Blastocystis: A genus of protozoa of the suborder BLASTOCYSTINA. It was first classified as a yeast but further studies have shown it to be a protozoan.SikkimIntestinal Diseases, Parasitic: Infections of the INTESTINES with PARASITES, commonly involving PARASITIC WORMS. Infections with roundworms (NEMATODE INFECTIONS) and tapeworms (CESTODE INFECTIONS) are also known as HELMINTHIASIS.Protozoan Infections: Infections with unicellular organisms formerly members of the subkingdom Protozoa.Feces: Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.Libya: A country in northern Africa, bordering the Mediterranean Sea, between Egypt, Tunisia, and Algeria, having southern border with Chad, Niger, and Sudan. Its capital is Tripoli.Parasitology: The study of parasites and PARASITIC DISEASES.DNA, Protozoan: Deoxyribonucleic acid that makes up the genetic material of protozoa.Succinate-CoA Ligases: Enzymes that catalyze the first step leading to the oxidation of succinic acid by the reversible formation of succinyl-CoA from succinate and CoA with the concomitant cleavage of ATP to ADP (EC 6.2.1.5) or GTP to GDP (EC 6.2.1.4) and orthophosphate. Itaconate can act instead of succinate and ITP instead of GTP.EC 6.2.1.-.Stramenopiles: A common name (but used formally) for a group of organisms that are mostly kinds of algae including BACILLARIOPHYTA; OOMYCETES; PHAEOPHYCEAE; and CHRYSOPHYCEAE. They all contain CHLOROPLASTS that are thought to have been derived from the endosymbiosis of ancient RED ALGAE.Metronidazole: A nitroimidazole used to treat AMEBIASIS; VAGINITIS; TRICHOMONAS INFECTIONS; GIARDIASIS; ANAEROBIC BACTERIA; and TREPONEMAL INFECTIONS. It has also been proposed as a radiation sensitizer for hypoxic cells. According to the Fourth Annual Report on Carcinogens (NTP 85-002, 1985, p133), this substance may reasonably be anticipated to be a carcinogen (Merck, 11th ed).Blastocystis hominis: A species of parasitic protozoa found in the intestines of humans and other primates. It was classified as a yeast in 1912. Over the years, questions arose about this designation. In 1967, many physiological and morphological B. hominis characteristics were reported that fit a protozoan classification. Since that time, other papers have corroborated this work and the organism is now recognized as a protozoan parasite of humans causing intestinal disease with potentially disabling symptoms.Digestive System Diseases: Diseases in any part of the GASTROINTESTINAL TRACT or the accessory organs (LIVER; BILIARY TRACT; PANCREAS).Blastocystis Infections: Infections with organisms of the genus BLASTOCYSTIS. The species B. hominis is responsible for most infections. Parasitologic surveys have generally found small numbers of this species in human stools, but higher positivity rates and organism numbers in AIDS patients and other immunosuppressed patients (IMMUNOCOMPROMISED HOST). Symptoms include ABDOMINAL PAIN; DIARRHEA; CONSTIPATION; VOMITING; and FATIGUE.Gastroenterology: A subspecialty of internal medicine concerned with the study of the physiology and diseases of the digestive system and related structures (esophagus, liver, gallbladder, and pancreas).Immunocompromised Host: A human or animal whose immunologic mechanism is deficient because of an immunodeficiency disorder or other disease or as the result of the administration of immunosuppressive drugs or radiation.Constipation: Infrequent or difficult evacuation of FECES. These symptoms are associated with a variety of causes, including low DIETARY FIBER intake, emotional or nervous disturbances, systemic and structural disorders, drug-induced aggravation, and infections.Diarrhea: An increased liquidity or decreased consistency of FECES, such as running stool. Fecal consistency is related to the ratio of water-holding capacity of insoluble solids to total water, rather than the amount of water present. Diarrhea is not hyperdefecation or increased fecal weight.Abdominal Pain: Sensation of discomfort, distress, or agony in the abdominal region.Crohn Disease: A chronic transmural inflammation that may involve any part of the DIGESTIVE TRACT from MOUTH to ANUS, mostly found in the ILEUM, the CECUM, and the COLON. In Crohn disease, the inflammation, extending through the intestinal wall from the MUCOSA to the serosa, is characteristically asymmetric and segmental. Epithelioid GRANULOMAS may be seen in some patients.Strongyloides ratti: A species of parasitic nematode found in rats.Giardia lamblia: A species of parasitic EUKARYOTES that attaches itself to the intestinal mucosa and feeds on mucous secretions. The organism is roughly pear-shaped and motility is somewhat erratic, with a slow oscillation about the long axis.Fluorescence: The property of emitting radiation while being irradiated. The radiation emitted is usually of longer wavelength than that incident or absorbed, e.g., a substance can be irradiated with invisible radiation and emit visible light. X-ray fluorescence is used in diagnosis.Giardia: A genus of flagellate intestinal EUKARYOTES parasitic in various vertebrates, including humans. Characteristics include the presence of four pairs of flagella arising from a complicated system of axonemes and cysts that are ellipsoidal to ovoidal in shape.Cryptosporidium parvum: A species of parasitic protozoa that infects humans and most domestic mammals. Its oocysts measure five microns in diameter. These organisms exhibit alternating cycles of sexual and asexual reproduction.GlycogenOregonVeterinary Medicine: The medical science concerned with the prevention, diagnosis, and treatment of diseases in animals.Eukaryota: One of the three domains of life (the others being BACTERIA and ARCHAEA), also called Eukarya. These are organisms whose cells are enclosed in membranes and possess a nucleus. They comprise almost all multicellular and many unicellular organisms, and are traditionally divided into groups (sometimes called kingdoms) including ANIMALS; PLANTS; FUNGI; and various algae and other taxa that were previously part of the old kingdom Protista.Dientamoeba: A genus of minute EUKARYOTES that are characterized by the preponderance of binucleate over uninucleate forms, the presence of several distinct granules in the karyosome, and the lack of a cystic stage. It is parasitic in the large intestine of humans and certain monkeys.Dientamoebiasis: Gastrointestinal infection with organisms of the genus DIENTAMOEBA.Entamoeba histolytica: A species of parasitic protozoa causing ENTAMOEBIASIS and amebic dysentery (DYSENTERY, AMEBIC). Characteristics include a single nucleus containing a small central karyosome and peripheral chromatin that is finely and regularly beaded.Irritable Bowel Syndrome: A disorder with chronic or recurrent colonic symptoms without a clearcut etiology. This condition is characterized by chronic or recurrent ABDOMINAL PAIN, bloating, MUCUS in FECES, and an erratic disturbance of DEFECATION.Cytokines: Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.Colonic Diseases, Functional: Chronic or recurrent colonic disorders without an identifiable structural or biochemical explanation. The widely recognized IRRITABLE BOWEL SYNDROME falls into this category.Enteritis: Inflammation of any segment of the SMALL INTESTINE.Research Support as Topic: Financial support of research activities.Toilet Facilities: Facilities provided for human excretion, often with accompanying handwashing facilities.Swine Diseases: Diseases of domestic swine and of the wild boar of the genus Sus.Dysentery: Acute inflammation of the intestine associated with infectious DIARRHEA of various etiologies, generally acquired by eating contaminated food containing TOXINS, BIOLOGICAL derived from BACTERIA or other microorganisms. Dysentery is characterized initially by watery FECES then by bloody mucoid stools. It is often associated with ABDOMINAL PAIN; FEVER; and DEHYDRATION.Intestinal Diseases: Pathological processes in any segment of the INTESTINE from DUODENUM to RECTUM.Software: Sequential operating programs and data which instruct the functioning of a digital computer.Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.Parasites: Invertebrate organisms that live on or in another organism (the host), and benefit at the expense of the other. Traditionally excluded from definition of parasites are pathogenic BACTERIA; FUNGI; VIRUSES; and PLANTS; though they may live parasitically.Algorithms: A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.User-Computer Interface: The portion of an interactive computer program that issues messages to and receives commands from a user.Pseudohypoparathyroidism: A hereditary syndrome clinically similar to HYPOPARATHYROIDISM. It is characterized by HYPOCALCEMIA; HYPERPHOSPHATEMIA; and associated skeletal development impairment and caused by failure of response to PARATHYROID HORMONE rather than deficiencies. A severe form with resistance to multiple hormones is referred to as Type 1a and is associated with maternal mutant allele of the ALPHA CHAIN OF STIMULATORY G PROTEIN.Computer Graphics: The process of pictorial communication, between human and computers, in which the computer input and output have the form of charts, drawings, or other appropriate pictorial representation.Paromomycin: An oligosaccharide antibiotic produced by various STREPTOMYCES.Oocyte Donation: Transfer of preovulatory oocytes from donor to a suitable host. Oocytes are collected, fertilized in vitro, and transferred to a host that can be human or animal.Appointments and Schedules: The different methods of scheduling patient visits, appointment systems, individual or group appointments, waiting times, waiting lists for hospitals, walk-in clinics, etc.Water: A clear, odorless, tasteless liquid that is essential for most animal and plant life and is an excellent solvent for many substances. The chemical formula is hydrogen oxide (H2O). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Faith Healing: The use of faith and spirit to cure disease.Tissue and Organ Procurement: The administrative procedures involved with acquiring TISSUES or organs for TRANSPLANTATION through various programs, systems, or organizations. These procedures include obtaining consent from TISSUE DONORS and arranging for transportation of donated tissues and organs, after TISSUE HARVESTING, to HOSPITALS for processing and transplantation.Directed Tissue Donation: Tissue, organ, or gamete donation intended for a designated recipient.

Blastocystis isolates from a pig and a horse are closely related to Blastocystis hominis. (1/51)

Blastocystis has a widespread distribution in a variety of animals, which is a potential source of infection for humans. However, the contribution of zoonotic transmission remains unclear due to the absence of molecular proof of these organisms being identical to those found in humans. We report herein the similar subgroup of Blastocystis isolates from humans, pigs, and a horse using a restriction fragment length polymorphism (RFLP) analysis of partial small-subunit ribosomal DNA (ssu rDNA). Additionally, sequence and phylogenic analysis of partial ssu rDNA of Blastocystis from a human, a pig, and a horse sharing a common subgroup shows that Blastocystis isolates from a pig and a horse were monophyletic and closely related to B. hominis, with 92 to 94% identity. These results suggest the possibility of zoonotic potential of Blastocystis.  (+info)

PCR-based identification of zoonotic isolates of Blastocystis from mammals and birds. (2/51)

The genotype of Blastocystis isolated from humans and animals is highly polymorphic. Therefore, it is important to compare the genotypes of Blastocystis isolates from humans and animals to determine the zoonotic potential of animal isolates. PCR-based genotype classification using known sequence-tagged site (STS) primers allows identification of zoonotic isolates of animal origin. To this end, 51 isolates from monkeys, cattle, pigs, chickens, quails and pheasants were subjected to genotype analysis using seven kinds of STS primers. Out of the 51 isolates, 39 were identified as one of the known genotypes, four showed mixed genotypes, and eight were unknown genotypes as these were negative for all STS primers. When these results were combined with previous studies on 41 isolates from animals and compared with the diversity of genotypes of 102 human Blastocystis hominis isolates, 67.4 % (62/92) of isolates from mammals and birds were identical to human B. hominis genotypes. Since the unknown genotype of human origin had been placed into an additional clade in the small-subunit rRNA gene phylogeny, further molecular study on the eight isolates of unknown genotype from the present study will facilitate our understanding of their zoonotic potential.  (+info)

Molecular phylogenies of Blastocystis isolates from different hosts: implications for genetic diversity, identification of species, and zoonosis. (3/51)

Small-subunit (SSU) rRNA gene sequences were obtained by PCR from 12 Blastocystis isolates from humans, rats, and reptiles for which elongation factor 1alpha (EF-1alpha) gene sequences are already available. These new sequences were analyzed by the Bayesian method in a broad phylogeny including, for the first time, all Blastocystis sequences available in the databases. Phylogenetic trees identified seven well-resolved groups plus several discrete lineages that could represent newly defined clades. Comparative analysis of SSU rRNA- and EF-1alpha-based trees obtained by maximum-likelihood methods from a restricted sampling (13 isolates) revealed overall agreement between the two phylogenies. In spite of their morphological similarity, sequence divergence among Blastocystis isolates reflected considerable genetic diversity that could be correlated with the existence of potentially >/=12 different species within the genus. Based on this analysis and previous PCR-based genotype classification data, six of these major groups might consist of Blastocystis isolates from both humans and other animal hosts, confirming the low host specificity of Blastocystis. Our results also strongly suggest the existence of numerous zoonotic isolates with frequent animal-to-human and human-to-animal transmissions and of a large potential reservoir in animals for infections in humans.  (+info)

Viable blastocystis cysts in Scottish and Malaysian sewage samples. (4/51)

Blastocystis cysts were detected in 38% (47/123) (37 Scottish, 17 Malaysian) of sewage treatment works. Fifty percent of influents (29% Scottish, 76% Malaysian) and 28% of effluents (9% Scottish, 60% Malaysian) contained viable cysts. Viable cysts, discharged in effluent, provide further evidence for the potential for waterborne transmission of Blastocystis.  (+info)

Blastocystis ratti induces contact-independent apoptosis, F-actin rearrangement, and barrier function disruption in IEC-6 cells. (5/51)

Blastocystis is an enteric protozoan purportedly associated with numerous clinical cases of diarrhea, flatulence, vomiting, and other gastrointestinal symptoms. Despite new knowledge of Blastocystis cell biology, genetic diversity, and epidemiology, its pathogenic potential remains controversial. Numerous clinical and epidemiological studies either implicate or exonerate the parasite as a cause of intestinal disease. Therefore, the aim of this study was to investigate the pathogenic potential of Blastocystis by studying the interactions of Blastocystis ratti WR1, an isolate of zoonotic potential, with a nontransformed rat intestinal epithelial cell line, IEC-6. Here, we report that B. ratti WR1 induces apoptosis in IEC-6 cells in a contact-independent manner. Furthermore, we found that B. ratti WR1 rearranges F-actin distribution, decreases transepithelial resistance, and increases epithelial permeability in IEC-6 cell monolayers. In addition, we found that the effects of B. ratti on transepithelial electrical resistance and epithelial permeability were significantly abrogated by treatment with metronidazole, an antiprotozoal drug. Our results suggest for the first time that Blastocystis-induced apoptosis in host cells and altered epithelial barrier function might play an important role in the pathogenesis of Blastocystis infections and that metronidazole has therapeutic potential in alleviating symptoms associated with Blastocystis.  (+info)

Cellular identification of a novel uncultured marine stramenopile (MAST-12 Clade) small-subunit rRNA gene sequence from a norwegian estuary by use of fluorescence in situ hybridization-scanning electron microscopy. (6/51)

Revealing the cellular identity of organisms behind environmental eukaryote rRNA gene sequences is a major objective in microbial diversity research. We sampled an estuarine oxygen-depleted microbial mat in southwestern Norway and retrieved an 18S rRNA gene signature that branches in the MAST-12 clade, an environmental marine stramenopile clade. Detailed phylogenetic analyses revealed that MAST-12 branches among the heterotrophic stramenopiles as a sister of the free-living Bicosoecida and the parasitic genus Blastocystis. Specific sequence signatures confirmed a relationship to these two groups while excluding direct assignment. We designed a specific oligonucleotide probe for the target sequence and detected the corresponding organism in incubation samples using fluorescence in situ hybridization (FISH). Using the combined FISH-scanning electron microscopy approach (T. Stoeck, W. H. Fowle, and S. S. Epstein, Appl. Environ. Microbiol. 69:6856-6863, 2003), we determined the morphotype of the target organism among the very diverse possible morphologies of the heterotrophic stramenopiles. The unpigmented cell is spherical and about 5 mum in diameter and possesses a short flagellum and a long flagellum, both emanating anteriorly. The long flagellum bears mastigonemes in a characteristic arrangement, and its length (30 mum) distinguishes the target organism from other recognized heterotrophic stramenopiles. The short flagellum is naked and often directed posteriorly. The organism possesses neither a lorica nor a stalk. The morphological characteristics that we discovered should help isolate a representative of a novel stramenopile group, possibly at a high taxonomic level, in order to study its ultrastructure, physiological capabilities, and ecological role in the environment.  (+info)

Blastocystis ratti contains cysteine proteases that mediate interleukin-8 response from human intestinal epithelial cells in an NF-kappaB-dependent manner. (7/51)

Blastocystis is a ubiquitous enteric protozoan found in the intestinal tracts of humans and a wide range of animals. Evidence accumulated over the last decade suggests association of Blastocystis with gastrointestinal disorders involving diarrhea, abdominal pain, constipation, nausea, and fatigue. Clinical and experimental studies have associated Blastocystis with intestinal inflammation, and it has been shown that Blastocystis has potential to modulate the host immune response. Blastocystis is also reported to be an opportunistic pathogen in immunosuppressed patients, especially those suffering from AIDS. However, nothing is known about the parasitic virulence factors and early events following host-parasite interactions. In the present study, we investigated the molecular mechanism by which Blastocystis activates interleukin-8 (IL-8) gene expression in human colonic epithelial T84 cells. We demonstrate for the first time that cysteine proteases of Blastocystis ratti WR1, a zoonotic isolate, can activate IL-8 gene expression in human colonic epithelial cells. Furthermore, we show that NF-kappaB activation is involved in the production of IL-8. In addition, our findings show that treatment with the antiprotozoal drug metronidazole can avert IL-8 production induced by B. ratti WR1. We also show for the first time that the central vacuole of Blastocystis may function as a reservoir for cysteine proteases. Our findings will contribute to an understanding of the pathobiology of a poorly studied parasite whose public health importance is increasingly recognized.  (+info)

Organelles in Blastocystis that blur the distinction between mitochondria and hydrogenosomes. (8/51)

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To determine the distribution of Blastocystis sp. subtypes from Blastocystis cyst excreters, 1,000 fecal samples from patients suspected of enteroparasitic disease were scored for stool consistency, submitted to xenic in vitro culture (XIVC), formol ethyl acetate concentration (FECT) with subsequent isopycnic centrifugation, and polymerase chain reaction (PCR) with subtype (ST) analysis. Blastocystis was significantly more prevalent in specimens from patients with travel-associated diarrhea (15.6%) than those with persistent diarrhea (8.3%) (P = 0.005). Overall, 115 (11.5%) and 35 (3.5%) specimens were positive by XIVC and FECT, respectively. Blastocystis cysts were detected in 33 (28.7%) of the XIVC-positive specimens. A positive FECT result was associated with ST3 (P = 0.05). The presence of Blastocystis in general or Blastocystis cysts was independent of stool consistency, and no particular ST was significantly associated with cyst identification. In view of these data, the present study indicates
We determined cytokines (e.g. interleukin-8, 10, 12 and TNF-α) expression by peripheral blood mononuclear cells (PBMCs) and in rectal mucosa in diarrhoea-predominant irritable bowel syndrome (D-IBS) with Blastocystis spp. Eighty patients with D-IBS and Blastocystis spp. infection were classified as cases and 80 with D-IBS without Blastocystis spp. infection were classified as control. Cases were subdivided into D-IBS and Blastocystis sp. defined type 1 (subtype-specific primer SB83) and type 3 (SB227). Stool microscopy and culture were performed. Rectal biopsies were obtained for histology and cytokines by real-time PCR for mRNA expression of cytokines. PBMCs IL-8 was similar in different groups but in type 1, IL-8mRNA was increased compared with type 3 (P = 0·001) and control (P = 0·001). In type 1, IL-10 by PBMCs had a low mean value (14·5±1·6) compared with (16·7±1·5) type 3 and (16±2·3) in controls (P
Abstract This study aimed to elucidate aspects of the epidemiology of Blastocystis in Nigerian school children, including the distribution of subtypes (STs) and ST alleles. A total of 199 genomic DNAs extracted from fecal samples from 199 Nigerian children aged 2-14 years were tested by real-time polymerase chain reaction for Blastocystis. Positive DNAs were submitted to barcoding by PCR and sequencing to obtain information on STs and ST alleles. A total of 167 (84%) samples were positive for Blastocystis, with prevalence increasing by age. No association between Blastocystis colonization and gender (P = 0.51) or type/presence of toilet facilities (P = 0.21) was observed. Blastocystis carriers were more prone to using water collected from wells than from sachets (P = 0.0044). Moreover, Blastocystis positivity was associated with positivity for fecal-orally transmitted protozoa (P = 0.018) and helminths (P < 0.0001). A clear inverse association of Blastocystis colonization and malaria infection was
Wawrzyniak, Ivan, Courtine, Damien, Osman, Marwan S., Hubans-Pierlot, Christine, Cian, Amandine, Nourrisson, Celine, Chabe, Magali, Poirier, Philippe, Bart, Aldert, Polonais, Valerie, Delgado-Viscogliosi, Pilar, El Alaoui, Hicham, Belkorchia, Abdel, van Gool, Tom, Tan, Shyong Wei Kevin, Ferreira, Stephanie, Viscoglios, Eric, Delbac, Frederic. (2015). Draft genome sequence of the intestinal parasite Blastocystis subtype 4-isolate WR1. Genomics Data 4 : 22-23. [email protected] Repository. https://doi.org/10.1016/j.gdata.2015.01. ...
Although Blastocystis is one of the most common enteric parasites, there is still much controversy surrounding the pathogenicity and potential treatment options for this parasite. In this review we look at the evidence supporting Blastocystis as an intestinal pathogen as shown by numerous case studies and several in vivo studies and the evidence against. We describe the chronic nature of some infections and show the role of Blastocystis in immunocompromised patients and the relationship between irritable bowel syndrome and Blastocystis infection. There have been several studies that have suggested that pathogenicity may be subtype related. Metronidazole is the most widely accepted treatment for Blastocystis but several cases of treatment failure and resistance have been described. Other treatment options which have been suggested include paromomycin and trimethroprim- sulfamethoxazole.
Blastocystis hominis is the most common intestinal protozoan infecting humans in the United States, but it is probably the least understood. The parasite is also the most frequently diagnosed intestinal protozoan in Oregon. Records from the Good Samaritan Regional Medical Center, Corvallis, diagnostic laboratory show 40 cases of Blastocystis in 2006 vs. 10 for Giardia lamblia, a usual cause of gastrointestinal symptoms. Indeed, the prevalence of the infection might be much higher as routine methods used to detect other intestinal protozoans often miss Blastocystis.1 2 Blastocystis has been a medical mystery for years. Many people who carry the organism show no symptoms, while others inexplicably become very ill. Recent research has identified multiple genetically diverse sub-types of Blastocystis which can infect humans,3 and suggested only a specific sub-group is responsible for the disease.4 5 This model would be consistent with research suggesting a phylogenetic link between Blastocystis and
Blastocystosis refers to a medical condition caused by infection with Blastocystis. Blastocystis is a protozoal, single-celled parasite that inhabits the gastrointestinal tracts of humans and other animals. Many different types of Blastocystis exist, and they can infect humans, farm animals, birds, rodents, amphibians, reptiles, fish, and even cockroaches. Blastocystosis has been found to be a possible risk factor for development of IBS (Irritable Bowel Syndrome). There are conflicting reports regarding whether Blastocystis causes disease in humans. These reports resulted in a brief debate in medical journals in the early 1990s between some physicians in the United States who believed that Blastocystis was harmless, and physicians in the United States and overseas who believed it could cause disease. At the time, it was common practice to identify all Blastocystis from humans as Blastocystis hominis, while Blastocystis from animals was identified differently (e.g. Blastocystis ratti from rats). ...
A recently described cytotoxic monoclonal antibody (mAb 1D5) raised against Blastocystis hominis isolate B, was tested for reactivity with 13 different isolates of Blastocystis. The isolates used were previously isolated from humans, rats and reptiles and were maintained as axenised cultures throughout the course of this stady. Five B. hominis isolates (B, C, E, G and H) were found to react with mAb 1D5 in immunoblotting studies and the indirect fluorescence antibody test. The pattern of fluorescence observed for all five isolates was diffuse and patchy. Immunoblotting studies revealed that mAb 1D5 reacted with a 29-30-kDa protein found in all five isolates. Results of a cytotoxic assay showed that the mAb exhibited a complement-independent cytotoxic effect on all the exposed isolates. Microscopic observations showed differences in morphology between the Blastocystis cells exposed and unexposed to mAb. Acridine orange staining performed on both exposed and unexposed cells showed similar internal ...
Infections with organisms of the genus BLASTOCYSTIS. The species B. hominis is responsible for most infections. Parasitologic surveys have generally found small numbers of this species in human stools, but higher positivity rates and organism numbers in AIDS patients and other immunosuppressed patients (IMMUNOCOMPROMISED HOST). Symptoms include ABDOMINAL PAIN; DIARRHEA; CONSTIPATION; VOMITING; and FATIGUE ...
Hulda Clark suggested that all autoimmune diseases were actually caused by fluke parasites and pollutants. yes she has some good ideas, but I think way to simplified...she blames way to much on flukes, very few people in west have over flukes I think...one can test for several of them, and many people tried fluke medicines without getting better I think she has overlooked things like blastocystis hominis,and also her knowledge about diets seems limited... also her idea that basically all infections can be cured by zappers seems to be just an idea with little evidence...yes I wou ...
Hello, to anyone who is looking for a parasite treatment for Blastocystis Hominis, try looking up the website badbugs org, or the website for the Centre for Digestive Diseases in Sydney Australia. A ne...
Blastocystis Is Associated with Decrease of Fecal Microbiota Protective Bacteria: Comparative Analysis between Patients with Irritable Bowel Syndrome and Control Subjects. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
mark rosal ,rosal at mail.netshop.net, writes: ,Hello to everyone, I have rather peristent case of blasocystis hominis, ,and the regular medicines- such a flagy dont seem to be able to get rid ,of it. Can any body help me on this one. I am willing to look at any ,thing if it can specifically knock this bug out. Any and all ,information, suggestions, contacts would be greatly appreciated. Thanks ,--- mark rosal Blastocystis infection is probably the most common parasitic = infection in the world. Some surveys indicate infection rates of 10% in Europe and North America and up to 50% in developing countries. Relatively little is known about Blastocystis hominis compared to = most parasites and not everyone agrees on all points. Approximately = equal numbers of doctors think that it causes disease as those who = do not believe it does. The reason for this is quite simply that = most people infected show no symptoms at all that can be linked to = the presence of the parasite. In any event, the most ...
Jundishapur Journal of Microbiology Official Publication of School of Medicine, Ahvaz Jundishapur University of Medical Sciences ...
Omar O. Barriga (oobarrig at pop.service.ohio-state.edu) wrote: ,Blastocystis is not a pathogenic organism. This is far from being a settled issue. I would estimate that there are about an equal numbers of paper in the literature that say it is a pathogen as papers that say it is not. It may not cause invasive or fatal disease but that does not mean it is a commensal. It is also clear from the recent literature that human Blastocystis is actually two or more distinct organisms - this may in part explain the variable results being reported with respect to its role as a pathogen. Graham ____________________ C. Graham Clark, Ph.D. Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, England, G.B. Tel: ++44-171-927-2351 FAX: ++44-171-636-8739 e-mail: g.clark at lshtm.ac.uk ...
By Michael Biamonte, C.C.N. Several years ago, the Centers for Disease Control in Atlanta, Georgia along with some other experts and authorities, re-classified an organism known as Blastocystis Hominis-a common organism found in the intestinal tract of most people-as a dangerous disease-producing organism, as opposed to just a harmless organism. … ...
Tan, T.C.; Ong, S.C.; Suresh, K.G. (2009) Genetic variability of Blastocystis sp isolates obtained from cancer and HIV/AIDS patients. Parasitology Research, 105 (5). pp. 1283-1286. ISSN 0932-0113. Tan, T.C.; Suresh, K.G. (2006) Amoeboid form of Blastocystis hominis - a detailed ultrastructural insight. Parasitology Research, 99 (6). pp. 737-742. ISSN 0932-0113. Tan, T.C.; Suresh, K.G. (2007) Evidence of plasmotomy in Blastocystis hominis. Parasitology Research, 101 (6). pp. 1521-1525. ISSN 0932-0113. Tan, T.C.; Suresh, K.G. (2006) Predominance of amoeboid forms of Blastocystis hominis in isolates from symptomatic patients. Parasitology Research, 98 (3). pp. 189-193. ISSN 0932-0113. Tan, T.C.; Suresh, K.G.; Smith, H.V. (2008) Phenotypic and genotypic characterisation of Blastocystis hominis isolates implicates subtype 3 as a subtype with pathogenic potential. Parasitology Research, 104 (1). pp. 85-93. ISSN 0932-0113. Tan, T.C.; Suresh, K.G.; Thong, K.L.; Smith, H.V. (2006) PCR fingerprinting of ...
Below are some of the more common parasitic infections:. Blastocystis hominis. Although some consider Blastocystis hominis to be a commensal parasite, the more recent research shows that this is a pathogenic parasite. In addition, the research shows that Blastocystis hominis can cause an increase in inflammation (1), and even increase the permeability of the small intestines (2). A case report involving a 49-year old man with Hashimotos Thyroiditis shows that eradicating Blastocystis hominis resulted in a decrease of his thyroid antibodies (3). I personally have had a few patients with Hashimotos test positive for Blastocystis hominis and go into remission upon eradicating it.. Entamoeba histolytica. This is a pathogenic parasite that is associated with intestinal and extraintestinal infections (4). In other words, it not only can affect the intestines, but it can also affect extraintestinal sites such as the liver, brain, and lungs (4). Infection with this pathogen can lead to the disease ...
Question - Does these stool test for Blastocystis hominis suggest anything about my chronic diarrhea?. Ask a Doctor about diagnosis, treatment and medication for Diarrhea, Ask a General & Family Physician
Blastocystis from humans and animals can be divided into at least 12 species, of which several are found in humans Recently, a 29-kDa parasite protein and a parasite-associated protease have received attention as potential markers of pathogenicity It has been suggested that finding |5 parasites per high-power field (40× objective) or, less commonly, by oil…
The impacts can not be the same for each individual and here and there the last outcomes dont need to be a risk to the life. In any case, you should realize that even the ones that dont speak to such a major issue for the wellbeing can have some not all that wonderful symptoms.. 8 Common Symptoms of Parasitic Infection. 1. Fatigue. Dr. Leo Galland,states that the inward parasites are the ones that are in charge of constant weakness that a hefty portion of his patients have.Mostly in this sort of cases, the no one but motivation can be the protozoa class of parasite, that can dwell just by drinking water.. 2. IBS. Almost half of all IBS patients have a parasite called blastocystis hominis. The oral-fecal contact makes this parasite spread.. For example, if a gourmet specialist does not wash his hands completely subsequent to utilizing the restroom, he will open the clients to conceivable blastocystis hominis disease.. 3. Skin Conditions. Mites are parasites that enter into your skin and lay ...
Hi Don.. Ill try to keep this brief, but I have noticed so many benefits from my Terminator Zapper in such a short period of time, my head is spinning! So you know a little bit about me - Im a 56 year old single male, retired at 53 and loving it!. About eight weeks ago, I was diagnosed with Blastocystis Hominis, a fairly common intestinal parasite. I probably had them for at least six weeks prior to diagnosis, based on how bad I was feeling. It took awhile to realize that something wasnt right. I was craving and eating mostly junk food because this is what the parasites craved. After eating, my stomach would be bloated and the contents would churn and churn as my millions of parasites were dining.. I currently do not have insurance, so $500 later in doctor bills and 7 days of antibiotics that did absolutely nothing to help the situation, except to make me feel worse, and I was done with traditional medicine. I hit my computer and I hit it hard - I had to find an alternate solution for my ...
This test gives an idea of how well foods are digested and absorbed, gives some products of gut fermentation, looks for blood in the stool, gives counts of bacteria and yeast, identifies those organisms which should not be there and lists antibiotic and/or antifungal preparations, both herbal and drug, to which that micro-organism is sensitive. It also looks for parasitic micro-organisms such as amoeba, blastocystis hominis, cryptosporydia and giardia lamblia and suggests possible treatment options. The stool analysis is carried out at Genova Diagnostics in the USA. ...
We have conducted follow-up analyses of the top 10% of SNPs from the ParAllele (Affymetrix) Immune and Inflammation panel in a new set of 584 cases and 768 controls, for a combined sample of 1,009 cases and 1,233 controls. We found that the common SNP rs241447 (MAF 0.26) in TAP2 from the 6p21.3 region showed a significant association with risk of NHL overall after correcting for multiple testing; the association was particularly strong for FL, but was also apparent for DLBCL. Higher TAP2 expression was associated with the risk allele in both FL and DLBCL tumors.. The 6p21.3 region is a large, complex, and immune gene-rich region that has been previously implicated as a susceptibility locus for overall NHL risk (5, 10-12, 15). Furthermore, this region has been flagged as a region of interest for not only for NHL, but also for the specific NHL subtypes of FL (10, 11, 13, 14), DLBCL (5, 10, 15), and familial CLL/SLL (16). In NHL subtype analyses, we found genome-wide significance for the TAP2 SNP ...
Subtype analysis and mutations to antiviral drugs in HIV-1-infected patients from Mozambique before initiation of antiretroviral therapy: Results from the DREAM programme ...
Protozoan parasites, including Blastocystis hominis, Dientamoeba fragilis and a few other amoebas such as Entamoeba Histolytica are single cell parasites which for years were thought to be harmless. During the last 10 to 12 years a number of medical reports have associated these parasites with various problems such as irritable bowel and chronic fatigue. It is interesting that Blastocystis and or Dientamoeba are found in 5% of the general population but up to 30% of people suffering with irritable bowel. Some of the clients contacting us have a history of an acute infection of the digestive track while travelling in the tropics or in countries with poor hygiene. These days many people contract these parasites in the UK ...
The stools and rectal biopsy specimens of 44 patients with AIDS and diarrhoea were examined by culture, light microscopy, and electron microscopy. In 13 patients examination of rectal biopsy material and faecal samples showed no pathogen, but in two of these, microsporidiosis was found by electron microscopical examination of jejunal biopsy specimens. This organism was also identified electron microscopically in one of the further five jejunal biopsy samples taken from patients with a known cause of diarrhoea. Blastocystis hominis infection was identified electron microscopically in six patients, all of whom had cryptosporidiosis additionally seen by light microscopy. Four of these six patients remained well for long periods, with only moderate diarrhoea, and follow up showed no evidence of blastocystis infection. In only four of 11 patients found to have cryptosporidium in their stools at light microscopy were organisms found at electron microscopy. Viral inclusions were only identified at ...
Intestinal parasites infections are a serious public health problem in the world and, in particular, in developing countries like Brazil. They are quite frequent in school-aged children influencing on growth and intellectual development. The data about the real situation of enteroparasitosis are scarce and epidemiological surveys are important not only for monitoring as in actions in preventive medicine. The present study evaluated the presence of intestinal parasites in a day care child center on the outskirts of the city of São Paulo. 21 stool samples were analyzed, being 16 children with an average age of 3 years, and 5 adults, daycare staff. The method used was the coproplus®. Of the total samples analyzed in 17 (81%) was established some kind of intestinal parasite Protozoan species only, and in 8 (47%) had more than one species of parasite. The frequency of the parasites found were: Blastocystis hominis (53%), Endolimax nana (35%), Giardia duodenalis (23%), Cryptosporidium parvum (12%), ...
This test is performed on stool samples collected over 3 days and looks for and identifies parasitic micro-organisms such as amoeba, blastocystis hominis, cryptosporydia and giardia lamblia etc. If a more detailed assessment of gut flora is needed, then Comprehensive parasitology might be useful. This test reports on bacterial and yeast flora as well as looking for parasites in the stool samples. It also provides an overview of available treatments for bacterial and yeast dysbiosis. The analysis is carried out at Genova Diagnostics in the USA. ...
1. Central Intelligence Agency. The 2008 coccidia, Cryptosporidium sp dengan https://www.cia.gov/library/publications/th 2. UNAIDS. AIDS Epidemic update: special 8. Duval X and Leport C. Toxoplasmosis in 9. Rao K, Sekar U, Iraivan KT, Abraham G, Soundararajan P. Blastocystis hominis - An 4. Saag MS, Graybill RJ, Larsen RA, Pappas Transplant Recipients. JAPI 2003, 51 :719- 10. Kurniawan A, Karyadi T, Dwintasari SW, Group Cryptococcal Subproject. Guidelines Sari IP, Yunihastuti E, Djauzi S, Smith HV. Intestinal parasitic infections in HIV/AIDS Management of Cryptococcal Disease. Clin Jakarta, Indonesia. Trans R Soc Trop Med and HIV Infection. http://hivinsite.ucsf.edu/ 11. Amadi B, Mwiya M, Musuku J, Watuka A, nitazoxanide on morbidity and mortality in Zambian children with cryptosporidiosis: a randomised controlled trial. Lancet.2002 Source: http://www.majalahfk.uki.ac.id/assets/majalahfile/artikel/2008-01-artikel-051.pdf ...
Blastocystis hominus is a parasite that is often found in the stools of healthy people and often recovered from persons having loose stools/GI complaints though it remains unclear whether the...
Blastocystosis is a medical condition caused by infection with Blastocystis, a single-celled parasite that infects the gastrointestinal tract of humans and animals.
CancerSubtypes is a package for cancer subtype analysis that includes various functions from dataset processing to result validation. In CancerSubtypes package, we provide a unified framework for analysing cancer subtpes from raw data to result visualisation. The main functions include genomic data pre-processing, cancer subtypes identification, results validation, visualization and comparison. CancerSubtypes provides the common data imputation and normalization methods for the genomic data pre-processing. Meanwhile, there are four feature selection methods to screen the key features in genomic dataset. The common cancer subtypes identification methods are integrated in this package such as Consensus clustering (CC) [From R package ConsensusClusterPlus], Consensus Nonnegative matrix factorization (CNMF) [From R package NMF], Integrative clustering (iCluster)[From R package iCluster], Similarity Network Fusion (SNF) [From R package SNFtool], Combined SNF and CC (SNF.CC) and Weighted Similarity ...
Greetings, Eric Bakker, naturopath, author of Candida Crusher.. Today, Id like to do a video about parasites. Particularly about Blastocystis hominis and Dientamoeba fragilis. Now those names maybe sound a bit funny to you, and some people may even think that Im reading a few lines out of Harry Potter or a book like that. But I can tell you now; Im not really talking about witchcraft or giving you any false leads here.. Blastocystis hominis is a very common parasite found in third-world countries. But not just third world, we find it also in many western countries. In fact, its estimated that 25 to 30 percent of people with irritable bowel syndrome have a Blasto infection, so theyre relatively common. These are tiny little parasites and just like Candida albicans, Blasto lives in the digestive system of many, many different people, many healthy people, but it lives in balance and its kept in check by the beneficial bacteria. But when out of balance, Blasto can create a lot of problems for ...
The surfaces of massive corals of the genus Favia from Eilat, Red Sea, and from Heron Island, Great Barrier Reef, are covered by a layer of eukaryotic microorganisms. These microorganisms are embedded in the coral mucus and tissue. In the Gulf of Eilat, the prevalence of corals covered by patches of eukaryotic microorganisms was positively correlated with a decrease in water temperatures (from 25-28 degrees C in the summer to 20-23 degrees C in winter). Comparisons carried out using transmission and scanning electron microscopy showed morphological similarities between the microorganisms from the two geographically distant reefs. The microorganisms found on and in the tissues were approximately 5-15 microm in diameter, surrounded by scales in their cell wall, contained a nucleus, and included unique auto-florescent coccoid bodies of approximately 1 mum. Such morphological characters suggested that these microorganisms are stramenopile protists and in particular thraustochytrids. Molecular ...
Labile toxin producing enterotoxic E. coli (ETEC) were the commonest pathogen isolated from diarrheal stools of hospitalized children (21%) and adults (26%) in Singapore. Salmonellas ranked a close second in children (19%). Other bacterial pathogens were isolated from less than 5% of subjects. Blastocystis hominis was detected in 4.3% of diarrheal stools when a simple sedimentation technique was used. Cryptosporidium was not detected at all. An analysis of yeast counts in smears of diarrheal and non-diarrheal stools suggested they were etiologically associated with at least 6% of diarrhea in children and 19% in adults. Testing for rotaviruses by Latex agglutination and for adenovirus by electronmicroscopy showed an association with 6 per cent and 3 per cent diarrhea respectively. The study highlighted a need for: case control studies on ETEC and B. hominis; studies on the epidemiology of diarrhea by yeasts; establishing the true incidence of adenovirus diarrhea; studies on the prevalence and seasonality
Its well known among naturopaths and other alternative health practitioners that if one person in a household if infected by H pylori, other family or household members can also be infected.. In clinical practice I see this time and time again.. The trouble is that H pylori doesnt cause symptoms in everyone, so lets set the scene (this is a real life example from a family I worked with a few years ago).. Joyce has a whole bunch of digestive symptoms, but her husband, Colin, did not. Their daughter also had symptoms.. They all did a stool test like this one.. All three of them tested positive for H pylori, and both Joyce and her daughter tested positive for the Blastocystis hominis parasite.. This certainly isnt an isolated case - Ive seen H pylori in different household members on many occasions.. When more than one person in the household has H pylori, it can be passed from person-to-person.. Kissing, sexual contact and possibly even sharing eating and drinking utensils can lead to H ...
Routine Clinical Chemistry Tests. Albumin, Alkaline Phosphatase, ALT/SGPT, Ammonia, Amylase, AST/SGOT, Bilirubin, Blood Gases, BUN (Blood Urea Nitrogen), Calcium, Cholesterol, Cholinesterase, Creatinine, CK-MB, Electrolytes (Carbon Dioxide/Bicarbonate, Chloride, Potassium, Sodium), Ferritin, Follate/Folic Acid, Fructosamine, GGT, Glucose, HDL, Iron, LDH, Magnesium, Phosphorus, Potassium, Protein, Sodium, Triglycerides, Troponin, Uric Acid, Urinalysis, Vitamin B12.. Infectious Disease Tests. AIDS/HIV, Adenovirus, Aeromonads, Bartonella, Blastocystis Hominis, Campylobacter, Candida, Chancroid, Chlamydia, Clostridium, Coronavirus, Coxsackievirus, Cryptosporidium, Cyclospora, CMV, E. Coli, Echovirus, Encephalitis, Enterovirus, EBV, Giardia, Gonorrhea, Granuloma Inguinale, Hantavirus, H. Pylori, Hepatitis (A, B, C, D, E), Herpes Simplex Virus, Human Herpes Virus-6 (HHV-6), Influenza, Legionella, Lyme disease, Pymphogranuloma, Malaria, Measles, Meningitis, Mononucleosis, Microsporidium, Mononucleosis, ...
Nitazoxanide is a broad-spectrum antiparasitic and broad-spectrum antiviral drug that is used in medicine for the treatment of various helminthic, protozoal, and viral infections. It is indicated for the treatment of infection by Cryptosporidium parvum and Giardia lamblia in immunocompetent individuals and has been repurposed for the treatment of influenza. Nitazoxanide has also been shown to have in vitro antiparasitic activity and clinical treatment efficacy for infections caused by other protozoa and helminths; emerging evidence suggests that it possesses efficacy in treating a number of viral infections as well. Chemically, nitazoxanide is the prototype member of the thiazolides, a class of drugs which are synthetic nitrothiazolyl-salicylamide derivatives with antiparasitic and antiviral activity. Tizoxanide, an active metabolite of nitazoxanide in humans, is also an antiparasitic drug of the thiazolide class. Nitazoxanide is an effective first-line treatment for infection by Blastocystis ...
The first Apicomplexa protozoan was seen by Antonie van Leeuwenhoek, who in 1674 saw probably oocysts of Eimeria stiedae in the gall bladder of a rabbit. The first species of the phylum to be described, Gregarina ovata in earwigs intestines, was named by Dufour in 1828. He thought that they were a peculiar group related to the trematodes, at that time included in Vermes.[26] Since then, many more have been identified and named. During 1826-1850, 41 species and six genera of Apicomplexa were named. In 1951-1975, 1873 new species and 83 new genera were added.[26]. The older taxon Sporozoa, included in Protozoa, was created by Leuckart in 1879[27] and adopted by Bütschli in 1880.[28] Through history, it grouped with the current Apicomplexa many unrelated groups. For example, Kudo (1954) included in the Sporozoa species of the Ascetosporea (Rhizaria), Microsporidia (Fungi), Myxozoa (Animalia), and Helicosporidium (Chlorophyta), while Zierdt (1978) included the genus Blastocystis ...
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Toronto, ON) January 13, 2017- With lab results being the foundation of more than 70% of all health care decisions, time is always of the essence. As part of LifeLabs commitment to leading innovation and deliver quality information when patients and their health care providers need it most, we are pleased to announce a new partnership with Seegene Inc. to conduct verification studies of its molecular diagnostic technology. Testing for ova and parasites in stool samples is a highly manual process and one where the use of technology can improve quality and efficiency. The ability to deliver high quality microbiology results within rapid turnaround times is a critical differentiator in the field of diagnostics. Incorporating the Seegene Allplex panel and technology for parasites will help in the detection of the six most common parasites Giardia lamblia, Entamoeba histolytica, Cryptosporidium spp., Blastocystis hominis, Dientamoeba fragilis and Cyclospora cayetanensis. Achieved through the use of ...
The consortium between the colonial stramenopile Solenicola setigera and the centric chain-forming diatom Leptocylindrus mediterraneus is cosmopolitan throughout the world ocean yet rarely abundan... by fernando_gomez8953 in Browse > Science & Tech > Science > Earth & Life Sciences
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Purpose: Luminal A breast cancers have better prognosis than other molecular subtypes. Luminal A cancers may also be insensitive to adjuvant chemotherapy, although there is little high-level evidence to confirm this concept. The primary hypothesis in this formal prospective-retrospective analysis was to assess interaction between subtype (Luminal A vs. other) and treatment (chemotherapy vs. not) for the primary endpoint (10-year invasive disease-free survival) of a breast cancer trial randomizing women to adjuvant chemotherapy, analyzed in multivariate Cox proportional hazards models using the Wald interaction test.. Experimental Design: The Danish Breast Cancer Cooperative Group 77B clinical trial randomized 1,072 premenopausal women to no systematic treatment (control), levamisole, cyclophosphamide, or cyclophosphamide-methotrexate-fluorouracil arms. All arms included radiotherapy but no endocrine therapy. Researchers with no access to clinical data performed intrinsic subtype analysis on ...
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You should eat a lot of healthy fats, such as: coconut oil, avocado, olive oil, fish, nuts, seeds, probiotics… avoid birth control pills, metals, toxins from your tap water and you can start an alkaline diet that may be very good for your health.. Liver. If you want to get rid of the toxins you can use beets, milk thistle, dandelion root, burdock root, garlic, turmeric or castor oil. For cleansing the liver you should always use ornithine. You can also use some kind of tea, such as dandelion or tulsi.. Use olive oil to cleanse the liver. If you want to soften the gallstones you can try and eat tart apples for one week and also you can drink carrot juice. This way you will prevent them from dislodging. Afterwards drink a shot, not an alcoholic one, but olive oil shot with a little bit of lemon juice.. Kidney. If you want to flush the toxins out of your kidneys you should use ginger, vegetable glycerin, vitamin B6, horsetail and flor-essence tea. All of them will help you to flush out the toxins ...
G I D E O N GLOBAL INFECTIOUS DISEASES & EPIDEMIOLOGY NETWORK WHATS NEW ? Update 07.4 August 27 to September 2, 2007 NEW DISEASE ADDED Viliuisk encephalomyelitis EPIDEMIOLOGY ALL REPORTABLE DISEASES Spain Adenovirus Brazil, Italy AIDS Afghanistan, Dem. Rep. Congo, India Amebic colitis India Anthrax Mongolia, United States Ascariasis China, India Blastocystis Japan Clostridium difficile United States Cryptosporidiosis India Cysticercosis China, Mongolia Diphyllobothriasis Mongolia Ehrlichiosis - Hum. granulocyt. Israel Escherichia coli diarrhea Iran, Ireland, United States Gastroenteritis - viral Argentina, Brazil, Italy Giardiasis India Hantavirus - Old world China, Germany, Republic of Korea Hantavirus pulmonary syndrome Bolivia, Canada Hepatitis B Afghanistan Hepatitis C Afghanistan Hookworm China, India Influenza Indonesia, Worldwide Japanese encephalitis India, Nepal, Worldwide Leishmaniasis - cutaneous Morocco Leishmaniasis - visceral Portugal Leptospirosis Republic of Korea Malaria ...
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Blastocystis species Blastomycosis Blastomyces dermatitidis Bolivian hemorrhagic fever Machupo virus Botulism (and Infant ...
Blastocystis spp. intestinal direct microscopy of stool (PCR, antibody) • worldwide: one of the most common human parasites[1][ ... Table 2: Summary of treatments and efficacy for Blastocystis infection. *^ Amin OM (2002). "Seasonal prevalence of intestinal ... Blastocystis is now by far the most prevalent mono-infection in symptomatic patients in the United States [14] and was found ... Blastocystis is one of the most common intestinal protists of humans. ... A recent study showed that 100% of people from low ...
At the time, it was common practice to identify all Blastocystis from humans as Blastocystis hominis, while Blastocystis from ... CDC description of Blastocystis hominis Blastocystis Research Foundation Badbugs.org: Dientamoeba fragilis and Blastocystis ... Disregarding Blastocystis : In the early to mid-1990s, some US physicians suggested all findings of Blastocystis are ... Blastocystis cultured from patients who were sick and diagnosed with Blastocystis infection produced large, highly adhesive ...
Parasites, particularly protozoa (e.g., Cryptosporidium spp., Giardia spp., Entamoeba histolytica, Blastocystis spp., ...
Blastocystis is a single-celled protozoan which infects the large intestine. Physicians report that patients with infection ... Blastocystis was the most frequently identified protozoal infection found in patients in a 2006 Canadian study. 1918 flu ... Tan KS (2004). "Blastocystis in humans and animals: new insights using modern methodologies". Vet. Parasitol. 126 (1-2): 121-44 ... Zierdt CH (1991). "Blastocystis hominis--past and future". Clin. Microbiol. Rev. 4 (1): 61-79. PMC 358179 . PMID 2004348. ...
... infection by certain substypes of blastocystis hominis (blastocystosis). As of 2017, evidence indicates that blastocystis ... July 2017). "The role of Blastocystis sp. and Dientamoeba fragilis in irritable bowel syndrome: a systematic review and meta- ... "Blastocystis, an unrecognized parasite: an overview of pathogenesis and diagnosis". Ther Adv Infect Dis. 1 (5): 167-78. doi: ... suggesting that Blastocystis sp. infection is associated with a variety of gastrointestinal disorders, may play a significant ...
2007). "Finding of Blastocystis sp. in bivalves of the genus Donax". Rev. peru biol. 14 (2): 301-2. CS1 maint: Explicit use of ... included the genus Blastocystis (Stramenopiles). Some opisthokonts (e.g., Dermocystidium) were also thought to be sporozoan. ...
Blastocystis is one of the most common intestinal protists of humans. ... A recent study showed that 100% of people from low ... "Blastocystis: Resources for Health Professionals". United States Centers for Disease Control and Prevention. Retrieved 4 ... Nitazoxanide is an effective first-line treatment for infection by Blastocystis species and is indicated for the treatment of ... "Update on the pathogenic potential and treatment options for Blastocystis sp". Gut Pathog. 6: 17. doi:10.1186/1757-4749-6-17. ...
It includes Bicosoecida, Blastocystis and Labyrinthulida. It has also been described as containing Opalozoa, Bicoecia, and ...
Blastocystis Alexeev, 1911. *Bicosoecida Grasse, 1926 przywrócony przez Karpov, 1998. *Placidida Moriya i inni, 2002 ...
He described Blastocystis hominis and Entamoeba dispar. The latter species helped to explain why most people who appeared to be ...
... a 1996 in vitro study found Coptis chinensis extracts to be effective against the gastrointestinal parasite Blastocystis ... "In vitro response of Blastocystis hominis against traditional Chinese medicine". Journal of Ethnopharmacology. 55 (1): 35-42. ...
"Update on the pathogenic potential and treatment options for Blastocystis sp". Gut Pathogens. 6: 17. doi:10.1186/1757-4749-6-17 ... "Clinical efficacy of Saccharomyces boulardii or metronidazole in symptomatic children with Blastocystis hominis infection". ...
Regional and temporal variations in those illnesses follow those associated with infection with the protozoan Blastocystis. The ... Blastocystis, and asymptomatic infection". Parasit Vectors. 1 (1): 40. doi:10.1186/1756-3305-1-40. PMC 2627840 . PMID 18937874 ...
Additionally, Blastocystis species have been shown to have several subtypes that generate cysteine and aspartic protease ... Roberts T, Stark D, Harkness J, Ellis J (2014). "Update on the pathogenic potential and treatment options for Blastocystis sp ...
... and the stramenopile Blastocystis. The similarity between Nyctotherus and Blastocystis, which are only distantly related, is ... "Organelles in Blastocystis that Blur the Distinction between Mitochondria and Hydrogenosomes". Current Biology. 18 (8): 580-5. ...
The aqueous and ethanol extracts of the plant show antifungal properties, and anti parasitic activity against blastocystis ... March 2015). "Activity of medicinal plants from Ghana against the parasitic gut protist Blastocystis". J Ethnopharmacol. 174: ...
The genus Blastocystis, now in Stramenopiles, was originally classified as a yeast. Ellobiopsis, now in Alveolata, was ...
Blastocystis Alexeev, 1911. *Bicosoecida Grassé, 1926, emend. Karpov, 1998. *Placidida Moriya et al., 2002 ...
Aplanochytrium (Labyrinthulea) Blastocystis hominis (Blastocystea) Protoopalina pingi (Opalinea) Peronospora sparsa (Oomycetes ... Cavalier-Smith, 1997 (Slopalinida Patterson, 1985) Blastocystis Alexeev, 1911 Bicosoecida Grassé, 1926, emend. Karpov, 1998 ...
"Blastocystis: unravelling potential risk factors and clinical significance of a common but neglected parasite". Epidemiology ...
"The other intestinal protozoa: Enteric infections caused by Blastocystis hominis, Entamoeba coli, and Dientamoeba fragilis". ...
"Evaluation of DNA extraction kits for molecular diagnosis of human Blastocystis subtypes from fecal samples". Parasitology ...
"The association of Blastocystis hominis and Endolimax nana with diarrheal stools in Zambian school-age children". Digestive ...
Hoevers JD, Snowden KF (August 2005). "Analysis of the ITS region and partial ssu and lsu rRNA genes of Blastocystis and ...
Isolates of the human parasite Blastocystis hominis were observed to begin such a process within 4 to 6 days. Cells of the fish ...
Eighty patients with D-IBS and Blastocystis spp. infection were classified as cases and 80 with D-IBS without Blastocystis ... infection were classified as control. Cases were subdivided into D-IBS and Blastocystis sp. defined type 1 (subtype-specific ... Eighty patients with D-IBS and Blastocystis spp. infection were classified as cases and 80 with D-IBS without Blastocystis ... infection were classified as control. Cases were subdivided into D-IBS and Blastocystis sp. defined type 1 (subtype-specific ...
Blastocystis spp. infection, frequently asked questions, biology and publications. ... Blastocystis is a common microscopic organism that inhabits the intestine and is found throughout the world. A full ... understanding of the biology of Blastocystis and its relationship to other organisms is not clear, but is an active area of ...
Education and information about Blastocystis spp. and Blastocystis spp. infection biology. ... Blastocystis sp. is found worldwide. Efforts to characterize the geographic and host distribution of Blastocystis subtypes are ... Blastocystis has been detected in stool from humans and a wide range of non-human animals (e.g. canids, swine, primates, ... Blastocystis organisms isolated from humans have commonly been referred to as B. hominis. However, because of extensive genetic ...
... to anyone who is looking for a parasite treatment for Blastocystis Hominis, try looking up the website badbugs org, or the ... Blastocystis. Hello, to anyone who is looking for a parasite treatment for Blastocystis Hominis, try looking up the website ... Hello, to anyone who is looking for a parasite treatment for Blastocystis Hominis, try looking up the website badbugs org, or ... I was diagnosed with Blastocystis hominis. The stool test also revealed an abnormally low level of Secretory iga (sIgA). sIgA ...
So they called Blastocystis from humans Blastocystis hominis and gave different species names to Blastocystis from other ... CDC description of Blastocystis hominis Blastocystis Research Foundation Dientamoeba Fragilis and Blastocystis Hominis ... Blastocystis exhibits low host specificity, and many different species of Blastocystis can infect humans and by current ... Their proposal was to refer to Blastocystis from humans and animals as Blastocystis sp. subtype nn where nn is a number ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
... nelson/ce210a/Blastocystis/Blastocystis.htm. -A study done on Blastocystis hominis by a Berkeley student. Very helpful, ... Relationship of Blastocystis Species Among Eukaryotes. Relationship of Blastocystis species among eukaryotes as determined by ... 1996) Blastocystis hominis revisited. Clinical Microbiology Reviews. Oct: 563-584.. 6. Nelson, Kara L. Department of Civil & ... Differential Morphology of Protozoa Found in Stool Specimens of Humans: Ciliates, Coccidia, and Blastocystis ...
Blastocystis hominis Open pop-up dialog box Close Blastocystis hominis. Blastocystis hominis. Blastocystis is a microscopic ... Blastocystis hominis, also known as blastocystis spp or Blastocystis hominis infection, usually clears on its own. There are no ... Blastocystis hominis is common, and anyone can have the organism in his or her stools. You might be at higher risk if you ... Experts suspect that blastocystis gets into the digestive system when people eat contaminated food or are exposed to the stool ...
Recent studies indicate that the parasite is a species complex and humans are potentially hosts to nine Blastocystis... ... Blastocystis is an enteric protistan parasite of uncertain clinical relevance. ... Subtype analysis of Blastocystis isolates from Blastocystis cyst excreting patients. Am J Trop Med Hyg 2009;80:588-92.PubMed ... Blastocystis sp. subtype 5: a possibly zoonotic genotype. Parasitol Res 2007;101:1527-32.CrossRefPubMedGoogle Scholar ...
Scanning electron microscopy of the surface coat of Blastocystis hominis.. Zaman V1, Howe J, Ng M, Goh TK. ... Scanning electron microscopy of Blastocystis hominis showed that its outer coat has a fibrillar structure and individual ... It is postulated that breakdown of attached organisms may provide nutrients for Blastocystis. ...
Blastocystis ST3 and ST4 were the two most commonly found Blastocystis subtypes in Sweden, which is similar to results from ... The associations between Blastocystis and the bacterial microbiota found in this study could imply a link between Blastocystis ... Blastocystis sp. is a ubiquitous intestinal parasite with unsettled pathogenicity. Molecular studies of Blastocystis sp. have ... We found no statistical association between diarrhoea and Blastocystis sp., specific Blastocystis subtype or G. intestinalis. ...
Hemorrhagic Proctosigmoiditis and Blastocystis hominis Miguel Carrascosa, MD; Josefina Martinez, MD; Jose L. Perez-Castrillon, ... Hemorrhagic Proctosigmoiditis and Blastocystis hominis. Ann Intern Med. ;125:861. doi: 10.7326/0003-4819-125-10-199611150-00022 ...
Blastocystis spp. are ubiquitous anaerobic eukaryotes that have a unique phylogenetic origin but are widely present in various ... Blastocystis spp. are ubiquitous anaerobic eukaryotes that have a unique phylogenetic origin but are widely present in various ... Keywords: Pneumocystis, Microsporidia, Blastocystis, opportunistic eukaryotes, controversial pathogens Important Note: All ...
Abstract Blastocystis hominis, an intestinal organism of uncertain taxonomic position, has long been considered nonpathogenic. ... Blastocystis hominis: Pathogen or Fellow Traveler? * Edward K. Markell, Michael P. Udkow ... Blastocystis hominis, an intestinal organism of uncertain taxonomic position, has long been considered nonpathogenic. Some ...
Blastocystis hominis (B.hominis) is the most common unicellular protozoan parasite that is found in the human gastrointestinal ... Subtyping of Blastocystis isolates from symptomatic and asymptomatic cultures. Genomic DNA Extraction: The genomic DNA of ... Sample collection and axenization of Blastocystis hominis. B. hominis was isolated from stool samples of symptomatic as well as ... The knowledge and understanding in the association between Blastocystis hominis infection and colorectal cancer as well as the ...
Abstract The incidence of Blastocystis hominis in a healthy population was determined by fecal examination of 6,422 Japanese ... Epidemiologic Survey of Blastocystis hominis Infection in Japan * Authors: Noriyuki Horiki, Masataka Maruyama, Yoshiyuki Fujita ... The incidence of Blastocystis hominis in a healthy population was determined by fecal examination of 6,422 Japanese and 54 ...
Palabras clave: Blastocystis, Donax, Perú, Trujillo. Blastocystis hominis is one of the most common protozoan parasites of the ... It is the first report of Blastocystis in marine mollusks. The differentiation of new species of Blastocystis should be ... Keywords: Blastocystis, Donax, Peru, Trujillo. Resumen Aunque es detectado generalmente en seres humanos, los microorganismos ... In the present paper, we describe the finding of Blastocystis sp. in bivalves of the genus Donax. As a part of a study on human ...
Blastocystis hominis (B. hominis) is a microscopic parasite sometimes found in the stools of healthy people as well as in the ... However, Blastocystis hominis often appears with other organisms that may be the actual cause of the signs and symptoms ... Blood tests. Although there are no blood tests that can detect Blastocystis hominis, your doctor may order blood tests to look ... Many people who carry Blastocystis hominis have no signs or symptoms. Some people who have this parasite have diarrhea and ...
"Blastocystis Infections" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical ... This graph shows the total number of publications written about "Blastocystis Infections" by people in this website by year, ... Infections with organisms of the genus BLASTOCYSTIS. The species B. hominis is responsible for most infections. Parasitologic ... Below are the most recent publications written about "Blastocystis Infections" by people in Profiles. ...
We postulate that the alternative oxidase in Blastocystis is used to buffer transient oxygen fluctuations in the gut and that ... We postulate that the alternative oxidase in Blastocystis is used to buffer transient oxygen fluctuations in the gut and that ... Our results demonstrate that the Blastocystis cells themselves respire oxygen via this alternative oxidase thereby casting ... Our results demonstrate that the Blastocystis cells themselves respire oxygen via this alternative oxidase thereby casting ...
Population-level analysis of Blastocystis subtype prevalence and variation in the human gut microbiota ... Population-level analysis of Blastocystis subtype prevalence and variation in the human gut microbiota ... Population-level analysis of Blastocystis subtype prevalence and variation in the human gut microbiota ...
Infection with Blastocystis in humans has been associated with abdominal pain, diarrhea, constipation, fatigue, skin rash, and ... The variation in accounts has lead to disagreements concerning the role of Blastocystis in human disease, and the importance of ... A better understanding of the number of species of Blastocystis that can infect humans, along with realization of the ... The possibility that disagreement was caused by the emergence of particular pathogenic variants of Blastocystis is discussed, ...
Blastocystis hominis) (OPCA61246) , Recombinant Protein , Application: WB, ELISA , Species Reactivity: Blastocystis hominis , ... Youre reviewing: Elongation factor 1-alpha Recombinant Protein (Blastocystis hominis) (OPCA61246). How do you rate this ...
Steer H (2007) Blastocystis hominis and Colorectal Cancer. Ann R Coll Surg Engl 89:539CrossRefGoogle Scholar ... Scanlan PD (2012) Blastocystis past pit falls and future perspectives. Trends Parasitol 28:327-334CrossRefGoogle Scholar ... Blastocystis sp. Colorectal carcinoma Surface ultrastructure Protein profiles Zymography This is a preview of subscription ... Ragavan ND, Govind SK, Chye TT, Mahadeva S (2014) Phenotypic variation in Blastocystis sp. ST3. Parasites Vectors 7:404CrossRef ...
I think she has overlooked things like blastocystis hominis,and also her knowledge about diets seems limited... also her idea ... Re: So Called "Crohns disease" and Blastocystis ... R RONBN49 5years 9,329 * Re: So Called BIGBENNYmmmm 3years 6,219 ... So Called "Crohns disease" and Blastocystis Conn... R shrimpy123 6years 11,170 C * Wow! That s quite an angry rant. Many of us ... 11 yr.Boy, Blastocystis, courts involved, and Mend... shrimpy123 6years 10,005 * Oh my God. I think everyone should read that ...
  • infection were classified as 'cases' and 80 with D-IBS without Blastocystis spp. (aku.edu)
  • Which is a very remarkable observation, and maybe more interesting than the rest of the data, which I, by the way, find a bit difficult to follow (I expected to learn something about Blastocystis invasion, when I read the title of the abstract, but there is no data or information on invasiveness. (blastocystis.net)
  • Here, we first compared the prevalence of Blastocystis among 56 IBS patients 40 IBS with constipation IBS-C, 9 IBS with diarrhea IBS-D, 4 mixed IBS IBS-M and 3 unsubtyped IBS IBS-U according to the Rome III criteria and 56 control i.e. without any diagnosed chronic or acute gastrointestinal disorder subjects. (duhnnae.com)
  • Interestingly, in control subjects i.e. without any gastrointestinal disorder positive for Blastocystis, Faecalibacterium prausnitzii, which is known for its anti-inflammatory properties, was significantly decreased in men. (duhnnae.com)
  • A full understanding of the biology of Blastocystis and its relationship to other organisms is not clear, but is an active area of research. (cdc.gov)
  • Blastocystis organisms isolated from humans have commonly been referred to as B. hominis . (cdc.gov)
  • However, because of extensive genetic diversity (even among organisms isolated from humans) and low host specificity, the designation Blastocystis sp. (cdc.gov)
  • Blastocystis often appears with other organisms, so it's not known whether it causes disease. (mayoclinic.org)
  • It is postulated that breakdown of attached organisms may provide nutrients for Blastocystis. (nih.gov)
  • Infections with organisms of the genus BLASTOCYSTIS. (umassmed.edu)
  • Overall, this study provides an important window into the biology of Blastocystis, showcasing significant differences between STs that can guide future experimental investigations into differences in their virulence and clarifying the roles of these organisms in gut health and disease. (lshtm.ac.uk)
  • Unfortunately, we have not yet come up with a protocol on how to axenise Blastocystis cultures, i.e., get rid of metabolically active organisms other than Blastocystis in cultures while keeping Blastocystis alive and multiplying. (blastocystis.net)
  • A standard naming system for Blastocystis organisms from humans and animals has been proposed which names Blastocystis isolates according to the genetic identity of the Blastocystis organism rather than the host. (wikipedia.org)
  • Whether the associations between Blastocystis and the microbiota are resulting from the presence of Blastocystis, or are a prerequisite for colonization with Blastocystis, are interesting questions for further studies. (diva-portal.org)
  • However, the position of Blastocystis within the stramenopiles remains enigmatic. (wikipedia.org)
  • Arisue N, Hashimoto T, Yoshikawa H, Nakamura Y, Nakamura G, Nakamura F (2002) Phylogenetic position of Blastocystis hominis and of stramenopiles inferred from multiple molecular sequence data. (springer.com)
  • OBJECTIVE: Human gut microbiome studies are mainly bacteria- and archaea-oriented, overlooking the presence of single-cell eukaryotes such as Blastocystis, an enteric stramenopiles with worldwide distribution. (mdc-berlin.de)
  • Multiple stool samples from 460 children (53.9% male and 46.07% female) were collected and examined for the presence of Blastocystis hominis in Parasitology Laboratory of International Centre for Diarrhoeal Diseases Research, Bangladesh during the period of 9th January to 28th December, 2011. (bvsalud.org)
  • The finding of Blastocystis in faeces in mammals and birds has led to proposals of zoonotic potential and that these hosts may be the source of many human infections. (semanticscholar.org)
  • The possibility of zoonotic transmission to human from birds especially ostriches led us to investigate on the cross infectivity of Blastocystis sp. (biomedcentral.com)
  • The study further provides evidence for potential zoonotic transmission in ostrich farms as Blastocystis subtype 6 can infect rats and the same subtype have been previously reported in humans. (biomedcentral.com)
  • This study is the first to provide molecular-based evidence supporting the zoonotic potential of Blastocystis in dogs, possums and primates in a natural setting. (edu.au)
  • All the sequences of the Blastocystis-positive samples (KU051524-KU051536) were closely related to those from animals (pig, cattle, and chicken), indicating a zoonotic risk. (bvsalud.org)
  • Blastocystis is a common unicellular anaerobic eukaryote that inhabits the large intestine of many animals worldwide, including humans. (semanticscholar.org)
  • Blastocystis is a common gut eukaryote detected in humans and animals. (biomedcentral.com)
  • We aimed at assessing the impact of travel on Blastocystis carriage and seek associations between Blastocystis and the bacterial microbiota. (diva-portal.org)
  • The associations between Blastocystis and the bacterial microbiota found in this study could imply a link between Blastocystis and a healthy microbiota as well as with diets high in vegetables. (diva-portal.org)
  • This study investigates interactions between pathogenic human isolates belonging to Blastocystis subtype 7 (ST7) and bacterial representatives of the gut microbiota. (biomedcentral.com)
  • This study shows that particular isolates of Blastocystis ST7 cause changes in microbiota populations and potentially lead to an imbalance of the gut microbiota. (biomedcentral.com)
  • Blastocystis' role in IBS or IBD may thus be mediated by altering gut microbiota composition. (biomedcentral.com)
  • With these in mind, the current study explored the interactions between a particular ST of Blastocystis , ST7, and prokaryotic representatives of the gut microbiota. (biomedcentral.com)
  • Dentro de los millones de microorganismos que componen la microbiota intestinal, Clostridium difficile y Blastocystis pueden tener un efecto modulador en diferentes maneras. (urosario.edu.co)
  • De esta manera, Blastocystis puede favorecer el aumento de poblaciones de bacterias benéficas de la microbiota, al cumplir con su rol predatorio. (urosario.edu.co)
  • Lo anterior, ayudaría a esclarecer el estatus debatible de Blastocystis, inclinándose hacia la hipótesis de que podría ser un miembro benéfico para la microbiota intestinal. (urosario.edu.co)
  • El presente estudio también brinda una visión holística sobre la competencia que existe entre los miembros de la microbiota bajo un escenario de disbiosis, especialmente en la competencia por recursos entre Blastocystis y algunos hongos. (urosario.edu.co)
  • Within the thousands of microorganisms that make up the intestinal microbiota, Clostridium difficile and Blastocystis can have a modulatory effect in different ways. (urosario.edu.co)
  • Hence, Blastocystis can favor the increasement of populations of beneficial bacteria of the microbiota, by performing its predatory role. (urosario.edu.co)
  • Thus, our results would help clarify the debatable status of Blastocystis, leaning towards the hypothesis that it could be a beneficial member of the intestinal microbiota. (urosario.edu.co)
  • The present study can also provide a holistic insight into the competition that exists among members of the microbiota under a scenario of dysbiosis, especially in the competition for resources between Blastocystis and some fungi. (urosario.edu.co)
  • Within the FGFP cohort, out of 69 previously identified gut microbiota covariates, only age was associated with Blastocystis subtype carrier status. (mdc-berlin.de)
  • CONCLUSIONS: These results emphasise the role of Blastocystis as a common constituent of the healthy gut microbiota. (mdc-berlin.de)
  • We show its prevalence is reduced in patients with active IBD and demonstrate that subtype characterisation is essential for assessing the relationship between Blastocystis, microbiota profile and host health. (mdc-berlin.de)
  • Blastocystis is a prevalent enteric protist that infects a variety of vertebrates. (biomedcentral.com)
  • Blastocystis is a common protist isolated in humans and many animals. (nih.gov)
  • Blastocystis is a common enteric protist colonizing probably more than 1 billion people with a large variety of non-human hosts. (urosario.edu.co)
  • Scanning electron microscopy of the surface coat of Blastocystis hominis. (nih.gov)
  • Cassidy MF, Stenzel DJ, Boreham PFL (1994) Electron microscopy of surface structures of Blastocystis sp. (springer.com)
  • Blastocystis may be easily missed on microscopy due to its small size and irregular shape, sometimes being misidentified as yeast, lipid, or even an artefact. (waterpathogens.org)
  • Reagents are available commercially that facilitate identification of Blastocystis by immunofluorescence microscopy. (waterpathogens.org)
  • A total of 100 Blastocystis isolates by microscopy and culture methods were obtained. (semanticscholar.org)
  • Clark CG, van der Giezen M, Alfellani MA, Stensvold CR (2013) Recent developments in Blastocystis research. (springermedizin.de)
  • Acute urticaria associated with amoeboid forms of Blastocystis sp. (springer.com)
  • In vitro and animal studies shed new light on the pathobiology of Blastocystis . (springer.com)
  • Comparative Study of Wheatley's Trichrome Stain and In-vitro Culture against PCR Assay for the Diagnosis of Blastocystis sp. (semanticscholar.org)
  • Stool samples collected from 125 children, within a period of 7 months, were examined using direct wet mount and trichrome stain in comparison to in vitro culture on Jones medium (for detecting Blastocystis spp. (springermedizin.de)
  • In vitro assays demonstrate that Blastocystis -induced host responses are able to decrease Bifidobacterium counts. (biomedcentral.com)
  • 2. Leelayoova S, Taamasri P, Rangsin R, Naaglor T, Thathaisong U, Mungthin M. In-vitro Cultivation: A Sensitive Method for Detecting Blastocystis hominis . (oregonstate.edu)
  • In vitro effect of some Egyptian herbal extracts against Blastocystis hominis . (cfsremission.com)
  • A few traditional Chinese medicinal herbs have also been examined for in vitro activity against Blastocystis ( Brucea javanica and Coptis chinensis ). (cfsremission.com)
  • In vitro propagation followed by PCR, and a PCR-based method capable of the direct detection of Blastocystis in faeces were utilized to detect Blastocystis from various hosts in Australia, including primates and their handlers from the Perth Zoo. (edu.au)
  • PCR-based detection directly from faeces was shown to be more sensitive compared with in vitro culture for the detection of Blastocystis. (edu.au)
  • Establishment of Blastocystis hominis in-vitro Culture Using Fecal Samples from Infants in Slum Area of Mirpur, Dhaka, Bangladesh. (bvsalud.org)
  • Blastocystis hominis: axenic cultivation. (nih.gov)
  • Researchers from Wroclaw University of Environmental and Life Sciences have published a study comparing different culture media for Blastocystis, reporting that "In this article, we compared eight liquid media for cultivation of Blastocystis spp. (bhomcenter.org)
  • van Hellemond JJ, Molhoek N, Koelewijn R, Wismans PJ, van Genderen PJJ (2013) Is paromomycin the drug of choice for eradication of Blastocystis in adults? (swabid.nl)
  • 2010) Eradication of Blastocystis carriage with antimicrobials: reality or delusion? (swabid.nl)
  • In the present paper, we describe the detection of Blastocystis sp. (scielo.org.pe)
  • Meanwhile, PCR-based detection is currently state-of-the-art, and analysis of Blastocystis nuclear ribosomal DNA enables subtype identification. (waterpathogens.org)
  • There are several methods for the detection of Blastocystis . (biomedcentral.com)
  • PCR and DNA sequencing are currently the diagnostic methods of choice for detection of Blastocystis spp. (cdc.gov)
  • Concentration and purity were measured with Nano-Drop, then PCR and sequencing were conducted for detection of Blastocystis spp. (cdc.gov)
  • Genetic diversity of blastocystis in livestock and zoo animals. (semanticscholar.org)
  • Genetic variability of Blastocystis sp. (semanticscholar.org)
  • Here we investigated the incidence and genetic diversity of Blastocystis in a US population and also the possibility of Blastocystis human-human transmission between healthy individuals using family units (N = 50) living in Boulder, Colorado as our sample-set. (ucc.ie)
  • All stool samples were cultivated, and 550-bp fragments of the small subunit ribosomal RNA gene was amplified from Blastocystis -positive cultures. (springermedizin.de)
  • Cytologic diagnosis of Blastocystis hominis in peritoneal fluid: a case report. (springer.com)
  • Elghareeb AS, Younis MS, El Fakahany AF, Nagaty IM, Nagib MM (2015) Laboratory diagnosis of Blastocystis spp. (springermedizin.de)
  • Laboratory diagnosis of Blastocystis spp. (blastocystis.net)
  • To our knowledge, this is the first study that demonstrates DNA extraction from DSSFP is more sensitive and effective than the FS method for diagnosis of Blastocystis spp. (cdc.gov)
  • From molecular epidemiology studies and traditional epidemiology results from family members and small communities, the fecal-oral route is considered the main transmission route for Blastocystis hominis infections in humans. (scielo.org.pe)
  • Molecular characterization of Blastocystis from pigs in Shaanxi province of China. (semanticscholar.org)
  • Molecular Identification and Subtype Analysis of Blastocystis . (blastocystis.net)
  • Molecular epidemiology of Blastocystis sp. (semanticscholar.org)
  • Further studies implementing high-resolution molecular markers are necessary to understand the dynamics of Blastocystis transmission and the role of this Stramenopila in health and disease. (urosario.edu.co)
  • Most commonly, blastocystis simply lives in a person's digestive tract without causing harm. (mayoclinic.org)
  • Although commonly detected in humans, microorganisms identified as Blastocystis have also been isolated from a wide range of animals, such as primates, pigs, cattle, birds, amphibians and, less frequently , rodents and insects. (scielo.org.pe)
  • Blastocystis carriage was positively associated with high bacterial genus richness, and negatively correlated to the Bacteroides-driven enterotype. (diva-portal.org)
  • The samples were submitted to DNA extraction, Real Time PCR and sequencing using Blastocystis-specific primers targeting the small subunit rRNA gene for ST identification. (urosario.edu.co)
  • Conclusions: The high rate of Blastocystis subtype persistence found during travel indicates that long-term carriage of Blastocystis is common. (diva-portal.org)
  • Thus, we investigated the possible impact of Blastocystis carriage on the enteric bacterial community through quantification of 8 major bacterial groups from the enteric flora. (duhnnae.com)
  • Our results demonstrate that the Blastocystis cells themselves respire oxygen via this alternative oxidase thereby casting doubt on its strict anaerobic nature. (frontiersin.org)
  • Although Blastocystis has a global distribution, there is dearth of information relating to its prevalence and diversity in many human populations. (ucc.ie)