Embryo Culture Techniques
Fertilization in Vitro
Blastocyst Inner Cell Mass
Cleavage Stage, Ovum
Embryo Implantation, Delayed
Nuclear Transfer Techniques
Sperm Injections, Intracytoplasmic
Gene Expression Regulation, Developmental
In Vitro Oocyte Maturation Techniques
Single Embryo Transfer
Mice, Inbred Strains
Octamer Transcription Factor-3
Embryonic Stem Cells
Leukemia Inhibitory Factor
Reproductive Techniques, Assisted
An ultrastructural study of implantation in the golden hamster. II. Trophoblastic invasion and removal of the uterine epithelium. (1/4036)Sixty six implantation sites from 18 golden hamsters were examined with light and electron microscopy between 4 and 5 1/2 days of pregnancy (post-ovulation). At 4 days some blastocysts began to invade the uterine epithelium, with trophoblastic processes penetrating and engulfing portions of the uterine epithelium. The majority of epithelial cells appeared normal before invasion, although at two implantation sites three or four adjoining epithelial cells were necrotic before penetration by the trophoblast. In general the epithelial cells were degenerating at the time the trophoblast invaded the epithelium. Inclusions, representing portions of the engulfed epithelium, and varying in size and electron density, were present throughout the invading trophoblast cells at 4 1/2 and 5 days of pregnancy. At 5 1/2 days the uterine epithelium had disappeared and the embryo was now almost completely surrounded by blood lacunae. (+info)
Ontogeny of expression of a receptor for platelet-activating factor in mouse preimplantation embryos and the effects of fertilization and culture in vitro on its expression. (2/4036)Platelet-activating factor (PAF; 1-o-alkyl-2-acetyl-sn-glycero-3-phosphocholine) is a potent ether phospholipid. It is one of the preimplantation embryo's autocrine growth/survival factors. It may act via a G protein-linked receptor on the embryo; however, the evidence for this is conflicting. The recent description of the intracellular form of the PAF:acetlyhydrolase enzyme as having structural homology with G proteins and Ras also suggests this as a potential intracellular receptor/transducer for PAF. This study used reverse transcription-polymerase chain reaction to examine the ontogeny of expression of the genes for these proteins in the oocyte and preimplantation-stage embryo. Transcripts for the G protein-linked PAF receptor were detected in the late 2-cell-stage embryo and in all stages from the 4-cell stage to blastocysts. They were also present in unfertilized oocytes and newly fertilized zygotes but only at relatively low levels. The incidence of expression was generally low and variable in late zygotes and early 2-cell embryos. Expression past the 2-cell stage was alpha-amanitin sensitive. The results indicated that mRNA for this receptor is a maternal transcript that was degraded during the zygote-2-cell stage. New expression of the receptor transcript required activation of the zygotic genome. Fertilization of embryos in vitro caused this transcript not to be expressed in the zygote. Culture of zygotes (irrespective of their method of fertilization) caused expression from the zygotic genome to be retarded by more than 24 h. This retardation did not occur if culture commenced at the 2-cell stage. The transcripts for the subunits of intracellular PAF:acetylhydrolase were not detected in oocytes or at any stage of embryo development examined, despite their being readily detected in control tissue. This study confirms the presence of the G protein-linked PAF receptor in the 2-cell embryo and describes for the first time its normal pattern of expression during early development. The adverse effects of in vitro fertilization (IVF) and embryo culture on the expression of this transcript may be a contributing factor for the poor viability of embryos produced in this manner. The reduced expression of PAF-receptor mRNA following IVF predicts that such embryos may have a deficiency in autocrine stimulation and also suggests that supplementation of growth media with exogenous PAF would be only partially beneficial. The effect of IVF and culture may also explain the conflicting literature. (+info)
X inactive-specific transcript (Xist) expression and X chromosome inactivation in the preattachment bovine embryo. (3/4036)Expression of the X inactive-specific transcript (Xist) is thought to be essential for the initiation of X chromosome inactivation and dosage compensation during female embryo development. In the present study, we analyzed the patterns of Xist transcription and the onset of X chromosome inactivation in bovine preattachment embryos. Reverse transcription-polymerase chain reaction (RT-PCR) revealed the presence of Xist transcripts in all adult female somatic tissues evaluated. In contrast, among the male tissues examined, Xist expression was detected only in testis. No evidence for Xist transcription was observed after a single round of RT-PCR from pools of in vitro-derived embryos at the 2- to 4-cell stage. Xist transcripts were detected as a faint amplicon at the 8-cell stage initially, and consistently thereafter in all stages examined up to and including the expanded blastocyst stage. Xist transcripts, however, were subsequently detected from the 2-cell stage onward after nested RT-PCR. Preferential [3H]thymidine labeling indicative of late replication of one of the X chromosomes was noted in female embryos of different developmental ages as follows: 2 of 7 (28.5%) early blastocysts, 6 of 13 (46.1%) blastocysts, 8 of 11 (72.1%) expanded blastocysts, and 14 of 17 (77.7%) hatched blastocysts. These results suggest that Xist expression precedes the onset of late replication in the bovine embryo, in a pattern compatible with a possible role of bovine Xist in the initiation of X chromosome inactivation. (+info)
Induction of Ig light chain gene rearrangement in heavy chain-deficient B cells by activated Ras. (4/4036)During B cell development, rearrangement and expression of Ig heavy chain (HC) genes promote development and expansion of pre-B cells accompanied by the onset of Ig light chain (LC) variable region gene assembly. To elucidate the signaling pathways that control these events, we have tested the ability of activated Ras expression to promote B cell differentiation to the stage of LC gene rearrangement in the absence of Ig HC gene expression. For this purpose, we introduced an activated Ras expression construct into JH-deleted embryonic stem cells that lack the ability to assemble HC variable region genes and assayed differentiation potential by recombination activating gene (RAG) 2-deficient blastocyst complementation. We found that activated Ras expression induces the progression of B lineage cells beyond the developmental checkpoint ordinarily controlled by mu HC. Such Ras/JH-deleted B cells accumulate in the periphery but continue to express markers associated with precursor B cells including RAG gene products. These peripheral Ras/JH-deleted B cell populations show extensive Ig LC gene rearrangement but maintain an extent of kappa LC gene rearrangement and a preference for kappa over lambda LC gene rearrangement similar to that of wild-type B cells. We discuss these findings in the context of potential mechanisms that may regulate Ig LC gene rearrangement. (+info)
In-vitro fertilization and culture of mouse embryos in vitro significantly retards the onset of insulin-like growth factor-II expression from the zygotic genome. (5/4036)In this study, the effect of in-vitro fertilization (IVF) and culture of mouse embryos in vitro on the normal expression of insulin-like growth factor-II (IFG-II) ligand and receptor was examined. The expression of IGF-II increased in a linear fashion at least up to the 8-cell stage of development. IGF-II expression in embryos collected fresh from the reproductive tract was significantly (P < 0.001) greater than in embryos fertilized in the reproductive tract and cultured in vitro (in-situ fertilized: ISF), and its expression was further reduced (P < 0.001) in IVF embryos at all development stages tested. The expression of IGF-II was significantly (P < 0.001) lower when embryos were cultured individually in 100 microl drops compared with culture in groups of 10 in 10 microl drops of medium. The addition of platelet activating factor to culture medium partially overcame this density-dependent decline of expression. Culture of ISF and IVF zygotes also caused the onset of new IGF-II mRNA transcription from the zygotic genome to be significantly (P < 0.001) retarded, until at least the 8-cell stage of development. This effect was greater (P < 0.05) for IVF than for ISF embryos. Neither IVF nor culture had any obvious effect on IFG-II/mannose-6-phosphate receptor (IGF-IIr) mRNA expression. (+info)
Detection of benzo[a]pyrene diol epoxide-DNA adducts in embryos from smoking couples: evidence for transmission by spermatozoa. (6/4036)Tobacco smoking is deleterious to reproduction. Benzo[a]pyrene (B[a]P) is a potent carcinogen in cigarette smoke. Its reactive metabolite induces DNA-adducts, which can cause mutations. We investigated whether B[a]P diol epoxide (BPDE) DNA adducts are detectable in preimplantation embryos in relation to parental smoking. A total of 17 couples were classified by their smoking habits: (i) both partners smoke; (ii) wife non-smoker, husband smokes; and (iii) both partners were non-smokers. Their 27 embryos were exposed to an anti-BPDE monoclonal antibody that recognizes BPDE-DNA adducts. Immunostaining was assessed in each embryo and an intensity score was calculated for embryos in each smoking group. The proportion of blastomeres which stained was higher for embryos of smokers than for non-smokers (0.723 versus 0.310). The mean intensity score was also higher for embryos of smokers (1.40+/-0.28) than for non-smokers (0.38+/-0.14; P = 0.015), but was similar for both types of smoking couples. The mean intensity score was positively correlated with the number of cigarettes smoked by fathers (P = 0.02). Increased mean immunostaining in embryos from smokers, relative to non-smokers, indicates a relationship with parental smoking. The similar levels of immunostaining in embryos from both types of smoking couples suggest that transmission of modified DNA is mainly through spermatozoa. We confirmed paternal transmission of modified DNA by detection of DNA adducts in spermatozoa of a smoker father and his embryo. (+info)
Co-expression of cytokeratins and vimentin by highly invasive trophoblast in the white-winged vampire bat, Diaemus youngi, and the black mastiff bat, Molossus ater, with observations on intermediate filament proteins in the decidua and intraplacental trophoblast. (7/4036)Histological and immunocytochemical studies of gravid reproductive tracts obtained from the white-winged vampire bat (Diaemus youngi) and the black mastiff bat (Molossus ater) have established that both species develop unusually invasive trophoblast. This is released by the developing discoidal haemochorial placenta, expresses both cytokeratins and vimentin, and invades the myometrium and adjacent tissues (including the ovaries) via interstitial migration within the walls of maternal blood vessels. Hence, this trophoblast is noteworthy for the extent to which it undergoes an epithelial-mesenchymal transformation. In Molossus, it originates from the cytotrophoblastic shell running along the base of the placenta, is mononuclear, and preferentially invades maternal arterial vessels serving the discoidal placenta. This trophoblast may have a role in dilatation of these vessels when the discoidal placenta becomes functional. In Diaemus, the highly invasive trophoblast appears to originate instead from a layer of syncytiotrophoblast on the periphery of the placenta is multinucleated, and vigorously invades both arterial and venous vessels. During late pregnancy, it becomes extensively branched and sends attenuated processes around many of the myometrial smooth muscle fibres. In view of its distribution, this trophoblast could have important influences upon myometrial contractility and the function of blood vessels serving the gravid tract. Other aspects of intermediate filament expression in the uteri and placentae of these bats are also noteworthy. Many of the decidual giant cells in Molossus co-express cytokeratins and vimentin, while the syncytiotrophoblast lining the placental labyrinth in Diaemus late in pregnancy expresses little cytokeratin. (+info)
Trophectoderm differentiation in the bovine embryo: characterization of a polarized epithelium. (8/4036)Blastocytst formation is dependent on the differentiation of a transporting epithelium, the trophectoderm, which is coordinated by the embryonic expression and cell adhesive properties of E-cadherin. The trophectoderm shares differentiative characteristics with all epithelial tissues, including E-cadherin-mediated cell adhesion, tight junction formation, and polarized distribution of intramembrane proteins, including the Na-K ATPase. The present study was conducted to characterize the mRNA expression and distribution of polypeptides encoding E-cadherin, beta-catenin, and the tight junction associated protein, zonula occludens protein 1, in pre-attachment bovine embryos, in vitro. Immunocytochemistry and gene specific reverse transcription--polymerase chain reaction methods were used. Transcripts for E-cadherin and beta-catenin were detected in embryos of all stages throughout pre-attachment development. Immunocytochemistry revealed E-cadherin and beta-catenin polypeptides evenly distributed around the cell margins of one-cell zygotes and cleavage stage embryos. In the morula, detection of these proteins diminished in the free apical surface of outer blastomeres. E-cadherin and beta-catenin became restricted to the basolateral membranes of trophectoderm cells of the blastocyst, while maintaining apolar distributions in the inner cell mass. Zonula occludens protein 1 immunoreactivity was undetectable until the morula stage and first appeared as punctate points between the outer cells. In the blastocyst, zonula occludens protein 1 was localized as a continuous ring at the apical points of trophectoderm cell contact and was undetectable in the inner cell mass. These results illustrate that the gene products encoding E-cadherin, beta-catenin and zonula occludens protein 1 are expressed and maintain cellular distribution patterns consistent with their predicted roles in mediating trophectoderm differentiation in in vitro produced bovine embryos. (+info)
Ethylene glycol is a colorless, sweet-tasting, and highly toxic liquid that is commonly used as a solvent and antifreeze. In the medical field, ethylene glycol poisoning is a serious condition that occurs when someone ingests or inhales large amounts of this substance. Ethylene glycol poisoning can cause a range of symptoms, including nausea, vomiting, abdominal pain, headache, dizziness, confusion, and difficulty breathing. In severe cases, it can lead to kidney failure, seizures, coma, and death. Treatment for ethylene glycol poisoning typically involves the administration of activated charcoal to absorb the poison from the stomach, followed by the use of antidotes such as ethanol or fomepizole to prevent the body from metabolizing the ethylene glycol into toxic compounds. In some cases, dialysis may be necessary to remove the poison from the bloodstream. It is important to note that ethylene glycol is highly toxic and should be handled with care in the medical field. Any spills or leaks should be cleaned up immediately, and proper safety precautions should be taken to prevent accidental exposure.
Octamer Transcription Factor-3 (Oct3/4) is a transcription factor that plays a crucial role in the regulation of gene expression during embryonic development and stem cell maintenance. It is a member of the POU family of transcription factors, which are characterized by a conserved DNA-binding domain called the POU domain. Oct3/4 is expressed in the inner cell mass of the blastocyst, which gives rise to the embryo proper, and in the embryonic stem cells that can differentiate into all cell types of the body. It is also expressed in some adult tissues, such as the brain and testes. In stem cells, Oct3/4 is essential for maintaining their self-renewal capacity and pluripotency, which allows them to differentiate into any cell type in the body. It does this by binding to specific DNA sequences called Octamer boxes, which are located in the promoter regions of genes that are important for stem cell maintenance and differentiation. In addition to its role in stem cells, Oct3/4 has also been implicated in the development of various diseases, including cancer. For example, some cancer cells can reprogram themselves to express Oct3/4, which allows them to evade immune surveillance and continue to grow and divide uncontrollably. Therefore, targeting Oct3/4 may be a promising strategy for the treatment of certain types of cancer.
Embryo loss, also known as miscarriage, is the loss of a developing embryo or fetus before the 20th week of pregnancy. It is a common occurrence, with about 10-20% of known pregnancies resulting in a miscarriage. There are different types of embryo loss, including: 1. Spontaneous abortion: This is the most common type of embryo loss, and it occurs when the embryo or fetus dies naturally and is expelled from the uterus. 2. Therapeutic abortion: This is a medical or surgical procedure performed to end a pregnancy that is deemed to be high-risk or not viable. 3. Missed abortion: This occurs when the embryo or fetus dies, but the body does not expel it naturally. 4. Ectopic pregnancy: This occurs when the embryo implants outside the uterus, usually in the fallopian tube, and can be life-threatening if not treated promptly. The causes of embryo loss can be genetic, hormonal, environmental, or related to the mother's health. Some common risk factors for embryo loss include advanced maternal age, previous miscarriage, certain medical conditions, and exposure to certain substances or environmental factors.
Leukemia Inhibitory Factor (LIF) is a cytokine protein that plays a role in the regulation of hematopoiesis, which is the process of blood cell formation. It is produced by a variety of cells, including macrophages, monocytes, and some types of cancer cells. LIF has several functions in the body, including promoting the survival and proliferation of hematopoietic stem cells, which are the cells that give rise to all types of blood cells. It also plays a role in the differentiation of these cells into specific types of blood cells, such as red blood cells, white blood cells, and platelets. In the medical field, LIF is being studied as a potential therapeutic agent for a variety of conditions, including cancer, autoimmune diseases, and neurological disorders. It has also been shown to have anti-inflammatory effects and may be useful in treating inflammatory diseases such as rheumatoid arthritis.
Progesterone is a hormone that plays a crucial role in the female reproductive system. It is produced by the ovaries and the placenta during pregnancy and is responsible for preparing the uterus for pregnancy and maintaining the pregnancy. Progesterone also helps to regulate the menstrual cycle and can be used as a contraceptive. In addition to its reproductive functions, progesterone has a number of other effects on the body. It can help to reduce inflammation, promote bone density, and regulate mood. Progesterone is also used in medical treatment for a variety of conditions, including menopause, osteoporosis, and certain types of breast cancer. Progesterone is available as a medication in a variety of forms, including oral tablets, injections, and creams. It is important to note that progesterone can have side effects, including nausea, dizziness, and mood changes. It is important to discuss the potential risks and benefits of using progesterone with a healthcare provider before starting treatment.
Polyvinyl Alcohol (PVA) is a synthetic polymer that is commonly used in the medical field as a water-soluble adhesive in medical tapes, dressings, and other medical devices. It is a hydrophilic polymer, meaning it is attracted to water, and is known for its biocompatibility and non-toxicity. PVA is also used as a thickening agent in various medical products, such as eye drops, nasal sprays, and oral solutions. It can help to stabilize the formulation and improve its viscosity, making it easier to apply or use. In addition, PVA has been investigated for its potential use in drug delivery systems, as it can act as a carrier for drugs and help to control their release over time. It has also been used in tissue engineering applications, as it can be used to create hydrogels that mimic the properties of natural tissue. Overall, PVA is a versatile polymer with a wide range of applications in the medical field, thanks to its unique properties and biocompatibility.
In the medical field, RNA, Messenger (mRNA) refers to a type of RNA molecule that carries genetic information from DNA in the nucleus of a cell to the ribosomes, where proteins are synthesized. During the process of transcription, the DNA sequence of a gene is copied into a complementary RNA sequence called messenger RNA (mRNA). This mRNA molecule then leaves the nucleus and travels to the cytoplasm of the cell, where it binds to ribosomes and serves as a template for the synthesis of a specific protein. The sequence of nucleotides in the mRNA molecule determines the sequence of amino acids in the protein that is synthesized. Therefore, changes in the sequence of nucleotides in the mRNA molecule can result in changes in the amino acid sequence of the protein, which can affect the function of the protein and potentially lead to disease. mRNA molecules are often used in medical research and therapy as a way to introduce new genetic information into cells. For example, mRNA vaccines work by introducing a small piece of mRNA that encodes for a specific protein, which triggers an immune response in the body.
Pronase is a proteolytic enzyme that is used in the medical field for various purposes. It is derived from the fungus Streptomyces griseus and is commonly used as a digestive enzyme to break down proteins in the body. In the medical field, Pronase is used to treat a variety of conditions, including: 1. Chronic obstructive pulmonary disease (COPD): Pronase is used to break down mucus in the lungs, which can help to improve breathing in people with COPD. 2. Chronic bronchitis: Pronase is used to break down mucus in the bronchial tubes, which can help to improve breathing in people with chronic bronchitis. 3. Emphysema: Pronase is used to break down mucus in the lungs, which can help to improve breathing in people with emphysema. 4. Sinusitis: Pronase is used to break down mucus in the sinuses, which can help to improve breathing and reduce inflammation. 5. Wound healing: Pronase is used to break down dead tissue and promote the healing of wounds. Pronase is available as a prescription medication and is typically administered by injection or inhalation. It is important to note that Pronase can cause side effects, including allergic reactions, bleeding, and infection, and should only be used under the supervision of a healthcare professional.
Animal embryonic development
Institute of Molecular Biotechnology
Timeline of human prenatal development
Embryonic stem cell
Preimplantation genetic diagnosis
Assisted reproductive technology
Clemens van Blitterswijk
S. Matthew Liao
Leukemia inhibitory factor
Blastocyst | Kinderwunschzentrum
Fetal development: MedlinePlus Medical Encyclopedia
Blastocyst Transfer- A Step Towards Single Healthy Baby, The Origin
Prenatal Diagnosis for Congenital Malformations and Genetic Disorders: Practice Essentials, Noninvasive Techniques, Invasive...
Generation of inner ear sensory neurons using blastocyst complementation in a Neurog1+/−−deficient mouse | Stem Cell Research &...
Effect of maternal obesity on estrous cyclicity, embryo development and blastocyst gene expression in a mouse model // Bond...
Better late than never: the clinical value of Day 7 blastocysts - Nuffield Department of Women's & Reproductive Health
Suboptimal culture conditions induce more deviations in gene expression in male than female bovine blastocysts | BMC Genomics |...
Selection of single blastocysts for fresh transfer via standard morphology assessment alone and with array CGH for good...
Application of Ovum Pick-Up, Intracytoplasmic Sperm Injection and Embryo Culture in Equine Practice | IVIS
Sea Lion Pup Born at Smithsonian's National Zoo | Smithsonian's National Zoo
IVF.net - Open Pulled Straw (OPS) Vitrification System • IVF Product Reviews at IVF.net
HS3ST6 heparan sulfate-glucosamine 3-sulfotransferase 6 [Homo sapiens (human)] - Gene - NCBI
Embryo Culture Media
WHO EMRO | Impact of the mandatory age-based single-embryo transfer legislation in Turkey on outcome of in vitro fertilization:...
Knockout mouse - Wikipedia
Stages of Development of the Fetus - Women's Health Issues - MSD Manual Consumer Version
A Review of Predictive and Contrastive Self-supervised Learning for Medical Images | Machine Intelligence Research
Search | The Embryo Project Encyclopedia
Volume 95 Issue 6 | Biology of Reproduction
Chimera research opens new doors to understanding and treating disease | The Scientist Magazine®
IJERPH | Free Full-Text | Hair Cortisol Concentrations as a Biomarker to Predict a Clinical Pregnancy Outcome after an IVF...
EvC Forum: A very brief history of Human Life
What is wrong with this flier?
- Blastocyst culture refers to growing the embryos in the laboratory for 4-6 days after fertilization. (theoriginfertility.com)
- Blastocyst embryos are considered to be robust. (theoriginfertility.com)
- BC is a technique in which deletion of a key gene for the development of a specific lineage creates a vacant niche (organogenesis-disabled phenotype) that can be complemented by the progeny of wild type pluripotent stem cells injected into embryos at the blastocyst stage of development. (biomedcentral.com)
- Although aCGH followed by frozen embryo transfer has been used to screen at risk embryos (e.g., known parental chromosomal translocation or history of recurrent pregnancy loss), this is the first description of aCGH fully integrated with a clinical IVF program to select single blastocysts for fresh SET in good prognosis patients. (biomedcentral.com)
- One widely used technique for creating transgenic mice involves the creation and injection of stably transfected mouse embryonic stem cells (ES) into mouse blastocyst embryos. (sigmaaldrich.com)
- In mouse embryos, cells seem prepatterned to become certain cell lineage because the first cleavage plane has been related with further embryonic-abembryonic axis at the blastocyst stage. (bioone.org)
- To achieve this, cells of in vitro produced bovine embryos were traced from the 2-cell stage to the blastocyst stage. (bioone.org)
Ball of cells called3
Days after fertilization4
- Since 1st September 2017, up to 12 fertilized eggs can be developed and observed for 5 days after fertilization (blastocyst stage) outside the body. (kinderwunschzentrum.ch)
- On average, 6 out of 10 fertilized eggs are healthy enough to develop into blastocysts 5 days after fertilization. (kinderwunschzentrum.ch)
- The blastocyst implants in the wall of the uterus about 6 days after fertilization. (msdmanuals.com)
- About 6 days after fertilization, the blastocyst attaches to the lining of the uterus, usually near the top. (msdmanuals.com)
- And as of the fifth day after fertilization, embryonic cells are no longer identical: one can recognize cells which form the embryo itself and cells that form the placenta (blastocyst stage). (kinderwunschzentrum.ch)
- Sea lions experience delayed implantation-also known as embryonic diapause-in which a fertilized egg, or blastocyst, may not implant in the uterine wall for several months following breeding. (si.edu)
- Embryonic stem cells are isolated from a mouse blastocyst (a very young embryo ) and grown in vitro . (wikipedia.org)
- Two cell lineages are observed on the embryonic-abembryonic axis of the blastocyst: the inner cell mass and the trophectoderm. (bioone.org)
- Blastocysts were then classified according to the allocation of the labeled cells in the embryonic and/or abembryonic part of the blastocyst. (bioone.org)
- Given their nonembryonic source, they could replace blastocyst-derived embryonic stem cells in research and medicine. (ca.gov)
- Preimplantation biopsy of blastocysts obtained by in vitro fertilization is an invasive technique. (medscape.com)
- This cost does not include the cryopreservation of additional blastocysts. (kinderwunschzentrum.ch)
- We used RNA sequencing to examine the effect of in vitro embryo production, in either serum-containing or serum-free media, on the global gene expression pattern of individual bovine blastocysts. (biomedcentral.com)
- RÉSUMÉ La présente étude menée en Turquie a évalué l'impact de la loi rendant obligatoire le transfert d'un embryon unique en fonction de l'âge et de l'augmentation consécutive des transferts d'embryons congelés-décongelés sur l'issue de la grossesse des patientes bénéficiant d'une fécondation in vitro. (who.int)
- Le transfert d'un embryon unique, le transfert d'embryons congelés-décongelés et le transfert de deux embryons ont été réalisés chez 5632 patientes après l'entrée en vigueur de la loi, tandis que l'approche traditionnelle par fécondation in vitro et par transferts d'embryons congelés-décongelés a été utilisée chez 6029 patientes avant le vote de cette loi. (who.int)
- RÉSUMÉ Pour étudier les effets de la silymarine sur le développement folliculaire, nous avons recruté 40 femmes en bonne santé subissant une fécondation in vitro (FIV) en raison d'une infertilité masculine. (who.int)
- Once the blastocyst reaches the uterus, it buries itself in the uterine wall. (medlineplus.gov)
- The blastocyst sticks tightly to the wall of the uterus and receives nourishment from the mother's blood. (medlineplus.gov)
- Inside the uterus, the blastocyst implants in the wall of the uterus, where it develops into an embryo attached to a placenta and surrounded by fluid-filled membranes. (msdmanuals.com)
- Blastocysts containing cells, that are both wildtype and knockout cells, are injected into the uterus of a foster mother. (wikipedia.org)
- KSOM allows for higher rates of cell division and produces higher yields of blastocyst development. (sigmaaldrich.com)
- A blastocyst is made up of an inner group of cells with an outer shell. (medlineplus.gov)
- We address these potential limitations by adopting the technique of blastocyst complementation (BC) to generate inner ear neurons from induced pluripotent stem cells (iPSCs). (biomedcentral.com)
- The wall of the blastocyst is one cell thick except in one area, where it is three to four cells thick. (msdmanuals.com)
- Some of the cells from the placenta develop into an outer layer of membranes (chorion) around the developing blastocyst. (msdmanuals.com)
- Since the 1950s, scientists have developed interspecies blastocysts in laboratory settings, but not until the 1990s did proposals emerge to engineer interspecies blastocysts that contained human genetic or cellular material. (asu.edu)
- This work validates the use of blastocyst complementation as a tool to create novel insight into the function of developmental genes and highlights blastocyst complementation as a potential platform for generating chimeric inner ear cell types that can be transplanted into damaged inner ears to improve hearing. (biomedcentral.com)
- Females are not pregnant until the blastocyst implants. (si.edu)
- This research is the first to produce induced pluripotent stem cell-derived inner ear sensory neurons in the Neurog1 +/− heterozygote mouse using blastocyst complementation. (biomedcentral.com)
- Mixtures simulating groundwater contaminants, insecticide formulation, and lawn-care herbicides reduced development to blastocyst and mean cell number per embryo (p or = 0.05). (cdc.gov)
- Hence the option of Blastocyst transfer should be based on the individual basis and circumstances. (theoriginfertility.com)
- The observed aneuploidy rate (44.9%) among biopsied blastocysts highlights the inherent imprecision of SET when conventional morphology is used alone. (biomedcentral.com)
- For patients in Group A ( n = 55), 425 blastocysts were biopsied and analyzed via aCGH (7.7 blastocysts/patient). (biomedcentral.com)
- Aneuploidy was detected in 191/425 (44.9%) of blastocysts in this group. (biomedcentral.com)
- For patients in Group B ( n = 48), 389 blastocysts were microscopically examined (8.1 blastocysts/patient). (biomedcentral.com)
- All patients had a single fresh blastocyst transferred on d6. (biomedcentral.com)
- [ 2 ] This technique has evolved throughout the years and is now largely performed by biopsy of the blastocyst trophectoderm cells with analysis using techniques such as next-generation sequencing (NGS) and comparative genomic hybridization (CGH) to test for aneuploidy. (medscape.com)
- In order to achieve this goal, it is expected that investigators will identify markers indicative of the state of the uterus receptive for blastocyst implantation, characterize how these markers are involved in the process of blastocyst implantation, and develop noninvasive methods for measurement of markers. (nih.gov)
- This Request for Applications (RFA), National Cooperative Program on Markers of Uterine Receptivity for Blastocyst Implantation, is related to the priority area of family planning. (nih.gov)
- 33. Molecules in blastocyst implantation. (nih.gov)
- The presence of fibroids can lead to decrease of fertility due to impairment of endometrial receptivity and abnormal implantation of the blastocyst. (hindawi.com)
- Combining in toto live imaging and various perturbation experiments, we demonstrate and measure fluid flow forces existing in the mouse blastocyst cavity and identify Klf2( Krüppel-like factor 2) as a fluid force reporter with force-responsive enhancers. (bvsalud.org)
- Blastocyst biopsy for PGD of mutation load and gender. (nih.gov)
- Advancements in embryo culture, blastocyst biopsy techniques, 24-chromosome aneuploidy screening platforms, and improved genomic coverage of new sequencing platforms, such as next-generation sequencing, have made PGT safe and accessible for all patients who undergo in vitro fertilization. (medscape.com)
- The modern approach to IVF involves blastocyst culture and biopsy followed by PGT and a single embryo transfer. (medscape.com)
- This is of utmost importance from the time of egg collection through to blastocyst stage and final embryo transfer. (planer.com)
- Genetic reporter for live tracing fluid flow forces during cell fate segregation in mouse blastocyst development. (bvsalud.org)
- Blastocyst Transfer prices from 350 kč - Enquire for a fast quote ★ Free consultation ★ Choose from 4 Blastocyst Transfer Clinics in Prague with 24 verified patient reviews. (whatclinic.com)
- A PGD case and analysis of blastocyst mosaicism. (nih.gov)
- High-throughput sexing of mouse blastocysts by real-time PCR using dissociation curves. (nih.gov)
- In a word, a blastocyst is an embryo that has developed for five to six days following fertilisation. (whatclinic.com)
- Pregnancy and live-birth rates (51% vs 38%) were higher in the two-blastocyst transfer group. (medscape.com)
- The authors concluded that the transfer of single, frozen-warmed blastocysts is associated with a lower risk for multiple gestation, preterm delivery, and low birth weight when compared with double blastocyst transfer. (medscape.com)
- Age at the time of vitrification and survival after thawing were similar in the one- or two-blastocyst transfer groups. (medscape.com)