Blood buffy coat. Medical search

The fraction of a blood sample, following CENTRIFUGATION, that is distinguished as a thin light-colored layer between the RED BLOOD CELLS, underneath it, and the PLASMA, above it. It is composed mostly of WHITE BLOOD CELLS and PLATELETS.

Linear furanocoumarins which are found in many PLANTS, especially UMBELLIFERAE and RUTACEAE, as well as PSORALEA from which they were originally discovered. They can intercalate DNA and, in an UV-initiated reaction of the furan portion, alkylate PYRIMIDINES, resulting in PHOTOSENSITIVITY DISORDERS.

The transfer of blood platelets from a donor to a recipient or reinfusion to the donor.

Non-nucleated disk-shaped cells formed in the megakaryocyte and found in the blood of all mammals. They are mainly involved in blood coagulation.

The preparation of platelet concentrates with the return of red cells and platelet-poor plasma to the donor.

The attachment of PLATELETS to one another. This clumping together can be induced by a number of agents (e.g., THROMBIN; COLLAGEN) and is part of the mechanism leading to the formation of a THROMBUS.

A child whose needs, abilities, or other characteristics vary so much from the average in mental, physical, or social areas that a greater than usual level of services is needed to facilitate the child's maximum potential development.

Addition of methyl groups to DNA. DNA methyltransferases (DNA methylases) perform this reaction using S-ADENOSYLMETHIONINE as the methyl group donor.

Addition of methyl groups. In histo-chemistry methylation is used to esterify carboxyl groups and remove sulfate groups by treating tissue sections with hot methanol in the presence of hydrochloric acid. (From Stedman, 25th ed)

Blood of the fetus. Exchange of nutrients and waste between the fetal and maternal blood occurs via the PLACENTA. The cord blood is blood contained in the umbilical vessels (UMBILICAL CORD) at the time of delivery.

An operating division of the US Department of Health and Human Services. It is concerned with the overall planning, promoting, and administering of programs pertaining to health and medical research. Until 1995, it was an agency of the United States PUBLIC HEALTH SERVICE.

Areas of increased density of the dinucleotide sequence cytosine--phosphate diester--guanine. They form stretches of DNA several hundred to several thousand base pairs long. In humans there are about 45,000 CpG islands, mostly found at the 5' ends of genes. They are unmethylated except for those on the inactive X chromosome and some associated with imprinted genes.

A genetic process by which the adult organism is realized via mechanisms that lead to the restriction in the possible fates of cells, eventually leading to their differentiated state. Mechanisms involved cause heritable changes to cells without changes to DNA sequence such as DNA METHYLATION; HISTONE modification; DNA REPLICATION TIMING; NUCLEOSOME positioning; and heterochromatization which result in selective gene expression or repression.

An order of heteroxenous protozoa in which the macrogamete and microgamont develop independently. A conoid is usually absent.

Infections with unicellular organisms formerly members of the subkingdom Protozoa. The infections may be experimental or veterinary.

Diseases of birds not considered poultry, therefore usually found in zoos, parks, and the wild. The concept is differentiated from POULTRY DISEASES which is for birds raised as a source of meat or eggs for human consumption, and usually found in barnyards, hatcheries, etc.

A phylum of unicellular parasitic EUKARYOTES characterized by the presence of complex apical organelles generally consisting of a conoid that aids in penetrating host cells, rhoptries that possibly secrete a proteolytic enzyme, and subpellicular microtubules that may be related to motility.

Warm-blooded VERTEBRATES possessing FEATHERS and belonging to the class Aves.

A superfamily of nematodes of the suborder SPIRURINA. Its organisms possess a filiform body and a mouth surrounded by papillae.

Small uniformly-sized spherical particles, of micrometer dimensions, frequently labeled with radioisotopes or various reagents acting as tags or markers.

Central retinal artery and its branches. It arises from the ophthalmic artery, pierces the optic nerve and runs through its center, enters the eye through the porus opticus and branches to supply the retina.

The destruction of ERYTHROCYTES by many different causal agents such as antibodies, bacteria, chemicals, temperature, and changes in tonicity.

A chemical system that functions to control the levels of specific ions in solution. When the level of hydrogen ion in solution is controlled the system is called a pH buffer.

Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN.

The resistance that a gaseous or liquid system offers to flow when it is subjected to shear stress. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)

The study of the deformation and flow of matter, usually liquids or fluids, and of the plastic flow of solids. The concept covers consistency, dilatancy, liquefaction, resistance to flow, shearing, thixotrophy, and VISCOSITY.

Resistance and recovery from distortion of shape.

Process of using a rotating machine to generate centrifugal force to separate substances of different densities, remove moisture, or simulate gravitational effects. It employs a large motor-driven apparatus with a long arm, at the end of which human and animal subjects, biological specimens, or equipment can be revolved and rotated at various speeds to study gravitational effects. (From Websters, 10th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

The study of parasites and PARASITIC DISEASES.

White blood cells. These include granular leukocytes (BASOPHILS; EOSINOPHILS; and NEUTROPHILS) as well as non-granular leukocytes (LYMPHOCYTES and MONOCYTES).

Inorganic salts of sulfurous acid.

Measurement of the intensity and quality of fluorescence.

Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.

A heat stable DNA-DIRECTED DNA POLYMERASE from the bacteria Thermus aquaticus. It is widely used for the amplification of genes through the process of POLYMERASE CHAIN REACTION. EC 2.7.7.-.

In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.

Transcription of Alu DNA elements in blood cells of sporadic Creutzfeldt-Jakob disease (sCJD). (1/25)

Alu DNA elements were long considered to be of no biological significance and thus have been only poorly defined. However, in the past Alu DNA elements with well-defined nucleotide sequences have been suspected to contribute to disease, but the role of Alu DNA element transcripts has rarely been investigated. For the first time, we determined in a real-time approach Alu DNA element transcription in buffy coat cells isolated from the blood of humans suffering from sporadic Creutzfeldt-Jakob disease (sCJD) and other neurodegenerative disorders. The reverse transcribed Alu transcripts were amplified and their cDNA sequences were aligned to genomic regions best fitted to database genomic Alu DNA element sequences deposited in the UCSC and NCBI data bases. Our cloned Alu RNA/cDNA sequences were widely distributed in the human genome and preferably belonged to the "young" Alu Y family. We also observed that some RNA/cDNA clones could be aligned to several chromosomes because of the same degree of identity and score to resident genomic Alu DNA elements. These elements, called paralogues, have purportedly been recently generated by retrotransposition. Along with cases of sCJD we also included cases of dementia and Alzheimer disease (AD). Each group revealed a divergent pattern of transcribed Alu elements. Chromosome 2 was the most preferred site in sCJD cases, besides chromosome 17; in AD cases chromosome 11 was overrepresented whereas chromosomes 2, 3 and 17 were preferred active Alu loci in controls. Chromosomes 2, 12 and 17 gave rise to Alu transcripts in dementia cases. The detection of putative Alu paralogues widely differed depending on the disease. A detailed data search revealed that some cloned Alu transcripts originated from RNA polymerase III transcription since the genomic sites of their Alu elements were found between genes. Other Alu DNA elements could be located close to or within coding regions of genes. In general, our observations suggest that identification and genomic localization of active Alu DNA elements could be further developed as a surrogate marker for differential gene expression in disease. A sufficient number of cases are necessary for statistical significance before Alu DNA elements can be considered useful to differentiate neurodegenerative diseases from controls. (+info)

Methylprednisolone prevents tumour necrosis factor-alpha-dependent multinucleated giant cell formation. (2/25)

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The Mirasol Pathogen Reduction Technology system and quality of platelets stored in platelet additive solution. (3/25)

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Effects of storage time and leucocyte burden of packed and buffy-coat depleted red blood cell units on red cell storage lesion. (4/25)

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Development and validation of a new, sensitive immunochemical assay for O(6)-methylguanine in DNA and its application in a population study. (5/25)

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IL-4 and IL-13 alter plasmacytoid dendritic cell responsiveness to CpG DNA and herpes simplex virus-1. (6/25)

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Merkel cell polyomavirus DNA sequences in the buffy coats of healthy blood donors. (7/25)

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The histamine H4 receptor is highly expressed on plasmacytoid dendritic cells in psoriasis and histamine regulates their cytokine production and migration. (8/25)

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For blood samples, these include centrifugation followed by examination of the buffy coat; mini anion-exchange/centrifugation; ... The first uses dried blood, while the other two use whole blood samples. A 2002 study found the wb-CATT to be the most ... and the quantitative buffy coat (QBC) technique. For other samples, such as spinal fluid, concentration techniques include ... Diagnosis is by finding the parasite in a blood smear or in the fluid of a lymph node. A lumbar puncture is often needed to ...

https://en.wikipedia.org/wiki/African_trypanosomiasis

Using HCT trypanosomes can be detected in the blood even in field conditions. Buffy coat can be used to increase detection. ... such as wet blood film, and stained blood smears are used because so far, the best identifier is looking at the blood of the ... These blood sucking insects are Stomoxys, Haematobia (also known as Lyperosia), Haematopota, and Ornithordorus.[citation needed ... This means a good indicator of disease would be looking at trypanosome density in capillary blood and connective tissue. After ...

https://en.wikipedia.org/wiki/Surra

... separating the plasma from the red blood cells. These layers are divided by the buffy coat. The buffy coat consists of the ... The tube is snapped at the buffy coat and added to a slide for microscopic examination. Adding methylene blue stain to the ... Microfilarial detection is accomplished by the using one of the following methods: Direct blood smear A blood sample is ... A blood sample is mixed with 2% formalin and centrifuged in a tube. The supernatant is removed and methylene blue stain is ...

https://en.wikipedia.org/wiki/Dirofilaria_immitis

"Platelets, Pooled, Buffy Coat Derived, in Additive Solution and Plasma, Leucocyte Depleted". www.transfusionguidelines.org. ... Blood. 119 (23): 5553-5562. doi:10.1182/blood-2011-11-393165. ISSN 1528-0020. PMC 3369689. PMID 22496156. Petraszko, Tanya; ... Some blood banks maintain records of the estimated number of platelets in each unit. Current requirements in the US stipulate ... Canadian Blood Services. Estcourt, Lise J; Malouf, Reem; Hopewell, Sally; Trivella, Marialena; Doree, Carolyn; Stanworth, Simon ...

https://en.wikipedia.org/wiki/Platelet_transfusion_refractoriness

Motile organisms may be visible in the buffy coat when a blood sample is spun down. Serological testing is also common. The use ... Diagnosis relies on recognition of the flagellate on a blood smear. ... blood in urine, aching muscles and joints, headaches and irritability. In the first phase, the patient has only intermittent ...

https://en.wikipedia.org/wiki/Trypanosomiasis

... and the significance of the buffy coat in the shed blood". The Journal of Pathology and Bacteriology. 6 (3): 303-314. doi: ...

https://en.wikipedia.org/wiki/Samuel_George_Shattock

... infections by nested amplification of viral DNA in bovine blood buffy coat specimens". Journal of Veterinary Diagnostic ...

https://en.wikipedia.org/wiki/Alcelaphine_gammaherpesvirus_2

Starting material are for example, whole blood, blood serum, buffy coat, urine, feces, cerebrospinal fluid, sperm, saliva, ... The silica coated beads coated various magnetic particles (magnetic carrier) with silica are often used. Maghemite particle (γ- ... Today, the assay characterized by using silica coated magnetic beads seems to be the most common. Therefore, in this article, " ... How do SPRI beads work? DNA Extraction Methods For Large Blood Volumes B Vogelstein and D Gillespie;"Preparative and analytical ...

https://en.wikipedia.org/wiki/Boom_method

Quantitative buffy coat (QBC) is a laboratory test to detect infection with malaria or other blood parasites. The blood is ... In such cases the medical technologist may obtain a buffy coat, from which a blood smear is made. This smear contains a much ... The mainstay of malaria diagnosis has been the microscopic examination of blood, utilizing blood films. Although blood is the ... the fluorescing parasites can then be observed under ultraviolet light at the interface between red blood cells and buffy coat ...

https://en.wikipedia.org/wiki/Diagnosis_of_malaria

... a smear can be prepared from the buffy coat of the blood sample to concentrate the cells. Tumour cells in peripheral blood may ... The amount of tumour cells on the blood smear can range from 1 to 80 percent of the total white blood cell count, with lower ... could contribute to the presence of tumour cells in the blood. Carcinocythemia can be detected on a routine blood smear ... Tumour cells are often found at the edge of the blood smear due to their large size, so this area should be examined thoroughly ...

https://en.wikipedia.org/wiki/Carcinocythemia

... buffy coat (middle, thin white layer) and erythrocyte layer (bottom, red layer) can be seen. Blood circulation: Red = ... mixed-blood and blood relative. Autotransfusion Blood as food Blood pressure Blood substitutes ("artificial blood") Blood test ... of blood is blood plasma, a fluid that is the blood's liquid medium, which by itself is straw-yellow in color. The blood plasma ... Blood that has a pH below 7.35 is too acidic, whereas blood pH above 7.45 is too basic. Blood pH, partial pressure of oxygen ( ...

https://en.wikipedia.org/wiki/Blood

He used the concept of buffy coat as the starting point of his work on red cell sedimentation and the more general problem of ... The Fåhræus effect is the decrease in average concentration of red blood cells in human blood as the diameter of the glass tube ... Considering steady laminar fully developed blood flow in a small tube with radius of r 0 {\displaystyle r_{0}} , whole blood ... c) The migration of blood cells from the tube wall to the axis depends on the particle size and not on the particle density. (d ...

https://en.wikipedia.org/wiki/Fåhræus_effect

"In Vitro Evaluation of Buffy Coat Derived Platelet Concentrates in SSP+ Platelet Storage Medium". Transfusion Medicine. 16: 26 ... Blood bank#History (history of blood donation) Blood donation restrictions on men who have sex with men Blood substitute James ... Blood is something we all expect to be there for us when we need it, yet only 4% of us give blood. "Canadian Blood Services". ... "Facts About Blood". America's Blood Centers. Archived from the original on 2013-11-08. Retrieved 2013-01-04. "Give Blood". NHS ...

https://en.wikipedia.org/wiki/Blood_donation

... see Blood plasma fractionation), the buffy coat, which is a thin layer of leukocytes (white blood cells) mixed with platelets ... when centrifuged the silicone gel forms a layer on top of the buffy coat, allowing the blood serum to be removed more ... Blood fractionation is the process of fractionating whole blood, or separating it into its component parts. This is typically ... Blood plasma fractionation Blood Plasma Pooling (from the Bloodbook.com website) Statement by Dr. Kathryn Zoon, Food and Drug ...

https://en.wikipedia.org/wiki/Blood_fractionation

Blood sampling follows a strict quality protocol, collecting serum, plasma, buffy coat, immortalized cells for cell line ... collecting blood and urine samples and by clinical examination. In connection with the HUNT3 survey (2006-2008), HUNT Biobank ...

https://en.wikipedia.org/wiki/HUNT_Biobank

... after which three major blood components can be visualized: plasma, buffy coat and erythrocytes(blood cells). These separated ... The process of blood fractionation involves separation of blood into its main components. Blood fractionation refers generally ...

https://en.wikipedia.org/wiki/Fractionation

"The efficacy of the ultraviolet C pathogen inactivation system in the reduction of Babesia divergens in pooled buffy coat ... Zintl, A; Mulcahy, G; Skerrett, HE; Taylor, SM; Gray, JS (2003). "Babesia divergens, a Bovine Blood Parasite of Veterinary and ... To Invade the Human Red Blood Cell". Infection and Immunity. 73 (1): 649-651. doi:10.1128/IAI.73.1.649-651.2005. PMC 538995. ...

https://en.wikipedia.org/wiki/Babesia_divergens

White cells are found in the buffy coat, a thin, typically white layer of nucleated cells between the sedimented red blood ... The complete blood cell count is a blood panel that includes the overall WBC count and the white blood cell differential, a ... All white blood cells have nuclei, which distinguishes them from the other blood cells, the anucleated red blood cells (RBCs) ... Histamine is responsible for widening blood vessels and increasing the flow of blood to injured tissue. It also makes blood ...

https://en.wikipedia.org/wiki/White_blood_cell

... a layer of mono-nuclear cells called buffy coat (PBMC/MNC), Ficoll-Paque, and erythrocytes & granulocytes which should be ... These products allow blood to be layered on much more quickly without mixing polysaccharide and blood. An example of such a ... This allows the blood sample to be rapidly pipetted onto the insert, avoiding the need for overlaying it directly onto Ficoll- ... Major blood clotting may sometimes occur in the PBMC layer. Ethylene diamine tetra-acetate (EDTA) and heparin are commonly used ...

https://en.wikipedia.org/wiki/Ficoll

Buffy-coat preparations of peripheral blood or aspirates from marrow, spleen, lymph nodes, or skin lesions should be spread on ... The visceral form starts with skin ulcers and later presents with fever, low count of red blood cells, and enlarged spleen and ... Additionally, visceral disease can be diagnosed by blood tests. Leishmaniasis can be partly prevented by sleeping under nets ... Amastigotes are seen within blood and spleen monocytes or, less commonly, in circulating neutrophils and in aspirated tissue ...

https://en.wikipedia.org/wiki/Leishmaniasis

The layer between the red cells and the plasma is referred to as the buffy coat and is sometimes removed to make platelets for ... A blood bank is a center where blood gathered as a result of blood donation is stored and preserved for later use in blood ... Several types of blood transfusion exist: Whole blood, which is blood transfused without separation. Red blood cells or packed ... For blood donation agencies in various countries, see List of blood donation agencies and List of blood donation agencies in ...

https://en.wikipedia.org/wiki/Blood_bank

Specifically, buffy coat (WBC + platelets) is separated from whole blood, chemically treated with 8-methoxypsoralen (instilled ... Blood irradiation therapy ICD10 Procedure code: this was assigned based on cross-walking from the ICD9 code at ICD10DAta.com ... is a form of apheresis and photodynamic therapy in which blood is treated with a photosensitizing agent and subsequently ...

https://en.wikipedia.org/wiki/Photopheresis

... buffy coat (used to make platelets), and red blood cells. The third method is sedimentation: the blood simply sits overnight ... Whole blood is made up of red blood cells, white blood cells, platelets, and blood plasma. It is best within a day of ... Historically, blood was transfused as whole blood without further processing. Most blood banks now split the whole blood into ... Whole blood (WB) is human blood from a standard blood donation. It is used in the treatment of massive bleeding, in exchange ...

https://en.wikipedia.org/wiki/Whole_blood

... "buffy coat" layer, which includes the platelets and the white blood cells. The "buffy coat" is isolated in a sterile bag, ... On a stained blood smear, platelets appear as dark purple spots, about 20% the diameter of red blood cells. The smear is used ... Although thrombosis, blood coagulation in intact blood vessels, is usually viewed as a pathological immune response, leading to ... Apheresis platelets are collected using a mechanical device that draws blood from the donor and centrifuges the collected blood ...

https://en.wikipedia.org/wiki/Platelet

Blood Buffy Coat at the US National Library of Medicine Medical Subject Headings (MeSH) Buffy Coat Isolation for Manual Ficoll ... The layer beneath the buffy coat contains granulocytes and red blood cells. The buffy coat is commonly used for DNA extraction ... "A preliminary comparative report of quantitative buffy coat and modified quantitative buffy coat with peripheral blood smear in ... The buffy coat is the fraction of an anticoagulated blood sample that contains most of the white blood cells and platelets ...

https://en.wikipedia.org/wiki/Buffy_coat

The end product in most cases is the classic sedimented blood sample with the RBC's at the bottom, the buffy coat of platelets ... To stop the blood from coagulating, anticoagulant is automatically mixed with the blood as it is pumped from the body into the ... Blood does not contact the device and during the separation, blood does not exit the kit. Reinfusion - At the end of the ... Blood taken from a healthy donor can be separated into its component parts during blood donation, where the needed component is ...

https://en.wikipedia.org/wiki/Apheresis

... "buffy coat" layer, which includes the platelets and the white blood cells. The "buffy coat" is isolated in a sterile bag, ... American Association of Blood Banks (2003). "5.1.5.1". Standards for Blood Banks and Transfusion Services (22nd ed.). Bethesda ... On a stained blood smear, platelets appear as dark purple spots, about 20% the diameter of red blood cells. The smear is used ... Although thrombosis, blood coagulation in intact blood vessels, is usually viewed as a pathological immune response, leading to ...

https://en.wikipedia.org/wiki/Thrombocytopathy

Donor lymphocyte (or leukocyte) infusion (DLI) or buffy coat infusion is a form of adoptive immunotherapy used after ... These donated white blood cells contain cells of the immune system that can recognize and destroy cancer cells. The goal of ... Luznik L, Fuchs EJ (2002). "Donor lymphocyte infusions to treat hematologic malignancies in relapse after allogeneic blood or ...

https://en.wikipedia.org/wiki/Donor_lymphocyte_infusion

Platelet-rich plasma (PRP) is widely used in North America and buffy coat (BC) is more widely used in Europe. Platelets are the ... Since it takes up to 3 liters of whole blood (the amount of a dozen blood bags) to generate a dose of platelets, white blood ... Blood accounts for about 8% of body weight, so a 50 kg (110 lb) donor has about four liters of blood. No more than 50% of a ... Even though red blood cells can also be collected in the process, many blood donation organizations do not do so because it ...

https://en.wikipedia.org/wiki/Plateletpheresis

White cells are found in the buffy coat, a thin, typically white layer of nucleated cells between the sedimented red blood ... All white blood cells have nuclei, which distinguishes them from the other blood cells, the anucleated red blood cells (RBCs) ... Histamine is responsible for widening blood vessels and increasing the flow of blood to injured tissue. It also makes blood ... "Blood. 96 (13): 4028-38. PMID 11110670.. *^ a b c d e f Kumar V, et al. (2010). Robbins and Cotran pathologic basis of disease ...

https://en.wikipedia.org/wiki/Immune_cell

The layer between the red cells and the plasma is referred to as the buffy coat and is sometimes removed to make platelets for ... A blood bank is a center where blood gathered as a result of blood donation is stored and preserved for later use in blood ... Types of blood transfusedEdit. Whole blood or blood with RBC, is transfused to patients with anaemia/iron deficiency. It also ... For blood donation agencies in various countries, see List of blood donation agencies and List of blood donation agencies in ...

https://en.m.wikipedia.org/wiki/Blood_bank

It can be detected by microscopic examination of fresh anticoagulated blood, or its buffy coat, for motile parasites; or by ... Screening of donated blood, blood components, and solid organ donors, as well as donors of cells, tissues, and cell and tissue ... T. cruzi can also be transmitted through blood transfusions. With the exception of blood derivatives (such as fractionated ... blood supply.[38][39] The Chagas Biovigilance Network reports current incidents of Chagas-positive blood products in the United ...

https://en.wikipedia.org/wiki/Chaga's_disease

According to his daughter, he usually "sweated blood" to write his songs.[45] Annie Get Your Gun is considered to be Berlin's ... "All the little writer could do was to finger the buttons on his coat while tears ran down his cheeks-in a vaudeville house!"[20 ... Music: Jack Nitzsche and Buffy Sainte-Marie. *Lyrics: Will Jennings (1982). *"Flashdance... What a Feeling" *Music: Giorgio ...

https://en.wikipedia.org/wiki/Irving_Berlin

If blood can get in, but cannot get out because one of the veins has been blocked, that part of the body turns purple. ... By the 1990s, references to bondage could be found in mainstream prime-time television series such as Buffy the Vampire Slayer ... A leather dog mask worn over a latex coat.. Products made of leather are often used in bondage because of their flexibility and ... Other dangers include nerve compression, circulation problems and fainting due to increase in blood pressure.[15][16] ...

https://en.wikipedia.org/wiki/Bondage_

... mayayakit la reng mangaputing cell king Buffy coat, metung a maimpis a tumpak (layer) da reng cell king pilatan da reng ... Blood values. Diameter (μm)[3]. Main targets[4]. Nucleus[4]. Granules[4]. Lifetime[3] ... Melalang ya ing katayang "korpuskulos a maputi" o "White Blood Cbgfgmjdghfell" uling kaibat ning centrifugation ning daya, ...

https://pam.wikipedia.org/wiki/Leukocyte

https://en.m.wikipedia.org/wiki/White_blood_cells

... "buffy coat" layer, which includes the platelets and the white blood cells. The "buffy coat" is isolated in a sterile bag, ... American Association of Blood Banks (2003). "5.1.5.1". Standards for Blood Banks and Transfusion Services (22nd ed.). Bethesda ... Platelets, also called thrombocytes (thromb- + -cyte, "blood clot cell"), are a component of blood whose function (along with ... On a stained blood smear, platelets appear as dark purple spots, about 20% the diameter of red blood cells. The smear is used ...

https://en.m.wikipedia.org/wiki/Platelet

They are smallish birds with tails that are usually long and pointed, and tend to be drab brownish or buffy all over. Striated ... Gallus gallus Blood pheasant, Ithaginis cruentus Himalayan monal, Lophophorus impejanus Snow partridge, Lerwa lerwa Satyr ... and feathers that are excellent at shedding water due to an oily coating. Fulvous whistling-duck, Dendrocygna bicolor (A) ...

https://en.wikipedia.org/wiki/List_of_birds_of_Nepal

... a color often used in description of birds Buffy (dog), Russian President Vladimir Putin's dog Buffy coat, a component of blood ... was nicknamed Buffy or Buffie or Buff Buffy (rapper) (born Kim Ju-hyeon, 1995) member of South Korean band MADTOWN Buffy ... the title character in the Buffy the Vampire Slayer media Buffy Gilmore, a main character in Scary Movie Buffy, the sister-in- ... Buffy is a pet form of the female given name Elizabeth. Buffy or Buffie may also refer to: Buffie Carruth (born 1977), American ...

https://en.wikipedia.org/wiki/Buffy

... using the part-fairy blood of Danielle's son Jakub - Danielle having some fairy blood in her from her mother's side of the ... Before declaring Sidney free, the Snow Queen advises Sidney to get a warm coat since it is "going to get cooler around here." ... voiced by Buffy Sainte-Marie) is a shiny flat rock. It told Sly Fox that it was the fairest in the land until White Snow came ...

https://en.wikipedia.org/wiki/Magic_Mirror_(Snow_White)

They are smallish birds with tails that are usually long and pointed, and tend to be drab brownish or buffy all over. ... and feathers that are excellent at shedding water due to an oily coating. Spotted whistling-duck, Dendrocygna guttata Plumed ... Gallus varius Blood pheasant, Ithaginis cruentus Himalayan monal, Lophophorus impejanus Sclater's monal, Lophophorus sclateri ... Ketupa flavipes Buffy fish-owl, Ketupa ketupu Northern hawk owl, Surnia ulula Eurasian pygmy-owl, Glaucidium passerinum ...

https://en.wikipedia.org/wiki/List_of_birds_of_Asia

The Buffy the Vampire Slayer novel Go Ask Malice: A Slayer's Diary depicts maenads as corrupted human beings in service of the ... Maenads are the adopted symbol of Tetovo in North Macedonia, depicted prominently of the city's coat of arms. The inclusion of ... Having symbolically eaten his body and drunk his blood, the celebrants became possessed by Dionysus. "Maenads" are found in ... Charlaine Harris' The Southern Vampire Mysteries novels and its television adaptation, HBO's True Blood (2nd season, aired in ...

https://en.wikipedia.org/wiki/Maenad

Blowing Lake Blowout Lake Bloxham Lake Bludgeon Lake Blue Cedar Lake Blue Chalk Lake Blue Coat Lakes Blue Goose Lake Blue Hawk ... Blithfield Long Lake Blob Lake Bloch Lake Block Lake Block's Pond Blockville Lake Blonde Lake Blondin Lake Blood Lake Bloody ... Budworm Lake Buell Lake Buells Creek Reservoir Buff Lake Buffalo Island Lake Buffalo Lake Buffington Lake Bufflehead Lake Buffy ... Lake Blowdown Lake Blowdown Lake Blowing Lake Blowout Lake Bloxham Lake Bludgeon Lake Blue Cedar Lake Blue Chalk Lake Blue Coat ...

https://en.wikipedia.org/wiki/List_of_lakes_of_Ontario:_B

EJ Gage, who plays Mordar the Bentback, also played one of the movers in the Buffy the Vampire Slayer episode "Buffy vs. ... As he sets off to find her, the trial begins and with no Champion and no Coat of Arms, Jo is told her life is forfeit, but ... As seen in "Orpheus," "Mandy" was playing on a diner's jukebox when Angel, circa 1976, fed upon a dying man's blood; Angel felt ... When he persists she asks him to help her find the "Coat of Arms" which might convince the Tribunal to call off whatever ...

https://en.wikipedia.org/wiki/Judgment_(Angel)

... best known for his work on Buffy the Vampire Slayer at the time, played Peter Salter. Despite his status as a former Buffy ... candy-coated escapism and romanticism of Alias, or the white-knuckled, thrill-a-minute intensity of 24. [Spooks] is its own ... but lost to Peter Bowker's work on television film Flesh and Blood. In the Craft awards, Julian Slater, Nigel Heath, Michael ...

https://en.wikipedia.org/wiki/Spooks_(series_1)

... is to combine the buffy coats (the fraction of blood containing white blood cells) from multiple routine blood donations to ... 297 The donor blood undergoes leukapheresis, a process by which white blood cells are separated from red blood cells and plasma ... During the blood collection, the donor's blood is mixed with hydroxyethyl starch, which helps separate the granulocytes from ... They are required to meet the general criteria for blood donation. Because the granulocyte concentrate also contains red blood ...

https://en.wikipedia.org/wiki/Granulocyte_transfusion

During a close-up, he sees that he's wearing the coins in his coat and then Hitler reveals himself as a Blutbad. The episode ... I can think of many different shows that have used this different structure to brilliant effect - the Buffy bottle episode ' ... Blue Bloods, and Undercover Boss. This was a 10% increase in viewership from the previous episode, which was watched by 4.79 ...

https://en.wikipedia.org/wiki/Three_Coins_in_a_Fuchsbau

She consoled him, drained him of blood and made him drink of her blood, thus transforming him into a vampire - "siring" him, in ... When the coat was destroyed by a bomb from the Immortal in Italy, Spike heartbrokenly declared it to be irreplaceable; but the ... Dracula". Buffy the Vampire Slayer. Season 5. Episode 1. WB. CS1 maint: discouraged parameter (link) "A Buffy Bestiary" Buffy ... Lies My Parents Told Me Fool_for_Love_(Buffy_the_Vampire_Slayer) Fool_for_Love_(Buffy_the_Vampire_Slayer) Intervention_(Buffy_ ...

https://en.wikipedia.org/wiki/Spike_(Buffy_the_Vampire_Slayer)

No yellow or buffy highlights on the sides where the darker upper body fur meets the lighter underbody fur. [Ears small.] → ... The fur on the abdomen is grey, and the coat colour turns grey during moulting. Phodopus species display a variety of ... Feoktistova, N. Yu.; Kropotkina, M. V.; Naidenko, S. V. (2010). "Seasonal Changes of Steroid Levels in Blood Plasma of Three ... May have yellow or buffy highlights on the sides where the darker upper body fur meets the lighter under body fur. [Ears larger ...

https://en.wikipedia.org/wiki/Phodopus

... which backfired in its first use because he forgot to take the warm-blooded/cold-blooded conflict into account when switching ... Muffy, Buffy and Fluffy (all three voiced by Kristen Schaal) are three adorable, cute, and cuddly rabbits who reside in the ... He is bald and wears a white lab coat. Skipper often voices dislike of him because of his use of needles and when injured, he ... It is revealed at the end of the contest that he only wants to make Mort turn to evil, as is in his blood, and help him fulfill ...

https://en.wikipedia.org/wiki/List_of_Madagascar_(franchise)_characters

Buffy coat is the component of whole blood that contains leukocytes an...,Genomic,DNA,Extraction,from,Buffy,Coat,Using,the, ... Perfect,gDNA,Blood,,,,,,,,,,,,Mini,Kit,biological,advanced biology technology,biology laboratory technology,biology device ... The versatile Perfect gDNA Blood Mini Kit allows the researcher to iso... ... Laura Pollock ...The Perfect gDNA Blood Mini Kit is an efficient way to purify genomic ... Introduction ... ...

http://www.bio-medicine.org/biology-technology/Genomic-DNA-Extraction-from-Buffy-Coat-Using-the-Perfect-gDNA-Blood--0D-0A-0A--------Mini-Kit-486-1/

BUFFY COAT (HG-BCD) reagent with the ReliaPrep™ Blood gDNA Miniprep System. ... we examine the compatibility of buffy coat DNA preserved in HEMAgene™• ... HEMAgene™•BUFFY COAT (HG-BCD) Preservative Compatibility with ReliaPrep™ Blood gDNA Miniprep System Application Note. ... HEMAgene BUFFY COAT HG BCD Preservative Compatibility with ReliaPrep Blood gDNA Miniprep System AN281PDF. (. 342. KB. ). - ...

https://www.promega.com/resources/pubhub/applications-notes/hemagene-buffy-coat-preservative-compatibility-with-reliaprep-blood-gdna-miniprep-system/

Comparative Study of Modified Quantitative Buffy Coat and Two Rapid Tests in Comparison with Peripheral Blood Smear in Malaria ... and modified QBC was done in comparison with conventional blood smear microscopy. One hundred patients infected with P. vivax ...

https://www.hindawi.com/journals/jpr/2014/194651/abs/

The double dose buffy coat method requires fewer buffy coats and reduces the use of consumables by up to 50% (e.g. pooling sets ... INTERCEPT Blood System, amotosalen, UVA, platelet, blood processing, buffy coat, IBS, transfusion HSV kategori Identifikatorer ... Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System. Abedi, ... is prepared by pooling the buffy coat layer from up to six whole blood donations. A procedure for producing "double dose" whole ...

http://oru.diva-portal.org/smash/record.jsf?pid=diva2%3A1106843&c=102&searchType=SIMPLE&language=no&query=&af=%5B%5D&aq=%5B%5B%5D%5D&aq2=%5B%5B%5D%5D&aqe=%5B%5D&noOfRows=50&sortOrder=author_sort_asc&sortOrder2=title_sort_asc&onlyFullText=false&sf=all

This BCTB blood sample subcollection consists of buffy-coat samples. A buffy coat contains white blood cells and platelets. ... This BCTB blood sample subcollection consists of buffy-coat samples. A buffy coat contains white blood cells and platelets.&rft ... BCTB Blood Buffy Coat Samples&rft.identifier=abctb.org.au 13&rft.publisher=Breast Cancer Tissue Bank&rft.description= ...

https://researchdata.ands.org.au/bctb-blood-buffy-coat-samples/60178

... while centrifuging whole blood separates red blood cells, generating a white blood cell buffy coat. Both sample types are used ... while centrifuging whole blood separates red blood cells, generating a white blood cell buffy coat. Both sample types are used ... while centrifuging whole blood separates red blood cells, generating a white blood cell buffy coat. Both sample types are used ... while centrifuging whole blood separates red blood cells, generating a white blood cell buffy coat. Both sample types are used ...

https://jhu.pure.elsevier.com/en/publications/cord-blood-buffy-coat-dna-methylation-is-comparable-to-whole-cord

We compared this technique with the thick blood smear diagnosis in a study of the efficacy of chloroquine therapy in Zaire. A ... Recently, a new direct diagnosis technique, the Quantitative Buffy Coat (QBC®) * Malaria Diagnosis System, has been developed. ... Chloroquine resistance was detected in 38% of the subjects by thick blood smear and in 45% by the QBC test. Of greater interest ... an average of 17 min was required to examine microscopic fields with 1,000 leukocytes by thick blood smear analysis compared ...

http://www.ajtmh.org/content/journals/10.4269/ajtmh.1992.47.446

... whereas the number of positive readings assessed by means of stained blood films fell significantly at dilutions below 3,263 ... In the present study, we used acridine orange-coated quantitative buffy coat (QBC) tubes, centrifugation, and fluorescence ... louse-borne relapsing fever is commonly made on the basis of the detection of Borrelia spirochetes on Giemsa-stained thin blood ... In the present study, we used acridine orange-coated quantitative buffy coat (QBC) tubes, centrifugation, and fluorescence ...

http://www.ajtmh.org/content/journals/10.4269/ajtmh.2001.65.164

The quantitative buffy coat (QBC) is a technique that is as sensitive as thick smears. Because results cannot be used to ... These red blood cells (RBCs) are sequestered in the spleen when malaria proteins, called sequestrins, on the RBC surface bind ... The quantitative buffy coat (QBC) is a technique that is as sensitive as thick smears. Because results cannot be used to ... What is the role of the quantitative buffy coat (QBC) technique in the diagnosis of malaria?. Updated: Apr 26, 2019 ...

https://www.medscape.com/answers/221134-40824/233913-overview

... tested and described the buffy coat method (BCM) for quick diagnostics (~ 20 min/sample) of avian blood parasites. Blood of 345 ... and each sample was examined using ME of stained blood films and the buffy coat, which was examined after centrifugation in ... BCM is a useful tool for diagnostics of blood parasite co-infections. Its application might be extended to studies of blood ... However, fixation, drying and staining of blood films as well as their ME are relatively time-consuming. This limits the ...

https://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-020-3984-8

Whole Blood Columns and Whole Blood Column Elution Buffer are included in the kit to allow direct processing of an entire buffy ... Buffy Coat CD4 MicroBead Kit has been developed for the rapid positive selection of CD4+ cells directly from one buffy coat ... One entire buffy coat is labeled with StraightFrom. ® Buffy Coat CD4 MicroBeads and loaded onto Whole Blood Columns, which are ... One entire buffy coat is labeled with StraightFrom. ® Buffy Coat CD4 MicroBeads and loaded onto Whole Blood Columns, which are ...

https://www.miltenyibiotec.com/FI-en/products/straightfrom-buffy-coat-cd4-microbead-kit-human.html

Whole blood. (anticoagulated or blood clots). Buffy coat. 100 - 400 μg/10 mLc. ~200 μg/mLc - relatively low-cost storage (-80° ... Blood Spots. 12 - 42 ng/μL (adults)a. 43 - 78 ng/μL (neonates)a. 1/4 inch punch from 75 μL volume yields about 12 μL of bloodb ... with current technology as little as 2.5 ng per SNP so that scores to hundreds of genotypes could be obtained from one blood ...

https://www.cdc.gov/genomics/resources/books/huge/tables/chap05_tables.htm

Preparation of buffy coat. *Centrifuge the blood specimen at 2,500 x g for 10 minutes at room temperature. ... Erythrocytes can be removed through the use of a hypotonic buffer or by using gradient centrifugation to create a buffy coat in ... The light tan middle layer is the buffy coat and contains concentrated leukocytes. The bottom red layer contains erythrocytes. ... Removal of eukaryotes from bloodIf DNA is being extracted from clinical specimens such as blood, CSF, or serum, considerations ...

http://www.cdc.gov/meningitis/lab-manual/chpt10-pcr.html

The morphology of buffy coat from cord blood of normal human newborns was investigated and the results were compared with our ... P. EFRATI, L. ROZENSZAJN, E. SHAPIRA; The Morphology of Buffy Coat from Cord Blood of Normal Human Newborns. Blood 1961; 17 (4 ... The Morphology of Buffy Coat from Cord Blood of Normal Human Newborns P. EFRATI, P. EFRATI ... The morphology of buffy coat from cord blood of normal human newborns was investigated and the results were compared with our ...

https://ashpublications.org/blood/article/17/4/497/37367/The-Morphology-of-Buffy-Coat-from-Cord-Blood-of

... automated method for extraction of genomic DNA from 50-250µl of buffy coat samples using the Maxwell RSC Instrument. ... The Maxwell RSC Buffy Coat DNA Kit is a simple, ... Maxwell® RSC Whole Blood DNA Kit. Extracts DNA from whole blood ... The Maxwell® RSC Buffy Coat DNA Kit is a simple, automated method for extraction of genomic DNA from 50-250µl of buffy coat ... Automated DNA Purification from Buffy Coat Samples. *Purifies high yields of DNA from 50-250µl of buffy coat samples in about ...

https://www.promega.com/products/nucleic-acid-extraction/genomic-dna/maxwell-rsc-buffy-coat-dna-kit/

... and clinical studies related to all aspects of blood transfusion. ... Journal of Blood Transfusion is a peer-reviewed, Open Access ... "Comparison of blood component preparation methods from whole blood bags based on buffy coat extraction," Transfusion and ... C. H. Mondéjar, S. Bonanad, M. A. Soler et al., "Quality analysis of blood components obtained by automated buffy-coat layer ... of blood components separated from donated whole blood after an overnight holding at room temperature with the buffy coat ...

https://www.hindawi.com/journals/jbt/2013/154838/ref/

Blood. *EDTA plasma. *DSMO (10%) whole blood. *Buffy coat. *Peripheral blood mononuclear cells ... Step 2: A general inventory of blood and cardiovascular-related tissues will be posted on the Cardiovascular Centers InfoScope ...

https://www.mcw.edu/departments/cardiovascular-center-heart/research/cvc-tissue-bank

Blood Plasma. The Buffy Coat. 55% 45%. Blood Plasma. Cellular Components. The Cellular Components. Blood Facts. Blood makes up ... The buffy coat is made up of white blood cells and platelets.. This makes up less than 1% of the total blood volume.. There are ... Buffy Coat. Platelets and INR. eGFR and Creatinine. Blood plasma is the straw coloured liquid component of blood.. 91% is water ... and therefore AB plasma is given to patients with any Blood type. Human Blood; red Blood cells, white Blood cells, plasma and ...

https://prezi.com/iddhwuaiehu4/deranged-blood-results/

... red blood cells (RBC), buffy coat, plasma, serum, DNA and RNA that derived from blood in human biobanks. These biological ... Repository of Human Blood Derivative Biospecimens in Biobank: Technical Implications.. Mohamadkhani A1, Poustchi H1. ... This review overview the significance and storage condition of biospecimens including whole blood, ...

https://www.ncbi.nlm.nih.gov/pubmed/26106464

Leukocyte count in association with whole blood culture, whole blood PCR, buffy coat culture and buffy coat PCR - The average ... In conclusion, buffy coat PCR, buffy coat culture and whole blood PCR can be used as alternative methods to whole blood culture ... Comparison of buffy coat PCR with whole blood culture - The buffy coat PCR showed that 47 of the 114 specimens (or 41.2%) were ... whole blood culture and buffy coat PCR, buffy coat culture or whole blood PCR. A Kappa coefficient of 0.8-1.0 indicates a very ...

http://memorias-old.ioc.fiocruz.br/issues/past-issues/item/314-evaluation-of-buffy-coat-16s-rrna-pcr-buffy-coat-culture-and-whole-blood-pcr-for-detection-of-bacteraemia

detection, Giemsa-stained blood smear. +§. +§. +§. +¶. Quantitative buffy coat. ND. ND. ND. +. ELISA anti-B. murgdorferi (titer ... First sample was positive after review prompted by the quantitative buffy coat result.. #Siemens, Erlangen, Germany.. **Bio- ...

https://wwwnc.cdc.gov/eid/article/19/2/12-1325-t1.htm

... rich plasma and buffy coats using a new technique with 111 In and 51 Cr, Transfusion" on DeepDyve, the largest online rental ... 70% of whole blood). WBCs remaining in the BC‐PC averaged 0.19 × 108 per unit compared to 3.6 × 108 per unit for PRP‐PC. Buffy ... 70% of whole blood). WBCs remaining in the BC‐PC averaged 0.19 × 108 per unit compared to 3.6 × 108 per unit for PRP‐PC. Buffy ... Paired comparison of platelet concentrates prepared from platelet‐rich plasma and buffy coats... Keegan, T.; Heaton, A.; Holme ...

https://www.deepdyve.com/lp/wiley/paired-comparison-of-platelet-concentrates-prepared-from-platelet-rich-BYL6FX17E6?impressionId=5d62d07227c95&i_medium=docview&i_campaign=references&i_source=references

https://en.wikipedia.org/wiki/African_trypanosomiasis

Here we validated a novel procedure for ambient temperature preservation of DNA in white blood cell and buffy coat lysates ... High DNA stability in white blood cells and buffy coat lysates stored at ambient temperature under anoxic and anhydrous ... High DNA stability in white blood cells and buffy coat lysates stored at ambient temperature under anoxic and anhydrous ... High DNA stability in white blood cells and buffy coat lysates stored at ambient temperature under anoxic and anhydrous ...

http://www.imagene.eu/high-dna-stability-white-blood-cells-and-buffy-coat-lysates-stored-at-ambient-temperature-under-anoxic-and-anhydrous-atmosphere/

... cancer cells from the undiluted and untreated buffy coat blood samples. The first capture layer consisted of a GF/D glass fiber ... cancer cells from the undiluted and untreated buffy coat blood samples. The first capture layer consisted of a GF/D glass fiber ... cancer cells from the undiluted and untreated buffy coat blood samples. The first capture layer consisted of a GF/D glass fiber ... cancer cells from the undiluted and untreated buffy coat blood samples. The first capture layer consisted of a GF/D glass fiber ...

https://arizona.pure.elsevier.com/en/publications/smartphone-based-on-chip-fluorescence-imaging-and-capillary-flow-

quantitative buffy coat capillary tubes versus thin and thick blood films in the diagnosis of malaria in Saudi Arabia ... The, quantitative buffy coat capillary tubes versus thin and thick blood films in the dia ... The quantitative buffy coat [QBC] capillary tube was compared with both the thin and thick films in the diagnosis of malaria ... Index: IMEMR (Eastern Mediterranean) Main subject: Blood Chemical Analysis / Laboratories Limits: Humans Language: English ...

https://search.bvsalud.org/gim/resource/en/emr-48269

Present study was undertaken to compare and study the efficacy of peripheral blood smear (PBS), modified centrifuged buffy coat ... Key words Malaria, Peripheral blood smear, centrifuged buffy coat smear, Rapid malaria antigen detection test ... Present study was undertaken to compare and study the efficacy of peripheral blood smear (PBS), modified centrifuged buffy coat ... A Prospective Comparative Study of Peripheral Blood Smear, Modified Centrifuged Buffy Coat Smear and Rapid Malaria Antigen ...

https://www.ijcmas.com/abstractview.php?ID=14900&vol=8-11-2019&SNo=8

Stained smears of buffy coats were 83% and 100% respectively. A.O. stained smear of buffy coat, as a diagnostic test of sepsis ... Comparison of blood culture with acridine orange stained smear of buffy coat in diagnosis of septicemia] ... Sensitivity and specificity of blood cultures were 26% and 92% respectively. A.O. stained smears of buffy coats were positive ... Comparison of blood culture with acridine orange stained smear of buffy coat in diagnosis ...

https://search.bvsalud.org/gim/resource/en/emr-83698

https://en.wikipedia.org/wiki/Surra

Buffy coat is the component of whole blood that contains leukocytes and platelets and makes up only 1% of the entire whole blood composition. (bio-medicine.org)
2.0 x10(11) platelets per unit or per local regulations) is prepared by pooling the buffy coat layer from up to six whole blood donations. (diva-portal.org)
A procedure for producing "double dose" whole blood derived platelets has only recently been developed. (diva-portal.org)
A buffy coat contains white blood cells and platelets. (ands.org.au)
red Blood cells, white Blood cells, plasma and platelets are made naturally by the body in the bone marrow. (prezi.com)
The buffy coat is made up of white blood cells and platelets. (prezi.com)
Layer of WBCs and platelets lies between top layer of plasma and bottom layer of RBCs when anticoagulant been added to blood specimen. (studystack.com)
A thin, light-colored layer of white blood cells and platelets that lies between a top layer of plasma and a bottom layer of red blood cells when an anticoagulant has been added to a blood specimen. (studystack.com)
This narrow, white band is composed of white blood cells ( WBCs or leukocytes ) and cell fragments called platelets ( thrombocytes ). (getbodysmart.com)
The present essay aims to present updates in hematology and immunology that help understand how, when, and why subclinical inflammation underlies alloimmunization and circumstances characteristic of red blood cells and - even more frequently - platelets that contribute inflammatory mediators. (frontiersin.org)
Unlike red blood cells and platelets, all white blood cells are nucleated and can be classified by their nuclei structure as mononuclear or polymorphonuclear cells. (genengnews.com)
Red Blood Cells Platelets Fresh Frozen Plasma (FFP) Cryoprecipitated Anti-hemophilic Factor Granulocytes. (slideserve.com)
Blood: a fluid connective tissue composed of Plasma Formed elements Erythrocytes (red blood cells, or RBCs) Leukocytes (white blood cells, or WBCs) Platelets. (slideserve.com)

This review overview the significance and storage condition of biospecimens including whole blood, red blood cells (RBC), buffy coat, plasma, serum, DNA and RNA that derived from blood in human biobanks. (nih.gov)
Using real-time quantitative methylation-specific PCR (RTQ-MSP), we quantified methylated p16INK4a sequences and determined the fractional concentrations of circulating tumor DNA in plasma, serum, and peripheral blood cells collected preoperatively, intraoperatively, and postoperatively from 49 patients with hepatocellular carcinoma (HCC). (aacrjournals.org)
In this investigation, we measured concentrations of tumor-derived methylated p16INK4a sequences in plasma, serum, and buffy coat collected from HCC patients before, during, and after surgical resection. (aacrjournals.org)
The devices are employed in novel apparatus of the invention, for the collection, separation and filtration of blood serum and blood. (google.com)
The devices are employed in novel apparatus of the invention, for the collection, separation and filtration of blood serum and blood plasma from clotted whole blood and anti-coagulant treated whole blood, respectively. (google.com)
The NucleoSpin 8/96 Blood QuickPure kits are designed for the rapid parallel isolation of genomic DNA from whole blood, serum, plasma, and body fluids (amniotic fluid, liquor, etc.)-in flexible 8-well strip or high-throughput 96-well plate formats which have been optimized for manual use. (clontech.com)

In order to identify a quick and reliable technique for accurate diagnosis of malaria, study of the efficiency of the tests such as Parahit total (HRPII & aldolase Ag), Advantage mal card (parasite specific LDH), and modified QBC was done in comparison with conventional blood smear microscopy. (hindawi.com)
The use of the Giemsa-stained thick blood smear for the diagnosis of malaria has not been supplanted since the discovery of the parasite by A. Laveran in 1880. (ajtmh.org)
We compared this technique with the thick blood smear diagnosis in a study of the efficacy of chloroquine therapy in Zaire. (ajtmh.org)
Chloroquine resistance was detected in 38% of the subjects by thick blood smear and in 45% by the QBC test. (ajtmh.org)
Of greater interest was the time required for each diagnosis: an average of 17 min was required to examine microscopic fields with 1,000 leukocytes by thick blood smear analysis compared with less than one min by the QBC system. (ajtmh.org)
Present study was undertaken to compare and study the efficacy of peripheral blood smear (PBS), modified centrifuged buffy coat smear (CBCS) and rapid malaria antigen detection test (RDT) in malarial diagnosis. (ijcmas.com)
The present study was designed to determine and compare the sensitivity and specificity of blood culture with Acridine Orange [A.O.] stained smear of buffy coat in patients with sepsis . (bvsalud.org)
A.O. stained smear of buffy coat, as a diagnostic test of sepsis is more sensitive than blood culture . (bvsalud.org)
Diagnosis is by finding the parasite in a blood smear or in the fluid of a lymph node. (wikipedia.org)
A hematology study which consists of a red cell count, white cell count, hematocrit, hemoglobin, and blood smear study including differential white cell count. (free-ed.net)
An en1.meration of the types of white blood cells seen on a stained blood smear. (free-ed.net)
Smear (buffy coat). (wardsci.com)
E. equi morulae were observed in a buffy coat smear stained with Giemsa. (asm.org)

Fractionation of white blood cells (WBCs), aka leukocytes, offers several advantages over use of whole blood, including concentration of the sample, removal of the RNases found in plasma, and the ability to use a more homogeneous population of cells for analysis. (thermofisher.com)
Blood fractionation to obtain the "buffy coat" fraction containing the WBCs involves centrifuging anticoagulated blood to separate the cell components according to density, allowing recovery of the total leukocyte population. (thermofisher.com)
The extent to which centrifugation affects mRNA profiles in WBCs recovered in the buffy coat fraction is a concern. (thermofisher.com)
1. Hematocrit Your answer : a. refers to the percentage of white blood cells (WBCs) in a sample of whole blood. (bartleby.com)
In this study, we examined the levels of the normal, cellular prion protein (PrPC), and the disease-causing isoform (PrPSc) in subpopulations of circulating white blood cells (WBCs) from patients with sporadic (s) CJD, age-matched neurological controls and healthy donors. (nih.gov)

red blood cells ( RBCs or erythrocytes ). (getbodysmart.com)
Red blood cells (RBCs) in normal human blood tend to form linear and branched aggregates. (spiedigitallibrary.org)
Correct answer: b. of 40 means that 40% of the volume of blood consists of RBCs. (bartleby.com)

Separating out this particular component enables the researcher to enrich the DNA-containing leukocytes and eliminate the heme-containing red blood cells. (bio-medicine.org)
This step is very important due to the presence of high numbers of white blood cells. (bio-medicine.org)
INTERCEPT was developed to inactivate viruses, bacteria, parasites, and contaminating donor white cells which may be present in donated blood. (diva-portal.org)
Whole cord blood DNA reflects all nucleated blood cell types, while centrifuging whole blood separates red blood cells, generating a white blood cell buffy coat. (elsevier.com)
There are about one billion red Blood cells in a few drops of whole Blood. (prezi.com)
Red Blood cells live about 120 days in our bodies. (prezi.com)
This is just red blood cells, which are the largest single component in blood. (prezi.com)
Altogether, these results showed that this novel storage method provides an adequate environment for ambient temperature long term storage of high molecular weight DNA in dehydrated lysates of white blood cells and buffy coats. (imagene.eu)
A dual-layer paper microfluidic chip was developed as a quicker, low-cost, and field-deployable alternative to detect ROR1+ (receptor tyrosine-like orphan receptor one) cancer cells from the undiluted and untreated buffy coat blood samples. (elsevier.com)
Our proposed device is able to capture and on-chip image ROR1+ cancer cells within a complex sample matrix (buffy coat) while simultaneously quantifying cell concentration in a point-of-care manner. (elsevier.com)
The EasySep™ HLA Chimerism Buffy Coat CD56 Positive Selection Kit II is designed to isolate CD56+ cells from freshly prepared buffy coat by positive selection. (stemcell.com)
Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD56 and dextran-coated magnetic particles. (stemcell.com)
Breakdown of blood cells. (studystack.com)
An increase in the concentration of the nonfilterable blood components in the blood vessels, such as red blood cells, enzymes, iron, and calcium, as a result of the decrease in the fluid content of the blood. (studystack.com)
contamination of the buffy coat fraction with red blood cells is unavoidable. (thermofisher.com)
Fractionation of Blood Cells by Centrifugation Minimally Affects mRNA Expression Levels. (thermofisher.com)
Isolation of DNA from whole blood can be difficult because blood is a complex mixture containing cells, proteins, metabolites, etc. (sigmaaldrich.com)
99%) are erythrocytes (red blood cells) which lack nuclei, and therefore, possess no DNA. (sigmaaldrich.com)
Leukocytes (white blood cells) contain nuclei and DNA. (sigmaaldrich.com)
The DNA Isolation Kit for Mammalian Blood procedure relies on the separation of white blood cells from whole blood via a preferential red blood cell lysis. (sigmaaldrich.com)
In the presence of a strong anionic detergent, the white blood cells are lysed, and then the proteins are removed by dehydration and precipitation. (sigmaaldrich.com)
The NucleoSpin 8/96 Blood kits are designed for the rapid parallel isolation of genomic DNA from whole blood or cultured cells. (clontech.com)
After lysis of blood cells, genomic DNA is selectively bound to a silica membrane, while contaminants are thoroughly washed away. (clontech.com)
A novel retrovirus, xenotropic murine leukemia virus-related virus (XMRV), has been detected in prostate cancer samples and in peripheral blood mononuclear cells (PBMC) from patients with chronic fatigue syndrome. (aacrjournals.org)
Much recent attention has focused on a paper by Lombardi and colleagues ( 6 ) that reported the detection of XMRV in peripheral blood mononuclear cells (PBMC) from 68 of 101 (67.3%) patients with chronic fatigue syndrome (CFS). (aacrjournals.org)
Hematocrit is the proportion of your total blood volume that contains red blood cells. (smore.com)
A hematocrit test is done using a sample of your blood to see if you have too few or too many red blood cells. (smore.com)
3) Technician can determine what proportion of cells are red blood cells. (smore.com)
Condition that occurs when your blood lacks healthy red blood cells or hemoglobin. (smore.com)
2) Hemolytic anemia- red blood cells are destroyed and removed from the blood stream before their normal life span is over. (smore.com)
4) Sickle cell anemia- body makes crescent shaped blood cells. (smore.com)
Polycythemia- Increased level of circulating blood cells in the bloodstream. (smore.com)
This buffy coat is then smeared on glass slides to search for LE cells. (medscape.com)
MPure Blood DNA Extraction Kit 200 is used with the MPure-12 aNAP System for extraction of DNA from 100-400 µL mammalian whole blood, suspension of mammalian blood cells. (mpbio.com)
Large sub inventory of antigen negative red blood cells Irradiation Services CMV negative. (ribc.org)
A fatal condition of the blood-forming tissues, characterized by marked proliferation of immature cells in the bone marrow, without their presence, in any great numbers, in the blood steam. (free-ed.net)
The ratio between the amount of hemoglobin and the number of red blood cells. (free-ed.net)
Target type red blood cells are often present in the peripheral blood. (free-ed.net)
Red blood cells normally occupy about 45% of the total blood volume ( females = 37-48% & males = 45-52% ). (getbodysmart.com)
Standing glass tube containing whole blood - red blood cells. (getbodysmart.com)
These enzyme tests can be carried out using blood, skin biopsy or saliva as the source of cells and care must be taken to use the most specific assays possible, especially for cases of later onset. (ucl.ac.uk)
Isolation of viable mononuclear and polymorphonuclear cells from blood serves as the starting point for a wide spectrum of immunology and cell biology studies. (genengnews.com)
Lymphocytes and monocytes are the two main categories of mononuclear white blood cells with one-lobed nuclei in the immune system to defend the body from infection, cancer, and other foreign invaders. (genengnews.com)
A pain point for many researchers is how to specifically isolate mononuclear and polymorphonuclear cells from blood with high yield and viability. (genengnews.com)
MP Biomedicals offers three products for the isolation of mononuclear and polymorphonuclear cells from human peripheral blood as well as from bone marrow and umbilical cord blood. (genengnews.com)
Isolation of mononuclear cells from whole blood using Lymphocyte Separation Medium (LSM). (genengnews.com)
It is widely known that cells from epithelial tumors, e.g., breast cancer, detach from their primary tissue and enter blood circulation. (mdpi.com)
Therefore, total RNA was isolated from blood samples of healthy donors and patients with primary or metastatic breast cancer after enrichment of mononuclear cells by density gradient centrifugation. (mdpi.com)
Leukapheresis was performed in each patient to isolate and characterise blood type 1 mDCs, and their functionality was studied in coculture with allogeneic CD4 + T cells from controls. (bmj.com)
Prion proteins in subpopulations of white blood cells from patients with sporadic Creutzfeldt-Jakob disease. (nih.gov)
Innocent white blood cells in sporadic Creutzfeldt-Jakob disease? (nih.gov)
Surface expression of PrP C on human peripheral white blood cells (WBC). (nih.gov)
The aim of this study was to assess the bioactivity of these compounds in Jurkat and K562 human leukemia cell models compared to peripheral blood mononuclear cells from healthy donors. (mdpi.com)

Buffy coat is the component of whole blood that contains leukocytes an. (bio-medicine.org)
Here we discuss an experiment that addresses whether blood cell fractionation affects the levels of several key mRNAs expressed in leukocytes. (thermofisher.com)
This strategy was used so that any differences in mRNA levels could not be attributed to using isolated fractionated leukocytes versus whole blood, but would instead relate to differences due to the experimental treatment. (thermofisher.com)
Therefore, DNA from blood must be isolated from one of three types of leukocytes: monocytes, lymphocytes, or granulocytes. (sigmaaldrich.com)
Isolated buffy coat: buffy coat from 10 to 20 mL whole blood, containing at least 1.0 x 107 leukocytes. (sigmaaldrich.com)
Complete or nearly complete absence of the granular leukocytes from the blood and bone marrow. (free-ed.net)
The layer of leukocytes that collects immediately above the erythrocytes in sedimented or centrifuged whole blood. (free-ed.net)
For many immunological and cell culture workflows, the first step is to specifically isolate leukocytes from the blood. (genengnews.com)
Early methods for isolating leukocytes involved mixing blood with a compound that aggregates the erythrocytes and, to a much lesser extent, the leukocytes. (genengnews.com)

It is characterized by the accumulation of CD5/CD19/CD23 positive B-lymphocytes in the blood, bone marrow, lymph nodes and spleen. (nature.com)
The DNA Isolation Kit for Mammalian Blood rapidly isolates DNA from 1 to 10 ml mammalian whole blood, lymphocytes, or buffy coat samples. (sigmaaldrich.com)
Lymphocytes should be isolated as a buffy coat and well fixed. (ucl.ac.uk)
Lymphocytes from patients with JNCL caused by mutations in CLN3 contain vacuolations that can be readily seen by light microscopy of a stained peripheral blood film, particularly in the trails. (ucl.ac.uk)
LSM allows for the separation of lymphocytes not only from human peripheral blood, but also from bone marrow as well as umbilical cord blood. (genengnews.com)
The separation principle used by LSM to isolate lymphocytes from the other blood constituents is summarized in Figure 1 . (genengnews.com)
2 Transmission typically follows exposure to infected lymphocytes in blood, or through breastfeeding or sexual intercourse. (mja.com.au)

Blood centers are faced with many challenges including maximizing production yield from the blood product donations they receive as well as ensuring the highest possible level of safety for transfusion patients, including protection from transfusion transmitted diseases. (diva-portal.org)
Implementation of buffy coat platelet component production: comparison to platelet-rich plasma platelet production," Transfusion , vol. 48, no. 11, pp. 2331-2337, 2008. (hindawi.com)
Comparison of blood component preparation methods from whole blood bags based on buffy coat extraction," Transfusion and Apheresis Science , vol. 36, no. 3, pp. 243-247, 2007. (hindawi.com)
Evaluation of overnight hold of whole blood at room temperature before component processing: effect of red blood cell (RBC) additive solutions on in vitro RBC measures," Transfusion , vol. 51, supplement 1, pp. 15S-24S, 2011. (hindawi.com)
In some countries, established supplies are limited and donors usually give blood when family or friends need a transfusion (directed donation). (wikipedia.org)
The screening includes testing for diseases that can be transmitted by a blood transfusion, including HIV and viral hepatitis . (wikipedia.org)
[2] An 'allogeneic' (also called 'homologous') donation is when a donor gives blood for storage at a blood bank for transfusion to an unknown recipient. (wikipedia.org)
A 'directed' donation is when a person, often a family member, donates blood for transfusion to a specific individual. (wikipedia.org)
[5] In this case, a friend or family member of the recipient donates blood to replace the stored blood used in a transfusion, ensuring a consistent supply. (wikipedia.org)
Blood transfusion is of huge importance in medicine, especially in emergency or life threatening situations. (hubpages.com)
The outstanding landmark in the history of blood transfusion is the discovery of human ABO blood group system in 1900 by Landsteiner. (hubpages.com)
This guidance for health professionals from Great Ormond Street Hospital (GOSH) is on manual exchange blood transfusion protocol. (gosh.nhs.uk)
In its earliest days, transfusion was clearly associated with acute inflammation, though the connection was not acknowledged as such: indeed, the very first reported serious adverse events (SAEs) of "modern" transfusion in the early twentieth century were dual in nature: first, immune-hematological [i.e., antigen-antibody (ABO)] conflicts, and second, blood-borne and blood-transmitted infections, such as syphilis and malaria ( 2 ). (frontiersin.org)
The safety of blood and the quantification of transfusion risks have been dominant themes that have stimulated the development of alternative approaches in this rapidly developing area. (springer.com)

The use of Gram type-specific PCR on buffy coat from clinical specimens for the detection of bacteraemia was evaluated for the first time using whole blood culture as the gold standard. (fiocruz.br)
These results demonstrated the clinical usefulness and effectiveness of peripheral blood RTQ-MSP for detecting and monitoring HCC after treatment. (aacrjournals.org)
However, cases with apparently normal enzyme levels in blood samples but clinical features consistent with disease caused by mutations in CLN1 / PPT1, CLN2 / TPP1 or CLN10 / CTSD should be followed up with enzyme and EM analysis of fibroblasts obtained via skin biopsy. (ucl.ac.uk)
In clinical medicine conventional blood and its components are used in supportive therapies dependent on the choice of apparent uncritical trigger factors. (springer.com)
A major obstacle to curing metastatic neuroblastoma is the presence of minimal residual disease (MRD) in the bone marrow and peripheral blood even after the patient has achieved clinical remission. (aacrjournals.org)
Blood samples from the remaining horses with clinical signs of EGE were collected during 1996 to 1998 in northern California. (asm.org)

The flow velocity was used as measure of antigen concentration in the buffy coat sample. (elsevier.com)
9. The method according to claim 8 , wherein the non-Y sequence is a blood group antigen gene. (google.ca)

Treatment: Blood transfusions, medications, surgery. (smore.com)
Treatment: Injections of albumin or plasma, transfusions of fresh, whole blood. (smore.com)
A blood donation occurs when a person voluntarily has blood drawn and used for transfusions and/or made into biopharmaceutical medications by a process called fractionation (separation of whole blood components). (wikipedia.org)
Most of the components of blood used for transfusions have a short shelf life , and maintaining a constant supply is a persistent problem. (wikipedia.org)
He researched in the field of blood transfusions , developing improved techniques for blood storage, and applied his expert knowledge to developing large-scale blood banks early in World War II . (wikipedia.org)
To avoid allogeneic transfusions, beneficial blood saving methods include various strategies, such as autologous deposits, normovolemic haemodilution and various agents including aprotinin, tranecamic acid, desmopressin and erythropoietin, but their use in hospital shows considerable variations. (springer.com)
Recent cases of prion transmission in humans following transfusions using blood donated by patients with asymptomatic variant Creutzfeldt-Jakob disease (CJD) implicate the presence of prion infectivity in peripheral blood. (nih.gov)

Short report: Detection of borrelia (relapsing fever) in rural Ethiopia by means of the quantitative buffy coat technique. (ajtmh.org)

The quantitative buffy coat (QBC) is a technique that is as sensitive as thick smears. (medscape.com)
Direct smears from cord blood were examined. (ashpublications.org)
A.O. stained smears of buffy coats were positive in 54 [54.5%] patients . (bvsalud.org)
Sensitivity and specificity of A.O. Stained smears of buffy coats were 83% and 100% respectively. (bvsalud.org)
citation needed] Some conventional parasitological techniques (CPT) such as wet blood film, and stained blood smears are used because so far, the best identifier is looking at the blood of the potentially infected host. (wikipedia.org)
In Burkina Faso, malaria treatment is prescribed after positive malaria rapid diagnostic test (RDT) or thick blood smears confirmation regardless of the nutritional status of the child. (academicjournals.org)

Several tubes of blood were collected from a healthy volunteer into evacuated tubes containing EDTA anticoagulant (Terumo). (thermofisher.com)
The ChargeSwitch ® gDNA Blood Kits are designed to allow isolation of genomic DNA from the following amounts of fresh or frozen, human blood treated with the anticoagulant EDTA or citrate. (thermofisher.com)

Temporary cessation of venous blood flow. (studystack.com)
The temporary cessation or slowing of the venous blood flow. (studystack.com)
therefore, the buffy coat from venous blood is an adequate substitute. (medscape.com)
If the equipment for buffy coat is unavailable, an untreated venous blood sample is left to clot (from 20-120 minutes) and the plasma removed. (medscape.com)
The time required for venous blood, in the absence of all tissue factors, to clot in glass tubes under controlled conditions. (free-ed.net)

DNA from 17 chimpanzees (lung, spleen, or liver), form a separate novel genus, with the proposed name of 30 red colobus monkeys (buffy coat, blood, bone marrow, Partetravirus, within the subfamily Parvovirinae . (cdc.gov)

Paired comparison of platelet concentrates prepared from platelet‐rich plasma and buffy coats. (deepdyve.com)

The versatile Perfect gDNA Blood Mini Kit allows the researcher to isolate gDNA from a variety of biological samples, including buffy coat. (bio-medicine.org)
ctx_ver=Z39.88-2004&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Adc&rfr_id=info%3Asid%2FANDS&rft_id=https://abctb.org.au/abctbNew2/researchspecimenquery.aspx&rft.title=BCTB Blood Buffy Coat Samples&rft.identifier=abctb.org.au 13&rft.publisher=Breast Cancer Tissue Bank&rft.description=This BCTB blood sample subcollection consists of buffy-coat samples. (ands.org.au)
This BCTB blood sample subcollection consists of buffy-coat samples. (ands.org.au)
No study has yet been made on the use of broad range Gram type-specific PCR on buffy coat samples for the rapid detection of bacteraemia. (fiocruz.br)
Blood cultures were taken and A.O. staining was done on blood samples of all patients . (bvsalud.org)
Preoperatively, the p16INK4a methylation indices ranged from 0.2 to 100% and from 0.012 to 0.075% in the patients' plasma and buffy coat samples, respectively. (aacrjournals.org)
Furthermore, none of the intraoperative plasma samples and only two of the intraoperative buffy coat samples were p16INK4a methylation positive. (aacrjournals.org)
With informed consent and ethics approval, 85 peripheral blood samples and 45 surgically removed tumors were collected prospectively from HCC patients. (aacrjournals.org)
There are numerous requests from basic scientists at UCSF for collaborative studies on specimens, especially for blood, saliva and urine samples. (clinicaltrials.gov)
Does Fractionation of Blood Samples Affect mRNA Expression Levels? (thermofisher.com)
Total RNA was extracted from all 6 samples (triplicate untreated and triplicate centrifuged samples) using the RiboPure™-Blood Kit, and 100 ng of each was used in qRT-PCR assays using Applied Biosystems Taqman® probes and primers. (thermofisher.com)
A patient's health may be diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more bodies or groups of bodies such as floats, inserts, liposomes, or plastic beads of different densities. (google.com.au)
Analyses of blood samples for the presence or absence of antibodies or antigens are used in the diagnosis of diseases, such as HIV infection, hepatitis, Lyme disease, prenatal profiles including TORCH (an acronym for: "Toxoplasmosis, Rubella, Cytomegalovirus, Herpes") profiles, as well as other infectious disease profiles. (google.com.au)
DNA was prepared from two research samples, one containing 10 ml human blood, the other containing 10 ml mouse blood. (sigmaaldrich.com)
Figure 2: Southern blot analysis of DNA from various human blood samples prepared with the DNA Isolation Kit for Mammalian Blood. (sigmaaldrich.com)
DNA was prepared from several research samples, including human blood containing different anticoagulants and lymphocyte and buffy coat preparations. (sigmaaldrich.com)
Animal or human blood samples and fresh or frozen material are processed conveniently via centrifugation of NucleoSpin Blood QuickPure Binding Strips/Plates. (clontech.com)
In contrast, subsequent studies from the United Kingdom, the Netherlands, China, and the United States have not detected XMRV in blood or PBMC samples from a total of more than 600 CFS cases examined ( 7-11 ). (aacrjournals.org)
Blood samples from patients with metastatic disease revealed increased cytokine gene levels in comparison to normal blood samples. (mdpi.com)
To measure erythrocyte count, arterial blood pH, ion concentrations, plasma glucose, and base deficit in the newborns during the first hour after birth, we collected arterial blood samples (1 mL) from the umbilical cord into heparin-containing tubes without the use of catheters. (thefreelibrary.com)
DNA extracted from tissue, buffy coat, and plasma samples was used for massively parallel DNA sequencing using the Ion Proton platform with a custom TargetSeq capture probe set covering all exons of 196 genes (4.1 Mb). (aacrjournals.org)
All tumor and buffy coat samples, and plasma samples from three patients have thus far been analyzed. (aacrjournals.org)
We achieved sequencing coverage of ∼100-fold for tumor and buffy coat DNA samples, and ∼1,000-fold for plasma DNA samples. (aacrjournals.org)
Quantitative reverse transcription-PCR was done on all 34 genes to study the dynamic range of tumor cell detection and the expression of these genes in normal marrow/blood samples and in stage 4 neuroblastoma tumors. (aacrjournals.org)
They were abundantly expressed in stage IV neuroblastoma tumors ( n = 20) and had low to no detection in normal marrow/blood samples ( n = 20). (aacrjournals.org)
The blood samples were tested for E. equi by 16S rRNA nested PCR ( 6 ), and the DNA samples were stored until use. (asm.org)

Solutions for oxygen transport are being developed and fibrinogen coated microcapsules are being investigated for thrombocytopenia. (springer.com)

What components of blood are separated from a centrifuge? (brainscape.com)
Most dense component 1 Centrifuge the blood sample. (slideserve.com)

Cord blood DNA methylation is associated with numerous health outcomes and environmental exposures. (elsevier.com)
The morphology of buffy coat from cord blood of normal human newborns was investigated and the results were compared with our previous observations in adults. (ashpublications.org)
The degree of hypoxia was ascertained by measurement of Hyp and Xan concentrations in cord blood. (thefreelibrary.com)

The diagnosis of louse-borne relapsing fever is commonly made on the basis of the detection of Borrelia spirochetes on Giemsa-stained thin blood films. (ajtmh.org)
Microscopical examination (ME) of stained blood films remains the gold standard method for the detection of these infections in birds, particularly because co-infections predominate in wildlife. (biomedcentral.com)
Unlike blood culture, nucleic acid-based assays do not require growth of an organism for detection, are proven sensitive in diagnosing nonculturable pathogens and have a shorter turnaround time (Greisen et al. (fiocruz.br)
Quantification of epigenetic changes in peripheral blood by RTQ-MSP is useful for the detection and monitoring of HCC. (aacrjournals.org)
Buffy coat can be used to increase detection. (wikipedia.org)
The invention enables non-invasive prenatal diagnosis including for example sex determination, blood typing and other genotyping, and detection of pre-eclampsia in the mother. (google.ca)

Recently, a new direct diagnosis technique, the Quantitative Buffy Coat (QBC®) * Malaria Diagnosis System, has been developed. (ajtmh.org)
What is the role of the quantitative buffy coat (QBC) technique in the diagnosis of malaria? (medscape.com)
The quantitative buffy coat [QBC] capillary tube was compared with both the thin and thick films in the diagnosis of malaria among 3 groups of hospitalized patients . (bvsalud.org)

Although separating the blood into its components requires an additional step, the benefits of higher gDNA concentration and yield out-weigh the extra effort. (bio-medicine.org)
Quality analysis of blood components obtained by automated buffy-coat layer removal with a top and bottom system," Haematologica , vol. 85, no. 4, pp. 390-395, 2000. (hindawi.com)
Increase in the concentration of the non-filterable blood components. (studystack.com)
How do you store different blood components and for how long? (brainscape.com)
The collection can be done manually or with automated equipment that takes only specific components of the blood. (wikipedia.org)
These programs allow our blood center to ship and/or receive blood components to maintain optimal blood supplies at all times. (ribc.org)
Approximately 1% of the blood volume is occupied by these components. (getbodysmart.com)

DNA Isolation Kit for Mammalian Blood has been used in isolation of genomic DNA from blood of asthmatic patients for single nucleotide polymorphisms genotyping. (sigmaaldrich.com)
The ChargeSwitch ® gDNA Purification Kits allow rapid and efficient purification of genomic DNA from small volumes of human blood. (thermofisher.com)
ChargeSwitch ® gDNA 20 µl Blood Kits: Purifies up to 600 ng of genomic DNA from 10-20 µl of human blood. (thermofisher.com)
ChargeSwitch ® gDNA 100 µl Blood Kit: Purifies up to 3 µg of genomic DNA from 50-100 µl of human blood. (thermofisher.com)

A general inventory of blood and cardiovascular-related tissues will be posted on the Cardiovascular Center's InfoScope webpage and/or is available via email ([email protected]) for investigators prior to application. (mcw.edu)
Other tissues can be looked at, but the gold standard is identifying trypanosomes in the blood. (wikipedia.org)

Nucleic acids extracted from MPure Blood DNA Extraction Kit 200 can be used in a number of downstream application including: PCR, qPCR, Sequencing (NGS), Microarray, RFLP, Southern Blot Analysis. (mpbio.com)
Please note that DNA is extracted on an 'as needed' basis so the date of extraction does not correlate with the date of the blood draw. (whi.org)

In the present study, we used acridine orange-coated quantitative buffy coat (QBC) tubes, centrifugation, and fluorescence microscopy to detect Borrelia. (ajtmh.org)

The versatile Perfect gDNA Blood Mini Kit allows the researcher to iso. (bio-medicine.org)

Blood of 345 birds belonging to 42 species was collected, and each sample was examined using ME of stained blood films and the buffy coat, which was examined after centrifugation in capillary tubes and after being transferred to objective glass slides. (biomedcentral.com)
This means a good indicator of disease would be looking at trypanosome density in capillary blood and connective tissue. (wikipedia.org)

The buffy coat is seen as a milky white layer between the clear upper plasma layer and the lower red blood cell layer. (bio-medicine.org)
3 genotypes of PARV4 have been found to infect humans and-white colobus monkeys (buffy coat, liver, intestine, ( 6 , 7 ). (cdc.gov)
There are 5 different types of white blood cell and each have a different function and role in protecting us. (prezi.com)
Carmen, R. 1992-02-01 00:00:00 Two techniques for the preparation of platelet concentrate (PC), the standard platelet‐rich plasma (PRP) and buffy coat (BC) methods, were compared in nine paired studies with regard to platelet harvest, white cell (WBC) contamination, and PC quality after 5 days of 22°C storage. (deepdyve.com)
Here we validated a novel procedure for ambient temperature preservation of DNA in white blood cell and buffy coat lysates based on the confinement of the desiccated biospecimens under anoxic and anhydrous atmosphere in original hermetic minicapsules. (imagene.eu)
Diagnosis of hematological cancer requires complete white blood cell count, followed by flow cytometry with multiple markers, and cytology. (elsevier.com)

These vials are usually glass and may hold various amounts of blood with 1.5 ml being the minimum amount required for this procedure. (bio-medicine.org)
The plasma was aspirated into a 5 ml pipet and then was returned to the sample (to mimic the procedure used to collect buffy coat). (thermofisher.com)
The NucleoSpin Blood QuickPure method features a specially treated silica membrane which speeds up the procedure by allowing a combined washing/drying step. (clontech.com)
Due to its plate design and the possibility of performing the complete procedure under vacuum, NucleoSpin 8/96 Blood can be easily adapted to common liquid handling instruments for fully automated processing without manual interactions. (clontech.com)

Buffy coat is stored frozen and DNA is extracted from stored buffy coat when it is requested for an ancillary study. (whi.org)

This direct transmission technique involves them cutting the skin with their mouths, then lapping up the blood along with connective tissue fluid. (wikipedia.org)

Universal primers targeting the 16S rRNA gene in bacteria have been used in the identification of a wide range of bacterial pathogens in blood, cerebrospinal fluid (CSF) and other normally sterile body fluids (Klausegger et al. (fiocruz.br)

Pipette 200 l of buffy coat for each sample to be processed into a fresh microcentrifuge tube. (bio-medicine.org)
This study modified, tested and described the buffy coat method (BCM) for quick diagnostics (~ 20 min/sample) of avian blood parasites. (biomedcentral.com)
small sample size - ease of sample collection - ease of shipping (regular mail) - stability and low cost storage - offers a source for study of exogenous or endogenous compounds other than DNA - genotyping generally requires 10 ng/genotype, and with current technology as little as 2.5 ng per SNP so that scores to hundreds of genotypes could be obtained from one blood spot. (cdc.gov)
First sample was positive after review prompted by the quantitative buffy coat result. (cdc.gov)
An example of a common sample manipulation is fractionation of blood. (thermofisher.com)
2. The paraphernalia of claim 1 wherein said insert has a plurality of spaced-apart bands of different binding materials coated thereon, each of said bands being specific to a first epitope or other binding site on a different target analyte so as to enable said insert to scavenge different target analytes from the sample. (google.com.au)

A health care professional trained to collect blood specimens. (studystack.com)
This study seeks to confidentially collect blood from HIV-positive individuals and HIV-negative controls to provide basic scientists with specimens for collaborative studies. (clinicaltrials.gov)
Tumor specimens from two (n=4) or three (n=4) tumor sites and blood were obtained with one month. (aacrjournals.org)

The term vial refers to the tubes typically used for collection of the blood. (bio-medicine.org)

After surgical resection, the median p16INK4a methylation indices in plasma and buffy coat concordantly decreased 12- and 15-fold, respectively. (aacrjournals.org)
Among nondiabetics, short and medium-term exposures to PM2.5 particulate matter were associated with higher fasting blood glucose levels, and part of this association was mediated by ICAM1 gene promoter methylation. (mdibl.org)

The panel of mRNAs amplified was chosen to include genes whose expression in previous studies was shown to be especially volatile in blood (e.g., p53 tumor suppressor gene, cytokine IL-8, and c-jun oncogene). (thermofisher.com)
isolated monocytes from the peripheral blood of axial spondyloarthritis patients and healthy controls to study differential tumor necrosis factor alpha-induced protein 3 (TNFAIP3) regulation in blood-derived macrophages. (genengnews.com)
Tumor mutations were identified by comparison to buffy coat DNA sequences. (aacrjournals.org)

It is viscous and is present as a thin milky layer above the lower red blood cell layer. (bio-medicine.org)
Buffy coat processing produced red cell (RBC) units with 50 percent of the WBC contamination of conventionally prepared units (9.8 ± 6.2 × 108/unit vs. 18.9 ± 7.1 × 108/unit). (deepdyve.com)
This product replaces the EasySep™ Human Buffy Coat CD56 Positive Selection Kit (Catalog #18085) and EasySep™ HLA Buffy Coat CD56 Positive Selection Kit (Catalog #18085HLA), for even faster cell isolations. (stemcell.com)
Starting with freshly prepared buffy coat, the CD56+ cell content of the isolated fraction typically ranges from 95.8 - 99.5% (gated on CD45+ using "The Big Easy" EasySep™ Magnet). (stemcell.com)
12. The method of claim 1 including passing a red blood cell-containing biological fluid through the leukocyte depletion porous medium. (google.com)
Blood cell production depends on the body's need for oxygen. (smore.com)
Causes: abnormalities in red blood cell production, sleep apnea, certain tumors, inherited genetic mutations. (smore.com)
This information from Great Ormond Street Hospital (GOSH) provides guidance for health professionals on cell salvage blood (intraoperative), administration in a non-theatre setting. (gosh.nhs.uk)
cessation of blood cell formation. (free-ed.net)
Red blood cell. (free-ed.net)
Light-transmission and light-backscattering methods are commonly used to determine red-blood-cell (RBC) aggregation. (spiedigitallibrary.org)
We report the identification of selective PI3Kδ inhibitors that blocked B-, T-, and plasmacytoid dendritic cell activities in human peripheral blood and in primary cell co-cultures (BioMAP ® ) without detecting signs of undesired toxicity. (frontiersin.org)

Repository of Human Blood Derivative Biospecimens in Biobank: Technical Implications. (nih.gov)

What is used to replace missing or low levels of proteins in blood? (brainscape.com)

He differentiated human blood into four distinct groups on the basis of two antigens A and B . (hubpages.com)
Alternative approaches include the use of cytokines, growth factors, humanised monoclonal antibodies, recombinant plasma factors, and buffy coat derived natural human interferons. (springer.com)

Separating blood allows to separate red blood from antibodies, so the recipient won't receive the donors antibodies as well. (brainscape.com)
Today in the developed world , most blood donors are unpaid volunteers who donate blood for a community supply. (wikipedia.org)
Many donors donate for several reasons, such as a form of charity, general awareness regarding the demand for blood, increased confidence in oneself, helping a personal friend or relative, and social pressure. (wikipedia.org)
Potential donors are evaluated for anything that might make their blood unsafe to use. (wikipedia.org)
With the generous support of volunteer blood donors and the community, the Rhode Island Blood Center has developed an industry leading blood collection program. (ribc.org)
To address this issue, we sorted DC precursors from the peripheral blood of CLL patients and found a profoundly altered compartment as compared with normal donors. (aacrjournals.org)

Type AB plasma has been considered as the universal Blood plasma type, and therefore AB plasma is given to patients with any Blood type. (prezi.com)
Blood cultures were positive in 20 [19.8%] patients . (bvsalud.org)
This study shows that blood mDCs from patients with occupational asthma and persistent disease despite allergen avoidance are imprinted with pro-Th2 activity. (bmj.com)
Eight different WBC fraction types from the peripheral blood of patients with sCJD were assessed for PrPSc. (nih.gov)

Figure 1: Amplification of large DNA fragments from mammalian blood prepared with the DNA Isolation Kit for Mammalian Blood. (sigmaaldrich.com)

A condition in which the blood is deficient in quantity or quality of erythrocytes. (free-ed.net)

Pairing INTERCEPT with the double dose buffy coat method by utilizing the INTERCEPT Processing Set with Dual Storage Containers (the "DS set"), allows blood centers to treat each of their double dose units in a single pathogen inactivation processing set, thereby maximizing patient safety while minimizing costs. (diva-portal.org)
The double dose buffy coat method requires fewer buffy coats and reduces the use of consumables by up to 50% (e.g. pooling sets, filter sets, platelet additive solution, and sterile connection wafers) compared to preparation and treatment of single dose buffy coat platelet units. (diva-portal.org)
From this point of view, ME of stained blood films can still be considered as the gold standard method for blood parasite biodiversity research in wildlife. (biomedcentral.com)
Blood culture would not be made obsolete by this method because pure isolates are required for antimicrobial drug susceptibility testing. (fiocruz.br)

Volumes ranging from 20 l to 200 l of buffy coat were taken from an entire vial (3 ml) of blood and tested for handling, yield, concentration, and purity as well as downstream applications such as PCR1. (bio-medicine.org)

Anatomy6

Organisms4

Diseases3

Chemicals and Drugs5

Analytical, Diagnostic and Therapeutic Techniques and Equipment8

Psychiatry and Psychology1

Phenomena and Processes8

Disciplines and Occupations2

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture1

Named Groups1

Health Care1

Transcription of Alu DNA elements in blood cells of sporadic Creutzfeldt-Jakob disease (sCJD). (1/25)

Methylprednisolone prevents tumour necrosis factor-alpha-dependent multinucleated giant cell formation. (2/25)

The Mirasol Pathogen Reduction Technology system and quality of platelets stored in platelet additive solution. (3/25)

Effects of storage time and leucocyte burden of packed and buffy-coat depleted red blood cell units on red cell storage lesion. (4/25)

Development and validation of a new, sensitive immunochemical assay for O(6)-methylguanine in DNA and its application in a population study. (5/25)

IL-4 and IL-13 alter plasmacytoid dendritic cell responsiveness to CpG DNA and herpes simplex virus-1. (6/25)

Merkel cell polyomavirus DNA sequences in the buffy coats of healthy blood donors. (7/25)

The histamine H4 receptor is highly expressed on plasmacytoid dendritic cells in psoriasis and histamine regulates their cytokine production and migration. (8/25)

Blood Buffy Coat

Psoralens

Platelet Transfusion

Blood Platelets

Plateletpheresis

Platelet Aggregation

Child, Exceptional

DNA Methylation

Methylation

Fetal Blood

National Institutes of Health (U.S.)

CpG Islands

Epigenesis, Genetic

Haemosporida

Protozoan Infections, Animal

Bird Diseases

Apicomplexa

Birds

Filarioidea

Microspheres

Retinal Artery

Hemolysis

Buffers

Erythrocytes

Cell Separation

Viscosity

Rheology

Elasticity

Centrifugation

Parasitology

Leukocytes

Sulfites

Spectrometry, Fluorescence

Ink

Fluorescent Dyes

Taq Polymerase

Polymerase Chain Reaction

Transcription of Alu DNA elements in blood cells of sporadic Creutzfeldt-Jakob disease (sCJD). (1/25)

Methylprednisolone prevents tumour necrosis factor-alpha-dependent multinucleated giant cell formation. (2/25)

The Mirasol Pathogen Reduction Technology system and quality of platelets stored in platelet additive solution. (3/25)

Effects of storage time and leucocyte burden of packed and buffy-coat depleted red blood cell units on red cell storage lesion. (4/25)

Development and validation of a new, sensitive immunochemical assay for O(6)-methylguanine in DNA and its application in a population study. (5/25)

IL-4 and IL-13 alter plasmacytoid dendritic cell responsiveness to CpG DNA and herpes simplex virus-1. (6/25)

Merkel cell polyomavirus DNA sequences in the buffy coats of healthy blood donors. (7/25)

The histamine H4 receptor is highly expressed on plasmacytoid dendritic cells in psoriasis and histamine regulates their cytokine production and migration. (8/25)

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Pathogen Reduction Evaluation & Predictive Analytical Rating Score - Full Text View - ClinicalTrials.gov

Health News Med: FDA approves pathogen reduction system to treat platelets

Pathogen reduction through additive-free short-wave UV light irradiation retains the optimal efficacy of human platelet lysate...

Transfusion Interrégionale CRS :: Organization

Mirasol-Treated Platelets - (Pathogen Reduction Extended Storage Study) - Full Text View - ClinicalTrials.gov

Strategies for Improving or Creating Emission Reduction Technology and Byproduct Utilization for Hazardous Substances Resulting...

Edward Leonard Snyder, MD > Laboratory Medicine | Yale School of...

Platelet - Wikipedia

Platelet - Wikipedia

Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System

StraightFrom® Buffy Coat CD8 MicroBead Kit, human - StraightFrom MicroBeads - Cell separation reagents - MACS Cell Separation -...

StraightFrom® Buffy Coat CD4 MicroBead Kit, human - StraightFrom MicroBeads - Cell separation reagents - MACS Cell Separation -...

STATISTICAL FILTERING OF DATA USING GRAPHICAL USER INTERFACES IN INFORMATION TECHNOLOGY SYSTEMS - Patent application

Genetic Programming for Automating the Development of Data Management Algorithms in Information Technology Systems - pdf...

IMPACT Silver Discovers Wide Silver Veins in First Drill Holes at Mirasol in Zacualpan, Mexico; Intersections Include 7.1...

octobre | 2014 | Macopharma

Maxwell® RSC Buffy Coat DNA Kit

Certification & Testing Approvals

Human Cytomegalovirus and Human Umbilical Vein Endothelial Cells: Restriction of Primary Isolation to Blood Samples and...

HTQC | A quality control toolkit for Illumina sequencing data - OMICtools

Clinical Trials - Infectious Diseases in Florida - Mayo Clinic

Broad spectrum pathogen reduction

Why pathogen reduction?

Transfusion publishes scientific paper regarding viral safety of plasma-derived therapeutics - Plasma Protein Therapeutics...

Petroleum Reduction Technologies:Hydrogen | naftc ?...

Research, University of Cincinnati