Blastocyst Inner Cell Mass: The cluster of cells inside a blastocyst. These cells give rise to the embryonic disc and eventual embryo proper. They are pluripotent EMBRYONIC STEM CELLS capable of yielding many but not all cell types in a developing organism.Blastocyst: A post-MORULA preimplantation mammalian embryo that develops from a 32-cell stage into a fluid-filled hollow ball of over a hundred cells. A blastocyst has two distinctive tissues. The outer layer of trophoblasts gives rise to extra-embryonic tissues. The inner cell mass gives rise to the embryonic disc and eventual embryo proper.Embryonic Development: Morphological and physiological development of EMBRYOS.Morula: An early embryo that is a compact mass of about 16 BLASTOMERES. It resembles a cluster of mulberries with two types of cells, outer cells and inner cells. Morula is the stage before BLASTULA in non-mammalian animals or a BLASTOCYST in mammals.Embryo Culture Techniques: The technique of maintaining or growing mammalian EMBRYOS in vitro. This method offers an opportunity to observe EMBRYONIC DEVELOPMENT; METABOLISM; and susceptibility to TERATOGENS.Trophoblasts: Cells lining the outside of the BLASTOCYST. After binding to the ENDOMETRIUM, trophoblasts develop into two distinct layers, an inner layer of mononuclear cytotrophoblasts and an outer layer of continuous multinuclear cytoplasm, the syncytiotrophoblasts, which form the early fetal-maternal interface (PLACENTA).Embryonic and Fetal Development: Morphological and physiological development of EMBRYOS or FETUSES.Embryo, Mammalian: The entity of a developing mammal (MAMMALS), generally from the cleavage of a ZYGOTE to the end of embryonic differentiation of basic structures. For the human embryo, this represents the first two months of intrauterine development preceding the stages of the FETUS.Embryo Implantation: Endometrial implantation of EMBRYO, MAMMALIAN at the BLASTOCYST stage.Blastomeres: Undifferentiated cells resulting from cleavage of a fertilized egg (ZYGOTE). Inside the intact ZONA PELLUCIDA, each cleavage yields two blastomeres of about half size of the parent cell. Up to the 8-cell stage, all of the blastomeres are totipotent. The 16-cell MORULA contains outer cells and inner cells.Octamer Transcription Factor-3: An octamer transcription factor that is expressed primarily in totipotent embryonic STEM CELLS and GERM CELLS and is down-regulated during CELL DIFFERENTIATION.Cleavage Stage, Ovum: The earliest developmental stage of a fertilized ovum (ZYGOTE) during which there are several mitotic divisions within the ZONA PELLUCIDA. Each cleavage or segmentation yields two BLASTOMERES of about half size of the parent cell. This cleavage stage generally covers the period up to 16-cell MORULA.Embryo Transfer: The transfer of mammalian embryos from an in vivo or in vitro environment to a suitable host to improve pregnancy or gestational outcome in human or animal. In human fertility treatment programs, preimplantation embryos ranging from the 4-cell stage to the blastocyst stage are transferred to the uterine cavity between 3-5 days after FERTILIZATION IN VITRO.Fertilization in Vitro: An assisted reproductive technique that includes the direct handling and manipulation of oocytes and sperm to achieve fertilization in vitro.Endoderm: The inner of the three germ layers of an embryo.Ectoderm: The outer of the three germ layers of an embryo.Pregnancy: The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.Zygote: The fertilized OVUM resulting from the fusion of a male and a female gamete.Chimera: An individual that contains cell populations derived from different zygotes.Embryonic Stem Cells: Cells derived from the BLASTOCYST INNER CELL MASS which forms before implantation in the uterine wall. They retain the ability to divide, proliferate and provide progenitor cells that can differentiate into specialized cells.Gene Expression Regulation, Developmental: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action during the developmental stages of an organism.Ectogenesis: Embryonic and fetal development that takes place in an artificial environment in vitro.Germ Layers: The three primary germinal layers (ECTODERM; ENDODERM; and MESODERM) developed during GASTRULATION that provide tissues and body plan of a mature organism. They derive from two early layers, hypoblast and epiblast.Parthenogenesis: A unisexual reproduction without the fusion of a male and a female gamete (FERTILIZATION). In parthenogenesis, an individual is formed from an unfertilized OVUM that did not complete MEIOSIS. Parthenogenesis occurs in nature and can be artificially induced.Nuclear Transfer Techniques: Methods of implanting a CELL NUCLEUS from a donor cell into an enucleated acceptor cell.GATA6 Transcription Factor: A GATA transcription factor that is expressed predominately in SMOOTH MUSCLE CELLS and regulates vascular smooth muscle CELL DIFFERENTIATION.Culture Techniques: Methods of maintaining or growing biological materials in controlled laboratory conditions. These include the cultures of CELLS; TISSUES; organs; or embryo in vitro. Both animal and plant tissues may be cultured by a variety of methods. Cultures may derive from normal or abnormal tissues, and consist of a single cell type or mixed cell types.Embryo Implantation, Delayed: Delay in the attachment and implantation of BLASTOCYST to the uterine ENDOMETRIUM. The blastocyst remains unattached beyond the normal duration thus delaying embryonic development.Cloning, Organism: The formation of one or more genetically identical organisms derived by vegetative reproduction from a single cell. The source nuclear material can be embryo-derived, fetus-derived, or taken from an adult somatic cell.Cell Lineage: The developmental history of specific differentiated cell types as traced back to the original STEM CELLS in the embryo.Cell Count: The number of CELLS of a specific kind, usually measured per unit volume or area of sample.Ear, Inner: The essential part of the hearing organ consists of two labyrinthine compartments: the bony labyrinthine and the membranous labyrinth. The bony labyrinth is a complex of three interconnecting cavities or spaces (COCHLEA; VESTIBULAR LABYRINTH; and SEMICIRCULAR CANALS) in the TEMPORAL BONE. Within the bony labyrinth lies the membranous labyrinth which is a complex of sacs and tubules (COCHLEAR DUCT; SACCULE AND UTRICLE; and SEMICIRCULAR DUCTS) forming a continuous space enclosed by EPITHELIUM and connective tissue. These spaces are filled with LABYRINTHINE FLUIDS of various compositions.Oocytes: Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM).Totipotent Stem Cells: Single cells that have the potential to form an entire organism. They have the capacity to specialize into extraembryonic membranes and tissues, the embryo, and all postembryonic tissues and organs. (Stem Cells: A Primer [Internet]. Bethesda (MD): National Institutes of Health (US); 2000 May [cited 2002 Apr 5]. Available from: http://www.nih.gov/news/stemcell/primer.htm)Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Fetal Viability: The potential of the FETUS to survive outside the UTERUS after birth, natural or induced. Fetal viability depends largely on the FETAL ORGAN MATURITY, and environmental conditions.HMGB Proteins: A family of sequence-related proteins similar to HMGB1 PROTEIN that contains specific HMG-BOX DOMAINS.Fibroblast Growth Factor 4: A HEPARIN binding fibroblast growth factor that may play a role in LIMB BUDS development.Cryopreservation: Preservation of cells, tissues, organs, or embryos by freezing. In histological preparations, cryopreservation or cryofixation is used to maintain the existing form, structure, and chemical composition of all the constituent elements of the specimens.Pluripotent Stem Cells: Cells that can give rise to cells of the three different GERM LAYERS.Zona Pellucida: A tough transparent membrane surrounding the OVUM. It is penetrated by the sperm during FERTILIZATION.Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Mice, Inbred Strains: Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.SOXF Transcription Factors: A subclass of closely-related SOX transcription factors. Members of this subclass are expressed in VASCULAR ENDOTHELIAL CELLS and may play a role in vasculogenesis.Mice, Inbred CBACell Aggregation: The phenomenon by which dissociated cells intermixed in vitro tend to group themselves with cells of their own type.Psittaciformes: An order of BIRDS comprised of several families and more than 300 species. It includes COCKATOOS; PARROTS; PARAKEETS; macaws; and BUDGERIGARS.Genetic Complementation Test: A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.Handling (Psychology): Physical manipulation of animals and humans to induce a behavioral or other psychological reaction. In experimental psychology, the animal is handled to induce a stress situation or to study the effects of "gentling" or "mothering".Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Extraembryonic Membranes: The thin layers of tissue that surround the developing embryo. There are four extra-embryonic membranes commonly found in VERTEBRATES, such as REPTILES; BIRDS; and MAMMALS. They are the YOLK SAC, the ALLANTOIS, the AMNION, and the CHORION. These membranes provide protection and means to transport nutrients and wastes.Zebrafish: An exotic species of the family CYPRINIDAE, originally from Asia, that has been introduced in North America. They are used in embryological studies and to study the effects of certain chemicals on development.

Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization. (1/83)

BACKGROUND: Embryonic stem cells (ESC) are pluripotent cells obtained from the inner cell mass (ICM) of blastocysts derived from in vitro culture associated with reproductive endocrinology therapy. Human ESCs are regarded as highly significant since they retain the capacity to differentiate into any of approximately 200 unique cell types. Human ESC research is controversial because to acquire such cells, the ICM of human blastocysts must be manipulated in a way that renders embryos nonviable and unsuitable for transfer in utero. Techniques to yield competent ESCs with conservation of source blastocysts would satisfy many objections against ESC research, but at present such approaches remain largely untested. RESULTS AND DISCUSSION: We contrast experimental culture of single blastomeres obtained by 1) non-destructive biopsy of embryos destined for transfer, and 2) isolation of karyotypically normal blastomeres from disaggregated ("dead") embryos considered unsuitable for transfer, and evaluate these approaches with regard to production of ESCs. Pluripotency was confirmed by morphological criteria and by quantification of divergent homeodomain proteins specific to undifferentiated cell development. Following ESC isolation and identification, assessment was conducted according to a novel ESC grading system, also proposed here. CONCLUSION: The role of reproductive endocrinology in ESC research remains paramount. In this report, we hypothesize new and expand on existing strategies having the potential to enhance human ESC isolation, identification and in vitro maintenance.  (+info)

Direct derivation of neural rosettes from cloned bovine blastocysts: a model of early neurulation events and neural crest specification in vitro. (2/83)

Embryonic stem cells differentiate into neuroectodermal cells under specific culture conditions. In primates, these cells are organized into rosettes expressing Pax6 and Sox1 and are responsive to inductive signals such as Sonic hedgehog (Shh) and retinoic acid. However, direct derivation of organized neuroectoderm in vitro from preimplantation mammalian embryos has never been reported. Here, we show that bovine inner cell masses from nuclear transfer and fertilized embryos, grown on feeders in serum-free medium, form polarized rosette structures expressing nestin, Pax6, Pax7, Sox1, and Otx2 and exhibiting interkinetic nuclear migration activity and cell junction distribution as in the developing neural tube. After in vitro expansion, neural rosettes give rise to p75-positive neural crest precursor cell lines capable of long-term proliferation and differentiation in autonomic and sensory peripheral neurons, glial cells, melanocytes, smooth muscle cells, and chondrocytes, recapitulating in vitro the unique plasticity of the neural crest lineage. Challenging the rosette dorsal fate by early exposure to Shh induces the expression of ventral markers Isl1, Nkx2.2, and Nkx6.1 and differentiation of mature astrocytes and neurons of central nervous system ventral identity, demonstrating appropriate response to inductive signals. All together, these findings indicate that neural rosettes directly derived from cloned and fertilized bovine embryos represent an in vitro model of early neural specification and differentiation events. Moreover, this study provides a source of highly proliferative neural crest precursor cell lines of wide differentiation potential for cell therapy and tissue engineering applications.  (+info)

Aggregating embryonic but not somatic nuclear transfer embryos increases cloning efficiency in cattle. (3/83)

Our objectives were to compare the cellular and molecular effects of aggregating bovine embryonic vs. somatic cell nuclear transfer (ECNT vs. SCNT) embryos and to determine whether aggregation can improve cattle cloning efficiency. We reconstructed cloned embryos from: 1) morula-derived blastomeres, 2) six adult male ear skin fibroblast lines, 3) one fetal female lung fibroblast line (BFF), and 4) two transgenic clonal strains derived from BFF. Embryos were cultured either singularly (1X) or as aggregates of three (3X). In vitro-fertilized (IVF) 1X and 3X embryos served as controls. After aggregation, the in vitro development of ECNT but not that of SCNT or IVF embryos was strongly compromised. The inner cell mass (ICM), total cell (TC) numbers, and ICM:TC ratios significantly increased for all the aggregates. The relative concentration of the key embryonic transcript POU5F1 (or OCT4) did not correlate with these increases, remaining unchanged in the ECNT and IVF aggregates and decreasing significantly in the SCNT aggregates. Overall, the IVF and 3X ECNT but not the 1X ECNT embryos had significantly higher relative POU5F1 levels than the SCNT embryos. High POU5F1 levels correlated with high in vivo survival, while no such correlation was noted for the ICM:TC ratios. Development to weaning was more than doubled in the ECNT aggregates (10/51 or 20% vs. 7/85 or 8% for 3X vs. 1X, respectively; P < 0.05). In contrast, the SCNT and IVF controls showed no improvement in survival. These data reveal striking biological differences between embryonic and somatic clones in response to aggregation.  (+info)

Murine inner cell mass-derived lineages depend on Sall4 function. (4/83)

Sall4 is a mammalian Spalt transcription factor expressed by cells of the early embryo and germ cells, an expression pattern similar to that of both Oct4 and Sox2, which play essential roles during early murine development. We show that the activity of Sall4 is cell-autonomously required for the development of the epiblast and primitive endoderm from the inner cell mass. Furthermore, no embryonic or extraembryonic endoderm stem cell lines could be established from Sall4-deficient blastocysts. In contrast, neither the development of the trophoblast lineage nor the ability to generate trophoblast cell lines from murine blastocysts was impaired in the absence of Sall4. These data establish Sall4 as an essential transcription factor required for the early development of inner cell mass-derived cell lineages.  (+info)

Enhanced development of porcine embryos cloned from bone marrow mesenchymal stem cells. (5/83)

In the present study, we have characterized an isolated population of porcine bone marrow mesenchymal stem cells (MSCs) for multilineage commitment and compared the developmental potential of cloned embryos with porcine MSCs and fetal fibroblasts (FFs). MSCs exhibited robust alkaline phosphatase activity and later transformed into mineralized nodules following osteoinduction. Furthermore, MSCs underwent adipogenic and chondrogenic differentiation by producing lipid droplets and proteoglycans, respectively. Primary cultures of FFs from a female fetus at ~30 day of gestation were established. Donor cells at 3-4 passage were employed for nuclear transfer (NT). Cell cycle analysis showed that the majority of MSCs in confluence were in the G0/G1 stage. Cumulus-oocyte complexes were matured and fertilized in vitro (IVF) as control. The cleavage rate was significantly (P<0.05) higher in IVF than in NT embryos with MSCs and FFs (84.54.6% vs. 52.25.4% and 50.85.2%, respectively). However, blastocyst rates in IVF and NT embryos derived from MSCs (20.62.5% and 18.43.0%) did not differ, but were significantly (P<0.05) higher than NT derived from FFs (9.52.1%). Total cell number and the ratio of ICM to total cells among blastocysts cloned from MSCs (34.45.2 and 0.380.08, respectively) were significantly (P<0.05) higher than those from FFs (22.65.5 and 0.180.12, respectively). Proportions of TUNEL positive cells in NT embryos from FFs (7.31.8%) were significantly (P<0.05) higher than in MSCs (4.61.3%) and IVF (2.50.9%). The results clearly demonstrate that multipotent bone marrow MSCs have a greater potential as donor cells than FFs in achieving enhanced production of cloned porcine embryos.  (+info)

Histone arginine methylation regulates pluripotency in the early mouse embryo. (6/83)

It has been generally accepted that the mammalian embryo starts its development with all cells identical, and only when inside and outside cells form do differences between cells first emerge. However, recent findings show that cells in the mouse embryo can differ in their developmental fate and potency as early as the four-cell stage. These differences depend on the orientation and order of the cleavage divisions that generated them. Because epigenetic marks are suggested to be involved in sustaining pluripotency, we considered that such developmental properties might be achieved through epigenetic mechanisms. Here we show that modification of histone H3, through the methylation of specific arginine residues, is correlated with cell fate and potency. Levels of H3 methylation at specific arginine residues are maximal in four-cell blastomeres that will contribute to the inner cell mass (ICM) and polar trophectoderm and undertake full development when combined together in chimaeras. Arginine methylation of H3 is minimal in cells whose progeny contributes more to the mural trophectoderm and that show compromised development when combined in chimaeras. This suggests that higher levels of H3 arginine methylation predispose blastomeres to contribute to the pluripotent cells of the ICM. We confirm this prediction by overexpressing the H3-specific arginine methyltransferase CARM1 in individual blastomeres and show that this directs their progeny to the ICM and results in a dramatic upregulation of Nanog and Sox2. Thus, our results identify specific histone modifications as the earliest known epigenetic marker contributing to development of ICM and show that manipulation of epigenetic information influences cell fate determination.  (+info)

Foetal fibroblasts introduced to cleaving mouse embryos contribute to full-term development. (7/83)

Foetal fibroblasts (FFs) labelled with vital fluorescent dye were microsurgically introduced into eight-cell mouse embryos, three cells to each embryo. FFs were first identified in the inner cell mass (ICM) in about one-third of embryos, whereas in three quarters of embryos FFs were located among trophoblast cells. Some elimination of FFs from trophoblast occurred later on. Eventually, in blastocysts' outgrowths, an equally high contribution from FFs progeny (60%) was found in both ICM and trophoblast. Three days after manipulation, FFs resumed proliferation in vitro. More than three FFs were found in 46.2% of embryos on day 4. On the 7th day in vitro in 70% of embryos more than 12 FFs were found, proving at least three cell divisions. To study postimplantation development, the embryos with FFs were transferred to pseudopregnant recipients a day after manipulation. After implantation, FFs were identified by electrophoresis for isozymes of glucose phosphate isomerase (GPI). A single 11-day embryo delayed to day 8 proved chimeric by expressing both donor isozyme GPI-1B and recipient GPI-1A. Similar chimerism was found in the extraembryonic lineage of 11% of embryos by day 12. Starting from day 11 onwards, in 32% of normal embryos and in 57% of foetal membranes, hybrid GPI-1AB isozyme, as well as recipient isozyme, was present. Hybrid GPI-1AB can only be produced in hybrid cells derived by cell fusion, therefore, we suggest that during postimplantation development, FFs are rescued by fusion with recipient cells. In the mice born, hybrid isozyme was found in several tissues, including brain, lung, gut and kidney. We conclude that somatic cells (FFs) can proliferate in early embryonic environment until early postimplantation stages. Foetuses and the mice born are chimeras between recipient cells and hybrid cells with contributions from the donor FFs. Transdifferentiation as opposed to reprogramming by cell fusion can be considered as underlying cellular processes in these chimeras.  (+info)

Blastocyst axis is specified independently of early cell lineage but aligns with the ZP shape. (8/83)

The mechanisms controlling the establishment of the embryonic-abembryonic (E-Ab) axis of the mammalian blastocyst are controversial. We used in vitro time-lapse imaging and in vivo lineage labeling to provide evidence that the E-Ab axis of the mouse blastocyst is generated independently of early cell lineage. Rather, both the boundary between two-cell blastomeres and the E-Ab axis of the blastocyst align relative to the ellipsoidal shape of the zona pellucida (ZP), an extraembryonic structure. Lack of correlation between cell lineage and the E-Ab axis can be explained by the rotation of the embryo within the ZP.  (+info)

UPDATE: ICM is trying to prevent the hot leading man of HBOs True Blood from leaving the agency. Theyre re-meeting, an insider tells me. But Ive learned Stephen Moyer has already signalled his intent to exit and considers himself a free agent and is about to start meeting with other tenpe...
Does the answer to music retailings collapse lie within the computer game industry? Global music revenues suffered a 10th year of decline in 2009, with sales and performance rights falling to $17 billion. In contrast, revenue from the computer game industrys sale of portable and console hardware, software, and accessories rose from $7.98 billion in 2000 to $20.2 billion in 2009 in the U.S. alone. The music industry may find inspiration in the game industry, where the prevailing Internet-driven
Up until at least 7 1/4 days p.c., the orientation of all axes in the mouse conceptus, embryonic axis included, is directly correlated with two orientations of the 82 h blastocyst within the lumen. These two are: the almost horizontal position of the blastocysts inner cell mass-abembryonic pole axi …
The inner cell mass (ICM) is the part of the blastocyst that is fated to become the embryo, amnion, and yolk sac. In contrast, the trophectoderm cells of the blastocyst will contribute to extra-embryonic tissues, such as the placenta and umbilical cord. Cells of the ICM are pluripotent, meaning they can give rise to all the cell lineages (ectoderm, endoderm, and mesoderm) found in the body. The ICM is, thus, a source of embryonic stem cells.. ...
Sigma-Aldrich offers abstracts and full-text articles by [Duancheng Wen, Nestor Saiz, Zev Rosenwaks, Anna-Katerina Hadjantonakis, Shahin Rafii].
Transcription factor control of TE/ICM segregation. TE and ICM lineage segregation is controlled by a small group of transcription factors. Specifically, Cdx2 is required for TE development, while the pluripotency markers octamer 3/4 (Oct4), Nanog, and SRY-box containing gene 2 (Sox2) are involved in establishing the ICM fate. In the mouse, Cdx2 is expressed at varying levels in all blastomeres starting at the eight-cell stage, but it becomes restricted to outside, future TE cells, prior to blastocyst formation (Figure 1) (72, 73). This variation in Cdx2 levels between individual blastomeres at the eight-cell stage may be a result of differences in the order and orientation of the cleavage divisions leading up to this stage (71). Embryos missing Cdx2 do form blastocysts initially, but the TE in these embryos loses its epithelial integrity and cannot differentiate further, resulting in death around the time of implantation (74). Oct4, Nanog, and Sox2 have expression patterns that are ...
Immunosurgery is a useful technique for the isolation of inner cell masses from murine blastocysts. Conventionally, rabbit antisera made ad hoc against murine splenic or fetal cells or fibroblasts have been used as antibody sources. We investigated the feasibility of using commercially available rabbit antiserum to murine erythrocytes (anti-RBC) and compared it with rabbit antiserum generated ad hoc to murine L-cells (anti-L-cell). Our results indicate that anti-RBC is at least as effective as anti-L-cell serum for the immunosurgical isolation of inner cell masses, which became either miniblastocysts (later forming outgrowths) or embryoid bodies (undergoing ectoderm-endodermlike differentiation within 48 h). Because anti-RBC is commercially available, the technical modification described herein increases the accessibility of the immunosurgical protocol for the isolation of murine inner cell masses.
Researchers supported the use of the portal vein as a site for islet transplantation, but noted that there are issues with injecting encapsulated cells into the liver. Finally, the research team suggested decreasing capsule size to nano-scale and combining PEGylation coating of capsules with a layer of poly(ethylene) glycol molecules with low-dose immunosuppression to improve engraftment and long-term function.. What about stem cells?. Human embryonic stem cells emerge five to seven days into an embryos development, when the embryo is a hollow sphere. The sphere consists of an outer layer of cells that goes on to form the placenta, and an inner cluster of cells known as the inner cell mass that goes on to form all of the tissues of the body. At this stage, the embryo is known as a blastocyst. Embryonic stem cells arise from the inner cell mass. (Picture shows a five-day-old embryo, known as a blastocyst. The dark patch inside the blastocyst is the inner cell mass, from which embryonic stem ...
So, yes. I do in fact look at the inner cell mass and the trophoblast and say that it is a human being. I have read several intelligent and articulate people who disagree with that belief, understood their arguments, and walked away from the experience still not convinced that the embryo is anything other than a human being. Many people have made clever arguments that it is not, but I have yet to hear much in the way of what exactly constitutes a human being if we disqualify the embryo ...
MIT and Harvard researchers have developed a novel way to measure the instantaneous growth rate of a single cell. The methodology builds on work
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Totipotent non-committed inner cell mass (ICM) cells from human blastocysts, if demonstrated to be capable of proliferating in vitro without differentiation, will have several beneficial uses, not only in the treatment of neurodegenerative and genetic disorders, but also as a model in studying the events involved in embryogenesis and genomic manipulation. Nine patients admitted to an in-vitro fertilization programme donated 21 spare embryos for this study. All 21 embryos were grown from the 2-pronuclear until blastocyst stages on a human tubal epithelial monolayer in commercial Earles medium (Medicult, Denmark) supplemented with 10% human serum. The medium was changed after blastocyst formation to Changs medium supplemented with 1000 units/ml of human leukaemia inhibitory factor (HLIF) and the embryos left undisturbed for 72 h to allow the hatched ICM and trophoblast to attach to the feeder monolayer. Nineteen of the 21 embryos from nine patients produced healthy ICM lumps which could be ...
Selective labelling of polar trophectoderm cells in early mouse blastocysts has allowed the fate of polar cells to be followed during in vitro and in vivo blastocyst development. Results show that there is cell movement from polar to mural regions as blastocysts grow. This indicates that trophectode …
The mural trophectoderm is the region of the trophoblast that corresponds to the area of the embryonic sphere opposite and farthest from the inner cell mass. In mouse embryos, this is the site of implantation, whereas in the human embryo implantation is initiated in the opposing polar trophectoderm, nearest to the inner cell mass. The mural trophectoderm gives rise to the chorion leave portion of the placenta ...
The cell lineage of the mouse was studied from the 2-cell stage to the blastocyst. Lineage to the 8-cell stage was followed under the microscope. Each cell from the 2-cell stage divided to form two daughter cells which remained attached. Subsequently, these two daughters each produced two descendants; one of these descendants regularly lay deep in the structure of the embryo while the other was peripheral. Lineage to the blastocyst was followed by injecting oil drops into cells at the 8-cell stage, and then following the segregation of these drops into the inner cell mass and trophectoderm. Between the 8-cell stage and the blastocyst, the deep cells contributed more frequently to the inner cell mass than did the peripheral cells.. ...
Definition of Cell Mass in the Legal Dictionary - by Free online English dictionary and encyclopedia. What is Cell Mass? Meaning of Cell Mass as a legal term. What does Cell Mass mean in law?
The effect of glucose and insulin on the in vitro development of the rat preimplantation embryo was studied by incubating rat blastocysts recovered on days 5 or 6 of pregnancy in the absence or presence of increasing levels of glucose and/or insulin for 24 or 48 h. A differential cell-staining method allowed the separate counting of inner cell mass (ICM) and trophectoderm (TE) cells at the end of the incubation period. In a high-glucose medium (17 mM), ICM and, to a lesser extent, TE developments were significantly and irreversibly inhibited. Low insulin concentrations (3 pM) stimulated ICM and TE development in the presence of 1.1 or 6 mM glucose. Higher insulin levels (30-600 pM) in a 6-mM glucose medium, resulted in a dose-dependent inhibition of ICM and, to a lesser extent, TE development after both 24 and 48 h. This insulin-induced inhibition was reversible if insulin was removed from the medium after 24 h. In the absence of glucose in the medium, insulin was neither stimulatory nor ...
Trophectoderm TE Definition - The trophectoderm is a layer of cells on the outer edge of a blastocyst. The trophectoderm begins to form on the 5th day...
Sepulveda-Rincon, Lessly P. and Dube, Delphine and Adenot, Pierre and Laffont, Ludivine and Ruffini, Sylvie and Gall, Laurence and Campbell, Bruce K. and Duranthon, Veronique and Beaujean, Nathalie and Maalouf, Walid E. (2016) Random allocation of blastomere descendants to the trophectoderm and ICM of the bovine blastocyst. Biology of Reproduction, 95 (6). pp. 1-10. ISSN 1529-7268 ...
(a) The body mass index (BMI) and β‐cell mass were linearly correlated in control group 1 (r2 = 0.64, P = 0.003) and diabetic patients (r2 = 0
A preparation and a method of making composite blastocysts (CBs) from aggregates of dissociated cells of non-viable pre-embryos are disclosed. The CB is characterized morphologically by having two distinct tissue types, the inner cell mass (ICM) and the trophectoderm (TE), and a blastocoelic cavity (BC). The ICM is differentially stainable with bisbenzimide and the TE is differentially stainable with propidium iodide. The ICM is pluripotent in that it contains embryonic stem (ES) cells. The TE cells are pluripotent in that they can give rise to all cell types normally derived from TE cells. The primate TE is characterized by the production of chorionic gonadotrophin. The method of making CBs is an aggregation process (AP) comprising inter alia the following steps: 1) dissociation of discarded pre-embryos; 2) isolation of single nucleated cells from dissociated discarded pre-embryos; 3) microsurgical encapsulation of several cells within a host zona pellucida or artificial aggregation with or without a
The term Endoderm as it applies to the area of stem cell research can be defined as The innermost layer of the cells derived from the inner cell mass of the blastocyst; it gives rise to lungs, other respiratory structures, and digestive organs, or generally the gut.. ...
Trophectoderm Biopsy Definition - A trophectoderm biopsy is a test that is performed to examine the chromosomes of a developing blastocyst. It...
Molecules drawn in ICM can be cut and pasted into ISIS-Draw and molecules from ISIS-Draw can be cut and pasted into ICM. To perform thes functions the correct settings need to be turned on in ISIS-Draw so that the compound drawing is saved in the clipboard. IN ISIS-DRAW - Go to Options/Settings/General/Copy Mol Rxn file to the clipboard. Compounds drawn in ISIS Draw can be cut and pasted into the ICM Molecular Editor and into ICM tables. Compounds can be copied in ICM by: ...
Molecules drawn in ICM can be cut and pasted into ISIS-Draw and molecules from ISIS-Draw can be cut and pasted into ICM. To perform thes functions the correct settings need to be turned on in ISIS-Draw so that the compound drawing is saved in the clipboard. IN ISIS-DRAW - Go to Options/Settings/General/Copy Mol Rxn file to the clipboard. Compounds drawn in ISIS Draw can be cut and pasted into the ICM Molecular Editor and into ICM tables. Compounds can be copied in ICM by: ...
Out of all the creatine products I have ever tried or used, BSNs Cell Mass has worked best for me, as for muscle and strength gains. Before we delve into why I liked the product, lets first answer this question: what is Creatine and what does it do? Creatine is a nutrient naturally found in all…
With the exception of birds, most non-mammalian vertebrates depend on the production of a relatively large number of eggs with only a small proportion surviving. Mammals have evolved a system wherein relatively few eggs are produced, but the survival rate is relatively high. This is accomplished by a combination (in all except the prototheres) of prenatal care and, in most, post-natal care.. In the generalized early development in eutherian mammals, the early cleavages produce the blastocyst, a hollow ball of cells. A cluster of cells intrude into the cavity of the blastocyst: the inner cell mass. This eventually will produce the embryo and some extraembryonic membranes. The outer layer of the blastocyst is the trophoblast. The trophoblast eventually develops into the chorion of the placenta and associated structures.. The placenta forms the connection between mother and child, allowing for nutrition, gases, and excretionary materials to move by diffusion between embryonic and maternal ...
Assisted hatching of day 3 embryos is done to help implantation. The other shell of the embryo is opened with a laser to allow the inner cell mass to implant in the uterus
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Early development and implantation of the embryo.. A. The zygote stage begins upon fertilization of the secondary oocyte by the sperm. The zygote contains both pro-nuclei and is contained within the zona pellucida, until the blastocyst stage. B. The morula stage. Following compaction and symmetrical cleavage divisions of the blastomeres (the cells of the early embryo), the embryo contains 8 (early morula) to 32 cells (morula). The inner cells will give rise to the inner cell mass, whereas the outer cells will give rise to the trophoblast, which forms a cavity called the blastocoele cavity. C. The blastocyst stage. The developing embryo is defined as a blastocyst from the appearance of the blastocoele cavity, and now contains two cell populations- the surrounding outer trophoblast cells, and the inner cell mass cells, located at one side of the inner cavity. The portion of the trophoblast nearest to the inner cell mass is called the polar trophoblast (embryonic pole) and the portion of the ...
Looking for online definition of trophectoderm in the Medical Dictionary? trophectoderm explanation free. What is trophectoderm? Meaning of trophectoderm medical term. What does trophectoderm mean?
Even before implantation, three cell lineages are apparent in the blastocyst of mouse and human. Outermost is the trophectoderm that will contribute trophoblast to the placenta. The inner cell mass has already differentiated into the epiblast and the primitive endoderm or hypoblast. For mouse, this much has been clear since the pioneering studies of Emil Selenka (here). ...
By 4 days after conception (well before implantation) the human zygote (what those who want abortion-on-demand misidentify as a "fertilized egg") gives rise to the blastocyst stage. The blastocyst has 2 cell layers - the trophectoderm, which becomes the placenta, and the inner cell mass, which forms the fetus. The placenta is only partially the mothers, and is mostly developed by the baby. In fact, the tissues of the baby may touch those of the mother, but they do not merge. The blood supply of the mother is never in contact with that of the baby. I write about the placenta, because that is the only point where there could be contention on where the babys body ends and the womans body begins ...
In the article by Saisho et al. (1), the effect of obesity on β-cell mass and turnover was addressed by indirectly estimating β-cell mass from human pancreatic specimens after death related to disease or trauma. Although these results are consistent with the direct measurement of islet volume after isolation from abruptly deceased healthy organ donors, the correlation between β-cell mass and BMI remains weak (r ≤ 0.5) (2,3). The authors admit that cross-sectional studies provide limited information on the mechanisms that potentially link obesity with this increased (50%) β-cell mass. Rather unexpectedly, for example, no measurable difference in β-cell proliferation could be detected by Saisho et al. in pancreatic sections from obese individuals. Despite continued progress in clinical islet noninvasive imaging (4), the current lack of an efficient, quantitative method to assess β-cell mass in the native pancreas prohibits the direct and longitudinal study of islet adaptation to ...
Centro de Investigaciones Científicas Isla de la Cartuja. C/Américo Vespucio, 49 - 41092 Sevilla (España). Tel.: [+34] 954489527 , Fax: [+34] 954460165 , [email protected] ...
Imaging Cytometry and Microfluidics (ICM) core I (Ozinsky) Overview. The core was established by the Center to provide the resources for automated high-throughp...
Oct4 is required for lineage priming in the developing inner cell mass of the mouse blastocyst[4] "The transcription factor Oct4 is required in vitro for establishment and maintenance of embryonic stem cells and for reprogramming somatic cells to pluripotency. In vivo, it prevents the ectopic differentiation of early embryos into trophoblast. Here, we further explore the role of Oct4 in blastocyst formation and specification of epiblast versus primitive endoderm lineages using conditional genetic deletion. Experiments involving mouse embryos deficient for both maternal and zygotic Oct4 suggest that it is dispensable for zygote formation, early cleavage and activation of Nanog expression. Nanog protein is significantly elevated in the presumptive inner cell mass of Oct4 null embryos, suggesting an unexpected role for Oct4 in attenuating the level of Nanog, which might be significant for priming differentiation during epiblast maturation. Induced deletion of Oct4 during the morula to blastocyst ...
Some component in the rat hybridoma supernatant influenced gamma retrovirus infection, either with the cellular Pit receptor or with the envelope proteins, when added to human TE cells and gamma retrovirus ...
Stem cells are biological cells found in all multicellular organisms, that can divide through mitosis and differentiate into diverse specialized cell types and can self renew to produce more stem cells. In mammals, there are two broad types of stem cells: embryonic stem cells that are isolated from the inner cell mass of blastocysts, and…
So make sure you provide enough vitamin E to fertility problems in dogs. Chances are that stem cells are extracted from the while the frost is still in the ground. If one does not like consuming this tissue of the inner cell mass of a blastocyst, an embryo that is 4-5 days old. CSP cultivars are unusually studies are still going on. Vitamin E deficiency in dogs has also been linked juice helps the body excrete excess sodium, thereby reducing water retention. Rub the contents of vitamin E, a couple of times every day and within 2 diabetic retinopathy, Bests disease, or some eye injury or trauma.. ... [Read more…]. ...
This Valentines weekend some American zoos are offering an adults-only opportunity to discover animals amorous antics, and perhaps pick up a few tips.
Embryonic stem cells (ESCs) are the cells of the inner cell mass of a blastocyst, an early-stage embryo.[12] Human embryos reach the blastocyst stage 4-5 days post fertilization, at which time they consist of 50-150 cells. ESCs are pluripotent and give rise during development to all derivatives of the three primary germ layers: ectoderm, endoderm and mesoderm. In other words, they can develop into each of the more than 200 cell types of the adult body when given sufficient and necessary stimulation for a specific cell type. They do not contribute to the extra-embryonic membranes or the placenta. During embryonic development these inner cell mass cells continuously divide and become more specialized. For example, a portion of the ectoderm in the dorsal part of the embryo specializes as neurectoderm, which will become the future central nervous system.[13] Later in development, neurulation causes the neurectoderm to form the neural tube. At the neural tube stage, the anterior portion undergoes ...
Pluripotent embryonic stem (ES) cells were developed as a tool for introducing specific, site-directed alterations into the mammalian genome to create mouse models in which gene function and regulation can be studied. ES cell lines are derived in vitro from the outgrowth of the inner cell mass (ICM) of a blastocyst, the portion of the blastocyst that gives rise to the embryo. Undifferentiated ES cells are then manipulated in culture and, when injected into a blastocyst, have the ability to incorporate back into the ICM and contribute to the genetic makeup of the developing embryo. The resulting pups are considered chimeric in their genetic makeup as they consist of tissues deriving from both the ES cells and cells within the ICM of the blastocyst. The desired outcome is to create chimeric mice that inherit germ cells derived from the injected ES cells. In this way, mutations can be introduced into the ES cells in vitro, then incorporated in vivo into the germline of a mouse and transmitted from ...
Embryonic stem cells are derived from the inner cell mass (ICM) of the blastocyst. They can proliferate indefinitely in vitro (self renewal) and…
Embryonic stem cells are derived from the inner cell mass (ICM) of the blastocyst. They can proliferate indefinitely in vitro (self renewal) and…
Embryonic stem cells are derived from the inner cell mass (ICM) of the blastocyst. They can proliferate indefinitely in vitro (self renewal) and…
after the human zygote has underone three complete cell cycles, it consists of 8 identicle cells.. each of these cells are said to be Totipotent stem cells as they can develop into a complete human being.. by 5 days after conception a hollow ball of cells called the blastocyst has formed.. the outer blastocyst layer goes on to form the placenta and the inner cell mass of 50 or so cells goes on to form the tissues of the developing embryo.. these cells are known as the Pluripotent embryonic stem cells.. each of these cells can potentially give rise to most types of cells but not all. ...
Wiki) "After fertilization, the mammalian cells, called blastomeres, undergo rotational cleavage until they are at the 16-cell stage called the morula. The morula has a small group of internal cells surrounded by a larger group of external cells. These internal cells are called the inner cell mass (ICM) and will go on to become the actual embryo. The external, surrounding cells develop into the trophoblast cells. However, at this stage there is no cavity within the morula; the embryo is still a ball of dividing cells. In a process called cavitation, the trophoblast cells secrete fluid into the morula to create a blastocoel, the fluid-filled cavity. The membranes of the trophoblast cells contain sodium (Na+) pumps, Na+/K+- ATPase and Na+/H+ exchangers, that pump sodium into the centrally forming cavity. The accumulation of sodium pulls in water osmotically, creating and enlarging the blastocoel within the mammalian embryo.[7][8][16] The oviduct cells stimulate these trophoblast sodium pumps as ...
Explaination: Explanation: Placenta and chorion compose the fetal-maternal junction. The former is a trophoblast derivative and the latter is a derivative of both trophoblast and extraembryonic mesoderm. The other extraembryonic tissues such as the amnion, the yolk sac and the allantois are derived from the inner cell mass. Among them, the yolk sac and the allantois are endodermal derivatives whereas the amnion is an ectodermal derivative ...
Abstract Oct-4 is a maternally expressed octamer-binding protein encoded by the murine Oct-4 gene. It is present in unfertilized oocytes, but also in the inner cell mass and in pri..
Interested in mass cytometry? Single cell mass cytometry for identification of rare cancer cell subtypes: an interview with Wendy Fantl & Veronica Gonzalez
The pre-implantation genetic analysis is a set of examinations which can help as reveal genetic abnormalities of embryo before it is transferred to the mother.
Early blastocyst stage 4 Random X-inactivation in the embryonic lineage (inner cell mass) Late blastocyst stage Late blastocyst ... In the early blastocyst, this initial, imprinted X-inactivation is reversed in the cells of the inner cell mass (which give ... XaP XaM → undergoing random X-inactivation in the embryonic lineage (inner cell mass) in the blastocyst stage, leading to: ... 2-4 cell stage[11] 2-8 cell stage[11] 2 Imprinted (paternal) X-inactivation 4-8 cell stage[10][12] Unclear if it takes place in ...
Enders A. C., Lantz K. C., Schlafke S. (1990). Differentiation of the inner cell mass of the baboon blastocyst. Anat. Rec. 226 ... The hypoblast is a tissue type that forms from the inner cell mass. It lies beneath the epiblast and consists of small cuboidal ... Cell 6, 167-181. Duval M. (1891). The rodent placenta. Third part. The placenta of the mouse and of the rat. J. Anat. Physiol. ... Cell 3, 735-744. Perea-Gomez A., Vella F. D., Shawlot W., Oulad-Abdelghani M., Chazaud C., Meno C., Pfister V., Chen L., ...
Mammals at this stage form a structure called the blastocyst,[1] characterized by an inner cell mass that is distinct from the ... expansion of one cell sheet over other cells[2] Ingression - migration of individual cells into the embryo (cells move with ... On the deep surface of the inner cell mass, a layer of flattened cells, called the endoderm, is differentiated and quickly ... The fertilized egg cell is known as the zygote. Cell division with no significant growth, producing a cluster of cells that is ...
However, the egg can split into two, but still have one blastocyst. This will lead to one inner cell mass and one blastocyst. ... The egg will then completely split into two, normally at the two-cell stage. If the egg splits in the early blastocyst stage, ... two inner cell masses will be present, eventually leading to the twins sharing the same chorion and placenta, but with separate ...
"Embryonic stem cell lines derived from human blastocysts." Science. 1998; 282:1145-1147. Thomson, JA. "Primate Embryonic Stem ... Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, Jones JM. " ... "Primate Embryonic Stem Cells." U.S. Patent 6,200,806. 13 March 2001. Thomson, JA. "Primate Embryonic Stem Cells." U.S. Patent ... Loring, J. "A Patent Challenge for Human Embryonic Stem Cell Research." Nature Reports Stem Cells. 2007. Takahashi K, Yamanaka ...
A morula is distinct from a blastocyst in that a morula (3-4 days post fertilization) is a 16-cell mass in a spherical shape ... This results in a hollow ball of cells known as the blastocyst. The blastocyst's outer cells will become the first embryonic ... whereas a blastocyst (4-5 days post fertilization) has a cavity inside the zona pellucida along with an inner cell mass. A ... epithelium (the trophectoderm). Some cells, however, will remain trapped in the interior and will become the inner cell mass ( ...
The inner cell mass of the blastocyst divides rapidly, forming two layers. The top layer becomes the embryo, and cells from ... The presence of the blastocyst means that two types of cells are forming, an inner-cell mass growing on the interior of the ... The cells surrounding the blastocyst now destroy cells in the uterine lining, forming small pools of blood, which in turn ... The cells on the exterior of the blastocyst begin excreting an enzyme which erodes epithelial uterine lining and creates a site ...
... the apposition on the blastocyst is not dependent on if it is on the same side of the blastocyst as the inner cell mass. Rather ... The decidual cells originate from the stromal cells that are always present in the endometrium. However, the decidual cells ... the inner cell mass rotates inside the trophoblast to align to the apposition. In short, the entire surface of the blastocyst ... refers to the transformation that occurs as the boundaries between these cells disappear to form a single mass of many cell ...
The fertilized egg or the zygote becomes a blastocyst where the outer layer and the inner cell mass differentiate to form ... It is also in this stage where the blastocyst develops into an embryo, where all major features of human are present and ... Maternal body weight is determined by the Body Mass Index (BMI) which is defined as the weight in kilograms divided by the ... The first column categorizes the type of body weight based on the Body Mass Index. The second column summarizes the total ...
"Efficient isolation inner cell mass from blastocysts by improved microsurgical technique". Cell Research (2008) 18:s39. doi: ... This technique is used to isolate the inner cell mass of the blastocyst. The trophoectoderm's cell junctions and tight ... "Laser-assisted blastocyst dissection and subsequent cultivation of embryonic stem cells in a serum/cell free culture system: ... Immunosurgery is a method of selectively removing the external cell layer (trophoblast) of a blastocyst through a cytotoxicity ...
It is responsible for moving cells toward the inner cell mass in developing blastocysts. CARM1 plays an important role in ... CARM1-mediated MDH1 methylation reduces cellular NADPH level and sensitizes cells to oxidative stress, thereby suppressing cell ... Cancer Cell. 30 (1): 179-180. doi:10.1016/j.ccell.2016.06.013. PMID 27479032. Wang YP, Zhou W, Wang J, Huang X, Zuo Y, Wang TS ... Ma H, Hong H, Koh SS, Huang SM, Schurter BT, Aswad DW, Stallcup MR (1999). "Regulation of transcription by a protein ...
This then changes to a blastocyst, consisting of an outer layer called a trophoblast, and an inner cell mass called the ... Filled with uterine fluid, the blastocyst breaks out of the zona pellucida and undergoes implantation. The inner cell mass ... Through cell signaling cascades and interactions with the ectodermal and endodermal cells, the mesodermal cells begin the ... In humans, after about three days, the zygote forms a solid mass of cells by mitotic division, called a morula. ...
Cell division continues at a rapid rate and the cells then develop into what is known as a blastocyst. The blastocyst arrives ... The development of the mass of cells that will become the infant is called embryogenesis during the first approximately ten ... Cell division begins approximately 24 to 36 hours after the male and female cells unite. ... The sperm and the egg cell, which has been released from one of the female's two ovaries, unite in one of the two fallopian ...
Thomson; Itskovitz-Eldor, J; Shapiro, SS; Waknitz, MA; Swiergiel, JJ; Marshall, VS; Jones, JM (1998). "Blastocysts Embryonic ... "NIH Stem Cell Basics. What are embryonic stem cells?". 2016년 8월 31일에 원본 문서에서 보존된 문서. 2016년 8월 19일에 확인함.. ... "DNA repair by nonhomologous end joining and homologous recombination during cell cycle in human cells". 》Cell Cycle (Georgetown ... "Cell Stem Cell》 2 (1): 10-12. ISSN 1875-9777. PMID 18371415. doi:10.1016/j.stem.2007.12.001.. ...
... the onset of mtDNA replication is specific to the cells of the trophectoderm. In contrast, the cells of the inner cell mass ... exploiting cell-to-cell variability to ameliorate the inheritance of damaging mutations. At the blastocyst stage, ... The bottleneck exploits stochastic processes in the cell to increase in the cell-to-cell variability in mutant load as an ... Cell-level selection may then act to remove those cells with more mutant mtDNA, leading to a stabilisation or reduction in ...
Cell division continues at a rapid rate and the cells then develop into what is known as a blastocyst. The blastocyst arrives ... The development of the mass of cells that will become the infant is called embryogenesis during the first approximately ten ... Cell division begins approximately 24 to 36 hours after the male and female cells unite. ... During this time, cells begin to differentiate into the various body systems. The basic outlines of the organ, body, and ...
They developed the idea of using blastocysts for the isolation of embryonic stem cells. After Kaufman left to take up a ... Massachusetts, for one month of practical work to learn the most recent laboratory techniques. In the 1990s, he was a fellow at ... They explored the method of using blastocysts for the isolation of embryonic stem cells. After Kaufman left, Evans continued ... These early embryonic cells have the potential to differentiate into any of the cells of the adult organism. They modified ...
Hewitson, L.C., Leese, H.J. (1993) Energy metabolism of the trophectoderm and inner cell mass of the mouse blastocyst. J Exp ... Schatten G, Hewitson L, Simerly C, Sutovsky P and Huszar G. (1998) Cell and Molecular Biological Challenges of ICSI: A.R.T. ... Hewitson L, Takahashi D, Dominko T, Simerly C, and Schatten G. (1998) Fertilization and embryo development to blastocysts after ... 1999) Births of ICSI monkeys: unique checkpoints during the first cell cycle of fertilization. Nature Medicine 5:431-433. ...
In mammals the blastula is referred to as a blastocyst. The blastocyst contains an embryoblast (or inner cell mass) that will ... Blastula-stage cells can behave as pluripotent stem cells in many species. Pluripotent stem cells are the starting point to ... The vegetal mass is composed of the blastocoel floor and primarily develops into endodermal tissue. In the mammalian blastocyst ... The addition of the two growth phases into the cell cycle allows for the cells to increase in size, as up to this point the ...
The inner cells are pushed to one side of the cavity (because the embryo isn't getting any bigger) to form the inner cell mass ... At this stage, the embryo is called a blastocyst. Embryogenesis Blastocyst Forgács, G.; Newman, Stuart A. (2005). "Cleavage and ... Polar cells on the outside and apolar cells on the inside. The outer cells, called the trophoblast cells, pump sodium in from ... In unequal cleavage, the first two cell divisions are unequal producing four cells in which one cell is bigger than the other ...
2003). "Human blastocysts and endometrial epithelial cells express activated leukocyte cell adhesion molecule (ALCAM/CD166)". J ... Bajorath J, Bowen MA, Aruffo A (1996). "Molecular model of the N-terminal receptor-binding domain of the human CD6 ligand ALCAM ... a new cell adhesion molecule in metastasizing human melanoma cell lines, is identical to ALCAM (activated leukocyte cell ... a new cell adhesion molecule in metastasizing human melanoma cell lines, is identical to ALCAM (activated leukocyte cell ...
... the inner cell mass (embryoblast) also develops. The inner cell mass is the source of embryonic stem cells, which are ... The epiblast is adjacent to the trophoblast and made of columnar cells; the hypoblast is closest to the blastocyst cavity and ... Cells differentiate into an outer layer of cells (collectively called the trophoblast) and an inner cell mass. With further ... epithelial cells become mesenchymal stem cells, multipotent stromal cells that can differentiate into various cell types. The ...
The pluripotent cell types that comprise embryoid bodies include embryonic stem cells (ESCs) derived from the blastocyst stage ... whereby cell specification of the inner cell mass results in the formation of the visceral endoderm and epiblast. As the ... "Mass Transfer Limitations in Embryoid Bodies during Human Embryonic Stem Cell Differentiation". Cells Tissues Organs. 196 (1): ... including somatic cell nuclear transfer or the reprogramming of somatic cells to yield induced pluripotent stem cells (iPS). ...
Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, Jones JM (1998). "Embryonic stem cell lines ... derived from human blastocysts". Science (New York) 282 (5391): 1145-7. PMID 9804556. ... Lindvall O (2003). "Stem cells for cell therapy in Parkinson's disease". Pharmacol Res 47 (4): 279-87. PMID 12644384. ... "Researchers find new method for turning adult cells into stem cells". Amherst Daily News. Canadian Press. 2009-01-03. Vaadatud ...
... ectoplacental cone and secondary giant cells in mouse blastocysts reconstituted from isolated trophoblast and inner cell mass ... Gardner, R.L. and Lyon, M.F. (1971). X-chromosome inactivation studied by injection of a single cell into the mouse blastocyst ... Gardner, R. L. (9 November 1968). "Mouse Chimaeras obtained by the Injection of Cells into the Blastocyst". Nature. 220 (5167 ... Gardner pioneered the transplantation of cells and tissues between blastocyst stage mouse embryos and their reconstruction from ...
Harvard University Press, Cambridge, MA. Further reading[edit]. *. "Every Cell in Your Body Has the Same DNA. Except It Doesn't ... of blastocyst-stage embryos derived from in vitro fertilization.[3] ... The 46/47 annotation indicates that the XY cells have the normal number of 46 total chromosomes, and the XXY cells have a total ... After further rounds of replication, this cell would result in a patch, or "clone" of cells mutant for the allele being studied ...
... and cells of the trophoblast and inner cell mass of the mouse blastocyst. This activity is specifically removed by absorption ... and cells of the trophoblast and inner cell mass of the mouse blastocyst. This activity is specifically removed by absorption ... and cells of the trophoblast and inner cell mass of the mouse blastocyst. This activity is specifically removed by absorption ... and cells of the trophoblast and inner cell mass of the mouse blastocyst. This activity is specifically removed by absorption ...
... the early mouse embryo consists of three distinct cell lineages: the epiblast (EPI), primitive endoderm (PrE), and ... Inner Cell Mass Asymmetric Division Cell Fate Decision Lineage Bias Inner Cell Mass Cell These keywords were added by machine ... 2012) Cell Lineage Allocation Within the Inner Cell Mass of the Mouse Blastocyst. In: Kubiak J. (eds) Mouse Development. ... Cell Lineage Allocation Within the Inner Cell Mass of the Mouse Blastocyst. ...
Completely ES cell-derived mice produced by tetraploid complementation using inner cell mass (ICM) deficient blastocysts.. [ ... according to the presence or absence of an inner cell mass (ICM). We designate these as type a (presence of ICM at blastocyst ... Our results demonstrate that completely ES cell-derived mice can be produced using ICM-deficient 4n blastocysts, and provide ... whereas when type b blastocysts were used as hosts, the resulting mice are all completely ES cell-derived, with the newborn ...
Publication: Global gene expression of the inner cell mass and trophectoderm of the bovine blastocyst. Published: December 12th ... Ozawa et al.(2012) Global gene expression of the inner cell mass and trophectoderm of the bovine blastocyst. BMC Developmental ... global gene expression patterns in the inner cell mass (ICM) and trophectoderm (TE) isolated from bovine blastocysts. ... blastomeres of the morula-stage embryo into the ICM and TE of the blastocyst is accompanied by differences between the two cell ...
Results show that there is cell movement from polar to mural regions as blastocysts grow. This indicates that trophectode … ... Selective labelling of polar trophectoderm cells in early mouse blastocysts has allowed the fate of polar cells to be followed ... during in vitro and in vivo blastocyst development. ... Interaction between inner cell mass and trophectoderm of the ... This indicates that trophectoderm cells directly opposite the inner cell mass are the oldest mural cells. However, after ...
Bongso, A., Fong, C.-Y., Ng, S.-C., Ratnam, S. (1994). Isolation and culture of inner cell mass cells from human blastocysts. ... Totipotent non-committed inner cell mass (ICM) cells from human blastocysts, if demonstrated to be capable of proliferating in ... Two of the lumps differentiated into fibroblasts while the remaining 17 (eight patients) produced cells with typical stem cell- ... All 21 embryos were grown from the 2-pronuclear until blastocyst stages on a human tubal epithelial monolayer in commercial ...
This study was performed to investigate the rate of inner cell mass of blastocyst which obtain from culture of mouse two cell ... Saki Gh, Sobhani A, Akbari M. The Rate Of Inner Cell Mass Of Blastocysts Which Obtain From Mouse Two Cell Embryos Cultured In ... The Rate Of Inner Cell Mass Of Blastocysts Which Obtain From Mouse Two Cell Embryos Cultured In Absence And Presence Of ... This study was performed to investigate the rate of inner cell mass of blastocyst which obtain from culture of mouse two cell ...
The preimplantation mouse blastocyst. A schematic drawing of the differentiated mouse blastocyst. The inner cell mass (ICM) and ... GLUT8 Localization Within the Mouse Blastocyst.. Two-cell and four-cell embryos were obtained from superovulated (B6 × SJL)F1 ( ... On insulin stimulation, however, both mural and polar trophectoderm cells and possibly primitive endoderm cells show cell ... Two-cell embryos were obtained as described above and cultured to a blastocyst stage under one of the following conditions: (i ...
Global gene expression of the inner cell mass and trophectoderm of the bovine blastocyst. . Biblioteca virtual para leer y ... Global gene expression of the inner cell mass and trophectoderm of the bovine blastocyst - Descarga este documento en PDF. ... Global gene expression of the inner cell mass and trophectoderm of the bovine blastocyst. ... ICMInner cell mass. SSESum of squares error. TFBSTranscription factor binding sites. TETrophectoderm.. Electronic supplementary ...
Howard Hughes Medical Institute, Cambridge, Mass, USA.. Comment in. *New human embryonic stem-cell lines--more is better. [N ... Derivation of embryonic stem-cell lines from human blastocysts.. Cowan CA1, Klimanskaya I, McMahon J, Atienza J, Witmyer J, ... Stem Cells - MedlinePlus Health Information. Research Materials. *Cell lines described in this publication - Cellosaurus - a ... Two fillips for human embryonic stem cells. [N Engl J Med. 2004] ...
... future inner cell mass, ICM. ) are in white. (f) Formation of the blastocyst. Note that the inner cell mass is completely ... g) By 4.5 dpc, the blastocyst has hatched out of its zona pellucida, the inner cell mass has formed primitive endoderm (not ... Rossant J (1975) Investigation of the determinative state of the mouse inner cell mass. I. Aggregation of isolated inner cell ... Cell 95: 379-391. Rashbass P, Cooke LA, Herrmann BG and Beddington RSP (1991) A cell autonomous function of Brachyury in T/T ...
Human stem cells can come from an embryo or an adult human. They have many possible uses in science and medicine, yet ... Stem cells are basic cells that can become almost any type of cell in the body. ... Soon, and before the embryo implants in the uterus, this mass of around 150-200 cells is the blastocyst. The blastocyst ... an inner cell mass that will develop into the human body. The inner cell mass is where embryonic stem cells are found. ...
... but did enhanced proliferation of the inner cell mass in blastocysts (P , 0.05)-which differs from the phenotype reported for ... but did enhanced proliferation of the inner cell mass in blastocysts (P , 0.05)-which differs from the phenotype reported for ... but did enhanced proliferation of the inner cell mass in blastocysts (P , 0.05)-which differs from the phenotype reported for ... but did enhanced proliferation of the inner cell mass in blastocysts (P , 0.05)-which differs from the phenotype reported for ...
José Celso Rocha and others published Using Artificial Intelligence to Improve the Evaluation of Human Blastocyst Morphology ... clear inner cell mass, cohesive trophectoderm; C: small inner cell mass ± irregular trophectoderm ± excluded/degenerate cells) ... inner cell mass (ICM) grade and trophectoderm grade-to predict outcome of a cycle with single-blastocyst transfer. The study ... The first letter is referred to the quality of the inner cell mass and (second letter) of the trophectoderm. (a) 3AA; (b) 3AB ...
... of early mouse and human embryos has created significant interest in this source of ES cells. However, sister BTMs of an early ... Blastocyst / cytology*. Blastocyst Inner Cell Mass / cytology. Blastomeres / cytology*. Cell Count. Cell Culture Techniques. ... Cell Differentiation. Cells, Cultured. Cleavage Stage, Ovum. Embryonic Stem Cells / cytology*. Female. Homeodomain Proteins / ... from four-cell stage. Four ES cell lines (5.6%; 4/72) were established ater culture of single BTMs from two-cell embryos, and ...
Examination of plastic-embedded rhesus monkey and baboon blastocysts through the implantation period has provided information ... The definitive yolk sac is formed by an aggregation of endodermal cells adjacent to the inner cell mass. The trophoblast does ... An amnionic cavity is formed within the inner cell mass, and is preceded by establishment of cell polarity. ... including differentiation of endoderm and its extension beyond the inner cell mass prior to implantation. However, there ...
73524099 - stem cells. These inner cell mass from a blastocyst. These stem.. Vector ... 50243546 - Stem cell. The development of red blood cells, leukocytes, macrophages,.. Vector ... 41096835 - Bone marrow, stem cells of human bone marrow stem cells. These.. ... 41095429 - Bone marrow, stem cells of human bone marrow stem cells. These.. ...
Day 5 blastocyst IVF embryos of differing quality are shown. We specialize in blastocyst transfer. Chicago, IL. ... Early blastocyst grading photos:. Early blastocyst Cavity very small = Cant yet grade. Inner cell mass = Cant yet grade. ... Inner cell mass = Cant yet grade. Trophectoderm = Cant yet grade 1AB Cavity , 1/2 embryos volume = 1. Inner cell mass = A. ... The clump of cells in the 10 to 12 oclock area is the inner cell mass (ICM) which become the fetus. The trophectoderm cells ( ...
Tbx3 is expressed earliest, in the inner cell mass of the blastocyst. Four of the genes are expressed in different components ... across a wide range of embryonic stages from blastocyst through gastrulation and early organogenesis by in situ hybridization ...
Cloning can be used to produce a supply of unspecialized stem cells that can be induced to grow into various types of body ... Stem cells are primitive, unspecialized cells. A 5-day-old human embryo, called a blastocyst, contains an inner cell mass ... In the blastocyst, embryonic cells divide, producing a mass of stem cells. ... The patients skin cell is inserted into the outer membrane of the egg cell and chemically induced to begin developing into a ...
Definition of embryonic stem cell. Provided by Stedmans medical dictionary and Drugs.com. Includes medical terms and ... Definition: totipotent cells composing the inner cell mass of the blastocyst.. Further information. Always consult your ...
Main article: Embryonic stem cell. Embryonic stem cells (ESCs) are the cells of the inner cell mass of a blastocyst, an early- ... Pluripotent, embryonic stem cells originate as inner cell mass (ICM) cells within a blastocyst. These stem cells can become any ... embryonic stem cells, which are isolated from the inner cell mass of blastocysts, and adult stem cells, which are found in ... Stem cell division and differentiation A: stem cell; B: progenitor cell; C: differentiated cell; 1: symmetric stem cell ...
Embryonic stem cells (ESCs) are derived from the inner cell mass of a preimplantation blastocyst, as has been well established ... Embryonic stem cells (ESCs) are derived from the inner cell mass of preimplantation blastocysts. From agricultural and ... inner cell mass. Under specific culture conditions embryonic stem cells (ESCs) can be captured and expanded from the inner cell ... 2014) The ability of inner-cell-mass cells to self-renew as embryonic stem cells is acquired following epiblast specification. ...
The Genetically Engineered Mouse Facility at MD Anderson offers many services including the generation of ES Cells from ... Blastocysts will be isolated and grown to obtain the inner cell mass. The ICM will then be dissociated to obtain ES cells. ES ... Generation of ES Cells from Blastocysts. The GEMF is now able to generate novel ES cell lines from blastocysts. This technique ... Day 12: Blastocyst outgrowth is checked for the presence of a distinct inner cell mass (ICM). If a defined ICM is detected, it ...
Mesoderm-The middle layer of the embryo, which consists of a group of cells derived from the inner cell mass of the blastocyst ... terior (the inner cell mass) (IOM, 2005, p. 115). Cells from the inner cell mass, if grown in culture, can give rise to ... Germ cell (or germline cell)-A cell at any point in the lineage of cells that will give rise to sperm or eggs. The germline is ... Spermatogonial stem cells-The self-replicating precursors of sperm cells.. Stem cell-A nonspecialized cell that has the ...
  • Unipotent cells can produce only one cell type, their own, but have the property of self-renewal, which distinguishes them from non-stem cells (e.g. progenitor cells, which cannot self-renew). (wikipedia.org)
  • Additionally, the mechanism(s) by which engrafted stem/progenitor cells exert their actions are to a large extent unknown. (frontiersin.org)
  • Haematopoietic progenitor cells were first reported to be present in the amniotic fluid in 1993, specifically up to the 12th week of pregnancy. (wikipedia.org)
  • In 1996, a study indicated that non-haematopoietic progenitor cells were also present in the amniotic fluid. (wikipedia.org)
  • Pluripotent stem cells undergo further specialization into multipotent progenitor cells that then give rise to functional cells. (wikipedia.org)
  • Examples of stem and progenitor cells include:[citation needed] Radial glial cells (embryonic neural stem cells) that give rise to excitatory neurons in the fetal brain through the process of neurogenesis. (wikipedia.org)
  • Hematopoietic stem cells (adult stem cells) from the bone marrow that give rise to red blood cells, white blood cells, and platelets Mesenchymal stem cells (adult stem cells) from the bone marrow that give rise to stromal cells, fat cells, and types of bone cells Epithelial stem cells (progenitor cells) that give rise to the various types of skin cells Muscle satellite cells (progenitor cells) that contribute to differentiated muscle tissue. (wikipedia.org)
  • Furthermore, non-CpG methylation has also been observed in hematopoietic progenitor cells, and it occurred mainly in a CpApC sequence context. (wikipedia.org)
  • These temporary cells are called progenitor cells, and are intermediate cell types which disappear during embryogenesis by turning into other progenitor cells, or into mature adult somatic cell types, or which disappear due to programmed cell death (apoptosis). (wikipedia.org)
  • Healthy adult brains contain neural stem cells which divide to maintain general stem-cell numbers, or become progenitor cells. (wikipedia.org)
  • In healthy adult laboratory animals, progenitor cells migrate within the brain and function primarily to maintain neuron populations for olfaction (the sense of smell). (wikipedia.org)
  • Others have shown that insulin and IGF-1 regulate glucose transport in mouse blastocysts via the IGF-1 receptor ( 9 ). (pnas.org)
  • This plasmid library was made from poly(A) RNA from a pool of day-5 mouse blastocysts as described ( 10 ). (pnas.org)
  • Avilion AA, Nicolis SK, Pevny LH, Perez L, Vivian N, Lovell-Badge R (2003) Multipotent cell lineages in early mouse development depend on SOX2 function. (springer.com)
  • Stem cell research" redirects here. (wikipedia.org)
  • For the journal, see Stem Cell Research (journal) . (wikipedia.org)
  • Ryan J. Felling reports grants from NIH/NINDS for stem cell research (K08NS097704). (springer.com)
  • What are the potential applications for stem cell research? (eurostemcell.org)
  • This is an important goal of stem cell research. (eurostemcell.org)
  • You will hear from Museum scientists, medical researchers at the frontiers of the field, and a panel of bioethics experts who will address the ethical implications of stem cell research and therapy. (coursera.org)
  • Stem cell research is actively pursuing strategies to yield new cell sources for use in regenerative medicine. (ahajournals.org)
  • A topic of extended scientific and ethical debate in our society as of late has been the question of Stem Cell research. (brightkite.com)
  • Before I address the debate of whether or not Stem Cell research should be done, I want to first explain to my readers what stem cells are, how they come to be and what we can use them for. (brightkite.com)
  • Now I will get into the possible benefits of Stem Cell research. (brightkite.com)
  • The ICM obtained can then be used for stem cell research and is better to use than adult or fetal stem cells because the ICM has not been affected by external factors, such as manually bisecting the cell. (wikipedia.org)
  • Many believe that the timing of this appeal was a direct response to the increasing licensing costs paid by California taxpayers with the rise of state-funded stem cell research programs, specifically the California Institute for Regenerative Medicine (CIRM). (wikipedia.org)
  • In 2000, the NIH, under the administration of President Bill Clinton, issued "guidelines that allow federal funding of embryonic stem-cell research. (wikipedia.org)
  • No federal law ever did ban stem cell research in the United States, but only placed restrictions on funding and use, under Congress's power to spend. (wikipedia.org)
  • In April 2004, 206 members of Congress, including many moderate Republicans, signed a letter urging President Bush to expand federal funding of embryonic stem cell research beyond what Bush had already supported. (wikipedia.org)
  • On July 29, 2005, Senate Majority Leader William H. Frist (R-TN), announced that he too favored loosening restrictions on federal funding of embryonic stem cell research. (wikipedia.org)
  • On July 18, 2006, the Senate passed three different bills concerning stem cell research. (wikipedia.org)
  • He became a Knight Bachelor in the 2004 New Year Honours in recognition of his work in stem cell research. (wikipedia.org)
  • The study of the blastula and of cell specification has many implications on the field of stem cell research as well as the continued improvement of fertility treatments. (wikipedia.org)
  • Somatic cell nuclear transplantation has become a focus of study in stem cell research. (wikipedia.org)
  • Only a handful of the labs in the world are currently using SCNT techniques in human stem cell research. (wikipedia.org)
  • Dr Chung is a prominent leader in the field of stem cell research with over 20 years of experience. (wikipedia.org)
  • He was a professor of theriogenology and biotechnology at Seoul National University (dismissed on March 20, 2006) who became infamous for fabricating a series of experiments, which appeared in high-profile journals, in the field of stem cell research. (wikipedia.org)
  • On May 12, 2006, Hwang was charged with embezzlement and bioethics law violations after it emerged much of his stem cell research had been faked. (wikipedia.org)
  • The Korea Times reported on June 10, 2007, that Seoul National University fired him, and the South Korean government canceled his financial support and barred him from engaging in stem cell research. (wikipedia.org)
  • In February 2011, Hwang visited Libya as part of a $133 million project in the North African country to build a stem cell research center and transfer relevant technology. (wikipedia.org)
  • Although there are some differences in the abundant ICM cells, there is no distinct endodermal layer. (slideserve.com)
  • Although transcriptional determination has usually been considered as a binary on-off control system, they found that the precise level of Oct-4 governs 3 distinct fates of ES cells. (wikipedia.org)
  • A genetic chimerism or chimera (also spelled chimaera) is a single organism composed of cells with distinct genotypes. (wikipedia.org)
  • An animal chimera is a single organism that is composed of two or more different populations of genetically distinct cells that originated from different zygotes involved in sexual reproduction. (wikipedia.org)
  • because they could only extract a small number of cells, they could not perform both comprehensive gene-expression analysis and DNA fingerprinting on a single blastocyst. (technologyreview.com)
  • The technique consists of taking an enucleated oocyte (egg cell) and implanting a donor nucleus from a somatic (body) cell. (wikipedia.org)
  • The team from the Vincent Center for Reproductive Biology, Massachusetts, Boston showed that oocyte formation takes place in ovaries of reproductive-age women. (wikipedia.org)
  • The process involves sucking out the nucleus of a somatic (body) cell and injecting it into an oocyte that has had its nucleus removed Using an approach based on the protocol outlined by Tachibana et al. (wikipedia.org)
  • Asymmetric division is a process of mitosis in which one oogonium divides unequally to produce one daughter cell that will eventually become an oocyte through the process of oogenesis, and one daughter cell that is an identical oogonium to the parent cell. (wikipedia.org)