Characterization and submitochondrial localization of the alpha subunit of the mitochondrial processing peptidase from the aquatic fungus Blastocladiella emersonii. (1/48)
In an effort to investigate the molecular mechanisms responsible for the drastic morphological changes the mitochondria go through during the life cycle of the aquatic fungus Blastocladiella emersonii, the gene encoding the alpha subunit of the mitochondrial processing peptidase (alpha-MPP) was isolated. Nucleotide sequence analysis revealed that the predicted alpha-MPP polypeptide comprises 474 amino acids with a calculated molecular mass of 51,900 Da, presenting a characteristic mitochondrial signal sequence. Northern blot analysis indicated a single 1.4-kb transcript encoding the B. emersonii alpha-MPP, whose levels decrease during sporulation, becoming very low in the zoospore, and increase again during germination. Despite these variations in mRNA concentration, B. emersonii alpha-MPP protein levels do not change significantly during the life cycle of the fungus, as observed in Western blots. Experiments to investigate the submitochondrial localization of B. emersonii alpha-MPP and beta-MPP were also carried out, and the results indicated that both subunits are associated with the mitochondrial inner membrane, possibly as part of the bc1 complex, as described for plants. (+info)Searching for the role of protein phosphatases in eukaryotic microorganisms. (2/48)
Preference for specific protein substrates together with differential sensitivity to activators and inhibitors has allowed classification of serine/threonine protein phosphatases (PPs) into four major types designated types 1, 2A, 2B and 2C (PP1, PP2A, PP2B and PP2C, respectively). Comparison of sequences within their catalytic domains has indicated that PP1, PP2A and PP2B are members of the same gene family named PPP. On the other hand, the type 2C enzyme does not share sequence homology with the PPP members and thus represents another gene family, known as PPM. In this report we briefly summarize some of our studies about the role of serine/threonine phosphatases in growth and differentiation of three different eukaryotic models: Blastocladiella emersonii, Neurospora crassa and Dictyostelium discoideum. Our observations suggest that PP2C is the major phosphatase responsible for dephosphorylation of amidotransferase, an enzyme that controls cell wall synthesis during Blastocladiella emersonii zoospore germination. We also report the existence of a novel acid- and thermo-stable protein purified from Neurospora crassa mycelia, which specifically inhibits the PP1 activity of this fungus and mammals. Finally, we comment on our recent results demonstrating that Dictyostelium discoideum expresses a gene that codes for PP1, although this activity has never been demonstrated biochemically in this organism. (+info)Chitin biosynthesis during Blastocladiella zoospore germination: evidence that the hexosamine biosynthetic pathway is post-translationally activated during cell differentiation. (3/48)
De novo construction of a chitinous cell wall accompanies Blastocladiella emersonii zoospore germination. At least an order of magnitude increase in total hexosamine occurs during germination. This increase is into polymer (chitin) and occurs on schedule in the presence of cycloheximide. Uridine-5'-diphospho-N-acetylglucosamine (UDPGlcNAc), both the end product of hexosamine biosynthesis and a substrate for chitin biosynthesis, is a potent inhibitor of the activity of the first pathway-specific enzyme of hexosamine biosynthesis in zoospore extracts. Certain uridine nucleotides, not perceptibly influencing the activity of the first enzyme per se, counteract the inhibitory effects of UDPGlcNAc. The concentration of UDPGlcNAc in the zoospore is sufficient to act as an inhibitor of the enzyme, but the amount of UDPGlcNAc is sufficient, by over an order of magnitude, to account for the chitin synthesized during germination. Both the production of UDPGlcNAc and its utilization for chitin synthesis appear to be post-translationally regulated in zoospores and during zoospore germination. (+info)Structure, expression, and functional analysis of the gene coding for calmodulin in the chytridiomycete Blastocladiella emersonii. (4/48)
The single calmodulin (CaM) gene and the corresponding cDNA of the chytridiomycete Blastocladiella emersonii were isolated and characterized. The CaM gene is interrupted by three introns and transcribed in a single 0.7-kb mRNA species encoding a predicted protein 91% identical to human CaM. B. emersonii CaM has been expressed in Escherichia coli as a fusion protein with gluthatione S-transferase (GST) and purified by affinity chromatography and cleavage from the GST portion using a site-specific protease. In the presence of Ca(2+), B. emersonii CaM exhibited a shift in apparent molecular mass similar to that observed with bovine CaM and was able to activate the autophosphorylation of CaM-dependent protein kinase II (CaMKII) from rat brain. CaM expression is developmentally regulated in B. emersonii, with CaM mRNA and protein concentrations increasing during sporulation to maximum levels observed just prior to the release of the zoospores into the medium. Both CaM protein and mRNA levels decrease drastically at the zoospore stage, increasing again during germination. The CaM antagonists compound 48/80, calmidazolium, and W7 were shown to completely inhibit B. emersonii sporulation when added to the cultures at least 120, 150, and 180 min after induction, respectively. All these drugs also inhibited growth and zoospore production in this fungus. The Ca(2+) channel blocker TMB-8 and the CaMKII inhibitor KN93 completely inhibited sporulation if added up to 60 min after induction of this stage, but only KN93 affected fungal growth. The data presented suggest that the Ca(2+)-CaM complex and CaMKII play an important role during growth and sporulation in B. emersonii. (+info)Esterase activity during the life cycle of Blastocladiella emersonii. (5/48)
Total esterase activity was measured in extracts on Blastocladiella throughout its life cycle by the degradation of alpha-naphthyl acetate. A fivefold incease in activity, apparently due to the synthesis of new enzymes, was found during sporulation. (+info)Characterization and expression of two genes encoding isoforms of a putative Na, K-ATPase in the chytridiomycete Blastocladiella emersonii. (6/48)
A P-type ATPase gene (BePAT1) from the aquatic fungus Blastocladiella emersonii, which surprisingly showed high similarity with the alpha-subunit of Na, K-ATPases from animal cells, has been reported recently [Biochim. Biophys. Acta 1383 (1998) 183]. In the present study, we describe the characterization of a second gene, denominated BePAT2, and show that these two genes have a different intron-exon structure but encode putative proteins with greater than 90% amino acid identity. Northern blot and multiplex reverse transcription and polymerase chain reaction (RT-PCR) assays have revealed that BePAT1 and BePAT2 genes have a non-coordinate, developmentally regulated expression during B. emersonii life cycle. Phosphoenzyme formation experiments using the immunopurified enzymes have indicated the presence of a Na, K-ATPase-like activity. Furthermore, immunofluorescence studies using B. emersonii zoospores localized the ATPases on the plasma membrane of these cells. (+info)Cloning and structural analysis of the gene for the regulatory subunit of cAMP-dependent protein kinase in Blastocladiella emersonii. (7/48)
We have isolated and characterized cDNA and genomic DNA clones encoding the regulatory subunit of cAMP-dependent protein kinase in the aquatic fungus Blastocladiella emersonii. Nucleotide sequence analysis has shown that the predicted protein comprises 403 amino acids with a calculated molecular mass of 44,263 Da and an overall 40% identity to mammalian RII subunits, including a serine in the phosphorylation site, which confirms the Blastocladiella protein as a type II regulatory subunit. The B. emersonii R gene presents two introns, one located in the 5'-noncoding region, whereas the other interrupts the coding region, just after the dimerization domain of the protein. The promoter region does not contain recognizable TATA or CCAAT sequences and is very GC rich, a characteristic shared by mammalian cAMP-dependent protein kinase subunit genes previously analyzed. S1 mapping and primer extension experiments revealed multiple transcription initiation sites. Several sequence motifs were identified in the 5'-flanking region which could be responsible for the regulation of this gene. (+info)Gene discovery and expression profile analysis through sequencing of expressed sequence tags from different developmental stages of the chytridiomycete Blastocladiella emersonii. (8/48)
Blastocladiella emersonii is an aquatic fungus of the chytridiomycete class which diverged early from the fungal lineage and is notable for the morphogenetic processes which occur during its life cycle. Its particular taxonomic position makes this fungus an interesting system to be considered when investigating phylogenetic relationships and studying the biology of lower fungi. To contribute to the understanding of the complexity of the B. emersonii genome, we present here a survey of expressed sequence tags (ESTs) from various stages of the fungal development. Nearly 20,000 cDNA clones from 10 different libraries were partially sequenced from their 5' end, yielding 16,984 high-quality ESTs. These ESTs were assembled into 4,873 putative transcripts, of which 48% presented no matches with existing sequences in public databases. As a result of Gene Ontology (GO) project annotation, 1,680 ESTs (35%) were classified into biological processes of the GO structure, with transcription and RNA processing, protein biosynthesis, and transport as prevalent processes. We also report full-length sequences, useful for construction of molecular phylogenies, and several ESTs that showed high similarity with known proteins, some of which were not previously described in fungi. Furthermore, we analyzed the expression profile (digital Northern analysis) of each transcript throughout the life cycle of the fungus using Bayesian statistics. The in silico approach was validated by Northern blot analysis with good agreement between the two methodologies. (+info)
Zoospore Germination in Blastocladiella Emersonii | Journal of Cell Science
I - Baier/Elstner/Hegemann/Schneider: Organization of SPP 1926
Chytridiomycetes: Features and Significance| Phycomycotina
Distinct sensitivity of fungal freshwater guilds to water quality
Joyce Hasegawa | Slippertalk Orchid Forum
Radioisotopes and Their Biomedical Applications | OMICS International
Biology-Online • View topic - Aquatic fungi on Spirogyra?
ALMA WHIFFEN BARKSDALE RECORDS
Aquatic Fungi from South Bass and Neighboring Islands in Western Lake Erie. I, Uniflagellate and Biflagellate Phycomycetes
Trouble in the Aquatic World | THE WILDLIFE SOCIETY
Fungicides as an underestimated hazard for freshwater organisms
Taste and Odor - North Texas Municipal Water District
Massapequa Mold Removal | Water Mold & Fire Restoration
Pink Pool Slime & White Water Mold: How to Get Rid Of Them - Pool Research
Alpha, Beta and Gamma Particles - Document in All Levels Physics
BrikWars Forums • View topic - Border Patrol (A Scythian Short)
BrikWars Forums • View topic - Border Patrol (A Scythian Short)
Biological Foundations 112, Lecture 24
Would using Softswim Oxysheen be the same as Baquacil CDX?
The anaerobic chytridiomycete fungus Piromyces sp. E2 produces ethanol via pyruvate:formate lyase and an alcohol dehydrogenase E
Presence of DNA sequences homologous to the ras oncogene in the aquatic fungus Achlya ambisexualis: possible role in...
Inhibition of a secreted glutamic peptidase prevents growth of the fungus Talaromyces emersonii
DNA Barcoding: Weekend reads
Listings in Party Suppliers & Equipment, Pet Stores, Fire, Water & Mold Restoration and Human Services in Supply and Shallotte ...
Aquatic Organisms: Microorganisms - Regional Aquatics Monitoring Program (RAMP)
PDF] An anaerobic mitochondrion that produces hydrogen | Semantic Scholar
The two Rasamsonia emersonii α-glucuronidases, ReGH67 and ReGH115, show a different mode-of-action towards glucuronoxylan and...
Listings in Party Suppliers & Equipment, Air Duct Cleaning, Fire, Water & Mold Restoration and Pet Boarding & Services in...
Global gene expression analysis reveals pathway differences between teratogenic and non-teratogenic exposure concentrations of...
Which of the following best compares rhizoids and... - OpenStudy
Ultrastructure of Thraustochytrium sp. zoospores | SpringerLink
Phytopathology 1987 | Superoxide Anion Generation: A Response of Potato Leaves to Infection with Phytophthora infestans
Water Mold Infection (Pythiosis) in Cats | petMD
Disaster Recovery with Water Mold Fire Restoration of Albuquerque Water Damage Restoration in Albuquerque, NM | Arkansas...
Ruokavirasto
Patent US5416010 - Olpidium zoospores as vectors of recombinant DNA to plants - Google Patents
Protist - Biology-Online Dictionary
Female - Encyclopædia Dæmonica
Molecular mechanisms underlying ochratoxin A-induced genotoxicity: global gene expression analysis suggests induction of DNA...
deppgaikwad1 - Page 2 - article directory
Mycologist Cardy Raper Talks Love, Fungi Sex and Cardi B | Culture | Seven Days | Vermonts Independent Voice
Unni Cecilie Nygaard - NIPH
Water Damage Restoration in Ormond-by-the-sea | Water Mold & Fire Restoration
What is phytophthora? | MNN - Mother Nature Network
Parasite of the Day: September 28 - Aphanomyces invadans
David R. Soll
Soll, D. R.; Sonneborn, D. R. (1971). "Zoospore germination in Blastocladiella emersonii: Cell differentiation without protein ...
D-glutaminase
Domnas A, Catimo EC (1965). "The behavior of amidohydrolases and L-glutamate in synchronized populations of Blastocladiella ...
Photoactivated adenylyl cyclase
Photoactivated guanylyl cyclases have been discovered in the aquatic fungi Blastocladiella emersonii and Catenaria anguillulae ... "The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling ...
Blastocladiomycota
As well, two (once) popular model organisms-Allomyces macrogynus and Blastocladiella emersonii-belong to this phylum. ... Genus Endoblastidium Codreanu 1931 Genus Polycaryum Stempell 1901 Genus Nematoceromyces Doweld 2013 Genus Blastocladiella ...
2016 in paleontology
... and Blastocladiella (Blastocladiomycota)". Mycologia. 108 (2): 303-312. doi:10.3852/15-224. PMID 26740543. S2CID 19514447. ...
Rhodopsin
"The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling ...
Blastocladiaceae
It contains the following genera: Allomyces Blastocladiella Blastocladia Blastocladiopsis Microallomyces The family was ...
List of MeSH codes (B05)
... blastocladiella MeSH B05.283.600 - neocallimasticales MeSH B05.283.600.600 - neocallimastix MeSH B05.283.600.675 - piromyces ...
Metabolism and morphogenesis in a new Blastocladiella | SpringerLink
Análise da expressão gênica global durante a germinação do fungo aquático Blastocladiella...
... realizado um programa de seqüenciamento de cDNAs obtidos de bibliotecas da fase de germinação do fungo aquático Blastocladiella ... Análise da expressão gênica global durante a germinação do fungo aquático Blastocladiella emersonii ... Globlal gene expression analysis during germination of the aquatic fungus Blastocladiella emersonii ... realizado um programa de seqüenciamento de cDNAs obtidos de bibliotecas da fase de germinação do fungo aquático Blastocladiella ...
Zoospore Germination in Blastocladiella Emersonii | Journal of Cell Science
PDF] The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP...
We showed that the microbial rhodopsin of Blastocladiella emersonii is a rhodopsin-guanylyl cyclase (RhGC), a member of a ... The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast optical control of cGMP signaling. ... article{Scheib2015TheRC, title={The rhodopsin-guanylyl cyclase of the aquatic fungus Blastocladiella emersonii enables fast ... a retinylidene/guanylyl cyclase fusion protein and optogenetics tool from the aquatic fungus Blastocladiella emersonii ...
Comparative Genome Sequencing Page 1
Category:Translingual proper nouns - Wiktionary
Estudo da expressão dos genes de choque térmico hsp90, hsp60 e hsp10 do fungo aquático...
Study of the expression of heat shock genes hsp90, hsp60 and hsp10 of the aquatic fungus Blastocladiella emersonii ... Estudo da expressão dos genes de choque térmico hsp90, hsp60 e hsp10 do fungo aquático Blastocladiella emersonii ... Blastocladiella emersonii. Blastomyces (Estudo). Choque térmico. Expressão gênica. Fungo zoospórico. Fungos (Estudo). Hsp. ... Blastocladiella emersonii. Blastomyces (Study). Fungi (Study). Gene expression. Hsp. Thermal shock. Zoosporic fungus. ...
OBTAINIUM-Digitizing the Reel Life films of Lynn Margulis by James D. MacAllister -
Kickstarter
Table of Contents - August 11, 2015, 8 (389) | Science Signaling
Guanylate cyclase - Wikipedia
David R. Soll - Wikipedia
Candida albicans Lacking the Gene Encoding the Regulatory Subunit of Protein Kinase A Displays a Defect in Hyphal Formation and...
Cloning and structural analysis of the gene for the regulatory subunit of cAMP-dependent protein kinase in Blastocladiella ... Blastocladiella emersonii (22), and mouse (33), we designed a set of oligonucleotides for PCR amplification of a region of the ... Blastocladiella emersonii (accession number M81713); A.n., Aspergillus nidulans (accession number AF043231); M.g., Magnaporthe ...
Candida albicans Lacking the Gene Encoding the Regulatory Subunit of Protein Kinase A Displays a Defect in Hyphal Formation and...
Types of Fungi (Classification System)
TCDB » SEARCH
Unifunga/Deutschland - Wikiversity
D-glutaminase - Wikipedia
You searched for Australia Online Pharmacy ⭐ www.HealthMeds.online ⭐ Mg Levitra Australia - Online Purchase Levitra Australia -...
Using a Robust and Sensitive GFP-Based cGMP Sensor for Real-Time Imaging in Intact Caenorhabditis elegans | Genetics
Home - Maine Chytrid Laboratory - University of Maine
Diversity of Basidiomycetes in Michigan Agricultural Soils | Applied and Environmental Microbiology
Oprian DD[au] - PubMed - NCBI
Ultrastructure of Thraustochytrium sp. zoospores | SpringerLink
Currículo do Sistema de Currículos Lattes (Marcio de Castro Silva Filho)
Labpib:Ricardo Z.N. Vencio - OpenWetWare
Schizorhodopsins: A family of rhodopsins from Asgard archaea that function as light-driven inward H+ pumps | Science Advances
Abbreviations that start with X, Y, and Z - AskDocWeb.com
Crystallization - Experimentelle Biophysik
Synthesis of ribosomal proteins during sea urchin early embryonic development | Springer for Research & Development
OPUS Würzburg | Characterization of novel rhodopsins with light-regulated cGMP production or cGMP degradation
A light-activated guanylyl cyclase opsin was discovered in the fungus Blastocladiella emersonii which was named Cyclop (Cyclase ... Ein lichtaktiviertes Guanylyl-Cyclase- Opsin wurde im Pilz Blastocladiella emersonii (Be) entdeckt, das von Gao et al. (2015) ... A light-activated guanylyl cyclase opsin was discovered in the fungus Blastocladiella emersonii which was named Cyclop (Cyclase ... Ein lichtaktiviertes Guanylyl-Cyclase- Opsin wurde im Pilz Blastocladiella emersonii (Be) entdeckt, das von Gao et al. (2015) ...
Emersonii12
- Foi realizado um programa de seqüenciamento de cDNAs obtidos de bibliotecas da fase de germinação do fungo aquático Blastocladiella emersonii. (usp.br)
- We conducted large scale cDNA sequencing from libraries obtained using RNA from germinating cells of the aquatic fungus Blastocladiella emersonii. (usp.br)
- Cantino, E. C., Truesdell, L. C.: Organization and fine structure of the side body and its lipid sac in the zoospore of Blastocladiella emersonii . (springer.com)
- Adelman, T. C., Lovett, J. J.: Synthesis of ribosomal protein without de novo ribosome production during differentiation in Blastocladiella emersonii . (springer.com)
- A light-activated guanylyl cyclase opsin was discovered in the fungus Blastocladiella emersonii which was named Cyclop (Cyclase opsin) by Gao et al. (uni-wuerzburg.de)
- Here we implement a guanylyl cyclase rhodopsin from Blastocladiella emersonii as a new optogenetic tool (BeCyclOp), enabling rapid light-triggered cGMP increase in heterologous cells (Xenopus oocytes, HEK293T cells) and in Caenorhabditis elegans. (uni-wuerzburg.de)
- In 2014, a Brazilian group showed that a rhodopsin fusion with a guanylyl cyclase is expressed in the aquatic fungus Blastocladiella emersonii and is activated by green light (Avelar et al. (uni-wuerzburg.de)
- Large-subunit rRNA sequence of the chytridiomycete Blastocladiella emersonii and implications for the evolution of zoosporic fungi. (microbiologyresearch.org)
- Decay of gamma particles in germinating zoospores of Blastocladiella emersonii. (termsreign.gq)
- Growth and differentiation of the water mold Blastocladiella emersonii: cytodifferentiation and the role of ribonucleic acid and protein synthesis. (courtfield.ml)
- The recently discovered rhodopsin-guanylyl-cyclase (RhGC) of the fungus Blastocladiella emersonii offers an elegant solution to this problem. (spp1926.org)
- Luévano-Martínez LA, Caldeira da Silva CC, Nicastro GG, Schumacher RI, Kowaltowski AJ, Gomes SL (2019) Mitochondrial alternative oxidase is determinant for growth and sporulation in the early diverging fungus Blastocladiella emersonii . (mitofit.org)
Fungus1
- The proteins in this group allow to feel the direction of the light and other microorganisms that have simple eyes - such as Chlamydomonas and fungus Blastocladiella, floating spores which also provided with photosensitive sensors. (ublogz.com)
Allomyces2
- Allomyces, Catenaria, Blastocladiella) to the Blastocladiomycota. (umaine.edu)
- iii) Various species of Allomyces and Blastocladiella have been found to be valuable research tools in studying morphogenesis. (biologydiscussion.com)
Fungi1
- In other cases of the saprophytic fungi such as Rhizopus and Blastocladiella the rhizoids develop which penetrate the substratum and absorb the food material. (yourarticlelibrary.com)
Fungal1
- a - dinoflagellates family Warnowiidae, b - chlamydomonas, c - fungal spores Blastocladiella. (ublogz.com)
Production1
- One phase of morphogenesis (production of resistant sporangia) in the life cycle of Blastocladiella has been investigated experimentally in some detail, and the underlying biochemical transformations have, in part, been elucidated. (springer.com)