blood donation is stored and preserved for later use in blood ... Several types of blood transfusion exist: Whole blood, which is blood transfused without separation. Red blood cells or packed ... For blood donation agencies in various countries, see List of blood donation agencies and List of blood donation agencies in ...
... buffy coat (used to make platelets), and red blood cells. The third method is sedimentation: the blood simply sits overnight ... Whole blood is made up of red blood cells, white blood cells, platelets, and blood plasma. It is best within a day of ... Historically, blood was transfused as whole blood without further processing. Most blood banks now split the whole blood into ... Whole blood (WB) is human blood from a standard blood donation. It is used in the treatment of massive bleeding, in exchange ...
Donor lymphocyte (or leukocyte) infusion (DLI) or buffy coat infusion is a form of adoptive immunotherapy used after ... These donated white blood cells contain cells of the immune system that can recognize and destroy cancer cells. The goal of ... Luznik L, Fuchs EJ (2002). "Donor lymphocyte infusions to treat hematologic malignancies in relapse after allogeneic blood or ...
... "buffy coat" layer, which includes the platelets and the white blood cells. The "buffy coat" is isolated in a sterile bag, ... American Association of Blood Banks (2003). "5.1.5.1". Standards for Blood Banks and Transfusion Services (22nd ed.). Bethesda ... On a stained blood smear, platelets appear as dark purple spots, about 20% the diameter of red blood cells. The smear is used ... Although thrombosis, blood coagulation in intact blood vessels, is usually viewed as a pathological immune response, leading to ...
... "buffy coat" layer, which includes the platelets and the white blood cells. The "buffy coat" is isolated in a sterile bag, ... American Association of Blood Banks (2003). "5.1.5.1". Standards for Blood Banks and Transfusion Services (22nd ed.). Bethesda ... Platelets, also called thrombocytes (thromb- + -cyte, "blood clot cell"), are a component of blood whose function (along with ... On a stained blood smear, platelets appear as dark purple spots, about 20% the diameter of red blood cells. The smear is used ...
Platelet-rich plasma (PRP) is widely used in North America and Buffy coat (BC) is more widely used in Europe. Platelets are the ... Since it takes up to 3 liters of whole blood (the amount of a dozen blood bags) to generate a dose of platelets, white blood ... Blood accounts for about 8% of body weight, so a 50 kg (110 lb) donor has about four liters of blood. No more than 50% of a ... Even though red blood cells can also be collected in the process, most blood donation organizations do not do so because it ...
They have a short thin coat which is usually a grayish beige color, "... a mixture of black and slightly buffy grey" according ... ISBN 978-0-89658-640-6. Mills, Clarence A. (1945). "Influence of Environmental Temperatures on Warm-Blooded Animals". Annals of ... It is distinguished from the Indian wolf by its smaller skull, smaller size and thinner coat. Arabian wolves are mainly ...
It can be detected by microscopic examination of fresh anticoagulated blood, or its buffy coat, for motile parasites; or by ... Screening of donated blood, blood components, and solid organ donors, as well as donors of cells, tissues, and cell and tissue ... T. cruzi can also be transmitted through blood transfusions. With the exception of blood derivatives (such as fractionated ... blood supply.[38][39] The Chagas Biovigilance Network reports current incidents of Chagas-positive blood products in the United ...
White cells are found in the buffy coat, a thin, typically white layer of nucleated cells between the sedimented red blood ... All white blood cells have nuclei, which distinguishes them from the other blood cells, the anucleated red blood cells (RBCs) ... Histamine is responsible for widening blood vessels and increasing the flow of blood to injured tissue. It also makes blood ... "Blood. 96 (13): 4028-38. PMID 11110670.. *^ a b c d e f Kumar V, et al. (2010). Robbins and Cotran pathologic basis of disease ...
White cells are found in the buffy coat, a thin, typically white layer of nucleated cells between the sedimented red blood ... The complete blood cell count is a blood panel that includes the overall WBC count and the white blood cell differential, a ... All white blood cells have nuclei, which distinguishes them from the other blood cells, the anucleated red blood cells (RBCs) ... Histamine is responsible for widening blood vessels and increasing the flow of blood to injured tissue. It also makes blood ...
The layer between the red cells and the plasma is referred to as the buffy coat and is sometimes removed to make platelets for ... A blood bank is a center where blood gathered as a result of blood donation is stored and preserved for later use in blood ... Types of blood transfusedEdit. Whole blood or blood with RBC, is transfused to patients with anaemia/iron deficiency. It also ... For blood donation agencies in various countries, see List of blood donation agencies and List of blood donation agencies in ...
... mayayakit la reng mangaputing cell king Buffy coat, metung a maimpis a tumpak (layer) da reng cell king pilatan da reng ... Blood values. Diameter (μm)[3]. Main targets[4]. Nucleus[4]. Granules[4]. Lifetime[3] ... Melalang ya ing katayang "korpuskulos a maputi" o "White Blood Cbgfgmjdghfell" uling kaibat ning centrifugation ning daya, ...
Diana calls out for Michelle, who hid from Grace, and finishes the blood-spiked cup of tea. But Michelle has slipped away, and ... "what a fresh coat of paint can really do." Behind the scenes, however, the change led to shifts in the shooting schedule, ... apparent debt to such earlier fare as Dark Shadows and Buffy the Vampire Slayer. These sentiments were echoed by the gay- ... Diana excuses herself to find more scrolls, and Michelle takes the opportunity to slip a few drops of her own blood into ...
... using the part-fairy blood of Danielle's son Jakub - Danielle having some fairy blood in her from her mother's side of the ... Before declaring Sidney free, the Snow Queen advises Sidney to get a warm coat since it is "going to get cooler around here." ... voiced by Buffy Sainte-Marie) is a shiny flat rock. It told Sly Fox that it was the fairest in the land until White Snow came ...
The Buffy alumna was cast into the role of Georgina Sparks, described by Michael Ausiello as "an adolescent Cruella". Creator ... The third-season finale has Georgina return from Belarus wearing a blond wig and large coat, desperately seeking the help of ... as well as a drug addict who has blood red lips and long glossy dark hair, meets Nate Archibald in rehab. The two date for a ... Ausiello, Michael (19 March 2008). "Gossip Girl casts Buffy alumna Michelle Trachtenberg". Ausiello Report, TVGuide.com. ...
A teacher had inspired him to write whatever he wanted, so he wrote a story that featured blood and mutilation and read it ... The army ambush her with nets and sticks, and surround her tightly as Colonel Buffy announces that they had come to arrest her ... Because she could not find her old clothes, she improvised with bath towels, a fishing hat, wellington boots and a fur coat, ... And there was so much blood!" After Royds' suggestion, he realised as he wrote more stories that "what I was writing was a ...
They are smallish birds with tails that are usually long and pointed, and tend to be drab brownish or buffy all over. Striated ... Gallus gallus Blood pheasant, Ithaginis cruentus Himalayan monal, Lophophorus impejanus Snow partridge, Lerwa lerwa Satyr ... and feathers that are excellent at shedding water due to an oily coating. Fulvous whistling-duck, Dendrocygna bicolor (A) ...
They are smallish birds with tails that are usually long and pointed, and tend to be drab brownish or buffy all over. Fly River ... and feathers that are excellent at shedding water due to an oily coating. Spotted whistling-duck, Dendrocygna guttata Plumed ... Blood-breasted flowerpecker, Dicaeum sanguinolentum (E) Mistletoebird, Dicaeum hirundinaceum Scarlet-backed flowerpecker, ... Bubo sumatranus Buffy fish-owl, Ketupa ketupu Collared owlet, Glaucidium brodiei Javan owlet, Glaucidium castanopterum (E) ...
According to his daughter, he usually "sweated blood" to write his songs.[45] Annie Get Your Gun is considered to be Berlin's ... "All the little writer could do was to finger the buttons on his coat while tears ran down his cheeks-in a vaudeville house!"[20 ... Music: Jack Nitzsche and Buffy Sainte-Marie. *Lyrics: Will Jennings (1982). *"Flashdance... What a Feeling" *Music: Giorgio ...
Buffy coat is the component of whole blood that contains leukocytes an...,Genomic,DNA,Extraction,from,Buffy,Coat,Using,the, ... Perfect,gDNA,Blood,,,,,,,,,,,,Mini,Kit,biological,advanced biology technology,biology laboratory technology,biology device ... The versatile Perfect gDNA Blood Mini Kit allows the researcher to iso... ... Laura Pollock ...The Perfect gDNA Blood Mini Kit is an efficient way to purify genomic ... Introduction ... ...
BUFFY COAT (HG-BCD) reagent with the ReliaPrep™ Blood gDNA Miniprep System. ... we examine the compatibility of buffy coat DNA preserved in HEMAgene™• ... HEMAgene™•BUFFY COAT (HG-BCD) Preservative Compatibility with ReliaPrep™ Blood gDNA Miniprep System Application Note. ... HEMAgene BUFFY COAT HG BCD Preservative Compatibility with ReliaPrep Blood gDNA Miniprep System AN281PDF. (. 342. KB. ). - ...
Comparative Study of Modified Quantitative Buffy Coat and Two Rapid Tests in Comparison with Peripheral Blood Smear in Malaria ... and modified QBC was done in comparison with conventional blood smear microscopy. One hundred patients infected with P. vivax ...
The double dose buffy coat method requires fewer buffy coats and reduces the use of consumables by up to 50% (e.g. pooling sets ... INTERCEPT Blood System, amotosalen, UVA, platelet, blood processing, buffy coat, IBS, transfusion HSV kategori Identifikatorer ... Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System. Abedi, ... is prepared by pooling the buffy coat layer from up to six whole blood donations. A procedure for producing "double dose" whole ...
This BCTB blood sample subcollection consists of buffy-coat samples. A buffy coat contains white blood cells and platelets. ... This BCTB blood sample subcollection consists of buffy-coat samples. A buffy coat contains white blood cells and platelets.&rft ... BCTB Blood Buffy Coat Samples&rft.identifier=abctb.org.au 13&rft.publisher=Breast Cancer Tissue Bank&rft.description= ...
We compared this technique with the thick blood smear diagnosis in a study of the efficacy of chloroquine therapy in Zaire. A ... Recently, a new direct diagnosis technique, the Quantitative Buffy Coat (QBC®) * Malaria Diagnosis System, has been developed. ... Chloroquine resistance was detected in 38% of the subjects by thick blood smear and in 45% by the QBC test. Of greater interest ... an average of 17 min was required to examine microscopic fields with 1,000 leukocytes by thick blood smear analysis compared ...
... whereas the number of positive readings assessed by means of stained blood films fell significantly at dilutions below 3,263 ... In the present study, we used acridine orange-coated quantitative buffy coat (QBC) tubes, centrifugation, and fluorescence ... louse-borne relapsing fever is commonly made on the basis of the detection of Borrelia spirochetes on Giemsa-stained thin blood ... In the present study, we used acridine orange-coated quantitative buffy coat (QBC) tubes, centrifugation, and fluorescence ...
The quantitative buffy coat (QBC) is a technique that is as sensitive as thick smears. Because results cannot be used to ... These red blood cells (RBCs) are sequestered in the spleen when malaria proteins, called sequestrins, on the RBC surface bind ... The quantitative buffy coat (QBC) is a technique that is as sensitive as thick smears. Because results cannot be used to ... What is the role of the quantitative buffy coat (QBC) technique in the diagnosis of malaria?. Updated: Apr 26, 2019 ...
Whole Blood Columns and Whole Blood Column Elution Buffer are included in the kit to allow direct processing of an entire buffy ... Buffy Coat CD4 MicroBead Kit has been developed for the rapid positive selection of CD4+ cells directly from one buffy coat ... One entire buffy coat is labeled with StraightFrom. ® Buffy Coat CD4 MicroBeads and loaded onto Whole Blood Columns, which are ... One entire buffy coat is labeled with StraightFrom. ® Buffy Coat CD4 MicroBeads and loaded onto Whole Blood Columns, which are ...
Whole Blood Columns and Whole Blood Column Elution Buffer are included in the kit to allow direct processing of an entire buffy ... Buffy Coat CD56 MicroBead Kit has been developed for the rapid positive selection of CD56+ cells directly from one buffy coat ... One entire buffy coat is labeled with StraightFrom™ Buffy Coat CD56 MicroBeads and loaded onto Whole Blood Columns, which are ... Separation of a buffy coat sample using the StraightFrom™ Buffy Coat CD56 MicroBead Kit and the MultiMACS™ Cell24 Separator ...
... tested and described the buffy coat method (BCM) for quick diagnostics (~ 20 min/sample) of avian blood parasites. Blood of 345 ... and each sample was examined using ME of stained blood films and the buffy coat, which was examined after centrifugation in ... BCM is a useful tool for diagnostics of blood parasite co-infections. Its application might be extended to studies of blood ... However, fixation, drying and staining of blood films as well as their ME are relatively time-consuming. This limits the ...
... automated method for extraction of genomic DNA from 50-250µl of buffy coat samples using the Maxwell RSC Instrument. ... The Maxwell RSC Buffy Coat DNA Kit is a simple, ... Maxwell® RSC Whole Blood DNA Kit. Extracts DNA from whole blood ... The Maxwell® RSC Buffy Coat DNA Kit is a simple, automated method for extraction of genomic DNA from 50-250µl of buffy coat ... Automated DNA Purification from Buffy Coat samples. *Purifies high yields of DNA from 50-250µl of buffy coat samples in about ...
Whole blood. (anticoagulated or blood clots). Buffy coat. 100 - 400 μg/10 mLc. ~200 μg/mLc - relatively low-cost storage (-80° ... Blood Spots. 12 - 42 ng/μL (adults)a. 43 - 78 ng/μL (neonates)a. 1/4 inch punch from 75 μL volume yields about 12 μL of bloodb ... with current technology as little as 2.5 ng per SNP so that scores to hundreds of genotypes could be obtained from one blood ...
Compared to blood culture, the overall sensitivity of acridine orange stained buffy coat smears was 16%, specificity 88%, and ... A total of 356 consecutive blood cultures were submitted with simultaneous anticoagulated blood samples, from which a buffy ... Rapid Diagnosis of Bacteremia in Adults Using Acridine Orange Stained Buffy Coat Smears. Mark Miller1,2 and Jack Mendelson1 ... The use of acridine orange stained buffy coat smears was assessed as a rapid screening test for bacteremia in adults. ...
HLA Chimerism Buffy Coat CD56 Positive Selection Kit II is designed to isolate CD56+ cells with an EasySep™ magnet from freshly ... EasySep™ HLA Chimerism Buffy Coat CD56 Positive Selection Kit (Catalog #17875). *EasySep™ HLA Chimerism Buffy Coat CD56 ... This product replaces the EasySep™ Human Buffy Coat CD56 Positive Selection Kit (Catalog #18085) and EasySep™ HLA Buffy Coat ... HLA Chimerism Buffy Coat CD56 Positive Selection Kit II is designed to isolate CD56+ cells from freshly prepared buffy coat by ...
Preparation of buffy coat. *Centrifuge the blood specimen at 2,500 x g for 10 minutes at room temperature. ... Erythrocytes can be removed through the use of a hypotonic buffer or by using gradient centrifugation to create a buffy coat in ... The light tan middle layer is the buffy coat and contains concentrated leukocytes. The bottom red layer contains erythrocytes. ... Removal of eukaryotes from bloodIf DNA is being extracted from clinical specimens such as blood, CSF, or serum, considerations ...
The morphology of buffy coat from cord blood of normal human newborns was investigated and the results were compared with our ... P. EFRATI, L. ROZENSZAJN, E. SHAPIRA; The Morphology of Buffy Coat from Cord Blood of Normal Human Newborns. Blood 1961; 17 (4 ... The Morphology of Buffy Coat from Cord Blood of Normal Human Newborns P. EFRATI, P. EFRATI ... The morphology of buffy coat from cord blood of normal human newborns was investigated and the results were compared with our ...
... rich plasma and buffy coats using a new technique with 111 In and 51 Cr, Transfusion" on DeepDyve, the largest online rental ... 70% of whole blood). WBCs remaining in the BC‐PC averaged 0.19 × 108 per unit compared to 3.6 × 108 per unit for PRP‐PC. Buffy ... 70% of whole blood). WBCs remaining in the BC‐PC averaged 0.19 × 108 per unit compared to 3.6 × 108 per unit for PRP‐PC. Buffy ... Paired comparison of platelet concentrates prepared from platelet‐rich plasma and buffy coats... Keegan, T.; Heaton, A.; Holme ...
Blood. *EDTA plasma. *DSMO (10%) whole blood. *Buffy coat. *Peripheral blood mononuclear cells ... Step 2: A general inventory of blood and cardiovascular-related tissues will be posted on the Cardiovascular Centers InfoScope ...
Blood Plasma. The Buffy Coat. 55% 45%. Blood Plasma. Cellular Components. The Cellular Components. Blood Facts. Blood makes up ... The buffy coat is made up of white blood cells and platelets.. This makes up less than 1% of the total blood volume.. There are ... Buffy Coat. Platelets and INR. eGFR and Creatinine. Blood plasma is the straw coloured liquid component of blood.. 91% is water ... and therefore AB plasma is given to patients with any Blood type. Human Blood; red Blood cells, white Blood cells, plasma and ...
... red blood cells (RBC), buffy coat, plasma, serum, DNA and RNA that derived from blood in human biobanks. These biological ... Repository of Human Blood Derivative Biospecimens in Biobank: Technical Implications.. Mohamadkhani A1, Poustchi H1. ... This review overview the significance and storage condition of biospecimens including whole blood, ...
Leukocyte count in association with whole blood culture, whole blood PCR, buffy coat culture and buffy coat PCR - The average ... In conclusion, buffy coat PCR, buffy coat culture and whole blood PCR can be used as alternative methods to whole blood culture ... Comparison of buffy coat PCR with whole blood culture - The buffy coat PCR showed that 47 of the 114 specimens (or 41.2%) were ... whole blood culture and buffy coat PCR, buffy coat culture or whole blood PCR. A Kappa coefficient of 0.8-1.0 indicates a very ...
The method comprises combining the blood 5 sample with one or more labelling agents so as to differentiate target cells from ... After the centrifuging, the blood sample present in the clearance gap is examined to identify any target cells contained ... The method further includes centrifuging the blood sample and separator float in the sample 15 tube to effect centrifugally ... other cells in the blood sample; introducing the blood sample into a transparent sample tube comprising a flexible side wall ...
detection, Giemsa-stained blood smear. +§. +§. +§. +¶. Quantitative buffy coat. ND. ND. ND. +. ELISA anti-B. murgdorferi (titer ... First sample was positive after review prompted by the quantitative buffy coat result.. #Siemens, Erlangen, Germany.. **Bio- ...
Here we validated a novel procedure for ambient temperature preservation of DNA in white blood cell and buffy coat lysates ... High DNA stability in white blood cells and buffy coat lysates stored at ambient temperature under anoxic and anhydrous ... High DNA stability in white blood cells and buffy coat lysates stored at ambient temperature under anoxic and anhydrous ... High DNA stability in white blood cells and buffy coat lysates stored at ambient temperature under anoxic and anhydrous ...
quantitative buffy coat capillary tubes versus thin and thick blood films in the diagnosis of malaria in Saudi Arabia ... The, quantitative buffy coat capillary tubes versus thin and thick blood films in the dia ... The quantitative buffy coat [QBC] capillary tube was compared with both the thin and thick films in the diagnosis of malaria ... Index: IMEMR (Eastern Mediterranean) Main subject: Blood Chemical Analysis / Laboratories Limits: Humans Language: English ...
Present study was undertaken to compare and study the efficacy of peripheral blood smear (PBS), modified centrifuged buffy coat ... Key words Malaria, Peripheral blood smear, centrifuged buffy coat smear, Rapid malaria antigen detection test ... Present study was undertaken to compare and study the efficacy of peripheral blood smear (PBS), modified centrifuged buffy coat ... A Prospective Comparative Study of Peripheral Blood Smear, Modified Centrifuged Buffy Coat Smear and Rapid Malaria Antigen ...
Ultrastructural study of human umbilical cord blood Buffy coat and Whartons Jelly cells ... Ultrastructural study of human umbilical cord blood Buffy coat and Whartons Jelly cells ... the UCB buffy coat cells showed immature cellular constituents and few changes in the ultrastructural morphology of cord blood ... Tiny specimens from the UCB buffy coat and the WJ were double-fixed with glutaraldehyde and osmium tetroxide, dehydrated, and ...
Granulocyte transfusions - directed donation and buffy coats. This clinical guideline explains the process at Great Ormond ... Blood tests: requesting, labelling and sampling requirements. The purpose of this guideline is to provide guidance about blood ... Manual exchange blood transfusion protocol. This guidance for health professionals from Great Ormond Street Hospital (GOSH) is ... Cell salvage blood (intraoperative), administration in a non-theatre setting. This information from Great Ormond Street ...
  • Platelets , also called thrombocytes (from Greek θρόμβος, "clot" and κύτος, "cell"), are a component of blood whose function (along with the coagulation factors ) is to react to bleeding from blood vessel injury by clumping, thereby initiating a blood clot . (wikipedia.org)
  • The platelets congregate around the wound in order to create a cap to stop blood flow out of the tissue. (wikipedia.org)
  • On a stained blood smear , platelets appear as dark purple spots, about 20% the diameter of red blood cells. (wikipedia.org)
  • The ratio of platelets to red blood cells in a healthy adult ranges from 1:10 to 1:20. (wikipedia.org)
  • Platelets , also called thrombocytes ( thromb- + -cyte , "blood clot cell"), are a component of blood whose function (along with the coagulation factors ) is to stop bleeding by clumping and clotting blood vessel injuries. (wikipedia.org)
  • [12] Max Schultze in 1865 described what he called "spherules", which he noted were much smaller than red blood cells, occasionally clumped, and were sometimes found in collections of fibrin material. (wikipedia.org)
  • Buffy Coat CD4 MicroBead Kit and the MultiMACS™ Cell24 Separator Plus with the Single-Column Adapter and Whole Blood Columns. (miltenyibiotec.com)
  • Whole Blood Columns and Whole Blood Column Elution Buffer are included in the kit to allow direct processing of an entire buffy coat. (miltenyibiotec.com)
  • An arterial thrombus may partially obstruct blood flow, causing downstream ischemia , or may completely obstruct it, causing downstream tissue death . (wikipedia.org)
  • Platelet concentration is measured either manually using a hemocytometer , or by placing blood in an automated platelet analyzer using electrical impedance , such as a Coulter counter . (wikipedia.org)
  • DNA from 17 chimpanzees (lung, spleen, or liver), form a separate novel genus, with the proposed name of 30 red colobus monkeys (buffy coat, blood, bone marrow, Partetravirus, within the subfamily Parvovirinae . (cdc.gov)
  • These ultrastructural changes may clarify the specific cultural, immunological and regenerative properties attributed to cord blood in comparison to bone marrow or peripheral blood . (bvsalud.org)
  • It is characterized by the accumulation of CD5/CD19/CD23 positive B-lymphocytes in the blood, bone marrow, lymph nodes and spleen. (nature.com)
  • Complete or nearly complete absence of the granular leukocytes from the blood and bone marrow. (free-ed.net)
  • A fatal condition of the blood-forming tissues, characterized by marked proliferation of immature cells in the bone marrow, without their presence, in any great numbers, in the blood steam. (free-ed.net)
  • MP Biomedicals offers three products for the isolation of mononuclear and polymorphonuclear cells from human peripheral blood as well as from bone marrow and umbilical cord blood. (genengnews.com)
  • LSM allows for the separation of lymphocytes not only from human peripheral blood, but also from bone marrow as well as umbilical cord blood. (genengnews.com)
  • A major obstacle to curing metastatic neuroblastoma is the presence of minimal residual disease (MRD) in the bone marrow and peripheral blood even after the patient has achieved clinical remission. (aacrjournals.org)
  • This review overview the significance and storage condition of biospecimens including whole blood, red blood cells (RBC), buffy coat, plasma, serum, DNA and RNA that derived from blood in human biobanks. (nih.gov)
  • Using real-time quantitative methylation-specific PCR (RTQ-MSP), we quantified methylated p16INK4a sequences and determined the fractional concentrations of circulating tumor DNA in plasma, serum, and peripheral blood cells collected preoperatively, intraoperatively, and postoperatively from 49 patients with hepatocellular carcinoma (HCC). (aacrjournals.org)
  • In this investigation, we measured concentrations of tumor-derived methylated p16INK4a sequences in plasma, serum, and buffy coat collected from HCC patients before, during, and after surgical resection. (aacrjournals.org)
  • The devices are employed in novel apparatus of the invention, for the collection, separation and filtration of blood serum and blood. (google.com)
  • The devices are employed in novel apparatus of the invention, for the collection, separation and filtration of blood serum and blood plasma from clotted whole blood and anti-coagulant treated whole blood, respectively. (google.com)
  • The NucleoSpin 8/96 Blood QuickPure kits are designed for the rapid parallel isolation of genomic DNA from whole blood, serum, plasma, and body fluids (amniotic fluid, liquor, etc.)-in flexible 8-well strip or high-throughput 96-well plate formats which have been optimized for manual use. (clontech.com)
  • Fractionation of white blood cells (WBCs), aka leukocytes, offers several advantages over use of whole blood, including concentration of the sample, removal of the RNases found in plasma, and the ability to use a more homogeneous population of cells for analysis. (thermofisher.com)
  • Blood fractionation to obtain the "buffy coat" fraction containing the WBCs involves centrifuging anticoagulated blood to separate the cell components according to density, allowing recovery of the total leukocyte population. (thermofisher.com)
  • The extent to which centrifugation affects mRNA profiles in WBCs recovered in the buffy coat fraction is a concern. (thermofisher.com)
  • 1. Hematocrit Your answer : a. refers to the percentage of white blood cells (WBCs) in a sample of whole blood. (bartleby.com)
  • In this study, we examined the levels of the normal, cellular prion protein (PrPC), and the disease-causing isoform (PrPSc) in subpopulations of circulating white blood cells (WBCs) from patients with sporadic (s) CJD, age-matched neurological controls and healthy donors. (nih.gov)
  • Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD16, CD19, CD36, CD38, CD45, CD66b and glycophorin A on red blood cells (RBCs). (stemcell.com)
  • The antibody cocktail crosslinks unwanted cells to red blood cells (RBCs), forming rosettes. (stemcell.com)
  • red blood cells ( RBCs or erythrocytes ). (getbodysmart.com)
  • Red blood cells (RBCs) in normal human blood tend to form linear and branched aggregates. (spiedigitallibrary.org)
  • Correct answer: b. of 40 means that 40% of the volume of blood consists of RBCs. (bartleby.com)
  • The DNA Isolation Kit for Mammalian Blood rapidly isolates DNA from 1 to 10 ml mammalian whole blood, lymphocytes, or buffy coat samples. (sigmaaldrich.com)
  • We report here the first near-field IR imaging of lymphocyte nuclei, and far-field IR imaging results of whole lymphocytes and nuclei from normal human blood. (rsc.org)
  • The separation principle used by LSM to isolate lymphocytes from the other blood constituents is summarized in Figure 1 . (genengnews.com)
  • 2 Transmission typically follows exposure to infected lymphocytes in blood, or through breastfeeding or sexual intercourse. (mja.com.au)
  • Umbilical cord blood [UCB] was used in the treatment of several hematological diseases . (bvsalud.org)
  • Short report: Detection of borrelia (relapsing fever) in rural Ethiopia by means of the quantitative buffy coat technique. (ajtmh.org)
  • Several tubes of blood were collected from a healthy volunteer into evacuated tubes containing EDTA anticoagulant (Terumo). (thermofisher.com)
  • The ChargeSwitch ® gDNA Blood Kits are designed to allow isolation of genomic DNA from the following amounts of fresh or frozen, human blood treated with the anticoagulant EDTA or citrate. (thermofisher.com)
  • Temporary cessation of venous blood flow. (studystack.com)
  • The temporary cessation or slowing of the venous blood flow. (studystack.com)
  • therefore, the buffy coat from venous blood is an adequate substitute. (medscape.com)
  • If the equipment for buffy coat is unavailable, an untreated venous blood sample is left to clot (from 20-120 minutes) and the plasma removed. (medscape.com)
  • The time required for venous blood, in the absence of all tissue factors, to clot in glass tubes under controlled conditions. (free-ed.net)
  • Specimens that do not yield any culture can still be analyzed by molecular methods (see below) that can be applied on DNA extracted from clinical specimens (typically, blood and CSF). (cdc.gov)
  • Tiny specimens from the UCB buffy coat and the WJ were double-fixed with glutaraldehyde and osmium tetroxide , dehydrated, and embedded in epoxy resin. (bvsalud.org)
  • A health care professional trained to collect blood specimens. (studystack.com)
  • This study seeks to confidentially collect blood from HIV-positive individuals and HIV-negative controls to provide basic scientists with specimens for collaborative studies. (clinicaltrials.gov)
  • There are numerous requests from basic scientists at UCSF for collaborative studies on specimens, especially for blood, saliva and urine samples. (clinicaltrials.gov)
  • Tumor specimens from two (n=4) or three (n=4) tumor sites and blood were obtained with one month. (aacrjournals.org)
  • Solutions for oxygen transport are being developed and fibrinogen coated microcapsules are being investigated for thrombocytopenia. (springer.com)
  • Recently, a new direct diagnosis technique, the Quantitative Buffy Coat (QBC®) * Malaria Diagnosis System, has been developed. (ajtmh.org)
  • What is the role of the quantitative buffy coat (QBC) technique in the diagnosis of malaria? (medscape.com)
  • The quantitative buffy coat [QBC] capillary tube was compared with both the thin and thick films in the diagnosis of malaria among 3 groups of hospitalized patients . (bvsalud.org)
  • In Burkina Faso, malaria treatment is prescribed after positive malaria rapid diagnostic test (RDT) or thick blood smears confirmation regardless of the nutritional status of the child. (academicjournals.org)
  • Blood of 345 birds belonging to 42 species was collected, and each sample was examined using ME of stained blood films and the buffy coat, which was examined after centrifugation in capillary tubes and after being transferred to objective glass slides. (biomedcentral.com)
  • The panel of mRNAs amplified was chosen to include genes whose expression in previous studies was shown to be especially volatile in blood (e.g., p53 tumor suppressor gene, cytokine IL-8, and c-jun oncogene). (thermofisher.com)
  • isolated monocytes from the peripheral blood of axial spondyloarthritis patients and healthy controls to study differential tumor necrosis factor alpha-induced protein 3 (TNFAIP3) regulation in blood-derived macrophages. (genengnews.com)
  • Tumor mutations were identified by comparison to buffy coat DNA sequences. (aacrjournals.org)
  • DNA Isolation Kit for Mammalian Blood has been used in isolation of genomic DNA from blood of asthmatic patients for single nucleotide polymorphisms genotyping. (sigmaaldrich.com)
  • The ChargeSwitch ® gDNA Purification Kits allow rapid and efficient purification of genomic DNA from small volumes of human blood. (thermofisher.com)
  • ChargeSwitch ® gDNA 20 µl Blood Kits: Purifies up to 600 ng of genomic DNA from 10-20 µl of human blood. (thermofisher.com)
  • ChargeSwitch ® gDNA 100 µl Blood Kit: Purifies up to 3 µg of genomic DNA from 50-100 µl of human blood. (thermofisher.com)
  • The diagnosis of louse-borne relapsing fever is commonly made on the basis of the detection of Borrelia spirochetes on Giemsa-stained thin blood films. (ajtmh.org)
  • Microscopical examination (ME) of stained blood films remains the gold standard method for the detection of these infections in birds, particularly because co-infections predominate in wildlife. (biomedcentral.com)
  • Unlike blood culture, nucleic acid-based assays do not require growth of an organism for detection, are proven sensitive in diagnosing nonculturable pathogens and have a shorter turnaround time (Greisen et al. (fiocruz.br)
  • Quantification of epigenetic changes in peripheral blood by RTQ-MSP is useful for the detection and monitoring of HCC. (aacrjournals.org)
  • The invention enables non-invasive prenatal diagnosis including for example sex determination, blood typing and other genotyping, and detection of pre-eclampsia in the mother. (google.ca)
  • The quantitative buffy coat (QBC) is a technique that is as sensitive as thick smears. (medscape.com)
  • The use of acridine orange stained buffy coat smears was assessed as a rapid screening test for bacteremia in adults. (hindawi.com)
  • Direct smears from cord blood were examined. (ashpublications.org)
  • Although separating the blood into its components requires an additional step, the benefits of higher gDNA concentration and yield out-weigh the extra effort. (bio-medicine.org)
  • How do you store different blood components and for how long? (brainscape.com)
  • Increase in the concentration of the non-filterable blood components. (studystack.com)
  • These programs allow our blood center to ship and/or receive blood components to maintain optimal blood supplies at all times. (ribc.org)
  • Approximately 1% of the blood volume is occupied by these components. (getbodysmart.com)
  • In clinical medicine conventional blood and its components are used in supportive therapies dependent on the choice of apparent uncritical trigger factors. (springer.com)
  • This product replaces the EasySep™ Human Buffy Coat CD56 Positive Selection Kit (Catalog #18085) and EasySep™ HLA Buffy Coat CD56 Positive Selection Kit (Catalog #18085HLA), for even faster cell isolations. (stemcell.com)
  • The morphology of buffy coat from cord blood of normal human newborns was investigated and the results were compared with our previous observations in adults. (ashpublications.org)
  • Repository of Human Blood Derivative Biospecimens in Biobank: Technical Implications. (nih.gov)
  • The outstanding landmark in the history of blood transfusion is the discovery of human ABO blood group system in 1900 by Landsteiner. (hubpages.com)
  • He differentiated human blood into four distinct groups on the basis of two antigens A and B . (hubpages.com)
  • Alternative approaches include the use of cytokines, growth factors, humanised monoclonal antibodies, recombinant plasma factors, and buffy coat derived natural human interferons. (springer.com)
  • Buffy coat is stored frozen and DNA is extracted from stored buffy coat when it is requested for an ancillary study. (whi.org)
  • New potential uses of cord blood in nonhematopoietic applications are now proposed. (bvsalud.org)
  • The degree of hypoxia was ascertained by measurement of Hyp and Xan concentrations in cord blood. (thefreelibrary.com)
  • Universal primers targeting the 16S rRNA gene in bacteria have been used in the identification of a wide range of bacterial pathogens in blood, cerebrospinal fluid (CSF) and other normally sterile body fluids (Klausegger et al. (fiocruz.br)
  • A general inventory of blood and cardiovascular-related tissues will be posted on the Cardiovascular Center's InfoScope webpage and/or is available via email ([email protected]) for investigators prior to application. (mcw.edu)
  • The term vial refers to the tubes typically used for collection of the blood. (bio-medicine.org)
  • Note that some red blood cell trapping (presence of erythrocytes & granulocytes) may occur in the PBMC or Ficoll-Paque layer. (wikipedia.org)
  • Major blood clotting may sometimes occur in the PBMC layer. (wikipedia.org)
  • What is used to replace missing or low levels of proteins in blood? (brainscape.com)
  • The safety of blood and the quantification of transfusion risks have been dominant themes that have stimulated the development of alternative approaches in this rapidly developing area. (springer.com)
  • Blood centers are faced with many challenges including maximizing production yield from the blood product donations they receive as well as ensuring the highest possible level of safety for transfusion patients, including protection from transfusion transmitted diseases. (diva-portal.org)
  • Type AB plasma has been considered as the universal Blood plasma type, and therefore AB plasma is given to patients with any Blood type. (prezi.com)
  • To address this issue, we sorted DC precursors from the peripheral blood of CLL patients and found a profoundly altered compartment as compared with normal donors. (aacrjournals.org)
  • Eight different WBC fraction types from the peripheral blood of patients with sCJD were assessed for PrPSc. (nih.gov)
  • Nucleic acids extracted from MPure Blood DNA Extraction Kit 200 can be used in a number of downstream application including: PCR, qPCR, Sequencing (NGS), Microarray, RFLP, Southern Blot Analysis. (mpbio.com)
  • Please note that DNA is extracted on an 'as needed' basis so the date of extraction does not correlate with the date of the blood draw. (whi.org)
  • Separating blood allows to separate red blood from antibodies, so the recipient won't receive the donors antibodies as well. (brainscape.com)
  • With the generous support of volunteer blood donors and the community, the Rhode Island Blood Center has developed an industry leading blood collection program. (ribc.org)
  • Figure 1: Amplification of large DNA fragments from mammalian blood prepared with the DNA Isolation Kit for Mammalian Blood. (sigmaaldrich.com)
  • The double dose buffy coat method requires fewer buffy coats and reduces the use of consumables by up to 50% (e.g. pooling sets, filter sets, platelet additive solution, and sterile connection wafers) compared to preparation and treatment of single dose buffy coat platelet units. (diva-portal.org)
  • Conventional storage of blood-derived fractions relies on cold. (imagene.eu)
  • Blood was separated into plasma and buffy coat fractions. (aacrjournals.org)