Transcription of Alu DNA elements in blood cells of sporadic Creutzfeldt-Jakob disease (sCJD). (1/25)
Alu DNA elements were long considered to be of no biological significance and thus have been only poorly defined. However, in the past Alu DNA elements with well-defined nucleotide sequences have been suspected to contribute to disease, but the role of Alu DNA element transcripts has rarely been investigated. For the first time, we determined in a real-time approach Alu DNA element transcription in buffy coat cells isolated from the blood of humans suffering from sporadic Creutzfeldt-Jakob disease (sCJD) and other neurodegenerative disorders. The reverse transcribed Alu transcripts were amplified and their cDNA sequences were aligned to genomic regions best fitted to database genomic Alu DNA element sequences deposited in the UCSC and NCBI data bases. Our cloned Alu RNA/cDNA sequences were widely distributed in the human genome and preferably belonged to the "young" Alu Y family. We also observed that some RNA/cDNA clones could be aligned to several chromosomes because of the same degree of identity and score to resident genomic Alu DNA elements. These elements, called paralogues, have purportedly been recently generated by retrotransposition. Along with cases of sCJD we also included cases of dementia and Alzheimer disease (AD). Each group revealed a divergent pattern of transcribed Alu elements. Chromosome 2 was the most preferred site in sCJD cases, besides chromosome 17; in AD cases chromosome 11 was overrepresented whereas chromosomes 2, 3 and 17 were preferred active Alu loci in controls. Chromosomes 2, 12 and 17 gave rise to Alu transcripts in dementia cases. The detection of putative Alu paralogues widely differed depending on the disease. A detailed data search revealed that some cloned Alu transcripts originated from RNA polymerase III transcription since the genomic sites of their Alu elements were found between genes. Other Alu DNA elements could be located close to or within coding regions of genes. In general, our observations suggest that identification and genomic localization of active Alu DNA elements could be further developed as a surrogate marker for differential gene expression in disease. A sufficient number of cases are necessary for statistical significance before Alu DNA elements can be considered useful to differentiate neurodegenerative diseases from controls. (+info)Methylprednisolone prevents tumour necrosis factor-alpha-dependent multinucleated giant cell formation. (2/25)
(+info)The Mirasol Pathogen Reduction Technology system and quality of platelets stored in platelet additive solution. (3/25)
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(+info)Merkel cell polyomavirus DNA sequences in the buffy coats of healthy blood donors. (7/25)
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Buffy coat
Marketing, Metric (2021-03-25). "What is Buffy Coat in Blood? Buffy Coat Preparation and Buffy Coat Cell Extraction". Akadeum ... The layer beneath the buffy coat contains granulocytes and red blood cells.[citation needed] The buffy coat is commonly used ... "A preliminary comparative report of quantitative buffy coat and modified quantitative buffy coat with peripheral blood smear in ... The buffy coat is the fraction of an anticoagulated blood sample that contains most of the white blood cells and platelets ...
African trypanosomiasis
For blood samples, these include centrifugation followed by examination of the buffy coat; mini anion-exchange/centrifugation; ... The first uses dried blood, while the other two use whole blood samples. A 2002 study found the wb-CATT to be the most ... and the quantitative buffy coat (QBC) technique. For other samples, such as spinal fluid, concentration techniques include ... Diagnosis is by finding the parasite in a blood smear or in the fluid of a lymph node. A lumbar puncture is often needed to ...
Surra
Using HCT trypanosomes can be detected in the blood even in field conditions. Buffy coat can be used to increase detection. ... such as wet blood film, and stained blood smears are used because so far, the best identifier is looking at the blood of the ... These blood sucking insects are Stomoxys, Haematobia (also known as Lyperosia), Haematopota, and Ornithordorus.[citation needed ... This means a good indicator of disease would be looking at trypanosome density in capillary blood and connective tissue. After ...
Peripheral blood mononuclear cell
These cells can be extracted from whole blood or buffy coat samples using a hydrophilic colloid and density gradient ... "Protocol for PBMC isolation from buffy coat samples". www.reprocell.com. Retrieved 2022-08-09. Bittersohl, Heike; Steimer, ... A peripheral blood mononuclear cell (PBMC) is any peripheral blood cell having a round nucleus. These cells consist of ... Peripheral blood cell Delves, Peter, et al. Roitt's Essential Immunology, 11th Ed. ISBN 978-1-4051-3603-7 The impact of food ...
Dirofilaria immitis
... separating the plasma from the red blood cells. These layers are divided by the buffy coat. The buffy coat consists of the ... The tube is snapped at the buffy coat and added to a slide for microscopic examination. Adding methylene blue stain to the ... Microfilarial detection is accomplished by the using one of the following methods: Direct blood smear A blood sample is ... The blood flowing back to the heart is blocked due to the large mass of worms. This is life-threatening and the only treatment ...
Platelet transfusion refractoriness
"Platelets, Pooled, Buffy Coat Derived, in Additive Solution and Plasma, Leucocyte Depleted". www.transfusionguidelines.org. ... Blood. 119 (23): 5553-5562. doi:10.1182/blood-2011-11-393165. ISSN 1528-0020. PMC 3369689. PMID 22496156. Petraszko, Tanya; ... Some blood banks maintain records of the estimated number of platelets in each unit. Current requirements in the US stipulate ... Canadian Blood Services. Estcourt, Lise J; Malouf, Reem; Hopewell, Sally; Trivella, Marialena; Doree, Carolyn; Stanworth, Simon ...
Blood donation
"In Vitro Evaluation of Buffy Coat Derived Platelet Concentrates in SSP+ Platelet Storage Medium". Transfusion Medicine. 16: 26 ... Blood bank#History (history of blood donation) Blood donation restrictions on men who have sex with men Blood substitute James ... Blood is something we all expect to be there for us when we need it, yet only 4% of us give blood. "Canadian Blood Services". ... "Facts About Blood". America's Blood Centers. Archived from the original on 2013-11-08. Retrieved 2013-01-04. "Give Blood". NHS ...
Trypanosomiasis
Motile organisms may be visible in the buffy coat when a blood sample is spun down. Serological testing is also common. The use ... Diagnosis relies on recognition of the flagellate on a blood smear. ... blood in urine, aching muscles and joints, headaches and irritability. In the first phase, the patient has only intermittent ...
Diagnosis of malaria
Quantitative buffy coat (QBC) is a laboratory test to detect infection with malaria or other blood parasites. The blood is ... In such cases the medical technologist may obtain a buffy coat, from which a blood smear is made. This smear contains a much ... The mainstay of malaria diagnosis has been the microscopic examination of blood, utilizing blood films. Although blood is the ... the fluorescing parasites can then be observed under ultraviolet light at the interface between red blood cells and buffy coat ...
Samuel George Shattock
... and the significance of the buffy coat in the shed blood". The Journal of Pathology and Bacteriology. 6 (3): 303-314. doi: ...
Alcelaphine gammaherpesvirus 2
... infections by nested amplification of viral DNA in bovine blood buffy coat specimens". Journal of Veterinary Diagnostic ...
Boom method
Starting material are for example, whole blood, blood serum, buffy coat, urine, feces, cerebrospinal fluid, sperm, saliva, ... The silica coated beads coated various magnetic particles (magnetic carrier) with silica are often used. Maghemite particle (γ- ... Today, the assay characterized by using silica coated magnetic beads seems to be the most common. Therefore, in this article, " ... How do SPRI beads work? DNA Extraction Methods For Large Blood Volumes B Vogelstein and D Gillespie;"Preparative and analytical ...
Chagas disease
... or its buffy coat, for motile parasites; or by preparation of thin and thick blood smears stained with Giemsa, for direct ... or by inoculating animals with the person's blood. In the blood culture method, the person's red blood cells are separated from ... Transfusion with the blood of an infected donor infects the recipient 10-25% of the time. To prevent this, blood donations are ... T. cruzi can survive in refrigerated stored blood, and can survive freezing and thawing, allowing it to persist in whole blood ...
Blood
... buffy coat (middle, thin white layer) and erythrocyte layer (bottom, red layer) can be seen. Blood circulation: Red = ... mixed-blood and blood relative. Autotransfusion Blood as food Blood pressure Blood substitutes ("artificial blood") Blood test ... and the blood cells it carries, peripheral blood cells. Blood is composed of blood cells suspended in blood plasma. Plasma, ... of blood is blood plasma, a fluid that is the blood's liquid medium, which by itself is straw-yellow in color. The blood plasma ...
Carcinocythemia
... a smear can be prepared from the buffy coat of the blood sample to concentrate the cells. Tumour cells in peripheral blood may ... The amount of tumour cells on the blood smear can range from 1 to 80 percent of the total white blood cell count, with lower ... could contribute to the presence of tumour cells in the blood. Carcinocythemia can be detected on a routine blood smear ... Tumour cells are often found at the edge of the blood smear due to their large size, so this area should be examined thoroughly ...
White blood cell
White cells are found in the buffy coat, a thin, typically white layer of nucleated cells between the sedimented red blood ... The complete blood cell count is a blood panel that includes the overall white blood cell count and differential count, a count ... All white blood cells have nuclei, which distinguishes them from the other blood cells, the anucleated red blood cells (RBCs) ... Histamine is responsible for widening blood vessels and increasing the flow of blood to injured tissue. It also makes blood ...
Fåhræus effect
He used the concept of buffy coat as the starting point of his work on red cell sedimentation and the more general problem of ... The Fåhræus effect is the decrease in average concentration of red blood cells in human blood as the diameter of the glass tube ... Considering steady laminar fully developed blood flow in a small tube with radius of r 0 {\displaystyle r_{0}} , whole blood ... c) The migration of blood cells from the tube wall to the axis depends on the particle size and not on the particle density. (d ...
Blood fractionation
... see Blood plasma fractionation), the buffy coat, which is a thin layer of leukocytes (white blood cells) mixed with platelets ... when centrifuged the silicone gel forms a layer on top of the buffy coat, allowing the blood serum to be removed more ... Blood fractionation is the process of fractionating whole blood, or separating it into its component parts. This is typically ... Blood plasma fractionation Blood Plasma Pooling (from the Bloodbook.com website) Statement by Dr. Kathryn Zoon, Food and Drug ...
Plateletpheresis
Platelet-rich plasma (PRP) is widely used in North America and buffy coat (BC) is more widely used in Europe. Platelets are the ... Since it takes up to 3 liters of whole blood (the amount of a dozen blood bags) to generate a dose of platelets, white blood ... Blood accounts for about 8% of body weight, so a 50 kg (110 lb) donor has about four liters of blood. No more than 50% of a ... Even though red blood cells can also be collected in the process, many blood donation organizations do not do so because it ...
HUNT Biobank
Blood sampling follows a strict quality protocol, collecting serum, plasma, buffy coat, immortalized cells for cell line ... collecting blood and urine samples and by clinical examination. In connection with the HUNT3 survey (2006-2008), HUNT Biobank ...
Fractionation
... after which three major blood components can be visualized: plasma, buffy coat and erythrocytes(blood cells). These separated ... The process of blood fractionation involves separation of blood into its main components. Blood fractionation refers generally ...
Babesia divergens
"The efficacy of the ultraviolet C pathogen inactivation system in the reduction of Babesia divergens in pooled buffy coat ... Zintl, A; Mulcahy, G; Skerrett, HE; Taylor, SM; Gray, JS (2003). "Babesia divergens, a Bovine Blood Parasite of Veterinary and ... To Invade the Human Red Blood Cell". Infection and Immunity. 73 (1): 649-651. doi:10.1128/IAI.73.1.649-651.2005. PMC 538995. ...
Apheresis
The end product in most cases is the classic sedimented blood sample with the RBCs at the bottom, the buffy coat of platelets ... To stop the blood from coagulating, anticoagulant is automatically mixed with the blood as it is pumped from the body into the ... Blood does not contact the device and during the separation, blood does not exit the kit. Reinfusion - At the end of the ... Blood taken from a healthy donor can be separated into its component parts during blood donation, where the needed component is ...
Phytohaemagglutinin
Prior to 1960, crude extracts of PHA were known to coat the surface of red blood cells, make them heavier, and thereby improve ... the separation of the white cell buffy coat. Peter Nowell, an immunologist and pathologist at the University of Pennsylvania in ... PHA-E cause erythrocytes (red blood cells) to clump. PHA-L causes leukocytes (white blood cells) to clump. Phytohaemagglutinin ... Stimulation of peripheral blood lymphocytes by phytohaemagglutinin presents a classic model of transition of cells from the ...
Ficoll
... a layer of mono-nuclear cells called buffy coat (PBMC/MNC), Ficoll-Paque, and erythrocytes & granulocytes which should be ... These products allow blood to be layered on much more quickly without mixing polysaccharide and blood. An example of such a ... This allows the blood sample to be rapidly pipetted onto the insert, avoiding the need for overlaying it directly onto Ficoll- ... Major blood clotting may sometimes occur in the PBMC layer. Ethylene diamine tetra-acetate (EDTA) and heparin are commonly used ...
Leishmaniasis
Buffy-coat preparations of peripheral blood or aspirates from marrow, spleen, lymph nodes, or skin lesions should be spread on ... The visceral form starts with skin ulcers and later presents with fever, low red blood cell count, and enlarged spleen and ... Additionally, visceral disease can be diagnosed by blood tests. Leishmaniasis can be partly prevented by sleeping under nets ... Amastigotes are seen within blood and spleen monocytes or, less commonly, in circulating neutrophils and in aspirated tissue ...
Blood bank
The layer between the red cells and the plasma is referred to as the buffy coat and is sometimes removed to make platelets for ... A blood bank is a center where blood gathered as a result of blood donation is stored and preserved for later use in blood ... Several types of blood transfusion exist:[citation needed] Whole blood, which is blood transfused without separation. Red blood ... "Circular of Information for the use of Human Blood and Blood Components" (PDF). AABB, ARC, America's Blood Centers. p. 16. ...
Photopheresis
... is a form of apheresis and photodynamic therapy in which blood is subject to apheresis to separate buffy coat (WBC + platelets ... Blood irradiation therapy ICD10 Procedure code: this was assigned based on cross-walking from the ICD9 code at ICD10DAta.com ... from whole blood, chemically treated with 8-methoxypsoralen (instilled into a collection bag or given per os in advance), ...
Whole blood
... buffy coat (used to make platelets), and red blood cells. The third method is sedimentation: the blood simply sits overnight ... Whole blood is made up of red blood cells, white blood cells, platelets, and blood plasma. It is best within a day of ... Historically, blood was transfused as whole blood without further processing. Most blood banks now split the whole blood into ... Whole blood (WB) is human blood from a standard blood donation. It is used in the treatment of massive bleeding, in exchange ...
Platelet
... "buffy coat" layer, which includes the platelets and the white blood cells. The "buffy coat" is isolated in a sterile bag, ... On a stained blood smear, platelets appear as dark purple spots, about 20% the diameter of red blood cells. The smear is used ... Although thrombosis, blood coagulation in intact blood vessels, is usually viewed as a pathological immune response, leading to ... Apheresis platelets are collected using a mechanical device that draws blood from the donor and centrifuges the collected blood ...
List of birds of Nepal
They are smallish birds with tails that are usually long and pointed, and tend to be drab brownish or buffy all over. Striated ... Gallus gallus Blood pheasant, Ithaginis cruentus Himalayan monal, Lophophorus impejanus Snow partridge, Lerwa lerwa Satyr ... and feathers that are excellent at shedding water due to an oily coating. Fulvous whistling-duck, Dendrocygna bicolor (A) ...
Buffy
... a color often used in description of birds Buffy (dog), Russian President Vladimir Putin's dog Buffy coat, a component of blood ... Look up Buffy in Wiktionary, the free dictionary. Buffy is a pet form of the female given name Elizabeth. Buffy or Buffie may ... known as Buffy Buffy (rapper) (born Kim Ju-hyeon, 1995) member of South Korean band MADTOWN Buffy Summers, the title character ... The Buffy EP, 1999 EP by Velvet Chain 2004 XR190, trans-Neptunian object, nicknamed Buffy Buffy the Vampire Slayer ( ...
Magic Mirror (Snow White)
... using the part-fairy blood of Danielle's son Jakub - Danielle having some fairy blood in her from her mother's side of the ... Before declaring Sidney free, the Snow Queen advises Sidney to get a warm coat since it is "going to get cooler around here." ... voiced by Buffy Sainte-Marie) is a shiny flat rock. It told Sly Fox that it was the fairest in the land until White Snow came ...
List of birds of Asia
They are smallish birds with tails that are usually long and pointed, and tend to be drab brownish or buffy all over. ... and feathers that are excellent at shedding water due to an oily coating. Spotted whistling-duck, Dendrocygna guttata Plumed ... Gallus varius Blood pheasant, Ithaginis cruentus Himalayan monal, Lophophorus impejanus Sclater's monal, Lophophorus sclateri ... Ketupa flavipes Buffy fish-owl, Ketupa ketupu Northern hawk owl, Surnia ulula Eurasian pygmy-owl, Glaucidium passerinum Asian ...
Maenad
The Buffy the Vampire Slayer novel Go Ask Malice: A Slayer's Diary depicts maenads as corrupted human beings in service of the ... Maenads are the adopted symbol of Tetovo in North Macedonia, depicted prominently of the city's coat of arms. The inclusion of ... Having symbolically eaten his body and drunk his blood, the celebrants became possessed by Dionysus. Maenads are found in later ... Charlaine Harris' The Southern Vampire Mysteries novels and its television adaptation, HBO's True Blood (2nd season, aired in ...
List of lakes of Ontario: B
Blowing Lake Blowout Lake Bloxham Lake Bludgeon Lake Blue Cedar Lake Blue Chalk Lake Blue Coat Lakes Blue Goose Lake Blue Hawk ... Blithfield Long Lake Blob Lake Bloch Lake Block Lake Block's Pond Blockville Lake Blonde Lake Blondin Lake Blood Lake Bloody ... Budworm Lake Buell Lake Buells Creek Reservoir Buff Lake Buffalo Island Lake Buffalo Lake Buffington Lake Bufflehead Lake Buffy ...
Spooks (series 1)
... best known for his work on Buffy the Vampire Slayer at the time, played Peter Salter. Despite his status as a former Buffy ... candy-coated escapism and romanticism of Alias, or the white-knuckled, thrill-a-minute intensity of 24. [Spooks] is its own ... but lost to Peter Bowker's work on television film Flesh and Blood. In the Craft awards, Julian Slater, Nigel Heath, Michael ...
Granulocyte transfusion
... is to combine the buffy coats (the fraction of blood containing white blood cells) from multiple routine blood donations to ... 297 The donor blood undergoes leukapheresis, a process by which white blood cells are separated from red blood cells and plasma ... During the blood collection, the donor's blood is mixed with hydroxyethyl starch, which helps separate the granulocytes from ... They are required to meet the general criteria for blood donation. Because the granulocyte concentrate also contains red blood ...
Three Coins in a Fuchsbau
A close-up shows Hitler wearing the coins on his coat, and Nick sees him transform into a Blutbad. The episode was viewed by ... I can think of many different shows that have used this different structure to brilliant effect - the Buffy bottle episode ' ... Blue Bloods, and Undercover Boss. This was a 10% increase in viewership from the previous episode, which was watched by 4.79 ...
Frankenstein in popular culture
the Creature from Blood Cove. In this film, Frankenstein's monster is resurrected to fight terrorists along with a half-fish, ... Buffy the Vampire Slayer has also faced "Frankensteinian" creations. In the season two episode "Some Assembly Required, the ... Two further novels were published in the series by Black Coat Press. The books, The Quest of Frankenstein and The Triumph of ...
List of birds of East Timor
They are smallish birds with tails that are usually long and pointed, and tend to be drab brownish or buffy all over. Javan ... Thick-billed flowerpecker, Dicaeum agile Red-chested flowerpecker, Dicaeum maugei Blood-breasted flowerpecker, Dicaeum ... and feathers that are excellent at shedding water due to an oily coating. Wandering whistling-duck, Dendrocygna arcuata Radjah ...
Spike (Buffy the Vampire Slayer)
She consoled him, drained him of blood and made him drink of her blood, thus transforming him into a vampire - "siring" him, in ... When the coat was destroyed by a bomb from the Immortal in Italy, Spike heartbrokenly declared it to be irreplaceable; but the ... Dracula". Buffy the Vampire Slayer. Season 5. Episode 1. The WB. "A Buffy Bestiary" Buffy the Vampire Slayer season 2 DVD ... making his first appearance the deadliest of any of Buffy's "Big Bads", as he very nearly kills Buffy, but Buffy's mother ...
Bolivia
Crabtree, J.; Buffy, G.; Pearce, J. (1988). "The Great Tin Crash: Bolivia and the World Tin Market". Bulletin of Latin American ... The symbols of the State are the red, yellow and green tri-color flag; the Bolivian national anthem; the coat of arms; the ... The Bolivia Reader: History, Culture, Politics (Duke University Press, 2018). Young, Kevin A. Blood of the earth: resource ...
Phodopus
No yellow or buffy highlights on the sides where the darker upper body fur meets the lighter underbody fur. [Ears small.] → ... The fur on the abdomen is grey, and the coat colour turns grey during moulting. Phodopus species display a variety of ... Feoktistova, N. Yu.; Kropotkina, M. V.; Naidenko, S. V. (2010). "Seasonal Changes of Steroid Levels in Blood Plasma of Three ... May have yellow or buffy highlights on the sides where the darker upper body fur meets the lighter under body fur. [Ears larger ...
List of Madagascar (franchise) characters
... which backfired in its first use because he forgot to take the warm-blooded/cold-blooded conflict into account when switching ... Muffy, Buffy and Fluffy (voiced by Kristen Schaal) are three adorable, cute, and cuddly rabbits who reside in the Central Park ... He is bald and wears a white lab coat. Skipper often voices dislike of him because of his use of needles and when injured, he ... It is revealed at the end of the contest that he only wants to make Mort turn to evil, as is in his blood, and help him fulfill ...
Tanya Tagaq
In 2017, Tagaq and fellow Polaris laureate Buffy Sainte-Marie collaborated on the single "You Got to Run (Spirit of the Wind ... Her 2008 album Auk/Blood (ᐊᐅᒃ Inuktitut syllabics) features collaborations with Mike Patton, among others. In 2011, she ... Tagaq reported that she had been banned from Facebook for posting a photo of a sealskin coat. The Aboriginal Peoples Television ... Martineau, Jarrett (February 22, 2017). "Queens of Indigenous Music Buffy Ste-Marie and Tanya Tagaq Unite for "You Got To Run ( ...
Grizzly Tales for Gruesome Kids (book)
Because she can not find her old clothes, she improvised with bath towels, a fishing hat, wellington boots and a fur coat, and ... A teacher had inspired him to write whatever he wanted, so he wrote a story that featured blood and mutilation and read it ... The army ambush her with nets and sticks, and surround her tightly as Colonel Buffy announces they had come to arrest her. ... And there was so much blood!" After Royds' suggestion, he realised as he wrote more stories that "what I was writing was a ...
List of birds of Bhutan
They are smallish birds with tails that are usually long and pointed, and tend to be drab brownish or buffy all over. Striated ... Gallus gallus Blood pheasant, Ithaginis cruentus Himalayan monal, Lophophorus impejanus Snow partridge, Lerwa lerwa Satyr ... and feathers that are excellent at shedding water due to an oily coating. Fulvous whistling-duck, Dendrocygna bicolor (A) ...
Fright Night
"I said to Morry, 'Thank you, no, I'll just use a coat hanger!'" Co-star Ragsdale recalled one instance when Sarandon spent an ... The script, penned by Buffy the Vampire Slayer alumna Marti Noxon, loosely followed the plot of the original film. Charley ( ... New Blood in 2013. In October 2020, Tom Holland confirmed that he was writing a direct sequel to the original film titled ... after draining blood from his victims. Stark and Sarandon had not picked up on Holland's intended gay subtext when they were ...
Joseph Lister
... the nature of the buffy coat; with a concise medical view of the state of the blood in disease; and an account of the powers of ... He believed that pigment reflected the activity of blood vessels, though it was the slowing of blood flow that initiates the ... Thirdly, some of the blood in the most injured blood-vessels slowed down in its flow and coagulated. Redness occurred which, ... This refuted Wharton's conclusions, in his paper Observations on the State of the Blood and the Blood-Vessels in Inflammation. ...
Doppelgänger
Clive Barker's story "Human Remains" in his Books of Blood is a doppelgänger tale, and the doppelgänger motif is a staple of ... In Buffy the Vampire Slayer's season three episode "Doppelgangland" (originally aired Feb. 23, 1999), Willow encounters her ... without a coat or jacket - he passed again - now as he passed both times the same way - and as from the side towards which he ...
Queen Elizabeth The Queen Mother
Elizabeth's coat of arms was the royal coat of arms of the United Kingdom (in either the English or the Scottish version) ... On 1 August 2001, Elizabeth had a blood transfusion for anaemia after suffering from mild heat exhaustion, though she was well ... My Darling Buffy: The Early Life of The Queen Mother, Headline Book Publishing, ISBN 978-0-7472-7387-5 Hogg, James; Mortimer, ...
Genomics|Resources|Books|HuGE|Part 2, Chapter 5 Tables
Whole blood. (anticoagulated or blood clots). Buffy coat. 100 - 400 μg/10 mLc. ~200 μg/mLc. - relatively low-cost storage (-80 ... Blood Spots. 12 - 42 ng/μL (adults)a. 43 - 78 ng/μL (neonates)a. 1/4 inch punch from 75 μL volume yields about 12 μL of bloodb ... with current technology as little as 2.5 ng per SNP so that scores to hundreds of genotypes could be obtained from one blood ...
Clonal architecture in mesothelioma is prognostic and shapes the tumour microenvironment | Nature Communications
Isolation of plasma and buffy coat from whole blood. Blood samples were collected in EDTA tubes and blood components (buffy ... were isolated by carefully pipetting the buffy coat layer and transferred to 1.5 mL tubes. Both plasma and buffy coat aliquots ... The extraction of germline DNA was performed using 200 µl of buffy coat and was carried out using the QiAamp DNA blood mini kit ... Prior to each EPD, blood was obtained in five 7.5-ml blood tubes containing EDTA. One of these blood tubes was frozen whole. ...
Maxwell® RSC Buffy Coat DNA Kit
... automated method for extraction of genomic DNA from 50-250µl of buffy coat samples using the Maxwell RSC Instrument. ... The Maxwell RSC Buffy Coat DNA Kit is a simple, ... Maxwell® RSC Whole Blood DNA Kit. Extracts DNA from whole blood ... The Maxwell® RSC Buffy Coat DNA Kit is a simple, automated method for extraction of genomic DNA from 50-250µl of buffy coat ... Automated DNA Purification from Buffy Coat Samples. *Purifies high yields of DNA from 50-250µl of buffy coat samples in about ...
Effects of platelet clumping on platelet concentrations measured by use of impedance or buffy coat analysis in dogs in: Journal...
... using impedance and quantitative buffy coat analyzers. Results-Platelet clumps were evident in all 100 blood smears, but the ... Using the buffy coat analyzer, some ADPtreated samples had increased PC; however, significant differences were not detected ... In contrast, the PC detected by use of the buffy coat analyzer were sometimes increased. Useful information can be obtained by ... Sample Population-50 blood samples obtained from 30 dogs. Procedure-10 blood samples containing platelet clumps were used and ...
Cutaneous T-Cell Lymphoma: Practice Essentials, Background, Pathophysiology
Complete blood count with differential; review the buffy coat smear for Sézary cells ... Prognostic significance of tumor burden in the blood of patients with erythrodermic primary cutaneous T-cell lymphoma. Blood. ... circulating in the peripheral blood or other evidence of a significant malignant T-cell clone in the blood, such as clonal T- ... Blood eosinophilia at baseline may also serve as a prognostic factor in patients with primary cutaneous T-cell lymphoma. [47] ...
The IoPPN Genomics & Biomarker Core Facility
... or constituent cell types of white blood cells (buffy coat) and red blood cells. These separate components then are used for ... easyBlood is able to detect plasma, serum, buffy coat and gel separator fractions in centrifuged blood tubes and automatically ... The system uses CCD camera technology, software and precision hardware to identify the three fractions of blood and calculate ... Sample preparation often requires the splitting of blood samples into its basic components such as serum or plasma, ...
Biology | Free Full-Text | The Platelet Fraction Is a Novel Reservoir to Detect Lyme Borrelia in Blood
The low bacterial burden in vasculature and lack of consensus around blood-based isolation of the causative pathogen, Borrelia ... Despite the widespread reliance on serum and plasma as analytes, we found that the platelet fraction of blood concentrates ... We therefore addressed methodological optimization of Borrelia recovery from blood, first by analyzing existing protocols, and ... To enhance pathogen viability and cultivability during whole blood collection, citrate anticoagulant is superior to more ...
Bacterial Sepsis Guidelines: Guidelines Summary
Positive buffy coat smear result OR several positive blood culture results with a pathogenic organism ... Detection of bacteremia by buffy coat smears. Scand J Infect Dis. 1987. 19(2):215-7. [QxMD MEDLINE Link]. ... blood products, anticoagulants, immunoglobulins, mechanical ventilation, sedation, analgesia, glucose control, blood ... 3. For adults suspected of having sepsis, we suggest measuring blood lactate.. Weak; low quality of evidence. ...
Internet Scientific Publications
buffy coat was carefully aspirated with sterile pipette. enough Phosphate Buffer Saline (PBS) was added to the PBMCs to make ... Blood was obtained in a sterile heparinized tube. 1:1 dilution of blood with PBS was made. 5ml was overlaid carefully over 2.5 ... The LD50 values of all the plant extracts against Peripheral blood mononuclear cells (PBMC) were determined by MTT assay (Fig 1 ... Isolation of Peripheral blood mononuclear cells (PBMC). (Hudson and Hay, 1989) ...
Complete Kit for Human Whole Blood CD34+ Cell Isolation | STEMCELL Technologies
Isolate CD34+ cells from human whole blood or buffy coat with this simple two-step method. ... Whole Blood CD34+ Cells is a simple two-step method that is designed to isolate CD34+ cells from whole blood or buffy coat.. ... Figure 1.Typical CD34 Selection Profile on Human Whole Blood Starting with whole peripheral blood, the CD34+ cell content of ... Complete Kit for Human Whole Blood CD34+ Cells (Catalog #15086). *RosetteSep™ Human Hematopoietic Progenitor Cell Enrichment ...
QIAamp DNA Blood Kits
... mitochondrial or viral DNA from blood and other body fluids ... buffy coat, plasma, serum, bone marrow, lymphocytes and body ... QIAamp DNA Blood Mini Kit. QIAamp DNA Blood Midi Kit. QIAamp DNA Blood Maxi Kit. QIAamp DNA Blood Maxi Kit. ... Whole blood, body fluids. Whole blood, body fluids. Whole blood, body fluids. Whole blood, body fluids. ... QIAamp DNA Blood Kits. QIAamp DNA Blood Kits. For purification of genomic, mitochondrial or viral DNA from blood and other body ...
WHO EMRO | Effect of methoxyverapamil, diltiazem, morphine and their combination on the formation of irreversibly sickled cells...
The heparinized venous blood was centrifuged at 1500 g (3000 rpm) for 5 minutes. Plasma and buffy coat were removed by ... From each patient, 5 ml of venous blood was collected in heparinized tubes. Experiments were performed immediately after blood ... Mechanical properties of oxygenated red blood cells in sickle-cell (HbSS) disease. Blood, 1984, 63(1):73-82. ... Cytosolic free calcium levels in sickle red blood cells. Blood, 1987, 69:1469-74. ...
FACS Sorting Shared Facility (FSSF) - Erasmus MC
MultiMACS™ X | Fully automated cell isolation system | Instruments | Singapore
Whole blood. - Buffy coat. - Leukapheresis sample. - LRSC. - Bone marrow. - Single-cell suspensions from dissociated tissue or ... Whole Blood, and Buffy Coat) with the StraightFrom® MicroBead portfolio, untouched isolation from PBMCs with MACS Isolation ... Isolation of functional cells straight from buffy coat, LRSC, and Leukopak®. *Workflow automation and parallelization improves ... LD Columns are optimized for depletion and Whole Blood Columns for cell isolation directly from whole blood, bone marrow, and ...
Quantitative recovery of proviral HIV-1 DNA from leukocytes by the Dried Buffy Coat Spot method for real-time PCR determination...
keywords = "DBCS (Dried Buffy Coat Spot), DBS (Dried Blood Spot), HIV-1 (human immunodeficiency virus type 1), HIV-1 pol, Human ... buffy coat). This study describes a new portable Dried Buffy Coat Spot (DBCS) assay able to provide a quantitative proviral HIV ... buffy coat). This study describes a new portable Dried Buffy Coat Spot (DBCS) assay able to provide a quantitative proviral HIV ... buffy coat). This study describes a new portable Dried Buffy Coat Spot (DBCS) assay able to provide a quantitative proviral HIV ...
Plasma Antibodies against Trichomonas vaginalis and Subsequent Risk of Prostate Cancer | Cancer Epidemiology, Biomarkers &...
Upon arrival, specimens were centrifuged; aliquoted into plasma, buffy coat, and erythrocytes; and stored in liquid nitrogen. ... PSA test after blood draw (%)** 99.6 99.7 1.00 100.0 99.4 0.52 Digital rectal examination after blood draw (%)** 98.1 96.8 0.16 ... PSA test after blood draw (%)** 99.6 99.7 1.00 100.0 99.4 0.52 Digital rectal examination after blood draw (%)** 98.1 96.8 0.16 ... These participants were similar to those who did not provide a blood sample on demographic and dietary factors. Blood samples ...
Evidence for Seeding of β-Amyloid by Intracerebral Infusion of Alzheimer Brain Extracts in β-Amyloid Precursor Protein...
1994) Blood buffy coat from Alzheimers disease patients and their relatives does not transmit spongiform encephalopathy to ... 1991) Search for transmissible agent in Alzheimers disease: studies of human buffy coat. Curr Top Microbiol Immunol 172:275- ... Thearrowheads denote the same blood vessel that has been transversely sectioned in a and b. Scale bar, 100 μm. ... 5b), and numerous blood vessels were affected, especially along the hippocampal fissure (Figs. 3, 4). A small number of plaques ...
A tandem CD19/CD20 CAR lentiviral vector drives on-target and off-target antigen modulation in leukemia cell lines | Journal...
The buffy coat fractions of the donated blood would be discarded if unclaimed. All blood collections were de-identified, and ... Ethics approval and consent to participate Human T cells were extracted from buffy coat fractions of blood donations collected ... Processed buffy coats were purchased from OBI (Oklahoma City, OK). The CD4-positive and CD8-positive human T cells were ... Whole blood was collected from healthy volunteers at Oklahoma Blood Institute (OBI) with donors written consent. ...
US Patent for Multi-part differential analyzing apparatus utilizing light scatter techniques Patent (Patent # 5,125,737 issued...
In this mode, there is provided a purified white cell sample, for example, by centrifugation, density, or buffy coating ... A method for differentiating at least one white blood cell subpopulation in a blood sample, whereby differentiation is based ... A method for differentiating at least one white blood cell subpopulation in a blood sample, whereby differentiation is based ... A method for differentiating at least one white blood cell subpopulation in a blood sample, whereby differentiation is based ...
Concordance of Genomic Alterations between Circulating Tumor DNA and Matched Tumor Tissue in Chinese Patients with Breast Cancer
i,Purpose,/i,. Circulating tumor DNA (ctDNA) served as a noninvasive method with less side effects using peripheral blood. ... Whole-blood samples were processed within three days after blood collection, and they were separated into plasma and buffy coat ... were extracted from tumor tissue and buffy coat using a Qiamp FFPE tissue kit (Qiagen, 56404, Germany) and TiANamp Blood DNA ... gDNAs from tumor tissue and buffy coat were sheared into 150-250 bp fragments by Covaris S220 (Covaris, S220, USA). The cfDNA ...
Babesiosis - Symptoms, diagnosis and treatment | BMJ Best Practice US
Babesiosis is transmitted to humans through the blood meal of ticks, the same vector as for Lyme disease and human granulocytic ... polymerase chain reaction and/or immunofluorescence assay or buffy coat smear for human granulocytic anaplasmosis ... Babesiosis is transmitted to humans through the blood meal of Ixodes ticks, the same vector as for Lyme disease and human ... Diagnosis is made by visualization of parasites on a peripheral blood smear or by molecular testing. ...
Equine Archives - Page 2 of 10 - Texas A&M Veterinary Medical Diagnostic Laboratory
... by microscopic evaluation of the buffy coat.. Specimen: 1 mL EDTA whole blood ... Buffy Coat Examination. Detection of infectious agents (i.e. Hepatozoon spp., bacteria, spirochetes) and neoplastic cells (i.e ... Microscopic blood smear review for WBC differential, cellular morphology, and hemoparasites.. Specimen: 1 mL EDTA whole blood ... For birds, 0.3 mL EDTA whole blood is adequate.. Blood, serum, and urine samples are tested on Mondays, Wednesdays, and ...
Comparative Analysis of T Cell Imaging with Human Nuclear Reporter Genes | Journal of Nuclear Medicine
Extraction laboratory workflow | LGC Biosearch Technologies
Withania somnifera modulates cancer cachexia associated inflammatory cytokines and cell death in leukaemic THP-1 cells and...
... antioxidants and apoptosis in leukaemic THP-1 cells and peripheral blood mononuclear cells (PBMCs). Cytotoxcity of WRE was ... Buffy coats containing PBMCs were obtained from the South African National Blood Service (2011/09). PBMCs were extracted by ... Buffy coats (5 ml) were layered onto equivolume histopaque-1077 (5 ml) in 15 ml polypropylene tubes and centrifuged (400×g, 21 ... ELISA plates were coated with capture antibody overnight (100 μl/well, 4 °C). Thereafter, plates were washed (3×) with wash ...
A preliminary analysis of a randomized study of buffy coat transfusions in renal transplantation<...
To better understand the effect of dose of blood and viability of white blood cells on anti-HLA antibody production and graft ... A preliminary analysis of a randomized study of buffy coat transfusions in renal transplantation. / Norman, D. J.; Barry, J. M. ... Norman, D. J., Barry, J. M., Durr, M., & Wetzsteon, P. (1985). A preliminary analysis of a randomized study of buffy coat ... Norman DJ, Barry JM, Durr M, Wetzsteon P. A preliminary analysis of a randomized study of buffy coat transfusions in renal ...
SerumSmearPlasmaMononuclear cellsPolymerase chain rePlateletsIsolationSalivaTransfusionCellsQuantitative buffyAssayDifferentialEDTABoneQuantificationConcentrationsUrea nitrogenPatient's bloodLymphocytesCord bloodPlateletPlacentaDonorsSamplesDonorHaemolysisLiverPurificationLeukocytesAcuteSmearsAutologousTransfusionsBorreliaCentersWhiteHumanDetectionFractionsTubesCellDilutionSampleUrinalysisLayerAnalysisGenesSpikeResistParasitesHospitals
Serum6
- Sample preparation often requires the splitting of blood samples into its basic components such as serum or plasma, or constituent cell types of white blood cells (buffy coat) and red blood cells. (kcl.ac.uk)
- easyBlood is able to detect plasma, serum, buffy coat and gel separator fractions in centrifuged blood tubes and automatically separates and aliquots these fractions into target containers or downstream applications. (kcl.ac.uk)
- QIAamp DNA Blood Kits provide silica-membrane-based DNA purification from whole blood, plasma, serum and other body fluids. (qiagen.com)
- A convenient 96-well format using centrifugation enables purification of DNA for labs that need high-throughput DNA purification from blood, buffy coat, plasma, serum, bone marrow, lymphocytes and body fluids. (qiagen.com)
- Blood, serum, and urine samples are tested on Mondays, Wednesdays, and Thursdays with a turnaround time of 5 days. (tamu.edu)
- Buffy coat is a thin layer, neutral or buff in color, formed between the blood and the platelet serum and are essentially plasma with suspended platelets. (drprpusa.com)
Smear8
- Diagnosis is made by visualization of parasites on a peripheral blood smear or by molecular testing. (bmj.com)
- Additionally, various technological advancements, such as the development of the Quantitative Buffy Coat (QBC) method and peripheral blood smear technique for diagnosing malaria, are acting as other growth-inducing factors. (imarcgroup.com)
- Further information about the type of anaemia present cannot be determined without looking a blood smear (which will be covered in the next session). (vin.com)
- Examination of a blood smear is necessary to determine whether an anaemia is regenerative or non-regenerative and we will cover that in the next session. (vin.com)
- Peripheral blood smear is typically the first step in diagnosis. (medscape.com)
- A thin smear or thick drop of blood is deposited on a microscope slide, which is then stained with either Wright or Giemsa, and examined under oil immersion. (medscape.com)
- Peripheral blood smear in relapsing fever. (medscape.com)
- Inexperience in reading smears or a low index of suspicion for the infection may also result in false-negative blood smear results. (medscape.com)
Plasma15
- Plasma and buffy coat were rocking for 90 minutes at room separated at each hospital, kept in liquid temperature [6] . (who.int)
- Blood and serotype identification were also specimens were obtained from patients for performed in 584 acute phase plasma by the diagnostic purpose. (who.int)
- Adequate federal regulations, recommendations, and guidelines for blood and plasma are already established and are not addressed in this document. (cdc.gov)
- Whole blood fractionation: centrifuge and separate the whole blood into plasma, buffy coat (white blood cells & platelets), and erythrocyte fractions. (abnova.com)
- For the automated processing of whole blood into separate components of plasma, buffy layer or red blood cells for downstream analysis or biobanking. (perkinelmer.com)
- It provides fully automated processing of up to 96 blood tubes per run, calculates and aspirates user specified amounts of plasma and/or buffy coat and can reformat into any consumable for downstream analysis (such as gDNA or cfDNA) or for long term biobanking storage. (perkinelmer.com)
- The JANUS ® G3 Blood iQ ™ Workstation fully automates the process of separating centrifuged whole blood into components of plasma, buffy coat and red blood cells. (perkinelmer.com)
- Collect the mononuclear cells (MCs) by pipetting the buffy coat (cell layer between gel barrier and plasma) into a sterile 15 ml conical centrifuge tube. (stembook.org)
- When separated the right way, the buffy coat will be mostly plasma and white blood cells (leukocytes) with 5X-7X platelets concentration with less than 10% RBC. (drprpusa.com)
- Yes, there is a third type of Platelet-Rich Plasma kits and those are buffy-coat based and that can do double-spin without introducing further Red Blood Cells into the mix. (drprpusa.com)
- 3.55 × 10 6 , 3.55 × 10 5 , or 3.55 × 10 4 EID 50 of H1N1 virus in 10 μl of PBS diluted as stated in Materials and Methods was mixed with 130 μl of PBS, plasma, or buffy coats for different periods of time indicated. (biomedcentral.com)
- B ). Comparison of RNA recovery from 10 μl of 3.55 × 10 6 EID 50 of H1N1 virus stored in PBS, plasma, or buffy coats. (biomedcentral.com)
- Before snapping the microhaematocrit tube to determine total solids (TS), look at the colour of the plasma above the red blood cells. (vin.com)
- Cloudy plasma suggests lipaemia and it may occasionally be pink in colour (looking like strawberry milk) because RBC fragility is increased in lipemic blood and so intravascular haemolysis may be seen. (vin.com)
- The fraction of a blood sample, following CENTRIFUGATION , that is distinguished as a thin light-colored layer between the RED BLOOD CELLS , underneath it, and the PLASMA , above it. (bvsalud.org)
Mononuclear cells3
- The antimicrobial potentials were observed with the extracts of Adathoda vasika, Ficus bengalensis and Solanum torvum The LD 50 value of all the plant extracts were determined against Peripheral blood mononuclear cells (PBMC) and the concentration below LD 50 was taken for further study to determine the antioxidant property. (ispub.com)
- We investigated the potential of an aqueous extract of the root of W. somnifera (W RE ) to modulate cytokines, antioxidants and apoptosis in leukaemic THP-1 cells and peripheral blood mononuclear cells (PBMC's). (biomedcentral.com)
- Here we demonstrate a protocol for the generation of human iPSCs from peripheral blood mononuclear cells (PBMCs) using a single floxedexcisable lentiviral vector constitutively expressing the 4 factors. (stembook.org)
Polymerase chain re2
- Diagnosis is made by detection of the virus by buffy-coat blood culture or by polymerase chain reaction (PCR) analysis. (scirp.org)
- To carry out polymorphism study, buffy coat of EDTA blood was used for DNA isolation followed by polymerase chain reaction and restriction fragment length polymorphism. (researchsquare.com)
Platelets4
- That's called "bloody PRP" and it is the least effective containing maybe 1-1.5x platelets if you're lucky - and a whole lot of Red and White Blood Cells. (drprpusa.com)
- It works because the gel has the density that's in between blood and platelets. (drprpusa.com)
- The kits that produce a visible buffy coat layer after centrifugation are the best at giving concentrations of platelets anywhere from 5X to 7X. (drprpusa.com)
- It is composed mostly of WHITE BLOOD CELLS and PLATELETS . (bvsalud.org)
Isolation2
- Whether you have many samples to process in parallel or large-volume blood samples, parallel cell isolation with a 24-well magnet ensures each sample is processed identically and in a reproducible manner. (miltenyibiotec.com)
- Virus isolation was accomplished by patients were hospitalized in six provincial inoculating buffy coat separated from 238 hospitals in Thailand: Charoenkrung Pracharak acute-phase specimens on C6/36 cell culture. (who.int)
Saliva1
- Blood, buffy coat, and saliva samples are typically extracted using our Kleargen e ™ chemistry . (biosearchtech.com)
Transfusion3
- According to the International Society for Blood Transfusion (ISBT), there are currently 308 red blood cell antigens classified into 30 blood group systems. (scielo.br)
- The department staff also participates in peripheral blood stem cell (PBSC) collection and provides special transfusion support to bone marrow transplant patients. (tmckolkata.com)
- BacT/ALERT system was introduced as one of the most sensitive, specific, and rapid screening test, which could be implemented in Blood Transfusion Centers. (walshmedicalmedia.com)
Cells15
- The Complete Kit for Human Whole Blood CD34+ Cells is a simple two-step method that is designed to isolate CD34+ cells from whole blood or buffy coat. (stemcell.com)
- RosetteSep™ binds unwanted cells to red blood cells (RBCs), forming immunorosettes, which sediment during density gradient centrifugation. (stemcell.com)
- Among sickle cells, irreversible sickle cells (ISCs), which maintain abnormally deformed shape even after prolonged oxygenation, have a three to seven times higher calcium level compared to normal red blood cells (RBCs) [1,2] and twice that of reversible sickle cells [3,4]. (who.int)
- bacteria, spirochetes) and neoplastic cells (i.e. mast cells) by microscopic evaluation of the buffy coat. (tamu.edu)
- conducted on blood samples from the It showed enhanced myelo-, erythro-, mother showed positive results (IgG and thrombopoesis with slight lym- antibody titer 1:128 against pro- phopenia but no leukemic cells. (cdc.gov)
- To better understand the effect of dose of blood and viability of white blood cells on anti-HLA antibody production and graft survival, a randomized study was undertaken. (elsevier.com)
- Starting from fresh or frozen blood, buffy coat, and soon tissue or cells, the new TruTip kits extract superior quality gDNA simultaneously from eight to 96 samples in 57 minutes, Akonni said. (genomeweb.com)
- Occasionally, viral inclusion bodies can be found in circulating leukocytes (white blood cells) in the beginning stages of the disease. (petplace.com)
- In one study, a Tet inducible version of the STEMCCA vector was employed9, which required the blood cells to be simultaneously infected with a constitutively active lentivirus encoding the reverse tetracycline transactivator. (stembook.org)
- In contrast to fibroblasts, peripheral blood cells can be collected via minimally invasive procedures, greatly reducing the discomfort and distress of the patient. (stembook.org)
- A simple and effective protocol for reprogramming blood cells using a constitutive single excisable vector may accelerate the application of iPSC technology by making it accessible to a broader research community. (stembook.org)
- Furthermore, reprogramming of peripheral blood cells allows for the generation of iPSCs from individuals in which skin biopsies should be avoided ( i.e. , aberrant scarring) or due to pre-existing disease conditions preventing access to punch biopsies. (stembook.org)
- These professional kits allow you choose to allow or disallow red and white blood cells in your final product depending on applications. (drprpusa.com)
- One positive aspect is that the gel also separates white blood cells. (drprpusa.com)
- You can get a completely amber product or red product depending on whether you want a little red blood cells in your end product or not. (drprpusa.com)
Quantitative buffy5
- The PC for each ADP-treated and untreated sample were measured, using impedance and quantitative buffy coat analyzers. (avma.org)
- IMSEAR at SEARO: Quantitative buffy coat: a special adjunct for diagnosis of malaria. (who.int)
- Mirdha BR, Samantray JC, Burman D, Mishra B, Ghimire P. Quantitative buffy coat: a special adjunct for diagnosis of malaria. (who.int)
- Quantitative buffy coats (QBC) technique was compared with conventional blood film technique for the diagnosis of malaria in a tertiary care hospital. (who.int)
- Quantitative buffy coat is 100 times more sensitive than thick films in vitro and may be useful in diagnosis of relapsing fever. (medscape.com)
Assay2
- This study describes a new portable Dried Buffy Coat Spot (DBCS) assay able to provide a quantitative proviral HIV-1 DNA recovery from the buffy coat. (elsevier.com)
- Assay sensitivity and specificity were evaluated against the whole blood dried blood spot (DBS) method. (elsevier.com)
Differential2
- The present invention relates generally to particle analyzing apparatus and more particularly to apparatus and method for achieving selective, discriminate, differential classification of individual blood cell types, for example, five basic types of leukocytes, by means of technology of the Coulter type without the utilization of cytochemical staining techniques or materials. (justia.com)
- Material and Methods: In this study we used the whole blood from 24 healthy human subjects with inform consent, The PCs were collected from these whole blood samples by differential Centrifugation and Buffy coat system. (walshmedicalmedia.com)
EDTA5
- Blood from each of 20 healthy dogs was divided between 2 EDTA tubes before and after platelet clumping was induced by adenosine diphosphate (ADP). (avma.org)
- We demonstrate that the container most commonly used to collect blood (EDTA) decreases Borrelia's ability to grow, and we identify a superior alternative (citrate). (mdpi.com)
- 1). Blood specimens were drawn into tubes with ethylene diamine tetaacetate (EDTA) anticoagulant and centrifuged. (who.int)
- For birds, 0.3 mL EDTA whole blood is adequate. (tamu.edu)
- Human whole blood: collect at least 4ml whole blood in K2-EDTA tube (purple cap) at room temperature. (abnova.com)
Bone4
- DNA from 17 chimpanzees (lung, spleen, or liver), form a separate novel genus, with the proposed name of 30 red colobus monkeys (buffy coat, blood, bone marrow, Partetravirus, within the subfamily Parvovirinae . (cdc.gov)
- AIM: To evaluate allele polymorphisms of the Rh, Kell, Duffy and Kidd blood group systems in a population of the State of Paraná METHODS: Rh, Kell, Duffy and Kidd blood group polymorphisms were evaluated in 400 unrelated blood or bone marrow donors from the northwestern region of Paraná State between September 2008 and October 2009. (scielo.br)
- Rh, Kell, Duffy and Kidd blood group polymorphisms were evaluated in 400 unrelated blood or bone marrow donors from the northwestern region of Paraná State between September 2008 and October 2009. (scielo.br)
- The department has a blood irradiator (BRIT) to modify the blood components and render them suitable for cancer and bone marrow transplant patients (immuno-compromised). (tmckolkata.com)
Quantification1
- DNA extraction: extract the genomic DNA from the buffy coat fraction followed by DNA purification and quantification. (abnova.com)
Concentrations3
- Objective -To determine whether platelet clumps are homogeneously distributed in blood samples, and whether platelet concentrations (PC) obtained by use of impedance and buffy coat analysis can be considered minimum values when platelet clumps are present. (avma.org)
- These drugs were tested in pharmacological concentrations and in higher concentrations on blood obtained from patients with sickle-cell anaemia. (who.int)
- A ). Comparison of RNA recovery from different concentrations of virus stored in buffy coats. (biomedcentral.com)
Urea nitrogen1
- Measurement of blood urea nitrogen by automated analyzer. (tamu.edu)
Patient's blood2
- To diagnose Lyme disease, a patient's blood is tested for antibodies that develop as part of the immune response. (mdpi.com)
- In the research setting, the patient's blood can be inoculated into mice and the spirochetes amplified in murine blood. (medscape.com)
Lymphocytes1
- On the complete blood count (CBC), a lymphopenia (decreased numbers of lymphocytes) may be seen secondary to the virus. (petplace.com)
Cord blood5
- To develop a better understanding of the link between tissue specificity and neonatal EWAS, and the contributions of genotype and prenatal factors, we compared genome-wide DNA methylation of cord tissue and cord blood, two of the most accessible surrogate tissues at birth. (biomedcentral.com)
- Sites of inter-individual variability in DNA methylation were mapped and compared across the two surrogate tissues at birth, i.e., cord tissue and cord blood. (biomedcentral.com)
- In comparison to cord blood, cord tissue showed higher inter-individual variability in the epigenome, with a lower proportion of CpGs influenced by genotype. (biomedcentral.com)
- 6 ] compared the DNA methylation status of seven candidate gene loci and repeat sequences ( LINE -1 and ALUYb7) in the genome across three infant tissues (cord blood, placenta, and early infancy buccal epithelium), and reported tissue-specific differences in DNA methylation levels for most of the tested loci. (biomedcentral.com)
- 8 ] found associations between intrauterine cigarette smoke exposure and aryl hydrocarbon receptor repressor DNA methylation in both cord blood and infant blood at 18 months, but not in placenta and buccal epithelium at birth. (biomedcentral.com)
Platelet5
- Results -Platelet clumps were evident in all 100 blood smears, but the amount of clumping varied considerably within some samples. (avma.org)
- Conclusions and Clinical Relevance -Platelet clumping was not homogeneous within blood samples. (avma.org)
- When platelet clumps were identified by direct examination of blood smears, the PC detected by use of the impedance analyzer could be considered minimum values. (avma.org)
- The Red Cross must collect more than 13,000 blood and platelet donations every day for the patients at about 2,600 hospitals nationwide, including 80 hospitals in Indiana. (redcrossblood.org)
- Blood and platelet donors can save time at their next donation by using RapidPass ® to complete their pre-donation reading and health history questionnaire online, on the day of their donation, from a computer or mobile device. (redcrossblood.org)
Placenta1
- El estudio de su placenta reveló una villitis crónica por CMV, en la que se observa una reacción granulomatosa, en lugar de la típica inflamación de células plasmáticas. (bvsalud.org)
Donors4
- Exclusion of prospective blood donors based on their acknowledged risk behaviors for human immunodeficiency virus (HIV) infection began in 1983 (1). (cdc.gov)
- In 1985, when tests for HIV antibody became available, screening prospective donors of blood, organs, and other tissues also began (2,3). (cdc.gov)
- We take for granted that blood will be there at the hospital when we need it, but it can only come from generous volunteer donors. (redcrossblood.org)
- A voluntary donor registry and a rare blood group registry are maintained to facilitate availability of blood donors as and when required. (tmckolkata.com)
Samples5
- Sample Population -50 blood samples obtained from 30 dogs. (avma.org)
- QIAamp DNA Blood Kits yield DNA sized from 200 bp to 50 kb, depending on the age and storage of samples (see figure Apoptotic banding in stored blood. Genomic DNA from 8 blood samples stored at 4°C for 1 week. DNA was purified using the QIAamp DNA Blood Mini Kit. When blood is stored at 4°C, the DNA is rapidly degraded due to apoptosis; the resulting apoptotic banding pattern can clearly be seen in these samples. M1 : lambda- HindIII ; M2 :100 bp ladder. "> "Apoptotic banding in stored blood" ). (qiagen.com)
- The QIAamp 96 DNA Blood Kit processes up to 200 µl sample size, with a preparation time of 192 samples in two to three hours, yielding highly pure DNA in less than one minute per preparation. (qiagen.com)
- One patient was excluded from the study due to haemolysis of blood samples. (who.int)
- Fifty blood samples were collected from HIV-positive children and processed for DBCSs. (elsevier.com)
Donor3
- There are so many reasons why someone may need blood that we don't even realize," said Sandra Ryden, donor recruitment account manager for the Red Cross. (redcrossblood.org)
- Simply download the American Red Cross Blood Donor App , visit RedCrossBlood.org or call 1-800-RED CROSS (1-800-733-2767) to make an appointment or for more information. (redcrossblood.org)
- A blood donor card or driver's license or two other forms of identification are required at check-in. (redcrossblood.org)
Haemolysis1
- However, over-vigorous collection of blood and deposition into a vacutainer may cause post collection haemolysis, although this rarely results in a low PCV. (vin.com)
Liver1
- 3 genotypes of PARV4 have been found to infect humans and-white colobus monkeys (buffy coat, liver, intestine, ( 6 , 7 ). (cdc.gov)
Purification1
- In 2021 after the Covid pandemic the guidelines for Sepsis have been changed significantly in terms of sepsis screening, diagnosis with qSOFA vs NEWS, MEWS or SIRS criteria, use of antibiotics, resuscitation including pressor support, IV fluids and use of steroids and blood purification techniques. (medscape.com)
Leukocytes1
- The current recommended method for diagnosing HIV-1 in newborns infected vertically and in adults, during the "window period", is the detection of proviral HIV-1 DNA within leukocytes (buffy coat). (elsevier.com)
Acute1
- A factor to remember in the animal that has had substantial acute blood loss is that PCV and TS do not fall immediately but take about 24 hours to reach a nadir. (vin.com)
Smears2
- Tick-borne relapsing fever (TBRF) is definitively confirmed in the laboratory with direct observation of spirochetes in manually inspected peripheral blood smears during episodes of fever. (medscape.com)
- Thick smears are more sensitive than thin smears and can detect one log fewer organisms (ie, 10 4 /mL of blood). (medscape.com)
Autologous1
- A 15 ml sample of autologous blood was collected from each animal through cannulation of the ear vein into a syringe containing 1.48 ml of 10% sodium citrate. (bvsalud.org)
Transfusions3
- through blood transfusions, contami- confirmed by immunoblot ( 3 ). (cdc.gov)
- Pretransplant blood transfusions improve kidney allograft survival by unknown mechanisms. (elsevier.com)
- Blood transfusions also stimulate anti-HLA production and delay transplantation for many patients. (elsevier.com)
Borrelia2
- In this study, we aimed to improve the methods currently used to find Borrelia in human blood, and identified two opportunities for optimization. (mdpi.com)
- Lyme Borreliosis (Borrelia burgdorferi) DNA is not typically detected in peripheral blood, even when Lyme disease is the cause of clinical signs. (tamu.edu)
Centers1
- This is one of the few NABH accredited Blood Centers in the country and hence high quality standards are practiced at all times. (tmckolkata.com)
White2
- The buffy coat layer is usually a white layer about 1 mm thick that sits above the RBCs and it is difficult to detect a leukocytosis unless it is profound. (vin.com)
- In addition, the data presented demonstrates the plant extract had the potential to improve the red and white blood cell indices reducing parasitaemia following T. b. brucei infection. (bvsalud.org)
Human3
- Babesiosis is transmitted to humans through the blood meal of Ixodes ticks, the same vector as for Lyme disease and human granulocytic anaplasmosis. (bmj.com)
- The aim of the present study was to determine the ability of fluoride-modified titanium surfaces to activate complement cascades using the human buffy coat as model. (uio.no)
- This protocol is simple, robust and highly consistent, providing a reliable methodology for the generation of human iPSCs from readily accessible 4 ml of blood. (stembook.org)
Detection1
- Detection of CMV DNA in the peripheral blood by PCR analysis may help identify these patients. (scirp.org)
Fractions1
- The system uses CCD camera technology, software and precision hardware to identify the three fractions of blood and calculate their exact positions within the sample tube. (kcl.ac.uk)
Tubes2
- From each patient, 5 ml of venous blood was collected in heparinized tubes. (who.int)
- This accessory allows the JANUS G3 Blood iQ Workstation to record and track downstream sample tubes. (perkinelmer.com)
Cell5
- Genes of red blood cell groups are highly polymorphic and the distribution of allelic frequencies in these systems varies between different regions of the world. (scielo.br)
- The red blood cell groups are composed of numerous antigens, of which the main ones are: ABO, Rh, Kell, Duffy, Kidd and MNS. (scielo.br)
- The Kell protein is associated with other proteins on the red blood cell membrane. (scielo.br)
- Draw 4 ml of peripheral blood into a BD Vacutainer CPT Cell Preparation Tube with sodium citrate. (stembook.org)
- Every two seconds in the United States blood is needed to respond to patient emergencies, including accident and burn victims, heart surgery and organ transplant patients, and those receiving treatment for leukemia, cancer or sickle cell disease. (redcrossblood.org)
Dilution1
- 1:1 dilution of blood with PBS was made. (ispub.com)
Sample1
- small sample size - ease of sample collection - ease of shipping (regular mail) - stability and low cost storage - offers a source for study of exogenous or endogenous compounds other than DNA - genotyping generally requires 10 ng/genotype, and with current technology as little as 2.5 ng per SNP so that scores to hundreds of genotypes could be obtained from one blood spot. (cdc.gov)
Urinalysis1
- Your veterinarian may recommend completing blood work, including a complete blood count, chemistry panel, and urinalysis, as well as tests to exclude similar conditions. (petplace.com)
Layer1
- Leukopenia cannot be detected by looking at the buffy coat layer. (vin.com)
Analysis3
- values obtained by use of impedance can be considered minimums, and values obtained by use of buffy coat analysis may be either minimum values or reasonable estimates of PC. (avma.org)
- Results: Retrospective analysis of peripheral blood by PCR analysis was positive for CMV DNA. (scirp.org)
- The blood components can be reformatted into nearly any consumable for downstream storage or analysis. (perkinelmer.com)
Genes1
- BACKGROUND: Red blood group genes are highly polymorphic and the distribution of alleles varies among different populations and ethnic groups. (scielo.br)
Spike2
- Spike patted his coat pocket for his smokes and then reached in and pulled out the crumpled pack. (freehostia.com)
- Buffy laid back and allowed Spike to take her fears away, clenching her eyes closed tightly. (darkstarfic.com)
Resist2
- And Buffy could not resist coming back for more. (darkstarfic.com)
- Anthony Roberie has countless advantages in this Zonia Guillemette, sildenafil tablets 50 mg reviews Marquis Paris is equal to the power of the entire Qinglong family, and even the power of side effects of Cialis 5 mg the Qinglong family When these powers erupt, how can Buffy Antes be able to resist? (bnew.bt)
Parasites1
- or = 100 parasites/ul of blood by QBC technique. (who.int)
Hospitals1
- Tata Medical Center (TMC) being one of the leading cancer hospitals in India, is one of the best Blood Centre in the country with the state-of-the-art facilities. (tmckolkata.com)