A genus of protozoa of the suborder BLASTOCYSTINA. It was first classified as a yeast but further studies have shown it to be a protozoan.
Infections of the INTESTINES with PARASITES, commonly involving PARASITIC WORMS. Infections with roundworms (NEMATODE INFECTIONS) and tapeworms (CESTODE INFECTIONS) are also known as HELMINTHIASIS.
Infections with unicellular organisms formerly members of the subkingdom Protozoa.
Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
A country in northern Africa, bordering the Mediterranean Sea, between Egypt, Tunisia, and Algeria, having southern border with Chad, Niger, and Sudan. Its capital is Tripoli.
The study of parasites and PARASITIC DISEASES.
Deoxyribonucleic acid that makes up the genetic material of protozoa.
Enzymes that catalyze the first step leading to the oxidation of succinic acid by the reversible formation of succinyl-CoA from succinate and CoA with the concomitant cleavage of ATP to ADP (EC 6.2.1.5) or GTP to GDP (EC 6.2.1.4) and orthophosphate. Itaconate can act instead of succinate and ITP instead of GTP.EC 6.2.1.-.
A common name (but used formally) for a group of organisms that are mostly kinds of algae including BACILLARIOPHYTA; OOMYCETES; PHAEOPHYCEAE; and CHRYSOPHYCEAE. They all contain CHLOROPLASTS that are thought to have been derived from the endosymbiosis of ancient RED ALGAE.
A nitroimidazole used to treat AMEBIASIS; VAGINITIS; TRICHOMONAS INFECTIONS; GIARDIASIS; ANAEROBIC BACTERIA; and TREPONEMAL INFECTIONS. It has also been proposed as a radiation sensitizer for hypoxic cells. According to the Fourth Annual Report on Carcinogens (NTP 85-002, 1985, p133), this substance may reasonably be anticipated to be a carcinogen (Merck, 11th ed).

Blastocystis isolates from a pig and a horse are closely related to Blastocystis hominis. (1/51)

Blastocystis has a widespread distribution in a variety of animals, which is a potential source of infection for humans. However, the contribution of zoonotic transmission remains unclear due to the absence of molecular proof of these organisms being identical to those found in humans. We report herein the similar subgroup of Blastocystis isolates from humans, pigs, and a horse using a restriction fragment length polymorphism (RFLP) analysis of partial small-subunit ribosomal DNA (ssu rDNA). Additionally, sequence and phylogenic analysis of partial ssu rDNA of Blastocystis from a human, a pig, and a horse sharing a common subgroup shows that Blastocystis isolates from a pig and a horse were monophyletic and closely related to B. hominis, with 92 to 94% identity. These results suggest the possibility of zoonotic potential of Blastocystis.  (+info)

PCR-based identification of zoonotic isolates of Blastocystis from mammals and birds. (2/51)

The genotype of Blastocystis isolated from humans and animals is highly polymorphic. Therefore, it is important to compare the genotypes of Blastocystis isolates from humans and animals to determine the zoonotic potential of animal isolates. PCR-based genotype classification using known sequence-tagged site (STS) primers allows identification of zoonotic isolates of animal origin. To this end, 51 isolates from monkeys, cattle, pigs, chickens, quails and pheasants were subjected to genotype analysis using seven kinds of STS primers. Out of the 51 isolates, 39 were identified as one of the known genotypes, four showed mixed genotypes, and eight were unknown genotypes as these were negative for all STS primers. When these results were combined with previous studies on 41 isolates from animals and compared with the diversity of genotypes of 102 human Blastocystis hominis isolates, 67.4 % (62/92) of isolates from mammals and birds were identical to human B. hominis genotypes. Since the unknown genotype of human origin had been placed into an additional clade in the small-subunit rRNA gene phylogeny, further molecular study on the eight isolates of unknown genotype from the present study will facilitate our understanding of their zoonotic potential.  (+info)

Molecular phylogenies of Blastocystis isolates from different hosts: implications for genetic diversity, identification of species, and zoonosis. (3/51)

Small-subunit (SSU) rRNA gene sequences were obtained by PCR from 12 Blastocystis isolates from humans, rats, and reptiles for which elongation factor 1alpha (EF-1alpha) gene sequences are already available. These new sequences were analyzed by the Bayesian method in a broad phylogeny including, for the first time, all Blastocystis sequences available in the databases. Phylogenetic trees identified seven well-resolved groups plus several discrete lineages that could represent newly defined clades. Comparative analysis of SSU rRNA- and EF-1alpha-based trees obtained by maximum-likelihood methods from a restricted sampling (13 isolates) revealed overall agreement between the two phylogenies. In spite of their morphological similarity, sequence divergence among Blastocystis isolates reflected considerable genetic diversity that could be correlated with the existence of potentially >/=12 different species within the genus. Based on this analysis and previous PCR-based genotype classification data, six of these major groups might consist of Blastocystis isolates from both humans and other animal hosts, confirming the low host specificity of Blastocystis. Our results also strongly suggest the existence of numerous zoonotic isolates with frequent animal-to-human and human-to-animal transmissions and of a large potential reservoir in animals for infections in humans.  (+info)

Viable blastocystis cysts in Scottish and Malaysian sewage samples. (4/51)

Blastocystis cysts were detected in 38% (47/123) (37 Scottish, 17 Malaysian) of sewage treatment works. Fifty percent of influents (29% Scottish, 76% Malaysian) and 28% of effluents (9% Scottish, 60% Malaysian) contained viable cysts. Viable cysts, discharged in effluent, provide further evidence for the potential for waterborne transmission of Blastocystis.  (+info)

Blastocystis ratti induces contact-independent apoptosis, F-actin rearrangement, and barrier function disruption in IEC-6 cells. (5/51)

Blastocystis is an enteric protozoan purportedly associated with numerous clinical cases of diarrhea, flatulence, vomiting, and other gastrointestinal symptoms. Despite new knowledge of Blastocystis cell biology, genetic diversity, and epidemiology, its pathogenic potential remains controversial. Numerous clinical and epidemiological studies either implicate or exonerate the parasite as a cause of intestinal disease. Therefore, the aim of this study was to investigate the pathogenic potential of Blastocystis by studying the interactions of Blastocystis ratti WR1, an isolate of zoonotic potential, with a nontransformed rat intestinal epithelial cell line, IEC-6. Here, we report that B. ratti WR1 induces apoptosis in IEC-6 cells in a contact-independent manner. Furthermore, we found that B. ratti WR1 rearranges F-actin distribution, decreases transepithelial resistance, and increases epithelial permeability in IEC-6 cell monolayers. In addition, we found that the effects of B. ratti on transepithelial electrical resistance and epithelial permeability were significantly abrogated by treatment with metronidazole, an antiprotozoal drug. Our results suggest for the first time that Blastocystis-induced apoptosis in host cells and altered epithelial barrier function might play an important role in the pathogenesis of Blastocystis infections and that metronidazole has therapeutic potential in alleviating symptoms associated with Blastocystis.  (+info)

Cellular identification of a novel uncultured marine stramenopile (MAST-12 Clade) small-subunit rRNA gene sequence from a norwegian estuary by use of fluorescence in situ hybridization-scanning electron microscopy. (6/51)

Revealing the cellular identity of organisms behind environmental eukaryote rRNA gene sequences is a major objective in microbial diversity research. We sampled an estuarine oxygen-depleted microbial mat in southwestern Norway and retrieved an 18S rRNA gene signature that branches in the MAST-12 clade, an environmental marine stramenopile clade. Detailed phylogenetic analyses revealed that MAST-12 branches among the heterotrophic stramenopiles as a sister of the free-living Bicosoecida and the parasitic genus Blastocystis. Specific sequence signatures confirmed a relationship to these two groups while excluding direct assignment. We designed a specific oligonucleotide probe for the target sequence and detected the corresponding organism in incubation samples using fluorescence in situ hybridization (FISH). Using the combined FISH-scanning electron microscopy approach (T. Stoeck, W. H. Fowle, and S. S. Epstein, Appl. Environ. Microbiol. 69:6856-6863, 2003), we determined the morphotype of the target organism among the very diverse possible morphologies of the heterotrophic stramenopiles. The unpigmented cell is spherical and about 5 mum in diameter and possesses a short flagellum and a long flagellum, both emanating anteriorly. The long flagellum bears mastigonemes in a characteristic arrangement, and its length (30 mum) distinguishes the target organism from other recognized heterotrophic stramenopiles. The short flagellum is naked and often directed posteriorly. The organism possesses neither a lorica nor a stalk. The morphological characteristics that we discovered should help isolate a representative of a novel stramenopile group, possibly at a high taxonomic level, in order to study its ultrastructure, physiological capabilities, and ecological role in the environment.  (+info)

Blastocystis ratti contains cysteine proteases that mediate interleukin-8 response from human intestinal epithelial cells in an NF-kappaB-dependent manner. (7/51)

Blastocystis is a ubiquitous enteric protozoan found in the intestinal tracts of humans and a wide range of animals. Evidence accumulated over the last decade suggests association of Blastocystis with gastrointestinal disorders involving diarrhea, abdominal pain, constipation, nausea, and fatigue. Clinical and experimental studies have associated Blastocystis with intestinal inflammation, and it has been shown that Blastocystis has potential to modulate the host immune response. Blastocystis is also reported to be an opportunistic pathogen in immunosuppressed patients, especially those suffering from AIDS. However, nothing is known about the parasitic virulence factors and early events following host-parasite interactions. In the present study, we investigated the molecular mechanism by which Blastocystis activates interleukin-8 (IL-8) gene expression in human colonic epithelial T84 cells. We demonstrate for the first time that cysteine proteases of Blastocystis ratti WR1, a zoonotic isolate, can activate IL-8 gene expression in human colonic epithelial cells. Furthermore, we show that NF-kappaB activation is involved in the production of IL-8. In addition, our findings show that treatment with the antiprotozoal drug metronidazole can avert IL-8 production induced by B. ratti WR1. We also show for the first time that the central vacuole of Blastocystis may function as a reservoir for cysteine proteases. Our findings will contribute to an understanding of the pathobiology of a poorly studied parasite whose public health importance is increasingly recognized.  (+info)

Organelles in Blastocystis that blur the distinction between mitochondria and hydrogenosomes. (8/51)

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To determine the distribution of Blastocystis sp. subtypes from Blastocystis cyst excreters, 1,000 fecal samples from patients suspected of enteroparasitic disease were scored for stool consistency, submitted to xenic in vitro culture (XIVC), formol ethyl acetate concentration (FECT) with subsequent isopycnic centrifugation, and polymerase chain reaction (PCR) with subtype (ST) analysis. Blastocystis was significantly more prevalent in specimens from patients with travel-associated diarrhea (15.6%) than those with persistent diarrhea (8.3%) (P = 0.005). Overall, 115 (11.5%) and 35 (3.5%) specimens were positive by XIVC and FECT, respectively. Blastocystis cysts were detected in 33 (28.7%) of the XIVC-positive specimens. A positive FECT result was associated with ST3 (P = 0.05). The presence of Blastocystis in general or Blastocystis cysts was independent of stool consistency, and no particular ST was significantly associated with cyst identification. In view of these data, the present study indicates
We determined cytokines (e.g. interleukin-8, 10, 12 and TNF-α) expression by peripheral blood mononuclear cells (PBMCs) and in rectal mucosa in diarrhoea-predominant irritable bowel syndrome (D-IBS) with Blastocystis spp. Eighty patients with D-IBS and Blastocystis spp. infection were classified as cases and 80 with D-IBS without Blastocystis spp. infection were classified as control. Cases were subdivided into D-IBS and Blastocystis sp. defined type 1 (subtype-specific primer SB83) and type 3 (SB227). Stool microscopy and culture were performed. Rectal biopsies were obtained for histology and cytokines by real-time PCR for mRNA expression of cytokines. PBMCs IL-8 was similar in different groups but in type 1, IL-8mRNA was increased compared with type 3 (P = 0·001) and control (P = 0·001). In type 1, IL-10 by PBMCs had a low mean value (14·5±1·6) compared with (16·7±1·5) type 3 and (16±2·3) in controls (P
Abstract This study aimed to elucidate aspects of the epidemiology of Blastocystis in Nigerian school children, including the distribution of subtypes (STs) and ST alleles. A total of 199 genomic DNAs extracted from fecal samples from 199 Nigerian children aged 2-14 years were tested by real-time polymerase chain reaction for Blastocystis. Positive DNAs were submitted to barcoding by PCR and sequencing to obtain information on STs and ST alleles. A total of 167 (84%) samples were positive for Blastocystis, with prevalence increasing by age. No association between Blastocystis colonization and gender (P = 0.51) or type/presence of toilet facilities (P = 0.21) was observed. Blastocystis carriers were more prone to using water collected from wells than from sachets (P = 0.0044). Moreover, Blastocystis positivity was associated with positivity for fecal-orally transmitted protozoa (P = 0.018) and helminths (P < 0.0001). A clear inverse association of Blastocystis colonization and malaria infection was
Wawrzyniak, Ivan, Courtine, Damien, Osman, Marwan S., Hubans-Pierlot, Christine, Cian, Amandine, Nourrisson, Celine, Chabe, Magali, Poirier, Philippe, Bart, Aldert, Polonais, Valerie, Delgado-Viscogliosi, Pilar, El Alaoui, Hicham, Belkorchia, Abdel, van Gool, Tom, Tan, Shyong Wei Kevin, Ferreira, Stephanie, Viscoglios, Eric, Delbac, Frederic. (2015). Draft genome sequence of the intestinal parasite Blastocystis subtype 4-isolate WR1. Genomics Data 4 : 22-23. [email protected] Repository. https://doi.org/10.1016/j.gdata.2015.01. ...
Although Blastocystis is one of the most common enteric parasites, there is still much controversy surrounding the pathogenicity and potential treatment options for this parasite. In this review we look at the evidence supporting Blastocystis as an intestinal pathogen as shown by numerous case studies and several in vivo studies and the evidence against. We describe the chronic nature of some infections and show the role of Blastocystis in immunocompromised patients and the relationship between irritable bowel syndrome and Blastocystis infection. There have been several studies that have suggested that pathogenicity may be subtype related. Metronidazole is the most widely accepted treatment for Blastocystis but several cases of treatment failure and resistance have been described. Other treatment options which have been suggested include paromomycin and trimethroprim- sulfamethoxazole.
Blastocystis hominis is the most common intestinal protozoan infecting humans in the United States, but it is probably the least understood. The parasite is also the most frequently diagnosed intestinal protozoan in Oregon. Records from the Good Samaritan Regional Medical Center, Corvallis, diagnostic laboratory show 40 cases of Blastocystis in 2006 vs. 10 for Giardia lamblia, a usual cause of gastrointestinal symptoms. Indeed, the prevalence of the infection might be much higher as routine methods used to detect other intestinal protozoans often miss Blastocystis.1 2 Blastocystis has been a medical mystery for years. Many people who carry the organism show no symptoms, while others inexplicably become very ill. Recent research has identified multiple genetically diverse sub-types of Blastocystis which can infect humans,3 and suggested only a specific sub-group is responsible for the disease.4 5 This model would be consistent with research suggesting a phylogenetic link between Blastocystis and
Blastocystosis refers to a medical condition caused by infection with Blastocystis. Blastocystis is a protozoal, single-celled parasite that inhabits the gastrointestinal tracts of humans and other animals. Many different types of Blastocystis exist, and they can infect humans, farm animals, birds, rodents, amphibians, reptiles, fish, and even cockroaches. Blastocystosis has been found to be a possible risk factor for development of IBS (Irritable Bowel Syndrome). There are conflicting reports regarding whether Blastocystis causes disease in humans. These reports resulted in a brief debate in medical journals in the early 1990s between some physicians in the United States who believed that Blastocystis was harmless, and physicians in the United States and overseas who believed it could cause disease. At the time, it was common practice to identify all Blastocystis from humans as Blastocystis hominis, while Blastocystis from animals was identified differently (e.g. Blastocystis ratti from rats). ...
Blastocystis is one of the most common intestinal protozoa in human faecal samples with uncertain impact on public health. Studies on the prevalence of Blastocystis in HIV-positive patients are limited and dated. A cross-sectional study was carried out involving 156 HIV-positive patients to evaluate the prevalence of Blastocystis-subtypes by molecular amplification and sequencing the small subunit rRNA gene (SSU rDNA), to identify the risk factors for its transmission, to examine the relationship between the presence of the protist and gastrointestinal disorders. Furthermore, the evaluation of the faecal calprotectin by immunoassay from a sample of subjects was performed to evaluate the gut inflammation in Blastocystis-carriers. Blastocystis-subtypes ST1, ST2, ST3, ST4 were identified in 39 HIV-positive patients (25%). No correlation was found between the presence of the protist and virological or epidemiological risk factors. Blastocystis was more frequently detected in homosexual subjects (p = 0.037)
A mitochondrion-like organelle (MLO) was isolated from isotonic homogenates of Blastocystis. The organelle sedimented at 5000 g for 10 min, and had an isopycnic density in sucrose of 1.2 g ml−1. Biochemical characterization enabled the demonstration of several key enzymes that allowed the construction of a metabolic pathway consisting of an incomplete Krebs cycle linked to the oxygen-sensitive enzymes pyruvate : NADP+ oxidoreductase (PNO), acetate : succinate CoA transferase (ASCT) and succinate thiokinase (STK), which cumulatively are responsible for recycling CoA and generating ATP. The organelle differs from typical aerobic mitochondria in possessing an oxygen-sensitive PNO that can use FAD+ or FMN+ as electron acceptor but is inactive with NAD+, Spinacia oleracea ferredoxin or Clostridium pasteurianum ferredoxin. A gene with 77 % sequence similarity to the PNO mitochondrion precursor cluster from Euglena gracilis sp[Q941N5] was identified in the Blastocystis genome database. A second cluster with
Introduction: A lack of prospective and longitudinal data on pre- and post-travel carriage of Blastocystis spp. complicates interpretation of a positive test post-travel. Therefore we studied dynamics of Blastocystis carriage in a cohort of Dutch travellers. Methods: From the prospective, multicentre COMBAT study among 2001 Dutch travellers, a subset of 491 travellers was selected based on travel destination to 7 subregions (70 or 71 travellers each). Faecal samples taken directly before and after travel were screened for Blastocystis with qPCR, followed, when positive, by sequence analysis to determine subtypes. Results: After exclusion of 12 samples with missing samples or inhibited qPCR-reactions, stool samples of 479 travellers were analysed. Before travel, 174 of them (36.3%) carried Blastocystis and in most of these, the same subtype was persistently carried. However, in 48/174 of those travellers (27.6%; CI95 20.8-36.6%) no Blastocystis or a different subtype was detected in the ...
A recently described cytotoxic monoclonal antibody (mAb 1D5) raised against Blastocystis hominis isolate B, was tested for reactivity with 13 different isolates of Blastocystis. The isolates used were previously isolated from humans, rats and reptiles and were maintained as axenised cultures throughout the course of this stady. Five B. hominis isolates (B, C, E, G and H) were found to react with mAb 1D5 in immunoblotting studies and the indirect fluorescence antibody test. The pattern of fluorescence observed for all five isolates was diffuse and patchy. Immunoblotting studies revealed that mAb 1D5 reacted with a 29-30-kDa protein found in all five isolates. Results of a cytotoxic assay showed that the mAb exhibited a complement-independent cytotoxic effect on all the exposed isolates. Microscopic observations showed differences in morphology between the Blastocystis cells exposed and unexposed to mAb. Acridine orange staining performed on both exposed and unexposed cells showed similar internal ...
Blastocystis is a protist parasitising the intestine of humans and a variety of animals. We estimate that at least 1 billion people worldwide are colonised by this parasite, most of whom probably experience no more episodes of intestinal upset, e.g. diarrhoea, than the average individual. In any case, many people have Blastocystis wihtout knowing and without feeling sick. Blastocystis may colonise the intestine for a long time (i.e. months or years ...
Infections with organisms of the genus BLASTOCYSTIS. The species B. hominis is responsible for most infections. Parasitologic surveys have generally found small numbers of this species in human stools, but higher positivity rates and organism numbers in AIDS patients and other immunosuppressed patients (IMMUNOCOMPROMISED HOST). Symptoms include ABDOMINAL PAIN; DIARRHEA; CONSTIPATION; VOMITING; and FATIGUE ...
Going through the list of Microbiology Blogs we have curated at microBEnet and going to try to feature one of them every day or so. And just going to do this in semi-alphabetic order. Todays blog: Blastocystis Blog Author: Christen Rune Stensvold Tagline/Summary: Blastocystis may be found in more than 1 billion people worldwide. We investigate the role of …. ...
Hulda Clark suggested that all autoimmune diseases were actually caused by fluke parasites and pollutants. yes she has some good ideas, but I think way to simplified...she blames way to much on flukes, very few people in west have over flukes I think...one can test for several of them, and many people tried fluke medicines without getting better I think she has overlooked things like blastocystis hominis,and also her knowledge about diets seems limited... also her idea that basically all infections can be cured by zappers seems to be just an idea with little evidence...yes I wou ...
Hello, to anyone who is looking for a parasite treatment for Blastocystis Hominis, try looking up the website badbugs org, or the website for the Centre for Digestive Diseases in Sydney Australia. A ne...
Blastocystis Is Associated with Decrease of Fecal Microbiota Protective Bacteria: Comparative Analysis between Patients with Irritable Bowel Syndrome and Control Subjects. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Blastocystis is a unicellular, anaerobic protist which lives in the intestinal tract of diverse animals, including humans. It was found that the host specificity and the pathogenic potential of different isolates are correlated with sequence variations in the SSU-rRNA gene. Identification of...
mark rosal ,rosal at mail.netshop.net, writes: ,Hello to everyone, I have rather peristent case of blasocystis hominis, ,and the regular medicines- such a flagy dont seem to be able to get rid ,of it. Can any body help me on this one. I am willing to look at any ,thing if it can specifically knock this bug out. Any and all ,information, suggestions, contacts would be greatly appreciated. Thanks ,--- mark rosal Blastocystis infection is probably the most common parasitic = infection in the world. Some surveys indicate infection rates of 10% in Europe and North America and up to 50% in developing countries. Relatively little is known about Blastocystis hominis compared to = most parasites and not everyone agrees on all points. Approximately = equal numbers of doctors think that it causes disease as those who = do not believe it does. The reason for this is quite simply that = most people infected show no symptoms at all that can be linked to = the presence of the parasite. In any event, the most ...
Jundishapur Journal of Microbiology Official Publication of School of Medicine, Ahvaz Jundishapur University of Medical Sciences ...
Hello, I am new to this forum and the world of parasites and Candida. Not too long ago I was a successful business person with an active family life and social life. I was busy and now I am fighting to survive everyday. MY STORY I had been losing weight and dealing with increasing food allergies and decreasing energy. I was probably able to live life at 65% of the energy of my normal self. I did a homeopathic detox and it through me over the edge and nearly killed me. The Homeopath said that the drops were mild enough for a baby, but for some reason I was extremely sensitive to th ...
Omar O. Barriga (oobarrig at pop.service.ohio-state.edu) wrote: ,Blastocystis is not a pathogenic organism. This is far from being a settled issue. I would estimate that there are about an equal numbers of paper in the literature that say it is a pathogen as papers that say it is not. It may not cause invasive or fatal disease but that does not mean it is a commensal. It is also clear from the recent literature that human Blastocystis is actually two or more distinct organisms - this may in part explain the variable results being reported with respect to its role as a pathogen. Graham ____________________ C. Graham Clark, Ph.D. Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, England, G.B. Tel: ++44-171-927-2351 FAX: ++44-171-636-8739 e-mail: g.clark at lshtm.ac.uk ...
By Michael Biamonte, C.C.N. Several years ago, the Centers for Disease Control in Atlanta, Georgia along with some other experts and authorities, re-classified an organism known as Blastocystis Hominis-a common organism found in the intestinal tract of most people-as a dangerous disease-producing organism, as opposed to just a harmless organism. … ...
Skip salsa and other condiments made with fresh ingredients. Within regions inthesamecountry,theprevalencecanvarywidely. Accessed Dec. 8, 2018. It is still very much up in the air. Not the most straightforward answer but the gut is complex! Mayo, Mayo Clinic, MayoClinic.org, Mayo Clinic Healthy Living, and the triple-shield Mayo Clinic logo are trademarks of Mayo Foundation for Medical Education and Research. Hi Todd this was very interesting to read. It can be found in healthy people who arent having digestive symptoms, and its also sometimes found in the stools of people who have diarrhea, abdominal pain or other gastrointestinal problems.Researchers dont fully understand the role Blastocystis hominis plays, if any, in causing disease. Im at a loss as to what to do from here. Signs and symptoms possibly associated with Blastocystis hominis include: See your doctor if you have signs and symptoms, such as diarrhea or cramps, that last longer than three days. Happy it could help. Any ...
Tan, T.C.; Ong, S.C.; Suresh, K.G. (2009) Genetic variability of Blastocystis sp isolates obtained from cancer and HIV/AIDS patients. Parasitology Research, 105 (5). pp. 1283-1286. ISSN 0932-0113. Tan, T.C.; Suresh, K.G. (2006) Amoeboid form of Blastocystis hominis - a detailed ultrastructural insight. Parasitology Research, 99 (6). pp. 737-742. ISSN 0932-0113. Tan, T.C.; Suresh, K.G. (2007) Evidence of plasmotomy in Blastocystis hominis. Parasitology Research, 101 (6). pp. 1521-1525. ISSN 0932-0113. Tan, T.C.; Suresh, K.G. (2006) Predominance of amoeboid forms of Blastocystis hominis in isolates from symptomatic patients. Parasitology Research, 98 (3). pp. 189-193. ISSN 0932-0113. Tan, T.C.; Suresh, K.G.; Smith, H.V. (2008) Phenotypic and genotypic characterisation of Blastocystis hominis isolates implicates subtype 3 as a subtype with pathogenic potential. Parasitology Research, 104 (1). pp. 85-93. ISSN 0932-0113. Tan, T.C.; Suresh, K.G.; Thong, K.L.; Smith, H.V. (2006) PCR fingerprinting of ...
Below are some of the more common parasitic infections:. Blastocystis hominis. Although some consider Blastocystis hominis to be a commensal parasite, the more recent research shows that this is a pathogenic parasite. In addition, the research shows that Blastocystis hominis can cause an increase in inflammation (1), and even increase the permeability of the small intestines (2). A case report involving a 49-year old man with Hashimotos Thyroiditis shows that eradicating Blastocystis hominis resulted in a decrease of his thyroid antibodies (3). I personally have had a few patients with Hashimotos test positive for Blastocystis hominis and go into remission upon eradicating it.. Entamoeba histolytica. This is a pathogenic parasite that is associated with intestinal and extraintestinal infections (4). In other words, it not only can affect the intestines, but it can also affect extraintestinal sites such as the liver, brain, and lungs (4). Infection with this pathogen can lead to the disease ...
Chemotherapy can cause immunosuppression, which may trigger latent intestinal parasitic infections in stools to emerge. This study investigated whether intestinal parasites can emerge as opportunistic infections in breast and colorectal cancer patients (n=46 and n=15, respectively) undergoing chemotherapy treatment. Breast cancer patients were receiving a 5-fluorouracil/epirubicin/cyclophosphamide (FEC) regimen (6 chemotherapy cycles), and colorectal cancer patients were receiving either an oxaliplatin/5-fluorouracil/folinic acid (FOLFOX) regimen (12 cycles) or a 5-fluorouracil/folinic acid (Mayo) regimen (6 cycles). Patients had Blastocystis hominis and microsporidia infections that were only present during the intermediate chemotherapy cycles. Thus, cancer patients undergoing chemotherapy should be screened repeatedly for intestinal parasites, namely B. hominis and microsporidia, as they may reduce the efficacy of chemotherapy treatments.. ...
Blastocystis hominis is a common enteric parasite of worldwide distribution. Its pathogenetic potential has not yet been established, although numerous case reports suggest that B. hominis may cause the development of various gastrointestinal symptoms and disorders. The detection of the parasite in stool specimens is conventionally done by microscopy of direct smears, fecal concentrates, or permanently stained smears; however, morphology-based diagnosis is problematic. The aim of this study was to develop and evaluate a polymerase chain reaction (PCR) technique for the direct detection of B. hominis in human stool samples. Primers were based on small subunit ribosomal DNA and able to detect ≥32 parasites/200 mg stool artificially spiked with cultured B. hominis. In the evaluation of 43 clinical specimens, the PCR was tested against the formol ethyl acetate concentration technique (FECT) and a culture technique, proving 100% test specificity and a significantly higher sensitivity than the FECT. ...
Question - Does these stool test for Blastocystis hominis suggest anything about my chronic diarrhea?. Ask a Doctor about diagnosis, treatment and medication for Diarrhea, Ask a General & Family Physician
Blastocystis from humans and animals can be divided into at least 12 species, of which several are found in humans Recently, a 29-kDa parasite protein and a parasite-associated protease have received attention as potential markers of pathogenicity It has been suggested that finding |5 parasites per high-power field (40× objective) or, less commonly, by oil…
The impacts can not be the same for each individual and here and there the last outcomes dont need to be a risk to the life. In any case, you should realize that even the ones that dont speak to such a major issue for the wellbeing can have some not all that wonderful symptoms.. 8 Common Symptoms of Parasitic Infection. 1. Fatigue. Dr. Leo Galland,states that the inward parasites are the ones that are in charge of constant weakness that a hefty portion of his patients have.Mostly in this sort of cases, the no one but motivation can be the protozoa class of parasite, that can dwell just by drinking water.. 2. IBS. Almost half of all IBS patients have a parasite called blastocystis hominis. The oral-fecal contact makes this parasite spread.. For example, if a gourmet specialist does not wash his hands completely subsequent to utilizing the restroom, he will open the clients to conceivable blastocystis hominis disease.. 3. Skin Conditions. Mites are parasites that enter into your skin and lay ...
Hi Don.. Ill try to keep this brief, but I have noticed so many benefits from my Terminator Zapper in such a short period of time, my head is spinning! So you know a little bit about me - Im a 56 year old single male, retired at 53 and loving it!. About eight weeks ago, I was diagnosed with Blastocystis Hominis, a fairly common intestinal parasite. I probably had them for at least six weeks prior to diagnosis, based on how bad I was feeling. It took awhile to realize that something wasnt right. I was craving and eating mostly junk food because this is what the parasites craved. After eating, my stomach would be bloated and the contents would churn and churn as my millions of parasites were dining.. I currently do not have insurance, so $500 later in doctor bills and 7 days of antibiotics that did absolutely nothing to help the situation, except to make me feel worse, and I was done with traditional medicine. I hit my computer and I hit it hard - I had to find an alternate solution for my ...
This test gives an idea of how well foods are digested and absorbed, gives some products of gut fermentation, looks for blood in the stool, gives counts of bacteria and yeast, identifies those organisms which should not be there and lists antibiotic and/or antifungal preparations, both herbal and drug, to which that micro-organism is sensitive. It also looks for parasitic micro-organisms such as amoeba, blastocystis hominis, cryptosporydia and giardia lamblia and suggests possible treatment options. The stool analysis is carried out at Genova Diagnostics in the USA. ...
We have conducted follow-up analyses of the top 10% of SNPs from the ParAllele (Affymetrix) Immune and Inflammation panel in a new set of 584 cases and 768 controls, for a combined sample of 1,009 cases and 1,233 controls. We found that the common SNP rs241447 (MAF 0.26) in TAP2 from the 6p21.3 region showed a significant association with risk of NHL overall after correcting for multiple testing; the association was particularly strong for FL, but was also apparent for DLBCL. Higher TAP2 expression was associated with the risk allele in both FL and DLBCL tumors.. The 6p21.3 region is a large, complex, and immune gene-rich region that has been previously implicated as a susceptibility locus for overall NHL risk (5, 10-12, 15). Furthermore, this region has been flagged as a region of interest for not only for NHL, but also for the specific NHL subtypes of FL (10, 11, 13, 14), DLBCL (5, 10, 15), and familial CLL/SLL (16). In NHL subtype analyses, we found genome-wide significance for the TAP2 SNP ...
Subtype analysis and mutations to antiviral drugs in HIV-1-infected patients from Mozambique before initiation of antiretroviral therapy: Results from the DREAM programme ...
Protozoan parasites, including Blastocystis hominis, Dientamoeba fragilis and a few other amoebas such as Entamoeba Histolytica are single cell parasites which for years were thought to be harmless. During the last 10 to 12 years a number of medical reports have associated these parasites with various problems such as irritable bowel and chronic fatigue. It is interesting that Blastocystis and or Dientamoeba are found in 5% of the general population but up to 30% of people suffering with irritable bowel. Some of the clients contacting us have a history of an acute infection of the digestive track while travelling in the tropics or in countries with poor hygiene. These days many people contract these parasites in the UK ...
Get natural cures for Blastocystis Hominis Infection that can make a difference in your life or the life of someone you love with alternative treatments.
The stools and rectal biopsy specimens of 44 patients with AIDS and diarrhoea were examined by culture, light microscopy, and electron microscopy. In 13 patients examination of rectal biopsy material and faecal samples showed no pathogen, but in two of these, microsporidiosis was found by electron microscopical examination of jejunal biopsy specimens. This organism was also identified electron microscopically in one of the further five jejunal biopsy samples taken from patients with a known cause of diarrhoea. Blastocystis hominis infection was identified electron microscopically in six patients, all of whom had cryptosporidiosis additionally seen by light microscopy. Four of these six patients remained well for long periods, with only moderate diarrhoea, and follow up showed no evidence of blastocystis infection. In only four of 11 patients found to have cryptosporidium in their stools at light microscopy were organisms found at electron microscopy. Viral inclusions were only identified at ...
Intestinal parasites infections are a serious public health problem in the world and, in particular, in developing countries like Brazil. They are quite frequent in school-aged children influencing on growth and intellectual development. The data about the real situation of enteroparasitosis are scarce and epidemiological surveys are important not only for monitoring as in actions in preventive medicine. The present study evaluated the presence of intestinal parasites in a day care child center on the outskirts of the city of São Paulo. 21 stool samples were analyzed, being 16 children with an average age of 3 years, and 5 adults, daycare staff. The method used was the coproplus®. Of the total samples analyzed in 17 (81%) was established some kind of intestinal parasite Protozoan species only, and in 8 (47%) had more than one species of parasite. The frequency of the parasites found were: Blastocystis hominis (53%), Endolimax nana (35%), Giardia duodenalis (23%), Cryptosporidium parvum (12%), ...
This test is performed on stool samples collected over 3 days and looks for and identifies parasitic micro-organisms such as amoeba, blastocystis hominis, cryptosporydia and giardia lamblia etc. If a more detailed assessment of gut flora is needed, then Comprehensive parasitology might be useful. This test reports on bacterial and yeast flora as well as looking for parasites in the stool samples. It also provides an overview of available treatments for bacterial and yeast dysbiosis. The analysis is carried out at Genova Diagnostics in the USA. ...
1. Central Intelligence Agency. The 2008 coccidia, Cryptosporidium sp dengan https://www.cia.gov/library/publications/th 2. UNAIDS. AIDS Epidemic update: special 8. Duval X and Leport C. Toxoplasmosis in 9. Rao K, Sekar U, Iraivan KT, Abraham G, Soundararajan P. Blastocystis hominis - An 4. Saag MS, Graybill RJ, Larsen RA, Pappas Transplant Recipients. JAPI 2003, 51 :719- 10. Kurniawan A, Karyadi T, Dwintasari SW, Group Cryptococcal Subproject. Guidelines Sari IP, Yunihastuti E, Djauzi S, Smith HV. Intestinal parasitic infections in HIV/AIDS Management of Cryptococcal Disease. Clin Jakarta, Indonesia. Trans R Soc Trop Med and HIV Infection. http://hivinsite.ucsf.edu/ 11. Amadi B, Mwiya M, Musuku J, Watuka A, nitazoxanide on morbidity and mortality in Zambian children with cryptosporidiosis: a randomised controlled trial. Lancet.2002 Source: http://www.majalahfk.uki.ac.id/assets/majalahfile/artikel/2008-01-artikel-051.pdf ...
Blastocystis hominus is a parasite that is often found in the stools of healthy people and often recovered from persons having loose stools/GI complaints though it remains unclear whether the...
Blastocystosis is a medical condition caused by infection with Blastocystis, a single-celled parasite that infects the gastrointestinal tract of humans and animals.
CancerSubtypes is a package for cancer subtype analysis that includes various functions from dataset processing to result validation. In CancerSubtypes package, we provide a unified framework for analysing cancer subtpes from raw data to result visualisation. The main functions include genomic data pre-processing, cancer subtypes identification, results validation, visualization and comparison. CancerSubtypes provides the common data imputation and normalization methods for the genomic data pre-processing. Meanwhile, there are four feature selection methods to screen the key features in genomic dataset. The common cancer subtypes identification methods are integrated in this package such as Consensus clustering (CC) [From R package ConsensusClusterPlus], Consensus Nonnegative matrix factorization (CNMF) [From R package NMF], Integrative clustering (iCluster)[From R package iCluster], Similarity Network Fusion (SNF) [From R package SNFtool], Combined SNF and CC (SNF.CC) and Weighted Similarity ...
Entamoeba histolytica Entamoeba dispar Entamoeba moshkovskii Entamoeba bangladeshi Entamoeba hartmanni Entamoeba coli Entamoeba polecki Entamoeba gingivalis Endolimax nana Iodamoeba bütschlii Blastocystis spp.
Greetings, Eric Bakker, naturopath, author of Candida Crusher.. Today, Id like to do a video about parasites. Particularly about Blastocystis hominis and Dientamoeba fragilis. Now those names maybe sound a bit funny to you, and some people may even think that Im reading a few lines out of Harry Potter or a book like that. But I can tell you now; Im not really talking about witchcraft or giving you any false leads here.. Blastocystis hominis is a very common parasite found in third-world countries. But not just third world, we find it also in many western countries. In fact, its estimated that 25 to 30 percent of people with irritable bowel syndrome have a Blasto infection, so theyre relatively common. These are tiny little parasites and just like Candida albicans, Blasto lives in the digestive system of many, many different people, many healthy people, but it lives in balance and its kept in check by the beneficial bacteria. But when out of balance, Blasto can create a lot of problems for ...
The surfaces of massive corals of the genus Favia from Eilat, Red Sea, and from Heron Island, Great Barrier Reef, are covered by a layer of eukaryotic microorganisms. These microorganisms are embedded in the coral mucus and tissue. In the Gulf of Eilat, the prevalence of corals covered by patches of eukaryotic microorganisms was positively correlated with a decrease in water temperatures (from 25-28 degrees C in the summer to 20-23 degrees C in winter). Comparisons carried out using transmission and scanning electron microscopy showed morphological similarities between the microorganisms from the two geographically distant reefs. The microorganisms found on and in the tissues were approximately 5-15 microm in diameter, surrounded by scales in their cell wall, contained a nucleus, and included unique auto-florescent coccoid bodies of approximately 1 mum. Such morphological characters suggested that these microorganisms are stramenopile protists and in particular thraustochytrids. Molecular ...
Labile toxin producing enterotoxic E. coli (ETEC) were the commonest pathogen isolated from diarrheal stools of hospitalized children (21%) and adults (26%) in Singapore. Salmonellas ranked a close second in children (19%). Other bacterial pathogens were isolated from less than 5% of subjects. Blastocystis hominis was detected in 4.3% of diarrheal stools when a simple sedimentation technique was used. Cryptosporidium was not detected at all. An analysis of yeast counts in smears of diarrheal and non-diarrheal stools suggested they were etiologically associated with at least 6% of diarrhea in children and 19% in adults. Testing for rotaviruses by Latex agglutination and for adenovirus by electronmicroscopy showed an association with 6 per cent and 3 per cent diarrhea respectively. The study highlighted a need for: case control studies on ETEC and B. hominis; studies on the epidemiology of diarrhea by yeasts; establishing the true incidence of adenovirus diarrhea; studies on the prevalence and seasonality
Its well known among naturopaths and other alternative health practitioners that if one person in a household if infected by H pylori, other family or household members can also be infected.. In clinical practice I see this time and time again.. The trouble is that H pylori doesnt cause symptoms in everyone, so lets set the scene (this is a real life example from a family I worked with a few years ago).. Joyce has a whole bunch of digestive symptoms, but her husband, Colin, did not. Their daughter also had symptoms.. They all did a stool test like this one.. All three of them tested positive for H pylori, and both Joyce and her daughter tested positive for the Blastocystis hominis parasite.. This certainly isnt an isolated case - Ive seen H pylori in different household members on many occasions.. When more than one person in the household has H pylori, it can be passed from person-to-person.. Kissing, sexual contact and possibly even sharing eating and drinking utensils can lead to H ...
I have never tried a salt flush. I guess it gives you a good cleanse and perhaps kills or flushes out some parasites at the same time? The parasites that I know I have according to stool samples: Blastocystis hominis and Dientamoeba fragilis (many). Not sure if they could be related to the returning problem with hemorrhoids (external and internal) What i would like to know is if a salt flush is going to hurt? Salt in the wounds I guess will hurt (as the loose contents of salt comes out through the intestines? And can the parasites somehow be related to the recurring hemorrhoids?
Routine Clinical Chemistry Tests. Albumin, Alkaline Phosphatase, ALT/SGPT, Ammonia, Amylase, AST/SGOT, Bilirubin, Blood Gases, BUN (Blood Urea Nitrogen), Calcium, Cholesterol, Cholinesterase, Creatinine, CK-MB, Electrolytes (Carbon Dioxide/Bicarbonate, Chloride, Potassium, Sodium), Ferritin, Follate/Folic Acid, Fructosamine, GGT, Glucose, HDL, Iron, LDH, Magnesium, Phosphorus, Potassium, Protein, Sodium, Triglycerides, Troponin, Uric Acid, Urinalysis, Vitamin B12.. Infectious Disease Tests. AIDS/HIV, Adenovirus, Aeromonads, Bartonella, Blastocystis Hominis, Campylobacter, Candida, Chancroid, Chlamydia, Clostridium, Coronavirus, Coxsackievirus, Cryptosporidium, Cyclospora, CMV, E. Coli, Echovirus, Encephalitis, Enterovirus, EBV, Giardia, Gonorrhea, Granuloma Inguinale, Hantavirus, H. Pylori, Hepatitis (A, B, C, D, E), Herpes Simplex Virus, Human Herpes Virus-6 (HHV-6), Influenza, Legionella, Lyme disease, Pymphogranuloma, Malaria, Measles, Meningitis, Mononucleosis, Microsporidium, Mononucleosis, ...
Nitazoxanide is a broad-spectrum antiparasitic and broad-spectrum antiviral drug that is used in medicine for the treatment of various helminthic, protozoal, and viral infections. It is indicated for the treatment of infection by Cryptosporidium parvum and Giardia lamblia in immunocompetent individuals and has been repurposed for the treatment of influenza. Nitazoxanide has also been shown to have in vitro antiparasitic activity and clinical treatment efficacy for infections caused by other protozoa and helminths; emerging evidence suggests that it possesses efficacy in treating a number of viral infections as well. Chemically, nitazoxanide is the prototype member of the thiazolides, a class of drugs which are synthetic nitrothiazolyl-salicylamide derivatives with antiparasitic and antiviral activity. Tizoxanide, an active metabolite of nitazoxanide in humans, is also an antiparasitic drug of the thiazolide class. Nitazoxanide is an effective first-line treatment for infection by Blastocystis ...
Many of the parasites which have the greatest effect on our collective health and economy, and which have influenced the fates of nations, are protists. Most significantly, they include the agents of sleeping sickness and malaria. Sleeping sickness is caused by a kinetoplastid flagellate. The direct impact of sleeping sickness on humans is now much reduced, but in the 19th century, it wreaked destruction in Africa, facilitated by ill-informed moralistically motivated colonialists (Ford 1971). Malaria, caused by Plasmodium - an apicomplexan alveolate - remains the number one infectious disease affecting people.. Protists embrace many species which are found within invertebrate and vertebrate animals, within plants, or even within other protists. Some of these endobionts are clearly pathogenic, but the nature of the relationship between others seem to be more benign. At one end of the spectrum Phytophthora, a stramenopile, levels large tracts of native woodlands of Australia by invading the ...
Blastocystis and gut microbiota. *Back to Pegase biosciences. Category Archives: Non classé Post navigation. ← Older posts ...

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